LOCUS       DAOSOX010000001        40374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1032_length_40374_cov_8.758972, whole genome shotgun sequence.
ACCESSION   DAOSOX010000001 DAOSOX010000000
VERSION     DAOSOX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 40374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40374
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1032_length_40374_cov_8.758972"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="PLX22_00005"
     CDS             <1..128
                     /locus_tag="PLX22_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT18318.1"
                     /translation="CPTWMRAEKLISIAHPQFQDDLIKQAEKMKIWTRTNKQIPV"
     gene            complement(274..771)
                     /locus_tag="PLX22_00010"
     CDS             complement(274..771)
                     /locus_tag="PLX22_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18319.1"
                     /translation="MALHDRLYNYIITLLSLKTIDVKKDWDMTKRMNDYVNAMIEEWG
                     SDNTFYGLSHEGQLIEQWTTDLDGLEIYYSYVRNSKWVKVIVLPTETGICPERDIYKW
                     KGYINEWIAETAVWWAFELLTENEAKAFMIKNKPVVKFQFIRLGHPHELTAKFDGNYW
                     VVIDD"
     gene            complement(813..>1486)
                     /locus_tag="PLX22_00015"
     CDS             complement(813..>1486)
                     /locus_tag="PLX22_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18320.1"
                     /translation="ELAEAQKRTEQRVDALTVKMEELVEAQKRTEQRVDALTVKMEEL
                     AEAQKRTEQRVDALTVKMEELAEAQKKTEVVVQQLLYDMNMVKKQLGGLSHAVGYGIE
                     DKVIPFMESFIEKEYGFVPQEVERKFIKYDQKNKDEINIFVKGLKNDVTMIVVGECKS
                     QPGKSDIDDFNKMLHRLQSHFKQDISGFIVGYSFDPEVEDYLITHYPHIRYYKTYQIE
                     RLARK"
     assembly_gap    1487..1586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1587..1776)
                     /locus_tag="PLX22_00020"
     CDS             complement(<1587..1776)
                     /locus_tag="PLX22_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18321.1"
                     /translation="MPYSVKIYKHLDELEPKVKRVIMELLEEIDKNVKESVKKEDFND
                     LKNIVARLADAQEKTEQKI"
     gene            complement(1976..3181)
                     /locus_tag="PLX22_00025"
     CDS             complement(1976..3181)
                     /locus_tag="PLX22_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOT18322.1"
                     /translation="MATGIKDKVAIIGMGCTKFGERWDADANALMVEAYNEAIADAGI
                     EPGEIQAAWVATCFDEINVGKSALAASIALRLPNIAVTRVENFCASGTEAFRGAVYAV
                     ASGAYDIVLAIGCEKLKDIGYGGLPEFSQALGILNGLWFPNVTAPGCFAMLAGGYAAK
                     YGLKMEDVKRAMAHISVKSHANGALNPKAHLQRPVTEEQVLQSPMIAYPLGLFDCCGV
                     SDGAACAIVCSVDVAKAMKKKDFVTVKALQISVSNGEEMAFSNWDGAHVVNTEKAAIR
                     AYAEAGIKNPREELSLLEVHDCFSITELVTMEDLHISERGKAIDDVMDGFFDSTGKIP
                     CQIDGGLKCFGHPIGASGLRMVYEIYLQLHGRADKRQLKNPDIGLTQNLGGFPMMNVI
                     SVAIVGRYT"
     gene            complement(3192..4634)
                     /locus_tag="PLX22_00030"
     CDS             complement(3192..4634)
                     /locus_tag="PLX22_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT18323.1"
                     /translation="MIGIVSYGGYIPLRRLSRMQVVQQMAWYNSLLYSLMTGERSVAN
                     WDEDAITMAVAAAVDALQNVEQKSIDAVYAASTSFPFDDRQNAAVIAEALNCRNNVRT
                     VDMAGSLKAGVSAVVAALDAVESDSANKVMVTAGERRFTRAASTLELMYGDGAAALVV
                     GRENVIAECIGSYSYHEDFVDHYRGADKRFDYQWEERFIRDEGFGRIIPTAIKLFCDN
                     YHTDINEFACVIYPCPFAREHAGIAKKIKSAHVYTNLHDGVGDTGVAHPLVMLCHALD
                     SAKPGDKILVVGFGQGCEIVAFKVTDAIESFKRPRGITRSIANKKDIPYPKYLKFREL
                     INADTSIRAEAVPNTSLTALWRHRKMLNGFVGYKCKACGTPQFPPYPICVNPACNKSG
                     AMDDYVFSDKMGTVLMYTGDMLAASVEPPALYGLVEFDGGGRTLLDFTDCELEKLNVG
                     TKVAMSFRIHRQDSMRGFTGYFWKAVPIME"
     gene            complement(4751..5653)
                     /locus_tag="PLX22_00035"
     CDS             complement(4751..5653)
                     /locus_tag="PLX22_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT18324.1"
                     /translation="MSIKLDGKVAVVTGAGSGIGRTYALEFARRGAMVVVNDLGSLRD
                     GSGANHSAADAVVEEIKKAGGKAVANYDSVATVQGGENIIKTAMDAFGRVDIVVNNAG
                     ILRDKTFVKMTEDEWDIVVSVHLRGAFCVTAPAFKIMREQGWGRIIFTASGAGLYGNF
                     GQTNYASAKMGLVGLMNALKLEGAKYNILVNTIVPVAASRMTEDVLPKEIFEKLKPEF
                     VTPLVMYLCSDECKDTGMLINCGGGWYSRAAIVCASGTIIQGKDEITAEDIMAQWSKI
                     TDLSDAKPLNNIGETFAYMAPLLK"
     gene            complement(5681..6529)
                     /locus_tag="PLX22_00040"
     CDS             complement(5681..6529)
                     /locus_tag="PLX22_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT18325.1"
                     /translation="MPLNETCIGKKYKSDPIKISQHESIYYALAYNEDNDAYFDNRRQ
                     GGIIAPPMYAVFYAAGPTAQLMFDAEIGINMMFVVHYSQYFEWIKAVKPGDEVTSEGT
                     ITHITVKEKGSILGWETVTKNQHDDIVVRAQWEFFDRSAGSGKPDGKAEEGKIPSQFI
                     FDDAMRVRNGQTYIYAEPSGDHNPIHVDDDFAKKVGLPGIILQGLCTMAFAHKMAVDH
                     LTPDRDPLKVKELYVRFARPVLPGQVLTFKGYTMEKLAEKTKYGIVALNDSGKDVLRN
                     GWCTVA"
     gene            6852..8222
                     /locus_tag="PLX22_00045"
     CDS             6852..8222
                     /locus_tag="PLX22_00045"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT18326.1"
                     /translation="MPHNDVKRYYNHDQGIQTLKVSKIITPLTAVVIALFLYQDIYIL
                     HFYFTFWYRFIPIMLCIVYFLLTLSLLKRHVHYVIPLYTLLLLSGIIMILGIAYRIFT
                     NPLYPETFLSNVSTGIQTVFIVLFLIASGARRFLPIVYTVSLVLFLISLSFNKQLTSL
                     ELSALSNPIATTLFLSLLAYVFESMNSREFALRFTVEQNEDELNQYKDTMEKELAFAS
                     YLHRLIMPLKPPVPSISVYYHPYHNLGGDFYDFIPLDKKKDRIGIFISDVTGHGVSSA
                     FITATLKHYLVVGKKYHENPQKLMYYLNEYLTGNIGQNFVTALYCIIDFNKKELIYSN
                     AAHYAPIIVGKDGNIHELEKTGKPFPLGIIKKRKLIKYSKNFSYSTIQLQQGSKLVLY
                     TDGIIELYNAGNPSNFDTGVFFQLLQQYHLLSSADMLNEIDRHLTSQHGNNYINDDIC
                     VIVVDI"
     gene            complement(8225..8683)
                     /locus_tag="PLX22_00050"
     CDS             complement(8225..8683)
                     /locus_tag="PLX22_00050"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOT18327.1"
                     /translation="MNKKISILFLCTGNSCRSQMAEGFANALRGDIIEAYSAGIEPKT
                     VDPLAIKVMKEAGVDISHHVSKHVNELINNQFDFVITVCGHAYENCPVFPGKTKVMHR
                     GFEDPPQLAGEAKTQEEALYHYRKVRDQIKAFILELPEWLDKEKTEFIMS"
     gene            complement(8858..9901)
                     /gene="arsB"
                     /locus_tag="PLX22_00055"
     CDS             complement(8858..9901)
                     /gene="arsB"
                     /locus_tag="PLX22_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOT18328.1"
                     /translation="MADGVAKKLSFVDRFLTLWIFFAIGAGVAIGYFLPEIKTFIYSL
                     QSGTTNIPIAIGLILMMYPPLAKVKYEEMGKIFQNKRLLGISLLLNWVVGPIVMFGLA
                     VILLRGYPEYMVGVILVGLARCIAMVIVWNELAGGDRELAVGLVAFNAVFQVLFYAVY
                     IYLFITLALNWFGLAEGLDISISISESAKTVFIYLGIPFLAGVATRFGLISYMGKQWY
                     EAKFIPRISPLTLIALLFTIIVMFSMQGNKIIERPQDVVIAALPMLLYFVLMWFVTFY
                     IIKKITGDYSKTAAVAFTAGSNDFELAIAVAIAIFGINSGQAFATVIGPLVEVPVLIG
                     LVDVALVFKKRYF"
     gene            complement(9970..10203)
                     /locus_tag="PLX22_00060"
     CDS             complement(9970..10203)
                     /locus_tag="PLX22_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT18329.1"
                     /translation="MKKFEILGTGCSKCTTLQHLTEQAAKELGIPYQLNKVTDIKQIM
                     SYGVMITPALVVDGQVKIAGKVPSIEEIKKLIQ"
     gene            complement(10249..11547)
                     /locus_tag="PLX22_00065"
     CDS             complement(10249..11547)
                     /locus_tag="PLX22_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT18330.1"
                     /translation="MDWKKELKICMYMVIAFVIFFHLPAGNPRFDNAVSEALKLTRWY
                     AREHVLLCLVPAFFIAGAIATFVSQNSVMKYLGPAAPKLMAYGVASVSGTILAVCSCT
                     VLPLFAGIYTMGAGLGPATAFLYSGPAINVLAIILTARVLGLEIGIARAVGAIVFSIF
                     IGGLMYLIFRKEEIQKANAMMNMPQPETSRPLWQTVIYFLLMVAILVFVNWGKPDAPE
                     GIWYYVYSYKWEITGVVSLLFGLVLWAWFTVTWWKIVIAIIVTVMSGFLFPHRPLIPF
                     VAGIIALIAIGITEKGELLTWLEQTWSFAKQILPLLLGGVLAAGFLLGSPGHEGIIPS
                     QWIAMAVGGNSFFANFFSSVVGAFMYFATLTEVPILQGLLGNGMGHGPALALLLAGPT
                     LSLPSMLVIRSVIGTQKTAVYVSLVIVMATITGILFGLLW"
     gene            complement(11570..11917)
                     /locus_tag="PLX22_00070"
     CDS             complement(11570..11917)
                     /locus_tag="PLX22_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT18331.1"
                     /translation="MDTMTQKKYEARAAVMKALAHPTRLYMVEELQKGAQCVCKLQEL
                     IGADMSTVSKHLSVLKSAGLVYDKKEGTTVYYFLKMPCVSEFISCIDTVIQRNATEQL
                     EVARCCINSTQQL"
     gene            complement(12780..12956)
                     /locus_tag="PLX22_00075"
     CDS             complement(12780..12956)
                     /locus_tag="PLX22_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18332.1"
                     /translation="MPRIIRKVRRTVCAVAESKVYALTTMSQPSKKDSSIESIMEATR
                     MRYRRGVVRMYLNS"
     gene            complement(13564..13637)
                     /locus_tag="PLX22_00080"
     tRNA            complement(13564..13637)
                     /locus_tag="PLX22_00080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13601..13603),aa:Arg,seq:tct)
     gene            complement(13681..13755)
                     /locus_tag="PLX22_00085"
     tRNA            complement(13681..13755)
                     /locus_tag="PLX22_00085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13718..13720),aa:Pro,seq:tgg)
     gene            complement(13834..14640)
                     /locus_tag="PLX22_00090"
     CDS             complement(13834..14640)
                     /locus_tag="PLX22_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOT18333.1"
                     /translation="MGKPFFDKPARIFAHRGNSREYPENTALAFEKAIELGADVIETD
                     AHITKDGYVVIAHDEELSNITDGNGKIGELPLLQIKQLDAGYNFTQDGETYPFRGKGI
                     TIMTLEEMLETFPHQRFNVDLKDNNILLAEKFCDVIEKHNAQHRVVGASQHGKNLAYI
                     RKRLPGMATSFGLWGVLGFYFLFRSGLLGLFKQFSGDALQIPEFIGPSHLANGTLVRM
                     AHERGIQVDVWTVNSERDMRRLLDIEVDGIITDDIELLKKVLQDYPVEGE"
     gene            14858..15010
                     /locus_tag="PLX22_00095"
     CDS             14858..15010
                     /locus_tag="PLX22_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18334.1"
                     /translation="MIQNDMPIPRRYPLIQLRTAQAASPQNDDNNPFSSLTGGNEPAV
                     GNASIK"
     gene            15237..15566
                     /locus_tag="PLX22_00100"
     CDS             15237..15566
                     /locus_tag="PLX22_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18335.1"
                     /translation="MKYGKTIFIIIAILSTIFIIGEIGTYIYVEAPLSTDFYSSIPKK
                     DVYKLQNHYGIKVVTGNNWIHLGWIANPDEEYYTIELKNVHGTVKKVGTTQFGSFLFH
                     GKGATSG"
     gene            15644..16354
                     /locus_tag="PLX22_00105"
     CDS             15644..16354
                     /locus_tag="PLX22_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18336.1"
                     /translation="MCVPFITDNYRTLFIPEHSGYYINDHTIFQDAYGNWQLIGITSK
                     TDGNPDAEKYFAVASSKTFPPNDIMKEESPVADVGELAWAPHVIKHKNIYHMFWSPHK
                     LHHMTSTNGIRWGNHDVIMEKPYHKFFRDCMILQVAKDQWLLYTTARGTFFSQIDVYQ
                     SFNLTQWQYIRTALQSSWGSERNSPALITTLKAHAPEWIYVPQTKQWYITTCGWKWVA
                     TLTNGEAAIAPVSWKCKK"
     gene            complement(16363..19287)
                     /locus_tag="PLX22_00110"
     CDS             complement(16363..19287)
                     /locus_tag="PLX22_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18337.1"
                     /translation="MKKIIRYVCYGILLVVFVYACGYMVIPKLLTIGKHSNHNVYVSL
                     GFHVNMYHSYRIDTNDEAGFGKDIRVIRKIIEVLDEKNKKGIPVKGVWDFENLFTLEE
                     ILPQYAPDIIANIKRRVKEHGDEIILMSYNNGLVSAMTQDECMYSVKYAISNSRRSGV
                     KDIFKDYSPFVRPQEMMATSGNYELYKQLGLQGIVLYYSGIPFDAFRVFVDELSLEEA
                     HNPLMYHNKNTHEKMMVIPAYNQADLIENVSIYNWVHMLRYQQLRGTINNDVMICINA
                     DADDSYWYGYDLPSYLKWLPNTGGLSQLIDDIASLDFVTFTTIGDYVKNHKPVKEISF
                     GQDTADGSFNGYVSWAEKKYCSDYWTAVEKDRNIHKLAEAYYSFIGQSAPGDITTALK
                     RSFEKRMRLLSTTNFGMATPFLAKNREHVVETITHSMESLTKPVVADIKKGAKGIIAK
                     QAINTNDGYEYVTSLLLLQQDTAPFVFKLPMELPQGYTYYMQSKDMLTPVNMFSSNGV
                     STIFIGRQPLHSGVYHIVRTQPGTKHYAAEATDTMIKNNTITVTVDNGFIQGVEAHGS
                     TILNKMSLIPWIAYNGQDYRPEKITASVLSDGKNGVAAVALRGEIALPENVTRGYFDY
                     TIYLVDDIDMLFVEGTITYPDTPRTTIFKPGMPALARIYDERWQQVIPCPLFAAMTAT
                     KDIPFTVIKKNYFGVEDTYKIDYFKHSKENLRLANVNNHITAGYVAVTNSKEFIAVAK
                     DNEVLSNFAFCPLKIDYGMVKGFTVSLNPFGTFFGRQYFQPTWGNGQGFKSAILNGDQ
                     YHSGACTYSGTTQRFSLAIAHGRGALQQSVKAQLQSFAHQPFIVAMNEVTPVQQKQKL
                     QSPKGVVALYNNGGVYINFEKVDGAAGYNIYCGNKDALKKIGVTSNTSFFAKNNWDGK
                     PFVEGNTYVAAVSAYDDNGNVNKVPVTYTFVAKPVKESLDLPLTLQLKILYHCLVAQL
                     RW"
     gene            19739..20665
                     /locus_tag="PLX22_00115"
     CDS             19739..20665
                     /locus_tag="PLX22_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOT18338.1"
                     /translation="MKKQLTVMTLFHVLIFFFSISCSSKTENHVTPIVQNILYTKTAI
                     EDYLKNRINQQSSPILFASFWDFDGTIIKGDCSEGLQEENKQVYKGLVQVAIENNFSK
                     KYKNNEYNKFLQHYAYLETHKGHSDAYIYLATLFTGRDIRDLQQLSQHYFSHTLRYYF
                     FASSIAIMNQLKAHGIKIYIISASPDYFVKGAAYSLTVSADHIYGIKIKTNNGIATEQ
                     VIPPLTYAEGKTKLLKSIIDRLKKENKTNHVYAIAAFGNSYHTDGNFLNYVATQTLPA
                     GKPVSVMINGGSPPKKYHALFECVIQNDVIAK"
     gene            20670..21608
                     /locus_tag="PLX22_00120"
     CDS             20670..21608
                     /locus_tag="PLX22_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT18339.1"
                     /translation="MKRYAIFFFIILFGLLPLCTPAQNQWGYYCKDTYSNNSKHYWDN
                     TLKSLSVLCFTGITISKNACNIPSFSNEHYLFTKAQQYNIAIIPHVTFTSVTEGMSFL
                     KAREWDNVITTLANALHTNAWAGIHFDFEYLPAAYSNAYAQFLKKFRSIAPDIKLSAA
                     LFPQIEFHKEHAAFHNYEKLSPFLNEVVIMCYDYHNPKTKPGPVTDMQWTMKNIEYAL
                     RFFKAHQIWLGMPAYGYMWKNGKYHSVITTRAIPKYTSIYSNYRHSSGTLCIEYIKND
                     DMFIALVSDTVTIKKLQHLATVYSLKGTALWRLGFE"
     gene            complement(21659..22768)
                     /locus_tag="PLX22_00125"
     CDS             complement(21659..22768)
                     /locus_tag="PLX22_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT18340.1"
                     /translation="MKFLKGFVYMAMMTFATAAFAQEAVKEEIKDIKISGKAYLEWNK
                     ELSNYDDTTEHQNTFEIKRVYLDFKKEFDSVWSARVTTDVGQVESEDEIEVTDTQSGN
                     PETVDVKAKSDPYTVYVKYAYIQAKQKMDFVDANFKFGMIGTPLIGLIDDKSDYRWVE
                     QNYVDQAKNIIGTSIDSSADMGVSVDLSFFKMVKLSGSLVNGEGYKKVNEDELGDDGK
                     AYYGMLTITPVKGLDIFGTYRNQITDDGHGSDNYSRYMAFGASYSTDLVKVGAMYVMP
                     EVKKGGVENEYTLVDSWLLANLESVINIPILITGRYSYGENEDVDDSEVTIWAAGLGY
                     AINKNIRVMAYYQDADNDAKEDPETTFYVKTEVVF"
     gene            23188..24099
                     /locus_tag="PLX22_00130"
     CDS             23188..24099
                     /locus_tag="PLX22_00130"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOT18341.1"
                     /translation="MKPNNLTAEQYSIFKENGTEPPFNNAYWNKKEHGIYVDIVSGEV
                     LFSSLDKFDSGTGWPSFTKPVNNNAVITCSDPSHGMIRTEVRSKSSDGHLGHVFADGP
                     APAGLRYCINSASLRFIPVKKIIEEGYNDYIYLFENTPIGFEQIIIGGGCFWGVEAYF
                     KKVKGILDTTVGYSGGTIKNPDYTMVCTGTTGHAETVLITFNPDIVNLERILKHFFSI
                     HNPSTLNRQGPDIGTQYRSALYYINDNQKITIDTIIQQITSRGAIVTTEVAKAINFFK
                     AEEYHQDYLDKNPGGYCHINLNKIYQV"
     gene            complement(24102..24764)
                     /locus_tag="PLX22_00135"
     CDS             complement(24102..24764)
                     /locus_tag="PLX22_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT18342.1"
                     /translation="MSSFSSYDIPKTDNNDVFQRFTEYFHKPSASHVDSLSDKLRFFR
                     QHNNLSYEQLAAITGICVELLQKIEERRAYPSLRVIHQLTRSLQQAKVIAPGTPVEWG
                     YTVIKKDDVIQLKNERPKNIFLNNVEPSHTADEADVNEVVAVKAYIITLEGNYTKKDF
                     SIHNGEEFIHVKKGSIKITLGSNEVILNHGDSIYFLSLIPHKIENVASSESVIHVIVY
                     QE"
     gene            24987..25910
                     /locus_tag="PLX22_00140"
     CDS             24987..25910
                     /locus_tag="PLX22_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18343.1"
                     /translation="MKIPFSILSITEKALTKSMDVHTMERIAKSIIPHYDLHKIMGFS
                     ESMPIPNKDAASQIVHDFSESKYFFKLVCALMEAQEYGIMGRRYPIAHLHEIVTEIIN
                     LGFIFDKNNHIFIENANTRKTQHWGVLEENEEYIITLLRMDIAANTQLVRKYTPPLIA
                     HTYQNIQTIVTSAVEQRNGRIWNWEGDGCIAAFILGNKNMTAVFAAIEILHELFLFNE
                     LVSKLPEAVNARIAIDTGYCAYKHSSEELKKTELVKKIMEIESHYTPINAITISNTIY
                     ASLHPDIAKLFILTRKSATPYYTHTVKWEDI"
     gene            25907..26869
                     /locus_tag="PLX22_00145"
     CDS             25907..26869
                     /locus_tag="PLX22_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18344.1"
                     /translation="MNITVLYHKKNASSLQKTFGTLMKSKKYSIEFIDRDEYNKPKKT
                     TANSLYYLDITGLQQADIQKYIKYFNKESMFYGIIDYKNTFSDIAWFFHNNACDYITK
                     ETCNNPISESRIRAIVTFKSITLSETPQKKYIIARDSWKSIKPEQEYTFCFLLIEILD
                     VHELKKHIASEHLDSKIFSFRHFVENTLNQLDGKLWMWMDATGLFIFPFDGKSLPAVD
                     SAIRLTVNRSIISLEHTLFNQIIHYRIAMHIGNTVYQPHGKTGTIISDCINSLFHLGQ
                     KYTADEGLFITEDVYNVLNSRTKGMFIQTHLFEGRQIYRCKNII"
     gene            complement(26875..27180)
                     /locus_tag="PLX22_00150"
     CDS             complement(26875..27180)
                     /locus_tag="PLX22_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOT18345.1"
                     /translation="MNNIHRRKVKYLKACAHGLDPVVLIGKNGLSKAVLNAIDEVLLC
                     HELIKIKFIDCKEERKELARQIVEKTGSNYITMIGNVLIIYRQHPEADKRQYELKAI"
     gene            27651..28421
                     /locus_tag="PLX22_00155"
     CDS             27651..28421
                     /locus_tag="PLX22_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT18346.1"
                     /translation="MSNDTILTKDPGFRYHEKAPKIDAKEFQKVIDSRRSVRIYEKEP
                     IPDKIIQQCLRNALLAPNSSNLQPWEIYHIATPEVRQQMNKACLEQIAAKSAAALFVA
                     VARTNTWKEHAKEMVQLFEAAGNVPKSAIAYYKKLVPFVYNQGLFGIWGLIKRILFFI
                     RGLKTPVIHEPVSHCHMKIWAVKSTALACQNLMLSLRAYGYDSCPMEGYDSKLIKKIL
                     QLPEDAVVVMVISAGKRAIGGIYGPRIRYDENRFLKRV"
     gene            28428..29237
                     /gene="zupT"
                     /locus_tag="PLX22_00160"
     CDS             28428..29237
                     /gene="zupT"
                     /locus_tag="PLX22_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOT18347.1"
                     /translation="MLHNPNVVFAFILTLLAGLATGIGSAISIISKKTNTKVLSLALG
                     FSAGVMIYVSMMEILSKSFDVLYHEYGKSGGWFALISFFGGIALTGIIDIIVPSAENP
                     HEIKRVETMPLPATNREQQLMRLGLLSALAIGIHNFPEGLATFVAALHDTKVGIPIAI
                     AIALHNIPEGIAVAVPIFHATKSRRKAFVYSFISGLAEPVGALIGFLILLPYMSDTIF
                     GIVFGCIAGIMVFISVDELLPSAIKYGEHHYSILGFISGMFIMSVSLILLQ"
     gene            29330..30103
                     /locus_tag="PLX22_00165"
     CDS             29330..30103
                     /locus_tag="PLX22_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT18348.1"
                     /translation="MNSNSFHEIVDDIVIITLNRPKVRNAIDRDTADELSNAFQQFDA
                     NKNLKVAILTGAGGNFCSGADLLAIAEDGHRKNRLNPNIKDNGPLGPTRMLLSKPVIA
                     AISGYAVAGGLELACWCDIRMADMTAKFGVFCRRFGVPLIDGGTQRLPRLIGLSRALD
                     LIITGREINAEEAYQFGLVNYLVTDKDIKQAALELAHKIASFPQQCMRSDRMAVYKGF
                     NMPLSEALEIEFARGTDVIRNGETLEGAKLFAQQKGNKI"
     gene            complement(30159..30689)
                     /locus_tag="PLX22_00170"
     CDS             complement(30159..30689)
                     /locus_tag="PLX22_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18349.1"
                     /translation="MKGYNSIIIVLLVYALFSGCATQQKKVGCIEGKCDEGYGVYVWE
                     DGTMYAGNFKSGKFHGQGTYTWANGMTYTGIYVDGKRHGYGVMTWPDGSSYRGEFYND
                     EITGKGKYIYADGKIYTGPMVKGKANGIGILEWPDGTRYEGEFVDNRIEGKGTLIYPD
                     GRTLKGRFINGILVNE"
     gene            complement(30743..31012)
                     /locus_tag="PLX22_00175"
     CDS             complement(30743..31012)
                     /locus_tag="PLX22_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT18350.1"
                     /translation="MKITVEKIAEDDLKKQGVLSWPIWQKEISDFDWFYDTTEQFYVL
                     EGEVEVDTVDGQKVRFGAGDFVTCPKGIQCTWHVKKPIRKHYNFK"
     gene            complement(31267..31938)
                     /locus_tag="PLX22_00180"
     CDS             complement(31267..31938)
                     /locus_tag="PLX22_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18351.1"
                     /translation="MPSARFTITPEVKRQIVKIIDDRINEVHVTKEDFSELKSIVKDI
                     AKAQKELTQAQKKTEQRVAELAEAQKELADAQKRTEIEVKSLVAGLHDTRVELGGLSR
                     SFSYAFENEAYRHLPAVLKEKYGYTIKERIVRAEIGGKEINFFARAMKDGKEVFIVGE
                     SKIRLESTWRKDVFSELEDKVNAVKAEFGDVEVVRLLVTHFAKKDFLDEAEKMGVIVV
                     QSYQW"
     gene            32264..32575
                     /gene="trxA"
                     /locus_tag="PLX22_00185"
     CDS             32264..32575
                     /gene="trxA"
                     /locus_tag="PLX22_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT18352.1"
                     /translation="MALIQEVTDATFDSSVLQTSGKVLVDFWAPWCVPCRLQTPILER
                     LVSNGINAKIVKCNTDVNHGTAQKFGISAIPTLILFDNGKEIDRMVGVQPEEVLKRRL
                     Q"
     gene            complement(32684..33445)
                     /locus_tag="PLX22_00190"
     CDS             complement(32684..33445)
                     /locus_tag="PLX22_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT18353.1"
                     /translation="MRLHNVSLWRNSTIILRNINLTVNTGDQWAVLGPNGSGKSFLMN
                     IIATLVFPSEGQVVIAGETLGQVNVWDVRKHIGIVSDYLQTHYPQETMVAEVVASGFF
                     SSMGLYQELSPAMEQKALTILHSLELVHYAQTPFGKLSYGEQKRVLIGRALVYDPDIL
                     ILDEPCNGLDMKAREEFLQQLTMLIKTGKHIIYVTHHVDEIMPWINRVLLLNNGQCMY
                     AGTKNILLNEDVISKVMGCSVGIFQHNSRIFSYVK"
     gene            complement(33506..34504)
                     /locus_tag="PLX22_00195"
     CDS             complement(33506..34504)
                     /locus_tag="PLX22_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT18354.1"
                     /translation="MRYKSIILVLFLILTACSKKEAVDTIVTINSFFGDVTITSHEGN
                     KPPAVGQVLTISDTIVTGANSVVDLLYRNSGIIRVNENTTVVVSSLIKAGSDDVVLTL
                     EKGKTFATLGKLKKGDGFAIKAKTVIAAVRGTSFRLVADEKASRVDVVSGKVMVKPVN
                     NETVIEDIEVIVEENQTVTLDTKTAATISQKIDENKVEVVAEQEQQKIIDSIKETIKP
                     TEIPKETVKEIKQEVKDIPVITTVQEEIKQEIQKVIETDKEAKQRKNEEKLKKAEEAK
                     ALKLKLQNEQEAKILAEKLAKEKAEQEKVEKEKAEQEKAAKEKKIKEDRIKNIPTL"
     gene            34952..36247
                     /locus_tag="PLX22_00200"
     CDS             34952..36247
                     /locus_tag="PLX22_00200"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT18355.1"
                     /translation="MDRITQNDTLIDTLGNCTIDSPLTIPKFISDSQRIAVDIEADII
                     EQSLEKGKIPSFENAGPRKKIFFKPQNTRAAIVTCGGLCPGINNVIQGIVRMLTFQYG
                     VKTIFGVKYGFEGLVPRFGHSFIELTPQFVHDLHEKGGTVLGSSRGRQDEKEIVDTLV
                     AHDINILFVIGGDGSLRGAYDIYREVKKRNLPIAIVGIPKTIDNDIWFVDKTFGFSTA
                     VSTAAQAIYAAHAEAIGAKNGIGIVRVMGRDAGYIAAYVTLASNEVNYCFIPEVHFDL
                     EGAHGFLPHLEKRLYAASHAVIVVAEGAGQEYFDEPKLTDVSGNIKYGDIGLLLNNTI
                     GAYCKQRNIPVTIKYIDTSYLVRSTPASSDDAVYCIMLSQNAVHGAMAGKTGFVVGSW
                     HSYYTYIPLSLVTKKRKRIDPHGYLWSIVKQSTCQPDFF"
     gene            36534..37526
                     /locus_tag="PLX22_00205"
     CDS             36534..37526
                     /locus_tag="PLX22_00205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT18356.1"
                     /translation="MTANDMDQLFKIKQLYANIGKLITSSLEIEHILEGIMEEVHTFF
                     GPDNWSLLRYDPNSEELYFVISHGIDFDSIKNIRLQKGEGVAGYVVETKKPVFIPNTS
                     HDTRFSDRIDRITGFSTKSVMAVPLIFRDTMYGVIELINKSTGGIFSSDDYLILQTIA
                     DFGAIAFANSLLYEKALTLTALDSLTGLYNNKKLNDIIASWSKDEAHIRRDDTTHVIA
                     VLNIDIKGLNEINTIAGHRDGDRVIKHISRILKTSLRENDMTFRTGGGNFISIINCDN
                     YFDMKSLVERILHKISAITVPDYPDLHIQVSHGLSIGTRNRINELIQETLEQKS"
     gene            complement(37576..38046)
                     /gene="tadA"
                     /locus_tag="PLX22_00210"
     CDS             complement(37576..38046)
                     /gene="tadA"
                     /locus_tag="PLX22_00210"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT18357.1"
                     /translation="MNNNDNNPEFYMTIALEEAKKAHDEGEIPVGAAIVFKDGVIATA
                     HNTTRQCSDPTAHAELIAIKQAAMVLKNERLNGCRMYVTKEPCAMCAGAIVHARIEKL
                     YIGVKDKRFGACGTVLNVCGNGVLNHKPQIIFGILEEECKNLLKSFFEQLRKKG"
     assembly_gap    38061..38160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38241..40112
                     /locus_tag="PLX22_00215"
     CDS             38241..40112
                     /locus_tag="PLX22_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT18358.1"
                     /translation="MMRNPLVDHRDVYFVLFEVLETDKLQQFPKFAEHDKDMYEATID
                     LALKMATEEVYPVNKEADKIGVRYDPDTKKVIIPQGYKGGLRKYNEAGFVGMIEDPEI
                     GGMGMPGAIAIAVNEIFTSASLSFTTYPGLSHGAAALIENFGTDEQKKVYLEKMLSGQ
                     WGGTMCLTEPEAGSDVGNLKTKAIPQPDGTYRIQGQKIFITSGDNDFYENIIHPVLAR
                     IEGDPPGTKGISIFIVPKYRINPDGSIGELNDVVCSGIEHKMGIHGSATCSLNFGDNG
                     TCAGYLLGQPRKGMKIMFHMMNEARLGVACQGLALSSTAYMHAVTYARNRIQGVHVTQ
                     MLNPDAQKVAIIEHPDVKRMLLWMKGYVEGMRMLNYYLAHNATLIEVLDGEAKKEAQA
                     IVEILIPIAKAGCTDSAVLITSEAIQVYGGYGYISDYPVEQYMRDAKITQIYEGTNGI
                     QSMDLTMRKILMNPEQYNYTIMKKRMSEAIANAKGIVEEKYINLVENGMQKMDEVILM
                     LKEQMAAGKFLHIFAACTPVQQAMFMLTLAWLHLWAMTLTIPKMKALVGEKKGEERDA
                     FLSENEEASYYSGKVLSSQFYLGAEFPKFFGKIDAILFNETAIIKASKDIFTGALLE"
BASE COUNT        12714 a         8383 c         6698 g        12379 t
ORIGIN      
        1 catgtccaac atggatgaga gctgaaaagc ttataagcat tgcacatcct caatttcagg
       61 atgatttaat aaagcaggcc gaaaaaatga aaatatggac acgaacaaat aaacaaatac
      121 cagtgtaaac tatttctttt catcaataca gggtcatacg ttttacccct gtattgatga
      181 aatgtctact gaaaaaatat agagacttac tatcaattta aatatcttct aatggttcca
      241 ttgttttatg caaactatgg gaattgatta attttagtca tcaatgacta cccaataatt
      301 gccatcaaac ttcgctgtca gctcgtgagg gtgtcctaat cgaataaact gaaattttac
      361 cactggctta ttttttatca tgaatgcctt tgcctcattt tcagttaaaa gttcaaaagc
      421 ccaccataca gcagtctcag caatccactc attaatatat cctttccatt tataaatatc
      481 acgctctgga catatgcccg tttcggttgg caatactatc acttttaccc atttactatt
      541 gcgtacatag ctataataaa tctctaaccc atccaggtca gtagtccatt gctcaataag
      601 ctgcccttca tggcttaaac catagaaagt attgtcactg ccccactctt caatcatagc
      661 attgacgtaa tcgttcatac gtttggtcat atcccagtcc tttttaacgt caattgtttt
      721 taaagatagc aatgtaatta tgtaattata caatcgatca tgtaaagcca ttttaaagga
      781 attagcttct attacataaa acagtaaaat atttatttac gtgccaaacg ttcaatctga
      841 taagttttat aatatcgaat atgcggatag tgtgtaatca aataatcttc aacttcagga
      901 tcaaaactat agccaacaat aaaaccagaa atatcctgtt taaaatgtga ttgcaaacga
      961 tgaagcattt tgttgaaatc atcgatatca ctttttcccg gttgagattt acattcacca
     1021 acaacaatca tagtcacatc gttttttaaa ccttttacaa agatgttgat ctcatcttta
     1081 tttttttgat cgtacttgat aaattttctt tctacttctt gtggtacaaa cccatactct
     1141 ttttcaatga aagattccat aaacggaata accttatcct ctatgccata gccaacagca
     1201 tgagacaacc cacctaattg tttttttacc atattcatat catacagcaa ttgctggaca
     1261 actacttctg tctttttctg tgcctcagcc aactcctcca tctttactgt taacgcatca
     1321 acacgctgct ccgttcgctt ctgtgcctca gccaactcct ccatcttcac tgttaacgca
     1381 tcaacacgct gctccgttcg cttctgtgcc tctacaagct cctccatctt tactgttaac
     1441 gcatcaacac gctgctctgt tcgcttctgt gcctccgcaa gctcctnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnntaat tttttgttcc gttttttcct gggcatcagc
     1621 taaacgagca acaatattct tcaaatcatt aaaatcttcc ttttttactg attccttaac
     1681 atttttatca atctcttcaa gaagttccat gattacacgt ttcactttgg gttcaagctc
     1741 atccagatgt ttataaatct ttacactata cggcatcctg atacctcttt ttactgaata
     1801 tttttatata tgctatgcaa gattttgtta cccattaact atctattcca attcaaaaat
     1861 agtaagatta tgtattttca tcaataattt tattaatttt ttttaaaagg tatcattaat
     1921 cttataactt taatgatacc tttttattga caatatatat ctattgttca gtggctcatg
     1981 tatatctacc aacaatcgca acactgatga cattcatcat tgggaatcca cctaaatttt
     2041 gcgttaatcc aatatcagga tttttaagct gccgcttatc agccctgcca tgtaattgca
     2101 ggtatatctc gtataccatc cgtaagcccg atgcaccaat agggtgccca aagcatttga
     2161 gccctccatc aatctgacag ggaattttac cggtactatc aaaaaatcca tccatcacat
     2221 catcaatagc ttttcctcgt tcagatatat gtaaatcctc catggttaca agctcagtga
     2281 tggaaaaaca gtcatgaacc tctaaaaggc tcaactcctc acgaggattt tttatgcccg
     2341 cttccgcata agcacgaatt gcagcctttt cagtattgac aacatgtgcg ccgtcccaat
     2401 tggaaaatgc catctcttcg ccattggata ccgaaatctg taacgctttt actgtcacaa
     2461 aatccttctt cttcattgcc tttgcaacat caacactgca tactatcgcg caggctgctc
     2521 catcgctaac cccgcaacag tcaaatagcc ccagaggata ggcaatcatt ggcgattgca
     2581 gtacctgctc ttccgttacc ggacgctgca gatgagcttt tggattcaga gctccattag
     2641 catgactttt aaccgagatg tgagccattg cccttttaac atcctccatc tttaaaccat
     2701 actttgctgc atatcccccc gccagcatag caaagcaacc tggtgcagta acattaggaa
     2761 accatagtcc attaagaatc cccagagcct ggctaaattc gggcaagccg ccatagccaa
     2821 tatctttgag cttttcacag ccaattgcca gcacaatatc atacgcaccc gaggcaacag
     2881 catacacagc accacgaaat gcttcggtac cgcttgcaca aaaattttca acccgtgtaa
     2941 ctgcaatgtt gggcaaccgc agcgcaattg aggcagctaa tgcactttta ccaacattga
     3001 tctcatcaaa acaggttgca acccatgctg cctgtatctc tccgggttca atacctgcat
     3061 cagcaattgc ttcattgtac gcttcaacca taagggcgtt tgcatccgca tcccagcgct
     3121 cgccaaattt tgtgcacccc attccaatta ttgcaacttt atcttttatt ccagtagcca
     3181 tatattcctc cttactccat tattggtacc gctttccaga aatatccggt aaagcctcgc
     3241 atgctgtcct gtctatggat acgaaacgac atagctactt ttgtacccac attaagtttc
     3301 tcgagctcac agtctgtaaa atcaagaagc gttcggccac caccatcaaa ctcaacaagc
     3361 ccatacagtg ccggtggttc aacagatgct gcaagcatat caccagtata cataagcact
     3421 gtgcccattt tatccgaaaa tacataatca tccatggcac ctgatttgtt acatgcagga
     3481 ttaacacaaa taggataagg aggaaactgc ggtgtaccac atgccttaca tttatacccc
     3541 acaaaaccat tcagcatctt tcggtgacgc cataatgcag taagggatgt attgggaaca
     3601 gcttcagctc gtatacttgt atccgcgtta attaattccc taaactttaa atattttgga
     3661 tacggaatat ctttcttatt tgcaatgctc cgggtaatcc ctctgggacg cttaaaactt
     3721 tcaattgcat cagttacctt aaaggcaact atctcacaac cctgtccaaa accaacaaca
     3781 agtattttat cgccaggttt tgcactatca agcgcatggc aaagcatcac cagcggatgt
     3841 gccacacctg tatcaccaac cccatcgtgc aggttagtat aaacatgtgc cgatttaatt
     3901 ttttttgcaa tacctgcatg ttcccgcgca aatggacagg gataaataac acaggcaaac
     3961 tcatttatat ctgtgtgata gttatcgcaa aacagcttta ttgcggtagg gatgatacgc
     4021 ccaaatccct catcgcgtat aaaacgttcc tcccattgat aatcaaagcg tttatcggca
     4081 ccgcggtaat gatcaacaaa atcttcatga tacgaatatg aaccaataca ttctgctata
     4141 acattttcac gtcccactac cagggctgca gcgccatcac catacataag ctccaatgtt
     4201 gatgctgctc tggtaaaccg tctttcacca gcagtaacca taaccttgtt agccgaatca
     4261 gattctacag catccagcgc agcaacaact gccgaaacgc ctgcttttaa tgaaccagcc
     4321 atatcaacgg ttcgcacatt gttcctgcaa ttgagtgctt ctgcaataac tgcggcattc
     4381 tgtctgtcgt caaatggaaa cgatgttgaa gctgcataaa cagcatcaat acttttttgt
     4441 tcaacatttt gcagtgcatc aactgctgct gccacagcca tagtaattgc atcttcgtcc
     4501 cagtttgcaa ctgaccgttc accagtcatc agtgagtata gcaaagaatt ataccatgcc
     4561 atctgctgta caacctgcat cctggataat cttcttaaag ggatgtatcc cccatacgaa
     4621 acaatcccaa tcattgaaaa agccccccat taaaatattt tttatacatt atcctgaccc
     4681 cctgcattgt ttttattatg gtagatttat agtttcaggg tttagaacca tgtgagcaaa
     4741 attttttcac ttacttaagt aatggtgcca tgtatgcaaa ggtttctcca atgttattca
     4801 atggttttgc atcacttaaa tcggttatct tactccactg cgccatgata tcctctgctg
     4861 ttatttcatc tttaccctgt ataatagtgc cactggcaca aacaattgca gcacggctat
     4921 accaaccgcc accacagtta ataagcatcc ctgtatcctt acattcatct gaacatagat
     4981 acatcaccag cggtgttaca aattccggtt ttaatttttc aaaaatctct ttaggcaata
     5041 catcttctgt catgcgggac gctgccaccg gaactatcgt gttaaccaat atattgtact
     5101 ttgcaccttc taatttcaat gcattcatca atccaacaag tcccatcttt gctgatgcat
     5161 agtttgtttg cccaaaattg ccgtataaac cggctcctga agcagtaaag atgattctgc
     5221 cccaaccctg ctcacgcatt atcttaaacg ccggtgcagt aacacaaaat gctccacgca
     5281 gatgcaccga tactacaata tcccattcat cttcggtcat cttgacaaac gttttatcac
     5341 gtaaaatgcc agcattgttt actacaatgt ccacccgtcc aaaagcatcc attgcagttt
     5401 taataatgtt ttcaccaccc tgcacagttg caacagaatc ataattagct actgccttgc
     5461 ccccagcttt ttttatctcc tccacaacag catcagcagc actatggttt gcacctgatc
     5521 catcacgcaa actgcctaaa tcattgacaa ccaccatcgc accacgtcgt gcaaactcta
     5581 aggcataggt tcgcccaatc cctgaacctg cacccgtaac aacagcaact ttgccatcaa
     5641 gcttaatact catactaccc tcctgctcat taataatatt tcaggcaaca gtacaccatc
     5701 cattgcgcag tacatccttg ccactatcat ttaacgctac gattccatat tttgtttttt
     5761 ctgcaagctt ttccattgtg taccctttaa acgtaagaac ttggccgggc aataccggtc
     5821 gggcaaaacg cacatacaat tccttaactt ttaaaggatc tctatccggt gtaagatgat
     5881 ccactgccat cttatgagca aatgccatgg tacatagccc ctgcaaaata atccccggca
     5941 acccaacctt ttttgcaaag tcatcatcaa catggatagg gttatggtct ccggaaggtt
     6001 cagcatagat ataggtctgg ccattcctta ccctcatggc atcgtcaaaa ataaattgtg
     6061 aaggtatttt accctcttca gcctttccat caggttttcc acttcctgca cttctgtcaa
     6121 aaaattccca ctgtgccctt actacaatgt catcatgctg atttttagta actgtctccc
     6181 agcctaaaat gcttcctttt tcttttaccg taatatgggt gatagttccc tcggaggtaa
     6241 cctcatcgcc gggcttaacc gcttttatcc attcaaaata ttgactgtaa tgtacaacaa
     6301 acatcatgtt tatcccaatc tcagcatcaa acattagttg tgcggttgga ccagcggcat
     6361 aaaaaacagc atacatcgga ggagctataa taccaccctg ccttctgtta tcaaagtatg
     6421 catcattgtc ctcattataa gcaagggcat aatagatact ttcatgctgt gaaattttaa
     6481 taggatctga tttatatttt ttgccaatac acgtctcatt cagcggcata cgcacctcct
     6541 gtttttatat ttaagtgaag tagatttttc ccacagtccc aattaaatgt aatacaaaca
     6601 tacatgtttt ttggtgtcaa gatattaatc tgacaaaaaa tagttatatt aatttagcta
     6661 tatagttata cattacttgt tttggcaaga attaatgata tattgttaag aaatgatcat
     6721 aataaatacg tattgactta accaacttta attatcatac catgattgaa tgcaatggtg
     6781 gtaaaaaatt tttttgataa tggctggtga gttaaaaata atttttacag gtatctatta
     6841 atgttaacta tatgccgcat aatgatgtaa aacgatatta taaccatgat caaggtatac
     6901 aaacacttaa ggtaagcaag attattactc cacttactgc tgttgttatt gcgctgtttc
     6961 tttatcagga tatttatatt ctgcattttt attttacctt ctggtacaga ttcattccaa
     7021 taatgctatg tatagtctac tttttgctaa cgctttcttt actaaaaagg catgtacact
     7081 atgttattcc gctatatacc ttacttttat tatcagggat tataatgata ctgggtattg
     7141 catatcgcat atttaccaac cctctctatc ctgaaacatt tttatcaaat gtatctacag
     7201 gcattcaaac agtatttata gtgctatttt taatagcatc aggtgcaaga agatttttac
     7261 caatagtata cactgtttct ttagtgcttt tcctgatatc tctatcattc aataaacaat
     7321 taacatcttt ggagttatca gcactttcca atcctattgc cacaactctt tttttaagtt
     7381 tattagctta cgtatttgaa agtatgaatt ccagagaatt tgcattgcgg tttactgtag
     7441 aacaaaatga ggatgaactc aatcaatata aagataccat ggaaaaagaa ttagccttcg
     7501 catcatacct gcatagatta atcatgccgc ttaaacctcc ggtaccatct atttcagtat
     7561 actatcatcc atatcataat ttaggcggtg atttttatga ctttatacca cttgataaaa
     7621 agaaagacag gattggcata tttatcagtg atgtaacagg gcatggggta tcttctgcat
     7681 ttattactgc gacactaaaa cactatttag tagttggcaa aaaatatcat gaaaatccac
     7741 aaaaattgat gtactatctt aatgaatatt taacagggaa tattggccaa aattttgtaa
     7801 cagcattata ttgtatcatt gactttaata aaaaagaatt aatatacagc aatgcagctc
     7861 attatgcacc aataatagtt gggaaagatg gcaacataca tgaactggaa aaaacaggaa
     7921 aacctttccc attagggatt ataaaaaaaa gaaaattaat aaaatatagc aaaaattttt
     7981 cttattcaac aatacagttg cagcaaggtt caaaattagt attatatacc gatggtatta
     8041 ttgaactata taatgcaggg aatccatcca attttgatac aggtgtattc tttcaattat
     8101 tacagcaata ccacctgcta tcatctgccg atatgttaaa cgaaattgac aggcatctta
     8161 ccagtcagca tgggaacaat tatattaacg atgatatatg tgtaatagtt gttgatatat
     8221 aatatcagct cattataaat tctgtttttt ctttatcaag ccattcgggc aattccagta
     8281 taaatgcttt gatctgatcg cgtacttttc tgtaatgata caatgcttcc tcctgtgttt
     8341 ttgcttcccc tgccagttga ggtggatctt caaatcctct gtgcatcact tttgttttac
     8401 caggaaacac cggacaattt tcgtatgcgt gcccacaaac cgttataaca aaatcaaact
     8461 gattattgat taactcatta acatgctttg acacatgatg tgaaatatca acacctgctt
     8521 ctttcataac ttttattgcc aaagggtcaa ctgttttggg ttcaatccct gccgaatatg
     8581 cttcaatgat atcaccgcgt aatgcattgg caaaaccttc tgccatctga ctacggcagg
     8641 agtttccggt acacagaaat aaaatggaaa tttttttatt cataacaacc tttttaaaaa
     8701 agatttttat atcatggcat atcaccattg ttatcacaac tacagaaaca gtagtataaa
     8761 ttttttgtta atttttgtta tacaatattt ttatatatac ttactaccag ttttgtaaat
     8821 ctcgtaaaag ttcaaagtaa aacacgcaaa ttcttaatta aaaatatcgt ttcttaaaca
     8881 ccagggcaac atctacaagt ccaattaata ccggaacctc aaccagagga ccaataacag
     8941 ttgcaaatgc ctgccctgag ttaattccaa aaattgcaat agcaacagct atagctaact
     9001 caaaatcatt tgaaccagcg gtaaaagcta ctgctgcagt ttttgaataa tctcccgtaa
     9061 tttttttgat aatataaaat gtaacaaacc acatcaaaac aaaatataac agcattggta
     9121 atgctgcaat gacaacatcc tgtggacgtt caatgatttt attcccttgc atagaaaaca
     9181 ttacaataat tgtaaaaagt aatgcaatca gtgttagcgg acttatcctg ggtataaatt
     9241 ttgcttcata ccactgttta cccatataag atattaaacc aaaacgcgta gcaacacctg
     9301 ctaaaaacgg tattcctaaa tagataaaaa ctgttttagc agattcagat attgatatag
     9361 aaatatcaag tccttcagca agaccaaacc aattcaaagc cagtgttata aataaataga
     9421 tatacactgc ataaaacaat acctgaaata cagcattaaa tgcaactaaa ccaactgcca
     9481 attccctgtc accacctgct aattcattcc atactattac catggcaata catctagcaa
     9541 gccctacaag aataactcca accatatatt ccggatatcc ccttaaaaga ataacggcca
     9601 ggccaaacat aacaattgga ccaacaaccc agttcagaag caatgatatt ccaagcaatc
     9661 tcttattctg gaatattttt cccatttctt catattttac ttttgcaagt ggtggataca
     9721 tcataagtat caaaccaatt gcgataggaa tgttggtagt acccgactga agtgaatata
     9781 taaatgtttt aatttctggt aaaaaatagc ctattgctac ccctgcccca attgcaaaaa
     9841 aaatccacag agttaaaaat ctgtcaacaa acgaaagctt tttagctacc ccatcagcca
     9901 taaattcacc tctttactta tattctgcaa gcccccttgt aggtacaacc attaaaaaaa
     9961 taaatctatt tattggatta actttttaat ctcttcaata gaggggactt tgccagcaat
    10021 cttaacctga ccatcaacaa caagagctgg cgttatcatc acaccatagc tcattatttg
    10081 tttaatatcg gttaccttat tcaattggta cggtatacct aattcctttg ctgcctgctc
    10141 ggttaaatgc tgaagtgtgg tacactttga acagcctgtt cctaaaattt caaatttttt
    10201 cataaccacc tcaaaaaata gactatatat ggggtcagag cctcaatact accatagcaa
    10261 tccaaacaat attccggtga tagttgccat cacaatgacc agtgaaacat atacagcagt
    10321 cttttgtgta ccaataacgc tacgaataac cagcatactg ggaagggaca atgtaggtcc
    10381 agccagaaga agtgctaacg ccggaccatg acccatacca ttacctaaaa gcccctgtaa
    10441 aatgggaact tcggtaagcg ttgcaaaata cataaaggca ccaacaactg atgagaagaa
    10501 attggcaaag aatgaattac cacccactgc catagcaatc cattgtgatg gtataatacc
    10561 ttcatgcccc ggactgccaa gtaaaaagcc tgcagccaga accccaccca aaagcaatgg
    10621 cagtatttgt ttggcaaatg accatgtttg ctccaaccat gtcaacaact cacctttttc
    10681 ggttatgccg atagctatca aagcaataat acctgcaaca aatggtatta atggcctgtg
    10741 cggaaataaa aagcctgaca ttactgtaac aatgatagca ataactatct tccaccatgt
    10801 tacagtaaac catgcccaca gcaccaatcc aaagagtagt gacacaacac cggtaatctc
    10861 ccatttataa ctgtacacat aataccagat tccctcaggt gcatctggtt taccccagtt
    10921 aacaaacacc agtatggcta ccattaaaag aaaatagata accgtctgcc acaggggacg
    10981 tgacgtttcg ggctggggca tattcatcat agcattggct ttttgtatct cttcttttcg
    11041 gaaaataaga tacatcaatc caccaatgaa aatactgaaa acaattgcac cgactgcgcg
    11101 tgcaatgcct atttcaagtc caagcacccg tgctgtcaaa atgattgcca gtacattgat
    11161 ggcaggtccc gaatacaaaa aagctgttgc aggacctaat cctgcgccca tagtataaat
    11221 acccgcaaaa agcgggagga ccgtgcatga acaaacagct aaaattgtcc ccgaaacaga
    11281 tgcgacacca tacgccataa gttttggtgc ggcaggcccc aggtacttca tgaccgaatt
    11341 ttgactaaca aatgtggcga tagctcctgc aatgaaaaac gctggtacaa ggcataacaa
    11401 gacatgttcg cgagcatacc accttgtcaa cttcagcgcc tcagatacag cattgtcaaa
    11461 acggggattg ccagcaggca aatgaaagaa aatgacaaag gctataacca tatacataca
    11521 tatttttagt tcttttttcc agtccatggc caatccttat acctggtaat tataactgtt
    11581 gcgtactgtt tatacagcaa cgagcaacct ctaactgttc tgttgcattc cgttgaatca
    11641 ccgtatcaat acagctaata aattcactaa cacagggcat cttcaaaaaa taataaacgg
    11701 ttgtgccctc ttttttatca tataccagcc ctgcactttt caataccgat aggtgtttgg
    11761 atactgtgga catatcagcg ccaatcaatt cctgcagttt gcatacacac tgtgcccctt
    11821 tttgcagctc ttctaccata tataaccgcg tgggatgtgc cagcgccttc ataacagcag
    11881 ctcgtgcctc atattttttt tgtgtcatgg tatccataat aacctcgtat tatttcttat
    11941 ttggcaattt agcaaaatag tcaataatta tcaaaaaaaa ttacagtaat aaaatatcaa
    12001 ccaatcctaa atattacatg gtatgttgaa gttctgcaaa taaatatgtt gtacactact
    12061 ttttacatgt cactaacaag ccgcattgaa gtaaatagtt tttacaactg tatgctgaaa
    12121 agaatgatag tggcacgccg attaaaatat tgacaattga tagttttaaa atgtaaatag
    12181 taattgttaa ttacatgcat aacgatgcac acaaaaaagt tcttgaatat ggcatgttaa
    12241 agtataataa atgtaaaccg taattatgat cttcaacaat agtttacata ctataaccat
    12301 ggagtgtaat tacacaataa tacacgatag caggtgcata tggataaaaa tactatacgt
    12361 acagcttcac aattagcttt taccataacc aataccatta tcaaacactt tggagcgcgt
    12421 ccttcatgct ctgaacagtc aaacaatgct gcaaaagtac tgcagtttat gtattcaata
    12481 cattgtgata gctctcaact tgaaacattc actacccatc ccagtgcgtt tttaggcttc
    12541 cttaccctgg tgccatggat ttatatagcc tgccttattt ttattatttt acactgttat
    12601 atcattgctg cagttgggtt taccatttcc attatcattg ctacatcaca attcattttt
    12661 tataaaggaa catttgattt tttatataca caaaaagagg gcactaacgc tattggtagt
    12721 attgaaccgt caaacgatgt acgccagcag attataatca gcggacatca tgacagtgcc
    12781 tatgaattca gatacatgcg caccacaccg cgcctgtacc gcatacgtgt tgcttccatt
    12841 atactttcaa tactactatc ctttttgctt ggctggctca tcgttgtcaa tgcgtacacc
    12901 tttgattccg ccactgcaca tactgttctc ctaacctttc ttataattct gggcattgct
    12961 aatatgcaga tgcttttttt taaaagcaaa cacgcaactc ccggagccgg agacaactta
    13021 atcgcaagta ccattactgt agcactggca aacatttttg caaactatcg ccccaactat
    13081 acccgcataa tttttgcaag ctttgatgct gaggaaagcg ggctcaaggg ctcaaaagca
    13141 tttgccacca gtcacaaaca tgaaattggc agcatgccaa catttcattt caacatggac
    13201 agcctctata aggttgattt aatacaattt ttaacaagcg atgtaaacgg cttcgttcct
    13261 ctttcaaaag aacttgcaag gcaatgtgtg gcgatagctc atgctatggg ctaccctgcc
    13321 aaaacatttg ccatgtaccc cggaacaggt gcaaccgatg cagcacctgt tgcacaagct
    13381 ggcgcaatag caacaacgct cattgcactc ccaacggagg ttgaaaaaca acacagcgtg
    13441 tatcacacca tggacgacac caccagcaat atacagcctg aagttgttga agcagcaata
    13501 gcaattgctg tgaagcttgt tcactatatt gacaatgaaa tacgcggata caggtgggga
    13561 aatcgcgcca gacaggactc gaacctgtgg cccacggctt agaaggccgt tgctctatcc
    13621 agctgagcta ctggcgcaca tagtagtaca ttatatattc ttagactata ttttaaaaat
    13681 tcggggcgag aggatttgaa cctccgacct catggtccca aaccacgcgc tctagccgga
    13741 ctgagccacg ccccggaatg acagtaaaaa aaattatatc gcacacaacg attttgtcaa
    13801 agtaataaat agtatagttg tgtcaatatt tttttattcc ccttctacgg gataatcctg
    13861 caaaactttt tttaacagtt caatgtcatc cgtaatgata ccatcaacct ctatatccag
    13921 cagccgtcgc atatcacgtt cgctattcac tgtccacaca tcaacctgta tgcccctttc
    13981 atgcgccatg cgtaccagtg taccatttgc cagatgtgaa ggtccaataa actccggaat
    14041 ttgcaacgca tcgccggaaa actgtttaaa taatcctaac aatcctgaac ggaataaaaa
    14101 ataaaaacca agaacacccc atagcccaaa tgatgttgcc ataccaggta aacgcttgcg
    14161 aatatatgca aggttttttc catgctgcga agcccctacc accctgtgtt gagcattgtg
    14221 cttttcaatc acatcacaaa atttttcagc cagcagaatg ttgttatctt tcaggtcaac
    14281 attaaaccgc tgatgaggaa atgtctccag catctcctca agcgtcatta tggttatacc
    14341 tttccccctg aatggataag tttccccatc ctgtgtaaaa ttgtatccag catcaagctg
    14401 tttaatctgc aacaggggaa gctcaccaat ctttccgtta ccatctgtta tgttcgatag
    14461 ttcctcatca tgagctatca ccacataacc atcctttgtt atgtgagcat ctgtttcaat
    14521 gacatcagct cccagctcta ttgctttttc aaacgccaat gctgtgtttt cgggatattc
    14581 ccttgaattt cccctgtgag caaagatacg agcaggttta tcaaaaaaag gttttcccat
    14641 aattgttaac cctcaatttt tgcgactttg catgttatcc atgaccttga tgctgatacc
    14701 ttcagccaga atacactgtt tgatactcct cagaaaatag tattatttaa ccatgtcatt
    14761 ccgggcccgg tccgaaatct cttttaccac taccgtcatt ccgaacattg tccggaatcc
    14821 gaataattaa cgttagattc tgaaaccagt tcgggatatg attcaaaatg acatgcccat
    14881 cccacggcga taccccttaa ttcaacttcg cacggcacag gcggcttcgc ctcagaatga
    14941 cgacaacaat ccattttcat cccttacagg tggaaatgaa ccagccgtcg gcaacgcctc
    15001 aataaaataa tactacaatt aatactacaa tccctgttat tcatatttac tgtatcttta
    15061 aaaaccctgt caacattttt acttaccaga cagtagcaaa aaaacataca gtattttact
    15121 tgatgaaaat aaagtaatat gtataataaa ctcatattaa tttgaatttt caaacaaata
    15181 gatgaagcat ttatgaattg acatacatag aacatggagg tacaggggag tattgaatga
    15241 aatatggcaa aacaatcttt attattatag caatactatc aaccattttt ataattggtg
    15301 aaattggtac ttacatctat gtagaagctc ccctgtctac agatttttat tcatcaatcc
    15361 caaaaaagga tgtgtataag cttcaaaacc actatggcat taaggttgtt accggtaaca
    15421 actggattca cttagggtgg attgctaatc ccgatgaaga atactataca atagaactta
    15481 agaatgttca tggcactgtt aaaaaagttg gaacgacaca atttggttca tttttatttc
    15541 atggtaaagg agcaacttcc gggtaataag gataaataaa aaaacaaagc atcatataga
    15601 gcttggcaac attcaggttt ttgcttccag caccaaggat ggtatgtgtg ttccatttat
    15661 taccgataac tatcgcacac tattcattcc agaacattcc gggtattata taaatgatca
    15721 tactatattt caggatgcat acggcaattg gcagcttatt ggaattacct caaaaactga
    15781 cggcaatcct gatgcagaaa aatattttgc cgttgcttca agtaaaacat ttccacccaa
    15841 tgatataatg aaggaagaat caccggttgc tgatgttggc gaattagcat gggcaccgca
    15901 tgttataaaa cacaaaaaca tctaccatat gttctggtca ccacacaaac ttcatcatat
    15961 gacctcaacc aatggcatac gatggggtaa tcatgacgta attatggaaa agccgtatca
    16021 taaattcttc agagattgca tgatactaca ggtggctaaa gaccagtggt tgctatacac
    16081 cactgcccgt ggtacatttt tttctcagat tgatgtatat caatcattca acctaaccca
    16141 atggcaatat ataaggactg cgctgcaaag tagttggggt agtgagcgca attcaccagc
    16201 attgattaca acattaaaag cgcatgcacc cgaatggatt tatgttccac aaacaaaaca
    16261 atggtacata acaacctgcg gatggaaatg ggttgcaacg cttacaaacg gtgaagctgc
    16321 aatagcaccg gtgtcatgga aatgtaaaaa ataaaacaca ctctaccagc gtagctgtgc
    16381 aacaaggcag tgatataaaa tcttaagttg cagtgttaac ggcaaatcaa gactttcttt
    16441 tacaggcttt gccacaaatg tatatgttac tggaactttg tttacattgc cgttatcatc
    16501 ataagcgcta actgctgcaa cataggtatt cccttcaaca aatggtttgc catcccaatt
    16561 attttttgca aagaacgaag tattgctggt tacaccaatt ttttttaatg catccttgtt
    16621 gccacaataa atattgtatc ctgctgcgcc atcaaccttt tcaaagttta tatatactcc
    16681 gccattatta tataacgcca ccactccttt tggtgactgc agcttttgtt tttgctgaac
    16741 cggtgtcact tcattcatgg ctacaataaa tggctgatga gcaaatgatt gcagttgagc
    16801 ctttacactt tgctgcaaag cccccctgcc atgagctatc gccaaactaa accgttgtgt
    16861 tgttccacta taggtacatg cccctgaatg gtattgatcc ccgttaagaa ttgctgattt
    16921 aaatccctgt ccattacccc atgttggctg gaagtattgc ctgccaaaaa aagtaccaaa
    16981 cggattaagg ctaactgtaa aacctttaac catcccataa tcaatcttca gcggacaaaa
    17041 agcaaaattt gaaagcacct cattatcctt tgccactgca ataaattctt tactgttggt
    17101 tactgcaaca tagccggcgg taatgtgatt gttcacattg gcaagccgta aattttcttt
    17161 tgaatgttta aaataatcaa ttttgtaagt atcctcaaca ccaaaataat ttttcttgat
    17221 gacagtaaac ggtatatctt ttgtggctgt catcgctgca aacagcggac agggtataac
    17281 ctgctgccac cgctcatcat agatgcgagc aagcgccggc atacccggtt taaagatagt
    17341 ggtgcgggga gtatccggat aggttattgt accctcaaca aacagcatgt caatgtcatc
    17401 aacaagatat atggtatagt caaaatatcc ccttgttaca ttttcaggca gagctatctc
    17461 gccacgcagt gccactgcag ccaccccatt tttgccatca ctcaatactg aagcggtaat
    17521 cttttccggg cgatagtcct ggccattgta ggcaatccat gggattaaac tcattttgtt
    17581 aagtatggta ctgccatgag cctcaacgcc ctgaataaat ccattgtcaa ccgttaccgt
    17641 tatggtatta ttttttatca tggtatcggt agcttcagca gcatagtgtt ttgtacccgg
    17701 ttgtgtgcgc acaatatgat agacaccgct atggagaggt tgcctgccaa taaagattgt
    17761 agacacacca ttgctggaaa acatattaac aggggtaagc atatcttttg actgcatgta
    17821 ataggtatat ccctgcggta actccattgg cagtttaaaa acaaagggag cagtatcctg
    17881 ctgtaacagc agcagtgatg taacatactc ataaccatcg tttgtgttta ttgcctgttt
    17941 tgctataatc ccttttgcac ctttttttat atcagccacc accggttttg ttaatgattc
    18001 catggaatgg gtgattgttt caacgacgtg ctctctgttt tttgccaaaa atggtgttgc
    18061 cataccaaag tttgttgttg aaagcaatcg catgcgtttt tcaaacgatc tttttaatgc
    18121 tgtcgttata tctccgggtg ctgattgtcc aataaatgaa taatatgcct cagccaattt
    18181 atgaatattt ctgtcctttt caacagcagt ccagtaatca ctgcaatatt ttttctctgc
    18241 ccatgaaaca tacccattaa agcttccatc ggccgtatcc tgcccaaagc tgatttcttt
    18301 caccggttta tgatttttta cataatcacc tattgtcgta aacgtaacaa aatccagaga
    18361 tgcaatatcg tcaataagct gtgacaggcc gcccgtatta gggagccatt ttagatatga
    18421 cggcaggtca tatccatacc agtacgaatc atcagcatcg gcattgatac atatcataac
    18481 gtcattattt attgttcctc tcaactgctg ataccgtaac atatgcaccc aattgtatat
    18541 gctaacattt tcaattaagt cagcctgatt gtatgcgggt atcaccatca ttttttcatg
    18601 agtatttttg ttatgataca taagcggatt atgagcttct tcaagcgata gttcatctac
    18661 aaaaactcta aatgcatcaa atgggatgcc actatagtat aaaacaattc cctgcaagcc
    18721 cagctgttta tagagctcat aattcccgga tgtagccatc atctcctgag ggcgaacaaa
    18781 aggtgagtag tccttaaata tatctttgac gccacttctt ctggaatttg aaatagcata
    18841 tttgactgaa tacatacatt catcctgtgt cattgctgat accagaccat tattatacga
    18901 catcaggatt atttcatcac catgttcctt cacacggcgt tttatgtttg caataatatc
    18961 gggagcatac tgaggcaaaa tttcttccag tgtaaaaagg ttttcaaaat cccatacacc
    19021 ttttaccgga atcccttttt tatttttttc atccagcact tctatgatct ttcgtataac
    19081 gcgtatatct ttcccaaatc ccgcctcatc attggtatct atcctgtatg aatgatacat
    19141 gttcacatga aatcccagcg aaacatagac gttatgattg gagtgtttgc caatagtcaa
    19201 aagttttgga ataaccatat aaccacaggc atagacgaag acaaccagaa gtatgccata
    19261 acaaacatac cgaataatct ttttcatact gtcctccaca ataaaaatga tagcatctga
    19321 accaacgcta acatcgctaa ccttcaatgc agaaagttaa gtcattccca ttatttatga
    19381 ttttttccat ttattcatca caatacctga tgaataccgt tacgctcata cctgccattc
    19441 atacatcata aggattattg taacatcatg ctataaaaat acaactaaaa tagtgaaagt
    19501 attaatgtac actaacccgt ttaattttat attgatttta atgtttaaac acactacaat
    19561 attccgggac agtgtaaaat ggtcatttca caatggcaaa attatatacc atgcatcagg
    19621 tgtaatccta ttatgggcat aaactattgg tgtaaggggc tctcatccat tatttactgt
    19681 acaatacaaa cctttttcaa tgtgcccatg ctataaaacg tttttaaaag gattaaatat
    19741 gaaaaaacaa ctaacagtaa tgacattgtt tcatgttctt atttttttct tcagcatatc
    19801 ctgcagcagc aaaactgaaa accatgtaac acctattgtg caaaatattt tatacacaaa
    19861 aactgccata gaagattatt tgaaaaacag aataaaccag caatcgtcac ctatactatt
    19921 tgccagtttc tgggattttg acggcacaat aataaaaggt gattgttctg aaggattaca
    19981 agaagaaaac aaacaagtct ataaaggact ggtacaggta gccatagaaa ataatttttc
    20041 aaaaaaatat aaaaataatg aatacaataa atttttacaa cactacgcat atcttgaaac
    20101 acacaaggga cacagcgatg catatatata tctggcaaca ttatttaccg ggagggatat
    20161 ccgcgatcta cagcaattat cgcaacacta tttttcacat actcttcgtt actatttttt
    20221 tgcatcatca atagcaataa tgaatcaact taaagcacat ggaatcaaaa tatacattat
    20281 ttcagcaagt cctgactatt ttgtaaaagg tgctgcatac tcattaaccg tatcagcaga
    20341 ccacatctat ggaataaaaa ttaaaaccaa taatggaata gcaacagaac aggtgatacc
    20401 accacttacc tatgctgaag gaaaaacaaa attgctaaaa tcaatcattg acagattaaa
    20461 aaaagaaaat aaaacaaatc atgtgtatgc aatagctgcg tttggcaaca gctatcatac
    20521 cgacggtaat tttttaaact atgttgcaac gcaaacactc cctgctggta aaccggttag
    20581 cgtgatgatc aatgggggta gcccgcccaa aaaataccat gcattatttg aatgcgtcat
    20641 acaaaatgat gtaatagcaa aataacttta tgaaacggta tgccatattt tttttcataa
    20701 ttttatttgg tttattgcct ctttgtacac cggcacaaaa tcaatggggg tattattgta
    20761 aggacacata cagcaataac tccaaacact actgggataa cacactcaaa agcctttcag
    20821 tactgtgttt tactggaatc actatttcta aaaacgcatg taacatccct tcatttagca
    20881 atgagcacta tctttttact aaagcacaac aatataatat tgcaattata cctcatgtaa
    20941 cattcacaag tgtaaccgag gggatgtcat ttttaaaagc ccgagagtgg gataatgtca
    21001 ttacaacact tgcaaatgca ttacacacaa atgcatgggc aggtattcat tttgactttg
    21061 aatatctacc tgcagcatac agcaatgctt atgcacagtt tttaaaaaaa tttagatcga
    21121 tagctcccga tataaaactt tcagcagcac tttttccaca gattgagttt cataaagaac
    21181 atgcagcatt ccacaattat gaaaaactgt ctccattctt gaatgaagtt gtcattatgt
    21241 gttacgatta tcacaatcct aaaacaaagc ctggaccggt aactgatatg caatggacca
    21301 tgaaaaatat tgaatatgca ttacggtttt ttaaagcaca tcagatatgg ttaggcatgc
    21361 cggcttatgg atatatgtgg aaaaatggga aataccattc agttattact acacgtgcaa
    21421 ttccaaaata tacatcaatc tacagtaact atcgccacag ctccggaaca ctgtgtattg
    21481 aatatataaa aaatgatgat atgttcattg ccctggtatc ggataccgta actataaaaa
    21541 aactacagca ccttgcaaca gtatattcat taaagggaac tgctttatgg cgtttgggct
    21601 ttgaataaaa aaggggcatc cctgcccctt aatcgtggag tatctggagg gttaacactt
    21661 agaatacaac ttcggtctta acatagaacg tagtttccgg atcttccttt gcatcattgt
    21721 ctgcatcttg atagtatgcc atcacacgga tgtttttgtt gattgcatac cctaatcctg
    21781 ctgcccagat ggttacttca gaatcatcaa catcttcgtt ttcaccatat gaataccgcc
    21841 cggtgattaa gattgggata ttgataacgc tttccagatt ggcaagcaac cagctatcaa
    21901 ccaaggtata ttcattttca acgcctcctt tcttgacctc gggcataaca tacatagcgc
    21961 caaccttgac caaatcagtt gaatatgatg caccaaatgc catgtagcgg ctataattgt
    22021 ccgaaccatg accatcatca gttatttgat ttcggtaggt tccaaatata tcaagccctt
    22081 tcacaggagt aatggttagc ataccgtaat atgctttccc atcatcgccc aattcatcct
    22141 cgtttacctt cttgtatcct tcgccgttta ccagagatcc tgacagctta accattttaa
    22201 agaaagaaag atcaacactt acacccatat cggcggaact gtctattgat gtaccaatga
    22261 tgtttttagc ctgatcaaca taattctgct caacccatcg gtaatcactc ttgtcatcaa
    22321 taagcccaat aagtggagta ccaatcatac caaacttaaa atttgcatca acaaaatcca
    22381 ttttttgttt agcctgtata taggcatatt ttacatatac cgtatacgga tcgctttttg
    22441 ctttaacatc tactgtttca ggatttcctg actgggtgtc tgtaacttca atttcatctt
    22501 ctgactctac ctgcccaaca tcggttgtaa cccgtgcgct ccacacacta tcaaattcct
    22561 ttttaaaatc aaggtaaaca cgcttgattt caaatgtgtt ctgatgctcg gtagtatcat
    22621 cgtaattgct cagttcctta ttccactcta aatacgcttt ccctgaaatt ttaatatcct
    22681 taatctcttc ttttacagcc tcctgagcaa acgctgccgt agcaaacgtc atcatagcca
    22741 tatacacaaa accctttaaa aacttcatgt aacaacctcc ttaactaata gttaattgta
    22801 ttgattttta aaaacaatct gagggtaaga aagcagtctg atataaaata gatttgaagg
    22861 atatgttaat atttagttaa atttcatttt ttttactgct gtcgcttttt ttattgccgg
    22921 taatcgtgct ctgtcacagg gcaacggcat tcttccaggc agcaattatg tacgttgaaa
    22981 aaatcccggc tgtacactta agtttttaag ggtgaaggct tattaccgat aactatcgcc
    23041 aaaataataa ctacaatatt aaaatgatta tattatttta ttgacttttt tattgttttc
    23101 actatattta gataggatac taatggccat gaaattgttt ttttagcatg gttttttatt
    23161 tacagaaaaa cttcaagaga tacgggcatg aaacctaaca atctaacagc ggaacaatac
    23221 tctattttca aagaaaatgg caccgaacca ccatttaaca atgcgtattg gaacaaaaaa
    23281 gagcatggca tttatgttga catagtatca ggagaagtgc tattttcatc gcttgataag
    23341 tttgactcag gtaccggctg gccaagtttt acaaaaccag taaacaacaa tgctgttatc
    23401 acatgctctg acccctcaca tggaatgata cgcaccgaag tgcgcagcaa aagctcagat
    23461 ggccatttag ggcatgtatt tgccgatggt cctgctcctg cgggattgcg gtattgcatc
    23521 aattctgcct cattgcgatt catcccggta aaaaaaatta tagaagaagg gtataacgat
    23581 tatatctacc tctttgagaa tacgcctata gggtttgaac agataataat tggcggcggt
    23641 tgtttctggg gtgttgaagc atactttaag aaagtaaagg gcatacttga taccacagtt
    23701 gggtattcgg gtggaacaat taaaaatccc gattatacca tggtgtgtac gggtaccacc
    23761 ggtcatgcag aaacggtgct cattacattt aatccagata ttgtaaatct tgaaagaata
    23821 ctaaaacatt ttttttcaat tcataatcct tctacgctca accgccaggg ccctgatata
    23881 ggcacacaat atcgttcagc gttatactat atcaacgata atcaaaaaat tactattgat
    23941 accattattc agcaaattac atccaggggg gcgatagtta ctactgaagt agctaaagct
    24001 ataaattttt ttaaagccga agaataccat caagattatt tagataaaaa tccaggcggg
    24061 tattgtcata ttaatctgaa taagatttat caggtttaag gttattcctg ataaacaatg
    24121 acatggatta ctgattcgct ggatgcaaca ttttctattt tgtgtggtat gagcgataga
    24181 aaatagatac tgtcaccatg gtttaaaata acttcatttg aaccaagagt tattttaatg
    24241 gaaccttttt taacatggat aaattcttca ccattgtgga tggaaaaatc cttctttgta
    24301 taattccctt ccagtgtaat aatgtatgct ttaacagcaa ctacctcatt aacatcagct
    24361 tcatcggcag tatgcgatgg ttcaacattg ttcaaaaaaa tatttttagg cctttcattt
    24421 tttaactgta taacatcatc cttttttatg accgtatatc cccattcaac aggggtgccc
    24481 ggggctatta cctttgcctg ttgcagtgac cgtgtaagct gatgaattac tcgcaaggaa
    24541 ggatacgctc tgcgttcttc tattttctgt aacaactcca cacatatgcc agttatagcg
    24601 gccagctgtt cataactcag attattgtgt tgcctgaaaa agcgtaattt atcgcttaac
    24661 gaatcgacat gagatgctga tggtttatga aaatattctg taaagcgctg gaaaacgtcg
    24721 ttgttatctg ttttgggaat atcgtatgat gaaaaagagc tcatggaatg ttccttaaca
    24781 caattattat atattcaact gcaaaacaac ctctcgttat gatccttacc atatgcacca
    24841 ccacacgaac atgcatcaat tccgctgcta taacatcaac ttctgataat taatatatcc
    24901 tcataattat ttttatcata taaaatattc ttttacaaca aaaaattttt gacaaaattt
    24961 taaatgccgt ttaatgtatc aatattatga aaattccctt ctccatttta tcgataacgg
    25021 aaaaagcact gacaaaatcc atggatgtcc acaccatgga gcgcattgca aaaagtatta
    25081 tacctcatta cgatctccac aaaatcatgg gcttttcaga aagtatgcca atccccaata
    25141 aagatgccgc atcccagatt gtgcacgatt tcagcgaatc aaaatatttt tttaagttag
    25201 tgtgcgccct gatggaggca caggaatacg gcatcatggg acgcaggtac cctatagctc
    25261 atctgcacga aatagttaca gaaataatca atttgggttt tatctttgat aaaaataacc
    25321 acatttttat tgaaaacgca aacaccagaa aaactcaaca ttggggtgtt ttagaagaaa
    25381 atgaagaata catcatcacg ttgctcagaa tggacattgc agcaaataca cagcttgtaa
    25441 gaaaatatac accaccgtta attgctcata cctatcaaaa cattcaaacc attgtaacgt
    25501 ctgctgttga gcagcgtaat ggcaggatat ggaactggga aggtgatggc tgcatagctg
    25561 cttttattct gggcaataaa aatatgactg ccgtttttgc tgctatagag atattacacg
    25621 aacttttttt atttaacgag cttgtatcaa aactgcctga agcagtcaat gccagaattg
    25681 caattgatac cggttattgt gcttacaaac attcttcgga agaattaaaa aaaactgaac
    25741 tggtaaaaaa aatcatggaa atagaatcac actatacgcc aatcaatgca attaccataa
    25801 gcaacacaat atatgcatca ttacatcccg atattgcaaa actttttatt ttaaccagga
    25861 agagcgccac accttattat actcacacag taaaatggga agatatatga atatcactgt
    25921 actctatcat aaaaaaaatg catcatcgtt acaaaagact tttgggacat tgatgaaatc
    25981 aaaaaaatat tctattgaat tcattgatcg tgatgaatac aataaaccaa aaaaaaccac
    26041 tgcaaattca ttatactatt tagatattac cgggctacaa caagctgaca ttcagaaata
    26101 tataaaatat tttaataaag aatcaatgtt ttatggaata atagattaca aaaatacgtt
    26161 cagcgatatt gcatggtttt ttcacaataa tgcatgtgat tatataacaa aagaaacctg
    26221 taacaatcca atttcagaaa gtcgtatacg ggcgatagtt acctttaaat caattacatt
    26281 atcagaaaca ccgcaaaaaa aatacattat tgccagagat tcatggaaaa gcatcaaacc
    26341 agaacaggaa tacaccttct gttttctttt aattgaaatt cttgatgtgc atgaattgaa
    26401 aaaacatatt gcatctgagc atctggatag taaaattttt tcatttcgtc attttgttga
    26461 aaatacactg aaccaactgg acggcaaact atggatgtgg atggatgcta cgggtttatt
    26521 tattttcccc tttgatggga aatcactccc tgctgtcgat agtgcaattc gtcttacggt
    26581 caatcgcagt attatatcgc ttgagcacac actctttaat caaataattc attacaggat
    26641 agccatgcat attggtaata ccgtatatca accacatggc aaaacaggta caataatatc
    26701 agattgcatt aattcattgt ttcacttagg gcaaaaatac actgctgatg aaggactatt
    26761 cattactgag gatgtatata atgttcttaa ttcaagaaca aagggcatgt ttatacaaac
    26821 acatcttttt gaagggcgac agatatatcg atgcaaaaac attatctaaa taagttatat
    26881 agccttcaat tcatattggc gcttgtcagc ctcaggatgt tggcggtaga taataagcac
    26941 attaccaatc atggtaatat aattacttcc tgttttttca acaatctgcc gggctaattc
    27001 tttgcgctct tctttgcaat cgataaactt tatcttaata agctcatggc acaataacac
    27061 ttcatcaatt gcattgagta cagccttaga aagaccattt ttacctatca acacaaccgg
    27121 atctaagcca tgtgcacagg ctttaagata tttaactttt ctgcggtgaa tgttattcat
    27181 acaaccctca aaataatgaa aaaattaaaa ttgctctatc acacattctt gccgcacata
    27241 ccttatggta ttttaaaact gcttgttttt ccaatccata acttgcaaaa acagttttat
    27301 cattttcttg tgtccataga ggtattacaa tttaaagttt tgaatatgcc ttatttttta
    27361 gtaattgaca ttacattgtc agtataataa caattaatgt ctatacaatt aatgatagta
    27421 ttattgcaat aattttttta ttaattgtta cacggtggtt gcaaatgcag aataattctg
    27481 cgaagtgagt tggcaatggt tggcatccgg tcatccatac ggtatgaaaa taactgacga
    27541 tagttatcct gaaccctgat tcgggatttt ggataattat ttagattaca gcaggggcgc
    27601 attataatgc gctaaaataa caaaatctat tttcaggagg aataataaca atgagtaacg
    27661 atactatctt aacaaaagac cctggtttcc gataccacga aaaagcacca aagattgatg
    27721 ctaaagaatt tcaaaaagtg attgattcca gacgatcggt tcgcatctat gaaaaggaac
    27781 caattcctga taaaataatt caacaatgcc ttcgcaatgc actgctggca ccaaattcat
    27841 caaaccttca gccatgggag atatatcaca ttgctacccc agaagtacgg cagcagatga
    27901 acaaggcatg ccttgaacag atagcagcaa aaagcgctgc agcattgttt gtggcagttg
    27961 cacgaacaaa tacatggaaa gagcatgcca aagagatggt acaacttttt gaggcagccg
    28021 ggaatgtgcc aaaatcagcc attgcctatt ataaaaagct ggtaccgttt gtttacaatc
    28081 aaggattatt tggaatatgg ggattgataa aaaggatact ctttttcatt cggggattaa
    28141 agactcctgt catccatgaa ccagtttcac actgccatat gaaaatatgg gcagtgaagt
    28201 caacagcgct ggcatgtcaa aaccttatgc tttcactacg tgcgtatgga tatgattcat
    28261 gccccatgga aggatatgat tcaaagctca taaaaaaaat attacaatta ccagaggacg
    28321 ctgttgttgt catggtaatc tctgccggta agcgtgcaat cggcgggata tatggtcccc
    28381 gtatcagata tgatgaaaac agatttttaa aaagagtgta atatactatg ctacacaatc
    28441 ccaatgttgt atttgccttc attttaacat tgttagcagg acttgcaact ggtataggga
    28501 gtgcaatctc aatcatcagc aaaaaaacca atacaaaggt actatcgctt gcattgggat
    28561 tttcggctgg tgtaatgatc tatgtttcca tgatggagat actttcaaaa tcatttgatg
    28621 tattgtacca tgaatatgga aagtcaggag gctggtttgc attaatatcc tttttcggtg
    28681 gcatagcatt aacaggaata attgatatta ttgttccatc ggcagaaaat ccgcatgaga
    28741 taaagcgtgt tgaaactatg cctctgccag caacaaaccg ggagcagcag cttatgaggc
    28801 ttgggctttt atcagccctt gctattggca tacacaattt tccagaaggg ctggctacat
    28861 ttgttgctgc tctccatgat acaaaggttg ggattcctat tgcaattgct attgcgctgc
    28921 ataacatccc tgaaggtatt gctgttgctg tacctatatt ccatgcaacc aagagcagaa
    28981 gaaaagcttt tgtatattct tttatttccg gattagcaga acctgttgga gcgttaatag
    29041 gttttttaat cctattaccc tacatgtccg acaccatctt tgggattgta tttggatgta
    29101 ttgcaggcat tatggtgttc atatcagtgg atgaattatt accatcagca ataaaatatg
    29161 gtgagcacca ttattcaatt ttaggattta tctctggcat gtttatcatg tcggtatcgc
    29221 ttatactatt acaataaatt ttatctttgc actacactat taatttgaca acaagtagtt
    29281 ttttttgtaa tgtaatagtt tcaaaattaa acaatgaaag gattagataa tgaatagtaa
    29341 tagtttccat gagatagttg atgatattgt gataatcacc ctaaacaggc caaaagttcg
    29401 taatgccatt gaccgtgata ctgccgatga gttgagtaat gcttttcaac aatttgatgc
    29461 caacaaaaat ttaaaagttg caatcttaac cggagccggg ggcaatttct gttccggtgc
    29521 tgatctactg gcaattgctg aggatggaca ccgtaaaaac aggcttaatc caaacatcaa
    29581 ggataatggg cctcttggac caacgcgcat gttgctttca aagcctgtta ttgcagcaat
    29641 atcagggtat gcagtggcag gggggctgga attagcctgc tggtgcgata ttcgcatggc
    29701 tgatatgact gctaaatttg gtgtgttttg cagacgattt ggcgttccac ttattgacgg
    29761 aggtactcaa cggttgcccc gcttaattgg attaagcaga gcattagatt taattattac
    29821 tggcagggaa attaatgcag aagaagcata tcagtttgga ttggtcaact atctcgttac
    29881 agataaagat ataaaacagg cagcactgga attggctcac aagattgctt ccttcccgca
    29941 acaatgtatg cgaagtgaca ggatggcagt ttataagggt tttaatatgc cattatctga
    30001 agctctggaa atagaatttg ccagaggaac tgatgtcata cgcaacggtg aaacattgga
    30061 aggtgcaaaa ttatttgcac agcaaaaagg aaataagatt taactattga tgaatgataa
    30121 attgccgttg tggtacacta attttttaca aagctacact attcattaac taaaatgcca
    30181 ttaataaaac gtccctttaa tgtacggcca tcaggataga taagagttcc tttgccctca
    30241 atcctgttat cgacaaactc accttcatat cgtgtaccat caggccattc caatatacca
    30301 ataccattag ctttaccctt taccattggg cctgtgtata tcttaccgtc agcatatata
    30361 tatttccctt ttcctgtaat ctcatcgttg taaaattctc ctctgtaaga actaccatca
    30421 ggccatgtca taacaccata gccatgcctt ttgccatcaa cataaattcc cgtgtatgtc
    30481 ataccatttg cccatgtata ggtaccctgc ccatggaact tgcctgattt aaagttacca
    30541 gcatacattg taccatcttc ccagacatac acaccatacc cctcatcaca cttaccttca
    30601 atacatccca cttttttttg ctgtgttgca caacctgaaa aaagagcgta tacaagtaaa
    30661 acaataatta tactgttgta gcctttcatt ttccaacctc catcaaataa aaaatacttt
    30721 ccaaaaaata tgacaatcaa cactatttaa aattatagtg ctttcgtatt ggttttttca
    30781 catgccatgt gcattgtata ccttttggac aggtaacaaa atcaccagca ccaaatcgca
    30841 ccttttgccc atcaacggta tcaacctcaa cttcaccctc aagaacataa aattgctcgg
    30901 ttgtatcata aaaccaatca aaatcggata tctccttctg ccatattggc caagacaata
    30961 cgccttgttt tttaaggtca tcttctgcta ttttttcaac agtaattttc atgggcattc
    31021 ttctccaaaa taaaattatg cgtcaatatc attccgggct tgacctataa tcaaaataat
    31081 tatcataaat cctgaaacct gagcatcata tgattcagaa tgacacgtcc agcattgtca
    31141 ttctgaggga tgcaattcca aagaatttgt ttgtttataa agaagattct tcgctattgc
    31201 tcaggatgac attgcattaa tactaagatc atttactgtt acatcgtatc ttgctatcac
    31261 cgataatcac cactgataac tttgcacaac aataacaccc atcttttcag cttcatccaa
    31321 aaaatctttt ttggcaaaat gggtaaccag taatcgtaca acctcaacat caccaaattc
    31381 agcttttaca gcattcacct tatcttcaag ttcagaaaaa acatctttcc gccaggtaga
    31441 ttctaaccgt atttttgatt cacctactat aaaaacctct ttaccgtctt tcatagctct
    31501 ggcaaaaaaa tttatttctt ttccacctat ttctgctctt actattcgct ctttgatagt
    31561 atatccatac ttttccttca gtaccgcagg caaatgccta tatgcttcat tttcaaatgc
    31621 atagctaaaa cttcttgata accctcctaa ttctactctt gtatcatgta atcctgctac
    31681 aagactttta acttctattt cagtacgttt ttgagcatca gcaagctctt tttgagcttc
    31741 agctaactct gctactcgtt gttcagtctt cttctgtgct tgtgttaact ctttttgggc
    31801 ttttgcaata tccttaacaa tagattttaa ctctgaaaaa tcttcttttg ttacatgaac
    31861 ctcattgatg cgatcatcaa taattttaac aatctgcctc ttgacttctg gtgttatcgt
    31921 aaatcgagct gacggcatag tatccccctt tttattttta tctttcaata ttttacccct
    31981 ggtactcatc aatgatacta tatataaata ctgttttaat ttcaagaaaa attatagact
    32041 tctaaaaaac atcttccgca ccccataagt aataaacaaa tatagttata ccaatagttt
    32101 tgattcaaaa atgtttcaaa aattaatttt cactatacac cctttgattt tgtaaattta
    32161 aaaaaaattg taaataacta tcgccaataa aaatattttt gttgtcagta tttaagaatt
    32221 ttattatatt cttaatatat atttcaataa cggaggctct ttaatggctt taattcagga
    32281 agttaccgat gcaacatttg actcatcagt gttgcaaaca agtggaaaag tactggttga
    32341 tttttgggct ccatggtgtg tcccctgtag attacaaacg ccaattttag aaaggcttgt
    32401 atcaaatggc atcaatgcaa aaatagttaa atgcaatacc gatgttaatc acggtactgc
    32461 ccaaaagttt ggcataagcg ctataccaac tcttatactt tttgacaatg gtaaagaaat
    32521 tgaccgtatg gttggtgtgc agcccgaaga agtcttgaag cgaagactcc aatagccccc
    32581 tactgttgta tcaatacggc tgattactca gccgtattgt ttaatgaaaa aaatttacaa
    32641 accgttaagc aacccaatgc aggtatattt ttaccaagta tttttattta acataagaaa
    32701 agatgcgcga attatgttga aagataccta cagaacatcc catgacctta cttatgacat
    32761 cttcattaag caaaatattt ttagtgccag catacataca ttgcccatta ttcaaaagta
    32821 gcaccctgtt tatccatggc attatttcat caacatgatg cgtaacatag ataatatgtt
    32881 tacctgtttt aatcagcatg gttaattgct gcaaaaattc ttcgcgagcc ttcatatcca
    32941 gaccattgca tggttcatcc aggataagaa tatccgggtc ataaacaagt gcccgcccga
    33001 tgagaacacg tttttgctcc ccatacgaaa gcttgccaaa cggagtctgt gcatagtgta
    33061 ccagttcaag tgagtgtaaa atagtcaatg ctttttgttc catggcaggc gatagctcct
    33121 ggtataagcc catgctgctg aaaaatcctg atgcaactac ttcagcaacc atggtttcct
    33181 gtgggtagtg tgtttgtaga taatcactga caataccaat gtgtttgcga acatcccata
    33241 cattgacctg ccccaatgtt tcacctgcaa tgacaacctg tccctctgag ggaaaaacca
    33301 atgtggcaat tatgttcatt aaaaaagatt tccctgaacc attaggtcca aggacagccc
    33361 attgatcacc cgtattaaca gtcagattga tatttcttaa gattatggtt gaattacgcc
    33421 acaatgatac attatgtaat cgcaaaagct ccatagtata tgaaatagca catatagcta
    33481 tggagcttca aattttttaa accatttaca atgttggtat atttttaatt ctgtcctctt
    33541 taatcttttt ttccttggct gctttttctt gttcagcttt ctctttttca accttttcct
    33601 gttcagcttt ttcctttgct aatttttctg ccaaaatttt agcttcttgt tcattttgta
    33661 atttaagctt taatgccttt gcttcttctg ccttctttaa cttttcttca tttttgcgct
    33721 gttttgcttc tttatccgtt tcaattacct tttgaatctc ctgtttaatc tcctcttgaa
    33781 ctgttgtaat aacaggaata tctttaacct cctgttttat ttctttaaca gtttctttag
    33841 gtatttcagt gggtttgatg gtttctttta ttgaatctat aattttttgc tgttcctgtt
    33901 ctgctactac ctcaacttta ttctcatcaa tcttttgaga tattgttgca gcagttttgg
    33961 tatccagagt aaccgtttgg ttttcttcta ctatcacttc tatatcttca attaccgttt
    34021 cattattaac cggtttaacc ataaccttac cagaaacaac atcaactctg gaagcttttt
    34081 catcggcaac caatctgaat gaagttcccc gcactgcagc tataacggtc tttgctttta
    34141 tggcaaagcc atcacccttt tttaacttac ctaatgtagc aaatgttttg cctttctcta
    34201 atgttaaaac aacatcatcg ctaccagctt tgataagtga gcttacaaca acagtggtat
    34261 tttcattaac acgaataata ccactatttc ggtataggag gtcaacgaca gagtttgctc
    34321 ctgttactat tgtatcacta atagtaagaa cttgcccaac agcaggaggc ttattcccct
    34381 catgtgaggt aatggtaaca tcaccaaaaa aggaattgat ggtaacaata gtatcaaccg
    34441 cctccttttt tgagcatgca gttagtatca aaaacaatac taaaattata cttttatatc
    34501 tcatgctatt cacccccatt caataaaatt tatcacatca tgcttgccat aatcaactca
    34561 ccaacacatt tcccatctac agtgcaaagg acgtgacgag attatttccg cgcagtttgc
    34621 gtacagcata atgcttctat ggtaaacttt tgcaaataac gacaccatac cacaactgct
    34681 ctatagtaaa tgcaaaaatt gctttttgta cacagtttat accagtgcta tgatacttat
    34741 aaatcactat aaaagtgcaa caaattttag actacatcag ttattatatt ttatgaatat
    34801 tgactgcttt tacacctgtc aacaacaatt aatggctata taccataaaa aaatagttat
    34861 cgttttatat cttgacattc tgaactgaat ataaaaaaat aatttatttg ctatagcaat
    34921 attgattgtt atacatatgg aatggacatg tatggaccgg attacacaaa atgataccct
    34981 catagacacg ctgggcaatt gtaccattga ttcccctctt acaataccaa aatttataag
    35041 cgactcacag cgtattgcag tggacataga ggctgacatt attgaacaat ctcttgaaaa
    35101 ggggaaaatt ccctcatttg aaaatgccgg acctcgtaaa aaaatatttt ttaaacctca
    35161 aaacaccaga gcagctattg ttacatgtgg agggctttgc ccgggtatca acaatgttat
    35221 tcagggcatt gtgcgtatgc taacattcca atatggcgtt aaaacaatat ttggagtaaa
    35281 atatggtttt gaaggtcttg tcccacgttt tgggcattca tttattgaat taacacctca
    35341 atttgttcat gacctccacg aaaagggtgg aacagtatta ggttcttcac gaggaaggca
    35401 ggatgaaaag gagattgttg atacactggt agctcatgat attaacattc tttttgtcat
    35461 cggtggggat ggttcattac gcggtgctta tgatatatat cgtgaagtaa aaaaaagaaa
    35521 cctgcctata gcaattgttg gcataccaaa aactattgac aacgatatat ggtttgttga
    35581 caaaaccttt ggattttcaa ccgccgtttc cactgcagca caggcaattt atgcagcgca
    35641 tgctgaggct ataggtgcta aaaatggaat tggtattgta cgtgttatgg gtcgtgatgc
    35701 cgggtatatt gcagcttatg ttacattggc atcaaatgag gtaaattatt gttttattcc
    35761 agaggttcac tttgatttag aaggagcgca cggtttttta cctcatcttg agaaacgact
    35821 gtatgctgct tctcatgcgg tcattgtagt tgctgaaggt gcaggccagg aatattttga
    35881 tgaaccaaaa ctgacagatg tttccgggaa tataaaatat ggtgatattg gtttacttct
    35941 taataataca attggtgcat actgcaagca aagaaatata ccagtaacta tcaaatatat
    36001 tgatacaagc tatctggtga ggagtacccc tgcctcaagc gatgatgcag tatattgtat
    36061 aatgctttca caaaatgcag tacatggagc tatggctggc aaaaccggtt ttgtagtagg
    36121 tagctggcac tcatactata catacatccc attatcgctg gtaacaaaaa agcgtaagcg
    36181 aattgatccc catggatacc tctggtcaat tgtaaaacag agcacctgcc aaccagattt
    36241 tttttagcca gtacaaataa tattttttta taaccacagc aacaaactaa aaagagcacc
    36301 agtgaatggc ataaaaaagg acatcgaaat tcacaaaaca gccatcagta gcaattttcc
    36361 gtaacacagc attatattat atgcagggtt caataaagca cattacagta tgatacataa
    36421 aaaaatgcta tgctatagta tgtgatataa aaatttttaa ttgtattttt atttatttta
    36481 ttttattttt ttagaattca tattaaatta ttgactatac tggtatttga gatatgactg
    36541 ctaatgatat ggatcaactt tttaaaataa aacagctcta tgcaaacatc ggcaagctta
    36601 taacttcttc tctggagata gaacatatac tggaaggcat catggaagaa gtccacacct
    36661 tttttggacc cgataactgg agcttgttgc gctatgatcc taattctgag gaactgtact
    36721 ttgtcatatc gcatggtatt gattttgaca gtataaaaaa tattcgtctt caaaagggtg
    36781 aaggcgttgc agggtatgtg gtagaaacaa aaaaaccggt ttttattccc aacaccagtc
    36841 atgatacccg atttagtgat aggattgacc gcattaccgg attttctact aaatctgtta
    36901 tggctgtacc tctcatattt cgtgacacca tgtatggtgt tattgaactt attaataaat
    36961 caacaggtgg gattttttct tctgatgact atctcatatt gcaaactatt gcagactttg
    37021 gcgctatagc atttgctaac tcactgttat acgaaaaagc attaacgtta acagcacttg
    37081 attcactaac aggactttac aacaataaaa aactcaacga tataattgca tcatggtcaa
    37141 aagacgaagc ccatatccga cgagatgaca ctacccacgt catagcagta ttgaatatag
    37201 atataaaagg ccttaatgaa attaatacta tagctggaca cagggatggt gatcgtgtta
    37261 taaaacatat atcacgaata cttaaaacca gcctgcgtga aaacgacatg actttcagaa
    37321 caggcggggg taattttatt tctatcatta attgtgataa ttattttgat atgaaatcgc
    37381 ttgtggaacg tatactgcac aaaatcagcg ccataactgt tcccgattat cctgatctgc
    37441 atatacaggt aagtcatgga ctgagcattg gcacgcgtaa ccgcatcaat gagcttattc
    37501 aagaaaccct tgaacaaaaa tcatagatat cactacatct ggattcatca atgtgtttgc
    37561 aatgattaat aaacattaac cctttttacg aagttgttca aaaaatgact tcagcaaatt
    37621 tttgcactct tcttccagta tcccaaaaat aatctgtggt ttgtgattaa gcacaccatt
    37681 gccacataca ttaagaactg ttccgcatgc accaaaacgt ttatccttaa caccaatata
    37741 aagtttctca atacgtgcat gaacaatggc accggcgcac atagcacatg gctctttagt
    37801 tacatacatg cgacagccgt ttaaccgttc gtttttcaat accatggcag cctgcttaat
    37861 ggcaattaat tcagcatgtg cagtggggtc agagcattgc cgcgttgtgt tatgtgcagt
    37921 ggcaatgaca ccatctttaa aaactatcgc cgccccaaca ggtatttcac cctcatcatg
    37981 tgctttttta gcttcctcaa gggcgatagt catataaaat tcaggattat tgtcattatt
    38041 gttcatggta gtttcccaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38161 attatatatt atgtatgtca ttaatacagt aataaaatat tatttttgct atactctatt
    38221 tataatatgg gaggtttgtt atgatgcgca atccactggt agatcatcga gatgtatatt
    38281 ttgttttatt tgaagtgctt gaaacagaca agctgcaaca atttccaaag tttgctgagc
    38341 atgataaaga catgtatgaa gctacaattg atttagcttt aaagatggca acagaagagg
    38401 tttatccagt aaataaagaa gcagacaaaa taggtgtacg ctatgatcct gatactaaaa
    38461 aggtaataat tccacaaggt tacaaaggtg ggttacgaaa atataatgaa gctggttttg
    38521 ttggcatgat cgaagaccct gaaattgggg ggatgggtat gccaggagct attgctatcg
    38581 ctgtaaacga gatattcacg tcagcaagtc tatcgtttac cacctatccc ggattatcac
    38641 atggtgcagc agcactcatt gaaaattttg gcactgacga acagaaaaag gtgtacttag
    38701 aaaaaatgct gagcggacag tggggcggca ccatgtgcct gaccgaacct gaggctggct
    38761 cggatgttgg aaatctaaaa acgaaagcca taccacaacc tgacggcact taccgtatac
    38821 agggacagaa gatattcatt acatcaggcg acaatgactt ttatgaaaac attatacatc
    38881 cggtattggc acgtattgaa ggtgatcctc ctggtaccaa gggcatctct atatttattg
    38941 ttcccaaata caggattaat cctgacggta gcataggtga attaaatgat gtagtatgtt
    39001 ccggtataga acacaagatg ggtatacacg gttcggcaac ctgttcgctc aattttggtg
    39061 ataatggtac atgcgctgga tatctgttag gacagccacg caaagggatg aagatcatgt
    39121 ttcacatgat gaatgaagcc cgacttggtg tagcctgtca ggggctggca ttatcgtcta
    39181 ctgcatatat gcatgcagta acatatgcac gcaaccgcat tcagggagtt catgttaccc
    39241 agatgcttaa tcccgatgca caaaaggtag ccatcattga acatcctgat gtgaaacgca
    39301 tgttgttatg gatgaaaggt tatgtggaag ggatgcgcat gctcaattac tatctggctc
    39361 ataatgcaac cttaattgaa gtattagacg gtgaggcaaa aaaggaggca caggctatag
    39421 ttgagatact cattcccatt gcaaaagcag gctgcactga ttcggcagtg cttataactt
    39481 ctgaagccat acaggtatat ggcggctatg gatacatatc tgattatcca gttgaacaat
    39541 atatgcgtga tgcaaaaatt actcagatat acgaaggtac gaatggcatc cagtcaatgg
    39601 atttaacaat gcgaaagata ctgatgaatc ctgaacagta caattatact atcatgaaga
    39661 aaagaatgtc agaagcaatt gccaatgcaa agggaattgt tgaagaaaaa tacattaacc
    39721 ttgttgaaaa cggcatgcaa aagatggatg aggtaatttt gatgcttaaa gagcagatgg
    39781 ctgcagggaa gtttttacat atctttgcag catgcacgcc agtacagcag gctatgttta
    39841 tgctaacatt agcgtggcta catctgtggg ctatgactct tactattccg aagatgaaag
    39901 cacttgttgg cgaaaagaaa ggggaagaaa gagatgcgtt tttatcagaa aacgaagaag
    39961 catcatacta ttcaggcaaa gttttatctt cacagtttta tttaggagca gaatttccta
    40021 aattttttgg aaaaattgat gcgatactat ttaatgaaac tgcgatcatt aaagccagta
    40081 aagatatctt tactggagcc ttattagagt aaacaggatc aaccatcaaa ataatatgag
    40141 agggtgttgg catcctctct ttttttgttt aaactataaa aatcatccac tcattttatc
    40201 aaaaaccgtg tttttcataa tttttattgt aaaaattgtt tacatccttt ttgaagtttt
    40261 ataaatatgt agcaatagaa ttttattttt gacatataaa cagattttta tactttttaa
    40321 tactctggag ggaaacaatg gattttgaaa ccacaacctg tatttcagta gagc
//
LOCUS       DAOSOX010000002        39226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1072_length_39226_cov_9.509816, whole genome shotgun sequence.
ACCESSION   DAOSOX010000002 DAOSOX010000000
VERSION     DAOSOX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 39226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39226
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1072_length_39226_cov_9.509816"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..687)
                     /locus_tag="PLX22_00220"
     CDS             complement(28..687)
                     /locus_tag="PLX22_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT18359.1"
                     /translation="MSRLTRKDILQAGIASLQTAFFTGTIAPLYHCFAAISYHEAMHW
                     KKVDTKTVQCMLCPNQCMLRNGDKGACKARQCINGTLYSLVYGNIAAYHIDPIEKKPL
                     YHFMPGNEALSVATAGCNFSCKFCQNWQLSQSYPHELDSKHITPVQLVNNALNKKTPI
                     IAFTYNEPTIQYEYIYETAVQAKKKHVACVIISNGYCNKNASVLLYPMLDAIKILRFN
                     N"
     gene            complement(700..2214)
                     /gene="amrB"
                     /locus_tag="PLX22_00225"
     CDS             complement(700..2214)
                     /gene="amrB"
                     /locus_tag="PLX22_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT18360.1"
                     /translation="MLKKIITILLIPFLLQCTSKSYSITKQSKLAGTWYPDNAQQLSQ
                     LISSLTRNAKHHAINHTPMIIVPHAGYTYSAYVASKAYGAIVTYKPDIIIIIGPSHYE
                     QFTGFALPVYDAIATPLGTIPVDKKTVQKLSANNNFVFNNKAFEPEHSVEIQLPFIQY
                     LFPGIPVIPILTGSTTPDTAKEAGCHLLYAIAHYKKPLFIISSDFTHHGPRFFFTPCS
                     NSNKSKQMQCIKQGDQQAIDYILSCDADKLYDYCKKTGITICGVNALIFGLSIYEKVT
                     TKQLAGYATSADITGDFDNTVSYAAIVIDGMLNTQAKPYANNQPSMLSDNDKAFLLSL
                     ARQSIQSYVLSKKPFHYSGTIPEACLHNAGAFVTITIQGNLRGCIGYIEPIKPLYKTV
                     IECAASAAISDPRFEPLSPEEYSTIRIEISVLSPLKKISSLDELTPGKDGLVVVKGNR
                     RGVLLPQVAQEFNFTKEEFFQQTCNKAGVYPFEYDSIIMYTFTATVFHERTPSP"
     gene            2706..3434
                     /locus_tag="PLX22_00230"
     CDS             2706..3434
                     /locus_tag="PLX22_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18361.1"
                     /translation="MKRFAFLILLIGFMFVASAGFSTTYVLHLNGMCSTHWLDGKSKG
                     GESRLANAPGYVSVNCYVDNNNTIAYSASQFKTNYLDVYCKGSNWCYVVNYSAGDAIV
                     GYINANYTPQWNIAYVYTTAGAGGGSEISIRGISDLFTCNLGGQLGVSTIRNMYNHND
                     TNGIIIYHIGGYKGIFGASALLPGEDDGAVAYHSSAACVNSGSYNNLCNCTHWTNHVI
                     AYTCSGYYLNHYEMKMKFITQLGW"
     gene            3745..4848
                     /locus_tag="PLX22_00235"
     CDS             3745..4848
                     /locus_tag="PLX22_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18362.1"
                     /translation="MKVKLTHISLIIIVIIAGIMLYITHYANKNDTIAEEAAGESSHG
                     YSTVLEEMGLISPQQALQDYKQLSIYPPNSRPLSKENIDLLMPNRRYEQPLPIEEGSD
                     IYYLFTADKYRVVGDDTITFVLIVLRGTKPDSERIPIAITKSVIKKGLNKNKAKYYAE
                     LSLAKNTTGDYTAELTCSQSFSSEKKSSAYWAEVEFNAGNEHVEAAIPFEYFPENTIP
                     AKFTGNFKERIADGSLYIDVEIDVLRKGYYIVDANLFDAEGEPVAYTVQKQELQSGKQ
                     MVPLLFFGKVLLDKKAKPPLTLKDLRGYRFIEATTPDPEFMDREMMKPYDGEYKTRLH
                     SLQGLSDKEFEDAMKRKRIEFLEKENTGDIGVQ"
     gene            4838..5251
                     /locus_tag="PLX22_00240"
     CDS             4838..5251
                     /locus_tag="PLX22_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT18363.1"
                     /translation="MSNSRITISSLACCISLIFFWAIITLHGCTVSHNTNNSITIVIV
                     SAKNCESCSMTDDTIQYVQTHYPDSIIKKVDINNPDGAMYVSTYHLWRVPVYLFLDNK
                     GSELYRLEGEQKRSDLDTAINIVSKQYEHVNILKK"
     gene            5509..7440
                     /locus_tag="PLX22_00245"
     CDS             5509..7440
                     /locus_tag="PLX22_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT18364.1"
                     /translation="MKRLFIFIVAIVLSGTASVQADDEAVQLEDVIVSDTLLYPGAYQ
                     SIKGNTVKDSSNNDLISVIKNQIPSFYTGANRVLGYGIARSGAATMSMRGIGKSGWGP
                     TTGIPILYNGMDTTMGIMGHPVADIMNMKNVDSIEVLIGPQPVLFGSGAMGGVINVIT
                     KRQKEDGYTTEIQGSYGTWNTTEDYLYHMGKFGKLDYSAGYQFQKTDGDWEQTINGKT
                     FTSEFMQQNGTVHVGYALSHNWYAGVDSYGMKQNIHDPGPEGIDALSEIPLTNQLNLL
                     EEFDITRGGVVVKLAHDYGNYSGTLQAEGNFGNHESKQSNTDIDKFESDDSSYAVRLK
                     EIVKLWQGNTVTTGAEWRRYGGTAKDIENDSYYMKDKFMNDTSIYVLMDQNFLKNILS
                     VNGGTRYSYNSEYGDYIAWQGGAAVRPFKKTKFYSSVARGFKFPDIRQVYLKGMYPAI
                     NPNPDLKPETYTMAEIGMEQKLGDTITIHMGGYRIWSDNMFVYTSQWENADEFSYNGA
                     EAMITVQALKWLSFNAGYSYIDNKQENGYLPYVPAHKANGRITLTIADFTIQLDGEYV
                     NELYADTQGLKEFDSYFIINSKMSYTFLQKYTAFISLYNITDKEYSTFAVLVPPLGDS
                     YCEYPMPDFHFLAGLQATF"
     gene            7677..8495
                     /gene="amrB"
                     /locus_tag="PLX22_00250"
     CDS             7677..8495
                     /gene="amrB"
                     /locus_tag="PLX22_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT18365.1"
                     /translation="MWIRQPGFAGSFYPANPDKLKNDIQRYIANAKIVSLDKPVAFIS
                     PHAGYIYSGPVAAYSYAQLKHKPVDVVIILAPSHRVRFNGASVISSGIYQTPLGEVQI
                     DETIAQKLLQNNVFEEIKEVHQYEHSLEVQLPFLQVVLPQFVLVPIIIGTIDFTTCKE
                     IGSEVAKILHDEQRRFVIILSTDLSHYYHYDIAKKIDSHFIESLRKFDELYLYEALRS
                     DKAQACGEGPVLAGMIASKQLGATKVEILHYANSGDTAGPKNEVVGYLSAAFVQ"
     gene            8575..9156
                     /gene="amrA"
                     /locus_tag="PLX22_00255"
     CDS             8575..9156
                     /gene="amrA"
                     /locus_tag="PLX22_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOT18366.1"
                     /translation="MIVLNDEQKIKLLTLARKTIEEYLNKRTLPKVDFNNEFNDEVFK
                     QKCGAFVTLHKNGALRGCIGYVKGIKTIPEAVVDMAKASAFEDPRFEPLTKSELDKID
                     IEISILTPIEPVNDISEIVIGRDGLIISSGFRSGLLLPQVATEYGWDVKTFLEHTCYK
                     AGLPPDAWKWNNTKIEKFSAIVFSEKELKLRYC"
     gene            9205..9651
                     /locus_tag="PLX22_00260"
     CDS             9205..9651
                     /locus_tag="PLX22_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18367.1"
                     /translation="MITKKMFGVSLCMLVSFMLFSCSNQRDYSDIENLLNRWANSVRY
                     LNYAEYTKLEAYPKDSNTFKTMYQNYYITNLQIVEVDDKLHNGTDSDGKPYEFKNVKF
                     IATIINRQSGKGEQVVNGDIKLIRYATGTKKEKGWLLSNRTLIHQQ"
     gene            9658..>10349
                     /locus_tag="PLX22_00265"
     CDS             9658..>10349
                     /locus_tag="PLX22_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18368.1"
                     /translation="MINIFNIQVKKEKIIIAFIVIVISFLTATSIKPQVQNKQLIDNY
                     NKQAIEYYNKKDLTKAIIEWLKILEIDPNNEDAQRNIELVYEEKHKKDISFQKAKLYY
                     RLSRKVLPEDIDAAKNNSSIAIEHFIVAYRLDPNDPELQILKDELKLLSDEIKLELAK
                     KRLSEEIKKKYYALLSEAQILMNEKRYEDAIKVWDEMLRLVPLDAVAVEGKRQAELAI
                     NNRLKFEKIMAL"
     assembly_gap    10350..10449
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10450..11187
                     /locus_tag="PLX22_00270"
     CDS             <10450..11187
                     /locus_tag="PLX22_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18369.1"
                     /translation="NENNPYYSIVQPLIESGKRLYNQGNIAESREQWVKILNLFPQNR
                     IATEYLLRCDLAKNPESFQKFAEKIVNDGKKLLAEKKYNEAFKKFDLIATIDPQYPEI
                     QNLITQSKVEKRKTGGEVADIGFRQRYNMALQLYQQGGKNNLEKALAEFKIINNKYPD
                     NIQVAIIINKIESQLRFQVAEQVQGKKLTPRQQELIREYYFKGINYYTNNQFDLAITE
                     WRKVLAIDPDHEKAKNNIKKCLILLGR"
     gene            11191..12795
                     /locus_tag="PLX22_00275"
     CDS             11191..12795
                     /locus_tag="PLX22_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18370.1"
                     /translation="MSKEKASDNEKNKKTSKNKKKNIKDDNSNTIATSKKEVRFSLRL
                     KFSLAIILLVSSIITVMTYYFIQQESELLRKQILQFANREVERLAAIARESISQKDEL
                     PLVGAVQDLKNLEFIDYVYVIDTNKRIIQNFDPQMNGTIIDDDLTSKASNNTDSGKPL
                     VVVYRDNKKNVNFYDLSLPLFHTVQTVRIGTVRLGFSEEIILNEIRKLTITILLIGSF
                     FIVISIVGSIVLASVIIKPIKKLTEGAVIIGGGNLDHKIEIESSDELGKLANEFNQMT
                     QRLKVAQQVEIESRIMQEQIDLAREIQEGLNPMGFYNKSGIEIKGFTRAAKGVGGDYF
                     DYIDIDEYRVGALISDVSGKGVPASLVMVMIRTVFVSYIRRGDVQCADVVRAINDSLS
                     ADFAIDKFATLFFMIYNRSNNELSFSNAGHGPLFCYRSSLDACTLTKLEGVPIGIMED
                     VDYKQAKVLLNIGDIIIMYTDGVTEMRNADKEEYGQKRLKDLVVANKQLNAKELSDLI
                     VKDVDNFRGDVPQHDDMTLLVLKRTQ"
     gene            complement(12820..13143)
                     /locus_tag="PLX22_00280"
     CDS             complement(12820..13143)
                     /locus_tag="PLX22_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT18371.1"
                     /translation="MEIELKDIGEHKVISVSGEVDLYNVSELKKALFSVTDGTYSSVV
                     VDLKDVNYMDSSGIGALVAGQKKMRAHNGKFALINIHDDVLNILKLATLDRFFSIYED
                     ENDLI"
     gene            complement(13225..14295)
                     /locus_tag="PLX22_00285"
     CDS             complement(13225..14295)
                     /locus_tag="PLX22_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18372.1"
                     /translation="MENKVHLLIIDDEDIILKSCQRILRKREDLTIDTATNGREGLEK
                     AKAKKYDIVITDLKMPVMDGMEVLKTLRKEQPDVTVIIFTGFATVDTTREALKLGAFD
                     YIPKPFTPDELSNVVDNAIKARQNKAESAMLDLLAIVSHELKSPVSVVHTTAETLYKG
                     YFGNLSPDQQKIIETILRNCQYLEDIIRNYLDLSKMELDSLESFSQQIDLYEEVIKPI
                     VEMPENTNNIKNMKLELNIQVKPKIMGDANLLKIVVTNLINNAIKYGQANTTINIELT
                     QQNNEYVFSVYNEGVGIPEEDMEKLFKKFSRLKQKGTEGIKGSGLGLYICKSIIEKHK
                     GKIWVESEYGKWVKFSFSLPLS"
     gene            complement(14415..15791)
                     /locus_tag="PLX22_00290"
     CDS             complement(14415..15791)
                     /locus_tag="PLX22_00290"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT18373.1"
                     /translation="MFHNNNIIKNIIIEEDKAKGVFFEFAEVLKKAGVDLSIVEIMDM
                     MNAISHISILEKEVFKQTLATTLIKDYTDIPVFNQCFEQFFERKKGNDSLLDIYTLHA
                     HSEDFIFTPEETSLLNSLIDNFLESHPQSFFKKSNEYLTKIFIDEELSSESGGAVGLS
                     LFNKRSQFASAGANDSDYQELSPQLFEVIMGMIRKRMFERTMGSTIKQREDYLLNKYI
                     YQLKPDEIKEMRELIKRFGQKMKNRISLRKKRVKRGNFDIKKTLRRNLQYGGVPFKIF
                     HRDRKIDRPQLVVLCDISSSVNQYSRFMLLLTYTLQSLFAKVRSFAFISNLVEITDLF
                     REMDPERALNSIFSDTNFTYGWGSNYGNSFNQFIQNYSDALTSKTTVLVLGDARNNNQ
                     DPGLDSFIKMKERSRNIYWLNPDKQHLWNWSDSIANVYIPYCSEMREVQNFLDLSHFI
                     DTLFIQKR"
     gene            complement(15798..16706)
                     /locus_tag="PLX22_00295"
     CDS             complement(15798..16706)
                     /locus_tag="PLX22_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT18374.1"
                     /translation="MFTSVENTIHELEKQKYICSKDIATTVYLALKMEKPVLVEGPAG
                     VGKTDLGKCIAKSLGFELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKDKLSRILED
                     QATLKDAVNKLTSEEDVFFSEKFLVARPILKAITNDITSLLLIDEIDKSDSEFEAFLL
                     EVLSDYQVTIPEIGTIVAKNKPFVLLTSNNAREMSDALKRRCLHLYIDYPTKELEERI
                     IQLKVPECGNYLIKEIINAIHEIRKFPLKKDPSISETLDWAKALTLMGCEVLNRDIAI
                     DTLNFILKYEKDIELVKKNLHSIIVH"
     gene            complement(16828..17763)
                     /locus_tag="PLX22_00300"
     CDS             complement(16828..17763)
                     /locus_tag="PLX22_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18375.1"
                     /translation="MGDSSLNMVESTYVDRVRNHSLFKESWIAFASDYPPSHCLLDFN
                     QLPSTDVALHGGDLGFALLWIVQSVLMRCVYFDINQLQPWEIPALTPVTIGAMAHTEP
                     DTARMTARYIDNNTLSLSGHKKYITGGKDADLLFVTARRTGEDKFTKIVYIQTYQLPE
                     NALQQLDMSILPTINHATLTLDDFPCNALQCSPVKDKELRKILKKWGMVERALIGEAY
                     IGYLCYLAKNITMPDQNELIDEVENLLALQKTFTRQQLENAFSNNYIDTLGDVARVLS
                     VTQKISNYCMENPQTTGDSITLRLHDLNLFNKIRL"
     gene            complement(17765..>18058)
                     /locus_tag="PLX22_00305"
     CDS             complement(17765..>18058)
                     /locus_tag="PLX22_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT18376.1"
                     /translation="AIEYAELVYNGLWFTKRREALDAFINESQKNVTGTVKVKLFKGS
                     CMAVGRKSPVTLYEPDIATFDDSALYDHRDATGFINLFGLQTKIEALLNLKRK"
     assembly_gap    18059..18158
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18159..18550)
                     /locus_tag="PLX22_00310"
     CDS             complement(<18159..18550)
                     /locus_tag="PLX22_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT18377.1"
                     /translation="MSIKKVVLAYSGGLDTSVIVKWLLETYKCEVICFAADVGQEEEL
                     NGLEQKAINTGASKCYIEDLKEEFVRDFIFPCIKANAIYENRYLLGTSIARPVIAKKQ
                     VQIALQEGADAVCHGATGKGNDQVRFELT"
     gene            18850..19173
                     /locus_tag="PLX22_00315"
     CDS             18850..19173
                     /locus_tag="PLX22_00315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT18378.1"
                     /translation="MAQKVVSQLRTLDIYARYGGEEFIICLPETMPDDAVIVAERIKN
                     IVEECTFNDNDHLITVTISSGIASLADIDEELEMIIKHADEALYQAKQSGRNRVAVYR
                     KNGYN"
     gene            complement(19182..20129)
                     /gene="argF"
                     /locus_tag="PLX22_00320"
     CDS             complement(19182..20129)
                     /gene="argF"
                     /locus_tag="PLX22_00320"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT18379.1"
                     /translation="MIGKIPKVKTKDLLSITDFSKEEIIAIFEYTQKMKDELNHGKTH
                     NFLEGKTLGMIFEKTSTRTRVSFEVAMFQLGGYALFLSRDDIQLGRGETVGDTARVLS
                     RYLDGIMIRCYAHATALELAENATIPVINGLTDMFHPCQALADFYTIYEHNKNFKNEK
                     LAFVGDGNNVAQSLMLTAAMLGVNFALACPKGYYPAKNVVEMAFSIASKSGSVITLTS
                     DIALAVEQATYLYTDVWVSMGQEKEASRRRKNFAKYKITMKILDKCAPNCKVMHCLPA
                     HRGEEITDEVIDSPQSIVLDQAENRLHVQKSILCALMAK"
     gene            complement(20126..21346)
                     /locus_tag="PLX22_00325"
     CDS             complement(20126..21346)
                     /locus_tag="PLX22_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT18380.1"
                     /translation="MAVSKKLQTLIEQDNQYLFQNYGTRLPACFIKGKGSELFDQDGK
                     RYIDFFAGIAVNSLGYNHPALNSALHKQIDAIMHSSNWFFNKQQIEAAQLLSETSFPG
                     KTLFTNSGTEANEAAIKLARKYGISQSNDKYHIISFTNSFHGRTFGAMSATAQEKIRS
                     GFGPIVPGFIYVPFNDVTQFKKTLEANKGKICAVITELVQGEGGITVADSYFIKEISS
                     LCKENNILLIIDEVQTGIARTGKMFAYQHYDIQPDIISLAKGLGGGVPIGAIHAQNIL
                     VEFFPKGTHGTTFGGNHLAAAAAKTVLEHCKKESLLNNVNTMGKLIVEKLSLMKKKYP
                     TLIIDVRGIGLHIGIELSMAGQPIVVEALKKGLIINCTAEKVIRIMPPLTINKKLVTE
                     GLSILDSIFAGVTA"
     gene            21564..22109
                     /locus_tag="PLX22_00330"
     CDS             21564..22109
                     /locus_tag="PLX22_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOT18381.1"
                     /translation="MICERCKLREATVHLTEIIRNEKTEVHLCDNCARDMGLSSHGGG
                     FSLTVSDMMSFLDVKDMNDMNDMVDSNTCATCGWTLIDYKRTGKLGCADCYSSFKEAL
                     TPILSGYHGSTFHIGKMPLKYIEHRKSKVMLDSMRLKNVATLSLHDLENMLEVAVENE
                     NYEEAAKLRDEIIKLKNQQGA"
     gene            22109..23158
                     /locus_tag="PLX22_00335"
     CDS             22109..23158
                     /locus_tag="PLX22_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18382.1"
                     /translation="MFEQLLEQSPFWARIGPLADVAMTTRVRFARNLPSLPFATKMRE
                     SDITILETIVRQAVIASTLFQNTQFVNLRDCSSDDRRFLCERDIITHEMELSDYSSVV
                     FDHQQNFSILINEEDHIRIQVIKPGLQMLEAFKLADEIDDELNKYISYAFNDDIGFLT
                     TCPSNVGTGLRVSAMLHLPMLTATKNMGEVIKIVREYQAQIKGIVHDATKTVGSIYIV
                     SNKATLGKSEIDIIEEVDKVISIIIDLENNAREEYYQHHKDMLEDMIWRSYAIVAFSR
                     TMNYAEAIEHLSLIRLGVVLSVLKNIELSAINDLMIHIQVSHLQKIAGKIFATSEEGD
                     SFRAAYLRKRFGDKE"
     gene            23165..25630
                     /locus_tag="PLX22_00340"
     CDS             23165..25630
                     /locus_tag="PLX22_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT18383.1"
                     /translation="MFDYTKRSRKVLEVLAQAEGRRLNSDALGPEHIMLALLKDDDSV
                     AARILKNMGVNFEILRRNIEQSIRRSGSTIILGNLPLSARFTRIIEIAKDEARKLKNN
                     YVGTEHLLLSIFRDGTCSGIDTLIRAGIDYNVLKSEVQKILGVTPDGSTQQKGNDKTK
                     TPTLDEFAIELTRLAAEDKLDPVIGRDTEIERVIRILSRKTKNNPVLIGEAGVGKTAI
                     VEGLSQRIIHHDVPEPLFNKRVLSLDLASVVAGTKYRGEFEERLKRIMKEIKGSDNVI
                     LFIDELHTIIGAGAAEGAIDAANILKPALARGELQCIGATTLNEYRIYIEKDAALERR
                     FQTVMVDEPTADEAVEILFGLKERYEIHHKVRYTDEAIRKAVLYANRYIHDRYLPDKA
                     IDVIDEAGSKARLENCERPVDIELLEQEIEELNDRKNNLVKAQEYEQAASIRDTIIER
                     KALLSQKISDWQQRINDYAVVVDEKQIATVVAQWTKIPVEKLEESESQKLLQMEQELH
                     KRIIGQDNAINVISRAIRRSRTGLRSAQRPIGSFIFLGPTGVGKTELAKALAEFLFDD
                     DDALIRFDMSEFMEKHSVSRLIGAPPGYVGYEEGGQLTEKVKRRPYSVILFDEIEKAH
                     PDVFNILLQVLEEGELTDNFGSVTSFVDTVIIMTSNAGNREFQKIGKMGFNEINANQI
                     QFQNVDEEVKRLFSPEFLNRIDAVVHFHKLNEHHIRQILDLLLQEVNEHLTEQGIELL
                     VSHKVKNYLVSKGFNDKYGARYLRRAVQNEIEDRLAVEILNGKFKGCHKVYAGIKNQT
                     IYFKPEKNGTVGDINTPEVVHYK"
     gene            25648..26904
                     /gene="cls"
                     /locus_tag="PLX22_00345"
     CDS             25648..26904
                     /gene="cls"
                     /locus_tag="PLX22_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOT18384.1"
                     /translation="MKKSQKKTHGRLNHRFKGSGHRISYSDVIHKIKNRFFLPKFLKS
                     IKKYGISGFSGGNKVTLINNGDLFFDELFAAIDSATLSINLETYIFNSDDIGWKVARK
                     LAAKAKKGVDVNIIYDAVGCINTSGELFEYLRKNKVELIEFHPIIPWRKYFNLYLRDH
                     RKLMVVDGSTAFVGGMNIGNEYAGEKYGGENWRDTHLKIEGPAVRDLEYFFLENWHRQ
                     GGALVDIARYFPVINHKGDTIVLVLSSYSRKNVKPIYQTYLAAIANAQKYIYIANAYF
                     VPDSKISRALIHAAKRGVDVRILLPSVSDIPFVQYASRYLYKRFLKNHIRIYEYIPTV
                     LHAKTAVIDDIWSTVGSTNLDRRSFNANLEVNAIVLDQNFGNKMKKQFLKDIKQSKEI
                     VFEQYQRRSSTQLIKEWFCYRFRYLL"
     gene            complement(26933..27748)
                     /locus_tag="PLX22_00350"
     CDS             complement(26933..27748)
                     /locus_tag="PLX22_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT18385.1"
                     /translation="MDLSELLPKEHLISIKSTEKQLVIKELIHKLQELGKLDNADRYY
                     TQVIHRETLENTGVGNGFAIPHVRTDSVKKLLTVFGICNEPIEYESFDKQPVKYVMLS
                     IFPTSLSTKYLYLVGMMARIFSSSEKRSKIDEARTPSKIYPILTKEAKLYFESLTEIN
                     KEDEHIENLSGIPSTDLDLLIRLDQLYRLYDTGDTSEALTRKINELRKFIDNRSLSYY
                     ERMRKKCQNPFAILEKNACGGCHMVIPPVEMAKIKSKKHLAVCTYCGRFLITV"
     gene            complement(28006..29292)
                     /locus_tag="PLX22_00355"
     CDS             complement(28006..29292)
                     /locus_tag="PLX22_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT18386.1"
                     /translation="MLPPFLQSLVNNESVHSARYVHYSPDAIVTLLSLLHNPHTAIKT
                     IHIAGTNGKGSTAYMIAAMLQAAGYRVGLYTSPHLLRYNERITVNGNEIADETIAHFS
                     TIIEQLCTKHNLHITFFDAFTAMAFLYFQNSVDIAVIETGLGGRLDSTNVVTPLVSVI
                     TPIAYDHTAILGNTLQQIAYQKAGIIKQNTPVVIAHQHPEALATLRHEAALNNAPIIL
                     FNTNIHCSITSNHPVLTCDVVCNHPLFGGLQKCSNVTIPLAGSFQAYNAAVAIAAVLL
                     LKNYYWAIVTDDIIKALSTITIPGRSQLFGEDPLILFDCAHNPEAMQTTVEALINTYS
                     GYDCIFCVSFMNDKDIHSLLTIIQRYTQHPVYYLPLNDSRSYYPDDEVIKNYHLIITD
                     DIHLPVTIAKKSPGKTMVLFTGTFRLYPLVQTIHNA"
     gene            complement(29295..30134)
                     /gene="aroE"
                     /locus_tag="PLX22_00360"
     CDS             complement(29295..30134)
                     /gene="aroE"
                     /locus_tag="PLX22_00360"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT18387.1"
                     /translation="MKPDANTTVYCIIGNPVRHSLSPVMHNVAFEHLHINAIYVAFQP
                     QSAQKAIEAMKTLGIQGASVTIPFKVELASLCDEVDDLALEIGSVNTLINVNGNIKGY
                     NTDGNGLIKALEHHAVDYVHKNVLIIGNGGSARAIAYTLAHNNAHIIICGRNENNVMH
                     LVHDLQHHGISVNSCLLHDLHPDTTLPFDIIINTTPVGMSPHADTSPINEHCIHPHHI
                     IVDIIYSPRETMLIKKARNKGCKTITGEYMLLYQACMQFELWTGKPAPFEIMYNALTQ
                     ALQ"
     gene            complement(30131..30478)
                     /locus_tag="PLX22_00365"
     CDS             complement(30131..30478)
                     /locus_tag="PLX22_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18388.1"
                     /translation="MNYNEIFKLTADTRWNVVAQDENLWRVGIYHPEYNSAHEIDRLE
                     KHSCPELFICVNGQCGLLLREGNWEHQLIMNPLEAIMVTSYHNGFKVDKDGFFIVAER
                     TDFTTNFIERDKP"
     gene            30865..31647
                     /gene="thiD"
                     /locus_tag="PLX22_00370"
                     /pseudo
     CDS             30865..31647
                     /gene="thiD"
                     /locus_tag="PLX22_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     gene            31687..32160
                     /locus_tag="PLX22_00375"
     CDS             31687..32160
                     /locus_tag="PLX22_00375"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HOT18389.1"
                     /translation="MHEVEQAFSVIQAAQCGDILVSGSSNIGYALPYPINQTDFALFP
                     GGIITHQDSVFKIKDAACNVEHPLADMMQKIKGNYRTAISIKNSNAIRAIAHAMDVPV
                     IQVTAPDELMNTGFDKSQIMIVVDDDPLLYIAGQNPLDVAQIVVSIKRRLHEYTS"
     gene            32144..32962
                     /locus_tag="PLX22_00380"
     CDS             32144..32962
                     /locus_tag="PLX22_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT18390.1"
                     /translation="MNIPHKQLFDFLDNEFPLMYQEEYDNSGRQIAFDESNVTGILIA
                     MDISPQIIDEAIEKKCNCIITHHPMFFRSLKNITISSVHGFMTIRLIQNTISVYAVHT
                     NLDALCWDILAKTVRLNDIKMLFPDKRFNGIGFGAIGCMQDEMLLSQFIEQLQHELKT
                     MAIIYYGNLDKKIKTIGAFNGSGSKKINAIIQSYPVDCIVTGDVGYHDAVYANNHDVA
                     VIDIGHYSSEKPLLHFLHSKLYNYLTNAMHCSDVRLIISEKEQNPMHCGVQNGK"
     gene            32952..33650
                     /locus_tag="PLX22_00385"
     CDS             32952..33650
                     /locus_tag="PLX22_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18391.1"
                     /translation="MGNEIYVMLELQAIWNKVLDSKTEIARSRHAIDFWRDEMKSIGA
                     RMSDLRSRVDQLKLKIKEKEMELHDVESRIELLDSRKNSLKSQREFESMQTELDTLAK
                     KKDTIEEQLLQYMEDVEQIEGELEIFHVEMPKRLQQIENDVQQLQGKLEVMQKQAEDN
                     AKEFNKRISELPPDLRMRFAKVIDTKGNAIVPLEDGICMGCHFQVPVYVKDEVIKSRP
                     ATCTNCGRFLYLKN"
     gene            33923..34597
                     /locus_tag="PLX22_00390"
     CDS             33923..34597
                     /locus_tag="PLX22_00390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOT18392.1"
                     /translation="MLLSIDRVLQLLAEGKSIEKIAELANVDSSDVLAIIEEARKIVN
                     QFDKQKSKSKIIIKKKNNINTQIPMEMKETDEELFDGFEVLSTIPTESVLTMYVDGAS
                     KGNPGPAGIGIVIYNNDDQFVGKVSSYIGKKTNNQAEYIALIRAMLIAIYFKTKTLKI
                     RSDSELVVRQLLGEYQVNNSQIKKLYDKVMELKKQIPNFKIEHVMRNFNERADFLAKK
                     AADKKI"
     gene            34651..36642
                     /gene="recJ"
                     /locus_tag="PLX22_00395"
     CDS             34651..36642
                     /gene="recJ"
                     /locus_tag="PLX22_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT18393.1"
                     /translation="MYTKTAELSKKTLLHLFNITDVTADLLLLRGIDTPYKAYIFFNP
                     ELINCYSPFLFNRINEAIVLIKKCIQQKIKFGIFSDSDIDGLTSLTIVSHLFDLLGAD
                     YCYRYPVGEELYGLTIPIIDEFRKRDVQCIITLDSGTRDVEEIAYAKAHGMDVIVCDH
                     HEPSDELPDAVIINPKLHTCSYPFKELAGVGVAFKLCIALLLSYTRYYEQYVIICAVD
                     SNAYHYKVMHRGCTKECGVVDDISDIQHIINKYTNTILLHYDVAAVNALQHAKLFNLQ
                     DYSGELSNKKNLLKIEPEKVFPSALDFAVTVAQKMQFQQSKKMMKFIKDVVPLVTMGT
                     IADMMPLVDENRTLVTAGISFFNKTYHTGINVIREHIAKPINADVIGWDISPLLNTPG
                     RFGKTELAAKFFLMDKSSEVKGILDEIIQLNAERKNIIQTLVNKFSESYSANANKKII
                     MIQSNEIPEGLTGIIANRIADQTLLPVIVIANKTDSTVVKGSGRVGNHLDFFSIVSQF
                     KHIFEKLGGHEQAFGFSIHKKNIEVLEKLLHDYLQQYDTCNNNIHYDYELPIDYISFD
                     FLDELKLFEPYGAGHKPFTFISRNCSIASYYIINAKHIKCIVDGTIKLEAMAWNGKDK
                     YEAIVAQKKPVDIIYTVKTNEFNGLISPLLIIIDMHLSN"
     gene            complement(36639..38609)
                     /locus_tag="PLX22_00400"
     CDS             complement(36639..38609)
                     /locus_tag="PLX22_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT18394.1"
                     /translation="MNRIIIYTCFIVTIATIAFAGDWPIYKGNLFFTGNNDEIIVPGN
                     QIKWLFLAKDKVFNPVVSDGRIYFVDKNAFLYCIDEEAGTILWLNDVHAISAQFKAYS
                     KSAGKIKYPLVYGNTVLVSDPIAIYAFDKTNGKVIWARTGLRVDETKPQGLTGIATQV
                     MVDGIYADPIIDADTIYYGTRKVFASRDIANGHIVWENKDIATYSGFPVYYDQYIITQ
                     SMNYETKAFTVFCLEQRTGKPVWQRTFSVPIKILPPVVNKNVYVPVNDTIYCLNIKNG
                     EIMWQKSYNRTITSPLSFTDRTILFSFDNNSIKAINPENGEVVSTIFVGQQSSPYFVT
                     IRDVIYIAFNEVTDKNPVAKVKAMYISTSQLWWEFVAPFPGGVSQPVAADGILFLPAG
                     NYLYAIGAGYMGSKGFDSAKTATDSDNKKNEQKSMPAPRYSEKELSQKTITPETQPKK
                     TSEQTQKQLPMRTLPVEITNDQLSPLKAKATIHYKDEDGEYKQSTAVNAKGDITVPDK
                     DNVEVIFESDGHVPKKVIVNKSDKQLNVVLDQIQPGKSVVIDNIHFDFDKWYIRKDSV
                     TILDKLVEMMKKNPGLIVEVQGHTDSTGDKTYNQKLSEKRADAVAEYIIKHGISPERI
                     SSRGYGETKPVASNETKEGRQKNRRTEFYFKS"
     gene            38983..>39226
                     /locus_tag="PLX22_00405"
     CDS             38983..>39226
                     /locus_tag="PLX22_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT18395.1"
                     /translation="MATSTQHDKSFFNTLMVATGIFLSRIAGLIRDRVFAHFLGNSDA
                     ADAFRAAFRIPNFLQNLFGEGVLSASFIPVYAKLIAQ"
BASE COUNT        12614 a         6464 c         7492 g        12456 t
ORIGIN      
        1 attatttttt acagaaaagg gttgcactta attgttgaat ctaagaatct ttatagcatc
       61 aagcattgga tacaatagta cactggcatt tttgttgcaa tatccattag aaataataac
      121 acaggctaca tgcttctttt ttgcctgcac tgctgtttca tagatgtatt catattgaat
      181 agttggctca ttgtaggtaa atgcaataat gggcgttttt ttgtttaagg cgttgtttac
      241 cagttgaact ggcgttatgt gtttgctgtc aagctcatga ggatagcttt gtgatagctg
      301 ccagttttga caaaatttgc agctaaaatt gcacccggcg gttgctactg aaagcgcttc
      361 attacccggc ataaaatgat acagaggttt tttttctatg gggtcaatat ggtaagcggc
      421 aatgttccca tacacaaggc tgtacaatgt gccattaata cattgacgtg ccttgcatgc
      481 acctttgtca ccgttacgca acatacattg attggggcac aacatgcact gtacagtttt
      541 tgtatcaacc tttttccagt gcattgcttc gtgatagcta atggcggcaa aacaatgata
      601 taatggggcg atagttcctg taaaaaaagc tgtttgcagt gaagcaatac ctgcctgtaa
      661 aatatctttc cgtgttaatc tgctcatatc aatccttcat tatggggaag gtgtacgttc
      721 atgaaagacg gtagcggtaa atgtatacat gataatcgaa tcatattcaa atggatatac
      781 tcctgccttg ttacatgttt gctgaaaaaa ttcctccttt gtaaagttaa attcttgtgc
      841 aacctgcggc aatagtacgc cacgcctgtt ccctttcaca actacaagcc catcttttcc
      901 cggtgttaat tcatcaagcg atgatatttt tttaagcggc gataataccg atatctcaat
      961 acgtattgtg ctatattctt caggtgacaa tggctcaaaa cgggggtcac tgatagctgc
     1021 actggcggca cattcaatca ccgttttata cagaggcttt attggttcaa tgtaaccaat
     1081 gcacccgcgc agatttccct gtatggtgat tgttacaaat gcaccggcat tgtgcaagca
     1141 tgcttctggt atggtgccac tgtagtgaaa aggcttcttg ctcaatacat atgattgtat
     1201 ggattgtcgg gctaatgata gcaaaaaagc tttatcattg tctgaaagca ttgatggttg
     1261 gttgtttgca tatggtttag cctgtgtatt gagcataccg tcaataacaa ttgcagcata
     1321 gcttacggta ttgtcaaaat ccccggtgat atcggcagat gttgcataac ctgctaattg
     1381 ttttgtggtt accttttcat atatggataa tccaaatata agagcattta ccccgcatat
     1441 cgttatgccg gtttttttgc agtaatcata caatttatca gcatcgcatg acaaaatata
     1501 atcaattgcc tgttgatcac cttgttttat gcattgcatc tgtttgcttt tattactatt
     1561 gctacacgga gtaaaaaaga aacgcggacc atgatgagta aagtcgctgc tgataatgaa
     1621 caagggcttt ttataatgag ctatcgcata tagtaagtgg catccagctt cctttgctgt
     1681 atcaggggtt gtgctgcctg tcaggattgg tataacaggg attcccggga atagatattg
     1741 aataaatggc aattgaattt caacgctgtg ttcaggttca aatgctttgt tgttaaacac
     1801 aaaattgtta ttggctgata gtttttgtac ggttttttta tccacaggta tcgttcccag
     1861 tggcgtggca atggcatcat aaacgggcag ggcaaaaccg gtaaactgtt cataatgaga
     1921 tggccctatt ataatgatga tatctggttt ataagtaact atcgccccat aagcttttga
     1981 cgctacatat gcactatagg tatatcctgc gtgagggact ataatcattg gtgtgtggtt
     2041 tatggcatgg tgtttggcat ttctggtaag gctggaaatt aattgactta gttgctgagc
     2101 attgtcagga taccatgtgc ctgctaattt tgattgtttt gtaatgctat aactttttga
     2161 ggtacattgt aacagaaacg gtataagcaa aatagtgata atttttttaa gcatatcatg
     2221 aacttgcctt tcaattaatt attaatataa tgcataaccg tatacagcac gtcaactgta
     2281 aattgtaata ataatataac ccttacagaa actttcagtt ttctaaaaca attatggaaa
     2341 atattttcaa taccagcaat atatcaaatc agtactacaa agcagtatcg taataaagta
     2401 tctggctatt attccggtta tattattctt gatgataata ccaatcttca tattgatgat
     2461 atgctgggtt ttgctgaggt agtgtatcag gagtggtaat gaaaaacaat gcataatttt
     2521 tagcaaaata acattgtaat gttatagaga tggtgtatta acttcctgct aataaataaa
     2581 ttgatattga ttaatattta ctattgtaat ttttttcttg acttttcctt aaaggtttct
     2641 atattgtctc ttagataccg tctgttcggt agatatttat tatagagtat agatatgggg
     2701 ggcttatgaa gaggtttgct tttttaatac tgttaattgg gtttatgttt gtagcatcgg
     2761 caggatttag tacaacatat gttcttcatc tcaatggaat gtgctcaact cattggctgg
     2821 atggcaagag taaaggtgga gaatcccgtt tagcaaatgc accggggtat gtatctgtta
     2881 attgttatgt tgataacaac aacaccattg catattcagc aagtcaattt aaaacaaatt
     2941 atctggatgt ttactgcaaa ggtagcaact ggtgttatgt tgttaattat agtgctggcg
     3001 atgcaatcgt ggggtatatc aatgccaatt acacaccgca gtggaatatt gcgtatgtct
     3061 atactacagc aggtgcaggt ggcggcagtg aaatatcaat tagaggaatt tctgatcttt
     3121 ttacctgcaa tctagggggg cagttgggtg taagtactat acgcaatatg tataaccata
     3181 atgatacaaa cgggataatc atttaccata ttggcggata taaaggcatt tttggtgctt
     3241 cggctttact acctggtgag gacgatggtg ctgttgccta tcattcatct gctgcttgtg
     3301 tcaattcggg aagctataac aatctgtgca actgtacgca ctggacaaat cacgttatag
     3361 cctatacatg ctcgggatat tatttaaatc attatgaaat gaagatgaaa tttatcactc
     3421 aactgggatg gtaaacactt tagttaatct tttaatatat gcgcggtttt gtaataccgc
     3481 gcttttttta ttgatttact aataatagaa attctttgta gtagactgta ttcaatgtat
     3541 gtatttataa ttcaataagc tcatttcagt atctaacgtt gattatgtgg attccggttc
     3601 aagccgggaa tgaccttgag gtaacatgtc attctgagcg atagcgaaga atcgtgtcgt
     3661 tttatgaacg aatagaatat tcggtactac gtccctttag aatgacacag cggtatgata
     3721 ttttcataag gggagttttt atatatgaaa gttaaattaa ctcatatttc attaataatc
     3781 attgttatta ttgctggtat tatgctgtat ataacacact atgctaataa aaacgatacc
     3841 atcgctgaag aggcagcagg cgaaagttct catggatata gtacggtgct tgaagagatg
     3901 ggactcatat caccgcagca agctctacag gactataaac agctatcgat atatcctcct
     3961 aacagcaggc cattgagcaa ggagaatatt gacctgctta tgcctaaccg ccggtatgaa
     4021 cagccattgc ctattgaaga gggcagcgat atctactatc tctttactgc tgataagtat
     4081 cgagttgttg gcgatgatac cattacattt gtattgattg tgttgcgggg tactaaacct
     4141 gattcagaac gaattccaat agctataaca aaatctgtta taaaaaaggg acttaataaa
     4201 aacaaggcaa aatattatgc agaactatcg cttgcaaaaa atacaactgg tgactatact
     4261 gctgagctga catgctcgca aagtttttca tcagaaaaaa aatcatccgc atactgggca
     4321 gaggttgagt ttaatgctgg caatgaacat gtagaggcag caataccatt tgaatatttc
     4381 cctgaaaaca ccatccccgc aaagtttacc ggtaacttta aagaaaggat tgctgacggt
     4441 tcattgtaca tcgatgttga aattgatgtg ctacggaaag gatactatat tgttgatgca
     4501 aatctttttg atgcagaggg tgaacccgtt gcctatacgg tgcaaaaaca ggagcttcag
     4561 agcgggaagc aaatggtacc gcttcttttc tttggcaagg tgttgctgga caaaaaggca
     4621 aagccgccat tgacactgaa ggatttgcgt ggctatcgct ttattgaagc aaccacacct
     4681 gatccagaat tcatggacag ggagatgatg aaaccgtatg atggcgaata taaaacacgg
     4741 ttacactctt tacagggact atcggacaag gaatttgaag atgcaatgaa gcgcaagcgt
     4801 attgagtttt tagaaaagga aaatacaggt gacattggtg tccaatagtc ggataacaat
     4861 ttcatcctta gcatgttgta tctcattaat ttttttctgg gcgataatta cccttcatgg
     4921 atgtactgtc tcgcacaata caaacaattc aataacgatt gtcattgtca gcgctaaaaa
     4981 ttgtgaatca tgctcaatga cggatgatac tattcaatat gtgcagacac attatcctga
     5041 tagtataata aaaaaggttg atatcaataa tcctgatggc gcgatgtatg tatcaaccta
     5101 tcatctgtgg cgtgtgccgg tgtatctttt tctggataat aaagggtcag agctctaccg
     5161 attggaaggt gaacaaaagc gcagtgattt agatactgca ataaacatag tctcaaagca
     5221 atatgagcat gtaaacatac ttaaaaaata gaattgttaa ccacggtcat gctgtacttg
     5281 ttttaacagt tatttcttaa cgttagataa tgaatcaaga tgccggatga ccgtggtaaa
     5341 acatgagagt agccttcctt tttacaaata ctataagtcc tgcttcatta taacactctg
     5401 gtattgcata tttatatctg cataataata tgaatatttg tattgacaaa taatatattt
     5461 ttaataatag taacacttta ttaatataaa ttattgtaga aggtgcttat gaagcgttta
     5521 tttatattta tagtagcaat tgtattatct ggtacagctt ccgtacaggc tgatgacgaa
     5581 gctgttcaat tggaggatgt cattgtatcc gataccttgt tatatcctgg tgcttatcag
     5641 tcaataaaag gaaacacggt taaggatagt tccaataatg atttaatcag tgttataaaa
     5701 aatcaaatac cttcatttta taccggtgca aacagggtgc tgggttatgg tattgcacga
     5761 agtggcgctg caacgatgtc aatgcgtggt attggcaaat caggatgggg tccaacaaca
     5821 ggtataccta ttctatacaa cggtatggat acaactatgg gtattatggg gcatccggtt
     5881 gctgacatca tgaacatgaa aaatgttgat agtattgaag tactcattgg tccgcaaccg
     5941 gtgctatttg gctcaggagc aatgggcggg gtgatcaatg ttatcacaaa aagacaaaaa
     6001 gaggatggat atactactga aattcagggc tcgtatggaa catggaatac caccgaggat
     6061 tacttgtacc atatgggaaa atttggaaaa ttagattata gtgctggata ccagttccaa
     6121 aaaactgatg gagattggga acagacaatt aacggcaaaa catttacttc tgaatttatg
     6181 cagcagaatg gtactgttca tgttgggtat gcgctttcac ataattggta tgcaggtgtg
     6241 gatagctacg gcatgaagca gaatatacat gatccaggtc cagaaggtat tgatgcatta
     6301 tcagaaatac cgttaaccaa ccagcttaac cttcttgaag aatttgatat tacacgaggt
     6361 ggagttgttg taaaattagc tcatgattat ggcaactata gtggaacatt acaggcagaa
     6421 ggtaattttg gtaaccatga atcaaagcag agcaatactg atattgataa atttgaatct
     6481 gatgacagtt catacgctgt acggttaaag gagatagtta aactatggca ggggaatacc
     6541 gttacaaccg gtgcagaatg gcggcgatat ggcggaacag caaaggatat tgaaaatgat
     6601 tcatattata tgaaagataa atttatgaat gatacatcga tatatgtatt gatggatcaa
     6661 aactttttaa aaaatatttt aagtgttaac ggtggtacac gttacagcta taattcagaa
     6721 tatggagatt atattgcatg gcagggtggt gcagcagtgc gaccgttcaa aaaaacaaaa
     6781 ttttattcaa gtgttgcaag gggatttaag ttccctgata tacggcaggt ttatctgaaa
     6841 ggtatgtatc ctgccataaa tcccaatccg gatcttaaac ctgaaacata taccatggct
     6901 gaaattggaa tggagcaaaa gcttggtgat acaattacaa tacatatggg cggttataga
     6961 atatggtcgg acaacatgtt tgtgtatacc tcgcaatggg aaaatgctga tgaattttcc
     7021 tataatgggg ctgaagcaat gattactgta caggctttaa aatggctatc attcaatgca
     7081 ggttattcat atatcgataa caagcaggaa aatgggtatt taccctatgt tccggcgcat
     7141 aaagctaatg ggagaataac gctaaccata gcagatttta ccatacagct tgatggtgaa
     7201 tatgttaatg agctctatgc tgatacgcaa gggttaaaag agtttgacag ctactttata
     7261 ataaattcaa agatgtcgta tactttttta caaaaatata cagcatttat ttcattgtat
     7321 aatatcaccg ataaagaata ttctaccttt gctgttttgg taccaccact tggggactcg
     7381 tattgtgaat atcccatgcc cgactttcat tttctggctg gattacaggc aacattttaa
     7441 tattaaagta aatcaataaa tcaatataat taataccacc attataaagg gatgcagacc
     7501 agcgcatccc aattttttta tatagatagt tattatatag atagttaatt gtaaataagc
     7561 ttgcaaactg agaagtgttt tattatatta ataacaataa ttatttttat tggttatagg
     7621 ttgtaaatag taaatgagtt taatttcatg ttaaaatttt catggagagg tgctgtatgt
     7681 ggataagaca gccgggattc gcaggttcat tttatccagc aaaccctgat aaactaaaaa
     7741 atgatattca acgatatatt gcaaatgcaa aaatcgtttc tcttgacaaa cccgtagcat
     7801 tcatatcgcc ccatgcaggt tatatctatt ctggtcctgt tgcagcatat tcatatgcac
     7861 agcttaaaca caaacctgtg gatgttgtta taatactggc tccatcacac agagtgcgtt
     7921 ttaatggggc ttcagttatt tcatcaggaa tatatcaaac tccattagga gaggtccaga
     7981 ttgacgaaac tatcgcccag aagcttttac aaaacaatgt atttgaggaa ataaaagagg
     8041 tccatcaata cgaacattca ttagaggttc agcttccttt tttacaggtg gtattgccac
     8101 agtttgtttt agtaccaatt attataggga caattgattt taccacgtgc aaagaaatcg
     8161 gcagcgaggt tgctaaaata ttacatgatg aacagcgaag atttgtcatt attctttcaa
     8221 cggatttatc acattattac cactatgata ttgcaaaaaa aattgattca cattttattg
     8281 aatcacttag aaaatttgac gagctatatc tgtatgaagc cttgcggagc gataaagcgc
     8341 aagcctgcgg tgaaggtccg gtattggcgg gcatgattgc ttcaaaacaa cttggggcta
     8401 caaaggtgga aatactgcat tatgctaatt ccggtgatac tgcaggtcct aaaaatgagg
     8461 tggttggtta tctttctgct gcatttgtgc aatgataatg gaggatgcaa taactgttga
     8521 taatactcat gaaagcctcc atcaaagtaa atatttttaa taagggaaaa gattatgata
     8581 gtactcaatg atgaacaaaa gataaaattg cttacattag cgcgcaaaac cattgaagag
     8641 tatttaaaca aaagaacgct gccaaaagta gatttcaaca atgaatttaa cgatgaagtt
     8701 tttaagcaaa aatgtggtgc ttttgttaca ctgcataaaa acggtgcttt acgtgggtgc
     8761 ataggctatg ttaaaggaat taagacaata ccagaagcag tagttgacat ggctaaggca
     8821 tcagcttttg aagatccgcg ttttgaacct ttaacaaaaa gtgaacttga taaaattgat
     8881 attgaaattt ccatccttac accaattgaa ccagttaatg atatttcgga gatagttatt
     8941 ggaagagatg ggcttattat aagcagtgga ttccgctcgg ggttactgct gccacaggta
     9001 gcaactgaat atggatggga cgttaaaaca tttttggagc atacatgtta taaagcagga
     9061 ttgccgcctg atgcatggaa atggaataat acaaaaatag aaaaattttc tgcaattgta
     9121 ttcagtgaaa aggaattaaa attgcgatat tgttaatagt aataaagtta atagtaataa
     9181 ctattttgtt tttgggggat tttgatgata acaaaaaaaa tgtttggtgt atcattatgt
     9241 atgctggtat cttttatgtt gttttcatgt tcaaaccaaa gggattactc agatattgaa
     9301 aatctgttga accgatgggc aaattcggtc aggtatctta attatgcaga atacactaag
     9361 ttagaagctt atcctaaaga tagcaataca tttaagacta tgtatcaaaa ttattatatt
     9421 acaaatttac aaattgttga agtggatgac aaactgcaca atggcacaga tagtgatggt
     9481 aaaccttacg aatttaaaaa tgtaaagttt attgcaacaa tcataaaccg ccagagcggt
     9541 aaaggtgaac aggtagtgaa tggcgatatt aagttaattc gctatgccac cgggacaaaa
     9601 aaagaaaaag gctggttatt atcaaacagg acgcttattc atcaacaata atatccaatg
     9661 ataaatattt ttaacataca ggtgaaaaaa gaaaaaatca ttatagcttt tattgtaata
     9721 gttatttctt ttttaacagc aacttctatc aagccacaag tccagaataa acaactaata
     9781 gataattata ataaacaggc tatagaatac tataataaaa aagatcttac aaaggcaatt
     9841 attgaatggt taaaaatcct ggaaattgat cccaacaatg aagatgccca gcgcaatatt
     9901 gaacttgttt atgaagaaaa acataaaaag gatatttcat tccaaaaagc taaattatat
     9961 taccggttat cacggaaagt attaccggag gatattgacg ctgcaaaaaa taattccagc
    10021 atagctattg aacattttat tgttgcatac cgcttagacc ctaatgaccc cgaactacag
    10081 attttaaagg atgaacttaa attgcttagt gatgaaatta aacttgaatt agcaaaaaaa
    10141 cgtttatcag aagaaataaa gaaaaaatat tatgctttat taagcgaagc tcaaatatta
    10201 atgaatgaaa aaagatatga agatgctatc aaagtatggg atgaaatgtt acgtttagtg
    10261 ccacttgatg ctgttgctgt tgaaggtaaa cgccaggctg aattagcaat aaacaacagg
    10321 ttaaaatttg aaaaaattat ggctctgatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnna atgaaaataa cccctattac tcaattgttc aaccattaat agaatctggt
    10501 aaaagactgt ataatcaggg taatattgca gagtcaagag agcaatgggt gaagatatta
    10561 aacctattcc cgcagaatag aattgcaact gaatatttat tgagatgcga tttagctaag
    10621 aatcctgagt cattccaaaa atttgccgaa aaaattgtta atgatggaaa aaaattatta
    10681 gctgaaaaaa agtataatga ggcatttaaa aaatttgatt taattgcaac tattgatcca
    10741 caatatcctg aaatacaaaa tttaataaca caatcaaaag ttgaaaaaag aaaaacgggt
    10801 ggtgaggttg ctgatatagg tttcaggcaa cgatataata tggcacttca attatatcag
    10861 cagggtggaa aaaataatct ggaaaaggca ttagcagaat ttaagattat taataataaa
    10921 tatcccgata atatacaggt agctatcatt attaataaaa tagaatcgca attgcgattc
    10981 caggttgcgg aacaggttca gggtaaaaaa ttaacacccc gacagcaaga attaataaga
    11041 gaatactatt ttaagggtat taattattat acaaataatc agtttgatct ggcaattacc
    11101 gaatggagaa aagtacttgc catagatcca gatcatgaaa aagcaaaaaa taatattaaa
    11161 aaatgcttga ttcttttagg aaggtaaatg atgtcaaaag aaaaagcttc agataatgaa
    11221 aaaaataaga aaacatcaaa aaataaaaaa aagaatatta aagatgataa cagtaataca
    11281 attgcaacat ctaaaaaaga agtgcgattt agcctgcgac taaaattttc attggcaatt
    11341 attttgctgg taagcagtat tattaccgtt atgacgtact attttataca gcaagaatct
    11401 gaacttttac ggaaacaaat tttgcaattt gcaaacaggg aagttgaacg tcttgctgcg
    11461 atagcccgtg agtccataag ccagaaagat gagttgccgt tagttggcgc tgtacaggat
    11521 cttaagaatt tagaatttat tgattatgtt tatgtaatag ataccaataa acgtatcatt
    11581 caaaattttg atcctcaaat gaatgggact attattgatg atgatttaac cagtaaggct
    11641 tccaacaaca ctgattctgg aaagccgctg gttgtggtat acagagataa taaaaaaaat
    11701 gttaattttt atgatctgtc attgccacta tttcatacag tacaaacggt aaggataggt
    11761 accgtacgat taggattcag tgaagaaata atattgaatg aaatcagaaa attaactatt
    11821 acaatcctat taattggctc attctttatt gttatctcaa ttgtaggttc gatagttctt
    11881 gcaagtgtta ttataaagcc tatcaagaaa ttgaccgaag gagctgttat tattggtggt
    11941 ggtaatttag atcataaaat tgaaattgaa agctcagatg aattaggtaa acttgctaac
    12001 gaatttaatc aaatgactca aaggttaaaa gttgctcaac aggtggaaat tgaaagccgt
    12061 ataatgcaag aacagattga tttagctcgt gaaattcaag aagggctcaa tccaatgggt
    12121 ttttataata aaagtggaat cgaaataaaa ggatttacac gtgctgcaaa aggtgttggg
    12181 ggtgattatt ttgattacat tgacatagat gaataccggg tgggtgcttt aataagtgat
    12241 gtgtccggaa agggtgttcc tgcttcactg gttatggtaa tgatcagaac agtttttgtt
    12301 tcatatatac ggagggggga tgtacagtgt gctgatgttg tacgggcaat taatgattca
    12361 ctcagtgcag attttgcaat agataaattt gccacactgt tttttatgat ttataatcgt
    12421 tctaataatg agctgtcgtt ttcaaatgca ggccatggcc cacttttttg ttatcgttct
    12481 tcactggatg catgcacatt gacaaaactt gaaggcgtac caattggtat tatggaagat
    12541 gttgactata agcaagctaa agttttgtta aatattggtg atattataat aatgtatact
    12601 gatggcgtta ctgaaatgag gaatgctgac aaagaagagt atggtcaaaa aagattaaag
    12661 gatctagttg ttgccaataa gcaacttaat gccaaagagc tttcagattt aatagtgaaa
    12721 gatgttgaca atttcagagg tgatgtgcca cagcatgatg acatgacact tcttgttcta
    12781 aaacgaacac aataaaaatt tcatgtaaca tacaatccgt taaattagat cattttcatc
    12841 ttcatagatg ctaaaaaacc tatcaagcgt agccagcttc aatatattta atacatcgtc
    12901 atgtatgttg attaatgcaa attttccatt atgggcacgc attttctttt gacctgcaac
    12961 cagtgcgcct ataccggatg agtccatgta atttacatct tttaaatcta cgacaacgct
    13021 ggaataagta ccatcggtta cgctgaataa tgcctttttt aattcgctga cattataaag
    13081 atccacctcc ccgctaacag atataacttt atgttcacca atatccttta attctatctc
    13141 cattaaaagc tcccaaaatg gttttaagtt ttacgtttgc ttgaataata taaaaaaggt
    13201 taatattaat ttcaagcata attttcatga taatggcaaa ctaaatgaaa atttaaccca
    13261 cttaccatat tctgattcaa cccagatttt acccttatgt ttctcaataa ttgatttgca
    13321 tatatacaat cccaagcctg aacctttaat accttcagtg cctttttgct ttaaccgtga
    13381 aaacttctta aataattttt ccatatcttc ttcaggtatt cccacacctt cgttatatac
    13441 tgagaagaca tattcattat tttgttgtgt taactctatg ttaatagtag tattagcctg
    13501 accatatttt attgcattat taattaaatt ggtaactaca atctttagta gatttgcatc
    13561 ccccattatt tttggtttta cctgtatgtt aagctctaat ttcatgtttt ttatattatt
    13621 agtgttttcc ggcatttcaa ctataggctt gataacttct tcatataagt ctatctgctg
    13681 actaaaagat tcaagggaat ccaattccat ctttgataaa tccaggtaat tacggataat
    13741 atcttctaaa tactgacagt tgcgaagtat tgtttcaatg attttttgct ggtcgggaga
    13801 taaattccca aagtatcctt tatacagtgt ttcagccgtt gtgtgaacta cagaaacggg
    13861 actttttaat tcatgagaaa ctatcgccag aagatcaagc atggcagact ctgctttatt
    13921 ttgacgcgct ttgatggcat tatcaacaac attactcaat tcatcaggtg taaatggttt
    13981 tgggatatag tcaaatgcac ctaatttcag ggcttcacgg gttgtatcaa cagttgcaaa
    14041 accggtaaat ataataaccg tgacatctgg ttgttctttg cgcaatgtct ttaatacctc
    14101 catgccatcc ataacgggca ttttgagatc ggtaataaca atatcgtatt tttttgcctt
    14161 agccttttca agtccctcac gtccattggt tgcagtatca atggttaaat cttctcgttt
    14221 gcgtaaaatt ctttgacaac tttttaagat aatatcttcg tcatcaataa ttaataagtg
    14281 taccttgttt tccataatta aataatgctc actgttataa ttatatgaaa tgtttggtct
    14341 atttattgat atgtttgtgt ttatgtcaag caaatagatt gataaagtaa tctaccagca
    14401 gtattccatg atacttatct tttttgtata aaaagggtat caataaaatg tgacaggtct
    14461 aaaaagtttt gtacctcacg catctcggaa caatagggaa tataaacgtt agctatactg
    14521 tcactccaat tccacaaatg ttgcttatca ggattaagcc agtatatatt acgggatcgc
    14581 tctttcattt taataaatga atctaatccc gggtcctgat tattgttacg cgcatcacct
    14641 aaaactaaaa cagttgtttt gctggtaagt gcatcgctgt aattttgaat aaactgatta
    14701 aagctgttac cgtaattact gccccaaccg taggtaaaat tggtatcgct gaatattgag
    14761 tttaatgctc gttccggatc catttcccgg aacaagtcgg taatttcaac aaggttactg
    14821 ataaatgcaa aagaacgtac ctttgcaaaa agcgactgca acgtatacgt caacagcaac
    14881 ataaatcttg aatattggtt aaccgaactt gaaatatcgc acaaaaccac cagttgaggc
    14941 ctgtcaattt tcctatccct gtgaaaaatt ttaaatggaa cgccaccgta ttgaagattc
    15001 ctgcgcaggg ttttttttat atcaaagttt cctcgcttta cccttttttt ccgtaatgat
    15061 atcctgtttt tcatcttctg gccaaatcgc tttattaatt ctcgcatctc ttttatttca
    15121 tcaggtttta gctgatatat gtatttattt aaaagataat cctcacgctg ttttatagta
    15181 ctgcccatgg ttcgctcaaa catgcgtttt ctaatcatac ccataatgac ttcaaatagt
    15241 tggggcgata gttcctggta atcagaatca ttggcaccag cagatgcaaa ttgtgagcgc
    15301 ttgttaaaca acgacaatcc cacagcaccg ccagattcag atgatagttc ttcatcaatg
    15361 aaaatttttg taagatactc gtttgatttt ttaaaaaaag attgaggatg tgattctaaa
    15421 aaattgtcaa ttaatgaatt gagcaacgat gtttcttccg gtgtaaaaat aaaatcttca
    15481 gaatgcgcat gcagagtgta gatatccaat aaagaatcat ttcccttttt tcgctcaaaa
    15541 aattgttcaa aacattgatt aaaaacagga atatcagtat aatctttaat aagagtggta
    15601 gctaatgttt gtttaaaaac ttctttttcc agaattgata tatgtgaaat tgcattcatc
    15661 atatccatta tttcaacaat ggatagatca actcctgctt tttttaaaac ttctgcaaat
    15721 tcaaaaaaaa ctcctttggc cttgtcttct tcaattataa tgttttttat aatattatta
    15781 ttgtggaaca tacattatta gtgaacaata atagaatgca gattcttttt cacaagctca
    15841 atatcttttt catactttaa aataaagttt aatgtatcaa ttgctatatc cctgttcagg
    15901 acttcacagc ccatcaaggt tagtgctttt gcccaatcca gtgtttcgct gatgcttggg
    15961 tcttttttta aaggaaattt tcttatttca tgaattgcat taataatttc tttaatgaga
    16021 taattgccac attcaggaac ttttagttgt attatccgtt cttctaattc ttttgttgga
    16081 taatcaatat aaagatgtaa gcatctacgt tttaaagcat ctgacatttc acgggcgtta
    16141 ttagaggtta gtaatacaaa gggtttgttc tttgcaacaa tagtacctat ttcggggata
    16201 gtaacctgat aatcagaaag aacctccagt aaaaaagctt caaattcaga gtcggattta
    16261 tcaatttcat caatgagcaa taatgaggtt atatcatttg taatagcttt tagtataggg
    16321 cgtgctacta aaaatttttc tgagaaaaaa acatcttctt cgctggtcaa tttatttact
    16381 gcatctttta atgtagcctg atcctcaaga attcttgata atttatcttt caatatctga
    16441 gtatacaaaa gctgcttggc atattcccac tcatacaatg cttttgcttc atccagacct
    16501 tcatagcact gaaggcgtat taattcaaat cctaaagact ttgcaataca tttgcctaaa
    16561 tcagtttttc caactcctgc aggtccttct actaaaacag gtttttccat ttttaatgca
    16621 aggtatacag tagttgctat gtctttggaa caaatatatt tttgtttctc caactcatgg
    16681 attgtattct caacagaggt aaacataata gagcctttgt tttataagtt gataccattg
    16741 ctacaaatat agcataatta tcaagaactt tatataaaaa taattttact gaacagtatt
    16801 tatagcgtgt attcatcatg aaaagtatta taatctaatc ttgttaaata gatttaaatc
    16861 atggagacgt aaagtaatgg aatcaccagt ggtttgtgga ttttccatac agtaattgga
    16921 aattttttga gtaacagaca atactcgtgc tacgtcaccc aatgtatcta tataattatt
    16981 agaaaatgca ttttctaatt gttgacgggt aaatgttttt tgtaatgcca acagattttc
    17041 aacttcgtct atgagttcat tctgatcagg catggttata ttttttgcca ggtaacataa
    17101 atagcctatg tatgcttcac ctatcaatgc acgctctacc atgccccatt tttttaaaat
    17161 tttacgtaac tccttatcct tcaccggact gcattgtaaa gcgttgcagg gaaaatcgtc
    17221 cagagtaagg gtagcatggt taattgttgg taaaattgac atgtcaagct gttgcaatgc
    17281 attttccggg agctggtatg tttgtatata tacaattttt gtaaatttat cttcaccagt
    17341 ccttcttgca gtgacaaaaa gtaaatctgc atctttgccg ccagtaatat attttttatg
    17401 tccggatagt gaaagcgtat tgttatcgat ataccgtgct gtcattcttg cagtatcagg
    17461 ttcggtgtga gccatggcac ctattgtaac cggtgttaat gctggtattt cccatggttg
    17521 taactggttt atatcaaagt aaacgcatct cattaatact gattgaacaa tccataataa
    17581 agcaaatcct aaatcgccac cgtgcaatgc tacatcagtt gatggcagtt ggttgaagtc
    17641 taataaacag tgtgatgggg gataatcgct tgcaaatgct atccatgatt ctttaaataa
    17701 agaatgattg cgcactcggt caacataagt agactctacc atatttaagg aactatcgcc
    17761 catattattt tctttttagg tttaataacg cttcaatttt tgtttgtaaa ccaaaaaggt
    17821 ttataaagcc tgttgcgtct ctgtggtcat agagagcact atcgtcaaat gtagctatgt
    17881 ctggctcgta taaagtcacg ggtgatttgc gtcctacagc catgcagctt cctttaaaaa
    17941 gttttacctt aacagtgccc gtcacattct tctgagattc attgataaat gcatccagtg
    18001 cctcccgacg ttttgtaaac cacaatccgt tataaacaag ctcagcatat tctattgcnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt taactcaaag cgtacctgat
    18181 cattgccttt tcccgttgct ccatgacata ctgcatcagc gccttcctgt aacgctatct
    18241 gtacctgttt ttttgcaata acagggcgcg ctatggatgt gccaagcaaa tatctatttt
    18301 catagattgc atttgcttta atacagggga aaataaaatc gcgaacaaac tcttctttta
    18361 aatcttcgat ataacatttt gatgcaccgg tattgattgc tttttgttcc aatccattga
    18421 gttcctcttc ctgtccaacg tctgcagcaa aacagatgac ctcgcattta tatgtttcca
    18481 gcagccactt aacaataacc gaagtatcaa gtccaccgga gtatgccagc acaacttttt
    18541 taatactcat actgtacctc aatagcaaac gatagtaatt tacataattc ttaatagtat
    18601 attggtacgt tgaagtatag ggcattatag tcaagctaaa tattgatggt tatagtaaaa
    18661 actgcacaag ttcatgtgaa ttaacaagca ttgcaaacag gcgatatttt tttgaaacag
    18721 cttcgcgtga attagcacgt gccaggagag ttaatcgtcc tttttccatt attatatttg
    18781 atctcgatca ttttaaaaat atcaatgata cctatggaca tcttaaaggt gatgatattt
    18841 taaaaaaaaa tagcacaaaa ggttgtatca cagctacgta ccctggatat ctatgcacgg
    18901 tatggtggcg aagagtttat catctgtttg cctgagacaa tgccagatga tgctgtgata
    18961 gtagctgaaa ggatcaagaa tatagtggag gaatgtacat ttaacgacaa tgaccattta
    19021 ataacagtaa ccataagctc agggattgcc tcattggcgg atatagacga agagctggaa
    19081 atgatcataa aacatgccga tgaggcatta tatcaggcta aacaaagcgg aagaaaccgt
    19141 gttgctgttt atagaaaaaa tggatacaat taagtgcagt actattttgc cattaaagca
    19201 cataaaatag acttttgtac atggagcctg ttctcggcct gatccaatac tatggattgg
    19261 ggtgaatcta tgacctcatc ggtaatttct tcaccgcggt gggcaggtaa gcagtgcatt
    19321 actttgcaat tgggagcaca tttatccaga atcttcattg ttattttgta ttttgcaaaa
    19381 ttttttctcc gtcgagacgc ttccttttcc tgccccatgc taacccacac atcagtataa
    19441 agataagtag cctgttcaac cgccagtgca atatcacttg tcagagtaat aacactgcct
    19501 gattttgaag ctattgaaaa agccatctca actacatttt ttgcaggata atatccttta
    19561 gggcatgcca gcgcaaagtt tacacccagc atagctgcgg taagcatgag cgattgagca
    19621 acattattac catcaccaac aaatgccaat ttctcatttt taaaattttt attgtgctcg
    19681 taaattgtat aaaaatcagc taatgcctgg caaggatgaa acatatcggt tagcccgttt
    19741 atcaccggta tggttgcatt ttcagctaat tccagcgctg ttgcatgagc ataacatcgt
    19801 atcatgattc catctaaata gcgtgataaa actctagctg tgtcaccaac tgtttctcca
    19861 cgtcctaact gtatatcgtc tctgcttaaa aacaatgcat aaccgcccag ctgaaacatt
    19921 gcaacctcaa acgaaactcg tgtacgggtt gatgtctttt caaaaatcat ccccagtgtt
    19981 ttaccctcaa gaaaattgtg tgttttgcca tggttaagtt cgtctttcat cttttgtgta
    20041 tattcaaaga tagcaataat ttcttcttta gaaaagtctg taatactgag caaatctttt
    20101 gtttttacct taggaatttt accaatcatg ctgttactcc tgcaaaaata ctatcaagga
    20161 tagacaaccc ctcagtaaca agttttttgt taatggtcaa aggcggcatt attctaatta
    20221 ccttttcggc agtacagttg ataattaaac ctttttttaa tgcttcaaca acaatgggtt
    20281 gccctgccat tgaaagctca attccaatat gcaaaccaat accccgcaca tcaataataa
    20341 gggtaggata tttttttttc ataagcgata gtttctctac aataagtttg cccatggtat
    20401 tgacattgtt gagcaaggat tcttttttgc aatgttccag aacagttttt gctgctgcag
    20461 cagccaggtg attgccgcca aaggttgtcc cgtgtgttcc ctttggaaaa aattccacca
    20521 aaatattttg agcgtgaatg gcaccgatag ggacaccacc tccaagacct ttagcaagcg
    20581 atatgatatc aggctgtatg tcataatgct gataggcaaa catcttgcca gtacgggcaa
    20641 tacccgtctg tacctcatca attattaata aaatattgtt ctctttacat aatgagctga
    20701 tttcttttat aaagtaacta tcggcaactg ttatgccacc ttcgccctga acaagctcgg
    20761 ttatcactgc gcagattttt cctttattgg cttccagtgt ctttttaaat tgtgtgacat
    20821 cattaaacgg cacatagata aaaccaggaa ctatcgggcc aaaccctgaa cgaatctttt
    20881 cctgagcagt tgccgacatg gcaccaaacg ttctgccgtg gaatgagttt gtgaacgata
    20941 tgatatgata cttatcattg gattgggata ttccatattt tcgtgctaat tttattgcag
    21001 cttcattagc ttcggtgccg ctgtttgtaa aaagggtttt gcccgggaat gatgtttccg
    21061 aaagcaattg agcggcttct atctgctgtt tgttgaagaa ccaatttgat gaatgcataa
    21121 tagcatcaat ctgtttatgt agagcgctat tcaatgccgg gtgattatat cccaacgaat
    21181 tgactgcaat cccggcaaaa aagtcaatat accgtttgcc atcctggtca aagagttcgc
    21241 tccctttccc ttttataaaa caagccggta accgggtgcc ataattttga aaaagatatt
    21301 gattatcctg ttctatcaat gtttgtaatt tttttgatac tgccataaag tatatcccat
    21361 aaaaaaaaat aaatcggttt gacccgattg ctcactcaat atactatcat tctataatgt
    21421 caatatattt tggtaatttt tagtaaaaag ataaaatttt attaaaatga tttaaaaatg
    21481 ggcttgcata taaacttatt ttatagtata ctatatgatg aaaggatgtg taagtgtttg
    21541 atagttacta tggaagatga actatgatat gtgaacggtg taaactaaga gaagcaacag
    21601 tccatttgac tgagattatc aggaatgaaa aaactgaagt tcatttgtgt gataactgtg
    21661 cccgcgatat gggtctaagc tcacacggcg gtggtttttc tcttacggtt tctgatatga
    21721 tgtcctttct ggatgtaaag gatatgaatg atatgaatga tatggttgat agtaacacct
    21781 gtgccacctg tggatggaca ctcatagatt ataaacgaac agggaagctg ggttgtgcag
    21841 attgctatag ctcatttaag gaagctttaa caccaatctt atcaggatat catggttcaa
    21901 catttcatat tgggaaaatg ccactgaaat atatagagca tagaaaatca aaggtaatgc
    21961 ttgattcaat gcgtttgaaa aatgttgcaa cgttatcgct tcatgacctt gaaaatatgc
    22021 ttgaagttgc agtagaaaat gaaaattatg aagaagcagc aaaattacga gatgagataa
    22081 taaaactgaa aaatcaacag ggggcataat gtttgaacag ttgttggaac aatcaccctt
    22141 ctgggcaaga ataggtcctc tggcagatgt tgctatgaca acccgtgtaa ggtttgcaag
    22201 aaatttgcca tcgttacctt ttgccactaa aatgcgggaa tctgatataa ccatactgga
    22261 aaccatagtg cgacaggctg ttattgcttc aacgttattt caaaatactc aatttgttaa
    22321 tctgcgtgat tgcagttctg atgaccgacg ttttttatgt gaacgtgata tcatcactca
    22381 tgagatggaa ttaagtgatt acagctctgt tgtttttgat catcagcaaa atttttccat
    22441 tcttataaat gaagaggacc acatacgtat acaggttata aaacctggat tacagatgct
    22501 ggaagctttc aaattagccg atgaaattga tgatgaatta aacaagtaca tttcatatgc
    22561 tttcaatgat gatattggat ttttaaccac ctgtccttca aatgtgggga caggattgcg
    22621 tgtttcagca atgttgcatt tgcccatgtt gacagcaaca aaaaatatgg gtgaggttat
    22681 taaaattgta cgtgaatatc aggctcagat aaaaggaatt gtccatgatg caacaaaaac
    22741 agtaggtagc atatatatcg tcagcaataa agcaacatta ggaaaatctg agatagatat
    22801 tatagaagaa gttgataagg ttatcagtat tattattgat cttgaaaata atgcccgtga
    22861 agaatattat cagcatcata aagatatgct ggaagatatg atctggagat catatgcaat
    22921 tgttgcattt tcaaggacaa tgaattatgc ggaagctatt gagcatttat cgcttattcg
    22981 gctgggtgtt gtattatccg tattaaaaaa tattgaactt tctgcaatta atgatttaat
    23041 gatacatata caggtatcac atcttcagaa aatagcagga aaaatttttg ccacatctga
    23101 agaaggtgat tccttcagag cagcatatct tcgaaagaga ttcggagata aggagtaaaa
    23161 aaagatgttt gactatacca agaggtcacg aaaagtatta gaagttctgg cacaggcaga
    23221 agggcggcgg ttaaattctg atgctttagg gccggaacat attatgcttg ccctgttaaa
    23281 ggatgatgac tccgtcgctg cacgcatatt gaaaaatatg ggagtaaatt ttgaaatact
    23341 acgcagaaat atagagcagt caattcggag gtcaggttct acaataatac tgggcaatct
    23401 tccgctgagt gctcgtttta cccggattat tgaaatagca aaagatgaag ctcgtaaatt
    23461 gaaaaacaac tatgttggta cagaacatct tttgctttct attttcaggg atggaacatg
    23521 ctctggcatt gatacgctta tccgtgcagg tatagattat aatgttttga aaagtgaagt
    23581 acagaagata ttgggtgtta ctcctgatgg gagcacacag caaaaaggta atgataaaac
    23641 aaaaacacct accctggatg agtttgccat tgaattaaca cgattggctg ctgaagataa
    23701 attggaccct gtaatagggc gtgatactga aatagaacga gtaatccgca tcttatcccg
    23761 taaaacaaaa aataatcctg tactgattgg tgaagctggt gtaggtaaaa ctgcaattgt
    23821 ggaaggatta tcacagcgta ttattcatca tgatgtgcct gaacctcttt ttaacaagag
    23881 agtattatca ttggatttag cttctgttgt agctggtact aaatatcgtg gcgaatttga
    23941 agaacgttta aaacgcatca tgaaagagat aaaaggctcg gacaatgtta tcctttttat
    24001 tgacgagctt cataccatta taggtgcggg agctgctgaa ggagctatcg atgctgctaa
    24061 tattttaaaa ccagcgctgg cacgtggtga gctccagtgt attggagcaa caacactcaa
    24121 tgaatatcgt atatacattg aaaaagatgc tgcacttgaa cggcgttttc aaacagttat
    24181 ggtagatgaa ccaacggctg atgaagctgt tgaaatttta tttggattaa aagagcgcta
    24241 tgaaattcac cataaggtgc gctataccga tgaagctatc aggaaagctg ttctgtatgc
    24301 aaacagatat atacatgata gatatttacc ggataaggca atagatgtta ttgatgaagc
    24361 aggttcaaag gcaagattgg aaaactgtga aaggcctgtt gatattgaat tattagaaca
    24421 ggagattgaa gagcttaatg atcgtaaaaa taatcttgtt aaggctcagg aatatgaaca
    24481 ggcagcatca atccgtgaca caatcattga aagaaaagct cttctttcac agaagataag
    24541 cgattggcag cagcgcatca atgactatgc agtggtagtt gatgaaaaac agatagctac
    24601 cgttgttgca caatggacaa aaatacctgt agaaaagctt gaggagtccg aatcccagaa
    24661 attattacag atggagcagg agctccataa gcgaattatt ggacaggata atgcaatcaa
    24721 tgtcatcagc agggcaatac gacgctctcg cacggggctg cgaagtgctc aaaggcctat
    24781 tggttcattt atttttcttg gccctactgg tgttgggaaa actgaattgg ctaaagcatt
    24841 agccgagttt ttatttgatg atgacgatgc ccttatcagg tttgatatgt ccgaatttat
    24901 ggaaaaacat tctgtatcgc gtttaattgg cgctcctccc ggatatgttg ggtacgaaga
    24961 aggaggacaa ttaactgaaa aggtaaaacg caggccatat tccgtgatac tatttgatga
    25021 aattgaaaaa gcccatcctg atgtattcaa tatattatta caggtacttg aagaaggcga
    25081 acttaccgat aattttggtt ctgtaacaag ctttgtggat actgttatta ttatgacctc
    25141 taatgctggt aatcgtgaat tccaaaagat aggtaagatg ggatttaatg aaatcaatgc
    25201 caatcaaatt caattccaga atgttgacga agaagttaaa cgattattca gtccggaatt
    25261 tttaaataga attgatgcag ttgtccattt ccataagttg aatgagcacc atatacggca
    25321 gattcttgat ttattattac aggaggtcaa tgaacattta acagagcagg gcattgaact
    25381 gttagtatcg cataaagtaa agaactatct tgtcagtaaa ggttttaatg acaaatatgg
    25441 tgcgcgatat cttagaagag ctgtgcaaaa cgagattgaa gacagattgg cggttgagat
    25501 attaaatggg aaatttaaag gatgccataa ggtgtatgct ggcatcaaga accaaacaat
    25561 ctattttaaa cctgagaaaa atggaacagt tggagatata aatactcctg aagttgttca
    25621 ttataaataa aaatatgttc tcatttcatg aaaaaatcac aaaagaaaac ccatggacgt
    25681 ttaaaccata gattcaaagg ttcaggacat agaatcagtt atagtgatgt tatacataaa
    25741 atcaaaaata gatttttttt acctaaattt ttaaagtcaa taaaaaagta tggtatatcc
    25801 ggattttcag gcggcaataa ggttaccctt atcaacaatg gcgatttatt ttttgatgaa
    25861 ttatttgctg ctattgatag tgcaacatta agtattaatc ttgaaacata tatttttaat
    25921 agtgatgata ttggctggaa agttgcacgt aagttagcag caaaggcaaa gaaaggtgta
    25981 gatgttaata tcatctatga tgctgtgggg tgcattaata cttctggcga gctttttgaa
    26041 tatttgagaa aaaacaaagt tgaattaatt gagttccatc ccattatacc ctggcgtaaa
    26101 tatttcaatt tatatctacg agatcaccgt aaactcatgg ttgtagatgg cagcacagca
    26161 tttgtaggtg ggatgaatat agggaatgaa tatgccggag aaaaatatgg aggtgaaaat
    26221 tggcgggata ctcacctgaa aattgaaggg ccagcagtac gtgatttaga gtattttttt
    26281 ctggaaaact ggcatagaca gggcggcgct cttgttgata tagccaggta ttttcctgtt
    26341 attaatcata aaggtgatac tattgtattg gttttaagca gttattctcg taaaaatgta
    26401 aagccgattt accagacata tttagcagca attgccaatg cacaaaaata tatctatata
    26461 gcaaatgcgt attttgtccc tgatagtaaa attagcaggg cattgatcca tgcagcaaag
    26521 agaggtgtag atgtcaggat actattaccc tcggttagtg atatcccatt tgttcaatat
    26581 gcaagcagat acctttataa acgttttctt aaaaatcata tccgtattta tgaatatatc
    26641 ccaacggtat tacatgcaaa aaccgctgtt atagatgata tatggtcaac agtaggttca
    26701 acaaatttag atagacgcag ttttaatgct aatcttgagg ttaatgctat tgttcttgat
    26761 cagaattttg ggaataagat gaagaaacag tttttaaaag atattaaaca aagtaaagag
    26821 atagtatttg agcaatacca gcgaagatcg tctacacaac ttataaaaga atggttttgt
    26881 taccgtttca gatacctgtt ataatataat gattaaacat ttttatacta ttttatactg
    26941 tgattaaaaa ccttccacaa taggtgcaca ctgcaaggtg ctttttagat ttaatctttg
    27001 ccatttcaac tggcggtata accatgtggc atccaccgca ggcatttttt tccagaatgg
    27061 caaaaggatt ttgacatttt tttctcatac gttcataata tgaaagtgag cggttatcaa
    27121 taaatttccg taattcatta atctttcttg ttaatgcttc ggatgtatcg ccggtatcat
    27181 acaggcggta aagctggtca agccgtatga gcaggtcaag gtcagtggaa ggaatgccag
    27241 aaagattctc aatatgttca tcttccttat tgatttcggt tagcgattca aaataaagtt
    27301 ttgcttcttt tgttagtatt ggatatattt ttgacggtgt tcgtgcttca tcgattttac
    27361 ttcgtttctc tgagcttgaa aatattcgcg ccatcatacc aaccaggtaa agatacttgg
    27421 tgctgagtga tgtgggaaat atgctgagca tgacatattt aaccggctgt ttatcaaacg
    27481 attcgtattc aattggttca ttacatattc caaatacagt aagcaatttt ttgactgaat
    27541 cggtgcgaac atgcgggatt gcaaaaccat taccaacacc ggtattttcc agtgtttcac
    27601 gatgtatgac ctgtgtataa tacctgtcgg cattatccag tttccctaat tcctgcaact
    27661 tgtgaattaa ctcttttatt accagttgct tttctgtact cttaatggaa attagatgtt
    27721 ctttgggtaa tagttctgat agatccattg ttttaaataa tctcctttat aaattaattt
    27781 ttttatatgg catatccttt ttttattggg aattccataa tataattgta tcataaaaaa
    27841 ataatattac taaaatcaat acattattat aaaatatttt ttgaacacat ccatttttta
    27901 taattgttat tatctatgaa cagtgctcag gtgcgtacat ttggcagtaa tgacaattga
    27961 tgaattttta ttatcaatat cgatgcattg ctgtgttaat gcacatcaag cattgtgaat
    28021 agtttgaacc aatggatata atcggaatgt accggtaaat agcaccatag tttttccagg
    28081 gctttttttg gcgatagtta ctggtaaatg tatatcatca gtaatgatga gatgatagtt
    28141 tttgataact tcatcatcag ggtaatagct tctgctgtca ttgaggggga gataatatac
    28201 tggatgctgt gtatagcgct gtatgatagt tagcaaagag tggatatcct tgtcgttcat
    28261 aaagcttaca caaaatatac aatcataccc ggaataggtg tttatcaggg cttctactgt
    28321 ggtttgcatc gcttccggat tgtgtgcaca gtcaaacagt atcaatggat cttcaccaaa
    28381 caactggctc cttcccggga tggttatggt tgatagagct ttgattatat catcagtgac
    28441 tatcgcccaa taataatttt ttaaaagtaa aactgcagct atggcaaccg cagcattgta
    28501 tgcttgaaag ctcccagcta acgggatggt gacattgcta catttttgca aaccgccaaa
    28561 cagcggatga ttgcacacaa catcacatgt taacacggga tgatttgatg ttattgaaca
    28621 gtgaatattg gtattaaaca aaataatggg tgcattgttt aatgccgctt catgccgcag
    28681 tgttgcaagc gcttctggat gctggtgcgc aataacaaca ggtgtgtttt gttttatgat
    28741 acccgctttc tggtatgcta tttgttgcag agtatttccc agtattgcag tgtgatcata
    28801 cgcaatgggt gttataacac tcacaagagg agttacaaca ttggttgagt caagccttcc
    28861 gccaagtcct gtttcaataa ctgcaatatc aactgagttt tgaaagtaca aaaatgccat
    28921 ggcggtaaat gcatcaaaga atgtaatgtg cagattatgc tttgtacata gttgttcaat
    28981 gatagtgctg aaatgtgcta ttgtttcatc agctatttca ttaccattga cagtgatacg
    29041 ttcattatac cgcagaagat gcggcgaggt ataaagtcca acacggtaac ctgctgcctg
    29101 caacatggca gcaatcatat atgccgtgga acctttacca ttggttccgg caatatgtat
    29161 tgttttaatg gctgtatgcg gattatgaag caggctcagc aatgtaacaa tagcatcggg
    29221 tgaatagtgt acatagcgcg ctgaatgaac cgattcatta ttcacaagcg attgtaaaaa
    29281 aggcggcagc atggttactg caatgcctga gttagtgcgt tatacataat ttcaaatggg
    29341 gctggttttc ccgtccatag ctcaaactgc atacatgcct gataaagcag catatattca
    29401 ccggttatgg ttttacaacc tttattacgt gcttttttta tcaacatggt ttcacgcggg
    29461 gaataaatga tatcaacgat tatgtgatgg ggatgtatac aatgttcatt aatggggctg
    29521 gtatccgcat gcggtgacat gccaacggga gtagtgttga tgatgatgtc aaaaggcaat
    29581 gttgtatcag ggtgcaaatc atgaagtaaa cagctattga cagatatacc atgatgctgt
    29641 agatcatgta ccagatgcat cacattattt tcattccttc cacagatgat aatatgtgca
    29701 ttgttgtgtg ctaatgtgta ggcgatagct cttgcggaac cgccgttgcc aatgattaaa
    29761 acatttttat gtacataatc aacagcatga tgctctaacg ctttgatgag accattgcca
    29821 tcggtattgt acccttttat gttgccattg acattgatga gagtattaac cgaaccaatt
    29881 tccagtgcca gatcgtcaac ctcatcgcat agagatgcca gttcaacctt aaacggtatc
    29941 gttacacttg ctccctgtat ccccaatgtt ttcattgctt caattgcttt ttgtgcagac
    30001 tgtggttgga atgcaacgta gattgcattt atatgaagat gttcaaaggc aacattgtgc
    30061 atgacaggtg acaatgaatg ccttactgga ttgcctatga tacagtatac cgttgtattt
    30121 gcatctggtt tcacggttta tctcgttcaa taaagttggt tgtaaaatca gtacgttcag
    30181 ctactataaa aaagccatct ttgtcaacct taaatccatt gtgataactg gttaccataa
    30241 tagcttcaag cgggttcatg atgagctgat gttcccagtt tccttcacgc aatagcaagc
    30301 cacactgtcc attgacacaa ataaaaagtt cagggcaact gtgcttttct aacctgtcaa
    30361 tttcatgggc actgttgtat tccggatggt agataccaac gcgccataga ttttcatcct
    30421 gagcaactac attccagcgt gtatcagcag taagtttaaa aatttcattg tagttcatgg
    30481 agtacactcc tcagagcata taaaaatgtt tgttgcaata gatagctgtc acaaacgatt
    30541 acaatttaca atatgaataa acaacaaact aaaagaagta atcataaaaa gttaagaaag
    30601 cagaacacat aatcaaaaat cagtcaagct aaaaggattg tcaatgggca aacaccatgc
    30661 accatcacca tgacagaaaa gcagggtaag actatagttg tatagataat agtaacaaca
    30721 agaactgcaa aataatgtaa taggttttac aaatttttct tgaaaattat ggtgtaccta
    30781 ttacctataa actgtgattt cacctatact atatatagaa ctcatttatt gtagtataac
    30841 tgttgatata ccatgacatg gaatatggat actgcatcat atattaataa tgtgcttacc
    30901 atagctggtt ctgactctgg cggtggcgcc ggtatacagg cggatgtaaa aacaataacc
    30961 gctctgggta attatggtat gacgattatc acagcaatca ctgcacagaa tacattgggt
    31021 gtaagtgatt atatgccggt acctcttgat atgattcaaa aacagtttga tgcagtagca
    31081 tctgatatcc ccatacatgc ggctaaaact ggtatgcttg ccagctctga gattgttgca
    31141 ttggtagcat cgaatataaa gaaatattcc ataccgtata ctgttgttga ccctgtcatg
    31201 gttgcaaagg gcggatcaac actgctttct cacgatgcca tcagtgctgt gattaatctt
    31261 cttttaccgg tggcgcatgt tgttactcca aatctccatg aggctgaggt tttaactggt
    31321 ttaaccatac aaacaataga tgatatgaaa aaggttgcaa agcaaattca ttcatctggt
    31381 gcgcgatatg tcgttataaa aggcggccat ctgaatgata gtgccgatga tgttatttat
    31441 gatgggaacg actataccat tttgcagggc acaaaaataa atacacaaaa cacacatggt
    31501 acaggatgta ccttttctgc cgcgattgca tcatatttag ctaatggata caacgccata
    31561 gaagccatag caaaggcaaa agcatttatt acaaaagcaa tacaattttc attagcattg
    31621 ggtaaagggc atggtcccac caatcattta gttgatattg ttaattataa ctataaagaa
    31681 caagttatac atgaggttga acaagcattt tctgttattc aggctgcaca gtgcggcgat
    31741 atccttgtga gcggttcatc caatattggg tatgcccttc cgtatcctat aaatcaaact
    31801 gattttgctc tctttccggg aggtatcata acccatcagg attcagtctt caagataaaa
    31861 gacgcagcat gtaatgttga acatccgctt gctgatatga tgcagaaaat taaaggtaac
    31921 tatcgcacag ctataagtat aaaaaatagt aatgccatca gggcgatagc tcatgctatg
    31981 gatgtgccgg ttatacaggt gactgctcct gatgaactta tgaacacggg ttttgataaa
    32041 tcgcagatta tgattgttgt tgatgatgat ccgttgttat atattgctgg acaaaaccct
    32101 cttgatgtag cacagatagt ggttagcata aaaaggagat tgcatgaata tacctcataa
    32161 acagctattt gattttctgg ataatgagtt tcctctaatg tatcaggagg aatatgataa
    32221 ttccgggcga cagatagctt ttgatgaaag taatgttacc ggaattctta tagctatgga
    32281 tatatcccca cagatcattg atgaagccat tgaaaaaaaa tgtaattgta ttataacaca
    32341 ccaccccatg tttttccgtt cattaaaaaa tattaccata tccagtgttc atggtttcat
    32401 gacaatacgg ctgattcaaa atactatcag tgtatatgca gtgcatacca atttagatgc
    32461 actatgctgg gatattttag caaaaactgt caggcttaac gatatcaaaa tgttatttcc
    32521 tgataagcgg tttaatggaa ttggttttgg tgccattgga tgtatgcagg atgaaatgtt
    32581 gttatcgcaa tttattgagc agttgcagca tgaacttaaa accatggcaa ttatttatta
    32641 tggcaatctg gataaaaaga ttaaaacgat aggtgctttt aatgggtcag gttcaaaaaa
    32701 gatcaatgct atcatacaat catatcccgt tgattgcatt gttaccggtg atgttggtta
    32761 tcatgatgca gtctatgcca ataatcatga tgtggcggtt attgatattg gacattatag
    32821 ttccgaaaaa cccctccttc attttttaca ttcaaaacta tacaactact tgacaaatgc
    32881 catgcattgt agcgatgttc gtttaataat ttcagaaaaa gaacaaaatc ctatgcattg
    32941 tggtgtacaa aatgggaaat gagatatatg taatgttaga gcttcaggct atatggaata
    33001 aggttttaga ttctaaaact gagattgcac gttcacgtca tgcaattgat ttctggcgtg
    33061 atgaaatgaa gagcattgga gccaggatgt ctgatttgcg ttcaagagtt gaccagctta
    33121 aattgaaaat caaagaaaag gaaatggaat tgcatgatgt agaatcaagg attgaattgc
    33181 tggatagcag aaaaaattct ttaaaatcgc aacgcgagtt tgaatcaatg caaaccgagc
    33241 tggataccct ggctaaaaaa aaagatacta ttgaagaaca acttttacag tatatggaag
    33301 atgtagagca gatagaaggt gaattagaga ttttccatgt ggagatgccg aaacgtttgc
    33361 aacaaattga aaatgatgtt cagcagttac aaggtaaatt agaagtaatg caaaaacaag
    33421 ctgaagataa tgcaaaggag tttaataaaa ggatatcaga gcttccaccg gatttacgta
    33481 tgcgttttgc aaaggttatt gatacaaaag ggaatgcaat tgtaccactt gaagatggca
    33541 tttgtatggg ctgtcatttt caggtacctg tgtatgtaaa ggatgaggtt ataaaatcaa
    33601 gaccggcaac ctgtactaat tgtgggaggt ttttgtatct aaaaaattag ctataatatt
    33661 gtaaatggta tcttttttgt acttgttttt aagctatttt ttattgctgt aattaaagtt
    33721 gacgtaatgt aaaaataatt atataacata gcatatatag aattattata aaagagataa
    33781 tacttctggc atttattgtg aacggcaaaa gtaataaaag tcattttgga aaacggaata
    33841 agcatgacac gtaatctgaa agagaagctt catggtttat atggaaaatg aaaattacac
    33901 tatatagttt aagggaaaaa taatgttact gagtattgat agagtattgc agttattagc
    33961 tgaaggtaag agtatagaaa agattgcaga gcttgctaat gttgatagta gcgatgtatt
    34021 agctataatt gaagaagcac gaaaaattgt taatcaattt gataagcaaa aatcaaaaag
    34081 caagattatc ataaaaaaga aaaataatat taacactcag atcccaatgg agatgaaaga
    34141 aacggatgaa gaactatttg atggatttga agtgttatca accataccta ctgaatcagt
    34201 tttaacaatg tatgttgatg gcgcatcaaa aggaaatcca ggaccggctg gaataggcat
    34261 agttatatat aataatgatg accagtttgt tgggaaagta tcgtcatata taggtaaaaa
    34321 aacaaataat caggcagaat acattgcttt aatacgggca atgctgatag caatttattt
    34381 taaaactaaa actttaaaaa tacgcagtga ttctgagctt gtggtgcggc agcttctggg
    34441 ggaatatcag gtaaataact cacaaataaa aaagttatat gataaagtaa tggaattaaa
    34501 aaaacagata ccaaacttta agattgagca tgttatgcgc aactttaatg aaagagcaga
    34561 ttttttggca aagaaggctg cagataaaaa aatataaaat aataatagaa agataaatgt
    34621 tatatagtat ttatatataa gatggaaaat gtgtatacca aaacagctga actatcaaaa
    34681 aaaacattat tgcatctttt taatatcact gatgtaacag cagacctatt attactacga
    34741 ggtattgaca ctccctataa ggcttatatt ttttttaatc ctgaactcat taactgttat
    34801 agtccatttc ttttcaatag aatcaatgaa gccattgtat taattaaaaa gtgcatacag
    34861 caaaaaataa agtttgggat tttttcagat tctgatattg atggattgac atcattaact
    34921 attgtatcac acctttttga tctactgggt gcggattatt gttatcgata tccagttgga
    34981 gaagagctgt atggattaac catccccata attgatgaat ttagaaagcg cgatgtacaa
    35041 tgcatcataa cgcttgacag cggaacacgg gatgttgaag aaattgcata tgcgaaagct
    35101 catggtatgg atgttattgt atgcgatcat catgaacctt cggatgaact tcctgatgct
    35161 gtaataataa atccaaaatt gcatacttgc agttatcctt ttaaagaact tgccggtgtg
    35221 ggagttgcat ttaagttatg tattgctttg ttattaagct ataccagata ttatgaacag
    35281 tatgtcatta tttgtgcagt tgacagtaat gcatatcatt ataaagtaat gcacagaggc
    35341 tgcactaaag aatgtggtgt agtagatgat atatctgata tacagcatat tataaataaa
    35401 tataccaaca cgatactact acattatgat gtagcggcag tcaatgcatt acagcatgca
    35461 aaattattca atttacaaga ttattcagga gaactatcga acaaaaaaaa tcttttgaaa
    35521 attgaaccag aaaaagtttt tccttctgca ttggattttg ctgttacagt ggctcagaaa
    35581 atgcagttcc agcaatcaaa aaagatgatg aaatttataa aagatgtggt gccgctggta
    35641 accatgggaa ctatcgcaga tatgatgccg ctggtggatg aaaacagaac actggtaacc
    35701 gctggtattt cattttttaa taaaacgtat catactggta taaacgttat tcgcgagcat
    35761 attgcaaaac ccattaatgc agatgttatt ggctgggata tatcaccgct tttaaataca
    35821 cctggtagat ttggaaaaac agaattggct gcaaaatttt ttttgatgga taaatcttcc
    35881 gaagtaaaag gtatattgga tgaaataata caactcaatg ctgaacggaa aaatattata
    35941 caaacactgg ttaacaaatt tagcgaaagc tatagtgcta acgctaataa aaaaatcatt
    36001 atgatacaat ctaatgagat tccggaagga ttgaccggta ttattgcaaa cagaatagct
    36061 gatcaaaccc tgttgcctgt tatagtcata gccaataaaa ctgacagcac tgttgttaaa
    36121 gggtcgggaa gggtaggtaa tcaccttgat tttttttcaa ttgtttccca atttaaacat
    36181 atatttgaaa aattaggcgg tcatgagcag gcatttggat tttcaataca taaaaaaaat
    36241 atagaggtgc ttgaaaagtt attgcatgat tatttacaac agtatgatac ctgtaacaat
    36301 aatatacact atgattatga gttgcctatt gactatatat catttgattt tttggatgaa
    36361 ttaaaactgt ttgaaccgta tggtgcagga cataagcctt ttacttttat ttcgcgcaat
    36421 tgctctattg caagctatta tattattaat gctaaacata taaaatgcat tgtagatgga
    36481 acaataaaac ttgaggcaat ggcatggaat ggcaaagata aatatgaagc tattgtagca
    36541 caaaagaaac cagtggatat aatctacacg gtaaagacta atgaatttaa cgggctcata
    36601 tcccctttat tgataattat tgatatgcat ctttccaatt aagatttaaa ataaaattcg
    36661 gtccttctat tcttttgcct gccttccttt gtttcgttgg atgctactgg ttttgtttct
    36721 ccgtaccctc tggagctgat tcgctcagga cttatgccat gttttataat atattctgca
    36781 actgcatctg ctcgtttttc tgatagcttc tggttatatg ttttgtcgcc ggtcgaatca
    36841 gtatgtccct gtacctctac tattaggccg ggattttttt tcatcatctc aaccagtttg
    36901 tccagtatag ttactgaatc tttcctgatg taccatttat caaaatcaaa atgtatatta
    36961 tcaataacca ccgatttacc aggttgtatc tgatccagca ctacgtttag ctgcttatcg
    37021 cttttattaa caatgacctt ttttggtaca tggccatctg attcaaaaat aacttcaaca
    37081 ttgtctttat cggggacagt gatatcacct tttgcattaa cagcagttga ttgtttatac
    37141 tcaccgtctt cgtctttata gtgaattgtt gctttagctt tcagcgggct tagctggtca
    37201 tttgttattt caactggcag cgttcgcatg ggcaattgct tttgtgtttg ctcagacgtt
    37261 ttttttggtt gtgtttcagg ggtgatagtt ttttgtgata gttctttttc tgaataacga
    37321 ggtgctggca tagatttttg ttcatttttt ttattatcac tatcagttgc agtttttgca
    37381 ctatcaaatc ctttgcttcc catatatcct gcaccaattg catagagata atttcctgct
    37441 ggtaaaaata agataccgtc ggcagcaacc ggctgggata caccgcccgg gaaaggtgca
    37501 acaaattccc accatagctg tgatgtgctg atatacattg cctttacttt tgcaacagga
    37561 tttttatcgg ttacttcatt aaaggcaata taaataacat cacgtatagt aacaaagtaa
    37621 ggagaggatt gttgcccaac aaaaatagtt gaaacaactt caccattttc aggattaata
    37681 gctttaatac tgttgttgtc aaatgaaaat aaaatggtcc tgtcagtaaa acttaatggt
    37741 gaagtgatag ttctattata acttttttgc cacattattt cgccattttt gatattcagg
    37801 cagtaaatgg tgtcatttac gggaacataa acatttttat tgacaacagg aggtaaaatt
    37861 ttaattggca cagaaaacgt acgctgccat accggttttc ccgttctttg ctcaagacaa
    37921 aaaacagtaa aagcttttgt ttcataattc attgattgag taataatata ttggtcataa
    37981 tataccggaa aaccactata ggtagcaata tccttatttt cccatacgat gtgtccattt
    38041 gcaatgtctc tggatgcaaa tactttccgt gttccataat agattgtatc agcatcaatg
    38101 attgggtcag cataaattcc gtcaaccatg acctgagttg cgatgccggt taatccttgc
    38161 ggttttgttt catccaccct gagccctgtt cttgcccaga tgaccttccc atttgttttg
    38221 tcaaaagcat agattgctat tgggtcactc acaagtaccg tattgccgta aacaagcggg
    38281 tatttaattt taccagcaga tttagaatat gctttaaatt gtgcggatat ggcatgcaca
    38341 tcatttaacc atagaatggt gccagcttct tcatctatac agtataaaaa agcattttta
    38401 tctacaaaat atattctgcc atcgcttacc accggattaa aaaccttgtc tttagctaaa
    38461 aaaagccatt taatctggtt gccgggaaca attatctcat cattgtttcc agtaaaaaaa
    38521 agattcccct tatatattgg ccagtcacct gcaaaggcta tggtggcgat agttactata
    38581 aaacatgtat agatgataat tcgattcata gagcgtattt tcctccagag attaaagtaa
    38641 taaaatcatc atcagcccat aatgcagtta ctgcaacaac tatcgcttta gcaaaaagaa
    38701 ggggagcgca acattatgag cattgacctt taaaacaaca gtgcttgcat tgttctacaa
    38761 gcttgctgta tatttttagt ttttttacat gctatatacc gtatgagtta tagctatgct
    38821 ggaatattat tccgaaaatt tcaagaaatt tttgaaatca ccggtataga atataaatat
    38881 tctattttat tctttacaaa ttgtgcctaa caatactgtt atacaaccat cgtaacaatg
    38941 ttttcaaatt aatattaata ctactattta gtatatcagg gtatggctac atcaacacaa
    39001 catgataaaa gctttttcaa cacacttatg gtggcaaccg gtattttcct tagcaggata
    39061 gcaggactta ttcgtgatag agtttttgca cattttttag gtaactcgga tgctgctgat
    39121 gcattcagag cagccttccg gattcctaat tttttgcaaa atctttttgg tgagggtgtt
    39181 ctttctgctt cgtttatacc agtatatgca aagttgattg cacaaa
//
LOCUS       DAOSOX010000003        39196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1073_length_39196_cov_8.675813, whole genome shotgun sequence.
ACCESSION   DAOSOX010000003 DAOSOX010000000
VERSION     DAOSOX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 39196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39196
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_39196_cov_8.675813"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLX22_00410"
     CDS             complement(<1..752)
                     /locus_tag="PLX22_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT18396.1"
                     /translation="MENFDFNEKPVAKEKNVFLLHLDIPRIILICAVIIGIVVTTFLL
                     GMNFSENKVVSNTALVSPDIPNDTLAQLDKGIDLGTSPQNPDFSTLDNAPDSLTDTLP
                     RVEEPKTYTENVTNKEANPGFSLANNDILSTQIIEQKEMKKTTPVKTTSKKNTATTKK
                     TVKKKQIVEVAQTEEIPSKNKAHRSWSIQVASYNTIFKAQKEAKYLHSMNYDAFVDKA
                     DISGKTYYRVKIGPILKEDKAIGMLQEIQSID"
     gene            complement(719..1309)
                     /gene="coaE"
                     /locus_tag="PLX22_00415"
     CDS             complement(719..1309)
                     /gene="coaE"
                     /locus_tag="PLX22_00415"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT18397.1"
                     /translation="MRIGVTGIYASGKGTVCSMFEQLGATVIDTDIIAREIVTPPSPV
                     LDSLVATFGRDILNPDGTLNRRYVANMVFNDANKVNALNAITHPAILCEVLQRTSNHS
                     TIYMVNTPLLFETEFYTYMDKTIVVIANQQQVVKRGQMRDGITEREINERLQHQISLN
                     EKMKLADYIIDNSKSLENTKKQVEELWKILISMKNQ"
     gene            complement(1314..>1976)
                     /locus_tag="PLX22_00420"
     CDS             complement(1314..>1976)
                     /locus_tag="PLX22_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18398.1"
                     /translation="PAVFSSPVIVSLNSHPLCQLIEQVFPATIGSIDSRTGTVLGDAV
                     SFAQFSGFSPIGIAGADFSFPHFITYVRSTEYHYRYAAAHTRLSPIETSNMQYIFQAS
                     KGTLVEQRYSRKVFIQYRNTIDALIYNTITYQINVSGLQLKNAVPLSTEDFFTLPAIM
                     HYHKKLSPQALKQPMDIHWERLYSYLYNDSIFNELMKASKVGDSYASQLKLLLKLLLQ
                     KR"
     assembly_gap    1977..2076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2077..2567)
                     /locus_tag="PLX22_00425"
     CDS             complement(<2077..2567)
                     /locus_tag="PLX22_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18399.1"
                     /translation="MYNIEYFLEQAKENFHTLRVIGEDGNTKYIHSKYYPTREASYLK
                     DIVNPEKYDVLIILGIGLGYHLAYLTEIIHSFTHIICIDINNYLTNACIPEHIHLLLQ
                     NPAIETIIIDPLMPEQLYKLLSINCSKGIQVIDHQASFAAFPSAFASIKNIVQQIIQK
                     SLS"
     gene            2648..3469
                     /locus_tag="PLX22_00430"
     CDS             2648..3469
                     /locus_tag="PLX22_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT18400.1"
                     /translation="MIIDSHAHIFTDDMIVHKDRYCDDKQFALLYYGKAKIANDALLI
                     DSMQNNGVDASVALGFTWQRNDYAQRHNEYMAMVQERHKGKVYAFGAIALDTDMYQQI
                     KNIKQMGLYGIGEVAFYVNGFTEEEETIVSNILAIAGDMELPVCLHVNEPVGHIYTGK
                     YTTDMQRLYALLSQYTNTTVICSHWGGGLLFYELMPEVKKSLQHVYYDSAASPFLYSD
                     TIYSIAITICGKEKILFGSDFPLLDYSRYNNSIQLLGENAQWIADQNVKKLLRLL"
     gene            complement(3470..3736)
                     /locus_tag="PLX22_00435"
     CDS             complement(3470..3736)
                     /locus_tag="PLX22_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18401.1"
                     /translation="MIKHCVIALVILSLLYPVACKKSTITPKLFVTIQNEMLESDMQP
                     ATKESIVAKYNLSLKEYEEYEKKVESDPQLKADVGKERLNIMKQ"
     gene            3862..5160
                     /gene="hisD"
                     /locus_tag="PLX22_00440"
     CDS             3862..5160
                     /gene="hisD"
                     /locus_tag="PLX22_00440"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT18402.1"
                     /translation="MLQIERLSELTKDDLYKLFQRFGADFTSIMVNTVIPIVQRVREE
                     GERAVLAFTKQFDGVALSSVYATIEEIAQSYEKTDTNVIDSFKKAIDNIKEFHLHQKR
                     DSFLYTRDDGITLGMLYQPIDSAAIYVPGGKASYPSSVLMGVIPAQIAGCKNIEIITP
                     PANDGSIPAYIGAMAYLLGITTIIKSGGAQGIALAAYGSGAIPKADIVVGPGNIYVTA
                     AKAYLFSQGIIQIDSLAGPSEVLVIADDSAQPKWVAWDLLSQAEHEEMAIAILVTTSE
                     RLAKQVIEYLDEDLSSGRGRSEIKRKAMQHARILLVSSVEEAIDFSNNYAPEHMEFIV
                     KNPMDYLTQIKNVGSLFLGAYAPVAVGDYYSGTNHVLPTGGAARFSSGLGVETFMHRT
                     SFQYLTPDGLRDAVPHVCTMSLVEGFDDKHGDSLTIRFKG"
     gene            complement(5150..5794)
                     /locus_tag="PLX22_00445"
     CDS             complement(5150..5794)
                     /locus_tag="PLX22_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT18403.1"
                     /translation="MNYHDNLYELLEVTPSSSKNEIRKAFCSKVKMLHPDTTGSSSER
                     FTLELQKIIEAYRILMDDTQRARYDATFFKQAAKPLKYYIPQKRVRYSISLKDLLKNQ
                     FLPKKMRRKDIIHKFDHDIEIVCTQQELHKGAIALVQLPSRILCPLCNGQHRDCYLCN
                     GLGRIASPSTFEIELPGDLTKTTIITVDLSNYRPDTFTNLTVKFLRIKITIISP"
     gene            5976..6692
                     /locus_tag="PLX22_00450"
     CDS             5976..6692
                     /locus_tag="PLX22_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18404.1"
                     /translation="MNKQVSLCQPGNGKGCCVCCGLFNLRNVSHNVLSQFLTEASIEV
                     SYGKIINNLQLRKYGSIKKIYDFRDSTTYVCPFMGFIFNNTPGCLVHPSINDGIDSRD
                     HSLFGKELCNDYFCPAYRILSDREKKILIRCTCDWYSYSIGIMDPLSFRWIIQQVEGI
                     LDIQIVPEIEQENAIVRCVNEALSMLGMVLNTLDAPLFYYSEPEYYLHMRIVSLNSES
                     MLNKNVREAIQRLIINNTRY"
     gene            complement(6686..7756)
                     /locus_tag="PLX22_00455"
     CDS             complement(6686..7756)
                     /locus_tag="PLX22_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOT18405.1"
                     /translation="MDVIPVILAGGAGTRLWPLSNEQKPKQFHNITGEGTLLELTINR
                     LKPLHPDFCLIVTARMYEDMSYEELHKTGLKGSIISEPMPRNTAPAILYAATYLSKLY
                     DDAIMLILPADHYIKKTDLFIQVLQKAIQQANNNYLVTIGITPTYPETGYGYIKAYDD
                     PSEVKPVEMFVEKPNLETAKKYLREGNYFWNSGIFVWKISVILDYYQKLMPQLYNAFQ
                     PLRDLTVEQLTQNDDLPWEIKRTVFSEIESISIDYGIMEKADYRYVIPGEFGWADLGS
                     WASIDDILNPDSNMNRSSSSKQVIFHLSKNCTVFPDKKLIAVVGLNDAVVVDSGDEIL
                     VMDKNASQDLREVVNHIKSKPQ"
     gene            complement(7972..8955)
                     /locus_tag="PLX22_00460"
     CDS             complement(7972..8955)
                     /locus_tag="PLX22_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOT18406.1"
                     /translation="MKKTTIISLLSIVCLTLLITCTSFDPAMFQKKPTIQFHSFQFKE
                     ITLNDMTTLFSIEIENPYPVTLYVDSIQSQIDIDSHTLLSLQSKDSLKLSKHSKAIKT
                     FAVTITYQQLLQAVNNYINRDSIMLSIKGTIGFSLPPSMQIVKPINVPFTIKQEIPTI
                     KPSIDIAHFKIIPPTLNELATMVKNRTLTISMLELNQVFADIQAFSQGKSIPDSILQL
                     DIPLSMSFDIIIKNKTKALLTGKSIDYNFILYDTEIAKGKPTIQSVNGTSTINCLTVL
                     STKNLSKGIVTALPKKNIHYMMKGSMLLDASFKDSTMPIPFSINHDGTISW"
     gene            9074..9913
                     /locus_tag="PLX22_00465"
     CDS             9074..9913
                     /locus_tag="PLX22_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18407.1"
                     /translation="MRRRIHFFMCSFLVICATLCGYHDDGFSQRTKSSQQAGEQRKAE
                     KGLKDNERFFYYINFSVSNSVVEQERLVCRKAILYDMFARFLYMKFRFNDAYKQIRKS
                     QELLIGLYLMVLSREIEEGKKLLDSVSPGPIMKNDYVSKHYCNLGYTNNEQAKLYMVM
                     ADNYRTSLYSMRLYKYIQALKKAKQAKRYAIIAYVTFQDKEFKKKFTIKDKGNNEPYI
                     YSYLKTKLEAIQDENEKKYLIPILGDSYYQLNNDYSYYDDVWSNGSLHELPEYAEYEK
                     EEE"
     gene            9916..12225
                     /locus_tag="PLX22_00470"
     CDS             9916..12225
                     /locus_tag="PLX22_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOT18408.1"
                     /translation="MNSRCIALFRIIGFVFIFSFISCTNVVLPQGLSQLGFYGDSVSI
                     IQTLEKKQKLSYTEHFMAGIAYKKNGDYKKAMYHFANSCFAQRQALTLTIYPQPVYSF
                     VSRLSTRSPLYNDAVYEIAKGFMYYQEYNYVLKFIDLIKKEKTGVYRDAQMLKSDALI
                     QLKKFEQAKETIEKTISQYHDVDSLVGLYLKLASLYEKQNSYLYAVQSYIKLLTISTK
                     SWRSQIAAKRILQIKEKNPDIVLLPDDIIMVVEALYNTKNYSDIVSLDSTWDKFFDNA
                     DIRYEFDSIRIKALCQLGKTGEIKQIYSQYKNSDFWDDCAVVIVRALWNSKKGEAISW
                     VNKLAETTDDGLKKTALYYKALYSIYRKEKNSQPVDEFVANYPNDALSQELLWLYAKN
                     NITSNKVAQYYLEKYYSMFGVKGKYADEVYFWLYILNSKENPEKAQTILYDLIAYRPD
                     SAYSWILLERRSPDYTVDHLSLDFSMAIQTLNFSTTLFTHAMLTYKERDEAKRLKRAA
                     LIKKSFSKVSELQYDKIPKLNEKLSLQDISKNNFVRIEHYFSIGYLEGINREFSVFAS
                     NSNNDVYIVSYLLGLRYNNYFMSAQALVQLLRRMNLTENIFLMSPDAINSLLPVPFDT
                     CINSAAKKYAVQEPFICAVIKAESLFNPYAESSAGAVGLMQLMLPTAKDIARKTGYGT
                     ITKDVLREPCISIEMGTYYIAWLMKNLNNNITLVAAAYNAGIGNVLKWKHDSEDLFTI
                     SVPFAETKGYIERIKKFYYQYMLLYDIRK"
     gene            12305..12997
                     /locus_tag="PLX22_00475"
     CDS             12305..12997
                     /locus_tag="PLX22_00475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOT18409.1"
                     /translation="MQRLIITVMAILLLMSINSFAKPVGKILELSGDIDITSMITGKR
                     IIPEEGTVIDGNDKIRTGRKSFVSIVLNDGSKLFIREISVLYVQDIKLKLTDQPTRIQ
                     IITGKLRVIAAKTMKGNSLVVYTPTAIAGVRGTDFGIIATQLETRVVIFQGNVEVANK
                     DKNILKSYILKDREETKVTYQQPPEAPVVVPDYILSKWFDTYSIDEKKNIIIKRKQED
                     GLLDRLLRKKDF"
     gene            12966..13751
                     /locus_tag="PLX22_00480"
     CDS             12966..13751
                     /locus_tag="PLX22_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT18410.1"
                     /translation="MIGFYEKRIFSYCISLCLIILCCSSVKPLTRSDTILSYYEEGYN
                     SNNWKTRVEAIKAVSSIKNERAENLIIKALNDSHNAVRIEALQILIQRPIKRARPIIR
                     DIALQDSNDNVRWSALMALSQFRDPRDASVFISNCANGDWLIREAAIVGLLSIDDFST
                     KYVNVDVIVRALHDSSISVIMATLRNLNIRHTQIYYTLIGMLKEKNNIMPSLTIAILN
                     ALQLYRLEENDKEVVINFLTHPNSQVRLAAFHTLKRQNQMNNM"
     gene            complement(13752..14411)
                     /locus_tag="PLX22_00485"
     CDS             complement(13752..14411)
                     /locus_tag="PLX22_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18411.1"
                     /translation="MKYFNFISSTMVLCLCLAIFTSPHAQENTTKQFNPLYDAEAVIQ
                     RLSYDNFKNIKLLRTAIYNFGGGEQEFNSLVDTYAEASALYFRNEMVASANLFTRNEK
                     DIKEVALRLAKIYKDQSEKLHINVIKMGVRHSLKASIEQAKPNPSVEPLISNASYAIK
                     SANDYLIRSKPVDAIYYFRRAKENCFKVYQILGEQLPEEYKKDVVDNQNKIYIAKEKK
                     N"
     gene            14702..15802
                     /locus_tag="PLX22_00490"
     CDS             14702..15802
                     /locus_tag="PLX22_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT18412.1"
                     /translation="MIRGAYIYSGKHYDYTMKICGLYILERLVYTLHKAGIQHIVLHL
                     DSDEKAFFNKTILHYITKKNIIVEECPNNKKPEKGYLVLPSNLFIQPHYFDNFKTYFK
                     ESNKMYEPLLREDQFLINSINDVKKAESLIVKYIIENTGGYIAKHINKRISIPISLQL
                     AKTRIHPNILTVINMMVGIVSAYCITQATYWSMVIGGLLFQLASIFDGVDGEVAKFTF
                     TVSKIGGWLDTIGDNGTLLLFLIAVSWLYFKNTSIGVACLVVSLCFIGLFWFIGMMVW
                     YLRKYSESGSLVAYDKEFLQKLPRDDVFVKVALSMKYITKKEFFALFFFCFSFTGKIF
                     LLIPIITFVVCAGACVLTIINIKYLKAVNTIR"
     gene            16149..16910
                     /gene="surE"
                     /locus_tag="PLX22_00495"
     CDS             16149..16910
                     /gene="surE"
                     /locus_tag="PLX22_00495"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT18413.1"
                     /translation="MNILITNDDGINAQGLQVLKGVLSDHYSVFSIAPGTERSGCSNA
                     IHMRTEITVEQIDEYSFAVDGFTADCVNIGLHSGLIPPVDLVISGINHGPNMGDDIYF
                     SGTVAGARTAYIFGVSGIAVSIDSYQESDYFKDASYFILELLKDYPIHVKKYPWFLNI
                     NYPDLPCHAIKGIQYTQLGKRYYRDAYRIVKQSGKFFTLELDGTIENEEKAGSDVTEL
                     RNGYIVITPLSIDCTDYNYMNRLQKRATTNHIWKS"
     gene            16898..17590
                     /locus_tag="PLX22_00500"
     CDS             16898..17590
                     /locus_tag="PLX22_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18414.1"
                     /translation="MEELALHKQIQQLLQEAHLLLSEKKNIRGAIATLEKLEKMDPEN
                     PLVLYNLAIAHAHCEDYARTAEYAVRCIALPMTFIDVKQVKKVYIYALIQSEDYEKAF
                     HEIETYDDIDEVLLQFEAFALEKMGDYNHALMIYERIYKKNSNNINVCNAIAYILANL
                     PEGNLDRALELAKKAYQREPHNAAYCDTIGYVYFKRGQLNMAKKYLKKAFAIKPDHRE
                     IRKHIAELLNIP"
     gene            17962..18570
                     /locus_tag="PLX22_00505"
     CDS             17962..18570
                     /locus_tag="PLX22_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18415.1"
                     /translation="MPAKGQKRRQQIVDTAKKMFIEQGFQSTHIGQVCEALNIARGTV
                     YQYFGNKREILYAILESIEESIDDILDPDDLRDLLKDNPSQKDIIKFNTERITECVKA
                     IIEEPIVIKLVFKDIVGIDEEVIERVDQFLKHLINIVSRDVEEIKKKGLYKKTLHSDI
                     TAIILIGSIMLLVYQFDKEKKDVTDKEVINALVSHYFTGVLK"
     gene            18682..19965
                     /locus_tag="PLX22_00510"
     CDS             18682..19965
                     /locus_tag="PLX22_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT18416.1"
                     /translation="MKKMSVIIIVLIACITNTFADVDCSKILNEQIYDEQSYANLSDE
                     CIENFKYIIVKGKDTEQKLKALKILRKSKVGKNCDFFKDILLSVNSMTVQQMIIEIFS
                     DIHDKQVLPIILPYLESPFYAVREAVIMALKKNGDDRMYPHIIKLAKSDDVINRIYAL
                     EALYHLYDNRFYEIVVQLLKDQNKSVRYFALECVKHNEIIATLPYIRAMAINDDNDEI
                     KVEAILVLASLHDKGSYYIFVRSLGSYSDGVREAAVDALIQFPSRDSALYLSRQLLAE
                     DETYIKRKIVKALITFRTAGDIRGLQKILENDENPSLRVYAAYALGVIKDDRTITMLI
                     RATGDDDYRVRAEAANALGNFKGKIVVISLLEKYEKESSTYVKTAILYSLKKICDKGS
                     LQKLFTLYCNENDVIHKMILQQTIHHIIARSFNQY"
     gene            20009..21655
                     /locus_tag="PLX22_00515"
     CDS             20009..21655
                     /locus_tag="PLX22_00515"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOT18417.1"
                     /translation="MKIAIAQIDPVIADISGNYQIISKYVHQAIQHGAELIIFPEMAT
                     IGYPPMDLLEHKKLIDDNLKSIETIATLSKDCGIICGYVDYDHDNPPMLLNAAACMYN
                     GNIQSKHYKTLLPTYDVFDELRYFSPASIQEPMKFKGVNIGLTICEDIWNDMSISEDS
                     FMEYRRYHVDPVENLIKKGAEFIVNISASPYVKGKNSIKWNKIQHVAKKYRVPIVYVN
                     QVGCNDSLIFDGSSFVIDTQGNFIMKAKAFEEDCVIFDTAVAYQPIVCEEDFLSDCTN
                     ALVLGIKDYVYKSGFNKVVLGLSGGIDSALTVCLATRALGADNVIGVTMPSVFSSKGS
                     VDDSIALAKNLGITIHTIPINDCYDTYLDILKPYFNALPFDSTEENIQARIRGNILMG
                     FSNKYGMLVLTTGNKSELSMGYCTLYGDMAGGLAVLSDIPKTMVYALSYYINRDKEII
                     PQSILDKAPSAELRENQKDQDTLPPYEILDQIIERYVELKMSAEDIINDGFDSAIVYN
                     VLRTIDKNEYKRKQAPLGLKVTGKAFGVGRRIPIVQRFKH"
     gene            complement(21657..22310)
                     /locus_tag="PLX22_00520"
     CDS             complement(21657..22310)
                     /locus_tag="PLX22_00520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT18418.1"
                     /translation="MSEYIVFYDLDHTLIAASSGQLIVKDLYQRHMLSLLTLGKGMVL
                     STLYRMGFISTESLIQKWVAKLAGMQCSFLERYSETFFDQTVKKYIYPQALESLYIHK
                     AQGAYLVLLSASLDFICKPVMKYLHFDAMLCTQLEVNNDHYTGSIKGSYCYGKEKLNR
                     ALQLCHNHNFNIHDAYYYADSLADIPVLEAVGHPHCVNPDQKLLRIAQSQGWTIHRW"
     gene            22527..23474
                     /locus_tag="PLX22_00525"
     CDS             22527..23474
                     /locus_tag="PLX22_00525"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT18419.1"
                     /translation="MNGLQRKDLLDIQSLSTEEILLICNSAKYFKALFTRSIKTVPIL
                     RGKTVCLLFYEPSTRTRISFELAAKRLSADLINIAVQTSSVVKGESLIDTVHTLEAYK
                     ADFIVMRHAASLAPHFLSRNIKSSVINAGDGNHAHPTQALLDAYSLIEKRGDIKGLHI
                     GIVGDILHSRVARSNMEIFKRLGAKVSLIGPPTLVPDDFTMYAVDIYYDFDTILPQLD
                     VVYMLRIQRERQQGSFFPSIHEYHKRFALTNERLKRCKNNVIVMHPGPINRGIEIDDE
                     VADSPCSIINEQVTNGVAVRMAIFYLLAGGAPLDEKEVE"
     gene            23474..24751
                     /locus_tag="PLX22_00530"
     CDS             23474..24751
                     /locus_tag="PLX22_00530"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT18420.1"
                     /translation="MTLLIKNGRVIDPLSKLDRCCDILIDEGTIVTIDTNIKQQRNIQ
                     IVDAQDCIVLPGLVDMHVHFREPGREDIETIAGGSTVAAKSGYTSVCTMPNTNPVIDS
                     QALVRYIKKQAESGFINVHPIAAITKGSLGEELTEMGELIEGGAIAFSDDGKPVLNST
                     TMRRALEYSRMFNVPIITHAEDTTLSGGGIMNEGNNSIFLGLKGIPKEAEEIMIARDI
                     LLARLTKGRLHIAHVSSKGSLDIIAMAKQHSLPVTCETAPHYFSLTDDAIADHLAMAK
                     MNPPLRTEEDRKAIIEGLRTGLIDVIATDHAPHLSNEKMQEIVFAPFGIIGLETAVPL
                     IITILVKENGFSYLQAFEKVTINPCTILGIDRGYIKEGAVADITIVNPEKKVIVDNNF
                     IISRCKNTPFLDREFFGLVTHTICNGKIVYPFR"
     gene            complement(24744..25397)
                     /locus_tag="PLX22_00535"
     CDS             complement(24744..25397)
                     /locus_tag="PLX22_00535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT18421.1"
                     /translation="MIQLLFIAIACDTLLHMVKPVNTINKLKKGESVTIVALGDSLTY
                     GWMVDTGYVDYFYDFLKIQYPEATITIFNKGVPGDTADGGLHRVGYDVIPNNPDCVFI
                     QFALNDCVYGYSPDIFGNYIRAIINTIQQNIQCDIVIITSVWFGDSPEAQNAYTFYDM
                     LLTIANEYHLPIAMTHQYWEDAVKKGTPLHELVQFDGVHPTEEGYYIMSLALQELFT"
     gene            25803..26576
                     /locus_tag="PLX22_00540"
     CDS             25803..26576
                     /locus_tag="PLX22_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT18422.1"
                     /translation="MDITNKRVLLTGGSSGIGKAMIDELHGFNTSIVVGDLNPEAIQH
                     HTNTTAMYCDVSKQKDIDTLFNKAITIMGGVDLCIANAGFAYYEKIESVDYQHIQKIV
                     AVNYIAPIYCLEKMMEINKGKKFSVAFTASAMAKLPLPGYALYSATKAALDGFAHSFN
                     FEKDSSVHLLIVYPIATETKFFATAGNNIPVPFPTQTPEKVAKEVIKGLQKEKRYVFP
                     SKLFRGMMVVNRFLPFVLYGYAKVEHYKLKKWLSVSKGQ"
     gene            26580..27236
                     /locus_tag="PLX22_00545"
     CDS             26580..27236
                     /locus_tag="PLX22_00545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT18423.1"
                     /translation="MGIAGVIFDLDGTLLDTIEDLAHSTNTVLARYGFATHDIEEYKQ
                     FVGDGMDMLIRRAIGNSSVSNLNDMVVSLKDEYAKSWFVTTKPYAGIEQVLEELSHKK
                     IKISVLSNKAHEFTVTMVQYFFPTIHFSSIMGLQDGMPPKPNPCGALYVAQIMAVEPE
                     HVAFIGDSGTDIEVAHRSNMYSIAVSWGYRPISLLQQHYPEAIAHTPDDILTIIEKHR
                     "
     gene            complement(27251..28747)
                     /gene="guaB"
                     /locus_tag="PLX22_00550"
     CDS             complement(27251..28747)
                     /gene="guaB"
                     /locus_tag="PLX22_00550"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT18424.1"
                     /translation="MKPKDGYSAEELLNSTQGLSYNDFIILPGYIDFNPDDVDLETNF
                     TRNVTLKRPLVSSPMDTVTESKMAISLALLGGIGIIHYNNTIEEQAAHVRKVKRYENG
                     FITDPVVLSPEHTIAHIDEIRESHGFSGIPITVDGKLNSKLIGIVTNRDIDFETDRTK
                     KLKEVMTTNLVTAGVGITLAQANEILKQSKKGKLPIVDNEGRLVALMSRNDLVTNKEF
                     PFASKDEKKQLMVGAAISTKDESKERLQELIKAGVNVVIIDAAQGNSIYQVEMIKYIK
                     KKYPDLDVVAGNVVTVEQCENLIKAGADALRIGMGPGSICTTQVTMAVGRAQATAVYR
                     CSKFARDYNIPTIADGGIATIGHIAKALSLGASTAMMGSMFAGTEEAPGEYFYENGIR
                     LKRYRGMASLEAMQKGGAKRYFAEDSKIMVAQGVSGAVVDKGPMFEYVPYLVQGLKHA
                     FQDMGIKTIATLHDKLYKEELRFELRSLSAQIEGGVHTVYSYKEPKYL"
     gene            complement(29010..30359)
                     /gene="radA"
                     /locus_tag="PLX22_00555"
     CDS             complement(29010..30359)
                     /gene="radA"
                     /locus_tag="PLX22_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOT18425.1"
                     /translation="MAKKLKSIFVCNECGATFQKWLGRCPECLQWNTIVEELSETITQ
                     KNEIPFENSIHPLSEMHSRQIDRIATGIYEFDLVCGGGIVPGSILLLGGEPGIGKSTL
                     ALQIAQQLTTLYCSGEETMQQIYLRSCRLQIKPEKIHISSVTNIDNIELLIKTNQPQL
                     VIIDSIQTLYSSEISSPVGSVSQIRYTASKLTEIAKQTGTCIVLIGHITKDGSIAGPK
                     VLEHIVDTVLYFEGDFTRDYRILRSFKNRFGSINEIALFTMTSQGLIEVKDKNKLFVQ
                     SQKASFPGSVISATIEGTRTILFEVQSLVTATSFTNPRRMADGFDVNRLNLLVAVIEK
                     HVKLSLSSFDIFINVAGGFAVNDTSCDLAIAAAIISSLKNFAISHSTGIIGELSLSGQ
                     IRPVSHILRRITEFTNSGVTSIILSKNNINEVSLPDVATTLIPITSIVELTKLLQPQ"
     gene            complement(30362..31237)
                     /locus_tag="PLX22_00560"
     CDS             complement(30362..31237)
                     /locus_tag="PLX22_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT18426.1"
                     /translation="MRMSNEARVGFMVMISFTLFVVLVGILTKFNISQKGYRIHVYFG
                     FLNDLRVGAPVKIAGGIKIGYVDEIRQTGEKTDVVVWIDKGYRLSKAATFSIFTSGVI
                     GEKYINVVIPPLQEDIGFLKEGDIKYGIDPASFDQMLQTFQSFMQDKSGAQILAEIFQ
                     NSNKFVANLNKMVDENRYDVRQSVGTARTAITTFSQQLQQFMQQMNVISKNMAYISEK
                     NREDITITLQNLSELTTSINKIAYRLEKGRGTMGKFLYDEEIYTNIRDASISAKELFS
                     RLEKDPSLLLFKQKK"
     gene            31536..32789
                     /gene="purD"
                     /locus_tag="PLX22_00565"
     CDS             31536..32789
                     /gene="purD"
                     /locus_tag="PLX22_00565"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT18427.1"
                     /translation="MRYLVIGSGGREHAIAWRLLHDGSATEVYVAPGNGGIDDRYRVA
                     IAINDFESMYTFCKSKSIDVVVVGPEAPLVNGIVDFLHNKGIRVFGPTAKAAQLEGSK
                     LFAKYIMQKYNVPTAQFREFKDKNDLLHYIESLTEYPIVIKLDGLAAGKGVGVCNTKH
                     DAIQFINTMVNHDTRVFVEDFCAGEEASVLGISDGKTVVPFIAAQDHKRAFDNDEGPN
                     TGGMGAYAPAPIVDSRILGRVHREILQPVIQGMNKEGIPFAGILYAGIIIKDGMIKVL
                     EFNVRFGDPETQVLLPLLNGKLGDYINAAIDGTLSSTNLSFRNKHAVTVVLASGGYPG
                     SYSTGLPIHGLDQIHNDILIFHAGTKREGNTIVANGGRVLNVTAIADSLEEAYNKVYS
                     VIPLLSFEGMHYRKDIGFRALKKRA"
     gene            complement(32724..33254)
                     /locus_tag="PLX22_00570"
     CDS             complement(32724..33254)
                     /locus_tag="PLX22_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT18428.1"
                     /translation="MKSLYYYNELVKRIDAFTSDIIAVYGTLLQCKEGCSGCCILESV
                     FPVEMVAILHWYSNQSPSVREAITNTKDNEYCIFLHEHTCRIYPYRPIICRTHGCPIM
                     VDTTIDVCPCNNGISFAANHILNVEHCNSMLAGINLLFIKEVNIPLLQKERIDLRTFF
                     QSSESDILSIVHSLKR"
     gene            complement(33259..34995)
                     /locus_tag="PLX22_00575"
     CDS             complement(33259..34995)
                     /locus_tag="PLX22_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT18429.1"
                     /translation="MTIQPQSDILYVKPYTDAYAIINIRLGGRGYIYANPKGEGTGKP
                     LVAKATTHDPIEFKNTRYLPGTMHISPIDNKPVYIYKNQTSLCIPFTVKTNREGLYSF
                     TISIDALLCNDSSCTPITGTIAQKVNVKNDAADFPQSHIAPYKEPQQMTYLEQSHNNY
                     TFNAIDMGYSSKGILHAIIFGIIAGFILNFMPCVLPVISLKLFSFVSYAHNNPRQIKS
                     MGLLFTLGIITSFIILAALAAFAGYNWGELFQHKTFIIVMAAIIFSMALSFFGIYTFN
                     VPSLPSKLQSANPYIDSYFKGVLATILATPCSGPFLGAILTWTLTQHPIVIFMIFINI
                     GIGMSLPYLILAMYPSLVRFIPKPGEWMIIFEQCMGFLLVATSIYLITLLDITALTQA
                     LWFILLLSLGLWQWGIFGNIARPLIHRITSFIILLAIVIISGLMLFTTKPSVSRIPLK
                     QLDWDAIVHNNSSHVSMIIFTADWCPNCKLVEGTTLKSKKVIDFIKSNDIHTYKADIT
                     TKNELAEELMQSLGARSIPFVAIFPKGEYFYQPLILRDMYTSNDLLKALQKAMDISNS
                     QQTLQLMTIPHH"
     gene            35267..35437
                     /locus_tag="PLX22_00580"
     CDS             35267..35437
                     /locus_tag="PLX22_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18430.1"
                     /translation="MMAQQITVVEQSIEQLNNLLKGLAAVRTGLSEKLVEVNVKTQGR
                     IEGLGTIIDSYQ"
     gene            complement(35534..35683)
                     /locus_tag="PLX22_00585"
     CDS             complement(35534..35683)
                     /locus_tag="PLX22_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18431.1"
                     /translation="MEGKITEHETAFLHQGIIGKQIKTHGGILSSGMVLFVRSSISSI
                     ILLEH"
     gene            36044..36676
                     /locus_tag="PLX22_00590"
     CDS             36044..36676
                     /locus_tag="PLX22_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18432.1"
                     /translation="MEKKLLIIAAGLLLLCQTIFAANKEINGTLGTSYADNPSKYGLD
                     ISLHYNWLLDPYFLTGLESGFFWVQWDRKIGVKQEGSVYVDVKADSNAYTIPIMFNAQ
                     VRLPNLQSKLYVVPFATVGFGYSFMILHYSRPDFTDSRTGKTYDSDSITKLFKGLSWE
                     LLFGVSLKPATDSKVDFYAECGYRKLPLKSGDLELDMSGFLLRFGVRYPL"
     gene            36774..37718
                     /locus_tag="PLX22_00595"
     CDS             36774..37718
                     /locus_tag="PLX22_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT18433.1"
                     /translation="MVPTAYLFDPVYMDHDTGWGHPERPERLVAIDQKLRQQPYYKSL
                     IKVEPKIGAKRYIELVHSPDYVKRVKEEIESGVHYLDSMDTAVCKRSYEVALLAVGGS
                     LNMCDAIISGEAIRGFCAARPPGHHAEYDYAAGFCIFNNIAIAAKYLQVEHGINKIAI
                     VDWDVHHGNGTQHTFERDRTVLYISTHQYPHFPGTGARSERGKGEGEGYTLNFPMPAG
                     SGDPEYMDVFENHIVPALMKFEPEIVLLSAGFDAHNADPLSYIGLSTEAYYKFTVLIK
                     QAANEYSGGRMIAFLEGGYDLEALANSVDMMMRAFIED"
     gene            38142..>39196
                     /locus_tag="PLX22_00600"
     CDS             38142..>39196
                     /locus_tag="PLX22_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT18434.1"
                     /translation="MYPLLFTPITINTLQIKNRIAYPALGLLYSYDSLLNDRYYEYFR
                     EKAKGGAGLVTVGPVGIDIAGSGRMALSLADDDKIPSFKKLTTIIKSEGAQTFVQLFH
                     AGAYSYSKLTDDVQPIAPSSVYSKYSKQVPREMTLEDIEMVQTAFVKAALRAQEAGFD
                     GIEIIASAGYLITQFLSPITNKRTDRYGGSFENRVRFPREVIEKVRKAVGFDFPLGIR
                     MAGNDFVKGSNTDRETPLFAKVYEDAGVDIINVTGGWHEAKIPQLPMELPRMGYAYLA
                     ATIKKAVHVPVMASNRIATPEAAEKILRDSMADMVNLGRVLIADPDWPTKAKEGKTNL
                     IRPCVGCNQGCTDTLFSG"
BASE COUNT        12518 a         6304 c         7819 g        12455 t
ORIGIN      
        1 ttatcgattg attgaatttc ctgtaacata cctattgctt tatcttcttt tagaatcggt
       61 ccaattttta cgcgatagta tgttttaccc gaaatatctg ctttatcaac aaaggcatca
      121 tagttcattg agtgtaaata ttttgcttct ttttgagctt tgaatattgt gttatatgat
      181 gctacctgaa tagaccatga acggtgtgct ttgtttttac taggaatttc ttcagtttga
      241 gccacttcaa cgatttgctt tttctttacg gtcttttttg ttgtagcggt attttttttg
      301 gatgtggttt taacaggtgt ggttttcttc atttcttttt gttcaattat ctgagttgaa
      361 agaatatcat tgttggctaa cgaaaagcct gggttagctt ctttattagt gacgttttct
      421 gtgtatgttt ttggttcttc aaccctgggc agagtatcgg tgaggctgtc aggagcatta
      481 tcaagtgttg aaaaatcggg attttgtggt gaagttccta aatcgattcc tttatcaagc
      541 tgtgcaagtg tatcattggg tatatcagga gataccagtg cagtgttaga aacgactttg
      601 ttttcgctaa agttcatacc taatagaaaa gtagtaacaa caataccaat gataacagca
      661 caaatcaaga ttattcgcgg gatatccaga tgcagaagga aaacattttt ttcctttgct
      721 actggttttt cattgaaatc aaaattttcc ataattcttc aacctgcttt ttagtatttt
      781 ctaatgattt ggaattatct atgatataat cggctaattt cattttttca ttaagtgaaa
      841 tttgatgctg caatcgctcg ttgatctctc tttcagtaat accatcacgc atctgtcccc
      901 gctttacaac ctgttgttga ttggctataa ccactatagt tttatccata taggtataaa
      961 attcagtttc aaaaaggagc ggggtattga ccatgtagat ggttgaatga ttggatgttc
     1021 tttgcagtac ttcacagagt attgctggat gagtaatagc attgagtgca ttgaccttgt
     1081 tcgcatcgtt aaataccata ttggctacat agcgtcgatt gagcgttcca tcagggttga
     1141 gaatgtccct gccaaatgta gcaactaaag aatccagaac agggcttggc ggggtaacaa
     1201 tttctcgtgc aatgatatca gtatcaataa ctgtcgctcc taactgttca aacatggagc
     1261 aaacagtgcc tttccctgaa gcgtagatac cagtaacccc aatccgcata gtattacctc
     1321 ttttgtagca aaagtttcaa taatagtttt aattgcgaag cataactatc gcctacctta
     1381 gaagctttca tcagctcatt aaaaatgcta tcattgtata aatatgaata tagccgctcc
     1441 caatgaatat ccatgggctg tttcaaagcc tgcggcgata gttttttatg atagtgcatg
     1501 attgctggca aggtaaagaa atcttctgta gatagtggta cagcattttt caattgaagc
     1561 cctgaaacat tgatctggta ggtaatggta ttgtagataa gtgcatcgat tgtgttgcga
     1621 tactggataa ataccttcct tgaatatcgc tgctcaacaa gcgtaccttt tgatgcctgg
     1681 aagatatact gcatattgct tgtttcaatt ggggataacc gggtatgagc tgctgcatag
     1741 cgatagtgat attctgtgga gcgtacatag gttatgaaat gagggaatga aaaatcagca
     1801 cccgctatgc caataggaga aaatccagaa aattgcgcaa aggatacagc gtcaccaagg
     1861 acagtgccag ttctactatc gattgaacca atggttgcag ggaaaacctg ttcaataagc
     1921 tgacacaatg gatgactgtt cagtgataca ataaccggcg atgaaaaaac ggcaggnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgg atagggattt ttgtatgatt
     2101 tgctggacta tatttttaat ggatgcaaaa gcagatggga atgcagcaaa cgacgcctgg
     2161 tggtcaataa cctgtatccc tttgctgcaa ttgattgaaa gcagtttgta tagctgctca
     2221 ggcatcaatg gatcaatgat gatggtttca attgcaggat tttgtagcag caaatgtata
     2281 tgttcaggta tacaggcatt tgtaaggtag ttgtttatat caatgcatat aatgtgggta
     2341 aagctatgga ttatctcagt taaatatgct aaatggtacc ccagcccaat acctaaaatt
     2401 attaaaacat catatttttc aggattgaca atatccttta aatatgatgc ttctctggta
     2461 ggataatatt tactgtgtat atacttagta ttgccatctt caccgataac acgcagtgta
     2521 tggaaatttt cttttgcttg ttccaaaaaa tattctatgt tgtacatagt cattgttcat
     2581 agcatggaaa tgcagttaat tttaaacaga tgacctgttt tatgtcaata cattatagga
     2641 tagcacaatg attatagata gtcatgctca tatctttact gatgatatga ttgtgcataa
     2701 agatcgctac tgtgatgata aacagtttgc gttgctctac tatgggaaag caaaaattgc
     2761 caatgatgcc ctattgattg atagcatgca aaacaatggt gttgatgcat cagttgcgct
     2821 gggatttaca tggcaaagaa atgactatgc acagcgccac aatgaatata tggctatggt
     2881 acaggaacga cataagggta aagtatatgc atttggggcg atagctctgg atactgatat
     2941 gtatcagcaa ataaaaaata ttaaacagat gggattatat ggtattggcg aggttgcttt
     3001 ttatgttaat ggatttactg aagaagagga aaccatagta agtaacatcc ttgcaatagc
     3061 aggggatatg gaattacctg tatgcctgca tgtcaatgaa cctgttggac atatctatac
     3121 cggtaagtat actaccgata tgcagcgctt atatgcactc ctgtcacagt atacaaacac
     3181 aacggtcatc tgttcacatt ggggtggagg gctactattt tatgaattga tgcctgaggt
     3241 aaaaaaatct ttgcagcatg tctactatga tagcgctgca tcaccctttc tttatagtga
     3301 tactatttat tcgatagcca ttacaatatg tggaaaagag aaaatacttt ttggtagcga
     3361 ttttcccctc cttgattatt cacgatacaa caatagcata caattgcttg gcgaaaatgc
     3421 acaatggatt gctgatcaaa acgtgaaaaa attgttgaga cttttatagc tattgtttca
     3481 taatattgag acgctctttc ccaacatcag cttttaattg gggatcgctt tcaacttttt
     3541 tttcatattc ttcatattct ttcagggaaa gattgtattt tgcaacgatt gattcttttg
     3601 ttgcaggttg catatcactt tcaagcattt cattttgaat ggtcacaaaa agttttggag
     3661 taatagttga ttttttacat gcaacaggat agagtaaact taatattaca agagcaatta
     3721 cacaatgttt tatcatagac cctccataaa tatttttacg gcaatgtttg tgtagtataa
     3781 taataattaa ttatatagtc aagacattat ctataataat gaatgtaaaa taacactaaa
     3841 caaagattaa aagaggtata tatgttacag attgagaggc tatcagagtt aacaaaggat
     3901 gatctatata aactttttca gcgctttggt gcagacttta catcaattat ggttaatact
     3961 gtaatcccca ttgtccagag ggtgcgtgaa gagggtgaga gggctgtgct tgcatttacc
     4021 aaacagtttg atggtgttgc attatcctct gtttatgcta caattgagga aattgcgcaa
     4081 tcatatgaaa agactgatac aaatgttatc gactcgttta aaaaggctat agacaatatt
     4141 aaagagtttc atctacatca gaaaagagat tcttttttat atacccgtga tgatggtatc
     4201 accctgggaa tgctttacca gcctatcgac agcgctgcca tttatgttcc cggtggtaaa
     4261 gcctcatatc catcttcagt cttaatggga gttattcctg cacagattgc agggtgtaaa
     4321 aatattgaaa tcataacacc accggctaac gacggatcaa ttcctgcata tattggtgcg
     4381 atggcatatc ttttagggat tacaaccatc atcaaatcag gtggggcgca ggggatagcc
     4441 ttagctgctt atggcagtgg agcaatacca aaagctgata tagttgttgg tccggggaat
     4501 atctatgtga cggcagcaaa ggcttatcta ttcagtcagg gtattattca gatagattcc
     4561 ctggcaggcc caagtgaggt gcttgttatt gctgatgata gtgctcagcc aaagtgggtg
     4621 gcatgggatt tactgtcgca ggctgagcat gaagaaatgg ccattgcaat tttggtaaca
     4681 acatcggaaa ggctggcaaa acaagtaata gaatacctcg atgaagattt gtcctccgga
     4741 cgaggaagga gtgagattaa gagaaaagca atgcagcatg cacggatatt gcttgtttca
     4801 tcggtagaag aagcaataga tttttccaat aactatgctc ctgaacatat ggaatttatt
     4861 gtaaagaatc ccatggacta tcttacacag ataaagaatg ttggttcatt atttttaggc
     4921 gcctatgctc ctgtggcagt gggcgattac tattcaggaa ccaatcatgt tcttccaaca
     4981 ggtggggcag ctcgcttttc ttcaggattg ggcgttgaaa cctttatgca ccgtacatca
     5041 tttcaatatc ttacaccaga tggattaagg gatgcagtac cccatgtgtg taccatgtcg
     5101 ctggttgaag ggtttgatga taaacatggt gattcactta ctatccgatt taagggctga
     5161 tgattgttat tttaatcctt aaaaatttta cggttaaatt ggtaaatgta tcagggcgat
     5221 agttggaaag atcgacagta atgatggtag tttttgttaa gtcgccgggc agctcaatct
     5281 caaatgttga ggggcttgca atacgtccca acccattgca gagatagcag tcacggtgtt
     5341 gcccattaca gagagggcat aaaatacgcg agggaagttg caccagagct atcgccccct
     5401 tatgcagttc ctgttgagtg caaactattt caatatcatg gtcaaatttg tggatgatgt
     5461 ctttgcgtcg catttttttt gggagaaact ggtttttcag aaggtctttc aatgatatgg
     5521 aatagcgtac ccttttttgc ggaatatagt atttcagtgg tttggcagcc tgtttgaaaa
     5581 aggtagcatc atagcgcgca cgctgtgtat catccatgag gattcggtac gcttcgatga
     5641 ttttctgaag ttcaagggta aagcgttcgc tgctagatcc tgttgtatca ggatggagca
     5701 tcttgacctt tgagcagaat gctttacgaa tctcattctt tgatgaagag ggtgttacct
     5761 ctaataattc atagagattg tcatgatagt tcataatgcc gatgtataag aatattttaa
     5821 aactgtatag tacagtatac tcatgaaatg cattcattgc aagttaaatt ataacggtta
     5881 tacctaatac gtgaattttt atgtatttgt gcacaccaga tagtgatata gtaataaaac
     5941 tgctgggtac aaattaatag agattatggt gtcgtatgaa taaacaggta tcgctttgtc
     6001 agcccgggaa tgggaaagga tgttgtgtgt gttgcggtct attcaatttg cgcaatgtat
     6061 cacacaatgt gctgtctcaa tttttaacag aagcttctat cgaagtcagt tatggaaaga
     6121 ttatcaataa tctgcaacta cgaaagtatg gcagcattaa aaagatatat gacttcagag
     6181 acagtacaac ctatgtatgc ccgtttatgg gttttatttt taataacacg ccgggatgtc
     6241 tggtgcatcc ttctataaat gatggcatcg atagcaggga tcatagcctt tttgggaaag
     6301 aactatgcaa tgactacttt tgccctgcgt atagaattct aagcgacagg gaaaagaaaa
     6361 tactcattcg ttgtacctgc gactggtata gctattccat tggtatcatg gatcctttaa
     6421 gcttcaggtg gatcattcaa caggttgaag gtattctgga catacagata gttcctgaga
     6481 tagaacaaga gaatgctatc gtgcgctgtg tcaatgaagc actatcaatg cttgggatgg
     6541 tattgaatac tctggatgct ccgctttttt attatagtga gcctgaatat tatttacaca
     6601 tgcgtatagt gtcgctgaat agcgaatcta tgcttaataa aaatgtgaga gaagcgatac
     6661 aacgtttgat tataaacaac actcgctact gaggcttgct tttgatgtga tttactacct
     6721 cgcgtaaatc ctgcgatgca tttttatcca ttaccagtat ttcatcacct gaatctacca
     6781 caacagcatc attgagacca acaaccgcta taagcttttt atcaggaaaa acggtacaat
     6841 tttttgaaag atggaatatt acctgctttg agctggatga acggttcata ttgctatcgg
     6901 gattcagtat atcatcaatg gaagcccagc taccaaggtc agcccagcca aattcgccgg
     6961 gtataacata gcgataatcg gctttttcca tgataccata atctattgat attgattcta
     7021 tctctgaaaa tacagtacgt ttaatctccc atggcaggtc atcattttgt gtaagctgtt
     7081 ccactgttag atcacgcaat ggctggaagg cattgtacaa ttgtggcata agtttttgat
     7141 aataatccag aataactgaa atcttccata caaagatacc actgttccag aaataatttc
     7201 cttctctgag atattttttt gctgtttcaa ggtttggctt ttccacaaac atttcaacag
     7261 gttttacttc tgatggatca tcatacgctt taatataccc atatccggtc tctggatagg
     7321 tgggagtgat gccgatagtt actaaataat tgttatttgc ctgctgaatg gctttttgta
     7381 gtacttgaat aaataaatcg gttttcttaa tataatggtc ggccggcagg ataagcataa
     7441 tagcgtcatc ataaagcttt gatagatagg ttgcagcgta aaggatagcg ggggcggtat
     7501 tccttggcat gggctctgat ataatgcttc cctttaaccc ggttttatgc aactcttcat
     7561 atgacatgtc ttcatacatc cgggcagtta caatgagaca aaaatcagga tgtaaaggtt
     7621 ttaacctatt gatggttaat tccaataacg tgccttcacc ggtgatgtta tggaactgct
     7681 tgggtttctg ctcattgctg agaggccaca accgtgtgcc tgcaccacca gccaatatga
     7741 caggtataac atccataaat aacctcgcct gcagtaatta cggtcaaccg tatgggcacc
     7801 tgtaaaaatc aggattatca ttattcacac tatgacaaaa ctttgttcaa ctacatgtac
     7861 ccacaaggga atgtcctgaa tctaattatt agaaattcct ctatggcata atgttattat
     7921 ttgttctata cgtcaattat taaaattgtc aataaccatt ataatgcact attaccagct
     7981 tatagttcca tcgtgattaa ttgaaaatgg tattggcata gttgaatctt tgaaactggc
     8041 atccaatagc attgacccct tcatcatgta gtgaatattt tttttaggaa gagcagtaac
     8101 aatgcctttg cttaaatttt ttgtactcag taccgttaaa caattgattg tagaggttcc
     8161 gttcactgat tgtattgtag gtttaccttt agctatctct gtatcataca atataaaatt
     8221 ataatcaata gatttgccgg taagcaatgc tttagtttta ttcttgatga taatgtcaaa
     8281 tgacatggat agaggaatat ccaattgcaa tatactatca ggtatgcttt tcccctgact
     8341 gaacgcctgt atatcagcaa atacctggtt gagttccagc atactgatag taagagtacg
     8401 gtttttcacc atggtggcaa gttcatttaa ggttggcggt ataatcttaa aatgtgcaat
     8461 atctattgat ggcttgatgg ttggtatttc ctgttttatt gtaaaaggga cattaatggg
     8521 ttttacgatc tgcattgatg gtggtaagga aaaaccaata gttcctttga ttgacagcat
     8581 tatggaatca cgattaatat aattgttaac agcctgcaag agttgttggt aggttatagt
     8641 tactgcaaat gtcttgattg ctttagaatg ttttgatagt ttcaatgaat ctttagattg
     8701 taatgataac aatgtatgtg aatcaatatc tatttgactt tgaattgaat ctacatacaa
     8761 tgtgacaggg taaggatttt caatttctat tgaaaaaaga gttgtcatat cattaagagt
     8821 aatttcttta aattggaaac tatgaaattg tatggtaggt tttttttgaa acattgcagg
     8881 gtcaaatgaa gtacaggtga taagtaatgt taaacaaaca atcgataata aggatatgat
     8941 ggtagttttt ttcattgttg ttttcctgat ttttactata attttatact attttgtatc
     9001 aaaaagagtg tacagtcaat cataaaatat gataaaaata aatgcccaaa agaaaatgaa
     9061 caaaggtata ataatgagac gaagaataca ttttttcatg tgttcgttcc ttgtgatctg
     9121 tgctacattg tgtggatatc atgacgatgg tttttcccag agaacgaaga gcagccagca
     9181 agcaggtgaa cagaggaaag cagaaaaggg tttaaaagat aatgaaagat ttttttatta
     9241 tattaatttt tcggtttcta acagtgtggt tgaacaggaa cgtttggttt gcaggaaagc
     9301 aatactctat gatatgtttg cccgcttttt gtatatgaaa tttcgattta atgatgcata
     9361 caaacaaata cgaaaatctc aggaattgct tattggatta tacttaatgg tactgtcgcg
     9421 cgaaatagaa gaaggaaaaa aattgctgga ttcagtatca cccggtccta taatgaaaaa
     9481 tgactatgtg tcaaaacatt actgtaattt agggtacact aataatgagc aggctaaact
     9541 atacatggtg atggctgata actatcgtac atcgttatat tcaatgcgac tgtataaata
     9601 tattcaagcc ttaaaaaagg caaaacaggc taaacggtat gctattatag cgtatgttac
     9661 attccaagat aaagagttta aaaaaaagtt tactataaaa gataaaggga ataatgaacc
     9721 gtacatctat tcgtatttaa agactaaatt ggaagccata caggatgaaa atgaaaaaaa
     9781 atatttaata cctattcttg gcgatagtta ttatcaatta aacaatgatt attcatatta
     9841 tgatgatgta tggagcaatg gttcattgca tgaattgcca gaatatgcag aatatgaaaa
     9901 agaggaagaa taattatgaa cagcaggtgc atcgcattat tccgtatcat cgggtttgtt
     9961 tttatatttt catttatctc gtgcactaat gttgtgttac cacagggttt atcacagttg
    10021 ggattttatg gggatagtgt aagtatcatc cagacattgg agaaaaaaca aaagctgtcg
    10081 tatacagagc attttatggc agggattgca tacaaaaaaa atggtgatta taaaaaagct
    10141 atgtatcact ttgctaatag ctgttttgct caaaggcagg ctttaacctt aactatctat
    10201 cctcaacccg tctatagctt tgttagcaga ttatcaactc gctcaccact gtataatgat
    10261 gcagtgtatg aaattgcaaa aggatttatg tattaccagg aatataacta cgtgcttaaa
    10321 tttattgatc ttataaaaaa agaaaaaact ggtgtatacc gggatgcgca gatgcttaaa
    10381 tccgatgcac tgatacaatt aaagaaattt gagcaggcta aagaaactat tgaaaaaacg
    10441 atatcacaat accatgatgt tgattcgctg gtggggctgt atcttaaatt agcgtcattg
    10501 tatgaaaaac agaatagcta tttgtatgca gtgcaaagtt atatcaagct gcttactata
    10561 agtactaaaa gctggaggtc tcagattgct gcaaaaagaa ttcttcagat taaagaaaaa
    10621 aatcctgaca ttgttctgtt gccggatgat ataattatgg ttgttgaagc attatataat
    10681 accaaaaatt atagtgatat agtatcactt gacagcacat gggataaatt ttttgataat
    10741 gccgatataa ggtatgaatt cgatagcata agaattaaag cattatgcca gttgggtaag
    10801 actggtgaaa taaagcagat atattctcag tataaaaata gtgatttttg ggatgattgt
    10861 gctgtagtta ttgtccgggc cctctggaat tctaaaaagg gtgaagccat atcatgggta
    10921 aataagttag ctgaaacaac ggatgatggc ttaaaaaaaa cagcccttta ttataaggct
    10981 ttgtattcaa tatatcgaaa agaaaaaaat tcacagccag tagatgaatt tgtagctaat
    11041 tatcctaatg atgctctgtc acaagaattg ttatggttat atgccaaaaa taatattacc
    11101 agcaataaag ttgcacagta ctatttagag aaatattact caatgtttgg tgttaagggg
    11161 aaatatgctg atgaagtcta tttctggtta tatattctaa acagcaaaga gaatcctgaa
    11221 aaagcccaga ctatccttta tgatttaata gcatatagac cagattctgc atatagctgg
    11281 atattgctgg aacggcgatc acctgattac actgttgacc atctgagtct tgatttttct
    11341 atggctattc aaacgctcaa tttttcaaca acgcttttca ctcatgcaat gttaacctat
    11401 aaagagagag atgaagcaaa acggttaaag agggctgctc ttataaaaaa atcatttagt
    11461 aaagtttcag aattgcaata tgataaaatt ccgaaattga atgaaaaatt atccttacag
    11521 gatatttcta aaaataattt tgtcaggatt gaacattatt tcagcatagg gtatctggag
    11581 ggcatcaaca gagaattttc agtatttgcc agtaacagca ataatgatgt atatattgta
    11641 tcctatttat tagggctgcg ctataacaat tattttatgt cagcgcaggc tttggtgcag
    11701 ttgctaaggc ggatgaatct gactgaaaat atttttttaa tgtcacctga tgctatcaat
    11761 agtttattac ctgtgccatt tgatacatgc ataaatagtg cagcaaaaaa atatgcagtt
    11821 caggagcctt tcatctgcgc tgttattaag gctgagtcat tatttaatcc gtatgcagaa
    11881 tcgtctgccg gggctgttgg tcttatgcag cttatgctcc ctaccgcgaa agatattgcc
    11941 aggaaaacag gatatggtac tataacaaaa gatgtattgc gagagccctg tatttccata
    12001 gagatgggga cgtattatat tgcatggtta atgaaaaatt taaataataa tataacattg
    12061 gttgcagccg catataatgc aggaattggt aatgttttga aatggaaaca cgatagtgaa
    12121 gatttattta ctatcagtgt accatttgct gagacgaagg gatatattga acgtataaaa
    12181 aaattttatt atcaatatat gctattgtat gatattagaa aataataatt attttccttt
    12241 tttgcataat tgatactata ttataaatta tagtcaaagt gtatattata actggaggtt
    12301 tgtgatgcaa agactaatta taactgtgat ggcaattctt ctgctaatga gtattaattc
    12361 ttttgcaaaa cccgttggga aaatactgga attatccggg gacatagata ttacctctat
    12421 gataacaggt aaacggatta tccccgaaga agggactgtc atagatggaa atgataaaat
    12481 aagaactggc agaaaatcat ttgtttctat tgtattaaat gatgggagta aactgtttat
    12541 acgagagatt tcggttctat atgtgcagga tattaaatta aagttaactg atcaaccaac
    12601 gaggattcag ataatcaccg ggaaattgcg ggttattgct gcaaaaacta tgaaaggtaa
    12661 cagcttagtt gtatatacac caactgcaat tgctggtgta cggggaactg attttgggat
    12721 tatagcaaca cagctggaaa cacgggtggt aatttttcag ggcaatgttg aggtagctaa
    12781 caaagataaa aacattttga aatcatacat tttaaaagat agagaggaaa ctaaggtaac
    12841 atatcaacag ccgccggaag ctcctgttgt tgttcctgat tatattttat caaaatggtt
    12901 tgatacatat agtattgatg aaaaaaagaa tatcatcata aagcggaagc aggaggatgg
    12961 tttacttgat cggcttttac gaaaaaagga tttttagtta ttgtatatca ttatgtttaa
    13021 taatactatg ctgtagttct gttaaaccac taacaagatc tgatactatt ttgtcgtact
    13081 atgaagaggg atataatagt aacaactgga aaacaagagt agaagcaata aaagctgttt
    13141 ccagtattaa aaatgaacgg gcagaaaatc ttattattaa agctctcaat gattcgcaca
    13201 atgctgtaag gattgaagca ttgcaaatcc ttattcaaag gccaatcaaa agggcacgcc
    13261 ctattatcag ggatattgca ttacaagaca gcaatgataa tgtgcggtgg agtgcattaa
    13321 tggcactttc tcaatttagg gacccccgtg atgcttcagt ttttatctct aattgtgcca
    13381 atggggactg gcttatcagg gaagctgcaa ttgtcggctt actcagtata gatgattttt
    13441 ctacaaaata cgttaatgtt gatgttattg tcagggcatt acatgattcg tctataagtg
    13501 ttattatggc aacattacgt aatctgaaca tacgacatac acagatctat tacaccctga
    13561 ttggcatgtt aaaagaaaaa aataatatta tgccatccct tactatagca atattaaatg
    13621 cattacagct ttataggcta gaagaaaatg ataaagaagt cgtcattaat tttttaacac
    13681 accccaactc gcaggtgcga ttagctgcat ttcataccct gaagagacaa aaccagatga
    13741 ataatatgta actaattctt cttttctttt gctatataaa ttttattttg attgtctaca
    13801 acatcttttt tatattcttc aggcaactgc tctcctaata tctggtatac tttgaaacag
    13861 ttttccttag ctctcctgaa gtaataaata gcatcaactg gttttgatcg tataagatag
    13921 tcatttgcac ttttaatagc ataacttgcg ttgctgatta atggctcaac agagggattt
    13981 ggttttgcct gttcgatgga tgccttcagc gaatgacgca ctcccatttt aattacatta
    14041 atatgtaatt tttcagattg atctttatat atttttgcca accgcaaggc tacctcttta
    14101 atgtcttttt cattcctggt aaagagatta gccgaagcaa ccatttcatt tctgaaatat
    14161 agtgcactgg cttctgcata ggtatcaaca agactattaa attcctgttc gccacctcca
    14221 aagttgtaaa tagccgttct taataatttt atatttttaa aattatcata acttaagcgc
    14281 tggattacag cctctgcatc atataatgga ttaaattgtt ttgtagtgtt ttcctgtgca
    14341 tgaggtgatg taaatatagc taaacataag cacaaaacca tggttgatga aataaaattg
    14401 aaatatttca tgttgctatc tcctttataa gacttatata ctttaaattt gtattatgta
    14461 tggaatcata taatagatta aatgttgaag tcaagtaaaa tgttatcgtt actatttaaa
    14521 aaacaggttg acgaaataga gagatttgtg cggtattaat gaaacattgc tattattcaa
    14581 ttttttattg gatttatagt gaggacaatg cgatagtatg ttgttgccat aagcatgtgt
    14641 tacatgctac atgttgttgt atagttttga tacatactaa aactacaagg atggcttctc
    14701 tatgatacgt ggtgcatata tatatagtgg taaacactat gattatacaa tgaagatctg
    14761 tggattatac attcttgagc gattagtgta tacgcttcat aaagcaggga tacagcatat
    14821 agttcttcat cttgatagtg acgaaaaggc attttttaat aaaacaatat tgcactatat
    14881 aacaaaaaaa aatattattg tggaagagtg cccaaacaat aaaaaaccag aaaaaggcta
    14941 tctggtattg ccatcaaatc tatttattca acctcattat tttgataatt ttaaaacata
    15001 ctttaaagaa agcaacaaga tgtatgaacc tctattgcga gaggatcaat ttttaattaa
    15061 ctcaatcaat gatgtaaaaa aagcagaatc attgattgta aagtatatta ttgaaaacac
    15121 tggtggatat atagcaaaac atattaataa acgtatctcc attcccatta gcctgcaatt
    15181 agcaaaaaca agaattcatc ccaatatatt gacagtcatc aatatgatgg ttggtattgt
    15241 aagtgcttat tgcataactc aggctaccta ctggagcatg gtaatagggg gattattatt
    15301 tcaactggca tccatttttg atggtgttga tggtgaggtt gcgaagttta cgttcacggt
    15361 atcaaagatt ggcggctggc ttgacacaat tggtgataac ggaacgctcc ttttgtttct
    15421 gattgctgtg tcatggttat attttaaaaa tacctctata ggggttgcct gccttgttgt
    15481 aagcctttgt tttattggtt tgttctggtt tattggcatg atggtatggt atctaaggaa
    15541 atatagtgaa tcaggttctc tggtagcgta tgataaagaa tttttgcaga aattgccacg
    15601 cgatgatgtg tttgtaaaag ttgccctttc catgaagtac attacaaaaa aagagttttt
    15661 tgcgctattc tttttttgtt tcagctttac cgggaagata tttttattaa tacctattat
    15721 aacttttgtt gtatgcgccg gagcctgtgt gctgacaatc atcaatataa agtatttaaa
    15781 agcagttaat acaatacggt agcccttggc gatagtatct ggtaatagag tgcttgcatg
    15841 ggaaggaata atgttattta caatgggaac agttttttgc tgagcatgct gaacaagatt
    15901 gtttatggac agaggcactt atcagtgata taattttttc agtattatgt gaatggcact
    15961 gaggacatgg tgaagggtta ctatcgccca tcttcctgag ttcgctgtag atatggttgc
    16021 aattgttaca tttaaattcg tagattggca tagacgcacc atcacaggag tattctataa
    16081 tacaattata gcacctattt attttttata aaaaatgtct atagtacagt aactatcacg
    16141 agtaacgtat gaatatttta attaccaatg atgatggtat taatgcgcag ggattacagg
    16201 tattgaaggg ggttttatca gatcattatt ctgttttttc cattgcgcct ggtacggagc
    16261 gtagtggctg ttccaatgcc atacacatgc gcacagagat tacagtagaa cagattgatg
    16321 aatattcatt tgctgttgat ggttttactg cggattgtgt caatattggt ttacacagcg
    16381 ggctgatccc accggttgat ctggtgatat ccggtattaa ccatggcccc aatatgggtg
    16441 atgatatata tttttcaggg acggttgctg gagcacgaac agcatatatt tttggtgtaa
    16501 gcggtattgc tgtgtctatt gacagctacc aggaatcaga ttattttaaa gatgcgtcat
    16561 attttatact ggaattattg aaggattatc caattcatgt aaaaaaatat ccatggtttt
    16621 taaatatcaa ttatcctgat ttgccgtgtc atgcaattaa agggattcag tatacccagt
    16681 taggaaaacg gtattacagg gatgcatacc gtattgtaaa acaatcggga aagtttttta
    16741 ccctggaatt ggatgggacc attgagaatg aagaaaaggc cgggagcgat gtaactgagt
    16801 tgcgcaatgg gtatattgtt attacaccat tatccattga ttgtaccgat tataattata
    16861 tgaatcgatt gcaaaaacga gcgacaacaa atcatatatg gaagagttag cattacacaa
    16921 gcagatacaa cagctattac aagaagcaca tctcttgctt agcgaaaaga aaaatattag
    16981 aggtgccatt gccactctgg aaaagcttga aaaaatggac cctgaaaatc cattagtttt
    17041 gtataatctg gctatagccc atgctcattg tgaggattat gccagaacag cagagtatgc
    17101 agtccgttgt attgcattgc ctatgacttt tattgatgtg aagcaggtta agaaggtata
    17161 tatttatgct ttaattcaat cagaagatta tgaaaaagct ttccatgaga tagaaacgta
    17221 tgatgacata gatgaagtgc tattgcagtt tgaggcattt gctttagaaa agatggggga
    17281 ttataaccat gcattgatga tctatgaaag aatttataaa aaaaattcta ataatataaa
    17341 tgtatgtaat gctatcgcat acatcctggc taacctgcca gaaggcaatc tggacagggc
    17401 attggagtta gcaaaaaaag cctatcaacg tgaaccccat aatgcggcat attgtgatac
    17461 aattgggtat gtatatttta aaagggggca actgaatatg gctaagaaat atttaaaaaa
    17521 agcttttgca ataaagcctg accacaggga aatacgcaaa catattgccg aactattaaa
    17581 tataccatag cataataatg ttgtgaaaat catatgctgg agatagttat aatatgttct
    17641 tattatgcca aagtgttgaa ttataacaat attaaattta taaaaagttg ttgacgtgaa
    17701 aaagaaggtt agatataaag tttaaattca ataatctttt tttatataat ttcatataaa
    17761 tgttaacgat aggtattata gcattgaata tttatggcga aagaggattg ttactctatg
    17821 agtaaagaat tgcaggaaaa agcagaaaag atgattaaag atcttcagga agcattacag
    17881 cctataaaag aagaactatt gcgtgtgaaa ggggaactta aatagaataa tatattacta
    17941 ttgaatgaaa gaggtattat aatgccagca aaaggacaaa aacgacgaca gcaaatagtt
    18001 gacactgcaa aaaaaatgtt tatagagcag ggatttcaga gcacacatat tggacaggtt
    18061 tgtgaagcat tgaatattgc acggggtact gtttatcaat attttggtaa taaaagagaa
    18121 attctctatg ctattttaga atcaatagaa gaaagtattg acgacattct tgatcctgat
    18181 gatttgcgcg atcttttaaa agataatcca tctcaaaaag atattataaa atttaatact
    18241 gagagaatta cagaatgtgt gaaagctatt atagaagaac ccatagtaat aaaattagtt
    18301 tttaaagata ttgttggtat tgatgaagaa gttattgagc gcgttgacca gtttttaaaa
    18361 catttaatca atatagtatc acgagatgtt gaagagataa aaaagaaagg tctttataaa
    18421 aagacattac attctgatat tacagctatt attctcattg ggtcaattat gctgctggta
    18481 tatcagtttg ataaagagaa aaaggatgtt acggataagg aagttatcaa tgcacttgtt
    18541 tctcattatt tcaccggtgt attaaaataa ttgttgcgac aatagtggta ttatatcccg
    18601 gcttatatga gccgggtttt ttgttgatat aaatcatata tatcaatatt gtgactcaaa
    18661 tatttgtatg gtgatagttt tatgaaaaaa atgagtgtga ttattatagt tcttattgct
    18721 tgtattacca atacttttgc cgatgttgat tgctcaaaga ttttgaatga acagatatat
    18781 gatgaacaat cgtatgcaaa cctttcagat gaatgtattg aaaattttaa atatattatc
    18841 gtgaaaggta aagatacaga acaaaagcta aaagcgttaa aaatactacg aaaaagtaaa
    18901 gtgggtaaaa actgtgattt ttttaaggat atattattat cagttaattc tatgactgtt
    18961 cagcaaatga taattgaaat tttttcagat attcatgata aacaggtctt gcccatcata
    19021 ctaccctatt tagaatcacc tttttatgca gtacgcgaag cagtcataat ggcattgaag
    19081 aagaatggtg atgacaggat gtatcctcat attattaaat tagcaaaaag cgatgatgtt
    19141 atcaatagaa tttatgcgct ggaagcatta taccacctgt atgacaatag attttatgaa
    19201 attgttgtgc aattattgaa agatcagaat aaatcagtgc gatattttgc acttgaatgc
    19261 gttaaacaca atgaaatcat tgcaacccta ccctatattc gtgctatggc tattaatgat
    19321 gataatgatg agataaaggt tgaagctatc ctggttttag cctcattaca tgataagggt
    19381 tcctattata tttttgttcg atcactgggt agctattcgg atggcgtaag ggaagcagca
    19441 gttgacgcat taatacaatt tccttcacga gatagtgctc tttatttatc acgacagtta
    19501 ttagctgaag atgaaaccta tattaagcga aaaatagtta aggctcttat aacgttcagg
    19561 actgctggag atattcgcgg tttgcaaaaa attctggaga acgatgaaaa tccatcgtta
    19621 cgtgtgtatg cagcctacgc tcttggtgtg attaaagacg atcgtacaat cacaatgctc
    19681 ataagagcaa cgggtgatga cgattacagg gtacgtgctg aggctgctaa cgcactggga
    19741 aatttcaaag gaaaaattgt tgtcatttct ttgctggaga agtatgagaa ggaaagcagt
    19801 acgtatgtta aaacagcaat actctattca ttaaaaaaaa tttgtgataa aggttcgtta
    19861 cagaaattat tcactctata ctgtaatgaa aatgatgtga ttcataaaat gattttacag
    19921 caaaccatac atcatataat agcacgaagt tttaatcaat attagcatgt aattttacaa
    19981 tactataatg gaagtaaaag gtagctttat gaagatagct atagcgcaaa ttgatccggt
    20041 cattgcagat atttcgggta attatcaaat aatcagcaaa tatgttcatc aggctataca
    20101 acatggtgct gaattgatta tttttcctga aatggctact attggctatc ccccgatgga
    20161 tttactggag cataaaaaac ttattgatga caaccttaaa tccatagaaa ctatcgcaac
    20221 attgagtaag gattgtggta ttatttgtgg gtacgtggat tatgatcatg ataatccccc
    20281 catgttattg aatgctgctg catgtatgta caacggcaac atccaatcaa agcattacaa
    20341 gacacttttg cctacgtatg atgtgtttga tgaattacgt tatttttcgc cggcatccat
    20401 tcaggaacca atgaaattca aaggggtgaa cataggttta acaatctgtg aagatatctg
    20461 gaatgatatg agtataagtg aagatagttt tatggaatat cgccgttatc atgttgaccc
    20521 tgttgaaaat ttgataaaaa aaggcgcaga gtttatagta aacatttctg catcacctta
    20581 tgtaaagggg aagaattcca tcaaatggaa caagatacag catgttgcaa aaaaataccg
    20641 tgtgccgata gtttatgtca atcaggttgg ttgtaatgat agccttattt ttgatgggag
    20701 tagttttgtc atcgatacac agggcaattt tataatgaag gcaaaagctt ttgaagagga
    20761 ttgcgtaatt tttgatacag cagttgcata tcaacccatt gtctgtgagg aagatttttt
    20821 atctgattgt accaatgctc tggtgctggg tataaaagat tatgtatata agtcgggttt
    20881 taacaaggtg gtgttgggtt taagtggtgg tattgattca gcgttaactg tctgtcttgc
    20941 cacgagagcc ttaggagctg acaatgttat aggggttacc atgccttctg ttttttcgtc
    21001 aaagggaagt gtggatgatt caatagcact ggcaaaaaat cttggtatta ccatacatac
    21061 cattcctatc aatgattgct atgataccta tcttgatata ctaaaaccat attttaatgc
    21121 attaccattt gatagcaccg aagaaaatat tcaagcccgc atccggggaa atatccttat
    21181 gggcttcagc aataaatatg gtatgttagt attgacaacg gggaacaaat cagagctgtc
    21241 aatgggatac tgtacattat atggcgatat ggcaggagga cttgctgtat tatctgatat
    21301 tcccaaaacc atggtatatg cactctccta ttatataaac agggataaag aaataatacc
    21361 tcaatcaata ctggataaag ctccttctgc tgaattgcga gaaaatcaaa aagatcagga
    21421 tacattacct ccttatgaaa tccttgatca aataattgaa cgatatgttg aattaaagat
    21481 gagtgctgaa gatattatta acgatggctt tgattcagca atagtgtata atgttctaag
    21541 aactatcgat aaaaatgaat acaagcgaaa acaagcaccc ctggggttaa aggtaacagg
    21601 taaagctttt ggtgttggga gacgcatccc aattgttcag cggtttaagc attaaatcac
    21661 catctatgga ttgtccagcc ctgtgattga gcaatgcgca ataatttttg atcgggatta
    21721 acacagtggg ggtgcccaac agcttccagt accggaatat ctgccaatga gtcagcatag
    21781 taataggcat catgtatgtt gaaattatgg ttatgacata gttgcaatgc tctgtttaat
    21841 ttttctttcc catagcaata cgatccttta atagaaccgg tgtaatgatc attgtttact
    21901 tctaattgag tgcaaagcat tgcatcaaaa tgtaaatatt tcatgacagg tttacagata
    21961 aaatctaagg atgcagatag caatacaaga tatgctccct gtgctttgtg tatgtataat
    22021 gattccagtg cctgcgggta gatatatttt tttactgttt gatcaaaaaa agtttctgaa
    22081 tagcgttcaa gaaaactgca ttgcatgcca gccagctttg caacccattt ctgtattaat
    22141 gattctgtgc tgataaaacc catgcggtaa agtgttgaaa gtaccatacc cttgccaagg
    22201 gtaagcaatg atagcatgtg acgctggtaa aggtctttta caattaattg ccctgaactt
    22261 gcagcaatca gtgtatgatc aaggtcgtaa aagactatgt attcactcat acaaacacca
    22321 tacaataaaa aaatttacag gatgaatata catggatgtt ataagaatag tacaatgaca
    22381 ccatatttta caaaaattat tgaaaatatg attgcaatta atagtaattt cataaaattg
    22441 atataaaaag aggatgtatg aatgcattaa tactgtgcaa gttgcagctt ttttcatatc
    22501 agtttatata aggtttatag tactctatga atgggttaca aagaaaagat ttattagata
    22561 ttcaatcgtt atcaactgaa gagatactgc tgatctgtaa ctcggctaaa tatttcaaag
    22621 ccctttttac acgttctatt aaaactgttc ccatattgag aggtaaaaca gtatgtttat
    22681 tgttttatga accatcgaca cggacaagaa taagttttga gttggctgca aaacgtcttt
    22741 cagccgacct tattaatatt gctgtacaga catccagtgt ggtaaaaggt gaatctttaa
    22801 tagatacggt acatacttta gaagcgtaca aagcagattt catagtaatg agacatgcgg
    22861 cgtcattagc gccgcatttt ttatctcgaa atataaaatc atcggtaatc aatgcaggag
    22921 acggaaatca cgctcatcca acacaggcat tgctggatgc ctattcactc attgaaaaaa
    22981 gaggagatat taaagggctt catataggga ttgttggcga tatactccat tcacgggtag
    23041 cgcgttcaaa tatggagata tttaagcggc tgggagcaaa ggtaagttta ataggcccgc
    23101 ctacattggt accggatgat tttaccatgt atgccgtgga tatctattac gactttgata
    23161 ctatcttacc acagcttgat gttgtttata tgctacgaat acagcgggaa cgccagcagg
    23221 gttcattctt tccttctatt catgaatacc ataaacgttt tgctttaacc aatgaaaggt
    23281 taaagcgatg taaaaacaat gttattgtga tgcatccggg tcccatcaac cgggggatag
    23341 agattgatga tgaggttgcc gatagtccct gctctataat caatgaacag gttaccaatg
    23401 gagtagcggt acggatggca atattttatt tattagctgg cggagcgcca cttgatgaaa
    23461 aagaggtgga ataatgacat tgcttattaa aaatggtaga gtcattgatc ccttatctaa
    23521 acttgacaga tgctgtgata tcctgattga tgaagggact attgtcacca tagataccaa
    23581 tataaaacaa caaagaaata tacagatagt tgatgcccag gattgtattg ttcttcctgg
    23641 tcttgttgac atgcatgtcc atttcagaga gccagggcgc gaagatattg aaacgattgc
    23701 aggcggatca acggtggctg caaaatcagg gtataccagt gtatgcacca tgcccaatac
    23761 aaatccggtc attgatagtc aggcacttgt gcgatatata aaaaaacagg ctgagtccgg
    23821 atttattaat gtgcatccca ttgctgcaat caccaaaggg tcattgggag aagaactaac
    23881 ggaaatgggg gaactcattg agggtggagc aattgcgttt agtgatgatg gcaaacccgt
    23941 tctaaattcc acaacgatgc gccgggcatt agaatattca aggatgttca acgttccaat
    24001 aattacccat gctgaggata caacattgtc cggtggcgga ataatgaatg aaggtaataa
    24061 ttcaatcttc cttggattga aaggaatccc taaagaagct gaagaaataa tgatagcaag
    24121 ggatatatta ctggcacggc ttacaaaagg aagattgcac attgcccatg tatcctcaaa
    24181 aggttcactg gatattattg caatggctaa acaacattcc ctgcctgtca cctgtgaaac
    24241 agcaccacat tatttttcgt taactgatga tgctatcgca gatcatctgg caatggcaaa
    24301 gatgaaccct cccttacgga ctgaagagga cagaaaggca atcattgaag gattgcgaac
    24361 agggcttatt gatgtcattg caactgatca tgcaccccac ttatcaaatg aaaagatgca
    24421 ggagatagta tttgcaccat ttggtatcat aggactggaa actgctgtac cattgattat
    24481 taccattctg gtaaaagaaa atggattcag ctatttacag gcatttgaaa aggtaaccat
    24541 caatccatgc acaattttag gtatcgatag aggctatatc aaagagggtg ctgtggcaga
    24601 cataacaata gtaaaccctg aaaagaaggt tattgttgat aacaatttta tcatttcacg
    24661 atgtaaaaat accccatttt tagacagaga attttttgga ttggttaccc atacaatctg
    24721 caatggaaag attgtttacc cattcaggta aatagctcct gtaatgctaa tgacatgata
    24781 tagtatccct cttcggttgg atgcactcca tcaaactgta ccagctcatg caatggtgtg
    24841 ccttttttta ctgcatcttc ccagtactga tgggtcatag caataggaag atgatactcg
    24901 tttgctatgg ttagcagcat atcataaaat gtatatgcat tctgtgcttc tggagaatca
    24961 ccaaaccaca ctgaggtaat gataacgata tcgcactgta tattttgttg tattgtattt
    25021 ataatagccc gtatatagtt gccaaaaata tccggggaat agccatacac gcaatcattt
    25081 aaggcaaact gtatgaacac acaatcagga ttattgggaa tcacatcata tccaaccctg
    25141 tgtaatccac catctgccgt atccccggga acacctttat tgaagattgt gatggtagcc
    25201 tcaggatatt gaatctttaa aaaatcataa aaataatcta catatcctgt atcaaccatc
    25261 catccatagg tcaatgaatc gcctaatgcc acaatagtga cagattcacc ttttttgagc
    25321 ttgttgatgg tgttaactgg ttttaccata tgcaacaatg tatcacaggc aatggctata
    25381 aacaataatt gaatcataaa aaatataaga aatggaaaat aataaacatg tgggcgatag
    25441 ttaccggtaa tctatcttct gaaaaaatga agtatacatt tttgatggat ttgtgagcac
    25501 aaagtattga tataaacatt ttttaaaaat gtttataata cagaaaaatg tggttgttaa
    25561 aagcaccaaa cagtaaatat gcttgaaaaa ttacagatgt taatcttgag tcttaagtac
    25621 tgtgtttatc atctttaggt gaatattcaa aagtatggga ataattatag catatccttg
    25681 tttaatatgc attgcaataa ggttcgttgc aaatcttagc aaatattttc attatacatg
    25741 caatgggagt attattataa agcaaagtta aggcaacata ctattaatca ttgaggtgta
    25801 atatggatat tactaataag cgtgtattac ttaccggtgg atcgtcaggt attgggaaag
    25861 caatgataga tgaattgcat gggtttaaca catcaatagt tgttggtgat cttaatcctg
    25921 aggctataca acaccataca aacactactg ccatgtactg tgatgtgagc aaacaaaaag
    25981 atattgatac cttgtttaat aaggcaatta ccatcatggg tggtgttgat ctgtgtatag
    26041 ctaatgcagg atttgcctat tatgaaaaaa tagaaagtgt agattaccag catatacaaa
    26101 agatagttgc tgtaaactat atagctccca tctattgttt agaaaaaatg atggaaataa
    26161 ataagggcaa aaaatttagt gttgcgttta ccgcttcagc aatggcaaaa ctccctttgc
    26221 cgggttatgc gctctattct gctacaaaag ctgctttaga tggatttgct cattcattca
    26281 attttgaaaa ggatagcagt gtccatcttt tgattgtgta tccaattgca acagagacaa
    26341 aattttttgc aacagctggt aacaatatac cagtgccatt cccaacacaa actcctgaaa
    26401 aagttgcaaa ggaggtaata aaaggtttac aaaaagaaaa acgatacgtt tttccttcaa
    26461 agcttttcag aggcatgatg gttgttaacc ggttcctgcc tttcgtgctg tatggctatg
    26521 caaaggttga gcactataaa ttgaaaaagt ggctttcagt ttcaaagggt caatgattga
    26581 tgggtattgc tggtgttatc tttgatctgg atggtaccct tcttgatacc attgaagacc
    26641 tggcacatag tactaataca gtgttagctc gttatggttt tgcgactcat gacatagaag
    26701 aatataagca atttgtaggc gatggcatgg atatgttgat tcgcagggct ataggcaata
    26761 gttcagtatc gaatcttaat gatatggttg tatccttaaa agatgagtat gctaaaagct
    26821 ggtttgtcac tacaaaacca tatgcaggca ttgaacaggt tttagaggaa ctatcgcaca
    26881 aaaaaataaa aatttctgtc ctttcaaata aagcccatga gtttaccgta accatggtac
    26941 aatacttttt ccccaccata catttttcaa gcattatggg cttgcaggat gggatgcctc
    27001 ctaaaccaaa cccttgcgga gcactgtatg tagcccaaat aatggcggtt gaaccagaac
    27061 atgttgcatt tattggtgat tcaggaacag atattgaggt agcgcataga agcaacatgt
    27121 acagtattgc ggtaagctgg gggtacagac cgatttcgtt actgcaacaa cactatcccg
    27181 aagcgatagc tcatacacct gacgatatac ttaccattat agaaaagcat aggtaatgtg
    27241 caataacact ttacaggtac tttggctctt tatacgaata aacggtatgg actccacctt
    27301 ctatttgtgc tgacaacgaa cgcagttcaa aacgcaattc ttctttatac agtttatcat
    27361 gaagcgttgc aatagttttt atccccatat cctgaaatgc atgcttcaaa ccttggacaa
    27421 gataaggaac atattcaaac attggtcctt tgtctacaac tgctccggaa acgccctgtg
    27481 ctaccataat tttggaatct tcagcaaagt atcgttttgc gccgcctttt tgcatagctt
    27541 caagggatgc catcccccga tagcgcttga ggcgtatgcc gttttcataa aaatattcgc
    27601 caggagcttc ttcagtccct gcaaacatag accccatcat agcagtagaa gcccccagtg
    27661 ataatgcttt tgcaatatgt ccaatggtag caataccacc atctgctatt gttggtatat
    27721 tataatctcg ggcaaatttg ctgcaacggt acactgctgt tgcctgtgcg cgtcctaccg
    27781 ccatggtaac ctgtgttgtg catattgatc cgggacccat acctattctg agtgcatcag
    27841 caccggcttt aattagattt tcacactgtt caacagttac tacattgcct gcaactacgt
    27901 caagatcagg gtatttcttc tttatatatt taatcatttc aacctgatat attgaattgc
    27961 cctgagcagc atcaatgatg acaacattaa caccggcttt tataagttct tgaagacgtt
    28021 cttttgattc atcctttgtt gaaatagcag caccgaccat taattgcttt ttttcatcct
    28081 tggaagcaaa agggaattct ttattggtaa caaggtcatt tctgctcatc aaggctacaa
    28141 gtcttccctc attatccact attggcagtt ttcctttttt tgattgtttc agtatctcat
    28201 ttgcctgtgc cagagttatg cctacacctg cagtgacaag attggtagtc attacctctt
    28261 tcaatttttt ggttctatct gtttcaaagt cgatatcacg attggtaaca atgcctataa
    28321 gctttgagtt aagtttacca tcaacagtta tgggtatacc tgaaaatcca tggctttctc
    28381 tgatttcatc gatgtgtgct atggtatgtt caggtgaaag caccactgga tcagtgataa
    28441 aaccgttttc atatcgtttt acttttctaa catgggcagc ctgctcttca attgtattat
    28501 tgtaatgaat tattccgatc cctcctaaca aagccaggct aattgccatt ttagattcgg
    28561 tcaccgtgtc cataggactt gatactaaag ggcgttttaa tgttacattg cgtgtaaaat
    28621 ttgtttccag atcaacatca tcaggattaa aatcaatata acctggaagt atgataaaat
    28681 cattatatga aagtccttga gtagaattta gaagttcttc ggctgaatag ccatcttttg
    28741 gtttcataca gtacctcaaa aagtataaag cgttatattt ttaatagtat tgttcaattt
    28801 acaagacata tcgtaattat atatgatata gcattgttta ggatttattt caatatttgc
    28861 tatataatta tttcaatata atgaacaatc aaaactatga gaacatctgc accataattt
    28921 ttatttagat caaaatctga ataaggtatt ttgtatcact gctccctgcc tataaaaatt
    28981 ttgaaaaata aagttgtatt tccacataat tattgaggct gcaatagttt ggtcaactca
    29041 acaatggaag taatggggat caatgttgtt gcaacatccg gtagagacac ttcgttgata
    29101 ttattctttg ataaaataat ggaagtaacc ccggaattag taaattctgt tattctacgg
    29161 agtatgtggc taacggggcg tatctgccct gatagtgaca gctcgccaat aataccggtt
    29221 gaatgggata tagcaaaatt ttttaagctt gatataattg ctgctgcaat ggctaaatca
    29281 catgaggtat cgttaactgc aaagcctcca gcaacattta taaatatatc aaatgagctg
    29341 agcgacagct tgacatgttt ctcaataact gccaccaaca gattaagcct gttaacatca
    29401 aatccatctg ccatccttcg gggattggta aagcttgttg cagtaaccag tgattgtact
    29461 tcaaaaagaa tggttcgggt tccttcaata gtggcgctta tgacgcttcc agggaaactt
    29521 gccttttgcg attgtacaaa taatttgttt ttatctttta cctctattaa accttgagag
    29581 gtcatggtaa aaagagctat ctcattgatt gaaccaaaac ggtttttgaa agagcgcaat
    29641 atgcgatagt cacgggtaaa atctccttca aaatataaaa cagtatccac aatatgctcc
    29701 agcacctttg gaccggcaat ggaaccatcc ttggtaatat gcccaatgag cacaatacag
    29761 gttcccgttt gtttggcaat ttcagtaagt tttgatgctg tgtagcgtat ctggcttact
    29821 gaaccaacag gtgaactaat ctctgaagag taaagtgttt gtatggagtc gataataaca
    29881 agctgaggct ggtttgtttt tattaataat tcaatattat cgatattggt aactgatgag
    29941 atgtgaattt tttcaggctt aatctgtaac cggcatgaac gcaggtatat ctgctgcatg
    30001 gtttcttcac cggaacaata cagggtggta agctgttgtg ctatctgtaa cgctaaagta
    30061 gatttcccaa tacccggttc gcctcctaaa agcagtattg aaccggggac aatcccacca
    30121 ccgcatacta aatcaaattc atatattcct gttgcaatgc gatctatttg cctggaatgc
    30181 atctctgaaa gcggatgtat ggaattttca aaagggattt catttttttg tgtaattgtc
    30241 tccgatagtt cctcaacaat ggtattccac tgaagacatt cagggcaacg tcccaaccac
    30301 ttttgaaagg ttgcaccgca ctcattgcaa acaaaaattg atttaagttt ttttgccatg
    30361 attatttttt ttgtttaaaa agcagtaatg acggatcttt ctcaagtctt gagaataact
    30421 cttttgctga gattgaggca tcacggatgt ttgtatatat ctcctcatca taaagaaatt
    30481 ttcccattgt acctctacct ttttcaaggc gatatgcaat cttatttata cttgttgtca
    30541 gttctgataa attttgcagg gtaatggtta tatcttcacg atttttttcg gatatatacg
    30601 ccatattttt ggaaatgaca ttcatctgct gcataaattg ttgtagctgt tgtgaaaatg
    30661 ttgtaatagc tgtacgtgca gtaccaaccg attggcgcac atcataacgg ttttcatcaa
    30721 ccattttatt aagattagca acaaatttat ttgaattttg gaaaatttcg gcaagtatct
    30781 gtgcaccgct tttatcctgc ataaaacttt gaaaagtttg cagcatctgg tcaaagcttg
    30841 ccgggtcaat accatatttt atgtcgcctt ctttcaggaa acctatatct tcttgcaatg
    30901 gaggtataac aacattgata tatttttcgc caatcacccc cgaggtaaaa atggaaaagg
    30961 tagctgcttt tgataatcgg taacctttat caatccacac tacgacatca gtcttttcac
    31021 cggtttgtcg tatttcatct acatatccta tctttatacc acctgcaatt ttaaccggcg
    31081 caccaactct cagatcattt aaaaacccaa aatacacatg tatacgatac cctttttgag
    31141 atatattaaa tttggttaaa atccctacca gaacaacaaa aagggtgaag cttatcataa
    31201 ccataaaccc aactcttgct tcattactca tccgcatata aacctcccaa tgaaggatga
    31261 ttgtatccaa tatacttaat ttcaatatta ttcaaccatt attcatattg agaaataaaa
    31321 atattcttag attcaacaat taagtgcaac ccttttctgt aaaaaataat gtataaaatg
    31381 attgaaaaaa acagcagtat atttataagc aaattgtcag ttgtataatt tgccaaacaa
    31441 taatgcaaat tgcatttttt atggtagcca tacaatatat ggtatacatg tctgagcaat
    31501 ggcaatgttc atctattttt ataaagaggt agccaatgag atatctggtg attggttctg
    31561 gtgggcgcga acatgcaata gcatggagat tattacatga tggaagtgct actgaagtgt
    31621 atgtagcacc tggtaatggc gggattgatg ataggtacag ggttgccata gcaatcaatg
    31681 attttgagtc aatgtatact ttttgtaaaa gcaaaagtat tgatgtcgtt gttgttggtc
    31741 ctgaagcccc gcttgtcaat ggtattgttg attttcttca caataaaggt attcgggttt
    31801 ttggtcccac agcaaaggct gcacaactag aaggaagcaa gctatttgca aagtacatca
    31861 tgcaaaaata taatgttcct actgctcaat tcagagaatt taaagataaa aacgatttat
    31921 tgcactatat tgaatcgctt actgaatatc caatagttat aaagctggat ggtcttgctg
    31981 ccggtaaggg tgttggtgtg tgcaatacaa aacatgatgc tatacagttt attaatacta
    32041 tggtcaacca tgacacaagg gtgtttgttg aggatttttg cgctggtgag gaagcctctg
    32101 tattgggaat aagtgacggg aagacagtag taccgtttat agcagcgcag gatcataagc
    32161 gtgcatttga taatgatgaa ggtcccaata ctggaggaat gggcgcctat gcaccagcac
    32221 caatagttga tagcagaatt ttaggaaggg ttcacagaga gattttacag ccggttattc
    32281 aggggatgaa taaagaaggc atcccttttg cagggatttt gtatgcgggc attatcatca
    32341 aggatgggat gattaaggtg ttagaattta atgtacgatt tggtgatccc gaaacacagg
    32401 tattgctgcc tcttttaaat ggcaaattgg gtgattatat caatgctgca atagatggca
    32461 cattatcatc aacaaatctt tcattccgga ataaacacgc tgttacggtt gttttagcat
    32521 caggtggtta tcctggttca tactcaacag ggctaccaat acatggattg gaccaaatcc
    32581 ataatgatat ccttatattt catgcaggta caaagcgtga aggcaatact attgttgcca
    32641 atgggggaag ggtgctcaat gtaaccgcca ttgctgacag ccttgaggag gcatataata
    32701 aagtatatag tgtaatacca ttgttatcgt ttgagggaat gcactataga aaggatatcg
    32761 gattcagagc tttgaaaaaa cgtgcgtaga tcaatccgct ctttttgtaa taacggtata
    32821 ttcacttctt ttataaataa aaggttaatg cctgcaagca tgctgttgca atgttctaca
    32881 tttaagatgt gattggctgc aaacgaaata ccattattgc atgggcaaac atcgattgtt
    32941 gtatccacca taattggaca tccatgagtt ctgcaaataa taggtcggta agggtatatt
    33001 ctgcatgtat gctcatgtaa gaatatacaa tattcattat ctttggtgtt tgttatggct
    33061 tcccgtacgg agggtgattg gttactatac caatgaagga tggcaaccat ctcaacgggg
    33121 aaaacacttt ccagtatgca acatccgctg cagccttctt tacattgtaa caaggtgcca
    33181 tatacagcaa taatgtcact ggtaaatgca tctatccttt ttactaattc gttgtaataa
    33241 tacagagatt tcatagagtt aatggtgcgg tattgtcatc aattgcagtg tttgttgtga
    33301 attgcttata tccatggctt tttgtaaagc ttttaaaaga tcattggaag tatacatatc
    33361 gcgaagtatg agcggctggt aaaaatattc accttttggg aaaattgcta caaatggaat
    33421 tgaacgggca ccaaggcttt gcataagctc ttctgctaat tcattttttg ttgtgatatc
    33481 tgctttataa gtatggatgt cattggactt aataaaatcg atgacctttt ttgatttcag
    33541 agtagttcct tcaaccaatt tgcaattagg gcaccagtct gcggtaaaaa ttatcattga
    33601 tacatgggaa ctattattgt gaactatcgc atcccaatcc aattgtttca atggtattct
    33661 ggatacagat ggttttgtag taaatagcat taaccctgat attataacaa ttgccaaaag
    33721 aataataaat gaagtgatac gatgtattag cggtcgggct atatttccaa atatgcccca
    33781 ttgccataat cctaacgaaa gcagcagaat aaaccataat gcctgcgtaa gggctgttat
    33841 atcaaggagg gttatcaggt aaattgaagt tgcaacaagc aaaaaaccca tgcattgttc
    33901 aaaaataatc atccattcgc caggtttagg gataaaacga acaagtgatg gatacatagc
    33961 aaggattaga tagggaagtg acatccctat accaatattg ataaatatca taaagattac
    34021 gataggatgt tgtgttaatg tccatgttag tatagctcct aaaaaggggc cactgcaggg
    34081 agtagccaga atggttgcaa ggactccttt aaagtaacta tcgatataag ggttggcact
    34141 ctgcagtttt gagggaagtg aaggaacatt aaaggtatag atgccaaaaa aagataaagc
    34201 catggaaaat ataatggcag ccattactat aataaatgtt ttatgttgaa aaagttcacc
    34261 ccagttgtaa ccggcaaatg ctgctaatgc cgctaaaata atgaaggatg ttataattcc
    34321 aagtgtgaaa agtaaaccca tactttttat ttggcgggga ttattatgtg catatgatac
    34381 aaaactaaat agtttcagac ttattactgg taatacacat ggcataaaat taagtataaa
    34441 tcctgctata atcccaaaga tgatggcatg taatatccct tttgatgaat accccatatc
    34501 gatagcattg aatgtgtaat tattgtgtga ttgttcaagg tatgtcattt gttgcggttc
    34561 tttatatggt gctatgtgtg attgcggaaa atcggctgca tcgtttttta cgttaacttt
    34621 ttgggcgata gttccagtaa ttggcgtaca ggaactatcg ttgcacagta atgcatcaat
    34681 agaaatagta aacgaatata acccttcacg attggttttg actgtaaaag ggatgcacaa
    34741 tgaggtctgg tttttataaa tatacaccgg cttgttatct atgggtgata tgtgcatcgt
    34801 tccgggaaga tagcgggtgt ttttgaattc tatgggatca tgggttgtag ccttagcaac
    34861 aagtggtttc cctgtgcctt cgcctttagg gtttgcatag atataccccc tcccgcctaa
    34921 gcgtatatta attatagcat aggcgtcagt ataaggtttt acatacagta tgtcactttg
    34981 tggctgaata gtcacatcct ctgcaaaagc atgaattgta gatataaata tactgaatag
    35041 tataatgaaa attttcacaa atggtttgta tagcatgctg ttttcctcaa tactataagg
    35101 agatatgaaa cacccttgtt ggtacaaact attgataata ctactttatt tcactatggc
    35161 aattggatga aaaaaaagtg tttaaagata ttatactatt aagaatatag caaaagttgc
    35221 cgatattgca aatagaaatt agtaaaggca caaaggaggg tgtcctatga tggcgcaaca
    35281 aattacagta gtagaacaga gcatcgaaca gcttaacaat ctgctaaaag gacttgcagc
    35341 agtaagaaca ggattaagtg agaagcttgt agaagtcaat gtaaagacac agggaaggat
    35401 agaaggatta ggaacaatta ttgatagcta tcagtaaaaa aagtagtgac tatgtattta
    35461 gtatgcttct ttttgacatt gatatctttt acaatttttg caacaatatc gctgatgatt
    35521 tcatgggtca atcttaatgt tctaataaaa tgatagaaga aatagaagaa cggacaaata
    35581 ataccatacc agaagacaat atgccaccat gtgttttgat ttgtttccca attatcccct
    35641 gatgcaggaa cgcagtttca tgttcagtga ttttcccttc cataatttct ctctctgtta
    35701 ccgcttaact ttaatccaat tctacatttg tattaatttg tttgcaagta tattgttaat
    35761 attgtcactt ttactatgat agttgaaaat atctaaaaat ggtaaacatc aatgtgtccc
    35821 aatggtatgg taattatgat attttttcat tgaatgaaat ggtattgcat aaaaacttct
    35881 aaaaattaga ttttgacgtt gccttgttgg tgtaaatatt aattaagatg cttgattaaa
    35941 ttgtgaataa ttgaaacctg attattagag gtatcaataa attgatgttg acagatagta
    36001 tatatacata atatataatt aataaaaaag ggtgaatgtg tatatggaaa aaaaattatt
    36061 aatcattgca gcaggattat tacttttatg ccagactatt tttgctgcta acaaagagat
    36121 aaatggaaca ttggggacat catatgctga taacccctca aagtatggac ttgacatttc
    36181 attacattat aattggttat tagatcctta ttttttaaca ggattggaat ccggtttttt
    36241 ctgggttcag tgggatagaa aaattggtgt aaagcaagaa ggctcagtct atgtagacgt
    36301 taaggccgat tcaaatgcat atacgattcc aataatgttt aatgctcagg tgcgattgcc
    36361 caatttgcaa agtaaattat atgttgtccc atttgcaaca gttggatttg gttactcatt
    36421 tatgatattg cattattcac gccctgattt tactgattca agaacgggga agacatatga
    36481 ttcggattca attactaaac tttttaaagg gctttcatgg gagttgcttt ttggtgtttc
    36541 attaaaacct gcaactgatt ccaaagttga tttttatgct gaatgtggtt atcgcaaatt
    36601 gccgctgaaa agtggtgatc tggaactgga tatgtcgggt tttttactta gattcggggt
    36661 aagatatcca ttgtagcatt agtagtgtaa taatgtgtga agtgttaaag ttcttgactt
    36721 tatttatacc tttattttta gttttgtaat accaatgaac atgtggggtt aatatggtgc
    36781 ctacagcata tttgtttgat cctgtttata tggatcatga tacaggatgg ggacatcctg
    36841 agcgccctga gcgcctggtg gcaatagatc agaaattacg tcagcaacca tactataaat
    36901 cactgattaa agtagagcct aaaattggtg caaaacgata tattgagctg gtacattcgc
    36961 cagattatgt taagcgagtg aaagaagaga tagaatcagg ggtccactat ctggattcaa
    37021 tggatactgc agtatgtaaa aggtcatatg aagttgcatt gttagctgta ggtggcagcc
    37081 ttaatatgtg tgatgctata atctctggag aggcaatcag agggttttgt gcagcaagac
    37141 ctccgggaca ccatgctgag tatgattatg cggcaggttt ctgcattttt aataatattg
    37201 caattgcagc aaaatatctc caggtagaac atggaataaa taaaatagct attgttgatt
    37261 gggatgtcca tcatgggaat ggtactcaac acacatttga gcgggataga actgtattgt
    37321 atataagtac acatcagtac ccacattttc caggaacggg agctcgtagt gaacggggaa
    37381 aaggggaagg tgaagggtat acattgaatt ttcctatgcc tgcagggagc ggcgatcctg
    37441 aatatatgga tgtgtttgaa aatcatattg ttccggcact tatgaagttt gaacctgaaa
    37501 tagttttact atcagcaggt tttgatgcgc ataatgctga tcctttatca tatataggac
    37561 tttcaactga agcatactac aaatttacgg ttcttatcaa gcaagctgct aatgagtaca
    37621 gtggcggaag aatgattgcc tttttagaag gtggctacga tttagaagct ttagctaata
    37681 gtgtagatat gatgatgaga gcattcattg aggattgacc atgctgttcc aatgtcatga
    37741 agttttataa agataaacta cgaatgggtg ccgcgtagtc gcccgcggtt tggtaagatc
    37801 accatccagt gaagaaaatg tttggcgata gttatcctga atactgaaca ggctacagac
    37861 aaggattgtt acaaaatccc atggaagaaa tagattctgg aatgagttca gagtgtttgt
    37921 taattcccga attacaatat tatatgccat ttagtaaata tataaataaa aattaaatta
    37981 aataaatatt caaaagtatg aaagcataat ggggtacatt ttaacaatta tgttagaaat
    38041 taattgtaaa atattttatt aataattttg taaaaaagtt gtactataaa actggtctgt
    38101 gatagaattg aaacataacg ctgttatgtt caggagggta tatgtatccg cttcttttta
    38161 caccaataac cattaataca ttacagatta aaaatagaat tgcatatcct gcgttaggat
    38221 tactttattc atatgatagc ttattaaatg acaggtatta tgaatatttc agggaaaagg
    38281 ccaaaggtgg ggctggcctt gtaacagtgg gtccagtggg gattgatata gcaggttcag
    38341 gtaggatggc attatcatta gctgatgatg ataaaatacc gtcctttaaa aaattaacaa
    38401 caataataaa aagtgaagga gcacaaacct ttgtgcaact atttcacgct ggagcttatt
    38461 catattcaaa acttactgat gatgttcaac ctattgctcc atcaagtgta tattcaaaat
    38521 attcaaagca ggtccctcgt gagatgactc ttgaagatat agagatggtt caaacagcgt
    38581 ttgtaaaagc tgcgttgcgg gcacaggaag cagggtttga tggtattgaa ataattgctt
    38641 cagcagggta tctgattacc cagtttctat cgcccataac aaataaacga acagaccgct
    38701 atggaggttc ttttgaaaac agggttaggt ttccccgcga ggtaatagaa aaagtaagaa
    38761 aagctgttgg ttttgatttc cctcttggta ttaggatggc agggaatgat tttgttaaag
    38821 gcagcaatac ggatagagaa actcctctgt ttgcaaaagt ttatgaagat gctggcgttg
    38881 atatcataaa tgtaacaggt ggctggcatg aagctaaaat tccacagttg cccatggaat
    38941 taccaaggat gggatatgct tatctggcgg ctactataaa aaaagcagta catgtgccgg
    39001 taatggcttc caatagaata gctactcctg aagcagctga aaagattttg cgtgatagca
    39061 tggcggatat ggttaatctg gggcgtgttt tgattgcaga tcctgattgg cctacaaaag
    39121 ctaaagaggg gaaaacaaat cttatacgcc cgtgtgttgg atgcaatcag ggctgtactg
    39181 atacattatt tagcgg
//
LOCUS       DAOSOX010000004        36634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1181_length_36634_cov_9.422237, whole genome shotgun sequence.
ACCESSION   DAOSOX010000004 DAOSOX010000000
VERSION     DAOSOX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 36634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36634
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1181_length_36634_cov_9.422237"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /gene="hypA"
                     /locus_tag="PLX22_00605"
     CDS             <1..340
                     /gene="hypA"
                     /locus_tag="PLX22_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOT18435.1"
                     /translation="IMSNVLDIVVQHAQQNGAVKVTKIHLLIGELSDYIPEWMQNYFD
                     FVSKDTIAENAQLVVEMVKASLQCGDCGTAFAFNKDDWQFSCPQCHSPNVTIVSGREL
                     TVKTIEIEGA"
     gene            743..2194
                     /locus_tag="PLX22_00610"
     CDS             743..2194
                     /locus_tag="PLX22_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOT18436.1"
                     /translation="MLKITFIIKHGMAVLLCLCIFQCIGYAGPIVKIEHVDITSFPIV
                     SISGKLHAIQRRNSITKDNFTVLENGKVIKELSLKHGQLDNVYIALCIDTSKSLSKNN
                     FLRLKNSAISIIKKSPDYYKFLLYKFNNEVVQLASHDDDSMVHVAKIKNLQQLGKCTQ
                     LFNCLYTAIDDLSKFDGVKAILLFTDGKDEGSDMLPDDVISIAREHSTPVYTVLPQGC
                     RKTAVVSRLSKAANGKLFIISDDINKKILQLMQSRISSGIIPFELSYNSSSTDGIGSV
                     EVRFNNGTVSDNDIQYINFKAPSFTAYRFNNVPFVVLAGIIVLLIVALVIVFIIFMNK
                     WKHIIELVSMQRIPRPVQHYYDVLFEKDEAGRKEESNLLLSSSPEYSYTSAWLVEKSG
                     PETGKKFPLIWDEVTIGHGKENTIVINDDAVSLKHAKIKKINDCYYLFDCASDNGTFL
                     NNKKLLRPKMLTDWDEIQIGRTTILFRGTKIKH"
     gene            2199..3713
                     /locus_tag="PLX22_00615"
     CDS             2199..3713
                     /locus_tag="PLX22_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT18437.1"
                     /translation="MPLKVLLCSPPVFDFYYSFHRSEPLGLLYIKAMLQQYTWLDVDI
                     YDARQKAKSKKLQTPDVFQYLNHIYMHDTSWFSLFCGYKRFGSSFDRIVGLINEKKYD
                     VVCISSLFTAYHHDVELLVKKIKEHSSAIVIVGGWAVWSMSTVEKGYADVYVKDDGEL
                     VLPELLHTIHTSNNKCMSKLPKIIKSSNGRSNEFFSNNFPERCGWYTYYGKRIANVIC
                     SKGCIYQCAFCSIHSRYTYQRRAFQSIEEEFHYLSTNGVQIINFEDDNFLYTRDYALQ
                     LLSLMKRYKEKGMNYLCMNGITASNLHAVLDDAMESGFLEFNLSLVSANDVIARRYNR
                     PLIKEMIQDIAFRCAGRVKVIVYVIAGLPGSSIDDCIQDIVLLAKLPVIIGFSPLYMI
                     PRIPMFETMGLPEDMRLCRGSALYPFGNDITREDIASLWKLCRFINKMKYLPHNPDDI
                     HLLPEHIYYYTRTCREKRWYYKSSDGTWQQGFSFQASLPEHIHVCDINGNAREM"
     gene            complement(3734..4417)
                     /locus_tag="PLX22_00620"
     CDS             complement(3734..4417)
                     /locus_tag="PLX22_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT18438.1"
                     /translation="MFKPPLTLQTTTLWNYPSQNYGNQKQGDQNYPGATPSYVIWNLL
                     MRYTNKGDMVVDPMCGSGTTIDVCNDLHRKCLGYDIHPYRADIMKADARNLPCKSNSA
                     DFIFIDPPYGDNISYSNEEQCIGKLSAKSQEYFDAMAIVIKEIHRICKPKHSMGLYIC
                     DYYNKKDGFIPIGFKLFSILLQYFKPVDIIAVARHNKTLTMGNYHRAAEEGNFFLRGF
                     NYCFIMYKE"
     gene            4564..5112
                     /locus_tag="PLX22_00625"
     CDS             4564..5112
                     /locus_tag="PLX22_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT18439.1"
                     /translation="MKKDTHECVSKCYVLYGNLVKKIIAGYFTSNEDIEDIFHDVFLK
                     LVRVDLKGDPFDPAIKHYIVKATRNTCISRIRKEMRHHNNGDSYWLSQIATIEEVNNK
                     DMAETMIDGMVVNTLYDVIKELSNDEQSLLYKKFYQNKKFSEIAAECKSTYYFIDKQL
                     KTIYKKLSLKMVRTCIDHNDIS"
     gene            5209..7365
                     /locus_tag="PLX22_00630"
     CDS             5209..7365
                     /locus_tag="PLX22_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18440.1"
                     /translation="MNYTIITICAITVIFALFVAIINIVKRYSYAVNRLFSLIAILTA
                     GMVSCLIIPYFIPDIDIVQLTRWYFGLIIIINQGFFHYTQIFPRWEKKSPLWMIITTA
                     IPGFITFVLCVATDTIITHASFIEYYFNFSYGVLIEVYLVVFIFYIMGTFFTLFYKVR
                     RLENTSFRYQLFYIFMGNHFGALIILITFIILPYAFKIYDFHSPGIASAALILLLINN
                     YAISDERYLDVKEFYFKIVFYCIVLAMTVIPSYFILVYSYRFFLRGGQIPAVAVAFIN
                     VIYLFVFTRFIQPYLEKSFKREYVRLETRFNTFIQALSQAEEFEGDPAYWDKFFSTTI
                     DSLENLFNVSSASLLMYNESEKAFILSHSIGEQTKIKRIVSTDSIVQLCEMVTTTIHQ
                     SLFYTDDTIKAFDDVLILVKSNNIEVILPIFDANGKLVAILLVGPHKKGRPYTYNEIQ
                     FMELYRVKFGNALVNSLQIEDVKTKQVAEHDQLVVNAIKTSMQPKQIPFIDHIRISSI
                     LMNNSNLGGEFCNARVLRKNLLGFLIADCSYSGVDAAVLALELYGAFESCSPVYDAPE
                     KIMQLLNWVVCTSRFTDRYATAFCFTFNSDDLSLKYLNAAFNPLILYEIRSDSFLELD
                     TKGIPIGIERDFIYESKEYRANGPCIGCIYSNGIAGAVNADGQTYSIGRIKDLIRLHN
                     SETPALIIRRISSDLNDFISNTALKEDISIVIFRIE"
     gene            7395..>8212
                     /locus_tag="PLX22_00635"
     CDS             7395..>8212
                     /locus_tag="PLX22_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT18441.1"
                     /translation="MNIDFKESAHKIISILQVQKHIAIFIKGSPDPDAIASSYFLYCL
                     CDALHVKATMFAFMPASLPSNREIISLFSIPLYIEDSIKAINLQRYSGYIVCDFQTAL
                     IDGIINAIPCLIHLDHHAPVEDDVPAHFRILTDEVSSTSTLCALIMQYISCFSQDMIH
                     LLATVLYLGIKTDSDNFNHLNKYDVMAIEYLKQLYIPSMIEKIESVPLSLYSQRLLQH
                     AMANVVIYKDWLIAGVGFVEEEHRDTIAIIADYLLENNHVELAVVYGAVYHNNHT"
     assembly_gap    8213..8312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8329..13962)
                     /locus_tag="PLX22_00640"
     CDS             complement(8329..13962)
                     /locus_tag="PLX22_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18442.1"
                     /translation="MKSLSNIIIAGAILTSITAIVISQQLSSTFHERINNYIDTHKEQ
                     TVQPSARGLDDVLILQPQQELSPFEYNSGDWTKYIIEPRYSFSIISNQIYEEEPYDIL
                     SYGSMNINVNYGKSYFTNDKYKRFDEDKPTSALIQSGLTIEQNSQLHIEGKAGKRLTL
                     YIDHDSQQQNNTYVLQYKAVNDDEVIREINAGDINIKVNNSKYAVYDDTTSKGVGIDF
                     TIKKGDLKLKAFGSVSKGVSEIERFKGNYSNTSVLLQEYQYTKGTYYQLEPFKRFDNA
                     DAPPANPYSTITWNSNPPSPATYSPYSVGIDSSGFELYADEASSENKTGLMAIYKWND
                     TSIVLGYFKKLQQGSDFTINFATGTITLLKNLKSQAKLFAVYTRNGTTQTSDPSAITS
                     HPQFSGKICVFLKYASSLHEDLNYNGVLDSGEDSNNDGMLNYDIYEIRGMYATGLQQL
                     RSDDTKLSFFNNLFPMSKTDVERLGGIIIDYNTGIIQFKLREPFKQLLSIQSQKVIYS
                     ETQSSTAYTVSQFKIGISGYSQSRGFQLKHINIIPDTVRVKINGVQLAESLYSVDPTS
                     GFFQFTNPGNPVITQDTDIEISYQYLPFEGQSQEFMGGVRADYALNKNISVGGTILYT
                     TQGSASTIPVIGNEPTQTTVVEADSTIDLTQHDISSFATKMSNSNVTIPVSFRGYVEY
                     AKSYHSINTFGKGLIDDFESIFSGLTLSMSEKDWILGSLPDSISQNMRARIYYKFYRD
                     INNPETLYGLHFTPINIPYSKKPGPYNVATGHIADSIQSENSQRSLALNFDFSSGDYI
                     SIVTQKLAVVPADLSNLEYCEIWYRSTGGTGTVNLYLDIGTINEDSDGDGIFDTEDTN
                     RNGYLDYDPNKSTSEDVGYIFNNTFPTVVGSGPGLSSITIGDGVLNTEDLNNNGMLDT
                     AEQYIRIPGLLTPISIDCSNTTWQKIRIYINKSNPSQYTSSAEAFEDILKKIYSIRLL
                     LNHDTASTGTILIDSVRFVTMHWQITEIDGTTPGDTNKASITLIDSYNDSEYAAESFA
                     LQKNNVYTSLYGDKTKKEIQKTIESALKVSYNSINSTTLIRKFYKPMDIRFYKYIHCW
                     SNFRNYTSGDSIHFKLLSSDNDYIEYAYTPQFVHAWEDIVLSLQGEITNAQFIKKEGS
                     PDLKRIIAISVTVHNTNTSGEFWLDDIYASDPITIEDTAHWYEGTIKITKPLITTQEG
                     TPVLSDIMIEYVKKGHGNDFYTIGQPYNDISEDYSQAVFSCNILPYWHTSIDYLQEQT
                     QTDSLNENVIPTRRGTTSINQLHFISTLIPPDKRIPQIDMMYNYEQFDNQQEYKQSAN
                     SYDISSNKITHQAIIGLQQTLNNILGGDLSYRLLLDTSFKDDTYKENSESIFNGFSNQ
                     KKQRQSCNVSIQYQFPHLYISPGIQVLSEEFVTYRGTVSDINPVIYHEINSNFHPPFY
                     YDDTIRFTERLKKSSLTIGLQRYQFINPSVSYEFSYFENQFKDFLPSEIWYDYGFNRS
                     HSTYGFIMNTLSLPINFSKIFPSIKGCMFTYTRSGTINEINVPYEGESTNFYNERYGV
                     KRFTSATANEICNVISYPPWYFFKGRSNYANSRDYVTYTMNTQPAIGGVHFADYNNYF
                     RLLDNAAFSINWELSPLILFLNSSVNSVSDRNGINTPAQQVVTYSIVSSVSCDLMKIF
                     SFGFFRPNKPDLPYHSAILLLEYQFNKYLRITSNIVQDEHTPTIGTTFKWDRSSVAVK
                     FGISYRTQKWYEFIPIDDRKRDNDDTIYFENMTVQPDFTNIDEGYTFSTIYETDVQFI
                     YDFFKHWYTLTAMPIFRIEYYMALNRYNYTFYTSPEPYDLYSISSNLTVNVHKNIQGS
                     IIARGILERFRNRETNSINREIVSYELGFQFSLLF"
     gene            complement(14005..14997)
                     /locus_tag="PLX22_00645"
     CDS             complement(14005..14997)
                     /locus_tag="PLX22_00645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT18443.1"
                     /translation="MKVRIKIFKSNIERKLKEKYYLKKKQWLKKWDKVLEHGHEKMTI
                     MFIPHNEQKIFNFQISRFTILFFTTLLVIVLVTSSYAIIKNTAVKREEERLLSNYKDI
                     RSQIIRFEQLTNELGQELDKIKPDIEDLYDLATNNNNSTSIWESSVVNNEDPSMRQYA
                     SILPSDIQVLRSMQKDLLCTTNTVKTIKNFIDVRNKVIYDTPSIIPNPGHITSLFGWR
                     RSPFGFDRDFHSGIDIAAPQGTEIRATAPGIVISASWGGGYGYMVRIRHKYGYETIYG
                     HCSKIIVSQGQSIKKGQIIAYVGQTGSATGSHCHYEIRIGGVAINPYPYMSKMW"
     gene            15204..15554
                     /locus_tag="PLX22_00650"
     CDS             15204..15554
                     /locus_tag="PLX22_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT18444.1"
                     /translation="MECKRCGNCCNDVRLAESPEVLQRAYKYWLQLSMINPNFSEIYL
                     IYPMLTFKYQNLSEDLPYHYSCKHFTFDSHGLGLCSIYDIRPRMCKDFPYYDGIALAP
                     EEKLSPYEGCGYNE"
     gene            15563..16669
                     /locus_tag="PLX22_00655"
     CDS             15563..16669
                     /locus_tag="PLX22_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT18445.1"
                     /translation="MPQFVEYIAFFAGGISLLVAVGQLCVPHRRLENLNLFGLFLVLG
                     LLLFQYGCILNGFIFKKPYLFYFHFTFLYCIGPLLYFAYYLVIRIEDSLPKINLLLLL
                     PSVIYCIIDIFFIRSDYNLQIAIINSLFFDNFGIYHWLRIAVIAALLLDSLYIGFLLH
                     RLFQFWKLHELTSIMVVTLIFSISSFAGLMILLASYCMRSLIGIKVSVIIMAILLIAA
                     YLVGQHKPEFLQLLIREARRKRYERTLLKGCEVESLCEKLKQLMDEERLYLDEDVTLK
                     EVANELNITAHQLSELLNVKLQMNFNTFINQYRIKEAKKMLIEESERSVLSIAYAVGF
                     NSKSSFYEAFSRFTGKTPVQYKREKKNCTYAINE"
     gene            16805..17347
                     /locus_tag="PLX22_00660"
     CDS             16805..17347
                     /locus_tag="PLX22_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOT18446.1"
                     /translation="MSKEFQYKLWAIAFYSFVAAYSYLVLTAGSGNDILFWSYEEFIF
                     GISISIILALLTFWLIPIKVTAALLNPLLYIGLILYFVGPFFLSLLIANVEVMYRIIT
                     GKIQPAIVKVDPEMDDELGIYLLANSITLSPGTLTAGYDPDKKKFFIHCLYWVKDKGY
                     KATPKDVSGFQHYFLKKLFK"
     gene            17361..17630
                     /locus_tag="PLX22_00665"
     CDS             17361..17630
                     /locus_tag="PLX22_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOT18447.1"
                     /translation="MYDFDLIFLISGLLLIIYVCIIVIRIAFSHNVPERIVALDTINT
                     LIIVIMIVLGAAQKKALYIDIGIVYGIISFIGTLYIARYLIDEKR"
     gene            17633..18040
                     /gene="mnhG"
                     /locus_tag="PLX22_00670"
     CDS             17633..18040
                     /gene="mnhG"
                     /locus_tag="PLX22_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOT18448.1"
                     /translation="MDTIICIFLVIGLLVNGVSIIGLMRFPDIYTRLHAATKTTTFGS
                     IFVVLAVGVKNIWYYDATSITIIIHSIVALLVIIFTNPISAHAIARASLLSGIKPFGV
                     VINEYDYKSQIKTVDDLEMDKISEGIPDEEGTL"
     gene            18037..18279
                     /locus_tag="PLX22_00675"
     CDS             18037..18279
                     /locus_tag="PLX22_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOT18449.1"
                     /translation="MIYYFFLVISIALVITALLSIYFRDLLAAIVSYSVFSLVLTVLY
                     FLLDAPDVAIAEAGIGAALTTCIFVIAIRMTRRREE"
     gene            18276..19103
                     /locus_tag="PLX22_00680"
     CDS             18276..19103
                     /locus_tag="PLX22_00680"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOT18450.1"
                     /translation="MRLISVVAIVVIFGFIFVGILHLHPIGEPIGNPRILYERQPLGI
                     DTQGVTIMDDYFLRNGQVETGSNNIVASIVFDYRGFDTLGEATVLFLVVSSISMLFFT
                     FLKSKSIISAGVSKAGQFPKIESRIITFGTFLMYIMILSFGIYLVVHGHISPGGGFQG
                     GSVMASATALLLISFYITRHMQRTRKIFSLFESLGLTIFIGMGFAGIGISFLYNFLAH
                     GTTVFGKIIPFGPNPGYLISAGILPWLSLAVGIEVFFGLSLILVTLFHASGVKNNSL"
     gene            19113..19469
                     /locus_tag="PLX22_00685"
     CDS             19113..19469
                     /locus_tag="PLX22_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOT18451.1"
                     /translation="MSAIILYITFVLLFTIGLFALIFKRNLIKIVIALNILDSGMNLF
                     FITLGYRHNATSPIFTLLPWDTHITMVLPTPQALILTNIVIGFATTALMLGVAILTYR
                     NNKTLDTRKLRGVEEL"
     gene            19466..20992
                     /locus_tag="PLX22_00690"
     CDS             19466..20992
                     /locus_tag="PLX22_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT18452.1"
                     /translation="MIKHVPVLLVTIPLVVAFMLPIIGMVNRTIRTITAGVAIVVIMV
                     LSYLLIQDVMIYKKIIHYMVGSPDPNALLEHGLTFPIRIALKIDQFSLLFIIMVTTLS
                     FIFYLFSSQYIEENERKNYFTVLYVCMVAGMMGMLITNDLFTFFVFLEILSISSSALV
                     SFRTERKHPPYAGYKYMFVSSIATSFFLLGIAFLYAQYGSFNIDYLKKVMNGTVLDKI
                     AIVMLILPLAMKCGVVPMHMWIPDTYSEAPAPISSMVKLGSLICLYGLFRIAFTFGLS
                     RAQVSYELGMLFILFGVLSMFVGVTQALIQKDVKRLMAFHAVSQVGYMLLGVGVGLSV
                     IKSGDASFAIYGKIAIIGGLFHVVNNALYKGLLFLTSGIIVRHYKTRDLNIMHGLAHY
                     DKFTALVFMIAALAISGIPPLNGFVSKLIIYESVFHYNPLLTLIAIFVSVLTLASFMK
                     VFYSAFLGMPPERRPVEQPILVKCSMIILSIVIIVIGLFPQGIIKLLIEPAVNVLSTF
                     "
     gene            21005..21343
                     /locus_tag="PLX22_00695"
     CDS             21005..21343
                     /locus_tag="PLX22_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18453.1"
                     /translation="MHEFELEYLNWMPLLWVCIFLIIMVLFIIIGKIFFRNDFDEMSE
                     QGLPFYSGTSDKMLQLITASNLYWGFKHTLQSYFDGLNRLFISDIGDFIQWFIIIVAI
                     MLLIITGALM"
     gene            21391..21765
                     /gene="nuoB"
                     /locus_tag="PLX22_00700"
     CDS             21391..21765
                     /gene="nuoB"
                     /locus_tag="PLX22_00700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOT18454.1"
                     /translation="MNTGSCNACDIEILAMLMPRYDAERFGIKLVGSPRHADILLVTG
                     TVTEKIKDKILQVYQQVPKPKLVMIIGSCGATKGVFQESYAMAGPFDTLLPVDVYVPG
                     CPPRPDAIIFGVAKAWEKLEVL"
     gene            21789..22283
                     /locus_tag="PLX22_00705"
     CDS             21789..22283
                     /locus_tag="PLX22_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT18455.1"
                     /translation="MDVQSIVTIIQEKLGNHLQASRLKVKNYHGGIMRLHLWISIDRE
                     AFHFTVNTLKSLGRLHLSTAMAYKEYEDRIELIYPFALKMDDTHYSELMIIASVDLPN
                     NDLTIRTITDIVPGALFMEREVMEMLGIVIEDIPDSRRLFTPSSLQDGYFPLRNGKNK
                     EDSK"
     gene            22280..23509
                     /locus_tag="PLX22_00710"
     CDS             22280..23509
                     /locus_tag="PLX22_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOT18456.1"
                     /translation="MKSDRVYPLNIGPIHPAFKEPIKFTFRIEGEQVTGVDIDFGYTH
                     RGIEKIALERNYIQIIYLLERVCGICSFSHPLAYCLAIEDVAEIQVPDRARYIRTIIG
                     EMERLHSHLLWTGVAAHEIGFDTLFMYTWNIREKVLDCLEELTGNRINYGMLTIGGVR
                     RDVTPENSETITRMLEYYMELYDRASEILLDDLTLRARTEGIGILSREQAEMFCAVGP
                     TARGSGLLKDVRIDYPVNAYYDIPWLNPISPRDLGKEPIGDVYDRMAVRVLEIKQSLN
                     IIQFCMENLPEGDIHSGDGAVKIINTLKKLEGEGIGRYEAPRGEVCHYVILDNQEHPI
                     QLKVKAPTYSNGFTWIPMLTNIEIADIPIVIASIDPCVACADRMTFIRKGGDEERYNW
                     EYLHQKSIKKYKRSVNQ"
     gene            23506..24519
                     /locus_tag="PLX22_00715"
     CDS             23506..24519
                     /locus_tag="PLX22_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT18457.1"
                     /translation="MIMVLAIALIITIVGLILGLLLKGIDRKIAAYFQSRIGPPLLQP
                     VYDIIKLVYKQTIIPVTAVRWLFSSAPVIALASSVLLLLYISMPYIFYITGIDTTLQG
                     DLIVVLYCILIPSIAIVTGAFSSGSPYAAIGAQREMVILMSIELPIAVVIISVAWHVA
                     DSMADPFAMSSLFEYPLWHNAGAFGVLGGILLFFAMELVLPAEMSRVPFDQAEAETEL
                     AEGVSVEYSGKLLAFILLTDAFKALALSMLVIILFFPYTLSQLFGISFQMYGHRVAPF
                     VEIAFLLFKIIVIYSFGITFIRVIMARLKISQVARFFLFAITAISIIGLILIMLDPNM
                     RII"
     gene            24530..24994
                     /locus_tag="PLX22_00720"
     CDS             24530..24994
                     /locus_tag="PLX22_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT18458.1"
                     /translation="MLGMVYKVIEQIFSKKSTNCFPTKYIPNNTKQYLNQGIIHPPIV
                     VKEGFRGRLEYDFQSCTGCGLCSKVCPANAIELYSAEVNNKKTKRIVIYLTRCTFCKE
                     CVDICPRNSITMTKAFCMADYKKYGNSLVVGSEYRQQCEIKDHTDVVQKKTN"
     gene            complement(25064..25789)
                     /locus_tag="PLX22_00725"
     CDS             complement(25064..25789)
                     /locus_tag="PLX22_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT18459.1"
                     /translation="MHIKMKEKQITKYVKTVGKAINTYTMIEAGDTICIALSGGKDSL
                     VCMDALYRRLSYLPIHYTLYCCHVNVTNFNNKPINILQSYCSIYNIPLIIKDITLDLS
                     IKGSSPCFVCSWYRRKALFETAAQLNCKKIAFGHNQDDIITTFFLNMLYHGKLSTMPP
                     KLKMFNGALEIIRPLAFLTEAQIKGYIKALPLSPIENECPYKDCTKRYQMKELLKNLN
                     KTIKGAQTNIFHSLSHWDDDYLL"
     gene            25896..26261
                     /locus_tag="PLX22_00730"
     CDS             25896..26261
                     /locus_tag="PLX22_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18460.1"
                     /translation="MARCMIVDDSSFMRRIIHQTLEEAGHQVVAEVDNGLDALSKYKE
                     VKPDVVTMDITMWGKDGLEAISEITSDYPDAKIIIISALNERTLKINEKDINARLFLS
                     KPFEKQELLDAIEKVLNNG"
     gene            26254..27297
                     /locus_tag="PLX22_00735"
     CDS             26254..27297
                     /locus_tag="PLX22_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT18461.1"
                     /translation="MDEQLLQLYKKNAIADNAYMIADGTVFLFVTEADKYVLQGKNVI
                     IGATEPVLNSKTYENIHRLETALAVKGTTIKKIPANTIIQSLNNTAFTMNLAIVEAKV
                     VVLTNDILKKNMQIDSQLVKERNICIEYYTIVHSLQLEYEKRKLPWLKDLIDKFKTSI
                     TFKKGEAFSRANESLRIDTPRELSANMIEFPPGSLLCEEGSVGDAMYILESGQLEVYI
                     QNSKVATISEKSTVIGEIAMLLSMHRTATLKAQNTVVVTKITREDLRRSTSPELTQAL
                     LVSLAKKHHANVLHIMDINEKKALAYADSPRQELIKTEAYRYQNELSQLKYTLEKLIQ
                     SKQADYLEYILNK"
     gene            complement(27301..27837)
                     /locus_tag="PLX22_00740"
     CDS             complement(27301..27837)
                     /locus_tag="PLX22_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOT18462.1"
                     /translation="MKIEITILGLLMEENLYGYEIKKRIVERLEDYVDIKFGSIYYAI
                     KKALDNGWVKKIGTEKEGGNPERYIYQILPDGRKYYKKMLKQYFDHNFIHFDIDIVLM
                     FLNSLSPEQKEQFIEDRVEHIKDKMNALKEKIDTELKNNSPNSQLHVYTYLENHLKAE
                     LNWLKSLKKGEVLTEVEQ"
     gene            complement(28337..28921)
                     /gene="ruvA"
                     /locus_tag="PLX22_00745"
     CDS             complement(28337..28921)
                     /gene="ruvA"
                     /locus_tag="PLX22_00745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT18463.1"
                     /translation="MIAFLKGKKISIKPTKVIVDVNGVGYEVTIPLSTYEKISSVATI
                     ELYIAMVVRENSVALYGFSSEEEKQMFAILTSIPGVGPSIAISILSSMSPHYITNAVK
                     AENILAFTAIPGIGKSKSEKIIFELKRKLRKLEATVQHPATTSPDRQDAIDALVSLGF
                     DEKLAMSTISSIITSNPDCDLETIIRLSLKQLSQ"
     gene            complement(28946..29434)
                     /gene="ruvC"
                     /locus_tag="PLX22_00750"
     CDS             complement(28946..29434)
                     /gene="ruvC"
                     /locus_tag="PLX22_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT18464.1"
                     /translation="MATIMGIDPGYGRCGWAIVTDRLTYIDCGVIETTPGDEGERLFT
                     IYQSVGDLIALYQPQSCGLEKLFFSKNTKTALSVVQVIGVCKLILTQHAIPYSEYTPM
                     QIKQSITGYGRSSKEQLSTMLRKILKCDTIHNYDDAFDAIAIALCHCLATPERSLLHQ
                     KV"
     gene            complement(29440..30186)
                     /locus_tag="PLX22_00755"
     CDS             complement(29440..30186)
                     /locus_tag="PLX22_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT18465.1"
                     /translation="MSGHSKWANIKHRKAAQDAKKGKIFTKFIREITVAARVGGDDLN
                     SNPRLRTAVLKARENNMPMENIERAIKKGTGALDGSTYEEVRYEGYGPGGVAIMVDCM
                     TDNKNRTTPEIRTIFSKNGGNLGESGCVSFLFIRKGVINIDANATNEDKVMEILLDYD
                     VEDIKTEDTTIVVYTSPDKFNDILEIIRSHNLPTLNSEITYIPKTTINLEGQKASQCL
                     RLIEALEDHDDVQDVYANFDIPDDIINSMS"
     gene            complement(30312..31589)
                     /locus_tag="PLX22_00760"
     CDS             complement(30312..31589)
                     /locus_tag="PLX22_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18466.1"
                     /translation="MNTKLPILITLLLSFFMNSYLFGQNYKDAIHQKKFSEALTIIQS
                     KLDDIYSKRSIEKQIPTTYVAIEKIEEGVDLQKIFSERKIQPYFIENNDTLYALHADA
                     ALCYQNIYKYHEALQHYFQALRFTTIGEKDHTIFYSIAQIFKKINKFNAYVLYLEEAY
                     EIKPDNYDYSLELSLALSSGKNKKKALFHLNRYIASKGDSVPPNLYLIAANCYESIGD
                     YIKASEQYTLYLHHKPNDYAVLFALGYLAYTKISDMKLAYKSFINAIPLFNENDWVLK
                     GLSYTMLGDIECMDLNYKNGLEYYLQSIAISENMKKIIDEKKANINTINDKINVIKST
                     LLANKDIALYPQYQTLMDELGNNELELRQLEHEYSKLNAGLLSFKIAVAHENMMQFAQ
                     AIEWYNKTIEYGIKIRESSKKIEKLQLKISRGF"
     gene            complement(31657..32271)
                     /gene="pth"
                     /locus_tag="PLX22_00765"
     CDS             complement(31657..32271)
                     /gene="pth"
                     /locus_tag="PLX22_00765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT18467.1"
                     /translation="MKLIVGFGNPGEEYFNNRQNIGFKVVDILGNNENIDIRVKKKKS
                     IIGRGKISGEDVVLLKPQTFVTLIGESVLYIASFLRINVRDIICVLEDSSLPLGEIRV
                     DSIMSKLKHPGIESMTKALKSDRFAKVRIGIGYPHKGITMEEHLLNDFSDDENFILID
                     VLNKAEEVVRMLISHSIEEIQNKYNPEGALKIKKRQLPRIRIRR"
     gene            complement(32297..32920)
                     /locus_tag="PLX22_00770"
     CDS             complement(32297..32920)
                     /locus_tag="PLX22_00770"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOT18468.1"
                     /translation="MEAQVLPIKTRSEIGKNANHRLRAQGYIPAVLYSHGVSQAVMVE
                     KKNFFKIFKGHISENLLIDLQFNDHSSAPVKAFIKDYQRHPITDEILHLDFFKVTMTE
                     TITTKAPIEIIGTSIGVKQGGILEVIEREIEVECLPADLPEKVQIDVTNLAIGQSIHV
                     KDIIAPNGVKILSSPESVIVAVLAPHKATEEVAQPAGEQPQETVEEQ"
     gene            complement(32996..33943)
                     /locus_tag="PLX22_00775"
     CDS             complement(32996..33943)
                     /locus_tag="PLX22_00775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOT18469.1"
                     /translation="MIYPIKLFSGTSNYALAQDIAHYLGIELSEIEIKKFKDGEISVK
                     ILENVRSVDVFLIQSTSNPANDNIMELLLMVDAAMRASAQRITAVIPYYGYARQDRKV
                     EPRVPISAKLIANMLHAAGVNRVLTMELHSDQIQGFFDIPVDNLFASPVVIEYVNELN
                     IQNPTVVSPDAGGTERARFLARNISAELAIVDKRRPEPNKSEVMNIIGEESVKNHNCI
                     IIDDMIDTAGSIAQAANALKKSGAKDIYCTATHAVLSDNAIEKLKKAEFKEIILTNTI
                     DIPEHKILPNMKILSMAPLFGEAIRRIHNGESVSSLFIR"
     gene            complement(33951..34700)
                     /locus_tag="PLX22_00780"
     CDS             complement(33951..34700)
                     /locus_tag="PLX22_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT18470.1"
                     /translation="MNNCKAVILAAGKGVRMQSSLPKVLHRLAGKPLVQHVIDNLISA
                     GISYNDIIVVVGYKGDDVISAIDNNISFVWQIEQLGTGHAVMQTEPQLGTYNGCLLVA
                     CGDVPLIKPETFIKLYNAVNDHSTGAAVLTMELENPCGYGRIIRGEYDNLIRIVEEKD
                     ADPIQRNIKEVNTGTYAFKSPILFQGLKTITTDNAQREYYLPDVLTFIRKSGYDVKII
                     KLDDPIEGTGVNSKEELIKLGSIITKTFIKK"
     gene            complement(34725..34796)
                     /locus_tag="PLX22_00785"
     tRNA            complement(34725..34796)
                     /locus_tag="PLX22_00785"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34762..34764),aa:Gln,seq:ttg)
     gene            complement(34834..35718)
                     /gene="ispE"
                     /locus_tag="PLX22_00790"
     CDS             complement(34834..35718)
                     /gene="ispE"
                     /locus_tag="PLX22_00790"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT18471.1"
                     /translation="MIESIKAYAKINLHLEVLNKRPDSYHNICSIMAKINIFDLLQLE
                     GCELCNPNTIDITIRCIGGQNAAIVKELPIEQNLITKAIRLYCSTAHIGSRVTIAIQK
                     EIPSGAGLGGGSADAAAALMLCNNVFNKFSHNELMALAAEIGADVPFCLNGNIALCEG
                     KGEIVTPLKKIPLPDAVVIVFNDVHSSTKEAYTALMRSFAPVLSKKEAVLSLWDKSDV
                     GALLNIFKNDFQDIVFDNNPQLREIYRTLQDFAPDFIHMTGSGSALCALFHDIGKANE
                     VFLTLKPHYSQVFVSNLL"
     gene            36282..>36634
                     /locus_tag="PLX22_00795"
     CDS             36282..>36634
                     /locus_tag="PLX22_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18472.1"
                     /translation="MKLYLIPLLVACLFFAGCSPTEVFFSSYSAQCGVFDCSTIDFTK
                     EKPVELKGEWKYRMLEDSPAFARPDYDDSEWDYIIIPNYWNSEKFKGDGYGWYRLKVK
                     VNKSFWETSPAIYVPG"
BASE COUNT        11230 a         5903 c         6924 g        12477 t
ORIGIN      
        1 tatcatgtcc aatgtgcttg acattgtggt gcagcatgca cagcagaatg gggcggtgaa
       61 ggttacaaaa attcatttat taattggtga gctatctgat tatatccctg aatggatgca
      121 gaactatttt gattttgtaa gcaaggatac aatagccgag aatgcccagc tggttgtgga
      181 gatggtgaag gcatcgttac aatgtggtga ttgtggcact gcatttgcat ttaataaaga
      241 tgactggcaa tttagttgtc cgcaatgtca ttcgccaaat gtaacgattg tttcaggcag
      301 ggagttaacg gttaagacta tcgagattga gggagcatga agcatgataa ctgttgtcat
      361 ggttgtttgc gataatagat catatagaca ggaatgtaaa gacctttcag gaaaagcaat
      421 tgagcaatag gaaccatgca atacttttaa acataaaaca tgcaatgacg tcatggataa
      481 ttgcagaccc tcaatacgtc aacaatcatt aatttgtcag gtaatgctaa agctgttaat
      541 gagaatctat cgattatatg gatatgctac cgaacaccat acaattgttg caaggacagg
      601 aaaggattgc ttctattgct tccatgaata aaatttgatt gcattcacac tttattcata
      661 ccgatactat tactatatcg tagtcgttca acgctgtggt taacaataaa gagtacaata
      721 ggaattgatg gaattgtagc ctatgttaaa aataacattc atcataaagc atggtatggc
      781 tgttttgctt tgcttatgta tattccagtg tattggatat gcaggcccta ttgtaaagat
      841 tgaacatgtt gatataacat cattccccat tgttagtatt tccggtaagc tccatgcaat
      901 ccagagaaga aatagtataa caaaagataa ttttaccgta ttggagaatg ggaaggttat
      961 aaaggaattg tcactgaagc atggacagct ggataatgta tatatagcct tatgcattga
     1021 tacgagtaaa agcctttcaa aaaataattt tttgcgttta aaaaatagtg ccataagcat
     1081 aataaagaaa agtccggatt actataaatt tctactgtat aaatttaata atgaggttgt
     1141 gcaattagcg tcgcatgatg acgatagtat ggtgcatgtt gcaaaaataa aaaatttgca
     1201 acagcttgga aagtgtaccc agttgttcaa ttgtttatat actgctattg acgatctttc
     1261 taagtttgat ggagtcaagg caatactgct ttttactgat ggtaaggatg aaggaagtga
     1321 tatgcttccc gacgatgtta tcagtattgc gcgggaacac tcaacccctg tttatactgt
     1381 actgccgcaa ggttgcagga aaacagctgt tgtgagtcgt ttgagcaaag cagccaacgg
     1441 taaattgttt ataataagtg acgatatcaa taaaaaaata ctccagctta tgcaatccag
     1501 gatatcatcg ggcataatcc cttttgagct gagttataac tcatcaagta ccgatggtat
     1561 tggctctgtg gaagtcagat ttaacaatgg tactgtcagt gataacgata tacaatatat
     1621 aaactttaaa gcaccctcgt ttacagcata taggttcaat aatgtgcctt ttgtagtact
     1681 ggcaggaatt attgtgctgc ttatagttgc actggtaatt gtttttatta ttttcatgaa
     1741 taaatggaaa cacattattg aacttgtcag tatgcagagg ataccacggc cagtacaaca
     1801 ttactatgat gtactttttg aaaaagatga agctggaaga aaggaagaat caaacttatt
     1861 gctgtcaagc tcacctgaat atagctatac cagtgcatgg ctggtagaga aaagtggtcc
     1921 tgaaacagga aaaaaatttc ccttaatctg ggatgaggta accattgggc atggtaagga
     1981 aaatacgata gttatcaacg atgatgctgt gtcgctgaaa catgcaaaga taaaaaagat
     2041 taatgactgc tattatctct ttgattgtgc cagtgataat ggcacctttt taaacaacaa
     2101 gaagctcctg cgtccaaaaa tgctcaccga ttgggacgaa attcaaatag gaagaaccac
     2161 aatacttttt agaggaacaa agataaaaca ctaatgccat gccattaaaa gtattactat
     2221 gctcaccgcc ggtctttgat ttttattata gcttccatcg ttctgagccg ctgggccttt
     2281 tatatatcaa agcaatgcta caacagtata cctggctgga tgttgatatc tatgatgcac
     2341 ggcagaaagc aaaatcaaag aagcttcaaa ctcctgatgt ttttcagtat ctgaaccata
     2401 tctatatgca cgatacctca tggtttagcc tgttttgtgg atataagcgt tttgggtctt
     2461 cgtttgacag gatagttggt ttgattaatg aaaaaaagta tgatgtagta tgcatatctt
     2521 ctttatttac agcatatcac cacgatgttg agttgctggt taaaaagatc aaggagcata
     2581 gcagtgcgat agttattgtg ggaggctggg ctgtatggag tatgtccacc gttgaaaagg
     2641 gatatgctga tgtttatgta aaagatgatg gcgagcttgt attgccggag ctgttgcata
     2701 ctatccacac ctcaaataat aagtgtatga gtaaacttcc caaaattatc aaatccagca
     2761 atggcaggag caatgaattt tttagtaata acttccctga gcgatgtgga tggtatacat
     2821 actatggtaa aaggattgcc aatgtcatct gtagcaaggg atgcatctat caatgtgcat
     2881 tttgttcaat acattcgcga tatacctatc aacgcagagc attccagtcc attgaagaag
     2941 aatttcacta tctctcgact aacggtgtgc agataattaa ttttgaggat gataattttt
     3001 tatataccag ggattatgct ctacagttac tatcgctcat gaaacgttac aaagaaaaag
     3061 ggatgaacta tctttgcatg aatggcataa ccgcttcaaa cctccatgca gtacttgatg
     3121 atgccatgga gtccggattt ttggagttta acctatcgct tgtttcagct aatgatgtta
     3181 ttgccaggcg ctataaccgt ccgctgataa aagaaatgat tcaggatata gcctttcgat
     3241 gtgcaggcag ggtgaaggtt attgtttatg ttatagcagg tttgcctggc agcagtattg
     3301 atgattgcat acaggatatt gtattgctgg caaagctccc ggtaataatt ggattttcac
     3361 cactgtatat gattccgagg atacctatgt ttgaaactat ggggcttccg gaggatatga
     3421 ggttgtgcag agggagcgcg ctctatccgt ttggcaatga tatcacacga gaggatattg
     3481 caagtttatg gaagctgtgc cgtttcatta ataaaatgaa atatctccca cacaaccctg
     3541 atgatataca tttgttaccg gagcatatct actactacac cagaacatgc agggagaaaa
     3601 gatggtatta caaaagcagt gatggcacct ggcagcaggg tttttcattc caagcctcgt
     3661 taccagaaca tatccatgtt tgtgatatta atgggaatgc tcgtgaaatg tgatgctgta
     3721 gttttttgct gttttactct ttatacataa taaaacaata gttaaatccc cgaagaaaaa
     3781 aattgccttc ttcagcagcc ctgtgataat tgcccattgt cagtgtttta ttatgtcgtg
     3841 ctacagcgat gatatcaaca ggtttgaagt attgcaacag tatggagaat agtttgaatc
     3901 ctataggaat aaagccatcc ttcttgttat aataatcaca aatatataat cccatggagt
     3961 gttttggttt acaaatgcga tgtatttcct taataactat cgccatggca tcaaagtact
     4021 cctgactttt agcgctcagt ttcccaatac actgttcttc gttggagtag ctgatattat
     4081 cgccatacgg cgggtctata aagataaaat cagcgctgtt acttttacag ggaagattgc
     4141 gtgcatcagc tttcataata tcggctctat acggatgaat gtcatacccc agacatttcc
     4201 gatggagatc gttgcataca tcaatggttg tcccgctgcc gcacattggg tcaacaacca
     4261 tatcaccctt gtttgtatac cgcatgagca aattccatat gacatatgaa ggggttgcac
     4321 ccgggtaatt ttgatcaccc tgcttttgat tgccgtaatt ctgggagggg tagttccata
     4381 atgttgtggt ctgtaatgtt agtggcggtt taaacatgat agtaacctca agagctatta
     4441 tttacaatac tatatcaaaa atttttctca ttttcagata aatcgcaaaa taagaagatt
     4501 ttaatagtaa actatgctat tacaaacgta aaaatgtaat atttcaatac tttgggggat
     4561 gacatgaaaa aagatacaca tgaatgtgtt agcaaatgct atgtgctcta tggtaatctg
     4621 gtgaaaaaaa taatagccgg ttactttaca tccaatgagg atattgaaga tatcttccat
     4681 gatgtatttc ttaaattagt gagggttgat ttaaaagggg atccgtttga tcctgctata
     4741 aagcattata ttgttaaagc aacacggaat acctgcatca gccgtattcg taaagaaatg
     4801 cgacaccata acaatggcga cagttactgg ctatctcaaa ttgcaactat tgaagaggtt
     4861 aataataaag atatggctga aacaatgatt gatggtatgg tagtcaatac tctgtatgat
     4921 gttataaagg aattgagcaa tgacgagcaa tcattgctgt acaaaaaatt ttaccagaat
     4981 aagaaatttt cagaaattgc cgcagagtgt aaatcaacat attattttat tgataaacaa
     5041 ttgaaaacaa tttataaaaa gctttccctg aagatggttc ggacatgtat agaccataat
     5101 gatatatcat aaattataaa ttagcttgaa atttatcgat aattattgta aaagtacttc
     5161 aattttatat catgggatta tatataatat attctggatg gtttagcttt gaactacact
     5221 ataattacta tctgtgcaat tactgttata tttgctctct ttgttgccat cattaatata
     5281 gtgaaaagat attcttacgc agtaaaccgc ttgttttcgc ttattgccat tctgacagcg
     5341 ggtatggttt catgtttaat tataccatat tttattcctg atattgacat tgttcaactg
     5401 acgcgctggt attttggttt aataataatt attaaccagg gcttttttca ttatacacaa
     5461 attttccctc gatgggaaaa gaaaagccct ttatggatga ttataacaac tgctatacca
     5521 ggctttatta catttgtact ttgcgtggca actgatacaa taattaccca tgcatcattt
     5581 attgagtatt atttcaactt tagttatggg gtattgattg aagtatatct agttgttttt
     5641 attttttata tcatgggtac tttttttaca ttattttata aagtgaggcg attagaaaat
     5701 acttcattta ggtatcaatt gttctatatt tttatgggaa accattttgg tgcattgatc
     5761 attttgataa cgtttataat tttaccgtat gcttttaaaa tatatgattt tcacagccct
     5821 gggattgcat ccgctgcctt gatattgtta ctgataaata attatgcaat ttcagatgaa
     5881 cgctatcttg atgttaagga attctacttt aaaatagttt tttattgtat agtattggct
     5941 atgacggtaa ttccatcata ttttattctt gtatatagtt ataggttttt tttgagaggt
     6001 gggcagattc ctgccgtagc tgttgctttt atcaatgtta tatatttatt tgtatttaca
     6061 cgttttatcc agccatatct ggagaaatcc tttaaacgag agtatgtacg tctggaaaca
     6121 cgttttaata catttattca ggcattatct caggcagaag aatttgaagg ggatccagca
     6181 tattgggata aatttttttc cacaaccatt gatagtctgg aaaatttgtt taatgttagt
     6241 tctgcatcac ttttgatgta taatgagtca gaaaaagcat ttattttaag ccacagcatt
     6301 ggtgaacaaa caaaaattaa aagaatagtg tctaccgatt ctattgtgca gttatgtgag
     6361 atggtcacaa caacaatcca tcaatcatta ttttatacgg atgacactat caaagctttt
     6421 gatgatgtcc ttatattggt aaagagtaat aatattgaag ttattcttcc tatttttgac
     6481 gcaaatggaa aacttgtagc gatattattg gtgggtcctc ataaaaaagg gcgcccatat
     6541 acctataatg agattcaatt catggaatta tacagagtga agtttggtaa tgctttagtc
     6601 aattctttgc agattgaaga tgtgaaaaca aagcaggttg ctgaacatga tcaattagtg
     6661 gttaatgcta ttaaaacttc aatgcaaccc aaacaaatac cgtttatcga tcatatcagg
     6721 ataagttcta tcttgatgaa taattcaaat ctgggtggtg aattttgcaa tgcgcgagtt
     6781 ttacgcaaaa atttgctggg atttttaata gctgattgtt catatagtgg agtagatgct
     6841 gctgtattag ctctggagtt atatggggct tttgaatcgt gttcacctgt ttatgatgct
     6901 cccgagaaaa taatgcagtt attaaactgg gttgtgtgta cctccagatt tactgataga
     6961 tatgctactg cattctgttt cacatttaat tcagatgatc tatcgttaaa atatctcaat
     7021 gcggcattta atcctctcat tctctatgag attcgttctg acagcttttt agaattggat
     7081 accaaaggga tacccatcgg gattgaacgc gattttattt atgaatccaa agaatatcga
     7141 gcaaatggac catgtatagg ctgtatctat tccaatggga ttgccggtgc tgttaatgct
     7201 gacggacaga catatagtat tggcaggata aaagatttaa tccgtctgca taatagtgaa
     7261 accccggctt tgattatacg acgtatttca tctgatctca atgattttat aagtaataca
     7321 gcattaaaag aagatatcag cattgtgatt tttcgtattg agtgaatgca tatcgttatt
     7381 ttcgaaaatt ttatatgaac attgatttta aagagtcagc tcataaaata atatctatcc
     7441 ttcaagtgca aaaacatatt gcgattttca ttaaaggatc gcctgaccct gatgcaatag
     7501 cgtcttcgta ctttttgtat tgtttatgtg atgcattgca tgtcaaagca acgatgtttg
     7561 cttttatgcc tgcatcgtta ccatccaata gagaaattat atctctattt tctattccat
     7621 tatatattga agattccata aaggctatta acctgcaacg ctattcagga tatattgtat
     7681 gtgattttca gacagcttta attgatggaa taataaatgc tattccctgc cttatccatt
     7741 tagatcacca tgcaccggtg gaggatgatg tacctgctca ttttcgtatt ttaactgatg
     7801 aagtatcatc gacaagcacc ttatgtgctc tcattatgca atatatatca tgcttttcac
     7861 aggatatgat acacttactg gcaacggtat tatatttggg aataaagaca gattctgata
     7921 attttaatca tttaaacaaa tatgatgtaa tggcgataga atatttaaag cagttgtata
     7981 taccttcaat gattgaaaaa attgagagtg tcccgctgtc actgtattct caacggttac
     8041 tacaacatgc aatggctaat gtggtgatat ataaagactg gctaatagca ggtgttggat
     8101 ttgttgaaga ggaacatcga gacactattg ccataattgc cgattacctt ttggaaaaca
     8161 atcatgttga acttgcagtt gtttatggag ctgtctatca taataatcat acnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaagaaa agtagatttt aaaataacag
     8341 gctaaattgg aatcctaatt cgtaagaaac aatttcacgg tttattgagt ttgtttcccg
     8401 gtttctgaaa cgttccagta taccacgagc tataattgat ccctgaatgt tcttgtggac
     8461 atttacagtg agattggatg aaatactata aagatcatag ggttccggcg aagtgtaaaa
     8521 ggtatagtta taccgattaa gggccatgta gtattcaatg cgaaaaatgg gcattgcagt
     8581 aagcgtatac caatgtttaa aaaaatcata aataaattgt acatcggttt catagatagt
     8641 tgagaatgta taaccttcgt caatattggt aaagtcaggc tgaactgtca tgttctcaaa
     8701 gtaaatggta tcatcattat ctcgcttcct atcatcaatc gggataaatt cgtaccactt
     8761 ttgtgtgcgg tagcttatgc caaatttaac agcaacactt gatctgtccc atttaaaagt
     8821 tgtccctatg gttggggtat gttcatcctg tacaatattt gaggtaatac gcagatattt
     8881 attaaactga tattccagta gcagtattgc agaatggtat ggtaaatcag gtttatttgg
     8941 tctgaagaat ccaaaagaaa aaatcttcat gagatcacat gaaacagatg aaacaatgct
     9001 gtaggttaca acctgttgag caggggtatt gataccattt ctgtctgaaa ctgaattgac
     9061 acttgagttt aaaaaaagta taaggggcga cagttcccag ttaatggaaa atgctgcatt
     9121 atcaaggagt ctgaagtaat tattgtaatc tgcaaaatgc actcccccga tagcaggttg
     9181 agtgttcatg gtataggtta cgtagtcgcg gctatttgca taattagacc tccctttaaa
     9241 aaagtaccat ggagggtagc tgataacatt acatatctca tttgcggttg cactggtaaa
     9301 ccgtttaaca ccatatcgct cattataaaa attggtagat tcaccttcat agggaacgtt
     9361 tatttcatta atagttccgg aacgcgtgta ggtaaacatg caacctttaa ttgatgggaa
     9421 gattttacta aagtttatag gtaatgacag ggtgttcata ataaatccat aggtggaatg
     9481 cgagcgattg aatccataat cataccatat ttctgatggc aaaaaatctt taaattgatt
     9541 ctcaaaatat gaaaattcat aagaaacgga tgggttaatg aattgataac gctggagccc
     9601 aatggtaagt gaactttttt ttaatcgctc tgtaaaacga atagtatcat catagtaaaa
     9661 aggcggatga aaatttgagt taatctcgtg atatattaca ggattgatat ctgaaacagt
     9721 gcctctatag gtaacaaatt cttctgatag tacctggata ccgggagaaa tatacaaatg
     9781 gggaaattga tactgtatgg atacattgca tgattggcgt tgtttttttt gattgctaaa
     9841 gccgttgaat atagattcac tgttttcctt ataagtatca tccttaaacg atgtatcaag
     9901 caacaaccta tatgatagat caccccccaa tatattgttc agggtttgtt gcaatccaat
     9961 gatagcctga tgtgttattt tattactact gatatcatat gaattggctg attgtttata
    10021 ttcttgctgg ttatcaaatt gttcgtaatt atacatcatg tcaatctgtg gaatcctttt
    10081 atcaggagga atcagggttg agatgaaatg tagctggttt attgatgttg tacctcgtct
    10141 tgttggtata acattttcat tcaggctatc agtttgtgtt tgctcctgca ggtaatctat
    10201 agaggtgtgc cagtagggaa gtatgttaca cgaaaaaaca gcttggctat aatcttccga
    10261 aatgtcatta tatggctgtc caatggtata gaaatcattg ccatgtcctt tttttacata
    10321 ttcaatcatg atatcagaaa gtaccggggt cccttcctgg gttgtaatta aaggctttgt
    10381 tatctttatt gttccttcat accagtgcgc tgtatcttct atggtgatag ggtcacttgc
    10441 gtagatatca tcaagccaga attcaccact ggtattggta ttatgtactg ttacactgat
    10501 agcaataatc ctttttaaat caggggatcc ttcctttttt atgaattgtg cattggttat
    10561 ttcaccctgg agcgataaaa caatatcttc ccatgcatga acaaattgcg gggtataagc
    10621 atactctata taatcattat ccgaggaaag taacttgaag tgaatactat caccactggt
    10681 ataatttcta aagttactcc agcagtgaat atatttataa aatcgaatat ccattggttt
    10741 gtaaaatttt cggattaaag tggttgagtt tatgctattg tatgaaactt ttaaggcgga
    10801 ttctattgtt ttttgtatct ctttctttgt tttatcgcca tacagactgg tgtatacatt
    10861 gtttttttgg agtgcaaatg attctgcggc atattcgctg tcattgtatg aatcaatcag
    10921 ggtaatggaa gctttgttag tatcgccggg agttgtgcca tcgatctcag taatctgcca
    10981 gtgcatggtg acaaagcgga cggaatcaat aaggatggtc ccggtactgg cagtatcatg
    11041 gttcaagagt aaacgtattg agtatatctt ttttaaaata tcctcaaatg cttcagcaga
    11101 tgaggtatat tgagaagggt tacttttgtt aatataaatc cttatcttct gccatgttgt
    11161 gttagagcag tcaattgaga ttggagtcaa taaaccaggg atacgtatat actgctctgc
    11221 agtatcaagc ataccattgt tgttgagatc ttcggtattc aacacgccgt cccctatagt
    11281 tattgacgat agtcctggcc ctgagccaac cactgttggg aaagtattat taaatatata
    11341 gccaacatct tcagaggtgc ttttgtttgg atcatagtct aaatagccat ttctattggt
    11401 atcctcagtg tcaaaaatac catcgccgtc agaatcttcg tttattgttc ctatgtccag
    11461 atataggtta acggttcctg tgccgccagt acttctgtac catatctcac aatattccag
    11521 attggaaaga tccgctggta ccacagctaa tttttgtgtt acaattgaaa tatagtcacc
    11581 tgaactgaaa tcgaagttta atgccagtga acgctgggaa ttttcggatt gtatggaatc
    11641 tgcaatgtga ccggttgcca cattatatgg tcctggtttt ttagagtagg gaatgtttat
    11701 tggtgtaaag tgcaatccat acagtgtttc ggggttgttg atatcacggt aaaacttata
    11761 atagatacgc gcacgcatgt tttgtgaaat actgtcgggc agtgaaccta agatccaatc
    11821 cttttctgac atggatagtg taagcccgga aaaaattgat tcaaaatcat ctatcaaacc
    11881 tttgccaaat gtattgatac tgtggtaact ttttgcatat tcaacgtatc ctctaaatga
    11941 aactggaata gttacattac tattgctcat ttttgtagca aagcttgaga tgtcgtgttg
    12001 cgttaaatca atagtgctat cggcttcaac aacagtagtt tgcgtgggtt catttcctat
    12061 aacgggaatg gttgaagcac ttccctgcgt agtataaaga attgttccgc caacactaat
    12121 attcttgttt aaagcatagt cagctcggac acctcccata aattcttgag actgtccttc
    12181 aaatgggagg tattggtagc ttatctcaat gtctgtgtct tgtgtaataa caggattgcc
    12241 tggattggta aattggaaaa atccggatgt tggatccact gaatagagac tttcagctaa
    12301 ttgtacacca ttaatcttga cacggacagt atcggggata atattgatat gttttagctg
    12361 aaaaccccgt gattgactgt atccagagat gccaattttg aattgtgata ccgtataagc
    12421 agtagaactt tgtgtttctg aataaattac cttttgtgac tgtattgata atagttgctt
    12481 aaaaggttca cgaagcttaa attgtataat tcctgtattg taatcaataa taatcccacc
    12541 caacctttca acatctgtct ttgacatggg aaaaagatta ttaaagaagg ataattttgt
    12601 atcgtcgctc ctcagttgct gtaatccagt agcatacatg cctctgattt catagatgtc
    12661 atagtttagc atcccatcgt tgttactatc ctcaccacta tcaagtactc cgttatagtt
    12721 gaggtcttca tggagtgatg aagcatattt taaaaataca caaatttttc cggagaattg
    12781 tggatggctt gtaatggctg aagggtctga tgtctgggtt gttccgtttc tggtgtacac
    12841 tgcaaaaagt tttgcttgtg attttagatt cttgaggagg gtgattgtac cggtggcaaa
    12901 attaatggtg aaatcactac cctgctgtag ctttttaaaa tatcccaata ctattgatgt
    12961 atcgttccat ttgtaaattg ccatcaatcc ggttttattt tcagatgatg cctcatcagc
    13021 ataaagctca aaaccggaac tatctatccc aacactatac ggagaatagg ttgccggtga
    13081 cggaggattt gaattccatg ttatagtaga ataaggattg gctggagggg catctgcatt
    13141 atcaaatcgc ttgaagggtt ctaattgata gtatgttccc ttggtgtatt gatattcctg
    13201 caataagaca cttgtattgg agtaattgcc cttaaatcgt tcaatctcac ttaccccttt
    13261 ggaaacagaa ccaaatgcct tgagtttaag gtcacctttt tttatggtaa aatcaatgcc
    13321 gacacctttt gaagttgtgt catcatatac tgcgtattta gagttgttaa ccttaatatt
    13381 gatatcgcca gcattaattt cacgtattac ttcatcatca ttcactgcct tgtattgtaa
    13441 aacataggta ttattttgct gttgactgtc gtggtcaata tacaatgtaa ggcgcttgcc
    13501 agctttgccc tctatatgga gctgtgaatt ttgctctata gttaaaccag attgaatgag
    13561 cgctgatgtt ggtttgtctt catcaaagcg tttatactta tcattggtaa aataagattt
    13621 gccatagtta acattaatat tcattgaacc atatgaaaga atatcatatg gctcttcttc
    13681 atatatttga ttgcttataa tggaaaacga gtatcgaggt tcaatgatat atttagtcca
    13741 atcccctgag ttatattcaa agggtgatag ttcttgttga ggttgtaaaa tcaaaacatc
    13801 gtcaagcccc ctggcagagg gttgtactgt ttgttcttta tgagtatcaa tgtagttgtt
    13861 aatgcgttca tgaaaggtgg atgatagttg ttgggaaata acaatagctg ttatggaggt
    13921 aaggatagca ccagcaataa tgatattgga taatgatttc atgtgttaga gtaataacca
    13981 tgtttgcaac aggaaaatgg acaattacca catcttgctc atatacggat aaggattgat
    14041 agcaacacct cctatcctga tttcataatg gcagtgactg cctgtcgcag aaccagtttg
    14101 cccaacataa gcaataatct gccctttttt aatagattgc ccttgtgaca cgataatctt
    14161 ggagcaatgc ccatatattg tttcataacc atatttatgt cgtattctga ccatataacc
    14221 gtatccacca ccccacgatg cggatatcac aatgccaggg gcagtagcgc gtatctcagt
    14281 gccttgagga gcagctatat caatgcccga atgaaaatct ctatcaaaac caaaagggga
    14341 acgcctccaa ccaaacaacg aggttatatg gcctggattg gggatgatag atggtgtatc
    14401 atagatgacc ttattacgaa catcgataaa attcttaatt gttttgaccg tgttagtagt
    14461 acagagtaaa tccttctgca tgctgcgcaa tacctgtata tctgaaggta aaattgaagc
    14521 atattgacgc atcgatgggt cttcattgtt cactaccgaa ctttcccata tggaagttga
    14581 attattgtta ttggtagcca gatcgtaaag gtcttctata tcaggtttta ttttatcaag
    14641 ttcttgacct aattcattgg ttaattgttc aaaacgtatt atctgggaac gaatatcctt
    14701 gtaattggaa agcaatcgct cttcttctct tttaactgcg gtatttttaa ttattgcata
    14761 ggaggatgta acaagaacta tgaccagtaa agtagtgaaa aaaagtatgg taaaacgcga
    14821 tatctgaaag ttaaaaatct tttgctcgtt atgtgggata aacatgatgg tcattttttc
    14881 atgaccatgt tcaagtacct tatcccattt ttttaaccat tgttttttct ttaaataata
    14941 tttttccttc agctttcgct caatattgct tttaaagatt tttattcgta ctttcatcaa
    15001 tactatttct cactatagtt ataaataata attattcaat ttgttatgat acatcaattc
    15061 atatgatttt aacaaaagat aaatatatat aataaattgc aactataata aaatatgcta
    15121 ttattatata tatcgtatga tagatataat aattatgcaa gttttttttg gtagtatttt
    15181 attaaatcgt tgaagtgaat tatatggaat gtaaacgatg tggaaactgc tgcaatgatg
    15241 tacggcttgc agagtcacct gaagttttac aaagggcgta taaatactgg ttgcaattat
    15301 ccatgataaa ccctaatttt tctgaaattt accttatcta tcccatgtta acctttaaat
    15361 atcaaaatct ttctgaagat ttgccatatc attattcctg caaacatttt acatttgact
    15421 ctcatggatt ggggctatgc agcatttatg atataaggcc cagaatgtgc aaggatttcc
    15481 cctattatga tggtattgca ttagcacctg aagaaaagtt aagtccttat gaagggtgtg
    15541 gatataatga ataggtaaat gtatgcccca atttgtagaa tatatagctt tttttgcagg
    15601 tggtatttcg cttttagttg cagttggtca attgtgtgta cctcatcgca ggcttgaaaa
    15661 cctgaatctt tttgggttat tcctggtttt aggtctttta ttgtttcaat atggatgtat
    15721 attgaatgga tttattttta aaaaaccgta tcttttttat ttccatttta catttttata
    15781 ttgtattggt ccacttcttt attttgccta ttaccttgtt atacggattg aagattcact
    15841 tccaaaaata aatttattac tgctgctccc ttctgttatt tattgcataa tagatatatt
    15901 ctttatacgt tctgattaca atttgcagat agccattatt aatagtttat tctttgataa
    15961 ttttggtatc tatcactggt taagaattgc agttattgca gcacttcttc tggatagttt
    16021 atacatcggt tttttattgc accgtttatt ccaattctgg aaattgcatg agttgacttc
    16081 gataatggtg gtgacattaa tattcagcat aagttcgttt gccgggttga tgattctatt
    16141 agcaagttat tgtatgcgaa gccttattgg gataaaggta agtgtcatta taatggctat
    16201 cttgcttatt gcagcgtatc tggttggaca gcataaacct gagtttttac aacttcttat
    16261 acgtgaagcc cgcaggaaaa gatatgaacg caccttgtta aaaggatgtg aagtggaaag
    16321 cctttgtgaa aaactaaaac aattaatgga cgaagaacgc ctgtatttag atgaagatgt
    16381 aacgctgaaa gaagtagcca atgaacttaa tattaccgct caccagcttt cagaattgct
    16441 gaatgtaaaa ctacagatga attttaacac atttatcaat caatatcgta ttaaagaagc
    16501 aaaaaagatg cttattgagg aatctgagag atcggtgttg tcaatagcat atgctgttgg
    16561 gtttaattcc aaatcatctt tttatgaggc attttcacga tttactggca aaaccccagt
    16621 gcaatataaa agagaaaaaa agaattgcac atatgctatc aatgaataat aagaattatg
    16681 aagagtctta atttataaat caagctaaag gaaatgataa aaaaagcttg tattaagaat
    16741 aaatagtgtg tatggtttac ataatggtag gggtatacaa attaaaatta atcagggtaa
    16801 tattgtgtcg aaagaatttc aatacaaatt gtgggcaata gcattttact catttgtagc
    16861 agcttattca tatcttgtac ttacagctgg ttcgggcaat gatatacttt tctggtcata
    16921 tgaagaattt atatttggta ttagtataag tattattctg gctttactaa ctttttggtt
    16981 aatacctatt aaagttacag cagcacttct taatccattg ctctatattg gattaatctt
    17041 atattttgtt ggcccgtttt ttctatcgtt actcattgcc aatgttgaag taatgtacag
    17101 gattataacc ggaaagattc agccggctat tgttaaagtt gatcctgaaa tggatgatga
    17161 actgggcatc tatctgcttg ctaattccat aacattatca cctggaacat taacagcagg
    17221 ttatgaccct gataaaaaga agttctttat acattgtttg tattgggtca aggataaagg
    17281 ttataaagca acaccaaaag atgtatcggg gttccagcat tattttttaa agaaattatt
    17341 taaataatat ggatcaacat atgtatgatt ttgatttaat ttttttaatc tcagggcttt
    17401 tattgattat ctatgtttgt atcatagtaa tacgcattgc attcagtcac aatgtgccag
    17461 aacgaattgt agcacttgac acaataaata cactaataat tgtcatcatg attgtgctgg
    17521 gcgctgcaca aaagaaggca ctgtatattg atatcggcat tgtttatggg attatttcat
    17581 ttattggtac attatatatt gcgcgatacc tcatagatga gaagcggtaa atatggatac
    17641 tataatttgt atatttttag taattggctt gctggttaat ggcgtaagca ttattggact
    17701 tatgcgcttc cctgacatct atacacgatt acatgctgcc acaaaaacaa cgacttttgg
    17761 cagtattttt gtagtgttag ctgtaggagt taaaaatata tggtattatg atgctacaag
    17821 cataacaata atcattcaca gtattgtagc tttacttgtt attattttta ctaatccaat
    17881 cagtgctcat gcaattgcgc gggcttcttt gttatcaggg ataaaaccat ttggtgttgt
    17941 tataaatgaa tatgattata aatctcagat aaaaacagtt gatgatctgg aaatggataa
    18001 aattagtgaa gggattccag atgaggaggg tactttatga tctattattt tttcctggtg
    18061 atttcgattg ctcttgttat tacagcattg ttgagcatat attttcggga cttactggca
    18121 gcgatagtga gctattcggt attttccctg gttttgacag tactatattt tcttttagat
    18181 gcaccggatg ttgccattgc agaagctgga attggtgctg ctttaacaac atgcattttt
    18241 gttatagcaa tacgaatgac acggagaaga gaggaatgag gctgatcagt gttgtagcga
    18301 tagttgttat atttggattt atctttgtgg ggatacttca cctgcatcca ataggtgaac
    18361 cgataggtaa tcccagaatt ttatatgaaa ggcaaccatt gggtatagat actcaaggtg
    18421 ttactatcat ggatgattat tttttacgca atggtcaggt cgaaacaggg tcaaataaca
    18481 tagttgccag tattgttttt gattatagag gatttgatac attaggggaa gccacagtgc
    18541 tttttcttgt ggtaagtagc atttctatgt tattttttac ttttttgaaa agtaaaagta
    18601 ttatatcggc gggagtttct aaagcgggac aattcccaaa gatagaatcg agaattataa
    18661 cgtttggtac tttcttaatg tatatcatga tcttaagttt tgggatttat ctggtagttc
    18721 atggacatat ttctccaggc ggtggatttc agggaggttc tgttatggct tcagctacag
    18781 cacttttgtt aatcagtttt tatataaccc gtcacatgca aagaaccaga aaaatatttt
    18841 cattgtttga atcactgggg ctgactatat tcattggaat gggttttgca gggattggga
    18901 tttccttttt gtataatttt ttggctcatg gtaccacagt atttggtaaa ataatcccct
    18961 ttggccccaa tcctggctat ttgatttcag ctggtatttt gccatggcta tctcttgctg
    19021 ttggcataga ggtttttttt ggattaagcc ttattctggt tacgttattc catgcatcag
    19081 gtgtgaaaaa taattcatta taaggtgtta ttatgtccgc tatcatactt tatataactt
    19141 ttgttttatt gtttactatt ggattatttg cattaatatt taagcgaaat ctcatcaaga
    19201 ttgttattgc acttaatatt cttgattcag gaatgaatct tttttttatt actctgggat
    19261 accggcataa tgcaacatct ccaattttta cattactccc atgggatact cacataacta
    19321 tggtcttgcc caccccgcag gcgttgattt taacaaacat cgttattgga tttgctacta
    19381 cggcattgat gctgggcgta gcaatattaa cctatagaaa taataaaaca ctggatacgc
    19441 gtaaacttcg tggagttgaa gaattatgat aaagcatgtt ccagtattat tggttacgat
    19501 tcctcttgtt gttgctttta tgctccctat tataggtatg gttaatcgta ctatacgcac
    19561 tataacagca ggcgtggcta ttgttgttat catggtgcta tcatatttgc tgatacagga
    19621 tgtaatgatt tataaaaaaa ttattcatta tatggtaggt tctcctgatc caaatgcttt
    19681 attggaacat ggattaacat tccctatacg tatagcattg aaaatagatc aattttcgtt
    19741 attatttatt attatggtta caacattatc atttattttt tatttatttt catcgcagta
    19801 tattgaagaa aatgaaagga aaaattattt cactgtttta tatgtctgta tggttgctgg
    19861 tatgatgggt atgcttatta cgaatgattt gtttacattt tttgtttttt tagagatact
    19921 atcgatatca tcgtctgcat tggtttcgtt cagaacagaa cggaaacacc caccctatgc
    19981 cggttataaa tatatgtttg tgagctcaat cgcaacgagc ttctttttac tgggtattgc
    20041 attcctttac gcacagtatg gctcgtttaa tattgactat ctcaaaaaag taatgaatgg
    20101 aaccgtactg gacaaaattg ctattgtgat gctcatttta ccattagcta tgaagtgtgg
    20161 tgttgtcccc atgcatatgt ggatacccga tacttactct gaagctcctg ccccaataag
    20221 ttccatggta aaattaggta gtttgatttg tttatatggg cttttcagga ttgcatttac
    20281 ttttggacta agtcgtgcac aggtaagtta tgaactgggg atgctattta tactatttgg
    20341 tgtgttgtca atgtttgtag gggtaactca ggcacttata cagaaagatg ttaaacgact
    20401 tatggctttt catgctgttt ctcaggttgg ctatatgtta ttgggagttg gtgtagggct
    20461 ttcggttatt aaatcgggtg atgcttcatt tgctatttat ggaaaaatag ccataatagg
    20521 tggacttttt catgtggtca acaatgccct ctataaagga ttgttatttt taacatctgg
    20581 tataatcgtt agacactata aaacgcgcga cttgaatata atgcatggat tggctcatta
    20641 tgataagttt actgcactgg tttttatgat tgcagcatta gcaatttcag gaataccacc
    20701 attgaatggt tttgtttcca agcttatcat ctatgaatct gtatttcact ataatccgtt
    20761 gttaacattg atagctattt ttgtttcagt tttaacattg gcttcattta tgaaggtttt
    20821 ttacagtgct tttctgggaa tgcctcctga aaggaggccg gttgaacaac caatattggt
    20881 taaatgcagt atgattattt tgagtattgt tataatagtc attggcttgt ttccccaggg
    20941 tattataaaa ttattgattg agcctgctgt aaatgtatta tctacttttt aaatggagtt
    21001 atgaatgcat gaatttgaat tagaatatct taactggatg cctttattat gggtttgtat
    21061 ttttttaata attatggttt tgtttataat tattggaaaa atatttttta gaaatgattt
    21121 tgatgagatg tcagaacagg gacttccatt ttattcagga accagtgata aaatgctgca
    21181 attaatcact gcaagtaatc tatactgggg atttaagcat accttgcaat catattttga
    21241 tggtttaaat agattgttca tcagtgatat tggtgatttt attcaatggt ttattattat
    21301 cgttgctata atgttattga taataacagg ggcacttatg tgaaaaagat taattacaaa
    21361 catttgaaac gttcattatg ggcatatcat atgaataccg gttcatgcaa cgcatgtgat
    21421 attgaaatac ttgctatgtt aatgccaagg tatgatgctg aaaggtttgg cattaaatta
    21481 gtaggtagtc cccgacatgc ggatatatta ttagtaactg gaacggtaac ggagaaaatt
    21541 aaagataaaa tactgcaggt ataccagcag gttcctaaac caaagctggt catgatcata
    21601 ggcagttgtg gtgctaccaa aggagtattt caggaatcat atgctatggc tggcccattt
    21661 gatacattgc tacctgtaga tgtttatgtt cctggttgtc cgccacgtcc tgatgcaata
    21721 atctttgggg ttgcaaaagc ctgggaaaag ttagaagtgt tgtaaaaata gttcatgtgg
    21781 taaatggtat ggatgttcaa tcaattgtga cgattattca ggaaaaatta gggaatcacc
    21841 tccaggcttc aaggttgaag gtgaaaaact accatggtgg aattatgcga ttgcacctat
    21901 ggatcagtat agatcgagag gcattccatt ttacggtaaa tactctaaaa agtttaggga
    21961 ggcttcacct atctacagca atggcttata aggaatatga agatcgtatt gaacttatct
    22021 atccatttgc cctgaaaatg gatgatactc actattctga attaatgatt attgcatctg
    22081 tagatttacc taataatgat ttaacaatca gaactattac cgatattgtc ccgggagctc
    22141 tttttatgga aagggaagtt atggaaatgc tagggatagt aattgaagat atccctgata
    22201 gcaggaggct atttactcct tcatccttac aggatggata tttccctctt cgtaatggca
    22261 aaaacaagga agatagcaaa tgaaatctga tagagtatat cctttaaata taggaccgat
    22321 acatcctgca tttaaggaac caataaaatt tacattcagg atagaaggag agcaggttac
    22381 tggtgttgat atagattttg gttatacaca tcgagggatc gaaaaaatag ctcttgaaag
    22441 aaattacatt cagattatct atttactgga gcgagtatgt ggtatatgtt cattttcgca
    22501 tccactggca tattgtcttg ctatagaaga tgttgctgaa atacaggttc ctgatagagc
    22561 tcgatatatc cgcaccataa taggtgagat ggagcgatta cattctcatt tgctgtggac
    22621 aggtgttgca gctcatgaaa ttggttttga tacgcttttt atgtatacat ggaatattcg
    22681 tgaaaaggta ttggattgtc ttgaagaact tactggcaac cgtataaatt atgggatgct
    22741 caccataggt ggtgtcagga gggatgtaac accagagaat tcagaaacta ttacacgcat
    22801 gttagaatat tatatggaat tgtatgatcg tgcttcggaa atacttctgg atgatttaac
    22861 attacgtgca cgaactgaag gtattgggat cttatcccgt gagcaggcag aaatgttttg
    22921 tgcagttggc cctactgcaa gaggttcagg attactgaaa gatgtccgta tagattatcc
    22981 tgttaatgct tactatgata tcccatggtt aaatcctata tcaccacgtg atcttggcaa
    23041 agaacctatt ggtgatgtgt atgatagaat ggcagtacga gtgctggaga ttaaacaatc
    23101 attaaatata atacagtttt gcatggaaaa cttaccagaa ggagatattc attcaggtga
    23161 tggagcagtt aaaattatca acacgttgaa gaaacttgaa ggtgagggta tagggcggta
    23221 tgaagcccca cgaggtgagg tatgccatta tgttatactg gataaccagg agcaccccat
    23281 acagttaaaa gtcaaagcgc ctacctattc aaatggattt acatggatac ctatgctgac
    23341 aaatattgag atagctgata taccaatagt tattgcttca atagatccat gcgtggcatg
    23401 tgcagataga atgactttta ttagaaaagg tggtgatgaa gaaaggtaca actgggaata
    23461 ccttcatcaa aaaagcatta agaaatacaa gaggtcagta aatcaatgat tatggtactt
    23521 gccatagctc tcatcattac gatagttgga ttaatattag gacttttgtt gaaggggata
    23581 gatcggaaaa tagcagcata tttccagtca agaattgggc cacccctttt acagccagtt
    23641 tatgatatca ttaaattggt gtataaacaa actattatac cggtaacagc ggtgaggtgg
    23701 ttgttttcat ctgctcctgt aatagctctg gcatcaagtg tattgctact attatatatt
    23761 tcgatgccat atatattcta catcactggc attgatacta ctttgcaggg tgatttaatt
    23821 gtagtattgt attgtattct tatcccttct atagcaatag ttactggtgc tttttcgtct
    23881 ggttcaccgt atgctgcgat tggtgctcag cgtgaaatgg taatacttat gagtatagaa
    23941 ttaccaatag ctgtagtaat aattagtgtt gcatggcatg tagcagatag tatggctgat
    24001 ccttttgcta tgtcatcatt atttgaatat ccactatggc ataatgctgg tgcatttggg
    24061 gtattgggtg gcatattatt gttttttgct atggagcttg ttttgccagc tgaaatgtcc
    24121 agagtgcctt ttgatcaggc cgaggctgaa actgaacttg cagaaggtgt ttcagttgaa
    24181 tattcgggta agctactggc atttatacta ttaactgatg cttttaaagc attagcactt
    24241 tcaatgcttg ttataatact ttttttccca tatacgctgt cccaattgtt tggtatatct
    24301 tttcagatgt atggacatag agtggcccct tttgttgaaa tagcattttt attatttaag
    24361 attatagtga tttacagttt tgggataacc tttatccgtg ttattatggc acgtttaaaa
    24421 atatcacagg ttgcgcgttt ttttcttttt gcaataacag caatcagtat tattgggtta
    24481 atacttatta tgcttgatcc aaatatgagg attatatagt aggtgtgcca tgttaggtat
    24541 ggtatataaa gtcattgaac agatcttttc aaaaaaatcc accaattgtt ttcctacgaa
    24601 atatattccc aataatacta aacaatacct taaccaggga ataatacatc caccaattgt
    24661 cgttaaagaa ggattcagag gcaggctgga atacgatttt cagagttgta caggctgtgg
    24721 tttatgcagt aaagtatgtc cagctaatgc aattgaatta tattctgctg aagttaacaa
    24781 taagaagaca aaaaggatag tcatctattt aacgcgatgt accttttgta aggagtgtgt
    24841 tgatatttgc cctagaaatt ctataaccat gacaaaggca ttctgcatgg cagattataa
    24901 aaaatatggt aattcactgg tggttgggtc ggaataccga cagcaatgtg agataaagga
    24961 tcatactgat gttgtgcaaa aaaagacaaa ttgaaatata taaaaagcta cataatggtg
    25021 atgagatata ttgtttcaga ccatcgggtg ggtgtggaaa aacttaaagt aaatagtcat
    25081 catcccaatg gctcaatgaa tgaaaaatat ttgtctgggc tcctttgatt gttttattaa
    25141 gattttttaa aagttctttc atttgatatc gttttgtgca atctttgtaa ggacattcat
    25201 tttcaatagg tgatagtgga agggctttta tataaccttt gatctgtgct tcggttaaaa
    25261 aagccagagg cctgatgatt tccaatgcac cgttgaacat ttttaatttt ggtggcatgg
    25321 tcgatagttt tccatgataa agcatattta aaaaaaaggt tgttattata tcatcttgat
    25381 tatgcccaaa cgctatcttt ttgcagttga gctgtgctgc tgtttcaaaa agagcttttc
    25441 tccggtacca tgaacaaaca aagcacgggg atgacccttt gatagaaaga tcaagtgtaa
    25501 tgtctttaat tatcaggggt atattatata ttgaacagta tgattgaaga atatttattg
    25561 gtttattatt aaaatttgtc acatttacat gacagcaata tagagtataa tgaataggta
    25621 aatacgatag ccggcgatat aaggcatcca tgcaaactaa tgaatcttta ccgcctgata
    25681 atgctatgca aatagtatcg cctgcttcta tcattgtata cgtgttgatt gctttgccaa
    25741 cagttttgac atatttggta atttgctttt ctttcatttt gatgtgcata ataaagcctt
    25801 gacgtaaata tagttatatg gttattagta gtatgagtag attgcattgt caatagtaat
    25861 tgtgtgtaat attaattgtt attgaggtta tgaaaatggc gcgctgtatg atagttgatg
    25921 attcaagttt tatgcgtcga attattcatc aaacattaga ggaggcaggt catcaggttg
    25981 ttgcggaggt tgataatggt ttagatgcat tgtcaaagta taaagaagtg aaacccgatg
    26041 ttgttaccat ggatattacc atgtggggga aggatggatt agaagctatt tcagaaatta
    26101 ccagtgatta tcctgatgct aaaatcatca taatatcagc attgaatgaa agaactttaa
    26161 aaataaatga gaaagatatt aatgctaggc tatttttatc aaaacctttt gaaaagcagg
    26221 aactattaga tgcaatagaa aaagtcttaa acaatggatg aacaactttt acaattatat
    26281 aaaaaaaatg ctattgctga taatgcatat atgattgctg atggtacagt atttttattt
    26341 gtcacagaag ctgataaata tgttttgcag gggaagaatg taattatagg tgccacggaa
    26401 ccagtattaa acagcaaaac gtatgaaaat atacacagac ttgaaacagc cttagctgtc
    26461 aaaggtacaa caataaaaaa aattcctgct aatactatta tacagagttt aaataatact
    26521 gcttttacca tgaatctggc tatagttgaa gctaaagttg ttgtattgac aaatgacatt
    26581 ttgaagaaaa atatgcaaat cgattctcaa ctggtgaaag aacggaatat atgtattgaa
    26641 tattacacta ttgtgcattc actgcaactg gaatatgaaa aaagaaaact gccatggtta
    26701 aaagatctaa ttgataaatt taaaaccagc ataacattta aaaaaggtga agcattttcg
    26761 cgggcaaacg aatcattacg aattgatact cccagggaac tatctgcaaa tatgatagag
    26821 tttcctcctg gttcgctatt atgtgaagaa ggttcggtgg gtgatgcaat gtatatatta
    26881 gagtcagggc aattagaggt ttatatacaa aattcaaaag tggcaacgat atctgaaaaa
    26941 agtactgtta tcggtgagat tgcaatgctg ctttcaatgc acagaacagc cacattgaaa
    27001 gcacaaaata cagttgttgt tactaaaata acacgggaag atttaagaag gtcaacttct
    27061 ccagagttga cgcaagcatt gctggtttcg ttagctaaaa aacatcatgc aaatgtgttg
    27121 catattatgg atataaatga aaaaaaagct cttgcttatg cagactcccc cagacaagag
    27181 ctcatcaaaa ctgaagcata tagatatcaa aatgagctat cacaacttaa atatacactg
    27241 gaaaaattaa ttcagagcaa acaagctgat tatctggaat atattttaaa caaataaaac
    27301 ttactgttca acctctgtta atacttcgcc ctttttaagg gatttcagcc agtttaattc
    27361 agctttgaga tgattttcta gataggtata aacgtgtaac tgagagtttg gtgaattatt
    27421 ttttaattca gtatcaattt tttcttttaa tgcattcatc ttatctttta tatgctcaac
    27481 acgatcttct atgaattgtt ctttttgttc aggagaaaga gaattcagga acatcaatac
    27541 gatatcaata tcaaaatgta tgaaattatg gtcaaaatat tgtttcagca tctttttata
    27601 gtattttcgt ccgtcaggca gtatttgata tatgtaccgc tcaggattac ctccttcctt
    27661 ttcggtacct atttttttta cccagccatt atctaatgct ttcttaattg cataataaat
    27721 tgaaccaaat ttaatatcta cataatcttc aaggcgttca actatacgct ttttaatttc
    27781 atagccataa agattttctt ccatcaataa tcctaatatg gttatttcaa ttttcatgat
    27841 gcacctcgtt tgaataaatt tttattataa aatttataat ataaaaaata cttataaaaa
    27901 tcaatgataa tattgtatgt tcataaaaaa aatttacaaa tagtttaaat tagattatat
    27961 aaataagact taatatttca ttatgtttaa tatagtttaa ttttactatt attcaggtgt
    28021 ttaagcattt atcattcgat ttctttcaat aaagaactgt aatgaataac aattgtatat
    28081 caaattttaa ctcctcggaa aatatataat atggactcct gtcattccgg gctttaaccg
    28141 gaatcgcaat atgtaactgt aaattccgaa atgagttcag gatgactgct ggcaactatt
    28201 tttttcattc cctctcagtg tcagcatgct gaccatcatg aactctcttt aaataacaag
    28261 caaaaacaag caaaatttac aacactaatc atgcaatact gtagaacttc caatcatgat
    28321 aaatcaatat ccatgattat tgcgataatt gtttcaatga taatcggatt attgtctcta
    28381 aatcacaatc aggattgctt gtgataattg aagaaatagt agacattgca agtttttcat
    28441 caaagcctaa tgacaccaat gcatcgatag cgtcctgtct atcgggggat gttgtagcag
    28501 gatgctgaac agtggcttct aatttccgaa gtttgcgttt tagttcaaag attatttttt
    28561 cgcttttact ttttccaatt cctggtatag cggtaaaggc aagaatattt tcagctttca
    28621 cggcgttggt aatatagtga ggcgacatag atgataaaat tgatattgca atggatgggc
    28681 ctaccccggg aattgatgta aggattgcaa acatctgctt ttcttcttct gaactaaagc
    28741 catacagggc aacggagttt tctctgacta ccatagcaat ataaagctcg atagtagcta
    28801 cagaacttat tttttcatag gtagataggg ggatggtaac ctcgtaccct accccattaa
    28861 catcaacgat aacttttgtt ggtttaatag atattttttt gcctttaaga aatgcaatca
    28921 tataaaactc aaaattgaaa taaatttata ctttttggtg taataatgat ctttctggag
    28981 ttgccagaca atgacatagt gctattgcaa ttgcatcaaa tgcatcatca taattatgta
    29041 ttgtatcgca ttttaaaatt tttctcagca tggtcgatag ttgctctttt gaagatcttc
    29101 catatccggt aatagattgt tttatctgca ttggtgtata ttcagaataa ggtatagcat
    29161 gctgagttag tattaactta caaacaccta tgacctgcac taccgataga gcagttttag
    29221 tatttttact gaaaaacaat ttttccaatc cacacgattg aggctgatat aaagcaatta
    29281 aatcacctac actttgatat atagtaaaaa gtctttcacc ttcgtcacca ggagttgttt
    29341 caataacacc acagtcaata taggttaatc tatcggttac tatcgcccaa ccacagcgtc
    29401 cataaccggg gtcaattccc attatagttg ccacatcgat tagctcatac tattaataat
    29461 atcatccggg atatcaaaat tggcgtatac atcctgtacg tcatcatgat cttcaagagc
    29521 ttcgatcaat cgtaaacatt gtgatgcttt ttgcccttcc agattgatgg ttgttttggg
    29581 aatataggtt atttcactat ttaatgttgg cagattgtga gaacgtatga tttctaatat
    29641 gtcattaaat ttatcaggag aggtatagac aacaatggta gtatcttcag tttttatatc
    29701 ctcaacatca taatcaagga ggatttccat aaccttatcc tcatttgttg catttgcatc
    29761 aatgttaatg acgccttttc taataaaaag gaatgaaaca catccagatt cgcctaaatt
    29821 tccgccattt tttgaaaata tagtgcgtat ctctggcgtt gttctatttt tattgtcagt
    29881 catgcagtca accatgatgg caaccccacc ggggccgtac ccttcatagc gtacttcttc
    29941 gtatgttgaa ccatcaagag ctcccgtccc tttttttatt gcccgttcaa tgttttccat
    30001 tggcatattg ttttcacgcg cttttaaaac agcagtccgt aaacgaggat tgctattgag
    30061 gtcatcacca ccgacgcgag cggcaacggt gatttcccgt ataaatttgg taaatatttt
    30121 tccctttttt gcatcctgtg cagcttttct gtgcttaata ttagcccatt tagagtgccc
    30181 tgacataatg aatgctcctc acaatacaat ttgtaaacat gaagtaaaaa ctaaagtaat
    30241 gacgaatgtt agaaatgtca ttgattttgt cattaataaa ataatacatc ttttcaataa
    30301 aaaaattttt attaaaatcc tctggaaatt tttagctgta atttttcaat ttttttgctg
    30361 gattctctta ttttaattcc atactcaatg gttttgttat accattcaat tgcttgcgca
    30421 aattgcatca tattctcatg ggcgacagca attttaaacg atagtaatcc tgcattcaat
    30481 ttgctgtatt catgttcaag ctgccgtaat tcaagttcat tgtttcctaa ttcatccatt
    30541 agcgtttgat attgggggta taatgcaata tctttattag caagcaatgt tgatttaatt
    30601 acgtttattt tatcattgat tgtattaata ttggcttttt tttcatctat tatttttttc
    30661 atattttccg atatggctat tgactgtaga tagtattcta aaccattttt gtaattaaga
    30721 tccatacatt caatatcacc taacattgta taagatagtc cttttaatac ccaatcattt
    30781 tcattgaaaa gtgggatagc atttataaat gatttgtatg ctaacttcat atccgaaatt
    30841 tttgtataag caaggtagcc taatgcaaag agaacagcgt aatcattggg tttatgatga
    30901 aggtataatg tatattgttc ggatgctttg atgtaatctc ctattgattc atagcagttt
    30961 gcagcgatca gatagagatt ggggggaaca ctatcacctt ttgatgcaat ataacggttg
    31021 agatgaaata aagctttctt tttattcttg ccagatgaaa gagctaagga tagttctaaa
    31081 gaataatcat aattatctgg tttaatttca tacgcttcct ctaaatagag cacatacgca
    31141 ttaaatttat ttattttttt aaaaatttgt gctattgaat aaaagattgt atgatccttt
    31201 tctccaattg ttgtaaagcg taatgcctga aaataatgct gtaatgcttc atgatatttg
    31261 tagatgtttt gataacaaag tgctgcatca gcatgtaatg catagagggt atcgttattt
    31321 tctataaaat atggctgtat ctttctttct gaaaaaatct tttgtaaatc aaccccttct
    31381 tcaatctttt caatggcaac ataggtggtt ggtatctgct tttcaatgga tctttttgaa
    31441 tatatatcgt caagcttgga ttggataatt gtcaaagctt cagagaattt tttttggtgt
    31501 atagcatctt tataattctg tccaaacagg tagctgttca tgaaaaatga taacaataat
    31561 gtaattaaaa ttggtagttt ggtattcata gtggttatta ggtatacttt tattgatatt
    31621 atcggatgag aaaggaagta agttaaaagg aaatgcttat ctacggatac gtatcctggg
    31681 caattgccgt tttttaattt tcagcgcacc ttcaggatta tatttgtttt gtatttcttc
    31741 gatactgtgg ctaatgagca ttcgtactac ctcctcggct ttattgagaa catctattaa
    31801 gataaaattt tcatcgtctg agaaatcatt taacagatgt tcttccatcg ttatgccttt
    31861 atgtgggtag ccaataccaa ttcgtacttt tgcaaatcta tcggatttca aagcctttgt
    31921 catagattct attccagggt gcttgagctt ggacataatg gaatcaacac gaatttcgcc
    31981 cagaggcaaa gacgaatctt ccaggacaca gataatatca cggacattga tacgtaaaaa
    32041 agaagcaata tacagcactg actcaccaat aagggtaaca aaggtttgag gtttcaacaa
    32101 aacgacatcc tcacctgaaa ttttgccacg tccaatgata gattttttct ttttgacacg
    32161 tatgtcaata ttctcgttat tgccaagaat atcgacaacc ttaaagccaa tgttttgcct
    32221 gttattgaag tattcttcgc ctggatttcc aaatcctacg attaatttca attacataca
    32281 cctcattact aaataattac tgttcttcaa cagtttcttg aggttgttcc ccagctggtt
    32341 gtgcaacctc ttcggtggct ttatgtggtg caagtacagc tacaataacc gattcaggtg
    32401 aacttaggat cttaacaccg tttggagcta taatatcctt tacatgtata gattgcccga
    32461 ttgctaaatt tgttacatca atttgaacct tttcaggtaa atctgctggt aggcactcaa
    32521 cttcaatttc gcgctcaatt acttctaaaa tcccaccctg cttaacacca atagaagttc
    32581 caataatttc tataggtgct ttggtagtta tggtctcggt cattgttact ttaaaaaaat
    32641 caaggtgtaa aatttcatcc gttatagggt gtcgttgata atcctttata aaagctttta
    32701 ccggggcact ggaatgatca ttaaattgga ggtcaatgag cagattttct gatatatgtc
    32761 ccttgaagat tttaaagaaa ttcttcttct caaccattac agcctgtgat acgccatggg
    32821 agtatagaac agcagggatg tatccctgtg ctctgagcct atgattggca tttttcccaa
    32881 tttctgatct cgttttaatt ggtaatactt gtgcttccat atcaacctct tccagataat
    32941 ttaatataca ttatagtaaa aatggtataa aattcaataa ttatttacta tgacattatc
    33001 gtataaataa tgaacttacc gattctccat tatgaattct tcgtattgcc tctccaaaaa
    33061 gtggtgccat agatagtatt ttcatattgg gtaatatttt atgttcaggg atatctattg
    33121 tattggttaa tatgatttct ttaaattcag cctttttaag tttttcgata gcattgtcgg
    33181 ataacactgc atgcgtggca gtacaataaa tatcttttgc tccactcttc tttaaggcat
    33241 ttgctgcttg tgctatagaa cctgctgtat caatcatatc atcaatgata atgcagttgt
    33301 gatttttaac gctttcctcg ccaatgatat tcatcacctc cgatttgttt ggttctggtc
    33361 ttcgcttgtc aacgattgct aattcggcac tgatattacg agctaaaaat cgagcacgtt
    33421 cagttccacc tgcatcagga gaaaccactg ttggattttg tatattcaat tcattgacat
    33481 attcaataac aactggagat gcaaagagat tatctacggg gatatcaaaa aaaccctgaa
    33541 tttgatcgga atgtaactcc atggtaagaa cacggttgac acctgcagca tggagcatgt
    33601 tagctataag cttggcagag ataggaacac gcggttcaac ttttcggtcc tgtcgtgcat
    33661 aaccataata agggataact gcagtaatac gctgagcaga agcacgcatt gcggcatcta
    33721 ccatcaacag caattccatt atgttatcat tggcaggatt gctggtagat tgtattaaaa
    33781 aaacatcaac gctgcgaaca ttctccagaa ttttaacaga tatttcacca tctttaaatt
    33841 tttttatttc aatttcagaa agctcaatac ctaaataatg agctatatcc tgagctaatg
    33901 cataatttga tgttcccgag aataatttaa ttggataaat catatacacc ctatttttta
    33961 ataaacgttt ttgtaattat gcttccaagt tttataagtt cttctttaga atttacacca
    34021 gttccttcaa tggggtcatc aagtttaatg attttcacat catatccaga ctttctgata
    34081 aatgttaaaa catcaggtag ataatattcc ctttgagcat tgtcggtggt aatagttttt
    34141 aacccctgga aaagaatagg acttttaaaa gcataggtac cggtattcac ttcttttata
    34201 ttcctctgaa taggatctgc atctttttct tctacgatcc taatcaaatt atcatattca
    34261 cctcggataa tacgaccgta tccgcaggga ttttcaagtt ccattgtcag aactgcagca
    34321 cctgtactgt ggtcgttaac agcattatac agcttgataa atgtttcagg ctttatgagt
    34381 ggcacatcac cgcatgcaac aagtaaacaa ccgttatagg tgcccaattg aggttcagtt
    34441 tgcattacag catgacctgt tcctaattgt tctatctgcc agacaaagga gatattgttg
    34501 tcaattgctg aaattacatc atcaccttta tagcccacga caactataat atcattataa
    34561 cttatacctg cactgatgag attatctata acatgctgta caagcggttt cccggctaac
    34621 ctgtgcagta ccttgggcag gctggattgc atgcgcacgc ctttacctgc agctaatatg
    34681 acagctttac agttattcat aactatcact cataataaag tatgctgagg tgccaggatt
    34741 cgaacctggg aatgccggga ccaaaacccg gtgccgtacc acttggctac acctcaaaat
    34801 aatattataa ttatagtaat aaaatgcctt atgtcataat aaatttgata caaatacttg
    34861 tgaatagtga ggtttaagcg tcaggaaaac ctcattggct tttccaatat cgtgaaaaag
    34921 tgcacataat gccgaaccgc tacctgtcat atggataaaa tccggggcaa aatcctgcaa
    34981 ggtgcggtaa atttccctaa gctgtggatt gttatcaaaa acaatatcct ggaaatcatt
    35041 tttaaaaatg tttaataaag cacctacatc acttttatcc cataaagaca aaactgcttc
    35101 tttttttgac aaaacaggcg caaaacttct catcaatgcg gtgtatgctt ctttggtact
    35161 gctgtgaacg tcattaaaaa caataacaac tgcatctggc agaggtattt ttttcaatgg
    35221 agttactatt tcacctttac cttcgcaaag agcaatattt ccatttaaac aaaaaggaac
    35281 atcagctcca atctcagcag ctaacgccat aagttcattg tgactgaatt tattgaatac
    35341 gttattacag agcatcaagg ctgctgcagc atctgcactt ccgccaccca atcctgcacc
    35401 tgatggaatt tctttttgta tggcgatagt aacccttgag ccaatatgtg cagttgaaca
    35461 atataaacgt attgcttttg tgatcaggtt ttgctctatg gggagttctt ttactatagc
    35521 ggcattttgc ccgcctatgc atcgaatagt tatatcgata gtattaggat tgcatagttc
    35581 acatccctca agttgtaaaa gatcaaagat attaatcttc gccatgatac tacagatatt
    35641 atggtagcta tctggccttt tgttcaaaac ttctagatga agatttattt ttgcgtaggc
    35701 ttttatactt tcaatcatca ataatggtat tgtgtaatag attggcaaat ctatatttta
    35761 gctgtttttc tgtcaagcaa ttaatgttgt attaacaata tagttggtgc aggttcaagt
    35821 ttagcaggaa agatgtatga ttgtagatca agaataatta attatatata aaattacaac
    35881 ttacaacaaa acacatttga agcctaatgt taatcatttc catgttataa ttatgacatt
    35941 ttgtaataat aattgacaag aataccgcta tcttgtattt tattttaaca tagtaacctt
    36001 taataattag ttttgaacat tcccttccgg ggataagtat tggaatactg tgtatcgtat
    36061 tggtggtaat gaaaagctgg attcccagag gttcccataa gcaaatagat atgagttaaa
    36121 taaaacaatg aagaatagta tcataactac ccatagttta aatggcagta ttttttcttg
    36181 tattaaaaat attcatggac taagtgcaca aggatgaagc tacttttttc ataacgtagt
    36241 acgctattat gaatatcaaa atttatgtta gcgcgttggc tatgaaatta tacctcattc
    36301 cactattagt agcctgttta ttctttgcag gttgctctcc gacagaggta tttttttcaa
    36361 gttattctgc acaatgtgga gtatttgatt gttctacaat tgattttact aaagagaagc
    36421 ctgttgagct taagggtgag tggaagtata gaatgctgga agatagtccg gcttttgctc
    36481 gaccagatta cgatgattct gaatgggatt atatcataat acccaattac tggaattctg
    36541 aaaaatttaa aggcgatggc tatggctggt atcgcttaaa ggtaaaagta aataaaagtt
    36601 tttgggaaac ttcaccagcc atctatgttc cagg
//
LOCUS       DAOSOX010000005        35611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1231_length_35611_cov_8.430926, whole genome shotgun sequence.
ACCESSION   DAOSOX010000005 DAOSOX010000000
VERSION     DAOSOX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 35611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_35611_cov_8.430926"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1115
                     /locus_tag="PLX22_00800"
     CDS             <1..>1115
                     /locus_tag="PLX22_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HOT18473.1"
                     /translation="FKKNIKPITYIQVPKAWNKQEDVSYSFPAFGYATCRLMILHNVK
                     FAGQIKTIKMPFLHTAYSLWANGKLISENGKVGTSKESMIPFQLPTITQFAIDSSTTE
                     LVLQISNFQQRNGGILRHIYYGNFNPINKHHEIDLFITLFVVAALFIVLIYHLGLFVI
                     NRTYHPNLYFALFCAIIAIRVLLTDEKLLVKLIPFLPWEIYLRLEYLTIMFGMVTFAH
                     FVNELFQNKLNTIILKSIDIYFGFFAIFVLVVPVMYVSYSLIYINIALVLSGLYMLIV
                     MIRETNYNSPNSLLYIFGVTFLFFTLIHDILFAQQLINSVYLLEIGLLIFVFTQSLVA
                     LEESIKIQNRLVTINSELEIARKIQKTILPEVIPSTQ"
     assembly_gap    1116..1215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1216..1428
                     /locus_tag="PLX22_00805"
     CDS             <1216..1428
                     /locus_tag="PLX22_00805"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18474.1"
                     /translation="RIILYTDGIVEIRNMKNEIFGLDRFISYIEKFYYLSPDEFIDII
                     IDALIQWAGNKNRITDDITLVIVDIK"
     gene            complement(1536..2708)
                     /locus_tag="PLX22_00810"
     CDS             complement(1536..2708)
                     /locus_tag="PLX22_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT18475.1"
                     /translation="MLVKRIKIDLDYLKPNSSFIYPLISEEGDIVLPARVTLTSDKIN
                     IIKARYGNILYYNDHGHMAVIPTFRMQIARNKSKEIMNEIANSHKINKIAFHEAEMVV
                     TDIVNDLTSTDIVALNLLKDLKTHEEYIYNHSVNVGILSAVLARMIGIFNKDEIKNIA
                     VGAYLHDIGQMQLDKNLLEKEGKYNITDIQKMKRHPQLGYETLKKIKDVHPIVLQSIL
                     FHHERYNNHGYYALPYENLPVYPKIVGICDIYDALTSKRPFRNAIEPSNALKAILNAI
                     NIHFDYQLVNIFINKLGPVLNNTQSFYTHNDICELNTQELAIIKDFGIDDMLKPKVII
                     FCKFHRTGEQIMARFYDNPLEIDLSHDPDNRYMIKIINNYKQLKSIQKKLEEKNLI"
     gene            complement(2888..3433)
                     /locus_tag="PLX22_00815"
     CDS             complement(2888..3433)
                     /locus_tag="PLX22_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18476.1"
                     /translation="MAKGTTLFKAYMNNSIPSDGGFIISAFFNSDSIYSIYEVTAYKN
                     VKDIYETAEGLTFKTDGSRTHLLTEPPTYARRYEEPVHRETGKSIPYRFDDLTILTGQ
                     KQEKIMIPKEPLMLYTSFTILKAQGDNFSFIFHPTEDVYTAIRKFIADSLYNDCGLTK
                     EDSLNAAQILLQTIKSFKVFS"
     gene            complement(3532..4155)
                     /locus_tag="PLX22_00820"
     CDS             complement(3532..4155)
                     /locus_tag="PLX22_00820"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT18477.1"
                     /translation="MASLTQLLFDFAPYVHFVAFFLLILAGFNLPISEDIVFIISASI
                     AATIVPENTFKIAAACVLGAYSSDIIAYCLGRYAMKFILQHKKLARFYPMEKINKIES
                     YYTSHGAKTLFFGRFIPFGVRNVIFLTAGLSAMKFFKFCIVDFMALSITSTILFTSGY
                     LLGANYEIIFPFLNKFKIGIFIIFVAFLSVLFIIKNKKIFYLKNFKI"
     gene            complement(4179..4505)
                     /locus_tag="PLX22_00825"
     CDS             complement(4179..4505)
                     /locus_tag="PLX22_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18478.1"
                     /translation="MEEQKKLLELLQKSQTTIIDKDRKINFFEMHVAFIGTPKKHQYD
                     KSKIILLSDPFSEKKFFYEFPVEAIGLVEELGTISSDDGKNALQIRLWIKKGTIYFKY
                     EPFIVD"
     gene            4887..7028
                     /locus_tag="PLX22_00830"
     CDS             4887..7028
                     /locus_tag="PLX22_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOT18479.1"
                     /translation="MHSIIQNIFEKNFNKAIQFDDITVTLGGNLKLKNIKIAPTTDFN
                     DNYNLISSKKTIIDLNYFKAITGRIHINGIKFYDSSITIVKNYGKSYKEIFDHISTNV
                     ATNAKYISYEDFYIKAMGNCIYNENFSNDKLKINIGDVCISISMDDTTFRYHIKGKVK
                     PLDDRLDEGKIKFSGYVYFSDKMKYQSSSHEIYIKKLDMSIANYFIKDYSDLSLSIRG
                     YFYTNAMIKHDNTYSIKGSVDFDNITIENTGVLPHYMIVSNDNVSIKSSMKINNDLSH
                     VIIDYVSFNDNEIDINASLQFLKDKYFNIAVTTNTIDLDDIDYLQIIPDVYGDGTFAL
                     DIQCDYDLSHNEMRSFKIYCDAEDIAIFSKTKNNAMTYIKDSWLKINGDIKKININAC
                     AKHQKSDVMLRSTINIEKWVPFTSTDEIMVQSKRLSAELLSKLLLKGIKELYSKAAED
                     LSIGYNEIYFRQKPLGKYLSNNIINTKINVDTLTFEKNAHLKNINIELLSNKGLVTTQ
                     SFNCEGYSANYNFSVNAFCNSDYPTMTISCAVNNFDYGTFLRDTGLKDATGLMNMNLN
                     YGVSGYRLSHLLQNGNGNFTLNIANSVLNSSMIQRKIIELAKSCNIVVEGGGWHCNRI
                     DVAINHTADRLILQSLYIDTDNASVSGYGNYSIENGLSLPCNATFYKRDGRTITTTHR
                     ASFSIKGELLRPFIVSSFPCGKEQILLFDVN"
     gene            complement(7008..8996)
                     /locus_tag="PLX22_00835"
     CDS             complement(7008..8996)
                     /locus_tag="PLX22_00835"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT18480.1"
                     /translation="MQINKSYLLGLGLSIAVFLIISFFYAQTKLLDGLEYGSINFRFF
                     LRDPSEKAIKLQEGVKMTRKNPRSRDDIIILGIDENTIREFDNEGIKWPFPWLYHAKF
                     TQYVASGNPVAIFFDIMFLDHKPYEDEFAKAIKQANCVFLDYPFETEEVDVKYSDIEE
                     RMNLLNIYAMTIDPNDNSMPWVEEAVPPTPLLSQAAIGLGFANIKPDSDNINRKVPLV
                     VKYKGLYYPSIDLLLVMHYYGISKKDVEIKVGQYIKLSNIPKEKMVKTTHDNSIKIPI
                     DAEGFMDINFIGGPGSFTHYPYYYFCRDGIITNTSLQDKIILVAAYSSTGISTDIHKS
                     PYGDLFGIEHHANALNTILNQDFIVKLTPFQNILILFAIAILLGFILTRVSIIISVLF
                     TVGLSLLYIIGSYLLFDAFNIITIFASPIIQVGTTFSIIIAYRVLTEQQEKKYIRQTF
                     SKFVSKSVVDELLKNPENLKLGGEKKFLTVLFSDIRSFTTISEKMTPEELVEHLNEYL
                     QAMTELVFKYDGTLDKYVGDEIMAFWGAPIPQDNHALMACKCAVEMIQVLEQLNKRWI
                     QMNKPALHIGIGINTGEMVVGNMGSASRMDYTLMGDNVNLGARLEGTNKQYQTNIIIS
                     EFTYEQVKDYIIARELDLVRVKGKQLPVKIYELIDIKQ"
     gene            9219..10886
                     /gene="argS"
                     /locus_tag="PLX22_00840"
     CDS             9219..10886
                     /gene="argS"
                     /locus_tag="PLX22_00840"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT18481.1"
                     /translation="MIIKEYIAQIVDKALQKVLQAHDILQQPSLSIKIEYPKEEKFGD
                     YATPIALECARILKTSPLQIGEQIKGFIDEQHIFERIDVVKPGFLNMFLSLPFLVQRL
                     QAIVTANDDYGRNKKEYPRKVNIEFVSANPTGPLNVVSARAAAVGDTLANLLAASGDT
                     VNREFYVNDYGNQVWWLGKSVWVRYQQMLGKDVIFPDEGYHGEYIKDIAKYIHENFSH
                     ELSNINDEEKAVQFCARKAVDYNIAWQKQDLERFNVHFDTWFYESTLHESGKVLDVFN
                     YLESLGVITDDNGKKVFVSTKFGDDKDRVVIRDDGRPTYLMADIAYHRTKIERGFDLI
                     IDIWGPDHHGYIARLVGAMKAMGYDENQFRILISQQVNLLTNGEAVKMSKRLGTFSTM
                     HDLIDDIGTDVARYFFVMRSMDSHLDFDIDLAKRQSSENPVFYLQYAHARICSIFREA
                     QNRNINYDKIIVDTAHYSNPETITLLKLMARFPEEIYDAANSYEPHRITVYCMRLAQA
                     YHKFYTEHRVLSDDHDRTQSYLILCDGVRIILKNALSLLGVCAPERM"
     gene            10970..12190
                     /locus_tag="PLX22_00845"
     CDS             10970..12190
                     /locus_tag="PLX22_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOT18482.1"
                     /translation="MTKVALIATGSELVNGRIHESNSYYISRQLFSIGLPVVAHYVVG
                     DDSASLRSIIEHAMYSADIVFITGGLGPTVDDITCETLQSMFHLNLTVHKPSKARMDY
                     FFKATGKSALDTDVKMVTVPENAYVFPNENGLAPGFAIGYDNKDIIVLPGVPHELQSM
                     MMSVIEYCKKKYQLQKKEELTFKVIMMREAEVDSAVKEIVNKQENIDIAITYESGTTT
                     VILSPKSGNVLPAVDIVDEIKKRFGSKLIFGYNSLEEKVVHLLAKNKLRVAVAESCTG
                     GLISKRITDIPGASSIFAGGVIAYSNEAKKTLLGVKVDTLNRFGAVSRETALEMAWGV
                     KQRFASDFGIATTGIAGPDGGTKEKPVGLVCFGIAGSDTFSTQIQFSGNREHVRTLAA
                     NYILNKLLTFAGIE"
     gene            12193..15015
                     /locus_tag="PLX22_00850"
     CDS             12193..15015
                     /locus_tag="PLX22_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18483.1"
                     /translation="MKRRVIVLVILLHSIVCSLAFALESSQLFEQGMEAFRMGNYGSS
                     ELIFRKIIDANDSYKDRAWYYLALSIFYQKQYRSAIFELNRFLLLCTTPDLCNEARYW
                     IAESYYYLNDYIKAIEEYNRFITQNKNKQLIINSYDRIGEIYFNQKRYDEAIIEWRKA
                     LTLTSKKFEDSNRVIKIGEALFLSERYSEALDILEPLIHAVNDATISSKAQLIVGRIY
                     QIQGKHWQAIKYLNEIPESMIKIPAFLSVQYFKALSYIALNDIYNARLNLKSYILISN
                     EDSPYYYNARYELGKILLNTKDLDEGISLLADVQVNSNDIDLRSRASFELGQLYLKNN
                     TIEKAIPYLENAISINDQESQKYVMMTLSELYIATDRYDDAKRLLDFMLEKYPYEENR
                     DEMLYLEAKIYILQGDVEKSLEYFELIKEVNPFSKYLVELDYAQAVDFFKRGDYTSAI
                     ASCNRYLNNYKVDNRYEVLIILSRSYIKINNFKKAVETINQLIAKFPKKNGLDEILYE
                     LSLSLNEKGVNVEKYWNFIIKNYYSSITAGKIYIEKGDEAYKKKNYQSASYFYSQYLS
                     IKGRPRERSVLLFYIISLYELKQYNTIIKTLKDSEYIAVDDYTTKLLIKWLAKSYYML
                     KDYTIALNTFMKLPLMDYSMDDLVLISDTALKNNQPDIAIETLQYMEQDNRYNEVAYN
                     IALYYKNNGNIDLATEYFKKVLIKKHHGMVLSNAQIQLAQIYLEQEQFDNALELVKNI
                     QDKENIIMRDSIAFIAHYKLGNINEALSIINNKLNDINMTAFADEIFTLLLQHYYKQN
                     DIKNLKIIYSYSSKRKKLISVSNYYYGLIHYINNNYNQAINYFTIASRFQDEYGISSR
                     YYLGIIYFAIQKNFVTAKRNFDRYFEKAEQDDINYYNAKIYVSMLLFETGKKDESKTI
                     LYEIVNNEGNILAKIKATHLIKHYGF"
     gene            15078..15527
                     /gene="aroQ"
                     /locus_tag="PLX22_00855"
     CDS             15078..15527
                     /gene="aroQ"
                     /locus_tag="PLX22_00855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT18484.1"
                     /translation="MKQYTFHVVHGPNLNMLGTREPGIYGVFTLDEINNKIIEYAKSH
                     NITIHCFQTNSEGEIIDYIQRNKNCDGIMINPGGYTHTSVAIRDCIAGCNTPVIEVHL
                     SNIHAREHFRKHSYIAPVCIGQICGFGYYSYILGIEALVYYLNNIQK"
     gene            15538..16614
                     /locus_tag="PLX22_00860"
     CDS             15538..16614
                     /locus_tag="PLX22_00860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOT18485.1"
                     /translation="MNYLSRIKKIRNKLKNNDCFPYLVSSLINIKYCTGFDGSYGYLI
                     FFPDETVFISDSRYEEYAISILPQNVRFVLQEKDINQTLSALLKKKSVKKLFLEEHAI
                     SLSQYKAMKEHCRGIALEYGGDSINELRVIKEEEEIEIIKQAAKLTDLCFNHILNILK
                     PGITEWDVATEIEYFYKKNGCLGTSFATIVASGNGSSMPHYKTDVKIIKEGDPVLIDM
                     GCIYNGYCSDITRTVFVHSASEDIKNIYVIVKKAQENAIAAVKPGMTTNKLDSIARDF
                     IAAHGYADKFGHSLGHGVGMDVHEIPAIKQNGMIKLKKGMVITIEPGIYLPKRGGVRI
                     EDMVLITNNGYEVLTKSPKDIIVL"
     gene            16845..17408
                     /gene="efp"
                     /locus_tag="PLX22_00865"
     CDS             16845..17408
                     /gene="efp"
                     /locus_tag="PLX22_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT18486.1"
                     /translation="MITSNDLRNGTVIKVDGDLYSVVDYQHVKLGRGGAFVQTKLKSI
                     TKGTIVDKKFKGGEKVEDVRIEKKTLQFLYDEGDALIFMDTETYDQLSIDKKKAGNLL
                     NYIKEGDNVEATMHNDAILSIEPPIFVVLKVTYAEPGVKGDTATNVLKPVTVETGATI
                     QVPIFVNEGDTIKVDTRKGEYVERVKR"
     gene            17464..18615
                     /locus_tag="PLX22_00870"
     CDS             17464..18615
                     /locus_tag="PLX22_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18487.1"
                     /translation="MKSNIITKEAYKAFKKGETSVVIQLLEKNIAISNDPYDFFLLVI
                     AYLLTDKLVQAEATLRKALRQHQNYIPLLEIKAFLILKAAKTKDDACSGYVDIIKLSP
                     NNKKITKIIKTVHETDNFEKFQKQARIKDFITIQPPKKHAMHKHQHSGRARFPVMKCV
                     LLFSIIAIIIAGIIFFSSPLAKLITQKISLLFTETRSDIHELSNITLDGMHYDVIDTI
                     QKFKTPEFYTSGEECIRDFNHAKWLIKEGKENDAMIILNKIDASNVQMKVKQRVAFLK
                     DFIIRNRERTVSGYLYNDVIKKPYLYKGINVKWRGKVANYKKQGRNHTFQLLINYEND
                     RFDGVVEVFSSTADNSITNGTIANVEGIITNIVAQKIIVLHALNIEIVR"
     gene            18654..19799
                     /locus_tag="PLX22_00875"
     CDS             18654..19799
                     /locus_tag="PLX22_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT18488.1"
                     /translation="MEVILKECKTYNDDIVTKQITSALNALHFDFSRFYNAKVAIKPN
                     LLTSTAPEAAVVTHPLFFRAIVHVIKQHGGIPILVESPAVHSLQRVMKKAGYDAIVAD
                     EEIQVADTSDYIIIHNENARHFKYFEVPKIFTECDIIINLPKFKTHALTYITCAVKNL
                     FGTIHGMKKSQWHIKAKTKSEFSEMLLDLYEAYITDSSLPKTIIHIVDAITILEGDGP
                     GLKGNPKFMGIIGASYNAIAVDYALSKIAGFTIDEIPTITMGMKRNICAVSDAITIVK
                     DAAIENNYNCIPPKESGSQSILSISIVNRLLKNLIIAKPLPDDNKCTLCYQCKQICPA
                     KAISIAKDGETIPNYNYSKCIRCYCCMEICPESAISLSQPWLQKLMR"
     gene            19809..20195
                     /locus_tag="PLX22_00880"
     CDS             19809..20195
                     /locus_tag="PLX22_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18489.1"
                     /translation="MKNKYTLKKIVIIEFIVFLLLVMFFWFEEILDLPYIIFNEEPTP
                     INFVECLMETVVITILFGILLYETIKINLSMGKLESYIYVCARCHKIFINGEWISLDK
                     YFNEYANKKTSHGLCDVCKRNYTKED"
     gene            complement(20193..20266)
                     /locus_tag="PLX22_00885"
     tRNA            complement(20193..20266)
                     /locus_tag="PLX22_00885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20230..20232),aa:Met,seq:cat)
     gene            complement(20271..20343)
                     /locus_tag="PLX22_00890"
     tRNA            complement(20271..20343)
                     /locus_tag="PLX22_00890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20308..20310),aa:Met,seq:cat)
     gene            20707..21114
                     /gene="speD"
                     /locus_tag="PLX22_00895"
     CDS             20707..21114
                     /gene="speD"
                     /locus_tag="PLX22_00895"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOT18490.1"
                     /translation="MEGLGTHIIAELFNCNAFHINNLKKVEEVLTAAAELSKATIIQP
                     FFHKFSPYGISGVIVIAESHITIHTWPEYGYAAVDVFTCGDHDYKNAIDYITEKLEAD
                     RCVLYEMRRGVMNDSKVKCIPVIREVENVNYIE"
     gene            21161..21511
                     /locus_tag="PLX22_00900"
                     /pseudo
     CDS             21161..21511
                     /locus_tag="PLX22_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     assembly_gap    21549..21648
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21702..22508
                     /gene="speE"
                     /locus_tag="PLX22_00905"
     CDS             21702..22508
                     /gene="speE"
                     /locus_tag="PLX22_00905"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002182584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOT18491.1"
                     /translation="MKLKVIDKLVTYDSKFQKIEIYETKAFGRLLVLDGVFMTTELDE
                     MGYHEMIAHVPMCVHPAPKRVLVIGGGDGGTLREVLKHKEVEKADICEIDGDVIRLCK
                     EYMPALSCSFDDPRVTVYTEDGAKFIAERKNAYDIILVDSSDPIGPAAVLFSEQFYKN
                     VSQCLDSDGIASTQSESIFYHSNIIVTLANYNKKYFKVPSYYYTMVPTYPSGMIGFSF
                     CSKKYHPLNDFIQERAQVLLPQLQYYNPDIHRGAFALPQFIKSKLTGIIV"
     gene            complement(22588..23301)
                     /locus_tag="PLX22_00910"
     CDS             complement(22588..23301)
                     /locus_tag="PLX22_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT18492.1"
                     /translation="MDFTIVLYQYPLGSFITHHTFQNLQRIKPHFICFPEYFFVNKKL
                     GNHAQTYHNQQLQLKRIQLLSRELDTVVIGGTMPELNGNKLHNTTFVFHNGTILGFYR
                     KQNLFFAEEGKITPGNGHVIFNAYGIRFSVLICADVFKDENFIALKEQGVQIIFIPTF
                     SLFRNETIDEKYKRDNDIFIRGAKLADAIIVKVCGVKSDYKNFLQARSLIASPEGILY
                     RVEPEQEDKSMLIVNTITI"
     gene            23525..26077
                     /gene="glgP"
                     /locus_tag="PLX22_00915"
     CDS             23525..26077
                     /gene="glgP"
                     /locus_tag="PLX22_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOT18493.1"
                     /translation="MGTIQTFRVYPYLPDNLKPLKTIAYNLWWVWNFEAIELFRRLNV
                     ELWRDLDHNPVALLGMLSQSELDEAADSESFMAHLKRVSEELEWHLTKKSWVEDAYPT
                     YKNMQIAYFSAEFGIHECLPIYSGGLGVLAGDHIKSASELGLPFYAVGLLYRLGYFHQ
                     YLNIDGWQQERYPETDFHNLPLQLILKDDKPLVVEVEYPGRIVFAQIWECNIGRVKLY
                     LLDTNITQNNLEDRTITDQLYGGDVEMRIKQEMLLGIGGYRLLKALGKNISVYHMNEG
                     HSAFLSIERIHDTMHTHKLNFYEAIEYVNASTVFTTHTPVPAGNDRFVPELIDKYLSH
                     YFQKLSISRDEFLALGRENPHDKIETFCMTVLAIKTAAHCNGVSKLHGSVSRRMWKNI
                     WPGVPVNEVPIYHVTNGVQILSWTSDEMMRLLNRYLGPRWIDNPVDKTIWKGIDTIPD
                     SELWRCRERLRERVVAFARKKLREQLIARGAPRAEIEKADSVLDSEALTIGFARRFAT
                     YKRATLILKDMQRLERILLNKEKPVQIIFSGKAHPRDNMGKELIKRIIHLGRDDRFRS
                     KIVFLEDYDINIARYIVQGVDVWLNTPIRPQEASGTSGMKVVPNGALNISVLDGWWPE
                     AFNGNNGWAIGKGEEYHDPDYQDEVESLSLYNILEQEVIPLFYNRGADGIPREWANRI
                     KESMKTILPQFNTNRMVKEYTEMYYLTAHDNFVKFSKDDFKASRSLAKWKKDIMQKWH
                     QVTLESITYESKKEIIVGSQLKAKAVVNLGDLKPTDVQVELYFGNLNQKGEIEDGAAL
                     PMHMVEESGGGKYIYEGRMLCLKSGQFGFTVRVIPLHSDMPRKYESRLITWA"
     gene            complement(26095..27891)
                     /gene="lepA"
                     /locus_tag="PLX22_00920"
     CDS             complement(26095..27891)
                     /gene="lepA"
                     /locus_tag="PLX22_00920"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT18494.1"
                     /translation="MEQSLIRNFSIIAHIDHGKSTLADRIIERCKAIDPRNHVDQLLD
                     DMDIERERGITIKANVITLNYQSKDGKIYTFNLIDTPGHVDFAYEVSRALAACEGVLL
                     LVDATQGVEAQTIANMYLALENDLTILPVINKIDMPNADIQRTKESIKKSLGLDPDTV
                     LCVSAREGIGIDELLEKIIEIIPAPKGNPSAPLKALIFDSYFDQYVGVVTKVRIFDGQ
                     VQEDDEIMLFSTQKQYEVTEVGHFRLKREKRVQLTAGEVGYITAGIKQLTDTRTGDTI
                     TSAENPVSHPIIGFKEAKPMVFSGLYPSISDDYDDLKDALYKLKLNDAALHFEPDNSY
                     ALGFGFRCGYLGLLHMEIVQERLEREFNLALITTAPTVEYRVIKTTGEVLLIDNPVKM
                     PDPSQIERIEEPFVQATIITPSEFIGNIMALVNECRGIHKKMEYIDPTRVELFYEMPL
                     AEIMFNFYDKLKSCTRGYASFDYELIDYRASDIVKVDILVNEESVDALSFMVHGDKAY
                     KRGREIIERLKEVIPRQQFKIPLQAAIGSRIIARENISSLRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRLKQIGSVEIPQEAFLTILKIE"
     gene            28044..28499
                     /locus_tag="PLX22_00925"
     CDS             28044..28499
                     /locus_tag="PLX22_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOT18495.1"
                     /translation="MKVLVISDTHGNIDAIKTIIEAERKFDYLLHCGDGINDLMYVSV
                     PEGVFVITVCGNIDRARGINEKSTIVETIGNFKFFITHGDVYGAHHDITGVWIEAKRS
                     GCDIACFGHTHKPCNTNGSPMMFNPGAAQSGFYGIIESGDSLKAVLKRI"
     gene            complement(28533..31592)
                     /locus_tag="PLX22_00930"
     CDS             complement(28533..31592)
                     /locus_tag="PLX22_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT18496.1"
                     /translation="MNSDINFTENADHVKYLLQIANLDTTSSLSEYVHNLKTTIAQLK
                     IPVFNISCLSNIEGTDHLLIFPDWDSLKQWVPELSISNNIFEYATINIKKLIANRAIF
                     KKSILHSLLRGYLTLTDTISIPEVIQYHKAIVSCEQMISDFSQASKELFQILNPDVQN
                     RILESPLFGSAISFPLFSDRDVIGSFDVVYPKNYPEINDNEFATLELIAKLIASGLRQ
                     YRYQNKIIISETKYSSLVNAIPMALFVINKEYSIIETNDTFKKWFNIERPLNKKCYEI
                     VGCSNKTCNGCPASKAMDKAAPILQEKKVPYFKDTRFFNYITSPIIAPSGSISSVVVI
                     MEDITTRINAEKKIEKFSLRLAEEVDNQTKQLKEKQQKLQLTTNTLYALKQANTIKES
                     IHCIIESLHTLGCKYCIVTLLSSDVNTLQLVEVYPASFLQLLQAVTNQNITTIIFDFD
                     DSANIPFFSVIKSGKEIMIEHFSELISFLESTFPQIESDKIKQISSLLKDEVIGIYPL
                     GSKHQIEGSIAIALDKQSIEENKDYIEILLNAAAVEINRKRNQEELVKSELKYRNLVE
                     NTQDIIMLCTAEGEIIYGNSAFYKLLAYPETRKPSFFNFIDDNEKYLIQSIMQHLLNY
                     GINPDPFEIQMYTCDEKFLWFEIAMSMIPLDHTVGIQIVARDIERKKRMESHIQNLTE
                     FQEKILQNEMIGIITTDLSGIITSWNKAATSILQYQPFETLNTPLQNLILNYYEDSVL
                     SKSNTFASIINQPYTEIKMLKKGGTIITTLCMSSLLHDDHDMPFARLHFFIDISEKKQ
                     LEAESLELNKKLQHAQLVTIVSLAKLAEYRNKETGMHLERIMRYVEILARELSQHPDY
                     RDYITEEYIQDLVNSCLLHDIGKVGVPDHILLKPGKLTDYEYEIMKTHTIIGAETIAE
                     AEKKVEGRSYLNLGKEIAYYHHERWDGSGYPKGLKGDAIPFSARIVAIADVYDALVSE
                     RPYKRAFPHEKAVEIISKKAGTYFDPVIVEAFIKHHNRFLQIKETLL"
     gene            31888..32334
                     /locus_tag="PLX22_00935"
     CDS             31888..32334
                     /locus_tag="PLX22_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18497.1"
                     /translation="MEIFKTFNNNGIHYIIVGGIAVNLYGIPRMTYDIDILLYLEETN
                     TKRFVEQMIQWDFKPKIPVKPMDIVDVNKRNDRIKNKSMKAFCFANPDWAISKIDIII
                     DSPINYEEAITRAKFVTINGIKLPIISIDDLITMKKVSMRDKDIVI"
     gene            32356..32547
                     /locus_tag="PLX22_00940"
     CDS             32356..32547
                     /locus_tag="PLX22_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18498.1"
                     /translation="MNDNTNKGFRYYIDDATLNEYQQKHYHLRLQWLYMGNKLRKGYS
                     STIKAIQDSFRRGEGSFKQ"
     gene            complement(32561..34738)
                     /gene="feoB"
                     /locus_tag="PLX22_00945"
     CDS             complement(32561..34738)
                     /gene="feoB"
                     /locus_tag="PLX22_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT18499.1"
                     /translation="MTKKTITVGLIGNPNCGKTTIFNALTGARQKVANWAGVTVERRE
                     GFFNHKGYTIKVVDLPGAYSLTSFSMEELITRNFIQNDKPDFIINVIDAGNVERNLYL
                     TTQLIDIEAPVIIVLNMIDEAFDKGLIINIQKLSQLFNMPVIPTVGTKGTGIPQLKDS
                     IVENFEKQTKNHTITIPYGNDIEQAIQQLQQLIDKNRLEYPSRWLAINLIEGDIDAEK
                     RVQLLPDGNEIIRKAYTIRNNLSKAFHDDIQTVFADMRYGFITGALKEAVQKKPIKRH
                     DLTKKIDSVVLNRFLGFPLLFVILFILFNLTFILGEYPMKLIEVGVEWLSHYLMEIIP
                     PGELQNCIVQGIVGGVGSVIVFLPNILILFLGISFMEDTGYMARSAFLMDKIMHSMGL
                     HGKSFIPLVMGFGCNVPAIMATRTLENKRDRILTVLINPFMSCSARLPVYILFAGTFF
                     PNHAGFIILFIYALGIILAFTSAKLFNNTILKGKSTPFVMELPPYRMPTTKTIVIHMW
                     DRASSYLRKMGGIILAFSIIIWVLSEYPKPYHIQKQFDTMIQQKIIELNQTLSTYQKQ
                     NADPNTIQTIHKQYNTIIEDLEIQKRQEMVQYTFIGRIGSFIYPLLQPLGFNWQMGIS
                     LTTGFVAKEIVVSTMGVLYHAANDENTVSLSQKLGNPRYGITPHAALAFMIFVMIYIP
                     CLATVIAIAREIGTRWAFFSVAYQITVAWIVSFIVYQIARFIL"
     gene            complement(34745..35458)
                     /locus_tag="PLX22_00950"
     CDS             complement(34745..35458)
                     /locus_tag="PLX22_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOT18500.1"
                     /translation="MRKRKSSPWVNNPTNELNDTGLTANMEDYMENIALLLNENDVVR
                     VKDIAQKLNITMPSVTAALQKLKEKGLVDYEKYGHVQLTPRGKEIADSVYHKHSFLSE
                     FFQSILNMDKKSAEEQACKLEHHLSNDASTRMQQFVEFYKHERQANEQWIKRLDITLE
                     ERRLSQLKEGQKAEIVAILGSGPLKKRLLEMGFRKGEIIHIIKYAPLHDPIEIEIKDF
                     LISIRVEEANYIIVKPIDE"
BASE COUNT        11937 a         5287 c         6395 g        11792 t
ORIGIN      
        1 attttaaaaa aaacatcaaa ccaattactt atattcaagt accaaaagca tggaataaac
       61 aagaagatgt ttcgtattca tttcctgctt ttggatatgc tacttgtcgt ttaatgatac
      121 ttcataatgt caaatttgct ggacagatta aaacaataaa aatgcccttt ctacatacag
      181 cttatagttt atgggcaaat ggaaaactaa tcagtgaaaa tggtaaagtt ggtacttcaa
      241 aagaatcaat gatcccattt caactaccaa ctataacaca atttgctatt gattcttcta
      301 caactgaact tgttttacaa atatcaaatt ttcagcaacg caatggtgga atattacggc
      361 atatttacta tggtaacttt aatcctatta ataaacatca tgaaatagat ctttttataa
      421 ctttatttgt agtagcagct ttatttattg ttctgattta tcatctggga ctatttgtaa
      481 tcaacagaac atatcatcca aatctttatt ttgctttatt ttgtgcaatc attgctatac
      541 gagtattatt aacagatgaa aagttgttgg ttaaattaat acccttttta ccatgggaaa
      601 tatatttgcg attagaatat ttaacaataa tgtttggaat ggtaacgttt gcacattttg
      661 ttaatgagtt gtttcaaaat aaattaaata ctattatttt aaagtctatt gatatttatt
      721 tcggcttttt tgcaatattt gttctggtag tacctgtaat gtatgtttca tattcattga
      781 tttatattaa tatagccctg gtactttcag ggttgtacat gttaattgtc atgattagag
      841 aaacaaacta taattctcct aattcattat tgtatatatt tggtgttaca tttttatttt
      901 tcactttaat acatgatata ttatttgcac aacagttaat aaacagtgta tatctcctgg
      961 agataggatt acttatattt gtttttactc aatcacttgt tgcactggaa gagtcaataa
     1021 aaatccagaa cagactggta acaattaata gtgaattgga aattgcccgg aagattcaaa
     1081 agactatttt gcctgaagtt ataccatcca cacaannnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnaggat tattctttat actgatggaa ttgttgaaat aagaaatatg
     1261 aaaaatgaga tatttggatt agataggttt atttcatata ttgaaaaatt ttattattta
     1321 agtccagatg aattcattga tatcattatc gatgccttaa tacaatgggc agggaataaa
     1381 aataggataa ctgatgatat aacattagtt attgttgata tcaagtagtg tagcaatatg
     1441 cccatataca ttatgcatta taaataaata tgtatttaaa gtctttgaat acattactgt
     1501 aattaaaata ttatatatat attgattaag gttttttata ttaaattttt ttcttctaat
     1561 ttcttttgaa ttgatttaag ctgtttataa ttgttgatta tttttatcat gtatcggtta
     1621 tctggatcat gagatagatc tatctctaaa ggattatcgt aaaatcgtgc catgatctgt
     1681 tcacctgttc ggtgaaattt acaaaaaatg atcacttttg gttttaacat atcatcgata
     1741 ccaaaatctt taatgatagc taattcctgg gtatttaatt cacatatatc attatgagta
     1801 taaaaggatt gagtattgtt taatacagga cctaatttat taataaaaat attgaccagt
     1861 tgataatcaa aatgaatatt tattgcattc agaatagctt tcaatgcatt gctaggttcg
     1921 atagcatttc tgaaaggcct ttttgaagta agagcgtcat agatatcaca tatgccaact
     1981 atttttggat aaactggaag attttcataa ggtaatgcat agtatccatg attattgtac
     2041 cgttcatggt gaaatagtat agattgcaaa acaattggat gaacatcttt tatttttttt
     2101 aatgtttcat aacccaactg tggatgtcgt ttcatttttt gaatatccgt aatattatat
     2161 tttccttctt tttccagaag atttttatct agctgcatct gccctatatc atgaagataa
     2221 gcacctacag ctatattttt aatttcatct ttgttaaaaa taccaatcat tctagcaaga
     2281 acagcactta atatacccac atttactgaa tgattataga tatattcttc atgagttttt
     2341 aaatctttta gtaaattaag agctactatg tctgttgaag ttaaatcatt aacaatatca
     2401 gtcacaacca tttcggcttc atgaaatgca atcttattta ttttatgact attagcaatt
     2461 tcgttcatta tctcttttga tttatttcga gctatttgca tacgaaatgt aggaataact
     2521 gccatatgac catggtcatt gtaataaagt atattaccat aacgggcttt aattatatta
     2581 attttatcag atgttaatgt aacgcgggct ggcaatacta tatcgccctc ttctgatata
     2641 agaggataaa taaaactgct attaggtttt aagtaatcaa gatctatttt tattctttta
     2701 actaacacta gtacttattg cacacaaata tttttttcaa tataaaataa atatcaatat
     2761 taatcaatta ttatttttta ttatgtaatt aaaattttgc aaatttaatt gtgaatttta
     2821 tcgttaaaaa taaagtaaaa aactaaatta ttatagtata acttgtttta attattatta
     2881 tgcaaagtca tgagaatact ttaaagcttt taattgtttg gagcaatatt tgtgcagcat
     2941 ttaatgaatc ttcttttgtt aaaccacaat cattatatag agaatctgct ataaatttac
     3001 gtattgcggt atatacatct tctgtaggat gaaaaatgaa agaaaaatta tcaccttgag
     3061 cttttaaaat ggtaaatgat gtgtaaagca ttaatggttc ctttggtatc ataatctttt
     3121 cttgtttttg acctgtcagt atagtcaggt catcaaaacg atagggtatg gatttaccgg
     3181 tttccctatg aacaggttct tcatatcttc gggcatatgt tggtggctct gtcaataaat
     3241 gtgttctact accatcagtc ttaaaagtaa gtccttcagc agtttcatat atatctttaa
     3301 cattcttata tgcagtaacc tcatatattg agtatattga atctgaatta aaaaaagcac
     3361 tgataataaa acctccatct gaaggtattg agttattcat atacgcttta aaaagtgtag
     3421 taccctttgc catacatgcc ctcctgatta atttcacaaa gcataatatg agaaatgaat
     3481 atcatatgtt tatttataat atatgaagta aaagtaaaat gcaaacaaat tttatatttt
     3541 aaaatttttc agataaaaaa tttttttgtt cttgataata aataatactg acaaaaatgc
     3601 aacaaatata ataaatattc ctattttaaa tttattcaga aaaggaaata ttatttcata
     3661 attggctccc aatagataac ctgaagtaaa tagtattgta gaagttattg ataaagccat
     3721 aaaatcgaca atacaaaatt taaaaaattt cattgcagat agaccagcag ttaaaaatat
     3781 tacatttcgc acaccaaaag gaatgaaacg cccaaaaaaa agagttttag ctccatgaga
     3841 tgtgtaatat gattcaattt tattaatctt ttccattgga taaaatcttg caagtttttt
     3901 atgctgtaaa ataaatttca ttgcatacct acctaaacaa tatgcaataa tatcactgga
     3961 ataagctcct aaaacacaag ctgctgcaat tttaaaggta ttttcgggta caatagtagc
     4021 agcaatagat gcagatatga taaagacaat atcctctgat ataggtaaat taaaacctgc
     4081 taaaataaga agaaaaaatg caacaaagtg tacatatggt gcaaaatcaa aaagtagttg
     4141 ggttaaagaa gccattgttt attcttaaat tttggatgtt aatcaacaat aaatggttca
     4201 tatttgaagt aaatagttcc tttttttatc cataggcgta tctgcaatgc atttttacca
     4261 tcatctgatg aaatagtgcc taattcttca accaatccaa tagcttctac aggaaattcg
     4321 taaaaaaact ttttttcaga aaatggatca gataacaaaa taatttttga tttatcatat
     4381 tgatgttttt ttggtgttcc aataaatgct acatgcatct caaaaaaatt tatttttcga
     4441 tctttatcaa taatggttgt ttggcttttt tgtaataact caagtaattt tttttgttct
     4501 tccatgatat aaaaccgtac tttttagtaa ctcttttaat actatatata atcatatcaa
     4561 aagatttcta agtaactatc ccctataagt attattcttg tcaataatta tatcattgtt
     4621 atcatcattc tacaacaaat ctttgttaat ataataattg ttagtataaa tcatcaaaat
     4681 aaagtcttga caatttattt tactatgatg taatatttat aaatagctat ctcaagtaaa
     4741 ataaaaataa acaaaaaaaa atgaatttag tatataatca tattatctca aaacggtggt
     4801 caatattgta tattaccgtt ggagtttgtt ttgcattact gttaatccag aaaatgttat
     4861 ttaccctgtt attcaatgaa aaagaaatgc attcgataat acaaaatata tttgaaaaaa
     4921 attttaacaa ggcaattcaa tttgatgata ttactgtcac gttaggtggc aatcttaaat
     4981 taaaaaatat taaaatagcc cctaccacgg attttaatga taactataat ttaatctcaa
     5041 gtaaaaaaac aattattgac ttgaattatt ttaaagcaat tacaggcaga attcacataa
     5101 acggtataaa attttatgat tcatcgatca caatagttaa aaattatggt aaaagttata
     5161 aagaaatatt tgatcacatt tccacgaacg tagcaacaaa tgctaaatac ataagttatg
     5221 aagattttta tattaaagcc atgggaaatt gtatatataa tgaaaatttt agtaacgata
     5281 aattaaaaat aaatattggt gatgtatgta tatcaataag tatggatgat acaacattca
     5341 gatatcatat taaaggtaaa gtaaaaccac ttgatgatag acttgatgaa gggaaaatta
     5401 aattttcagg gtatgtatat ttttcagata aaatgaaata tcaatcttca tctcatgaaa
     5461 tatacattaa aaagttagat atgagtattg caaactattt tataaaagat tattctgatt
     5521 tatcactttc tattagaggt tatttttata cgaatgccat gattaaacat gataatacat
     5581 atagtataaa aggctcagtt gattttgaca atattaccat tgaaaatact ggtgttttgc
     5641 ctcattacat gattgtttcc aatgataatg tgagtattaa aagcagtatg aaaattaata
     5701 atgatttatc gcatgttatt attgattatg taagctttaa tgacaacgaa atagacatta
     5761 atgcttcatt acaattttta aaagataaat attttaacat cgctgttact accaatacta
     5821 ttgatctgga tgatattgat tatcttcaga taatacctga tgtttatggt gatgggacat
     5881 ttgcattgga tattcaatgt gattatgatt tatcgcataa tgaaatgaga tcttttaaaa
     5941 tatattgtga tgctgaagat atcgcaatct tcagtaaaac caaaaacaat gctatgacat
     6001 atattaagga ttcatggtta aaaattaatg gcgatataaa gaaaataaat attaatgcct
     6061 gcgctaaaca tcagaaatca gatgtgatgt tacgatcaac tattaatatt gagaagtggg
     6121 ttccttttac ctcaactgat gaaattatgg tacaatcaaa aagattgagt gctgaattgt
     6181 tatccaaact tttattgaaa ggcataaaag aattatactc caaagctgca gaagatttga
     6241 gtattggcta taacgaaatt tatttcaggc aaaaaccttt ggggaaatat ctatcaaaca
     6301 atataatcaa tacaaaaatc aatgtagata cattgacatt tgaaaagaat gctcatctga
     6361 aaaacattaa tatagagtta ctatctaaca aaggtttggt aaccactcaa tcattcaatt
     6421 gtgagggtta ttccgccaac tataatttta gtgttaatgc gttctgcaat agtgattatc
     6481 ctacaatgac aatttcatgt gcggtaaata attttgacta tggtacattt ttacgtgata
     6541 ctggtttgaa agatgcaaca ggtttaatga acatgaattt gaattatggt gtatcgggct
     6601 accgtttaag tcatctgttg caaaatggga acggcaattt tacattgaat atagcaaata
     6661 gcgttttaaa ttcatccatg attcaaagaa aaattataga gttagctaaa agctgtaata
     6721 tagttgttga gggaggaggg tggcattgta atcgaattga tgttgcaatc aatcatactg
     6781 cagacagatt aattttgcaa agtttgtata tcgatacaga taatgcttcc gtgagtgggt
     6841 atggaaacta ctcaattgaa aatggcctat cattgccatg taatgctact ttttataaaa
     6901 gagacggaag aacaattacc actactcata gagcatcatt ttcaataaaa ggtgagctgt
     6961 tacgtccttt tattgtatca tcttttccct gtggtaaaga acaaatatta ttgtttgatg
     7021 tcaattaatt catatatctt tacggggagt tgcttacctt taacacgtac taaatctagt
     7081 tctcgtgcta ttatataatc tttcacttgt tcataggtaa attcgctgat gatgatgttg
     7141 gtttgatatt gtttgtttgt tccttcaagg cgtgccccta aattgacgtt atcacccata
     7201 agggtgtaat ccattcgaga tgccgatccc atatttccta caaccatttc acctgtgttt
     7261 ataccaatac caatatgaag ggctggtttg ttcatctgaa tccagcgttt attcaattgt
     7321 tcaagtacct gtatcatttc aacggcacat ttacaagcca taagtgcatg gttgtcctgt
     7381 ggtataggcg ctccccagaa agccataatt tcatcaccaa catatttatc aagtgtgcca
     7441 tcatatttaa aaaccagttc tgtcattgcc tgtaaatatt cattaaggtg ttcaacaagc
     7501 tcttctggtg tcattttttc tgaaattgta gtaaaacttc gtatgtcaga aaaaagaaca
     7561 gtgaggaatt ttttttcacc acctaattta agattttcag gatttttaag aagttcatca
     7621 acaactgatt ttgatacaaa ttttgagaag gtctgtcgta tatatttctt ttcctgctgt
     7681 tcggttaaaa ctctgtaagc gataatgatt gaaaatgttg taccaacctg tataatagga
     7741 gatgcaaata ttgttatgat attaaacgca tcaaatagta aatatgaacc aataatatat
     7801 aacagtgata aacctacagt aaataagaca gaaattatga tagataccct ggtcaaaata
     7861 aatcctaata gtatggcaat tgcaaacaat ataagtatat tttggaaagg tgttagcttt
     7921 actataaagt cctgattaag gatggtattt aatgcattag catgatgctc aatgccaaaa
     7981 agatcgccat aaggagattt atggatatca gttgaaatac ctgttgacga atatgcagct
     8041 accaaaatta ttttatcctg taaagaggta ttggtaatta ttccatctcg gcagaaataa
     8101 taataaggat aatgggtaaa acttccagga cctccaataa agttgatgtc catgaaacct
     8161 tccgcatcaa tagggatttt tatgctgtta tcatgagttg ttttcaccat tttttctttt
     8221 ggtatgttcg atagttttat atattgtcct acttttattt ctacatcttt ttttgatatg
     8281 ccataatagt gcattaccag caagaggtca atagatggat aatacagacc tttatattta
     8341 acaaccagcg gaacctttct attaatatta tcagaatcag gttttatatt agcaaaaccc
     8401 agaccaattg cagcttgaga caatagagga gttgggggaa cagcttcttc tacccatggc
     8461 atggagttgt cattgggatc aattgtcatc gcgtaaatgt tcaacaggtt cattctttct
     8521 tctatatcag agtattttac atctacttct tctgtttcaa agggataatc taaaaaaaca
     8581 cagtttgcct gtttgatagc tttggcaaat tcatcttcat atggtttgtg atctaaaaac
     8641 attatatcaa aaaatatagc gaccggattg cctgatgcaa cgtattgagt aaattttgca
     8701 tgatataacc atgggaatgg ccatttaatt ccttcattgt caaattcacg tattgtattt
     8761 tcatcaattc caaggatgat tatatcatca cgtgaacggg gatttttgcg ggtcattttg
     8821 actccttcct gtagctttat tgctttttca gaaggatcac gcaaaaagaa gcgaaagttt
     8881 attgatccgt attcaagacc atcaagaagt ttggtttgtg cataaaaaaa tgagataatt
     8941 aaaaatactg caattgataa gcctaaacca aggaggtaac ttttattaat ttgcatgtta
     9001 tactaccttt gctatgaagt aacattaaag aaagtgtctc tatataagat agtatatacg
     9061 ggattgctgt aaataatgca agttttttat tatattcttg acaaaatatt tggtagataa
     9121 agaaaactaa ttaaaaatgg atgagaaata agattattct tatctatgct atgaatatat
     9181 gagtgaagtt atatcgattt tttagcggag gcatcacatt gataatcaaa gagtatattg
     9241 cacagattgt tgataaagct ttgcaaaagg tattacaggc acatgacata ttacaacaac
     9301 catcgctttc aatcaaaata gaatatccaa aagaagaaaa atttggtgat tatgctacgc
     9361 caattgcatt agaatgtgcc agaattttaa agacttcacc gctgcaaata ggagaacaga
     9421 taaaaggatt tattgatgaa cagcatattt ttgaacgcat cgatgtggta aaacctggct
     9481 ttttaaatat gtttttatca ttgccatttc tggttcagcg tttgcaggcg atagttactg
     9541 ctaatgatga ttatggtaga aataaaaaag aatatccgcg taaggtaaac attgaatttg
     9601 tatcagctaa tccaacaggt cctttaaatg tagtttcggc acgtgcagca gccgttggcg
     9661 atactctggc aaatttgctt gcagcaagtg gtgatacagt gaaccgggaa ttttatgtta
     9721 atgattatgg caatcaggta tggtggctgg gaaaatcagt gtgggttcgt taccagcaaa
     9781 tgctgggaaa ggatgtgata ttccctgacg aaggctacca tggtgaatat attaaggata
     9841 ttgcaaaata tatacatgaa aatttttcgc atgaactatc aaacataaac gatgaagaaa
     9901 aggctgtgca attttgtgcc cgcaaagctg tagattataa tattgcatgg caaaagcagg
     9961 accttgaacg gtttaatgta cattttgata catggtttta cgaatcaacc ttgcatgaaa
    10021 gtggcaaggt gctggatgtt tttaactatc ttgagtcgct tggtgttatt accgatgaca
    10081 atggtaaaaa ggtttttgtt tccacaaagt ttggtgatga taaagacagg gtggttattc
    10141 gcgatgatgg cagaccaacc tatttaatgg cagatattgc ctaccaccga acgaagattg
    10201 aacggggatt tgatctgatt attgatatat ggggtcccga tcaccatggc tatattgctc
    10261 gtcttgttgg agcgatgaag gcaatggggt atgatgaaaa tcaatttaga atattaatat
    10321 cacagcaggt taatttgcta accaacggtg aagcagtaaa gatgtcaaaa cggttgggaa
    10381 cattttcaac aatgcacgat cttattgatg atattggtac agatgttgca cgatattttt
    10441 ttgtaatgcg ttcaatggac agtcatctgg actttgatat tgatttagct aaacgtcaga
    10501 gcagtgaaaa tccggtcttt tacctgcaat acgctcatgc ccgtatatgc tcaatattca
    10561 gagaagcaca aaacagaaat atcaactatg ataaaataat tgttgatact gcgcattata
    10621 gtaatcctga aaccataaca ttattaaagc tgatggctcg ttttccagaa gaaatatatg
    10681 atgccgccaa tagttatgaa cctcatcgaa tcaccgtgta ttgtatgagg cttgctcaag
    10741 cataccataa gttttatact gaacacaggg ttctgtcaga tgaccatgat aggacacaaa
    10801 gctatctgat attatgtgat ggagtgcgta tcattttaaa aaatgctctt tcattattag
    10861 gggtctgtgc ccctgaacgt atgtaataaa aataaaataa tttattgatt ttttgttata
    10921 acattggttg aattacacca aactaatcta tagtaataga ataattataa tgacaaaagt
    10981 tgctttgatt gctacaggca gtgaattggt caatggccgt attcatgaaa gtaatagtta
    11041 ctatattagc aggcaattgt tttctatagg gttacctgtt gttgctcatt atgttgtggg
    11101 cgatgatagt gcatccttgc ggtcaattat tgaacatgct atgtatagtg ctgatatcgt
    11161 cttcataaca ggtggattgg gtcctacggt tgatgatata acctgtgaaa ctttgcagag
    11221 catgtttcat ctgaatttga ctgtacacaa accctcaaaa gcaaggatgg attatttctt
    11281 taaagccact ggcaagtctg ctcttgatac cgatgtaaag atggtaacag taccggaaaa
    11341 tgcttatgtt tttccaaatg agaatgggct tgcgcctggt tttgctattg ggtatgacaa
    11401 taaagatatt attgtactgc ctggagtacc tcacgagttg cagtcaatga tgatgagtgt
    11461 tatagaatat tgtaagaaaa aatatcaatt acaaaaaaag gaagagctta cctttaaagt
    11521 tattatgatg cgtgaagcgg aggttgattc ggctgttaaa gaaattgtaa ataaacagga
    11581 aaatattgat attgcaataa cctatgaatc aggtacaacg acagttatac tgagtcctaa
    11641 aagtggcaat gtattacctg ccgttgatat tgttgatgaa ataaaaaaaa ggtttggttc
    11701 aaaactgatt tttggatata attcactgga agaaaaagtt gtacatttat tggcaaaaaa
    11761 taaattaaga gtagctgtcg ccgaatcatg tactggaggg cttatatcca aacggattac
    11821 cgatattcca ggtgcatcca gcatatttgc aggtggtgta attgcctata gcaatgaagc
    11881 aaagaaaact ctattgggtg ttaaagtaga tacattgaat aggtttggtg ctgtaagcag
    11941 agaaacagca ctggaaatgg catggggagt taaacaaagg tttgcatctg attttggtat
    12001 tgcaacaacg ggaatagccg gaccggatgg tgggacaaag gaaaaaccgg taggtctggt
    12061 atgttttggt atagcaggta gcgatacttt ttcaacacaa atccagttta gtggtaatag
    12121 ggagcatgtc agaacgttag cagccaatta tattttaaat aaactgttaa catttgcggg
    12181 gattgaataa gaatgaaaag acgggtaatt gtacttgtta ttttactgca cagtatagtg
    12241 tgttcactgg cttttgccct tgaaagctcc caactgtttg aacagggtat ggaagctttc
    12301 aggatgggga attatgggtc ttcggagtta atcttcagaa aaattattga tgccaatgat
    12361 tcatataagg atagagcatg gtattattta gcattatcga ttttctatca gaaacaatat
    12421 cgttctgcta tatttgagtt gaaccgattt cttcttttgt gtacaacccc tgatttatgt
    12481 aatgaagccc gttactggat tgctgaatca tattactatt taaatgatta tataaaagcc
    12541 attgaagagt ataacagatt tattactcaa aataaaaata agcagcttat tataaattca
    12601 tatgatcgta taggtgagat atattttaat caaaaaaggt atgatgaagc cataattgaa
    12661 tggagaaaag cattaacatt aaccagtaaa aagtttgaag atagtaacag ggtgataaaa
    12721 attggtgaag ctctttttct tagtgaacgt tattcagaag cactggatat tcttgaacca
    12781 ttaattcatg cggtgaatga tgcaactatt tcatcaaaag cacagcttat cgttggaagg
    12841 atttatcaga tacagggtaa gcattggcag gcaattaaat atttaaatga aattcccgaa
    12901 tcaatgatta aaatacctgc ttttttaagt gtacagtatt ttaaagcttt atcatatatt
    12961 gcactgaatg atatctataa tgcacggtta aatcttaaat cgtatatttt aatttctaat
    13021 gaagattcac cctattatta taatgcacgg tatgaattag gaaaaatatt gctaaataca
    13081 aaggatttag atgaaggcat atcactttta gctgatgtgc aggtaaacag caatgatatc
    13141 gatttgagga gcagggcttc atttgaatta ggacaattgt atttaaaaaa taatacaatc
    13201 gaaaaagcaa ttccttatct tgaaaatgct atttcaatta atgatcagga aagtcaaaag
    13261 tacgttatga tgacactgag cgaattatat atcgctacag atcgctatga tgatgccaaa
    13321 cgtttacttg attttatgct ggaaaaatat ccttatgaag aaaataggga tgaaatgctc
    13381 tatcttgaag caaaaatata tattttgcag ggagatgttg aaaagtcact tgaatatttt
    13441 gagctaatta aagaagttaa tccattttca aaatatcttg ttgagcttga ttatgcccag
    13501 gctgttgatt tttttaaacg cggtgattat accagtgcaa ttgcaagctg taacagatat
    13561 ttaaataatt ataaagttga taacagatat gaagttttaa taatattatc gagatcatat
    13621 ataaaaatta ataattttaa aaaagcagtt gagacaataa accagttaat agcaaagttc
    13681 cccaaaaaaa acgggttgga cgagatttta tatgagttgt cgctatcatt aaatgagaag
    13741 ggggtaaatg ttgagaaata ctggaacttc attattaaaa attactattc gtctattact
    13801 gccggtaaga tatatataga aaaaggagac gaagcatata aaaagaaaaa ttatcagagt
    13861 gcttcatatt tttactcaca atatctatca ataaaaggaa gaccaagaga gcgttcggtt
    13921 ttactgtttt atatcatttc tttatatgag ttgaaacagt acaacacaat aattaagacg
    13981 ctgaaggata gtgaatatat agctgttgat gattatacaa caaaattatt gattaaatgg
    14041 ttagcaaaga gttattatat gctgaaggat tacaccattg cattaaacac gtttatgaaa
    14101 ctaccactca tggattatag tatggatgat ttagttttga tttctgatac agcattaaag
    14161 aataatcaac ctgatattgc tattgaaaca ttacagtata tggagcagga caatcggtat
    14221 aatgaagttg cttacaatat tgctttatac tataagaaca atggcaatat tgatttagca
    14281 accgaatatt tcaaaaaggt cttaataaag aaacaccatg gcatggtgtt gagcaatgca
    14341 caaatacaat tagctcagat ttatttagag caggaacagt ttgataatgc gctggaatta
    14401 gtaaaaaata tacaggataa agaaaatata attatgcgtg atagtatagc attcatagca
    14461 cattataaac ttgggaatat aaatgaagct ttaagcatta ttaacaataa attaaatgat
    14521 ataaatatga ccgcttttgc agatgaaata tttactttgt tactgcaaca ttattataaa
    14581 cagaatgata taaaaaattt aaagattatt tattcatata gcagtaaacg aaaaaaactt
    14641 atttcagttt ctaattatta ttatggtttg atacattata ttaataataa ttataatcag
    14701 gctattaatt attttactat tgcttcacgc tttcaggatg aatatggtat atcttcacga
    14761 tattatctag ggatcattta ttttgcgata caaaagaatt ttgttaccgc aaaaagaaat
    14821 tttgatcgct attttgaaaa agctgaacag gatgatataa actattataa tgctaagatt
    14881 tatgtatcaa tgcttctttt tgaaacaggg aaaaaagatg agtcaaagac tattctttat
    14941 gagatagtta ataatgaggg aaatattctt gcaaaaataa aggcaactca tcttattaaa
    15001 cattatggtt tttgatgtgt gttcgataat gatagtatat gtataatttg catgatttgc
    15061 gtttctacga ggaatatatg aaacaataca ctttccatgt agttcatggt ccaaacttaa
    15121 atatgcttgg gacaagagag cctggtatat atggtgtttt tacacttgat gaaatcaata
    15181 ataaaattat tgaatatgca aagtcgcata atataaccat acattgtttt cagacaaaca
    15241 gtgaaggtga aattatagat tatattcaaa gaaataaaaa ttgtgatggc attatgataa
    15301 atccaggtgg gtatacgcat acatccgttg caattcgtga ttgtatagcc ggatgtaata
    15361 caccggtcat agaggttcat ctatcaaata tacatgcacg ggaacatttc cgaaaacatt
    15421 cgtacatagc acctgtttgc attgggcaga tatgcggatt tggttattac tcatatatat
    15481 tgggaataga agcactggta tattatctaa acaatattca aaaataatgt taatagtatg
    15541 aactatctgt ccagaataaa aaagataaga aacaaattaa aaaacaatga ctgttttcct
    15601 tatctggtaa gtagtcttat caatatcaaa tactgcactg gatttgatgg gagttacgga
    15661 tatttaattt tttttcctga tgaaactgtt tttatatctg attcgagata tgaagaatat
    15721 gctatttcga ttttgcctca aaacgtgcgc tttgtgttac aggaaaaaga tataaatcaa
    15781 acactatcgg cattgttaaa aaagaaaagc gttaaaaagc tctttttgga agaacatgct
    15841 atttctttat cgcaatataa ggcaatgaaa gaacattgca gagggattgc actagaatat
    15901 ggtggagata gtatcaatga attgcgtgtt ataaaagaag aggaagagat tgaaattata
    15961 aagcaagctg caaaacttac tgatttatgt tttaatcata ttttaaatat acttaaacct
    16021 ggaattactg aatgggatgt tgcaacagaa atagaatatt tttataaaaa aaatggttgt
    16081 ttaggaacat catttgcaac tattgtagca tctggtaatg gttcttcaat gcctcattat
    16141 aagactgatg taaaaataat taaagaaggt gatcccgtcc ttattgatat gggatgtatt
    16201 tacaatggat attgttctga tattacacgg acggtttttg ttcatagtgc ttcagaagat
    16261 ataaaaaata tttacgtaat agtaaaaaaa gctcaagaga atgccattgc agcagtaaaa
    16321 ccaggaatga ctacaaataa acttgacagt attgcacggg attttatagc tgctcatgga
    16381 tatgcagata agtttgggca ttcacttggt catggagtag gaatggatgt tcatgagatt
    16441 ccggccatta agcaaaatgg catgatcaaa ttaaaaaagg ggatggtgat aaccatcgag
    16501 cctggtatct atttaccaaa acgtggtgga gtacgtattg aagacatggt tttaataaca
    16561 aacaatggat atgaggtatt aacaaagagc ccaaaagata taatagttct ttaatatggt
    16621 gcatagcagt gttttgtata attatttttt gtggtaaaaa gcatacgcaa atgtaaacat
    16681 agtgaatgag ttgttgatgg gcgggaataa gggatgaaaa tatttgttgg cagtcatact
    16741 gaatcatatc atggactgat ttcaggatat aacgatacct attgggattc ctgaacaggt
    16801 ccggaatgac attgaggtgt aattctattt tcggaggagt tgtaatgata acaagtaatg
    16861 atttacgaaa cggaacagta atcaaagttg atggtgattt atattctgtt gttgattatc
    16921 agcatgtaaa attgggtcgt ggcggtgcgt ttgtacaaac aaagttgaaa tccattacaa
    16981 aaggtactat tgttgataag aagtttaaag gtggcgaaaa ggttgaagat gtaagaatag
    17041 agaaaaagac tttacagttt ttatatgatg aaggtgatgc gctgattttt atggacacgg
    17101 aaacatatga tcagttaagt attgataaaa agaaggcagg gaacctgctt aattatatta
    17161 aagaaggaga taatgttgaa gcaacgatgc ataatgatgc tatattgtca attgaaccgc
    17221 caatatttgt agttttaaaa gtgacctatg ctgaacccgg ggtaaaaggt gatactgcaa
    17281 ccaatgtgtt gaaaccggtg actgttgaga ccggggcaac tatacaggtt ccaatatttg
    17341 taaatgaagg cgatactatt aaagttgata cacgaaaagg tgagtacgtg gaaagggtta
    17401 aaagataact attgtagcat aaatagttat actctataaa aatttattat taagaactac
    17461 aatgtgaaat caaatataat aacgaaagaa gcatataaag catttaaaaa aggggaaact
    17521 tccgttgtta tacaactatt agaaaaaaat attgctatct caaatgatcc ctatgatttt
    17581 tttttgcttg taatagcata tctattaaca gataaactgg tgcaagccga agcaacatta
    17641 cggaaagcac tccgacaaca tcaaaactat attcctcttt tagagattaa ggcatttctt
    17701 atattgaaag ctgcaaaaac aaaagatgat gcctgtagcg gatatgtaga tatcattaaa
    17761 ctatcgccca acaataaaaa aattacaaag attattaaaa ccgtacatga aactgataat
    17821 tttgaaaaat ttcaaaagca agctaggatt aaagatttta tcaccataca accaccaaaa
    17881 aaacatgcaa tgcacaaaca ccagcattct gggcgtgcaa gatttccagt aatgaaatgt
    17941 gtactgcttt ttagcattat agctataatc attgcaggaa ttattttttt ctcatcaccg
    18001 ctggcaaaac ttataaccca aaaaatttcg ttacttttta ctgaaacacg gtcagatata
    18061 catgaactat caaacataac acttgatggt atgcattatg atgttataga taccatacaa
    18121 aaatttaaaa cacctgaatt ttatacttcc ggagaagagt gtatacgtga ttttaatcat
    18181 gctaagtggc tgatcaaaga aggtaaagaa aatgacgcaa tgattattct gaataaaata
    18241 gatgcaagta atgttcagat gaaggttaaa caacgtgttg catttttaaa ggatttcatc
    18301 attagaaata gagaaaggac agtttcaggt tacttgtata atgatgttat aaagaaaccg
    18361 tatctatata aggggataaa tgttaaatgg cgtggtaaag tagcaaatta taaaaaacaa
    18421 ggcaggaatc atacatttca attattaata aattatgaaa atgacagatt tgatggtgtg
    18481 gttgaagttt tttcatctac tgctgataac agtataacca atggcacaat agctaatgtt
    18541 gaaggaatta ttaccaatat agttgctcag aaaattatag tactacatgc gttaaatatt
    18601 gaaattgtcc gttaacagtt gtatatcgta tagtgtattc ataaggaaga aatatggaag
    18661 ttattttaaa agaatgtaaa acctataatg atgatattgt tacaaaacaa attaccagtg
    18721 cattgaatgc attgcatttt gatttttcac ggttttataa tgcaaaggtt gctatcaagc
    18781 caaatctttt aacatcaact gcacctgaag cagcagttgt tacgcatcct ttatttttca
    18841 gggcgatagt tcatgttatt aagcaacacg gtggaatacc catccttgtt gagtcaccag
    18901 ccgtacattc attgcagcgt gtgatgaaaa aagcaggtta tgacgcgata gttgctgatg
    18961 aggagattca ggttgctgat acttcagatt atattatcat acataatgaa aatgcacggc
    19021 attttaaata ttttgaagtt ccaaaaatat ttacagaatg tgatatcatc atcaatcttc
    19081 ccaaatttaa gactcatgcg ttaacctata ttacatgcgc tgtgaaaaat ctttttggaa
    19141 caatccatgg tatgaaaaaa tcccaatggc atattaaggc aaaaacaaaa tccgaatttt
    19201 ccgagatgtt gttggattta tatgaagcat atattactga tagttcattg ccaaaaacta
    19261 tcatacatat tgttgatgca attaccattc tggaaggaga tggtccgggg cttaaaggta
    19321 acccaaaatt tatgggaatc attggagcat catataatgc tatagccgtt gattatgcac
    19381 tttcaaagat tgctggtttt actattgatg aaattcctac cattacaatg ggtatgaaga
    19441 ggaatatatg tgctgtgagt gatgcaatta ccatagtcaa agatgcagct attgaaaata
    19501 actacaactg catacctcca aaagaatcag gttcacaatc aatactatct atctcaattg
    19561 taaatagatt attgaaaaat ttgattattg ctaagccatt acctgatgat aataaatgca
    19621 ctctgtgtta tcaatgtaaa cagatatgcc ctgctaaagc aatcagtatt gcaaaagatg
    19681 gagaaacaat tcccaattat aattattcaa aatgcatacg atgctattgt tgtatggaga
    19741 tatgtcctga gtcggcaata tcattgtcac aaccatggtt acaaaaattg atgaggtaaa
    19801 atagtattat gaaaaataaa tacacattaa aaaaaatagt tataattgaa ttcattgtct
    19861 ttttgttact ggttatgttt ttttggtttg aagagatact tgatttgcca tatattattt
    19921 ttaatgaaga gcccacgcca ataaattttg tggaatgctt aatggaaacg gttgttataa
    19981 caatactttt tggcatttta ttatatgaaa caataaaaat taatctttca atgggaaagt
    20041 tagagtcata tatctatgta tgtgctcgtt gtcacaaaat ttttattaat ggtgaatgga
    20101 tttcactgga caaatatttt aatgaatatg caaacaaaaa aacatcgcat ggattatgtg
    20161 atgtttgtaa aagaaattac actaaagaag attagcggag gagggactcg aaccccccac
    20221 accacggata tgagccgtgt gctctaacct actgagctac tccgccttat tagcgcgggc
    20281 aggatttgaa cctgcgacct ttgggttatg agcccaacga gctaccagct gctccaccgc
    20341 gcggtcattt aacatgatgt gacaatctat ttataatata cattgtagtc aatcattttt
    20401 caaattcttt attaaacctt atactttatt attaattttt attaaatttt ttttgaatgt
    20461 tgcaatatta ttgttgaaat ttttgaaggt gatataatac tgacaatgtt ctgtttgagc
    20521 agagggggtg atattatttt tagctaaaat ctgaagaaga ttttagcata tcatgtaagg
    20581 taaagcgcaa agaacccaaa ggaacacaat ccccagggtg gcgaatttaa gttaacctta
    20641 aaaaccattc ttaatgcata cactcaccgt agtgtaggtg attttataat tatgaggtgg
    20701 ttgcccatgg agggtttagg tacacacatt attgcggagc tcttcaactg caacgcgttc
    20761 cacatcaaca atttgaaaaa ggttgaggag gtattgacgg ccgcagccga actctctaaa
    20821 gccactatca tccaaccatt ttttcataag ttttctcctt acggtattag cggagtaatc
    20881 gtcatagccg agtcgcatat cacaatccac acatggccgg agtatgggta tgctgctgtt
    20941 gacgtcttca cctgcggaga tcatgactat aaaaatgcta tcgattatat cactgaaaaa
    21001 ctggaagcag atcgctgtgt tctgtatgag atgcgtagag gtgtgatgaa cgatagtaag
    21061 gttaaatgta ttccagtaat tcgggaggta gaaaatgtta actacattga atagcgagat
    21121 tcaaataaaa attccgacca ataatcaatt tgaacaatta ggtgcatggg gattatattg
    21181 tagtgtcgat atccataaat gtgatccgca tattattcgt gatgcggaga tgatacggca
    21241 gtatgctatt cagttatgta accttattga aatgaaacga tttggagaaa ctcaagttgt
    21301 taattttggt gaagatgagc gtgttgctgg attttcaatg gtacagctta tagaaacatc
    21361 aatgatatca ggacactttg caaatcttac caatacagct tatattgata tatttagctg
    21421 taaatattac gatcctgaaa tagctgctca gttttcacta agttatttca aaggtagtga
    21481 ctatacattg aatgtagcat tgagaaagta acatagatga taaaaaggtg gatggatttc
    21541 catccaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gaatcaaatg
    21661 tttggattga tgaaatatat gaagtagaaa gtggaagaac tatgaaatta aaggttattg
    21721 ataaattagt aacctatgat tcaaaatttc aaaagattga aatatatgaa acaaaagcat
    21781 ttgggaggtt gttggtgctt gatggagtat tcatgacaac cgaacttgat gagatgggtt
    21841 atcatgaaat gatagcccat gttcccatgt gtgtacatcc agctcctaaa cgtgttcttg
    21901 tgataggtgg tggagatggt ggtacattgc gagaagtatt aaagcataaa gaagtagaaa
    21961 aagctgatat atgcgaaatt gatggtgatg ttatcagatt gtgcaaagaa tatatgcctg
    22021 ctttatcctg ttcatttgat gatccaaggg ttaccgttta tactgaagat ggtgcaaaat
    22081 tcattgcaga aagaaaaaat gcttatgata taattcttgt agattcatcc gacccaatag
    22141 gtccagcagc agtgttattt tcagaacaat tttataaaaa tgtaagccaa tgtttggatt
    22201 cagatggtat tgcatccact caatctgaat caatatttta tcacagcaat atcattgtga
    22261 ccttagcaaa ctataataaa aaatatttta aggtgccatc atactattac accatggttc
    22321 caacttatcc aagcggtatg attggttttt cattttgttc aaagaaatat cacccgctca
    22381 atgattttat acaagaaaga gcacaggtgc tattgccaca attacaatat tataatcctg
    22441 atattcatag aggggctttt gcgttaccgc agttcattaa atcaaaatta accgggatta
    22501 tagtgtaagt tccaaagtat tttattaatt aaaatatgga gtactattat ttataacagc
    22561 caaacaaatt gggaggatgg tgaagcatta gatagtaata gtatttacta tcagcattga
    22621 tttatcttcc tgttcaggtt caactcggta aagaatacct tcgggtgatg caataaggct
    22681 tcgtgcctgt aaaaaatttt tataatcgga ttttactccg cataccttaa ctataattgc
    22741 atcagctaat tttgctcccc gtatgaatat atcattatca cgtttatatt tttcgtcgat
    22801 tgtttcatta cggaatagtg aaaatgttgg aataaatatt atttgcaccc cctgctcttt
    22861 aagagcaata aaattttcat ccttaaaaac atctgcacat ataagaacac tgaatcgtat
    22921 gccatacgca ttaaatataa catgaccatt gcccggagta attttccctt cttcggcaaa
    22981 aaaaaggttt tgtttacgat aaaaacccag tattgtccca ttgtgaaaaa caaaggttgt
    23041 gttgtgcaat ttatttccat ttaattcggg cattgtaccg ccaatgacaa ctgtgtcaag
    23101 ctcacgtgat aatagctgta tgcgcttcaa ttgtaattgt tgattgtgat aagtctgggc
    23161 atggttacct aattttttgt ttacaaagaa atattcagga aaacaaatga agtgcggttt
    23221 tattctctgt aaattttgaa aggtgtgatg cgtgatgaaa cttcctaatg gatattgata
    23281 caatacgatt gtaaaatcca tagacaatcc tttgatacat aatgagaaac tgtttgtaag
    23341 tcaatatatc aattaataat aactatctca taacaatata tcatttattt ggtgagatgt
    23401 taaaaaaatg tgtgatagtt atttctaaaa actaataaaa ttatcagtat cattattaaa
    23461 atcttgactt gtttttactg agaaactact attgcccgat tactatttat tataggtgga
    23521 gactatggga actatacaaa cattcagggt atatccatat ttacctgata atcttaaacc
    23581 attaaaaaca attgcatata atttgtggtg ggtgtggaat tttgaagcta tagagctttt
    23641 cagacgtcta aatgttgaac tctggaggga tttagaccat aatcccgtag ctcttcttgg
    23701 tatgctttca cagtccgaat tggacgaagc agcagattca gaaagtttta tggcacattt
    23761 aaaacgagtt tctgaagagc ttgagtggca tttaacaaaa aaatcatggg ttgaagatgc
    23821 atatcctacc tataaaaata tgcagattgc ttattttagt gctgaatttg gaattcatga
    23881 atgtttgcca atttattcag gcggtttggg agttttagct ggtgatcata tcaagtcggc
    23941 aagtgaatta ggacttccgt tttatgctgt tgggctattg tatcgtctgg gatatttcca
    24001 ccagtatctc aatatcgacg gctggcagca ggaaagatat cctgagaccg actttcacaa
    24061 tttgccatta caactgattt taaaagacga taagcctttg gtagttgaag ttgaatatcc
    24121 cggcaggatt gtatttgcac agatatggga atgtaatatt ggccgtgtta aactttacct
    24181 tttagataca aatataacgc aaaataatct ggaagaccgt actataactg accagcttta
    24241 tggtggtgat gttgaaatgc gtattaaaca ggaaatgttg ttggggatag gtggatacag
    24301 attattaaaa gcattgggga aaaatatatc cgtttatcat atgaatgaag gacattcagc
    24361 ttttttaagc attgagcgga ttcatgatac tatgcataca cataaattaa acttttatga
    24421 agccattgaa tatgttaatg ccagcactgt ttttaccaca catacccctg ttcctgcagg
    24481 taatgataga tttgttccag aacttattga taaatatcta tcgcattatt ttcaaaaatt
    24541 aagcatatct cgtgatgagt ttttggcatt agggagagaa aatcctcatg ataaaataga
    24601 aacattttgt atgacagttt tagcaattaa aactgctgct cattgcaacg gggtatctaa
    24661 attgcatggt tcggtatcac gtagaatgtg gaaaaatata tggccagggg tgccggttaa
    24721 tgaggtgcca atttatcatg ttaccaatgg cgtccagatt ctttcatgga catctgatga
    24781 aatgatgagg ttattgaaca gataccttgg tcctagatgg attgataatc cggtagataa
    24841 aacaatatgg aaaggtattg acaccattcc tgattctgag ctatggcgct gtagagagcg
    24901 tttgcgtgaa cgggtagtgg catttgcacg taaaaagtta cgggaacaat tgattgcacg
    24961 aggtgcacca agggcagaga ttgagaaagc agattcagta cttgactccg aggcattgac
    25021 aataggattt gcacggcgat ttgcgaccta taaaagggct acacttatac taaaagatat
    25081 gcaacgtttg gaaagaatac tattaaataa ggaaaaacca gtacagatta tattttcagg
    25141 gaaggcacat cctcgggata atatgggtaa agaattaatt aagcggatta tccatttagg
    25201 cagggatgat cggttcagga gcaaaattgt ttttcttgaa gattatgata tcaatattgc
    25261 tcgatatatt gtacagggcg ttgatgtatg gctcaataca ccaatacggc ctcaggaggc
    25321 atcgggaaca agcggcatga aggttgttcc taatggtgca ctcaatataa gtgtattgga
    25381 tggctggtgg ccagaagcat tcaacggtaa taatggatgg gcaattggaa agggtgaaga
    25441 ataccatgac cctgattatc aagatgaggt tgaaagctta tcattataca atattctgga
    25501 acaagaagtt attccacttt tttacaacag gggcgcagat ggtatccctc gtgaatgggc
    25561 aaatcgtatt aaagaatcaa tgaaaacaat attgccgcaa tttaacacaa atagaatggt
    25621 gaaagagtat actgaaatgt actatcttac agcacatgat aactttgtga agttcagtaa
    25681 ggatgatttt aaagcatctc gttcactggc taagtggaaa aaagatataa tgcaaaaatg
    25741 gcatcaggtt actcttgaat ctattaccta tgaaagtaaa aaagagatta ttgttggttc
    25801 tcaactgaaa gcaaaagctg tagtcaattt gggtgatctg aaaccaactg atgttcaggt
    25861 agaattatat tttggaaact taaatcagaa aggcgaaatt gaggatgggg ctgcattgcc
    25921 tatgcatatg gtagaagaat cgggtggtgg aaaatatatc tatgagggaa ggatgctctg
    25981 ccttaaatcc ggacaatttg gatttaccgt aagggttatt cccttacatt cagatatgcc
    26041 aagaaaatat gaatcgcgac ttattacctg ggcgtaatag ggtttattct tttattattc
    26101 tatctttaag atagttaaaa aagcttcttg aggtatttca accgaaccaa tttgtttaag
    26161 gcgttttttg ccttctttct gtttttcaag aagttttcgt ttacgggtaa tgtctccccc
    26221 atagcatttt gctgtaacat tctttcttaa tgatgatata ttttctcggg caataatgcg
    26281 agagcctatc gctgcctgta atggtatttt aaattgttgt cgcgggataa cctctttcaa
    26341 cctttcaata atctcacggc ctcgcttata tgctttatct ccatgaacca tgaatgaaag
    26401 agcatcaact gattcttcat ttaccagaat atcaacctta acaatatctg aagctctata
    26461 atcgataagt tcataatcaa atgaagcata tccacgagtg cacgatttta gcttatcgta
    26521 aaaattgaac ataatctcgg caagaggcat ttcgtaaaac agttctactc tagttggatc
    26581 aatatattcc atttttttgt gtattccgcg gcattcatta accagagcca tgatattgcc
    26641 aataaattca gatggagtaa tgatagtagc ctgtacaaac ggttcttcta tacgttctat
    26701 ttgtgatggg tcgggcatct taactggatt atcaattaat agaacttcgc ctgttgtttt
    26761 tataacgcgg tattctacgg taggagcagt tgtaataagg gctaaattaa attcgcgttc
    26821 caggcgttcc tgaacgattt ccatgtgaag caaaccaagg taaccacacc tgaaaccaaa
    26881 gcctaacgca tacgagttat caggttcaaa gtgtaatgct gcatcattaa gctttaattt
    26941 gtacaatgca tcttttagat catcatagtc atcgcttatt gatggataca gtccggaaaa
    27001 taccattggt tttgcttctt taaatcctat gattgggtgt gaaacaggat tttctgcgga
    27061 tgttatagta tcaccggtac gggtatcggt tagctgtttt atccctgctg ttatgtatcc
    27121 tacttcaccg gcggttagct gtactcgttt ttctcgcttt agccgaaaat gtcctacctc
    27181 ggtaacctca tactgctttt gtgttgaaaa tagcataatt tcatcatctt cttgaacctg
    27241 cccatcaaat attcgtactt tggtaacaac accaacatat tgatcaaaat aggagtcaaa
    27301 aattaaagcc ttaagtggtg cagaagggtt gccttttggt gcagggatta tttcgatgat
    27361 tttttccaga agttcatcta taccaattcc ttcgcgtgct gaaacacata atactgtatc
    27421 aggatcaagt cctaaacttt ttttaatgct ttctttagta cgctggatgt cagcatttgg
    27481 catgtctatt ttattaatga ccggcaggat ggtaaggtca ttttctagag ctaagtacat
    27541 attagcaatt gtttgagctt caactccctg agtggcatct acaagcagca atactccttc
    27601 acatgcggct aatgctctgg atacctcata ggcaaaatca acatgaccag gagtgtctat
    27661 cagattaaat gtatatattt ttccatcttt cgactgatag ttcagtgtaa tgacgttggc
    27721 ttttatggta attccacgtt cacgctcaat atccatatca tccagaagct gatcaacgtg
    27781 attgcgtggg tcgatagctt tacaacgctc aataatacga tcagccaatg ttgatttgcc
    27841 atgatcaata tgtgcaatga tggaaaaatt tctgataagt gattgttcca tataatcaaa
    27901 acctaccgtg ttattgatac aataaactat agcgtgtgtt ataaaaaaag cagactgttt
    27961 atataaatat ataaaattta ttcttgatga actatcgcca atccgattta aataatgcaa
    28021 ctacttttta ttgagtgtaa tttatgaaag tactggtaat ttcggatact catggaaata
    28081 ttgatgcaat aaaaactata atagaagctg aaaggaagtt tgattatcta ttacattgcg
    28141 gtgatggcat caatgacctg atgtatgtga gtgtaccgga aggtgtgttt gtgataacag
    28201 tatgcggcaa catcgatagg gcaaggggca tcaatgaaaa aagcacaatt gttgaaacaa
    28261 taggcaattt taaatttttt ataacccacg gggatgtata tggagcccat catgacatta
    28321 caggagtttg gattgaagct aaacgttcag gatgtgatat agcgtgtttt ggccatactc
    28381 ataagccttg caatactaat gggagtccaa tgatgtttaa tcccggagcg gcacaaagtg
    28441 gtttttatgg aattatagaa agtggtgatt cattaaaagc tgtattaaaa aggatataaa
    28501 ggatacaaag gatataaaag aatatacaat gtttagagaa gggtctcttt gatctgcaaa
    28561 aatctattgt gatgttttat aaaagcttcc acaattacag gatcaaaata tgttcccgct
    28621 tttttagaaa taatttcaac tgccttctca tgaggaaaag cacgtttata ggggcgttct
    28681 gaaacaagag catcatatac atcagctata gcaactatgc gtgctgaaaa aggtatagca
    28741 tcacctttca aacctttggg gtatcctgag ccatcccatc gttcatgatg ataataggca
    28801 atttctttcc ctaaattgag ataggaacgt ccttcaacct ttttttctgc ttccgctata
    28861 gtttcagcac ctattattgt atgtgttttc attatctcat attcatagtc ggtaagtttt
    28921 ccaggtttaa gcaaaatatg atcaggaact ccaaccttcc caatatcatg taaaagacat
    28981 gaatttacca gatcctgtat atattcttcg gttatatagt ctctataatc aggatgttgt
    29041 gatagctccc gtgctaaaat ttcaacatag cgcatgatac gttcaagatg catgccagtt
    29101 tctttgttac gatattctgc caactttgcc agtgatacta tggtaaccag ctgggcatgc
    29161 tggagttttt tatttagctc taatgattca gcttcaagtt gcttcttctc actgatgtca
    29221 ataaaaaagt gaagacgtgc aaatggcatg tcatgatcat catgtaaaag agatgacatg
    29281 cacagggtgg taataatagt gccacctttt ttaagcatct taatctctgt atacggttgg
    29341 tttatgattg aggcaaaagt atttgatttt gacaaaactg aatcttcata ataattaaga
    29401 ataagatttt gcaagggagt attgagtgtt tcaaagggct gatattgcag tatacttgtt
    29461 gctgccttat tccagctggt aatgataccg ctcagatcag tagttataat tccaatcatc
    29521 tcattttgta atatcttttc ctgaaattct gttaggtttt gtatatgtga ttccattctt
    29581 tttttacgtt caatatcacg agcaacaatc tgaataccca cagtatgatc aagtggtatc
    29641 atgctcatgg caatttcaaa ccataaaaat ttttcatcac aggtatacat ctgaatctca
    29701 aatgggtcag gattaattcc ataattaagc aaatgttgca ttattgattg tattaaatat
    29761 ttttcattat catcaatgaa attaaaaaat gaaggttttc tggtttcggg atatgccagc
    29821 agtttataaa aagccgagtt gccgtaaatg atttcacctt ctgcagtgca cagcattatt
    29881 atatcctgag tattttctac cagattacga tattttaatt ccgattttac aagctcttcc
    29941 tgatttcgtt tgcggtttat ttcaaccgct gcagcattga gtaatatctc tatataatct
    30001 ttgttttctt caattgactg tttgtctaaa gcaattgcta tacttccttc tatctggtgt
    30061 tttgatccaa gaggataaat gccaattact tcatctttta gtaatgaact aatttgtttt
    30121 atcttatcgc tttcaatctg tggaaatgtt gattcaagga atgatatcaa ttcagaaaaa
    30181 tgttctatca ttatttcttt accgctttta ataaccgaaa aaaacggtat atttgctgaa
    30241 tcatcaaaat caaatattat agtggtaata ttttgattgg taaccgcttg aagcaattgc
    30301 aaaaagctgg caggatatac ttcaactaat tgaagtgtat tgacatcact gctgagcaaa
    30361 gttacaatac aatatttaca acccaatgta tgcagcgatt caataataca gtgtatactt
    30421 tcttttatgg tgttagcctg tttcaatgca tacaacgtgt tggtggttaa ctgcaatttt
    30481 tgctgttttt cctttaattg ttttgtttga ttgtctactt cttctgctaa tctcaggcta
    30541 aatttttcta tttttttctc ggcattgata cgtgttgtta tgtcctccat aataacaact
    30601 actgaagaga tgctgcccga tggtgcaatg atgggggaag taatatagtt aaaaaaacgt
    30661 gtatccttaa aataaggaac ttttttttcc tgtagtatgg gagctgcttt gtccattgct
    30721 tttgaggcag ggcagccatt acaggtttta ttgctacaac caactatctc ataacatttt
    30781 ttattgagtg ggcgctctat gttaaaccat tttttaaagg tatcgttagt ttctataata
    30841 gaatactctt tgttaatgac aaataatgcc atagggatag cattgacaag cgaagaatat
    30901 tttgtttcag aaataataat tttattctgg taacgatatt gccgcaaccc actggctatt
    30961 aattttgcaa taagttcaag ggttgcaaat tcattgtcat ttatttcagg atagtttttt
    31021 ggataaacaa catcaaacga gcctatgaca tctctatcgg aaaaaagtgg aaatgatata
    31081 gcgctgccaa aaagaggtga ctccagaata cgattctgaa catcaggatt gagtatctga
    31141 aataattcct tacttgcttg agaaaaatcg cttatcattt gttcacatga aacaattgct
    31201 ttatgatact gtattacctc aggtatggaa atagtatcgg tcagtgttaa ataaccgcgt
    31261 aatagtgaat gcaatatact ttttttaaaa atagcacgat tggcaattaa ctttttaatg
    31321 ttgatggtgg catattcaaa gatattgttt gatatggata gctcaggcac ccattgctta
    31381 agggaatccc aatcgggaaa tattaataga tgatcggtgc cttcgatgtt tgaaaggcag
    31441 gaaatattaa aaacaggaat ttttagctgg gcgatagttg tttttagatt gtgaacatac
    31501 tctgataggg aagacgtagt atcaagattt gcaatctgta gtaaatattt tacatgatca
    31561 gcattttctg taaagttgat atcactattc attaattaac aactaatatt tgatattata
    31621 ataatgtgaa cactatgcaa aattgcaata tatcataaaa aattgcaaga aaaaaattca
    31681 gtccagttgg tattttatac accatagtgt agtgtcatcc tgaacgctga ttcaagattt
    31741 aacgttatat atagggattc cgggtcaagc caggaataac cggctggtgt atatttctat
    31801 ttttggttga gttgtagtat tcagttcatt gatgaaacta tacgtttgaa atcttcgatt
    31861 atgaaataga tgagtatgct tgattatatg gaaatattca aaacctttaa taataacggt
    31921 atacactata tcattgtggg tggtattgcg gtaaacctgt atgggattcc acgtatgacc
    31981 tatgatatcg atatcttact gtatttagaa gaaactaata caaagcgttt tgttgagcag
    32041 atgatacaat gggattttaa accaaaaatt cctgttaaac caatggacat tgtagatgtg
    32101 aataagagga atgatcggat aaaaaataaa agtatgaaag cattttgctt tgctaatcct
    32161 gattgggcaa tttccaagat tgatattatt attgattctc ccattaatta tgaagaggca
    32221 ataaccagag caaagtttgt cacaataaat ggcataaagt tgcccattat atctattgat
    32281 gatcttatta caatgaagaa ggtgtcaatg cgagataagg atatagtgat atagaatatt
    32341 taaaaaggat aaaagatgaa cgataataca aataaaggat ttcgttatta cattgatgat
    32401 gctactttaa atgagtatca gcaaaaacat tatcatctcc ggcttcaatg gttgtatatg
    32461 ggaaataaat tgcgcaaggg atatagtagc accataaaag ctattcagga tagtttcagg
    32521 aggggtgaag gtagttttaa acagtaacta tcgccagaac ctaaagaata aaacgtgcaa
    32581 tttgataaac aataaaactt actatccatg caactgtaat ctgatatgca actgaaaaaa
    32641 aagcccagcg ggtaccaatc tcgcgagcaa tagcaatcac tgtggctaaa cagggaatat
    32701 atatcatgac aaatatcatg aatgctaatg ctgcgtgagg agtgatacca taacggggat
    32761 tgccaagctt ttgtgataag ctgacagtat tttcatcatt ggcagcatga tacaatacac
    32821 ccattgtgct tactacaatt tcttttgcta caaaacctgt ggtaagcgat atccccatct
    32881 gccagttaaa tcctaacggt tgtaacagag gatagataaa agaaccaatc ctgccgataa
    32941 aggtatactg taccatttcc tggcgctttt gtatctctaa atcttcaatt attgtgttat
    33001 actgtttatg aattgtctgg attgtgtttg ggtcagcatt ttgtttttga taagtactca
    33061 atgtttgatt caactcaatt attttttgct gtatcattgt gtcaaattgt ttttggatat
    33121 ggtaaggttt tggatattcg ctcaataccc atataataat tgaaaaagct aaaattattc
    33181 cacccatttt tcgtaaatag cttgatgctc tgtcccacat gtgtataaca attgttttag
    33241 tggttggcat gcgatatggc ggaagttcca taacaaacgg tgttgatttg cctttcagaa
    33301 tagtgttgtt gaatagcttt gccgaagtaa atgccagaat aatgcccaat gcataaataa
    33361 acaggatgat aaaacctgca tgattaggaa aaaaagtacc ggcaaacaaa atatatacag
    33421 gtaatcgcgc agagcagctc ataaatggat tgataagcac cgttagtatg cgatcgcgct
    33481 tattttccag tgtgcgggtt gccattattg ctgggacatt acatccaaaa cccataacca
    33541 gcggaataaa cgatttgcca tgcaggccca tgctgtgcat tattttgtcc attaaaaatg
    33601 cggagcgtgc catatacccg gtatcttcca tgaagctaat tcctaaaaaa aggatcagaa
    33661 tattaggcaa aaaaacaata acacttccta caccaccaac aattccttga acaatacaat
    33721 tttgtaattc gccaggaggg ataatttcca tcaaataatg acttaaccat tctacaccca
    33781 cttcaataag tttcatgggg tattcgccca gtataaatgt aagattgaaa aggataaaca
    33841 ggatgacaaa aagtaatgga aatcctaaaa atcgattaag aacaaccgag tcaatttttt
    33901 ttgtcaggtc atgacgtttg attggctttt tttgtactgc ctcttttaat gcaccggtga
    33961 tgaagccgta acgcatgtcg gcaaaaactg tctggatatc atcatgaaaa gctttcgaaa
    34021 gattgttgcg gatggtataa gccttccgta ttatttcatt gccgtctggc aacaattgca
    34081 ctcttttttc agcatcgatg tcgccctcta tgaggtttat tgccagccat cgtgaaggat
    34141 attctaatct gtttttatca ataagctgtt gtaattgttg aatagcctgt tcaatatcat
    34201 tgccataggg gatagttatt gtatgatttt ttgtttgttt ttcaaaattt tctacaatgc
    34261 tatctttaag ttgtgggata ccagttcctt ttgttccaac cgttggtata acaggcatat
    34321 tgaacaattg acttaatttt tgtatattta tgatgagtcc tttatcaaaa gcttcatcaa
    34381 tcatattgag tacaatgata accggcgctt caatatctat cagttgcgtt gttaaataaa
    34441 ggttgcgttc tacattgcct gcgtcaataa cattgataat gaagtcaggc ttatcattct
    34501 ggataaaatt gcgggtaata agctcttcca tgctgaacga tgtaagacta taggcaccgg
    34561 gtaaatcaac aaccttgatg gtataccctt tatggttaaa aaaaccttca cggcgttcta
    34621 cggtaacacc agcccagtta gctacttttt gacgtgcacc ggttaaggca ttaaaaatgg
    34681 ttgtttttcc gcaatttgga ttgcctatca gtcctactgt tatcgttttt tttgtcatgg
    34741 tgggctactc gtcaattggt ttaacaatga tataattagc ttcctcaaca cggatagaaa
    34801 ttaagaaatc ctttatttca atttctatag gatcatggag cggtgcatat tttattatat
    34861 gtataatttc accctttcta aatcccatct caagcaatcg tttctttaat ggaccgcttc
    34921 ccagtattgc aactatttca gccttttgcc cttcttttag ctggcttaat ctcctttcct
    34981 ctaatgtgat gtccagtctt tttatccatt gttcattagc ctgccgttca tgtttataaa
    35041 attcaacaaa ctgttgcatc cgtgtactgg catcgttact taaatggtgc tccagcttgc
    35101 atgcctgctc ctcggcactt tttttatcca tgttcaggat tgattgaaaa aattcgctta
    35161 aaaatgagtg cttatggtaa actgaatcgg caatctcttt accacggggg gtaagctgta
    35221 catggccata tttttcgtaa tcaaccagtc ctttttcctt tagtttttgc aatgcagcag
    35281 taacacttgg catggtgatg ttgagctttt gagcaatatc ttttacacga acaacatcat
    35341 tttcgtttaa aagcagagca atgttttcca tatagtcttc catatttgca gtaagaccgg
    35401 tatcattcaa ctcattggtt ggattgttta cccatgggct ggattttctt tttctcatct
    35461 tcatattatg tgcgatagtt attattaatc aaaaatatag tgttaattta atgaatataa
    35521 tataattagt caagtctaat tttattattg gctgatatat ggttattgta ttaataggtt
    35581 tttttaactg atgactaatg aacaaagttc t
//
LOCUS       DAOSOX010000006        34197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1300_length_34197_cov_9.624802, whole genome shotgun sequence.
ACCESSION   DAOSOX010000006 DAOSOX010000000
VERSION     DAOSOX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 34197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1300_length_34197_cov_9.624802"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..448
                     /locus_tag="PLX22_00955"
     CDS             302..448
                     /locus_tag="PLX22_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18501.1"
                     /translation="MEIMRISGADVVPAGPAVNNQGQQENTVEKEKPEQPEKVNKSTI
                     DTYA"
     gene            460..1269
                     /locus_tag="PLX22_00960"
     CDS             460..1269
                     /locus_tag="PLX22_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18502.1"
                     /translation="MPVTKAEKVAYNDFVKGYRAKLEELTKQIKEAEQKKKKMPGIKG
                     YINLELILLYIKYIYTLLNMNTVSVNMLKVKNERFLNDARKEFYRVLQLFEEIVGKDI
                     DRMLKENEEYLAAINKLNPQQTLALVKEIHVLLQKIMDALGESSKWKWSFVELQGRVA
                     VITKNMINFSELQKYRDPRKAFYYERQDMLKLCKQSLSEAARQYRNKYELSTKSSEDI
                     LKSIELLTALRRIHILFGESEEAEKLKNTIEALRLRLEADEKEEEEKKKKK"
     gene            1520..2563
                     /gene="hrcA"
                     /locus_tag="PLX22_00965"
     CDS             1520..2563
                     /gene="hrcA"
                     /locus_tag="PLX22_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT18503.1"
                     /translation="MHTKQLNDREAQVLKAIVYEYIATGKPVGSRSFVQKYSFSLSPA
                     TMRNIMFDLEQMGYLSHPHTSAGRIPTDKGYRFYVDSLLDTYETVAKTDIEVREEMLA
                     REIELDKIFMSIVKMLSLISKYTGIALMPKPDFTVVKHIELISLTSNDVLFIIVTRTG
                     IVIHKKVKLSSSINQDDLYKYSRFLTAELSGYSLVEIRQSLINQLRTVTDVETSVRDV
                     ALDITELALKDEQEPDIYIDGIENLLHIPDIVEAEKLHEIVRFIEQKDTLRKIMEDLR
                     QKEGVFTLIGDEIKDIAIPCCSIIASSYKIGNSPVGVVGVIGPTRMDYEKVVPLVDYT
                     GKVVSNFLTQMTK"
     gene            2609..3367
                     /gene="grpE"
                     /locus_tag="PLX22_00970"
     CDS             2609..3367
                     /gene="grpE"
                     /locus_tag="PLX22_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT18504.1"
                     /translation="MSNKHDKRHKREDDNIHDAINQEKIQTMNEEKIKVQENGDSSLN
                     QSAGNDQVESSSELIQQKEDQLRKLQEELNKKNEELDALKDLLVRRQADFENYKKRVI
                     RLQEEDRKLAIKDIARDIININDDLLRAIDAACNIQDAKDLADAHNLFVEGIKMISRQ
                     IEDMLKRYGVEEINSLGQAFDPEYNEAVEIDESPDVVKDTITKVYQKGFKLDKYVVRA
                     ARVKVTRPAKQKVEDDNANNANNATEENTAASQN"
     gene            3437..5374
                     /gene="dnaK"
                     /locus_tag="PLX22_00975"
     CDS             3437..5374
                     /gene="dnaK"
                     /locus_tag="PLX22_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT18505.1"
                     /translation="MTMSKIIGIDLGTTNSCVAVMVGGEPVVIPNSEGQRTTPSVVAF
                     TDKGERLVGQVAKNQMITNPENTIRSIKRFMGRSYGEVQNEIKMVPFTVADDGKGGVK
                     IKTIAGEFTPQEISARILQKMKQSAEDYLGEKVTRAVITVPAYFNDSQRQATKDAGRI
                     AGLTVERIINEPTAAALAYGLDKKERNHKIAVYDLGGGTFDISILELGEGVFEVKSTN
                     GNTHLGGDDFDQRIMEWLISEFKKQTGIDLHTDKMALQRLKESAEKAKIELSNKLQTE
                     INIPFITADQNGPKHLIMTLTRSKFEGLVEDLIESTKEPCIKALEDAGLTPGDIDEVI
                     LVGGSIRIPAVQELVRRVFGKEPHKGVNPDEVVAVGAAIQGGVLAGEVHDVLLLDVTP
                     LSLGIETLGGVMTKLIERNTTIPTRKSQIFSTAADNQTAVSIHVLQGERELASQNRTI
                     GRFDLIGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGKEQKIRIEASSGLNEEEI
                     KKMVKDAEQHAEEDRKARQLVEARNEADALIYSLEKLIKENEGKIDAAEKSSIESEIE
                     NLRKAMESSDINVIRDAKAKLERASHSFAEKLYKQGAEQYAQSQSQQQSDTSGTTEES
                     TQGGEKVYDADYEVVDEDKKK"
     gene            5456..6574
                     /gene="dnaJ"
                     /locus_tag="PLX22_00980"
     CDS             5456..6574
                     /gene="dnaJ"
                     /locus_tag="PLX22_00980"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT18506.1"
                     /translation="MATTKRDYYEVLGVPKNATEDEIKQAYRKLALKFHPDRNKGSKE
                     AEEKFKEATEAYEVLRDPKRRAQYDKFGHEGVAGFEGFGRGAYTDFSDIFGDFDIGDI
                     FESFFGAGSGSGTRTRSSRTRRGRDIQYDLSITLDDAANGKEVQIEIPRHEVCSSCGG
                     TGSASGSKPTVCPVCNGTGQIRQTQGFFSVTQTCYKCRGEGKIITSPCRACRGTGLEE
                     KHRKITVKIPAGVESGSRLKISGEGEQGPGGGSAGDLYVVVHIQKHPLFERHGNDLIC
                     VVDIPFTIACLGGEIEVPTIYNKKAKMKIPAGTENGQIFRLKGNGMPYLGSYGKGDQL
                     VKINIKVPKKLTPRQKELLKEFARIEGETVDTDREFYS"
     gene            complement(6586..7632)
                     /locus_tag="PLX22_00985"
     CDS             complement(6586..7632)
                     /locus_tag="PLX22_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18507.1"
                     /translation="MEIITVFGVRWRVAPLWEVLVTLFVILVIIGFFALLRYNQYLKL
                     KKSSIEQLFIYKARQRGLTGYQIRILQHIISLSKLSTPEIIFNNVTIFETGLVKFLQY
                     IKKSRDTFNEDLKDIFKDIVITYERIYNPVEYQEQLNSPDELTTNTMVCGYSATGRAF
                     IAKITQITGDAIHCKYFRKPEDIPADIIAEQVTIFFFRNGDAEYSFDSKIIAIQENTI
                     VLQKPQAFKRGKEVHHPYIQTLIPCTVQLKNDESKNSEEVIIQHDETVSINGTIERLN
                     EYEAVIRVEKRLEYQKEYILFFTLENFKIEVLSTIIASRTITREKIIYYTFKFVQMSE
                     AAKNVISKYVKERL"
     gene            complement(7651..7834)
                     /gene="ssrS"
                     /locus_tag="PLX22_00990"
     ncRNA           complement(7651..7834)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLX22_00990"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7991..10864
                     /locus_tag="PLX22_00995"
     CDS             7991..10864
                     /locus_tag="PLX22_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18508.1"
                     /translation="MKKFFKHKICYSAILCVIITVPLFVYPFGKNKVTVEVFTWHILK
                     TIHFNVYYPYGMEKLAATTATIAERAYGYLADTLQHQLNEPVPIIVFPSHIDFQENNI
                     LMQIIGEGTGGFTEAFKNRVVIPFSGSYDEYQHVLVHELVHAFQFNILMHDSSGKLQS
                     MMSMGAVPLWFTEGMSEYLSIGFDETADMTMRDFFFNDQYVSLMDFTTYRTGNLYYYY
                     KVGQAFFYFFETVYGKGKIGEFLRDIRDMGNFEKALKVHTKKDLEAIDKEFRHFYKKR
                     YYPLMVGRNFDEEEGERVTDHKKTRSVFNTCPAISPDGKKIAYIDNRDIYASISVITI
                     GEKEAEEKARVLLKGNETSQFEGMHLLDNYLSWTPDGKGIVFVSQSNNRDVIFILDSR
                     NGTIIKKIVLPFRSIKDPALSPDGNYISFIGQTNTQSDVYIYRLNDTMLQQITNDVYT
                     ERYPRLDKKNAFIYYSSNYPTNDYTGTDYAIIRHNVLTGERQVIVKAQSKNLQVDVAG
                     DGNTIVYISNRDGIYNVYRYTIATGEEIKITTGTTGIFYPRLFPEGNAFAFVSYQNGG
                     YDIFIKNEIKPVTETPLFNSEHIPVMLPPNYFPLSKAVWDSYETRISSDWVFVGLGGT
                     VGYGFTGFAQMSFSDYLGDNRIIVTTDFLRDDESNNFMNYDVQYLYLRYRWDWTFGCF
                     RQKSPYGIFTLANLNDIIHNAYWSTLSMDHYGAYVIASYPFTKFSRFSTRVSSSRYEQ
                     DYGYSDERPDVYGNLNSISVSYNYDNVLWGFLAPLRGTRGQIMLTQVFNLTGQDYSFT
                     SIDIDLRHYFFIDKQYVIALRGVGGKIMGSEKEYFKYYIGGFNTLRGHPFVEYGGSNV
                     FLFNAEFRFTFIESINMGWPLFLKIGNIGGVLFIDTGSAWGNSYTFFNKETDRFEDFK
                     MDMGFGFRLTVYPIVILKLDYAWPYYYGKFGEKEILFSLGYEF"
     gene            10901..11485
                     /gene="hisB"
                     /locus_tag="PLX22_01000"
     CDS             10901..11485
                     /gene="hisB"
                     /locus_tag="PLX22_01000"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT18509.1"
                     /translation="MERSAHIERKTNETDITLKCILDSGDASIITTGIGFFNHMLTLF
                     VTHGRMNIQLNARGDLEVDDHHTVEDIGICMGKAFNKALGDKSGISRFGQAVIPMDEA
                     CTAAVVDISGRGCFVYKGDKLTGAIGQYQEELTLEFFQSFAHNALITLHIQQMYGDNR
                     HHIHESIFKSAGLALYRAYSLSGRTGIPSTKGVL"
     gene            11482..12090
                     /gene="hisH"
                     /locus_tag="PLX22_01005"
     CDS             11482..12090
                     /gene="hisH"
                     /locus_tag="PLX22_01005"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT18510.1"
                     /translation="MITVIDYGMGNLRSVVKAVEKYTSDVQVSSDPESIASSNALIMP
                     GDGAFGQAMENLTQSGWIEPLVRFIGNGGYFFGICLGYQLLFTSSEEFGNHKGLDIIP
                     GTVIRFPDDLKVPHMGWNQVELIKEHPVVNGIPQNSYFYFIHTYYPVIGDSSWIMGQT
                     AYGVTFPSIVGRGNCIATQFHPEKSHRYGLTMIENFVRMVCS"
     gene            12081..12800
                     /gene="hisA"
                     /locus_tag="PLX22_01010"
     CDS             12081..12800
                     /gene="hisA"
                     /locus_tag="PLX22_01010"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT18511.1"
                     /translation="MLVIPAIDLKEGRCVRLVQGDPLQEKVYSYDPVTIARQFEDAGA
                     QLIHVVDLDGAFEGQPVNMHIVKEIAKSVSIPIEIGGGIRTEAAIKMYLDEGIERIIM
                     GTILIEESFREMIQRFKEYIIAGVDARDLHVATHGWKKIIDINAIEFIKDIQNIGIAE
                     IIYTDIATDGMLQGPNFRSIERILADVPGVSLIASGGISTMQDIQKLLHYSGSGLKGC
                     IVGKAIYDGMVDLRSAIKLCS"
     gene            12812..13885
                     /gene="dgcA"
                     /locus_tag="PLX22_01015"
     CDS             12812..13885
                     /gene="dgcA"
                     /locus_tag="PLX22_01015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HOT18512.1"
                     /translation="MNDDVSLYGEEIKFPAEIEQYERKIYDLKQLIEISKGLNSTLDY
                     NILIDSILLTCMGQMQLLKAGIFTHRSIDRQEFVLHRNYKGFEIDHKIEYLIPANSKL
                     IQFLDMNFKCYTLDELKDILRDEPSLESLICLDPSLIVPLKGKGTLNGIIILADRINQ
                     KGFSESDKEYMLNIASLAGIAIQNAYLYELATTDMMTRLKIHHYFQTALMEEREKSIK
                     SSLPLSLIMIDIDNFKDFNDTYGHTCGDLVLKNVAMVISGSVRQSDIASRYGGEEFAV
                     ILPHTEINEAFKVAERVRTSVQKSYILYKRKKLGVTVSVGVTQFNPSLDSTSKNIIER
                     ADKALYMSKQQGRNKTTVLLTDK"
     assembly_gap    13963..14062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14063..14574
                     /locus_tag="PLX22_01020"
     CDS             <14063..14574
                     /locus_tag="PLX22_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18513.1"
                     /translation="YIKFISGMLILTLSINFIIYPVMAQTIEEDAGKKYKGTPYQDEF
                     DKKAKERREEPAMMERDPRLACLLSLIIPGGGHIYLRNDLKAVGIFLLTVTAYSFSAY
                     YLYVALNKTDSSTEKKSKLILSGLLFVVGAIIHVVGIVEAYNDAIEINEQQYYFGAER
                     SPCPYIAKK"
     gene            15081..15154
                     /locus_tag="PLX22_01025"
     tRNA            15081..15154
                     /locus_tag="PLX22_01025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15115..15117,aa:Ile,seq:cat)
     gene            15248..15562
                     /gene="rplU"
                     /locus_tag="PLX22_01030"
     CDS             15248..15562
                     /gene="rplU"
                     /locus_tag="PLX22_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT18514.1"
                     /translation="MYAIVEIAGKQYKVEKDKTVAVDKLDAKDNNEIAIDKVIMFVDG
                     EKVLIGQPYLSNVVVKAKVDGMVRGKKVRGIKFKRRKNYTRTFGHKQHYTSITIVDMS
                     VQ"
     gene            15562..15915
                     /locus_tag="PLX22_01035"
     CDS             15562..15915
                     /locus_tag="PLX22_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOT18515.1"
                     /translation="MINVTLQGIILRDGNIALSGSEIVITITGHSTKAAKGNDIVCAG
                     VSALAQSVVVALEYWAIVHSIVQDDGLLRFSIHVPLLNDREITRCESIVSVLVIGLNE
                     IRKQYSQFLTINVNE"
     gene            15961..16221
                     /gene="rpmA"
                     /locus_tag="PLX22_01040"
     CDS             15961..16221
                     /gene="rpmA"
                     /locus_tag="PLX22_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT18516.1"
                     /translation="MAHKKGGGSSRNGRDSHAKRLGIKVSGGQFVKSGSIIIRQRGTK
                     LHPGLNVKRGSDDTLFAAADGVIKFERLGKERKKVSVYPVQS"
     gene            16622..17605
                     /gene="obgE"
                     /locus_tag="PLX22_01045"
     CDS             16622..17605
                     /gene="obgE"
                     /locus_tag="PLX22_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT18517.1"
                     /translation="MAKFTDTITIKIKAGNGGAGAVSFRREKYIPKGGPDGGDGGKGG
                     DVIIQAQQGYYNLGHLFKDRIYKAKNGGYGMGQNRHGKDGQDLIIKVPAGTEVFDTQT
                     GEPLADLVNEGDSVCVAKGGIGGKGNAFFKTSTHQTPRFAQPGMQGDERTVTLNLKLI
                     ADVGLVGLPNVGKSTLLAKITNAQPKIANYPFTTLIPNLGVIEQGEKVITIADIPGII
                     EGAHKGLGLGLSFLQHIERVKVILFCVEAVDADPLYTLSLLKAELSTYNDNLSKRHSI
                     LLLTKSDMVSESILQTRIQLLQKEKYDCVPISSLTGYNIDILIEKLFLIME"
     gene            17610..18716
                     /gene="proB"
                     /locus_tag="PLX22_01050"
     CDS             17610..18716
                     /gene="proB"
                     /locus_tag="PLX22_01050"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT18518.1"
                     /translation="MPRSQYLKRVKTIVVKVGSSLITNSDGISAECVDKLVDDVVFLI
                     KKKYKVVIVSSGAISAGSSVMKKKRMLCTIPEKQALAAIGQSILMDVYRGCFNKRGYQ
                     VGQILLTEDDVKHRRRFLNARHTLNALLDMGVVPIVNENDSVVIKEIKFGDNDTLSAH
                     VANIVQADLLILLSDVDGFYWDEHDEEPVSEIFEINDEVKKRCRGAGSEHGVGGMVTK
                     IKAGEIVIKSGVMMIIANGKTENILKQIMQGKKIGTLFSGKTQLKSKKRWIAFNVKPK
                     GKLTIDDGAVKALKENKKSLLAIGIMNVEGDFDLGDAVEIVDTHGMVIGKGIVNYTSD
                     ELHKIKGKNTQEIKKIFGSKYYEEVINRDDLIVF"
     gene            18900..>20242
                     /locus_tag="PLX22_01055"
     CDS             18900..>20242
                     /locus_tag="PLX22_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002090384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT18519.1"
                     /translation="MINTILSLFFGTKHERDIKKLKPIVQQINALEDEVKKLTHDQML
                     AKTAEFRGRIENGQPVDDLLPYAFAMVREAAWRTIGMRHFDVQLMGAIVLHQGRISEM
                     KTGEGKTLVATMPVYLNALAGKGVHVVTVNDYLARRDSEWMGPIYKYLGLTVGVIQHN
                     MDYYQRQQSYRCDVTYGTNNEFGFDYLRDNMVEHRSLRVQRELYYAIVDEVDSILIDE
                     ARTPLIISGSTDESTKKYYLINKIIPGLREGVDYEVNEKDRNCLLTEEGVRHVERLLK
                     IDNLYDSRNIELVHHVNQALKAHTLFKRDIDYIVKDGQVVIVDEFTGRLMPGRRYSDG
                     LHQALEAKENVTIARESQTFAQITFQNYFRMYKKLAGMTGTADTEAIEFKKIYNLDVV
                     VIPTNEPVRRTDYPDRIYRTEKEKFNAIVKEIQQLREDGRPVLVGTISIEKSEKLS"
     assembly_gap    20243..20342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20343..21281
                     /gene="secA"
                     /locus_tag="PLX22_01060"
     CDS             <20343..21281
                     /gene="secA"
                     /locus_tag="PLX22_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002090384.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT18520.1"
                     /translation="FYLSLEDDLMRIFGSERIAGVMQRLGMEEGQEIESRMVSRAIEN
                     AQKRVESRNFEIRKHLLEYDDVMNSQREYVYKERNAILDGEDVSIKIKQYIGDVVESW
                     VPYFTQGKRNAEDWDMEGLNGWLYSKFGFDLQSIAKDGARLSHDEIIDGITNKLMQLY
                     SAKEEEIGSEHMRTLERLVSLQVIDAKWRDHLLVMDDLRDGIWTAGYSERNPLVEYKL
                     RGFELFNEMLDNLKLDIIEMLLKVRLQKVEEIAIQPEKIGIPLGQEYHPEVEQFGAGG
                     IPVATPPVTIASHGDDKTREVTGGVRRKKTRRSRRG"
     gene            21284..22648
                     /gene="glmM"
                     /locus_tag="PLX22_01065"
     CDS             21284..22648
                     /gene="glmM"
                     /locus_tag="PLX22_01065"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT18521.1"
                     /translation="MSLMKSISGIRGIVGQSLTPDLITKIAMAFSRHIGGGTVVVGRD
                     SRGSGGAIAGCFDAVLSLCGCNVIDIGIVPTPTVQIMVEHMKAKGGVVISASHNPIQW
                     NAFKLINKSGSFFGPDEMNAFLQLVDEVSPFGVAWNTIKPIQCDDTAMDIHIQKVLTA
                     IDVDAIRKKHFKVVLDSVNGAGSIITPELLKHLGCRVIPIHCDMQSGVFPRGAEPLAK
                     NLKQLANTVKQHKADIGFAQDPDADRLAIVNEHGKPIGEELTVTLAVMRCLLKKKGSV
                     VVNMSTTRAVEDVAHSFGATVYRSKVGEIHVVEAMKKHRAVIGGEGNGGVIAPEVHYG
                     RDSLVGIGYCLELMALSNRSISEIVSSLPVYYMIKDTVAIEQGIDTGAIAHEIASQYH
                     DEMINTLDGIRIDFIHHNRFKGGWVHLRASNTEPIFRIIAEGTTKEQCTAMIDAFKTM
                     KIAP"
     gene            22784..24556
                     /gene="aspS"
                     /locus_tag="PLX22_01070"
     CDS             22784..24556
                     /gene="aspS"
                     /locus_tag="PLX22_01070"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT18522.1"
                     /translation="MFNDRSYCGELTLDDAGKQVTLAGWVDTKRDLGGIIFIEMRDVT
                     GIIQVVFDVSKNGEAYQIADRAKSEYCIYTCGVVSKRTEDTINPDLKTGMIEVVAHEV
                     VILSESEVPPFPIDVYTNLNEDVRLKYRFLDLRRAEMQQAIVIRHKVMQATRNYLSSN
                     RFFEIETPILNKSTPEGARDFLVPSRINRGMFYALPQSPQLFKQILMIAGFDRYFQIA
                     RCFRDEDLRNDRQPEFTQIDCELSFITPQIIMQVIEGLIKDMVKVLGYDIHTPFPVMT
                     YDEAMQRYGKDAPDTRFGLELVDVSDIFAGSSFNVFKASLDNGGIIKAIVVNDEGKIS
                     RAMIDEYINYVRTFKAHGLPSVRYTGSNFETGIAKFLSDSEKQKLIDRLSLDSNSLII
                     FSCDKQKVVNDALGNLRLKIAQQLTMIDDNKLNFLWVVDFPLLEYNAEEGRFYAMHHP
                     FTAPKPECEAMLDSLTPDNVDMVKAQAYDVVLNGVEIGGGSIRINRPMVQKKMFGILG
                     ISEEDAKIKFSFLLDALKYGAPPHGGIALGLDRILMILLKRNSIRDVIAFPKTQKGQC
                     LMSEAPSPVSPEQLKELSIKVVEV"
     gene            24556..25515
                     /locus_tag="PLX22_01075"
     CDS             24556..25515
                     /locus_tag="PLX22_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOT18523.1"
                     /translation="MENTKFEIDDISIYKRICGYKDKNIKTIEKLLDVKIIPRGNTLI
                     VEALNDNAVKALQLLHCMGDYLYMRDSLYEFDDFDIRYLALSINQGIKIDADEINRLK
                     IFIPETGKTILPKTINQAHYMSAIHKSPITISIGPAGTGKTYLAVAVGVKFLLTGRVE
                     RIILTRPAVEAGESLGYLPGDFIQKINPYLRPLYDALFDILPYERISKYMEKGIIEIA
                     PLAYMRGRTLNKAFVILDEAQNTTSSQMKMFLTRLGNNSKMVISGDITQIDLEKPKQS
                     GLLAAQKILHGIDEITFIEFLKEDICRHPIVEKIIAAYEKHTE"
     gene            25534..27780
                     /locus_tag="PLX22_01080"
     CDS             25534..27780
                     /locus_tag="PLX22_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT18524.1"
                     /translation="MLNSFMAFKRGIEFALKKKSIRLSFIYTIVVIVSATALLTINRT
                     GATYNYEAGDIAREDIRAPMNIEYPVALETEIEKNRIAESMPAVFDLNESIVEDKLKI
                     CNILCEAVIKVKQQMPDTNPFKQVLLLRTMLPDYLQYSDKVLIGLLQYDEQLLHRDMN
                     KVILQVYNNGILEKQYTNPLKLKSNFIVIRVIGNASELKERQVSIDALYDINKIKTLV
                     PVICKEVCNQPSNEVVYAISSVVMSLLQPNLFYNEEETKKRITDAIQSVKPVMRVIKK
                     GQSIAREGDTITEDVLKKIDIINKNSRFSGFSFVLGLLLTQLGFLIIFGYFLITYYTN
                     LVVDNKMPLIVFTLVMAFIIITFFMARWQGRMFEGLFFPLLLPIPLITMILAIMYNIH
                     IAMLIGMYIVFFVAIITKGDFTSLLLAFSSALSGVFVVGDVERRSDFLRGGFTLGLIN
                     SVIVVSVGLMNELSFAMILNNIGLAFVSGIINAIVVFGVFPIYENLFGLTTKFKLLEL
                     SDLNAPIFREMLIKAPGTYNHSIMVATLAESACKEIGANALLARVGGYYHDIGKIQDA
                     NFYIENKVTDPRAKKLSALEYCKLIISHVEKGVELARKNNLPEMVIDFIREHHGQTVM
                     TYFYHQALEESGASGDIIQKSDFQYPGPKPHTRETAVVMLADAIEAAARSIQEPTIEK
                     LESLIKRIINNRLNEGDLDHADITMNELNKIQHSILRVLQGIFHSRIEYPTDDEINRL
                     EQRSEHGH"
     gene            27770..28216
                     /gene="ybeY"
                     /locus_tag="PLX22_01085"
     CDS             27770..28216
                     /gene="ybeY"
                     /locus_tag="PLX22_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT18525.1"
                     /translation="MAINITIESEDISVSKYLKTFCKKNIKAILRYLHIDNVAVTLVL
                     TGDASIARFNRNYRKKKGPTDVLSFAYREAPTPLADGLPEYLGDVIISLETAQKQAQE
                     YNVTLHDEMLRLIVHGMLHLLGYDHEQYYKKKQMQKKEEELLQHLR"
     gene            complement(28235..29956)
                     /locus_tag="PLX22_01090"
     CDS             complement(28235..29956)
                     /locus_tag="PLX22_01090"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008730891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT18526.1"
                     /translation="MQQWQQPPFDSETIHEINQLVQQNNEKELYERFYTNLEFGTGGL
                     RGIIGAGTNRMNIYTVGMATQGLANYILQKGSAEQGVVIAYDSRRMSDVFSKEAASIL
                     AANGIMVYLFSDITPTPICSYAIRYFKATAGIVITASHNPPEYNGYKVYWNDGGQVVP
                     PQDKEIIDEVQKINAITQIKKMDFHKAAGNKLIQLAGNEIINSYADKLSGVIFKSQEL
                     SPINIVFTPLHGTGYNIVPMILQKFGFTNVNVVEKQAIPDGNFPTVASPNPEEPEALT
                     MAIALAKTMNADIVLATDPDADRMGVAFKDTNGEYTLINGNQIGSMLLYYILLQRQHH
                     NRLPRNSFVVKTIVTTELQQAIADYFHVSMENVLTGFKWIAKKIRDYEHTHTFIFGGE
                     ESYGYLPLDFVRDKDAVSSCYFFAEMTHWLHKQNKTLNDFLYEIYSRFGYYNEKLVSL
                     TLKGVEGIEKIKTIMKHFRSNPPQQFAGIKVVSILDGKAGIVDIASGATKPANLPPSD
                     VLQYSLENRSKVTMRPSGTEPKIKFYFSSCKQADDIKQASQEVNHCNDQLADELLNTI
                     KTIVNID"
     assembly_gap    29961..30060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30061..31386
                     /gene="tilS"
                     /locus_tag="PLX22_01095"
     CDS             <30061..31386
                     /gene="tilS"
                     /locus_tag="PLX22_01095"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT18527.1"
                     /translation="SVSAGKDSMALLHILFVLRVRLDCTIAIYHLDHTVRGDESFDDL
                     LFVIQQAARYGIGAFVERYNFNQHKEKGKSFEQQAREYRYARLQMVAHEYGYTKIATA
                     HTCDDQIETLVMRIFQGTGLQGLQAIPLINNTIIRPLLSLHADDVYAYCTCNELSYRH
                     DLTNDDTHYLRNFIRHEVIPLVKKRFPTFDKALLNLQQQALGAVKSIEQLMAKVYPDC
                     IKQGGNHVEVPEDALSLNEYLFKFYCASIISNYLHRYCDTAMLDALYRAHVSDKKNVT
                     LFEGNGIFVYRRVKQSGAVLLFCSDPIMGDSNYEYTIPLGEDIYIDELGLTVRCMVME
                     STDAMPCTESKTIVLRYTGYGDIVVRNRRSGDTIFRKAGRRTLKRLYIDYKLTPEQKQ
                     KLPIIVVDNCVAAVLFDTIISQKPAISQTFLPLPHEKMLVIHYWYNHND"
     gene            31597..33516
                     /gene="ftsH"
                     /locus_tag="PLX22_01100"
     CDS             31597..33516
                     /gene="ftsH"
                     /locus_tag="PLX22_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT18528.1"
                     /translation="MNKAAKQVALLLLIVVLAIAIFRMNEVNSQKVEQLQYSDFIKKV
                     YENKVKSVSIVNGKKIEGAYTKGDKVYLFETVIPYADPDLIKTLIKNKVEVKGEEVDD
                     NFFWKGLLNFLPWLLFFGFIWFFMIRQIQSTGNKAMSFGKVRAKLQPETKNKVTFADV
                     AGVDEAKVELQEIIEFLKDPKKFITIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFEQGKRNAPCIIFIDEIDAVGRLRGAGLGGGHDE
                     REQTLNQLLVEMDGFETNEGVIIVAATNRPDVLDPALLRPGRFDRQVVVDIPDIKGRE
                     KILAVHTRKIPLAKDVDLKVIARGTPGFTGADLANLVNEAALLAARRNKKRVTMLEME
                     DAKDKVLMGPERKSVLISAEEKKVIAYHEAGHAILGILTGTDPIHKVTVIPRGRALGL
                     TMHLPKEERHIHNKGYWLDQITILFGGHVAEKIKFGQVTTGASNDIERATDIARRMVC
                     EWGMSETLGPLAFGKKSEQIFLAREIAQHRDYSEQTAQLIDKEVHEIVTSCRDRAIIL
                     IEEHADKFELIATNLIERETLNAQEIELLMKGEPLPLIHNGDSEPPDEDEPKIGKAKR
                     KTVSKSLLDKEIIPT"
     gene            33534..>34197
                     /locus_tag="PLX22_01105"
     CDS             33534..>34197
                     /locus_tag="PLX22_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18529.1"
                     /translation="MIDDELDRILQDIEPEQDYLTKKVNASKKYEEYILDEITEDNKE
                     ITYTPEKKEISLIARYFVNGLVNGIHRHIELSRSIRSRMDIAEIPEQIVPILKKSSYA
                     IFKLIEDIEILSDKCINEINETNLKRRATKDFPLYFADDFPDDNRLDFIVLKDTYVLL
                     RLFNSQIKKDWVELEKQTSVFNFSEGGKQLAFQLNDSIVEALQLCKATDILIELIANH
                     LGL"
BASE COUNT        10791 a         5501 c         7513 g        10092 t
ORIGIN      
        1 gaaaaaatac ttgaagatca aaaaataaaa attgacagta aggtaagttg aagattatga
       61 agatttatac cgctaaagaa tttccactaa cagcggtgca atttcaatga aataatttta
      121 tagtgctaag gaaaataata acattaatat gagcgatagt aatttttaat ttttttcttg
      181 ctattttaca ggagctatcg catatattaa ttattacact atgatagtga acataccatg
      241 gtaacggtgc atagagtgta gaaaatgttt attattatat cttcagggag ggatggaaaa
      301 tatggaaatt atgcgaattt caggtgctga tgtagtgcca gcaggaccgg ctgtcaataa
      361 tcagggacag caggaaaata ctgtggaaaa ggagaaacca gagcagccag agaaggtaaa
      421 caaatcaacc atcgatactt atgcataagg gaaactatca tgccagttac gaaagccgaa
      481 aaggttgcct ataatgattt tgttaaaggc tatagagcaa agttagaaga gttaaccaaa
      541 cagataaaag aagctgagca aaaaaagaaa aagatgcccg gtatcaaggg gtatataaat
      601 cttgagttga ttttgctcta tatcaagtat atctatacgc tgctgaacat gaacacagta
      661 tcggtgaata tgctaaaagt aaaaaatgag cgttttttaa atgatgcgcg taaagaattc
      721 tacagggttt tacagctttt tgaggagata gttggtaagg atattgatcg tatgttaaaa
      781 gaaaatgaag aataccttgc cgctattaat aaactcaatc ctcaacaaac attagctctt
      841 gttaaagaaa ttcatgtatt gttgcaaaaa ataatggatg cattgggcga aagttctaaa
      901 tggaagtggt cgtttgttga gttacagggc agggttgccg taataacaaa aaacatgata
      961 aacttcagtg aattgcaaaa atatagagac ccgcggaaag cgttttacta tgaacgacag
     1021 gatatgttga agttatgcaa gcaaagctta tccgaagctg ccaggcaata tcgcaataaa
     1081 tatgagcttt ccacaaaatc atccgaagat atcctgaagt caatagaact tttaacagcg
     1141 ctgcgcagga ttcatatatt atttggtgaa tctgaagaag ctgaaaaatt aaaaaatact
     1201 attgaagcgt tacgcttgcg attggaagcg gacgagaaag aggaagaaga gaaaaaaaag
     1261 aaaaaataag ggtattctga aaacgttaat ttgttaaatc cctgtgtata caaaatattg
     1321 ctaacaacac ttcttttgat tgttcatagg atgattgtat aaataagcgg ttttatgtat
     1381 gtctatacac taaagatatg aaaatatcat ctttattcca ctatttttaa ggaaaatgac
     1441 ttgacgggcg ctattttttt tagtatatta ttagcactca tatagattga gtgctaatat
     1501 gtatatagag gttgctatca tgcatacaaa acagttaaat gatcgcgaag cacaggttct
     1561 taaggcaatt gtatatgagt atatagcaac aggaaagccg gtaggttctc gttcttttgt
     1621 tcaaaagtat tcattttcat tatcaccggc aaccatgcgc aatattatgt ttgacctgga
     1681 gcagatggga tatctttccc atccgcatac ctcagcaggc agaataccaa ctgataaggg
     1741 ttaccgcttt tatgtagatt cattgctgga tacctatgaa acagtggcaa aaactgatat
     1801 agaagtacgc gaagagatgt tggctcgtga gattgaactg gataaaatat ttatgtctat
     1861 cgttaagatg ctatcgctta tttcaaaata cacaggtatt gccctgatgc caaaacctga
     1921 ttttaccgta gtaaagcata ttgagttaat ctcgctaacg tctaatgatg tgttgttcat
     1981 catagtaacc cgtactggaa ttgttattca taaaaaggtt aaactgtcat catctataaa
     2041 tcaggatgat ttgtataaat attcacgttt tttgacggct gagctttcag gatactcgct
     2101 ggttgaaata cgacagagtc ttattaatca gttgcgtacc gtaaccgatg tcgaaacatc
     2161 agtccgtgat gtagcacttg atataaccga attagcgctg aaggatgaac aggagcctga
     2221 tatttacatt gatgggattg agaatctgct gcatataccc gatatcgttg aagctgaaaa
     2281 gttacatgaa atagtacgtt tcattgagca gaaagacacc ctgcgtaaga ttatggaaga
     2341 tctaaggcag aaggaaggtg tctttacgct tatcggagat gaaataaaag atattgcaat
     2401 cccatgttgt agcattattg cttcatcata taaaattggt aatagtccgg taggtgttgt
     2461 tggtgttatt ggtccaacgc gcatggatta tgaaaaagta gttccgcttg tagattatac
     2521 cggcaaagta gtttcaaatt ttttaacaca aatgacaaaa taatatagtt atgaattgtt
     2581 acattaccat tgtatggcag ggggcgttat gagtaacaaa catgataaaa ggcacaaaag
     2641 ggaagatgat aacattcatg atgcaataaa tcaagagaaa atacagacaa tgaatgaaga
     2701 aaaaataaaa gttcaggaga atggtgatag ttcattgaat caatctgctg gtaatgatca
     2761 ggttgaatcg agcagtgagc taatacagca aaaggaggac cagttgcgta agttacagga
     2821 agaacttaat aagaaaaatg aagagttaga tgctctgaaa gatctgctgg tacgcagaca
     2881 ggctgatttt gaaaattata agaaacgtgt catacgtttg caagaggaag atagaaaatt
     2941 ggctataaag gatatagcgc gggatatcat caacatcaat gatgatttac tccgtgctat
     3001 tgatgcagca tgtaatattc aggatgcaaa ggatttagct gatgcacata acttgtttgt
     3061 tgaaggaatc aaaatgatat cacgacagat agaggatatg ttaaagcgct atggagttga
     3121 agagattaat agtttaggac aagcgtttga tcctgagtat aatgaagcgg ttgaaattga
     3181 tgaaagtcct gatgttgtaa aggatactat taccaaagta tatcagaaag gctttaaact
     3241 ggataaatat gttgtacgtg ctgcacgggt gaaggtgacg cgcccggcaa agcagaaggt
     3301 agaagatgac aatgcaaaca atgcaaacaa tgcaactgag gaaaatactg ctgcgtcaca
     3361 aaattaacag ggatgaaaat gaacaatgat acatactatc attcaattat attcaatact
     3421 ttaaaagaaa aggagaatta ctatgagcaa aataataggg attgaccttg gaaccacaaa
     3481 ttcatgtgtt gcagttatgg ttggtggtga accagttgtt atacccaatt cagaagggca
     3541 aaggacaact ccatcggttg ttgcatttac ggacaaaggc gaacgtttag ttgggcaggt
     3601 tgctaaaaat caaatgataa cgaatcctga aaatacaata cgttccatta aacggtttat
     3661 ggggcggtcc tatggagaag tgcaaaacga aataaagatg gttcctttta ccgttgctga
     3721 tgacggtaaa ggtggtgtga agattaaaac aattgcaggg gaatttacac ctcaggaaat
     3781 ttcagcacga attttacaaa agatgaagca gagtgctgag gattatctgg gtgaaaaagt
     3841 aactcgtgca gtcattactg tacctgcata tttcaatgat tcacaaaggc aggcaacaaa
     3901 ggatgcaggc aggatagctg gtttaaccgt tgaacgaata ataaacgaac caactgctgc
     3961 agcattggcg tatgggcttg ataagaaaga gcgcaatcac aagattgcag tgtatgattt
     4021 aggtggtgga acatttgata tatcaatact tgaactgggc gagggggttt ttgaggtaaa
     4081 atctaccaat gggaacacgc atcttggtgg cgatgatttt gatcagcgta taatggagtg
     4141 gttaatatca gaatttaaaa aacaaactgg aattgatctg catacagata agatggcgtt
     4201 acaaagactg aaagaatctg cagaaaaagc taagatagag ctatccaata aattgcaaac
     4261 tgagattaat ataccattca ttacagctga tcagaatggt cctaaacatc ttattatgac
     4321 tctgacacgc tcaaaatttg aaggattggt agaagatctc attgaatcta caaaagaacc
     4381 gtgtataaaa gcactggaag acgcaggact tacccctggt gatatcgatg aagtaatctt
     4441 agtgggcgga tcaatacgaa tccctgctgt tcaggaattg gtacgacggg tgtttggtaa
     4501 agaacctcat aaaggtgtaa atccggatga ggttgttgct gtgggcgctg caattcaggg
     4561 tggagtttta gctggcgaag ttcatgatgt gttgctgctg gatgttacac cgctgtcact
     4621 tggtatagag acattgggtg gtgtaatgac caaactcatt gaacgtaaca caactatacc
     4681 aaccagaaag agtcagatat tttcaactgc tgctgacaat cagacggctg tgtcaatcca
     4741 tgtgttacag ggtgaacgcg aattagcttc gcagaacaga accataggtc gatttgacct
     4801 cattggtatt ccacctgcac cccgtggcgt gccgcaaatt gaggtaacat ttgacataga
     4861 tgctaacggg attttgcatg tatcggcaaa agatcttggt actggaaaag agcagaaaat
     4921 aaggattgaa gcctcaagtg gtttgaatga agaagaaatc aagaagatgg ttaaggatgc
     4981 cgaacaacat gctgaagagg atagaaaggc gcgtcaactt gtagaagctc gtaatgaggc
     5041 ggatgcttta atctattcac ttgaaaaact gataaaggaa aatgaaggga agatagatgc
     5101 tgcggaaaaa tcttcaatag aatctgaaat tgaaaatctc aggaaagcaa tggaatcaag
     5161 tgatattaat gtgatacgtg atgcgaaagc taaactggaa agagcatccc attcatttgc
     5221 agaaaagctt tataagcagg gtgctgaaca atatgctcaa tctcaatcac agcaacaatc
     5281 ggatacttct ggcacaactg aggaaagcac tcagggtggt gaaaaggtat atgatgcaga
     5341 ttatgaagtg gtagatgaag ataagaaaaa ataaactata atagtaaata attgtgcgac
     5401 aatggttgcc attggcgcac aattattgta atctttttaa ataggggtga tgaccttggc
     5461 tacgacgaag cgagattatt atgaagttct gggtgttcct aaaaatgcaa cagaagatga
     5521 gataaagcaa gcttatagaa agcttgcttt aaaatttcac cctgatcgca ataaaggaag
     5581 taaagaggca gaagagaaat ttaaagaagc tactgaagca tatgaggtat tacgcgatcc
     5641 aaagcgccgg gcacagtatg ataagtttgg gcatgaaggt gttgcaggat ttgaaggctt
     5701 tggcagagga gcatataccg atttttcgga tatctttggt gattttgata taggtgatat
     5761 atttgaaagc ttttttggtg ctggttctgg ttctggcaca cgaacacgat catcccgtac
     5821 gcgtcgggga agagatattc aatatgattt gtcaataaca ctggacgatg cagccaatgg
     5881 taaagaggtc cagatcgaaa ttccacgcca tgaggtatgc agtagttgtg gtggtaccgg
     5941 ttcggcatca ggttcaaaac caacggtatg tcctgtttgt aatgggacag ggcaaatacg
     6001 tcagacgcag ggattttttt cagtaacaca gacatgttat aaatgcagag gggaaggaaa
     6061 aattataacc tcaccatgca gggcatgtag aggtacaggg ttagaagaaa aacatcgcaa
     6121 aattacggta aagattcctg caggtgttga gtccggatct cgtttaaaaa tttccgggga
     6181 aggcgagcag ggtcccggag gtggttcggc aggcgattta tatgttgtag tacatattca
     6241 gaaacatccg ttgtttgaac gtcatggaaa tgatctgatt tgtgttgttg atataccgtt
     6301 tactatagcc tgtctgggag gtgagataga ggttccaaca atatacaata aaaaagcaaa
     6361 aatgaaaata ccagcaggga ctgaaaatgg tcaaatattc agacttaaag gcaatggaat
     6421 gccatatctg ggttcgtacg gcaagggtga tcaattggta aaaattaata tcaaggtacc
     6481 aaaaaaatta acacctcgtc agaaagaatt attaaaggaa tttgctcgca ttgaagggga
     6541 aacggtagat actgatagag aattttactc ttaaaagagc ggtgtttaca gccgctcttt
     6601 tacatattta gagattacat tttttgccgc ttctgacatc tgaacaaatt tgaaggtata
     6661 gtaaataatt ttttctctgg ttattgtccg tgaggcaata atggtgctga gtacttcaat
     6721 tttaaaattt tcaagtgtaa aaaatagtat atattctttt tgatattcca gccgtttctc
     6781 aaccctgatt acagcttcat actcatttaa gcgctctata gttccattta ttgaaacggt
     6841 ttcatcatgt tgtattatta cttcttcact atttttgctt tcatcgtttt tgagctgaac
     6901 agtgcaggga ataagtgttt gaatatatgg atgatgtacc tctttgcctc ttttaaatgc
     6961 ttgtggcttt tgtaatacta tggtattttc ctgaatggct atgatttttg aatcaaaaga
     7021 atattcggca tctccatttc taaaaaagaa aattgttact tgctctgcaa tgatgtcagc
     7081 agggatatcc tcaggtttac gaaaatattt acaatggatg gcatctccag taatctgtgt
     7141 tatcttagct ataaatgccc ttcctgttgc actatagccg cataccatgg tattcgtagt
     7201 caattcatcc ggtgaattta attgctcctg atattccacc gggttatata tcctttcata
     7261 ggttataact atatctttaa aaatatcttt taaatcttca ttgaatgtat ctcttgactt
     7321 ttttatatac tgtaaaaatt ttaccagacc tgtttcaaaa atggttacat tgttaaaaat
     7381 aatttcggga gtgcttaatt tagacagtga tatgatgtgt tgaagaatac gaatctgata
     7441 ccctgttaat cccctctggc gcgctttata gataaaaagt tgttcaatac ttgatttttt
     7501 taatttgaga tattggttat atcgcagcag tgcaaaaaaa cctatgatta caaggataac
     7561 aaataatgtt acaagcacct cccacagggg agctacccgc caccgtactc caaatactgt
     7621 aattatttcc atactggccg cataccatta aattaacccg cgtgtgccgc agtggtatga
     7681 atatttgagc tccctttgta aaggtggatt ctcgcatacg gacatctatc tacttcataa
     7741 tgaagcgtga tatcaatccg cagattagag attccctgct cagtagtatt ggaactacaa
     7801 tatgacatac catacacgaa caccgcaggc ttactgtcca atatactgaa ataatattta
     7861 ttggcaacaa aaattttact tgctaatgtg agtgaaaaca ctaatacagt gtggagcatg
     7921 gtttcataat aatagctgat atacagtaac tatcgaacac aaaaaagctt taaaggctac
     7981 atccatagtg ttgaaaaaat ttttcaaaca taaaatttgt tatagtgcaa tactatgtgt
     8041 catcataacc gttcctttat ttgtataccc atttggcaaa aacaaggtta cggttgaggt
     8101 atttacgtgg catattttaa agacaataca tttcaatgta tattatccct acggcatgga
     8161 aaagctggct gcaacgacgg caaccatcgc tgaaagggca tacggatatc ttgctgatac
     8221 actgcaacac cagctcaatg aaccggtacc aatcattgta ttcccttcgc acatagattt
     8281 tcaggaaaat aatatcctga tgcagatcat tggcgagggt actggcggat ttactgaagc
     8341 atttaaaaac agggtggtga taccgtttag tggttcgtac gatgaatatc agcatgttct
     8401 ggttcatgag cttgttcatg cgtttcagtt caatattctt atgcacgata gttccggtaa
     8461 actacagtcg atgatgagta tgggtgctgt gccattgtgg tttaccgaag gtatgtcaga
     8521 gtatctatca attggatttg atgaaactgc cgatatgacc atgcgtgatt tcttttttaa
     8581 tgatcagtat gtaagcctga tggactttac tacctatcgt acaggtaatt tgtactacta
     8641 ctataaagtg gggcaggcat tcttttactt ttttgaaacg gtgtatggca agggaaaaat
     8701 tggcgaattt ttgcgtgaca tacgcgatat gggtaacttt gaaaaggcat taaaggtaca
     8761 tacaaaaaag gatcttgagg ctattgataa ggaattcagg cacttttata aaaaaagata
     8821 ttaccctttg atggttggca gaaattttga tgaagaggaa ggtgaaaggg taaccgatca
     8881 taaaaaaacg cgttcagtgt tcaatacatg ccctgccata tcgcctgatg gtaaaaaaat
     8941 tgcctatatc gataacagag atatctatgc ttcaatttcg gttattacca ttggcgaaaa
     9001 agaggctgag gaaaaagccc gtgtgctatt gaaagggaat gaaacctctc agtttgaagg
     9061 aatgcatttg ctggataact atctgtcatg gacaccggat ggcaaaggca ttgtgtttgt
     9121 ttcacaatcc aacaacaggg atgttatctt tatactggat agtcgaaacg gtacaattat
     9181 caaaaagata gtactgccat ttcgttcgat taaagatcct gccttatcac ctgatggcaa
     9241 ctatataagc tttatagggc agaccaatac gcagagtgat gtgtatatct acaggctcaa
     9301 cgatacaatg ttacagcaga taaccaatga tgtgtatacg gaacggtatc ccaggcttga
     9361 taaaaaaaat gcttttattt actattcatc aaattatcca actaatgatt atacaggtac
     9421 cgactatgca attataagac ataatgtgct aacaggtgag cgacaggtta tagtgaaggc
     9481 tcagtcaaaa aatttacagg ttgatgttgc aggggatggc aatactattg tatatatctc
     9541 aaacagggat ggaatctata atgtttatcg ttatactatt gcaacgggcg aggaaataaa
     9601 aattactacg ggaacaaccg gtatatttta tccgcgtctt tttccggagg gcaatgcatt
     9661 tgcgtttgtt tcatatcaaa atggtggata cgatatcttt ataaaaaatg aaataaaacc
     9721 tgtaacagaa accccgctgt ttaacagtga gcatataccg gtgatgctgc ccccaaacta
     9781 tttcccgcta tcaaaagctg tgtgggacag ctatgaaaca aggatttcat ctgactgggt
     9841 ttttgttggg cttggtggaa cggttggcta tggctttact ggttttgctc agatgagctt
     9901 cagcgattat ctgggcgata accggattat tgtaacaact gattttttgc gggatgatga
     9961 gagcaacaac tttatgaatt acgatgtgca atatctttat ttacgatatc gatgggattg
    10021 gacctttggc tgtttcagac aaaaaagtcc ctatggcata tttacgctgg caaaccttaa
    10081 cgatattatt cataatgcat actggtcaac gctttcaatg gatcattacg gtgcctatgt
    10141 gattgcaagc tatcctttta ctaaattttc gcgtttcagc acccgtgttt cctcaagccg
    10201 ctatgaacag gattacggct atagtgatga acgtccggat gtttatggca atttaaattc
    10261 aatttctgta tcatacaatt acgataatgt tttgtggggt tttttagcgc cattgcgggg
    10321 cactcgtggg cagataatgt tgacccaggt gtttaacctg acggggcagg attattcatt
    10381 taccagcata gatattgacc tgcggcatta ttttttcatt gataaacagt atgttatagc
    10441 gctccgcggt gttggtggca agattatggg aagtgagaaa gagtatttta aatattatat
    10501 tggtggattt aatacccttc gcggacatcc gtttgtagaa tatggtggta gcaatgtttt
    10561 tttgttcaac gctgagttca ggtttacatt cattgaatca ataaacatgg gctggccact
    10621 ctttttaaag attggaaata tcggcggagt tttatttatc gatacaggtt cagcatgggg
    10681 taattcgtat acttttttta ataaagaaac agatcgattt gaagatttta aaatggacat
    10741 gggatttgga ttcaggctta ctgtgtaccc aatagttatt ttaaaacttg attatgcgtg
    10801 gccatattat tatggtaagt ttggtgaaaa agagatactc tttagtttag gatatgaatt
    10861 ttgatggggg aaagattcta ttttcaagga gtttaatgca atggaacgaa gtgcacatat
    10921 agaacgtaaa acaaatgaaa ctgacataac attaaaatgt atacttgata gtggtgatgc
    10981 aagcatcatc actacaggaa ttggtttttt taatcatatg ctcacattgt ttgttacgca
    11041 tggaaggatg aatatacagc tcaatgcaag gggtgatctg gaggttgatg atcatcatac
    11101 tgttgaggat attgggatat gcatgggaaa ggcgttcaat aaagcattgg gcgataaaag
    11161 cgggattagc aggtttgggc aggcagtcat ccctatggat gaggcttgca ctgctgctgt
    11221 tgttgatata tcagggaggg ggtgctttgt atataaaggc gataaactta ccggggcaat
    11281 agggcaatac caggaagaac ttacactgga attttttcaa tcgtttgcac ataatgccct
    11341 gattacactg catatacaac agatgtatgg cgacaaccgt catcatatac acgaatcaat
    11401 ctttaaatca gcaggattgg cactataccg ggcttacagc ttaagtggtc gcaccggtat
    11461 accatcaaca aaaggagtgt tatgattacc gtcattgatt atggcatggg caacctgcgc
    11521 tcggttgtaa aagctgtaga aaaatatacc agtgatgtac aggttagctc tgaccctgaa
    11581 agcatagcct catcaaatgc gttgataatg ccaggagatg gtgcttttgg tcaggcaatg
    11641 gaaaatctga cacaatcagg ctggattgaa ccattggtac ggtttatagg taatggcggt
    11701 tacttttttg gcatatgtct tggctatcag cttttgttta ccagcagtga agagtttggc
    11761 aaccataagg ggcttgacat tatacccggt actgtgatac gattcccgga tgatctaaag
    11821 gtgcctcata tgggctggaa tcaggttgaa ctaataaaag aacatccagt ggttaatggt
    11881 ataccgcaaa atagttattt ttattttatt cacacctact atccggttat tggcgatagt
    11941 tcctggataa tgggccaaac tgcctatgga gtgacattcc ccagcattgt ggggagggga
    12001 aattgtattg ccacgcaatt tcatccggaa aaaagccacc ggtatggact caccatgatt
    12061 gaaaactttg tgaggatggt atgctcgtaa tcccggcaat cgatttaaaa gaaggcagat
    12121 gtgtgcgcct ggtgcagggt gacccattgc aggaaaaggt gtattcgtat gacccggtaa
    12181 ctatcgcccg gcaatttgaa gatgctggag cacagcttat tcatgttgtt gatcttgatg
    12241 gagcatttga agggcagcct gtcaacatgc atattgtaaa ggagattgca aaatcggtat
    12301 ctattcctat tgaaattggt ggtggtatcc gtaccgaagc tgctattaaa atgtatcttg
    12361 atgagggaat agagcgtata ataatgggta ccatccttat tgaagaatcg tttagagaaa
    12421 tgattcaaag gtttaaagag tatattatag caggtgttga tgcaagggat ttacatgttg
    12481 caacacatgg gtggaaaaaa attattgaca taaatgccat tgaatttata aaggatattc
    12541 aaaacattgg tattgcagag attatttata cagatatagc tactgatggt atgttgcagg
    12601 gaccaaattt tcgatcgata gaaagaatat tagctgatgt cccgggagtt tcgttaattg
    12661 cctctggtgg tatttctaca atgcaggaca tacaaaaatt gttgcattat tcgggtagcg
    12721 gattaaaggg ttgcatagtt ggtaaagcta tatatgatgg gatggtagat ttacgcagtg
    12781 caataaagct gtgtagttag atggtggcaa tatgaatgat gatgtatcat tgtatggtga
    12841 ggaaataaaa ttccctgctg agattgagca atatgagcgc aaaatttatg atttaaagca
    12901 gcttattgaa ataagcaagg gattaaattc cactctggac tataatatac ttattgattc
    12961 catactttta acctgtatgg gacagatgca gcttttaaaa gctggaattt ttactcatcg
    13021 cagtattgac cgtcaggaat ttgtattgca tagaaattat aaaggttttg aaattgatca
    13081 taaaattgaa tatttaatac ctgccaattc aaaattaatt cagttccttg atatgaattt
    13141 caagtgttac acactggatg aactgaaaga tatattacgg gatgaacctt cgctggaaag
    13201 ccttatttgc cttgatcctt cactgatagt tccgttaaaa ggaaaaggta cccttaatgg
    13261 aattattata ttagctgaca gaataaatca aaaaggtttt agcgaatctg acaaagaata
    13321 tatgctcaac attgcttcgc tggctggtat agctattcag aacgcttatt tatatgaatt
    13381 agccacaact gatatgatga caaggcttaa aattcatcat tatttccaga ccgcattgat
    13441 ggaggaacgc gaaaaatcaa taaaaagcag ccttcctctt tcactgataa tgattgatat
    13501 tgataatttt aaagatttta acgataccta cgggcatacg tgtggtgatt tagttctaaa
    13561 gaatgtagca atggtgatat ctggttcggt acgacagagt gatattgcat cacggtatgg
    13621 cggcgaagaa tttgctgtaa tattgccgca tactgaaatc aatgaagcct ttaaggttgc
    13681 agagcgtgtg cgaacctcag tgcagaagtc atatattctc tataaaagaa aaaaattagg
    13741 tgttactgta tcagtaggcg ttacccagtt taatccgtct ctggattcaa cctcaaaaaa
    13801 tattattgaa cgcgctgata aagcactcta tatgtcaaaa caacagggta gaaacaaaac
    13861 aactgtcctc cttacggata aataaaaaat aaaataaaat gcttgatttt gtcttgtatc
    13921 actatataat ttcctgccat aatacgaaaa tagtaataag tgnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnattatatt aagttcatat cgggtatgtt aatactaacg
    14101 ctatctatta attttattat ttatccagta atggcgcaaa ctattgaaga agatgccggg
    14161 aagaaatata aggggactcc ctatcaggat gaatttgata aaaaggcaaa agaacgcagg
    14221 gaagaacctg cgatgatgga gcgagatcca cgattggcgt gtttattatc attgattatt
    14281 cccggtggcg ggcatatcta tttacggaat gatttgaagg ctgtaggaat ttttttgctt
    14341 acagtaaccg cttattcatt ttctgcatac tatttgtatg tagctcttaa taaaaccgat
    14401 tcatcaacag aaaaaaagtc aaaactcata ttatcaggac ttttatttgt agtgggagct
    14461 attattcatg tggtgggcat agttgaggca tataatgatg ctattgaaat aaatgaacag
    14521 caatactatt ttggggctga aagatctcca tgtccttata ttgctaagaa gtagctactt
    14581 tatgggaatc aaaatcttta ttaatacctt aaagtaatac ttttgcatca gcatgcagac
    14641 catcaatcta taaggcttct aagaattttt tgtatgtata gttgtcccca ctctcgttat
    14701 ctgatgggtt tattattttg tggtataaat ttgtttgtga tgtgttcccc aaataatgat
    14761 ttgcctactt aactaagata tagtcaatta tttaccatga atgatgtttg ttttccttta
    14821 tctatatatt taaataatta acagtgataa taagtgtcaa tgctgattta ccttcataaa
    14881 atgttaaaat agagtaaatg gataatgatt ttaaaataaa ttgtaaaaat aataccatta
    14941 taaacaattt gtcattgaaa atgtactata aaaaagctat agtatagtac aatgatatac
    15001 ccattaaaca ctttttaaat aattgaaatt tattttcttg acatgtagta tagtatatta
    15061 ttattttgtc ttttcacaag gggcccatag ctcagcaggt tagagcggct gactcataat
    15121 cagtaggtcc ggggttcgag tccctgtggg cccaagagta gagctaaatc ctctattatt
    15181 gttttttctg tatttaataa tataagattg caataaagtg gtaaattgaa aaatcgtgga
    15241 gaggtgtatg tacgcaatag ttgaaatagc cgggaaacaa tataaggttg aaaaggataa
    15301 aactgtagct gttgacaaat tggatgcaaa ggataataat gaaatcgcta tcgacaaagt
    15361 tataatgttt gttgatggtg agaaagtact tattgggcaa ccgtatttat ccaacgttgt
    15421 ggtaaaggca aaagttgatg gaatggttcg gggtaaaaag gtgcgcggaa taaaattcaa
    15481 gaggcgcaaa aattatacta gaacatttgg gcataagcag cactatacaa gtataactat
    15541 tgttgatatg tctgtgcagt agtgattaat gtcactttac aggggattat tctacgtgac
    15601 ggaaacattg cattatccgg cagcgaaatt gttataacga ttacgggaca ttctacaaaa
    15661 gctgcaaagg gcaatgatat tgtttgtgct ggtgtttcag cattagcgca atccgtagta
    15721 gttgctctgg agtattgggc gatagttcat agtattgttc aggatgatgg attgttacgg
    15781 ttttcaatac atgttccttt attaaatgac agggagataa cccgctgcga aagtatagta
    15841 agcgttttag ttattggttt aaatgaaata cgaaaacaat attcacaatt tttaactatt
    15901 aatgttaacg agtaaatttt gatactattt tgataattat tgtaaataag aggtgaatac
    15961 atggcacata aaaaaggtgg tggttcatca cgaaatggtc gtgattctca tgctaaacgg
    16021 ttgggaataa aagtatctgg tggccagttt gtaaagagtg gctcaataat tatacgacaa
    16081 agaggaacaa aacttcatcc cggtttaaat gtaaagcgcg gcagtgacga tacactcttt
    16141 gcggcggctg atggtgtaat aaaatttgaa cggcttggta aagaacggaa gaaggtttcg
    16201 gtatatcctg tacaatcata attacgttac cagtgtaatt gtaattgtat attaaaaagc
    16261 tattgaataa agttattgaa tgaataataa aaggggtaag tttttaagct gtggcccctt
    16321 tttctttttt gtacatactg ctcaatggaa tagtgtttgc agtaaactcc tttgcattgc
    16381 ataaatctcc ttactctatc gttgaaactg ctccctcaag ggtaaacatt atcatgttgt
    16441 gacgaagaaa aagtgaaaat ggggtagcct ataaactcaa ttttagaaaa atacatcaat
    16501 tgcgatacta tcagtaatat aacaaataat actatagtaa catagtgatt aataaataca
    16561 tgtgcagtat taagtgctgt ccttttatgc tattatctat caaaaacgta aggaatgatt
    16621 tgtggctaaa tttaccgata ctataacgat aaaaattaag gctggcaatg gcggtgcagg
    16681 tgctgtttcg ttcaggcgtg agaaatatat tccaaaaggt ggtcctgatg gaggcgatgg
    16741 cgggaaaggc ggtgatgtca ttatacaggc acagcaggga tactataatt tagggcacct
    16801 gtttaaggat agaatatata aagcaaaaaa tggcggatat ggcatgggac aaaaccgaca
    16861 tggcaaagat gggcaggatt taattattaa agtgcctgca ggtactgaag tttttgatac
    16921 acaaacaggt gagcccttag ctgaccttgt caatgaaggc gatagtgtat gcgttgcaaa
    16981 gggtggcatt ggtgggaaag gaaatgcatt ttttaaaaca tctacccatc aaacaccgcg
    17041 atttgctcaa cctggtatgc agggtgatga gcgtacggta acgttaaacc ttaaattaat
    17101 agctgatgtt gggctggttg ggcttcccaa tgttgggaaa tctacgttgc ttgcaaaaat
    17161 aactaatgct cagccaaaaa ttgccaatta tccttttacc acgcttatac ccaatttagg
    17221 ggtcattgag cagggagaga aggttattac gatagctgat attcccggga ttattgaagg
    17281 tgctcataaa gggttgggat tgggtctttc atttttgcag catattgaac gggttaaggt
    17341 aattttgttt tgtgttgaag ctgttgatgc agacccatta tatacacttt cactgttaaa
    17401 agcagagtta tcaacgtata atgataattt gtcaaaacgg cattcaatac tactgctgac
    17461 aaaaagtgat atggtaagcg aatctatttt gcaaacaagg atacaactat tgcaaaaaga
    17521 aaaatatgat tgtgtaccca tatcatcatt gacaggatat aatattgata tattaattga
    17581 gaaacttttt ttgataatgg aataaacaca tgccacgttc tcagtattta aaacgggtaa
    17641 agactattgt tgttaaggtt ggctcatcgc tcattaccaa ttcagatggt atatctgctg
    17701 aatgcgttga taagcttgtt gacgatgttg tttttcttat taaaaaaaag tataaagtag
    17761 tgattgtaag ttcaggtgca atcagtgctg gttcttctgt tatgaaaaaa aaacgcatgc
    17821 tgtgcactat cccggaaaag caggcgttag ctgctattgg gcaatcaata cttatggatg
    17881 tttaccgggg atgttttaat aaacgcggat atcaggttgg acagattttg ctcaccgaag
    17941 atgatgtaaa acatcgcagg agatttttaa atgctagaca taccttgaat gctcttctgg
    18001 atatgggagt tgtaccgata gttaatgaaa atgattctgt tgtcattaaa gagattaaat
    18061 ttggtgataa tgatacactc tcagcgcatg ttgcaaatat tgtacaggct gatctattga
    18121 tattgttgtc ggatgttgat ggattttact gggatgagca tgatgaagaa ccggtgagtg
    18181 agatttttga aataaatgat gaagtaaaaa aacgatgccg tggtgctggt agtgagcatg
    18241 gggtaggtgg catggttaca aaaattaaag caggcgaaat agttataaaa tcaggcgtga
    18301 tgatgataat tgccaatggg aaaactgaaa atatattaaa gcaaataatg cagggcaaaa
    18361 aaataggcac gctcttttca ggaaaaacac agttaaaaag caaaaaacgc tggatagcat
    18421 tcaatgttaa accaaaaggt aagcttacta tagatgatgg tgccgtgaag gcattgaaag
    18481 aaaataaaaa atcgctactt gccataggga taatgaatgt tgaaggggat tttgatctgg
    18541 gcgatgcagt tgagatagtt gatactcatg gaatggttat tggcaaaggt attgttaatt
    18601 ataccagtga tgaattacat aaaataaaag gcaagaatac tcaggaaata aaaaagatat
    18661 ttggttcaaa atattatgaa gaggtcataa acagggacga tttaattgtg ttttaacaat
    18721 aggttcttta ttgtgtaacc ttaaaataaa atttgtttca aatagcgcat ggtaatcgta
    18781 tatacattga catatatcag gtttgtacaa taatgatgaa aatggtgaag ttgattggta
    18841 aaagttaaat cattgaggta tcccctcctc aatggaaaat attataacag gataatggta
    18901 tgataaatac aatattaagc cttttctttg gaacaaagca tgagcgtgat attaaaaaac
    18961 tgaaaccaat agtacaacaa ataaatgcgc ttgaagacga agtgaaaaag cttacccatg
    19021 atcagatgct tgcaaaaact gctgagttca gaggacgcat tgaaaatgga cagccggtag
    19081 atgatttatt gccgtatgcc tttgccatgg tcagggaggc tgcatggcgc actattggta
    19141 tgcgacattt tgatgtacag cttatgggag caatcgtact gcatcagggt aggatttctg
    19201 agatgaagac cggtgaagga aaaacgctgg ttgcaaccat gccggtgtac ctgaatgcat
    19261 tggctggtaa gggtgtgcat gttgtaacag ttaatgatta tttagcacgt cgtgactctg
    19321 agtggatggg tccaatctat aagtatttag ggcttacggt tggagtgatt cagcataaca
    19381 tggattacta tcagcggcaa cagtcatatc gatgtgacgt tacctacggg accaataatg
    19441 aatttggttt tgattatttg cgcgataata tggttgagca tcgttcattg cgagttcaac
    19501 gtgaactgta ttatgccatt gttgatgagg ttgattcaat tctgattgat gaagcgcgta
    19561 ctccgcttat tatatcaggc tctactgatg aatctacaaa aaaatattat ttgattaata
    19621 aaataatacc aggcctcagg gaaggtgttg attatgaagt aaatgaaaaa gatcgcaact
    19681 gccttttaac cgaagagggt gttcgccatg ttgaacggct tttaaagata gataatctat
    19741 acgatagtcg caacatagaa ttagttcacc atgttaatca ggcattgaag gcgcatacgc
    19801 tgtttaaacg tgatattgat tatattgtga aagatggaca ggttgtcatt gttgatgagt
    19861 ttaccgggcg ccttatgcca ggacgacgat attctgacgg tttgcatcag gcactggaag
    19921 caaaggaaaa tgtaaccata gcacgtgaaa gccagacatt tgcacagatt acctttcaga
    19981 attacttcag gatgtataaa aaattagcag gtatgacagg tactgctgat actgaagcca
    20041 tagaatttaa aaagatatat aaccttgatg ttgtggtcat cccaacaaat gaaccggtac
    20101 gcagaacgga ttatccagac aggatatatc gcactgaaaa agaaaagttc aacgctattg
    20161 taaaggagat tcaacaattg cgtgaagatg gcagacctgt gctggtgggt acaatatcga
    20221 tagaaaaatc cgagaagctt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnttttacct ttcacttgaa gatgatctta tgcggatttt tggttcagag cgcattgcag
    20401 gtgtcatgca gcgccttggc atggaggaag gacaggaaat tgaaagcagg atggtatcac
    20461 gagccattga aaatgctcag aaacgcgttg aaagcagaaa ctttgagatt cgtaagcatc
    20521 tgcttgaata tgatgatgtt atgaatagcc agcgcgaata tgtgtataaa gagcgaaacg
    20581 caatacttga tggcgaagat gtgtccatta agattaagca atatattggt gatgttgttg
    20641 aaagctgggt gccgtatttt actcaaggca aacgaaatgc tgaagattgg gatatggagg
    20701 gattaaacgg ctggttgtat tcaaaatttg gttttgattt acagtcaatt gcaaaagatg
    20761 gtgcgcgcct ttcacatgat gagattatag atggtattac caataaattg atgcaattat
    20821 acagtgctaa agaggaggag ataggcagcg agcatatgcg tacgctggag cgtttggtat
    20881 ccctgcaggt aatagacgct aaatggcgtg atcacctgct ggtaatggat gatctgcgtg
    20941 atggcatatg gactgcaggg tatagcgagc gtaatcctct ggttgaatat aaattgcgcg
    21001 gttttgaact ttttaatgag atgctggata accttaaatt agatattatt gagatgcttt
    21061 taaaggtgcg tttacaaaaa gttgaggaaa ttgcaataca accggaaaag attggaatcc
    21121 cgctgggtca ggaatatcat ccagaagttg agcagtttgg tgctggcggt atacccgttg
    21181 cgacaccacc cgtaacaata gcctcgcatg gtgatgataa aacaagagag gtgactggcg
    21241 gggtaaggcg taaaaagaca cgaagaagca ggcggggata ataatgagcc ttatgaaaag
    21301 catctccggt atcagaggta ttgttggtca gtctctgacg cctgatttga ttacaaagat
    21361 agcaatggct ttttccagac atataggtgg cggcacagta gttgttggca gggattcgcg
    21421 cggtagcggc ggtgccattg ccggatgttt tgatgctgtg ttgtcgttat gtggatgtaa
    21481 tgttattgat attggcattg ttcccacccc aaccgtgcag attatggtag aacatatgaa
    21541 ggcaaaaggt ggcgtggtaa tctcagcgtc acataatccc atacagtgga atgcctttaa
    21601 attgattaac aaatcgggaa gtttttttgg tcctgacgag atgaatgcct ttttacagct
    21661 tgttgatgag gtttcgccgt ttggtgtagc atggaataca attaaaccaa tacagtgtga
    21721 tgatactgcc atggatatac acatccagaa ggttttaaca gctattgatg ttgatgctat
    21781 ccgaaaaaaa catttcaagg ttgtgcttga ttcggttaat ggtgcaggtt ccatcattac
    21841 accagagctg ctgaaacatt taggctgcag ggttattccc atacactgtg atatgcagtc
    21901 tggagttttt ccacgcggtg cagaacccct tgctaaaaac ttaaagcaat tggccaatac
    21961 cgttaaacag cataaggcag atattgggtt tgctcaggat ccggatgctg acaggctggc
    22021 gatagttaat gaacatggaa aacctattgg tgaggagctt acggtaacac tggcagtcat
    22081 gcgatgcctt ttgaagaaaa aaggctctgt tgtggttaat atgtcaacca ccagagcagt
    22141 tgaggatgtt gctcatagtt ttggtgctac cgtatatcgc tcaaaggttg gtgagattca
    22201 tgttgttgag gcaatgaaga agcacagggc agttatagga ggggagggca acggcggggt
    22261 gattgcacca gaggttcatt atgggcgcga cagccttgtg gggattggtt attgccttga
    22321 gcttatggct ttgagcaatc gatcgatttc agagattgta tcatccctac cagtatatta
    22381 catgataaag gataccgttg ctattgagca gggaattgat actggagcta tcgcacatga
    22441 aattgccagt cagtaccacg atgaaatgat caatacgctt gatggaatac gtattgattt
    22501 tattcatcac aaccgcttta aaggcgggtg ggtgcactta cgagcttcca acacagaacc
    22561 aattttcagg attattgccg aaggtacaac aaaagagcag tgcacggcaa tgattgatgc
    22621 gtttaaaacc atgaagatag cgccataatt ataattactt gacaaaaaat tgccttactc
    22681 cgtaatacta tcgcattcaa ttttattacc tattattttg ttatttttga attttatatt
    22741 gattgtggta ttgtgcaata ttattttata gtgggttgga aaaatgttta acgataggag
    22801 ctattgtggc gaattgacgc ttgatgatgc aggtaaacag gttacactgg ctggttgggt
    22861 tgataccaag cgtgatttag gcggcataat ttttattgag atgagggatg ttacgggtat
    22921 tattcaggta gtttttgatg tgtcaaaaaa tggtgaagca taccagatag cggatagagc
    22981 aaaaagtgag tactgcatct atacgtgcgg ggttgtttca aagcgaactg aagataccat
    23041 aaatcccgat cttaaaacag gtatgattga ggttgtagct catgaggttg taatcttatc
    23101 cgaatcagag gtgcctcctt tcccaattga tgtgtatacc aaccttaatg aagatgtcag
    23161 gttgaaatat agatttttag atttacgaag ggccgaaatg caacaggcga tagttattcg
    23221 gcataaggtt atgcaggcta cacggaatta tctttccagc aatagatttt ttgaaataga
    23281 aacaccaatt ttaaataaat caacgccgga aggtgcacgt gatttccttg tgccaagccg
    23341 catcaaccgc ggaatgtttt atgcattgcc gcaatccccg cagctattca agcagatttt
    23401 gatgatagca ggttttgacc gctactttca gattgccaga tgtttccgtg atgaagattt
    23461 acgcaatgac cggcagcctg aatttactca gattgactgc gagctttcat ttattacccc
    23521 gcagattatt atgcaggtta ttgaagggtt gatcaaagat atggtaaagg tgcttggcta
    23581 cgatatacat acgccatttc cagtaatgac ctatgatgag gctatgcagc ggtacggtaa
    23641 agatgcacct gatacacggt ttgggcttga gcttgttgat gtaagcgata tctttgccgg
    23701 ctcttcattt aatgtgttta aggcttcgct tgataacggc ggtattatta aggcgatagt
    23761 tgttaatgat gaaggtaaaa tttcgcgtgc catgattgat gagtacatta actatgtacg
    23821 gacatttaag gcgcatggtt tacccagcgt acgctatacc ggcagtaact ttgagacggg
    23881 aatagcaaaa tttttaagtg atagcgaaaa gcaaaagctt attgacaggc tttcgttaga
    23941 ttcaaattcg cttattattt tttcgtgcga caaacaaaaa gttgtcaatg atgctctggg
    24001 aaatttaagg cttaaaatag cacagcagct cactatgatt gacgacaata aacttaactt
    24061 tttatgggtt gttgattttc cactgcttga gtataatgct gaagagggca gattttatgc
    24121 catgcatcac ccttttactg ctccaaaacc tgagtgcgaa gcaatgctgg actcgttaac
    24181 accggataat gttgatatgg tcaaagctca ggcgtatgat gttgtcctta atggtgttga
    24241 gattggtggt ggttccatac gaattaaccg ccccatggtg cagaagaaga tgtttggcat
    24301 attggggata tcagaagagg acgctaaaat taaattttca tttttacttg atgcattgaa
    24361 gtatggtgcc ccaccccatg gcggtatagc actgggattg gatagaattt taatgatttt
    24421 gttaaaacga aattccatac gtgatgtcat tgcatttcca aagactcaga aagggcagtg
    24481 cctgatgagc gaagctccct cgccagtgag ccccgagcag ttaaaggagc tatccattaa
    24541 ggtggttgag gtataatgga aaacacaaag tttgaaattg atgatatatc aatctataag
    24601 cgtatatgtg gctataagga taaaaatata aaaacaattg aaaaacttct ggatgtaaag
    24661 attatcccgc gtggcaatac attaattgtt gaagccttaa atgacaatgc agtaaaagcg
    24721 ttgcagttgc ttcattgcat gggtgattat ctgtatatgc gcgactcatt atatgaattt
    24781 gatgattttg acattcgcta tctggctctt tccattaatc agggtataaa aattgatgct
    24841 gacgagatca acaggttaaa aatttttata cctgaaacag gcaagacaat tttacccaaa
    24901 acaataaatc aagctcatta tatgtcggct atccataaaa gcccaataac catttcaata
    24961 ggccctgccg gtaccggtaa aacctattta gcagtagcgg ttggtgttaa atttttatta
    25021 acaggaaggg tggagcgcat catattaaca cgtccggcag tagaagctgg tgaaagcctt
    25081 ggatacctgc ccggtgattt tatccagaaa attaatccgt atctgcgtcc actgtatgat
    25141 gctctctttg atatccttcc ctacgagcga atatcaaaat atatggaaaa ggggattatt
    25201 gagattgcac cactggcata tatgcgcgga agaacgctga ataaggcatt tgttattctt
    25261 gatgaggctc agaataccac ctcctcgcag atgaagatgt ttttaacacg gttgggaaac
    25321 aattcaaaga tggttatatc gggcgatatt acccagattg atctggaaaa accaaaacaa
    25381 tcaggtttac tggctgcgca aaaaatactg catggtatag atgagataac ctttattgaa
    25441 tttttaaaag aggatatctg cagacatcca attgttgaaa agataattgc agcgtatgaa
    25501 aaacataccg aatagtatta tctggttatt attatgctaa attcatttat ggcatttaaa
    25561 cggggcatag aatttgctct taaaaagaaa agtataaggc tttcatttat atatactatt
    25621 gtggtcattg tatccgctac tgcgcttctt accatcaata gaacaggtgc tacctacaat
    25681 tatgaggctg gtgatatagc acgtgaggat atccgtgcac caatgaatat tgagtacccg
    25741 gttgctttag aaacggaaat agaaaaaaac cgtattgcgg aatccatgcc tgcggtattt
    25801 gatctcaatg agtcaattgt agaagataag cttaagatat gcaatatatt gtgtgaagct
    25861 gtcattaagg ttaagcagca gatgccggac accaaccctt ttaagcaggt tttattgctc
    25921 agaaccatgc tgcccgatta tctccagtat tcagataagg tattaattgg gctattgcaa
    25981 tatgatgagc agcttttaca tagagatatg aataaagtta ttttgcaggt ttacaataat
    26041 ggaattcttg aaaagcagta tacaaatccc cttaagctca aatcaaattt tatagtcatt
    26101 cgtgttattg gcaatgcttc agaattaaaa gaaaggcagg tcagtattga tgctctgtat
    26161 gacattaaca aaattaaaac attagtaccg gtaatatgta aagaagtatg taatcagccc
    26221 tcaaatgagg tcgtctatgc tatcagttcg gttgttatga gtttgttaca acctaacctt
    26281 ttttataatg aagaagaaac aaagaaaaga ataaccgatg ccattcaatc ggtaaaaccg
    26341 gtgatgcgtg taattaagaa aggacaatca attgcgcgtg aaggggatac aatcactgaa
    26401 gatgttttga aaaaaattga tattatcaat aaaaattcca ggttttcagg tttcagcttt
    26461 gtactgggat tacttcttac ccaattgggt tttctgatca tttttggcta ttttttaatt
    26521 acctattata ccaatctggt tgttgacaat aagatgccat tgattgtgtt tacccttgtc
    26581 atggcattta tcattattac tttttttatg gcacgatggc agggtagaat gtttgaaggg
    26641 cttttctttc ctcttctttt gccaatacca ctgataacaa tgatattggc tataatgtac
    26701 aatatccata ttgccatgtt aattgggatg tatattgtat tctttgtagc aattatcaca
    26761 aaaggtgatt ttacatcatt actgctggct ttcagttcgg cactttcagg agtgtttgtt
    26821 gtaggtgatg tagaacggcg atcagacttt ttacggggag gatttacttt aggacttatt
    26881 aactcggtga ttgttgtttc agttggttta atgaatgaat tatcatttgc tatgattctt
    26941 aataatatag ggcttgcgtt tgtttcagga attatcaatg cgatagttgt ttttggagta
    27001 ttcccaatct atgaaaacct ttttggttta acaacaaaat ttaaattgct ggaactatcc
    27061 gatcttaatg cacctatttt cagggagatg cttataaaag ctccgggaac atacaaccat
    27121 tcaatcatgg ttgcaacgct tgctgaatcc gcatgtaagg agattggagc caatgcgttg
    27181 cttgccaggg ttggaggata ttaccatgat ataggtaaaa ttcaggatgc aaacttttat
    27241 attgaaaata aggttaccga tccgcgcgct aaaaagttat ctgcccttga gtattgtaaa
    27301 ttgatcatct ctcatgttga gaagggagtt gagctggcgc gcaaaaataa tctacccgag
    27361 atggtaatag attttatccg tgaacatcat ggacaaacag taatgacata tttttaccat
    27421 caggcactgg aggagagtgg tgccagtgga gatattatcc agaaaagtga ttttcagtat
    27481 cccggtccta aaccgcatac acgggaaact gcagttgtta tgcttgcaga tgcaattgag
    27541 gctgctgcac gttctattca ggaaccaacc atcgaaaaat tagaatcgtt gataaaacga
    27601 ataatcaata accgtttgaa cgaaggtgat ctggatcatg ctgatattac catgaatgag
    27661 cttaataaga ttcagcattc catactgcgg gtattgcagg gaatatttca ctcacgcatt
    27721 gaatatccaa cagatgatga aattaataga ttagagcaac gatcagagca tggccattaa
    27781 cattactatt gaaagtgaag atatttcagt ttctaaatat ttaaaaacat tctgtaaaaa
    27841 aaacatcaag gctatactac ggtatctcca cattgataat gttgctgtaa cgcttgtgct
    27901 taccggtgat gcatcaattg cccgttttaa cagaaactat cgcaaaaaaa aaggaccaac
    27961 tgatgtgctt tcctttgcct accgcgaggc gccaacgcca cttgctgacg gtttgcctga
    28021 atatcttggt gatgtaatca tatcacttga aacagcacaa aaacaggcac aagaatataa
    28081 tgttaccctg catgacgaaa tgctgcggct tattgttcat ggtatgttgc atctgttggg
    28141 ctatgaccat gagcaatatt acaaaaaaaa acagatgcaa aaaaaagaag aagaactgct
    28201 gcagcattta cgttgataag caacaaaaat aattttagtc tatattcaca atagttttta
    28261 tggtgttcag cagctcatcg gctaattggt cgttgcaatg atttacctcc tgtgatgcct
    28321 gcttgatatc atcagcttgc ttgcatgagc taaaataaaa tttaatcttg ggctccgtac
    28381 ctgatggacg catggtaacc tttgatctat tttctagact atattgcaaa acatccgatg
    28441 gtgggagatt ggccggttta gtggctccgc ttgcaatgtc tacaattcct gctttgccgt
    28501 caaggatgga tacaaccttt ataccagcaa actgctgtgg agggttgctg cgaaaatgtt
    28561 tcatgatggt ttttattttt tctattcctt ccactccttt caaggtcagt gaaaccaatt
    28621 tttcattgta gtacccaaac ctgctataaa tttcatatag aaagtcgtta agcgttttgt
    28681 tttgtttatg cagccagtgt gtcatctctg caaagaaata gcatgagctt acggcatctt
    28741 tgtcgcgcac aaaatcaagg ggtaaataac catagctctc ttcaccgcca aagataaagg
    28801 tatgcgtatg ctcatagtcc cgtatcttct ttgcaatcca cttaaaaccg gtaagaacat
    28861 tttccattga tacatggaag taatcagcta tagcctgctg cagttcggtg gtaacaatgg
    28921 tcttaaccac aaaactgttg cggggcaatc tattgtgatg ctgccgctgt aataaaatat
    28981 aatacagcag catgctccct atctgattgc cgtttattaa tgtatactcg ccattggtat
    29041 ctttgaatgc aacacccata cggtcggcat cagggtcggt ggcaagtact atatcggcat
    29101 tcatggtttt agccaatgct attgccatgg taagcgcttc aggttcttcg ggatttggtg
    29161 atgcaaccgt tgggaagttg ccatccggaa ttgcctgctt ttccacaacg ttgacattgg
    29221 taaatccaaa tttttgtaaa atcatgggta caatgttgta gcctgttcca tgaagcggcg
    29281 taaaaacaat attgatgggc gatagttcct gtgatttgaa tataacaccg gaaagtttat
    29341 ctgcatagct attgattatt tcattgccag ccagttgtat caatttattt ccagcagcct
    29401 tgtgaaaatc catcttttta atttgcgtaa ttgcattaat tttttgcacc tcatcaatga
    29461 tctctttatc ctgcggagga acaacctgac cgccgtcgtt ccagtatact ttatagccgt
    29521 tatattcggg agggttgtgg cttgcggtta taacaatacc ggcggttgcc ttgaaatagc
    29581 gtatggcata cgagcagatg ggtgttggcg taatatcgct gaatagatat accatgatgc
    29641 cgttagcagc taaaatactg gctgcctcct ttgaaaaaac atccgacatc ctccgtgagt
    29701 cataggctat gacaacaccc tgttcagcgc tccctttttg taaaatgtaa ttggcaagtc
    29761 cctgcgttgc cataccaacg gtatagatat tcatcctgtt ggtaccggct ccaattatac
    29821 cccgtaaccc tcctgtacca aattctaaat tggtatagaa gcgttcatac agttcttttt
    29881 cattgttttg ttgtaccagt tgattaattt catgaatggt ttcactatca aacggtggct
    29941 gttgccattg ctgtatgcgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30061 tcggtgtctg ccgggaagga ttccatggca ttgctgcata ttctttttgt attgcgcgta
    30121 aggcttgatt gcacaattgc tatctatcat cttgaccata cagtgcgtgg tgatgaatca
    30181 tttgatgacc tgctgtttgt gatacagcag gcagcacggt atggtattgg tgcatttgtt
    30241 gaacggtata attttaacca gcataaagaa aaaggcaaaa gctttgagca gcaggcacgt
    30301 gaataccgct atgcccgttt acaaatggta gcacatgaat atggctacac aaagattgca
    30361 accgcccaca cctgtgatga tcagattgaa acattggtta tgcgaatatt tcagggaaca
    30421 gggctgcagg gattacaggc aataccgctt atcaataata ctatcatccg cccgcttttg
    30481 tcattgcatg cagacgatgt ctatgcatat tgtacctgta atgagctatc gtaccgtcat
    30541 gatttaacca atgatgatac gcactatctg cgaaacttta tacggcacga ggttatccct
    30601 ctggttaaaa aaaggttccc cacttttgat aaagcattgc tcaatttgca gcagcaggct
    30661 ctgggtgctg tcaaaagtat agagcagctt atggcaaagg tttatcctga ttgtatcaaa
    30721 cagggaggca atcatgttga ggtgcctgag gatgctctat ccctcaatga atacttattc
    30781 aaattttact gtgcctccat tatcagtaac tatctccaca ggtattgtga tacggctatg
    30841 ctggatgcgc tttacagggc acatgtatcg gataaaaaaa atgtaacgct gtttgagggt
    30901 aacggtatat ttgtttaccg gcgggtaaag caatcgggtg cagtgttgct gttttgctct
    30961 gaccccatta tgggcgatag taactatgaa tatacaattc cgctgggtga agatatttat
    31021 attgatgaat tgggtttaac cgtacgctgt atggttatgg agtcaacgga tgccatgcca
    31081 tgcacagaaa gtaaaacgat agtgttgcgc tacaccggat atggtgatat cgttgtcaga
    31141 aacagacgca gtggtgatac gattttccga aaagcaggaa ggcgcaccct gaagcgttta
    31201 tatattgatt ataagcttac gcctgaacaa aagcaaaagc taccaatcat tgtcgttgat
    31261 aactgtgtgg ctgcggtatt gtttgataca ataatttccc aaaaaccagc aatttcacaa
    31321 acttttttgc cgttgccgca tgaaaaaatg cttgttattc actattggta taatcataat
    31381 gattaaataa cactcactcg ttacgatgaa gtaacatcat ctgatgccct atatatattt
    31441 ttttctgtaa agtatatagt cacatcatac agaaaagtta tagggggtgg aataatttat
    31501 ttgagtattt ccataattgt agtatcttgt aagtgaatac tacaattgat actatgactt
    31561 cttaaacagt agtgataatt actacgggag attatgatga acaaagctgc aaaacaggtt
    31621 gctctacttc tgcttatcgt tgtattggct atagcaatat tcaggatgaa tgaagtaaat
    31681 tctcagaagg ttgagcaact acaatatagt gatttcataa agaaagtata cgaaaataag
    31741 gtaaaatcgg ttagcatcgt taacggtaaa aaaatagaag gcgcctatac caagggtgat
    31801 aaggtgtatc tctttgagac agttatcccc tatgctgatc ctgatttgat taaaacgctt
    31861 atcaagaata aggtagaggt taaaggcgaa gaggttgatg ataatttctt ctggaagggt
    31921 ttacttaatt tcctcccatg gttgctattt tttggattta tctggttttt catgatacgt
    31981 cagatacagt ctaccggtaa caaagctatg agttttggca aggtaagagc aaaattacaa
    32041 ccagaaacaa agaacaaggt aacctttgca gatgttgcag gtgtcgatga agcaaaagtt
    32101 gaactgcagg agattattga atttttaaaa gatcctaaaa aattcatcac aattggagca
    32161 aaaattccca aaggcgttct tctggttggc cctccgggaa cgggtaaaac attgctggca
    32221 cgtgcagttg ccggggaagc aggcgtacca tttttttcca tcagcggttc tgattttgta
    32281 gagatgtttg ttggtgttgg cgcttcgcga gttcgtgatc tttttgaaca gggtaaacgc
    32341 aatgctccct gtatcatttt tattgatgag atagatgctg ttggcagatt gcgtggagca
    32401 gggttgggtg gtggccatga tgaacgagaa caaacattaa atcaattact ggttgaaatg
    32461 gatggcttcg aaaccaatga aggtgtgatt attgttgcag caaccaaccg tcctgatgtt
    32521 cttgacccgg cattactgcg tccggggcga tttgatcgtc aggttgttgt ggatatacct
    32581 gatatcaaag gcagggaaaa gatactggct gttcatacgc gcaaaattcc tttggcaaag
    32641 gatgtagacc tcaaggttat tgctcgtggg acaccgggat ttaccggcgc tgatttagca
    32701 aatcttgtta atgaagcagc actgctggct gcgcgaagaa ataaaaagcg tgtaacaatg
    32761 cttgaaatgg aagatgctaa ggacaaggta ttgatgggac cagaacgtaa atcagtgctt
    32821 atttcagcag aggagaaaaa agtcattgca taccatgaag ccgggcatgc tattctgggt
    32881 atcctgacag gaactgaccc tattcataaa gttaccgtaa taccacgcgg cagggcgctg
    32941 ggattaacaa tgcatttgcc aaaagaggaa cgacatatcc ataataaagg atactggctt
    33001 gatcagataa ccatattgtt tggcggccat gtagccgaaa agataaaatt tggacaggtt
    33061 accacaggag caagcaatga tatagagcgt gccaccgata ttgcacggag gatggtttgt
    33121 gagtggggga tgagtgaaac gttaggcccc cttgcttttg gtaaaaaaag tgaacaaatc
    33181 tttcttgcac gggagatagc tcagcacaga gactacagcg agcaaacagc acagctcatt
    33241 gataaagaag tgcatgaaat agttacaagc tgccgtgata gagctattat attaattgaa
    33301 gaacatgctg acaaatttga actcatagca acaaacctta ttgaacgcga aacactgaat
    33361 gcacaggaaa ttgaactgtt aatgaaaggt gaaccgttgc cgctaattca taacggtgat
    33421 agtgaaccac ctgatgaaga tgagccaaag attggaaagg caaaaagaaa aactgtatcc
    33481 aaatcattgt tggataaaga aataattcca acatagatgt cattgaggtt agcatgattg
    33541 atgatgaatt ggacaggata ttgcaggaca ttgaacccga acaggattat ttaacaaaaa
    33601 aagtaaatgc cagtaaaaaa tatgaagaat atatacttga tgaaataacc gaagataaca
    33661 aagaaataac ttatactcct gaaaaaaaag aaatatcatt aattgccagg tattttgtaa
    33721 atggtcttgt taacggtatc catcgacata tagaattatc tcggtcaatt cgttcgcgta
    33781 tggatatagc tgaaattcct gaacaaattg ttccaatttt aaaaaaatca tcctatgcta
    33841 tttttaaact tattgaagat attgagattt tatcagataa atgtattaac gagatcaatg
    33901 aaactaattt aaagaggaga gcaacaaagg atttcccgct ttattttgct gacgattttc
    33961 ctgatgataa caggctggat tttattgtgc ttaaagatac ctatgtattg ctacgattat
    34021 ttaatagcca gataaagaag gattgggttg aattagaaaa acaaacatca gtatttaatt
    34081 tttctgaagg tggtaagcaa ttagcatttc agttgaatga tagtattgtt gaagctttac
    34141 agttatgcaa ggcaaccgat attttaattg agttaattgc caatcattta ggattgc
//
LOCUS       DAOSOX010000007        32947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1379_length_32947_cov_9.021221, whole genome shotgun sequence.
ACCESSION   DAOSOX010000007 DAOSOX010000000
VERSION     DAOSOX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1379_length_32947_cov_9.021221"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLX22_01110"
     CDS             <1..333
                     /locus_tag="PLX22_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOT18530.1"
                     /translation="PEILKKYFTQMPDNPEVYEVKDELKKLVAFSYLNLFKNSYPENV
                     DIIFCRNVMIYFDRSRQKELIANFYNILNPWGYLFIGHSETLHALSDDFQYVKIMDSP
                     VYVPKEKV"
     gene            333..812
                     /locus_tag="PLX22_01115"
     CDS             333..812
                     /locus_tag="PLX22_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOT18531.1"
                     /translation="MEFQLITVSIADYKVSHSPDILRTILGSCVGICLYDLENKVGGL
                     AHIMLPENNDKSTNPKKYADSAIALMIDEMVKKGANPELIVAKIVGGASMFKMPENSF
                     IGSIGINNVLKVKQVLDKYHINIIAEDVLGDYGRTVDFFLESGKLKIKSLGREDKEM"
     gene            825..1691
                     /locus_tag="PLX22_01120"
     CDS             825..1691
                     /locus_tag="PLX22_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT18532.1"
                     /translation="MESMTGYAFIEQSTDQFTFSIEVKTLNSKYLEVYTNIPKILKYE
                     DDTINAILKKSFERGKVEFNIDIYDWVESRPVHINKQILLKYYHEVQKALSSLKIAAS
                     FSVDALLQLDGVVHKERSILSKKSKDDIYNALQKSINATIKMRKKEGKSVKKDLESSL
                     KIIEHACIDIRKQAHAVAEYQFSRLKKSIESLLHSKIDENRLYTEIAILADKQDINEE
                     LSRMNDHIVKFKEIMAGEGQLGRQLDFLAQEMFREVNTIGSKSNNTVIAHKVVEVKNH
                     IDKIREQCRNIV"
     gene            1705..1959
                     /locus_tag="PLX22_01125"
     CDS             1705..1959
                     /locus_tag="PLX22_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOT18533.1"
                     /translation="MKSTTLINIGFGNAVVAERVIAVITPVSASGKRLREEAKENSML
                     IDATHGRKTRAIIIMDSNHVILSAMQPETIANRLNQSEEG"
     gene            1959..2576
                     /gene="gmk"
                     /locus_tag="PLX22_01130"
     CDS             1959..2576
                     /gene="gmk"
                     /locus_tag="PLX22_01130"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT18534.1"
                     /translation="MIAIIISAPSGAGKTTLIEKLIIQNPIYEFAISTTTRPIRSNEK
                     EGKDYYYVSYDEFMKMVDNNEFVEWAKVHDNYYGTTKKEIDRINANSKIPIFDVDVQG
                     ARALRNKLQKVVSIFIIPPSIKELEKRLRHRSTETQQQIKIRLTNAVSELREYINYDY
                     IVVNDDLQKALEDLGAILKAIILRREFKEKAVKNLLEGVHDYTIR"
     gene            2557..2760
                     /locus_tag="PLX22_01135"
     CDS             2557..2760
                     /locus_tag="PLX22_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18535.1"
                     /translation="MIIPLDKIIALEDNKYIFSCAAMKVVDKLGNIEGYPETDKNWKV
                     VPHILHLMLNGIIKYEFKEQLSD"
     gene            2789..3703
                     /gene="miaA"
                     /locus_tag="PLX22_01140"
     CDS             2789..3703
                     /gene="miaA"
                     /locus_tag="PLX22_01140"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT18536.1"
                     /translation="MKKVNKKKAVILVGPTASGKSDISLSIANNLFEIVSVDSVQVYK
                     YMDVGTGKVSPYHRSLITHYCIDIVNPDYWFTAGDFCREASGAVETILQHGKIPLFVG
                     GTGLYIHTFFYGLAKLPRIDSKIKEQLHAELQLKGIQALYKELSHHDKAWADKIHPND
                     TQRILRGLEVFRATGLPLSQFLQSDHMKSDLEILLIAVDIERDILYKRIECRVDTMIN
                     NGLVEEVEKLRAMGYTQDLNSQKAIGYIQLHRVLNNEISLSDAIEDIKLQTKHYAKRQ
                     ITWFKRYKPLWMPANDGKGIKKIINEWI"
     gene            3817..4047
                     /gene="hfq"
                     /locus_tag="PLX22_01145"
     CDS             3817..4047
                     /gene="hfq"
                     /locus_tag="PLX22_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOT18537.1"
                     /translation="MGKQTKNLQDTFLNAARKDKVEITIYLMNGVPIKGKVISFDSFT
                     ILVEVDKKQNLIYKHAVSTIMPSKPVQYKDEE"
     gene            4051..4935
                     /locus_tag="PLX22_01150"
     CDS             4051..4935
                     /locus_tag="PLX22_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18538.1"
                     /translation="MSKYKKLVIAAFIIAAAVYIFVKAVVFVGDDVVAIVTDKEDQII
                     GLLKKGANVVPQALFHPVRVNYIPLHGSCKADIIVPIPPLEQIKSKNYSITIPVSIQY
                     TFDADKLQLDLKSMHSNAEYVKEIITIALVDAVNTQFRKYLVPVYKYFPLQANFQNEF
                     TVVINTVKEVCVKRGLVIDDVKVGNVYLPDYAIYAEGKRFLLEVLQQEKDNALQLARL
                     QQKLNEDGLASKQRIEHLEKISELIQKNKDILRYIYIDKLADNVKVIVTSDKEAGFDI
                     FNTEDSGSVKKDVDNFKK"
     gene            5281..6009
                     /locus_tag="PLX22_01155"
     CDS             5281..6009
                     /locus_tag="PLX22_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18539.1"
                     /translation="MRRRISSKPIILPEIKKTYWKRRPSEIRGYQRVFYCNMENGDYI
                     RVDYSFKDKRVRIYLEDSEEGGNPYYAVIDKGRITAERNATSGRPVPLQEKLEKRSEI
                     LSTLPNKEVLRLINKHYGIGETPEEVAARKKKRQEELARTKKRYFKPEEVPSFVKKET
                     ALPTKRYVRIRDIVDIAVGGTIVGVVYYVSMDFVKMGIASAVFGIIMAFVDIFVRGKE
                     TLLLKTLLFLLTGIMLYIYGYFVL"
     gene            complement(6017..6976)
                     /locus_tag="PLX22_01160"
     CDS             complement(6017..6976)
                     /locus_tag="PLX22_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT18540.1"
                     /translation="MLQSILYNYDQRIHLEELLTFSMALLLNILIIIFSHFTLAPVTF
                     TINIAIMLLIIATVFAQRFATSKLFSIYRDWYVLPLLVAIFMEHNMLVPLINPNDIDT
                     YLIKIDYSLFFGNHPTLLLERVMHPFVTELLQIVYASFYFLPFTLCLLLYLKERKVEF
                     HIVASSIMFGFYLSYIGYYIFPAIGPRFTLAQLQTKPLEGLFLFNFVHHGLAYLEGVT
                     RDCFPSGHTLVSILTVLLAMRYHKRFSIIATVWTVLLIISTVYLRYHYVIDVIAGFFV
                     GLIAFRYGQMLARLYIFGNTETKTPLLESIRNLWYWLLDIRRR"
     gene            complement(7263..8246)
                     /locus_tag="PLX22_01165"
     CDS             complement(7263..8246)
                     /locus_tag="PLX22_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18541.1"
                     /translation="MQLKKEIKKITIKDPFQQFKETERVNEMRYCPLTGRVSRVLGLS
                     LKEFASNDNTHIIEESKKTCPFCPQCIDALTPKFTPPLQEQYSWGNARCFPNAFPYDE
                     NNAVVTITDQHYVALDKFTHDMLSDAFHCATNFLSDIDPVQYPYQSINCNYMPAAGSS
                     LVHPHFQIIASSTPSSYYTDILSNITIKPNLFHDYINTEKEQNERIIGLEGDSSWLVA
                     FAPMGQFDVIGILHNHIPLKDFYRYCNTFITYILKILQYMASKKIDNFNMALYGVTNN
                     ADFLPHIRICPRTTFPPYATSEINYLQMLHNDSFTLLKPEIIAAEINAYTP"
     gene            8691..10685
                     /gene="ligA"
                     /locus_tag="PLX22_01170"
     CDS             8691..10685
                     /gene="ligA"
                     /locus_tag="PLX22_01170"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT18542.1"
                     /translation="MEDIKKRYDELIKLINKYNYYYYTLDKPLVDDATYDELMNELLT
                     IEKNYPEITRDDSPSKKVGATIQTSFEQVRHDPPMLSLANAMDEGDINDFYERCCKLL
                     GTSTIEYCAELKYDGLAVELVYSNGIYVQGSTRGDGEVGEDVTENIATIKRVPTKLPG
                     DAPDYLSVRGEVIMRHGEFERLNEIRRQNGEQEFANPRNAAAGSLRQLDPNITAQREL
                     DIVLYGIGKMEPASMINTQSQLYDYFKKVGLPAPHYYEVGLLQGIKQFYKYWMENRYT
                     LDFDIDGVVVKVNDITVREKMGSTSKAPRWAIAWKFPAREAITVLKSVDYQVGRTGLI
                     TPVANLEPINIGGVLVKRATLHNFQEVKRLDIKIGDTVKVIRAGDVIPKVVEVIKDKR
                     KDVRPIAVPETCPACNSTLQHEDIYIRCVNASCPAKEYETLCFFVSKDGMDIEYVGPE
                     LLKRLYDQKKIKTIADIYALKREDLLQVERMGEKIADKIIDSINKRREVTLSHFLRAL
                     GIRNVGDHMAKVIAKHAGSLQKLMVMSQEELMTIPEVGPGVAQSVYEFFHNEGSLTLI
                     RQMFANGVTVKDEKVQEASDNPFKGKTVVFTGTMQNMTREEAEGLIESMGGRASGSVS
                     SKTDFVVAGEEAGSKLDKAKKLQIRILSEDEFMTMIGKHQ"
     gene            10682..12280
                     /gene="lnt"
                     /locus_tag="PLX22_01175"
     CDS             10682..12280
                     /gene="lnt"
                     /locus_tag="PLX22_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOT18543.1"
                     /translation="MNIVKRLAIFLYDNYYLLSVLLMYCSFTTSLNVHLPLLSVFAWV
                     SLVPLFCFVGNKPLKQVYILSFVTGILGFGAVYYWIGDFGQALPFGKPIIVALVIPCI
                     SVFFATKIFVAEYLSRKSNVPKMLIYPSVWIAIDWVQTVGTMAFPWNFWGYTQYPFTN
                     FIQIASIVGIYGITFIIIAANSSISEYITARIMNNNYRQPLWACAIICVIVIFATAYG
                     WVRLHNHPPSKNSTLRVAMIQSCFSPWENWIENRYRYLHILKQLTQQAMQHNPNLIVW
                     SESATLENISYGYYNGNLNRFGEEVLSLAAGYRVALLTGEIGVVEDFVNRRYYPQNSM
                     VLINSNGEVVDTYAKMYLVPFGEYFPYGELFPFVNTIIDAFGGSNFMPGEKMVVLSVN
                     GLKCAPLICYENIFHTLCRKYAKAGIDFYVNITNLLWTNNPVGPMQHFYFSTFRAIEN
                     GVWFVSAGNSGFTALIDPYGRITASIPLMEKTYCIGDIDTSTNTVTVYRRAGDSLLYT
                     AFLFLGILSVMIVFRTYTEKRMVS"
     gene            12280..13959
                     /locus_tag="PLX22_01180"
     CDS             12280..13959
                     /locus_tag="PLX22_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT18544.1"
                     /translation="MRIIYCTDVHGAFERVRELLYETIADVYIISGDLVDIPFYNMDS
                     AIRYYELQLYFNTMRKKMGRDDITVEDFVDELMDMPHITEEVAELGAKYQQYTIRARR
                     VMQQKYKVLANIFSFKPQVHIFALPGNYDMDLKYTSLHDRDLHLHWYQVDEAVIAGYG
                     GADTFTAGIPQKYVVPYKAGIGIDDRKNEMYNFFKAVRPTIIATHQPAHGVLDWLAPV
                     GPTGSPALRTYCDNNPVILCLTGHIHGSWGMQEVENTLYLNPSPLGDITTVHHDVLEG
                     GFFYQIELQDYTVAHVLYRKIYNERVYDLAEYAKKNGKWVETVIDDIRFSAKKKLLNV
                     DMNVQQYSHIPEIELFKEIKNFFRMFQTEESELRMEKLEEIVKRVEPAIEDIAMDVLG
                     SVNVGLSQPSSDIDMVLYLRCDFECPDHLLTCDCCKNAALLIGNALQEEYKFEVLDCI
                     NLNEVEKDIKDKNYESETLQRFVAYRSICRPINYRVIAPLEDMLNADIEFRKEVEGSI
                     RSYFRIFVTTSQHVRSFKKYESRLKSVGIRLPDSIRRKILQYLQSDEDKEI"
     gene            complement(14153..14689)
                     /locus_tag="PLX22_01185"
     CDS             complement(14153..14689)
                     /locus_tag="PLX22_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18545.1"
                     /translation="MATAPSSKLPPQLKGEIINLIEERLKELYVTCSDFRALQRVMKE
                     LAEAEKRTEQRIDSLTQRMEELAEAQKRTEQHMDVLTQALTQTQLELKQLVEEHKVTR
                     KLLGDLTNTVGYGLEDTIMKYIHDYALKEYGIAITTVDRRYVTYPDGRYDEINIYAKG
                     TKNGTNVYLIGESKAQLG"
     gene            15011..19426
                     /locus_tag="PLX22_01190"
     CDS             15011..19426
                     /locus_tag="PLX22_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT18546.1"
                     /translation="MSDSHKKQKTSKHDGTSNRKKKQISSTVSGRDLQQLDFYNIIAQ
                     KSADIFTIFDMDFNIIFVSPSIEKILGFTPQEHKKRKLDQILTPDSLQSAFNVYAEEM
                     EKEKAGNVDPDRNRIMELEHYCKDGSTRWLEVNLSMIRDAQLKPLGILAVSRDVSELK
                     DITNQLKESEKQYRILVETAKEGIWRIDSNNVTTYVNAAMADMLGYAQKEMIGRPIFD
                     FMFEDDIPVLKERIETRRVIGIADVYEDRRKRKDGSELWCIVSGQPIMDEKGKYIGSF
                     AMYTDITDRKKAEEKIAEYSTMMENVIEATQTGTWQWNLQTGALIINEMWAHLLGYTL
                     EELSPISIKTWEMLTHPDDVKKAYEDLVNHYTGEKPYYECEIRMKHKQGHWVWILTKG
                     EVIEWTPDRQPLMIYGTHTDITHFKETEQALKESRQLLNNVINTIPVRVFWKDRNLRF
                     LGCNKHFAVDAGFEKTEDIIGKTDYDMGWRSEAELYRMDDKEVIETGIPKIGYEESQT
                     APDGSNLWIRTSKIPLRLGDEIIGVLGTYEDITAKKLADEKLKESEERYRSITETTHD
                     IIVLHDMNGIIQYVNQTGINIGGYGYDEIVGKNILKFIPKKYHQSIVTLHQERINGYM
                     GLHQYETEIINKLKKKIPVEVASSPIVKNGKTVAILIVAHDLTQRKMAEELLVKNKER
                     LESIIAVLQKEAKTEEELFYHVLNEAIKLTDSTIGYAMEYNEEEKRCFIKAWSDVVMN
                     QCAIQDKPVIYNVEGAGIWVETIKQARPVVINNYNDYHPAKRGYPEGHVDIQRFLGIP
                     VFKDNRIVATVGVANKETDYTEIDILQITMLMDSAWQKIEKIKSEQALAESEEKFRSL
                     ANSTPTAILLYQDNKWIYANPAAVNIAGYSAEEFLNMNFWDIVHPDDKQIVRERGTKR
                     QTNVDTVDSYEFRIIAKDGRIKWVYLSGVSVIYKGRPAGMISILDITDRKMAEESLIE
                     EKEKLRITLQSIGDGVIATDTNGRIAVINETAQQLTGYSQEEAQGKPLSEIFVIVHEL
                     SSKPLDNPVEKVLQTQQIYELSNHTMLIAKDGTRRIIADSAAPIKDAMGRILGVVLVF
                     RDMSEKIRLIEAVQRTQRLESLGQLAAGLAHDFNNILEGVMGYIGLASTYIKDNNISE
                     LLGEALKSVQRAKGLTGQLLTFAKGGTPIKKTQSVIPLLKDTVKFALSGSNVKAEFNC
                     DEDLWNAEFDYNQIAQVIENIVINAVHAMPMGGTITVGAHNITFALNEHPLLPAGKYI
                     KIAIADKGIGIPKDILSKIFDPFFTTKSKGHGLGLATGYSIIARHNGTIEVDSIPGQG
                     TTFYIYLPATQQKVEMEEKNKTYEKKLTGGKILVLDDDAMMQKLMISFLEHLGFEAMA
                     VEDGQSAVNLFLHEKEKGTPFNALIFDLTVKGGMGGTEAIQLIRQHDTGVPAFVMSGY
                     HDDQVLANPEEYGFNNGLSKPFTIEELQHLLAKYF"
     gene            complement(19819..20313)
                     /locus_tag="PLX22_01195"
     CDS             complement(19819..20313)
                     /locus_tag="PLX22_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18547.1"
                     /translation="MKKSITKTMLIINFFYISILLLYTQTFADICVAPYQSPSGFSVD
                     LPAWDRSEMMKQDALEFICAHSQYIYIKIIKLKNPPDDILHYMSWKIWDTDPNAHFVI
                     KDKLEDGGIIISTYRNKTYSLHRVRMIQQKEYTYIIECSAPEKSFYNYEKYFNRVFKS
                     FTLQ"
     gene            20863..>21252
                     /locus_tag="PLX22_01200"
     CDS             20863..>21252
                     /locus_tag="PLX22_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18548.1"
                     /translation="MNNNSQVVSCQPIVHKIEELSCTQFTIGMLISSNYYVFTDTKVG
                     ILADELSRNDKIYAVAVVDKNKKSYGVIIRKDLFDILGTRFGRELNYNKTADEVMQKV
                     KQFNYNMHIFECAKAIEKDLLTGVQDHY"
     assembly_gap    21253..21352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21353..22476)
                     /locus_tag="PLX22_01205"
     CDS             complement(<21353..22476)
                     /locus_tag="PLX22_01205"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT18549.1"
                     /translation="MNIYFLNSSHQEIEKINTLTSLALSTQIEFEKQFSMLHYILLEK
                     DPLQYQTYFHKFSYQYTIVQNQLFNLKLLLKDFPNIHNKMNDIQKYHKDLTELLIDEL
                     FQYKDKSATVDDVYSIIKGKDIIAIESLNTIISEINNINYEIAMKTKKKYFILSIVSI
                     SLISIVTLSLVIAILYITKLEKKFIFNIGKQLSSYLPIQLVQLILRQEKQEVVPIQKK
                     FITVCFTDIEGFTKLTDAIEPEIVAEILNDYLSEMTVVAHKYNGTVDKFLGDGIMILF
                     GAPNSMPASIQIENAMTMAVEMQTIFKMLNDKWKIKINNNILHLRIGIHCGMTTVGSF
                     GPPERLSFTAIGKIVNIAFRLQQLCNSDGILISEDVKNIMP"
     assembly_gap    22513..22612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22613..24193)
                     /locus_tag="PLX22_01210"
     CDS             complement(<22613..24193)
                     /locus_tag="PLX22_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOT18550.1"
                     /translation="MTSIQQHNKHPLHYVWWKHGVIYQIYPRSFYDANDDGIGDLPGI
                     IKKFDYLIDLGIDAIWLSPINTSPMYDCGYDVSDYYSIDPIFGTLKDFKELLAIAHSN
                     NIHIIMDMVLNHTSQLHPWFVESSSSRYNMKNDWYIWHDGFNGKPPNNWKSAYLSSAW
                     EKNEMRGQYYLHSFLKEQPDLNWHNQQVKHAMFDMMQYWLDLGVDGFRFDVINYCYKD
                     KNLRNNPFSFNPFNDQIEKYNKNRLESYILVQEIRKLIDKYDNRMTVGEVFSYPPGNP
                     ALSASYLCKGKGLHLAFDFSLLYQPFDSHKIYRCIKNWYNHIPEHCWPCNVLSNHDQQ
                     RYADKFGQEDNDSIKRLMALLLLTLWGTPFIYYGEEIGMKNTSIPLRKIVDPAGKRFW
                     PMYKGRDCARTPMQWSDEANAGFSHTMCWLPIDSNYRTINVKHQLKDRYSLLNYYKDL
                     IRLRKKHHALTHGSWEPLIKGKNGVIAYIRKAASETLCILLNFNDHATHIHHHYEAQW
                     KVLFSTHRSRFEHFADLRI"
     gene            complement(24171..26825)
                     /locus_tag="PLX22_01215"
     CDS             complement(24171..26825)
                     /locus_tag="PLX22_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT18551.1"
                     /translation="MKIKNTFDEHINSLINSFVNEQKVRFPQDEILTIDLHCHDYNSN
                     VPDELLGRILGIPETWLSTDELIATLKNHGCDTFTVTNHNNARSCFELLDKGFDIVVG
                     AEFSCSVPDYGVGIHVLTYGFTSEQEIKLNKYRNDIYKFLDYTSQNDIPTIWAHPLYH
                     YKSRGIPPMDFFDKMALLFQRFEVINGQRDSWQNMLVKQWIESLTPEKIEQLSKRFGI
                     KPDRYCTQPYKKYFSGGSDSHMGIFTGLTGTYLHIPHLMNRLHHEKRSSLALEAIKSG
                     TMAPFGTHNDSEKMAISFIDYFCQIGMKMKDPGLAHILLHKGETIDKLAALTIVNSFQ
                     ELKRHKTTLHFLELFHQSLTGKTFSKSKRFYVNKDYKKVFDIAYTLSRYTKTRKKEDI
                     IFYNDSIYAIYNELAQLLMKRAHKYIHKFLNDTGSATNEFESYFQNIEIPTHFRKYIA
                     PSKGADSNGIPELNLPKIFDELSFPFLGSAVIASAFFTSARVMYKARPLLYEFAQIHN
                     TLKHPERTLWLTDTFNDPNGVANVLQLMHKEIKRNDLPIDIITVSDTLQSDEHLIIIK
                     PLKEYTIPFYQQQPLRIPDILQIHKIFKEGEYNKIIVSTEGPMGLAALWLKYAYSVPA
                     YFYLHTDWMMFAEKTLKLKNDNLDNFRRILRTFYKAFDGIFVLNNEHKKWLTGKDMGF
                     NSSKVFLTSHWVEEIFKPHKTNKEEVFGVSSTTPVILFSGRLSEEKGVMDIPEIMNLV
                     KQKHPETKIAFAGIGPKETQLKTLLPDAIFLGWVDHTLLPEVYSAADILVLPSRFDTF
                     GCVVLEALSCGLPVIAYNNKGPKDIINNGLNGYLVKNETEMAQKIIELISNPLLLNLF
                     RNQSLLRAYEFKPDTIMKQFVNDLNSAA"
     gene            27214..27426
                     /locus_tag="PLX22_01220"
     CDS             27214..27426
                     /locus_tag="PLX22_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOT18552.1"
                     /translation="MRPKIIIILIILLLFLIIVVQNVQPVIFSLFHWEVTLPLIVMLF
                     VTFVCGIIIGMAISSISWHKKKAKNN"
     gene            27437..27673
                     /locus_tag="PLX22_01225"
     CDS             27437..27673
                     /locus_tag="PLX22_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18553.1"
                     /translation="MDTGVILTIVALLSFIVLFYIFMKQLMKMVVLLLTMFIIFAGYL
                     YVKNGSLPKSLQGYLDEGKIGIEQLQHTYKKLTE"
     gene            27932..28396
                     /locus_tag="PLX22_01230"
     CDS             27932..28396
                     /locus_tag="PLX22_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT18554.1"
                     /translation="MKTQTKESVAPSMQIVCFNVGKEEYGIEILKVQEILKLPEVTPL
                     PKSAEYILGVIDLRGKVIPIIDLGVRFGISEKSAQNKRAIVVDIKGKRIGLAIDQVSH
                     VVKLEQKDIEPPPPIVKGISGRFIIGIGKVENKFVIILDIDRIFSADELTNL"
     gene            complement(28391..29569)
                     /gene="larC"
                     /locus_tag="PLX22_01235"
     CDS             complement(28391..29569)
                     /gene="larC"
                     /locus_tag="PLX22_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOT18555.1"
                     /translation="MKTLYCDIQLGASGDMLIGALLDACLPVDVLQQQLASLPFTGFA
                     IAPVKQKRYGITGTQAGITAVEDTHHRDFTAIKKIIATTSCSDTVKNNATAIFETLAT
                     AESEVHGIAKDEVHFHEVGAVDSIIDILAFCIAIEYLNVDAIYYNNFAFGSGTITTQH
                     GVIPLPAPATVRLTHNKQVTFTLNNGELTTPTAAAILVTLGTQQPSFSGCIAATGIGF
                     GTRDYGFPGYTRIILLNEIHEEESSYKHELLNVIECNIDDMNPELVPFVADALLEAGA
                     LDVYYHQVIMKKGRPGVVLWALCAIEKTDIVQKIIFSQTTTLGVRAYSVNRSSLSRQV
                     QTVTVYGEKITIKLTSRYHELSPKPEFEDCKRLALKTGKTVRTIMEEALKEYYKHQTL
                     "
     gene            complement(29566..30327)
                     /gene="larB"
                     /locus_tag="PLX22_01240"
     CDS             complement(29566..30327)
                     /gene="larB"
                     /locus_tag="PLX22_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOT18556.1"
                     /translation="MNYRPIKKLLQQFKDDTITMDDFIRELEHLNVEDLEHTTLDHQR
                     KARLGFPEVIFCQNKTPQQVADIAGAIAKQNYSFLATRASLAHFKAVKRYIKKAQYNT
                     LGKTISFSAQTMHHKPHRLLIITAGTSDIPVAEEAKETALVMGLTVETIYDIGVAGIH
                     RVFHNWDKIHEATIIIVIAGMEGALPSVIGGLAPCPVIAVPTSVGYGASFQGIAALLG
                     MLNSCAPGISVVNIDNGFGAVYCAYRIIKNLPDTQ"
     gene            complement(30331..31557)
                     /locus_tag="PLX22_01245"
     CDS             complement(30331..31557)
                     /locus_tag="PLX22_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT18557.1"
                     /translation="MAGIIDKKAESLFRFFMSKTYGETIEGLWPDLYIPTAIYAQPDG
                     ISLLPSIVQLFGSRVLFITSTVDFNQYNHLFMSISRSLQSNNIPFIVYDEFPESNTDY
                     IDSAVYFARKTHCNLIIGLGGIDSINAAKTVALLTPNYLFAHELFSYPHLYEPIPFIS
                     IPTHPLFGFEITPCTIISDIHDGIKKAFFHNYIFPKAIIIDPELSVSVEEDTIAKYTL
                     GTLAIATESVISKNINNITNTLSLRTIDLVFKNLHVALQESDNETAKLNLLMASLMAG
                     IAFSSAQLSVSLAISLALASLTQIDIASYMSVMLPHIMEYNLTSAPGKYVQMSKVMDE
                     DVRDITVIEAAIKAVEGVRKLALDIDIPQRLSQFDVNKTIFSKVAEITLSYPFIQNAP
                     RVLNRDEVETILIAAY"
     gene            complement(31584..31877)
                     /gene="flgM"
                     /locus_tag="PLX22_01250"
     CDS             complement(31584..31877)
                     /gene="flgM"
                     /locus_tag="PLX22_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOT18558.1"
                     /translation="MVIDKIGNIKNIVEPKNSKSVGKTISTNKSDSVEISSEAKKAAE
                     QSKIAHVVKQTPDIRAERVREIKEMIANGTYKFDDDRIIEKVAEKIAMQLLHK"
     gene            complement(32076..32762)
                     /gene="rsmI"
                     /locus_tag="PLX22_01255"
     CDS             complement(32076..32762)
                     /gene="rsmI"
                     /locus_tag="PLX22_01255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT18559.1"
                     /translation="MTGKLYIIATPIGNLEDITLRALRILREDTDIVYCEDTRQTSKL
                     LQHYGISIPAQSLHAHSGKTKLEYIISQLLSGKNIAYCTDSGTPAISDPGGELVALAR
                     HNNIPVIPIPGASAVTALVSVCGFPSSSFYFAGFLSKKQGKRKKQLQQLGQLQAIVIV
                     YESPYRIVKLLYDIAEIMPQATILIGREMTKHFEEFIYFTAAEVESIIPSLHQKGEFV
                     IALYNEASKH"
BASE COUNT        10764 a         5107 c         6534 g        10342 t
ORIGIN      
        1 cctgaaattt taaaaaaata ctttacacaa atgcctgata atcctgaagt gtatgaagtt
       61 aaggatgagt taaaaaagct tgttgcattt tcatatctta atctttttaa aaattcttat
      121 ccagaaaatg ttgatattat attctgtcga aatgttatga tttattttga tcgttctcgt
      181 caaaaagaac tgatcgctaa tttttataat attttgaatc catggggata tttatttatt
      241 ggccattcag aaaccttgca cgctctgtcc gatgatttcc agtatgtgaa gataatggat
      301 tcacctgtat atgttccaaa ggagaaagta taatggaatt tcaactgatt acagtttcga
      361 tagctgatta taaggtatct cattcaccgg atatattacg aacaattttg ggttcatgtg
      421 taggcatatg cctttatgat ctggaaaaca aagttggtgg attggcacat attatgttgc
      481 ctgaaaacaa cgataagtca acgaatccta aaaaatatgc cgatagtgct atagctctta
      541 tgattgatga aatggtaaaa aagggggcaa atcctgaatt gattgttgct aaaatagttg
      601 gtggtgcttc aatgtttaaa atgccagaaa attcatttat cggctctata ggtataaata
      661 atgtcctgaa ggttaaacaa gtattggata aatatcatat caatattatt gccgaggatg
      721 tattgggaga ttatggtaga acggtagatt tttttcttga aagtggtaaa ttgaaaatta
      781 agtcattagg acgtgaagat aaagagatgt agcgagatta tagtatggaa agtatgacag
      841 ggtatgcatt tatagaacaa agtacggatc agtttacatt ctcaattgaa gtaaaaacat
      901 taaattctaa atatctggag gtttatacaa atatacccaa gatattaaaa tatgaggatg
      961 ataccatcaa tgctatttta aaaaaaagtt ttgaacgtgg aaaagttgaa tttaacattg
     1021 acatttacga ttgggtagaa tcaagacctg tccatattaa taaacagata ttgttgaaat
     1081 attatcatga agtacaaaaa gcattatcaa gtttaaaaat tgcagcaagt ttttctgttg
     1141 atgctttatt gcaacttgat ggtgttgttc ataaagagcg ttcgattctt tcaaaaaaat
     1201 caaaggatga catatataac gcattacaaa aatcaattaa tgccactatt aaaatgcgca
     1261 aaaaagaagg gaaatctgtt aaaaaagatc ttgaatcttc tttaaaaata attgaacatg
     1321 catgtataga tattcgtaaa caagcccatg ctgttgctga gtatcaattt agcagattaa
     1381 aaaaatcaat agaatcatta ttacatagta aaatagatga aaacagattg tatacagaaa
     1441 ttgcgatttt agcagataag caagatatta atgaagaact gtcacgtatg aatgatcata
     1501 tagtaaagtt taaagaaata atggctggtg aaggacagct tggcagacaa ttagacttcc
     1561 tggcacagga gatgtttaga gaggttaata caataggttc taaatcaaat aatactgtta
     1621 ttgcccataa agttgttgaa gtgaaaaatc atatagataa aatacgggaa caatgcagga
     1681 acatagttta aaaggaattg aataatgaaa tcaacaacat taattaatat aggatttggt
     1741 aatgctgttg ttgctgaacg tgttattgct gttattactc ctgtatcagc ttcaggaaaa
     1801 cgattgcgtg aagaggcaaa agaaaacagt atgcttattg atgcaacaca tggcaggaaa
     1861 acgcgtgcta taattataat ggatagtaat catgtgatac tgtcagccat gcaacctgaa
     1921 acaatagcaa atagattaaa tcaaagcgaa gaaggatgat gatagcaata attatttcag
     1981 caccttctgg tgcagggaaa acaacactta ttgagaaact tattatacag aatccaattt
     2041 atgagtttgc tatttcaact acaacacggc ccatacggtc aaatgaaaaa gaggggaaag
     2101 attattacta tgtaagttat gatgaattta tgaaaatggt ggataataac gagtttgtgg
     2161 agtgggcaaa ggttcatgat aattattatg gtacaaccaa aaaagagatt gacagaataa
     2221 atgctaattc aaaaatacct atttttgatg tagacgttca gggggcacgt gctttacgta
     2281 ataagttaca aaaggttgtt tcaatattta ttattccacc gtcaattaaa gaacttgaaa
     2341 aacgtttgcg tcaccgatca acggaaacac agcaacaaat taaaataaga ttgacaaatg
     2401 ctgtgagtga attgcgtgaa tatatcaatt atgattatat tgttgtaaat gatgatttac
     2461 agaaagcctt agaagatctt ggtgctattt taaaggcaat cattctcagg cgggaattta
     2521 aggaaaaagc agttaaaaat ttgctggagg gtgtacatga ttataccatt agataaaatt
     2581 atagcgctgg aagataataa atatatcttt tcatgtgctg caatgaaagt agtggataaa
     2641 ttaggtaata ttgaaggata tcctgaaact gacaaaaact ggaaagttgt accgcatata
     2701 ttgcatctca tgctgaatgg aatcattaaa tatgaattca aggaacagtt aagcgattga
     2761 atatttgaaa taataatttt ttataagagt gaaaaaggtt aacaaaaaga aagcagttat
     2821 tcttgttggg ccaacagcat cgggtaaatc agatatatct ctttcgatag ctaacaacct
     2881 atttgagata gtttcggtag attcggttca ggtatataaa tatatggatg ttggtacagg
     2941 gaaggtttcc ccgtaccatc gatctttgat aacacattat tgtattgata ttgtcaatcc
     3001 tgattactgg tttactgctg gtgatttctg tagagaagcg tcaggggcag ttgaaacaat
     3061 attgcaacat ggtaaaattc cgttgtttgt gggtggaacg ggattatata ttcatacttt
     3121 tttttatggt ttagccaaac ttccccgcat cgatagcaaa ataaaagagc aattacatgc
     3181 tgagttgcaa cttaagggaa tacaggctct ttataaggaa ctatcgcacc atgataaagc
     3241 ctgggctgat aaaatccatc ccaatgatac acaacggatt ttacgaggtt tggaagtatt
     3301 cagagctaca ggattaccat tatcgcaatt tttgcaatct gatcatatga aaagcgatct
     3361 tgaaattctt ttaattgcag ttgatataga gagggatata ctatataagc gcattgagtg
     3421 cagagtagat accatgatta acaatggatt ggtagaagaa gtagaaaaat tgcgagcaat
     3481 gggatacact caagatttaa attcacagaa agcaataggg tatattcagt tgcacagggt
     3541 tctgaataat gagatatcat tgagcgatgc aatagaagac ataaaactgc aaacaaaaca
     3601 ctatgctaaa aggcagatta catggtttaa gagatataaa cctttatgga tgccagcaaa
     3661 tgatggtaaa ggaataaaaa aaattatcaa tgaatggata tagtggtcat taaaaaatta
     3721 ttgatattgt tttaatatat ggtatgtaat tctataagtt tgcaaaacaa tataaaaaat
     3781 ttaataaaaa tttattaaaa ataaagaggt tgcacaatgg gcaaacaaac aaaaaattta
     3841 caggacactt ttcttaatgc agcacgaaag gataaggtag aaatcactat ttatcttatg
     3901 aacggcgtac ccatcaaagg gaaggtcatt agctttgaca gctttactat attggttgag
     3961 gtcgacaaga aacaaaatct gatttataag catgctgttt caacaattat gccatcaaaa
     4021 ccggtgcaat ataaagatga agaatagcgt atgagtaagt acaaaaaatt ggtaattgca
     4081 gcatttataa ttgctgctgc tgtttatatt tttgtaaagg cggttgtttt tgttggagat
     4141 gatgttgtag caattgtaac tgataaggaa gatcagatta tcgggctttt gaaaaaaggt
     4201 gcgaatgtag tacctcaggc tttatttcat ccggtaagag taaattatat tcctcttcat
     4261 ggtagttgta aagctgatat tattgtcccc ataccgccac ttgaacagat aaaaagtaaa
     4321 aattattcaa taacaatacc cgtatcaatc cagtatactt ttgatgctga taagcttcaa
     4381 cttgatttaa aaagtatgca ttccaatgct gaatatgtaa aagagattat tactatagca
     4441 ttggttgatg ctgtcaatac tcagttcagg aaatatcttg ttcctgtata taagtatttt
     4501 ccgttgcagg caaattttca gaatgaattt actgttgtaa tcaacactgt aaaagaggta
     4561 tgtgtaaaaa gaggattggt tattgatgat gttaaagttg gcaatgttta tcttccggat
     4621 tatgcaatct atgcagaagg gaaacgtttt ttactggagg tattacaaca ggaaaaggat
     4681 aatgctctgc agcttgccag attgcagcaa aaacttaatg aagatggttt agcaagcaaa
     4741 caacgtattg agcatcttga gaaaatatca gaattgattc aaaaaaataa ggatattttg
     4801 cgctatattt atattgacaa attagctgac aatgtgaagg tgatagtgac ctcagataaa
     4861 gaagcaggat ttgatatctt caatactgaa gattctgggt ctgttaaaaa agatgtcgat
     4921 aactttaaaa aatagtatat tgattgctgg ataattttta tttttgcaaa aattgtagct
     4981 atatataagt ttttttaaaa acggcaataa ttctttttgg ttttcctgaa gttttttatc
     5041 ctgatagtga gttagatttt gtatcataat gctgcagtga tatattttta tataataagg
     5101 agagtgaaac aatatgccct gtggtaggaa gcgaaagcga agaaaaattg caaatcacaa
     5161 acgaaagaaa agaagaagac ttaatcgtca caagaagaaa ttacgataat gtaagttgga
     5221 aggagattgg catacaaatg agtcaatctc ttttctattt taatatataa aggctctgta
     5281 atgagaagaa ggatatcatc aaaaccaata atacttccag aaataaagaa aacatactgg
     5341 aaaagacgcc cttccgaaat aagaggttat caacgggttt tttactgtaa tatggaaaat
     5401 ggcgattata tacgtgttga ttatagcttc aaagataaaa gagttaggat ttatcttgag
     5461 gatagcgaag aaggtggtaa tccttactat gcagttattg ataagggacg tattactgct
     5521 gaacgcaatg ctacatcagg cagaccggta ccattgcagg aaaaattgga aaaacgttct
     5581 gaaatacttt caacactgcc aaataaagaa gtattacgtc tgatcaataa acattatggt
     5641 attggcgaaa cccctgagga ggttgctgcc cgtaaaaaga aaagacagga agaattagca
     5701 agaacaaaaa aacgatactt taaacctgaa gaagttccat cttttgtaaa gaaggaaact
     5761 gccctgccaa caaaacggta tgtacgtata cgtgatatag tcgatattgc agttggcggt
     5821 acaatagtgg gtgttgtgta ttatgtaagt atggattttg tgaaaatggg tatagcatca
     5881 gcagtatttg gaatcatcat ggcatttgtt gatatatttg tacgaggtaa agaaacatta
     5941 ttattaaaaa ccctattatt tttattaaca gggattatgt tatatatata tggttatttt
     6001 gttttataaa caactattac cttcttcgaa tatcgagtag ccaataccac aaattccgga
     6061 tactttccag caaaggagtt tttgtttcgg tattaccaaa gatataaaga cgtgcaagca
     6121 tttggccata tcgaaatgct atcaagccta caaaaaagcc agctattaca tcaatgacat
     6181 agtgataacg aagataaacc gttgagatta tcagcaatac tgtccagact gtggcaataa
     6241 tgctgaatcg tttatgatat ctcattgcta atagcacagt tagtattgaa actagagtat
     6301 gaccactggg gaaacaatca cgcgttacac cttctaaata tgccaatcca tgatgtacaa
     6361 aattaaacaa aaataaccct tccagaggtt ttgtttgcag ttgtgctaag gtaaaccgtg
     6421 ggcctatagc cgggaatata taatacccaa tatatgaaag gtaaaatcca aacataatgg
     6481 atgatgctac aatatgaaat tcaacctttc tttctttaag atacagtaaa aggcacagcg
     6541 tgaaaggaag aaaataaaaa cttgcataaa cgatttgtaa aagctcagtt acaaaaggat
     6601 gcatcaccct ctctaataat agagttggat gattgccaaa aaataatgaa tagtcaattt
     6661 ttatgagata ggtatcgata tcatttggat tgatgagagg aacaagcata ttgtgttcca
     6721 tgaagattgc cactaacagc ggcaatacat accagtccct gtaaatggaa aataactttg
     6781 aagttgcaaa ccgttgtgca aatactgttg ctataatgag cagcatgatt gctatattaa
     6841 ttgtaaacgt aacaggtgct aatgtaaaat gagaaaatat aataattaat atattaagca
     6901 gcaatgccat ggaaaacgtt aagagctctt ctaaatgtat cctctgatca taattataca
     6961 aaatactttg aagcatatat tgtctcatcc ctctcacact gttactcaat aatactaatc
     7021 cactgagcca tcgtatctac aaaaaatata tacaatcaaa aaaataatgg caataccgtt
     7081 tatgaaaaaa tttagctatt atataatagc taacattcag atataatcaa cttttagaat
     7141 cgtacgcatg acttcatggt agcatgcaat aatacgcatg gatagcacca atcaccgaga
     7201 ttgaataaat attgcttaat gctatataat tccatacatt tgtcaagaat tattatgtaa
     7261 tactatggtg tataagcatt tatctcggct gctataatct caggtttaag taatgtaaat
     7321 gagtcattat gtaacatctg caggtagttt atctcgcttg ttgcataggg tggaaatgta
     7381 gtgcgcgggc atatacgaat gtgtggcaaa aagtcagcat tattggtaac accatacagt
     7441 gccatgttga aattatcaat ttttttagat gccatatact gcaaaatttt tagtatatag
     7501 gtaataaaag tattgcaata cctgtaaaaa tctttcaggg ggatgtggtt gtgcagtatt
     7561 ccaataacat caaactgtcc cattggggca aaagccacta accaggaact atcgccctca
     7621 agtcctatta tccgttcatt ttgttctttt tcggtattga tgtaatcatg aaaaagattg
     7681 ggctttattg ttatgttcga tagtatatcg gtataatagc tggaaggtgt tgaggatgca
     7741 ataatctgaa aatgaggatg aaccagtgag ctaccggcag ccggcatgta gttgcagttg
     7801 attgactgat agggatactg cacagggtca atgtcggaca aaaaattggt ggcacagtga
     7861 aatgcatcag agagcatatc gtgcgtaaat ttatcaagag caacataatg ctgatcggtg
     7921 atggttacta cagcattgtt ttcatcgtat ggaaatgcat tgggaaagca acgagcattg
     7981 ccccacgaat actgctcttg aagagggggg gtaaactttg gggtcagagc atcaatacat
     8041 tgtgggcaga atggacatgt tttctttgac tcttcaatga tatgggtgtt atcgttggat
     8101 gcaaattctt taagcgatag tcccagtaca cggctaaccc ttcctgttaa cggacagtat
     8161 ctcatttcat tgacacgttc tgtttcttta aactgctgga atgggtcctt tatggtaatt
     8221 tttttgatct ctttttttag ttgcatttat tacctcacta tgtaaggttt ctacgattta
     8281 tagtatagat gaaagaaaat tgtttattta gtcaattaat attttttcag gatatgtaga
     8341 tgcaattgcc ttgtttttat gacaatgcca tacaacattg cggtttataa gggatatgta
     8401 ataataaggt ttgttacagt tttgctaaag gcacatgtat tttaaaaatc atggtgtagt
     8461 atatttataa tggtagtaat aaaaaaacat gatagtagta tcaagactat atcatagtat
     8521 cctgttgctc aagtctatta aaactagagc actttggtat tagcaggagc aggtaactat
     8581 tggtaaaagt acctgacaca cgatatattt gacgtattga tagtatttat tgtatgggaa
     8641 gaacatagtg tgtaaagtta aaatttttat tgtagcggga gtactgtatt atggaagaca
     8701 taaaaaaacg gtatgatgaa cttataaagt tgataaacaa atataattac tactactata
     8761 cgcttgataa accattggtt gatgatgcaa cctatgatga gttaatgaat gagctgctaa
     8821 cgattgaaaa aaattatcct gaaataacgc gcgatgactc tccctcaaaa aaggttggtg
     8881 ctactataca gacctcattt gagcaggtgc gtcatgatcc accaatgtta agccttgcca
     8941 atgccatgga cgaaggggac atcaatgatt tttatgaacg ctgctgtaaa ctgctgggga
     9001 catctactat tgaatattgt gccgagttga aatacgatgg gctggctgta gaactggtgt
     9061 acagcaatgg catctatgtt cagggttcaa cacgtggcga tggtgaagta ggtgaagatg
     9121 taacagaaaa tattgctacg attaaacgcg tacctacaaa gttgccggga gatgctcctg
     9181 attatttaag tgttcgcggt gaggtcatta tgcgtcatgg tgagtttgaa aggttaaacg
     9241 aaatccgaag acaaaatggt gagcaggagt ttgcaaatcc gcgaaatgcc gctgccgggt
     9301 cactgagaca gttagatccc aatattaccg cacaacgtga attggacata gtactctatg
     9361 gaattgggaa aatggaacct gcatctatga ttaatacaca gagccagctt tatgattatt
     9421 ttaaaaaggt gggactgcct gcgcctcatt attatgaggt tggtttgttg cagggtataa
     9481 aacaatttta taaatactgg atggagaaca ggtatacact ggactttgat attgatggag
     9541 ttgttgtaaa ggttaatgat ataacagtac gagagaaaat gggaagcact tctaaggcgc
     9601 cacgctgggc aatagcatgg aagtttcctg cacgagaagc aataacagtt ttaaaatcag
     9661 ttgattatca ggttggacgt acaggattga taacgccggt agcaaattta gagccaatta
     9721 atattggagg tgtactggta aaacgtgcaa ccttgcataa ttttcaggag gttaaacgcc
     9781 ttgatataaa gattggtgat acggtcaagg taatacgcgc tggcgatgtt atccccaagg
     9841 tagtagaagt tattaaggat aaacggaagg atgtgcgtcc aatagcggta ccggaaacat
     9901 gtcctgcatg taattcaaca ttacagcatg aagatattta tattcgttgt gttaatgctt
     9961 catgtcccgc aaaagagtat gagacattgt gcttttttgt ttcaaaggac ggtatggata
    10021 ttgagtatgt tgggccggag cttttaaaac gtttgtacga tcaaaaaaag attaaaacaa
    10081 tagcggatat ctatgcatta aaacgtgaag acctattgca ggtagaacga atgggtgaaa
    10141 agatagctga taagatcatt gattctatca ataaaagaag ggaagtaacg ctatcacatt
    10201 ttttgcgtgc actgggcatt cgcaatgtgg gcgatcatat ggcaaaggtt atagcaaagc
    10261 atgcaggatc gttgcaaaag cttatggtga tgtcacagga agagcttatg acaattcccg
    10321 aggttggtcc aggcgtagcg cagagtgtat atgaattttt tcataatgaa ggttcattaa
    10381 cacttatacg tcagatgttt gcaaatggtg ttaccgtaaa agatgaaaag gttcaggaag
    10441 catctgacaa cccttttaaa ggaaaaacag ttgtatttac cggtaccatg caaaatatga
    10501 cacgagagga agctgagggg ttaattgaat caatgggagg aagggcatct ggttcggtaa
    10561 gcagtaaaac cgattttgtc gttgcagggg aagaggctgg atcaaaatta gataaagcaa
    10621 aaaaattaca aatacgcatt ctttcagagg atgaatttat gacgatgata gggaagcatc
    10681 aatgaacata gtgaaacgat tggctatctt tttgtatgat aattactatt tgttgtcagt
    10741 attattaatg tattgttcgt ttactacatc attaaatgtg catctgcctt tgctttcagt
    10801 ttttgcatgg gtatcattgg tgccactgtt ttgttttgtc ggtaataaac cgctgaaaca
    10861 ggtgtatata ctttcatttg tcactggcat tctgggtttt ggagctgtgt attactggat
    10921 aggtgatttt ggacaggcgc ttccgtttgg aaaacctatc attgttgcat tggttattcc
    10981 gtgtatctca gtattttttg ctacaaagat atttgttgct gaatatctat cgcgaaaatc
    11041 aaatgtacca aagatgctca tctatccatc agtatggata gctatagatt gggtacagac
    11101 agtaggaact atggctttcc catggaattt ctggggatat acgcaatatc cattcacaaa
    11161 ctttattcag atcgcatcga tagtcggaat atacggcata acctttatta tcattgcagc
    11221 caatagtagt ataagtgaat atatcactgc aaggataatg aacaataact atcgccaacc
    11281 attatgggca tgtgcgataa tatgtgtgat agtaattttt gcaacagcct atggatgggt
    11341 acggttgcat aaccatccac cttcaaagaa ttcaacattg cgtgtagcca tgatacaatc
    11401 gtgtttctcg ccgtgggaaa actggattga gaataggtac aggtatctgc atattttaaa
    11461 acagctgact cagcaggcaa tgcagcataa tcccaatctt attgtgtggt ccgagtcggc
    11521 aacacttgaa aatatttcgt atggctatta taatggcaac cttaaccgtt ttggagagga
    11581 ggtgctatcg cttgctgcag gctacagggt agcactttta accggtgaga ttggcgttgt
    11641 tgaagatttt gttaaccgtc gttattaccc gcaaaacagc atggtgctta taaatagtaa
    11701 tggcgaagtt gtagatactt atgcaaaaat gtatctggtg ccttttggag agtattttcc
    11761 gtatggtgaa cttttcccgt ttgttaatac aataattgat gcatttggcg gttcaaattt
    11821 tatgcctggt gaaaagatgg ttgtcttatc agttaatggg ttaaaatgtg cgccattaat
    11881 atgttatgaa aatatttttc atacactgtg caggaagtat gcaaaagcgg ggattgattt
    11941 ttatgtcaat ataacaaatt tgctatggac caataatcct gtggggccca tgcagcattt
    12001 ttacttttca actttcaggg ctatagaaaa tggcgtgtgg tttgtaagtg ctggcaattc
    12061 tggttttact gcgctcattg atccatacgg gcgcattaca gcttcaatac cgttaatgga
    12121 aaaaacttat tgtattgggg atattgatac cagtaccaat actgtcacag tatacaggcg
    12181 tgctggcgat agtttgttat atactgcatt tctatttctg ggaatactat cagtcatgat
    12241 tgtctttcga acctataccg aaaaaagaat ggtgagctga tgcggataat ttattgtact
    12301 gatgttcacg gtgcttttga acgcgttcgt gaattgttat acgagacaat tgctgatgtc
    12361 tatattatat caggtgatct ggttgatatc ccattttata atatggatag cgctattcgt
    12421 tattatgagc tgcaacttta ttttaatacc atgcgaaaaa aaatggggcg cgatgatatt
    12481 acagtagaag attttgttga tgagcttatg gatatgcccc atattactga agaagttgcg
    12541 gagttaggag ctaaatatca gcagtatacc attcgtgctc gaagggtcat gcagcaaaaa
    12601 tacaaggtgc ttgccaatat ttttagtttt aaacctcagg tccatatatt tgcccttccg
    12661 ggtaattatg atatggattt aaagtataca tcacttcatg acagggattt gcatctacac
    12721 tggtatcagg tggatgaagc tgttattgcc ggttatggag gtgctgatac ctttactgcc
    12781 ggtataccac aaaaatatgt tgtaccctat aaagctggaa ttggaattga tgatcgcaaa
    12841 aatgagatgt ataatttttt caaagcggtg cgccctacta tcatcgctac tcatcagcct
    12901 gctcacggtg tgctagattg gcttgcacct gttggtccta ccggctcacc ggcattaaga
    12961 acatattgtg ataacaatcc tgtaatactt tgtttaaccg gacatattca tggctcatgg
    13021 ggtatgcagg aggttgaaaa tacgctatat cttaatccat caccgctggg tgatattaca
    13081 acagtccatc atgatgtgct tgagggaggt tttttttatc agattgaatt acaggattat
    13141 acagtagctc atgtgctcta tagaaagata tataatgaac gtgtgtatga tttagcggaa
    13201 tatgctaaaa aaaatgggaa atgggtggag actgtcattg atgatattcg tttcagtgca
    13261 aaaaagaaat tattgaatgt tgatatgaat gtgcagcaat attcacatat acctgagata
    13321 gagctgttta aagaaattaa aaattttttc aggatgttcc agactgagga atcagaactg
    13381 cgtatggaaa aattggagga aatagtaaag cgagttgaac cagccataga ggatattgca
    13441 atggatgtac tgggctcagt taatgttggt ttgtcgcagc ccagttcgga tattgatatg
    13501 gtgctctatt tgcgctgtga ttttgaatgt cctgatcatt tattgacctg tgattgttgt
    13561 aaaaatgctg cactgttaat tggaaatgca ttacaggaag aatataaatt tgaggtgctt
    13621 gattgtataa acttaaatga ggtggagaag gatatcaaag ataaaaatta tgaatcagaa
    13681 acattgcaac gttttgtagc ataccgttct atctgccgcc ctatcaatta cagggtgata
    13741 gcacctcttg aggatatgtt aaatgctgac atcgaattta gaaaagaagt tgaagggagc
    13801 attagatcat atttcaggat atttgtaaca acttcacaac atgttcgttc atttaaaaaa
    13861 tatgaaagta gattgaagag tgtgggaata cgcctccctg attcaatacg cagaaagatt
    13921 ttacagtatc ttcagagcga tgaggataag gaaatataat gttaattaat caaaactttt
    13981 tttatattta taaaactcaa agcttttatg aatgcgaatc attgggtagg tagtgctgat
    14041 atactgttca acatctggag cacagctata acaaactaaa aatggaataa tttcaccgtg
    14101 tagctggtca tgtaatcgtg aaatcatttt attaaaatta tctgcatctt ttttaaccta
    14161 attgagcttt actttcacca attaaatata catttgtgcc atttttggta cctttagcat
    14221 agatgttaat ctcgtcatag cgcccatcag ggtaggtaac atatcgtcta tcgacagtgg
    14281 taatagcaat tccatattct tttaaagcat aatcatgaat atatttcata atggtatctt
    14341 caagtccgta gcctactgta ttggtaagat cacccaaaag ttttcgtgta accttatgtt
    14401 cttcaacaag ctgttttaat tctagctgag tttgtgttaa tgcctgtgtt aatacatcca
    14461 tgtgctgttc agtacgtttt tgagcttcag cgagctcttc catacgctgt gttaatgaat
    14521 cgatgcgttg ttcagtgcgt ttttcagctt cagcgagctc tttcatgact ctttgtaaag
    14581 ctctgaaatc agaacatgtt acataaagct ctttcaaacg ttcctcgata agattaataa
    14641 tctctccttt taattgagga ggtaatttgg atgatggagc tgttgccata atattcctca
    14701 ttgcttttgc gcattatata ttactataca caaatataga tataccatga ttttgtttca
    14761 actaaaattt gtagtaaatt gtaaaatttt aacgcaacta ctatggtgat ctattatttt
    14821 agttgtttta attaacagtt acaattatta ttataagcgg ggttttaata attatatttc
    14881 tgatattttg ttgtgggcat aaatatttga caaaagttct attatttttt ttcaataatg
    14941 gaaacatgat ttttcaatgt gccatacaat agttaattat gatacatttt aatcatatgt
    15001 ggagccatat atgagtgata gccataaaaa gcaaaaaaca tcaaaacacg atggaacatc
    15061 taatagaaag aaaaaacaaa tatcatccac cgtttcagga cgtgatttac aacaattaga
    15121 tttctacaac atcatagctc aaaaaagtgc agatattttt accatttttg atatggattt
    15181 taatattatt tttgtaagcc catccattga aaaaatttta ggatttacgc cccaggagca
    15241 taaaaaacga aaattagatc agattttaac gcccgattca ctgcagtcag cattcaatgt
    15301 ttatgcagaa gagatggaaa aagaaaaggc tggcaatgta gatcccgacc gaaacagaat
    15361 tatggagtta gagcattatt gtaaagatgg atcaaccaga tggttagagg taaatttatc
    15421 aatgatacgg gatgctcagc taaagccttt aggaatattg gctgtttccc gtgatgtttc
    15481 tgagttgaaa gacattacta accaattaaa agaaagtgaa aaacaatatc gtatactggt
    15541 tgaaactgca aaagaaggga tatggaggat agatagtaat aatgtaacta cctacgtcaa
    15601 tgcagcgatg gcggatatgc tgggatatgc ccaaaaggag atgataggca ggcctatttt
    15661 tgattttatg tttgaggatg atatccctgt attgaaagaa cggattgaaa caagaagagt
    15721 aataggaatt gctgatgtat atgaggacag gaggaaacga aaagatggca gtgagctgtg
    15781 gtgtatagtt tcagggcagc caataatgga tgaaaaagga aaatatattg gctcatttgc
    15841 aatgtataca gatatcaccg acagaaaaaa agctgaagaa aaaattgcag aatacagcac
    15901 gatgatggaa aatgttatag aagctactca aactggcaca tggcaatgga atctacaaac
    15961 tggagccctt ataattaatg aaatgtgggc tcatctgctg gggtacactc ttgaggaact
    16021 atcgccaatt tctataaaaa cctgggagat gctaacacac cctgatgatg taaaaaaagc
    16081 atatgaagat ctggtaaacc attatacagg tgaaaaacca tactatgaat gtgaaatcag
    16141 gatgaagcat aaacagggac actgggtgtg gatactgacg aagggagagg tgatagagtg
    16201 gacgcctgat aggcaaccgt tgatgatata tggcacgcat accgacatta cccattttaa
    16261 agaaacagaa caggctttaa aagaatcccg gcaattattg aataatgtta ttaatacaat
    16321 tccggtacgt gtattctgga aagatagaaa tttacgattt ttgggatgta ataaacattt
    16381 tgccgttgat gcaggatttg aaaagacaga ggacatcata ggaaaaacgg actatgatat
    16441 ggggtggcgc agtgaagcag aactataccg catggatgat aaggaggtaa tagaaacggg
    16501 cataccaaaa attggatatg aggagtcaca aacagcgcct gatggtagca acttgtggat
    16561 tagaaccagt aaaataccat tgcgccttgg ggatgagatc ataggggtac tgggtaccta
    16621 tgaagatata acagcaaaaa aattggcaga tgaaaagtta aaagagagcg aggaacgcta
    16681 caggtcaata actgaaacta cacacgatat tatagttttg catgatatga atgggataat
    16741 acaatatgtt aaccagacag gtataaatat tggtggctat ggttatgacg agatagttgg
    16801 taaaaatata cttaaattta ttcctaaaaa ataccatcaa tctattgtta cattacatca
    16861 ggagcgcata aatggatata tgggacttca tcaatatgag actgaaatta ttaataaatt
    16921 aaagaaaaaa atccctgttg aggttgcatc atctccaatt gtaaagaatg gtaaaacggt
    16981 tgcaattctt atagttgcac atgatttaac acagcgtaag atggcagagg aactgcttgt
    17041 aaagaataag gagcgattgg aatcgattat tgctgtgtta cagaaagaag caaaaacaga
    17101 ggaagaacta ttttatcatg ttctcaatga agctattaaa ttgacggaca gcacaattgg
    17161 atacgctatg gaatataatg aagaagaaaa aagatgtttt attaaagcat ggtcagatgt
    17221 ggtaatgaat caatgtgcta ttcaagacaa gccggtaata tataatgtag aaggtgccgg
    17281 catatgggta gaaacaatta agcaggctcg tccggttgtt attaataatt acaacgatta
    17341 tcatccagcc aagaggggct atcctgaggg gcatgtggat atacaacgct ttttaggtat
    17401 acctgttttt aaagataaca ggattgttgc aacagttggt gttgccaata aagaaacaga
    17461 ttatactgag attgatatat tgcagattac catgttaatg gacagtgcct ggcaaaagat
    17521 tgagaagata aaatctgagc aggcacttgc agaaagtgag gaaaagttca gaagtttggc
    17581 aaattcaact cctactgcaa tacttttata tcaggataat aaatggattt atgcaaatcc
    17641 tgccgctgta aatattgcag gatatagcgc agaggaattt ttaaatatga atttttggga
    17701 tatagtgcat cctgatgata aacaaatagt gagggagcgg ggtactaaaa gacaaacaaa
    17761 tgtagatact gtggatagct atgagtttag aataatagca aaggatggca ggataaaatg
    17821 ggtgtattta agtggagtaa gtgttatcta caaggggcgt cctgcaggga tgatttctat
    17881 tcttgatatt accgatcgta aaatggctga agagtcactt atagaagaaa aggaaaagtt
    17941 aaggattact ttgcaaagca ttggtgatgg tgttatagca acagatacta atggcaggat
    18001 tgctgttata aatgaaacag cgcagcaatt aacaggttat tcacaggaag aagctcaagg
    18061 taaaccgctc tctgaaattt ttgttattgt tcatgaacta tcgagcaaac ctcttgacaa
    18121 tcccgttgaa aaggtattgc aaacacaaca gatttacgag ctgtcaaacc atacgatgct
    18181 tatagcgaaa gatggaacaa gacgcattat agccgatagt gctgcgccaa tcaaagatgc
    18241 aatgggtaga atactgggtg ttgttttagt atttagagat atgagcgaaa aaatacggct
    18301 tatagaagcc gtgcagcgta cacagcgttt agaatcactg ggacaattag ctgccggcct
    18361 tgcccatgat tttaacaata tattagaagg agtaatgggg tatattggtt tagcttctac
    18421 ctatataaaa gataacaata tctctgagtt attaggggag gcgttgaaat cagtccaaag
    18481 ggctaaagga cttaccgggc agttattaac ctttgccaaa ggtggtacac caattaaaaa
    18541 aactcaatcg gttattcctt tgttaaagga tacagtcaaa tttgctctaa gtggttcaaa
    18601 tgtaaaagca gaatttaatt gtgatgaaga tttatggaat gcagaatttg attataatca
    18661 gatagctcag gtgattgaaa atattgtcat caatgctgtg catgccatgc caatgggtgg
    18721 aacaattact gttggtgcac ataatataac atttgcactg aatgagcatc cactgctacc
    18781 tgccggcaaa tatattaaga ttgcaattgc agataagggt attggaatac ctaaagacat
    18841 actatcaaaa atatttgatc ctttttttac aacaaaaagc aaaggacatg gattgggatt
    18901 ggctacaggc tattcaataa tagcacgcca taatggcact atagaggttg attccatacc
    18961 ggggcagggc acaacttttt atatttatct acctgctaca caacaaaagg ttgaaatgga
    19021 agaaaaaaat aaaacgtatg aaaagaaatt aacgggaggg aagatacttg tccttgatga
    19081 tgatgcaatg atgcaaaaat tgatgataag ctttctggaa catttaggct ttgaagcaat
    19141 ggcagttgag gatgggcaaa gtgcagttaa tttgtttctt catgaaaaag aaaaaggaac
    19201 tccattcaat gccttaattt ttgaccttac tgtgaaaggg ggtatgggtg gaacagaggc
    19261 aatacagctt atacggcagc atgatactgg tgtgcctgct tttgttatga gtggctatca
    19321 tgatgaccag gtgttggcta accctgagga gtatggtttt aataatggat taagcaaacc
    19381 gtttactatt gaagaattac aacatctact tgcaaagtac ttttaattta tatttttttg
    19441 ctaaacactg ataatgatat tctcttgatt tttgcgatat taaaacaggt atctgttatt
    19501 gtattttaag catatatgca tgactcatgc atattttaag gtagcaatgg gttgataatg
    19561 atgtcatatg tgatagttat tgtaaataat gtgttagtat aaaagcagca caatttgcag
    19621 agaaatttga aagttccttg cgtatgcatt tataagataa tttatgatga aagtctatac
    19681 aaactaagta gtaattgtgt tgcaacatat cccgtaactg taattatgat tgatgattgc
    19741 caatgaattt acaatgaaat agcatgtaca ggatgtaaac tgtgtgatga acatcagggg
    19801 aattgatcat tactgttatt attgcagggt aaagctttta aacaccctat taaaatattt
    19861 ttcatagttg taaaagcttt tttcaggagc actgcattca atgatgtagg tatactcctt
    19921 ttgctgtatc attcgcaccc tgtgaagaga atatgttttg ttgcgatatg ttgaaatgat
    19981 tattccgccg tcttctaatt tatctttaat aacgaagtgt gcgttgggat cggtatccca
    20041 tatcttccag gacatatagt gcagaatatc atccggaggg ttttttagct ttattatttt
    20101 aatatagata tattgagaat gagcgcatat aaattccagc gcatcctgtt tcatcatttc
    20161 ggatctgtcc catgcgggca aatcaacaga aaaaccagaa gggctttgat atggtgctac
    20221 gcagatatca gcaaatgtct gagtatatag taaaagtatt gaaatataaa aaaaattaat
    20281 aatcaacatt gtttttgtta tagatttttt catataaatt catatcttca aaattgtgtt
    20341 atattaaaag cacccacgat aatactcaat taaaaaataa atcatagatt agatcaatta
    20401 ttttttgcaa catattttta taaaaaatat tttttaaaaa acaagtattc agtattgtat
    20461 acatctggat actacatcaa ataaagatta gctttaccac tcctgattaa aaagtagtga
    20521 tatattcata aaatatttat aagcaattaa ctgttatttt atatacaatg atttgtttat
    20581 tatatgaaga atcattaaag taatagctga aatgacaatg tacagggttt tatcctgagc
    20641 gggaaatgtc atgctgagcg atagcgaaga atctcgccgt tttgtgaacg aatagattct
    20701 tcgggactgc gtccctcaga atgacatgga aacaatgccc cctcgcctta ccatggggag
    20761 gggcagggtt gcggtgaata atgctattga tagaggtgtc catacttatt agtattcatt
    20821 ataatgataa tgataatata atttccagga ggatattagt tcatgaataa taatagtcaa
    20881 gttgtttcat gtcaaccaat tgttcataag atcgaagaat taagttgtac acagtttact
    20941 ataggtatgc tgataagttc aaattattat gtttttactg atacaaaggt tggaatatta
    21001 gcagatgagt tatcaagaaa tgataaaatt tatgctgttg ctgtagttga taaaaataaa
    21061 aaatcgtatg gagtaataat acgtaaagat ctttttgata ttttaggaac ccgttttggc
    21121 agagaattaa attataataa aactgctgat gaagtaatgc aaaaggtaaa acagtttaat
    21181 tataatatgc atatttttga gtgcgcaaaa gctattgaaa aagatttatt aacaggtgtt
    21241 caggatcatt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcattat
    21361 attttttaca tcttcactaa tgagtatacc atctgaattg cataactgct gcaagcgaaa
    21421 tgcgatatta actattttac caattgcagt aaatgataac cgttctggag gtccaaaact
    21481 acctactgtt gtcatgccac aatggatgcc aattctcaga tgcagtatat tattgtttat
    21541 tttaattttc catttatcat tgagcatttt aaatattgtt tgcatttcaa ctgccattgt
    21601 catagcattt tcaatttgta ttgatgctgg catagaattg ggtgctccaa ataatatcat
    21661 aattccatcg cctaaaaatt tatccacagt cccattgtat ttatgagcaa ccacagtcat
    21721 ttcagatagg taatcattca gtatttcagc aacaatttct ggttcgattg catctgttaa
    21781 ttttgtaaat ccctctatat cagtaaaaca cacagtgata aattttttct ggatgggaac
    21841 aacctcctgt ttttcctgac gcaggattaa ttgaaccaat tgtatgggaa ggtaagatga
    21901 aagctgtttg ccaatgttaa atataaactt tttctctaac ttagtaatat acagaatagc
    21961 tataaccagc gataatgtaa caatacttat taatgatatc gaaacaatgg ataaaataaa
    22021 atattttttc tttgttttca tcgcgatttc ataattaata ttgttaatct cagagataat
    22081 agtgtttaac gattctattg caattatgtc tttgccttta attattgaat atacatcatc
    22141 aacagtagct gacttatcct tatattgaaa caactcatca attaataatt ctgttaagtc
    22201 tttatgatat ttctgtatat cattcatttt attgtgtatg tttggaaagt ctttcaaaag
    22261 aagttttaga ttaaaaagtt ggttctgaac aattgtatat tgatatgaaa atttatgaaa
    22321 atatgtctgg tattgcaatg ggtctttctc cagtaaaata tagtgcaaca tgctaaattg
    22381 cttttcaaat tctatttgtg ttgacaaagc taatgaggtt aatgtattga ttttttcaat
    22441 ctcttgatgg gagctattaa gaaaatatat attcataaaa ccgcttatcg aaatcagtag
    22501 tgccagtact atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaattcgta
    22621 aatctgcaaa atgctcaaag cggctacgat gcgtagaaaa caaaactttc cattgcgctt
    22681 catagtgatg atggatgtgc gttgcatgat cgttaaaatt taatagtata caaagcgttt
    22741 cagaagccgc ttttcgtata tatgcaataa ccccattttt cccttttatc agtggttccc
    22801 atgaaccatg ggtaagggca tgatgctttt tgcgcaacct gatcaaatct ttataataat
    22861 ttaaaagtga ataacggtct tttaattgat gttttacatt aattgtgcga tagttactat
    22921 caatggggag ccagcacata gtatgactaa agccagcatt agcttcatct gaccattgca
    22981 ttggtgtacg tgcacaatcc ctccctttat acattggcca gaatctttta ccagcgggat
    23041 ctacaatttt acgtaacggg attgaagtgt ttttcattcc tatctcttcc ccataataaa
    23101 taaatggagt cccccataag gttaacagta atagcgccat taaacgttta atcgaatcat
    23161 tgtcttcctg accaaattta tcagcgtagc gctgctgatc atggtttgac aatacattgc
    23221 atggccagca atgttctgga atatgattat accagttttt aatgcatcgg tatattttat
    23281 gtgaatcaaa aggttgatat aacaatgaaa aatcaaatgc caggtgcaaa cctttccctt
    23341 tacacaaata actggccgaa agcgcgggat tcccaggcgg atagctgaat acttcgccaa
    23401 ccgtcatacg attgtcatat ttatcaatta atttcctgat ttcttgaact aaaatgtaac
    23461 tctccagcct gtttttatta tatttttcta tttgatcatt aaacggatta aaggaaaatg
    23521 gattgttacg cagattctta tccttatagc aatagttgat aacatcaaat ctaaatccat
    23581 caacacccag atccagccaa tactgcatca tatcaaacat tgcatgtttt acctgctgat
    23641 tgtgccagtt taaatcaggt tgttctttta aaaatgaatg aagataatat tgcccgcgca
    23701 tttcattttt ttcccatgca ctggaaagat aggcagattt ccagttattg ggaggtttgc
    23761 cgttaaaacc atcatgccat atataccaat cgtttttcat gttgtatcgt gaggatgatg
    23821 attctacaaa ccatggatgc agttgtgaag tatggtttaa aaccatgtcc atgataatat
    23881 gaatattgtt gctatgagct atcgccaata attctttgaa atcttttaat gtaccaaaaa
    23941 ttggatcaat agaataataa tcacttacat cataaccgca atcatacata ggggatgtat
    24001 taatgggaga tagccatata gcatcaatgc caagatctat caaataatca aactttttaa
    24061 ttatgccagg taaatcacct atgccatcat catttgcatc ataaaaactt cggggatata
    24121 tctggtatat aacaccatgt ttccaccata cataatggag aggatgttta ttatgctgct
    24181 gaattgaggt cattgacaaa ttgtttcatt atggtatcag gtttaaattc atatgccctc
    24241 aacagcgatt gattgcggaa tagatttagc aacagaggat tgcttataag ttctattatc
    24301 ttttgggcca tttcggtttc attttttacc agataaccat tcagcccatt gttaataata
    24361 tctttgggtc ccttgttgtt gtatgcaata actggcaatc cgcatgataa tgcctccagt
    24421 acaacacagc caaaagtatc aaagcgggag ggcagtacaa gaatatctgc tgctgaatat
    24481 acttcaggta acaaggtatg atcaacccag cccaggaaga tggcatcagg caataatgtt
    24541 ttcaattggg tttcttttgg tccaattcct gcaaaagcaa tttttgtttc aggatgtttt
    24601 tgttttacta aattcattat ttccggtata tccataacgc ctttttcctc ggataatcgt
    24661 cctgaaaata gtattaccgg cgtggtgctg gatactccaa atacttcttc cttattggtt
    24721 ttgtgaggtt taaaaatttc ttcaacccag tgagatgtta aaaatacttt tgaggagtta
    24781 aaccccatat ccttaccggt taaccatttt ttatgctcgt tgttcaatac aaatataccg
    24841 tcaaatgctt tatagaatgt acgcaaaatt cttctgaaat tgtctaagtt atcatttttt
    24901 agttttaagg ttttctcggc aaacatcatc cagtcagtat gtaaataaaa atatgctgga
    24961 acagaataag catactttaa ccataatgct gccaacccca taggaccttc agttgaaaca
    25021 ataattttat tgtattcacc ttctttaaaa attttatgaa tctgcaatat atcaggtatt
    25081 cgcaacggtt gctgttgata aaatggaatg gtgtattctt taagtggttt tatgatgatt
    25141 agatgctcat ctgattgtaa tgtatcactt actgttataa tatcaattgg cagatcattg
    25201 cgctttattt ccttatgcat caactgcaga acattagcaa ctccattggg gtcattaaat
    25261 gtatcggtta accatagtgt acgttcaggg tgctttaatg tattatgtat ttgtgcaaat
    25321 tcatagagca gtggtcttgc tttgtacata accctggcac tggtaaaaaa tgctgatgca
    25381 ataaccgcag aacccagaaa ggggaatgac agctcatcaa aaatctttgg aaggtttagt
    25441 tcaggaatac cattactatc tgctcctttt gaaggagcaa tatattttct gaaatgagtt
    25501 ggtatttcaa tattttgaaa atatgattca aattcatttg tagcggaacc tgtatcgttc
    25561 aaaaatttat gtatatactt atgtgctcgt ttcatcaata gttgagcaag ctcattatat
    25621 atagcatata ttgaatcatt ataaaaaatt atgtcttctt ttttacgggt tttagtataa
    25681 cgtgataatg tatacgctat atcaaatacc tttttatagt ctttattaac ataaaaacgc
    25741 tttgattttg aaaatgtttt accggtcaat gattgatgaa aaagctctaa aaaatggagc
    25801 gttgttttgt gtcgctttaa ttcctgaaat gaattgacta ttgttaaagc cgctaattta
    25861 tcaatggttt cacctttgtg caatagaata tgggccaaac caggatcttt catcttcatt
    25921 ccaatctggc aaaaatagtc tataaatgat attgccattt tttcactatc gttatgtgtt
    25981 ccaaatggtg ccatggtacc gctttttata gcctcaagcg ctaacgatga tcgtttttca
    26041 tggtgtaatc tgttcattaa atgaggaata tgtagatatg ttccggtgag accggtaaaa
    26101 atccccatat gactatcaga accgcctgaa aaatattttt tatacggttg agtgcaatat
    26161 ctgtctggtt ttattccaaa tctttttgaa agctgttcaa ttttttctgg tgtcaatgat
    26221 tcaatccatt gctttacaag catattctgc cagctatctc tttgaccgtt aataacttca
    26281 aatcgttgga acaaaagtgc catcttgtca aagaaatcca ttggggggat gcctctggat
    26341 ttataatgat acagcggatg agcccatata gtgggtatgt cattttgtga tgtataatcc
    26401 agaaatttat agatgtcatt tcgatattta ttaagtttta tctcctgttc ggatgtaaat
    26461 ccataggtaa gaacatggat gccaacacca tagtccggaa cactacagct aaattctgca
    26521 ccaactacta tatcaaaccc tttatctaga agttcaaagc atgagcgcgc attgttatga
    26581 ttggtcaccg taaaggtatc acagccgtga tttttaagag tagcaatcag ctcatcagtt
    26641 gatagccatg tttcagggat acctaaaatt cttccaagca attcatcagg aacattgctg
    26701 ttgtaatcat gacagtgaag gtctatagtt aaaatctcgt cttgcggaaa tcttactttt
    26761 tgttcattga caaaactatt aataagtgaa ttaatgtgtt catcaaatgt attttttatt
    26821 ttcataactc ctcttcagta ttaattgcat aaatctaatt ataaaattgg ctcttcatgg
    26881 taaatatttt atgaacttat gatgagtttt ttataaatat gacctgaaac gaggacgtca
    26941 tcctgagcga tagtgaagaa tcgcaacgtt ttatgaaaga atagatgttt cgggactgcg
    27001 tccctcgtaa tgacaatgat acaataatgt tattttcagg aaaaaagctg attgtttttt
    27061 gcaggctaca ataaaatgtt tgattatatt atattctgtt gcaattatag cgataattat
    27121 acagggaact atcgtaccta ttattttttt attgtatatt ttgtactatt ggaaaatagt
    27181 atatgtcatt attataaatg ttgagagaat ataatgcgac caaaaataat cataatttta
    27241 attattttac tgctattttt gataatcgtt gtacaaaatg ttcagccagt aatattttca
    27301 ttgtttcatt gggaagtaac gcttccactt attgttatgc tgtttgtaac atttgtttgc
    27361 ggtatcataa tagggatggc aattagttca atttcatggc ataaaaaaaa ggcaaaaaat
    27421 aattaaaggt acaaccatgg acaccggtgt tatattgact atagtagcgt tgcttagttt
    27481 tatagttttg ttctatatat ttatgaaaca gttgatgaag atggttgtgc tactgctcac
    27541 catgtttatt atatttgcag ggtatcttta tgtcaaaaat ggttcattgc ctaaaagctt
    27601 acaggggtat cttgatgaag ggaagattgg tattgaacag ttgcaacaca cctataaaaa
    27661 gctaacagag taattgtaca tacatcacat atttgccttg acttaagaat catacagtat
    27721 aaaaaaaatt gcaatgaagc catgctatac cgattgatta tggtagcagc ataattttca
    27781 aagatttaac gaaacatgaa atattgcatt gtactatgag aagtcataaa aaggtaatta
    27841 aaaaaaataa taaattttta acttgaaaaa aattgtaaat aaactactat agaataaaag
    27901 gtacagttaa ccattaaaca aggaatcgtt tatgaaaacc cagacaaaag aaagtgttgc
    27961 accctccatg cagatagtgt gttttaatgt tggcaaagaa gaatacggta tagagatatt
    28021 aaaggtacag gaaattttaa aacttcctga agttacccca ttgccaaaat ctgcggaata
    28081 tatccttggt gttattgatt tacgcggcaa ggtaataccc attattgatc tgggagtacg
    28141 gtttggaatc tctgaaaaaa gtgcgcagaa caaacgtgct attgtagtgg atattaaggg
    28201 aaagcgcata gggcttgcaa tagatcaggt aagtcatgtt gtaaagttag agcaaaaaga
    28261 cattgaacca ccaccaccta ttgtcaaagg catatcaggc cgatttatca ttgggatagg
    28321 gaaggttgaa aacaagtttg tcatcatact tgatattgac aggatatttt cagcagatga
    28381 attgactaat ttataatgtt tggtgcttgt agtattcctt cagagcctct tccatgatag
    28441 tacgcaccgt tttgcctgtt ttaagtgcta accgtttaca atcttcaaat tcaggtttcg
    28501 gcgatagctc atgataccgg ctggttagtt ttatggtaat cttttctcca tatacggtca
    28561 ctgtctgcac ctgccgtgat aacgaagaac ggttgactga ataggctctg acccccaatg
    28621 ttgttgtttg cgaaaaaata attttttgta ctatatcagt tttttctatg gcacacagtg
    28681 cccagagtac aacaccggga cggccttttt tcatgataac ctgatggtag tacacatcca
    28741 gtgcacccgc ttccagaagt gcatcagcaa caaaaggcac cagttcagga ttcatatcat
    28801 cgatattaca ttctatgaca ttcaacagtt catgcttgta gctgctttct tcttcatgta
    28861 tttcattcaa tagtatgatt cgggtataac ccgggaatcc gtaatcgcgc gtgccaaatc
    28921 caatgccggt ggcagcaata cagccgctga aagaaggttg ctgggtaccc agtgtaacca
    28981 gtatggcagc agcggttggc gtggtaagct ctccattgtt caaggtaaac gtaacctgtt
    29041 tgttgtgggt aagccttacc gtagcaggag ctggtagcgg aataacacca tgctgtgttg
    29101 taatggtgcc actgccaaat gcaaaattat tataatagat tgcatcaacg tttaaatact
    29161 ctatcgcaat gcaaaatgcc agtatatcaa tgatgctgtc aactgcacca acctcgtgga
    29221 agtgaacttc gtcttttgct atgccatgga cctcgctttc agcagtagcc agtgtttcaa
    29281 agatggctgt tgcattgttc ttcacggtgt cggagcatga ggtggttgca attatttttt
    29341 ttatggcagt aaaatctcgg tgatgggtgt cttccacagc agtaatccct gcctgcgtgc
    29401 cggttatacc ataccgcttt tgttttacag gagctatcgc aaagccagta aacggtaacg
    29461 atgcaagctg ttgttgcaat acatcaacgg gaaggcatgc gtcaagcagt gcaccaataa
    29521 gcatgtcacc tgaagcacca agctgtatat cgcagtaaag tgttttcatt gggtatccgg
    29581 caggttttta ataatacggt atgcgcaata taccgcgcca aaaccgttat caatattgac
    29641 aacgctgatt cccggtgcgc atgagttgag catcccgaga agcgcagcaa ttccctgaaa
    29701 tgatgcacca taaccaaccg aggttggtac ggcaatgacg gggcagggtg ccaatccgcc
    29761 aataaccgat ggcagcgctc cctccatacc ggcaatcact ataatgatgg ttgcttcatg
    29821 gattttgtcc caattgtgaa ataccctgtg aatacctgca accccaatgt catagattgt
    29881 ttctaccgtt agtcccatca ccagtgctgt ttcctttgcc tcttctgcaa cgggaatatc
    29941 cgaggtaccg gcagtaatta tcaaaagcct gtgtggttta tgatgcatgg tttgtgcact
    30001 aaatgatatt gtttttccca atgtgttgta ctgtgctttt ttaatgtaac gttttaccgc
    30061 tttgaaatgg gccaatgacg cacgggtggc taaaaagcta tagttttgct ttgcgatagc
    30121 tccggcaatg tctgccacct gctgaggtgt tttgttctgg caaaagatta cctccgggaa
    30181 gccaaggcgt gcttttcgct ggtggtccaa tgttgtatgt tcaagatctt caacattaag
    30241 gtgctctaat tccctgatga aatcatccat ggtaatggta tcatctttaa actgctggag
    30301 gagtttttta ataggtcgat agttcataga ttagtatgca gcaatcagga ttgtttcaac
    30361 ctcatcacgg ttgagcactc gcggagcatt ttgtataaag gggtagctca gtgttatctc
    30421 tgcaaccttt gaaaagattg ttttattgac atcaaattgc gacaaccgtt gtggaatatc
    30481 aatatcaagc gctaactttc gtacaccttc aacagctttg atagccgctt caataacggt
    30541 gatgtcgcgc acatcttcat ccattacttt ggacatctgt acatatttac caggtgcgga
    30601 ggtcaggtta tattccatga tatgcggtag cataacactc atatatgatg caatatcaat
    30661 ctgtgtcaat gaagcaagcg caagtgatat tgccagcgaa acagaaagct gagcactgct
    30721 aaaagctatt cctgccataa gggaagccat caataaatta agttttgccg tctcattatc
    30781 actttcttgt aatgctacat gcaggttttt gaacacaagg tcaatagttc tgagggagag
    30841 ggtattggtg atattgttga tgttttttga tataaccgat tctgtggcaa ttgccagagt
    30901 gcccagtgta tatttagcta ttgtatcttc ctcaacactt actgataatt cgggatctat
    30961 gatgatagct tttgggaaaa tgtagttatg gaaaaatgct ttttttatac catcatgaat
    31021 atcactaata atggtgcatg gcgttatttc aaaaccaaaa agaggatgag tgggtatgct
    31081 tataaaaggt attggctcat aaaggtgagg gtatgaaaat agttcatggg caaagagata
    31141 gttaggtgtt aacagcgcca ctgttttagc agcattgata gaatcaattc cccctaaacc
    31201 aatgataaga ttacaatggg ttttacgagc aaaataaact gcagaatcaa tatagtcggt
    31261 gttactttca gggaattcat cgtatacaat aaaaggaatg ttattgcttt gtaagcttct
    31321 ggagattgac atgaagagat ggttatactg gttgaaatca accgtacttg ttataaacaa
    31381 aacacgtgat ccaaaaagct gaactataga aggaagtaac gatatgccat cgggctgtgc
    31441 atatattgcc gtaggaatat aaagatcagg ccacaatcct tcaatagttt caccatatgt
    31501 tttagacata aaaaaacgga ataatgattc cgctttttta tcaattatgc cggccatcag
    31561 ctttaacctt aaaaaggaag ttattatttg tgcaaaagct gcatagcaat tttttcagca
    31621 actttttcta tgatacgatc atcatcaaat ttataagtac cattggcaat catttcttta
    31681 atttctctaa ctcgttcggc tctgatatca ggggtttgct ttacaacatg agcaatttta
    31741 gattgttcgg cagctttctt agcttcgctg gaaatttcaa cagaatcgct tttatttgtg
    31801 gatatagttt ttcccacaga tttagaattc ttaggttcaa cgatattctt gatatttcct
    31861 attttgtcta taaccattgc aatcaccctt ttttaatcta tagtagctat cggcatatca
    31921 ttataaaaac ttaatcattt aatggtataa atataaactc tccctataaa ttaatcaagt
    31981 aattatttca taaaatggta aggtatgcga gccattcaaa aatagttaac catagctatt
    32041 tcatcactat ttatgcttta aaaggtattg caagctcaat gtttagatgc ttcattgtat
    32101 agtgctataa cgaactcgcc tttttgatgg agagagggaa ttattgattc aacctctgca
    32161 gcggtgaagt atataaattc ctcaaaatgt ttggtcatct ccctgccaat caggatggtt
    32221 gcctgcggca ttatttcggc aatatcgtac aataatttga ctatccggta aggagattca
    32281 tatacaataa ctatcgcctg caattgccct aactgttgca actgcttttt gcgcttcccc
    32341 tgttttttac ttaaaaagcc agcaaaataa aaagagcttg aggggaaacc acaaacggat
    32401 accagtgctg taacggctga tgcccccgga atggggatta ccggtatgtt gttatggcgg
    32461 gctaatgcta ccagctcacc gcccgggtca gatattgcgg gtgtgcccga gtcggtacaa
    32521 taggcaatat tttttcctga tagcaactgg cttataatgt attccaattt tgttttgcct
    32581 gaatgggcat gcaatgattg tgcggggatg ctgatgccgt aatgctgtaa aagtttgctt
    32641 gtttgacggg tatcttcgca ataaacgatg tcggtatctt ctcttaaaat acggagcgca
    32701 cgcagggtga tatcctctaa attaccgatt ggtgttgcta tgatataaag tttacctgtc
    32761 atagagtgtt ttcatttatt tacttgattt ttttggcagt ataccaacaa tagcttttct
    32821 ttcaagtata aagttaaaat tattgttaag gggagcagaa tggggcggat ttttaatttt
    32881 tcagcaggtc catcgatatt gccagttgag gtattggaag aagcttcacg ccagcttgtt
    32941 gattata
//
LOCUS       DAOSOX010000008        30348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1526_length_30348_cov_8.917638, whole genome shotgun sequence.
ACCESSION   DAOSOX010000008 DAOSOX010000000
VERSION     DAOSOX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30348
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_30348_cov_8.917638"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLX22_01260"
     CDS             complement(<1..415)
                     /locus_tag="PLX22_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT18560.1"
                     /translation="MNSFREYFVYRDDQLYCEDVSLADVARIYGTPLYVYSSKCFIDK
                     FTLLDEALASVAHTICYSVKSNSNVSILKLMKDVGCGMDVVSGGELYRAIAAGVNPEK
                     IVYAGVGKNVEEIEYALKTGIQMFNCESLSEIKRIN"
     gene            complement(412..3108)
                     /gene="greA"
                     /locus_tag="PLX22_01265"
     CDS             complement(412..3108)
                     /gene="greA"
                     /locus_tag="PLX22_01265"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT18561.1"
                     /translation="MENEKIFERVSSMFKEEMWGRLDPKDVGISRFRILDDLFNSVVD
                     AGVLEEVRNMCKEHIVSHPDSIVASYLIGLTGYHLDIIDDKIEMRKLIEMYQKYHKWV
                     IAELLSEKVLEYGESSFALKALATSLERLGRSKEAIPVWENLLKIDRFDAEVAKKLAV
                     ALIDEDKQKSIQYMKLSIEGHIKNGLYDEISDLWNKLVQVSWEDMPFFERIERMLIEA
                     RQYELTSSLLKVLLHKYKDEEHPDQSIQILKKILEYTPNDTAARRELVKLYEKKYGSH
                     SQFQLFLKLSKLTNFKYPVKHAIQDFENSIIFDKGHYVRHRSWGIGRIIDITPNTIVI
                     DFKEKQEHQMSVQMALQALTPLRSDHIQVLKCEDYEGLKKLFDEDLLEFFEILIKSYN
                     GRITVADIKKELIPDFVSEKTWAKWWSSKRSEIRKSPLFGISPEKKDMFIMRDKPLTF
                     ADELLDNFLKTDSFTDKLNVAIEFVNNIEPSEGAAVAQFFVDYFSGELKGDSVTRKIL
                     SYFILSGLSKFIDAKKLKLDQAYKMVVSIVKEYPDIPSISKKIDSYDYKKDFINLIKD
                     AREDWADIYGRVLFETPVRIHKYIVNILIRSHEYGIINKFVEQALISAKEFPEIFLWV
                     AKNLLTKSWDYDWLDYSREQLVLTLFRLMNELKKIELKGNKLKNVAIELLFGGDAAVL
                     KDVVNNFDVQFLGKIFDIAASIPYFDDSMREKFLDIIKSRFPEFNPQQNVIEEEWRID
                     AEILITTPEGFARKQEELNRLINIELATISKELAKVADATGDVRENVEYNSLLEKQSI
                     LKRDINRLENEIKKAQILDMNSISLDKVTVGTVVTISGNGEKETFAVLGPWDADFEKR
                     ILSYRSPIAQALLGKKVGDKVEIRKSEQMKQYTIETIEKYNQ"
     assembly_gap    3128..3227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3228..3937)
                     /locus_tag="PLX22_01270"
     CDS             complement(<3228..3937)
                     /locus_tag="PLX22_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOT18562.1"
                     /translation="MRYIRFSAIIVVIVGMVLVGFFPEKVQSRIVTNVEPDISAEQYS
                     ALVVEDFETQADWQLETIPKKNPDPKKDPVPVLELKYVDGGPADLLTEKWSPINKGLE
                     KKKCLGVHFKFRYPGFNSVHLLPPLEVQWDSPGKAVLTYDSRSGKDIQERAIQLPGRA
                     KAISMWVHSRGNNYTLDVWIKDYKGDVHVLKMGSLNFVGWRPMKAYIPEYVPQETNSY
                     PQTRVTKIVRFVIRANPEA"
     gene            complement(3978..4718)
                     /locus_tag="PLX22_01275"
     CDS             complement(3978..4718)
                     /locus_tag="PLX22_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOT18563.1"
                     /translation="MRREVRSFILSLAIGGLMVAVGGGNIVVQAQEAQQAQQQKKQQT
                     GLVEDWMDDFEASEDWRAMATCPIGETKIRKIPGKPRPIDENGNEVNYPDTITDENGI
                     THENKYVLGVKTYFMDRGFDRVEVFPPNEYIVRGKAKEIKVWALGRKFRHTLFVKLRD
                     YKGAIHKIKIGRLDFWGWREMSVIVPGWLPQSPGYALLDKNLHFVSFYVESDIYEVPG
                     TFYFYLDNFRVITDLSEFTGDTSIRDNW"
     gene            5022..5885
                     /gene="metF"
                     /locus_tag="PLX22_01280"
     CDS             5022..5885
                     /gene="metF"
                     /locus_tag="PLX22_01280"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOT18564.1"
                     /translation="MDIAVCLQHKKPLLSFEVFPPKTSSGEENLFKELSIMVHYKPDF
                     ISVTYGAGGSTRDKTLEIAIRIKKSFSIEPLVHFTCVGQSKDDIVSYLHYVKTLGFSN
                     ILALRGDPPMGETHFKPHPGGFTHANELVSFIRSMNHFTIGVAGYPEGHIEAPDLDTD
                     IANLKKKVDAGADFIITQLFFNNDKFYTFLDKTIKAGITIPILPGIMPLTSIAQLTKI
                     TTMCNPDIPNSLLQELEKCSQPDDMCKVGIEFTIQQCQQLRSYGVPGFHFYPLNKSAA
                     IKLILDEFWHT"
     gene            5948..6838
                     /locus_tag="PLX22_01285"
     CDS             5948..6838
                     /locus_tag="PLX22_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18565.1"
                     /translation="MKSYPFYFYTGIMLIAFMQLCILFHVSHVTIWATPTLWTGLILL
                     TDGILAKSNRSLIKNRTIVKLSLLSFLCWWMFEWFNIFLSNWHYQGLIQSLPIRYFGY
                     IWSFSTIFPGVLLVYAIIATILPKLFFKPIHITSSFLVVSFIIGLFFIFIPIIPFSMH
                     YTNRAADPDLFFWLTWAANIHYSEFTAGFTWLGFIFLLEPVNYLLNRPSIYADLIVGK
                     YTRLVSLLAAGLLCGYLWEFWNYWAATKWIYTVPILPSLKLFEMPIPGYIGFLAFAWE
                     IYAITALVYPKALYTLEGER"
     gene            6843..8471
                     /locus_tag="PLX22_01290"
     CDS             6843..8471
                     /locus_tag="PLX22_01290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOT18566.1"
                     /translation="MEDIIVIGHKNPDTDSICAAYCYAQLKNSVDSTNRYRAYRCGNI
                     NRQTRFVFNQIGLQPPDLINNVYPKVNDIMTKNVITAYADDPLYSIIKHLEQDRIRII
                     PIVDSTNVFKGIVSFNEIAQFYFPVTSIAKPVYLFKPQNFNLTIPGRFIQQGKDKEFE
                     AAIVIGAMESTRSIEELHNVIPEKTVLLVGDRKEIIEFAIKNNFAALLITGVNDFTYD
                     TTHYKGCIYLSEIDTYNTLRMLTLSIPCHSIMQDADTISPDLPVEQAREQLLLNEYRG
                     LPVCDNTILKGIITRSDIIKHYAKKVILVDHNELSQAVDGAQTAQIIEIIDHHRLGSL
                     KTTTPIFVYSKPVGSTCTLVYELFKYNNVKPEPHIALLLCSGILSDTTMLLSPTTTET
                     DKKSLEELAAIAGINYNDYGQKLLSSTESLKTIDPIYAVSNDCKIYEEYGIAVAISQV
                     EVITLDGINEVKESLLSALVQFAQSRNVHWALLLVTDITTQNSILLSTDFKGEVHLNY
                     PPIEPHIYSLPGILSRKKQLLPEILRILEELSHK"
     gene            complement(8543..9394)
                     /locus_tag="PLX22_01295"
     CDS             complement(8543..9394)
                     /locus_tag="PLX22_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT18567.1"
                     /translation="MIINHNLSAINSNKVLKFKHWDVNRSMEQLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLNQTATILQRIRVLAVQSSNGIY
                     TLEDRQLIQVEVSALVDEIDRIASQAEFNKFKLLLGDFSRTNPKASMWFHMGANMHQR
                     ERVYIGTMTSLGLNLREKTGKFLANLSTAEGANRTIGIIDEGLQKIAKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDADMAETMVDLTKDQILVQSGTAMLAQANLRPRT
                     ILQLLGT"
     gene            10076..11083
                     /locus_tag="PLX22_01300"
     CDS             10076..11083
                     /locus_tag="PLX22_01300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT18568.1"
                     /translation="MGLNPFKESNLYDLQKQIQDIVFEKWEQISSKIKAKWEHIKKRG
                     NERLTIMIIPHSEKKIVNLHITLFTLTGIITSIVLILALTSVFIINHTSTIKEVSRLK
                     MYGSNSKIQIAKYKEEVNKLYDIFQHLKPELSYLYSLTPGNRADTLWAKGGVSGIVDT
                     EEPGSPPIEILNIEEMVQDLETTKDVLKDIKKFMRERRKVIENTPSMWPVDGFVITRY
                     GIRTSPFTFQKEFHEGVDIAAFPGAPIKVTAPGIVESIVWNNQLGLTVSIKHKYGFVT
                     AYSHCQRVTVEEGQKVAKGEVIGYVGKTGNATRHICFYQIKIGTEFVDPLPYLNKVVN
                     Q"
     gene            complement(11107..12129)
                     /gene="fliM"
                     /locus_tag="PLX22_01305"
     CDS             complement(11107..12129)
                     /gene="fliM"
                     /locus_tag="PLX22_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOT18569.1"
                     /translation="MTEILSQDEIDALLTAISTGEADTTDYTAVKEQRKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVSVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAVLEIDPSITFTIIDKLFGGTGESTKISRELTDIELSVMEGIIVRI
                     LGNLREAWSNVIDLRPRLGNIETNPQFAQIVPPNDMVVLVTLETKVGEVEGMMNLCIP
                     YITIEPVISKLSAQYWYSSIRKGATDENAAIIQSRLESVELPIIVEVGEVEITMEELL
                     AIQLGDVIKLPNTKVNSDMTAKIGGRKKYKCRPGVIGNRVAVQIGEKIEDIPDELLVS
                     KRSEEE"
     gene            complement(12133..13044)
                     /locus_tag="PLX22_01310"
     CDS             complement(12133..13044)
                     /locus_tag="PLX22_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18570.1"
                     /translation="MKKLTITIATLLFAVSIYSESADYLNVTLVSPKISYDENDDIIV
                     YCVVHNTAQVPVEFTIYDIPYTTFQPVVYLQNGKEAPITVDYRLKNIPAYRLVAEEEG
                     RKIILQPDEKFVYRAVCNNFYTLAKDNKYRIKVYFYPDAKDEYTIKSDNELTIAVVPV
                     PEFAMRTGIVKKERNITPSEVVLLTLNAEMNKKWDNVIKYINIEKYIYAYSEFAKRYS
                     TTNDYERLIVEQEFINFITKDRPDYLIDFSVLHEQIIAQTNVAYVDVKVKRWGPRFPA
                     TMKYRYTLEPYNNLWVIVNVDVSILKE"
     gene            complement(13123..15000)
                     /locus_tag="PLX22_01315"
     CDS             complement(13123..15000)
                     /locus_tag="PLX22_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOT18571.1"
                     /translation="MDPTELTLKNIYSIEGRGHFPRPDCIRLPSFNLHGQWIMAFDKQ
                     NRGIRLQWYNKENAGKIISGHYILKGFESYPVEVPFPLEADVNSAIHVKSHMAPDEII
                     KQKRFWYFCSFGVDAIKSGRLHFGACDYKATVWLNGRCLGFHEGGYTPFSFFVPEFED
                     INYLVVLVEDSISPSQIRGKQTFLHKPFLVWYTNSSGIWQDVWIEQVASTHITSLTCT
                     RDAAVCFAMSVESQENVRNDITVELKIFASQVFGKRSVLKTPVKVYRDIVVFDVFKKG
                     QVVIEVPLSLFDVWSPSHPAVHPIQVVLKKAGKEIDCIHSYFGRRDVTIQNGRILLNK
                     KNIYQRLLLNQGYYPEGLYRPVDTMQYKSDIEIMKSLGFNGCRIHQKIEDPRFLYWAD
                     MVGFLIWEEMPSYYLPLQKNMQRLYAQLQEVMARDCMHPSIITLVLYNESWGLYSMFF
                     SRKVRNSVVELFDTVKSQYHGYLIIDNSGFHHLKSDITDIHHYLQRFEDIEELYKRLV
                     DGVREAPLWTHFFMMILGKENIQTPYLKGYGDTTSPIIISEMGGFGFGIYPSEAKTLE
                     ESFQKHCSILSKFPQIQGFCYTQFTDTFQETNGVVTFDRRPKAKLLPIMQKALAKHIF
                     W"
     gene            15489..17234
                     /locus_tag="PLX22_01320"
     CDS             15489..17234
                     /locus_tag="PLX22_01320"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18572.1"
                     /translation="MNYDIEQFSAINKRINSSLKLSDLLTIILDTTKDMLKSEGCSIL
                     LTDPITEDLYFYIVRGGGDSLIKGEIVPKGKGIAGYVALTGTPLIVNNPYNDERFYED
                     IDSKSNFHTRNIICSPMILIDRLVGVIEVVNSTERESFTDDDLKVLMYIADSAAIAIT
                     HRQLYHDLATRLKELEIMYEISQAIAHSSPSENIFPKIMQPLASSVGVKRASILLYNT
                     NTDEFSIAASHNLPEAINLYVNKDNAPIAYRVYTSGDPLLVSNIMSDPSLASFARTHE
                     YITPSFISIPLRYKNTIIGVLNLADKVDKSSFDAHDLRVATTIANQIVEAYQNSIYLQ
                     QTEEQRRLAQEIDIAAEIQKRILPKIPHSINSHRLAAFNKSAKEIGGDFYDIFQFDND
                     KFGLLIADISGKGIPAALFMGTARNVLRAESRINNQPGQLLTHANKYIYEDSEYGMFV
                     TAFYMLIDSHNKLLTYGSGGHNNQLLVRSRSKETIHLNAKGIALGIINKAQYEEKALF
                     FEDNDLVVMFTDGVLDLLGNGNMDEGEKKLIDYSLQSLEKGPDFLISRLKNNLIVQDV
                     DKEFLDDFTVLAIQF"
     gene            17250..18230
                     /locus_tag="PLX22_01325"
     CDS             17250..18230
                     /locus_tag="PLX22_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOT18573.1"
                     /translation="MKKARYIFIIIGILILLFLFKTFGIQKTILQIISVGWGFWIIVS
                     IFIISHIFLTLGWKVLINHPIEGNTFLKLVLARIAGDSTSSINAFGSIAGEPLKAMYM
                     KDDIPLQIGLASVVLDRTAHSIANALMFLTGIVLSLFILNLPFYITIIALSFFIVTIA
                     IIIIIIKKQREGLLDYIVSKIPSRLKTKLITNERNKKIAELDNEIKYIFSSTSHVSRF
                     IASILLHYFAVTISCTLEIYLIVYFLKIPFNIYHALFLYVFGLFLTSVMFFMPANLGT
                     SEGSYSLALKLLGYDPSLGLTIGIVRRLRTFVWSLIGICILVIAGFKKND"
     gene            18356..19276
                     /locus_tag="PLX22_01330"
     CDS             18356..19276
                     /locus_tag="PLX22_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOT18574.1"
                     /translation="MSVVSPFARFFIKRKQAKAFPHKKVLVLEGGGMRGVFLTGVLQA
                     FTDRNYFPFKLIIGSSAGALTGVAYAAHQIYLARDAFFGVLLTDSFIKITNILRPAKH
                     ILNLDWMVSAVIEGDDALNCNYLQHSIPVIITATEYNDNNPPGTLYLHSKKDDLIPSL
                     KASAAIPVLYRGFVEYKTHKLCDGGMLDPIPYKKALAMGYKEEDIVVVLTRSKGYRKK
                     EESFWIRYIVENYFKEAKYRYLVQALENRYLMYNTLLDELEHKHKKISIIYPPDDFTV
                     DRLTRDHKKILQGFEQGVQAGKNFLLSSSL"
     gene            complement(19282..20319)
                     /locus_tag="PLX22_01335"
     CDS             complement(19282..20319)
                     /locus_tag="PLX22_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOT18575.1"
                     /translation="MRKMINVFTIFMMPLMCVLLLIHNAYGLQVSFVVGEAFVERNGK
                     QLTATVGMNVDTGDTIITKKASIVELLFLDGSAVKIFENAKVKIARVQEKEENISVVN
                     GVVKAKIAKLQKNESKKIYTPTTVCAIRGTEFFVAVSDSSNSRINMEEGSLELINPYG
                     ALTIQGDEQANAEIAKKAEADENLKNPEAWKNTEDKNFTIEPQKYADSYEEYLQTLEE
                     RSSTQSAEIAKLKKMPIKKNYSDKIEQDMQEVEEKAAMIADDYFLGDCASAAVETVLQ
                     NYKDKKQEIYDAFDKIKSQADRVLQQQKANYEAIMAVKEAYRKSYEEIKGKHKQSIEQ
                     IKEEVKKVQEQ"
     gene            complement(20625..21827)
                     /locus_tag="PLX22_01340"
     CDS             complement(20625..21827)
                     /locus_tag="PLX22_01340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18576.1"
                     /translation="MNAVEKPEVVIIDDDEMVNEVLKFILDDEYRVYNYYTAEDALRS
                     DVIMQADVIVTDVNLPGINGLEFIQKVHEINESIPIIIITAYNDIEIAISALKAGAFD
                     FILKPFKNDQISLAVKRAYERYKLIKENINLVGELKLKNNELEKLYAQLQARSVQIEN
                     ELDIASNLQQCLFPISFPEIKGFSFYLKFKPVEKISGDFFDFIIHDDQHFSFIFADVS
                     GHGVPAALYSAIVKTAISSIVTHEKSPSEIMNEMNQFLIQTQKTMSYNYAAIFLAHIN
                     LDNHRIEYINAGLPAPIVIHNNGDAIRLETTGPFVGIFDTSLYTAKSVKLLAGDKLLF
                     FSDGAFECPDKVDRILGEKQFQRLIDEHSKMEINELIEFLFKRVQEFCGYDRFVDDVT
                     MLGMKYEP"
     gene            complement(21828..29210)
                     /locus_tag="PLX22_01345"
     CDS             complement(21828..29210)
                     /locus_tag="PLX22_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18577.1"
                     /translation="MKRLLFIVLLLLLSFAGCVSTAQRDVPFDYSQVLTKEYRFGTGR
                     VIPLTFERSVNVSGTITTDGKYFIYASNRERNNFDIYLRPLNDITTFRLTVHPAKDFA
                     PDVSPDGKWCVFVSTRDDPQGDILLMKLSIDTILSQSKLEGEQPAQNLTTIKDTATGA
                     IVPIRDASPCFSPDGKWIAFSSEREGEESIFIMSRDGNKVRKITSGMQPRFSNDGNAI
                     LFTKQDGKETNIYRITIATGAIQKLTNNSSLQLSPCYGKTDDDIYFTRVEHDTNSDGV
                     VDASDNGMLYYYNVITGEEYPLTLIDEYSTAPRWVPYADGVIVYSVVEGEHINMAMIP
                     HTGIIPKQKNALRQYELALKYKEDFDDIERYQRCLFATYYFFREAVDIDSQMIIAKAL
                     YELSRDEHYTIAKQILQELSPKSKPASLYAQFILLKENNTITYLEKNIQELKLENNEK
                     LTPFFIEELADRYAAQGFTQRALTTYRDIVDTYATYSRLAYVRFKIGRLLYGGFTIPE
                     EWIVVLGSSYTYLKNEITMEVINATKQLPAAQRYAGAKEAVERYTLPIAKAIFQYIAA
                     TSLDELGQNEEAWLYLKQGLANTKKLDVAYFLINVKSGELAQKQKSDDWAGYYEAATN
                     NFQVQWKQDIRPIIEQLTEYFETNGQKLYEEGKYKESGELYKRYITMNTLLYLRRRYQ
                     DVYNINAAGAHIGYINASIPLQNTMLDTLKDEYSGQGHRLSVARMDFDKAHIYALGYI
                     YVQEALTLGSSPHAMEGRTSGELVKQLAMLKQALYHIDWALFIDDRFVDAYILKGWVY
                     QYVDELRMCCPSKKRLIDSYFSEYLWEQCIPLYEKALEANDEIITPRKEADIHTNLAN
                     IYFLLKNYTLALPHYNKAVSYQKAFTSLKEEAQFYYHYGYCLWQQGNYSGAYAAMQRA
                     YTIYKAIAQKNELSVKDQLYTLYKYFALFQQMQGNYTKAIDWYTTCVIHAARYNHEES
                     NSRLYIAIAECYRKLGNDKEALTYLELAEKNVSLTKEKQYKIGLKIVTLGPVYVFTVN
                     QDDIIIGEGKIVSELSSFEMQLLIHSMTAEIYISNGRFTDALTRYDKIIKLAGNRKGS
                     LFLDVTFSALQNAGYCAAQSGQIADAVRYYEQALAIAQKESIAVDKVYRMMLQWAYCY
                     TAGSYGAKDALHQTDVALDTIEHYTTSMYERVYAQLSNNYINTMKAQKKRPVPEDIEK
                     LSQQATKQVEGSTISFNALYALLLMQKATIMANNQSSLGQSTATASLYSQASALLSQA
                     IDYNANDPYHQEFVIKLLINRGICNIATGKVKEGYDDIISARFKANAVQSKELVWFAE
                     ATVWDYANRYNSLMQHIENPDSLIAGVLSRVELLPQLYARYAGFIYNLYDRYTDYLLN
                     AGKYREAFTAQQRKQIVALAMSGATLHDFYNKADVEFFTHYKKTCFTVYKLERALTQK
                     IVADGKKELIDTIKENLSMQYKKIDTFRKNATDFMAPYVGAYRATRYDVPVLHFYDYN
                     GKLYCWIAYKNTVDWRLISDEINAKTITADIEAVLQNYGNRGYVILNDSFMTIHPLLD
                     TTLLYKLGCIPCAARAPEVEKKVWLPVTSIATDMRLSGIPNVINDIHEAEYIVDSENK
                     LYAVTARYCFTTVIECSAVVKNIQGLSLNDLLLLSDAALYADVATIIGYYAGGRVETI
                     VRAISSDDANLLHEVNTPLLLLGKTPSDIKQRESNALVVAKSSSQLFNNALGKGDYTK
                     ALLALERWKQTGLHDNEYTIHKIKVLLYQGKIDDAVALIQLIADNDSIARSYLVYAYL
                     LQGHMEEALKALKGNAALQGTLDNEVYAALIEAWNGNQPLAEYDTFDNHEKSLLPLNQ
                     LRLLYIQFAALLKEDDKAGKALQQFTADYILSQRELCLVYSLGYAIDSYPIATNAGML
                     INSLKNNKYSVDTLTSMDYDALSLFVIHEIVLHADINEEAWLKMLMATKNALQYAPWV
                     DIQFYTIKIADNCNKSVPQRSIQLIESIPSQKGKPAWTFVLQHIVAYQKTGKFKQAHT
                     LCTAFHDLPFQIKRQWYMRYLYSVIAMGKTKEAGKLLDESGMISWDKSLYDLFLMNIQ
                     LQKMAIAKKTDESVFKAAENAIARFIVSLSSDMAGFYYSDYPYLLDKLFNYMVSYSMS
                     RGNQKDALQYAELHRMIYGIAVLSNNEYFKQQYDNLVRNAIPITAIQKKLPKDVALLY
                     TAKNENDIFAWIITRTYIQPVRLSNCYSEVKDLVYEYYNNLGLLQPVGGIENNIDEKM
                     KPVIKECAPYSRVIIFADVYTDAIPFEAILYHQSGSVVAFMPSLVAAMGSTRVIPSVI
                     AINIGDSNRDSIIAAVKQSGVHYGFAISSSIIITDKPLWYSDNKKNFVMDDALVKPGC
                     LIIDLTIMQGQSYNTGLVLMQKGIGGFITFNCAVIDVNRLLFLREFLTALKSKPSFTA
                     YADAFVYVIRDKRYKHPANWVGIRFYSSSMNYLYKGAD"
     gene            complement(29207..29806)
                     /locus_tag="PLX22_01350"
     CDS             complement(29207..29806)
                     /locus_tag="PLX22_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOT18578.1"
                     /translation="MKKNMVAIALACIVCIVIVMSGCTSSGCIQDMPSCLEIYPPAIN
                     ITGEKTALERQLIGEYHELEQDAWVTSTVMTPSKGNRKAVVNADDELLNAIQIRELNS
                     DTIRLYKDKELIGEQNNGYIAIVDEKKLQSLQEADVIRTVVQQENNARSVIFVKSSGA
                     ARSSEAKAIQFAVEFAKEQHAKAKKGDWIQNNDGRWVKK"
     gene            complement(29803..>30348)
                     /locus_tag="PLX22_01355"
     CDS             complement(29803..>30348)
                     /locus_tag="PLX22_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18579.1"
                     /translation="KRNTLEIKNVQATVMGGTVTLKELLFNLADLKPENMGFRVAFNV
                     NDIDIGKLDDPSSLRIHHDSLLSMNAIMQGKNLSTGQNLDISGTATIYKVGEQFANRL
                     FKGLNEEKGKSKLGMAQIAVDNSLIIAGFDFYIDKGLVYPTVVFKRKILGALVTVNNE
                     RVRYERIPVMEFFNKVREESL"
BASE COUNT         9034 a         6302 c         4986 g         9926 t
ORIGIN      
        1 tattaattct tttaatctca gacaacgatt cgcagttaaa catttgtatt ccggttttaa
       61 gggcatattc aatttcctct acgtttttac caacacctgc atacactatc ttttcaggat
      121 ttacgcctgc ggcgatagct ctatacaatt cgcctcctga aacaacatcc ataccgcacc
      181 cgacatcttt catcagcttt aaaatgctaa cattggaatt tgacttcacc gaataacaaa
      241 tagtatgtgc aacagatgct aaagcttcat ccagcagggt aaacttatca ataaaacatt
      301 ttgatgaata aacgtataaa ggcgtcccat atattcttgc cacatcagca agggaaacat
      361 cttcacaata taactggtca tctctataaa caaaatattc tctgaaagag ttcattgatt
      421 atacttctca attgtttcaa tagtatattg cttcatctgt tcactttttc gtatttcaac
      481 cttatcacca accttcttac ctaacaatgc ctgcgctatg ggtgatcgat acgataatat
      541 tcgcttttca aaatcagcat cccatggtcc cagtacagca aatgtttctt tttcaccatt
      601 tccggatatg gtcacaacag taccaacagt tactttatca agagaaattg aattcatatc
      661 aagaatctgg gctttcttaa tctcattttc aagcctgtta atatctcgtt ttaaaattga
      721 ttgtttttct aacaatgaat tgtattcaac attttccctc acatcgcctg ttgcatccgc
      781 cactttagca agttcttttg aaattgtagc taattcaata tttatcaatc tattcaattc
      841 ttcttgttta cgggcaaatc cttctggagt tgtaatgagt atctctgcat caattctcca
      901 ttcttcttca ataacatttt gctgtgggtt aaattcaggg aatcgcgatt ttattatatc
      961 cagaaacttt tccctcattg aatcatcaaa atacggtatg ctggcagcaa tatcaaatat
     1021 cttccctaaa aactgcacat caaaattgtt tacaacatcc tttaatacag cagcatcacc
     1081 gccaaaaagt agttctattg ctacattttt tagtttattt cccttcagct caatcttctt
     1141 aagttcattc attaatctaa acaatgttaa cacaagctgt tcacgggaat aatctaacca
     1201 atcataatcc catgattttg taagcaaatt tttggctacc cataaaaata tttcagggaa
     1261 ttcttttgca ctaataagtg cctgttcaac aaatttattt ataatcccat attcatgcga
     1321 gcggattaat atatttacta tatacttatg tatgcgcacc ggagtttcaa acaaaacccg
     1381 tccatagata tccgcccaat cttcgcgtgc atcctttatg agatttataa aatccttttt
     1441 ataatcatat gagtctatct ttttggaaat agagggtata tctggatact ccttcacaat
     1501 tgagactacc atcttatatg cctgatcaag tttcaatttc tttgcatcta taaatttgct
     1561 caaaccactt aaaataaagt atgataaaat ttttcttgtt accgaatcac ctttcagttc
     1621 ccctgaaaaa taatccacaa agaattgagc aactgctgct ccttcagatg gttcaatatt
     1681 gttaacaaat tctatagcta cattcagctt atcagtaaat gaatccgttt tcaggaaatt
     1741 atcaagcaac tcatcagcaa aggtaagagg tttatcacgc atgataaaca tgtccttttt
     1801 ttctggtgaa attccaaata atggggattt tcgtatttct gatcttttgc tgctccacca
     1861 ttttgcccag gttttttcac taacaaaatc aggaatgagc tctttcttaa tatcagcaac
     1921 ggttattctt ccattatacg acttgattaa tatctcaaaa aattctaaga ggtcttcatc
     1981 aaaaagtttc tttaatcctt cataatcttc acactttagc acctgaatat gatcacttct
     2041 aagcggcgtt agagcctgta acgccatctg cacagacatc tggtgctcct gtttttcttt
     2101 aaaatcaatg acaatagtgt tgggtgtaat atcaattatt ctgccaatac cccagcttct
     2161 atgccgaaca taatgtcctt tatcaaaaat gatactattt tcaaaatcct gtatagcatg
     2221 cttcacagga tatttgaaat tagtcagctt tgacaatttc aaaaatagct gaaactgcga
     2281 atggctgcca tatttctttt catataattt aacaagttct cttctggcgg cagtgtcatt
     2341 tggtgtatat tccagaattt ttttcaatat ctgtatggat tgatcaggat gttcttcatc
     2401 cttatactta tgaagtaata cttttaacaa tgaagaagtc aactcatact gccttgcttc
     2461 tatcagcatc cgctcaattc tttcaaaaaa tggcatatct tcccatgata cctgaaccaa
     2521 tttgttccat aagtcgctga tttcatcata aagcccattt tttatatggc cctcaataga
     2581 tagtttcata tattgaatgc ttttttgctt atcttcatca ataagtgcaa ctgcaagttt
     2641 ttttgcaacc tcagcatcaa atctatcgat ctttagtaaa ttttcccaaa cagggattgc
     2701 ttctttactt cttcccaacc gttcaaggct tgttgcaaga gctttcaatg caaagctact
     2761 ctcaccgtat tccaggactt tttcagacag cagctctgca atgacccatt tatgatattt
     2821 ttgatacatt tcaattaatt tgcgcatctc aattttatca tcaataatat caagatgata
     2881 tccggttagt ccaataagat atgaagcaac aattgaatcg ggatgggaaa caatatgttc
     2941 tttgcacata ttccttactt cttccaatac acccgcatca acgactgaat tgaataggtc
     3001 gtcaagtatt ctgaatctgg atattccaac atctttggga tccagcctcc cccacatttc
     3061 ttctttaaac attgagctta cacgctcaaa tattttctca ttttccatat ttgcctcttt
     3121 tgaatcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct tcaggatttg
     3241 cacgaatcac aaaccgtaca atttttgtta ccctggtttg agggtatgaa tttgtttcct
     3301 gaggaacata ttccggaatg tacgctttca tgggccgcca cccaacaaaa tttaatgaac
     3361 ccattttcaa aacatggaca tcgcctttat aatcctttat ccacacatct aaggtataat
     3421 tattccctct ggaatggacc cacatcgata tagctttagc ccttccgggt aactggattg
     3481 ctctttcctg tatatctttc cctgaacggg aatcataggt caatactgct tttcctggac
     3541 tatcccattg tacttctaga ggagggagta gatgaacgga gttaaaccca ggatatctaa
     3601 atttaaagtg aacacctaaa cattttttct tttctaatcc cttattaatt gggctccact
     3661 tttctgtaag tagatctgca gggccaccat caacatattt aagctctaac accggcacag
     3721 ggtctttttt aggatcagga ttcttttttg gaattgtttc caattgccaa tcagcctgag
     3781 tttcaaaatc ttctactacc agagcactat attgctctgc actgatatcg ggttccacat
     3841 tggttactat cctggattgt actttttcag ggaaaaatcc cacaagcacc atcccaacaa
     3901 taacaactat aattgctgaa aatcgtatat atctcatatt tatgcctcca ggtctcattt
     3961 gctcatcatt ttcattatca ccagttatcc ctgatgcttg tatcaccagt gaactctgat
     4021 agatcagtaa ttactctgaa attatccagg tagaaataaa acgtccctgg tacttcataa
     4081 atgtcacttt ctacatagaa ggaaacaaaa tggagatttt tatcgagcaa tgcataccct
     4141 ggactctgtg gcaaccaacc cggtacaatg actgacatct ctctccatcc ccagaaatcc
     4201 aatcttccaa tttttatttt gtgaatggct cctttataat cgcgtagctt cacaaaaagt
     4261 gtatgcctga attttcgtcc taatgcccaa accttgatct cttttgcttt accacgaact
     4321 atatactcat ttgggggaaa aacctcaact ctatcaaaac ctctatccat aaaataagtc
     4381 ttaacaccta atacatattt attctcatgt gtgattccat tttcatcagt tatcgtatca
     4441 ggataattaa cctcattgcc attttcatca attggtcttg gtttaccggg aatcttcctg
     4501 atttttgttt ctccaattgg acatgttgcc attgcccgcc aatcttcact tgcttcaaag
     4561 tcatccatcc aatcttcaac taaaccagtt tgttgctttt tctgttgttg tgcctgctgc
     4621 gcttcctgcg cctgcaccac tatattgcct ccaccgactg ctaccattaa accaccgatg
     4681 gcaagcgaca atataaaaga tctgacctcc cttctcattg tcgactcctt tttaaaaaga
     4741 ttatgaaacg tttaatacct attaatagta tttgcttttc accataatac tatgatatca
     4801 tatacttaat cggctaaaaa agtaaaaata tttaataatt attcttatta tgttcgatag
     4861 tatctgatat tatatcgtta ctgtcaagaa attaatattt atttattgct atcaaaaaaa
     4921 atttatacac aattgttatt gattttttta ttgccacaaa tcactatcac cataatcaac
     4981 ttatcaccat ttttctatat taaaaattaa aagtgagtgt tatggatatt gctgtctgtc
     5041 tacaacataa aaaaccattg ctgtcatttg aagtgtttcc acctaagact tcatcagggg
     5101 aggaaaatct cttcaaagaa ctatcgatca tggtgcacta taaaccagac tttatttcag
     5161 taacctatgg agctggcggt tcaacacgag ataaaaccct tgagattgcg attcgcataa
     5221 aaaaatcatt ttcaattgaa ccacttgttc attttacctg tgttggccag agtaaagatg
     5281 acattgtttc ttatcttcat tatgtgaaaa cactgggatt tagcaatatt ttagccttgc
     5341 ggggcgatcc cccaatgggc gaaacccatt ttaaacctca tcccggcggt tttacccatg
     5401 caaacgaact ggtatcattt atcagaagta tgaatcactt taccattgga gtagcaggat
     5461 atcctgaagg acatattgaa gcacccgatt tagataccga tatagccaac ttaaagaaga
     5521 aagtagatgc aggagctgat tttatcatta cgcagctttt tttcaataat gataaatttt
     5581 atacattttt ggataagacc ataaaagcag gcattactat tcccatccta cctggcataa
     5641 tgccattaac cagtattgca caacttacta aaatcacaac aatgtgtaat cctgatattc
     5701 ccaattcatt attacaggag cttgagaaat gttctcagcc cgatgatatg tgtaaggttg
     5761 gtattgaatt taccatacag cagtgccagc aattacgttc gtatggagtt ccgggttttc
     5821 atttttatcc cttaaataaa tctgctgcaa taaaattaat tctggacgag ttctggcata
     5881 cataacacat tcagagattt ttttatcacg tggtaaacta tttttactat tttaaggggg
     5941 caaaaccatg aaatcctatc cattttattt ttatacaggg ataatgctca tagcttttat
     6001 gcaactatgc attctatttc atgtttcgca tgttacaata tgggctacgc caaccctgtg
     6061 gacaggtctt atcttactta ccgatggaat acttgccaaa agcaatcgct cattaataaa
     6121 aaaccgaact atcgtaaagc tatcactgtt atcatttctt tgctggtgga tgttcgaatg
     6181 gttcaatatc tttttatcta attggcatta tcagggactc attcaatctc tgcctatccg
     6241 atattttggc tatatctggt cattcagcac tatcttcccg ggagtattgc tggtatatgc
     6301 aatcatagca accatactgc ctaaactttt ttttaaacct atacatataa catcatcgtt
     6361 ccttgttgta tcctttatta ttggactatt ctttatcttt ataccaatca tccctttttc
     6421 tatgcattat acaaaccgtg cagctgaccc cgatctattc ttctggctta catgggctgc
     6481 caacattcat tatagtgagt ttacagccgg ttttacctgg ttaggattta tctttttact
     6541 ggagccagtt aattatttac taaatcgtcc ttccatctat gctgacctta tagtaggcaa
     6601 atacacgcga ctcgtgtctt tgttggcagc cggtttgctc tgcgggtatt tatgggaatt
     6661 ctggaattac tgggcagcaa caaaatggat atataccgtt cctattttac cctccctaaa
     6721 attgtttgag atgccaatcc ctgggtatat tggcttttta gcatttgcat gggagatata
     6781 tgccattact gcacttgttt atcctaaagc actatacacc ctggaaggtg aacgttaatc
     6841 acatggaaga tattattgtc attggacata aaaaccctga tactgattca atctgcgctg
     6901 catactgtta tgcacaatta aaaaatagtg tcgattctac caatcgttat cgggcatacc
     6961 gttgcggcaa tattaaccgt caaacccgtt ttgtatttaa ccaaattgga ttgcagccac
     7021 ctgatttaat caacaatgtg tatcctaaag taaacgatat tatgacaaaa aacgttatta
     7081 ctgcatatgc tgatgatcct ctctattcaa tcataaagca tcttgagcag gatagaatac
     7141 gtattatacc tatagtggac agtactaacg tgtttaaggg tattgtgagc tttaatgaaa
     7201 ttgctcaatt ttattttccg gtaacatcca tagcaaagcc agtttattta ttcaaacctc
     7261 aaaattttaa tcttaccatc ccgggacggt ttattcagca gggaaaagat aaggaatttg
     7321 aagcagcaat agttattgga gctatggaat caacacggtc tattgaagaa ctacacaatg
     7381 ttatccctga aaaaacggta ctcttagtag gcgatagaaa agagataatt gaatttgcga
     7441 tcaaaaataa ttttgctgca ctattgatta ccggagtgaa tgattttacc tatgacacta
     7501 ctcactataa aggatgtatt tatctttccg aaatagatac ctacaatacc ttgcgaatgt
     7561 taacgttaag cataccatgt cattctatta tgcaagatgc tgatacaata tccccggatt
     7621 tgccagtaga gcaggcacgc gaacaactgt tgcttaatga ataccgggga ctacccgtat
     7681 gtgacaatac tatcctgaaa gggattatta cccgttcgga cattatcaag cactatgcaa
     7741 aaaaggtaat ccttgtagat cacaatgaat tatcacaagc agttgatgga gctcaaaccg
     7801 ctcaaattat tgaaattatt gaccatcacc ggttgggaag cttaaaaacc acaaccccaa
     7861 tatttgtgta ttccaaacca gtgggaagta cctgtacact ggtttatgaa ctttttaaat
     7921 ataacaatgt taaacctgaa ccgcatatcg cacttctgct atgcagtggc atactatctg
     7981 ataccaccat gctattatca cccacaacta ctgaaactga caaaaaatca ttagaagaat
     8041 tggcagcgat agctggtatt aactacaacg attatgggca aaagctttta tcctcaaccg
     8101 aaagtttgaa aaccattgat cccatatatg cggtaagcaa tgattgtaaa atctatgaag
     8161 aatatggtat cgcggtagct atatcacagg ttgaggtgat aacacttgat ggtatcaatg
     8221 aagtgaaaga atcattgtta tctgcactgg tacaatttgc ccaaagcaga aatgttcact
     8281 gggcattatt attggttacc gacatcacca ctcagaatag catcctgtta tcaactgact
     8341 ttaaaggtga ggttcatttg aactatcccc ccattgagcc ccacatttat agtttgccgg
     8401 gcattttatc ccgaaaaaaa caattactcc ctgaaatatt gcgcatttta gaggaactat
     8461 cgcacaaata aaagtgcccg cttaaaacag cgggcactat gtttagtacg ggtattccct
     8521 gatagtagta tacgttttta tcttatgttc ctaaaagctg taagattgtt cttggtcgta
     8581 aatttgcctg agcaagcatc gcagtaccac tctgcacaag gatctgatcc ttggtcaaat
     8641 ctaccatcgt ttcagccatg tcagcgtcac gtatacggct ttcagatgcc tggatatttt
     8701 cataagcatt catcaaacct tttgctgcat gctccaatcg attatagtag gcacctaaat
     8761 cagcacgctg cttggctatc ttctgtagac cttcatcgat aataccgata gtcctgttgg
     8821 ccccttcagc agttgataga tttgctagaa attttccggt cttttctctt agatttaatc
     8881 ccagcgaagt catggttccg atatatacac gctcacgctg atgcatgttg gcacccatgt
     8941 ggaaccacat acttgctttt ggatttgttc ttgaaaaatc accaagcaag agtttgaact
     9001 tgttgaattc tgcctgagat gcaattcgat caatttcatc aaccagagcc gatacttcaa
     9061 cctgaataag ctgtctgtct tcaagagtat agataccatt agatgactga accgcaagca
     9121 ccctgattct ttgcaatatg gttgcagttt gatttaagta tccttctgct gtttgaacaa
     9181 atgacatacc atcttcagta tttcgctcag cctgccgcaa accctgaatc tgagtacgca
     9241 ttttttcaga tacagccaat cccgaagcat catcgcctgc tcggttgata cgcataccgg
     9301 atgataactg ctccattgaa cggttaacat cccagtgttt aaactttagc accttatttg
     9361 aattaatagc gcttaagttg tggttaataa tcatacacac actcctttgt gttaaaattt
     9421 tcctgatctc cctatcagga taaaaccggg ttgttgatct aaaaaaagag aatacccttt
     9481 ttttacttca ctacaacccc ttatccctca tttaagttgc taccttttta aaataaaaga
     9541 aaaaatgaag aatatggcgt tgcagggatt gccttattag ttttcaaaga accttacagc
     9601 ttattgtgat gttcggaagc tctgtttaaa gaattaaagg caattatatt tgtttatcta
     9661 tggtttatgg cgtttacggt tatttatact atattatgta tgcatttcat ctatttaatg
     9721 aatttttcta tatattttcc cttattcaac atttcggaat gtgaactatt tgctttagca
     9781 aattttttaa tttctattca tttcatgtaa tttttaaatc tatacgtcgt tgtctaccat
     9841 gaactttttt gaacacattg caatacgccc tttctgtcac tcacaatgaa gctttaacaa
     9901 tttgaccata aaaaaagaat aaacttgaca ataaatggtc gattattaaa atagacaggt
     9961 tatttttatt ctatacatgc aaagtcatgt atagaatatc tgctactttt ctatacattt
    10021 tgtttactat tgtttgataa ttactatact actatgcaac aggtttaaca atactatggg
    10081 attgaatccg tttaaagaat caaatttata tgatctccag aaacagatac aggacattgt
    10141 ctttgaaaaa tgggaacaga tcagttctaa aattaaagca aaatgggagc acataaagaa
    10201 aagaggaaat gagcgtttaa ccattatgat catccctcac tctgaaaaaa agatagtaaa
    10261 tttacacatt actcttttta cacttaccgg gattatcaca tcgatcgtac ttatattagc
    10321 gctcacatca gtatttatta taaaccatac atcaacaata aaagaagtat cccgtcttaa
    10381 gatgtatggt tcaaattcca aaatacagat tgcaaagtat aaagaagaag taaataaatt
    10441 atatgatata tttcagcatt taaaaccgga gctatcatac ctctattcat taacacctgg
    10501 caacagagcc gatactctct gggcaaaagg tggcgtatcg ggaatcgttg ataccgaaga
    10561 accgggttcg cctccaattg aaattctcaa tattgaagag atggtacagg atctggaaac
    10621 tactaaagat gttctcaaag acataaaaaa gttcatgcgt gaaagacgaa aagttataga
    10681 aaatacacca tcaatgtggc ctgttgatgg attcgttatt acccgctatg gtatacgtac
    10741 atctccattt acatttcaga aagaatttca tgaaggagtt gatatcgctg catttcccgg
    10801 agcacctatt aaagttaccg ctcctggtat agtggaatca atagtatgga acaatcaatt
    10861 aggattaact gtatctataa aacataaata tggatttgtt actgcctatt cacattgcca
    10921 gcgtgtgacg gttgaggaag gacaaaaagt tgcaaaaggt gaagttatcg ggtatgttgg
    10981 taaaaccgga aatgccacac gccatatctg cttttatcaa attaagattg gtacagaatt
    11041 tgttgatcca ttaccctatt taaacaaagt agtaaatcaa taagatattt gtaagtaata
    11101 agtaagctat tcctcttcac ttcttttgct gacaagcagt tcatcaggta tgtcttcaat
    11161 cttttctcct atttgcactg ctactctatt tcctatgaca cccgggcgac acttatattt
    11221 tttccttcca ccaatttttg ctgtcatatc tgaattaact ttagtgttgg ggagtttaat
    11281 aacatcacca agttgaatag ccaacaattc ttccatggtt atttctacct caccaacttc
    11341 tactattatg ggtaactcaa ccgattctaa cctgctttgt attattgcag cattttcatc
    11401 ggtagcaccc tttctgatac ttgaatacca gtactgagct gatagctttg aaataacagg
    11461 ttctatagtg atatagggaa tacataggtt catcatcccc tccacttccc ctacctttgt
    11521 ttccagcgtt acaagaacaa ccatgtcgtt aggaggaaca atctgtgcaa actgcggatt
    11581 tgtttcaata ttacccaatc gtggacgaag atcaatgaca tttgaccatg cttcacgaag
    11641 attccctaaa attctgacaa taatgccttc cataaccgat agctcaatat cggttaactc
    11701 acggctgatc tttgttgatt caccagtacc gccaaaaagc ttatcgatga tagtaaaggt
    11761 tattgaagga tctatctcca gtaccgctga acctttaaga ggatccatat tgataactgc
    11821 aagtgtagtg gggttgggaa tagaacgtat gaactcctca taggtaagct gatcaacaga
    11881 agccacatgc acacttacca aagcacgcaa ttgcgctgac agagcagttg ttgttaaacg
    11941 tgcaaacgtt tcatgcatca tctgcagggt acgtatctgg tctttggaaa atttatcagg
    12001 gcgccggaaa tcatatatct ttaccttgcg ctgttcttta accgcggtat aatcggttgt
    12061 atccgcttca cctgttgaga tggcggtcaa cagggcatca atttcatcct gtgaaagtat
    12121 ctctgtcata gattattcct taagtattga tacatctaca ttaactatca cccataaatt
    12181 gttatatggc tccagcgtgt accggtactt catcgttgca ggaaagcgcg gaccccatcg
    12241 ctttacctta acatcaacat aggcaacatt cgtctgcgct attatctgtt catgaagcac
    12301 tgaaaagtca atcaaataat cagggcgatc ctttgttata aaattaataa attcctgctc
    12361 aacaatcaag cgttcataat cgtttgtagt agaatagcgt tttgcaaatt ctgaataggc
    12421 ataaatatat ttttcaatat ttatatactt aataacatta tcccactttt tgttcatttc
    12481 tgcattgagg gttaacagta ccacctctga aggggtaatg ttacgctcct ttttaacaat
    12541 accggtacgc atggcaaatt ccggaaccgg aacaacagct atagtaagtt cattatctga
    12601 tttaatggta tactcatctt tagcatccgg ataaaagtat accttaatac ggtatttgtt
    12661 atcctttgcc aatgtataaa aattattgca cactgccctg tacacaaatt tttcatcagg
    12721 ttgcaaaata atttttcttc cctcttcttc tgctactaac ctgtaagcag gaatattctt
    12781 taaccggtaa tcaaccgtaa ttggtgcctc ttttccattc tgcaagtaca caactggctg
    12841 aaacgttgta tagggaatat catagatggt aaattcgacc ggtacctgtg cagtattatg
    12901 aacaacgcaa tacacaatga tgtcatcatt ttcatcgtat gatatctttg gacttaccag
    12961 cgttacattc agataatcag cactttctga ataaatgctc actgcaaaca gcaatgtggc
    13021 gatagttatt gtcaattttt tcatagtaat agcataataa aagtaagctc tcatgaaaag
    13081 gtagtacctt accgttttta tcgactacta taccgatatt tgttaccaaa atatatgttt
    13141 tgccagcgct ttttgcatta ttggaagcaa cttagctttt ggcctgcgat caaaggtaac
    13201 aaccccgttt gtttcctgaa atgtatcggt aaattgtgta tagcaaaaac cctgtatttg
    13261 tggaaatttt gataagatac tgcaatgctt ctgaaagctt tcttcaaggg tttttgcctc
    13321 cgaggggtat ataccaaaac caaacccacc catctcgctt atgataatcg ggcttgttgt
    13381 gtcgccatat cccttgagat aaggagtttg tatattctcc ttacccagaa tcatcataaa
    13441 aaaatgcgtc catagcggcg cctctctgac gccatcaacc agccgtttat aaagctcttc
    13501 aatatcttca aatcgctgca aatagtggtg tatgtcggta atatcgctct taagatgatg
    13561 aaatccacta ttgtcaataa taaggtaccc atggtattgg ctctttacgg tatcgaaaag
    13621 ctctactaca ctgttgcgca ccttccgtga aaaaaacatt gaatagagtc cccagctttc
    13681 attatacagt acaagcgtaa tgatggaggg gtgcatacag tcgcgcgcca taacctcctg
    13741 cagttgcgca tataatcgtt gcatattctt ttgtaatggg agatagtaac tgggcatctc
    13801 ttcccatatt aaaaatccta ccatatccgc ccaatataaa aaccgcggat cctctatctt
    13861 ttgatgtatg cggcagccat tgaatccaag cgatttcata atttcaatat cagatttata
    13921 ctgcatggta tccacaggac gatataatcc ttccggatag tatccctgat tgagcaataa
    13981 ccgctgatag atattttttt tattgagcag tatcctgcca ttctgtatgg taacatccct
    14041 cctgccaaaa tagctatgga tacagtctat ttctttcccg gcttttttta gtactacctg
    14101 aatgggatgt acggcaggat gtgaagggct ccatacatca aaaagcgaca gcggcacttc
    14161 aataacaacc tgtccttttt taaatacatc aaaaacaact atatcacgat acactttgac
    14221 aggggttttt aacacactcc gttttccaaa cacctgtgat gcaaagattt taagctcaac
    14281 ggtaatatcg ttacggacat tctcctggct ttcaacgctc atagcaaagc aaacagcagc
    14341 atctctggta catgtcagtg atgttatatg tgtgctggca acctgttcaa tccacacatc
    14401 ctgccatatt ccgctactat ttgtatacca caccagaaat ggcttgtgaa gaaaagtctg
    14461 tttgcctcgt atctgtgatg gcgatatgct atcctcaaca agcacaacca gatagttgat
    14521 atcctcaaat tctggcacaa aaaacgaaaa aggcgtgtat cctccttcat gaaaacccag
    14581 acatctgcca ttaagccata ccgttgcttt ataatcgcat gctccaaaat gcaatcgtcc
    14641 actttttata gcatcaacac caaaggaaca aaaataccag aaacgctttt gtttaattat
    14701 ttcatcaggt gccatgtgac tttttacatg aattgcgctg ttcacatctg cttcaagagg
    14761 aaatggtacc tccacagggt atgactcaaa tcccttaagt atatagtgcc cgcttataat
    14821 ctttcctgca ttttccttgt tataccattg taaacgtata ccacgattct gtttatcaaa
    14881 tgccataatc cattgcccat gtaaattgaa tgaagggagc cgtatgcaat caggtcgtgg
    14941 aaaatggcct cgtccttcaa tgctgtatat gttttttaat gtcaactctg tcggatccat
    15001 cttgttctcc tgaaacagta ttgtttctca atgtcattcc gtgcctgtca cggaatccag
    15061 atgtgtaaca gtatgtcctg aaatgatcct gaaaccagtt caggatgacg ctcctcacat
    15121 tgtcatgctg agggacacag tcccgaagca tctattcgtt cacaaaacgg cgatattctt
    15181 cggattcgct tcagaatgac tatcctctca caatcagcat accattagct aacgacaaat
    15241 ataatcatgt aatactatcg cacctataca tttttttcat ttacgcaacg ctgatgcatg
    15301 ctgcaaatac ttttttatgg aagttatact ctaacataaa aaacatgctt ttactgtacc
    15361 attatactaa aaagccgttg tggtaatcaa atctattttt aatagtaact atcgaaaaaa
    15421 gatagacatt ttttaaaatt atattctaat agtactctat attaaataat taatgaatat
    15481 aattacttat gaattacgat atcgagcaat ttagcgcaat taataagagg attaactcaa
    15541 gccttaagct atctgatctt ttaaccataa tccttgatac aacaaaagat atgcttaagt
    15601 ctgaaggatg ttctatactg cttaccgacc ctataaccga agacttatat ttttatattg
    15661 tcaggggtgg cggcgattca ctcataaaag gggagatagt tcctaaaggc aaaggcattg
    15721 ccgggtatgt tgcgcttacc ggcactccac ttattgttaa caatccctac aatgatgagc
    15781 gcttctacga agatattgat agtaaaagca actttcacac gagaaatatc atatgctcac
    15841 caatgatact cattgacagg ctggttgggg ttatagaggt tgtcaacagt accgaaagag
    15901 aaagtttcac cgatgacgat cttaaagtgt taatgtatat tgcagattct gcagccattg
    15961 caataactca ccgacagctt taccatgacc tggcaacacg gctaaaagaa cttgaaatta
    16021 tgtacgaaat atcacaggct atagcacatt ccagcccatc agaaaatatt tttccaaaga
    16081 ttatgcaacc gttagcatcg tctgttggtg ttaagcgcgc atcaatactg ctgtataata
    16141 caaataccga tgagttttct attgcagcct cacacaatct tccggaagct ataaacctgt
    16201 atgtaaacaa ggataatgct cccattgcat accgcgttta caccagtggc gaccctctac
    16261 ttgtcagcaa tatcatgtcc gatccttcat tagcaagttt tgccagaact catgaatata
    16321 taactccttc gtttatatca ataccactgc gatataaaaa taccattatt ggtgtattaa
    16381 accttgcgga taaagtggac aaatcaagct ttgatgctca tgatctgaga gttgcaacaa
    16441 ccattgctaa tcaaatcgta gaagcatacc agaattccat ctatttacag caaactgagg
    16501 aacagcgaag gcttgctcag gaaattgata ttgccgctga aattcagaaa cgaatattgc
    16561 caaaaattcc tcactctatc aattcacaca ggcttgcagc ttttaataaa tcagcaaaag
    16621 agattggtgg agatttttac gacatttttc agtttgacaa tgacaagttt ggtttattaa
    16681 tagccgacat ctccgggaaa ggtattcctg ccgcgctttt tatgggtaca gcgcgcaatg
    16741 tattgcgtgc cgagtcgcgt atcaacaatc agcctggaca gcttttaacc catgccaata
    16801 aatatatcta tgaagattct gaatatggca tgtttgtaac agctttttat atgcttattg
    16861 attcgcataa taagcttctt acgtatggca gcggaggaca caataatcaa ctgttggtcc
    16921 gctcgcgcag taaagaaaca atacatctta atgctaaagg catagccctg ggtataatca
    16981 acaaggcaca atatgaggag aaagcgctat tttttgagga caacgattta gtggttatgt
    17041 ttactgatgg ggttcttgat cttcttggga atgggaatat ggatgaaggg gaaaaaaagc
    17101 ttattgacta ttcattgcaa tcacttgaaa aaggacctga tttccttata tcccgcctta
    17161 aaaacaatct tattgtgcag gatgttgaca aagaattttt agatgatttt accgttcttg
    17221 ctattcaatt ttaataatct ggaaatttta tgaaaaaagc ccgttacata tttattatta
    17281 ttggaatact tatactgtta tttcttttta aaacattcgg catacaaaaa acaatattgc
    17341 aaattatttc agttggatgg ggtttctgga taattgttag catttttata ataagccaca
    17401 tctttttaac attagggtgg aaggttctta taaatcatcc tatagaagga aatacatttc
    17461 ttaaacttgt actggcccgc attgctggcg attcaacatc atcaataaat gcctttggct
    17521 ctattgcggg tgaaccattg aaagctatgt acatgaaaga cgacattcca ttgcaaattg
    17581 gtctggcatc agtggttctg gatcgaactg ctcattcaat agccaatgct ttaatgtttt
    17641 taacgggtat cgtactgagc ctgttcatac tcaatttgcc tttttacatt accatcattg
    17701 ccctttcttt tttcatagta actatcgcca taattattat tattattaaa aagcagcgtg
    17761 aaggcttgct ggattacatt gtttccaaaa ttccttcacg gttaaaaact aaacttataa
    17821 ccaatgaaag aaacaaaaaa attgcagagc ttgataatga gataaaatat atattcagca
    17881 gcacatcaca tgtatcacga tttattgctt caatcctgct ccattatttt gctgtaacca
    17941 tttcatgtac ccttgagata tatcttattg tctattttct caaaatacca tttaatatat
    18001 accatgcatt atttttatat gtctttggtt tgttccttac ctcggtgatg ttttttatgc
    18061 ctgccaattt aggaacaagt gaaggctcat attcactggc attgaagttg ctgggatatg
    18121 atccttcgct gggacttacc atagggattg tacggagatt gcgaacattt gtctggtcac
    18181 ttatcggtat atgtatactg gtgattgccg gctttaaaaa aaatgattga gcacccctgt
    18241 ttcagaaatt actttatttt tttatccatg caatttttta tggttgacac tgctctccat
    18301 tgcattgttt taataataac aaaataaaaa acacttgttc aggaaggagc atctcatgtc
    18361 agtggtatca ccttttgcac gtttttttat aaaaaggaag caggcaaagg ctttcccgca
    18421 caaaaaggtt cttgtacttg aaggaggcgg gatgcgaggc gtttttttaa ccggtgtttt
    18481 acaggctttt accgatagaa attatttccc tttcaaactc attattggct catctgcagg
    18541 agccttaact ggcgttgcct atgcggcaca tcaaatttat ctggcacgcg atgccttttt
    18601 tggtgttttg ttaaccgata gttttatcaa aattaccaat attctgcggc cagcaaagca
    18661 tattttaaat ttagactgga tggtttcggc agtgatagaa ggtgatgatg ctttaaattg
    18721 caactattta cagcattcta ttccagtcat tatcacagca acagaatata atgataacaa
    18781 cccgccaggc acactctatc ttcattcaaa aaaggatgac ctcataccat cattgaaagc
    18841 ctccgctgcc atcccggtac tgtatcgagg ctttgttgag tataaaacac ataagctatg
    18901 tgatggtggc atgcttgatc ccattcccta caaaaaagct ttagctatgg gatataaaga
    18961 agaggatata gtggtagtat tgactcgtag caagggatac cgtaaaaaag aggaatcatt
    19021 ctggattaga tatattgttg aaaattattt taaagaggca aaatatcgct atctggtaca
    19081 ggcgctggaa aaccgttacc tcatgtataa tacattgctt gatgaattag agcataaaca
    19141 caaaaaaata agcatcattt atccaccaga tgatttcact gtagacagat taacccgcga
    19201 ccataaaaaa attctacagg gatttgaaca gggtgtacag gcaggcaaaa attttttact
    19261 atcgtcatcg ttgtaatact attattgctc ctgaaccttt tttacttcct ctttaatttg
    19321 ttcaatactt tgtttatgtt taccctttat ttcctcataa cttttacgat atgcttcttt
    19381 tactgccatt attgcttcat aatttgcttt ttgttgctgt aataccctat ccgcctgtga
    19441 ttttatttta tcaaaagcat catatatctc ttgtttctta tctttataat tttgtaaaac
    19501 agtttcaact gcagcacttg cacaatcacc taaaaaataa tcatcagcaa tcattgcagc
    19561 tttttcttcc acttcctgca tatcttgttc tatcttatct gaataatttt ttttaattgg
    19621 catcttcttt aattttgcaa tttctgctga ttgggttgaa cttcgttctt caagcgtctg
    19681 taaatattct tcataactgt cagcatactt ttgcggctct attgtaaaat ttttatcttc
    19741 agtatttttc catgcctccg gatttttgag gttttcatct gcttcagctt tttttgcaat
    19801 ctcagcattt gcctgttcgt ccccctgaat ggtgagcgcc ccatatggat taatgagttc
    19861 caacgaccct tcttccatat taattctgga atttgaacta tcgctaacag ctacaaaaaa
    19921 ctcggtacct ctgattgcac ataccgttgt tggagtatat atcttttttg attcattttt
    19981 ttgcaattta gcaatctttg cctttaccac accattcact actgatatat tttcttcctt
    20041 ttcctgtacg cgtgcaattt tcacctttgc attttcaaaa atttttactg cactcccatc
    20101 aagaaaaagc aactcaacaa tgcttgcctt ctttgttatg atagtatctc ctgtatctac
    20161 attcatacca acggttgctg ttagttgctt accattgcgc tctacaaatg cctcgccaac
    20221 cacaaacgat acctgtaaac catacgcatt gtgtatcagc aataacacac acatcaatgg
    20281 catcataaat attgtaaaaa catttatcat ttttctcatg gtattctctc caaaaataaa
    20341 tttatgtgac tatgtcatgc tggattcagg tctgtcacca taacaggctc gtttcagtat
    20401 ctcttattca ataaagatcc tgaatcagta ttaaggatct atcctttgaa cagttagtac
    20461 actacaacat atcaatcgtt gctaaaaact ctattgatca tattccacta caatatgcat
    20521 gccactatca ccattgcaaa acatggtggt atcatttaat tatttacaat acttttcata
    20581 aaaagtgcaa tttatttatc gtgttgctac ggttatagtt aatactatgg ttcatatttc
    20641 atccccagca ttgttacatc atcaacaaag cgatcataac cacaaaattc ttgcactctt
    20701 ttaaacaaaa attcaataag ttcattgatt tccattttac tatgttcatc aattaaccgc
    20761 tgaaactgct tttcccctaa tattctgtca accttatcag ggcattcaaa ggcaccatct
    20821 gaaaaaaata aaagcttatc accagctaat agtttaacgc tttttgcggt atacaacgat
    20881 gtatcaaaaa tgccaacaaa aggtccagtg gtttccagac gtatagcatc gccattgtta
    20941 tggataacaa ttggtgccgg caaacctgca ttaatatact caatgcgatg attatccaga
    21001 ttaatatgtg ctaaaaatat agcagcataa ttgtagctca ttgttttttg cgtctgaatt
    21061 aaaaactgat tcatttcatt catgatttcg gatggagatt tctcatgggt aacaatagat
    21121 gaaatcgccg ttttaacaat ggcagaatat aacgctgcag gcacaccatg cccggaaaca
    21181 tcagcaaaga taaatgaaaa atgttggtca tcatgaataa taaaatcaaa aaaatcgcca
    21241 ctgatctttt caaccggttt aaattttaaa taaaaagaaa atccttttat ttcagggaaa
    21301 gaaataggga ataaacactg ttgaaggtta cttgcaatat caagttcatt ttcaatctgt
    21361 actgaccttg cctgcaactg tgcatagagt ttttctaatt cattgttttt taatttcagc
    21421 tctcccacaa ggttaatatt ttcttttatg agtttataac gttcatatgc ccgttttaca
    21481 gccagtgaaa tctggtcatt tttaaacggt tttaaaataa aatcaaaagc tcctgccttg
    21541 agtgcagaaa tagctatttc aatatcatta taggctgtaa ttataatgat aggaatactt
    21601 tcatttattt catgtacctt ttgtataaat tccaatccat tgatgccggg taaattgaca
    21661 tcggtaacta tcacatcagc ctgcattatc acatcacttc gcaatgcatc ctcagcagta
    21721 taatagttat atactctata ttcatcatca agaataaatt ttaaaacttc attaaccatt
    21781 tcatcatcat caataatgac tacttcaggt ttttcaacag cattcattta gtcagcaccc
    21841 ttgtacagat aattcattga actgctataa aaacgaattc ccacccagtt tgcaggatgt
    21901 ttgtaccgtt tatcccgtat tacataaaca aatgcatctg cataagcagt aaatgatggt
    21961 ttacttttaa gagcagttaa aaattctcgt aaaaacaaca atcggtttac atcaataaca
    22021 gcgcaattga atgtaataaa tccacctatc cctttttgca tcaataccaa tcctgtatta
    22081 tatgattgcc cctgcatgat agtcaaatca attattaagc atcctggttt taccagagca
    22141 tcgtccataa caaaattttt tttattatca ctataccaca atggcttatc agtaattatt
    22201 atacttgatg agatggcaaa tccatagtga accccagatt gttttacagc agcaataatg
    22261 ctatcccggt tactatcgcc tatatttatt gcaataaccg aagggataac ccgcgtgcta
    22321 cccattgccg ctacaagaga aggcataaag gctaccacac ttcccgactg atggtacaga
    22381 attgcttcaa acggtattgc atcggtatat acatcggcaa agataataac acggctgtat
    22441 ggagcacatt cttttattac aggtttcatc ttttcatcaa tgttattttc aatcccccca
    22501 acaggctgca acaatccaag attgttatag tactcataaa ccagatcctt cacttcactg
    22561 taacaattgc tcaatcgtac tggctgaata taggtccgtg taataatcca cgcaaagata
    22621 tcattttcat tttttgctgt atataaaagt gctacatcct ttggcaattt tttttgaatc
    22681 gctgtgatgg ggattgcatt ccttaccagg ttgtcatact gttgcttaaa atattcatta
    22741 ttactcagta ctgctatgcc atatatcatc ctgtgtaatt cggcatattg caaggcatct
    22801 ttttgattgc ctctgctcat tgaataggat accatgtaat taaaaagttt atccagcaaa
    22861 tagggatagt cgctataata aaaacctgcc atatcactgc tgagcgaaac aatgaacctt
    22921 gcaatagcat tttcagcagc tttgaaaacg gattcatcgg tttttttggc tatagccatc
    22981 ttttgaagct gaatgttcat caaaaaaaga tcatacagcg atttatccca tgatatcatc
    23041 ccgctttcat caagcagttt ccctgcttct tttgttttcc ccatagcaat aacagaatac
    23101 aggtaacgca tataccactg acgttttatt tgaaaaggta gatcatggaa tgccgtgcac
    23161 agtgtatgcg cctgcttaaa ttttcccgtt ttttgatatg caacgatgtg ctgtaagaca
    23221 aaagtccatg ctggtttgcc tttttgtgaa gggatgcttt ctatgagttg tatggatcgt
    23281 tgcggcacac ttttattaca attatctgca atctttatgg tataaaactg tatgtcaacc
    23341 cacggagcat actgcaatgc atttttggta gccataagca tcttgagcca tgcctcttca
    23401 tttatatcag catgcaacac aatttcatgt attacaaaca aggacaacgc atcgtaatcc
    23461 atgctggtga gggtatcaac agagtattta ttattcttta atgagtttat gagcatacct
    23521 gcgttggtcg ctatagggta actatcgatt gcatagccaa gcgaatagac caaacaaagt
    23581 tcacgctgtg aaaggatata atccgctgta aactgttgta gcgcctttcc tgccttatca
    23641 tcctctttaa ggagagcagc aaattgtata tataataacc gtaattgatt gagtggtaac
    23701 agtgattttt catgattatc aaaggtatca tattctgcaa ggggttgatt gccattccat
    23761 gcttcaataa gcgcagcata cacctcatta tcaagagtcc cctgcaatgc cgcattccct
    23821 ttcaaagctt ttaaagcttc ctccatgtga ccctgtagca gatatgcata cacaaggtat
    23881 gaccgggcaa ttgaatcgtt atctgcaatc aactgtatta atgccactgc atcatctatt
    23941 tttccttgat agagcagcac cttgatctta tgtatagtat attcattatc atgcagtcct
    24001 gtttgtttcc accgctctaa tgccagtaaa gcctttgtgt aatccccctt accaagagca
    24061 ttattgaaca attgactgct ggattttgca acaaccagcg cattgctctc cctctgcttt
    24121 atatcagatg gagttttacc caatagcagt aatggtgtat tgacttcatg gagaaggtta
    24181 gcatcatcgc tgctgattgc tcttacaata gtttctaccc tgccaccagc ataatatcca
    24241 ataatagtag ccacatcagc atagagtgcc gcgtcactta aaagtaataa atcgttaagc
    24301 gatagtccct gaatattttt aacaacagca gaacactcta taacagtcgt aaaacaatac
    24361 cgggctgtta cagcatagag cttgttttca gaatcgacaa tatactctgc ttcatggatg
    24421 tcgttaataa catttggtat acctgacaat cgcatatccg ttgctatgga tgtcactggc
    24481 agccatactt ttttttctac ttcaggtgcc cgtgccgcac atggtataca tccaagctta
    24541 tacaacaggg tagtatcaag caatggatgt atagtcataa acgaatcatt taaaatgaca
    24601 tagccccggt taccatagtt ttgtaaaaca gcttcaatat cagcggttat agtttttgca
    24661 tttatttcat cagatattaa tctccaatca accgtatttt tatatgctat ccagcaatac
    24721 aactttccgt tatagtcata aaaatgcaac acaggcacat cataccgtgt cgctctataa
    24781 gcaccaacat agggtgccat gaaatctgta gcatttttac ggaatgtatc tatcttttta
    24841 tactgcatgg atagattttc ctttattgta tcaatgagtt ccttttttcc atcagctact
    24901 atcttctgcg ttaatgccct ttcaagctta taaacagtaa aacatgtttt tttgtaatga
    24961 gtaaaaaatt caacatcagc tttattgtaa aaatcatgga gcgttgctcc tgacattgct
    25021 aaagcaacaa tctgtttgcg ttgctgtgca gtaaatgcct ctctgtattt tcctgcattg
    25081 agcaagtaat ccgtatacct atcataaaga ttatagataa agcctgcata tcgcgcatat
    25141 aattgaggca acaactcaac acgcgataaa acaccagcta taaggctgtc aggattttca
    25201 atatgctgca ttaaactatt atagcgatta gcataatccc atacagtagc ttctgcaaac
    25261 cataccaatt cttttgactg tacagcgttt gccttgaatc gtgcgcttat aatatcatca
    25321 tagccttcct taaccttgcc ggtagcaata ttgcatatac ctctattgat aagtaatttt
    25381 atgacaaatt cctgatgata tggatcatta gcgttatagt caattgcctg tgataatagc
    25441 gctgatgctt gtgaatataa agaagctgtt gcagtgcttt gtcccaggga tgactggtta
    25501 ttggccataa ttgttgcttt ttgcatcagc aacagagcat aaagagcatt aaatgatatg
    25561 gtactgcctt caacctgttt tgttgcctgc tgcgataatt tttctatatc ctcagggaca
    25621 ggcctttttt tctgggcttt catagtattg atatagttat tgctaagttg agcataaaca
    25681 cgttcataca tgcttgttgt atagtgctct atggtgtcca acgctacatc agtttggtgc
    25741 agagcatcct ttgctccata actgcctgca gtataacagt aagcccattg cagcatcatc
    25801 cggtatacct tatcaacagc aatggattct ttctgagcta ttgccagcgc ttgttcatag
    25861 tagcgtacag catcagcaat ttgtccggat tgagcagcgc aatagccggc attttgcagt
    25921 gcagaaaatg taacatcaag gaacaggctg cctttgcgat tacctgctaa ctttataatt
    25981 ttatcatacc gtgtcagagc atcggtaaac ctgccattgc ttatataaat ctctgcagtc
    26041 atagaatgaa taagcaattg catttcaaaa gatgaaagct cagaaacaat ttttccttca
    26101 ccaatgataa tatcatcctg atttacagta aacacatata caggaccaag tgttactatt
    26161 tttaatccta ttttgtattg cttttctttt gttagtgata catttttttc agcaagttcc
    26221 agataagtta atgcttcttt atcgttcccc agtttacggt agcattcagc tattgctata
    26281 tataatcgtg agttactttc ctcatgatta tagcgtgccg catgtataac acatgtagta
    26341 taccagtcta tcgccttagt ataattcccc tgcatctgtt gaaataaggc aaaatattta
    26401 tataatgtat agagctgatc ttttactgaa agctcattct tctgagctat cgccttataa
    26461 atagtatatg cacgctgcat tgctgcatac gcaccgctat agtttccctg ttgccataaa
    26521 caatatccat aatggtaata aaactgtgcc tcttctttta atgaggtaaa tgctttttga
    26581 tagctgacag ccttgttata atgcggcagg gctaaagtat aatttttcag cagaaaatat
    26641 atatttgcca gattggtatg tatatccgcc tctttacggg gtgttataat ttcatcgttt
    26701 gcctccagag ctttctcgta cagtggaatg cattgttccc agaggtactc actaaaatat
    26761 gaatcaatca aacgcttctt agaagggcaa cacatccgca attcatcaac atactgatat
    26821 acccaccctt ttaatatata cgcatcaaca aacctatcat caataaacaa agcccagtca
    26881 atgtggtata gtgcctgttt caacatagca agctgtttaa ccaattcacc tgatgttctt
    26941 ccttccattg catggggtga cgagccaagt gtcagagctt cttgcacata gatatagcca
    27001 agtgcataga tatgcgcctt atcaaaatcc atacgcgcaa ccgaaagacg gtgaccctgc
    27061 ccggaatatt catctttaag agtatccagc atggtattct gtaatggaat actggcattg
    27121 atatatccaa tatgcgctcc tgcagcgttt atgttgtata catcctgata gcgacgcctt
    27181 agataaagca atgtattcat ggtaatgtag cgtttgtaca attcaccgga ctctttatat
    27241 tttccttcct catacagttt ttgtccgttt gtttcaaagt actctgtcag ttgctcaata
    27301 ataggacgga tatcctgttt ccactggacc tgaaagttat tagttgcagc ctcatagtag
    27361 ccagcccaat catcactctt ctgtttttgt gcaagctcac ctgattttac attgattaga
    27421 aaatatgcga catccaattt ttttgtattt gcaagcccct gttttaagta gagccatgcc
    27481 tcttcatttt gtcccagctc atcaaggctt gtggcagcta tgtattgaaa aattgccttt
    27541 gcaataggca gggtataccg ttcaacagct tccttagcac ctgcataccg ctgtgctgcc
    27601 ggtaattgtt ttgtagcatt aataacttcc atcgtaattt catttttaag ataggtgtac
    27661 gatgagccta acaccactat ccactcttca ggtatagtaa atcccccgta cagcaaccgg
    27721 ccaatcttaa agcgcacata cgcaagcctg gaataggtgg cataggtatc aacaatatca
    27781 cggtacgtcg ttaaagctct ctgtgtaaag ccctgagctg catacctatc agctaattct
    27841 tcaatgaaaa aaggcgttag tttttcattg ttttctaact tcaactcttg tatatttttt
    27901 tcaaggtatg ttattgtatt gttttctttt aatagtataa attgcgcata caatgatgcc
    27961 ggcttgcttt tgggcgatag ttcctgtaat atctgttttg caattgtata gtgttcatcc
    28021 cttgaaagct catacagggc ctttgcaata atcatctggg aatcaatatc caccgcctct
    28081 ctaaaaaaat aatatgttgc aaacaaacat cgctgatatc gttctatatc atcaaaatct
    28141 tctttatatt tcagtgccag ttcatactgt ctaagtgcat ttttttgttt tggtattatc
    28201 cccgtatggg gaatcattgc catgtttatg tgttcacctt caaccacact atacacaata
    28261 accccgtcag catatggcac ccatcgtggc gcagttgaat attcatcaat caatgttaac
    28321 ggatattctt cacccgttat tacattgtaa taatacagca taccgttatc actggcatca
    28381 accactccat cactgttagt atcatgctca acccgtgtaa aatagatatc atcatctgtt
    28441 tttccatagc acggtgatag ttgcagtgac gaattattgg tgagcttttg tatagcaccg
    28501 gttgcgatgg ttatccggta aatgttggtt tccttcccat cctgttttgt aaaaagaatc
    28561 gcattgccat catttgaaaa ccgtggttgc atgccactgg ttatcttgcg aactttatta
    28621 ccatccctgc tcataataaa aatactttcc tcaccttcgc gctcagatga aaaggcaatc
    28681 catttaccat caggcgaaaa gcatgggctg gcgtctcgta tggggactat agcgccggtt
    28741 gctgtatcct ttattgttgt aagattttgt gcaggttgct cgccttctaa tttgctctgt
    28801 gatagtatcg tgtctatact taatttcatg agcaaaatat caccctgggg gtcgtcccgt
    28861 gttgaaacaa atacacacca tttaccatca ggtgaaacat caggggcaaa atcttttgca
    28921 gggtgcactg ttaaacgaaa cgttgtgatg tcattcagtg gtctaagata tatatcaaaa
    28981 ttattgcgtt ctctgtttga tgcatagata aagtacttcc catcagtagt aatagtacca
    29041 cttacattta ccgaacgctc aaaggtaaga ggtataactc tgccagtacc aaaacgatac
    29101 tcttttgtta aaacctgtga ataatcaaat ggcacatcac gctgtgcagt tgaaacacat
    29161 ccagcaaacg atagcaagag taacagtact atgaacaata aacgcttcat ttcttcaccc
    29221 agcggccgtc attgttttgt atccagtcac cttttttagc ttttgcatgc tgctcttttg
    29281 caaattcaac tgcaaactgt atggcttttg cttcactgct tctggcagca ccagaacttt
    29341 taacaaagat tacactgcgg gcattatttt cctgttgtac aacggtgcgt attacatcag
    29401 cctcttgtag tgactgaagc tttttttcat caactattgc aatatatcca ttattctgtt
    29461 cgccaataag ctctttatcc ttgtataaac gtattgtatc gctgtttaat tctcttatct
    29521 ggatggcatt caacaactca tcatcagcat tgacaaccgc tttacgattc ccttttgatg
    29581 gggtcatcac cgttgatgta acccatgcgt cctgttcaag ctcatggtat tctccaatga
    29641 gctgtcgctc caatgcggtc ttttcaccgg taatattgat tgcagggggg tatatctcaa
    29701 ggcaacttgg catatcttga atgcagcctg aggaagtaca tccagacata acaataacta
    29761 tacaaacaat acacgcgagt gctatcgcca ccatgttttt tttcataatg attcctcccg
    29821 caccttgttg aaaaactcca tcaccggaat gcgttcatag cggacccgtt cattgttaac
    29881 cgtcaccagt gcacccagta ttttgcgctt aaataccacc gttggatata caagcccctt
    29941 atcaatataa aaatcaaagc cagcaatgat aagcgaatta tccacagcaa tttgtgccat
    30001 gcccaatttt gatttcccct tttcttcatt caagcctttg aacaatcggt tggcaaactg
    30061 ttcaccaacc ttgtaaatag tagcagtacc tgaaatgtca agattctgac cggtagataa
    30121 attctttccc tgcattatag cattcatgga taacaaagag tcgtgatgta ttcgcaggga
    30181 agatggatca tccagttttc caatatcaat atcattgaca ttaaacgcaa ctctaaatcc
    30241 catattctcc ggtttaagat ctgccagatt aaagagtaac tcctttaatg ttaccgttcc
    30301 ccccattact gttgcctgta cattctttat ttccagtgtg ttgcgttt
//
LOCUS       DAOSOX010000009        30280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1532_length_30280_cov_8.730223, whole genome shotgun sequence.
ACCESSION   DAOSOX010000009 DAOSOX010000000
VERSION     DAOSOX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30280
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1532_length_30280_cov_8.730223"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLX22_01360"
     CDS             <1..239
                     /locus_tag="PLX22_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18580.1"
                     /translation="KIRANASSKKDTSIDGLNLLEDTIKEKLPRFMDDVDRDERRDEE
                     IKALHRQEARVAEANKELRMLVLHLLIITKNLLR"
     gene            236..439
                     /locus_tag="PLX22_01365"
     CDS             236..439
                     /locus_tag="PLX22_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18581.1"
                     /translation="MNQEALIPDRWYDVDRRGVQKKGAFDTCHFTYCPETNWYQCPHG
                     IPLFFQNEFGQSPEIKRKGIVKR"
     gene            complement(607..855)
                     /locus_tag="PLX22_01370"
     CDS             complement(607..855)
                     /locus_tag="PLX22_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18582.1"
                     /translation="MATAGFIVGILSLFGTMLGCIPLLGALNWVNIPFGFLGIILSGI
                     AMASSNKQTKGKATAGLVMSIIAVIFGAIRLMMGGGIV"
     gene            complement(888..1439)
                     /locus_tag="PLX22_01375"
     CDS             complement(888..1439)
                     /locus_tag="PLX22_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18583.1"
                     /translation="MRSVKLIDVRQEESKTLNEFGRQKIINFLISKMGKNKFSQMVES
                     AKKNNQFDNEQWFSFVLSNLGSSIAFVLELNMCERCYDKSILYNAYYEVPVPDQSEEK
                     EAGTNGYSDATGILDIKGISNEDGNNPSNRNIVPVDIIKSFSKEEDVIKLKKGEVSPL
                     QGVLSRIEYEYEYGIRKITIRLK"
     gene            complement(1902..2036)
                     /locus_tag="PLX22_01380"
     CDS             complement(1902..2036)
                     /locus_tag="PLX22_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18584.1"
                     /translation="MNDLEIREAWIIAPVKETYMLTKTVRVAALYEFVSALAEGSSKS
                     "
     gene            2740..3588
                     /locus_tag="PLX22_01385"
     CDS             2740..3588
                     /locus_tag="PLX22_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18585.1"
                     /translation="MKKILLPLIITVFIHTNIYSYDLLKIGDNVTNYIKTNPVKYKLT
                     NQKLSNWPEGVVTLLAYDTKVDGKSPIIVFALDKYPIGVLQGTSPNAYYIFDIDGDGI
                     LDTKTDKGIVPFWVVYANSKNKSKNDNITSIFNLVYESFQSDEGPYINQKFKESINKL
                     YEFNKNINIENRDLAYLFLFYQLYNQYFPEQSLKCLDLLTKEYISRFNKIHPILLLYK
                     AETLINLKQEQDAKKIIAELRKADPNFIPAKAYEYRLEVDKEKAKILLNNLKENHKNH
                     WIVKQL"
     gene            3802..5220
                     /locus_tag="PLX22_01390"
     CDS             3802..5220
                     /locus_tag="PLX22_01390"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOT18586.1"
                     /translation="MKHVNFFLLIILVVVNLTGCSNDLFLIDIGKYGYIDKSGKIVIN
                     PQFEEAYDFSKEGLALVKLSDKYGYIDKSGKIVINPQFEKAYSFGEEGLAVVMLNKKY
                     GYIDKSGKIVINPQFEKAYDFSKEGLAAVKINQKWGYIDKSGKIVINPQFEDAYSFGK
                     EGLAAVKINQKWGYIDKSGKIVINPQFEDAYSFKEGLALVKLSDKYGYIDKSGKIVIN
                     PQFEDAYSFGEEGLARVKLSDKYGYIDKSGKIVINPQFEDAYSFGEEGLAIVKLSDKY
                     GYIDKSGKIVINPQFEDAYDFGEEGLALVKLSDKYGYIDKSGKIVIDTQFYYAEEFKN
                     GLALVGGEKYGYINKSGKYVWSYDKGADIYLGKDIVGWHITKKTDIFNSDDYFIMILD
                     NHYKFIGVTKIELRISVVKDAGVEQPFRIISYPIDSRAPMWGFNKKSITAGAFVGNRK
                     GKFELLIVGDNKTLASKIFEVR"
     gene            5445..5711
                     /locus_tag="PLX22_01395"
     CDS             5445..5711
                     /locus_tag="PLX22_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18587.1"
                     /translation="MLLPDPQTCINKINDFNHGITQQRTARDNCDYITLFQLIVNNIS
                     NNNSIPISCNLIRMLGLFQAYRNQPAQKNLNFISNLLNELGSSY"
     gene            5847..8435
                     /locus_tag="PLX22_01400"
     CDS             5847..8435
                     /locus_tag="PLX22_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT18588.1"
                     /translation="MDTINKASTGLPCLDKIVNNLIMGDNVVWQVDTIADYKTFVDPF
                     VSKALSEGRNLNYIRFAEFPPLLFEQPGIKIHYLNAHEGFESFSKRIYTIINREGENA
                     YYIFDSLSELQSAWATDLMIGNFFVITCPYLYELNAVAYFSLLRNSHSFKTIARIRDT
                     TQLLIDVYTCNGDCYIHPLKVQNRYSPTMFFPHKKNADYFTAITRSDDVTTFISHIQQ
                     TSLEKTQRNLDFWDRLFLKAEMLLELNEDPTKKQQMIEQLCKVIIGREERLLTLAANI
                     FTLEDLIAIKNRMIGTGHIGGKAAGMLIARRILHSDNRMQWNDIVEPHDSFYIGSDVF
                     YTYIVQNGWWKLFMEQKTQEGYFTVAKEIAQQMLHGIFTDDIKEQFQQMIEYFGQSPI
                     IVRSSSLLEDSFGNAFAGKYESIFLVNQGSPAQRYQAFIDAVRQIYSSTMNEDALVYR
                     LQRGLHTMNEQMAPLVQRVSGTHRGNYFFPDISGVGFSHNTYVWKKEMKPESGMIRLV
                     IGLGTRAVNRVEGDYPRIVALDDPRSKPLYDFKDVRRFSQHNVDVLNITTNQVETIQM
                     DDLFNIQDLNLPMHLLGIRDYEAEQTIKNMNGEQKEYWVVTFDELLMHTDFTSNIKTI
                     LQTIHNRYQYPVDIEFTVNFNKEEGYYINILQCRPNQIKGLGKKVKLPEDIPGDHIFF
                     TSRGNFLGGSIVQPIKRIIYVDPYLYNSLSISQKYDVARTVGKLNKAIQDREEMPALL
                     IGPGRWGTTTPSLGVPVRFAEINNIKVLVELALINENIMPELSFGTHFFQDLVETDIF
                     YVALFYGISNVLYHQNFLEQFPDIFTKLLPDSQYNHVIQVFDLKEHDVELMADITTQK
                     VLCFMS"
     gene            8675..10033
                     /gene="gdhA"
                     /locus_tag="PLX22_01405"
     CDS             8675..10033
                     /gene="gdhA"
                     /locus_tag="PLX22_01405"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOT18589.1"
                     /translation="MSVISEVIDKVKRQDSEQKEFIQAVHEVLETLEPTVQKHPEFVK
                     AKIYERIVEPDRVIIFRVPWVDDNGNVHVNRGFRVQFNNAIGPYKGGLRFHPSVNLGI
                     IKFLGFEQIFKNSLTTLPMGGAKGGSDFDPKGKSDMDVMRFCQSFMRELYRHIGPDTD
                     VPAGDIGVGAREIGFMFGYYKKIRNDHTGVLTGKGLEYGGSLLRPEATGYGATYFAAE
                     MLKTVGKEIKGMTVAVSGSGNVAQYTIEKVTQLGGKVISCSDSNGTIIDEEGITPDKL
                     AFLMELKNVRRGRVKEYAEKYKNAHYFEGKSVWDVIKEQGIKISVAFPSATENEIDGK
                     HAEALIKNGCICVSEGANMPSNLDAIKVYREHNILFAPGKAANAGGVATSGLEMSQNS
                     LRLSWTREEVDNRLQIIMKSIHKSCLDASEAYGKKGDYLTGANIAGFLKVAKAMVAYG
                     IV"
     gene            complement(10103..12013)
                     /locus_tag="PLX22_01410"
     CDS             complement(10103..12013)
                     /locus_tag="PLX22_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT18590.1"
                     /translation="MGELKTMVRYFYDTCDRLPDRPAQKFNKELYDDNNGLFTYAELK
                     ERIEDIACALMKLGFPYKGRAAILAPTSYMWTQVDIAIACAGGASITIFPTLSNNEVK
                     YIMNDSRCTIVFVGDKTLLKQVKELEKDLKSLKTIIVMDMSFKGDKKKVFGIKDCIAM
                     GREWRNNQANYKKYNKRKDSVTLDDWFTILYTSGTTGQGKGVILTHWCGSSRLEGTTE
                     YFQRYNFDVNENDVTLCYLPLSHVFDRGSCQWLAISKGSLICYADKPATLIEDMQKYN
                     PTWINCVPRLYEKIYITMQQQMAENPLQKKLFNWALNVGLQALKYREDENGCYNMHPN
                     FDLKSRLPLWLKIQYSIADKIFAKVRGLFGKRFHHSFSASAAISPDLLRFYYALGLAV
                     IEGYGSTESFNAAILNPITACKPGYMGKEANGSKCRVSKDGELELSGAGIFKGYLNKP
                     KETKETFTKDGWFKTGDIVELSSDGYFKFIERKKGIICTSIGKNVAPAKIENLFSTSN
                     WVEQIFLVGDEKPFITALIVPNFANFMALFKREGITYDESKLVYEDVGGMQLCVQVGD
                     DFINNYRLKELIDNDVKEANKHLEKFEQIKNYTILNQRFTESNGMLTPTQKTKKRVIV
                     EVFKDEINKMYA"
     gene            complement(12106..14742)
                     /gene="alaS"
                     /locus_tag="PLX22_01415"
     CDS             complement(12106..14742)
                     /gene="alaS"
                     /locus_tag="PLX22_01415"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT18591.1"
                     /translation="MMKVHDLRVAFIEYFKQRDHRIVPSSSLIPQDDPTLLFTTAGMV
                     QFKPMFAGTINAEYTRAASIQKCLRTSDLANVGKTKRHCTFFEMLGNFSFGDYFKKEA
                     IQWAWEFSTQVIQFPEENIWVSIYTDDDEAFDIWHTIIGIPEQKIVRLGKEDNFWGPA
                     GDSGACGPCSELYLDRGASFGCGKPDCKPGCDCERFLEFWNLVFNQYFQDTEGKLHPL
                     PKTGIDTGMGLERLATLVQNVDSIYQTDELNELVNYVINKRNIRYEGDAQVAVNVLVE
                     HARTLTFAISDGAYPSNEGRGYVLRRILRRALRYSRQIGIHEPFVYTMVEPIVTIMGK
                     QYPELSSTKENVKAVIKGEEERFLETIENGMDRLDEIVNALKKKGEKTIQGNDVFVLY
                     DTFGFPVEMTEEIAQEHGMTIDKVGFEKKMEEQRTRGRQSWKGAEGSIDTIVGQLAKI
                     IQRTEFEGYEKYDSESDVVAMSNFEDLKVQLADGERGIIVVKNTPFYGESGGQVGDTG
                     YIYGDNGTFRVEDTKKSGDLIIHVGQMEKGYLQEGEHVRLSIDGIRRNCIRANHTATH
                     LLQAALRNVLGQHVKQAGSLVEPERFRFDFTHFKAMSHDELLQVETMVNEKIWACLPV
                     ETSVMPLQDAIHAGAMAVFGEKYSDIVRMVSVNNFSKELCGGTHVDNTGKIGVFRIMH
                     EASPGAGVRRIEGVTLKGAYERLLHDQHLLGRVMRLLEVNESNESNVEKKITEMVEKV
                     SLLEKELEKTKEKGLKSIAKDLYDKAETINGIKFVSAVLQNMDAEALRSVADFVRELS
                     VAVVVCLASSHDEKAFIIAAANKIAVDKGIDCNAIIKAVAKKIGGGGGGRKDLAQAGG
                     KSPENVGIALSMCKEILLNSFK"
     gene            complement(15014..15655)
                     /locus_tag="PLX22_01420"
     CDS             complement(15014..15655)
                     /locus_tag="PLX22_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOT18592.1"
                     /translation="MVIIDIEYSDRYIMITLDSGEHLKIDSVSVPQGLLRKGTEIDID
                     AYEHIKEESKLFECKEKALYYIGLRDRSEHEITKYLIKKGFDKNTIYRVIKLFIEKGF
                     IDDYHYALQYVRSTHEHKVVGNRVIEKKLFERGIHKSLIKKAIKKMQDNEPDIERIYQ
                     IAQKKYNRVKGKEHAREKVGLFLQQRGFSWDTIKEVLNRLGDNNSDNNNFIDD"
     gene            complement(15742..16134)
                     /gene="rpsI"
                     /locus_tag="PLX22_01425"
     CDS             complement(15742..16134)
                     /gene="rpsI"
                     /locus_tag="PLX22_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT18593.1"
                     /translation="MANAVNYATGRRKSAVARVWLKPGNGAIVINSKPINEYFRRQTL
                     ELVVRQPLEEAGVLASFDVYATVNGGGWTGQAGAVKLGIARCLSALDEKYKKSMRQAG
                     FLTRDSREVERKKYGQKKARKRFQFSKR"
     gene            complement(16177..16623)
                     /gene="rplM"
                     /locus_tag="PLX22_01430"
     CDS             complement(16177..16623)
                     /gene="rplM"
                     /locus_tag="PLX22_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT18594.1"
                     /translation="MSIWQRTFSLKKSQIEKKWYIIDAEGKVLGRLAAEIAHVLRGKH
                     KPTYSAHLDMGDNIVVINAEKILLTGKKPDDKEYFFHSGYPGGMKFVNIKKMLQKKPE
                     YVLYHAVKGMMPKNKLSRNIMGNLKIYAGKDHPHQAQKPENLELKF"
     gene            complement(16791..16946)
                     /locus_tag="PLX22_01435"
     CDS             complement(16791..16946)
                     /locus_tag="PLX22_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18595.1"
                     /translation="MTINKQVQDKIMKATVIVNPSSRSGLKVYQTLTHYMDNYYYHED
                     QIFILIP"
     gene            complement(17027..18064)
                     /locus_tag="PLX22_01440"
     CDS             complement(17027..18064)
                     /locus_tag="PLX22_01440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT18596.1"
                     /translation="MMKTRITQLFGIKYPIILPGMSWISTPELVAAVCNAGGTGYLAT
                     GPLTAEQTRAAIQRIRELTNKPFGAGCTLIMPGARENAEVMLEEKVPIINVSLGKCDW
                     IAERAHKYGGKVIATVVTEKHALAAEKQGADALQVTGHEAAAHGGQVTTFVLVPAIVD
                     KVKIPVVAVGGIADGRGMAAALALGAEGIGMGTRLSMTKESPLHKKTIEAQLKAGIED
                     TIYSNRFDGIYCRVLKTKSAEKAIKKGMSLPHAAIQSYKIAKMMNLPYFKLVLGVMLQ
                     GPKMMIQLAHFATAFDKIKVATEDGNLEYGVQLIGQCQGLINDVPAVKDVIERTVKEA
                     KAIYQKNLKKF"
     gene            complement(18182..>18876)
                     /locus_tag="PLX22_01445"
     CDS             complement(18182..>18876)
                     /locus_tag="PLX22_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT18597.1"
                     /translation="AQGYGLTEFFPVAVGTDKTAYPGICGPVLDIVDVRVSDEGEIQL
                     KGPMCMDGYYNKPEATREMFTSDGWFKTGDVGTVEKHNDNLYIRITDRIKDLIITAGG
                     KNIAPQVIETMLGEDLYIEQVVVVGDGRKYISALIVPNFEMLHEYAKSKGIQYSSRQE
                     LISHPGILEFYNNKIEKLTEGLGQVEKVKRFTLMPAEFTQDNGEITPTMKIRRKVIQQ
                     RYSDVINKMYED"
     assembly_gap    18877..18976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18977..19812)
                     /locus_tag="PLX22_01450"
     CDS             complement(<18977..19812)
                     /locus_tag="PLX22_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT18598.1"
                     /translation="MKSTTELSSKNIIAKGFVMQKATLQGLHDFLSEEKTVVSMLLSR
                     ADARGDSIALTGFKGDEKYSYTWSELKHHAYAIGYFLISHGCQSGDKIAIFAQNCPEW
                     TIVDLGIQSSGCVPVPIYATNSKDEARYIIEDAGIKVVFTGDQEQFDKILEIATMSSV
                     THVISLQDDVTVTSEKGFYVKDIINTTVDSAVIEQVTSRMRGLSSNDMLTIIYTSGTT
                     GNPKGAVHTHASFLAGIYASVVRFPEAGPGMVSLAFLPLSHVFERMWSYGVLLKGGEN
                     HY"
     gene            complement(19824..20909)
                     /locus_tag="PLX22_01455"
     CDS             complement(19824..20909)
                     /locus_tag="PLX22_01455"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT18599.1"
                     /translation="MEKRHTYSNLYIKEYSMKTRITELFKIQYPIVLSGMSWISTPEL
                     VAAVSNAGGLGILATGVLTADQTREAVQKIRSLTKKPFGANVTLYFPGAERNAQVLIE
                     EKVPIINYSLGKGDKIAKAAHAYGGKVVATVTTHKHAFAAQRDGADALIVTGYEAAGH
                     GGAITSLVLIPAIADALDIPIIAAGGFADGRGLLAALALGADGIAMGTRFMNTKESPA
                     AEQAKQASVKSEVYDTIYTPKVDGLPARFMKSKGVEHLLKKRLNPFSALIRSKKIADM
                     LEFPWLKLAIGIMLMGPKRSMQMAQMAIGFDAFKLGTIDGDFAKGVLPLGQVSGIIKD
                     TPTVKQVVDSIVKEAVAIEKQLSKKVK"
     gene            complement(20989..22311)
                     /locus_tag="PLX22_01460"
     CDS             complement(20989..22311)
                     /locus_tag="PLX22_01460"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT18600.1"
                     /translation="MLEAAKRNHDAIRKECQGVQCRWWDIYSNDTISKSVTFKNNVFH
                     SARESAVRGYGIRLNIHGKTGFSYCNDFNDIKTVIAHAKDTAQYGDNEDYALPGKNAI
                     PKVTLYDEQWLESWTVENQVQQVQAIIDTIRKAYSDCMVDSSAGCVDGYVYLTNSSGF
                     EAGYRYLQHGVSVSGLRIVSDGSRMQVYESTSWGSTFDYHEIVNRLIMKLQFSETNAA
                     IPSGNYYAILTPKAFGQIMGVVLQGLSGKSIQRGISRYIGKFGQKVFGKQLSVYDNPL
                     EHFAPGSYPFDGEGVMAFNKTIIECGYVQRHIAHLQSSHILQCNPTGNASRSYATLPQ
                     ESFSNIVVPGGDVSFKDMIRQMDTGIIIDQFIGFGQSNTLMGQFSANLDMAWFVNNGE
                     VQGRLKNCMVADDVTRMLDDTIVLSKEREWIGDSYLPYVMCRIDYTTI"
     gene            complement(22283..23680)
                     /locus_tag="PLX22_01465"
     CDS             complement(22283..23680)
                     /locus_tag="PLX22_01465"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT18601.1"
                     /translation="MVVDMTMAQKKLKNIKAEYAEIRMSESDTVSIHLSKNRVETCKA
                     GKTTQGSVRVYNRGCWGFVSFTTLDMIDYAIIRAQELSHIQKKDDAVSIAAMQSNVVN
                     KKTKVLKPLHSVSLEEKMELMHRYNAILKSSPLVENTNVVYRDSMTHYYMYNTDETGV
                     DYESSFCGVSFSAIAKDGTVMQPYGESIAQYGGFELVEDNHAVAEEVVKTAAELVKAE
                     QVQSGLYNVIVDPKLAGVFIHEAFGHLSEADFIYENPRMKELMVIGRRFGPECLQVID
                     DGSLPYTGYIPIDDEGIQPQKTYLIKDGILTGRLHSRETAKKMGEQPTGNARAISCGA
                     HPIVRMTNTYIDNGTATADELFDRLGNGLYVAGALGGQTNLEMFTFTPAYAYEVKRGK
                     KTRLLRDCMLSGNVFMTLMNIDSIADDCTMFGGLGGCGKQGQAPLPVSSGGPHLLIKN
                     VLIGGSGAGGSKKKS"
     gene            complement(23799..24500)
                     /locus_tag="PLX22_01470"
     CDS             complement(23799..24500)
                     /locus_tag="PLX22_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOT18602.1"
                     /translation="MAKNTCIVTCEHAGNYIPPELTALFSGEHEIVATHLAYDIGALP
                     MAMIIASILHVPLIYTKVTRLIVDCNRSVGSDELFSKFTKNFDSVLKNELIRRYYDKH
                     RNTIEQAVSRAMRHHTHVIHVAVHSFTPQLNGFKRYCDIGLLYDQYCPVEQQLSKSIK
                     ETLFALHPLRIAYNYPFRGCGDGVTVWLRKQYGDRYCGIEVEVNQDLYFQGNHLWRQL
                     SSNIGQAIKRAMTTV"
     gene            24589..24945
                     /locus_tag="PLX22_01475"
     CDS             24589..24945
                     /locus_tag="PLX22_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT18603.1"
                     /translation="MLIITTIIGLSAGMLSGLMGIGGGIIMIPALIYVMHFNQHLAQG
                     TTLAAMIPPIGILAAYAYYKSGYVNIPVALTLALGFIIGGYFGGKIAVSLDSETLRRI
                     FGIILFVISLHIIFSK"
     gene            25121..25402
                     /locus_tag="PLX22_01480"
     CDS             25121..25402
                     /locus_tag="PLX22_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18604.1"
                     /translation="MQQQNSGIQKPQRIKPFYSQKVPIPSNLTSLLWDEQDTAPLEKL
                     ILRVLQYGNYEEIHTIYSLYSTETTDIVSRYPDIKRGVKFWVRYWNEHH"
     gene            25389..25964
                     /locus_tag="PLX22_01485"
     CDS             25389..25964
                     /locus_tag="PLX22_01485"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT18605.1"
                     /translation="MNTINTTIITLAQQIQEQFPHFYLAGGTAIMLKYNHRISYDLDF
                     FNFKEFSYNRLAKKVIPLYQNQIDKWERKEDNIDFFINGIKVSFVFFPFKNLEKIETI
                     HHIKVAGDFDLFLNKLYVAGRRIDPKDPFDAAYLYNQHNWDNNYIIQGFEKKFKGQSY
                     KIYLGALLSFEDYGELPAWIKETLMKLIYQV"
     gene            complement(25948..28293)
                     /locus_tag="PLX22_01490"
     CDS             complement(25948..28293)
                     /locus_tag="PLX22_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT18606.1"
                     /translation="MNKKVLLAIKNNNREAITKLITSTPGISGVNYNNKHYEIEYDSS
                     VISPIHIVELLEENGVYIEKEIVQATIGDMSCVMCSKAVTNAIMDIEGVLDINVNYAT
                     GKAMLVVTPEAVHDSDIKTKVEDAGYRFLGTEEVQTQKQSPLRLIQIVAGLVAGALLM
                     AMMYGLWAMDTIGIAIILTPVFLFVSYHIFTGAYHALKNRVLSMDVMYAMGIGVSFGA
                     SILASLHVLSHEFMIYETSIFLASFLNIGKYLEERAKSRTSHTIAKLLSLSPDTAIVI
                     RDGNEIEINTSDVTIGDTVVVKPQTRIPVDGEIVKGQSYVDESMVTGESVPVFKSEGN
                     AVIGGTLNKNSLIVIKAKAIGQDSILSRIINLVEHAVMSKPRVQRFADRVVSIFIPVI
                     LTIATIAALVWYVINGDIFFSFEVFVAVIVIACPCALGLATPTAITVGIGKGAELGIL
                     IKNSEALENASKVTHAVFDKTGTLTTGTMTVTNIIAFDNISEHELLSVCASLERYAEH
                     PVGEAIVQYATNHHAVFHEVEDVSVVEGKGVMGTIAQKKVIAGSRDFIQDYIPCEVTI
                     AQKMKEFENEGKTVVVVAQEKPYGIIALSDTPRTDANTVIRSLQQRGIKTAMLTGDNR
                     LSALAIAHEIGMDAVYADVKPEDKMNVVKQLQKDGEIVAFIGDGINDAPVLAQADVGI
                     ALGSGTDVAIEAGDVVLLRNRLRDVVTYFELAVKVMRRIKQNIFWAFAYNAVLVPVAA
                     GVLYPFTGWLVKPELAGAAMALSSVTVVSLSLMLKRFKPGI"
     gene            complement(28494..>30280)
                     /locus_tag="PLX22_01495"
     CDS             complement(28494..>30280)
                     /locus_tag="PLX22_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18607.1"
                     /translation="VKVNKSFWETSPAIYVPGANTAYELYFNGNKFMCSGIVSIDEQS
                     SSPCLKPQLKPLNCEKETEEFVIAVKVSNFFHRGGGLNKCIVIGPFESITQILWEHDF
                     IKTVILGIIFIIAVYHLILWMGRRKDKTSFWFSIFCFAIFSHSFVIGNYLERIFYNSD
                     VFFIRFSIEYGSLGLGWLSFTLYVCYLFPRESNRMFVIFQIITGIIFISIPMILPPKI
                     FTNLIGAYNVILFTTMIGAFIILIAASIRRRDDALLIFIGYIVLMITVANDILHNLFI
                     IRTFFMSEYGLALFILFQSSVLSLRFARAFKTTEYLSENLNAEVKKKTEELLNQTEKA
                     ISAQQDTEKTYKKLASLYDQISNEVHLAKTIHDRIIPKEIMHIPDVKIAVYNEPFMQI
                     GGDVYDIASLPNKKTRVFLADATGHGISASLITILIKVEYDKLKDECETPAEVFKKLN
                     TVFSDYYKTMKMFLTAIMVDIDGNNNKLLYASAGHPDQFLVMNSTIFELSRTGPAIGI
                     SKFSNFENKIIDIKKNYKLYLFTDGIIEEFSTDYFGNQYKDLKHFILENGHLSVEQTV
                     SLINEAIKRNASNIEHRDDKIFIGIESI"
BASE COUNT         9251 a         6358 c         4930 g         9641 t
ORIGIN      
        1 tcaagatacg ggcgaatgcg tcaagcaaga aggacacgag cattgatggt ttgaacctgc
       61 tggaagacac cattaaggag aaactaccga gattcatgga tgatgtggac cgagatgagc
      121 gcagggatga agaaattaag gcgttgcatc gtcaggaggc acgggtagcc gaggcaaaca
      181 aggaactgcg gatgctggtt ttgcatctct tgataattac gaaaaacttg ctgcgatgaa
      241 tcaggaagca ttgatacctg acagatggta tgatgttgac aggcgtggtg ttcagaagaa
      301 aggagcgttt gatacatgtc atttcaccta ttgtcctgaa acaaactggt atcaatgtcc
      361 ccatggtata ccactttttt ttcagaatga attcggacaa tcacccgaga taaaaaggaa
      421 gggtatcgtg aagcgatgag gcttgttttt taagcgatgc tatctggtag aaagtccatt
      481 tgttatatag cagtaagggg aagtcaatag tttaaaagtt aaaaattcga tagcctgatt
      541 aaataaattt tatcaacccc tatagccaaa ttattatgga ataataatca atatatagac
      601 ttaattctat acaattcccc cacccatcat caatcgtatt gctccaaaaa ttactgcaat
      661 tattgacatt acaagacctg cggtagcttt cccctttgtt tgcttattac tgcttgccat
      721 ggcaatgcca gaaagaatga tacctaaaaa accaaaagga atattcaccc aattcaacgc
      781 ccctaaaagg ggaatacatc caagcattgt accaaacaac gaaagaattc ccacaataaa
      841 tcctgctgta gccattagct aaccctccta aaaaaatgat tttaaactta ttttaacctt
      901 atggtaattt tcctgatacc atattcatat tcatactcga ttcttgataa aactccctga
      961 agaggtgaaa cctcgccttt tttcaatttt atcacatcct cttcttttga aaagctctta
     1021 attatatcaa caggcactat attacgatta cttggattat ttccatcttc attgcttata
     1081 cctttaatat caagaatgcc ggtggcatct gaatatccgt tagtgccggc ctctttttcc
     1141 tcgctctgat caggcaccgg tacctcgtaa taagcattat acagtattga tttgtcataa
     1201 caacgctcgc acatatttaa ttccagtacg aacgctattg acgaaccgag attgctgagc
     1261 acgaaagaaa accactgttc attatcaaac tggttattct tcttagcgga ctcaaccatc
     1321 tgcgaaaatt tatttttccc catcttggaa atgagaaaat taatgatttt ctgccgccca
     1381 aattcattca gcgtcttaga ctcttcttgc cttacatcaa tcaattttac agatcgcatg
     1441 cagaactgtt tacccctata cgcagcgttt attctataca gttctttcgc atactgcttt
     1501 tccttgtatt ccctttgtac ctccgtatca gattcatcat aaatatactt ttcgactcca
     1561 tttaacagcg accatatgta tctggaatca gattcccagt ctgatatata agtactcctc
     1621 tgctgaatat tttcattggc atgcagtgaa aagccaaatg gaaggagaga taaaatggca
     1681 atttggcaaa tatttctaat taaaccgaat ttcattcata tattatttca tagaattgct
     1741 attttgtcaa caactaaatt aatcaaatat ttatatcaga agatataaaa aatagccgtt
     1801 taccacatgg aaaaggcagc cgggaaaccg cctttttgtt ggacgaaatt ttgctggtca
     1861 tccgcatatc gttctacaaa atcacattcc gcttgcaccc gttagctttt gctgcttccc
     1921 tccgcgagcg ctgaaacaaa ttcatacagc gccgcgacgc ggaccgtttt cgtgagcatg
     1981 taggtttcct tcacgggagc gatgatccac gcttcccgta tttcaagatc gttcatggcg
     2041 attgccagtc cttttcctgt ttctggtgat gccgacgctt tgcactacac cgcgaccagt
     2101 cttctccccc tgcccagcac catgtctatc tcggttccgg atgatgtccg atagaataca
     2161 gcctcccggc cgggcagcga gctgatcaca ttttccacca caagccccca ggaagcgccg
     2221 aacaccggat accatacagc cattaatacg ggtttactgc tggacattgt tctcctcggg
     2281 gagcagtccc tcggatttcg gtatcgggga aagttattct cgatgactca tcggcggttc
     2341 actttcattt atctccttta gattcaccgg gtctatttgc atggatcgtt ttgccccttt
     2401 ggttcagtac caccgggact acccccgaca gcaccaaagg gggggtttag agactgtttc
     2461 cggaaaaccc tctctggcag ttcatcgttc ccttgactct gccattatgc ataaagattg
     2521 cgctgaatct tcttgacatt cttcctacta actgcttcac tctatatagg caaccctgat
     2581 tcagcttcag ggcacacgac ttcatataac taagcaaagc cgctgcggtg ctcggcctcc
     2641 gcgcctatga tatgaagacg gccgctccgg caaaaacttc agctatgctg agacgttata
     2701 cgacattttt acaattttta actaaaagga ggaaaccaca tgaaaaaaat tctattacct
     2761 ttaattatta ctgtttttat tcataccaat atttactctt atgatttatt aaaaattggt
     2821 gataatgtaa caaattatat taaaactaat ccggttaaat ataaactgac aaatcaaaaa
     2881 ttatcaaact ggccagaagg tgttgtaaca ctattagctt atgacacaaa agttgatggc
     2941 aaatcaccaa taatagtttt tgcattagat aaatatccca ttggtgtttt acagggtaca
     3001 agtccaaatg cctattatat atttgatatt gatggagatg gtatactaga cacaaaaaca
     3061 gataagggaa ttgttccatt ttgggttgtc tatgctaatt caaaaaataa atcaaaaaat
     3121 gataatatta catctatttt taatttagta tatgaaagtt ttcaatctga tgaaggacca
     3181 tatataaatc agaaattcaa agaatcaatt aacaaattgt acgaatttaa taaaaacata
     3241 aatatagaaa atcgagactt agcatattta tttttgtttt accaattata taatcaatat
     3301 tttcctgaac agtcattaaa gtgtttagat cttttaacaa aagaatacat atctagattt
     3361 aataaaattc atcccatttt attattatat aaagctgaaa cattaattaa tcttaagcaa
     3421 gagcaagatg caaagaaaat tattgcagag ttaagaaaag cagatccaaa tttcatacct
     3481 gcaaaagctt atgaatatag actagaagtt gataaagaaa aagccaaaat attattaaat
     3541 aatttaaagg aaaaccataa aaatcactgg atagtgaaac aattgtaaaa acggcgtata
     3601 acacagcata tgcgtaattg ccctaaattc aaaacataaa atatttattg acataaagat
     3661 caatctatta aataaattta acgtgttcca tgctactatg cattatactg gtaacattat
     3721 atgcaatggc aacataactt tggccgggcg tccgtgcccg ccttgtgggg aataaaattt
     3781 aacctgccgg aggagaatac catgaaacac gtaaattttt ttcttttaat tattttggtt
     3841 gtcgtaaatc ttaccggttg tagtaatgat ttatttctta ttgatattgg taaatatggg
     3901 tatattgata aatctggtaa aattgtaatc aatcctcaat ttgaagaagc ttatgatttt
     3961 agcaaggaag gtcttgcttt agtcaaacta tctgataaat atgggtatat tgataaatct
     4021 ggtaaaatcg taatcaatcc tcaatttgaa aaagcttata gttttggtga agaagggctt
     4081 gctgtagtta tgctgaataa aaaatatgga tatattgata aatctggtaa aattgtaatc
     4141 aatcctcaat ttgaaaaagc ttatgatttt agcaaggaag ggcttgcagc agttaaaata
     4201 aaccaaaaat ggggatatat tgataaatcg ggtaaaattg taatcaatcc tcaatttgaa
     4261 gatgcttata gttttggtaa agaagggctt gcagcagtta aaataaacca aaaatgggga
     4321 tatattgata aatcgggtaa aattgtaatc aatcctcaat ttgaagatgc ttatagtttt
     4381 aaggaaggtc ttgctttagt caaactatct gataaatatg ggtatattga taaatctggt
     4441 aaaatcgtaa tcaatcctca atttgaagat gcttatagtt ttggtgaaga agggcttgct
     4501 agagtcaaac tatctgataa atatggatat attgataaat ctggtaaaat cgtaatcaat
     4561 cctcaatttg aagatgctta tagttttggt gaagaagggc ttgctatagt caaactatct
     4621 gataaatatg gatatattga taaatctggt aaaattgtaa tcaatcctca atttgaagat
     4681 gcttatgatt ttggtgaaga agggcttgct ctagtcaaat tatctgataa atatggatat
     4741 attgataaat ctggtaaaat tgtaatagat acacagtttt attatgcaga agaatttaaa
     4801 aatggtttag cactggtagg tggtgaaaaa tatggttata tcaacaaatc tggaaaatat
     4861 gtatggtcat atgataaagg agcagatatt tatttaggca aagacattgt tggttggcac
     4921 ataactaaaa aaacagatat ttttaatagc gatgattatt ttataatgat actagataat
     4981 cattataagt ttattggtgt tacaaaaata gaattacgaa taagtgttgt aaaagatgct
     5041 ggtgttgaac aaccattccg tataatttca tatccaattg attcaagagc ccctatgtgg
     5101 gggtttaata aaaaaagtat tacagctgga gcttttgtcg gtaatagaaa aggtaaattt
     5161 gaattattga tagttggaga taataaaacc ttagcttcta aaatttttga ggtcagataa
     5221 aatattaaat ttattacttg cttttcgccg tcatggctca aagcaaaaaa gtcttgatgt
     5281 tttgccactg cgtacaacaa agcttatgtg ctgcgcttac tgtcgttcgc ttgggcttcg
     5341 ccacatttta accttcgcta tcgctacggc aaaaacgtcg catacgccgg taacgttggg
     5401 catgattttt gaataatttc attgaactcg gggaggttat atctatgcta cttcctgatc
     5461 cacaaacatg tattaataaa attaatgatt tcaaccatgg aatcactcaa caaagaactg
     5521 ctcgtgataa ttgtgattat ataacactat tccaattgat tgtaaataat atttctaata
     5581 ataattcaat accaatttca tgcaatttaa ttagaatgct tggtttattt caagcctata
     5641 gaaatcagcc agcccaaaaa aatttaaatt ttattagtaa tttattaaat gaattaggtt
     5701 catcatacta aataatcgat actatttact ttaccactga ctattttcac aattacagtc
     5761 ctgctacagc tacattatat agaatagttt acaaatgaca tatatattat actataataa
     5821 tcatccttac tatcaggaga tctataatgg atactataaa taaagcaagc accggactcc
     5881 catgcttaga caaaattgta aacaacctca ttatgggtga taatgtagtg tggcaggttg
     5941 ataccattgc tgattataaa acatttgttg atccttttgt ttcaaaggca ctttcagaag
     6001 gtagaaattt gaattatatc cgctttgcag agtttcctcc cctcctcttt gaacagccgg
     6061 gaataaaaat acattatctc aatgcacatg agggctttga aagcttttca aaacgaattt
     6121 ataccattat taacagggaa ggtgagaatg catattatat atttgatagt ctttctgaac
     6181 ttcaatcagc atgggcaacc gatctcatga tagggaattt ctttgtcatt acatgcccat
     6241 atctttatga attaaacgct gttgcatatt tttctttatt gcgcaatagc cactcattta
     6301 aaacaatagc tcgtattcgc gataccacac aattactcat tgatgtatac acatgcaatg
     6361 gcgattgcta catacatccc ctgaaggttc aaaatcgcta ttccccaacc atgttcttcc
     6421 ctcacaaaaa aaatgcagac tattttactg caattacccg aagtgatgat gtcaccacat
     6481 tcatatccca tatacagcaa acaagcttag aaaaaacaca aaggaacctt gatttctggg
     6541 ataggctttt tcttaaagct gaaatgttac tagaattaaa cgaggacccc accaaaaaac
     6601 aacagatgat tgaacagcta tgcaaggtaa taattggacg ggaggagcgt ttacttacct
     6661 tagccgccaa tatctttacc ctggaggacc ttattgcaat caaaaacaga atgattggga
     6721 caggtcatat aggtggaaaa gcagcgggta tgctcattgc ccgccgtatt ttacatagtg
     6781 ataacagaat gcaatggaat gatattgtag aaccccatga ctcattttac ataggttcag
     6841 atgttttcta tacctatatt gttcaaaacg ggtggtggaa gttattcatg gaacaaaaaa
     6901 ctcaagaagg atacttcact gtagcaaaag aaattgctca acaaatgttg catggcattt
     6961 ttaccgatga catcaaggag caatttcagc agatgattga atattttgga caatctccca
     7021 ttattgtccg ttcatcaagc cttctggaag attcttttgg taatgcattt gctggaaaat
     7081 atgaaagtat atttcttgtt aatcagggtt ctcccgctca acggtatcaa gcttttattg
     7141 atgcagtacg tcaaatttat tccagcacca tgaatgaaga tgcacttgtg taccggctcc
     7201 agcgagggct tcatacaatg aacgaacaga tggcacctct ggtacaacgg gtatccggca
     7261 ctcaccgtgg caattatttc tttcctgata taagtggtgt aggattttca cataatacct
     7321 atgtatggaa aaaagagatg aaacctgaat cagggatgat ccgtcttgtt attggtcttg
     7381 gtacacgagc tgtcaaccgt gttgaaggtg attatcctcg catagttgct cttgacgatc
     7441 cacgctccaa acccctttat gattttaaag acgtacggcg attttcacaa cacaatgtag
     7501 atgttcttaa cataacaacc aatcaagtag aaaccataca gatggacgat ctattcaaca
     7561 ttcaagatct caatcttcca atgcaccttt taggaataag ggattatgaa gctgaacaaa
     7621 ctattaaaaa tatgaatggc gaacaaaaag aatattgggt cgttactttt gatgaactat
     7681 taatgcacac tgattttacc agtaacatta aaacaatctt acaaactatc cataatagat
     7741 atcaataccc tgtcgatatt gagtttaccg taaattttaa taaagaagag gggtattaca
     7801 tcaacatatt gcaatgcagg cccaatcaga taaaaggatt gggaaaaaaa gtgaaattac
     7861 ctgaagatat acccggcgat catatctttt ttacctcccg gggtaacttc ctggggggca
     7921 gtatagttca gcctatcaag cgaataatct atgtagatcc gtatctgtat aactcacttt
     7981 caatttccca aaaatatgat gtggcacgca ctgtaggcaa attaaacaag gcaatacaag
     8041 atcgtgaaga aatgccagca ttgctcattg ggccgggaag atggggcaca actacacctt
     8101 cattaggtgt tcctgttaga ttcgctgaga taaataacat taaagtactg gttgaacttg
     8161 cgttgatcaa cgaaaatata atgcctgaac tttcatttgg gactcatttt tttcaggatc
     8221 tggtagaaac cgatattttt tatgtagcat tattttatgg tataagcaac gttctttatc
     8281 atcaaaactt tcttgagcaa ttccccgata tttttactaa acttctacct gattctcaat
     8341 ataatcacgt tatccaagtt tttgatctaa aagaacacga tgtagaactt atggctgata
     8401 tcaccaccca gaaagtacta tgttttatga gttaaactaa taccattttc atactattgt
     8461 ttagctaaaa acgcttgatt atatttctat tagcctcatg tcaactgtac tgtcaccaac
     8521 catttttttt aataaacatc atgttataaa aaaatattat tgacaattat atcaattgtt
     8581 tagtgtgttc atttccatat atttggcatg ttttgatgat tcatcattat ctataagcaa
     8641 tggttatctt taatatcata caggaggcat tacaatgtca gtaatatcag aagtaataga
     8701 caaggttaaa aggcaggatt cagagcaaaa agaatttatt caagcagttc atgaggttct
     8761 ggaaacactt gaaccaactg ttcaaaaaca tcccgaattt gtgaaggcaa aaatctatga
     8821 acgaattgtt gaaccagata gagtaattat atttagagta ccatgggttg atgataatgg
     8881 taatgtccat gttaatagag gtttccgcgt tcagtttaac aacgccatag gaccatataa
     8941 aggtggtttg cgattccacc cttcagtcaa tttggggatt ataaaattcc ttggatttga
     9001 acaaatattt aaaaactccc ttacaacgct acctatgggt ggcgcaaaag ggggatctga
     9061 ttttgaccca aaaggaaaat cagatatgga tgtcatgcgt ttttgccagt ctttcatgcg
     9121 cgaactatac cgacatattg gtcctgacac tgatgtacca gcaggtgata ttggtgtagg
     9181 agcccgtgaa ataggattta tgtttggcta ttataaaaaa atacggaatg atcatactgg
     9241 agtgttaacc gggaaagggc ttgaatatgg cggaagcttg cttagaccag aagcaacagg
     9301 atatggtgca acctattttg cagcagagat gttaaaaact gttggaaaag aaattaaagg
     9361 aatgactgta gccgtcagtg gctccggcaa tgtagcacag tataccatag aaaaggttac
     9421 acaacttggc ggaaaggtta tttcatgcag cgattcaaat ggaacaatca ttgatgaaga
     9481 aggcataact ccagataaat tagcatttct catggaattg aagaatgttc gccgcggtcg
     9541 tgttaaagaa tatgccgaaa aatataaaaa tgctcattat tttgaaggta aatcagtgtg
     9601 ggatgttatt aaagagcagg gtatcaaaat tagtgtggca ttcccttcag caacagaaaa
     9661 tgaaatagat ggcaagcatg ccgaagcact tatcaaaaat ggatgtatct gcgtttctga
     9721 aggagcaaat atgccttcaa atctggacgc aatcaaggta tatcgtgaac acaatatcct
     9781 ctttgcacct ggcaaagcag caaatgctgg cggtgttgct acatctggat tggagatgtc
     9841 tcaaaatagc ttacggttat catggacacg agaagaggtt gataatcgac tacagattat
     9901 catgaaaagc attcacaaaa gttgtctgga tgcctcagaa gcttatggca aaaaagggga
     9961 ttatctcacc ggtgcaaaca ttgcaggctt cttaaaggtt gccaaagcaa tggtagccta
    10021 tggcatagta taaacactgt cctataaaaa caaatagagc aggatacaac cctgctctat
    10081 taaaataaaa ataataatat ttttatgcgt acattttatt aatttcatct ttaaacactt
    10141 caactatcac ccgcttttta gttttttgag ttggtgttag catcccatta gattcagtaa
    10201 accgctgatt caaaatggta taattcttaa tctgttcaaa cttttccaga tgtttatttg
    10261 cttcttttac atcgttatca atcaattctt ttaagcgata gttgttaatg aaatcatccc
    10321 caacctgtac gcaaagctgc atgccaccaa catcttcata taccagtttc gactcatcat
    10381 atgtaatacc ttctcgctta aacagcgcca tgaagttggc aaaatttggt acaattaagg
    10441 cagtaataaa tggcttttca tcacctacaa gaaatatttg ttctacccag ttacttgtag
    10501 aaaataaatt ctcaatcttg gcaggtgcaa catttttccc aatagaggta cagattatcc
    10561 cttttttccg ctcaatgaat ttaaaataac catcgcttga taattctaca atatcccctg
    10621 tcttaaacca accatctttg gtaaaagttt ctttcgtttc ttttggttta ttgagatagc
    10681 ctttgaaaat accagcacct gaaagttcca gttcaccgtc ttttgaaaca cggcatttgc
    10741 tcccatttgc ctcttttccc atataccctg gtttacatgc tgttattgga ttgagtatcg
    10801 cagcattgaa actttctgtt gaaccatacc cttctatgac tgccaatccc agcgcatagt
    10861 aaaatcgcaa aagatcaggc gaaattgcag cacttgccga aaatgaatga tggaatcttt
    10921 taccaaacaa accacgcacc tttgcaaaaa ttttatcagc aatggaatat tgaatcttaa
    10981 gccatagtgg cagcctggac tttagatcaa aattcggatg catattataa cacccatttt
    11041 catcctcacg atatttcaaa gcctgtaatc ctacattcaa tgcccaatta aataattttt
    11101 tttgtaaagg gttctcagcc atctgctgct gcatggttat atatattttt tcatacagcc
    11161 gtggaacaca attaatccat gttggattgt acttttgcat atcctcaata agggttgctg
    11221 gcttgtcggc ataacatatt aacgaccctt tactgatagc cagccattga catgaaccgc
    11281 ggtcaaaaac atgcgaaagt ggaagataac acagtgtaac atcattctca ttaacatcaa
    11341 aattatacct ttgaaaatat tccgttgttc cctccaaccg tgatgaacca caccaatggg
    11401 ttaaaataac acccttcccc tgtcctgttg tacccgatgt ataaagaata gtaaaccaat
    11461 catctaatgt tacactatct ttgcgtttat tatatttttt ataatttgcc tgattatttc
    11521 gccattcccg tcccatggca atacaatctt taataccgaa taccttcttt ttatcaccct
    11581 taaatgacat atccatcact attattgttt taaggctttt caaatctttt tctagttcct
    11641 taacctgttt aagaagtgtt ttatcgccaa caaaaacaat agtacatctg gaatcattca
    11701 taatatattt aacttcatta tttgaaagcg taggaaaaat tgtaatactt gcaccacctg
    11761 cacatgcaat tgcgatatca acctgtgtcc acatatagct tgttggagct aaaatagcag
    11821 ctcgcccttt ataaggaaaa cctaatttca ttaatgcaca tgcaatatcc tcaatccgtt
    11881 ctttaagttc agcataagta aatagcccgt tgttatcatc atacagctct ttattaaact
    11941 tttgtgctgg cctgtccggt aacctatcac atgtatcata aaaataccgg accattgttt
    12001 tcagttcgcc cataatgccc ccttgaatat attaattata atatacatat ttatatatca
    12061 cgaccagatg gtatttcaaa aattaatgac aaattaaaaa atatgttatt taaaactatt
    12121 caatagtatc tctttacaca tggataaagc aatcccaaca ttttcagggg atttaccacc
    12181 tgcctgtgcc agatctttcc tgccgccgcc gccaccacca atcttctttg caacagcttt
    12241 aatgatagca ttgcaatcaa tacctttatc tacagctatt ttgttagcag cagcaataat
    12301 aaaagccttc tcatcatgtg aagaagcaag gcacaccacc actgcaacgg atagctcccg
    12361 aacaaaatca gccactgagc gtaatgcttc agcatccatg ttttgtaata cagcgcttac
    12421 aaattttatg ccattaattg tttctgcttt gtcatataaa tcttttgcta tacttttaag
    12481 tcccttttct tttgtttttt ccagttcttt ttctaataac gataccttct ctaccatctc
    12541 agttattttt ttctctacat ttgattcatt tgattcatta acctcaagca aacgcatcac
    12601 cctgccaagc aggtgctgat catgtaacag acgctcatat gctcctttaa gcgtcacacc
    12661 ttcgatcctc cgtacccctg cacccggtga agcctcatgc attatcctga aaacaccaat
    12721 ttttccggta ttatctacat gcgtaccacc gcaaagctcc ttgctgaaat tattaaccga
    12781 caccatccgc acaatgtccg aatatttttc accaaacaca gccattgctc ccgcatgtat
    12841 tgcatcctgc aatggcataa cactggtttc aaccggcaag catgcccata tcttttcatt
    12901 caccatagtt tcaacctgca ataattcatc atgtgacatt gccttaaaat gggtaaaatc
    12961 aaatctaaaa cgctccggtt caaccaatga acctgcctgt ttaacatgct gccctaaaac
    13021 attgcgtaaa gctgcctgca gcagatgtgt tgcagtgtga tttgcccgta tacaatttct
    13081 acgtattcca tcaatggaca agcgtacatg ctccccttct tgtaaatatc ctttctccat
    13141 ctgtcccaca tgaataatga ggtcgccgct tttttttgta tcctctactc tgaaagttcc
    13201 attatcacca tatatataac cagtatcacc tacctgccca cccgactcac cataaaacgg
    13261 tgtattcttg acaactatta tacccctctc accatctgca agctgtacct tcaaatcttc
    13321 aaaatttgac atggcaacaa catcagattc cgaatcatat ttttcatatc cttcaaactc
    13381 tgttctctgg ataatctttg ctaactgtcc aacaatagta tcaatacttc cttctgcacc
    13441 tttccagctc tggcgccctc gtgtacgttg ctcttccatc tttttttcaa aaccaacttt
    13501 atcaatagtc atcccatgtt cctgtgctat ctcttctgtc atttcaaccg gaaagccaaa
    13561 ggtatcgtac aatacaaaga catcattccc ctgtattgtt ttttctccct tctttttcag
    13621 ggcattaact atttcatcca gcctgtccat accattttca attgtttcta aaaagcgttc
    13681 ctcctcacct ttaataacag ctttcacatt ttcctttgtg gacgatagct ccggatattg
    13741 ttttcccatt attgtaacaa taggctccac catggtataa acaaatggct catggatacc
    13801 aatctgacga ctatagcgca atgctctcct gagaatacgt cgcaaaacat acccgcgccc
    13861 ttcatttgag ggatatgccc catccgagat ggcaaaagtc agcgttctgg catgttcaac
    13921 aagaacattg acagcaacct gtgcgtctcc ctcatagcga atattgcgtt tgtttataac
    13981 atagtttacc aattcattga gttcatctgt ttgatagata ctatcgacat tttgcactaa
    14041 tgttgccagc cgttccagcc ccatacctgt atcgatacca gttttaggaa gtgggtgaag
    14101 cttcccttca gtatcctgaa aatactgatt aaacaccagg ttccagaatt ctaaaaatcg
    14161 ttcacaatca catcctggtt tacagtcagg cttaccgcac ccaaagcttg cccctctatc
    14221 cagatacagc tcagagcagg gaccacatgc ccctgaatca cctgcaggac cccagaaatt
    14281 atcctctttg ccaagacgaa caatcttttg ttcaggtatt ccaatgatgg tatgccatat
    14341 atcaaatgct tcatcatcgt cggtatatat agaaacccag atattctctt ctggaaattg
    14401 aataacctgt gtggaaaatt cccatgccca ctggatagcc tctttcttaa aataatcgcc
    14461 aaatgaaaaa ttacccagca tctcaaaaaa agtgcagtgc cgttttgttt tcccaacgtt
    14521 tgctaaatca ctggtacgta aacatttttg tatagaagca gcacgtgtgt attctgcatt
    14581 aattgtacca gcaaacatag gtttaaactg aaccatgcca gccgtggtaa agagcaatgt
    14641 aggatcatcc tgcggaatga gcgagcttga tggaactatc ctgtgatctc gctgtttaaa
    14701 gtattcaata aaagcaactc gcaaatcatg aactttcatc ataatgctga attcctctct
    14761 ctcaataata aaatagtgta gtgattatct tgttgaacct ttccctgtaa caatgcatgg
    14821 ttgtttttgt atctcattgt tgtatacttg taatcaattt cataaaatca atattattat
    14881 aaattgtgat taaccctctg catcggctac tcaatcaata acataacaac atatcaaatg
    14941 agaacaatat aaaaaattgg tgctacaatt tcaatattat ttatttccac aaaacgattg
    15001 tatcatggat tgcctaatca tcaataaaat tattattatc actattatta tcaccaagcc
    15061 tgttcaaaac ctcttttatt gtatcccagc taaaacctct ttgctgcaaa aacaatccaa
    15121 ccttttccct ggcatgctct tttcctttta cacgattata ttttttttgt gctatctgat
    15181 atatgcgctc aatatcaggc tcgttatcct gcattttttt tattgctttt tttattaacg
    15241 atttatggat gcctcgttca aacaattttt tttctatcac cctgttgcca acaactttgt
    15301 gttcatgtgt cgaacggaca tactgtaatg catagtgata gtcatctata aatccttttt
    15361 ctataaataa ttttataact ctatatattg tattcttatc aaatcctttt tttataagat
    15421 attttgttat ttcatgctcc gatctatctc tcagcccaat atagtaaaga gctttttcct
    15481 tacattcaaa cagctttgac tcttctttaa tatgttcata tgcatcaata tcaatttcag
    15541 taccctttcg cagcaatccc tgtggtactg aaacagaatc aatttttaaa tgctcaccac
    15601 tatctaacgt tatcatgata tagcgatcag aatattctat atctattatt accatgaaag
    15661 ctcaccatac tattaatatt taacaataaa aaaggatgct cagcggcatc ctttttgtaa
    15721 agaaaaattg tattctcatt attatcgttt tgaaaattgg aatcttttcc ttgctttctt
    15781 ctgtccgtat ttctttcgct ccacttcccg tgaatcgcgg gttaggaatc ctgcctgtct
    15841 cattgatttt ttatactttt catcaagcgc cgacaaacat cgtgctatgc ctaactttac
    15901 agctccagcc tgtcctgtcc atcctccccc atttactgtt gcatacacat caaatgaagc
    15961 aagtaccccg gcttcttcaa gcggctgtcg tacaaccaat tcaagagtct gtcgtctaaa
    16021 atattcatta ataggcttac tgttgataac aatagcccca ttccctggtt tcaaccaaac
    16081 tcgtgcaaca gctgatttcc ttctccccgt tgcataatta actgcgtttg ccatagtacg
    16141 tacctcgtat ttatttatac ctatagattt atcttattaa aattttaatt ccagattttc
    16201 cggcttttgt gcttgatgag gatgatcctt ccctgcataa atttttaaat ttcccatgat
    16261 attgcgtgac aatttatttt tgggcatcat cccttttaca gcatgatata aaacatattc
    16321 cggttttttc tgcaacatct ttttaatatt tacaaatttc ataccacctg gatatcctga
    16381 atggaagaaa tactctttat catctggttt tttacctgtt aataaaattt tttctgcatt
    16441 gattacgaca atattatcac ccatatccag atgtgcagaa taggttggtt tgtgctttcc
    16501 tcgtaaaaca tgtgcaatct ccgctgcaag ccgccccagt acttttccct cagcatcaat
    16561 aatataccat tttttctcaa tctgagattt ctttaatgaa aatgtccttt gccagatact
    16621 cattgtagcc tctcacactt cgatgttaaa tgttaataga aaatttctaa taaaaattat
    16681 atagattata agcgtagcac gctatatata ttaatcaaaa aactgtggaa aaaagaaatt
    16741 catctctttc agaatttggt gttcaaactt attttttgat ggtaaatttg tcaaggaatt
    16801 aaaataaaaa tttgatcttc atgataataa taattatcca tataatgggt tagcgtttga
    16861 taaaccttta atcctgatcg agaagatgga ttaactataa cagttgcttt cattatttta
    16921 tcctgtacct gtttgttgat agtcataaca actgccttaa aataataata tttataactt
    16981 taaaaataag ggggcgataa cgtgccccct tattataaac aaatttttaa aatttcttta
    17041 aatttttttg atatatggct tttgcctctt taactgtccg ctcgataaca tcttttactg
    17101 ctggaacatc atttatcaaa ccctgacact gtcctatgag ctgtacacca tattctaaat
    17161 taccatcttc agtagcaact ttaattttat caaatgctgt tgcaaaatgt gccagctgaa
    17221 tcatcatttt agggccctgc aacataactc ccaataccaa tttaaaatag ggcaggttca
    17281 tcatctttgc tattttatac gattgtatag cagcatgagg cagactcata ccttttttta
    17341 ttgctttttc tgctgatttt gtctttaaca cacgacaata gatgccatca aatctgttgg
    17401 aatatatggt atcttctatc cctgctttca actgggcttc aattgttttt ttatggagag
    17461 ggctttcttt tgtcattgac aatcgggtac ccattcctat accttcagct cctaatgcca
    17521 atgcagctgc cattcctctg ccatcagcaa ttccgcctac agcaacaaca ggaattttaa
    17581 ctttatctac aattgcaggt acaagaacaa aggtagtaac ctgtccacca tgtgctgccg
    17641 cctcatgccc cgtaacctgt aatgcatcag caccctgttt ctctgcagcc aatgcatgct
    17701 tctcagtaac aacagttgca ataaccttac cgccgtattt atgagccctt tcagcaatcc
    17761 aatcacactt acccaatgaa acattaataa tcgggacctt ttcttccagc ataacctctg
    17821 cattttctct tgctcctggc atgatcagtg tacatcctgc accaaaaggc ttattggtta
    17881 attcacgtat tctttgaatg gctgctcgtg tctgctcagc tgttaaaggt cctgttgcca
    17941 gataaccggt gccacctgca ttacaaaccg cagcaacaag ctcgggtgtg cttatccagc
    18001 tcatccctgg taaaataatt ggatacttga taccaaaaag ttgtgtaatt cgtgttttca
    18061 taataactcc ctttttgcta ataatcgtaa ttcaatacac taaccataac ataacaatgt
    18121 tatggttata ttatttcaat caatatttta tatacaatat tatttttttc cctattaacg
    18181 cttaatcttc atacatcttg ttaatgacat cactatatcg ctgttgaatt actttacgcc
    18241 gtatcttcat ggttggtgtt atctcaccat tatcctgagt aaactcagca ggcataaggg
    18301 taaagcgctt tactttctct acctgtccta aaccttcggt cagcttttca attttattgt
    18361 tataaaactc aagtattccc ggatgagata ttagttcctg gcgtgatgaa tactgaatcc
    18421 cttttgattt tgcatattca tgtaacatct caaaattagg aactatgagc gcacttatat
    18481 atttccgtcc gtcacccact acaaccacct gctcaatgta caaatcctct cctaacatgg
    18541 tttctataac ctgtggagcg atgttcttac caccagcagt aatgataagg tcctttatac
    18601 gatcggttat gcgtatatac aggttatcat tatgtttttc aacggtccca acatcacctg
    18661 ttttaaacca gccatcagat gtaaacatct cccttgttgc ttccggttta ttatagtagc
    18721 catccataca cattgggccc ttcaattgta tctcaccctc atctgaaact cgtacatcaa
    18781 caatatcaag aacaggaccg catattccgg gatatgctgt tttatcagta cccactgcaa
    18841 cagggaaaaa ttcggttaag ccataccctt gtgccannnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnncaat aatggttttc accaccttta aggagtaccc cataactcca
    19021 catacgttca aaaacatgac ttaacggcaa aaatgccagc gataccatac ccggacctgc
    19081 ttcaggaaat cgcactactg aagcataaat acctgctaaa aagcttgcat gggtatggac
    19141 agcccccttg ggattgccgg ttgtccctga ggtataaata atggtaagca tatcgttact
    19201 tgatagtccc cgcatgcggg atgtaacctg ttcaataacc gcactatcta ctgtagtatt
    19261 aataatatct tttacataaa aacccttttc actggttaca gtgacatcat cctgcagcga
    19321 tataacatga gtaacagatg acatggtggc aatttctaaa atcttatcaa attgttcctg
    19381 atcaccagta aataccacct tgattcctgc atcttcaata atatatcgag cttcatcctt
    19441 tgaattggtt gcatagattg gaacagggac acacccggaa gattgtattc ctaaatctac
    19501 tatggtccat tcaggacaat tttgcgcaaa aattgcaatc ttatccccag actgacatcc
    19561 atgggatatt aaaaaatatc ctattgcata ggcatggtgt ttaagctcag accatgtata
    19621 ggaatatttc tcatcaccct taaagccggt taatgcgata ctatcacctc gtgcatcagc
    19681 acgggatagt agcatagaaa caaccgtttt ttcttctgat aaaaaatcgt gtaatccttg
    19741 caatgttgct ttctgcataa caaacccttt tgcaataata ttctttgatg ataattctgt
    19801 cgtactcttc aatgactatc gtgctatttt actttttttg ataactgttt ctctatcgct
    19861 actgcttctt ttactattga atccactacc tgctttaccg ttggtgtatc ctttattatg
    19921 ccgcttacct gccccaatgg cagcacacct tttgcaaaat cgccatcaat ggtacccagt
    19981 ttaaatgcat caaatccaat tgccatctgt gccatttgca tcgatcgttt tggccccatg
    20041 agcataatgc caatcgctaa cttaagccat ggaaactcta acatatcggc aatctttttg
    20101 gatctgataa gtgcactgaa tggatttaat cgttttttca ataaatgctc aaccccttta
    20161 gacttcatga acctggcagg caagccatca acttttggtg tataaatagt atcatatacc
    20221 tcacttttta cacttgcctg ttttgcctgt tctgcagccg gactttcttt ggtattcata
    20281 aaacgagtac ccattgctat gccatctgcc cccagcgcca gcgcagctaa aagccctcta
    20341 ccatcggcaa aaccaccagc agcaataata ggtatatcta acgcatcagc aattgccgga
    20401 ataagtacaa gcgaagtaat tgctccgcca tgcccggctg cttcatatcc tgtaacaatt
    20461 aatgcatcag caccatcccg ttgtgcagca aatgcatgtt tatgtgtggt aactgttgcc
    20521 actaccttgc caccataggc atgagctgct tttgcaatct tatcaccttt gcctaatgag
    20581 taatttataa taggaacttt ttcttcaatt aaaacctgtg cattccgttc agcaccgggg
    20641 aaataaaggg taacatttgc accaaatggc tttttggtga gcgaccgtat tttctgcacc
    20701 gcttcacggg tctgatctgc tgttaatacc ccggtggcaa gaattcccaa acccccggca
    20761 tttgatactg cagcaacaag ctccggagtg cttatccagc tcatccctga aagcactatt
    20821 ggatattgaa tcttaaatag ttctgtaatt cttgttttca ttgaatactc ctttatatat
    20881 aaatttgaat aagtatgcct tttctcaatt ttaccgcaaa cataactaag ttatctaaaa
    20941 aagcaataaa attatagtat tttgtaaaac tttcatatta tcagtgaatt agatggtggt
    21001 gtaatcaatc ctgcacataa catatggcag ataactatcg ccaatccatt cgcgctcttt
    21061 tgatagtact attgtatcat caagcatcct tgttacatca tcagccacca tacaattttt
    21121 aagccttccc tgtacctctc cgttattgac aaaccatgcc atatctaaat tagcagaaaa
    21181 ttgtcccatc aatgtatttg attgaccaaa tcctatgaat tggtcaataa tgataccggt
    21241 gtccatctgt ctaatcatat ctttgaatga tacatcccca cccggcacca caatgttgct
    21301 aaagctttcc tgtggcaatg tagcatagct acgcgatgca ttaccggttg ggttgcactg
    21361 taatatatgg ctactctgca ggtgcgctat atgccgttgt acatatccac actcaatgat
    21421 cgttttatta aaagccatca ccccttcacc atcaaaaggg taactgccgg gtgcaaaatg
    21481 ttcaagcgga ttatcataca cactcaactg cttgccaaaa actttctgcc caaattttcc
    21541 tatatagcgc gatatacccc gttgaatcga cttcccggaa agcccctgta gtaccacccc
    21601 cataatctgg ccaaatgctt ttggcgttag tatagcataa tagttgccag aagggatagc
    21661 agcattcgtt tcgctgaatt gcaacttcat aataagacga ttaacaattt catggtaatc
    21721 aaatgtgctt ccccatgaag tactttcata tacctgcata cggctcccat ccgatactat
    21781 gcgcaatccc gaaacgctaa ccccatgttg caggtaacga tatcccgctt caaaaccact
    21841 ggagttggtc aaataaacat atccgtcaac acaccctgcc gaactatcaa ccatgcaatc
    21901 actatatgct ttacggattg tatctatgat agcttgcact tgctggacct gattttctac
    21961 agtccagctt tccaaccatt gttcatcata gagcgttact ttaggaatgg catttttacc
    22021 agggagtgca taatcctcgt tatccccata ctgtgcagta tcctttgcat gggcaatgac
    22081 agtttttata tcattgaaat cattacaata tgaaaaaccg gttttcccat gtatgttcag
    22141 gcggatacca tatccccgta ctgcagattc gcgcgctgaa tgaaatacat tatttttaaa
    22201 ggtaactgac ttactgatag tatcattgga atagatatcc caccatctgc attgaacgcc
    22261 ctggcattct ttgcgaatag catcatgatt tctttttgct gcctccagca ccgctgcctc
    22321 ctataagaac attttttatc aataggtgcg gcccacctga agaaaccgga agtggtgcct
    22381 gcccctgctt gccacagcct cccaatccac caaacatggt acaatcatct gcaattgaat
    22441 caatattcat aagcgtcatg aacacattcc cgctgagcat gcaatcgcgc aataatctcg
    22501 ttttctttcc ccgttttacc tcataggcat atgccggggt aaaggtaaac atctccagat
    22561 tggtctgccc acctaatgcg ccagctacat ataatccatt acctaaacga tcaaacagct
    22621 catcagcagt tgcggtgcca ttatcaatat aggtattggt catccgtaca atagggtgtg
    22681 caccacacga tattgcccgc gcattgccgg ttggctgctc acccattttt ttagctgttt
    22741 cccgagagtg caatctgcca gtcaaaatac catccttaat caaataggtt ttttgcggtt
    22801 gtatcccctc atcatctata gggatatacc ctgtataggg tagtgagcca tcatcaatca
    22861 cctgtaaaca ttccggacca aaacgcctgc caatcaccat gagctctttc attcggggat
    22921 tctcgtagat aaaatctgct tcagaaagat gcccaaatgc ctcgtgaata aacacccctg
    22981 ccagctttgg atccactatg acattgtata gtccactttg tacctgctct gcctttacca
    23041 gctctgcagc cgttttcacc acctcttctg ccacagcgtg attatcctca accagttcaa
    23101 atccaccgta ctgtgcaatc gattcaccgt acggttgcat aacagtacca tcctttgcta
    23161 tagccgaaaa agaaacgcca cagaacgagc tttcataatc aacacctgtt tcgtcagtat
    23221 tgtacatata atagtgagtc atggaatccc tgtatacaac attggtattc tcaaccaatg
    23281 ggctgctctt taaaattgca ttatagcggt gcataagctc catcttttct tcaaggctta
    23341 cgctgtgcaa aggcttcagt acttttgttt ttttgttcac tacattggat tgcatggcag
    23401 caatggaaac agcgtcatct tttttttgta tgtgcgatag ttcctgtgca cggattattg
    23461 cataatctat catatccagt gttgtaaatg aaacaaatcc ccagcatccc ctgttataga
    23521 cgcgcaccga tccctgcgta gttttaccag ctttacaggt ttccactcta ttctttgaaa
    23581 gatggatgct taccgtatca ctttcactca ttcgaatttc agcatactca gcctttatgt
    23641 tttttaattt cttctgcgcc atcgtcatat ctactaccat tatatccttc tcccaaaaac
    23701 agtattgata ctcagaccac tgtcaaacaa tagtcatcgc taccctatct cggcatggat
    23761 ggcagatttc ttattgtgta tggtatagca aaaatccttt acactgttgt cattgccctt
    23821 tttattgcct gcccaatatt gctggataac tgtcgccaca aatggtttcc ctgaaaatat
    23881 aaatcctggt taacctccac ttctatgccg cagtacctat ctccgtactg ctttctaagc
    23941 cataccgtta caccgtcacc acaccccctg aaagggtaat tgtatgctat gcgcaacgga
    24001 tgcagtgcaa acaaagtttc tttgatactt ttacttaatt gctgttctac cgggcagtat
    24061 tgatcatata aaagtcctat atcacaatac cgtttaaatc cattcaattg tggtgtaaac
    24121 gaatggacag caacatgaat gacatgcgta tgatgtcgca ttgccctgct aacagcctgt
    24181 tctattgtat tgcggtgttt atcgtaataa cgtcgtatca gctcattttt aagaacgcta
    24241 tcaaaatttt ttgtaaattt cgaaaataat tcatcacttc ctactgaccg gttacaatcg
    24301 acaataaggc gtgtaacctt tgtgtatata agtggcacat gcagtatgct ggcaataatc
    24361 atagccattg gtaatgcccc aatatcatac gcaaggtggg ttgctactat ttcatgctct
    24421 ccggaaaaaa gtgctgtaag ctcaggtggt atatagtttc cggcatgctc acaggtaaca
    24481 atacaggtat tttttgccat gtgctaaacc tttgcagtat atcgcttgac ataaagcaat
    24541 tgtattctac ttttctggtc aacatttatt ttaacaaaag aggtctgtat gcttattata
    24601 acaactatta ttgggctatc agccggcatg ttatcaggac ttatgggtat tggtggtggg
    24661 attatcatga taccagcatt gatatatgta atgcatttta atcagcattt agcacaggga
    24721 acaacacttg ctgcaatgat accacctatt ggtattcttg cagcatatgc atattataaa
    24781 tccggctatg ttaatatacc ggttgcgctt accctggctt taggttttat cattgggggc
    24841 tattttggtg gtaagattgc ggtgtcactt gatagcgaaa cactccgccg tatttttgga
    24901 ataatcctct ttgttatttc attgcatatc atattttcaa aatagccgcg ctttactgaa
    24961 ccggtaataa tgtcattttt tattaacacc catgaatctg aaatttgtaa attttaatat
    25021 gtaatcatgt gtaatacata aaatagttgc tttattacta tagacatgat actgtattta
    25081 cagtatacac tttacatttg ttttttatca ggtaactatc atgcagcaac agaacagtgg
    25141 tattcaaaaa ccacaacgta taaaaccatt ctactcacaa aaagtaccca ttccatctaa
    25201 ccttacatct cttttatggg atgaacagga tactgctcca cttgaaaaat taattctccg
    25261 tgttttgcag tatggcaatt atgaagaaat acatactatc tactctttat attcaacgga
    25321 aacaactgac atagtatctc gttaccctga cataaaacgg ggagtaaaat tctgggtgcg
    25381 gtactggaat gaacaccatt aacacaacaa taattaccct ggcacaacag atacaagagc
    25441 agtttccgca tttttattta gctggtggta ctgccataat gttaaaatat aaccaccgga
    25501 taagttatga tcttgatttc tttaatttta aagaattttc atataaccga ttggcaaaaa
    25561 aagtaatacc attatatcaa aaccagattg ataaatggga gcgtaaagag gataatatag
    25621 atttctttat caatggaata aaagtatcat ttgttttttt tccttttaaa aaccttgaaa
    25681 aaattgaaac tattcatcac attaaagtag caggtgattt tgatctcttt ttgaacaaac
    25741 tgtatgttgc aggaagaaga attgacccca aagatccttt tgatgctgct tatttgtata
    25801 accagcataa ttgggataac aactacatta tacaaggttt tgaaaagaag ttcaagggac
    25861 aaagttataa aatatattta ggggctttat tgagctttga ggattatggc gaattaccag
    25921 catggataaa agaaacactg atgaagctta tataccaggt ttaaacctct tgagcatcaa
    25981 cgataaactt accactgtta ccgaacttaa agccatagca gcccctgcca gttcgggttt
    26041 taccagccag cctgtgaaag gatacaacac gccagcagct acaggcacta aaaccgcatt
    26101 atatgcaaat gcccagaaga tattctgttt tatgcgtcgc attactttta ctgctaactc
    26161 aaaataggtc accacatcac gcaatctgtt cctcaagagt actacatcac cagcttctat
    26221 cgccacatca gttccactgc caagtgcaat cccaacatca gcctgtgcca gcaccggcgc
    26281 atcattgatg ccatcaccaa taaacgctac aatctctcca tccttttgca actgtttcac
    26341 aacattcatt ttatcttcag gtttaacatc agcatataca gcatccatgc caatttcatg
    26401 agctatcgcc agagcactca gcctgttatc acccgtaagc atggctgttt ttataccacg
    26461 ctgttgcaaa gagcgtataa ccgtgtttgc atctgtcctg ggcgtatccg atagtgcaat
    26521 aataccatac ggcttttcct gtgctacaac aacgaccgtt tttccttcat tttcaaattc
    26581 tttcatcttt tgtgcgatag ttacttcaca tggtatgtaa tcctgtataa aatctctgct
    26641 gccagcaata accttttttt gagctatcgt ccccatgaca cctttccctt ccactacgct
    26701 gacatcctca acctcatgga acactgcatg gtgattggta gcatattgta caattgcttc
    26761 accaacagga tgctcagcat agcgttccag ggaagcacaa accgatagca gttcatgttc
    26821 actgatgttg tcaaacgcta taatattggt cactgtcatg gtgccggttg ttaacgttcc
    26881 cgttttatca aatactgcat gcgtaacctt tgaagcattt tccagggctt cactattttt
    26941 aatcagtatt cccagttcag cgccttttcc aataccaacg gttatagcag ttggagtagc
    27001 aagccccaat gcacatgggc aggctataac aatgacagcc acaaacacct caaatgaaaa
    27061 aaatatatcc ccatttatta cataccaaac cagtgcggct attgttgcaa tcgtcagtat
    27121 aaccggtata aaaatgctta ccaccctgtc ggcaaaacgt tgcacacgtg gttttgacat
    27181 cactgcatgt tcaaccagat taataatccg tgatagtatt gaatcctgtc caatagcttt
    27241 tgcctttatc acaatcaggc tgtttttgtt aagtgtcccc ccaataaccg cattgccttc
    27301 gcttttgaat actggcacag attcaccggt taccatcgac tcgtccacat aactttgtcc
    27361 ctttacaatt tcgccatcta ccggaatgcg cgtttgcggt ttcacaacaa ccgtgtctcc
    27421 aatggtaaca tcactggtgt ttatttctat ttcattccca tctcgaatga ctatcgctgt
    27481 atccggacta agcgatagta gctttgcaat ggtatgggat gtccttgatt tagccctttc
    27541 ctcaagatat ttcccgatgt taagaaatga tgccagaaat attgaggttt catagatcat
    27601 aaattcatgc gacagcacgt gcagtgaagc aaggatactt gcaccaaatg ataccccgat
    27661 gcccatggca tacataacat ccattgataa aaccctattt ttcagtgcat ggtatgcacc
    27721 agtaaatatg tgatagctca caaaaagaaa tactggcgtt aatataatag cgatgccaat
    27781 agtatccata gcccacaatc catacatcat tgccataagc aaagcaccag ctaccagccc
    27841 agcaacaatc tgtatcaggc gcagtggtga ctgtttttgt gtttggacct cttctgtccc
    27901 taaaaacctg taaccagcat cttcaacctt tgtttttatg tcgctgtcat gaacagcttc
    27961 aggggtaaca acaagcattg ctttccctgt ggcatagttt acattaatat caagcacacc
    28021 ctctatatcc ataatcgcat tcgttaccgc tttagagcac atgacacagc tcatgtcacc
    28081 tattgttgcc tgtacaattt ctttttctat gtatactcca ttttcttcca gcagttcaac
    28141 aatgtgtatg ggacttataa cggagctatc gtactcaatt tcatagtgtt tattgttata
    28201 gtttacacca ctaatacctg gtgtacttgt tatcagtttt gtaatagctt cccgattatt
    28261 attttttatt gccagtaata cttttttatt catattaaac atcctctaaa atactgtatt
    28321 actattcatg gtcatataat atactataaa aacaaaaata aatcaatacc tgattattta
    28381 ttattatctg gaaaaaaaat ttatgcctat tcatgatgga ttattattat ttcaatcact
    28441 tcacccatat tctttgctta tcgggtcatt gccttttagt aacttttttt attttatata
    28501 gactcaatcc ctataaaaat cttatcatca cggtgttcaa tgtttgatgc attccttttt
    28561 atcgcttcat taataagcga tactgtttgt tcaacactta agtgcccatt ttcaagtata
    28621 aaatgcttta aatctttata ttggtttcca aaatagtctg tactaaattc ctcaattata
    28681 ccatcagtaa ataggtacaa tttatagttt tttttgatat caattatctt attttcaaaa
    28741 tttgaaaatt ttgaaattcc aattgcagga cccgtgcggg ataactcaaa aatagtgctg
    28801 ttcataacca aaaattgatc aggatgacct gccgaagcat aaagaagttt attattgttc
    28861 ccatcaatat caaccataat tgccgttaaa aacattttca ttgttttata ataatccgaa
    28921 aatactgtat tgagtttctt aaaaacttca gcaggggttt cacattcatc ctttaactta
    28981 tcatattcaa cttttatcaa tatggtaatc aatgatgcgc ttataccatg tccagttgca
    29041 tcggcaagaa acacgcgtgt ctttttattt ggtagagaag ctatatcata aacgtcacca
    29101 cctatttgca taaacggttc attgtacact gcaatcttta cgtccggtat gtgcattatt
    29161 tctttaggaa taattctatc atgaatagtt tttgcaagat gaacttcatt tgatatttga
    29221 tcatataatg atgccagctt tttatacgtt ttctctgtat cctgctgagc agatatggct
    29281 ttttcagtct gattcaaaag ctcttccgtc tttttcttta cttcagcatt taaattctct
    29341 gaaagatatt ctgttgtctt aaatgctctt gcaaatcgta atgacaaaac tgatgattgg
    29401 aacagaataa aaagcgcaag cccatattca gacataaaaa atgtacgtat gataaataga
    29461 ttatgtaaaa tatcattagc aacagttatc attaatacta tataccctat aaatattaac
    29521 aatgcatcat ccctgcgacg aatactggca gcaataagaa ttataaaggc tccaatcatt
    29581 gtagtgaaca gtattacatt ataagcacct atcaaatttg taaatatttt agggggtaaa
    29641 atcattggaa tgcttataaa aataatccct gtaataatct gaaaaattac aaacattctg
    29701 tttgattcac gagggaaaag ataacaaaca tagagcgtaa aacttaacca tcctaaacca
    29761 aggctaccat attcaattga aaatctgata aaaaacacat cggaattata aaaaattcgt
    29821 tcaagataat tacctataac aaatgaatgg ctgaaaatag caaaacaaaa tatggaaaac
    29881 caaaaagagg ttttatcttt tctacgcccc atccagagaa ttaaatgata gacagcaatg
    29941 atgaatataa tgccaagtat aaccgtttta ataaaatcat gttcccacag aatctgtgta
    30001 atactttcaa aaggaccaat aactatacac ttattaaggc cgcctccacg atgaaaaaag
    30061 tttgatactt ttactgctat gacaaattct tcagtttctt tttcacagtt taaaggtttt
    30121 aattgaggtt ttaaacaagg gcttgaggat tgctcatcaa tgctaacaat cccagaacac
    30181 ataaatttgt tgccattgaa atagagttca taggctgtgt ttgcacctgg aacatagatg
    30241 gctggtgaag tttcccaaaa acttttattt acttttacct
//
LOCUS       DAOSOX010000010        29601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1574_length_29601_cov_8.778346, whole genome shotgun sequence.
ACCESSION   DAOSOX010000010 DAOSOX010000000
VERSION     DAOSOX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29601
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_29601_cov_8.778346"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLX22_01500"
     CDS             complement(<1..256)
                     /locus_tag="PLX22_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18608.1"
                     /translation="MKRKILQIVPCILLIGVVFFVFNNFSCSNSEPSVFGNNSSSPLK
                     GHQETARAIEIQDAFRKIYELYKDRVVFITTEQVVELPPNP"
     gene            complement(409..1074)
                     /locus_tag="PLX22_01505"
     CDS             complement(409..1074)
                     /locus_tag="PLX22_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18609.1"
                     /translation="MNKLIIIPEDKLIAYCLNLSHPALESVVIRECVDPLCNGDNPSL
                     YRRHFSLFHALYTIKTCPQYFHLMVYIHPMDIVIRSYPTNNACIHYDPWCDAFCNRET
                     VDGIYCSFHYEFYEPYNHNCLYDPLMKFYCDSYNINYEFSVELNKVYHGFKKYIASLK
                     EVEKALFFFQFNAIDTCNRSLLQKRYRELVKKYHPDRYNGDMAMIKQINEHYSLLKEI
                     VVI"
     gene            complement(1067..1264)
                     /locus_tag="PLX22_01510"
     CDS             complement(1067..1264)
                     /locus_tag="PLX22_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18610.1"
                     /translation="MKKIVICLIISILFFGVSCKKNEDNKVPMEQIQIDGSHDFSIIF
                     KDIIEKEKKDASRYKKGPFDE"
     gene            1420..2010
                     /locus_tag="PLX22_01515"
     CDS             1420..2010
                     /locus_tag="PLX22_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18611.1"
                     /translation="MRISNDLPVPSMMVPSTGRKTGVPVAQVNNVQHHVSLQKTISAY
                     TVVQEAHKLVTMALNISFELIQKAYSKESSYTEIIAKLATIDSYFNRLSPGTANVPAI
                     PPADVVAGSSQEITQLQKAVANNDIKTMNAIHNSLINNSQILESYKQTLSRALGVADN
                     DSPDIKVIKKIIEDNASNAFMVHQPLHHDNVKKLLS"
     gene            2038..2442
                     /locus_tag="PLX22_01520"
     CDS             2038..2442
                     /locus_tag="PLX22_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18612.1"
                     /translation="MELFDGFIAAIASEFKMKFNKTSEGAYTTTIEFENNRSQEILIT
                     LNKDESGDRIINYYSVIGTIKNESFELYKFSLKLNSTFDYGALALLNDTLILRNSLLL
                     KDCDPQRFMKSLVYIAAKADELEEMLITRNIY"
     gene            complement(2439..2963)
                     /locus_tag="PLX22_01525"
     CDS             complement(2439..2963)
                     /locus_tag="PLX22_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18613.1"
                     /translation="MVINKIPAILSLILFMTIEIVLPGMVYSDDTTTFNTLRISSEDI
                     PAGFMFGEIPQFAKKVFPENPCIVSTEGIQFLAGKLYPEGNPYNIKNMYVAIMSESSR
                     PFGDDIVCYVLMFKDQTSASSEIKKIQEFYKYNKDRIVLIIKGQIAIMLFADDINNYK
                     YIAELGNKLSSKLD"
     gene            3242..5329
                     /locus_tag="PLX22_01530"
     CDS             3242..5329
                     /locus_tag="PLX22_01530"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOT18614.1"
                     /translation="MSDVITTTPQFIRKRDGRIVPFDIEKITEAIFKAAKAVGGTDRN
                     AAVHIAESVVDILSILYKDEHIPTVENVQDLVEKMLIEKGHAKVAKAYILYREQHRKI
                     RESKDLLNEGIQLIEDYLDRSDWRVNENSNMSYSLQGLNNHIASAITAKYWLERIYPP
                     EIRDAHTNCDLHLHDLGLLSAYCVGWDLRDLLLTGFGGVPGKVESRPAKHFRSALGQI
                     VNFFYTLQGESAGAQAFANFDTYLAPFIRYDNLDYAGVKQCLQEFIFNMNIPTRVGFQ
                     TPFTNVTMDLTVPRSIANEHVIIGGESMDAIYAEFQKEMDIFNTAFAECMFEGDARHR
                     IFTFPIPTYNITKDFDWDNPSIHLLWEITAKYGIPYFANFVNSDMDPDDARSMCCRLR
                     LDNRELRKRGGGLFGANPLTGSIGVVTINMPRLGYLSKSKDEFLASLSHLMDVASNSL
                     EIKRKVLENFTSNNLYPYTRFYLRDVYKRFKKYWINHFSTIGLVGMNEACLNLLGQDI
                     TTDEGKAFTGEVLDFMRSKIVMYQHATGNLYNLEATPAEGVSYRFARHDKNRFSDIIT
                     SGKNEPYYTNSIHPPVGYAHDIFDLLDHQDQLQAKFTGGTVVHCFIGEKIDDPEMVKI
                     LVKSIAYNYHIPYFTITPTFSICPVHGYIPGNHTYCPYPHTKEELEMFGIEVEIEDRE
                     LSKPIETTHRIVS"
     gene            5701..5892
                     /gene="nrdD"
                     /locus_tag="PLX22_01535"
     CDS             5701..5892
                     /gene="nrdD"
                     /locus_tag="PLX22_01535"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOT18615.1"
                     /translation="MQRNTEKKIPVEVYSRVVGYFRPVNQWNKGKQEEFHERKEFMAK
                     ELLKVVDHEYSGYTENITH"
     gene            5855..6550
                     /locus_tag="PLX22_01540"
     CDS             5855..6550
                     /locus_tag="PLX22_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOT18616.1"
                     /translation="MNIRGIQKTSLIDYPGAISTVLFTGGCNLRCSYCHNPSLAYNDC
                     NLHYYSNEEVIDLLRSRKHLIGAVVLTGGEPTIAKGILQFLENIKSLGMKIKLDTNGF
                     QPDILKKIIIKNLVDYVAIDIKTSPQKYSLLTKTKLSFNCIKESIRLLKDFDIQFEVR
                     TTCVPEFVTLEDLHSIKEQIGQVSHYYLQQFNNDHTMDEDLHRVKPYPEEILLLFRQF
                     VQTFATYCDIRGL"
     gene            complement(6953..9625)
                     /locus_tag="PLX22_01545"
     CDS             complement(6953..9625)
                     /locus_tag="PLX22_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18617.1"
                     /translation="MVKYCITIVLSLYIVAIVTVDASSDIIGHDTVTMYCSGDSQALA
                     EQYVNGAQNLTIPNLLILADSEHIQQIIGIEKTIELLELLSTNAQSPYEKMFINDIAD
                     ISKERFYKNDVKGNQRWYIYDNSKEPMPKSVISFQTQLDTLILPDKNKIAVMQSCAGY
                     IPVKQYTQILHATGLCSASFELHKPVSMYVFSNMEYAIFINGTKVCVNTTTTVKKDAR
                     IIHIEPQEGFSIAIYYKPDNDMYLKILFRDETNATMQLPATDTMYYNPVNCYEIFYEY
                     QNQLLSNYMSNHSPDNALQLALFSESIHSTEAFKYYHQALRSHNDFISYRLCSLLLRY
                     SRAFPDSILLLRTNVQKNIQPQTVLGGYFYDMLYSPESMNIAEIQWLPILIQTLYIQK
                     EELINKKADLDFLFKRFPQSHELAFTIASIYSSYDNHKATTILETYKNLNDKELTLLI
                     HCYTENKQFEKIVTILSGVREHTFFYDYINALIALGKYGEAKSALFKTIAQGFDTEAY
                     ALLATIAQLEGYDASMYSQKHHAIVNSLVVHPDYMKATFDDYVQNYFFKQLAYEIINH
                     TTNKLMYSCYAVRMVHDALYCTIYDAYHCTDISVFNTYQFSPDTENISYTLYAYNNEG
                     KMKSYTFYPGNYTIQQKIQEYLHDSHCEFFVIAISFRLRKNIDIMHIQQNYKQSPFTL
                     DIIVLSPESDVTISGTMRLNEITTNVRYIHHYTCNSNELLSPNNREFVIMAIQPQALI
                     TWIEEQNALFKRGIELLDIKEIQGNSIDEAIHEVLQELQTYTIADNVYGITSIVQWTL
                     LHKGTQLDAMLYARYLLAKKGIISCIALGDSHKKIVAKKNNLYFDTVFLYIPLTPDKG
                     IWLTYKNSYPSSEVLNISNILLIVGGEIISKQSN"
     gene            complement(9636..10997)
                     /gene="mnmE"
                     /locus_tag="PLX22_01550"
     CDS             complement(9636..10997)
                     /gene="mnmE"
                     /locus_tag="PLX22_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOT18618.1"
                     /translation="MQETICAPATPPVHAPIAIIRISGPESLRVASSIFKQHNKQTIS
                     TPRYAYYGTIFDGEYEIDDAIVIYYAQPHSYTGEDMVEIFCHGNPIIVNKIMNRLLAL
                     HVRIAQPGEFTKRAFFNGKMDLTEAEAINHIITARSEWEIATAIQQKHGSLRTTINKL
                     KQTIVELKADIECAIDFIDQDIEIISYQDAQQRIEDIIHTIQEIINRCKVGNNLSHGI
                     DIAIVGKPNAGKSSFLNCVVNHERAIVSDEPGTTRDVIKESVTIHGIQCNLYDTAGIH
                     DVASGIELLGVQKSYRIIDEVSLLFFILDATSGFTVEDSHILERISSKPYIPVINKCD
                     IASPEQINAINTQLPKKGIVFAATTGYGMDVLEHTVSDFIQSSYVNYTNSFIADIRII
                     QLLEQSSAGAHEVREQLLKQSPHEIIASALQDVIDTIGAITGEITVDDVLNSIFSRFC
                     IGK"
     gene            complement(10999..11640)
                     /gene="jag"
                     /locus_tag="PLX22_01555"
     CDS             complement(10999..11640)
                     /gene="jag"
                     /locus_tag="PLX22_01555"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOT18619.1"
                     /translation="MKVLDIEGKTVEEATKKALLQLKIDDMNKINIEVLDEGKSGIFG
                     FGISRPAKIRVYYQQQNDVGEKAKEIIEKIMSLMDVNATVKDFKESENKVYVELESLN
                     SGLIIGKKGKTLEALQFMINLLVNKVTSSEKKIILDIESYRAKRERALRKLSKEIALK
                     VARTGKPWTLEPMNPFERRLIHLTLQNDSKVTTKSEGQGIYRKVKIMPIEKGR"
     gene            complement(11797..13416)
                     /gene="yidC"
                     /locus_tag="PLX22_01560"
     CDS             complement(11797..13416)
                     /gene="yidC"
                     /locus_tag="PLX22_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOT18620.1"
                     /translation="MEKRALIAVLLSLLIWILWFYLFPPQEKKPAVQDKKVEVEQTVE
                     IKKPELKQLSKFLELSRLKEEVTVDSHYYKAVLSNHGAACTSFIDKTRNIELVVNKSK
                     YIARGIFDFPVHFTTGEFLLSTESDYTNWKLQKETDSIRFFTGAIVQNNPLHIVKTFT
                     FDEKGQFFTVSFSITNRAINEFSFPEGRLIYSPSDFLGPSMDFNNSYNELNSIYYLDD
                     DFEKARKGSGFFSNHEDIKEINGVVKWAGIMSRYYLLIMIPQGFQGSGVVYDNREHTG
                     FRTGIIVPAKPLKPGEKIEHVFKIYVGPKNKDKLLAVDKQIGDAADVSKWIEPIRDFM
                     MWCLLGINKLLGNLGWSLVIFSVITKIMFLPLTKRSTESMKKMQELNPVVQELREKYK
                     DKPDILNKKIMEVYKKNKVNPMGGCLPLLLQMPFFFALYSALINSVDLWQAPFILWIK
                     DLSLPDTVFTISGFNINILPIVMTATTYLQQKMSTGEASQQQKMLLFMPLIFIVIFWN
                     MPSGLVLYWIMQNVLQIAHQMVVNRKPKTEK"
     gene            complement(13527..13748)
                     /gene="yidD"
                     /locus_tag="PLX22_01565"
     CDS             complement(13527..13748)
                     /gene="yidD"
                     /locus_tag="PLX22_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014951464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT18621.1"
                     /translation="MKINIISLPILLIKMYKLIISPLLPNVCRFYPTCSSYAIEALSK
                     HGLCKGGFLSIKRILRCHPYCDGGYDPVP"
     gene            complement(13745..14110)
                     /gene="rnpA"
                     /locus_tag="PLX22_01570"
     CDS             complement(13745..14110)
                     /gene="rnpA"
                     /locus_tag="PLX22_01570"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT18622.1"
                     /translation="MKRRYSIKGRRSFKDVIVNGRRFYSKGIRMIVHIQPAHETLFIG
                     NESVSHFSIAVNKNFGDAVERNKIRRRIKSILNTLLPQIQQGFAVIIFPDKTCKAMPY
                     THLSGLVKQLLLRSGVLKQ"
     gene            complement(14136..14294)
                     /gene="rpmH"
                     /locus_tag="PLX22_01575"
     CDS             complement(14136..14294)
                     /gene="rpmH"
                     /locus_tag="PLX22_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014934381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT18623.1"
                     /translation="MKRTFQPSVIKRLRTHGFRERMSTKAGREILKRRRQKGRCKLTV
                     SDEKRNVK"
     gene            complement(14493..15296)
                     /locus_tag="PLX22_01580"
     CDS             complement(14493..15296)
                     /locus_tag="PLX22_01580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT18624.1"
                     /translation="MIPLLCPICKDSRSKQIANDIFLCKPCKILFNNAYQPSPYTADY
                     FGEDYKNQYGKTYEDDHDAIYTASLLRIEKIKKLLNNNLSEKSLLDIGCALGFFCKAA
                     KDSGFQFVEGIEISEYAAQYCKEKFSISVTTVDFEKFLTAKKYDVITAWYVMEHFANP
                     FAMFQKVYSMLHNDGIFACTLPSYFGPLFYCHRDIWIKNHPPDHRIDVSPRGITRLLK
                     NMGFVKVKCYPSGYHPERFCSLASKHKKIYIKLSDSICFSDTIMVIAKK"
     gene            complement(15302..15790)
                     /locus_tag="PLX22_01585"
     CDS             complement(15302..15790)
                     /locus_tag="PLX22_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOT18625.1"
                     /translation="MNQVKKIEKILESALNEDLTSYFLLIKAMRKHLPLFGSTKRITA
                     ALLKECIGDISKLDLSEITSLNMAKSDGTIVFEDIKNGKARIFINIGKNHKITPGDLI
                     REISKRSGIDGKLVGKIDIHSTYSFIEIPEQYAELVLVSLDKARIKGIPIVVEPAKKK
                     KQ"
     gene            complement(15883..16530)
                     /locus_tag="PLX22_01590"
     CDS             complement(15883..16530)
                     /locus_tag="PLX22_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT18626.1"
                     /translation="MKIKTALITRSKELVSEVRLVQPLDASIHGSINTLKDEGFDIIL
                     FDSEQIDISDAQFYLSKIRKRFSHVPVILIVRTGYLESINRDWFFDDFIIFPFRKGEL
                     KVRIDCLIGSEIEIKDAVIRVGNIAIEPDKYIVSVNNEKVSLTFKEFELLKLFIENKG
                     VVFTRQELLSRIWGIEYIGGTRTVDVHIRRLRSKIGDEFNNIIETVRNVGYKCKD"
     gene            complement(16520..17695)
                     /locus_tag="PLX22_01595"
     CDS             complement(16520..17695)
                     /locus_tag="PLX22_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT18627.1"
                     /translation="MELHKKINILIINYEFPPLGGGGGVATYDLALEWVKVANVHIIT
                     SSFKGLPPYEYINGIHVHRVKILFRKSRDAATFISMFSYLPAGFIRALSLFKKYKFDI
                     INTHFVVPSGPLGYVLSKLFKIPNVLSLHGGDIYDPSKKFSPHRNRFFKLIVQYLLNK
                     ADAIIAQSSNTRNNAFHYYKIKKDIGIIPLAFHPPVIPKSSRKLLGLHEKDFICITIG
                     RLIKRKAIDVLIKAIARINNQHVKLIIMGDGPEREYLEQLVQELALENIVTFLGFVSD
                     DKKYAYLSVSDLFVLTSLHEGFGIVFMEAMYCGLPIVTTNSGGQVDFLENEHNALLID
                     VGDIQKCAECIMRFKNDKKLYAQCSKNNKARITAYYAENVAKQYMDIFYQLVDMNEN"
     gene            18588..19607
                     /locus_tag="PLX22_01600"
     CDS             18588..19607
                     /locus_tag="PLX22_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT18628.1"
                     /translation="MSIKDILIYFYLPVIVFTVLCFCAIVVGIIYWIKKYKELLIILF
                     YFLIHAGMTAMYIYPFNVFAFSLQYAFFLFCLLMFCFSAVFMVLPDASNNSDSSYRAV
                     QCIVGKSILVYVSIVFLTPKEQLIISTVDCMVSLVFISVKPKPATSCVQPFAEKEKFI
                     FIIVITIVFLWISIAKIFLFHVLILGMTILVIVNVVLVCVIALKVYVYKDMDFIAIQQ
                     ENYCGMYEKHFKIVQIIKEHINEYYTHPIDKYTIALQCEMNPDYLCKIFKSNEGITIN
                     EYIKNIRLKKALETLSNTDRKIIEIALDVGYANLATFYRHFTQFYKISPNQIRIKKSN
                     NITTV"
     gene            19810..20139
                     /locus_tag="PLX22_01605"
     CDS             19810..20139
                     /locus_tag="PLX22_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT18629.1"
                     /translation="MIELTDKGNGTFVVKINMQEVHTLDVPDLKEKLQQAIVNKGIKR
                     LVVDLADVKMITSSGIGIFLNINQNLKSQLRLAAPTPEVQKVLELTKVTSMIKVFNSV
                     DAALQSF"
     regulatory      20282..20382
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20458..21618
                     /locus_tag="PLX22_01610"
     CDS             20458..21618
                     /locus_tag="PLX22_01610"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOT18630.1"
                     /translation="MKAEIIPGNSAGIVTTNYFTFAEPPDELLLVSGKKLGPITLAYE
                     TYGTLNSDKSNAILILHALSGSAHAAGYHSKKDIYPGWWDIYIGPGKTFDTSHYFVIC
                     SNVIGGCSGSTGPSSINPETGKEYGLDFPIVTIQDMVNAQYHLIKFLGIQKLLSVVGG
                     SMGGMQALQWSISYPEMVQSVVAIATAASLSAQGIAFNEVGRQAIMLDPNWNYGKYYN
                     NENPKNGLSLARMIGHITYLSEKLMHEKFGRRLQHSDIFKFHLDLEFEVESYLHHKGN
                     DFVQRFDANSYLYITKAIDYFDLKNDFNGDLANAFVNVNSNYLVISFSSDWLYPTSQS
                     REIVRALRMNSKNVIFTEIQSDKGHDTFLLPHEQLEHNIANFLKREFEKVHR"
     gene            21618..22241
                     /gene="metW"
                     /locus_tag="PLX22_01615"
     CDS             21618..22241
                     /gene="metW"
                     /locus_tag="PLX22_01615"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOT18631.1"
                     /translation="MKEYRVAYDLILKEIPYGARVLDLGCGDGQLLKLLQTTRKVKGF
                     GVEISPEGVSQCVEKGLYCYQGDIDEGLNDYKDNSFDYVIVNQTLQSTKKPEIVIRES
                     LRISLNAIFSFPNFAHYSLRLYISIYGRMPKNKLLPYEWYETPNIHLLTIKDFRDFCN
                     AFGYPIKKEMHFSTIRSHSVTIPIMPNLFAEYGFFVLDGGPFTSDTI"
     gene            22869..24758
                     /locus_tag="PLX22_01620"
     CDS             22869..24758
                     /locus_tag="PLX22_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT18632.1"
                     /translation="MKIQFVQLPLIDHGLQYITGNIPNAAATLHAFCELTSGGTMKGL
                     QLPYSIQNYGSDSMIVNHILHNEPTHVAFTCYLWNVERSLAIAKTIKAMASHIIILFG
                     GPEIQVNSYILKQHQPCIDIFVIGEGEYFFNHYYNNTCKQFIHMVNGNAVFIQGSDTF
                     IPLSQIVEPYTSGYLTPMHDKSLSVEIIRGCPFKCTYCLYSKNTSRVRALPPHKLADI
                     IKKSMSDEIQELYLLAPTFNQSSHFNDYLQTLISSRCSVSLHTELRADTITSNIAMTL
                     KKANFRSAEVGIQTLNKKCLQKIGRKTNPEKELQGIFYLKDAGINLQVGIIPGLPYDT
                     PESFYTTMDTLLDAGLGNELELYPLMVLPGTALYDEAIAAGATFLQKPPYYLIEGFNF
                     TQHDITAITCYFEDTTGFTFDTPYLPSFIFQQGELVSGACIDLTKRRSIPMEVFDYMQ
                     TSHITFYLLCDSYPQIEHAVELIIQQYPWDTMLCSIVLMYDAVLNDEDIASKVFTATH
                     NTFYCRMMYFQDTYDKLPVRIFQVFESLKTYLRAKDHYRCINPIIKLLRSNYDEITQY
                     HFAYPVPAVVAKGCAGIYGQWIKEQYAECIDIVSFEDDDDQNMIYKTLGIDGKPTIPF
                     TTLSI"
     gene            complement(24755..25042)
                     /locus_tag="PLX22_01625"
     CDS             complement(24755..25042)
                     /locus_tag="PLX22_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18633.1"
                     /translation="MVMLFIVYHNAVDEEVVEITKKYAKGYTKFVGVLGEGNKEPQLG
                     THIWGGLNNCLMVAIEDNKMKDAIASDIASLKVKFKDAGVSIFVLPLKEML"
     gene            complement(25055..28180)
                     /locus_tag="PLX22_01630"
     CDS             complement(25055..28180)
                     /locus_tag="PLX22_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT18634.1"
                     /translation="MKITDISVSRPVTVSILYIGIIILSVVSYYFIELDFFPDVEFPI
                     AVVYVEYENVGPKEIENSVTKPLEEAVGSVNNVDYVTSVSKEGMSSITIKFQWGTDMV
                     EAVADIRERIDMVRSRLPEGIENPVTVKFDISMLPIMGLAITGERERSYIKKYAEDNI
                     KNLIEQTDGVASAQVVGGEDLEAKVELIKSRMDAYRLSIDQIIQTLALENQNIAGGDI
                     KTAYTKYTFRTEGEFKSLDDMRNIVVAVRNNTPIYLRDIARVSFEPGEVKDIVHINGE
                     NGTVMLIQKQSGKNTVLTVRNVYKKLESIKPTLPAGFDIRPIFDQGEMIQFSIQNLLN
                     NLIQGSIISIAVVLLILRNIRASLLVGLSIPISVITSFVLMYLFDLSLNMMSLGGLAI
                     GVGMVIDNAIVVTDNIFRYREKGARLNEASKLGTDEMGGAITASTLTNLVVFVPFMFS
                     AGLAAQLFTDMALTITFSMMSSLFVALTLIPMLIAKFVPTIEHHYTGRSNVLQPLFDK
                     SEKFFIDLENFYGKAINWALDNKKKVIGFVGITFLIGLILIPLSGMEFMPERDDSRIT
                     IMATLPIGTNLDETTRIMKLIEERTLPIFKEEELKVVMVRAGYGKGTNAAFNDLTDYK
                     ARIQIELKMRKYRERSKAEIRDILRKALQGIPGVTYNFTYQSRGSSSIGMGSEGATIS
                     IEVYGYDFEQSNQYVQSITNSIKNIPGLKDIDISREEGLPEKVVVVNREKASKMGINA
                     YQVANLIKDNVSGFVASRYRFEGDEYDINVRLREADRQTVEQIKSLEIDTPMGKRIKI
                     GSIIDVIDKTGPTAIDRKNHERVVYINCKAEGRALSTVAADINEALTKIPKPQNFYVS
                     IGGSYEDMQDTFNDLLLVLILGGILTYVIMAAQFESLIRPFIVMFSVPTCMFGVSLFM
                     FLTGSQFSVATLLGIIMLVGIVVNNAIVLVDYTDILIQRGWGLREALVEAGTKRLRPI
                     MMTTLTTVLSLIPMAFSGGDGSEMSQPIGRAVMGGMSTGFIFTLIFIPVIYMIVETRR
                     EKYSAKHKAKF"
     gene            complement(28212..29138)
                     /locus_tag="PLX22_01635"
     CDS             complement(28212..29138)
                     /locus_tag="PLX22_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT18635.1"
                     /translation="MKKGRAKTILTIVAIIIALIASYRLVMFVVELINPQKQVEEEHV
                     VAVKAAKAVRMDITKTLDLSGDIVGKEVVHVFPQVPGKVMEIQVKEGQYVKKGQVVFK
                     IDRDIIGMEYMPAVVESPLTGYVGTIMVDRGMSVTTQTPLAQVVTMNQVEAVAQLIEE
                     QINMVQIGMKAKVRVVSFPNTSFTGTVYRKTALLDAASRTQEVRILIDNPQLKLRHGM
                     FADIQIILGFVKKAVIIPSDCIMSDASGNYYVFKVIDNKAFKQSITPGYVHKNITEVV
                     SGINDGDVVVTLGKENVKHGVKLLVYMEEINY"
     gene            complement(29167..>29601)
                     /locus_tag="PLX22_01640"
     CDS             complement(29167..>29601)
                     /locus_tag="PLX22_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT18636.1"
                     /translation="SFAPSGWNDNWMITFGATYKWGGWSPLDSTHAKAKQLESQTNQI
                     DLQLQDFIKSIRLDIQSGFLKLESASNSIYSQQGNVETAEESLKTATIQFRNGIIDNS
                     KFLEANVALIQAKTLYIQALYDYKVSQAELNKATGIDYFTIQ"
BASE COUNT         9368 a         5235 c         5109 g         9889 t
ORIGIN      
        1 aagggtttgg aggcaactca accacctgtt cggttgtaat aaaaactacc ctgtccttat
       61 atagttcata gattttcctg aaagcatctt gaatctcaat agcacgagct gtttcctgat
      121 gccccttcaa tggtgaactg ctattattgc caaatactga aggttcgcta tttgaacagg
      181 aaaaattatt aaaaacaaag aaaactacac caatgagaag tatacatgga actatctgta
      241 atatttttct tttcattagt tatctcctaa ttactataaa ttattaaatc aaaagttatt
      301 gtataatagg gacttaattt attcaataat atatggtaaa ataaaaatta ataaaaaata
      361 tacaccataa taatatatat tatcattgtc aatatataat agaaatattt atattacaac
      421 gatttctttc aacaagctgt agtgttcatt aatctgttta atcatcgcca tatcaccgtt
      481 atacctatcg ggatggtatt tttttaccag ttccctgtaa cgcttctgta acaatgatct
      541 gttacaggta tctattgcat taaattgaaa gaaaaataaa gccttctcca cctccttgag
      601 actggcaata tatttcttaa aaccatggta aaccttgttg agctcaactg aaaattcata
      661 attaatatta tatgaatcac aataaaattt cattaatgga tcatagaggc aattatgatt
      721 gtatggttca taaaattcat agtgaaacga gcaatatatt ccatcaactg tttctctatt
      781 acaaaaggca tcgcaccagg gatcataatg aatacacgca ttgtttgtgg gatagcttcg
      841 tataacaata tccataggat ggatatatac cattaagtga aaatattgtg ggcaggtctt
      901 aatggtatat aaagcatgaa acagtgaaaa atgcctgcgg tatagtgagg gattatctcc
      961 attacataga ggatcaacac attcgcgtat aaccactgat tccagtgcag ggtgtgacaa
     1021 attgaggcaa tatgcaatca atttgtcttc aggaataatt atgagcttat tcatcaaagg
     1081 gtcccttttt atatcggctg gcatcttttt tttctttctc gataatatct ttaaaaatga
     1141 tagaaaaatc atgtgatcca tcgatctgta tctgttccat gggtactttg ttatcttcat
     1201 tttttttgca cgatacccca aaaaacagaa tagatattat taaacaaatt actatcttct
     1261 tcatagagta taatccatta tataaatata ttcttatatt tacaaacata caacacatgt
     1321 tgtaattttt caatattgat ttttttgatc ttcaaaattt tactgttgac attattaatt
     1381 ccatatacta cacttactgg tgatcaggga ggggattaca tgaggatatc aaatgatttg
     1441 cctgtaccgt caatgatggt cccatcaaca ggcagaaaaa caggtgtgcc tgttgcacaa
     1501 gtaaataatg tacagcatca tgtatcgttg caaaaaacga tttctgcata tacggttgtc
     1561 caggaagctc ataagcttgt tactatggct cttaacattt cgtttgaact tatacaaaaa
     1621 gcttattcta aagaatccag ttatacagaa ataattgcta aacttgctac catagacagc
     1681 tattttaaca ggttatcacc tggtacagca aatgttcctg ctatcccacc tgctgatgtt
     1741 gttgcaggat catctcaaga aattactcaa ctacagaaag cggttgctaa caatgatata
     1801 aaaacaatga atgctataca taatagttta attaataact cacaaatact tgaaagctat
     1861 aagcaaacac tatcacgtgc tttaggagtt gccgataacg atagtcctga tataaaagtt
     1921 attaagaaaa ttatagaaga caatgcatca aacgctttta tggttcatca accgttacat
     1981 catgacaatg ttaaaaagtt gttatcataa ttattgatta ttgtgaaagg gtggaatatg
     2041 gagctttttg acggttttat agcggcaata gcatcagaat ttaaaatgaa gttcaataaa
     2101 acttcagagg gtgcatatac aacgacgata gaatttgaaa ataaccgatc tcaggagata
     2161 ttaattactc ttaataaaga tgaatctgga gatagaataa ttaattatta ttctgtaatt
     2221 gggacaatca agaatgaatc ttttgaatta tataaatttt cattaaaact caatagtact
     2281 tttgactatg gcgctttggc attattaaat gatacactta ttttacgtaa ttctttattg
     2341 cttaaagatt gcgaccctca gcggtttatg aaatcgcttg tttatattgc tgcaaaagct
     2401 gatgaattag aggagatgtt aattaccaga aatatttatt aatcaagctt gcttgatagt
     2461 ttgttaccta attctgcaat atatttgtaa ttatttatat catcagcaaa taacataatt
     2521 gctatctgcc cttttattat caaaactatc ctatccttat tatatttata gaattcctga
     2581 atttttttta tctcgcttga agcagatgtt tgatccttaa acatgagcac ataacatact
     2641 atatcatcac caaatggccg tgatgattct gacattattg caacatacat gtttttgata
     2701 ttatatggat tgccctcagg ataaagcttg ccagctaaaa actggatacc ttcagtgcta
     2761 acaatacagg gattttccgg gaataccttt tttgcaaact gtggtatttc cccaaacatg
     2821 aacccagcag gaatatcttc agaagatatt cgtaatgtat taaaggtagt ggtgtcatct
     2881 gaataaacca tgccaggtaa tactatttct atagtcataa agagtattaa gctcagtatt
     2941 gctggaattt tatttataac catacttcat acctctttgt agttaatata gtgtaactat
     3001 cggtaatacc atctcaaagt acaatttaaa tttttgcgta atatttatac tatagtgatt
     3061 tatataaata tatgtttagt gtttcatttt tttttgttac gtgacataat tatgattgac
     3121 ttttttattt tattattcta acgtaatata caaaaacata tcttatcaag ctaataataa
     3181 ccatcgcatt gtgtgctata cagtgatcgt gttggtttat ttcttttttg ggagtcttta
     3241 tatgtcagat gtaataacaa caacccctca gtttataagg aaacgtgatg gcagaatagt
     3301 gccatttgat attgaaaaga tcacagaagc aatctttaaa gcagcaaaag cagttggtgg
     3361 taccgatagg aatgcagcag tacatattgc cgaatcagtt gttgatatat tatccatatt
     3421 gtataaggat gaacatatcc cgacggtaga aaatgttcag gatttagttg aaaagatgct
     3481 cattgagaaa gggcatgcta aggtagctaa agcatatatc ctctaccgtg aacagcacag
     3541 aaagatacga gaaagtaaag accttcttaa tgaaggaatc cagctcattg aagattatct
     3601 tgataggtca gattggcggg taaatgagaa tagtaatatg agttattctc ttcagggatt
     3661 aaataaccat attgcttctg caattactgc aaaatactgg cttgaacgta tttatccacc
     3721 tgagatacgc gatgctcata ccaattgtga tctgcatctt catgatttag gtttgctgtc
     3781 ggcttactgt gtaggctggg acttgcgaga tttattatta actggttttg gaggagttcc
     3841 cggaaaggtt gaaagcaggc cggcaaaaca tttccgaagc gcattggggc agatagtcaa
     3901 ttttttttat acattacagg gggaatcagc cggtgcacag gcatttgcaa actttgatac
     3961 gtaccttgcg ccatttatac ggtatgataa tctggattat gcaggtgtta agcagtgtct
     4021 ccaggaattc atctttaata tgaacatacc aactcgtgta ggattccaga cgccatttac
     4081 caatgttacc atggatctga ccgtacctcg ctcaattgcc aatgaacatg tcattattgg
     4141 tggagaaagt atggatgcaa tctatgctga atttcagaaa gagatggata tatttaatac
     4201 tgcttttgca gaatgtatgt ttgagggtga tgcgcgacac cgaattttta catttcctat
     4261 accaacgtat aacatcacaa aagattttga ttgggataat ccttccatac atcttttatg
     4321 ggagataacc gctaaatatg gtatcccata ctttgcaaac tttgtaaatt ctgatatgga
     4381 tcctgatgat gcccgctcga tgtgttgccg attaaggctt gataatcgtg aactgcgaaa
     4441 acgaggtggt gggctttttg gagctaatcc tttaacggga agtataggtg tggtaaccat
     4501 taatatgcca aggttaggat acctttctaa atcaaaggat gaattccttg caagtttatc
     4561 acatttgatg gatgtagcaa gcaattcact tgagatcaaa agaaaagtct tagaaaactt
     4621 tacttctaac aatctttatc cctataccag attctatctc cgtgatgtat ataaacgatt
     4681 taagaagtac tggataaatc atttttcaac aattggactt gttggtatga atgaagcctg
     4741 tttaaacctt ttagggcagg acattacaac tgatgaagga aaagctttca ccggggaagt
     4801 gctggatttt atgcgcagta aaatagtcat gtatcagcat gcgacaggca atttatataa
     4861 tcttgaagct acccctgctg aaggcgttag ctataggttt gcacgacatg ataaaaatag
     4921 attttcggac attattacct ctggtaagaa tgaaccatat tatacaaatt cgatccatcc
     4981 accggtggga tatgctcacg atatatttga tctccttgac catcaggatc agttacaagc
     5041 aaagtttacc ggcggtaccg ttgttcactg ttttattgga gagaagattg atgatccgga
     5101 aatggtaaaa atactggtaa aatcaatagc ttataactat cacataccat attttaccat
     5161 tacgccaaca tttagtattt gccctgttca tggatatata cctgggaatc acacatattg
     5221 tccttatcca catacaaaag aagagcttga gatgtttgga atagaagtcg aaatagaaga
     5281 tagagaatta tcaaaaccca tagagacaac ccatcggatt gtctcgtaaa cgtataagtt
     5341 gttgaataga ttgtatttat gagtattgct tgactgtata tttattttca agggattgtt
     5401 gatggctgca ttgcagttat ggtaagtgat aaaaatggag tactattgtc attctgaaca
     5461 aagtgaagaa tcttttcgtt ttatggaaga atagattctc tgggagcgcg tctctcagaa
     5521 tgatagtgtg cagtgtgtta tcctgaataa tattcttaac tggtttcagg atcatttaag
     5581 gatataaggt tacaaatttg gattcctgaa aaggtcagga ttgacaaaag cggcaaaaga
     5641 gattcttgtt caagtacaga atgatattgg cacataaaac tatattcgga gggggttctg
     5701 atgcaaagaa atactgaaaa gaaaatacct gttgaggtat attctcgtgt tgttggttat
     5761 ttccgacctg tgaatcaatg gaataaaggc aagcaggaag agtttcatga aagaaaggaa
     5821 ttcatggcaa aagaattatt aaaggttgta gatcatgaat attcggggta tacagaaaac
     5881 atcactcatt gattatccgg gtgctattag tacagtactt tttaccgggg gatgtaattt
     5941 gcggtgtagt tattgtcata atccatcatt agcatataat gattgtaacc tacactatta
     6001 ttcaaatgaa gaagtaattg atcttttacg aagtagaaaa catctcattg gagcggtagt
     6061 actgacaggt ggtgaaccaa cgattgcaaa agggatactg caatttcttg aaaatattaa
     6121 atcgctggga atgaaaataa aattagatac caacggtttc cagcctgata ttctaaaaaa
     6181 aattataata aaaaatcttg ttgattatgt tgcaatagat ataaagacat ctcctcaaaa
     6241 atattcgctt ttaacaaaaa caaagttatc gtttaattgt atcaaagaat ctattcgttt
     6301 gcttaaagat tttgatatcc aatttgaggt gcgcactacc tgtgtgccag aatttgtaac
     6361 attagaagat ttacatagca ttaaagaaca gattggtcag gtatcccatt attatttaca
     6421 gcaatttaac aatgatcaca caatggatga agacttacac agagtaaaac cttatccaga
     6481 agaaatatta ttattgttcc gccaatttgt ccaaaccttt gccacttact gtgacatcag
     6541 agggctatag aaaatctgtt gacattattg tatgatgttt ttctgtttca tgtaatctgg
     6601 catttagcaa gtaattgcga ttttttttat caattgggca agttaaatat aatactataa
     6661 taattactca ttataaacaa taattgtcac tataaaatgg gtatatcaag cttaaaaatt
     6721 ttgctatagt ataaagaaaa gactcattat catgatacaa tatacaatcg aagtaaagaa
     6781 ataaatttgt tgttaaaaat tgtatttccc tgataatggc tacattaatt tttttaaagg
     6841 agacctgaaa atctatgagc aagcagtata atccaaggat taaacgaaaa cgagctaaac
     6901 gaagactcaa aagaaaaaag aaactaatgg caaagaagat agggcgataa atttagttgc
     6961 tctgtttgct gataatctca ccacccacaa taagtaaaat atttgatata ttgagtactt
     7021 ctgatgaggg gtatgagttt ttataagtta accatatccc cttatcaggg gttaatggta
     7081 tatacagaaa cactgtatca aaataaagat tatttttttt agcaacaatc tttttgtgtg
     7141 aatcccccag cgcaatgcag gatataatac cttttttagc taataggtat cgtgcgtaga
     7201 gcatagcatc aagctgtgtt cctttatgca gaagagtcca ttgtactata gaagtgatac
     7261 cataaacatt atcggcaata gtataggttt gtagttcctg gagcacctcg tgtattgcct
     7321 catctattga gttgccctga atttctttta tgtctaagag ttcaatgccg cgtttaaaca
     7381 gggcattctg ttcttctatc catgtaatta atgcttgtgg ttggatagcc atgataacaa
     7441 attccctatt gtttggcgat aacaattcat tactattgca ggtatagtga tgaatatacc
     7501 taacattggt tgttatttca ttgaggcgca ttgttcccga tatggttaca tctgattcag
     7561 gagataaaac gataatatcc agcgtaaatg gtgattgctt gtaattttgt tgtatatgca
     7621 ttatatcgat gttttttcgc aatcgaaatg atatcgctat tacaaaaaat tcacagtgac
     7681 tatcgtgtaa atattcctgt atcttctgtt gtattgtata gttgccggga taaaaggtgt
     7741 atgatttcat tttcccttcg ttattgtatg catagagagt ataactaata ttctcagtgt
     7801 cgggagaaaa ttggtaggta ttgaatacgg atatatcagt acaatggtat gcgtcgtata
     7861 tggtacaata tagtgcatca tgaaccatcc gcactgcata acatgaatac attaatttgt
     7921 ttgtggtatg attgataatt tcataagcaa gctgtttaaa aaaataattt tgcacgtaat
     7981 catcaaaagt ggctttcata taatcgggat gcacaacaag actattgaca atcgcatgat
     8041 gcttttgact atacatgctg gcgtcatagc cttctaattg agctattgtg gctaatagcg
     8101 cataagcttc agtgtcaaaa ccctgtgcaa ttgttttaaa caaagcgctc tttgcttcac
     8161 catacttacc aagtgcaatg agggcattga tataatcata aaaaaatgta tgttccctga
     8221 caccagaaag gatagttact attttttcaa attgtttatt ttcagtatag cagtgaatca
     8281 gcaatgtaag ctccttatca ttgaggtttt tatatgtttc caatattgtt gtcgccttgt
     8341 gattatcata ggatgaatag atagatgcta tggtaaaggc aagttcatgg ctttgcggaa
     8401 agcgtttgaa caaaaaatct aaatctgctt ttttattaat gagttcttct ttttggatgt
     8461 ataaagtttg tataagtatg ggtagccact gtatttctgc aatattcatg gactctggtg
     8521 aatagagcat atcgtaaaaa tagccaccca gcactgtttg aggttgtata tttttttgca
     8581 cgttagttct caacaacaaa atgctatccg ggaatgcacg gctatacctc agtagcaatg
     8641 aacacaatcg gtatgagata aaatcgttgt gtgaacgcaa tgcttgatga taatatttga
     8701 aagcctctgt gctgtgaatt gattctgaaa aaagagcaag ttgcagtgca ttatcaggcg
     8761 aatggttgct catatagttt gacagaagtt gattttgata ttcatagaat atctcataac
     8821 agttaacagg attataatac atggtgtcgg tagcaggtaa ctgcatcgtt gcattggttt
     8881 catcacgaaa cagtattttt aagtacatat cattgtctgg tttgtaatat attgctatgc
     8941 tgaatccctc ctgtggttca atatgaatga ttcgagcatc tttttttact gttgttgtcg
     9001 tattaacaca tacttttgtt ccattgataa aaatggcata ctccatattg gaaaaaacat
     9061 acatagaaac cggtttatgt aattcaaacg atgcgctgca tagtcctgtt gcgtgtaaaa
     9121 tctgggtgta ttgctttaca ggtatatacc ccgcgcatga ttgcattact gctattttgt
     9181 ttttatcagg aagtattagg gtatccagtt gtgtttggaa agatatcacg cttttgggca
     9241 ttggttcctt actattatca tagatatacc agcgttgatt tccttttaca tcgtttttat
     9301 agaaacgttc ttttgatata tctgcaatat cgttgataaa cattttttcg tatggtgatt
     9361 gtgcattggt agaaagcaat tccagcagtt ctattgtttt ttcaattcca attatctgct
     9421 gtatatgttc actatccgct aaaatgagca ggtttggtat tgtaaggttt tgcgcaccgt
     9481 ttacatactg ttcagcgagt gcttgtgaat ctccactaca atacatagta acggtatcat
     9541 gacctatgat atctgaagat gcatcaacag taactatcgc cactatatat aaacttaaca
     9601 ctatagttat acaatatttt accattacca tgccatcatt tgcctataca aaagcgtgaa
     9661 aagattgaat tgagaacatc atcaacagta atttcaccgg ttattgcacc aatagtgtca
     9721 atgacatcct gcaatgctga tgctataatc tcgtgagggc tttgcttgag tagctgctcc
     9781 cgcacctcat gggcacctgc gctggattgc tctaaaagct gtataatacg aatatcagca
     9841 ataaatgaat tggtgtagtt gacatacgag ctttgaataa aatcagacac cgtatgctcc
     9901 agcacatcca taccatagcc tgttgtggct gcaaacacaa tccctttttt gggcaattgt
     9961 gtattgatag cattgatttg ttcgggtgag gcaatatcgc atttattaat aacggggata
    10021 tatggtttgg aactgatgcg ctcaagaata tgggaatctt caacggtaaa accacttgta
    10081 gcatcaagga taaaaaataa gagtgaaacc tcgtcaataa tcctgtagct tttttgaact
    10141 cccagcagtt caatccctga tgctacatca tgaatccctg ctgtgtcgta tagattacat
    10201 tgaatgccat gaatggtaac tgattctttt ataacatcac gcgttgtacc cggttcatct
    10261 gaaacaattg cacgctcatg attgaccaca cagtttaaaa agcttgattt ccctgcattg
    10321 ggtttgccaa caatagcaat atctatccca tgagataaat tattcccaac cttacatctg
    10381 ttgataattt cctgtatggt atgaataata tcttctatcc tttgctgagc atcctgataa
    10441 gagattattt cgatatcctg atcaataaag tcaatggcac attcaatatc agcttttaac
    10501 tctactattg tttgtttcag tttattgata gtagtgcgta acgatccatg tttttgctgt
    10561 attgccgtag ctatttccca ttcactccgg gctgttataa tatgattgat agcttcagct
    10621 tcggtgaggt ccatttttcc gttaaaaaaa gctcgtttag taaattctcc tggctgggct
    10681 atacgtacat gcaatgccag aaggcgattc attattttat taacaataat aggattgcca
    10741 tgacagaata tttcaaccat gtcctcgcct gtatagctat gcggttgtgc gtaataaata
    10801 actatcgcat catcaatttc atattcccca tcaaagattg tcccatagta tgcataacgg
    10861 ggtgtggata tagtttgttt attgtgttgt ttaaaaatag aagaagcgac ccgtaaggat
    10921 tcagggccgc tgattcgtat aattgctatt ggtgcatgta caggcggtgt tgctggagca
    10981 caaatggttt cttgcatctt acctgccttt ttcaatgggc ataattttta cctttcgata
    11041 tatgccctgt ccctcacttt ttgttgtcac ttttgagtca ttttgtaatg ttaaatgaat
    11101 aagccttcgt tcaaacggat tcatgggttc aagtgtccat ggttttccgg ttcgggctac
    11161 ttttaaagct atctccttcg ataattttcg taaggctcgt tctctttttg ctcggtacga
    11221 ttctatgtca aggataatct ttttttctga actggtaacc ttattaacca gaagatttat
    11281 catgaattgc aatgcttcca gtgttttacc ttttttacct attattaaac ctgaatttaa
    11341 actttctaac tctacatata ccttattttc gctttcttta aaatctttaa cagtagcatt
    11401 aacatccatt aatgacatta ttttttctat tatttctttt gctttttcac caacatcatt
    11461 ttgctgctga tagtatactc taatcttagc agggcgtgat atgccaaaac caaatattcc
    11521 gcttttacct tcatcaagga cttcaatgtt gattttgttc atatcatcaa tttttaattg
    11581 taataatgct ttttttgttg cttcttcaac ggtttttcct tcaatatcta aaaccttcat
    11641 ctccacgcct cctatgatac ttataataat gaattatgat aatagtattt gttacacacc
    11701 tcctgaaaaa atcaaaaaat ggtagaaatc tcatattgta tttaatgtac tataattttt
    11761 gcattaagac ttacatttat cctcgtttcc atgttgctac ttttcagtct ttggtttccg
    11821 gtttactacc atttgatgag ctatttgtaa aacattctgc atgatccagt ataataccaa
    11881 tccggatggc atattccaga atataactat aaatataagt ggcataaaca aaagcatttt
    11941 ttgctgctga gaagcttcgc cagtagacat tttttgttgt aaatatgttg ttgctgtcat
    12001 cactataggt aaaatattaa tgttgaatcc tgaaatggtg aaaacagtat caggtaatga
    12061 aagatcctta atccacaaaa taaatggtgc ctgccataaa tctacagaat taatcagagc
    12121 gctataaagt gcaaagaaaa atggcatttg caacaatagt ggtaagcacc cacccatggg
    12181 gttgaccttg ttctttttat atacttccat tatcttctta ttcaagatat caggcttatc
    12241 tttatacttt tcgcgaagtt cttgaacaac tgggtttaat tcctgcatct tcttcattga
    12301 ctcagtagaa cgtttggtta agggtaagaa cattattttt gttataactg aaaatattac
    12361 taatgaccag cctaaattcc ctaacaattt attaatgcca agcaaacacc acatcataaa
    12421 atcacgtata ggctctatcc atttgcttac atcggctgca tctccaatct gtttatccac
    12481 agctaaaagt ttatccttat ttttaggccc aacatatatc ttgaaaacgt gttctatttt
    12541 ttcgccaggc tttagaggtt ttgccggtac aataatcccc gtcctgaatc cagtatgttc
    12601 cctgttatcg taaacaacac cagaaccctg aaaaccttgc ggtatcatta tgagcagata
    12661 gtacctgctc atgatgcctg cccattttac aacaccattg atctctttta tatcctcatg
    12721 gtttgaaaaa aatcctgagc ctttccgtgc cttttcaaaa tcatcatcaa gataatatat
    12781 actgttcaac tcgttgtacg aattattaaa atccattgag gggcctaaaa aatctgaagg
    12841 agaataaatt aatctgcctt caggaaaaga aaattcattt atagctctat tggtgatact
    12901 gaatgacacc gtaaaaaatt gccctttttc atcaaaggta aatgtcttta caatgtgcaa
    12961 tggattattt tgcacgatag cacctgtaaa aaagcgtatc gaatctgttt ctttttgtaa
    13021 tttccagttt gtataatcac tttctgttga tagcaaaaat tctccagttg taaaatgtac
    13081 aggaaaatca aaaatacccc tggcaatgta tttgctttta ttgaccacaa gctcaatatt
    13141 tcgggtttta tcaatgaacg aggtacatgc agcgccatga ttggaaagca cggctttata
    13201 ataatgtgaa tcaacagtca cttcttcctt taagcgggat aattccagga attttgaaag
    13261 ttgtttcaat tcaggttttt taatttcaac cgtttgttca acttcaactt tcttatcttg
    13321 aactgcaggt tttttttcct gcggcggaaa taaataaaac cataatatcc atatcagcaa
    13381 agataataaa actgcaatta aagccctttt ttccataaca acctcttgtt taactttcta
    13441 gatataaaat aaaaaaatat gactattatt gaaatattat aataggtgaa aaggagtgat
    13501 atgaggggta actgtcaaga attgtgctaa ggaacaggat catacccacc gtcacaatat
    13561 ggatgacagc gtagtatccg cttgattgat aaaaaaccac ctttacagag tccatgtttg
    13621 ctcaatgctt ctattgcata gcttgaacat gttggataaa atctgcaaac attgggtaac
    13681 aatggtgaaa taataagctt atacattttt atcaataata ttggcaatga aataatattt
    13741 atcttcattg tttgagcact cctgatctaa gcaacaattg ttttaccaaa cctgaaaggt
    13801 gagtatatgg cattgcctta caggttttat caggaaaaat aataacagca aaaccctgtt
    13861 gtatctgagg caaaagagta ttaagtatag attttattct tcttctgatt ttgttacgtt
    13921 caactgcatc cccaaaattt ttattaacag ctatggaaaa atgtgaaact gactcattcc
    13981 caataaacaa tgtttcatgt gctggttgta tatgcactat cattcgtata cctttactat
    14041 aaaatcgtct gccattaaca ataacatctt taaaactcct ccgccctttt attgaatatc
    14101 tccttttcac cggaaaataa ttatacaaat atatgctact ttacattacg tttttcatct
    14161 gacacagtta gtttacaacg tcccttctgt cttcttcttt tcagtatttc tcttccagct
    14221 ttggtactca tcctttctct aaatccatgt gttcgtaatc gcttgattac acttggttga
    14281 aatgttcttt tcattattcc acctcattat tatgaaaata gtacgataca gtttcgcagt
    14341 taaatattcg ataagtaagt attttattaa taattaatat gttaaattga taatatcaat
    14401 tatagctttt taataatagc acaaatttgt aaataaacat aacaaaatta caattttatt
    14461 ttttagaatg tctatgtcaa gaatttatta tattactttt ttgctattac catgatagta
    14521 tctgaaaaac aaatactatc agatagtttt atatatattt ttttatgttt tgatgccaaa
    14581 gagcaaaacc gttcaggatg atatccagaa ggatagcact ttacttttac aaaacccatg
    14641 ttttttaata atcgtgtaat accacgtggt gatacatcaa ttctatggtc tggggggtga
    14701 tttttaatcc agatatctct gtgacaataa aacaatggtc caaaatatga tggtaacgtg
    14761 catgcaaaaa taccatcatt atgtaacatc gaatatactt tttgaaacat tgcaaaagga
    14821 ttagcaaaat gctccataac ataccacgcc gttataacat catatttttt agctgtcagg
    14881 aacttttcaa aatcaactgt agtaactgat atggaaaact tttccttaca atattgtgct
    14941 gcatattctg atatttctat tccttcaacg aattgaaaac cactgtcttt agctgcctta
    15001 caaaaaaaac ctaatgcgca tcctatatca agaagactct tttcggacag attattattc
    15061 agtaattttt ttattttttc tattctcagt aatgaagcag tgtatattgc gtcatgatca
    15121 tcttcgtatg tttttccgta ctgattcttg tagtcttcac caaaataatc agcagtataa
    15181 ggtgatggtt gatatgcatt gttaaacaat attttacatg gtttgcataa aaaaatatcg
    15241 tttgcaattt gttttgatcg tgagtccttg cagattggac atagtaaagg tatcatttat
    15301 ctcattgttt tttctttttt gctggttcta ctacgatagg aataccttta atccttgctt
    15361 tatcaagtga tactaataca agttcagcat attgttctgg tatctcaata aacgaataag
    15421 tggaatgtat atcaatttta ccaaccagtt ttccatcaat acccgaacgt tttgatatct
    15481 cacggataag atcaccaggg gtgattttat gattcttacc aatatttata aatatacgtg
    15541 cttttccatt tttaatatct tcaaagacaa ttgttccgtc agattttgcc atgtttaatg
    15601 acgttatttc agataaatcc agttttgaga tatccccgat gcactctttc aaaagcgctg
    15661 cagtaatcct ttttgttgaa ccaaataagg gtaaatgttt tctcatagct ttaatgagca
    15721 aaaaatatga agttaaatcc tcattcaaag cagattctaa aatcttttca atctttttaa
    15781 cctgattcat aattactcct gaaaaaactg attacaaaaa cttaataaag tttttttaca
    15841 ttaacgaaaa aaacatgtag attggcaagc atatttcttt catcaatcct tacacttata
    15901 gccaacattt ctcactgttt caataatatt attgaattca tcgcctattt ttgaccgcaa
    15961 acggcggatg tgaacatcaa cagtgcgtgt accgccaata tactctatac cccagatacg
    16021 tgataataat tcctgacgtg taaaaactac tcctttattt tcaataaaaa gttttaacaa
    16081 ttcaaactct ttaaatgtta atgaaacttt ttcattgtta accgatacaa tgtatttgtc
    16141 aggttcaata gctatatttc caactctaat gactgcatct tttatttcaa tttcagagcc
    16201 tattaaacag tctatcctga ccttaagctc accttttctg aaagggaaga tgataaaatc
    16261 atcaaaaaac caatccctgt ttatagactc aaggtatcct gtacgaacga tgagaataac
    16321 cggtacatga gaaaaacgtt ttcgtatttt tgataaataa aattgtgcat cacttatgtc
    16381 gatctgctca ctatcaaaaa gtattatatc aaatccttca tctttaagag tattaattga
    16441 accgtgaatg cttgcatcca ggggttgtac cagtcgtact tctgaaacca gttctttact
    16501 ccgggtaata agtgctgttt taattttcat tcatatccac taattgataa aaaatatcca
    16561 tatattgctt ggctacattt tcggcataat aagctgtaat tctggcttta ttattttttg
    16621 aacattgagc atagagcttt ttatcatttt taaatcgcat tatacattct gcacattttt
    16681 gtatgtcgcc tacatcaatc agcaaggcat tgtgttcatt ttctaaaaaa tcaacctgcc
    16741 caccggaatt agtggtaaca attggcagtc cacaatacat cgcttccata aaaacaatcc
    16801 caaacccctc gtgcaatgat gttaaaacaa aaaggtcgga tactgataag tatgcatatt
    16861 ttttatcatc agatacaaac cctaaaaatg taacaatgtt ttcaagtgcc aattcttgga
    16921 ccagttgttc aagatactca cgttcgggtc catcacccat gataatcaat tttacatgtt
    16981 gattattaat ccgggctatt gctttaatca acacatcaat tgcttttcgt ttaatgagcc
    17041 ttccaattgt tatacagata aaatcttttt catgcagccc taacaatttc cttgagctct
    17101 ttggtataac aggaggatga aaagcaagag gtattatgcc tatgtccttt ttgattttat
    17161 agtagtggaa ggcgttgttt cgagtatttg aggattgtgc tatgatagca tcagccttgt
    17221 taagaagata ttggactatt aatttgaaga aacgattgcg atgtggtgaa aattttttac
    17281 tggggtcata aatatcacca ccatgaagcg ataagacatt tgggatttta aatagctttg
    17341 atagcacata tcccaatggt cctgatggaa caacaaaatg tgtattgata atgtcaaatt
    17401 tgtatttctt aaagagcgac agtgctctga taaaacctgc tggtaaatag ctaaacatcg
    17461 agataaatgt tgctgcatca cgagactttc taaataaaat cttaactcta tggacatgta
    17521 tcccattgat atattcatat gggggtaaac ctttgaaaga ggaagttatg atatgtacat
    17581 tggcaacttt tacccactct aaggctaaat cataggttgc aacacccccg ccaccaccta
    17641 acggtgggaa ttcataattt ataattaaga tattgatttt tttgtgtaat tccatgtgga
    17701 ttatcctatg gtattgtaaa cttttttcat ttattgctgg aattgaataa tacttcattg
    17761 cacttacgct attattgtat tttcttcaat aattttttta ccaacattgc aatattttta
    17821 aagaactatt tgttataact atgcgatgaa ataaaataat tgattgacac attgtacagt
    17881 ttgtttatgg tcaataagaa aataacagaa tgaattaata taataaaaaa gtatagcaaa
    17941 ggaatatggt ataacaatat acacacaagg tggaatacaa catatagtgt tcaatagagt
    18001 gcttctatga gatagttgtc gatggactac ccgtagggga tgaaaattgc atagtatcgt
    18061 catgctgagg cgaaaccgat agtcattctg aacgaagtga agaatctcgt cattttataa
    18121 acgaatagat tcttcggggc tgcgttcctc agaataacaa tgtggggtgt gtcatcctga
    18181 ataatgttct gaaccggttt cagtatcatt tcaggctcta agattacata tttaaattcc
    18241 gtggcaagcc cggaatgacg aatgtggaaa aagagattcc ggacaaagtc tgggatgaca
    18301 ttggcacata cttctatttt cggaggagcc atatgctctt ttgggtgggg aagaaacatt
    18361 gagctgttgt tatacaaata tgataaacga tagggtaatg ttatttcgag gataaaaact
    18421 atcatggcaa tcatgttgat agagaaaaga tatttttgcc aaaatgtaat aggttgtgca
    18481 atgactatga atagataaat gcaatgtgaa gatgcatcac tccgatacaa acacactgag
    18541 ggatggcata gaccacgata ttgtaatttt tttggaggag ggttgttgtg agtataaaag
    18601 atatactgat ttatttttat ttacccgtta tagtatttac tgtattatgt ttttgtgcaa
    18661 ttgttgtagg tattatttac tggataaaaa aatataaaga acttttaata attctttttt
    18721 attttttaat ccatgcagga atgactgcaa tgtatattta tccttttaat gtatttgcgt
    18781 ttagtttgca atatgcgttc tttttatttt gtctgttgat gttttgtttt agtgcagttt
    18841 tcatggtgtt accagatgca tcaaataatt cagattcatc ctatagagca gtgcaatgta
    18901 ttgttgggaa aagtattctt gtttatgtca gtatagtgtt tttgacgcca aaagagcaat
    18961 taataataag cactgttgat tgtatggtat ccttagtatt tatatcagtg aaacctaaac
    19021 ctgcaacaag ctgtgtgcaa ccatttgcag aaaaggaaaa atttattttt ataatcgtga
    19081 ttacaattgt ttttttgtgg ataagtatag caaaaatatt tttatttcat gttttaatat
    19141 tgggtatgac tatccttgtc attgtcaatg ttgttttagt atgtgtcata gcattgaagg
    19201 tatatgtcta taaagacatg gattttattg caattcagca ggaaaattat tgtggtatgt
    19261 atgaaaaaca ttttaaaatt gttcagataa ttaaagaaca tattaatgaa tactacaccc
    19321 acccaattga taaatataca atagcactcc aatgtgagat gaatcctgat tatctttgca
    19381 agatttttaa aagcaatgaa ggaattacca tcaatgaata tataaaaaat atacggttaa
    19441 agaaagcatt ggaaacattg tcaaatactg ataggaaaat aattgaaatt gctttagatg
    19501 taggttatgc caatttagca actttttaca ggcattttac acagttttat aaaatatcac
    19561 caaatcagat aaggataaaa aaaagtaata atataacaac tgtttaagac taacaggtac
    19621 attccacaat aactatcgca caaataaaca tgcatcattg taaaggctgt tcaaagatta
    19681 ttcagggcaa gctaaaaagt tttttaaata tttttttgtt gacttgaaaa tgtaaatagt
    19741 actattattt actgtttatt aagtatgcaa tttaaataat tcatactaca aaagtaaaag
    19801 gtgaacagta tgattgaatt aacagataaa gggaatggta catttgttgt aaagattaat
    19861 atgcaagagg ttcataccct tgatgttccc gaccttaaag aaaaattgca acaagctatt
    19921 gtgaataaag ggataaaacg gttggttgtt gatcttgcag atgttaaaat gataacgagt
    19981 tcggggatag gtatattttt aaatattaat cagaatttga aatcacaact acgcttagca
    20041 gcaccaacac cggaagtaca aaaggttctg gaacttacta aagtaacatc aatgataaaa
    20101 gtttttaatt ccgtagatgc tgccctgcaa agtttttaat gcaaaactat caacaaaaaa
    20161 attatttacc ctgcttcatt tatgtgctat acttttattt aattatcaat tgtagttttt
    20221 atagtattaa tcgttgtaat acattgtata tagcacgatt ataaagaata gtaatcgtac
    20281 tatcttatca agagtggtag agggactggc cctgtgaaac cacggcaacc ggaacgaaaa
    20341 gccggtgcca attccagcgg aagatattcc gggagatgag atagcaataa gtatccgctt
    20401 acacgatctt ccggtaagcg tttttttatt ttaaggggga gttgattatt tacttagatg
    20461 aaagcggaga taatacctgg aaattcggct ggtatcgtta ctactaatta tttcactttt
    20521 gctgagccac ctgatgaatt gttattggtt tcagggaaaa aacttgggcc aataacatta
    20581 gcctatgaaa catacggtac tcttaacagt gacaaaagta atgccatatt aatacttcat
    20641 gccctttcag gttcagcaca tgcggcagga taccattcaa aaaaagatat atatccgggt
    20701 tggtgggata tttatattgg tcccggtaaa acttttgata catcacacta ttttgttatt
    20761 tgttccaatg ttattggcgg atgcagcggt tcaaccggtc catcatccat taatcctgaa
    20821 acgggaaaag aatatggttt ggattttccc attgtcacta ttcaggatat ggtaaatgcg
    20881 caatatcatt taattaaatt tttggggata caaaagcttt tatcagttgt tggaggttca
    20941 atgggtggga tgcaggcatt gcaatggtca atatcatatc ctgagatggt gcagtctgta
    21001 gtagcaattg caacggcagc gtctctttcg gcacagggaa tagcattcaa tgaagttggc
    21061 agacaggcaa ttatgcttga tcccaactgg aattatggta aatattataa caatgaaaat
    21121 cctaagaatg gactatcact tgcgcgtatg attggacata taacctacct gagcgagaag
    21181 ctgatgcacg aaaaatttgg cagaagattg caacattcag atatttttaa atttcacctg
    21241 gatcttgagt ttgaagttga gtcatacctt caccataaag ggaatgattt tgttcagaga
    21301 tttgatgcta actcatatct gtatatcaca aaagctattg attattttga tttgaagaat
    21361 gattttaatg gtgatctggc taatgctttt gttaatgtaa attctaatta ccttgttata
    21421 agtttttctt ctgactggtt atacccaact tcacaatcgc gcgagattgt tagagcgttg
    21481 cgtatgaaca gcaaaaatgt tatttttacc gaaatacaga gtgataaagg gcatgataca
    21541 tttctactgc cacatgaaca attggagcat aatatagcta attttttaaa acgggagttt
    21601 gaaaaggttc accggtaatg aaggaataca gggttgcata tgatttgatt ctaaaagaga
    21661 ttccatatgg tgcacgggtg cttgatttag gatgtggtga tggtcagttg ttgaagttgc
    21721 tacaaactac aagaaaagtt aaaggatttg gtgttgaaat ctcaccggaa ggtgtaagcc
    21781 aatgtgttga aaaaggattg tactgttatc agggcgacat cgatgaaggg ttaaacgatt
    21841 ataaagacaa ttcatttgat tatgtgattg ttaatcagac gttgcaaagt acaaaaaaac
    21901 cagagattgt tatacgcgaa tcgcttcgta tatcattaaa tgctatcttc agttttccta
    21961 actttgccca ttatagcctg aggttgtata tttcaatcta tggccgaatg ccgaaaaata
    22021 aattattacc ctatgaatgg tatgaaacac cgaatataca tcttttaaca attaaggact
    22081 tcagagattt ttgcaatgcg tttggttatc ctataaaaaa agaaatgcat ttttcaacca
    22141 ttcgttctca ttcagtaaca atacctatta tgcccaacct ttttgccgag tatggttttt
    22201 ttgtattgga tggaggacca tttacctctg atactatatg attccatccc gttgggataa
    22261 ggtattaagt tttttctggt gagtagtgca aaaaaaaata aaacaaaaat tttggctaca
    22321 cccaacccaa tgtttttctt ttttaaaaga taataatata tccttcaaag gtattccgct
    22381 acatcataat cgttttactg atagagtaat tactcttaca attaccagtt agtgtagcca
    22441 aaataaacta taatgtattg atgcagtttt ttattatcta atagtaaact tttacgataa
    22501 gaaattacta ttcattttat gtcaaatatt tttttatatt aaaataaaaa aatatattat
    22561 ttatataata tgttgttata atatataaca aataattata tttaatcctt ctatttattg
    22621 aaactattaa aaacattgtg ttgaaattgt tgcaatggct ttcattgtat caaaaagcat
    22681 ggtaaaccca atagtgataa attcaggtta atttgttgat aaaattattg atttcaaata
    22741 acgtataata atagttgtat atgttcaacg gatgcattgg atgcatttat agttttataa
    22801 attatgtaat aaagaattgc aggcgatagt tacttgaaga tttcattaca ccttattgat
    22861 ccatttcaat gaagattcaa tttgtacaac ttccgctgat tgatcatgga ttacagtaca
    22921 ttaccggtaa tatccccaat gctgctgcaa ccctgcacgc cttctgtgag ctaacctctg
    22981 gtggtaccat gaaaggatta caactgccgt atagcatcca gaactatggc tctgattcga
    23041 tgattgtgaa tcatattttg cacaatgagc caacgcatgt tgcattcact tgctatttgt
    23101 ggaatgtaga gcgttcactg gcaattgcta aaacgattaa ggctatggca tcacatatca
    23161 taatactctt tggtggtcca gaaatacagg taaactcata tatcctgaaa cagcaccaac
    23221 cgtgcataga tatatttgtc attggcgaag gcgaatattt tttcaatcat tactataaca
    23281 atacctgcaa acaatttatt catatggtca atggtaacgc cgtttttatt cagggctctg
    23341 acacctttat tccgctgtca cagatagtgg aaccctatac atctggatac ctcacaccaa
    23401 tgcatgataa aagcctgagt gttgaaatta ttcgggggtg tcctttcaaa tgcacctatt
    23461 gtctctattc taaaaatacc agcagggtca gagccttacc gccgcacaag cttgccgata
    23521 ttatcaaaaa aagtatgagt gatgaaatac aggaactgta tcttttagcc cctacattta
    23581 atcaatcatc acacttcaat gattatctgc aaacactgat atccagcaga tgctctgtct
    23641 cactgcatac tgagctcagg gccgatacca ttaccagcaa cattgctatg accttaaaga
    23701 aagccaattt ccgtagcgct gaggtaggca ttcaaacatt aaataaaaaa tgtttacaaa
    23761 agattggcag aaaaacaaac ccggaaaaag agctacaggg gatattctat ttaaaagatg
    23821 caggcatcaa tttacaggtt ggcataatcc ccgggcttcc ctatgatacc cctgaatcat
    23881 tttatacaac aatggacact ctgcttgatg cagggcttgg gaatgaactg gaactttacc
    23941 cactgatggt gctgccgggt accgctctgt atgatgaggc aattgctgca ggtgcaacat
    24001 ttttgcaaaa gccaccttat tacctcattg aagggttcaa ttttacgcag catgatataa
    24061 cagccatcac atgttatttt gaagacacca cagggtttac ctttgatacc ccatacctgc
    24121 catcgttcat ttttcagcag ggggagcttg tatcgggagc ctgcattgac cttacaaagc
    24181 gccgttccat ccctatggaa gtatttgatt atatgcaaac aagtcatatt accttttatt
    24241 tgctatgcga tagttacccg caaattgaac atgccgtaga attaataatc cagcagtatc
    24301 catgggacac aatgctctgc tcaattgtcc ttatgtatga tgcagttctt aacgatgagg
    24361 atattgccag caaagtattt acagcaacac ataatacctt ttattgcagg atgatgtatt
    24421 ttcaggatac ctatgataag ctgcctgtgc gtatcttcca ggtatttgaa tcattgaaaa
    24481 catatctcag agctaaagac cattaccgct gtatcaaccc tatcattaaa ctgttgcgca
    24541 gcaattatga tgaaattaca cagtatcact ttgcctatcc tgtgccggct gttgtggcaa
    24601 aaggttgtgc agggatatat ggccaatgga taaaagaaca gtacgctgaa tgtattgata
    24661 ttgtaagctt tgaggatgat gatgatcaaa atatgatata taaaactctg gggattgacg
    24721 gtaaaccgac aatccccttt acaacactct ctatttaaag catctctttt aacggcaata
    24781 caaaaatgct aacgccagca tctttaaatt taacctttaa agatgctata tcggacgcaa
    24841 tagcatcttt cattttatta tcttctattg ctaccatcaa acagttgtta agccctcccc
    24901 atatatgcgt ccctaattgt ggctctttat tcccttcacc cagtacacct acaaactttg
    24961 tatacccttt tgcatacttt tttgttatct caacaacctc ttcatcaaca gcattgtgat
    25021 atacaataaa taacatcacc atggtaattc ccccttaaaa ctttgcttta tgttttgccg
    25081 aatacttttc acgcctggtt tcaacgatca tgtatataac aggaataaat ataagtgtaa
    25141 aaataaatcc cgtactcatg ccacccataa cggctctgcc tattggttgt gacatttcag
    25201 atccatcacc gccactgaat gccataggga ttagcgaaag tacagttgtg agggtagtca
    25261 tcataattgg cctgagtcgt tttgttccgg cttcaaccag cgcttcgcgt aaaccccatc
    25321 cccgttgtat taaaatatca gtataatcca caagaactat cgcattattg accactatac
    25381 ctaccagcat aatgatacct aaaagtgtag caacactgaa ctgtgaacct gttaaaaaca
    25441 tgaataatga aacaccaaac atacaggtag gaactgaaaa cattacgata aatggcctga
    25501 tcagtgattc aaactgtgct gccataataa cataggttag tatgccaccc agaattaata
    25561 ccagcagcag atcattaaag gtatcctgca tatcctcata cgatccacca attgatacat
    25621 aaaaattttg tggttttggt atttttgtga gcgcttcgtt gatgtctgca gcaacagtgc
    25681 tgagagctct tccttcagcc ttacagttaa tgtatactac ccgttcgtga ttctttctgt
    25741 ctatagcagt tggccctgtt ttgtcaataa catcaattat gctccctatc tttattcgtt
    25801 ttcccatagg agtgtcaatt tccaatgact taatctgttc aaccgtttgc ctatcagcct
    25861 cgcgcaatcg cacattgatg tcgtactcat caccctcaaa acgatagcga gaggctacaa
    25921 aaccagaaac attgtccttg ataagattgg caacctgata ggcattgatg cccatctttg
    25981 atgccttttc cctgttgacc accactacct tttcgggtaa gccttcttca cgggatatat
    26041 ctatatcttt taaaccaggt atgtttttaa tactgtttgt aatactctga acatattgat
    26101 tggattgttc aaagtcataa ccatatacct caatggagat ggtggctcct tcagaaccca
    26161 ttcctattga acttgatccc ctgctctggt aggtaaagtt ataggtaacc cctgggattc
    26221 cttgtaatgc ctttcgcaaa atatcacgta tttcagcctt tgatcgttcg cggtattttc
    26281 gcatctttaa ttctatctgg atccttgctt tatagtcagt taaatcatta aatgctgcat
    26341 tggttccctt tccatatcct gccctaacca tgactacctt taattcttcc tctttaaata
    26401 ttggcaatgt gcgctcttct attagtttca taattctagt agtttcatca agattggtcc
    26461 ctataggaag ggttgccatt atggttattc tggaatcgtc gcgttcaggc ataaactcca
    26521 tccccgacag tggtatcaat attaaaccaa taaggaaggt tatgccaaca aagccaataa
    26581 cctttttttt attatccagt gcccagttta ttgctttgcc ataaaagttc tctaaatcaa
    26641 tgaagaattt ttcactttta tcaaatagag gctgtagaac atttgatctg ccggtatagt
    26701 gatgctctat tgtaggtaca aactttgcaa tgagcatggg gataagggtt aaagcaacaa
    26761 acaatgaact catcattgaa aatgtaatgg tcaatgccat atcggtaaac aactgagcgg
    26821 ctaaccctgc actaaacata aatggcacaa ataccacaag gttggtaagg gtagaggctg
    26881 tgattgctcc ccccatctca tccgttccaa gttttgatgc ctcattaagg cgcgcccctt
    26941 tttcacgata tcgaaaaata ttatcggtta caactatagc attgtcaata accataccaa
    27001 caccaattgc caacccgccc agtgacatca tgttgagaga tagatcaaat aaatacatca
    27061 aaacaaatga ggtgataacg gatattggta ttgataaccc tactaaaagt gatgccctga
    27121 tattacgcaa tataagcaga acaacagcaa tagatataat acttccttga ataagattat
    27181 ttaaaaggtt ttgtattgag aactgtatca tctccccctg atcaaagata ggacgtatat
    27241 caaagcctgc aggaagtgtt ggctttatcg attccaattt tttgtaaaca tttcgaacag
    27301 tcagaacagt atttttgccc gactgtttct ggataagcat taccgtacca ttttcaccat
    27361 tgatgtgtac aatatccttt acctcgccag gctcaaatga aacacgagcg atatcgcgta
    27421 aatagattgg cgtattgttg cgcactgcaa cgacgatgtt gcgcatatca tccagggact
    27481 taaactcacc ctcggtacgg aatgtatatt ttgtgtacgc ggttttgatg tcaccaccgg
    27541 ctatattttg attttcaagg gcaagtgtct gtataatctg gtcaatcgat aatctatagg
    27601 catccattct gctcttaatg agctcaacct tagcttcaag gtcctcacct cctacaacct
    27661 gtgccgaagc aacaccatca gtttgctcta taagattctt tatattatcc tcagcatatt
    27721 tcttgatata tgaccgctcg cgctctccgg ttattgccag tcccataata ggaagcattg
    27781 atatatcaaa ttttacagtt accgggtttt caataccctc aggcagtcta cttcgtacca
    27841 tgtcaatgcg ctcgcggata tcggctactg cctctaccat gtcagtaccc cactggaact
    27901 taatggtgat tgaggacatg ccttctttgg aaacggaagt aacataatca acattattaa
    27961 cggaccctac tgcctcttca agaggtttgg tcaccgagtt ttctatctcc tttggtccta
    28021 cattttcata ttcaacgtat actacagcaa tagggaattc aacatcaggg aaaaagtcta
    28081 attcaataaa gtaataactc acaactgaga gtataataat accaatatac agtatgctaa
    28141 ctgttactgg cctgctaaca gaaatatctg taattttcat accatgaaac tccttataat
    28201 gatgctatcg cttaataatt aatctcttcc atgtatacca gcagtttaac gccgtgcttt
    28261 acattttctt ttcctaatgt taccacaaca tctccatcat tgatacctga tacaacctct
    28321 gttatgtttt tgtgtacata gcctggtgtt attgattgtt taaatgcctt gttgtcgatt
    28381 actttaaaca catagtaatt gccagaagca tcgctcataa tgcagtcgga cgggataatg
    28441 acagcctttt taacaaagcc aagtattatc tgaatatctg caaacatgcc atgcctcagt
    28501 tttaactgag gattatcaat aagtatacgt acctcctgtg ttctgcttgc tgcatcaagc
    28561 aatgcagttt ttcggtacac cgttcccgta aaagaggtgt tggggaatga tactacgcgc
    28621 accttagcct tcataccaat ttgaaccatg ttaatctgct cctcaataag ctgtgccaca
    28681 gcctcaacct gattcatggt taccacctgc gcaagtgggg tttgtgtagt aaccgacatg
    28741 cctctgtcca ccatgatggt gccaacatag cctgttaatg gtgactctac cacagccggc
    28801 atatattcca ttccaattat gtctctgtct atcttgaata caacctgccc ctttttaaca
    28861 tattgccctt cttttacctg aatctccata accttgcctg gtacctgtgg aaatacatga
    28921 acaacctctt tgccaacaat atcaccgctt agatcaaggg tctttgtgat atccatacga
    28981 acagcttttg cagccttaac agcgacaaca tgctcctctt ccacctgttt ttggggattt
    29041 attaactcaa ctacaaacat gaccaatcta taggatgcaa tgagtgcaat tataatagca
    29101 acaatagtta agattgtttt tgctctgcct tttttcatta taaaacctcc atggttcgtt
    29161 tagcaattat tgaattgtaa aataatcaat accagttgcc ttattcaatt cggcctgtga
    29221 taccttataa tcatataatg cctgaatata caatgtttta gcctggataa gagctacatt
    29281 tgcttctaaa aattttgaat tatcaataat accatttcta aactgtatgg tagcagtctt
    29341 taaggattct tcggcagttt ccacattacc ctgctgtgaa tagatagagt ttgacgctga
    29401 ttccaatttt aaaaatccac tctggatatc aaggcgtatg cttttaataa aatcctgtag
    29461 ttgtaaatct atctgatttg tttggctttc taactgttta gctttagcat gggtgctatc
    29521 cagtggtgac catccacccc acttataggt agcaccaaag gttatcatcc agttgtcgtt
    29581 ccagccactg ggagcaaaac t
//
LOCUS       DAOSOX010000011        29514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1583_length_29514_cov_9.136902, whole genome shotgun sequence.
ACCESSION   DAOSOX010000011 DAOSOX010000000
VERSION     DAOSOX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29514
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_29514_cov_9.136902"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1604)
                     /gene="atpA"
                     /locus_tag="PLX22_01645"
     CDS             complement(75..1604)
                     /gene="atpA"
                     /locus_tag="PLX22_01645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOT18637.1"
                     /translation="MKIKTEEIKSIIKKEIEGYKSQLDVSEVGTVIQVGDGIARIYGL
                     DNAMAGEMLEFENGAYGLVFNLEENSIGAVILGDYLSIEEGDTVKRLNRVLAVPVGPE
                     LLGRVVNPLGIPLDNKGPINTTKMRPVEFLAPGIADRQPVKEPLQTGIKAIDSMTPIG
                     RGQRELIIGDRKTGKTAIAIDTIINQKGKGVYCIYVAIGQKASTVASVVEKLEQYGAM
                     DYTIVVTASASDPAPLQYIAPYAGCAMGEYFMYEKNGHSLCIYDDLSKQANGYRQLSL
                     LLRRPPGREAYPGDIFYCHSRLLERAAKLSDDLGGGSLTALPIIETQEGEVSAYIPTN
                     VISITDGQIYLVPGLFASGVRPAVDVGISVSRVGGNAQIKAMKKYAGSLRLDLAQFRE
                     LEAFAQLGTELDPATQRQLDRGQRLVEVLKQVQYEPMPVAEQVAIIYAGTQGYLDKVP
                     VPEVKRFEKEFLKFLRDAHPELLDGISSTGELKDPDKFGKVAGDFVESYLAGKKADVR
                     A"
     gene            complement(1594..2163)
                     /gene="atpH"
                     /locus_tag="PLX22_01650"
     CDS             complement(1594..2163)
                     /gene="atpH"
                     /locus_tag="PLX22_01650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOT18638.1"
                     /translation="MATSEVAKVYAGALLEIAHEKNAIAKVEEELSFLAELLKNDPDL
                     LRYFNAPGINNDSKKQFVDKVFGGKLSDLMVNFLKVLIDNGRQQNIPDINVAFGELLD
                     IINNRQKIKLISSIKLDADIINRIKKSLEDRFNKNIIIEEEVVPAILGGIIVQVDDLI
                     IDASLKKDLIKLREKLIQSKIRGEVAYED"
     gene            complement(2205..2714)
                     /gene="atpF"
                     /locus_tag="PLX22_01655"
     CDS             complement(2205..2714)
                     /gene="atpF"
                     /locus_tag="PLX22_01655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOT18639.1"
                     /translation="MEVLKEGLLKVEPGLLLWTIITFIVLLLILWKAAWKPIVEALDA
                     RAEKIRGDIESAEKSRLEAERLFAEHKAMMDKAKEEAASIIAEGKADAERLRNSIVEK
                     ANQEAKDLIERARREINLAKDKALAEIQSEVVTISTDIAAKIIAKNLKVEDQKALVEE
                     ALQKIRTVQ"
     gene            complement(2737..3021)
                     /gene="atpE"
                     /locus_tag="PLX22_01660"
     CDS             complement(2737..3021)
                     /gene="atpE"
                     /locus_tag="PLX22_01660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOT18640.1"
                     /translation="MEYLGLSYLAAGFGAGLIVFGAALGIGKLATGALEGMARQPELS
                     GDLRTAMIIAAALIEGFTFFALVVTFMLATKAPIATTPVEKPQAEQTEVK"
     gene            complement(3111..3968)
                     /gene="atpB"
                     /locus_tag="PLX22_01665"
     CDS             complement(3111..3968)
                     /gene="atpB"
                     /locus_tag="PLX22_01665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOT18641.1"
                     /translation="MIQLLQSLPVIIQSSKSGEEESMYDIVMHHLTDHPIHDGFIGKI
                     NNLFFHEKLFGIFDMTITRWVIMLWIAALLCLLIFIPVARKIKNSVYGSRSRWVNLWE
                     VLIAFIHDEVVEPNFDHHYVKKAMPYFLTVFFFILFCNLIGLVPGMSTATGNLAVTGG
                     LALCTLIGMLGVGMVKQGIFGYWVGLVPHGVPAFVLPLMYPIEIIGLLIKPFALTVRL
                     FANMTAGHVVIIIFIYLVMMFQSYWVGIGSVAGSLMIYLLELLVAFIQAYIFAVLSAM
                     FIGTSMHAH"
     gene            complement(4023..4295)
                     /locus_tag="PLX22_01670"
     CDS             complement(4023..4295)
                     /locus_tag="PLX22_01670"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOT18642.1"
                     /translation="MKKDVTRIILGYSVIGIQLAITILVFLYGGFFLDQHFNSSPLFV
                     VIGTVLGMGIGFYNLMKSLSELDRLLKRSQQADDSNSSGSKRRKWL"
     gene            4542..4793
                     /locus_tag="PLX22_01675"
     CDS             4542..4793
                     /locus_tag="PLX22_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18643.1"
                     /translation="MKLSIKLIENNNEFIATCPELDISCYATSKHEAIKRIQNILFFY
                     MQSAQELGFSVENFETIDVDGKHYLLRYDICPTTNSTLH"
     gene            complement(5129..>6171)
                     /locus_tag="PLX22_01680"
     CDS             complement(5129..>6171)
                     /locus_tag="PLX22_01680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT18644.1"
                     /translation="FNEPAFHIKQIFLRVRDFKIVNNKRVNLTKSELEKAYGDVENKA
                     REIIGRLKNGEKFEELAKQFSDDFSKKNGGDIGYIVKDMIQSSLYQAINESNIGLLET
                     PIRAENGIYIIQIVEKQNITKKNLNKIIKDEMQAKRLEQILLRKASNNYIDNLMSAQD
                     VQFNEQKCKSKNPKDIIFKVGDTIFTVGDLEKKISYFKHDQPVFHKSVMEDTKKVEIA
                     KNAFKVALLRRAAIKENMHNDEEFKKEVEKRKQMLFAKEYMDYIADRDVIVTPKEVRD
                     EYEKYKDTRFTTYTNQKGKTEKQIISFDKVKDQIAMMIYRKKRADNLAKWKRDMLHIY
                     KFSVNEAELEGK"
     assembly_gap    6172..6271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6272..6669)
                     /locus_tag="PLX22_01685"
     CDS             complement(<6272..6669)
                     /locus_tag="PLX22_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18645.1"
                     /translation="MKKLIAASFVIIIIFAYIGCKCNSDILARYGDTTITRGQFKQWL
                     KDKQYPIETILKNKKQQRDKLELMAIESIALKEMQKDNLQASEKLKFLEDMAFESVLL
                     KALYDKKIKKNTDFNEPAFHIKQIFLRVRD"
     gene            6930..7463
                     /locus_tag="PLX22_01690"
     CDS             6930..7463
                     /locus_tag="PLX22_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18646.1"
                     /translation="MRDKAFYKNRLFSLIHHDAPIDCKTLIEYGYDAIEKGNFIKAFR
                     LFMLGYRLIGNNAEILNGMALSLCELGRLKFSKSILLFALKKFPDNVLLLTNLATVYF
                     DEYNYDKAIFYYTKAISIDPFFIEAHINLVNVYCERGDIFMAYIACLNLLKKHPDNFE
                     AQELCDDIIINMGLSLV"
     gene            complement(7460..7870)
                     /locus_tag="PLX22_01695"
     CDS             complement(7460..7870)
                     /locus_tag="PLX22_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18647.1"
                     /translation="MKNLSYTIKKYLLFLLIIMLSCSKAPVDDAANVVINYLATTDEK
                     KLHGYLDRDSIQYIETLKKDAGTMYPYVWVHHDNNAIWDVVAVSRTANEAWVTVQCVK
                     HKKLNAIGMTIKHKLIKENGEWKISLKGELPKND"
     gene            complement(8001..8246)
                     /locus_tag="PLX22_01700"
     CDS             complement(8001..8246)
                     /locus_tag="PLX22_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18648.1"
                     /translation="MKEFCCYNCLFDVKPIIILNEVPVLSKVKAMDCITELPYHHEHS
                     EKSHRFDNVFHSERSEESHHFMKEEILWDYVPQNDKK"
     gene            complement(8268..9467)
                     /locus_tag="PLX22_01705"
     CDS             complement(8268..9467)
                     /locus_tag="PLX22_01705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT18649.1"
                     /translation="MKLRPAYKRVGMLFSGGPAPSANTVISSVALNFLDNGIPVIGFY
                     RGYEFLQDFNINYPKIKKGVHFEEIDYSITHVRNERGIYLKTSRANPGKDIKTIQDIK
                     DSEKVKKLNNILDAFEYLKIGALISIGGDDTLKTANYLNLLGFPVIHIPKTIDNDYYG
                     IPWTFGYWTAVDTAQKSILNLKADAQSTDSFFIVELMGRKAGWITYAAGIAAEAIMMI
                     AAEDIEEDMLDLEVLVDKITNVILARENNRRPYGVIAVSEGIVDKLSDEYKPKHTDKH
                     GNVLYREAKIGEILAERVKKRYQEKTGMEVKIIAKQIGYETRSAPPISFDVVLGAMLG
                     YGAYKFYNETMFGIMVSVTDNFDLKAVPFSELIDQDTLKTRLRDVPKGSDFYTLKERL
                     SYRKSWE"
     gene            complement(9553..10227)
                     /locus_tag="PLX22_01710"
     CDS             complement(9553..10227)
                     /locus_tag="PLX22_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT18650.1"
                     /translation="MREILNQAIAKRQKALSEFDSKQLMKMAGIPVSRNYLAKSKEEA
                     LDYAQKLGFPVVLKGCSDTLTHKTELGMVKLKLKTKEDVANAYDEITGKGIPLDGVLV
                     QEWLDGDNREFVLGSIKDPQFGPCVMFGLGGVFTEALQDVSFRVAPITSIDAYEMMDE
                     LRTKKLLGPFRGSPAVDKEMLARALVGLSELINTYEEIAEIDINPIIINGDKPVAVDA
                     LVILKQ"
     gene            complement(10220..11686)
                     /locus_tag="PLX22_01715"
     CDS             complement(10220..11686)
                     /locus_tag="PLX22_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT18651.1"
                     /translation="MIESLFNPGSVAVVGASEVPLKWGTYIASNILDGKFNGRFYPVN
                     PNVPEVFGYKTYPSLLDIPEPVDLVFITTPSKTIMHILQDCIEKGITNIVMITSGFSE
                     TGKEGAMLENDIVALAQSRGLHIVGPNTMGIVNTHCSLYATGTITRPKPGGISIIAQS
                     GNLGFQILEWARSQNIGISKFVGSGNEAVLKIEDYLEYYKSDSETKVILMYIEGIDDG
                     KSFVEIAKNTSLKKPIIALKAGRTEIGSKAAASHTGALAGSYSVFKGVVQQTGIVLAE
                     SPKELLSLSAAFDSFPLPKGNRIGIVTLGGGWGVITADECVERGLLIPELPQSIVEEL
                     DKRLPPFWSRGNPVDLVGQPDFQIFKDAVELMVANDAFDVVIVLGIIGAKVFALRAAE
                     SILNLGKMEKSIYDDFMKSLNVLEKEFLDRLTELMAIYNKPIVTVSFSKSFEDETIHD
                     CGGRFKCVIYDTPEEAVLCVAKMYNYYNYVTKRSMVYA"
     gene            complement(11776..>12654)
                     /gene="galE"
                     /locus_tag="PLX22_01720"
     CDS             complement(11776..>12654)
                     /gene="galE"
                     /locus_tag="PLX22_01720"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOT18652.1"
                     /translation="GFKDFVSGFDFIEGDIGDFDKVMFVLKKYDIQCVMNFASFIAVG
                     ESVQNPLKYYENNVAKTLTLFKAMLQSNIKQFIFSSSAAVYGNPDVVPIPEESPMKPE
                     SPYGKTKYCIEEVLKDLTASDSFHAMSLRYFNAAGADESGQIGESHIPETHLIPLVIR
                     AVLDPGYTVTIYGTDYDTPDGTCIRDYIHVNDLGDAHVLALKKLIETNQSDVFNLGNG
                     KGFSVKEVIASVRRVTGRDFKVQEGQRRKGDPAILVASSKKVKLQLQWQPHYTHIDSI
                     VATAWKWESQQKRKGY"
     assembly_gap    12655..12754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12755..13080)
                     /locus_tag="PLX22_01725"
     CDS             complement(<12755..13080)
                     /locus_tag="PLX22_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT18653.1"
                     /translation="MYTYLFGPVPSRRLGISLGVDLIPHKTCTLNCIYCECGKTTKLT
                     IERKEWVPINDVIDELKRYLNENPCPEYITFSGSGEPTLHTGIGIILDFLKEQHGTFK
                     TAVLTNG"
     gene            complement(13082..14101)
                     /locus_tag="PLX22_01730"
     CDS             complement(13082..14101)
                     /locus_tag="PLX22_01730"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOT18654.1"
                     /translation="MHQIIDIIKESLFITMFVVVIMLLVEYINVVTQGKITNLKSKKL
                     QQYCIAIVLGIIPGCLGGFAATAMYAHGAISLGAIVATMLATSGDEAFVMFAMFPKTA
                     LLMNIGLAIIAIAAGYIVDIIFKQHSNSEQCCDGLVIHNEVSFSSYTLHNIAALWKNL
                     SLSRASLMSVLVIIFFMIVTGVFHEEEGFVKVAVPIIVVIAMIIVAIVPEHFLQEHLW
                     EHVFKRHLPRIFLWTVSALFVIHVLVNILHIDASIHHAKWMVMIIAGLVGIIPESGPH
                     LLFVMMFAKGLVPLSVLVTSSIVQDGHAMLPLLAQSRKYFVLIKFINLIIGLFIGSVM
                     MALGY"
     gene            14504..14905
                     /locus_tag="PLX22_01735"
     CDS             14504..14905
                     /locus_tag="PLX22_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18655.1"
                     /translation="MKLQSLKHFVACLFLLPVLMLSCSDEGITIKFMVVSTVKGFDGY
                     YIVDGDSVGYFSATENAYGIAMYEKEIDDVEYLEVSATTRRDATSIEIKVYRDDKKVK
                     SSQTNIEDPFEPTILNLEYSIGEEDEEKTSS"
     gene            14930..15403
                     /locus_tag="PLX22_01740"
     CDS             14930..15403
                     /locus_tag="PLX22_01740"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOT18656.1"
                     /translation="MLNFEIHDATRELSIARALKKYEPPFKVIDSYRLIDDGSNPEAT
                     VQIEANGRYIHEASTGDGPVDALATVLKKALIPLFPEIENVKLIDYRANILDAKRGTS
                     SNVEVTIIFTDGEHVWKVYALSENINAASFRVLLDGFEFAILKKNKKTINKSQEL"
     gene            complement(15381..16535)
                     /locus_tag="PLX22_01745"
     CDS             complement(15381..16535)
                     /locus_tag="PLX22_01745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18657.1"
                     /translation="MKKTLSAIDQAVLIRAIEQSPASIVITDTRGIIQYVNPKFESIT
                     GYSFTEAIGQNPRILKSGHQPKEFYQNMWETILRGEVWDGIFHNKQKNGSYFWEHCII
                     APVADEHGNVTHFVAIKEDITEKKEQEEALRKSEEELRKRNDIINKDLEYAKRVQQSI
                     INTPIPEYPGIKIVTCYRPYDFVGGDFYTIKKINHDLAVFMADITGHGVPAALFSSVL
                     KYATENLWNNGIIQPDEFIHQLNEKLFPILGGYYFTALYGLITPLLDGGFKLTYTRSG
                     HPYPIVLSGDAISMLDSSGIPLGIEKNLKLLVKNIELKKGDRLFLYTDGLIEIDINNQ
                     KDLRFDILTKEIIATKQIALHDAVPKIIEVVTSQNKTLDDDIAVIALEIC"
     gene            16523..17383
                     /locus_tag="PLX22_01750"
     CDS             16523..17383
                     /locus_tag="PLX22_01750"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18658.1"
                     /translation="MFSSCIVTFCSLITRHCTLIHYTIKNNLYEKNLLTVTIKQKKYF
                     NEFLITIVTLLMYNITHSLQYINNIFQCSERKYLDPFSIITFDSAMRKNNRQHILDTA
                     RKLFLEYGYNGISIRNIAKRAKLTTGAIYFHFKNKKEIYSTICLEAINTLHRLFSDGI
                     KLRKTPAQKLISTYDSYLKFYYEFHDYYNVLTEYRAEYDDEPDPYKDEIQKQNLSIQN
                     LMADVFKEGIDQGVFKDLDPKMVAFFLSAVAEGMLQFKKLGVMDAAGITDNKFREFMK
                     IIVGQGIQKK"
     gene            complement(17529..18896)
                     /locus_tag="PLX22_01755"
     CDS             complement(17529..18896)
                     /locus_tag="PLX22_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18659.1"
                     /translation="MNTPFTFEAMIAFGWMSVMLLLGIILRAKVKAFQRFLIPSCLIG
                     GAIGLILRSYEIIPLSADILETLAFHLFNISFISIGLTYNKEKADDKGVGEVLHGSLW
                     MANIEGVSFSIQAIIGGLCVMVLNYLGYNLFNSFGFLTPIGFEEGPGQALSVGKVWEN
                     LGYAHSATLGLTFAVAGYMFSFFIGVPLAYLGIRKGKAVYTPKELSMELRRGVMAQQS
                     EKEVAGELTTHSSNVDALAFQASLVGVVYMITYVVIYGISSLLSPEMGQMMWGFFFFI
                     GLLIAFIVRFIITKLGGLYLIDPGIQHRITGFSVDYLLVATVMAIQVVVVWQYIVPIV
                     VICVLAGIITTIVCYYYGRRLWSVNLERMLLIYGTVTGTVSTGLLLLRIADPEFKTTT
                     AIEAGIMSIFGAPYVLLGTLLVSAPILWGWSIVKTILVFAGMLIVAAAIIELQRYLLL
                     KKMKQ"
     gene            19201..19959
                     /locus_tag="PLX22_01760"
     CDS             19201..19959
                     /locus_tag="PLX22_01760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT18660.1"
                     /translation="MKIIDLSSIIEPSPSGTPDFLKIDIKYHSHGDGAKQVEALLKVP
                     AHLMRNGEGWATETITQLGTHDSTHVDAPWHYNSTIQGKPAPTIDELPLEWFYGNGVK
                     LDMTHKAEGDPVTPQDIEKELKRINYTIQPNDIVLMYTGMDKFYGQPDYIFRGCGVTY
                     EATVMLYNAGVRVMGIDAWGWDMPLNLQAQKALQENKPGIFWSAHQADLIYSHMERLV
                     NLGLLPAKGFKVACFPLNIKRASAAPARVVAIVE"
     gene            20156..20944
                     /locus_tag="PLX22_01765"
     CDS             20156..20944
                     /locus_tag="PLX22_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT18661.1"
                     /translation="MGDTTQNKCHYIIENGIAIITIDNPPMNALNHIVLKDLRNSVNA
                     LIDDAAVRVIIITGAGKAFVAGADINEIKVQNTKEDGANFLKNGQEIYNIIENADKPF
                     IAAINGYCLGGGLELALACHIRLADESAQVGLPEIKLGIIPGYGGTQRSPRLFGKAAA
                     YELILSGNFISGKQAETYRIVNRATQKGTVLKEAQELAKAIAARSRLAVKAAMQAIAQ
                     GYTMEFYTALTFERELFGKLCETEDKKEGIAAFLEKREPQFKDK"
     gene            20949..21395
                     /gene="rimI"
                     /locus_tag="PLX22_01770"
     CDS             20949..21395
                     /gene="rimI"
                     /locus_tag="PLX22_01770"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT18662.1"
                     /translation="MDNLIIRHAHYDELEAIARLDALVFGQNAWPFNSFVNELQLHFS
                     KLLIAELDTTFAGFALAWYIADEIQLMRIAVNPVFQRNKIGSTLIQHITHYAQNQSKI
                     VLEVADNNTQAIAFYTSLGFYRVGVRKNYYHLNSAILMEKIIDHED"
     gene            21385..21963
                     /gene="yihA"
                     /locus_tag="PLX22_01775"
     CDS             21385..21963
                     /gene="yihA"
                     /locus_tag="PLX22_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT18663.1"
                     /translation="MKINTVQFITSATTPNHYPKYSFPEFAFIGRSNVGKSSLLNTIL
                     GRKNIAKTGSKPGVTQTINFYCINDAISLADCPGFGYAKISQSKRREFITMIDNYINI
                     RSSLKLVFLLIDIRREPDEHEINIINKSLHKNINVMIVATKCDKVSKNEINAGIKRIC
                     TSLNLHPESVIPSSKTGMGKNIILSVISKHCQ"
     gene            complement(21972..22571)
                     /locus_tag="PLX22_01780"
     CDS             complement(21972..22571)
                     /locus_tag="PLX22_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18664.1"
                     /translation="MKLVSDSTKVVYLILMIVFIVIVGLFWMDYIGLINLGKIYHHYI
                     VTEAPSVVDAADDEPSLIEREEFEKEKDKLLQRIEELDKREAKITEAEKVISKEKEKL
                     EQLQKGLDQEKKALELEKKKYSGYKKNVMDLAKKIESIPPQDAVDIMVQWEETLIIDV
                     LRQIDQNAAKEGRMSISTYLLSLMPKEKASRVMYLMTQL"
     gene            complement(22604..22954)
                     /locus_tag="PLX22_01785"
     CDS             complement(22604..22954)
                     /locus_tag="PLX22_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18665.1"
                     /translation="MDINSIQQMQKRIAEIKSRFAAIQKTYNRISPSEKTTVQNTPNA
                     MQQNTNQQPDIKGTIEQKNNTFDNKITEEILKSVLYNKADSKASSDMQQIQNLLKKAI
                     SQYTSKGDLFNIEE"
     gene            complement(22962..23405)
                     /gene="fliJ"
                     /locus_tag="PLX22_01790"
     CDS             complement(22962..23405)
                     /gene="fliJ"
                     /locus_tag="PLX22_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOT18666.1"
                     /translation="MKKFQFRLQTLLDIRISKEHAIKAELAMLIAKQNEEIVMQEEYR
                     KKIKEYYNKLSKKMRDGTFSYNEALMCERFAYNAQKAIDLSKKRVEQMQPEIDRVRQK
                     LMQASKERKVVERLKEKKYEEYMYQFNREMGKELDDLNQRIYNAM"
     gene            complement(23429..24835)
                     /locus_tag="PLX22_01795"
     CDS             complement(23429..24835)
                     /locus_tag="PLX22_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOT18667.1"
                     /translation="MIQILPHEKVDIFTKYKEVIEEVETIRATGRVEKITGLTIESHG
                     PDVQYGELCRIRLKDGGYLNAEVVGFNKNKIILMPIGDMKGVVPGADVYAVGSQLIVP
                     VGDELVGRVIDGMGNPIDGKGNIYLKEGYPVDNKPLRPLEREIIDSPLSTGIRAIDGL
                     ITVGKGQRIGIFSGSGVGKSTLISMIARNTAADISVIALIGERGREAKDFVEKELQNE
                     GLKRSVVIISTSDQPPMLRLRAGFLAHSVAEYFRDKGLHVNLLMDSITRFAMAQREVG
                     LAAGQPAATRGYPPSVFSLLPRLLERAGNKKCAGSITGFYSILVEADDMNEPIADAVR
                     GILDGHIVLDRKLAHKNHYPAIDVLGSISRCMNDVIDEEHKKAANKFRTLLAAYHEAE
                     DLIMLGAYARGSDPDVDAAIDMKPSMDEFLKQGIYEKDIYENIKTKLIELMFGSKVKQ
                     EQKRDFVSREPYFVQRNR"
     gene            complement(24869..25795)
                     /gene="fliH"
                     /locus_tag="PLX22_01800"
     CDS             complement(24869..25795)
                     /gene="fliH"
                     /locus_tag="PLX22_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOT18668.1"
                     /translation="MSKLVFKPAEIQITATPKQIELPDKYQKNILTDEEYAEFEVDEQ
                     GNPVEVYQGPSIEEMEAELERYRRETEEEVRRMLDEAKARAAQIEEDGKKAAFLELQQ
                     AREQIKIEMERLKIESEREVERGKFEAEKMIKEAELKVSEIEHEAYKRGYDAGREEGY
                     KEGQAEVMRLIDRLGTIVSTAVDIRDDIIRSSEKLMTEMILMIARKVIKDEIVERREV
                     VINNIKEALQRVKDRDRIDIRVNFADLDMTTAHKDELIKMMESLKKVNIYEDSRVERG
                     GCIIETDVGAIDARISTQLEAIEEAIRNARPL"
     gene            complement(25788..26804)
                     /gene="fliG"
                     /locus_tag="PLX22_01805"
     CDS             complement(25788..26804)
                     /gene="fliG"
                     /locus_tag="PLX22_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOT18669.1"
                     /translation="MLQQKKSQLTGRQKAAIFLVSLGSDVSSEIFKHLREDEIEQLTF
                     EIARLDKVEPEDRDKVLMEFQELMMAQEFIATGGIDYAREVLERALGTQKAIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQGEHPQTIALILAYLDPQKAATILSGLNHQIQAD
                     VARRIATMDRTSPDVLREVERVLERKLSTLASEDYTSAGGIDSIVEVLTHVDRGTEKI
                     IIEALEEEDPELAEEIKKRMFVFEDIVLLDDRAIQKVLREVDTQDLAKALKGVDAEVQ
                     EKIFRNMSKRASALLREDMDFMGPIRLRDVEEAQQKIVNIIRKLEEAGDIVVARAGEE
                     ELVV"
     gene            complement(26943..28640)
                     /gene="fliF"
                     /locus_tag="PLX22_01810"
     CDS             complement(26943..28640)
                     /gene="fliF"
                     /locus_tag="PLX22_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOT18670.1"
                     /translation="MDLFTKIFQQLKEIYAKLDTTKKIIIGVVTGVLVIAFIVLFSVS
                     SQKANIVLFSDLPSDEFGQVTKKLEEMGFHYTTSGTTTILVDPNQREVILTRLAQENM
                     IPRGIPGWKLFDISKWTETDKELNVKYMRALRDEIKRHIESLKNIEKADVEIAITEDE
                     LFTEKENAYTAAVTVYLAPGYDTLSKKEIKGIQYLVSRAVGTKLKPENVSITDEYGKI
                     ISDFDDQFDKAKEEFTLLEYRKKIEEKARVQMLKDIRLGLEKIFSSDRIQIVRLNMDF
                     NWDKIQEEQEEYTPIEMEKDNPLTPYSERKVKDALTISKKSTKEHFQGHGWNPEGPAG
                     TEGNKPPGYKASDDQFSQYDKEENVENNAINKTIRKIERDPFDISAVSVAIAIDGYQD
                     LPRLPDGSYDLDPAKKPVQLPLSEDELRQAENIVKKAINFNAARGDQVAVENIMFDRT
                     KQWDIAREEFRKKEQFKRMLLAALVGIMALFLGVILFRAISRELARRRRIREEQLALE
                     QQRMREAALRAAEEEGIDVELSLEEKARLELQQNAVNLARERPDDVAQLLRTWLAEE"
     gene            complement(28867..29196)
                     /gene="fliE"
                     /locus_tag="PLX22_01815"
     CDS             complement(28867..29196)
                     /gene="fliE"
                     /locus_tag="PLX22_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOT18671.1"
                     /translation="MVQESNFISKSNIFTMKTTHPLHYDNKPEKIQDDDVAGSFMDAL
                     KSTLGKVNDLQVDAEDLTQQMIYEPESVDIHTVMIAQQKAEIALSLTKAVRDEAIRAY
                     RELINLR"
     gene            complement(29180..29350)
                     /locus_tag="PLX22_01820"
     CDS             complement(29180..29350)
                     /locus_tag="PLX22_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18672.1"
                     /translation="MYFEKGHINEICCSHELSTPITLSRRKKEWLQFGIGERQIVESR
                     IISGSVNYGTGK"
     gene            complement(29374..>29514)
                     /locus_tag="PLX22_01825"
     CDS             complement(29374..>29514)
                     /locus_tag="PLX22_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOT18673.1"
                     /translation="EMPNVNVVMEMTDLISASRSYEANVMMINNAKSMFSKALEIGKG
                     GV"
BASE COUNT         9135 a         6037 c         4318 g         9824 t
ORIGIN      
        1 tgtattctta acagaattaa ttcgctttaa tatttctcgt tgtgttgcca cagctattcc
       61 tcttttttag tttattacgc tcttacatct gctttttttc cggctagata gctttcgaca
      121 aaatcacccg ctactttccc aaatttatcc ggatctttaa gttcgccagt tgatgaaatt
      181 ccatctaata attcaggatg cgcatcgcgc aaaaatttta aaaattcttt ctcaaatctt
      241 ttcacttcgg gtactggaac tttatcaaga tacccctgag ttcctgcata tataatagca
      301 acctgctcag caacaggcat tggctcatat tgaacctgtt ttaatacctc aacaagtctt
      361 tgacctctat cgagctgacg ctgtgttgca ggatcaagct cagttcccaa ttgagcaaaa
      421 gcttcaagtt cacgaaactg tgctaaatca agtcgtaatg aaccagcata ctttttcata
      481 gctttaatct gagcattccc gcctactcgt gatacagata taccaacatc aaccgcaggc
      541 cgcacgccag atgcaaataa accaggtaca agataaattt gcccatcggt aattgaaata
      601 acgttggtag gaatatatgc tgaaacctca ccttcctgtg tttctataat tggtaatgca
      661 gtcaaagaac caccaccaag gtcatccgaa agttttgcag cacgttcaag aagtcgtgaa
      721 tggcagtaaa aaatgtcacc tggatacgct tctcgtcctg gaggccttct aagcaacaaa
      781 gaaagctgac gataaccatt cgcctgtttt gaaaggtcat catatataca aagggaatgg
      841 ccattcttct catacataaa atattcaccc attgcacaac ccgcataagg tgcaatatat
      901 tgtagtggtg ctgggtcaga agcactggca gtaacaacga tagtataatc catcgcacca
      961 tactgctcta atttttcaac aacagatgca acagtagaag ctttctgccc gatagccaca
     1021 taaatgcaat atactccttt acctttctga ttgataattg tatcaatcgc tattgcagtt
     1081 ttcccggtct tacggtcacc aattatcaat tctcgctgtc cgcgacctat cggtgtcatt
     1141 gagtcgatag cttttatacc agtttgtaat ggttctttta ctggctgtct atcggcaata
     1201 ccaggagcta aaaattcaac aggccgcatc tttgttgtat taattggtcc tttattatcc
     1261 aatggtatac ccagaggatt aactactcta cctaacagct ctggaccaac aggtactgcc
     1321 aaaaccctgt tcaatctctt aaccgtatcg ccttcttcaa ttgaaagata gtcacctaaa
     1381 ataacagcac cgattgaatt ctcttccagg ttaaaaacca gaccatatgc accattttca
     1441 aattcaagca tttcaccagc cattgcatta tctaaaccat aaattcgtgc aattccgtca
     1501 ccaacctgga taacagtacc aacttcacta acatccaact gtgacttata cccttcaatc
     1561 tccttcttaa taatcgattt tatttcttca gttttaatct tcataagcca cttcacctct
     1621 gatcttactt tgtattaatt tttcacgtaa ctttattaga tcttttttta aggatgcatc
     1681 gataattaaa tcatctactt gcacaataat cccgcctaaa attgctggta caacttcttc
     1741 ttctattatt atattcttgt taaaacgatc ttctaaactc ttttttattc tatttatgat
     1801 atcagcatca agctttatac tgctaattag tttaattttc tgtcgattat tgattatatc
     1861 aagtaattca ccaaaggcga cattaatatc tggtatattt tgttgacgtc cattatcaat
     1921 tagtaccttt aaaaagttaa ccatgagatc agataattta ccaccaaaca ccttatcaac
     1981 gaattgtttt tttgaatcat tattaattcc aggcgcatta aaataacgca aaagatcagg
     2041 atcatttttt aataattctg caagaaaact taactcttct tcaacttttg ctatagcgtt
     2101 tttctcatgc gcaatctcta ataaagcacc tgcataaact ttcgctactt cacttgttgc
     2161 cacaccgata accctattaa ttaaaattta aggacagttc agtactactg aactgtcctt
     2221 attttttgta atgcctcttc tacaagggct ttctgatctt caactttgag attcttagct
     2281 ataatcttcg ctgcaatatc cgttgatatt gtaacaacct cagattgaat ctctgccagc
     2341 gctttatctt ttgcaagatt tatctcccga cgcgctcttt caataagatc tttggcttcc
     2401 tgatttgctt tttcaactat ggaatttctt aacctttccg catcagcctt accttcagcg
     2461 atgatacttg cagcctcctc ttttgcctta tccatcattg ctttatgctc agcaaatagc
     2521 ctttcggcct caagacggga cttttccgct gattcgatat cacctcgaat cttctcagcc
     2581 ctggcatcaa gcgcctctac aattggcttc catgcggctt tccataatat caataacaac
     2641 actataaagg ttattattgt ccataacagg aggccgggtt caacctttag caagccttct
     2701 tttaaaactt ccatagcgaa ctacctacaa taaaatttac ttaacttcag tttgctctgc
     2761 ttgaggtttc tcaaccggcg ttgtagcaat tggagctttt gttgcaagca taaaagttac
     2821 aacgagagca aaaaaagtga aaccttcgat aagagcagca gcaataatca ttgctgttct
     2881 taagtcacca cttaattctg gctgacgagc cataccctct aaagcaccag tagctaattt
     2941 accaataccg agtgccgcac caaaaactat caaaccagct ccgaaacctg ctgcgaggta
     3001 agaaaggcct aaatattcca ttgactcctc ctcgaaaatt ttgaatatta aattctaaca
     3061 attatgttat tgctaagaaa tatattgatt acctcttaat aatgcattgc ttaatgtgca
     3121 tgcattgagg taccaataaa cattgccgaa agtactgcaa atatatatgc ctgtataaaa
     3181 gcaaccaaca actcaagcaa atatatcatc aatgaacctg ctactgaacc tatgccaacc
     3241 cagtaactct gaaacatcat taccagataa ataaatatta ttattacaac atgtcctgct
     3301 gtcatatttg caaaaagtcg gactgttaaa gcaaatggct taattaataa tccaataatt
     3361 tcaatgggat acattagcgg caaaacaaac gcaggcaccc catgtggtac aagccctacc
     3421 caatacccaa aaataccctg cttgaccatt ccaactccca gcattccaat tagtgtacaa
     3481 agcgccaatc cacccgtaac agccaggttg ccagttgcag tagacatacc tggaacaagt
     3541 cctatcaaat tacaaaaaag aataaagaaa aaaacagtaa ggaaataagg cattgccttt
     3601 ttaacataat gatgatcaaa atttggctcc acaacctcat catgtataaa tgcaattaac
     3661 acttcccata gattaaccca gcgggacctg gaaccataaa cagaattttt tattttacgg
     3721 gctacaggaa taaaaataag taagcagagc aaagcagcta tccacagcat tataacccat
     3781 ctggtgatag tcatatcgaa aataccaaaa agtttttcat gaaaaaaaag attattgatt
     3841 ttaccaatga aaccatcatg aatagggtga tcagtaagat ggtgcatgac tatatcatac
     3901 atcgactctt cttcaccgct ttttgaagat tgaataataa ctggtaaact ttgtagcagc
     3961 tgaatcataa ctttacatac ctcatcaact ttttttgtta ttgtattgat gcaacgtcat
     4021 tgttacaacc atttacgcct tttagaaccg ctactgttac tatcatctgc ctgttgggat
     4081 cgtttcagca atcgatccaa ctcggataaa cttttcatca aattgtaaaa acctattccc
     4141 attccaagta cagtacctat aaccacaaac agaggactgc tattgaaatg ttgatcaaga
     4201 aaaaaaccac cataaagaaa aacaagaatc gtaattgcaa gctggattcc tataactgaa
     4261 tatccaagta tgattcttgt aacatccttt ttcacttaca cctcaaatat cagcgttgga
     4321 ttccatacaa acaccatagc tttttttgtc aacaaaataa gtcttatttt ttttaaaaaa
     4381 tattattgtt atcatatgca attttatatg tcaaatataa ttatcctatt tattttgatg
     4441 taacaataaa aattagaatt actttttgat attatctttt tcttttatat agtcgataca
     4501 ataaataatt agaatgtaat cgcttatgct gaggtttaca tatgaaatta tctattaagc
     4561 taatcgaaaa taataatgaa tttatcgcaa cctgccctga acttgatatt agttgttatg
     4621 caacaagcaa acatgaagca ataaaaagaa tacagaatat attatttttt tatatgcaat
     4681 cagctcaaga attaggattt agcgtagaaa attttgaaac aattgatgtt gatggcaaac
     4741 attatctcct tagatatgat atctgtccga caaccaattc aactctccat taagtaaaat
     4801 caagaaattg ttttatacaa ttaatataca taatatacac tgtcccgatt accgataata
     4861 cgatgcaaag gaataataaa tggcgaggag tttacccatg accggcctgc agccacctat
     4921 gctggatgga aatattttgt agcaatcctc ctgaactttg attcaggatc taattattga
     4981 aattccaggt taagctcaga atgatgttta taaataattc tattttcagg gaaaaaataa
     5041 tttttttgac aacatgattc ctccttgcca tgcttatctt aacacatcct atccatgtta
     5101 atcattgaaa ttttttaaca atatattttt attttccctc aagctcagcc tcattgacac
     5161 taaatttata gatatgcaac atatcccgtt tccattttgc taaattatca gctctttttt
     5221 ttctataaat catcattgct atttgatctt ttactttgtc aaaagatata atctgctttt
     5281 cagttttacc tttctgattt gtataggttg taaagcgtgt atctttgtat ttttcatatt
     5341 catcacgtac ttctttaggg gttacgatta catccctatc ggcaatataa tccatatatt
     5401 ctttagcgaa taacatttgc tttcgttttt ctacttcctt tttgaattct tcatcattat
     5461 gcatattttc ctttatagct gctcttctca gcaatgctac tttaaatgca ttctttgcta
     5521 tttcaacttt ttttgtatct tccatcacag atttatgaaa cactggttga tcatgtttaa
     5581 aatatgaaat ctttttttcc agatccccaa cggtaaaaat agtgtcccca actttaaaaa
     5641 taatatcttt gggattttta cttttacatt tttgttcgtt aaattggaca tcctgtgcag
     5701 acattaaatt atcaatataa ttgtttgatg ctttcctgag taaaatctgt tcaaggcgtt
     5761 tagcttgcat ctcatcttta attattttat tcaaattctt ctttgtaatg ttctgtttct
     5821 caacaatttg aattatatat ataccattct ctgcccggat tggcgtttct aacaatccaa
     5881 tattagattc attgatagcc tggtatagcg aagattgtat catgtcttta actatatatc
     5941 caatatcccc gccatttttc tttgaaaagt catcagaaaa ctgctttgcc agttcttcaa
     6001 atttttcacc attttttaat ctgccaatta tttctctggc tttattctca acatcgccat
     6061 aagccttttc cagttcggat tttgttaaat ttacacgctt attgttaaca attttaaagt
     6121 ctcttactcg caaaaatatt tgtttaatgt ggaatgctgg ttcattgaaa tnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naagtctctt actcgcaaaa atatttgttt
     6301 aatgtggaat gctggttcat tgaaatctgt gtttttcttg atttttttat cgtataatgc
     6361 tttcaacaaa accgattcaa aagccatatc ttctaaaaat ttaagctttt cagatgcttg
     6421 caaattatct ttttgcatct ctttcagagc tatagattca attgccataa gctccagttt
     6481 atctctttgc tgttttttat tttttaaaat tgtttcaatt ggatattgtt tatctttcaa
     6541 ccattgttta aactggcccc gtgtaatagt agtgtcacca tagcgtgcaa ggatatcaga
     6601 attacattta cacccaatat aagcaaatat aataattatt acaaacgatg cagcaataag
     6661 ttttttcatg atataatcct ccggaaataa aatggtaaat taagaaaaga atcatgtttg
     6721 ttaaacaata tcatggaata aaatatatct ttattcattc aattttatat tttctgaaaa
     6781 tctatatggc aaaaaccaat attttttgca attaactttt taaaaaaaat tattttaatt
     6841 gctattgaca gaattgaata tttaattatt gtgctcacat tagctctaga ttaagatttt
     6901 acaataattt tataagtgat agttacctta tgcgtgacaa agctttttat aaaaatagat
     6961 tgttttcgtt gatacaccat gacgctccca ttgattgtaa aacattaatt gaatatggat
     7021 atgatgctat tgaaaaagga aacttcataa aagcattcag attgtttatg ttaggttatc
     7081 gtttaatagg gaataatgct gaaatcctta atggtatggc actttcatta tgcgagcttg
     7141 gtagattaaa attttctaaa agtattttgt tatttgcact aaaaaaattt cctgataacg
     7201 tattattatt aacaaatctt gcaactgtat actttgatga atataattat gataaggcaa
     7261 tcttttatta tacaaaagca atttccattg atcctttttt tattgaagcc catataaatc
     7321 ttgttaatgt ctattgtgag cgtggtgata tctttatggc atacatagcc tgccttaacc
     7381 ttttaaaaaa acatcctgat aattttgaag ctcaagaatt atgtgacgat atcattatta
     7441 atatgggttt gtcactggtt taatcgtttt ttggcagttc accttttaaa ctaatcttcc
     7501 attccccatt ttcttttatc aatttgtgtt taattgtcat gcctatagca tttagttttt
     7561 tatgtttaac gcattgtacg gttacccatg cttcgttggc tgttctggac actgcaacaa
     7621 catcccatat agcattgtta tcatgatgca cccatacata aggatacatt gttccggcat
     7681 ctttctttaa tgtttcaata tattgtattg aatctctatc cagatagcca tgcaatttct
     7741 tttcatcagt agttgcaaga tagttaataa caacattagc tgcatcatca acaggagctt
     7801 tgctgcatga tagcattatt atcaaaagaa acagtaaata tttttttatg gtatatgata
     7861 gatttttcaa atagcatcct ccaaaaatag aattattaac atttatcact atgagcttaa
     7921 tacggaatat caatcatcgt caaatatcct gaaatgaatt ctggatggca caccacaaat
     7981 acattattct gaaaaacaca tcactttttg tcattctgag gaacatagtc ccaaagaatc
     8041 tcttctttca taaaatgatg agattcttca cttcgttcag aatgaaagac attatcaaaa
     8101 cgatgcgatt tttcactgtg ttcatgatga tatggcaact ctgtaataca atccattgct
     8161 ttgactttac tcagcacagg tacttcgttt aaaataatga ttggctttac atcaaagaga
     8221 caattatagc agcaaaactc tttcaccagt tactatcgca cataatatta ttcccatgat
     8281 ttgcgatagg ataaacgttc tttcaaggta tagaagtcac tccccttggg aacatcacgc
     8341 aatcgtgttt taagagtatc ctgatcaatc aactcactga atggcactgc cttcagatca
     8401 aaattgtcag ttactgaaac cattattcca aacatggttt cattgtaaaa tttgtacgct
     8461 ccatagccca gcatcgcacc taataccacg tcaaagctaa taggtggtgc cgaacgcgtt
     8521 tcgtagccaa tctgtttggc aataatttta acctccatgc cagttttttc ctgatatcgt
     8581 ttctttacac gctctgcaag tatctcccca attttagctt ccctatacag gacatttcca
     8641 tgtttatcag tatgctttgg tttgtactca tcagaaagct tatcaacaat accttctgaa
     8701 acagctataa ccccataggg acgtctgttg ttttcgcgtg ccagtataac attggtaatt
     8761 ttatcaacta acacttctaa atcaagcata tcctcttcaa tatcttcagc agcaatcatc
     8821 attattgcct cagccgctat tcctgctgca taggtaatcc accctgcttt gcgccccata
     8881 agctcaacaa taaaaaaact atctgttgac tgagcatctg ccttgagatt aagtattgat
     8941 ttttgtgctg tatcgacagc cgtccaataa ccaaaagtcc atggaatacc atagtaatca
     9001 ttatcaattg ttttggggat gtggataaca gggaacccta acaggtttag gtaatttgca
     9061 gtttttaatg tatcatcacc tccaatcgat atcaaagccc caatttttag atattcaaat
     9121 gcatctaata tgttgttaag cttttttact ttttcagaat ctttgatgtc ctgaattgtt
     9181 tttatatctt ttcctggatt tgctcgtgat gtttttagat atattccacg ctcattgcgg
     9241 acatgagtta ttgaatagtc tatctcttca aaatggacac cctttttgat tttaggatag
     9301 ttaatattaa agtcctgtaa aaattcatat cccctgtaaa agccaataac gggtatacca
     9361 ttgtctaaaa agtttaaagc aacagatgat ataacggtat ttgcactggg cgcaggacca
     9421 ccagaaaaaa gcatcccaac ccttttgtaa gcaggccgta atttcatata caatcctcgt
     9481 tttttaaaat tctataataa gcaatcccgg ttaaataata cttttatata tgttttatag
     9541 caattaaaat ttttattgtt ttaagataac cagtgcatca actgccaccg gtttatcacc
     9601 attaatgata attggattta tatcaatctc agctatttct tcatatgtat tgataagttc
     9661 acttaaacca actaaagctc tggcaagcat ttctttatca acagcaggcg aacctctaaa
     9721 cggtcctaat agtttttttg tcctcaattc atccatcatt tcatatgcgt ctatactggt
     9781 tatgggagca accctgaatg aaacatcctg caatgcctcg gtgaatacac cacccaaccc
     9841 aaacataaca catggaccaa actgtggatc tttaatagaa cctaatacaa attccctgtt
     9901 atcaccgtca agccactcct gtaccagaac accatcaagc ggtatacctt taccagtaat
     9961 ttcatcatag gcattggcta catcttcttt tgtcttcagt tttaacttta ccattcctaa
    10021 ttcagtttta tgagttagcg tatcagaaca tcctttcaaa accacaggga aacccaactt
    10081 ctgtgcataa tcaagagctt cctctttgct ttttgccaga tagttccttg aaacaggtat
    10141 ccctgccatt ttcattaatt gttttgaatc aaactctgat aatgcttttt gtcgtttagc
    10201 tattgcctga tttaaaattt cacgcatata ccatactcct tttggtgacg taattataat
    10261 agttatacat ctttgcaaca cacaacacag cctcttcggg tgtatcataa atgacacact
    10321 taaacctgcc accgcaatca tgaattgttt catcttcaaa agattttgaa aatgataccg
    10381 ttactatagg cttgttgtaa attgccatta attctgttaa tctgtcaaga aattcctttt
    10441 ctaatacgtt taatgatttc ataaaatcat cataaatact tttttccatt tttcccagat
    10501 ttaaaattga ctctgcagca cgcaaagcaa aaacttttgc gccaattatt cccaatacta
    10561 tcactacatc aaatgcatca ttagcaacca tcaattcaac agcatcttta aaaatctgaa
    10621 aatcaggttg cccaaccaga tcaacaggat tacctctgct ccagaacgga ggtaaccgtt
    10681 tatcaagctc ttctacaatt gattgtggca actcaggaat taaaagtcca cgttcaacac
    10741 attcatctgc tgttatcact ccccagccac cacccaatgt aacaatacca attctattcc
    10801 cttttggtaa agggaatgag tcaaaagctg ctgaaaggct caaaagctct ttaggtgatt
    10861 cagctaaaac aattcctgtt tgttgtacaa cacctttaaa tacagaatat gaaccagcca
    10921 gtgctcctgt atgtgatgct gctgctttgc ttcctatttc agttctccct gctttcagcg
    10981 ctattattgg cttttttaat gaagtatttt ttgcaatctc aacaaaactc ttgccatcgt
    11041 caattccttc aatatacatc agtattactt ttgtttcact atcgctttta taatattcaa
    11101 gataatcttc aatctttaat actgcctcat tacctgaacc aacaaattta gatattccaa
    11161 tattctgact tcttgcccat tccaaaatct ggaatcccag attgccgctt tgtgcaataa
    11221 ttgaaattcc gccaggtttt ggccgggtga tagttcctgt agcatacaat gagcagtgtg
    11281 tattgacaat acccatagta ttaggtccaa caatatgcaa tccccgtgat tgagctaatg
    11341 caacaatatc attttctaac attgcccctt ctttgccagt ttcactaaaa cctgatgtta
    11401 tcataacaat attggttata cccttctcta tgcaatcctg taatatgtgc ataattgttt
    11461 tagaaggcgt tgttataaat acaagatcta ccggttctgg tatatccaat aatgaaggat
    11521 atgttttata tccaaaaacc tcaggaacat taggattaac aggataaaac cttccattaa
    11581 attttccatc caaaatattg cttgcaatat atgttcccca tttcaatgga acttcagaag
    11641 caccaacaac agcaactgag cctggattaa acaaactttc aatcatagtt tacctcatgg
    11701 acattaatat ataatttgtt tactatcaat gaatgaatat tatatttgta tgtcaacaat
    11761 tttgttagat atatttcaat agccttttct cttttgctgt gattcccatt tccaggctgt
    11821 tgcaacaatt gaatcaatat gggtatagtg aggttgccat tgcaactgta gtttcacttt
    11881 ttttgatgaa gccaccaaaa ttgcagggtc cccttttctg cgttgacctt cctgtacttt
    11941 aaaatcccta ccggtgacac gccttactga tgcaataacc tcttttacac taaaaccttt
    12001 accattgcct aaattaaaaa cgtcactttg atttgtttca ataagctttt tcaatgctaa
    12061 aacatgtgca tcacccagat cattgacatg gatataatcc cgtatgcatg ttccatccgg
    12121 ggtatcatag tcagttccat aaattgttac agtatatcct ggatcaagca cagcacggat
    12181 taccagcggt attaaatgtg tttcggggat atgactttca ccaatttgcc cgctttcatc
    12241 agctcctgct gcattaaaat agcgtaaact cattgcatgg aatgaatctg atgcagttaa
    12301 atcctttaac acctcttcaa tacaatattt tgtttttcca taggggcttt ccggcttcat
    12361 aggactttct tcaggaatag gaacaacatc tggatttcca tatacagcag cagatgaaga
    12421 gaatataaat tgtttgatgt tggattgcaa cattgccttg aacagggtca gcgttttagc
    12481 aacattattt tcatagtatt ttaatggatt ctgcactgat tcgccaactg caataaagga
    12541 cgcaaaattc ataacacact gtatatcata cttttttaat acaaacatca ctttatcaaa
    12601 atcgccaata tcaccttcaa taaaatcaaa acctgaaaca aaatctttaa atccnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccattg gttaatacgg ctgttttaaa
    12781 cgttccatgc tgttctttta aaaaatcaag tatgatgcca atgccagtat gcaatgtggg
    12841 ttcgcccgat cctgaaaagg taatatattc cgggcaggga ttttcgttta agtaacgctt
    12901 caactcatca atgacatcat ttattggtac ccattctttt cgctctattg tcagtttggt
    12961 ggttttaccg cattcacaat agatacaatt aagcgtacat gttttatgcg ggataagatc
    13021 aactcccagc gaaattccta accgccgtga aggtacaggg ccaaaaagat aggtatacat
    13081 attaatatcc caatgccatc ataaccgatc caataaacaa tccaataata agattaataa
    13141 atttaatcaa tacaaaatat ttccttgatt gtgctaataa cggcaacatt gcatggccat
    13201 cctgaacaat ggagcttgta accagcacag atagcggcac caaccctttc gcaaacatca
    13261 taacaaataa aagatgggga cccgactcag gtattatccc tactaatccg gcaataatca
    13321 ttaccatcca ttttgcatga tgaattgaag catcaatatg taaaatattc accaatacat
    13381 ggataacaaa tagcgccgat actgtccata gaaatattct gggaagatga cgtttaaaaa
    13441 catgttccca taaatgctcc tgtaaaaaat gctcgggaac tatcgccact ataatcattg
    13501 caattactac tattatcggt acagcaacct ttacaaaacc ttcttcttca tggaatacac
    13561 cggttactat cataaaaaat attattacta atactgacat caacgaagcc cttgacaatg
    13621 ataggttttt ccacaatgca gcaatgttgt gcagcgtata agaagaaaaa gatacttcat
    13681 tatgtataac aagtccatca cagcattgtt cactattact gtgctgttta aaaataatat
    13741 caacgatata tcctgctgct atagcaatga ttgcaagccc tatattcatc agcaatgcag
    13801 tttttggaaa cattgcaaac ataacaaagg cttcgtcgcc actggtggca agcattgtgg
    13861 caacaatagc acctaatgat attgccccat gcgcatacat ggctgttgcc gcaaaaccac
    13921 ccagacaacc agggataatt cccagtacaa ttgctataca gtattgctgt aatttttttg
    13981 attttaaatt agtaattttt ccttgagtta caacgtttat atactcaacc agaagcatta
    14041 taacgacaac aaacatagta ataaacaatg attcttttat tatatcaatt atctgatgca
    14101 tattgttcct ctattttcct ctatatagta aatagcatag tataaacatc gttacaaatt
    14161 ttgtatatat caaattttaa aaatttaaat acattgctat caagaattat acctctatag
    14221 agtttttata taaaatggat gttcatgttg ccgtggtatt gcgtatacat aaattcattg
    14281 caacaaacag tattgtgcta tgctattgca attttagcaa cattaaaata actagtacaa
    14341 ttttttgctt taaaattatt gacaaaacca ttatataact atttaaattc atgagcaatt
    14401 aaggtaatca tgaattttca atgaattcat tatatttttt atatatacca tatttttgta
    14461 atatttcttt atgctcggta ctaatcagaa taaaggtaac aacgtgaaac ttcagagttt
    14521 aaaacatttt gttgcatgtc tatttctttt accagtactg atgctttcat gctcggatga
    14581 aggtattacc ataaagttca tggttgtatc aaccgttaaa ggatttgatg gttactatat
    14641 tgttgacggt gattctgttg gatattttag tgctactgaa aatgcctatg gcattgcaat
    14701 gtatgagaaa gaaatagatg atgtggagta tcttgaggtc agcgcaacaa ctcgtcggga
    14761 tgcaacatct attgaaatca aggtataccg tgatgataaa aaggttaaaa gcagtcagac
    14821 aaacattgaa gacccttttg aacccacaat acttaatctt gaatattcaa ttggcgaaga
    14881 agatgaagaa aaaacctcca gctaaattaa ttcaaaggca ggatgtgcta tgttaaactt
    14941 tgaaatacat gatgcaacac gtgaactttc aatagcgcgt gcattaaaaa aatacgagcc
    15001 cccctttaag gtaatcgata gctaccggtt aattgatgat ggatctaatc ctgaagcaac
    15061 cgtacagata gaagccaatg gacgatatat ccatgaagct tccaccggtg acggaccggt
    15121 agatgcactg gcaaccgtat tgaaaaaagc actcatacca cttttccccg aaatagaaaa
    15181 tgtaaagctt attgactatc gtgccaatat actggatgcc aagcgcggta ccagcagcaa
    15241 tgttgaagta accattatct ttactgatgg tgaacatgtg tggaaggtat atgcactttc
    15301 agaaaatatc aatgcagctt cattcagggt tttgcttgat ggatttgaat ttgctatctt
    15361 aaaaaaaaat aaaaaaacca tcaacaaatc tcaagagcta taactgcaat atcatcatca
    15421 agggttttgt tttgtgatgt taccacttca attatttttg gcactgcatc atgcagtgca
    15481 atttgttttg ttgcaatgat ttctttagtg agtatgtcaa accgtaaatc tttttgatta
    15541 ttgatatcta tttcaattag accgtcagta tataaaaata atctatcccc ttttttcaat
    15601 tcaatatttt ttactaaaag tttgagattt ttctctatac ccagtgggat acctgaacta
    15661 tccagcatag aaatagcatc acccgataac actataggat agggatgtcc gctccgtgta
    15721 taggttagct taaatccacc atctaaaagt ggagttatta atccatacaa tgcagtaaaa
    15781 taatatcccc caagaatagg aaataatttt tcattgagct gatgtataaa ttcatctggt
    15841 tggataattc cattattcca taaattttca gtagcatatt tcaaaaccga tgaaaacaat
    15901 gcggcaggaa cgccatgacc tgttatatca gccatgaata cggctaaatc atggttaatc
    15961 ttttttatag tataaaaatc gcctccaaca aaatcatacg gacgatagca ggtaacaatt
    16021 tttattccag gatattctgg gatgggggta ttaattattg attgctgcac cctttttgca
    16081 tattctaagt ccttgtttat aatatcattt cttttgcgaa gttcctcttc gcttttacgc
    16141 aatgcctcct cctgctcttt cttctcggtg atatcttctt tgatagctac aaaatgagta
    16201 acgttgccat gctcatccgc aactggtgca atgatacaat gttcccagaa atagcttcca
    16261 tttttttgtt tattatggaa aattccatcc catacctccc cacgtaatat tgtttcccac
    16321 atattttgat aaaattcctt tggctgatga cctgatttta aaatacgcgg attttggccg
    16381 atagcttcag taaaactata tccggtaata gattcaaatt ttggatttac atattgaatg
    16441 atccccctgg tgtcagttat gacaattgaa gccgggcttt gttctatggc tcgtattaaa
    16501 actgcctgat caatggctga taatgttttc ttcatgtata gttacctttt gttccttaat
    16561 cacacggcat tgcaccctaa ttcattatac tataaaaaat aatctttatg aaaaaaattt
    16621 attaacagta actatcaaac aaaaaaaata ctttaatgaa ttccttatta ccattgttac
    16681 cctattgatg tataatatta cacactcatt gcaatatata aacaatattt ttcaatgttc
    16741 tgaaagaaaa tatcttgacc ctttttctat cattacattt gatagtgcca tgagaaaaaa
    16801 taaccgccag catattcttg atactgcccg taaacttttc cttgaatatg gatataatgg
    16861 catatcaata cgcaacattg caaaacgggc aaagcttaca acaggtgcaa tttattttca
    16921 ttttaaaaat aaaaaagaga tatatagcac tatatgttta gaagctatca acacattgca
    16981 caggcttttt agtgatggca taaaactgcg taaaactcca gcacaaaagc ttatatcaac
    17041 atacgactcg tatttaaaat tttactatga atttcatgat tattacaatg tccttaccga
    17101 atacagagcc gaatatgacg atgagcctga cccctataaa gatgaaattc agaaacaaaa
    17161 tcttagtata caaaatttaa tggcagatgt atttaaagag ggcatcgatc agggtgtgtt
    17221 taaagacctt gacccaaaga tggtggcatt ttttctttca gcggttgctg aaggtatgtt
    17281 gcaatttaaa aagctgggtg taatggatgc cgcaggaatc accgacaaca aattcaggga
    17341 atttatgaag attatagtcg gacagggcat acaaaaaaaa tgaacacctt agcaaatcca
    17401 ctgacgatgt cacctgtaac attgctcacc tcacatctaa caccttacat tgtacactgt
    17461 acattttgca tattacattg catacctaac aactcaatat ctgtttttaa aatggcactt
    17521 tatgaatgct attgtttcat cttttttaac aatagatatc gctgcaactc gattatggca
    17581 gcagcaacaa tgagcatccc tgcaaaaaca aggattgttt tcacgatgct ccatccccac
    17641 agtattggtg cactcaccag aagtgtcccc aaaagtacat atggtgcacc aaatatactc
    17701 attataccag cttctatagc tgtggtggtt ttaaattctg gatcagctat tcttaaaagt
    17761 aacaaacctg ttgaaactgt acctgtcacc gtaccataga tcaacagcat cctttccaga
    17821 tttacgctcc ataacctgcg tccataatag taacatacaa tcgttgttat tattcctgct
    17881 aaaacacaaa tcactactat tggaacaata tattgccaca ccactacgac ctgaatagcc
    17941 ataactgttg caactaacag ataatccact gaaaacccgg tgatacgatg ctgaataccg
    18001 ggatcaataa gatacaatcc ccctaatttt gtaataataa atcttacaat gaaagctatc
    18061 aaaagcccga taaaaaagaa aaaaccccac atcatttgcc ccatctcagg gcttaataaa
    18121 ctgcttatcc cataaataac gacgtaggtg atcatataaa caacaccaac aagtgatgcc
    18181 tggaaagcaa gcgcatctac atttgatgaa tgcgtggtta attcacctgc cacctccttt
    18241 tcagattgct gagccataac acctcttcgt aattccattg ataactcttt gggagtgtac
    18301 actgccttac ctttgcgtat acctaaataa gcaagtggaa cacctataaa aaatgaaaac
    18361 atataaccag caacggcgaa ggttaaccct aatgtagcag aatgagcata tcccagattt
    18421 tcccacactt tcccaaccga aagcgcctgc ccgggacctt cttcaaatcc tatcggagtt
    18481 aaaaaaccaa atgaattaaa aagattatat cctaaataat tcaacaccat aacacacaaa
    18541 ccgcctataa ttgcctgtat agaaaatgaa actccctcaa tatttgccat ccataatgag
    18601 ccatgcagta cttcgccaac acctttatca tcagcctttt ccttattata ggtaagccca
    18661 attgatataa aagaaatatt aaaaagatga aatgcaagcg tttctaaaat atcggctgat
    18721 aaaggtataa tttcgtaact ccgaagaatt aaacctattg cgcccccgat taaacagctt
    18781 ggaattaaaa atctctggaa tgccttaacc tttgctctga ggataatgcc aagcaaaagc
    18841 attactgaca tccagccaaa tgcaatcatt gcctcaaacg taaaaggggt attcatatag
    18901 cgccctccat tttttatata tatttgtaaa agtttacaaa tgaaaaaagt atacgataat
    18961 gcacctttca gatgttcaga acaacctatt caaataatct tactgtgtaa taatactgct
    19021 gtcaatgtat ttattgctat aaatagcata cgtttgatac atcaaattta ttataatttt
    19081 ttattgatta ttttaaatta ccaattacaa tcacaacaac atttgccttt tagtaactat
    19141 cagtttgatg tactatttac aataaaaatc gcaatatcta tattctattg gaggttagtc
    19201 atgaaaatca ttgatctttc atccatcatt gaaccatcac catcaggaac acccgatttt
    19261 ttaaagattg atatcaaata tcattcgcat ggtgatggag caaaacaggt tgaggcactt
    19321 ttaaaggtac cagcccacct aatgcgtaat ggtgaaggct gggcaaccga aacaattacc
    19381 caattaggca cccatgatag cacccacgtt gacgctccat ggcactataa ctcaaccatc
    19441 cagggtaaac ctgcccctac cattgacgaa cttccacttg aatggtttta tggcaatggt
    19501 gtaaagcttg atatgaccca taaagcagaa ggtgaccctg taacacctca agatattgaa
    19561 aaagaattga aacgtattaa ctatactatt caacccaatg acattgtttt aatgtataca
    19621 gggatggaca aattttatgg acagcctgat tatattttca gggggtgtgg tgttacctat
    19681 gaagctactg tcatgctgta taatgctggt gttcgtgtta tgggcattga tgcatggggt
    19741 tgggatatgc cactaaatct tcaggcacaa aaggcattac aggaaaataa accgggcatt
    19801 ttctggtcag ctcatcaagc tgatttaata tactcgcata tggaaaggct tgttaattta
    19861 ggtttattgc cagcaaaagg atttaaggtt gcatgctttc cattaaacat caaacgtgcg
    19921 agtgctgctc ctgcacgggt tgttgctatt gttgaataat ataatttcca tataaattta
    19981 atattgacaa actatcgaac aaaatctggt atatataaag aacttataat tattaataat
    20041 taaataagtt catgcataac tgcaatccaa aacgatttta aatttcatgt taaataatta
    20101 aaatcactat ttatggatgc agtcataaaa acaattaata taaaagaggt aagctatggg
    20161 agatactaca caaaacaaat gtcattatat cattgaaaat ggaatcgcaa ttattacaat
    20221 tgataatcca ccaatgaatg cattaaacca tatcgtttta aaggatttac gtaattcagt
    20281 taatgcactt attgatgatg ccgccgtccg ggtaataatc ataactggtg ctggtaaagc
    20341 atttgttgca ggtgctgaca taaatgaaat caaagtgcaa aacacaaaag aagatggtgc
    20401 aaacttcctt aaaaacggtc aggaaatcta caacataatt gaaaatgccg ataagccatt
    20461 tattgctgcc attaacggat attgtctggg tggtggttta gaattagcgt tagcctgcca
    20521 tattcgtctt gctgatgaat ctgcacaagt aggtcttcct gaaattaaat taggtattat
    20581 tcctggatac ggtggtactc aacgaagtcc tcgtttattt ggtaaagcag cagcatatga
    20641 attaatcctt tcaggtaatt ttatttcagg aaaacaggcc gaaacatatc gtattgtgaa
    20701 cagggctacc caaaaaggaa cagttcttaa agaagctcaa gaattagcaa aagcaattgc
    20761 tgctcgcagc aggcttgccg ttaaagcagc catgcaggca atagcgcagg gatataccat
    20821 ggaattttat acagcactaa cctttgaacg tgagcttttt ggtaaacttt gtgagaccga
    20881 agataaaaaa gaaggcattg ccgcattcct tgaaaaacgc gagcctcaat ttaaagacaa
    20941 atagagggat ggacaacctt atcattcgtc atgctcatta tgatgagtta gaagcaattg
    21001 ctcgtttaga tgcacttgtt tttggtcaaa atgcgtggcc atttaatagt tttgtcaatg
    21061 aattgcagct tcatttttca aaattattaa tagctgaatt agatactaca tttgcaggat
    21121 ttgcgttagc gtggtatatt gccgatgaaa ttcaactgat gcgtattgct gtaaatcctg
    21181 tcttccagcg taacaaaatt ggttcaacac ttattcaaca tataactcat tatgctcaaa
    21241 accagagcaa aattgtttta gaggttgcag acaataatac acaagccata gcattttata
    21301 catcattagg tttttacaga gtaggcgtta gaaaaaatta ttatcattta aatagcgcta
    21361 ttttaatgga aaagattatt gatcatgaag attaatacag tacaatttat aacaagtgct
    21421 acaactccga atcactatcc aaaatattcg tttcccgaat ttgcatttat aggacgttcc
    21481 aatgtgggta aatcatcttt attaaatact attttaggca ggaaaaacat agcaaaaacg
    21541 ggtagtaaac caggtgtaac acaaacaata aatttttatt gcatcaatga tgccataagc
    21601 ctggcagact gcccgggatt tggctatgct aaaatttcac aatcaaagcg ccgtgaattt
    21661 ataacaatga tagataacta tattaatata cgatcgtcgt tgaaattagt ttttctttta
    21721 atagatatac ggcgtgaacc tgacgaacat gagattaata ttataaacaa atcgttacat
    21781 aaaaatatca atgttatgat tgtagcaact aaatgcgata aagtttcaaa aaacgaaatt
    21841 aatgcgggta tcaaaaggat ctgtaccagc ttaaatttgc atccggaatc ggttatacct
    21901 tcttcaaaaa caggcatggg taaaaatatt attttatcag ttattagcaa gcattgtcaa
    21961 taatgtgatg ctcatagctg tgtcatcaag tacatgaccc tgcttgcttt ttcttttggc
    22021 attaacgaaa gcaggtacgt tgaaatactc atccttcctt cttttgcagc attctggtca
    22081 atctggcgca atacatctat tatgagcgtt tcttcccatt gaaccataat atcaacagca
    22141 tcctgcgggg gaatgctttc aatctttttt gctaaatcca taacattctt tttatacccg
    22201 gaatacttct ttttttctaa ttccagagct ttcttttcct gatcaagccc tttctgtagt
    22261 tgttctaatt tttccttttc ttttgatatt actttttctg cctctgttat ctttgcttcg
    22321 cgcttatcca attcctctat tcgttgcaat aatttatcct tttctttttc aaactcttca
    22381 cgttctatca gcgatggttc atcatctgca gcatcaacaa cagatggagc ctctgtaaca
    22441 atatagtggt gatatatttt cccaagattt atcaagccaa tataatccat ccagaataat
    22501 ccaacaataa caatgaaaac tatcatcaat attaaataaa caacttttgt ggaatcagat
    22561 actaatttca tgttctactc ctcgtgaatt gctatcaaca ctgttactcc tcaatattaa
    22621 ataaatcccc cttgctggta tattgtgata tagctttttt cagcaaattc tgaatttgtt
    22681 gcatatcaga ggatgcctta ctatccgctt tattatacaa aacacttttc agtatttctt
    22741 ctgtaatttt attatcaaac gtattatttt tttgttcgat agttcctttt atatcaggtt
    22801 gttggttagt attttgctgc attgcattgg gagtattctg cactgttgtt ttttcagatg
    22861 gtgatattct attatatgtc ttttgtatcg cagcaaacct gctttttatt tcagctattc
    22921 ttttctgcat ctgttgtata ctgtttatgt ccatatatgc ctcacattgc attgtatatt
    22981 cgttgattta gatcatcaag ttcttttccc atttctctat taaactgata catatattct
    23041 tcatattttt tttcttttaa acgttcaacc acctttcttt cctttgaagc ttgcattaat
    23101 ttttgacgta ccctgtcaat ttcaggctgc atctgttcca ctcttttttt tgataagtct
    23161 atagcttttt gagcgttata agcaaatcgt tcgcacatta atgcttcatt atatgaaaag
    23221 gtaccgtccc tcattttttt tgaaagttta ttataatatt cttttatttt ttttctatac
    23281 tcttcctgca ttactatctc ttcgttttgt tttgctataa gcatggctag ctcagcttta
    23341 attgcatgtt cctttgaaat acgaatgtcc aataatgttt gcaatctgaa ctgaaatttt
    23401 ttcataacgt ataatctatt ttattaattt aacgattcct ttgtacaaaa tacggctctc
    23461 tcgatacaaa atctcttttt tgctcctgtt taacttttga accaaacatt aattctatta
    23521 atttcgtttt aatattttcg tatatatcct tttcataaat tccctgttta agaaattcat
    23581 ccattgacgg cttcatatct atagctgcat caacatctgg atctgaacct cttgcatagg
    23641 cacccagcat tattaaatct tcagcttcat gataagcagc taaaagtgtc ctgaatttgt
    23701 ttgccgcctt tttatgctct tcatctataa catcattcat acatcgggaa attgagccca
    23761 atacatcaat tgctggataa tgattcttat gagctaattt tctatcaagc acaatatgcc
    23821 catcaagaat accacgaacg gcatctgcaa tgggttcatt catgtcatca gcctcaacta
    23881 atatagaata aaaaccagtt atacttcctg cacacttttt attacctgct cgttcaagta
    23941 agcgcggaag taatgaaaac actgatggtg gatatcctct tgttgctgca ggttgcccgg
    24001 cagctaaacc aacctcacgc tgtgccatag caaatcgagt aatggagtcc atcaataaat
    24061 taacatgcaa ccccttatcc ctgaaatatt ccgcaactga atgagcaaga aacccggctc
    24121 gtaaacgcaa cattggaggt tgatcagatg ttgaaataat gacaactgat cgttttaatc
    24181 cttcattctg caattctttt tcaacaaaat cttttgcttc tcttccacgt tcccctatca
    24241 acgcaataac acttatatct gccgctgtat ttcgtgcaat cattgaaatt aatgttgact
    24301 tccctacccc tgaaccagaa aaaatgccaa ttcgctgacc tttcccaaca gttataagtc
    24361 catcgatagc acgtatcccg gtagacagcg gagaatcaat aatttccctt tctaacggtc
    24421 ttaacggttt attgtctaca ggataacctt cttttaaata aatattccct ttaccgtcta
    24481 taggattccc catcccatca ataacccggc caacaagttc atcaccaaca ggaacaataa
    24541 gttgtgatcc cactgcatac acatctgcac caggaacgac acctttcata tcaccaatcg
    24601 gcattagtat tattttattt ttattaaaac caactacctc agcatttaaa tatccgccgt
    24661 cttttaatct tatgcgacat aattctccat attgcacatc cggtccatgt gattcaatcg
    24721 ttaaaccggt aattttttca actcgccctg ttgccctgat tgtttctact tcttcaataa
    24781 cttctttgta ctttgtaaat atgtccacct tttcatgtgg tagtatctga atcattattg
    24841 cctcgttaat ctataattaa acataccatt ataatggtcg tgcatttcta attgcttctt
    24901 caatagcttc aagctgggtt gatatccgtg catctatcgc acctacatca gtttcaatga
    24961 tacacccacc ccgttcaaca cgcgagtcct catatatatt taccttttta agcgattcca
    25021 tcatcttgat taattcatct ttatgagcgg ttgtcatatc aaggtcagca aagttaacac
    25081 gtatatctat tctatcccta tctttaactc gttgcaatgc ttctttaata ttattaataa
    25141 caacctctct tctttccact atctcatctt tgatgacttt gcgtgctatc atcaagatca
    25201 tctctgtcat taatttttca gatgatcgta taatatcatc cctaatatca accgcagtag
    25261 atacaatagt tcccagcctg tcaataaggc gcattacttc agcctgccct tctttgtatc
    25321 cttcttctct tcctgcgtca taaccacgtt tatatgcttc atgctctatc tcagatacct
    25381 ttaattctgc ctcttttatc attttttcag cttcaaattt gccacgctca acttctcgct
    25441 cagattcaat ctttaagcgc tccatttcaa tttttatctg ttcacgtgcc tgctgtaact
    25501 ctaagaaggc cgctttttta ccatcctctt caatttgggc agctctggct ttagcttcat
    25561 caagcattcg acgcacttct tcttctgttt cacgtctata acgctctaat tctgcttcca
    25621 tctcttcaat agatggaccc tgataaactt ccacgggatt tccttgctca tctacttcaa
    25681 attcagcata ttcttcatca gttagtatat ttttctgata tttatcgggc aattcaatct
    25741 gtttgggggt tgcagtaatt tgaatttcag caggtttaaa tacaagctta gacaacgagt
    25801 tcctcctcgc cagctcgtgc tacaacaata tcgccagctt cttctaattt tcttattata
    25861 ttgactattt tttgctgagc ctcttcaaca tcacgtaatc taataggacc cataaagtcc
    25921 atatcttctc ttagaagagc agaagcacgc tttgacatat ttctgaatat cttttcctgt
    25981 acttcagcat ccacaccttt taatgctttt gccagatcct gagtatcaac ttcacgcaat
    26041 actttctgaa ttgctctatc atcaagaaga acaatatctt caaatacaaa catcctcttt
    26101 ttaatttctt cagctaattc tggatcctct tcttccagtg cctcaataat aatcttttca
    26161 gtaccacgat caacatgagt caacacctca actattgaat caatcccacc tgcagaggta
    26221 taatcctcgc tggctaatgt agacagtttt cgctccagca ctctttcaac ttcacggaga
    26281 acatcaggtg aagttctatc catagttgct atccgccgcg ctacatctgc ttgaatttga
    26341 tgatttaatc cagataaaat agttgcagct ttttgcggat ccaaatatgc cagtatcaaa
    26401 gcaatagttt gtggatgttc accctgaata aagtttaaga gatgacttgg gtcggtcctg
    26461 cgtataaaat caaaaggcct aacctgcaat gaggaagtca aacgattaac aatatcaata
    26521 gctttctgag ttcctaacgc acgctcaaga acctcgcgag cataatcaat tccacctgta
    26581 gcaatgaatt cctgagccat cataagctcc tggaattcca ttaaaacttt atctctatct
    26641 tcaggctcta ctttatctaa acgtgctatc tcaaaggtta actgctctat ttcatcttct
    26701 ctgaggtgct taaatatttc agatgataca tctgatccta acgacaccag aaatatcgca
    26761 gccttttgtc tgcctgttag ctgtgacttc ttttgctgta acatattcta tctccgaatt
    26821 ttgccgatat actgttccca aatcattacc tatctgtctg attatagtat ataccccatt
    26881 ctttcaaaag tcaatacacg ctttttagta tatcggccta tggctattaa ccaataacaa
    26941 cattattctt ctgcaagcca tgtcctcaac agctgcgcca catcatccgg tcgttcacgg
    27001 gctaaattaa cggcattctg ctgcaactct aaccttgcct tttcttctaa tgacaattca
    27061 acatcaattc cttcttcttc agcagcacgt agggcagctt cccgcatacg ctgctgctct
    27121 aatgctaatt gttcctcgcg tatacggcgt ctgcgtgcaa gttcacggga tatagccctg
    27181 aaaagtatca cgcctaaaaa caacgccatt ataccaacca gcgctgccaa aagcatgcgt
    27241 ttaaactgct cttttttcct aaattcctca cgagcaatat cccattgttt tgttctatca
    27301 aacataatat tttcaacagc cacctgatca ccacgtgcag cattaaaatt aatagctttc
    27361 tttacaatat tttcagcctg ccgtagctca tcctcgctca acggtagctg aacaggtttt
    27421 ttagcaggat caagatcata gctgccatca gggagacgtg gtaaatcctg ataaccatca
    27481 atagctattg caactgatac tgctgaaatg tcaaaaggat ctctctctat tttacgaata
    27541 gttttattga tcgcattatt ttcaacattt tcttctttat cgtattgcga aaattggtca
    27601 tcacttgctt tatatcctgg tggtttatta ccttccgttc cagcaggacc ttccggattc
    27661 caaccatgtc cttgaaaatg ttctttagta ctcttttttg aaatagtcaa agcatcttta
    27721 acctttcgtt cagaatacgg cgttagtgga ttatcctttt ccatctcaat aggagtatat
    27781 tcttcctgtt cttcctgaat tttatcccag ttaaaatcca tgttaagcct tactatctgt
    27841 atacgatcag aagaaaaaat cttttccagt cccagacgta tatcctttaa catctggaca
    27901 cgtgcttttt cttctatttt ttttctatat tcaagaaggg taaattcctc ttttgcctta
    27961 tcaaattggt catcaaaatc cgagattatt tttccatatt catctgttat agatacattt
    28021 tccggcttta atttagtacc aactgctctt gatactaaat actgtatacc tttgatttct
    28081 tttttagaca gagtatcata cccgggtgca aggtaaactg taacagcagc cgtataggca
    28141 ttttcttttt ctgtaaaaag ctcatcttcc gtaatagcta tttcaacgtc agccttttca
    28201 atatttttta atgattcaat gtgacgttta atttcatccc gcaatgccct catatattta
    28261 acattcagct ctttatcagt ctcggtccat tttgatatat caaaaagttt ccagccaggt
    28321 atccctcttg gtatcatatt ctcctgtgca agacgggtta aaataacttc acgctgattg
    28381 ggatctacca gaatagtggt tgttcctgat gttgtatagt ggaaacccat ttcttccaat
    28441 ttttttgtta cctgcccaaa ttcatcagaa ggaagatcag agaataaaac tatatttgct
    28501 ttctgtgacg acaccgagaa taaaacaata aatgcaataa caagaacccc tgttaccaca
    28561 ccaataataa ttttttttgt tgtatctaat ttagcataaa tctctttcaa ttgttgaaat
    28621 atcttggtaa ataaatccat atcttcttcc ctcccacaac taattaaata tcatcacctt
    28681 gctccacaat aaccatgctt cacattacaa aatgattatt actgcaataa catcatcaac
    28741 atatcaattg actgcaaagt gacataatac atatagcatt cacacataat aaaacatcag
    28801 tattaaaaag tcaattaatt tactaaaatt tgctactttt tttattatat tcacagcaat
    28861 cacatattat ctgagattaa tgagttccct gtatgccctg atcgcttcat cccggacagc
    28921 ttttgttaag cttaatgcaa tctcagcttt ctgttgagca atcatcactg tatgtatgtc
    28981 tactgactca ggctcataaa tcatctgttg tgtcaaatct tctgcatcaa cctgtaaatc
    29041 atttaccttc cccaatgttg atttcagcgc atccataaaa gaacctgcaa catcatcgtc
    29101 ctgaattttt tctggtttat tgtcataatg gagaggatgc gttgtcttca ttgtaaaaat
    29161 atttgattta cttataaaat tactttcctg taccataatt aacgctccct gaaattattc
    29221 ttgattcaac aatttgtcgc tcaccaatac caaactgcag ccattccttt tttcgtctgc
    29281 ttaacgtaat gggagtcgat agttcatgtg aacaacaaat ttcatttata tgcccttttt
    29341 caaaatacat atcttaaact ccttccgtaa aaattataca ccacccttgc caatttccag
    29401 cgcctttgaa aacatggatt ttgcattatt aatcatcatg acatttgctt catatgaacg
    29461 cgatgcagag attaaatcag tcatctccat gaccacatta acatttggca tttc
//
LOCUS       DAOSOX010000012        27648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1740_length_27648_cov_8.655094, whole genome shotgun sequence.
ACCESSION   DAOSOX010000012 DAOSOX010000000
VERSION     DAOSOX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27648
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1740_length_27648_cov_8.655094"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..430
                     /locus_tag="PLX22_01830"
     CDS             242..430
                     /locus_tag="PLX22_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18674.1"
                     /translation="MNRVITIFNSFEEAENNEVEYWKSLDPNKKLTILEEIRQRHMEI
                     TGEGKQGFQRVIKIIKQK"
     gene            378..665
                     /locus_tag="PLX22_01835"
     CDS             378..665
                     /locus_tag="PLX22_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18675.1"
                     /translation="MKVSKDFKELLKLLNKNNIKYITAGGYALGYHSRPRYTEDIDIW
                     IEASYENAEKNILDVEALFATNLPMNREYIQHQLAELHITPHQKIINIILQ"
     gene            complement(652..3009)
                     /locus_tag="PLX22_01840"
     CDS             complement(652..3009)
                     /locus_tag="PLX22_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOT18676.1"
                     /translation="MKKLRFYILWLLIIILNTVNSYATISSVVLTPSNPTFLSMGNGY
                     YLAGKTYTFTAQVVDPDVTAWNQITEVVLTIPNSTNIQLRVSAITGTGAGNHTLTIVS
                     GIAGCSSSIAGTWNNFTVTFTVTFQWNTQESAWALNRSVVASATTTVPANNTLTDTKL
                     VSYGICASIRILNFAQDGVSADAMINPWHSAFNVTGTIVYNVPGAAALDAVHLIADAG
                     AGEITNAELRRNGISTVTNDGDETDGLTLTVGAEYFYNLDTTPPYSQLGNHTWTVRIT
                     CATAGGPEVSNNSLTVTCDLVEITNIEIINGGGVNASPDYWRSVNVPGTQVRVTARMQ
                     WAGTNVVGNTTVRMQDSEGNNTDVLIPNGQPNAVANVTYPTVLAQVTDTITYQAITVS
                     GGAYDDEQNVAARIPQPVTRTVYWENADPPGANTPPFTPFSGSSSTAFTITVNWVPLS
                     NPIDGDFDSYRIYYKENSSATWLLLDRTIVAALGNRATGSATITSLKSLTDYDIRISA
                     VDVFGYEVAVANQITTAIATTAGSLEVSITDGITEWGDTTFGTLASNRPLRRTAIRVT
                     INITTDSPDAVNLIVAAGDIGVGVDLIVGGVINPALTENTDYYRIAATRTTSTGWVSY
                     IPDTNPLITLGTDVRFIVETIKSGVASYSDSDSDTEVPPGNPNDLEYSFTVAMQPQFT
                     PWPTRILNNVITNKNPLCYPAYYLTDDALVTITVYDIKGRAVVKLLDKAFRKGGQNIK
                     EGGWNGDNKAGNKLGVGLYYVHIRAKRVSDGKVILNSFQKVVIAK"
     gene            3123..6998
                     /locus_tag="PLX22_01845"
     CDS             3123..6998
                     /locus_tag="PLX22_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT18677.1"
                     /translation="MKKNTLVMTALLACIVSFFIQCSNEAPRVFPAPPGGSGGTGIGI
                     DAPDFLGSYPENGDTSIPVDTSVVLVFSEPVDLGTIVPGNVDISGGVTYSASASSDGR
                     TIILTITSGNLSANTAYTVDIDTGILDQDTPQRNLITSYSFTFTTSADTSTFELPRVV
                     AASRYPAPLATNVSINQTFVDVTFTKPIDDTTVVANASFDITPGSGTGITFSPNRQTF
                     RLNLATPLAYGTVHTINLTAAIEDLFSNPLVLDGNHTWTFTTEAAPASGALAISNVWV
                     TDITDTTAILHWTTSIAQAAISIDYGLTSAYGSFASEPAGTRTVHQVSIGGLTLSTKY
                     YFRIQYGAVTATGSFITADNTATSNDKSITDTAGDKSLLTIAQNQTIISVSPYYTQDG
                     SSFIAWKDPDGTVYASYIDNDAAPTKRWGADGSAVDSNNRTVLRVFSDFMGYCFIASG
                     DGTNVYLKRIYNSAGALTFDSTFGANAAAIGLSISTGTNPAVTMLWGNQTANNVSAGF
                     VAKVVPAGAVPNTTEMNIPWANYFFDYDVDLSGVATGNYIIDGSYNQTTVNVTGQNYR
                     HVLGTAANVITATETYRIADNTASTAFTATDHVVNNTTAGTQTPYSNSGTNAYTNHGD
                     NLTGWGTNDLITDGTNWGYLTANAGEVLLTTNLTDSGVSDGFFSFDNYLIRSNTSPVY
                     QYSLVKNEDVPAYSYIANPVVTTETWLPLNDDIFNSTSENYSIWNGTYCWTHVQDPPF
                     DSFYTISFLPSGLISAGETVTLYSYITGGTADTPRTNPLYDDGVDLSAVALNDIVVNL
                     SAYTYSRANNIAYSSAGALGLLANIMSNSQLYWILRFVNPAEPILVIGQATTTSVSQL
                     ISTAKSAGFSGIVEKGDLIYNITDNRYAIVTNVDDNDTLTLNKDIITINDYFIIFASN
                     EPLIESGTVTTTGNPFTDTNANFTSANGAVRGGDIVHNLTAGTSAYVVAVNSATQITL
                     NNNIMTAAGDRYVIIQPRVLVAYERTGDIYGKVIRLRDGSTYLNEFTICNAAGTQANV
                     QLITSGYTSTNPGAIALYRSGAGPTYTYYAKRINGAGVINAADNAANGGLGVQATPAN
                     ATLLAALSDDAGGMFVLYKITNDIYVRRILSTMAISWSNTINNVVDAAICRSATGVIV
                     TYNKTTNSSQLFAQRYTGAGVVEFGETTIVALTPYAYTTNLTITPDRNDGAIISWIDE
                     RYLSQLGYTVMAQAINATGTPQWDADPGAGTDYDGVLIGITNIWYKPYSGLKAAFYND
                     GAPPYGGIFIWYDRRNNKQDIYYDTQPYP"
     gene            complement(7052..7258)
                     /locus_tag="PLX22_01850"
     CDS             complement(7052..7258)
                     /locus_tag="PLX22_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18678.1"
                     /translation="MLAENNRTSINKTILSLIHKSPGLEERTSRMRDSGDIAGTWSDE
                     EFRGFSLLMKDFEKIDKEVLISGI"
     gene            7752..9761
                     /gene="uvrB"
                     /locus_tag="PLX22_01855"
     CDS             7752..9761
                     /gene="uvrB"
                     /locus_tag="PLX22_01855"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT18679.1"
                     /translation="MPPFKVISSYTPSGDQANAIEQLADGINKGLKFQTLLGVTGSGK
                     TYTMAKVIEKIQKPTLVLTHNKTLAAQLYREFKEFFPENAVEYFVSYYDYYQPEAYVV
                     SQDLYIEKDASINDEIDRLRLKATSSLVERQDVIIVSSVSCIYGIGNPESFEKNHIRL
                     ATGHEVDRQQLLMKLVSIHYDRNDIAFTRGTFRVRGDTVEVFPANLQYALRIVFFGDE
                     IEALYKINPLTGNRIESITQAHIYPAKHYVSSKEEMEETIRMIQIELEHRINEFTSSG
                     RLVEAQRLETRTLYDIEMLRTVGYCNGIENYSRIIDRRQPGQPPHCLLHYFKGNYMMF
                     VDESHVTIPQVRGMYEGDRSRKSNLVEYGFRLPSALDNRPLQFHEFETMIDRVVFVSA
                     TPAEYELQISAQVVEQVIRPTGLIDPEIEVRPATGQVDDLIGEIKKRVAVNERILVTT
                     LTKKMAEDLTQYLTEVGLRVRYLHSEIETIERVEIIRDLRKGIFDCLIGINLLREGLD
                     IPEVSLVAILDADKEGFLRSTRSLIQTSGRAARNSNGKVIMYADTITGSMQEAIDETS
                     RRRKLQMEFNTLHNITPKSISKEIVDIIEREYHSDDVFADLVSEYANAYRTNNGEQLK
                     MVHAKIKQDMLEAANNFEFEKAAVLRDQMFDIEKKIDIVSKASGR"
     gene            9785..10624
                     /gene="nadC"
                     /locus_tag="PLX22_01860"
     CDS             9785..10624
                     /gene="nadC"
                     /locus_tag="PLX22_01860"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT18680.1"
                     /translation="MEQPSYEELSPIITMALQEDIGSGDITSLALFDEYQYACARISS
                     KNDGIFCGGFLIEYIYHTIDPAISVTIYCNEGAAIKKDDVVCDIEGSVRALLSAERTV
                     LNFLQRTCGIATRTARLVSLVSDTPIKIFDTRKTVPGFRLLDKYAVYCGGGTNHRMGL
                     FDMILIKDNHIKAAGGIQQAVMMVRQKYANRYTIEVEVETLNQVHEAVEAGVDIIMLD
                     NMTKDRISLALSIINGKAKVEVSGNMDEHTLKEIADLPVDYVSVGALTHSVKAFDLTM
                     RLI"
     gene            complement(10636..11475)
                     /locus_tag="PLX22_01865"
     CDS             complement(10636..11475)
                     /locus_tag="PLX22_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOT18681.1"
                     /translation="MIVGIVGAGTMGTDIAHLAIEHNYHVIVHDTDETQLKLAYEIIT
                     KRLDRYIAEKRLEQTKRGEFISRLKLHKNIKDMRNADIIIECIPEDIALKREVFKALD
                     AVCKSGAILASNTSSLSVTTIAAATKRPEYVVGIHFMNPARVMRLVEIVGGIATTRET
                     IESVKSFVKSLGKTPVESKDFPGFVLNRILMPMINEAIFTLYEGAANAETIDKTMTLG
                     LNLPMGPLQLADMIGLDVVLAITDELYKGFGDPKYRPCPLLKKYVEAGYLGRKAGRGF
                     YTY"
     gene            complement(11486..12451)
                     /locus_tag="PLX22_01870"
     CDS             complement(11486..12451)
                     /locus_tag="PLX22_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT18682.1"
                     /translation="MYTIVFLDAATVDFGDIDLTPLSQAGRLITYSITKPEEVIERSK
                     AADIIITNKVVFTRKEIECLEHCKLIAVAATGYNTIDIEAAHSRGIAVANVPGYSRES
                     VAELTMCFILSLACNLINYNEATHDGSWSNSPIFTMGKWPTMCISGKVLGIMGYGDIG
                     KRVAQLAAVFGMRIIAFKRGNALSGDSSIPRYTADEFCKLSDFITIHMPLNDYTYHCI
                     NKDFILKMKPTAYLINMARGPIIDTKDLAWALNNNVIAGAALDVLEKEPPDIDDPILK
                     APHCIITPHIAWASKETRQVLINEIAANIHAFFNGKQRNIVNATV"
     gene            complement(12612..13091)
                     /locus_tag="PLX22_01875"
     CDS             complement(12612..13091)
                     /locus_tag="PLX22_01875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT18683.1"
                     /translation="MVQVHLLNHFPQYAPILAYWSYREWYTQRDIPFDVIIRSYMERA
                     HSSTIPLAWVAIDEKKLPVGMVSLKNNDLWARTDLNPWLASLYIVPEYRFQGIGTALV
                     QAVIDKAKELGYRTVYLFEGHNDTVDLIHYYTSRGFCILEDALDNDGNPTTIMYSNV"
     gene            complement(13158..14786)
                     /locus_tag="PLX22_01880"
     CDS             complement(13158..14786)
                     /locus_tag="PLX22_01880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOT18684.1"
                     /translation="MKRFIEENNKEQFDVIIIGGGITGAAVAYEAATRGLHVALLEKK
                     DFGWATSAATSKLIHGGLRYLANMEISLVRESLRERRILENIAPTLVYPFPFMIPNYK
                     RLLNNRWVLKMGMIMYDILSFDKGWTWDRSKKIPVHSQISREKALQEEPNLKSEGLTA
                     ATIYYDCQSIFPERLTLSFIKSAVNYGAKVANYAEVKDFLYRNNSIAGVIVYDTLNNK
                     EIDVEGKLVINCGGPWADIILKKAENGTVKHQIRRSEGIHIITKPIVHKYAVTLMSKK
                     GRHFFLIPWRGYTLIGTTDKEYIGNPDNYRVTRESIQELLDDVNEYFGDGTLSYNDIK
                     YTYGGLRPLVDDQVEGTYQSSRRYEIYDNSEEGVQGLITVEGGKYTTSRNLAVKVMKV
                     VEKKLQYSLPKSITHKQYLAGCEIKDLEIFFNTVYTSNSLLDKKSLEVLAKYFGTEVD
                     TVIKIAGSNKKFREIVTHDGEILAEVIYAIRHEMARTLPDIVFRRTGICTAGNPGNDK
                     LTLVAETAAKELRWDNKELSKQLEEVKQRLQIPK"
     gene            complement(14791..15507)
                     /locus_tag="PLX22_01885"
     CDS             complement(14791..15507)
                     /locus_tag="PLX22_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOT18685.1"
                     /translation="MKKNTLGQLQKNTLREQIVTLLAKKIINNAIQPGDKLPSERDIA
                     ADLDVNRATVREALKKLETLGLIEIRHGEGIFVNDYRSSGNLELFRMMIYLNDTIPLP
                     ILQDVLEMRKIIVPEMAARAVKECSTSDLEELARIINEQEDILEKDLAVHHFIAKTSN
                     NMLYIFILHFFNQIFRDFGYLYFENPDNRKRSEQFHQQLYKAFQDKKAEKAFSITRDV
                     LEYTEKRIFEYYTKTYSNKG"
     gene            complement(15607..16782)
                     /gene="galK"
                     /locus_tag="PLX22_01890"
     CDS             complement(15607..16782)
                     /gene="galK"
                     /locus_tag="PLX22_01890"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOT18686.1"
                     /translation="MPSVVDTAITHHKEYFPQSSDVSVFVAPGRINIIGEHIDYNGGT
                     VFPIAIDKHIYICASRNNDGIMRFASYQQHEQGEIAISNIHLPNIRNVAKHKWLVYPL
                     GACVLSDIHFESGFDISFYSTLPVGAGMSSSAAITVGTLYALCTLHGKKMKNIDVALQ
                     AQQVEWDFAGVQCGIMDQMAIVNGRKNNGLLLNTKTMEFQYIPIDAFAIRFVLVNSGV
                     KHSLRESGYNERRNECERALTLLQKSGFAINCLCDCSIDELATIKDILPITEYKRVFH
                     VISENHRVHEFAQEIQKYNLIKAGKLLYQSHHSLSNYFEVSIPLIDAMVEWISEIDGV
                     YGSRLMGGGFGGCTVTMVALYAVEHFKKTIAERFEKKEGRTPDIYECTIDDGVHQLL"
     gene            complement(16788..17651)
                     /locus_tag="PLX22_01895"
     CDS             complement(16788..17651)
                     /locus_tag="PLX22_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18687.1"
                     /translation="MKHSADVITHRFKSSINTLALYALEHELSNDLIASEIDKRFNNV
                     KSIKKDKIMHDVLQSCYKLVWNVNNTFEAGFAAKKIQNDYSECLNNCNAIIIARCILN
                     ILKNTNQKPKKNERLSADFYSQCVNSLLSNPLLQYDSDLLTIYCKQTVIILKVAGIIN
                     ENKIMEINSDDLYRQMFLAFWNKCNWKSLFPSGGYISNEIKNSRQLLLDIIVNTNKSV
                     QLDEVAQTFFELTGIAKYNDLFAVSLLDFSVITWLSFFGIIRYTENSFDKPVTIALTQ
                     HAFPLLQFLLC"
     gene            18373..19296
                     /locus_tag="PLX22_01900"
     CDS             18373..19296
                     /locus_tag="PLX22_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOT18688.1"
                     /translation="MWLLSLIDAAQNEIKEIQNNTKCILHVLSEEIGERTLRKYDNLN
                     KSRQFIYNYLHQSNDNVYEEKYLVDNLEVSNIIAEIPGFEHSNEIIIIGAHYDTIEDT
                     SGADDNGSAVAGLLELHRMLSQYTFRKTLRFVAFTLEEPPFFSGPYMGSMVHALGCKD
                     KGENINLMICLEMIGYAGNRMQQDYPLRSMQNEYPPYGNFLAVVSLPSYAPYAYKWKK
                     EYNKYAKNKIVDMIGPASIPGINLSDHYSFNKFGFPAIMITDTAFYRNKNYHTEGDTY
                     GTINFKFLSESIFNTFMAVKTLSNERDILDE"
     gene            19289..19828
                     /locus_tag="PLX22_01905"
     CDS             19289..19828
                     /locus_tag="PLX22_01905"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT18689.1"
                     /translation="MNEFKPLPIQDSHRLINAGNVILISAQSKEKKTITTVAWHMPVS
                     NNPKLLSCALANKRYSLELIKESKSFCINIPDFAMLDTVIFCGTYSGRTVDKCKEAHL
                     TALPCSDIDGFFIAECVAYIECQLYSMVEAGDHTIVIGQVTCAHAKIHCFTPDGVIDI
                     NKVQLIHHLGGTHFGILKN"
     gene            19842..20045
                     /locus_tag="PLX22_01910"
     CDS             19842..20045
                     /locus_tag="PLX22_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOT18690.1"
                     /translation="MECKKDRNLKVCTCTYEPCSRKGLCCDCISYHLKSRELPACAFS
                     KEAEKTYDRSFEHFVKLVSAGKI"
     gene            complement(20340..21227)
                     /locus_tag="PLX22_01915"
     CDS             complement(20340..21227)
                     /locus_tag="PLX22_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOT18691.1"
                     /translation="MYSWMSAFIITLFILVSISCVPPAYRSKPDTSRDKHNYYKFEQP
                     EEYTPFQDDTMYIQKNKKDTTMPVCYDKYSKYEEDDTKHIMSDSYQHINPVPENNSKS
                     GIWHTVKKGETLYRLSKKYAVSINDIQTMNNIANPTAIKAGQRILIPISDDSADCGIN
                     KQSKISKGATKKSISKPLFMWPVTYVAGVGRDNKNGARPIGILIKSTPKSNVMASAHG
                     TIVKIGSMRGFGNFVVIQHKKDFYTIYSKLDAIRVKEGQVVGCGTTIGILDATNPSLH
                     FQINQSGKPLDPLMYLAKK"
     gene            complement(21335..21754)
                     /gene="nikR"
                     /locus_tag="PLX22_01920"
     CDS             complement(21335..21754)
                     /gene="nikR"
                     /locus_tag="PLX22_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOT18692.1"
                     /translation="MNSVIRFGVSIDEKLLDRFDALINEKGYVNRSEAIRDLIRDMLV
                     KEDVSDPNSEVIGTLTLVYSHDVREIADKLNDLQHDNYKYIISSVHVHMDEHNCLEVL
                     LMRGTAQQVKKIADNLLAIKNVRHGKLTVTTTVDYHE"
     gene            complement(21898..23442)
                     /locus_tag="PLX22_01925"
     CDS             complement(21898..23442)
                     /locus_tag="PLX22_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT18693.1"
                     /translation="MKLFILIFYGLILLLLSIFGAHRYYTMYLFIKHSRNKVKPKAVF
                     KNLPKVTVQLPIYNEQYVVERLINAVTQLEYPRKKLEIQVLDDSTDETSALAEKICKQ
                     KRKEGYTIIYIHRTDRTGFKAGALENGLKYTDSEFIAVFDADFMPNPDFLQKTIHYFT
                     DPTIGMVQTRWDHINRHYSLLTECQSILLDGHFMIEHTARNRSGRFFNFNGTAGVWRR
                     QAIIDGGGWQHDTITEDLDLSYRSQISGWKFLYLPDVTVPAELPIEISAFKAQQFRWA
                     KGSIQVFKKLYKTILNAQLPLKIKIESFMHLGANFAYLLMVFLTLLMPVALWVRYYGG
                     FKKFAYLDLPIFILATLSVVIFYFYTELSILYHNKNSFDSKFKPLVYLPMVLAVGIGL
                     SINNSKAVIEALFNKKSDFNRTPKYNVASLHRINAVEHVKTLLKNKYSLHTINIVSII
                     EFILGLYMSLAVYFAFDKNLYVSIPLIMLFQVGYLYTSLFTIINSITHHIIVIANSLR
                     RGVVND"
     gene            23597..24247
                     /locus_tag="PLX22_01930"
     CDS             23597..24247
                     /locus_tag="PLX22_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18694.1"
                     /translation="MTQATIQALQGLRDLSMIKWHVIPLLAIVFYIYTREMKEARTSG
                     NWNAIFAGLTIFGVDFFNETWNGWVMHLTQRSAVWTTPGDTALRTMVGWNIEIMFMFS
                     ILGIIFYHTLTGKNIKILGIPEQWFWAIGYSAFCVFVECLLNVGGHLVWEYEYWRLSF
                     KGVWLIFLFGYFHFFFFANIVIGLSTIKKKIIAVGIIYAVPIIMNIIALGFLGWMY"
     gene            complement(24267..25592)
                     /locus_tag="PLX22_01935"
     CDS             complement(24267..25592)
                     /locus_tag="PLX22_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOT18695.1"
                     /translation="MAQKEDIVFPKGFLWGTATAAHQIEGGTTNNNWWLFEKEGTIKN
                     NDSALVACDHWNRFKQDFDLLKKLNCNAYRMSIEWSRIFPQPGTLDKKALDHYVTMIN
                     ELLKRKITPFVTLFHFTLPIWWCNEGGLLNRDKEHLEHFKQFCDVVAKTLKGKVKFYN
                     TINEPSIVMLGYITGTSAPGQKNMLKAIKALGTTMLMHAIAYHTIKSVDPDAKVGIVH
                     NVQITKPYNPNSIMDKLFTAFADYCFNGAIIRSLKKGKLPLQIFSSHRELINTNDFIG
                     VNFYNFIRLSLRLKDLAVPATDNPVCGKKGLCVSLGWEPYPEGFYLAIKLMHNAFSHI
                     PMYITENGIGTNDDSWRQRVLIDHLKMLHKAITEGVDIRGYFYWSIIDNFEWEHGYEP
                     RFGLVHVDFKTQKRTIKHSGMVYAQIAKSNALPATILKKFPEEIYKPQF"
     gene            26011..27357
                     /locus_tag="PLX22_01940"
     CDS             26011..27357
                     /locus_tag="PLX22_01940"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOT18696.1"
                     /translation="MTVKNHSVKENYMPTTIKNLGPCTVVSPLAVKYRQREAAFINDA
                     IRVRFNAFIGKKPPSGGETIESFEAAGPREKIFFNPETTRVAITSCGGLSPGINDVIR
                     SLVMESYYGYGINDIIGIPYGYNGLNPEYNYTPIPLNPDNVKSIHMQGGSILGSSRGG
                     TRDMDKLVDTLQKLNVSILYTIGGDGTLRGSHDIAMIALQRGLPLAAVGIPKTIDNDI
                     SFIQRSFGFETAFSIAVEAIYAAHTEAIGAPNGIGLVKLMGRHSGFIAANAALASNDV
                     NFVLLPEVPFELYGANGLCAHLENRLQRRGHAVICVAEGAGQEILQKDSDKQYDASGN
                     VKLADIGVYLKEKIQEYFKQKEIEINLKYIDPSYMIRSAPAVPNDSIYCTLLGQNAVH
                     AGMAGKTDLVIGQWSNIYTHVPVEVAISKRKRIDPHSRFYSSVLDATGQPFAMINK"
     gene            27384..>27648
                     /locus_tag="PLX22_01945"
     CDS             27384..>27648
                     /locus_tag="PLX22_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18697.1"
                     /translation="MPKEHKNAPLICAVITGIAIAGAGIGIGKSMPLIIALGIIPAIV
                     YEIYRTQGMFTTLASWLALIVTIAALYVIYKNTMIDIAPYISKF"
BASE COUNT         8420 a         5526 c         5241 g         8461 t
ORIGIN      
        1 aacgagtata taatttagat ctgtcaaaat ttggaggcta tatctttcac attaaacaca
       61 atgcagcaca ctgggaaaac gatatcgacg gtcctaaaga cttgaaaacc ttcagagcac
      121 atataaaaaa ataaagcaaa acattttgtt gatagttacc cataaacaat tggtgcaata
      181 gcatcatcat tgtgttgtaa ttgtagttga cattcacaca aataagatgt acacgttaag
      241 catgaatcgg gtaataacaa tattcaatag ctttgaagaa gccgaaaata acgaggttga
      301 atactggaaa tcattggatc caaacaaaaa gttaactatc ttagaagaaa ttagacaacg
      361 acacatggaa ataaccggtg aaggtaagca aggatttcaa agagttatta aaattattaa
      421 acaaaaataa tataaagtat atcactgctg gtgggtatgc tttaggttat cattcacggc
      481 cacggtatac tgaagatatt gatatatgga ttgaagcatc atacgaaaat gcagaaaaga
      541 acatacttga tgttgaagca ttgtttgcta caaacctacc catgaacagg gaatatatac
      601 agcatcaatt ggcggaattg cacattacgc cacatcaaaa aataataaac attattttgc
      661 aatgaccact ttttgaaatg aatttaatat caccttgccg tcgctgacgc gctttgcccg
      721 tatatgaaca tagtatagcc caacccccaa tttgttccct gctttgttgt cgccattcca
      781 gccgccttct ttaatgtttt gaccaccctt tctgaatgcc ttatccagca gttttacaac
      841 agcccgtcct tttatatcat acaccgtaat ggttaccaat gcatcatcgg taagatagta
      901 tgcaggatag catagtggat ttttattcgt aatgacattg tttaatatgc gggttggcca
      961 tggagtaaac tgtggttgca ttgctactgt aaatgaatac tcaagatcat taggattgcc
     1021 tggcggaact tctgtatctg aatcgctatc tgaatatgag gcaacccctg atttaatagt
     1081 ttcaactata aaacgcacat ccgttccaag cgttatcaag ggattggtat cggggatata
     1141 gctaacccaa ccagtagaag ttgtcctggt tgcagctata cggtaataat ctgtattttc
     1201 agttaatgct ggattaataa cgccaccaac aattaaatca acccccaccc ctatatcccc
     1261 tgcggcaaca ataaggttaa ccgcatccgg tgaatcggtt gtaatgttta ttgttacccg
     1321 tatggctgtt ctgcgcaacg gcctgttgga agccagcgta ccaaaggttg tatcacccca
     1381 ttcggtaata ccatcggtaa tggaaacttc caaacttccc gctgttgttg ctattgcagt
     1441 agtaatctgg ttggccacag caacctcata accaaaaaca tcaacggctg aaatccgtat
     1501 gtcgtaatca gttaacgatt ttaaggaagt gatggttgca ctgccagttg ctctattgcc
     1561 caatgctgct actatagtcc tgtcaaggag cagccatgtt gctgaggagt tctctttata
     1621 ataaatcctg taactgtcaa aatccccatc aatcggattg cttaacggta cccagttaac
     1681 agtaatggta aatgccgtgg aagatgatcc actaaatggt gtaaatggtg gggtgttagc
     1741 tcctggcggg tcagcatttt cccagtaaac agtccttgtt acaggctggg ggatacgcgc
     1801 tgctacattc tgttcatcat cataggcgcc cccgcttacc gttattgcct gataggtaat
     1861 ggtatcagtt acctgcgcaa gtactgtcgg gtaggtaaca tttgccacag catttggctg
     1921 accattaggg attaatacat cggtattatt cccttcgcta tcctgcatcc ttactgttgt
     1981 attgccaaca acattggtac ctgcccattg catccgtgct gtaaccctga cttgagtccc
     2041 cggaacgttg acacttcgcc agtagtcagg agaagcatta acccctccgc cgtttataat
     2101 ttcaatattg gtaatttcaa ccaggtcaca agtaacagtc agggagttgt ttgaaacttc
     2161 cggaccccct gcggttgcgc aggttatccg gaccgtccat gtatgattgc ccaattgact
     2221 atagggaggt gttgtatcca gattataaaa atactcggct ccaaccgtta atgttaatcc
     2281 gtcggtttca tctccatcat tggtaaccgt acttattcca ttgcgtcgta attcggcgtt
     2341 ggtaatttca cctgcgccag catctgcaat caggtgcaca gcatctaatg cagcagcgcc
     2401 cggaacatta tatacgatag ttcctgtaac attgaatgca ctgtgccatg gattgatcat
     2461 tgcatcagca ctaactccat cctgtgcaaa atttaatatt cggattgatg cacatattcc
     2521 atacgaaaca agttttgtat cggttaatgt attatttgcg ggaactgtcg tggttgctga
     2581 tgctacaacg cttctgttta atgcccatgc tgattcctga gtattccatt gaaacgtaac
     2641 agtaaatgtt accgtaaaat tattccatgt gccggcaatt gatgatgaac accccgctat
     2701 cccggaaact atcgttaagg tatgattacc ggctcctgta ccagttattg cggaaacccg
     2761 taattgtata ttggtggaat ttggtattgt cagtacaacc tcggttatct gattccatgc
     2821 tgttacatca gggtcaacaa cctgagcggt aaatgtatat gttttccccg ccagatagta
     2881 gccatttccc atagataaaa aggttgggtt ggatggtgtt aaaacaacac tgctgatagt
     2941 tgcatagctg ttaacagtat ttaagattat gattaacaac catagtatat agaatcttag
     3001 ctttttcata tcatatgtaa agaaaattaa tttgcacttt tttttataat atcatatatt
     3061 agctaaaaag gtcaatattt ttattgtact ttttataatg acctttactg tgacgatagt
     3121 tcatgaaaaa aaatactctt gtaatgactg ctttattagc ctgcattgta tcctttttca
     3181 tacaatgttc taatgaagct cctcgtgtat tccctgcccc tcctggcggc tcagggggta
     3241 ccggtattgg tatagatgct cctgattttt tgggctcata ccctgaaaat ggcgacacct
     3301 ccatcccggt ggacacttcc gttgtccttg ttttttcaga acctgttgat ctgggtacga
     3361 tagttcctgg taatgttgac atttctggcg gcgttaccta tagtgcatca gcctcgtctg
     3421 atggcagaac aattatttta accattacat caggcaatct ttcagccaat acagcatata
     3481 ccgttgatat agatacaggt atccttgatc aggatactcc tcaacgcaac ctgataacca
     3541 gttatagctt taccttcacc acatctgcag acacttcaac atttgaactt ccacgggttg
     3601 tagctgcatc ccgttatcct gcaccattag ctactaatgt aagtatcaat caaacttttg
     3661 tggatgtaac ctttaccaaa cctattgatg atactaccgt agttgcaaat gcaagttttg
     3721 atataacacc cggttcagga acaggcatta ccttttcacc gaaccggcaa acgttcagat
     3781 taaaccttgc aacaccgctc gcctatggga cagtgcacac tatcaattta accgctgcaa
     3841 ttgaagatct attcagcaac ccattggtcc ttgacggaaa ccatacatgg acctttacca
     3901 ctgaagctgc tcctgcaagc ggtgcgctgg ccataagcaa cgtatgggtt actgatatca
     3961 ctgataccac cgctatactc cactggacaa caagcattgc tcaagctgct atttccattg
     4021 attatggact aacttctgct tatggatctt ttgcatcaga acctgcaggg acccgcactg
     4081 tgcatcaggt atcaatagga ggtttaacac tttcaaccaa atattatttt agaatacagt
     4141 atggcgcggt cactgcaaca ggcagtttta tcactgcgga taatacagca acatccaatg
     4201 ataaaagtat taccgatact gctggagata aatcattatt aactatcgcc caaaatcaaa
     4261 ctattatttc tgtatcacca tattatactc aggatggctc ttcattcata gcatggaagg
     4321 accctgacgg caccgtatac gcctcatata ttgataacga tgcagcccca accaaacgct
     4381 ggggtgctga tggcagtgcg gttgattcca ataatagaac cgtactgcgg gttttttctg
     4441 attttatggg gtactgtttt attgcatcag gagatggaac aaatgtatac cttaaacgta
     4501 tttataacag cgcaggtgca ttgacatttg attcaacctt tggcgcaaat gcagcagcaa
     4561 tcgggttaag catttccact ggcaccaacc ctgccgtaac aatgctatgg gggaatcaga
     4621 ctgctaacaa tgtcagcgct ggttttgtag caaaagtggt acctgctggc gccgtgccaa
     4681 acacaaccga aatgaacatc ccgtgggcta actatttttt tgattatgac gttgatttaa
     4741 gcggtgttgc aacagggaat tatattatag atggttctta taatcaaacc accgttaatg
     4801 taaccggaca aaattatcgt catgttctgg gtaccgcagc taatgtgata actgcaactg
     4861 aaacataccg cattgctgac aatactgcca gcactgcttt taccgccacc gatcatgtcg
     4921 taaacaacac aacggcaggt acacaaacac cgtacagcaa ttccggcacc aacgcctata
     4981 ccaatcatgg cgataatctc accgggtggg gtaccaacga cctgattact gatggaacta
     5041 attggggata cttaacggca aatgctggtg aggtgttact cacgactaat ttaaccgatt
     5101 caggtgtttc tgatgggttt ttcagttttg acaactattt aatcaggtcc aacacttcac
     5161 cggtatacca atacagttta gtaaaaaatg aagatgtccc tgcgtattct tatattgcta
     5221 atcccgtggt aacaactgag acatggctgc cactgaacga tgatattttt aattcaacat
     5281 ctgaaaatta ttcaatatgg aatggaacct attgctggac acatgttcag gatccaccct
     5341 ttgattcatt ttataccata tcattccttc cttcgggtct tattagcgcc ggtgaaacag
     5401 ttacattgta ttcctatata actggtggta ccgctgacac tccaaggaca aatccactgt
     5461 acgatgatgg tgtggattta tccgctgttg ctcttaatga tatcgttgtt aatctttccg
     5521 cgtataccta ttcacgggca aataatatcg catacagctc tgctggtgca ttaggacttc
     5581 tggcaaatat catgtccaat agccagcttt actggatttt acgctttgta aatccagcag
     5641 agcccatact tgttattgga caggcaacga caacgtctgt cagccagctt atatcaacag
     5701 caaaatctgc tggattttct ggtattgttg aaaaaggtga cctgatctac aacataactg
     5761 acaaccgtta tgctattgtt accaatgttg atgataatga cacacttacc ttgaataaag
     5821 atataatcac aataaatgat tattttatca tctttgcaag caatgaaccg ttgattgaaa
     5881 gcggaacggt taccacaaca ggtaatccat ttaccgatac caatgcaaac ttcacttcag
     5941 ccaatggggc tgtccgtggt ggtgatattg tccacaacct tactgccggt accagtgcct
     6001 atgtagtagc tgtcaactca gctacccaga ttacacttaa caataatatt atgacagcag
     6061 cgggagaccg ttatgtcatt atccagcccc gcgtgctggt tgcctatgaa cgaactggtg
     6121 atatttatgg taaggttatc cgattacgag acggtagcac ctatctgaat gaatttacca
     6181 tctgcaatgc tgcagggact caggccaatg tacagcttat cacatcgggg tacacaagca
     6241 ccaatcccgg agctattgca ttgtaccgga gtggcgcagg gccaacctat acctactacg
     6301 ctaaacgcat caatggagcc ggtgttataa atgcagcaga caatgcagca aacggtggat
     6361 tgggagtaca ggcaacacct gccaatgcta cactgcttgc tgcactatcc gatgatgcgg
     6421 gcggtatgtt tgttttatat aaaattacaa atgatatata tgtccgtaga attttatcca
     6481 ccatggctat ctcatggtca aatactatta ataatgttgt tgatgctgca atatgcagat
     6541 cagcaacagg tgttattgta acgtataaca aaacaaccaa ttcaagtcag ctttttgctc
     6601 aaaggtatac cggtgccggt gtggttgaat ttggtgaaac aaccattgtt gctctgactc
     6661 cttatgctta cacaaccaat ctaacaatta cccctgatag gaatgatggt gccatcataa
     6721 gctggattga tgagcgttac ctgtcacaat taggctatac tgttatggca caggctatca
     6781 atgcaacagg cacaccacag tgggatgctg atccaggtgc cggtaccgat tacgacggtg
     6841 ttcttatagg catcacgaat atatggtata aaccctattc aggattaaaa gcggcatttt
     6901 ataatgatgg cgcaccaccc tatggaggta tattcatatg gtatgacagg cgcaacaata
     6961 agcaggatat ctactatgat acacaaccat acccgtagaa tacttttatc tgcatataaa
     7021 acgaaactat acaaactttg ctctgacttt tttatattcc actaatcaag acttctttat
     7081 ctattttttc aaaatctttc atcaagagag aaaatcccct gaattcttca tcactccatg
     7141 tcccggcaat atcgccacta tccctcatcc ttgaagttct ctcttcaagc ccaggagatt
     7201 tgtgaataag ggaaagtatt gttttgttaa tgcttgttct gttattttct gcaagcatac
     7261 acaactgatg atatgttctt tttggcattt gtctaagagt aatttgattc attatatgct
     7321 tctatgatat cattctatga tactatcata actattcttt aagtgtttat caaataattt
     7381 acttcattta gatagtattt tatatttttg tgccactaca tagtaaaacg cattgtatgg
     7441 cttgatgata ttctccatgg aatatatgta agtgtttacc atcatctgtg gctggattat
     7501 gtattaacgg tgcttctata gccagtaact ttctgtcatg ctccatgaca atcaacagaa
     7561 tccatcatat cacgttacgc aagttgctgc tggatttacg tttgctgtaa ccagagtgca
     7621 taaaaatgaa ttagtgcaca tgcaatggta tatataaaaa gtaactatcg cccaaaatct
     7681 tttttgattt acctgaaatt agtattatgt tatgttgtac atagctatca ttatcatgag
     7741 ttttatacac tatgccacca tttaaagtta tttcatcata tacaccttca ggcgaccagg
     7801 ctaacgccat agaacaactg gcagatggga ttaacaaagg tttgaagttc cagacgcttc
     7861 ttggtgtcac cggctcgggt aaaacctata ccatggcaaa ggtcattgag aagattcaaa
     7921 agccaaccct ggtactgaca cataacaaaa cactggctgc acaactgtac cgcgagttta
     7981 aagaattttt ccctgagaat gccgtggaat actttgtatc atactatgac tactaccagc
     8041 ctgaggcgta cgtagtttcc caggacttgt atattgaaaa agatgccagc atcaacgatg
     8101 agattgatcg cctgcgttta aaagcaacct catcactggt ggagcgccag gatgtgatta
     8161 tcgtgtcatc ggtttcgtgt atttatggta ttggtaatcc cgaaagcttt gaaaaaaatc
     8221 atatacggct tgccactggt catgaggttg acaggcaaca gcttttaatg aaacttgttt
     8281 ccatacacta tgaccgtaat gacattgcat ttacccgtgg aacattcagg gtcagaggcg
     8341 ataccgtgga ggttttccct gctaatctac aatatgcatt gcgcattgtc ttttttggcg
     8401 acgaaattga ggcactgtat aaaataaatc cgttaaccgg caaccggata gaatctatta
     8461 cgcaagcgca tatctaccct gcaaagcatt atgtttccag caaggaggag atggaagaga
     8521 caatccggat gatacagatt gagctggagc atcgcatcaa cgaatttacc agcagtggca
     8581 gattggttga ggcacagcgg ctggaaaccc gcacgctcta tgacatagag atgttgcgca
     8641 ccgtgggata ttgcaacggc attgaaaatt attcacgcat cattgatcgc agacagcccg
     8701 gtcagcctcc gcactgtttg ttgcactact ttaaaggcaa ctacatgatg tttgttgacg
     8761 agtcacatgt caccataccg caggtacggg ggatgtatga gggcgataga tcccgtaaaa
     8821 gtaatctggt tgaatatgga ttcaggcttc cttcagcact ggataaccgc cccttacagt
     8881 tccatgaatt tgagaccatg attgacaggg ttgtgtttgt ttccgcaacc cctgcagaat
     8941 atgaactgca aatatcagcg caggttgttg agcaggttat acgccccacc ggtttgatag
     9001 atcccgaaat agaggtgcgc cctgcaaccg gtcaggttga cgacctgatt ggcgagatta
     9061 aaaaaagggt tgcggtaaat gaacgtatac tggtaaccac attgacaaaa aagatggcag
     9121 aagacctgac acagtatctt accgaggtag gattacgggt tcgctatctg cactcggaga
     9181 tagaaaccat tgaacgcgtt gaaattatcc gcgatttgcg gaagggcata tttgattgtc
     9241 ttattggtat taaccttttg cgcgaaggtc ttgatatccc cgaggtttca cttgttgcaa
     9301 tcctagatgc cgataaggag ggatttttac gctcaacacg ctcgcttata caaacatcag
     9361 gcagagcagc acgcaatagt aacgggaaag tcatcatgta tgccgacacc attaccgggt
     9421 caatgcagga agcaatagac gaaaccagca ggcgccgaaa actccagatg gaatttaata
     9481 ccctccacaa cattactcca aaaagcattt ccaaagagat tgttgacatt attgagcgtg
     9541 aatatcattc tgacgatgtt tttgccgatc ttgtatcaga atatgcaaat gcgtaccgca
     9601 ccaacaatgg tgagcaactg aaaatggtac atgcaaaaat taaacaggac atgcttgaag
     9661 cggcaaacaa ttttgaattt gaaaaagctg ctgttttgcg agaccagatg tttgatatag
     9721 aaaagaagat cgatatagtg agtaaagcat ccgggcgata gatactatat aagggtatga
     9781 tactatggaa caaccatcgt atgaggagct atctccaatt ataacaatgg cattacagga
     9841 agatattggc agcggtgata taacaagcct tgcactattt gatgagtacc agtatgcctg
     9901 tgccagaatt tcatcaaaaa atgatgggat attttgcgga gggtttttga tagagtatat
     9961 ttatcacact attgatcctg ccatttcagt gacgatatac tgcaatgaag gagctgcaat
    10021 aaaaaaagat gatgttgtgt gtgatattga aggcagtgta cgggcattac tttcggctga
    10081 gcgtaccgtt cttaactttt tacagcgtac ctgtggcatt gcaacacgga cagcacgcct
    10141 tgtttcactg gtcagtgata cacctataaa aatatttgat acacgcaaaa cagttcctgg
    10201 gtttagatta ctggataaat atgccgttta ttgcggcggg ggaaccaacc accggatggg
    10261 actttttgat atgattttga ttaaggataa tcatatcaag gctgcaggcg gaatacaaca
    10321 ggcagtaatg atggtacggc agaagtatgc aaacaggtat accattgagg tagaggtaga
    10381 aacgctgaat caggtacatg aagcggtaga ggctggtgtt gatatcatca tgctcgacaa
    10441 tatgacaaaa gacaggattt cactggcatt atccatcatc aatggtaagg ctaaggttga
    10501 agtttccgga aatatggatg agcatacgtt gaaagagata gctgatttac cggttgacta
    10561 tgtttcagtt ggcgcattga ctcattcggt caaagcattt gatcttacca tgcgtttaat
    10621 ctaataaaat ctaatttaat aggtgtaaaa tcctcgtcct gcttttcttc ctaaatatcc
    10681 tgcctctaca tattttttta acaacgggca cggacgatat ttgggatctc caaatccctt
    10741 atacagttca tcagtaatag caaggacaac atccagtcct atcatatcag ccagttgcag
    10801 cggtcccatg ggcagattca atcccaatgt catagtttta tcgatagttt cagcattcgc
    10861 cgccccttca tacagagtaa atattgcttc attaatcatt ggcataagaa ttctgtttaa
    10921 tacaaatcct ggaaaatcct ttgattcaac aggtgttttc cccagagatt taacaaagct
    10981 tttcactgat tcaatggttt cacgagtcgt agcaatacca cctactatct ccaccaatcg
    11041 cataacacgg gcgggattca taaaatggat cccgacaaca tattcaggac gtttagtagc
    11101 tgctgcaata gtggtaacag acaatgatga tgtattactt gctaaaatag cccctgattt
    11161 gcatacagca tccagcgctt taaatacctc tctcttcaat gcaatgtcct cggggataca
    11221 ttcaataatg atatctgcat tgcgcatatc ctttatgttt ttatgcaatt tcagacgtga
    11281 aatgaactca ccacgttttg tttgctctaa acgcttttcg gcaatatatc gatcaaggcg
    11341 ttttgttata atttcataag caagttttaa ctgcgtctca tcagtatcat gcactatgac
    11401 atgatagtta tgttcaattg ccagatgagc aatatctgtt cccatggtac ctgcacctac
    11461 aattcctact atcatagaaa cctccctata cagtggcatt cacaatattt ctttgtttac
    11521 cattgaaaaa tgcatggatg tttgctgcaa tctcattaat taaaacctgc cgagtttctt
    11581 tagaagccca ggcaatatga ggggttatga tgcaatgtgg cgctttaagt atgggatcat
    11641 caatgtcagg tggctctttt tccagtacat ccagtgctgc accggcaatg acattattgt
    11701 tcaatgccca tgccagatcc tttgtatcaa tgataggacc ccgtgccata ttgataagat
    11761 atgctgttgg tttcatcttc aaaataaaat ctttattgat acaatggtag gtataatcat
    11821 tgagtggcat gtggatagtt ataaagtcgg atagtttaca aaattcatca gcagtatagc
    11881 gtggtataga actatcacct gacaatgcat tgcctcgttt aaaagctata atccgcatcc
    11941 caaagacagc ggctaactgt gcaacacgtt taccaatatc accatagccc ataatgccca
    12001 ataccttgcc agaaatgcac atagtaggcc actttcccat ggtaaagatt ggtgagttgc
    12061 tccagcttcc gtcatgggtt gcttcgttat aattaatgag attgcatgcc agcgacagta
    12121 taaagcacat ggtaagctcg gcaacagatt cacgggaata gcccggaaca tttgcaacag
    12181 caattcctct gctgtgtgct gcctcaatat caatggtatt gtatccggta gccgccactg
    12241 caataagttt acagtgctcc agacactcta tttctttacg tgtaaaaacc accttgttgg
    12301 taataatgat atccgcagct ttactacgct ctattacctc ttcaggtttc gttatggaat
    12361 aggttatcag ccttcctgct tgcgaaagtg gtgtcagatc aatatcgcca aaatcaaccg
    12421 tggcagcatc aagaaacact atcgtataca cggatatcca tcctccaaaa aaaagttatg
    12481 tgcccttgca ttttcattcc ttttgagcgt ctgcaatata accatcgtaa aatcccagaa
    12541 aataacatta cattaggaca ggctaagatt ttaaataaag cgcaattatg gattaaatgt
    12601 caatagttta tttatacatt actatacata attgttgttg gattgccgtc attatcaaga
    12661 gcatcttcta agatgcaaaa cccgcgtgaa gtataatagt gaatcagatc aactgtatca
    12721 ttatgccctt caaagagata tactgttcta taccctaatt cctttgcttt atcaataact
    12781 gcctgtacaa gagcagtacc tataccctga aagcggtatt caggaacaat atataaagag
    12841 gcaagccatg gattgagatc ggttcgtgcc cacagatcat tgtttttcaa tgaaaccatc
    12901 cctaccggaa gttttttttc atcaatggca acccatgcca gtggaatggt tgatgaatgc
    12961 gcacgttcca tgtacgaacg gataataaca tcaaacggta tatcacgttg agtataccac
    13021 tcacgatatg accaataggc taaaataggg gcatactggg gaaaatgatt caatagatgt
    13081 acctgcacca ttgttgccat ccttattgat tgatagttat agacatgaat agcaacaatg
    13141 gttgttgttc gcaactacta tttgggaatc tgtagccgct gcttcacttc ttccagttgc
    13201 tttgacaatt ctttgttatc ccatcgcagc tcttttgctg ctgtttcagc caccagtgtc
    13261 agtttatcat taccgggatt tccagccgtg caaatacctg ttcgcctgaa gacaatatca
    13321 ggcaacgtac gagccatttc atggcggata gcataaatta cctcagccag aatttcccca
    13381 tcatgggtta ctatctccct gaacttttta tttgacccag ctattttgat tacagtatca
    13441 acttcagtgc caaagtattt tgccagcacc tcaagggact ttttatccag caaagaattt
    13501 gaggtgtaaa cagtattaaa aaatatctct aaatctttta tttcacaccc tgctaaatac
    13561 tgtttatgag ttatggattt tggtaacgaa tattgtagtt ttttttcaac aaccttcatt
    13621 accttgaccg ctaaatttct gctggttgta tatttcccac cctcaaccgt tataagtccc
    13681 tgaacgcctt cttccgaatt atcgtaaatt tcatatcgcc gtgaagattg gtaggtacct
    13741 tcaacctgat catcaaccag aggtcgcaag cctccatagg tatactttat atcattatac
    13801 gataaagtac catcaccaaa atattcatta acatcgtcaa gtaattcctg aatactctct
    13861 ctggttaccc gatagttatc gggattacca atatattcct tatcagttgt gccaataagg
    13921 gtatatcctc gccagggtat caaaaagaaa tgcctgccct ttttgctcat caacgttaca
    13981 gcatatttat gtactatagg ttttgtgatg atatgaatcc cttctgacct tctaatctgg
    14041 tgttttacag tgccattttc agcctttttt aagattatat ctgcccatgg gccaccgcag
    14101 tttatgacaa gctttccttc aacatctatt tctttattgt tcaaagtatc gtaaacgata
    14161 acaccagcga ttgagttatt acgatacaaa aagtctttta cttcagcata atttgcaacc
    14221 tttgccccat agttcaccgc agattttata aatgatagtg ttaaccgttc agggaagata
    14281 ctctggcaat catagtagat agtagccgca gtcagccctt cagatttcag attgggttct
    14341 tcctgtaatg ccttttccct gctaatttga gagtgtacgg gtattttttt tgatctatcc
    14401 catgtccagc ctttatcaaa tgaaagaata tcgtacatga tcatacccat ctttagaacc
    14461 cagcggttat tgagcagcct tttataattg ggtatcatga atggaaaagg gtacaccagc
    14521 gtaggggcaa tattctctaa tattcttcgt tcacgtaagg actctcgcac cagggaaatc
    14581 tccatgttcg caaggtatcg taatcctcca tggataagct tggaggttgc agccgaagta
    14641 gcccatccaa agtctttttt ctccagcagt gcaacatgta aaccccttgt tgcggcttca
    14701 tatgccacag cagcaccggt aatgccgcca ccaatgataa taacatcaaa ctgctctttg
    14761 ttattttctt caataaaacg tttcatagca ttacccctta ttggaataag ttttagtata
    14821 atattcaaaa attctttttt cggtgtactc cagcacatct ctggtaatgg aaaatgcctt
    14881 ttctgccttt ttatcctgaa atgctttata taattgctgg tggaactgtt ctgagcgttt
    14941 tcggttatca ggattttcaa aatacaaata gccaaagtct ctaaaaatct gattaaagaa
    15001 atgcaaaatg aatatgtaga gcatattatt gctggtcttt gcaataaaat ggtgcactgc
    15061 aagatccttt tccagaatat cttcctgttc attaattatc ctggctaact cctccagatc
    15121 gctggtgcta cactccttta ccgctctggc tgccatctct ggtacaatga tctttctcat
    15181 ctccaggaca tcctgtaata tcggcaaagg aatagtatca ttaaggtaga tcatcatcct
    15241 gaaaagttct aaattaccac ttgagcgata atcgtttaca aaaatcccct caccatgtcg
    15301 tatctctata agccccaatg tttccagctt cttcagcgct tcacgtactg ttgccctgtt
    15361 aacatccagg tctgcagcaa tatcgcgctc ggaaggcagt ttatccccgg gctgtatagc
    15421 attgttaata atttttttgg caagcagggt aacaatctgc tcccggaggg tgtttttttg
    15481 caattgccct aatgtgtttt ttttcatact gccaccttta gtttagtggt tcagtggttc
    15541 aaccactatc aatataccag atatatagca aaaaatccac attaattgaa aaaaaataaa
    15601 tgatttttat agcagctggt gtacaccatc atctatggtg cattcataga tatcaggggt
    15661 ccttccctct tttttttcaa atcgttcagc gatagttttt ttaaagtgtt caactgcata
    15721 cagagctacc atcgttaccg tacaaccacc aaaaccgcca cccataagcc gtgagccata
    15781 cacgccatca atttctgata tccattcaac catcgcatct ataagaggaa tactcacctc
    15841 aaagtagtta ctcagactat gatgcgattg ataaagaagc ttaccggctt ttataagatt
    15901 atacttctga atttcctgtg caaactcatg tacacgatgg ttctcagata ttacatgaaa
    15961 caccctttta tattcagtaa tgggaagaat atccttgatg gtagcaagct catcaatact
    16021 gcaatcacaa agacaattaa tggcaaatcc agacttttgt aatagcgtca gcgccctttc
    16081 gcattcattc cttctttcgt tatagcccga ttcccgtaaa ctatgtttta cgccgctatt
    16141 cacaaggaca aaacgtattg caaatgcatc aatcgggata tactgaaatt ccatggtttt
    16201 ggtgttgagc aaaagcccat tgtttttcct gccattgacg attgccattt ggtccattat
    16261 cccacattga acaccggcaa aatcccactc tacctgttgc gcttgtaacg ctacatctat
    16321 atttttcatt tttttgccat gcaaagtaca taacgcatac agcgttccaa cagtaattgc
    16381 cgcagagctt gacatgcccg caccaacagg cagggtgcta taaaaactaa tatcaaatcc
    16441 agattcaaaa tgtatatctg acagtacaca ggcacctaaa ggatatacca accatttatg
    16501 ttttgccaca ttcctgatgt ttggcaagtg tatgttgctg atagctatct ccccttgctc
    16561 atgttgctgg taagaagcaa agcgcatgat gccatcatta ttgcgtgatg cacatatata
    16621 tatatgttta tcgatagcta ttgggaaaac ggtgccacca ttataatcga tatgttcacc
    16681 gataatattg atacgccctg gagcaacaaa tacactgaca tcagaagatt gaggaaaata
    16741 ttctttatga tgtgtaattg ctgtatcaac tactgaaggc atagacacta acacaacaaa
    16801 aactgtaaaa gtggaaatgc atgctgagtt aatgcaattg ttacgggttt gtcaaaagaa
    16861 ttctctgtat aacgtattat gccaaaaaat gataaccatg taatgaccga aaaatcaagc
    16921 aatgaaacag caaacaggtc gttatatttt gcaatcccgg ttagttcaaa aaatgtctgt
    16981 gcaacctcgt ccaactgtac tgatttgttt gtgtttacaa tgatatccaa tagtagctgt
    17041 cggctatttt taatctcatt tgagatgtat ccacccgatg gaaaaaggct tttccagtta
    17101 cacttattcc agaaagctaa aaacatctgc cgatatagat cgtcactgtt tatttccatt
    17161 attttattct cattaataat accggcaacc tttaatatga tgacagtttg tttacagtat
    17221 atagtaagta aatcactatc atactgcaat aagggattgc tcaataaact attgacgcat
    17281 tgtgagtaaa aatctgcaga tagccgttca tttttttttg gtttttggtt tgtattttta
    17341 agtatgttga gtatacaccg tgcaataatt attgcattac aattatttaa acattcgcta
    17401 taatcatttt gtattttttt tgctgcaaac ccagcttcaa aggtattgtt aacgttccat
    17461 accaatttat agcaggattg aagaacatca tgcataattt tatctttttt tattgatttt
    17521 acattattaa accgcttatc aatctcgctt gcaatcaaat catttgacaa ttcatgttca
    17581 agtgcataca gtgcaagcgt atttattgat gatttaaacc gatgcgttat cacatcagca
    17641 gaatgtttca tagttatata ctactccctc aaaaatggaa taataccgtg tcattcttaa
    17701 tgagcaaagc aactgaagaa tcttgttgtc catattgtca ttctgagggg caaaaccaca
    17761 aagaatatat tctttcataa atgacaagat tcttcgctaa cgcttagtat gacataccct
    17821 tacaacgact gcatgcattg cttcaactta gctcagcaca ggcacttttg ctttagaatg
    17881 gctataggct tcaccataag aatgccaata atgcttcatt tccatcatat ttaggaagat
    17941 gccctgtagc cataaggaaa tccactgtta aaaatatttt aaataaaatg taccaaaaag
    18001 gaataaaaca atacaatata tggttacatc agcttatgaa attaattgat aaacatacta
    18061 tcatgaatta atttttagtc aattaatatt ttattaaaat aaaaaaaagt tgtaaaggta
    18121 aaaaaatact gtacatttat ttacttcact aatattaata tattaaaaat ttttggtttt
    18181 tacttgcatt aataaaatga ttttttttat taatattaat gtgtaacatt atttaaacaa
    18241 gggaacctct aataattata ttctgaatgt ttccttgtga acacaagcat tgacagcatg
    18301 ttttttattt tttaataacg aaaaacttga tttttaaggg cgttcataaa tataaaaaca
    18361 agaggtccgg ggatgtggtt attgtcttta attgatgcag cacaaaacga aataaaggaa
    18421 attcagaata atacaaaatg cattttgcat gttttatctg aagaaattgg cgagcgaaca
    18481 ttgcgaaaat atgataattt aaataaaagc cgccaattca tatacaatta tttacaccaa
    18541 agcaatgaca atgtatatga agaaaaatat ctggtagata atttggaagt aagcaatatt
    18601 attgctgaaa taccaggttt tgaacatagt aatgaaataa taattattgg cgcacattat
    18661 gataccatag aagacacttc cggtgctgat gataatggat ccgctgtagc cggtctgctt
    18721 gaactgcata ggatgttatc acagtataca ttcagaaaga cattgcgttt tgttgcattt
    18781 actcttgagg aaccaccttt cttctcagga ccatatatgg gcagcatggt acatgcgctg
    18841 ggatgtaaag ataaaggtga aaacattaat ttaatgatct gccttgaaat gataggatat
    18901 gcagggaaca gaatgcagca ggattaccca ttaaggagta tgcaaaacga atatccacca
    18961 tatggaaatt ttttagctgt ggtttcatta ccaagctatg caccgtatgc atataaatgg
    19021 aaaaaggagt acaataaata tgcaaaaaat aagatagttg atatgatagg accagcttcc
    19081 atacccggaa taaatttatc agatcattat tcatttaata aatttggatt tccggcaata
    19141 atgattactg atacggcatt ttacaggaat aaaaactatc atacagaagg cgatacctat
    19201 ggaacaataa attttaaatt tttatcagaa tcaatattta atacattcat ggcagtaaaa
    19261 acgctatcaa atgaaaggga tatccttgat gaatgaattc aaaccattac caatacagga
    19321 ttctcatcga ctcattaatg caggcaatgt tatattgatt tcagctcagt ccaaggaaaa
    19381 aaagactata acaaccgttg catggcatat gccagtaagc aataatccaa agttattatc
    19441 atgcgcactg gcaaataagc gatatagcct ggaattaatc aaagaatcca aaagcttttg
    19501 tataaatatt cctgattttg ctatgcttga tacagttata ttttgtggga cttattccgg
    19561 acgaacagtg gacaaatgta aagaagcaca tctgactgcc ttaccatgtt cagacattga
    19621 cggatttttt attgctgaat gtgttgccta catagaatgc cagctttatt caatggtgga
    19681 ggctggtgat catactatcg ttattggcca ggttacatgc gctcatgcaa aaatacattg
    19741 ctttacacct gatggtgtca ttgatattaa caaagtgcaa ttaatccacc atttaggcgg
    19801 aactcatttt ggaattctaa aaaattagga cggataacga tatggagtgt aaaaaagatc
    19861 gaaatctaaa agtatgcacc tgtacttatg agccttgctc acggaaagga ttatgctgtg
    19921 attgtatttc atatcacctg aaaagcaggg aactccctgc ttgtgccttc tcaaaagaag
    19981 cagaaaagac atatgaccga tcatttgaac attttgtaaa actggtttca gcagggaaga
    20041 tataacatat gtattcttct atacatagca ttattcacca gtgtcattga gggcttgacc
    20101 aggaatctaa ataattaact gatatcctga aatgaattca ggatgacact ctgcacattg
    20161 ttattccagg cttacttctg catcaaaatg actgtcgcca gctatttcca tcccctatgg
    20221 aggtcataag ccagccatgg gtaactcctc agaaaataga aattttcatc taatcagcag
    20281 ttgaaaaata attgtaccag gattttgtgg gacaaaatat tacaatttta gatataccat
    20341 tacttctttg ccaggtacat caaaggatcc agtggtttcc ccgattgatt aatctggaaa
    20401 tgaagtgaag gattggtagc atcaagtatc ccgattgttg tcccacaacc aacaacctgc
    20461 ccttccttaa cccggattgc atcaagtttg gagtatattg tgtaaaaatc ctttttatgc
    20521 tgtataacaa caaaatttcc aaacccccgc attgaaccaa tttttacaat ggtaccatgt
    20581 gcacttgcca taacattgct ttttggagtg cttttaatga gtataccaat gggtcgcgct
    20641 ccatttttgt tatcacgccc aacaccagca acatatgtta ccggccacat aaacaatggt
    20701 tttgatatac tttttttagt agcccccttg ctgattttgc tttgtttatt tattccacaa
    20761 tcggcgctgt catccgatat gggtatcaaa atacgctgac ctgctttaat ggcagtagga
    20821 ttagcgatat tgttcatagt ctggatgtca ttaatggaaa cagcatattt ttttgaaagt
    20881 ctatataatg tttcaccttt tttaacagta tgccatatcc cggatttact attattttca
    20941 ggtaccggat taatgtgctg ataactatcg ctcatgatat gtttagtatc atcttcttca
    21001 tatttactat atttatcata acacactggc atggttgtat cttttttatt tttttgaata
    21061 tacatagtat catcctgaaa gggggtatac tcttcaggct gttcaaactt atagtaattg
    21121 tgcttatcac gggatgtatc gggtttagat ctatatgcag gtgggacaca actaattgaa
    21181 accaatataa atagtgttat aataaaggca ctcatccatg aatacatgga acatctcctt
    21241 atgttccgtt tgttagcaca ttaccgggaa gtaatagtaa cccttatcat tatatcggat
    21301 agttattctg cagaattgag taattaatta ttttttattc atgatagtcg acagttgttg
    21361 ttacagttaa cttgccatgt cgaacatttt ttatagccag caaattatca gcaatttttt
    21421 taacctgttg agcggtaccc cgcattaata aaacctctaa acaattatgt tcatccatat
    21481 gaacatgtac tgatgaaatg atgtatttat aattgtcatg ttgcaggtca ttgagtttat
    21541 cagctatctc acgaacatca tggctgtaaa ctaatgtcaa tgtcccgata acttcactat
    21601 tggggtcgga aacatcttct ttaacaagca tatctctgat taaatcacgt attgcctctg
    21661 aacgattgac atatcctttt tcattgatta aggcatcaaa cctatcaaga agcttttcat
    21721 ctattgatac accaaaacga atgacactat tcataatgct tccctcacat taatttttta
    21781 catagctatg atggagcaat aatctacttt gtgcaagtaa attttttata gcgaaggaac
    21841 aatgacaatg ctgggctatg aggagtgtta taaactgctg ttgacagaaa taattattta
    21901 atcattcacc acaccacgac gtaaactatt ggctataaca ataatatggt gagtaatact
    21961 attgattatg gtaaataatg aagtataaag ataaccaacc tgaaatagca ttataagtgg
    22021 tattgaaaca tagaggtttt tatcaaaagc aaaatataca gcaagggaca tgtacagccc
    22081 aagaataaat tcaatgattg atacaatatt aatggtatgg aggctatatt tattctttaa
    22141 caaagtcttt acatgctcta ctgcattgat acggtgcaat gatgcaacat tatattttgg
    22201 tgttctgttg aaatcacttt ttttattgaa cagggcttca atgacagctt tactgttgtt
    22261 gatggacaag ccgataccaa cagcaagtac cattggcaga tagaccaatg gtttaaactt
    22321 tgaatcaaag ctatttttat tatgataaag aatggataat tcagtgtaaa aataaaatat
    22381 tacaactgat agcgttgcta atataaaaat aggcaaatca agatatgcaa attttttaaa
    22441 tccaccataa tatcgcaccc acagtgcaac aggcatcaga agggtaagaa ataccatgag
    22501 caagtaagca aagtttgcac ctaaatgcat aaatgattca atcttaattt tcaatggaag
    22561 ttgagcattg aggatagttt tataaagctt tttgaagact tggatagagc cttttgccca
    22621 tctgaactgt tgtgctttaa aagctgaaat ttcgataggc aattcggcag gtaccgtaac
    22681 atcaggaaga tacaaaaatt tccagccact aatttgcgaa cggtagctca agtctaaatc
    22741 ttcagtgatt gtatcatgct gccagcctcc accatcaata atagcctgtc ttcgccatac
    22801 accggcagta ccgttaaagt taaaaaatct tccggaacga ttcctggcag tatgctcaat
    22861 catgaaatga ccatctaata aaatactctg gcactcagtt aatagtgaat aatggcggtt
    22921 aatatggtcc catctggttt gaaccatgcc gatagttggg tcagtaaaat aatgtatggt
    22981 tttttgtaaa aagtcaggat tgggcataaa atcggcatca aatacagcaa tgaactcgct
    23041 gtcagtatat ttaagcccat tttccagcgc accggcttta aaacccgttc tatctgtccg
    23101 gtggatatag ataatagtat agccttcttt acgcttttgt ttgcagatct tttcagctaa
    23161 cgctgatgtt tcatcagtag aatcatccag aacctgaatc tctaatttct tcctggggta
    23221 ctctaactgt gttaccgcgt tgataaggcg ctccaccaca tattgctcat tgtagatagg
    23281 caattgaacg gtaacctttg gtaaattctt aaaaacagct tttggtttaa ctttgtttct
    23341 actatgttta atgaaaagat acatcgtata atagcggtga gctccaaata ttgagagcaa
    23401 taataatatc agaccataaa aaataagtat aaaaagcttc atagggggta cttccttttt
    23461 ttgtaagaag gataaataca ataatgaaat agtaaaaacg gagtatagcg cataaaaaat
    23521 acttttcaat aatttatgag taccatataa taaatcatta cgttgtatgt caatactatt
    23581 tatcaggagg tatacaatga ctcaggctac tattcaggca ttacagggtt tgcgggattt
    23641 atcaatgatt aagtggcatg taataccgct actggctatt gttttctata tctatacacg
    23701 tgagatgaaa gaggctcgaa caagcggcaa ctggaatgcc atttttgcag gacttacgat
    23761 ttttggtgtg gattttttca atgaaacatg gaatggatgg gttatgcacc ttacacagcg
    23821 ttcagcagta tggacaacac caggggatac tgcactgcgc accatggttg gctggaatat
    23881 tgaaataatg tttatgttct ccattctggg gattattttt tatcacacgt taaccggcaa
    23941 aaacataaag attttaggta ttcccgaaca atggttctgg gctattgggt attcagcttt
    24001 ttgtgtgttt gttgaatgtt tactcaatgt tggtggacac cttgtctggg aatacgaata
    24061 ctggcggttg tcattcaagg gagtgtggct catctttttg tttggatatt ttcatttctt
    24121 cttctttgct aatattgtga ttggactatc cactatcaaa aagaagatta ttgctgttgg
    24181 catcatctat gctgttccaa taatcatgaa cataatagca cttggtttcc tgggatggat
    24241 gtattaaaca gtaactatcg cccacattaa aattgtggtt tataaatttc ttcaggaaat
    24301 ttttttaata tcgttgcagg tagcgcattg cttttggcaa tctgagcata taccatgcca
    24361 ctatgtttga tggtacgttt ctgggtttta aaatcaacat gcaccaatcc aaagcgcggt
    24421 tcgtacccat gctcccattc aaagttatca atgatcgacc agtagaaata ccctcggatg
    24481 tcaacacctt cggtgattgc tttatgaagc atcttgaggt gatcaataag cacacgctgt
    24541 cgccagctat catcattggt gcctatgcca ttttcggtaa tatacatggg aatatgcgag
    24601 aaagcattat gcattagctt tatagccaga taaaatcctt cggggtacgg ttcccatcca
    24661 aggctgacac ataagccttt tttgccacat acggggttgt cagtagcagg tacggccaga
    24721 tcctttaatc gcaatgatag cctgatgaag ttatagaaat taacgcctat aaaatcattg
    24781 gtattgatta gctccctgtg tgatgaaaaa atttgcaagg gcaattttcc cttttttaag
    24841 gagcggataa tagctccatt aaaacaataa tcagcaaacg ctgtaaatag cttatccatg
    24901 atactgttag gattgtacgg ttttgtgatc tgaacattgt gcactatccc aacctttgca
    24961 tcagggtcaa cagatttaat ggtatggtaa gcaatggcat gcatcagcat tgttgtaccc
    25021 agcgctttaa tggctttgag catatttttt tgtccaggag cagatgtacc ggtaatatag
    25081 ccaagcatga caatacttgg ctcgttaatg gtattgtaga attttacctt ccctttcaat
    25141 gttttagcaa caacatcgca aaactgttta aaatgctcaa ggtgttcttt atctctgttt
    25201 aataagccgc cttcgttgca ccaccagata ggaagtgtaa aatgaaacaa tgtcacaaaa
    25261 ggcgttattt tacgcttcag caactcgttt atcatcgtca cataatggtc aagtgccttt
    25321 ttatcaagcg tcccgggctg ggggaaaatc cgcgaccatt ctattgacat gcgatatgca
    25381 ttgcagttga gcttttttaa aagatcaaaa tcctgtttga agcgattcca gtgatcacag
    25441 gcaacaagcg cgctgtcatt atttttgatt gtaccttctt tttcaaaaag ccaccagttg
    25501 ttgtttgtag tgccaccttc tatctgatga gcagcggttg cggtgcccca caaaaatccc
    25561 tttggaaata caatatcttc tttttgcgcc ataacaccct cgctttatta ttgtactaca
    25621 ctataaaaac atctttttta cattattgag ttgctataaa aaaatcaata aaaaaattat
    25681 tatacagcaa cctatcattg tggttacaca tactctatga acaacagcaa tgtagattac
    25741 catatatgga attatggaac aaatgagtat gattggcgat agttaccggt aaaaaagtga
    25801 ttgtaaaaaa cctatgaaaa gtgattgaat aaattcatta ccatacttat atgctgtgac
    25861 aatagcattt tgagcgcaaa caaagaatca tgccgttgtg gggatatgtc attctgagcg
    25921 tcagcaaaga atctcgtctt ttgtgaaaga gtatattttc tgggacttcg tccctcagaa
    25981 tgacaaaatg tgttgcgttt acctggaatt ataacggtaa aaaatcatag tgttaaggaa
    26041 aattatatgc cgacaacaat aaaaaatttg ggaccttgca ccgttgtttc tccattagca
    26101 gtaaaatatc gccagcgtga agctgctttt attaatgatg ccattagagt tcgttttaat
    26161 gcattcatag gtaaaaaacc tccctctggt ggtgaaacaa ttgaaagctt tgaagctgca
    26221 ggacctcgtg agaagatttt ttttaatcct gaaacaacac gagtcgctat taccagttgc
    26281 ggaggattaa gtccggggat taatgatgtt atccgttcac tggtgatgga atcgtattat
    26341 ggatatggaa taaatgacat cattggcata ccctatggtt ataatggatt aaatcctgaa
    26401 tataattata cccccatccc cttaaatcct gacaatgtaa aatcaatcca tatgcagggc
    26461 ggaagcattc tgggttcatc gcgtggtggc accagggata tggataaact tgttgataca
    26521 ctgcaaaaac taaacgtcag catcctctat acaataggtg gcgatggtac actgcggggc
    26581 tcacacgaca ttgccatgat tgcattacaa agagggttgc ccctggcagc agttggtatc
    26641 ccaaaaacta tcgacaatga cataagcttt attcagcgtt catttggatt tgagacagct
    26701 ttttctattg ctgtagaggc aatctatgca gcgcatacag aagcaatagg agcacccaac
    26761 gggataggac ttgttaaact tatgggcagg cactcaggtt ttattgcggc aaatgcagcg
    26821 ctggcaagca atgatgtcaa ttttgtatta ttgcccgagg ttccctttga actgtatgga
    26881 gcaaatggat tatgcgcgca tcttgaaaat cggttacaaa gaagaggaca tgctgtcata
    26941 tgtgttgctg aaggtgcagg acaggaaata ttgcaaaaag attctgataa gcaatatgat
    27001 gcttcgggta atgttaaatt agctgacatt ggtgtctatc taaaagaaaa gatacaggaa
    27061 tattttaaac aaaaagagat tgaaattaat ttaaaataca ttgatccaag ctacatgata
    27121 cggagtgctc ccgcagttcc caatgattct atctactgca cattattggg gcagaatgct
    27181 gttcatgcag gtatggccgg aaaaacagat ttagtcattg gccaatggag taatatatac
    27241 acccatgtcc cggttgaggt tgcaatttca aaacgaaaac gcatagaccc ccattcgcgc
    27301 ttctatagca gcgtacttga tgcaacagga caaccttttg cgatgataaa taaataataa
    27361 ttgtattgta aaggaggttt gcaatgccaa aagaacataa aaatgcgcca cttatttgtg
    27421 cagttattac cggtatcgca atagcaggag ctggtatagg catagggaaa agcatgccac
    27481 ttattatagc tctcggaatt attccagcta ttgtttatga aatatacaga acacagggta
    27541 tgtttacaac attggcatca tggttagcgc tgatcgttac aatagcagcg ctctatgtta
    27601 tctataaaaa taccatgatt gatattgccc cctatatatc aaaattta
//
LOCUS       DAOSOX010000013        27254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_1766_length_27254_cov_7.830361, whole genome shotgun sequence.
ACCESSION   DAOSOX010000013 DAOSOX010000000
VERSION     DAOSOX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27254
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_27254_cov_7.830361"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLX22_01950"
     CDS             <1..207
                     /locus_tag="PLX22_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18698.1"
                     /translation="VRKRRCDSPTKYRYSSIQSYVQEGADVGVPIDHHEYFLQLGDSF
                     SERVRRFLQYEEAYRKRYSIAQWV"
     gene            428..2299
                     /locus_tag="PLX22_01955"
     CDS             428..2299
                     /locus_tag="PLX22_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT18699.1"
                     /translation="MALNPLVDSRDVRFVLYEMMDVIQLCKLPKYSAFDKDMFEDMIN
                     LAETMAVEQIYPTFAEGDKEGCNYDGATKKVTIPKCYHPALDAFYEAGFGGMDIDDEW
                     GGMGIPQVIAWACNEYFCAANYPLLMYPGLTHGAAELIEAFGTEEQKHMYLANMYSGK
                     WGGTMCLTEPDAGSDVGALKTKAVRQSDGTYKISGQKIFISSGDNDYYENMIHPVLAR
                     IEGDPPGTKGISIFIVPKYRVNADGSLGEFNDVNCAGIEHKMGIKGSATCTLSFGDNN
                     NCIGWLLGEERKGMKIMFKMMNYARMGVGLQAHSTSSAAYMHATTYSKNRVQGAHVTQ
                     MLNPDAKGVTIINHPDVKRMLLWMKSYVEGERAMIYYLYLNYELAQLLEGDAAKEAQG
                     LVEIITPIEKAGCSDMSVLITSEAMQCYGGYGFVADYPVEQMMRNSKITAIYEGTNGI
                     QSMDLAMRKILMNPEQYNYTILKKRIADTINKAKGIVEDKYIALVERGVQELDKVIEM
                     MKQQMAAGQFLHLFANATPLQQAMYMLLIAWMHLWSLTITLPKMKELVGDKKGEERAA
                     LLKDNADAAFYSGKVLSSQYYLGAEFPKYFGKIEALLGGESAVIKASEEIFTGMLNE"
     gene            2528..4411
                     /locus_tag="PLX22_01960"
     CDS             2528..4411
                     /locus_tag="PLX22_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT18700.1"
                     /translation="MATGVMSLNPLVDTRDTRFVLFEVLGVDKLTQYPMFADFDKDTF
                     EDVLNLAETIAVEQVYPANMEGDKIGVQYEPDTKKVILPECYKPALQAMYDAGFIGLA
                     NDPEIGGMGMPQAIGQCCTEFFTAGSVAFSMYPALTIGALNLIRNFGTQEMKDLYIPK
                     MITGQWTGTMCLTEPDAGSDVGNLKTRAIKLDDGTYKIIGQKIFISGGDYDMPENIIH
                     PVLARIEGDPPGTKGISIFIVPKYRVKADGSLEFNDVVCSGIEHKMGIKASATCSLSF
                     GDTGDCIGWLLGKERQGMKIMFQMMNEARLYVGLQGLAVSSAAYMHAITYARNRVQGV
                     HVSQMLNPDAPKVSIIQHPDVKRMLLWMKSYVEGMRLLTYYVGYQEDLAHALDGEAKK
                     EAQAMIEILIPITKAGNTDNAWKITSEAIQVYGGYGFCSDYPVEQFARDSKILSIYEG
                     TNGIQSMDLTMRKILMNPEQYNYSVFKKRVQETIDRAKGVVDDKYVDVVQKGLEKVDV
                     VISYLKDQMAAGKFLHIFASATPLQQAMSMFVLAWMHLWMLSLTQPKMKELIGDKKGD
                     DLNALLADNSEAAYYNGKVLSSQFYLGAEFKKFFGMVDYIIDGEAAVVKASEHIFTGA
                     PLE"
     gene            4659..7013
                     /locus_tag="PLX22_01965"
     CDS             4659..7013
                     /locus_tag="PLX22_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT18701.1"
                     /translation="MDEKRNINFSFLDCIQQPSIIINRNGTIVFANNSFCQNYSFNED
                     KIKNASIEEICSNHEVLFDAMVSAFSIKGKINIKELSLKNNNILIHGICVPFDSEHVI
                     LYSDVSDGELLRKSKMLVQTILDTIPRQIVIVDHNYSIIMANSEWKRFVNEVLEIPGD
                     SLHHDIIELCDKGGCLYDETKSRFSDAIRAILSGRQSLISFESKIYHNENTRWFQFNF
                     FGYTLNGIKFCVIIFTDITDHKVYQEIIQQQRDTFQKYLDISPFMIVLVNPEGKITLV
                     NSKACEILGYTSEELLGKDWFDTCLPHDVAIDVKKTFIELMKGNIKPVEFYENPIVTK
                     SGELKLIAWHNTIIYDANGTILASMSSGEDITQKKQMEIELLKSSTNLKAIVKAFPDI
                     YVIADKDGYIVDYQYGDEKILYATREETLGKTFYDVMPTHIATLYASAVDDAMSTNTI
                     QSIEYTIQIGDESRWREARLVPVIKNRVLAVIRDVTQRKNAELALIESGKRYRNLVQR
                     IPAAIIEMNIQGDIIFINDYFEKITGFTLSDLKGTHWFRSMIHPQEDKRKIRQFIEQI
                     QQGDVSNFEIHIINKEGEDTYLTITTSNIYNKAGKLERIVCIATDVTPIIAMRENLRE
                     MAIKDELTGLYNRRGFMMLAEQQMKINRRSELGMAIFYVDMDNMKLINDTFGHHEGDK
                     ALIDAAAILRLCFRDSDIIGRMGGDEFAILAIDCNRHDIEIMQNRLIENINEFNAKSL
                     REYQLSLSIGSIFIHDSSGNIEDILVRADKTMYRNKLERKKQRH"
     gene            complement(7010..7546)
                     /locus_tag="PLX22_01970"
     CDS             complement(7010..7546)
                     /locus_tag="PLX22_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT18702.1"
                     /translation="MYYDIIMAGFGGQGIQIISQLVAVAAVNKGLEVTYLPSYGVEKR
                     GGRTNVFLVISDREIGSPVTNHPRALLVMDQNSFDTYIPMLRPDGLLVANGSLINTSS
                     YKRDDIVSCCIFCNEEAIKLGNPKLANMIALGAFLKHINFLTIDDIGNIMNEVIPERL
                     SHTIGPNMQALHYGNTIA"
     gene            complement(7946..8698)
                     /locus_tag="PLX22_01975"
     CDS             complement(7946..8698)
                     /locus_tag="PLX22_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT18703.1"
                     /translation="MQAVFTRPKTLKDTPTHFCPGCTHGIAHRIVAEVIDELGIGEKT
                     IAIPGVGCCVFLYNYLDIDVLEAPHGRAPACATGMKRANPGRIIFAYQGDGDMAAIGT
                     SEVIHAANRGENITVLYINNTVYGMTGGQMAPTTLLGQHTTTTPYGRNFRNEGYPIRM
                     AEMLATLEGVAYSTRVALHKPKYVMQAKKAVAKAFTMQINEMGFSMVEMLSTCNTNWR
                     VSPTEAIDKIENEMIPYFPLGVFKERTGKDFL"
     gene            complement(8708..9757)
                     /locus_tag="PLX22_01980"
     CDS             complement(8708..9757)
                     /locus_tag="PLX22_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT18704.1"
                     /translation="MQMRVLTKGNIALAEGAIQAGCRFYFGYPITPQNDIPEYLAKNL
                     PKVGGTFIPAESEIASINMILGASASGARAMTSSSGPGISLMQEGISYLAGSELPAVI
                     VNIMRIGPGLGGIAPTQGDYNQAVHGGGHGDYRNIVLAPASCQDMFDLAIKAFELADK
                     YRNPTMILADAMVGQFQEACRLTPEKPVIIPDKPWALTGAQGRPAQFIKSLFLGPGEM
                     EIHNLDLKKKYDTIQQNEVLYETRHLDDADIMIIAFGTPSRIAKTAIHLLREEGIKAG
                     LLRPVTLFPFPANAINEYSKRIKKVLVVEMNTGQMLRDVQLSCTADTAVYFYGRPGGG
                     IPFPDDIMREVKKIM"
     assembly_gap    9769..9868
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10124..10483
                     /locus_tag="PLX22_01985"
     CDS             10124..10483
                     /locus_tag="PLX22_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18705.1"
                     /translation="MDNKEKIALIRKGNEYFNQGKIKEAIELFVKTGYKDGLHRIGDY
                     YYYDKRLPLIALKFYRLSGSIKKIDEIMERMVFALGKWLGNDTVKESPYRVKLPPLKV
                     SPKLKMLAQEILEKNKE"
     gene            10681..11271
                     /locus_tag="PLX22_01990"
     CDS             10681..11271
                     /locus_tag="PLX22_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT18706.1"
                     /translation="MDTIHLDENLIAILDKITEIKRSMLWEISETYGLTPLQIQILLL
                     IKRCAKYKKINPTDIVKELYVSKATASSAVKTLLKKGMIEKNINSNDNRSFYVSLTKK
                     AGFIITHIEQTAHTMQRYLSQIDKNDKRIAFGVLARMVMAMQDDGAIDYIALCINCEH
                     CKQLSSNTFMCSFTKRTFQYDGINIGCCNFTDKKII"
     gene            11280..11639
                     /locus_tag="PLX22_01995"
     CDS             11280..11639
                     /locus_tag="PLX22_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOT18707.1"
                     /translation="MDANNQNAIKHDIGVLGKFINIYCNGKHPARAKSLVHGHGKVAL
                     YVNPLEVQLCDECTKLLLYAASKRILCPYNPKPSCKDCTTHCYAEPKRSTIREVMRYS
                     GIRMIFRGSISYIKKFL"
     gene            11652..12848
                     /locus_tag="PLX22_02000"
     CDS             11652..12848
                     /locus_tag="PLX22_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOT18708.1"
                     /translation="MSEFLSDMGQKKEQLKKMIKMLHDGADIEQMKILFKKEFGNVMP
                     HQISMLEEELIKEGMPAEEVHRLCQLHIDLFRESIDAGRVDVPLGHPLYILYTEHSRL
                     LEFAQNLVESATKLIDAPTPDVFARLQQLVSFFKESSVHYLREENVIFPVLEKYGVTQ
                     PPKIMWMEHDTIRAIENNIYETADLLAKNVTMELSQKLQMLSIQLSETLVDHFYKENN
                     ILFPTSYKFFNDDDWELVKQEFDAIGYTTFSPVADISKRGGMTTRMEAGSIELETGKL
                     TLEQLTGMLDTMPVDFTFIDKNDVVCYFNNAPDRIFVRSKAVIGRTVQNCHPDKSIHV
                     VNKLLEEMKQGTRDKAEFWITLNGNLIYITYYAVRKNGEYMGCLEVTQNITDIQKITG
                     QKRLLD"
     gene            12948..13271
                     /locus_tag="PLX22_02005"
     CDS             12948..13271
                     /locus_tag="PLX22_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT18709.1"
                     /translation="MIDEALKNSCVLVNFIDYQNSAIVSKTLIQNSGGTITLFAFDKA
                     QSLSEHTAPFDAFVMVLDGKFTITIGGQGHTVAVGEAIIMPANVPHALIAIEKAKMLL
                     VMIKE"
     gene            13274..13702
                     /locus_tag="PLX22_02010"
     CDS             13274..13702
                     /locus_tag="PLX22_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOT18710.1"
                     /translation="MKYKLYSVFGLLFVMVVCAVYGTAKFMQKTSMPQYCGSCHVMIP
                     QYEDWFYIGKHTQITCVDCHLPHNNDANYLLWKGLDGVKDVVMYYTRLYPEIIHSSSH
                     AKKTIQANCIRCHCEMVSRIDNGAMQCWDCHRYFYHSIIH"
     gene            13729..15240
                     /locus_tag="PLX22_02015"
     CDS             13729..15240
                     /locus_tag="PLX22_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOT18711.1"
                     /translation="MEKLTMLIQKIERKHIIAAIGIVIAVSLVMYLIGVLGPTEPKLV
                     LVGAIPDNEYDPAEWGKVYPLEYESWQKTKEPRPTGKSFYKKGWDTDKVVYDKLSEYP
                     FMALLFKGWGFGIAYNEPRGHWYMRIDQDEIDSSRVKAGGVCLNCKSPYMDALVKQYG
                     NSFFSMPWADAIAKIPVRHKNLGASCIDCHDNKTMSLKLNKEIFKRALAKLGKDEFSR
                     QEMRMLVCAQCHVTYNIPKNEAMQSIGLEHPYDGSRWGNISIENIIKLIKKNTHWGEW
                     KQEVTGFKVGFLRHPEFEFFTRQSVHFNAGLACADCHMPYKRVGSFKISDHNLMSPLK
                     DDLFACLKCHPQSNERLVKQIKEIQDRTLSLLLKAGYHEAVVAKLFEKIHTQFKDGLQ
                     NDPLYITAKDYYLEALYRLIFMGAENSIGFHNPEEAGRILGDSIGFSTKAEGYLRQLL
                     AKKNVDVGNSINLELAKYLNNRGQHKLMFQKQQEFNDPFGTVEKTVPRASLGL"
     gene            15447..15761
                     /locus_tag="PLX22_02020"
     CDS             15447..15761
                     /locus_tag="PLX22_02020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT18712.1"
                     /translation="MRLKQSDIECIKQSFIEVFDEGEIYLFGSRTDDTMRGGDIDLYI
                     VPGNTDSLTQKKFKFLVALKKRIGNQKIDVVIKRGSERSIDVVATKEGILICRHYRDM
                     NF"
     gene            15909..>16813
                     /locus_tag="PLX22_02025"
     CDS             15909..>16813
                     /locus_tag="PLX22_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOT18713.1"
                     /translation="MILTLIHNITLLVALSVIWSPILTLRKNYPNTYRILSGLLFGFS
                     AIAAMMTPFHFEEDIIYDGRSVVIALAGLFGGGVASFVASGVAVFYRIYLGGAGVYAG
                     VASIIVSALSGLAMRRIYKNEPSEIPLLGIIIFAVVTHVMVLSCQLLLPWPKGLTVIT
                     TIGVPYILVLSSGLIAIALVFTTVEKRTLALQHLQAGADLLQDVLSVSPSVAFIMNPI
                     DNTISWASPNVEIVLGYTPDEMMHANWFEESIHEDDISQYYSLFDTLTIENFASAECR
                     IYDKSKAIKWVDIHLHAVRYDDGII"
     assembly_gap    16814..16913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16914..18370
                     /locus_tag="PLX22_02030"
     CDS             <16914..18370
                     /locus_tag="PLX22_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18714.1"
                     /translation="GVITACNDNFITIIGSTREALIGLDTLKLPDEKIVKATKRCLQG
                     EQAEYRDWYKSVTANKVTPVRVLSSPLIVEDTVVGGLAIVEDLSQQIDAEKQKEKLEE
                     QLRQAQKLEAIGRLVGGIAHDFNNILSIISGYAALIKFKIDRDNIIYPDIEEIAHAAR
                     RSADIVKQLLTFTRQQKVNPVRLNPNEIIKTSEKMLAAMIGENIKLRLELAEDIYDIW
                     VDSGMFIQILTNLASNSRDAIKDTGTIKIVTQNKFLDDTFCATHPGCKPGDYAMIQCI
                     DDGSGMDKDTLERLFEPFFTTKEVGKGTGLGLSTVYGIMKQFSGHITVYSEYGQGTVV
                     NIFFPKYTEQMPEIPEISAIENNTKTITVLHNKIIVVEDDTAILGMLQRMLLLLDQDI
                     MAFNDPVDALNAVDAYKDFAILITDVIMPEMNGVELYEKLRRIIHNLKVIFISGYSND
                     AFNDIAFNVENSYFLQKPFTFEDLKMALYTISIK"
     gene            18465..19091
                     /locus_tag="PLX22_02035"
     CDS             18465..19091
                     /locus_tag="PLX22_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18715.1"
                     /translation="MKAELRRQHILDCAKQVFAEKGYYEAYVEDVIKVAHIGKGTIYE
                     YFRNKEDLFLSLLIRSLEEWKKAVNISHEEMKQLNPISYFHYRIQRSLMFLNDDRLLT
                     SIILRTGFGFNPHIQKLIGEFEKNLRKHILHDIRLAKHFGFLRDDVDLQLTTTILIGG
                     IIMIAYHDILNVPNLSKAQIDVLTDKYIDTFIKGLFKMQNVNQYINSQ"
     gene            19268..20893
                     /gene="glpA"
                     /locus_tag="PLX22_02040"
     CDS             19268..20893
                     /gene="glpA"
                     /locus_tag="PLX22_02040"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HOT18716.1"
                     /translation="MKTLEAEVIVIGGGATGCGVLRDCTLRGIDAILIEKNDIASGTT
                     GRNHGLLHSGARYAVRDLESATECIKENRILKKIAWHCIEPTGGLFITLPEDDPNYHN
                     RLLEACKFAGIDAKEISVKKALSIEPNCNPNILSAVWVPDGTIDPFRLTSANILDSIE
                     RGSKVCTHTEVERIMLKQNQVIGVQCYDKTAGEYLEIYAPIIVNASGVWGQHICKTAG
                     IELKMFPSKGSMVIIDYRINNVVVNRCRVPSDGDIIVPGDTVSLIGTTSRKIPYDTID
                     ELVVDDDEIDVLLSDGEKLIPNVSRARVLRAYCGVRPLIAVSGEMDGRDISRGVVLID
                     HKERDNVQGLITIAGGKLMTYRLMAEMTTDLIAKKLGNSKRCLTHKIPLPGSEKRVGY
                     TKKIKYFSGIPNSVVGSTHYRHGERVHNILSLKKRNYGLLCECEMVTLGEVEYAIKNL
                     KVNDIVDLRRRTRIGMGPCQGELCAYRAAGLFTEFGDFTPDESTKLLRDFLEERFKGV
                     KPVLWGDGLREFEFTYWIYQGLFGLGLVEDNNT"
     gene            20890..22185
                     /gene="glpB"
                     /locus_tag="PLX22_02045"
     CDS             20890..22185
                     /gene="glpB"
                     /locus_tag="PLX22_02045"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HOT18717.1"
                     /translation="MRYDCIIIGAGVAGLTAGIRCAKEGLHTAIISSGMSALHFSSGS
                     IDLLGYRDNTVVYKPFEAIDQLTGTIAQHPYTKCGKPLIIEALLFFKQECELQGLFLF
                     DNGNLNHFHVTTLGTLKPTYFSQRSVFNEEVYSRFKAMKKIVIINFEGYRDFHPQLAA
                     AQLSKHALFANCDIVAKQIEMPQLQDDGKHPHEFRSVDIARIFEKEDNIEKIADVIRL
                     AADDADFVVMPAVMGITNFNQIHNKLQSRTRLLVYEVPTLPPSILGMRIDNALKSRFA
                     ELGGVYIAGDKVVRADITDDVVNAIYTNSHPENPMEAQCYILSTGSFFSGGLVSEFNN
                     MYEPIFKLKLHHEPERNKWYNQNFFHSDSHGFLQYGVLTDESLHPYDSNNNIIQNLYC
                     SGAIMAHYNPIKEGCGSGVAISTGYKAAGQIVNLLKHMS"
     gene            22198..23385
                     /gene="glpC"
                     /locus_tag="PLX22_02050"
     CDS             22198..23385
                     /gene="glpC"
                     /locus_tag="PLX22_02050"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HOT18718.1"
                     /translation="MENISFDHCIKCTVCTIYCPVARVTHLYPGPKQSGPDTERLRIK
                     NPELLDISLKYCSNCKRCEIACPSDVKIADIIQTAKWRYSKKELRIRDFLLSRTDLMG
                     TFGTLFSVIVNFFTHLGIVKYFLDVIFKIPSGRTFPNYASGTFTRWFKAVRKQQSTYA
                     DKVVYFHGCYVNYNYPQLGKDVVKVLNALGKGVILTKEKCCGVPLIANGYIKKAQSHG
                     RHNIKVLSSALSDEKTKIVSASSSCTLALKHEYPNLLELDNSPILNTIAYITQFILEE
                     FEKGNILSLQPLSYTVAYHSPCHLERMGSAAFTIELLRKIPALTVKILHSECCGISGT
                     YGFKNEYYGISQSVGNELFRIIEEAKPDFVVTDCETCKWQIEMNTKYKVVHPISLLAE
                     AIE"
     gene            complement(23388..23948)
                     /locus_tag="PLX22_02055"
     CDS             complement(23388..23948)
                     /locus_tag="PLX22_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18719.1"
                     /translation="MKKWIAFTMLLILLSVTGTYSEDCNGISPAALRLLAESTIDPCS
                     ICQKKLQRQAYAIVARELFPGKKLTFASTCPLAKTNDCQDNELIMQNSGYPEKVVIKG
                     KEAAMPEVFYKFFSQDKHIAGIAPDDYTSNSIQTMYKTLKINSPVEAQLEIISYNYSD
                     CPSALYLHDRNALIIFCKILSMKQRQ"
     gene            complement(23945..24757)
                     /locus_tag="PLX22_02060"
     CDS             complement(23945..24757)
                     /locus_tag="PLX22_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT18720.1"
                     /translation="MNYRIYIILFFSIFYCSCLNKVIYYPDNRVAIRGNIPFPIEDVH
                     IKVNDEVTLHGWFVFNNDAEFTLIYFHGNAGNITNRVEKINILYKIPLSVFIIDYRGF
                     GDSSGKPSEGGLYADTLASYDFVINNKQLSPDSIIIYGESLGGAVAAYCATKRNTAGV
                     ILDSAFTDIYSMVKHHSACFMYIFFSEKYPTIDYMQSIRYPVLILHSKDDEVAPFAMG
                     QALYNASLSKQKQFVPLHGSHNANFIVDKATYVNSIQSFIQSLKIASKEHGQ"
     gene            complement(24766..26730)
                     /locus_tag="PLX22_02065"
     CDS             complement(24766..26730)
                     /locus_tag="PLX22_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOT18721.1"
                     /translation="MLPYIRHDLKKAIVPFFIFILCCYFLLYTNSIFAQQYQSTTDDQ
                     KTDIISQELFANINVQQLITLANSRKLSQSKNWWALLHYKKNISGTKSLVDDPKFFLA
                     LDGKYNPESELEATIRYLYSYNDKENPVCRFMARYAWLKKELGVTSPYETYCEKLQEI
                     ISFKDDMFVHLIFPTGYINSPASMFGHTLLTVQSQYNPGLLSSAVNYAANTNETFGPS
                     FAFKGIFGLYRGYFSIQPYYSKVQEYSDLESRDIWEYKLNLNQDEVRMMLFHIWELDN
                     IYSDYYFFDENCSYNLLYLLEVTRPDTHLTDNFFFWVIPIDTLRKVSNAGFIENKAFR
                     PSRTTLIQYMVGHLNKQQLNAIKSIAKGNNPLDVISHYSFNDLEKVQVLDCASEYIQY
                     CYADKQYPQKEYQTIFINVLKLRSTIPNTITYSPPVPPPPEDGHYSSRMSIQMGSFEN
                     DTFSELSYRPAYHDLCDDTSGFVSGSQIIFGEIAARYYWDTQKIQFQRFDIIDIESIA
                     PINSLIKPLSWKIITGFERLPFENNNELLVYQLNTGGGYAFSLTQWLQLYGMAEFSGL
                     LNNDFKNNIEIAPGASIGLLLQPFNKMRLRFHVSDYYYIFGESFNYVAIKTIVQYNIN
                     QRFSFRFDVNYWHHEQWFNQFSGSFIYYFW"
     gene            complement(26765..27235)
                     /locus_tag="PLX22_02070"
     CDS             complement(26765..27235)
                     /locus_tag="PLX22_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HOT18722.1"
                     /translation="MKKGLSILVLFILAAATSIAEAGVVRNNAGCGVGTMIFGDKDGL
                     LFEILATTTNGICGNQTFGMTSGTLGCAPMKGIVSNEKINLYVADNMDNLAKDIAKGN
                     GEYLETLALLMNVPESEKQVIFTKLQTNFNKIYTSSDVTSTEVVKNIEVVLQNS"
BASE COUNT         8772 a         4335 c         5628 g         8319 t
ORIGIN      
        1 gtacggaagc gccggtgcga tagtcctaca aaatatcgct acagcagcat acaatcctat
       61 gtacaggagg gtgctgatgt tggtgtaccg atagaccatc atgagtactt tctgcaactg
      121 ggggacagtt tcagtgaacg ggtgagacgt tttttgcagt atgaggaggc gtatcgaaag
      181 cgctactcca ttgcacagtg ggtgtaaaaa aggggtcaga gcattattgc atatcagttt
      241 taatacgata aaatatattt ttcgtatgtt tgaaataaat tatacaaaaa aatatttttc
      301 attattgaca gaaaaatatc atattataac gttaccttgt gttatggttt gtgttttata
      361 aattaaacat acacgttaaa atgtaataat ataacatgcg ttataattta aaatcaggag
      421 gttttatatg gcgttaaatc cactagttga ttcaagggat gtacgctttg ttctctatga
      481 aatgatggat gtcatacagc tttgcaagct tccaaagtat tctgcatttg ataaggacat
      541 gtttgaggac atgataaacc ttgcagaaac tatggctgtt gagcaaatat atcctacatt
      601 tgcggaggga gacaaagaag gttgcaatta tgatggagca actaaaaagg tcactattcc
      661 aaagtgttat catccagcat tagatgcttt ttatgaagct ggttttggtg ggatggatat
      721 tgatgatgag tggggtggta tgggaatacc tcaggttatt gcatgggcat gtaatgaata
      781 tttttgtgca gctaactacc cacttttaat gtacccgggt ttaacacatg gtgcagcaga
      841 acttatagaa gcatttggta cagaagaaca gaaacatatg tatctggcaa atatgtactc
      901 agggaaatgg ggtggaacta tgtgtttaac cgagcctgat gctggtagcg atgttggtgc
      961 tttgaagaca aaagctgttc gccaatctga cggtacatac aaaatttctg gtcaaaaaat
     1021 atttatatct tcaggtgaca atgactatta tgaaaatatg atacatcctg tactggcacg
     1081 aattgaaggg gatccacccg gaactaaagg aatttctatc tttattgttc ccaaatacag
     1141 ggtaaatgct gacggctcat taggtgagtt taatgatgtg aactgtgcgg gtatagagca
     1201 caagatgggc attaaaggtt cagctacatg tacattgagc tttggcgata ataacaactg
     1261 tattggctgg ttattgggtg aagaaagaaa aggtatgaag atcatgttca agatgatgaa
     1321 ctatgctcgt atgggcgttg gtcttcaggc tcattccact tcatcagcgg catatatgca
     1381 tgctacaacc tattccaaaa atagagtaca gggtgcccat gtaacccaga tgctgaatcc
     1441 tgatgcaaag ggtgtaacta ttatcaatca ccctgatgtt aaaagaatgt tgctgtggat
     1501 gaagtcatat gtggaagggg aacgtgcaat gatttattac ctgtatctga attatgaact
     1561 tgcacaatta ttagaaggag atgcagcgaa ggaagcgcag ggattggttg aaattattac
     1621 tccaattgaa aaagctggat gctcggatat gtcagtactt atcacatcag aggctatgca
     1681 gtgttatggt ggatatggtt ttgttgctga ttatcctgtt gaacagatga tgcgaaattc
     1741 aaaaattact gctatttatg agggtactaa tggcatacag tccatggacc ttgccatgcg
     1801 taagatattg atgaatcctg aacagtataa ctatacaata ttgaagaaac gtattgctga
     1861 tactattaat aaagccaaag gtattgttga agataaatat attgcattag ttgagcgtgg
     1921 tgttcaggaa ttagataaag tgatagaaat gatgaagcag cagatggctg ctggtcaatt
     1981 cctccatttg tttgctaatg caacaccatt acagcaggcg atgtatatgt tactaatagc
     2041 atggatgcac ctgtggagtt taacaatcac attgccaaaa atgaaagagc tggttggtga
     2101 taaaaaaggt gaagaaagag cagctttatt gaaagataat gcagatgcag cattctatag
     2161 tgggaaagta ttatcatctc aatattattt gggtgcagaa ttccctaaat attttgggaa
     2221 aattgaagcc ttgcttggtg gtgaatctgc tgttattaag gcaagtgaag aaatatttac
     2281 agggatgctt aacgaataag agatgcttta aaacaaattt taaaaaaaaa gacaggtaat
     2341 caatacctgt ctttttttta taatatgttt aaacatgcca tacaaaaata aacagtaaat
     2401 aacataaatt atatgttaac aaataattga caaaataaat aaaaaaaata tcgtgaatac
     2461 agctattatg gtatttaaca taataccatc atacatatat taaaattgta atacaggagg
     2521 tcttattatg gcaactggag taatgtcgct gaatcctttg gtagatacaa gggatacgcg
     2581 atttgtttta tttgaagtat taggtgttga taaacttaca caatatccta tgtttgctga
     2641 ttttgataaa gatacatttg aagatgtact gaatctggca gagactattg cagttgagca
     2701 ggtatatcct gcaaatatgg aaggggataa aattggtgtt caatatgaac ctgataccaa
     2761 gaaggttatt ttacctgaat gttataaacc agcattgcag gctatgtatg atgcaggatt
     2821 tattgggctt gccaatgacc ctgaaattgg gggtatggga atgcctcagg caattgggca
     2881 atgctgcaca gaatttttta ctgcaggtag tgttgcattt tcaatgtatc cggctttaac
     2941 aattggcgcg ttgaacctta ttagaaattt tggtacacag gaaatgaaag atctctatat
     3001 tcctaaaatg ataacaggtc agtggaccgg taccatgtgt ttaaccgaac ctgatgcagg
     3061 tagtgatgtg ggtaatctta aaacaagagc aattaaactg gatgatggaa catataaaat
     3121 tattgggcag aagatattta taagtggtgg tgattatgac atgccagaga atattattca
     3181 tcccgtattg gcacgaattg aaggagatcc accaggaaca aagggaatct caatttttat
     3241 agtaccaaaa tacagggtga aagctgatgg ttcattggaa ttcaatgatg ttgtttgtag
     3301 tggcattgaa cataagatgg gcattaaagc ttcagctaca tgttcactta gttttggaga
     3361 tactggcgat tgtattggat ggttgttggg caaagaacgt cagggtatga agatcatgtt
     3421 ccagatgatg aatgaagctc gtttatatgt tggattacag ggattggctg tttcaagtgc
     3481 tgcttatatg catgctatta cctatgcacg taatcgagtg cagggtgttc atgtatccca
     3541 gatgctcaat cctgatgcac caaaagttag tattatacag catcctgatg ttaagcggat
     3601 gttgttatgg atgaaatcat atgttgaagg tatgagactt cttacatatt atgttggtta
     3661 tcaggaagat cttgcacatg ctcttgatgg tgaggcaaag aaagaagcgc aggcgatgat
     3721 tgaaatttta attccaatca ccaaggctgg taatactgat aatgcatgga agataacatc
     3781 agaagctatt caggtttatg gcgggtatgg attttgttct gattatcctg ttgaacaatt
     3841 tgcaagagat tctaaaatac tttctattta tgaaggtact aatggtatac agtctatgga
     3901 ccttaccatg cgaaaaatat tgatgaatcc agaacagtat aattatagtg ttttcaagaa
     3961 acgagttcag gaaactatcg atagggctaa aggcgttgtt gatgacaaat atgttgatgt
     4021 agtacaaaaa ggattagaaa aagttgatgt agtaataagt taccttaaag atcagatggc
     4081 tgcaggaaaa tttttacaca tttttgccag tgcaactcca ttacagcagg ctatgtcaat
     4141 gtttgtattg gcatggatgc atttatggat gctaagcctg acacagccaa agatgaaaga
     4201 gctaattggc gataagaagg gagatgatct taatgcattg cttgcggaca atagtgaagc
     4261 tgcatattac aacgggaagg tattatcatc gcaattttat cttggggctg agtttaagaa
     4321 attctttggc atggttgact atatcataga tggtgaggca gcagttgtta aggctagtga
     4381 acatattttt accggtgcac cattagaata atgaattttt atagtttaca aatgtttcat
     4441 aaagcgcatc acgtaatgat gcgctttttt tattaaattt attgaatggg attgtatcga
     4501 aatcttaata aattatagtc tataccgtaa aaaagctgta taataataga tgctattttt
     4561 aaaataaaaa atagcatcta tatttgacaa attgtaagtt ttatgatatg cgttaaatat
     4621 ttttatagta acaactaaaa tagtacagca taatttttat ggatgaaaaa agaaatatta
     4681 acttttcatt tttagattgt atacagcaac cttctattat tattaataga aatggtacaa
     4741 ttgtttttgc caataatagc ttttgtcaaa attattcatt taatgaagat aaaatcaaaa
     4801 atgcttcaat agaagaaata tgttctaatc atgaagtact gtttgatgca atggtcagtg
     4861 cattttctat caaggggaaa attaatatta aggaactatc gcttaaaaat aataatatac
     4921 tgattcatgg catatgcgtg ccatttgatt ctgagcatgt catcctgtat agtgatgttt
     4981 ctgatggtga attgttaaga aaaagtaaaa tgttggtgca aactatcctt gatacaattc
     5041 caaggcagat agttatcgtt gatcataatt atagcattat aatggcaaac agcgaatgga
     5101 agcgttttgt taatgaagta ctggaaatac cgggagatag tttacaccat gatatcattg
     5161 aattatgtga taaggggggt tgcttatatg atgaaactaa atcacgtttt tctgatgcta
     5221 ttagagcaat actatcgggc agacaatcat tgatttcttt tgaaagtaaa atttatcaca
     5281 acgaaaatac acgctggttt caattcaatt tttttgggta tacattgaat gggattaaat
     5341 tttgtgttat catttttaca gatattactg accataaggt atatcaggaa attatacagc
     5401 aacagcgtga tacattccag aaatatctgg acatttcacc atttatgatt gtgctggtta
     5461 atcctgaagg gaagattaca ctggtaaata gcaaggcatg tgaaatatta ggttatactt
     5521 ctgaagaatt attgggtaaa gactggtttg atacatgcct tccacacgat gtggctattg
     5581 atgtgaaaaa aacttttatt gaactaatga aagggaatat aaaacctgtc gaattttatg
     5641 aaaaccctat agttactaaa agtggggaat taaaactcat tgcatggcat aatacaatca
     5701 tatacgatgc aaatggtact atactcgctt caatgagctc aggtgaagat attacacaaa
     5761 aaaaacagat ggagattgag ttattaaaga gtagcacaaa cttgaaagca atagtgaaag
     5821 ccttccctga tatttatgta atagctgata aagatggcta tattgttgat taccagtatg
     5881 gtgatgagaa aatactatat gctacgagag aagaaacgct tgggaaaacc ttttatgatg
     5941 tgatgccaac gcatatcgca acactttatg caagtgctgt agatgatgcc atgtctacaa
     6001 ataccataca aagcattgaa tatacaatac aaattggtga tgaatcacgg tggcgcgagg
     6061 caaggcttgt gccggttata aaaaaccgtg tgctggctgt tataagggat gttacacagc
     6121 gtaaaaatgc agaacttgca ttaattgagt cgggaaagcg atatcgaaat ttagtacaga
     6181 gaatacccgc tgctattatt gaaatgaata ttcagggcga tattattttt attaatgatt
     6241 actttgaaaa aattacagga tttacactat cagatttaaa aggaacacat tggtttagaa
     6301 gtatgattca cccgcaggag gataaaagaa aaatacggca atttattgag caaatacaac
     6361 agggtgatgt aagtaacttt gagatacaca taataaataa agagggggaa gacacctatc
     6421 ttaccattac tacctccaat atttataata aagctggaaa attggaaagg atagtgtgca
     6481 ttgcaactga tgtaactcca attattgcca tgcgtgaaaa tttacgggag atggcaatta
     6541 aggatgaatt aacgggattg tataacaggc gaggctttat gatgttagcc gagcagcaga
     6601 tgaaaataaa tagacgcagt gagctgggga tggcaatttt ttatgttgat atggataata
     6661 tgaagctcat caatgatacg tttggacatc atgaaggcga taaagcgtta attgatgctg
     6721 cagctatttt acgactgtgt ttcagggatt ctgatattat tggcaggatg ggtggtgatg
     6781 agtttgcaat actggcaatt gattgtaata gacatgatat tgaaattatg cagaaccgac
     6841 ttatagaaaa tattaatgaa tttaatgcaa aaagtttacg ggaatatcag ctatcattga
     6901 gtataggaag tatattcata catgacagtt ccggtaatat agaagatatt ctggtaagag
     6961 ctgataaaac aatgtataga aataagcttg agcgaaagaa acagcgtcat taagctatag
     7021 tattaccata atgtagtgcc tgcatgttgg ggcctatggt atgcgataat ctttccggta
     7081 taacttcatt cattatgttc ccaatgtcat caatagttaa aaaatttata tgttttaaaa
     7141 aggcgcccag tgcaatcata ttggcaagtt tgggatttcc taattttatt gcttcttcat
     7201 tgcaaaaaat acagcatgat acaatatcat cccgtttgta acttgatgta ttgataaggc
     7261 ttccatttgc aacaagtaat ccatcagggc gtaacatggg aatataggta tcaaaagaat
     7321 tctggtccat gacaagaagt gcgcgcgggt ggttggtaac aggtgagcca atctctctgt
     7381 ctgagataac taaaaaaaca ttggttctgc cgccacgttt ttccacacca tacgatggta
     7441 gataggttac ttccaatcct ttattgacgg ctgcaacggc aacaagctgg cttataatct
     7501 gtattccctg tccgccaaat cctgccatta ttatgtcata gtacattgtg aatacctctg
     7561 aaaattgaat tattaaccac actcacggac aatctccttt gtaaggatag gaggttttgt
     7621 cctgtaaaaa taggtactat acgcttctgt cattctggag ctttttcgga atcaaatata
     7681 taacttttga tcctgaatcg tggttcataa tgatatgtac tccattgtca ttctgcaggc
     7741 gcagtcacca ggaatatctt cttgcgtaag atgacgagat ttttcacctt gttcagaatg
     7801 acgttctgca acgagattct tcgtctcaga ataacggcaa tgatgcatgt tcatctcatt
     7861 gtcggcgata aaaaacaaac agtgtgaact tcatccaaaa aaatgcctta tggtgcaaca
     7921 gtattctata cagtaactat cgccactata aaaagtcttt gcctgtcctt tccttaaaaa
     7981 caccaagcgg gaaatagggt atcatttcat tttctatttt gtcaatagct tcagttggag
     8041 aaacccgcca gttagtatta catgttgaaa gcatctccac cattgaaaac cccatctcgt
     8101 ttatttgcat ggtgaatgct tttgccacag ctttttttgc ctgcatgaca tattttggtt
     8161 tatggagggc aacgcgagtg gaataggcaa caccttccaa tgtagccagc atctctgcca
     8221 tacgaatagg atacccttca ttgcgaaagt tacgaccata tggggttgtt gttgtatgct
     8281 gacctaaaag cgtggtgggc gccatctgtc cacctgtcat tccataaacg gtattgttaa
     8341 tgtacaaaac agtaatattt tcaccgcgat ttgcagcatg tattacctct gatgtgccaa
     8401 tagctgccat atcgccgtca ccctgatagg caaaaatgat tcggccggga tttgcccgtt
     8461 tcatgcccgt tgcacatgca ggtgctcttc catgcggcgc ttcaagaaca tcaatatcaa
     8521 gatagttata taaaaaaaca caacacccca caccgggaat ggcaatggtc ttttcgccaa
     8581 tacctaattc atcaataacc tctgctacta tgcgatgagc tataccatga gtacaacccg
     8641 gacaaaaatg ggttggcgta tcttttaatg tttttggtcg tgtaaatact gcctgcatgg
     8701 taacctctta cataattttt ttaacttcac gcataatatc atccggaaat ggtatgccac
     8761 caccgggcct tccataaaaa tagacagcgg tatcggcagt acatgatagt tgtacatccc
     8821 tgagcatctg tccggtgttc atttcaacaa ccagcacttt tttaatgcgt tttgaatatt
     8881 cattgatagc attagctgga aatgggaaaa gggttaccgg acgtagtaag ccggctttta
     8941 tgccttcttc gcgcaaaaga tgtatagctg tttttgcaat gcgtgatggt gttccaaatg
     9001 caataatcat aatgtctgca tcatctaaat ggcgcgtttc atacagtact tcattttgct
     9061 gtatggtgtc gtattttttc tttaaatcaa gattgtgtat ctccatctca ccgggaccta
     9121 aaaacaatga tttaatgaat tgagcaggtc ttccctgtgc accggtaaga gcccatggtt
     9181 tatcagggat gataacaggt ttttccggtg taagacggca tgcttcctga aactgtccta
     9241 ccatggcatc cgctaaaatc attgtgggat tacggtattt atcggctaat tcaaatgctt
     9301 ttatggcaag gtcaaacata tcctgacatg aggcgggggc taacactata ttacggtagt
     9361 caccgtgacc gcctccatga actgcctggt tgtagtcacc ctgtgttggt gctataccgc
     9421 caagcccggg accaatacgc ataatgttaa caatcacagc gggaagctct gaccctgcta
     9481 aatatgaaat tccttcctgc ataagcgata taccaggtcc ggaagatgat gtcattgctc
     9541 gggcgcctga tgctgaagca cccagtatca tgttaattga agcaatctcg ctttcagcag
     9601 gtatgaaggt gccgccaacc ttagggagat tttttgcaag gtattcggga atatcatttt
     9661 gaggcgttat gggatagcca aaataaaatc tgcaaccagc ctgtattgct ccttcagcta
     9721 acgctatatt ccctttggtt aatactctca tttgaatacc tcaattgcnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ctaccagtta acgttaactg tcaataaata
     9901 ttataaataa atgcttgcaa aatgatcgat aatagtataa acgcaagcat aaaagctgat
     9961 taatatttaa taattatcaa atctatcgct ataggggata tctgtcaata aaaagtggta
    10021 ggtgtgtaca aaattgtgat gtgaacaaat ttgtaataat taaaaatact ttcatgggcg
    10081 atagttactg ataatgatat ttttatatag taagggatga actatggaca ataaagaaaa
    10141 aattgcttta atccgtaaag gaaatgaata ctttaatcag ggaaagataa aagaagcgat
    10201 tgagctgttt gtaaagacag gatataagga tggattgcac cggataggcg attattacta
    10261 ttatgacaaa cggttgccac ttattgctct taagttctat agattgagcg gcagtataaa
    10321 gaagatcgat gaaataatgg aacgcatggt atttgcgctt ggtaaatggt tggggaacga
    10381 tacagtaaag gaaagtccat accgtgtaaa acttcctccg cttaaggtat ctccaaagct
    10441 aaaaatgctg gcacaggaga tactggaaaa gaataaagaa taaagaataa agaataaaga
    10501 ataatgggca taaatctttt acacataaaa cattataccg gcaagccata ctaactgcta
    10561 cagcaatgaa aaaattataa tctcaatgca ataaaattat tattttatta aaaataattt
    10621 gacgttttct acgtctgtaa tatagttagt actactaact atattacagg acagtacata
    10681 atggatacaa tacatctaga tgaaaactta atagccattc ttgataaaat tactgaaatt
    10741 aaaagatcca tgctctggga aatatctgaa acatatggct taactccatt gcaaattcag
    10801 atattgctat taatcaaacg atgtgcaaaa tataaaaaga ttaatccaac agatattgtc
    10861 aaagaattgt atgtgagcaa agcaacagca agcagtgcag tcaaaacctt attgaaaaag
    10921 ggaatgatag aaaaaaatat caatagcaat gataatcgca gcttttatgt gtcattaaca
    10981 aaaaaagcag gctttattat aacgcacatt gaacaaactg cacatacaat gcaacgctat
    11041 ttatcacaaa ttgataaaaa cgataaaagg atagcatttg gcgtgcttgc ccgtatggta
    11101 atggcaatgc aggatgatgg agctattgat tatatagcgt tatgtattaa ttgcgagcat
    11161 tgcaaacagc tatcaagcaa tacttttatg tgttcattta caaaacgcac atttcagtat
    11221 gacggtatta acattggctg ctgtaacttt actgataaga aaattatata aggaaagcta
    11281 tggatgctaa caaccagaat gcaataaagc atgacattgg tgtgctggga aaattcatta
    11341 atatatactg caatggaaaa caccctgcaa gagcaaaatc gttggttcat ggacatggga
    11401 aagtggcgtt atatgttaat cctcttgaag tgcaattatg tgatgagtgt acaaaattgt
    11461 tgctgtatgc cgcgagcaag cgtatactat gcccatacaa tccaaaacct tcctgtaaag
    11521 attgcaccac acattgttat gcagaaccta aaagaagcac aatacgtgaa gtaatgcgat
    11581 actcaggtat acgcatgata ttcagaggaa gtatttcata tattaaaaaa tttttatgaa
    11641 tggaggatgt aatgagcgaa tttttaagtg atatgggaca aaagaaggaa cagcttaaaa
    11701 agatgataaa aatgcttcat gatggtgcag atattgaaca gatgaagata ctgtttaaaa
    11761 aagaatttgg caatgttatg ccgcatcaga tcagtatgct tgaagaggaa ctgattaagg
    11821 aaggtatgcc agcagaggaa gtacacagat tgtgtcagct tcacatagat ttattcaggg
    11881 aatcaattga tgccggaagg gttgatgtgc cactgggaca ccctttatat attctgtata
    11941 ctgaacatag caggttgctt gaatttgcgc agaatctggt tgaaagtgca acaaaactta
    12001 ttgatgctcc aacgcctgac gtatttgcgc ggttacagca actggtgagt ttttttaagg
    12061 aatcatcagt gcattatctt cgtgaggaga atgttatatt ccctgttctg gaaaaatatg
    12121 gtgtaactca gccacctaag attatgtgga tggaacatga taccatcagg gcaattgaaa
    12181 ataatatata tgaaacggct gatttattgg caaagaatgt taccatggaa ctatcgcaaa
    12241 aattacaaat gctttcaata cagctttctg aaacattagt cgaccacttt tacaaggaaa
    12301 ataatatcct gtttccaaca tcatataaat tctttaatga tgatgactgg gaacttgtga
    12361 aacaggagtt tgatgcaatt ggatatacta cgtttagccc tgttgcagat ataagtaaac
    12421 gtgggggaat gacaacaaga atggaagctg gcagcattga acttgaaacg gggaaactta
    12481 cgcttgagca gttaaccggt atgcttgata cgatgccggt tgattttaca tttattgaca
    12541 aaaacgatgt agtgtgttat tttaacaatg cacctgatcg tatctttgtg cgctcaaaag
    12601 cagttattgg caggactgtg cagaattgcc accctgataa aagtattcac gttgtaaaca
    12661 aactgcttga agagatgaaa cagggcacac gtgacaaagc agaattctgg attacgctca
    12721 atggtaacct tatttatatt acctattatg cagtgcgtaa aaatggtgag tatatgggat
    12781 gtctggaggt aacccagaat attactgata ttcagaagat aacggggcaa aagagattgc
    12841 tggattgatt gtgcgggata ttaatgtgat ataaagctgt gttaaaggga tgctgaaacg
    12901 agtttagcat gacaatggcg cataaatcta tttcaggaga ggtagccatg atagatgaag
    12961 ctttgaaaaa ttcatgtgtt cttgttaatt ttattgacta tcaaaacagt gctattgtaa
    13021 gcaaaaccct tattcaaaat agtggtggta ctattacact ttttgctttt gataaggctc
    13081 agagcctaag tgagcatact gcaccttttg atgcgtttgt tatggtgctg gacggaaagt
    13141 ttacgattac cattgggggg cagggacata cggttgctgt cggggaggct attattatgc
    13201 ctgctaatgt accccatgca cttatagcta ttgaaaaggc aaagatgctg ctggttatga
    13261 ttaaggaata gccatgaaat ataaactata tagcgtgttt ggtttattgt ttgtaatggt
    13321 agtatgtgct gtttatggta ctgcaaaatt tatgcaaaaa acttcaatgc cacagtattg
    13381 tggaagctgt catgtgatga ttccccaata tgaagattgg ttttatattg gcaaacatac
    13441 acagattaca tgtgtggatt gtcatctgcc ccataacaat gatgccaatt atctgttatg
    13501 gaaagggctg gatggtgtta aggatgttgt gatgtattat acacgattat atccggaaat
    13561 tattcattct tcatcacatg caaagaaaac aattcaggct aattgtattc gctgccactg
    13621 tgagatggta tcaagaattg acaacggtgc catgcaatgc tgggattgcc atcgctattt
    13681 ttatcattct attattcatt agtaactatc gaacataaga ggtaagccat ggaaaaatta
    13741 actatgttga ttcaaaaaat tgagcgaaag cacatcattg ctgcaatagg tattgtgata
    13801 gcggtatcat tggtgatgta tcttattgga gtgcttgggc caaccgaacc aaagcttgtg
    13861 cttgttggag ctattcctga taatgagtat gaccctgctg aatggggcaa ggtttatccg
    13921 ttagaatatg agtcatggca aaagacaaaa gagccaagac caaccggaaa aagtttttat
    13981 aaaaaaggat gggacactga taaagttgtg tatgataagc tttcagaata tccattcatg
    14041 gcattgctct ttaaagggtg gggttttggt atagcgtata atgagccgcg tgggcactgg
    14101 tatatgcgca ttgatcagga tgaaattgat tcttcacggg taaaggctgg aggggtatgt
    14161 ttaaattgta aatcaccgta catggatgct ctggttaagc agtatggcaa ttcatttttt
    14221 agcatgccat gggctgatgc tattgctaaa ataccggtaa ggcataaaaa tcttggagcc
    14281 agttgtattg attgccatga caataaaacg atgtcattga agttaaacaa agaaatattt
    14341 aagcgagcac ttgcaaaatt gggaaaggat gaattttcac ggcaggagat gcggatgctg
    14401 gtttgcgctc agtgtcatgt tacctataat atacctaaaa atgaagcgat gcaatcaatt
    14461 gggctggaac acccttatga cggcagcagg tggggcaata tttcaattga gaatatcatc
    14521 aagcttataa agaagaatac tcattggggc gagtggaaac aggaggtcac cggttttaag
    14581 gttggatttc ttcgtcatcc ggaatttgaa ttttttacac gacaaagcgt acatttcaac
    14641 gccggacttg cctgtgctga ttgccacatg ccgtataaac gggttggatc gtttaaaatt
    14701 tcagatcaca atctgatgag cccgctgaag gatgatctat ttgcctgctt aaagtgtcat
    14761 ccgcagtcaa atgagcgcct tgtaaaacag atcaaagaga ttcaggacag aacactgtct
    14821 ctgcttttga aagcaggata tcatgaagcg gtggtggcaa agctatttga aaaaattcat
    14881 acacagttta aggatggttt gcaaaatgat cctctgtata taactgctaa agattattac
    14941 ctggaagcgc tatacaggct tatctttatg ggtgcggaaa actctattgg atttcataat
    15001 ccggaagaag cgggaaggat actgggcgac tctattggtt tttcaacgaa ggcggaaggg
    15061 tatttgcgac agttactggc aaagaaaaat gttgatgttg gaaatagtat caaccttgaa
    15121 ttagcaaaat accttaacaa cagaggacag cataaactta tgtttcaaaa gcagcaggag
    15181 tttaacgatc catttggaac agtagaaaag acagtacccc gtgcaagttt aggcttatga
    15241 aatgtgttta ttcccataat accatttttg ccattgcaga attattaatc atacgtttat
    15301 gatgtttttt aaaaagagta taagtgtatg ctgttgcgtt gtgatgtatt atacttgact
    15361 atagttgcat gggcttatac gctatgctat gtcaactttg caggatgaga aaattatagt
    15421 gagtacagta tagtgggtgt agtattatgc gattaaagca gtctgatatt gaatgtataa
    15481 aacagagttt catagaagtt tttgatgagg gcgaaatata tctgtttgga agtagaacag
    15541 acgataccat gcgtggggga gatattgatt tatatattgt accaggtaat actgattcat
    15601 taacccagaa gaagtttaaa tttttggttg cattaaaaaa aagaataggc aatcagaaaa
    15661 tagatgtggt tattaaaaga ggtagtgaac gatcaattga tgttgttgca acaaaggagg
    15721 gtatcctgat atgcaggcat tacagggata tgaactttta aaaaagattt ttcataattt
    15781 aaaaaactac tgcttgctcc ataacctgat acaataaaaa tatagttgta ttttttattt
    15841 ttttatagta taattaaata ataaatcatt tctgtttcat gtatgcctac atatagcagg
    15901 tttaacgtat gattttaaca ttgattcaca atataacgct tcttgttgct ctcagtgtga
    15961 tatggagccc aatccttacc ttacgaaaga attatcccaa tacctatcgc atactgtcag
    16021 gattgttgtt tggattttca gcaattgcag ctatgatgac accatttcat tttgaagagg
    16081 acatcatcta tgatggtcgt tcggttgtta ttgcattagc cggattgttt ggcggtggtg
    16141 ttgcctcgtt tgttgcatcg ggtgttgctg ttttttacag gatatatctg ggcggcgcag
    16201 gagtatatgc aggtgttgcc agcattatag tttcggcatt gtcaggttta gcaatgcgca
    16261 ggatatataa aaatgaacca tctgaaatac cattattggg gattatcata tttgcagttg
    16321 tcacgcatgt tatggtacta tcatgtcagt tgctgttacc atggccaaaa ggattgacag
    16381 tcattaccac aataggagta ccctacatcc tggtgctgtc atcaggttta atagcaatag
    16441 ctctggtttt tactactgtg gaaaaacgga cattagccct gcaacatttg caggcaggtg
    16501 ctgatttgct gcaggatgta ttgagtgtaa gcccatcggt ggcattcatt atgaatccga
    16561 ttgataatac cattagctgg gcaagcccca atgtggagat agtattaggg tatacgcctg
    16621 atgaaatgat gcatgccaac tggtttgagg agtcaatcca tgaagatgat atttcacaat
    16681 attattcgtt gtttgatact ttaacaattg agaattttgc ttctgctgaa tgcaggattt
    16741 atgataaatc gaaagcaatt aaatgggtgg atatacacct gcatgctgtt cgctatgatg
    16801 atggcataat tagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatggtgt
    16921 tataacagct tgtaatgata attttattac aattataggt tcaacacgag aggctttgat
    16981 agggcttgat acactaaaac tgcccgatga aaagattgtt aaagcaacca aacgctgttt
    17041 gcagggtgaa caagccgaat acagggactg gtataaatca gtgactgcca ataaagtaac
    17101 gcccgtgcgt gttttgtctt cacctcttat tgtggaagat acggttgttg gcggtctggc
    17161 aattgttgag gatctatcac aacaaataga tgctgaaaaa caaaaggaaa aattggaaga
    17221 gcagttgcgt caggcacaaa aattagaagc gataggcagg ctggttggcg gtatagcgca
    17281 tgattttaat aatattttaa gcattatatc aggatatgct gcattaataa aatttaaaat
    17341 agatagggat aatattattt atcccgatat agaagagata gctcatgcag caaggcgtag
    17401 cgctgatata gtaaagcaac ttttaacatt tacccgtcaa caaaaggtta atcctgtaag
    17461 actaaatcca aatgaaataa ttaaaacaag tgaaaagatg cttgcagcta tgattggaga
    17521 aaatatcaag ttgcgacttg aattggcgga agatatatat gatatatggg ttgactcggg
    17581 gatgtttatt cagattctaa caaatttagc ttcaaactcg cgagatgcta tcaaagatac
    17641 tggcacaata aagatagtta ctcagaataa atttttagat gatacatttt gtgcaactca
    17701 tccgggatgt aaacctggtg attatgcaat gatacaatgt atagatgatg gcagtgggat
    17761 ggacaaggat acccttgaaa gattatttga accatttttt actactaaag aggttggcaa
    17821 ggggacaggg cttggacttt caacagtgta tggaattatg aaacagttta gtgggcatat
    17881 aacagtttat agtgaatatg ggcaggggac tgtggtaaat attttcttcc caaaatatac
    17941 tgaacagatg cctgagattc ctgaaatttc tgccattgaa aataatacaa aaacaattac
    18001 agtattacat aataaaatta tagtcgttga ggatgataca gcaatattag gaatgctgca
    18061 aagaatgctt ttacttttag atcaggatat tatggctttt aatgaccctg ttgatgccct
    18121 taatgcagta gatgcatata aagattttgc tatattaata actgatgtga taatgcctga
    18181 aatgaatgga gttgaattat atgaaaagct aagacggatt atacacaatt taaaagtaat
    18241 tttcatttca ggctattcta atgatgcatt caatgatatt gcttttaatg tggaaaattc
    18301 atattttttg caaaaaccgt ttacctttga ggatttgaaa atggctttgt acactataag
    18361 tattaaatga aatgaaatga taaaaagtta ttgaccaata aaaacaactt ttcattatat
    18421 taaacaatac agaataaata tataaagaaa agataggggt tgcaatgaaa gcagaattgc
    18481 gcaggcagca tattcttgat tgcgctaaac aggtatttgc tgaaaaagga tactacgaag
    18541 cctatgttga agatgttatt aaggtagcac atattggtaa aggtactatt tatgagtatt
    18601 ttcgtaataa ggaagatttg tttttatcgc ttttaataag gtcacttgag gagtggaaaa
    18661 aggcggtaaa cattagtcat gaggaaatga aacaattaaa tccaatatcc tattttcact
    18721 acagaataca acgttcatta atgtttttaa atgatgacag gttgctaaca tcaattatac
    18781 tgaggacagg ttttggtttt aatcctcaca tacaaaaact aataggcgaa tttgaaaaaa
    18841 atttgcggaa gcatatcctg catgatatcc gtttagcaaa acattttggc tttttaaggg
    18901 atgatgttga tttgcaatta acaaccacca tattgattgg gggtatcata atgattgctt
    18961 atcatgatat tttaaatgta cctaatcttt caaaagcaca aatagatgta cttactgata
    19021 aatatattga tacatttatt aaaggattgt ttaaaatgca aaatgttaat caatacatca
    19081 attcacaatg atttttctta tgcattaata ttaaaaaata tttttataat atattaatgg
    19141 aagtataata attaatcagg gacttcggca gcgtaaaatt cagtatggca ttaccttcaa
    19201 taattctttt ttaaggcttt ggtaaagaaa aacaatccat agtaaaatgc tataataagg
    19261 tgattgagtg aaaacattag aagctgaagt gatagttatt ggtggaggtg caaccgggtg
    19321 tggtgtttta cgtgactgta cactgcgtgg cattgatgct atcctcatag aaaaaaatga
    19381 tattgcttcg ggtacaacag ggcgcaatca tggacttttg cattcaggcg cacgatatgc
    19441 tgtcagggat ttagaatcag caacagaatg tatcaaagaa aaccgcattt taaaaaagat
    19501 tgcatggcat tgtattgaac ctaccggtgg tttatttata accctgccag aagatgaccc
    19561 caactaccat aaccgtcttt tagaagcctg taagtttgct ggtatagatg caaaagaaat
    19621 aagcgttaaa aaagcattat caatagaacc caattgcaat ccaaacatac tgtctgctgt
    19681 atgggtacct gacggtacca ttgatccatt tcgtttaacg tcggcaaata ttcttgattc
    19741 cattgaacga gggtcaaaag tttgtactca tactgaagtt gaacgcatca tgctgaagca
    19801 aaatcaggtt attggtgttc agtgttatga taaaacagcc ggtgagtatc tggagatata
    19861 tgctccaatt attgttaatg cctcgggggt atggggacag catatttgca aaacagccgg
    19921 cattgagctt aagatgtttc cttctaaggg ttcaatggtt attattgact atcgcataaa
    19981 taatgttgta gttaatcgat gtcgtgtccc ttctgatggc gatatcattg tacctggcga
    20041 taccgtatcg cttattggaa ccacttcaag aaagattccc tacgacacaa ttgatgagct
    20101 tgttgttgat gatgatgaga ttgatgtttt gctgtccgat ggcgagaagc ttatccccaa
    20161 tgtttccagg gcaagggtgc tgagagccta ttgtggtgtg cggccgctca ttgcagtgtc
    20221 aggcgagatg gatgggaggg atataagccg tggcgtagtg ttaattgatc ataaagagcg
    20281 cgataatgtg caggggctta ttaccatagc tggtggaaag cttatgacat acaggcttat
    20341 ggctgaaatg acaactgatc tgattgcaaa aaaattaggc aatagtaagc gatgccttac
    20401 ccataagata ccgcttcctg gctctgaaaa aagggtaggg tatactaaaa agataaaata
    20461 cttttcgggt attcctaact ctgttgttgg gtctactcat taccgtcatg gtgagcgcgt
    20521 tcacaatata ttgagtttaa aaaaacgcaa ttatggttta ctatgtgaat gtgagatggt
    20581 tacccttggc gaggttgagt atgctataaa aaacctcaag gtgaatgata ttgtagattt
    20641 gaggcgaaga actcgcattg gcatgggtcc ctgtcagggt gagctatgtg cataccgcgc
    20701 cgcaggtctt tttaccgagt ttggagactt tacacctgac gaatcaacaa aacttttacg
    20761 tgattttctt gaggaaaggt ttaagggagt aaaaccggta ctgtggggcg atgggctcag
    20821 agaatttgaa tttacctact ggatttatca gggactattt ggattaggac ttgttgagga
    20881 taataataca tgagatatga ttgtatcatt attggcgctg gtgtagcagg attaactgca
    20941 ggtatcagat gtgcaaaaga aggattgcat acggctatta tctcatcggg gatgagtgca
    21001 ttgcattttt catcagggtc aattgatctt ctgggataca gagacaatac cgtggtgtac
    21061 aaaccatttg aagcaataga ccaattaaca ggaactatcg cccagcatcc gtatacaaaa
    21121 tgtggcaagc ccttgattat tgaggcattg ctttttttta aacaggaatg tgaactgcag
    21181 gggctgttcc tttttgacaa tggaaatctt aatcattttc atgtgacaac gcttggtaca
    21241 ttaaagccaa cttatttttc acagcgcagc gttttcaatg aggaagttta tagtagattt
    21301 aaagccatga aaaaaattgt tattattaat tttgaagggt atagagattt ccaccctcaa
    21361 ctggcagcag cacaactttc aaaacatgca ttgtttgcta attgtgacat tgtagctaaa
    21421 cagatagaaa tgccgcagct tcaggatgac gggaaacatc ctcatgaatt tagatcggta
    21481 gacatagcac gaatttttga gaaagaagat aatattgaaa aaattgcaga cgtaattcga
    21541 ttggctgctg atgatgctga ttttgtggta atgccagccg ttatgggtat aaccaacttt
    21601 aatcaaattc ataacaaatt acaaagcaga acccgattgc tggtgtatga agtaccaaca
    21661 ctgcctccct ccattcttgg tatgcgtatt gacaatgcat tgaaatcgcg ttttgcagag
    21721 ttaggcggcg tttacattgc cggtgataaa gtagtacgtg cagatattac cgatgatgtt
    21781 gtcaatgcta tctacaccaa tagtcatccg gaaaatccta tggaagcaca atgttatatt
    21841 ttatcaacag ggagtttttt tagtggcggg ctggtgagcg aatttaacaa tatgtatgaa
    21901 cccatcttta agctaaaact tcatcatgaa cctgaacgca ataaatggta taatcagaat
    21961 ttcttccact ctgattcgca tggtttttta cagtatggtg tgctaaccga tgaaagttta
    22021 cacccgtatg acagcaacaa caatattatt caaaacctgt attgcagtgg cgctataatg
    22081 gcacattaca atcccattaa agaaggatgc ggaagtggtg ttgctataag tacgggatat
    22141 aaagctgcag ggcaaatcgt taatttatta aaacatatga gttaaccaat gattaaactg
    22201 gaaaatatta gttttgacca ttgcataaaa tgtactgttt gcacaatcta ctgcccggtt
    22261 gcgcgtgtta ctcatctgta tcccggacca aaacagtcgg gccctgatac cgaacggttg
    22321 cgcatcaaaa atcctgaact gctggatatt tcgttaaaat attgttctaa ttgcaaacgt
    22381 tgcgagattg catgcccttc ggatgtaaag attgcagata taattcagac tgctaaatgg
    22441 cgatattcaa agaaagaatt aagaatacgt gattttttac tgagccgtac tgatttaatg
    22501 ggaacttttg gaactttgtt tagcgttatt gtcaattttt ttactcattt aggtatagta
    22561 aaatatttcc ttgatgtcat atttaaaata ccatcagggc gaacattccc caactatgct
    22621 tcgggcacat ttacacgatg gtttaaagct gtaaggaagc agcaaagtac ttatgcagat
    22681 aaagtggtat atttccatgg atgctatgtc aattataact atcctcaatt aggcaaagat
    22741 gtggtaaaag tattgaatgc actgggtaaa ggtgttatcc ttacaaagga aaaatgctgc
    22801 ggagtgccat tgattgctaa cggttatatt aaaaaagcgc aatctcatgg aaggcataat
    22861 atcaaggtgc tttccagtgc cctgtcggat gaaaaaacaa agattgtttc agcatcatca
    22921 agctgtacat tagcgttaaa gcatgagtat cccaatctat tagagcttga taatagccct
    22981 atactcaata ctattgctta tataacacaa tttatacttg aagaatttga aaagggcaat
    23041 attttaagct tacaaccact ttcgtatact gttgcctacc attccccgtg tcaccttgaa
    23101 cgtatgggca gtgcagcctt taccattgag ctattacgta agattcctgc ccttacagta
    23161 aaaatattgc attcagaatg ttgtggcata tcaggcactt atggttttaa aaatgaatat
    23221 tatggaattt cacaatcagt ggggaatgag ttattcagga ttattgaaga agccaaaccg
    23281 gattttgtag ttaccgattg tgaaacatgc aagtggcaga tagagatgaa tacaaaatat
    23341 aaagtagtac accccatctc attattagca gaagcaatag agtaatatta ttgtcgttgt
    23401 ttcatagaaa gtatcttgca aaaaatgatg agtgcattcc tatcatgtaa atataatgca
    23461 gatgggcagt cgctatagtt gtaggaaatt atttccagct gggcttcaac aggtgaattt
    23521 atctttaatg ttttgtacat agtttgtatg gaattgctgg tataatcatc cggggctatt
    23581 cctgctatat gcttgtcttg tgaaaaaaat ttatagaaaa cctctggcat tgctgcttct
    23641 tttcctttta tcactacttt ctccggatat ccactgtttt gcatgattag ctcattatcc
    23701 tgacaatcgt tagtttttgc cagtggacag gtactggcaa atgttagctt ttttccggga
    23761 aataattctc tggctactat cgcatacgcc tgcctttgta attttttttg gcagatagaa
    23821 catggatcaa tagtactttc agcaagaaga cgtaatgctg caggtgatat accattgcaa
    23881 tcctctgaat aggtaccggt aacagatagt aaaatcaata acatagtaaa agcaatccat
    23941 tttttcattg tccatgctcc ttgcttgcga ttttcaatga ttgtataaat gattgtatgc
    24001 tattaacgta ggttgcttta tccactataa aattagcatt atgtgagcca tgcaatggta
    24061 caaattgctt ttgctttgat aagcttgcat tgtacagagc ctgtcccatt gcaaaggggg
    24121 caacctcgtc atctttacta tgcagtatca acaccgggta gcgtattgat tgcatataat
    24181 ctatggtcgg atatttttct gaaaagaaga tatacatgaa gcatgcactg tgatgtttta
    24241 ccattgaata gatgtcagta aaggctgaat caaggataac acctgctgta tttctttttg
    24301 ttgcacaata tgccgcaact gcccctccta acgattcgcc atatataatg atactatctg
    24361 gcgatagttg cttgttattg attacaaaat cataagaagc caatgtgtca gcataaagtc
    24421 caccctcgga gggtttcccg ctactatcgc caaatccccg atagtcaatg ataaaaacag
    24481 aaagcgggat tttgtacaat atgtttatct tttcaactct gttggtaata ttgccggcgt
    24541 tgccatgaaa gtatataaga gtaaactcgg catcattatt gaaaacaaac cagccatgca
    24601 gcgttacttc atcattgacc ttaatgtgga catcttcgat ggggaaagga atattgccac
    24661 gtatagcaac tctgttatcg ggataataaa taaccttatt aaggcaggag caataaaata
    24721 tactaaaaaa aagaataatg tatatgcgat agttcatggc atgtttcacc agaaatagta
    24781 gataaatgat cctgaaaatt gattaaacca ttgttcatga tgccaatagt taacgtcaaa
    24841 tcgaaaacta aaccgctgat taatattgta ctgtactatt gttttaatgg caacataatt
    24901 aaacgattct ccaaatatat aatagtaatc actgacatgg aagcgcaatc gcattttatt
    24961 gaatggttgc aataataaac ctatagaagc acctggagca atttcaatat tgtttttaaa
    25021 atcgttattt aaaagccctg aaaattctgc catcccatat aattgtaacc attgagttaa
    25081 agaaaaagca tagccgccac cagtattaag ctgatacacc agaagctcat tattattctc
    25141 aaagggtaac cgttcaaaac cagttataat tttccagctc aatggtttta taagtgaatt
    25201 tattggtgca attgattcaa tgtcaataat gtcaaatcgc tggaactgta ttttttgcgt
    25261 atcccaataa taccgtgctg ctatctcgcc aaaaatgatc tgagaaccag atacaaatcc
    25321 agatgtgtca tcacataaat catggtatgc aggacgatac gatagttcgg aaaaggtgtc
    25381 attttcaaag cttcccatct gtatgctcat tcggctggag taatgaccat cttcaggagg
    25441 cggaggaact ggcggtgaat acgtaatggt attgggtatg gtgcttcgca atttcaatac
    25501 attaataaat atagtttgat actctttttg agggtattgt ttatctgcgt aacaatactg
    25561 gatatattca gatgcacaat caagcacctg cactttttct aaatcattaa atgaatagtg
    25621 actgattaca tcaagtgggt tattcccttt tgcaatggat ttaattgcat tgagttgttg
    25681 tttattgaga tgaccaacca tatattgtat caaggttgta cgtgaaggcc tgaatgcttt
    25741 gttttcaata aatcccgcat tgcttacctt tcttagcgtg tcaataggta taacccagaa
    25801 aaaaaagtta tcagtaagat gcgtatcagg gcgtgtcact tcaagcaaat agagcaggtt
    25861 gtatgaacaa ttttcatcaa aaaaataata atccgaatat atgttatcaa gctcccatat
    25921 atggaatagc atcattcgca cttcatcctg attcagatta agcttatatt cccatatatc
    25981 tctgctttca aggtcactat attcctgtac ctttgaataa tatggttgta tagaaaagta
    26041 tcccctgtat aatccaaaga tccctttgaa tgcaaatgaa ggtccaaatg tttcatttgt
    26101 atttgctgca taatttactg ctgaggatag caatccgggg ttgtattgcg attgcactgt
    26161 taacaatgta tggccaaaca ttgaagcagg gctattgata taaccggtgg gaaaaataag
    26221 atgaacaaac atatcatctt taaaagagat aatctcctgc aatttttcac agtatgtttc
    26281 atacgggctg gtaactccta attctttttt taaccatgca taccgtgcca taaaacggca
    26341 cacagggttt tctttgtcat tatacgaata taaatagcgt attgtagctt ccagctcact
    26401 ttcgggatta tattttccat caagagctaa aaaaaattta ggatcatcaa caaggctttt
    26461 agtgcctgat atattttttt tataatgaag cagcgcccac cagtttttag attgtgataa
    26521 ttttctggaa tttgccagcg ttataagttg ttgaacgtta atgttagcaa aaagctcttg
    26581 tgatattata tctgtttttt gatcatctgt tgtgctttga tattgttgtg caaaaatgga
    26641 attggtatac aataaaaaat agcagcatag tataaaaatg aaaaaaggca cgatagcttt
    26701 ttttaaatcg tgccttatgt atggaagcaa tgacaatatt cccctaattt ttaaatataa
    26761 ttttttagct attctgcaat actacttcaa tgtttttaac aacctcggtc gaagttacat
    26821 cactagaagt gtaaattttg ttaaaattag tctgcaattt tgtgaaaatt acctgttttt
    26881 cactttcggg aacattcata agaagagcta atgtttccag atactctcca ttgcctttgg
    26941 caatgtcttt tgcaaggttg tccatgttgt cagcaacata aaggttgatt ttttcattgg
    27001 aaacaatccc tttcatggga gcacaaccca gtgtacctga agtcatacca aatgtctgat
    27061 tgccgcaaat tccattagta gtagttgcca aaatttcaaa caacaatccg tccttatcgc
    27121 caaaaatcat tgttccaaca ccacaacctg cattgtttcg tactacacct gcttcagcta
    27181 tgcttgttgc ggctgccaat ataaaaagaa caagtattga taaacctttt ttcataaaaa
    27241 cctccatcaa gagt
//
LOCUS       DAOSOX010000014        24848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2013_length_24848_cov_9.492236, whole genome shotgun sequence.
ACCESSION   DAOSOX010000014 DAOSOX010000000
VERSION     DAOSOX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_24848_cov_9.492236"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..709)
                     /locus_tag="PLX22_02075"
     CDS             complement(281..709)
                     /locus_tag="PLX22_02075"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT18723.1"
                     /translation="MAYQVSVFAENKPGKIERITNVLASQNINIRAITINDSGDYGII
                     KLLLDRPEDGCAALKDEGIAATLKEIVAVKIQDRPGSLHKVAAILKEHSINVDDAYGF
                     TIKPHEESAFVFQVDNPKAAEKVLRDSGYQLLADKELYLL"
     gene            complement(850..2145)
                     /locus_tag="PLX22_02080"
     CDS             complement(850..2145)
                     /locus_tag="PLX22_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT18724.1"
                     /translation="MYWNNNIETISRKDLESLQLTKLNKALQAASVTPFYKKVFEENN
                     INIDAIKTLEHIKDLPFTTKNDLRLSYPDGMLAVPKKKVVRMHASSGTTGKSTVIFYT
                     AKDIRDWADLVARCMVATGASQDDVFQNMMGYGLFTGGLGLHYGAEMLGCMVIPSSSG
                     NTLRQIQLMQDFETTIIHITPSYCLHMVEAIRAEGIDPYALTLKKAYLGAEPYSEATR
                     KKIEEQFGIDAYNSYGLSEMNGPGVAFECTYKNGMHIWEDYYIAEIINPETGEVLQPG
                     EEGELVLTHINREAMPILRYRTRDLTRIIPEPCQCGRTHYRIDRIKGRTDDMLIVSGV
                     NVFPSQIETVLMKFPEIGNNYQIILDRENNLDRMHIKVELYSKLFHGDIKEIDRIKSK
                     LKEALKALITINPRIDLLEPGSLPPSEGKAKRVFDNRTL"
     gene            complement(2291..3142)
                     /locus_tag="PLX22_02085"
     CDS             complement(2291..3142)
                     /locus_tag="PLX22_02085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT18725.1"
                     /translation="MQTQYRFGGPITTSLKKIIVFTSVVYIIQLFGNIVAPGVIEYTF
                     GIHHIGFIHEFKLWQPVTYMFLHGNFFHIFFNMFALWMFGCDLEELFGSKNFMRFYLY
                     SGIGAGIGIAFMNYYIYATNGYNPVTIGASGAIYAILLAYGMIWPNREVLLYFIFPIK
                     MKYLVLLFGGIEFFGTIASAQGLGGNVSHIGHLGGFITGFILLKTRFWLRQKTSFNPF
                     ASISRNYRLAKHKKRIDDRIKAKEIIDTLLDKITHYGYNSLTPEEKQKLEWARRHYYP
                     DNNDIIH"
     gene            complement(3245..4846)
                     /locus_tag="PLX22_02090"
     CDS             complement(3245..4846)
                     /locus_tag="PLX22_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18726.1"
                     /translation="MDNAKPQAIATTVLVVDDEESIRDILSRYLQLKGYTVLTAGNGH
                     TAMDILLSQAVDLVLTDLNMPNVNGRELLKRMAEKFPDIPKIVLSGYGSNEDMLVALK
                     SGAYDFIQKPLTDFGILDKSVERAIERKKLKEERDRYVQQITRLNEIIGLLNKGAAFE
                     EIFDIIAKSLNNVIPFDRLAVAIINDAGQVETRLVSSTKNILININDKFPLSDSSLLK
                     VSETKQYHIINDLEEYYRQNPSSERTKLLLQEGMRSSMTIPLIIANRLKGFLLFASVQ
                     PNTFNNDHIQFAETISGHIAFSIQRGELLHELDMHNKKLEQIVKIRTHQLLKTQKATI
                     FALSSLTELRDPETGYHLDRMRGYCMLLAQIAKYSGVALEISNQYLRDLYDSSILHDI
                     GKVGIPDNILLKPGKLTYEEFEIIKTHTTIGYNALKRASQEMGQDSFLSMAMDVTLYH
                     HEAWDGTGYPYGLSEDEIPLSARIVTIVDVYDALTTIRPYKEAYSHQKSIEIMKSEAH
                     RYDPHLFELFLQNADDFDAIRQKYQ"
     gene            5191..7428
                     /locus_tag="PLX22_02095"
     CDS             5191..7428
                     /locus_tag="PLX22_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT18727.1"
                     /translation="MISIQKFESWAIKPFNAIFNFFSERIAYTFGIAFTTSLGGFIIA
                     AYIALFLIATVRFNVSNTGSDFVMLFSGLTLFVYAPLYYLHFGLLYKAGIPCFTRRLS
                     LINKYVEHNTLHQTMSDEEALQLLDALEHLPRDNMAAALIYPSIVMVVCCLYEAIKGT
                     IANALFLLLGIASAIFIYIFLTYIVAELLSGRMRRKIKWLLVKRGISFDERYILSIRG
                     KFIFINIMVMVAMFELGAMVYYNNYELFSIIPLMFISITGILVFVILFFYLLSIEESL
                     GEIESAAVDLARGGSGLLFLPYLDKELINTSKGFIAAAYEVNDIRKNLEQKVEERTEE
                     LNKALTSLKEKNDLIAKELRIAANIQQGILPQTPSVHNAVKIVAYNRASSDIGGDLYD
                     IIQMQDNCLGVLIADVSGHGIPAALISTMVKISFRESCTNYLLPRRIFADVNKQLVNI
                     IRTQEYATVFFMVIFPTYDVMYANASHRKAVVYKRQSGTIMYWDTGGIFLGALPDEEV
                     GSLFEDNFGILEPGDRVFLYTDGLPESRNWNNEEFSDERVERLVSETVNMSIEEARDY
                     IITEWEKFLGGTPPRDDMTFVFLELDPILYNALQHRERGEQLVHEKKFNLAIEELKKA
                     VIMNPQDINALLLLSRSYIYIHHYDRALPHLIDYIAKKPDNSFAHYLLAIVHYKKNQF
                     EEAYNAALQALTMQSSYIPALYSMAVICKALGRKDEAIQYCKQILKLDAGNIKAQRLL
                     QKLLP"
     gene            complement(7425..9239)
                     /gene="pepF"
                     /locus_tag="PLX22_02100"
     CDS             complement(7425..9239)
                     /gene="pepF"
                     /locus_tag="PLX22_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOT18728.1"
                     /translation="MDNTVKKIPRRDEIPDNDKWDVTPLFASAEEWEKLYAEIENSIH
                     DYATFEGKLHQSFSIFLQAIEFDLSISRSIDRLFTYAHLKSDEDTTNQDAQAMLQKAH
                     SLYQRVSEASSFLVPEIQSIPDTIMSNYLSNHKLTPYVFFIEKILRYKEHTLSKEIEE
                     ILAMAHEIAIAPAEFFKKLDNADLRFGTIEDEQGNTIELSHGNFITFLMSYNRDVRKK
                     AFHAYYEVYANHKFSIAQSLASSVKKDVFYSRVRRFKSCLHQALFPDNIPETVYHNLI
                     DSVGKNLNPLFHYLHYRKEALGLDELHFYDTYVPLIPDVPFTMEYQEAVETCIEALTA
                     LGDDYTSVLHDGLLSGWVDRYENRGKISGAYSSGCYDSPPYILLNYDNTINSLYTLIH
                     ESGHSMHSYFSRKYQPYVYHEYTIFVAEVASTLNEMLLSHYLLNKYQDNPLMQAYILN
                     REIDEIRGTLFRQTMFAEFERIIHTMAENNQPLTYQALTEAYAALLQKYFETTIVIDP
                     ELPMEYLRIPHFYSAFYVYKYATGIAAALSLAQMILSSDDGAKKYMQFLRLGGSMFPL
                     EELKIAGVDMESPDAIANALHYFDNLVNTFIQVHKSLV"
     gene            complement(9415..10812)
                     /gene="nhaC"
                     /locus_tag="PLX22_02105"
     CDS             complement(9415..10812)
                     /gene="nhaC"
                     /locus_tag="PLX22_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOT18729.1"
                     /translation="MRKRLPSFSECIALLTITIALLFAGTVLFKIPIPLLLIVAASIS
                     GVKSYLLGHSWAEIEESIVNGIKGTLPAIMILMTVGILSGSFIASGTIPLLIDYGLKL
                     IHPGWILPAAYIVCSISSLVIGSSWTTAATVGVALVGMANAMHVSPAICAGAVISGAY
                     FGDKLSPLSDTTNLAPGVSDTDLFSHIRAMMFTTIPAWVLAFIGFLIINLYSVPNNNT
                     QSEQIVSLLQTLETSFNLNPVVITPLMLVIIMALCKFPAVVILLSGSTVAILFGWLVQ
                     SQNLLFVLQSSYSGFVIHTSNEIINNLLNRGGILSFLETILIILCATGLGGIWESGGY
                     ITSIHNNILHKIKTSKMLIISTVLTGVISNIIMAEQYLSIVMTGRIYKDAYKRKNLEP
                     YMLSRSLEDGGTVTSALVPWNSCGAFMSSALGVSTLHYLPYTLFNLIMPVVAIVFTAL
                     GFFIYKKNNIKQHNK"
     gene            11081..11872
                     /locus_tag="PLX22_02110"
     CDS             11081..11872
                     /locus_tag="PLX22_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT18730.1"
                     /translation="MHIFDQDIALVETGNNAFKATISENWLINTIPNGGYLIALLSNA
                     MLRQSDKKTTPIITANYIQRCKPGEANVHIECFGQSSQFNRYQASLFQQGNEKIRAWG
                     TFASNNNNQYIERYECKSPDVAAIRDCLQLPETPAFQVLSHVAIRLDPYSWGWTHGEV
                     HDRSIQKGWLAFREQRTYDIPALLLMADVIPPAVFVSLGLVTWVPTIELSINIRNMPH
                     SQWLKFYSRTRFITCGLLETDGEIWDEEERLVLLSRQIAQIRVQR"
     gene            11986..13551
                     /locus_tag="PLX22_02115"
     CDS             11986..13551
                     /locus_tag="PLX22_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOT18731.1"
                     /translation="MKKYCIGSMIICLMSINFLYAETIKPEITKVIVYHDSAMIKKEA
                     KMMLNKAVNEIVITNLPAAMVDESVQVIVKDAALQDVRLETTYLQKGDAKRINTVLEK
                     LNAIKDDITAKQNEIASINSVLEYIKKGSTSPFSIKMTAAEMNSILQLIEQYSNQSYI
                     KIAKLQQALQKLNQEKERLEKELALLNANEASKTLKLTVYNTGNNNATVLLQYITGNA
                     GWDMEYDMRTDTEKSATDITCNALVAQNTGEDWNNVAVELSTAKPFVATQLPQLNPWY
                     LDIYRPVYYKESAKSMAVQDADALSRAPSPEEMPEMQQETIAISFLVKGHKTIPSDGN
                     RYKLAVASSVTGCTLRYTTIPKIFAYIMLEGEFVNPFDFPLQRGSVNVYLDGKYVNSF
                     SLDKIYVPKDTVTIGLGVDESMAVERKLVSKKTEYKGIVNKTKRIEYVYSITLNNGKK
                     RDITVTVHDAIPVSQNEKIEVILINKDTIGATIDSQGKATWNIILGPNQKKELKVHFT
                     VEYPDKVQVAGLE"
     gene            13558..14697
                     /gene="rlmD"
                     /locus_tag="PLX22_02120"
     CDS             13558..14697
                     /gene="rlmD"
                     /locus_tag="PLX22_02120"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT18732.1"
                     /translation="MKKHRKLLYTVAQQYADKHVSPSCQHAGECGGCLLQHITYDAQL
                     LIKKDYCNKVLEGIASIEEVMPSHPLGYRNRMDYVTAFGRMGLRKRGNWRHVVDLEDC
                     LLLQTGSRALFTTIRQKLAGIEDYDYHTHRGYLRYIVLREGFNTGQVMVNFVVARHEN
                     NCKAIIDAIADRVTSMSLVLNDGLTDLSYGRVIEDIKQGYIEEKLNGICYRITPNSFF
                     QANTHVTQMMYTEIAKHVYGRALDIYCGVGSISLFIANNVETVTGVECNPEAVMIAKK
                     NALINAVPNAVFIESDAMNYISTYSDDYDVIVVDPPRTGLEQKVVQKLLDIHAKRIVY
                     MSCNPITFASDAGAIMQEYTLQFCKAYDMFPQTQHVELLAVFDRT"
     gene            14700..15863
                     /locus_tag="PLX22_02125"
     CDS             14700..15863
                     /locus_tag="PLX22_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOT18733.1"
                     /translation="MQLSNSQKAIQRRVYHHVIKRMHTFFTIVQPGFEQIAFNELLHM
                     GIKPLAITKGGIVFRGNLYDSYKANMFSRVATRIIMRLTEFRALHFNEIYRTLRNFPW
                     ELYISSGTSIAIEVSVHKSKLHHTDRIGKEVLNAIEKRMNDFALHINTDQYRVQTIYI
                     RFDHDQCGVSMDTTGDPLYKRGYKQHVGRAPLRETIAAALLYECDITSYDVIIDPMCG
                     SGTFPLEAWCMTNNFFPGINRAFAFMHWPSFRKEACNSLASSLSRGNYGATILAGDRD
                     PSMVTIAKNNCTILGAAITTYSGDFLTEKLTIEGKHILCTMNPPYGKRIADDDTAGIY
                     SRLSYIFENWYPAWSFCIIIPLSLRHVFTVPVTKEIRFNNGGLPVIALIGRER"
     gene            complement(15835..16356)
                     /locus_tag="PLX22_02130"
     CDS             complement(15835..16356)
                     /locus_tag="PLX22_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOT18734.1"
                     /translation="METIRFESLPFVQDILLHPAFNTLKIFAHHGTISCYQHTIAVAQ
                     LAYQWSRLLHLDYIAATRGALLHDFYMYNWRTGGPRLHGLRHPHIALNNAAKHFVISK
                     KEKDIILKHMWPLTPIPPRYGESWVVTLADKMVSLHDYACVLKDKIIAFNIFFSQKLS
                     KLWRAIAPFQSEQ"
     gene            complement(16359..17102)
                     /locus_tag="PLX22_02135"
     CDS             complement(16359..17102)
                     /locus_tag="PLX22_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOT18735.1"
                     /translation="MHDYQLMLFEFIIFSTAGWILEVIYRSLNNKKIVNPGFLTGPYL
                     PIYGIGAVIITTIAYATISMPIIYKCIIYFLALSVLEYVSGVLLYILFGKRYWNYLDE
                     PLNIQGFVCLPFSIIWGLLALAFEYVLLPMFIHFYTTIPETLVNTIAFSGLTIMIIDC
                     LLTHSVVQKLINAKKTPSFLQHTMAIRFQNDALLTLRYFVDNAISYRKKINKMIPPKT
                     LRLVSSIKERYTMFLQSITKMIDNHNKHK"
     gene            complement(17095..17670)
                     /locus_tag="PLX22_02140"
     CDS             complement(17095..17670)
                     /locus_tag="PLX22_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18736.1"
                     /translation="MIKRKQHQILRTAEMMFNRFGVKKTAVDDIAHDARVAKATIYNY
                     FGSKEGLLKALILEKIQQFDQKLQELLTANTQPIEKMKTLIREHIQLFINNPFIKDSS
                     LHDEDFSEELNASEQHMLQNIISEASKAGLLHNANSQQIINTMQLFLKGIEATLKNSI
                     TYSLDSLESSIDFISSSIFKSNPIHGDKTNA"
     gene            18119..18694
                     /gene="purH"
                     /locus_tag="PLX22_02145"
                     /pseudo
     CDS             18119..18694
                     /gene="purH"
                     /locus_tag="PLX22_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
     gene            18784..20766
                     /gene="acs"
                     /locus_tag="PLX22_02150"
     CDS             18784..20766
                     /gene="acs"
                     /locus_tag="PLX22_02150"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOT18737.1"
                     /translation="MAEVTLKEIYPVPEKFRAKAYIKSRAEYEKIWKESLSNPEDFWG
                     KIAEEYVTWFKKWDKVMDCNYGTTSKDLWVRWFVNGKVNVSYNCLDRHLEKRGNQIAL
                     IWEGNEPTEDKKFTYKELHEQVCKFANVLKKNGVKKGDRVTFFLPMIPELAIGILACT
                     RIGAVHSVVFGGFSAEALRDRIQDCKSEVLISCDGTFRGAKAVPQKDTADKAIAECPT
                     VRLHIVVKRVGSKIQVNWNDKMDKWYEEEMANADANCPAEQMDAEDPLFILYTSGSTG
                     KPKGVMHTTGGYLTYVAYTHKMIFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATT
                     IMFEGVPTYPDFGRFWAIVEKYKVNIFYTAPTAIRALAKEGDSWVDKHDISSIQLLGS
                     VGEPLNPEAWNWYYHKIGRGECPIVDTWWQTETGGILITPLPGAIDIKPAMATVPFFG
                     VEPCIVDDNGTELEGVCTGNLCIKRAWPGQMRGVYGDPERFFNTYFTQFPGKYFTGDG
                     AKRDKDGYYQITGRVDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGYPHEIKGQSI
                     YAFVTLNTGVEKSDELKKELVAHVRKEIGPIATPEMIQWADVLPKTRSGKIMRRILKK
                     IAAGEFDKSGMGDTSTLADPTVVDTLINDAKKLQGK"
     gene            complement(20981..21499)
                     /locus_tag="PLX22_02155"
     CDS             complement(20981..21499)
                     /locus_tag="PLX22_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT18738.1"
                     /translation="MNGYVQCYTGNGKGKTTAALGLAVRAAGWSKHSIIIQFLKGKKS
                     GERIALKRFHDLITIETYGSKNFYIPGTDDEYFKELTRKAYDRCLAVCTDSSFNIVIC
                     DEILTALQWKLLSEEDIIHLVEIKHHETELILTGRGLSKTLFPYCDLITEMKEIKHYY
                     NNGVKARRGVEW"
     gene            complement(21502..22764)
                     /locus_tag="PLX22_02160"
     CDS             complement(21502..22764)
                     /locus_tag="PLX22_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT18739.1"
                     /translation="MDTIAIKNFTLKRYIHVLLPLFIGSIIAYLDRVNIAYAALTMNQ
                     DLQFTPEIFGMGAGIFFIGYILFEIPGAIIAEKWNPKGWLMRIMITWGIVSFLMIFIT
                     RPWHFYTLRFLLGSAEASFYPVLYASVIPRWFTLQERPKAIALMLTSLQVSAIIGSPL
                     AGFLLDVSLFGLKGWQLLFVCEAIPALFFGILLYFILSNDPMKVSWLSDKEKEYLLNA
                     LSDESKQKTQHYTIWQALKDKEVLKLALIYFLWITGFWGFNYWMPTVIKELSGWSNIT
                     IGSLFAIPMTVSLIVMVYIGYSSSKTGEKRLHGAIPLAIAALGLGAGVFIQDPVVNML
                     FVCIAAIGIFGPFGVWWSYPTTFLSGAAAAGAVGLINSSGNIGGYVGPYLIGIIKNLT
                     NSFELSFLILAGMVLISSVMMLTLKKKA"
     gene            22914..22987
                     /locus_tag="PLX22_02165"
     tRNA            22914..22987
                     /locus_tag="PLX22_02165"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22948..22950,aa:Val,seq:cac)
     gene            23357..23548
                     /locus_tag="PLX22_02170"
     CDS             23357..23548
                     /locus_tag="PLX22_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18740.1"
                     /translation="MLIATALSVWDSDSQYCLPSITIVLPFSGWCAVPDNNCKKAFVA
                     VLRITISALRFSDEHCCSL"
     gene            23845..>24848
                     /gene="pyk"
                     /locus_tag="PLX22_02175"
     CDS             23845..>24848
                     /gene="pyk"
                     /locus_tag="PLX22_02175"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOT18741.1"
                     /translation="MRRTKIVCTLGPTSEEKEVIVNLVRAGMNVARLNMSHSTHQMHE
                     KSIALIREIANELNTPIGILADLQGPKIRVGTLKEKVILEPGQQFILTTRDVPGNAIA
                     VSVSLKELPESVHKGQVILLDDGLLELVVDEVENTDIYTTVVRGGELKDHKGINLPNA
                     SIKTSSITQKDISDLEFVIKHDVDMVALSFVRTAQDVVQLRSIMEEFGGSIPIISKIE
                     KHEAVTNIDDIIAASYGIMVARGDLGIEIPIEQVPIVQKMIIEKCNQQAKPVITATQM
                     LDSMIRNPIPTRAEATDVANAVFDGTDALMLSGETAFGQYPVDAAATMAKIAQYAEDS
                     "
BASE COUNT         7857 a         4366 c         5023 g         7602 t
ORIGIN      
        1 agttaaaaca ccaagatagt gatcatagtt tttaaaaagc tccttataat caaaaggcgc
       61 attaaaatag ggtggtgagg taaccatgag gtgtatgctt tcatcaggaa tttcttccat
      121 ggacatacaa ttgccaataa ttagcttgtg ttgtgtagac attgttaacc ttttagcaaa
      181 taatgaaatt acttatttaa atattcattg tattgagaat taaaaaaata gcatgccaaa
      241 cattgcatga aaagctaaat ctaccgattg ggaagtatcc ttacagtaaa tataattctt
      301 tatcagcaag caattgatat cctgagtctc gtaatacttt ttctgctgct ttaggattgt
      361 ctacctgaaa cacaaatgct gactcttcat gtggtttgat agtaaatcca taagcatcgt
      421 caacgttaat agaatgttct tttagtattg cagcaacttt atgaagactg cctggcctgt
      481 cctgtatttt cacagcaact atctctttca atgtggctgc aataccttca tcttttaatg
      541 cagcacaccc atcctctgga cgatccagca aaagtttaat aataccgtaa tcaccggaat
      601 cattgatggt aatagccctg atattaatat tctggcttgc aagaacattg gttattcgct
      661 caatttttcc tggtttattt tctgcaaaca cagatacctg atatgccata gtgcacctcc
      721 ataactgcat aattattatc gatagtgtta ataacagctt ttttcatgca tacaacattt
      781 aacaaaaaga ttttacaatg ttttgcactc tcaagtatat taaaaagtaa cgttttgaat
      841 attccatatt tataacgtac ggttgtcaaa aacacgttta gctttccctt cactgggtgg
      901 cagtgagcct ggctctaata aatcaatacg aggattaata gtaataagtg ctttcagtgc
      961 ttctttgagt ttactcttaa tgcgatcaat ctctttaata tcaccgtgaa aaagttttga
     1021 gtatagctca accttaatat gcattcggtc taaattattt tcacggtcca gtataatctg
     1081 atagttattg ccaatttcag ggaacttcat aagaacagtt tctatctgtg aagggaatac
     1141 attcacgcca cttacaatga gcatatcgtc cgtacgccct ttaatacgat caatgcgata
     1201 atgggtgcgc ccacattggc atggttcggg tataatacgg gtgagatcgc gagtgcgata
     1261 tcgtaaaatt ggcatagctt cacggttaat atgggttaat acgagctcgc cttcttcacc
     1321 aggttgtaat acttcacctg tttcaggatt gattatctcg gcaatatagt aatcttccca
     1381 gatatgcatg ccatttttat aggtacactc aaaagcaaca ccaggtccgt tcatttcgct
     1441 taagccatat gaattatatg cgtctatacc aaattgttct tcaatctttt tacgggttgc
     1501 ttcagaatat ggctcagcgc ccagatatgc ttttttcaat gttagcgcgt atggatcaat
     1561 cccttcagca cgtattgctt ctaccatgtg cagacaatag cttggtgtga tgtgtataat
     1621 ggttgtttca aaatcctgca ttaactgaat ctgacgcagc gtatttcctg aggatgacgg
     1681 tattaccata caccctaaca tctcagcacc ataatgtaat cccaatccac cggtaaaaag
     1741 cccatagccc atcatattct ggaaaacatc atcttgtgat gcaccagtgg caaccatgca
     1801 gcgtgcaacc agatcagccc agtcacgaat gtctttagca gtgtaaaaaa taacagtgga
     1861 tttacctgtt gttcccgagg aagcatgcat gcgcacaacc tttttttttg gtactgccag
     1921 catgccatcg ggataactca ggcgaaggtc attctttgtt gtgaatggca agtctttgat
     1981 atgctccagt gttttgattg catctatatt aatgttattt tcttcaaata cctttttata
     2041 aaagggggta acgctggcag cctgcagtgc tttgtttagt tttgtaagct gcaatgattc
     2101 aagatctttt ctgctgatag tttcaatatt gttattccag tacatggcaa cctcttttaa
     2161 aaatgtacac gcatttactg tgctatagaa gtggctctac gatccatttc tacgatggta
     2221 atgctgtgac attgtcactg cttccatagg ttgtcaagaa gatattgatt tacagtaact
     2281 atcgccgcca ttaatgaatt atatcattgt tgtcaggata gtaatgtctg cgtgcccatt
     2341 ctaatttttg tttttcttca ggtgtaagcg aattatagcc ataatgggta attttatcaa
     2401 gaagtgtatc gataatctcc tttgccttaa tgcgatcatc aattcttttt ttatgttttg
     2461 caaggcgata gttcctggaa attgacgcaa atggattaaa tgaagttttt tggcgtaacc
     2521 agaatcttgt ttttaaaaga ataaatcccg taataaaacc acctaaatgg ccaatatggc
     2581 ttacgttacc acccagcccc tgtgctgatg cgatggtgcc aaaaaattct atgccaccaa
     2641 aaagtaaaac taaatatttc atcttgattg gaaaaataaa ataaagaagg acctctcggt
     2701 taggccagat catcccatat gccagaagga ttgcatagat ggcgcctgaa gctcctattg
     2761 ttacaggatt gtatccattg gtagcataaa tataataatt cataaaagca atcccaatcc
     2821 ctgcgccaat accactgtac aggtaaaagc gcataaagtt tttactacca aaaagctctt
     2881 ctaaatcaca gccaaacatc catagagcaa acatattaaa aaatatatga aaaaaatttc
     2941 catgtaaaaa catatacgta acaggttgcc acagcttaaa ttcatgaata aatccaatat
     3001 ggtggatgcc aaatgtgtat tcgattacac cgggcgcaac aatgttgcca aaaagctgta
     3061 tgatatagac aacgctggta aaaactataa ttttcttaag tgatgttgta ataggtccac
     3121 caaagcgata ttgtgtttgc atgattttcc ccgatttaat gatagtacaa gtctttatta
     3181 agaaatatag tttatgctgc aataaatgca aaattttgtt tcaatgatac tactgccgtc
     3241 caatttactg atatttctgt cggattgcat caaagtcatc tgcattttgt aaaaacagtt
     3301 caaaaaggtg tggatcatat cgatgtgctt cacttttcat tatttcaata gatttttgat
     3361 ggctatatgc ttctttatag gggcgtatgg ttgtgagtgc atcataaaca tcgacaatgg
     3421 ttactatgcg tgccgataat gggatttcat cttctgaaag cccataagga tatcccgttc
     3481 catcccatgc ctcatggtga tataacgtaa catccattgc catggataaa aatgagtcct
     3541 gtcccatctc ctgcgaagca cgtttcaatg cattatagcc aatggttgta tgggtcttaa
     3601 ttatttcaaa ttcttcatag gttagtttcc ctggttttaa caatatgtta tccggtattc
     3661 ctaccttgcc aatatcgtgt aaaatggatg aatcgtataa atcgcgaagg tattggttgc
     3721 ttatttccaa tgcaactcca gagtatttgg caatctgagc aaggagcatg cagtagccgc
     3781 gcatacggtc aagatggtag cccgtttcag gatcacgaag ctcggttaaa ctgctgagtg
     3841 caaatattgt agccttctga gttttaagca attgatgtgt gcgtatcttt acaatttgtt
     3901 ccaacttttt gttatgcata tcaagttcat gaagcagttc gccacgctgt atactgaatg
     3961 caatatgtcc gcttatggtt tcagcaaatt gaatatgatc attgttaaag gtattgggtt
     4021 ggacagatgc aaaaagcaga aaacctttta atcggttggc aataatgagt ggtatggtca
     4081 tgctggagcg cataccttcc tgtaaaagaa gttttgttct ttcagatgaa ggattttgcc
     4141 tgtagtattc ttctaaatca ttgatgatgt gatattgctt tgtttcagaa acttttagta
     4201 gcgaagaatc gcttagagga aacttatcat taatatttat taaaatattt tttgttgatg
     4261 atacaagacg tgtttctacc tgcccggcat cattgatgat agccactgcc agacgatcaa
     4321 atggaattac attgttaaga ctttttgcaa tgatatcaaa aatttcttca aatgcagcac
     4381 ccttgtttaa caatcctatt atttcattta accgggtaat ttgttgcaca tagcgatcac
     4441 gttcttcctt aagttttttt ctttcaatag ctctctcaac tgatttatcc agtattccaa
     4501 aatcggttaa cggtttctgg ataaaatcat aggctccgct ttttaaggca acaagcatat
     4561 cctcattgct accatagccg ctaagcacta tcttgggaat atcaggaaat ttttccgcca
     4621 tccttttcag gagttcccgg ccgttcacat tgggcatgtt aaggtctgtt aatacaagat
     4681 caaccgcttg tgatagaaga atgtccatgg cagtatggcc attgccagca gtaagtacag
     4741 tatatccttt taattgaaga tagcgtgaaa ggatgtcccg tattgattcc tcgtcatcaa
     4801 caaccagtac cgtggttgca atagcttgtg gttttgcatt atccatgatt tttaagggta
     4861 actcacattg taatacatgt atttcttata cagtatggtg cttttggagt tacgtcaatc
     4921 gtaaaattat agaaatacct aaaaaaatag gtaccatcaa atatagaagg attcatctat
     4981 aaaatcgatt gttcacctta taaataatgg ggaaatgagt tctgacgctc ttttttttac
     5041 ccgggacgag catgacgatg aacaattttt agatggtgag tattattgta attatcataa
     5101 gatttttttt gattgatttt tttgcataaa tattacactt taaaactaaa tactaaatat
     5161 ctaaaatact gtgcgtttca ggacatcatt atgatttcga tacaaaaatt tgaatcatgg
     5221 gcaataaagc cgtttaatgc tatattcaat ttttttagtg agcgtattgc gtatacgttt
     5281 ggcatagctt ttacaacaag tttaggcggc tttatcattg cggcttatat agctcttttt
     5341 cttattgcaa cagtaaggtt taatgtcagc aatacaggat cagattttgt gatgctcttt
     5401 tcaggtctta cgttatttgt ttatgctccg ttatattatt tacattttgg cctgttgtat
     5461 aaagcaggca tcccatgctt tacaaggagg ctttcactca tcaataaata tgttgaacat
     5521 aatacgttgc atcaaacaat gagtgatgaa gaagccctgc aacttcttga tgcactggaa
     5581 catcttccac gtgataatat ggctgctgca ttgatttatc cttccatagt tatggttgtt
     5641 tgctgtctgt acgaagcaat caaaggaact atcgccaatg cgcttttcct tttattgggt
     5701 attgcatcag caatttttat ctatatattt ttaacgtata tagtagctga acttttgtct
     5761 ggaagaatgc gtcgcaaaat aaaatggtta ctggtgaaac gtggcatatc atttgatgag
     5821 cggtatatct tgagtatcag gggtaaattt attttcatca atattatggt tatggttgcc
     5881 atgtttgaat tgggtgccat ggtgtattat aacaactatg agttgttttc aataataccg
     5941 ctgatgttta tatcaataac tggaatactt gtgtttgtta ttttgttctt ttatttactt
     6001 tctattgaag aatcgctagg cgagattgag tcggcggcgg ttgatttagc ccgcggggga
     6061 agtggtttgc tctttttgcc ctatcttgat aaagagctta tcaataccag taaaggattt
     6121 attgccgctg cctatgaggt taatgacata cgaaaaaatc ttgaacaaaa agttgaagaa
     6181 agaacagaag aattgaataa agcgctaaca agccttaaag aaaaaaatga tttaattgca
     6241 aaagagctca gaatagctgc aaacatacaa cagggtattt tgccgcaaac accttctgtg
     6301 cataatgcag tcaagattgt agcgtataac agggcatcca gcgatattgg tggtgacctt
     6361 tatgatatta tacagatgca ggataattgt ttaggtgtcc ttattgctga tgtttcaggg
     6421 catggtatcc ctgctgcttt gataagtacc atggttaaga ttagcttccg tgaatcgtgc
     6481 actaactatt tgctacccag aagaatcttt gctgatgtca ataagcaact ggtcaatata
     6541 attcgcacac aggagtacgc tacagtattt tttatggtga tatttcctac ctatgatgtc
     6601 atgtatgcaa acgcttcaca tcgtaaagca gtggtatata aacggcaatc aggaactata
     6661 atgtactggg ataccggtgg tatctttctg ggggcattgc cagatgaaga ggttgggagc
     6721 ctttttgagg ataactttgg aatactggag cctggtgaca gggtgtttct atacactgat
     6781 ggtttgcccg aatcgcgaaa ttggaataat gaagagttta gcgatgagcg tgtggaaagg
     6841 cttgtttcag aaacagtgaa catgtcaatt gaggaggcaa gggattatat tattacagag
     6901 tgggaaaaat ttcttggtgg aacacctccc agagatgaca tgacatttgt ctttctggaa
     6961 ttagacccaa ttttatataa tgcattacaa catcgcgaaa gaggggaaca gcttgttcat
     7021 gaaaagaaat ttaatttagc aattgaagaa ctgaaaaaag cagtcattat gaatcctcaa
     7081 gatataaatg ccctattatt gctctcacgt tcgtatattt atatacatca ctatgacagg
     7141 gcacttcctc accttattga ttatattgca aaaaaacctg ataacagttt tgcacactat
     7201 ttgctggcta tagtccatta caaaaaaaat caatttgaag aagcatacaa tgctgcatta
     7261 caggcattaa ccatgcaatc atcatacatt cccgctcttt atagtatggc agttatctgc
     7321 aaagctttag ggaggaagga tgaagcaata caatattgca aacaaatcct gaagctggat
     7381 gcagggaata taaaagcaca gcgactatta caaaaactat taccttaaac aagggattta
     7441 tgtacctgaa taaatgtatt gaccaggttg tcaaaataat gtaatgcatt ggcaatagca
     7501 tcaggggatt ccatatccac ccctgctatc ttcaactctt ctaacggaaa catacttccg
     7561 cccaatcgta aaaattgcat atactttttt gccccgtcat cactgctaag tatcatctga
     7621 gccaatgata atgctgcagc aatacccgtt gcgtacttat atacataaaa tgcagaataa
     7681 aagtgtggaa ttctaaggta ctccatgggt aattccggat caatgacaat agttgtttca
     7741 aaatattttt gtaataatgc tgcataggct tctgttaagg cttgataggt taaaggctga
     7801 ttattttcag ccattgtatg gatgatacgt tcaaattcgg caaacattgt ttgtctgaac
     7861 aatgtcccgc gtatctcgtc tatttcacgg ttaagaatat atgcctgcat cagtgggttg
     7921 tcctgatact tatttaatag atagtggctt aagagcattt cgtttaatgt ggaagctacc
     7981 tcagcaacaa aaatagtata ttcatggtat acatagggct gatattttcg tgaaaaatat
     8041 gaatgcatcg agtgccctga ttcgtgaatt aaggtgtaaa gactattgat ggtgttatca
     8101 taatttaaaa gaatgtaagg tggtgaatca taacagcctg atgaatatgc tccgcttatc
     8161 tttccgcgat tttcatatcg gtcaacccac cctgataaca gtccatcatg taaaacagag
     8221 gtgtagtcat cacccagtgc tgtcaatgct tctatgcatg tttcaactgc ttcctgatat
     8281 tccatggtaa agggaacatc aggaattaag ggaacatagg tatcataaaa gtgcagttca
     8341 tccaacccaa gagcttcttt gcgatagtgt aaataatgaa aaagaggatt gaggtttttt
     8401 cccacactgt caattaagtt atgatacacc gtctctggaa tattatcagg gaacagagcc
     8461 tgatgaagac agcttttaaa tctgcgtacc cgtgagtaaa atacatcttt ttttaccgag
     8521 cttgccagtg attgtgcaat gctgaattta tgattggcat acacctcgta ataggcatgg
     8581 aaagcttttt tgcgtacatc ccgattatag ctcattaaaa atgtgatgaa attgccgtga
     8641 cttaattcaa tggtgttgcc ctgttcatct tcaattgtgc caaaccgcag atccgcatta
     8701 tccagttttt taaaaaattc tgcaggggca atggctattt cgtgtgccat ggctaatatt
     8761 tcttctattt cttttgataa tgtatgttcc ttgtagcgaa gtatcttttc tataaagaat
     8821 acatacggcg taagcttgtg gtttgacaga tagttactca taatagtgtc aggtatggat
     8881 tgaatctcgg gcactaaaaa gcttgatgcc tcactgacac gctgatacaa cgagtgagct
     8941 ttttgcaaca ttgcctgagc atcctgattg gttgtatcct catcgctttt gagatgtgca
     9001 taggtaaaca atctgtcaat ggagcgcgag atggaaagat caaactcaat agcctgtaaa
     9061 aaaattgaga aggactgatg gagcttgcct tcaaaagtag cataatcatg tatagaattt
     9121 tcaatctctg cataaagctt ttcccactct tctgctgaag caaacagtgg tgtcacatcc
     9181 catttatcat tatctggaat ttcatcccgg cgtggtattt tttttaccgt gttgtccatg
     9241 gtagctcctc attacagttt ttaagatttt tattaataat ggtatattca tgtataatcg
     9301 ttattaaaaa tattgtacag tgtgccctgc agcaatgata tgaagtattt gcaagtgatt
     9361 ctttgcctcc atataaaaca attgtgtcat ttaaaacatt atcgtattat tgtatcattt
     9421 attatgctgt tttatattat ttttcttata gataaaaaag cccagagctg taaatactat
     9481 tgcaacaaca ggcataatga gattaaataa tgtatagggt aaataatgca gtgtggaaac
     9541 acctaatgcg gagctcatga aagcgccaca tgaattccat ggtacaagcg cagatgtaac
     9601 ggtgccacca tcctcaagag agcgggaaag catatatggc tcaagatttt tccttttgta
     9661 ggcatcttta tatattcgcc ctgtcataac aattgataaa tactgttcag ccataataat
     9721 atttgaaatg actccagtta aaacagtgga gataataagc attttgcttg ttttgatctt
     9781 atgaagaata ttgttgtgaa tggatgtaat atatccgcca ctttcccata tgccacccag
     9841 gcctgttgcg cacagtatga tgaggattgt ttctaaaaaa ctaagtatac cgccacgatt
     9901 taatagatta ttgatgatct cattgctggt atgaataaca aatccggagt atgatgattg
     9961 taaaacaaaa agtaaatttt gtgattgtac aagccagcca aacaggatgg cgacagtact
    10021 ccctgataac aggattacaa cagctggaaa tttacataat gccattataa tgacaagcat
    10081 aagtggagtt atgacaacag gattcaaatt gaaagaagtt tccaaagttt gtagtaatga
    10141 cacaatttgc tctgattggg tattgttgtt gggaacacta tacaggttta taattaaaaa
    10201 gccaataaaa gcaagaaccc acgcggggat tgtggtaaac atcatagctc tgatatggga
    10261 aaataaatct gtatcggata cacccggtgc taaattggtt gtatcagaaa gaggtgatag
    10321 tttatcgcca aaataagcac ctgatataac agcaccagca catatagctg gtgaaacatg
    10381 cattgcattt gccatcccaa ccagagctac gccaacagtt gctgcggttg tccatgaact
    10441 tccaatgaca agactgctta tactgcaaac aatatatgcg gcgggtaaaa tccatcccgg
    10501 atgtatgagt tttagtccgt aatcaatgag taaaggaata gttcctgatg caataaaact
    10561 acctgataat attcccactg tcatgagtat catgattgca ggcagtgtgc cttttatgcc
    10621 attgacgata ctctcttcaa tctccgccca gctatggccc aatagataac ttttaacacc
    10681 tgaaattgat gctgcaacga tgagtaacag cggtatcggt attttaaata gtactgttcc
    10741 tgcaaaaagc aaagcaatcg taattgttag cagtgcaatg cattcagaaa atgatggtaa
    10801 tctttttctc atactttttt atactatatg tttgccaatt tatagtttgc aataaaaggt
    10861 ggtgtatgat aacgaatatc atagagtata atgatgattt aacgacttga tggcaatcat
    10921 aaaaaaagct tttaataata agtatactgg tattacaatg acaaaatata aatattgagc
    10981 taccatggtg atgatacgtt ataaaaattg tcaaatagaa atagttagaa gctgtatgtt
    11041 atgatactat tttttagaat gttaaaagtg tggacaacat atgcatattt ttgatcagga
    11101 tatcgccctt gttgaaacag gcaacaatgc atttaaagca actatctcag agaattggtt
    11161 aattaatact atccctaatg ggggttatct tattgcattg ttgagcaatg caatgctacg
    11221 acaaagtgat aagaaaacaa ctcctatcat aacagcaaat tatatacaac gttgcaaacc
    11281 tggagaagct aatgtgcata ttgaatgctt tgggcagtct tcacaattta ataggtacca
    11341 ggcaagcttg tttcagcagg gtaatgaaaa aataagagcg tggggaacgt ttgccagtaa
    11401 taacaataac cagtatattg aaagatatga atgcaagtca cctgatgtag ctgctatacg
    11461 ggattgcctt caacttccag agacgccagc atttcaagtg ttatcccatg ttgctatacg
    11521 gcttgatccc tattcatggg ggtggacaca tggagaagtg cacgacaggt caattcaaaa
    11581 aggatggctt gcattccgtg aacaaagaac atatgatatc cctgcacttt tgcttatggc
    11641 agatgtaata cctcctgcag tatttgtaag tttagggctt gttacatggg ttccaaccat
    11701 tgagttgtca attaatatac gtaatatgcc tcactcacag tggttaaagt tttattcgcg
    11761 tacacggttc attacctgtg gcttacttga aaccgatggc gagatatggg atgaagaaga
    11821 gaggcttgtg ctgctctcac ggcagattgc acagatacga gtacaacgat gagattattt
    11881 aagtaactat cgtacaaaga gatgtttaaa aaaattctgc ccgggtggca gacaatattc
    11941 atcatgatag ttaaaacgat attaaattat ataaggagcg aatgtatgaa aaaatattgt
    12001 attgggtcga tgattatctg tttaatgagt attaattttc tttatgccga aacaataaag
    12061 cctgaaataa caaaggtaat tgtataccat gatagtgcta tgattaaaaa agaagcaaag
    12121 atgatgctga ataaagcggt aaatgaaatt gtcatcacaa acttacctgc tgcaatggtt
    12181 gatgaatctg tgcaggttat tgtgaaggat gccgctttgc aggatgtgcg tttagagaca
    12241 acctatttgc aaaagggcga cgcaaaacgt attaatacag ttttggaaaa gctaaacgca
    12301 ataaaggatg atataacagc aaaacagaat gagatagctt caattaattc agtgctggaa
    12361 tatatcaaga aaggcagtac ttcgccattt tccataaaga tgaccgctgc tgaaatgaat
    12421 tctatattac aattgattga gcaatattcc aatcaaagtt atatcaaaat agcaaagtta
    12481 cagcaagcac tgcaaaagct caatcaggaa aaagagcgtc tggaaaaaga actggcactg
    12541 ctcaacgcaa atgaggcaag taaaacgctg aaattaactg tatacaatac agggaacaac
    12601 aatgcaacag tacttttaca atatatcacc ggtaatgctg gctgggatat ggaatatgac
    12661 atgcggacag atacagaaaa atctgctaca gatatcacat gcaatgccct tgttgctcaa
    12721 aataccggtg aagactggaa caatgttgca gtggagctat cgacggcaaa accctttgta
    12781 gcaactcaac ttcctcagct caatccatgg tatctggata tttatcgccc tgtatattat
    12841 aaggagtctg caaaaagcat ggcagtacag gatgcagatg cattgtccag agcaccttct
    12901 cccgaagaaa tgcctgaaat gcaacaggag acgatagcta tttcatttct tgttaaaggg
    12961 cataaaacaa taccttctga tgggaatcgt tataagcttg ctgttgccag ctcggttaca
    13021 ggttgtacat tacgatacac taccattcct aaaatttttg catatatcat gttggagggg
    13081 gaatttgtaa atccatttga ttttcctctc cagaggggga gtgtgaatgt ttatttagat
    13141 ggtaaatatg taaactcgtt tagccttgat aagatatatg ttcctaaaga taccgtaacc
    13201 attggattgg gtgtcgatga aagtatggcg gtagagcgta aacttgtaag caaaaaaact
    13261 gaatataagg gaatagtgaa caaaacaaaa cgtattgagt atgtgtattc aattacactg
    13321 aataatggaa aaaagcgtga tataacggta acagttcatg atgccattcc tgtatcacaa
    13381 aatgaaaaga ttgaagtcat tcttataaac aaagatacta ttggagcaac tatcgatagt
    13441 caagggaaag caacatggaa tattatcctt ggtcctaacc agaaaaaaga gttaaaagtt
    13501 cattttacag ttgaatatcc tgataaagtg caggtggcag gtttggagta gaaaacattg
    13561 aaaaaacatc gtaagctttt atacactgtt gcacagcagt atgcagataa acatgtatca
    13621 ccatcctgtc agcatgctgg tgagtgtggt gggtgtctat tgcaacatat tacctatgat
    13681 gctcagttgt tgataaaaaa agactattgc aataaagtgc ttgaaggtat agcatccatt
    13741 gaagaagtga tgccatcaca tccattggga tatcgcaatc gtatggatta tgtaactgct
    13801 tttggcagga tgggacttag aaaacggggt aactggcgtc atgttgttga tctggaagat
    13861 tgtttgctat tacagactgg tagcagagcg ctctttacaa caatacgaca aaagttggcg
    13921 ggtattgagg attacgatta tcatacccat agggggtacc tgcgatatat tgttttacgt
    13981 gaaggattta atacagggca ggtgatggtt aattttgttg ttgcgcggca tgaaaataat
    14041 tgtaaagcga taattgatgc aatagcggat agggtgactt ccatgtcact ggtattgaat
    14101 gatggattga ctgatctaag ttatggaagg gtgattgagg atataaaaca gggatacata
    14161 gaagaaaagc ttaatgggat ttgctatcgt attaccccca attcgttttt tcaggctaac
    14221 acccatgtta cacagatgat gtatacggag attgcaaagc atgtttatgg cagagctctt
    14281 gatatatact gtggcgtagg aagtattagc ctttttatag caaataatgt tgaaactgtt
    14341 accggggtag agtgtaatcc tgaagcagta atgattgcca aaaaaaatgc attgataaat
    14401 gcagtaccca atgctgtttt tattgaatca gatgccatga attatatcag tacatattct
    14461 gacgactatg atgtaatagt tgttgaccca ccacgaacgg gattggaaca aaaggtagtg
    14521 caaaaacttc tggatatcca tgctaaaagg attgtatata tgtcgtgcaa tcctataacc
    14581 tttgcttccg atgcgggagc aataatgcag gagtatacat tgcaattttg caaggcgtat
    14641 gatatgtttc ctcagactca gcatgtagaa cttctggctg tatttgacag aacataatca
    14701 tgcaactgtc caattcacaa aaagcaatac aacggagggt gtatcatcat gtaattaagc
    14761 ggatgcatac attttttaca attgttcagc ctggctttga acagattgca ttcaatgagc
    14821 tgttacatat gggcatcaaa ccgctggcta ttacaaaagg cgggatagta ttccgcggga
    14881 atctttacga tagctataaa gcaaacatgt ttagccgtgt tgctacacgt attatcatgc
    14941 gtttaacaga attcagggct ctgcacttta atgagattta ccgcacactg agaaattttc
    15001 catgggaatt atatataagc agtgggacat ctattgctat tgaagtatcg gttcataaat
    15061 caaaacttca tcataccgat agaattggta aagaagttct taacgccatt gaaaagagga
    15121 tgaatgattt tgcactgcat ataaatactg accaataccg ggtacaaaca atttatatac
    15181 ggtttgatca tgaccagtgt ggtgtcagta tggatacaac cggtgatcct ttatataaga
    15241 gaggatataa acagcacgtt gggcgggcac cattacgaga aacaatagca gctgcattgc
    15301 tgtatgaatg cgatataacc agctatgatg ttatcattga tcctatgtgc ggtagcggaa
    15361 catttccact cgaagcatgg tgtatgacaa acaatttttt tccagggata aaccgtgcgt
    15421 ttgcctttat gcactggcca tcattcagaa aagaggcatg caattcattg gcatcatcat
    15481 tgagcagggg aaactatggc gcaacaatac ttgccggcga cagggaccca tcaatggtta
    15541 ctatcgccaa aaacaattgt actatacttg gtgctgcaat aactacctat agtggtgatt
    15601 ttcttactga aaaattaacg attgaaggga aacatatctt atgtacaatg aacccgcctt
    15661 atggcaagcg catagctgat gatgacacag caggaatata tagcaggctg tcatatattt
    15721 ttgaaaactg gtatcctgca tggagttttt gtattatcat tcctttatca ctgcggcatg
    15781 tcttcaccgt ccctgtaacg aaagagatac gatttaacaa tggtggattg cctgtcattg
    15841 ctctgattgg aagggagcga tagctctcca caatttgctt agcttctgag aaaaaaatat
    15901 attaaaagct ataatcttat ctttgaggac acatgcataa tcatggagtg acaccatctt
    15961 atcggcaagg gtgacaaccc acgattcacc atagcgaggt ggtatggggg ttagtggcca
    16021 catatgttta aggataatat ctttttcttt ttttgatata acaaaatgtt ttgcagcatt
    16081 gttaagcgct atgtgtggat ggcgtaaacc atgtaagcgc ggaccgccgg tacgccagtt
    16141 gtacatataa aaatcatgca ataatgcacc ccgtgttgca gcgatataat ccagatgcag
    16201 cagacgtgac cattggtacg ctaattgtgc aacagctatg gtatgctgat agcagctgat
    16261 tgtgccgtga tgagcaaaaa tttttaaggt attaaatgca ggatgcaaca gtatatcctg
    16321 cacaaagggt aatgactcaa atcgtatggt ttccatagtt atttatgttt attatgatta
    16381 tcaatcattt ttgttatgct ttgtaggaac atggtgtatc gttctttgat gctgcttacc
    16441 agacgcaatg tttttggtgg tatcatttta tttatttttt tccgatacga gatggcatta
    16501 tcaacaaaat atcgtaatgt cagaagagca tcattctgaa aacgtatagc cattgtatgc
    16561 tgcaagaagg atggagtttt ttttgcatta ataagctttt gaacaacact atgtgtcagc
    16621 aagcaatcta ttatcataat tgttaagccc gaaaaagcta tagtatttac cagtgtttca
    16681 ggtatagtgg tataaaaatg tataaacatc gggagcagta catattcaaa tgccagtgca
    16741 agcaaccccc agataattga aaacggtaaa catacaaatc cctgtatatt cagtggttca
    16801 tctaaataat tccagtaacg cttgccaaac agaatataca gtaacacccc tgatacatat
    16861 tccagcacgc ttagtgctaa aaaataaata atacatttat agataatagg catggaaata
    16921 gtagcatagg cgatagttgt tataataact gcacctatcc cgtaaatggg aagatatggt
    16981 cctgttaaaa aacccggatt tacaattttt ttattattga gagaacgata gataacttct
    17041 aatatccacc ctgctgtaga aaagatgata aactcaaaca gcatcagctg gtaatcatgc
    17101 attggttttg tctccatgta ttggattaga tttaaatatt gaactggaaa taaaatcgat
    17161 acttgactct aaagaatcaa gggaataggt gatactgttt tttaatgttg cttcaatacc
    17221 tttaagaaaa agctgcattg tgttgataat ctgctgacta tttgcattat gtaataaccc
    17281 tgcttttgaa gcttcagaaa tgatattttg taacatatgc tgttcacttg cattgagttc
    17341 ttcagaaaaa tcctcatcgt ggagtgaaga gtctttaata aatggattgt taataaaaag
    17401 ctggatatgc tccctgatca atgttttcat tttttcaatg ggttgagtgt ttgcagtaag
    17461 gagttcctgt aatttttgat cgaattgctg tatcttttcc agtatcaacg ctttgagcaa
    17521 tccctcttta ctgccaaaat agttatagat agttgccttg gcaacccgtg catcatgggc
    17581 aatatcatca acggctgttt tttttacacc aaacctgttg aacatcatct cagccgtgcg
    17641 aagtatctgg tgttgttttc gttttatcat ggtactacca ttttatactt ttatactatt
    17701 ttagtataaa ataatactaa accaggctat cgtcaacaat aaaataaaaa tttttttagc
    17761 atcatgttgt agatatatca aatgagagac aacccgaggt ctatcatgac atcaaggata
    17821 tgctgctgga tgacattatc tggttaatct ggttgttggg agggatttat aattaatgtg
    17881 atgaacaatt attgacagct aaataatgat attttgtgag gtactactaa tcaaaatata
    17941 aagattaaca ttcacaaaaa tataccaagc gttgtattaa aatctgttaa attatacagg
    18001 ttttgttcat ttttaaaaaa tgcttgtatt tttcaaggtg attattcaaa tggtcatatt
    18061 catagtaata caataaaaat tagttttagt ttaatttaaa atgttaaatg gaatttaaat
    18121 ggctgcaatc aaaagagcat tgataagttt gtccgataag tcggggataa taccgtttgc
    18181 taaaaagctc aataatggtg gtgttgcaat tttgtctaca ggaggcactg ccaggcttct
    18241 cagggataat ggtattgatg ctactgaagt ttcagattac accgggtttc cggaaatgat
    18301 ggatgggagg gtaaagacgc tccatccaaa aatacacggt gcgctattgg gattgcgtga
    18361 taatcctgaa catatagaag caatgcagaa gcatggtata atacccattg atatggtggt
    18421 gattaatcta tacccatttc aacagacaat ttccagagga tgtacccttg acgaagcaat
    18481 tgaaaatatt gatattggcg gacctgcaat gcttagatca gctgctaaaa attataaata
    18541 tgtaaccgtt attgttgacc ctgaagatta tgatactgtc ttaagcgaga tggaattgaa
    18601 tgatggatgt gtgtcagaag aaacaaattt taggctggca agcaaagttt ttgagttgac
    18661 atcacagtac gattcaatga ttgctacata tttaaaagaa caaattaaga atgcttaaag
    18721 ggtaaaccgt tgcaagcgga gcccataatg tgcatattac aatataaaca tcggaggtaa
    18781 tagatggcag aagttacatt aaaagagatt tacccggttc cagagaaatt ccgcgcaaaa
    18841 gcgtatataa agagccgggc tgagtatgaa aagatctgga aagagtcatt gtccaatcca
    18901 gaagattttt ggggcaagat tgcagaagaa tacgtgacct ggttcaaaaa atgggataag
    18961 gttatggatt gcaattatgg tacaacttca aaggacctct gggtacgatg gtttgtaaat
    19021 ggtaaggtta atgtatcata caactgtctg gacagacacc ttgaaaaacg gggtaatcag
    19081 atcgctttga tctgggaagg caatgaacca accgaagaca agaagttcac gtataaagag
    19141 cttcatgagc aggtgtgtaa gtttgccaat gttttaaaga agaatggtgt aaagaaaggt
    19201 gaccgtgtga cattcttcct ccccatgatt ccagagcttg caattggtat tttagcctgt
    19261 acacgaattg gtgcagttca ctcagttgtt tttggcggat tttctgcaga agcactgcgt
    19321 gaccgaattc aggactgcaa atctgaggtg ttgatatcat gtgatggtac tttccgagga
    19381 gcaaaagctg ttcctcaaaa agatactgct gataaagcaa ttgctgagtg cccaacggtt
    19441 cgtttgcaca ttgttgtgaa gagggttggc agcaagattc aggtcaactg gaatgataag
    19501 atggataagt ggtatgaaga agagatggct aacgcagatg ctaactgccc ggcagaacag
    19561 atggatgcag aagatcctct gtttattctt tatacatcag gctccacagg gaaacccaaa
    19621 ggtgtaatgc acaccaccgg tggatattta acctatgttg cttacactca caaaatgatt
    19681 tttgattatc atgatggcga tatctactgg tgtacagcag atgttggctg ggtaaccggt
    19741 cactcatata ttgtatatgg gccactggca aatggtgcaa caaccattat gtttgaaggt
    19801 gttccaacat atcctgattt tggaagattc tgggcaattg ttgagaaata taaagtaaat
    19861 atcttttata cagctccaac tgctatccgt gcgttagcaa aagaaggtga tagctgggtt
    19921 gacaaacatg atatttcttc cattcagcta ttaggttctg ttggtgaacc tcttaatcca
    19981 gaggcatgga actggtatta ccataagata ggcaggggtg agtgtccaat agttgataca
    20041 tggtggcaga cagaaaccgg cggtattttg attaccccgc taccaggagc aattgatatt
    20101 aaaccggcta tggcaacagt accattcttt ggtgtagagc catgtattgt tgatgataat
    20161 ggcacagagc ttgaaggtgt atgcaccgga aatctttgta taaagagagc atggccaggg
    20221 cagatgagag gagtttatgg agatccagaa cgatttttta atacatactt tacgcaattt
    20281 cctggtaagt actttacagg tgatggcgct aaacgagaca aagatggata ctatcaaata
    20341 acaggccgtg ttgatgatgt tatcaacgta tcagggcatc gtatgggtac tgctgaaatc
    20401 gaatctgctc ttgttgcaca tcctaaagta gctgaagctg ctgttgttgg atatcctcat
    20461 gagattaagg gacagtcaat ttatgcgttt gtaacgttaa atactggtgt agaaaagagt
    20521 gacgaactta agaaggagct tgttgctcat gtccgtaaag agattggacc tattgcaaca
    20581 cctgaaatga tacaatgggc agatgtcctg ccaaaaactc gttcaggaaa gatcatgcga
    20641 cgaatcttaa agaagattgc tgctggcgaa tttgataaat cgggtatggg tgatacttca
    20701 acgctggctg acccaactgt tgttgataca cttatcaatg atgcaaaaaa attgcagggc
    20761 aagtaagaaa tatttacaat agaaatagaa gagaagtaga aaaaaggctg ccgggtaatt
    20821 ggcagccttt tttaataatc aactatgcga aaaaaatcga ttcagtagat tcaatcatat
    20881 tcagtaccat gcaccaaata ccatgaaaaa aaacggagaa tacattgatg tcaattcaac
    20941 tgggggaaaa actctggagt actgactcaa cactacacca tcaccattca acccctctgc
    21001 gagctttaac gccattatta taataatgtt ttatctcctt catctctgta atcaaatcac
    21061 agtaggggaa taatgttttg gaaagtccgc gaccagttaa aattaattca gtttcatgat
    21121 gttttatttc aaccagatgt ataatatcct cttctgaaag aagtttccac tgcaatgcag
    21181 ttaatatctc atcacatata acgatattaa aggaactatc ggtacataca gctaaacatc
    21241 gatcataagc ttttctggtt aattctttaa aatactcatc atctgttccg ggtatataaa
    21301 agtttttgct tccataagtt tcaatggtga taagatcatg gaatcgtttc agtgcaatac
    21361 gctcacccga tttttttcct tttaagaatt gtatgatgat tgaatgttta ctccacccgg
    21421 cggcacggac agctaatccc aatgctgcgg ttgttttgcc cttaccgttg ccggtataac
    21481 actgtacata cccattcatg atcatgcctt cttctttaat gtgagcatca taacgctgct
    21541 gatgagcacc atacctgcaa ggattaaaaa tgaaagttca aaagagttgg ttaagttttt
    21601 aatgatacct atcagataag gtccaacata tccaccaata ttacccgatg agttaatgag
    21661 ccctacagct cctgctgcgg ctgcaccaga aagaaatgtt gttggatatg accaccagac
    21721 cccaaatggg ccaaatatgc caatggcagc tatgcataca aatagcatgt tgacaacggg
    21781 atcttgtata aatacacctg ctcccaaccc tagagcggca atagcaagcg gtattgcacc
    21841 atggagcctt ttttcgccgg tttttgacga agaatagcca atataaacca ttacaatgag
    21901 tgatactgtc atgggtatgg caaaaagtga accaatagtg atattagacc agcccgatag
    21961 ttcctttata acggttggca tccagtaatt aaatccccag aatccggtta tccataaaaa
    22021 atagatgagt gctaatttta atacctcttt gtcctttaac gcctgccata tagtatagtg
    22081 ctgtgttttt tgtttacttt catctgataa tgcattgaga agatattcct tctctttatc
    22141 agatagccat gataccttca tgggatcatt tgacaggata aagtataaaa gtatgccaaa
    22201 gaaaagtgcc ggaatagctt cacatacaaa taatagctgc catcctttta atccaaatag
    22261 tgatacgtct aataaaaagc ctgcaagtgg tgagccaata atagcagaaa cctgtaacga
    22321 ggtaagcatc aatgctatag ctttaggtcg ctcctgcaat gtaaaccagc gtgggatgac
    22381 actggcatag agtacgggat aaaagcttgc ttcagcgctg cccaataaaa agcgcaatgt
    22441 ataaaaatgc catggtctgg taatgaatat cattaaaaat gaaacaatac cccacgttat
    22501 cattatacgc atgagccagc ctttgggatt ccacttttca gcgataatgg cgcccggtat
    22561 ctcaaagagt atgtagccta taaaaaagat accggcaccc atcccaaaaa tttccggggt
    22621 aaactgtaaa tcctgattca tcgtcaatgc tgcataggca atattaaccc tatccaggta
    22681 tgcaatgatt gagccaataa atagaggtaa taaaacatgt atatatcgtt tcaaggtaaa
    22741 attttttata gcgatagtat ccatagaaca atcatcctgt atctttgtag caaagtatat
    22801 tgtaatgaag tgataatgaa aattgcaatt gtttttatgt aaaaaatatt taaaatagta
    22861 tacttcataa aaatgcttgc aattttaaaa aattatttca gtattgcagt atagggcgat
    22921 tagctcagca ggttagagcg cctgcctcac atgcaggagg tcactggttc gaatccggta
    22981 tcgcccacat ctacacaata catgcaaaat atccctttcg tctgctgtgt acattgtaac
    23041 agaaagttta tcacaggaac tatcgccaaa agccagtccg cagtgatagt ttttcaatag
    23101 tgtaacattg gataaaaata aaccgtcagc atttagtatg gtgtcgtact gtaaagcctg
    23161 tgcaagcgat agtcctttta attttgcaat tgcctctttt attgtccata gtgaaataaa
    23221 ccgtttggtt ttatgcaaca acgagcggtt tacaaactgt tgttcgccta tggaaaagta
    23281 acgtttaaca attttgttgc gatagacagc atgccgtatg gttgattcga tgtcaatgcc
    23341 atgaggtttt tcgcttatgc tgatagcgac agcgttgtca gtgtgggata gtgatagcca
    23401 gtactgtttg ccatcaataa cgatagtatt accgttcagt ggatggtgcg cggttccaga
    23461 taacaattgc aaaaaggctt ttgtggcagt tttgcgtatt actatttcgg ctttgcgatt
    23521 cagtgatgag cactgttgca gcttatgagc atcttcagca tcaaggaaaa aatggtatgc
    23581 atcgtgatgc tggatcacaa aatttttatt gtattgtaca aagaaacact gtaaaggtaa
    23641 actgctcata cttatactgg taataaaaaa attatggcta taataacaaa cagataattt
    23701 ttttcaatat tttttgctat atttattaaa tatgaacaag caggtgagga ttgtttttta
    23761 agcacagtat gttgtaacaa gttttgttac cgatagtacc tttaaagaac aattccactc
    23821 aggatgaaaa tttcaggaga atctatgcga agaacaaaga ttgtatgtac gctgggacca
    23881 acaagtgaag aaaaagaggt tattgtaaac cttgtccgtg cagggatgaa tgttgcaagg
    23941 ttaaacatgt cacattccac ccatcagatg catgaaaaaa gtattgcact gatcagggaa
    24001 atagcaaatg agctgaacac gcctatcggg atactggctg atttgcaggg gccaaaaata
    24061 cgagttggca cattaaaaga aaaggtaatc ctggagccag gacagcagtt tatcttaaca
    24121 acgcgtgatg tccctggcaa tgcaatagca gttagtgttt cgttaaaaga actccccgaa
    24181 tcggtacata aagggcaggt catacttctt gatgatggtt tgcttgaact ggttgttgat
    24241 gaggttgaaa acactgatat ctatactaca gttgtccgtg gtggtgagct aaaagatcat
    24301 aaagggatta atttgcccaa tgcatcaata aaaacaagct caataactca aaaggatatc
    24361 agtgatctgg agtttgtaat aaagcatgat gtagatatgg ttgccttgtc atttgtgcgc
    24421 actgcacagg atgttgttca actcagaagc attatggaag aatttggagg atcaataccc
    24481 attatatcaa agattgaaaa gcatgaagca gttaccaata tagatgatat tattgctgca
    24541 tcgtatggta tcatggttgc gcgtggcgat ctgggtattg aaatccccat agagcaggtg
    24601 cccatagttc agaagatgat cattgaaaaa tgcaatcaac aggcaaaacc ggtaataact
    24661 gctacccaga tgctggattc catgatacgc aatcccatac caacacgagc agaagcaact
    24721 gatgtggcaa atgctgtttt tgatgggaca gatgcgttga tgctttccgg ggaaactgct
    24781 tttggacagt atcctgttga tgcagcagca acaatggcaa agattgctca atatgctgaa
    24841 gactccat
//
LOCUS       DAOSOX010000015        23739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2153_length_23739_cov_8.246031, whole genome shotgun sequence.
ACCESSION   DAOSOX010000015 DAOSOX010000000
VERSION     DAOSOX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23739
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2153_length_23739_cov_8.246031"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="PLX22_02180"
     CDS             <1..1215
                     /locus_tag="PLX22_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT18742.1"
                     /translation="AGFPGIPLAIVYPDKRFILAESRHKRTQFLSIVQNSLQLNNIEI
                     YPHLVTEKSFFEVSGVITRAVESIDETLHRCHHFLQQGNIVLLMKGPSVDEENITLCD
                     SYSKIMDQPYTLPATSYKRRLIVYKKTDGIIKKTYFINKEGRSGDGLAITSGDNKRYR
                     ELKKIITDPRKCSMSTVVGKKIIKELVSSHPQLCRYIVLYDEYAETDTELMQIIADFG
                     HQRIILLKKALFNEIDVLGNNQPILVIDVPPITPWNMDTQGLSLMIPFQDPVNVGAAI
                     RSAVAFGVNNIIICKNAAYPFHPKAIRSSAGAVFHCTLWQGPPLYQHQPDFIELSYPI
                     IALDLEGINIYDFTFPEDAVLLAGVEGQGLHSIKNCIRVTIPIQVVESLNATVALSIA
                     LYEWHKQTTISC"
     gene            complement(1202..2638)
                     /locus_tag="PLX22_02185"
     CDS             complement(1202..2638)
                     /locus_tag="PLX22_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT18743.1"
                     /translation="MAITQLPPNGIEYNKVLSMMKQYRKDDANWKQGKTFSLVYHRDD
                     AHTKFLQKAFELYFDENALNPMAFKSLKKFENEVVKMTANMLHAGPEACGTMTSGGTE
                     SCLLAVKTYRDRARSLHPWIKKPEMIVPESIHVAFEKGAEYFDVKIIRAPLDKNFKVD
                     VNAVKKLINRNTILIIGSAPCYPYGTVDPIAELGSIAHLKKIPLHVDACLGGFLLPFV
                     EKSGYDIPPFDFRVNGVTSMSADVHKYGFSAKGASVIVYRSIDYLRHQFFIYENWPGG
                     VFASPALLGTRPGGTIAAAWAAMVAQGEKGYIKNAKIIMDTSRKIQQGIESIKGMEIV
                     GKPESSVFAYRSIEPDVNTFAIGDILEKKGWHVDRLQRPDALHAMITPIHAKVAQQFI
                     NDVKEAFTTVRKNPELAYEGGAAMYGMISHIPMRKMIRKNIAKMMEGMYGPDGAIPDL
                     SMDETGGEDFAMKAGKWYLKLKDTFSKK"
     gene            complement(2683..3846)
                     /locus_tag="PLX22_02190"
     CDS             complement(2683..3846)
                     /locus_tag="PLX22_02190"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOT18744.1"
                     /translation="MNTAIFLLLICFYLLIPALIIYVCYKFPSINKIGAVLLCYSAGI
                     TIGNIGILPEQAKSIQDISSNIAIILGLPMLLFTINIKTWFRLAGKALLAFTLECIAV
                     VIVVFAGYLLIRHIIPDAWKIAGMLLGVYTGGTPNLAAIKEAFNVDSTTFVIVHTYDT
                     VLSMVYIIFLVTIAQKLFLKFLPPFKVINNENEEDKNAKTGEDIRLYGDIFSKNILKN
                     LVIALLLSIVVVAISLGIAGLLPNQYQTMVVILAVTTIAILLSLIPYVHNLKRTFQFG
                     MYLIYIFCTIVGSMVNVDNIIHINIPLLFYVFLAIFGSLLVHGILCKIFSIDTDTYII
                     TSVAGICSPPFVPVVADALHNKYILLSGITTGVIGYAIGNYLGITLGYVFSSI"
     gene            3987..4640
                     /locus_tag="PLX22_02195"
     CDS             3987..4640
                     /locus_tag="PLX22_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18745.1"
                     /translation="MKKEERIKWDKEQRTARIIEIAQELFFTRGFDATTIDEIAHLAG
                     YNKRTLYHYFKDKEELFLAVALNGLKLFNEKLEEAFNYPLEGHTRLYSMGKAFYNFFL
                     KYPDYFNLIMTYEARNCMYYPVTNSNTMSFYKLECQKIADSDTELIIKAIEYSINDGI
                     IKTTLTPKQLMLILWGQVFGITQIIMIRKDFFKEAYGIDHEELFETFLRETEKALKN"
     gene            complement(4712..4912)
                     /locus_tag="PLX22_02200"
     CDS             complement(4712..4912)
                     /locus_tag="PLX22_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT18746.1"
                     /translation="MPKGTIKWFNEKKGFGFISKEDGSDVFVHYTGIIGNGFRTLKEG
                     QRVEFEIESSDKGPRAVSVKAI"
     gene            5502..6251
                     /locus_tag="PLX22_02205"
     CDS             5502..6251
                     /locus_tag="PLX22_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOT18747.1"
                     /translation="MRIIAISNNKGGVGKTTSTVNIAAALQIIGKKVLVIDIDHQAQS
                     TYHFGYNPHNLTYSIYNILKGEIAYKEILIDRNGLHILPSKNDLKDIEFIPLPAKEFL
                     LKEALENISGYDFILIDCPPSLGILTLNALAFAKEIFVPLQVQFLPFHGMYNLFEAVQ
                     MVKRRLNKEIEITGIIGTMYNPKKQINVEVIDETKKRLPGKLFETLIRENVLLQEAPS
                     WGKTIFEYKPNSNGADDYMNLTKEILERSPD"
     gene            complement(6255..7325)
                     /locus_tag="PLX22_02210"
     CDS             complement(6255..7325)
                     /locus_tag="PLX22_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOT18748.1"
                     /translation="MENYIDKPADIREGEDIDKHILFDYLVNNVSDIKGTIEIKQFPG
                     GFSNLTYFIKIGDRELVLRRPPFGKKAKTAHDMHREFVILKALKPVFPYCPAPLVYCD
                     DESVIGAPFYVMERIKGIILRKELPQGLSYTPDEFKYLCEQLLDIQCLLHSLHYKELG
                     LENFGKPDGYIERQVHGWINRYNDALTPDAPSYTEIITWLKEKLPETPTRAAIIHNDY
                     KFDNVVLDNKNPKKIIGVLDWEMATIGDPVMDLGNSLAYWVEKEDPEDMQLIRLMPTH
                     LDGALTRKELVNRYHEKSGIDMSDFDYYYCFGLFRLAVIAQQIYYRFYHGQTKDKRFS
                     TLILAVQILEKQAKRVIEKSHL"
     gene            complement(7750..8478)
                     /locus_tag="PLX22_02215"
     CDS             complement(7750..8478)
                     /locus_tag="PLX22_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT18749.1"
                     /translation="MKAEKSDNFNELFIDNYNNFRKSIKIVLDEIVTKLYDDNKQLIK
                     VKKSDQSINNLKRIINTAISISNKKGYAAMSMRELSRASSLSIGALYSYFPSKDELLH
                     IIQQYGRAAVEKTLSSALSKASSTKDKLYNFIHAHIFISEALKDWFYFSYMETRYFTG
                     IEYNKTIESELFTESLIDSIIKDGIKKSECKSNLNSSLIASVTKAMMQDWYLKRWKYK
                     RRNILPEEYALECFNIIYNYIHNG"
     gene            complement(8456..9664)
                     /locus_tag="PLX22_02220"
     CDS             complement(8456..9664)
                     /locus_tag="PLX22_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT18750.1"
                     /translation="MDFEISDKMKTILELIDEFVDKELIPLEQEFVSKDIKAMMPVLE
                     EKRNMVKKMELWAPQVPKEYGGMGLDLVEHGLVSESLGRTPLGHYVFGCQAPDAGNIE
                     ILIKYANDEQREKYLLPLIQGKIRSCFSMTEVDLPGSNPVLLDATAVKDGNQYVINGH
                     KWYSTAADGSQFSIVMAVTNPEAPVYLRASMIIVPTDTSGFNLVRNIPVMGHEGSGYF
                     SHGEILLQNCRVPVENLLGPEGQGFVIAQERLGPGRIHHCMRWLGICKRSFDLMCSRA
                     NKRIIAPDGKTLASRQIIQEWVAECAAEIQAARLMTLQAAWKIEHLGQKEARIDVSLI
                     KFYVANVMQKVVDRALQVHGGLGMTDDTILAFFYRHERASRIYDGADEVHKTAVAKRI
                     LSGYEGREIR"
     gene            complement(9689..10408)
                     /locus_tag="PLX22_02225"
     CDS             complement(9689..10408)
                     /locus_tag="PLX22_02225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT18751.1"
                     /translation="MSEILLVRHGQGSFGQGDYDNLSEKGQKQSLILADYLYNYRYNN
                     KQIDVIYSGTLKRQQHTAQILIDYFLSKGMQVPSLQTDSRLNEYDSINIIKTIFPILL
                     NDDPHLDEEIKQIYTDKKAFQRVFEKVMLYWITTKTSFEGIESYEHVKKRVNSFVTEI
                     TKNHGRGKNIVLVSSGGIIGTTVQMVLGFSDETTMQLTWQIVNTSMTHLRYNDKALVL
                     ASFNAYPHIELLQDASMKTYR"
     gene            10623..11387
                     /locus_tag="PLX22_02230"
     CDS             10623..11387
                     /locus_tag="PLX22_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT18752.1"
                     /translation="MSIPYKILVCIKQVPDVNAKIYLEKTKPYYDEKTVYVINRYDEY
                     ALEEALQIKDKGLTHYIHTISSGPERVQKALRRSLEMGADEAYHIVTGDDDCAIPYYV
                     ARCITEFAKKEYDIIFTGVMSQDAMNQQTGQLIASIMGYNYATGVTSIQTNDDEITVT
                     RELDAQTSMQYVLKIPCVVTVQSSSTMPRYPSLSNVLRAKKQHIDIIKPIMSSQVQYH
                     SFYTIPQKTTECEFIQGSTDQKVVELYNILHRNGVV"
     gene            11391..12347
                     /locus_tag="PLX22_02235"
     CDS             11391..12347
                     /locus_tag="PLX22_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT18753.1"
                     /translation="MSILIIADIKNNQINPATAELFTAANIIAKDITTPVYLLIPCND
                     AKITSNYRPDVTTIVIGMGIYHYNAPGLSKAIASIAHDIEAHYIIGLHTPFTIDFLPS
                     VSIEYNAHCVTQVHTIEYDEHLTLARGSYSGKLDQFVSIVDDKPVVASITPGSFDEYG
                     AKDADDLINKVIEINIDTAIKQWCTFIISENKDSSLDEADIIIGVGKGIGSKDNLTLI
                     YNFAKFFPHSAVAGSRIVCDYGWLPYSKQVGITGKSISPKVYIACAISGSSQHIAGIK
                     GAKTIIAINKDPYAPIFNVSHYGIVEDIFTFIPQFLEYIQRL"
     gene            12693..13283
                     /locus_tag="PLX22_02240"
                     /pseudo
     CDS             12693..13283
                     /locus_tag="PLX22_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            13372..14217
                     /locus_tag="PLX22_02245"
     CDS             13372..14217
                     /locus_tag="PLX22_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18754.1"
                     /translation="MSQPDDFTLDDDLFKLDDEIDIQDNIPENKPEANNNTVLDSGKD
                     YGDSSRKRSSRDFQPDMDALLITAQSSMIIEGMKKYTERDFSSNNLPIYLEALNGVNL
                     YIKILDRNPKNYHKLKSVINADQDCQEVEKIAFDLYKKMFGTVPESDQEKLVAFEKFS
                     SLFKDAVNRATINASMKEIKKYFLLSGGLDEIKVSKLIAQNDEELAKDISNLQRYIAM
                     GLAYIEKGNIEIIKGLKGRDLNNYIIRGSQLLTFYFKKINNDRLADYYSRINSIYSKY
                     FVIKD"
     gene            complement(14364..15515)
                     /gene="hemW"
                     /locus_tag="PLX22_02250"
     CDS             complement(14364..15515)
                     /gene="hemW"
                     /locus_tag="PLX22_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT18755.1"
                     /translation="MIHSGIYIHIPFCNTKKCDYCDFYSIPCNPSHSNFQQIKHAYIN
                     ALVSEIIHYSSELNNYLVDTIYIGGGTPSLLQCSDYNVLLTVLHDYYAIASEVEITLE
                     CNPDDYSKNYIQQLSHIGFNRFILGVQTLDTRAHKYIGRKSMLCTMEEIQEFCTIPDI
                     IPCIDIIAGIPVQSVTNYRSMLNSIIQCNPKHISAYMLTYETNTPLRLRTKNPLQYAN
                     FQRIMFQKTIDALVASGYNHYEISNFSIPGYESKHNLKYWNFESYIGFGSGAHSFYNN
                     KRYYNGTIDDYMNDAIASRTEDIRTQPDSVLEYCMNKLRLKQGFMINDMRNKTGYLPA
                     ESFYQSIHRLIDKSLLIMQTCDGDICIQCTDEGMFMLDTILLELASSVS"
     gene            complement(15512..17128)
                     /gene="nagZ"
                     /locus_tag="PLX22_02255"
     CDS             complement(15512..17128)
                     /gene="nagZ"
                     /locus_tag="PLX22_02255"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOT18756.1"
                     /translation="MKIKPMTAVSLLLVLVQLIGCKQPKDYITAGKIASGLANDVLLG
                     QMLIIALPSGVVDDKTIAIIKKYKPGGVIIFGYNLTDGSCKTFCNDLQNIAMEHIGIP
                     LFIAIDQEGGRVKRIQNGVTQFPGNFALGVINNPSKVYRMAQIVGMQLRIAGINMNFA
                     PVLDVNNNFNNPVINIRSFSCIPQVCANLGAAYIQGLQQSRCIAVAKHFPGHGDTYQD
                     SHITLPVINKTHASLNKTEFVPFKKAISSGVECIMTAHIAFPQLGIVKPATMSEYFLN
                     HLLRKKMKFDGLIITDDLEMKGISGYYSQGTAAVASVKAGADIVLISSYEDHVQDIIN
                     SLSNAMLKNEVSKDRIVSTVKKIIEVKMRYGIMTIKKNKIYNSNFNGGDSYLEMMQQA
                     DTLNTELSRESIVYHSKNDDFTTFIPAENRKATILTTDKEVEDIFKAINADVIHSIQL
                     LKNKKILFIDSGCYTTKQLQSIIKTAKQNDCNTLLLVTGNPYPYLKEFGEYPLLMSFS
                     DTHESLHQMALCLSGEFTPKSSHPCLMIDK"
     assembly_gap    17980..18079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18080..18296
                     /locus_tag="PLX22_02260"
     CDS             <18080..18296
                     /locus_tag="PLX22_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18757.1"
                     /translation="KYSSKAEKGLSVRVNGQQISLKGIGAGLKIMCAFMDSITIDLID
                     KEKMISDTVSKVTDGTILSMHRKRRYK"
     gene            18296..19036
                     /locus_tag="PLX22_02265"
     CDS             18296..19036
                     /locus_tag="PLX22_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOT18758.1"
                     /translation="MRPEKAYKNMEFLNSREARIIRIMCEYIEPEKRFKHYKIKHTVV
                     FFGSARTQPDDPLTMQYYHDACKLSYLLAQYGKELEAVHDGFVICTGGGPGIMEAANK
                     GANEAGVYSIGLNISLPFEQIPNQYISQELNFEFHYFFMRKLWFLYHAKAIVVFPGGY
                     GTLDELFETLTLVQTNKLQKQDIPIVLYDENYWNKLINFQMLIDCGYIDKKDFDLIHF
                     CNSPEEGIEYLKPRLKGLIEKINHSIGE"
     gene            19043..20239
                     /gene="argJ"
                     /locus_tag="PLX22_02270"
     CDS             19043..20239
                     /gene="argJ"
                     /locus_tag="PLX22_02270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT18759.1"
                     /translation="MEKIKELSGGLENVTIFTYAAIECGIRYENRLDFSLIYSDKPCW
                     AAGAFTTNKFCAAPVKLCRERIKNTIHAVIINATNANAATGKQGYDNAKLLTAEVAKI
                     LSIDEKTVLMASTGIIGVQLPVEKMKNNLPILVKNLNSESGKLLPTAIMTTDTFPKQY
                     ALSFETSTGIYTIAGIAKGAGMIAPDMATLLCFVVTDFPLQQRLLQKLFTSSIAVSLN
                     AITIDGDMSTNDTAILLSPAIHTKHTHADIDIFNNALTMMMKRLAYLIVSDAEGATKC
                     VTIKVVGAANDSDAKIAAKSIAESLLVKTAIFGNDPNWGRIACAAGYSGAELREDKLS
                     IYFEDISLFKNGSPVNFDKDRLIDILKRKEYTIIIDFGIGKSLFEYLTSDLTYEYVKI
                     NAEYST"
     gene            20382..21464
                     /locus_tag="PLX22_02275"
     CDS             20382..21464
                     /locus_tag="PLX22_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18760.1"
                     /translation="MAEFKRIVYSIIMFFIFISLVNGQTIDKHKIIIYYFEDESSLQN
                     YRYYSYIIPDNLAIELQNHVEYEVTVLPVTFKYISDDMPQEEKENTITILSTRAKEVN
                     ARFVIIGSYKVEKKRIFIQTQIYNTVTGELTDAAEVEETIGAIVYEIIEKITLKINSQ
                     LQKTLDVEKEKIALSPYKDFYSTFQNSYFGYSYSYVFFTGSWENLYNDPKMSSVYIGF
                     PLSKMYASNSVFIKNIYIVPTWQFYSTNNHDLKNMNDSYLSIYTGSIALQYQYSLSPF
                     MIVFASLGAGVSYSDMRFVLASEGGPFSPETKTDSTDPYFGANLGAIFYIHNIGISGG
                     LGYNRIFYKGDDLASFVMSFGILYRL"
     gene            21590..22087
                     /locus_tag="PLX22_02280"
     CDS             21590..22087
                     /locus_tag="PLX22_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18761.1"
                     /translation="MKSKIVYSHNDYWCKHQIYDAIAYGCDMVEADVIYMNNKIILSH
                     SWRPFSFMCYGNLEDNYLKPLSQYCKDNPDKTMWMYVEFKDSNEHIIELLHKLFKQYQ
                     TKNLRYTVSALGSKWYQKKRYETAMQFYKKYKDEFSLLWKETELEQQYQVISVDLYPQ
                     SIWHF"
     gene            22084..22323
                     /locus_tag="PLX22_02285"
     CDS             22084..22323
                     /locus_tag="PLX22_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18762.1"
                     /translation="MISQYRGSLSVVGLQLPERNVIGINAIILLFRGKANSHCETIVY
                     KMSLVDAKNILYVLYERSDSLGLPYLNMTLWEMYC"
     gene            22509..22853
                     /locus_tag="PLX22_02290"
     CDS             22509..22853
                     /locus_tag="PLX22_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOT18763.1"
                     /translation="MKLIVKKWFILSIAIYLASVLLNGFSAETATAIIIASALLGIFN
                     VFIKPVLIFITLPINLLTLGLFTFVINALLLYMVSSIVKGIYLDSFWSALLASIIISI
                     LSMIINWIFADR"
     gene            22886..>23739
                     /locus_tag="PLX22_02295"
     CDS             22886..>23739
                     /locus_tag="PLX22_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT18764.1"
                     /translation="MFIVSIGAGKNQLPLIRACKDLQCKIVGVDINPHAEGFPLCDFK
                     IIESIYNYHEIYQSLKELLVFDDIAAIVTRSYGEALRTVSYLCQQFKIPHINYAMIDY
                     CIDKSKFRTIANKTITMPHCFTMGKNDKGNILTLSDITFPCIIKPSQGHAKHNVQLID
                     SVQHLKQSIASMKHSTIMVEEYIEGDEIIVIGFVINKKFYICDISDKVLNAKPYFVDR
                     MHLLPSTYYHYHNQLTSIGQQIADLFLLESTPLLMECVISNNTIYVIEAACEFGGEYL
                     ADYAIPAR"
BASE COUNT         7930 a         4015 c         3930 g         7764 t
ORIGIN      
        1 gcaggttttc ccggcattcc attggcaata gtttatccgg ataaacgctt catattggct
       61 gaaagccggc ataaacgcac acaattttta tctattgttc aaaattcttt gcaactcaat
      121 aatattgaaa tataccctca ccttgtaacc gaaaagtcat tttttgaagt aagcggtgtt
      181 attactcgtg cagttgaatc aatagatgaa accctgcatc gatgtcatca ttttttgcaa
      241 caggggaata ttgttttatt gatgaaaggg ccatcagtgg atgaagaaaa tataactttg
      301 tgcgatagtt attcaaagat aatggatcaa ccgtatacat tgcctgccac atcatataaa
      361 agaagactta ttgtttataa aaaaacagat ggaataataa aaaaaacata ttttattaat
      421 aaagaaggaa gatcaggcga tggattggca ataacctccg gggacaataa acgttacagg
      481 gaattaaaaa aaattattac tgacccaaga aaatgcagca tgtcgacagt tgtcggaaaa
      541 aaaataatca aagagcttgt ctcttctcat ccacaattat gtcgctatat tgtattgtat
      601 gatgagtatg ctgaaaccga tactgaactg atgcagataa tcgctgattt tggacatcaa
      661 agaattattc tattaaaaaa agcacttttc aacgagattg atgtgctggg caataatcag
      721 ccaattctgg ttattgatgt gccacccata acaccatgga atatggatac tcagggacta
      781 tcgctcatga ttccttttca ggatccagtc aacgttggtg cggctatacg aagtgccgta
      841 gcatttggtg ttaacaatat tattatatgc aaaaatgccg catatccctt tcatcctaaa
      901 gcaatacgtt catcggcagg tgcagtgttc cactgtactt tatggcaggg tcctccactt
      961 tatcaacatc aacctgattt catagaacta tcgtatccaa ttatagcatt agaccttgaa
     1021 ggaattaata tatacgattt tacttttcca gaagatgcag ttttacttgc tggtgtagaa
     1081 ggacagggat tgcacagcat aaaaaactgt atccgtgtta ccatacccat acaggtggtt
     1141 gagtccctca atgcaacagt agcattatca atagcactat acgaatggca taagcaaact
     1201 actatttctt gctaaatgta tctttcagtt taagatacca tttgcccgct ttcatggcaa
     1261 aatcttcacc acccgtttca tccatactta aatctggtat tgcaccatca ggaccgtaca
     1321 tgccttccat catttttgct atgtttttcc gaatcatttt acgcatcggt atatgggaaa
     1381 tcataccata cattgccgca ccaccttcat aggctaattc aggatttttc cttactgttg
     1441 taaaagcttc tttaacatca ttgataaact gttgtgcaac ctttgcatga ataggggtta
     1501 tcatagcatg gagagcatcg ggacgctgga gcctgtcaac atgccatccc tttttttcta
     1561 agatgtcgcc aatagcaaat gtattcacat caggctcaat cgaacggtat gcaaaaacac
     1621 tggattcagg tttgcccact atttccatac cttttattga ttcaatcccc tgctgtattt
     1681 ttctggaagt atccataatt atttttgcat tttttatgta tcctttctca ccctgtgcta
     1741 ccattgctgc ccaggccgct gctatagttc caccaggcct tgtgcccagc aaagcaggtg
     1801 acgcaaaaac accaccgggc caattttcat aaataaaaaa ttgatggcgc aaataatcaa
     1861 tactacggta tacaatgact gaagcacctt tagcactaaa tccatattta tgtacatcag
     1921 cactcattga ggtaacacca ttcactctaa aatcaaatgg ggggatatca tagcctgatt
     1981 tttcaacaaa tggcaataaa aacccgccaa ggcatgcatc aacatgcaaa ggaatttttt
     2041 ttaaatgcgc aatggaacca agttcagcaa ttggatcaac agtgccatac ggatagcatg
     2101 gagctgaacc aatgataagt atggtattgc ggtttataag tttttttaca gcattcacat
     2161 ccaccttaaa atttttatcc agaggtgcac gtataatttt tacatcaaag tattctgctc
     2221 ctttttcaaa agcgacatgg atgctttcag gaacaatcat ttccggtttt ttaatccacg
     2281 gatgtaatga acgtgcccta tcgcgatagg ttttaacagc aagcaagcaa ctttcagttc
     2341 cacccgatgt catggtacca caggcttctg gacctgcatg cagcatgttt gcagtcattt
     2401 ttaccacttc attttcaaac ttcttaaggc ttttaaaagc catgggattt aacgcatttt
     2461 catcaaagta tagttcaaat gctttttgca aaaatttagt gtgtgcatca tctctatgat
     2521 aaacaaggct gaatgttttt ccctgtttcc agttagcatc atctttccga tactgtttca
     2581 tcatggacaa aaccttattg tattcaatac cgtttggtgg aagctgtgtt attgccatgc
     2641 gatacctcct ataataataa ttatttatta ttattatttt acttatatag aactgaaaac
     2701 atatcccaat gttatgccaa gataattgcc aatagcatag ccaataacac cggtagtaat
     2761 accggacaat aaaatgtatt tattgtggag tgcatcggca accactggaa caaatggtgg
     2821 tgaacatatg cctgcaactg aagtgatgat ataggtatca gtatcaatgg aaaaaatctt
     2881 acacaatata ccatgcacca ataaactccc aaaaattgct aaaaacacat aaaacaatag
     2941 cggtatatta atatgaataa tattatcaac atttaccatc gaaccaacaa tagtacaaaa
     3001 aatataaata agatacatcc caaactgaaa ggttcgttta agattatgta catacggaat
     3061 aagtgaaagc aaaattgcta ttgtcgttac tgccaaaatg acaaccatcg tttgatattg
     3121 atttggcaac aaaccggcaa tacccagtga aattgcaacc actacaattg ataaaagcaa
     3181 agctataacc agatttttca aaatattttt ggaaaagata tcgccataca atctaatatc
     3241 ttcaccagtc tttgcatttt tatcttcttc attttcatta tttattactt taaaaggcgg
     3301 taaaaatttt aaaaaaagtt tttgtgcgat agttactaaa aatataatgt ataccattga
     3361 aagtacagtg tcataggtgt gaacaattac aaaagttgtt gaatcaacat tgaatgcttc
     3421 tttaatggca gctaaatttg gtgttccgcc tgtatatacc cctaaaagca tcccggcaat
     3481 cttccatgca tcaggaatta tatgccttat cagtaaatat ccagcaaata ctacaataac
     3541 taccgcaata cactccagtg taaacgccag tagtgcttta ccggctaacc tgaaccatgt
     3601 tttaatattt attgtgaaga ggagcattgg caaacctaaa atgatagcta tgttggagct
     3661 tatatcctgt atagactttg cctgttcagg caatatgcct atatttccta tagtgatgcc
     3721 tgcactataa cataacaata cagcacctat tttattaatt gatggaaatt tatagcatac
     3781 gtaaatgatg agcgccggaa tcaaaaggta aaaacaaatc aataataaaa agatagcagt
     3841 attcatacca cctccattta tttcactata gtgaatatta tttcacttta gtgtaattgt
     3901 caactgtttt ttgtttgaca tatatatcat aaagattttt ttaataccag attattaatg
     3961 acgtaaagga atataaagtt actgctatga aaaaagaaga aagaataaaa tgggataaag
     4021 aacaacgtac agccagaatc attgaaatag cacaggaatt gttttttacc aggggatttg
     4081 atgcaaccac aatagatgaa attgcccatt tagcaggata taataaacgt actttatatc
     4141 actatttcaa agataaagaa gagctgtttt tagctgttgc actgaatgga ttaaaattat
     4201 ttaatgaaaa attggaagag gcattcaact atccattaga aggtcataca aggttataca
     4261 gcatgggaaa ggcattctat aatttttttt tgaaatatcc tgattatttt aatcttatca
     4321 tgacatacga agcacgtaat tgcatgtatt atccggtaac caacagcaat acaatgagct
     4381 tctataagct tgagtgtcaa aaaattgcag atagtgatac tgaattaata ataaaagcta
     4441 ttgaatatag tattaatgac ggaataatca aaacaacatt aacaccaaaa caattaatgc
     4501 tcatattatg ggggcaagtt tttggcatca ctcagattat aatgatacga aaagactttt
     4561 ttaaagaagc atacggcata gaccatgaag aactttttga aacattttta cgtgaaacgg
     4621 aaaaggcatt aaaaaattaa aatgatatag taattaaaaa agccgccaga aggcggcttt
     4681 tgcaaagtgg ttttctaaaa tgaataattc attatatcgc tttaacggaa acagcccgcg
     4741 gacctttatc gctgctttcg atctcgaatt caaccctttg tccttctttt aatgtcctga
     4801 aaccgttacc tataataccg gtataatgca caaaaacatc actaccgtct tctttggaaa
     4861 taaatccaaa accttttttt tcgttgaacc acttaattgt acctttgggc atgtgaaaac
     4921 tccttaaaaa ataaaatacg gcgtaaagcc tttcagtatg ttgattttgc ttttaacctc
     4981 taaaaaacct catattgaaa tttcacaaga gacaaaacca aaatacaatt catactatta
     5041 tacatatata ctattacttc ttattgttgt caacatttta ttacaattta tttaattttt
     5101 tttataaaac ctataattca cataaaacct attttattga ttctttacct atttggtaaa
     5161 ttttaaaaaa atagcacaca tcaaaccata ttgctccata tattcataaa aattttttac
     5221 ttgtatttta cagtgattaa gcatagctgg aattcaatat ttattgtact gtaatcttga
     5281 tattattttt gaataatccc catttttatt tttaatatca ttgtttttaa ataattatgg
     5341 caaaataaat tttttataat tgcaaaaaac atagcaattc atatataaaa gtattaataa
     5401 tttttagtat aatacgtttg ttaattgtgc gataaataaa caaggatatc taatcgatgc
     5461 aataattatg agttttataa aaaatattaa ggattaatta catgagaata attgctattt
     5521 caaataacaa aggaggtgtt ggaaaaacaa caagtacggt aaatattgca gcagcactac
     5581 aaattattgg aaaaaaagtt cttgtcattg atattgatca tcaggctcag tcaacctatc
     5641 attttggtta taatccacat aatctgactt actcaatata taatatcctg aagggtgaga
     5701 ttgcgtataa agaaatctta attgatagaa atggtctgca tattttacca tctaaaaatg
     5761 atttaaagga tattgagttt attccacttc ctgcaaaaga atttctgtta aaggaagctc
     5821 tggaaaatat ttctggttat gatttcattt taattgattg cccaccctct ttaggaattt
     5881 taactttgaa tgcccttgct tttgcaaaag aaatttttgt tcccttgcag gttcagtttt
     5941 taccatttca tggcatgtat aacctttttg aagcagtgca gatggtaaaa cgcagattaa
     6001 ataaagaaat agaaataacg ggtataatag gaaccatgta taatcctaaa aagcaaataa
     6061 atgtagaagt aatagatgaa acaaaaaaac gtttgcccgg taaattgttt gagacattaa
     6121 ttcgtgaaaa tgtattgtta caggaagcgc caagctgggg aaaaacaatc tttgaatata
     6181 aaccaaacag caatggagct gatgattata tgaacctgac aaaagaaata ttagaaagaa
     6241 gtcccgatta aaatctataa atgtgatttt tcaattaccc gtttagcctg tttttcaagt
     6301 atttgtaccg ccagaatcag ggtagaaaaa cgcttatctt tagtttgacc atgataaaac
     6361 ctataataaa tttgctgggc tataactgct aacctgaata aaccaaaaca gtagtaataa
     6421 tcaaaatcgc tcatatctat gcctgatttt tcatggtatc tattgaccag ttctttccgt
     6481 gttaatgcac catccagatg ggtaggcatt aaacgaataa gttgcatatc ctctggatct
     6541 tctttttcca cccagtatgc aagtgagttc cctaaatcca tgacagggtc accaatcgtt
     6601 gccatttccc aatctaatac accaattatt ttttttggat ttttgttatc aagcaccaca
     6661 ttgtcaaatt tatagtcgtt atgaataata gctgctctag tcggcgtttc aggtaatttt
     6721 tctttaagcc acgtaattat ttcggtatat gatggtgcat caggggtcag agcatcattg
     6781 taacgattta tccatccatg tacctgtcgc tcaatataac catcaggctt tccaaaattt
     6841 tccagtccta actctttata atgtaaagaa tgaagcagac actgtatatc cagcagctgc
     6901 tcacataaat atttaaattc gtcaggtgta tagctcaatc cctgtggtaa ttctttacgc
     6961 agtattattc ctttgattct ttccataaca taaaatgggg caccaataac ggattcatca
     7021 tcacaatata ctaatggagc tggacaataa gggaataccg gcttcaatgc ttttaatatt
     7081 acaaattcac gatgcatatc atgagctgtt tttgcttttt taccaaaagg aggcctcctt
     7141 aatactaatt ctctatcgcc aattttgata aaatacgtca ggtttgaaaa accacctgga
     7201 aattgtttta tttcaatagt tccttttata tcagaaacat tgttaaccag ataatcaaac
     7261 agtatatgtt tatcaatgtc ctcgccttca cgaatatctg ctggtttatc tatataattt
     7321 tccatacaat aaactccttt gaaaagaatt attaacttct gtcattacgg gcttgactcg
     7381 taatgcaaat atgtaacttt aattcctaaa accattttaa agagatgatt cattataaca
     7441 cgcacaacat tctcatattg agggatacag cattttatgt cgttctgaaa gagcgataac
     7501 aactgaagta tctccccttt ctcaaaacaa tgagattctc cactccgttc agaatggcac
     7561 attgccactt tgtcattctg agggacacag cctcgtagaa tctattcgtt tatacaggag
     7621 attcttctat accacctaac atgactcaac tttgccttat aatgaggttc acgatacgct
     7681 tttatctgct atggatgcca tacacggaat aacagcaact cctccttcta taaataaatt
     7741 atataaaatc tacccgttat gtatatagtt ataaataata ttaaaacatt ccaatgcata
     7801 ttcttcgggt agtatatttc ttcttttata tttccagcgc ttaagatacc aatcctgcat
     7861 cattgcctta gttactgatg caatcaatga gctgtttaaa tttgatttgc attcactttt
     7921 ttttattcca tcttttatga tactatcaat caatgattct gtaaaaagct cactttcgat
     7981 agttttatta tattcaatcc ctgtaaaata tcgtgtttcc atatatgaaa aataaaacca
     8041 atctttcaat gcttcgctta taaatatatg tgcatggata aaattatata atttatcttt
     8101 tgtggatgaa gccttgctta atgcactcga caatgttttt tcaactgcag ctcgtccata
     8161 ctgttgaata atatgtaaaa gttcatcttt agaaggaaaa taagaataga gagctcctat
     8221 gcttaacgat gaagcacgtg agagctctcg catactcatt gcagcatagc cttttttatt
     8281 acttatacta atagcagtgt ttataatcct tttaagatta ttgatggatt gatcagactt
     8341 tttaactttg ataagctgtt tattatcatc atacagtttg gtgacaattt catccagaac
     8401 gattttaata ctcttcctga aattgttgta attatcaatg aataattcat tgaaattatc
     8461 tgatttctct gccttcatag cctgacaata tcctttttgc cactgcagtt ttatggactt
     8521 catctgcacc atcatatatg cgcgaagcac gttcatgacg gtagaaaaat gcaagaatgg
     8581 tatcatcagt catgcccaaa ccaccatgca cctgtagcgc cctgtcaact accttctgca
     8641 ttacatttgc cacataaaat tttatcagtg aaacatctat tctggcttct ttttgtccta
     8701 agtgctctat cttccacgca gcctgtaatg tcataagcct tgctgcctgt atttcagcag
     8761 cacattcagc tacccattcc tggattatct gtcgggaagc taaagtttta ccgtcaggag
     8821 caatgatacg cttattagcc cgtgaacaca ttaaatcaaa tgatcgttta catataccca
     8881 gccatcgcat acagtggtga atccgaccag gacctaaccg ttcctgagct ataacaaaac
     8941 cctgtccttc tggaccaagt aaattttcta ccggaacccg acagttttgt aacaatatct
     9001 cgccatggct aaaataacca ctaccctcat gtcccataac tgggatattt cgcaccagat
     9061 taaaacctga tgtatctgta ggaactataa tcatacttgc ccttaaataa actggagcct
     9121 ccggattggt gacagccatg acaatagaaa attgagaacc atcggcagca gtcgaatacc
     9181 atttgtgccc atttattaca tattgattgc catcttttac tgcggttgca tccagtaata
     9241 ccggattgga ccctggcaga tcaacttcgg tcattgaaaa acagcttcgt attttgccct
     9301 gtattaacgg taacaaatat ttttcacgtt gttcatcatt ggcatattta atcaatattt
     9361 caatgttacc tgcatccggt gcctgacaac caaacacata atgtcccagc ggagtcctgc
     9421 ccagtgattc tgaaacaagc ccatgctcta ctaaatcaag acccatgcca ccatattctt
     9481 ttggaacctg aggtgcccat agctccattt ttttaaccat atttcgtttt tcttccagaa
     9541 caggcatcat tgctttaata tctttactta caaactcctg ctctaaaggg ataagctctt
     9601 tatccacaaa ttcatcaata agctctaaaa ttgttttcat cttatcagaa atttcaaaat
     9661 ccataaaacg cctccattaa aatatagttt acctgtacgt tttcatcgat gcatcttgta
     9721 ataactctat gtgaggatat gcattgaatg aagccaatac cagagcttta tcgttatatc
     9781 gtagatgtgt catcgatgta tttactattt gccatgttaa ctgcatggtg gtttcatcac
     9841 taaaaccaag aaccatctgc acagttgttc caatgatgcc tcctgaagaa accaatacaa
     9901 tatttttccc tctcccatga ttctttgtta tttcagtgac aaagctgttc accctctttt
     9961 taacatgttc ataggattca atgccttcaa atgatgtttt ggttgttatc caataaagca
    10021 taactttttc aaaaacgcgc tggaaagctt ttttatcagt atatatttgt tttatctctt
    10081 catcaaggtg cggatcatca ttaagcaata tgggaaatat ggtttttata atattgattg
    10141 agtcatactc atttaatcgt gaatcagttt gcaatgaagg aacctgcatg ccttttgata
    10201 gaaaataatc aataagtatt tgtgctgtat gttgttgcct ttttaatgtt cctgaataaa
    10261 ttacatcaat ttgtttattg ttatagcgat agttatataa ataatcagct aaaataaggg
    10321 attgtttttg ccctttttca gacaaattgt cataatcacc ctgtccaaaa gaaccctgac
    10381 cgtgacggac taatagtatc tcactcataa ttcaccagat taaaaagatt ttgaattatt
    10441 aatagcatta ttaactccca tacataaatg tcaagaaaaa aataacgaac gttcgttatt
    10501 tttattgatt tttttaaaat atttttatat aatggtctca tcactaagaa ataaaattac
    10561 agggagagtt gtttgctata tgttcaattg ttcaataatg ccatacagga gatgaagcta
    10621 ttgtgagtat accatacaaa atattagtgt gcataaaaca ggtacccgat gttaatgcta
    10681 aaatttatct ggaaaaaaca aaaccgtatt atgacgaaaa aaccgtgtat gttattaatc
    10741 gatatgatga atatgcactt gaggaggctt tgcaaataaa agataaaggt ttaacacact
    10801 atattcatac catttcatca ggaccggagc gagtacaaaa ggctttgcgt cgctcacttg
    10861 aaatgggcgc tgatgaggca taccatattg taaccggaga tgatgattgt gctataccat
    10921 attatgttgc acgatgtata actgaatttg caaaaaaaga gtatgatatt atttttactg
    10981 gtgttatgtc ccaggatgca atgaatcagc aaaccgggca acttattgca tctatcatgg
    11041 gatataatta tgctaccgga gtaaccagta tacaaactaa tgatgatgaa ataacagtta
    11101 ctcgtgaact ggatgctcaa acttcgatgc aatatgtttt aaaaattccc tgtgttgtta
    11161 cagttcaatc aagcagtacc atgccgcgtt acccttcgct ttcaaatgtt ctccgtgcaa
    11221 agaaacaaca cattgacatt ataaaaccca taatgtcatc gcaggttcaa tatcatagtt
    11281 tttatacaat tcctcaaaaa actactgaat gtgaatttat tcaaggaagc accgatcaaa
    11341 aggttgttga attatataac attttacatc gtaatggagt ggtttagatt atgagtatac
    11401 ttatcattgc agatattaaa aacaaccaga taaatccagc aactgcagaa ttatttaccg
    11461 ctgccaatat aatagcaaag gatataacta cacctgtcta tttgcttatt ccatgcaatg
    11521 atgcaaaaat taccagtaac tatcgcccgg atgttacaac aatagtaatt ggcatgggca
    11581 tctatcatta taacgcacca gggttaagta aagcaattgc atcgatagct catgacattg
    11641 aggcacatta catcattgga ttgcatacac cgtttaccat tgattttttg ccatcagtaa
    11701 gcattgaata taacgcacac tgtgtaacgc aggtacacac catagaatat gatgagcatc
    11761 tcacattggc acgaggaagc tactctggta aattagacca gtttgtttct atagttgatg
    11821 ataaacctgt tgttgcaagt attacaccag gaagctttga cgagtatggt gcaaaagacg
    11881 ctgacgattt aattaataaa gtaattgaaa tcaacataga tactgctata aaacaatggt
    11941 gtacatttat aataagtgag aataaagatt catcattaga tgaagccgat attattattg
    12001 gtgtggggaa aggtattgga tcaaaggata atttgacatt gatttataat tttgcaaaat
    12061 ttttcccaca cagtgccgta gctggttcac gtattgtgtg cgactacggt tggttgccat
    12121 atagcaaaca ggttggcatt acgggaaaaa gcatatcacc aaaggtttat attgcatgtg
    12181 caatatcagg atcttcgcag catattgcag gaattaaagg agcaaaaaca ataattgcca
    12241 ttaataaaga cccctatgct cccattttta atgtttcaca ctatggtatt gttgaggata
    12301 tttttacatt tataccacaa tttttagaat atattcaacg attataaata atcataatac
    12361 gcttgacaaa agaataattg aaaatttcta ataaaacaat tatattgtgt gatagaataa
    12421 attacatttt tttcttaatc ataaattcat gataaattaa ttatgaattt tgaaattgat
    12481 tattacaatc attacaaata ttttcacggg agatatcata cagtatgaaa gggaaaataa
    12541 taacatctct attttttaca tttgtagcta taacttcgct gatttttttt attattgctg
    12601 tcattatccg gattatgacc tacccttttg ataaaaggtt gcgtttactt catcttttta
    12661 cctgtttctg gggttcactc tatacctatg ttatgccacc atggaaaatt actgtacagg
    12721 gaagaaataa gatcaaaaat agtaaaccgt atatcattat ttccaatcat cagtcacagc
    12781 ttgacatatt agttgcattc agattatata aacattttaa atgggtgtca aaagcagaaa
    12841 tatttaaggt tccggttata ggctggaata tggtattgaa ctgatatatc aaactggtgc
    12901 gaggcgataa aaaaagtata gcacagatga tggatgagtg tgagatgcat ttaaaagaag
    12961 gtagttcggt gtatatattc cccgaaggct cacgttcgcc ggatggtcat ttaaaacaat
    13021 ttaagttggg agcatttctg ttagcaaaaa agttgaatct tcccataatg cccattatca
    13081 tagaaggtac acgatttgcc cttcctaaat atagtatcga ctatcatgga aaacatccta
    13141 taatattacg ggtacttgat gaaataacac cggaaaaatt taaaaacatg agtgtagagg
    13201 agctgataga ttatacatgg aactatatga atgctgagtt aaaaaaattg aggattgaga
    13261 tgtatggcag tgattcaatc tgagaataat tatactttaa attagcttgc actattgatg
    13321 tatatcctgt agagtaagaa catataccat tattacagtg aggttgtatc aatgtctcaa
    13381 cctgatgatt ttaccttaga tgacgattta tttaaattgg atgatgagat agatatacag
    13441 gacaatatac ccgagaataa acctgaagca aacaacaata cggtgttgga ttcgggaaaa
    13501 gattatggcg atagttcccg taaacggtca tccagagatt tccagcctga catggatgca
    13561 ctgctcatca cagcacagtc ttcaatgatt attgaaggaa tgaaaaaata tactgaacga
    13621 gatttttctt caaataatct ccccatttat ttagaagcat taaacggcgt taatctttat
    13681 ataaaaatac tagaccgtaa tccaaaaaat tatcataaat taaaatcagt gataaatgct
    13741 gatcaggatt gtcaggaggt tgaaaagatt gcatttgatt tatacaaaaa aatgtttggt
    13801 actgtgcctg aatcagatca ggaaaaactg gttgcatttg aaaagttttc atcattattt
    13861 aaggatgcag tcaacagagc aactatcaat gcttccatga aagaaataaa aaaatacttt
    13921 ttattgtcag gggggcttga tgaaatcaaa gtttcaaagc ttatagccca aaacgatgaa
    13981 gagcttgcaa aagatatttc aaatttacag cgatatattg ccatgggatt ggcctatatt
    14041 gaaaaaggga atattgaaat aattaaagga ttaaaaggaa gagatctaaa caattatatt
    14101 atacgtggtt cccaactgtt gacattttac tttaaaaaaa tcaataatga ccgccttgcg
    14161 gattactatt cgaggatcaa ttctatttat agcaaatatt ttgttataaa agattaatac
    14221 tcccatcttt tttacttgat atagtgtaaa taatattaag cttagataag cagaacaacc
    14281 tttttttagt gcattgattt gccgacacat attactatgg caatacatat ttttaatttt
    14341 ttcaatcaat catcattcaa ttgctatgat acactgcttg ctaattcaag cagtattgta
    14401 tccagcatga acatcccctc atccgtgcat tgaatgcaga tatcaccatc acaggtttgc
    14461 atgattaata atgatttatc aattaaacga tgtattgact ggtaaaatga ttcagccggc
    14521 aggtaccctg ttttgttacg catgtcattg atcataaatc cctgttttaa cctgagctta
    14581 ttcatacaat attcaaggac gctgtccggt tgtgtgcgta tatcctccgt acggcttgca
    14641 attgcatcgt tcatgtagtc atcaatggta ccattgtaat agcgtttgtt gttataaaat
    14701 gaatgtgcac cgctaccaaa accaatatag ctttcaaaat tccagtattt tagattatgt
    14761 tttgactcgt atcccgggat tgaaaaattg gaaatttcat aatggttgta gcctgatgcc
    14821 accagcgcat cgattgtttt ttgaaacatg attcgctgaa aatttgcata ttgcagggga
    14881 ttttttgtgc gtaagcgcaa aggtgtattt gtttcatacg tcagcatata tgcagagata
    14941 tgtttgggat tacactgaat gatactgttg agcattgaac gatagttagt taccgactga
    15001 acgggaatgc cggcaataat atcaatgcat ggtataatgt caggaatagt gcaaaattcc
    15061 tgtatttctt ccatggtgca aagcattgac tttcttccaa tatacttatg tgctctggta
    15121 tcaagtgttt gtactcccaa tataaatcta ttaaagccta tgtgcgatag ttgttgtata
    15181 taatttttgg aatagtcatc gggattacat tccaaagtaa tctcaacctc agaggcaatt
    15241 gcataataat catgcagtac agtaagcaag acattgtaat cactgcattg cagcagcgac
    15301 ggagtacccc ctccaatata tatggtatct accagataat tgtttagttc agatgaataa
    15361 tgaatgattt cggataccag tgcattgatg tatgcatgtt tgatttgttg aaaattgcta
    15421 tgtgatggat tgcacggtat ggaatagaaa tcgcagtaat cacatttctt agtattgcag
    15481 aatggaatat gaatatatat gccagaatgt atcatttatc aatcattaaa cagggatgag
    15541 atgattttgg tgtaaattca ccactcaggc aaagcgccat ctggtgcaat gattcatgag
    15601 tatctgaaaa agacattaaa agaggatact cgccaaattc ttttagatag ggatagggat
    15661 tgccagttac caataaaagt gtattgcaat cgttttgttt tgcagtttta ataattgatt
    15721 gcaactgttt cgtagtataa catccactat caataaaaag aattttttta tttttcaata
    15781 actgtattga atgtattaca tctgcattta tagctttaaa aatatcctca acttccttat
    15841 ccgttgtaag gatagttgct ttcctgtttt cggctggtat aaatgtagtg aaatcatcgt
    15901 tttttgagtg atacactatt gactcccttg ataattcagt attcagtgta tcagcctgtt
    15961 gcatcatttc caggtaacta tcgcccccat taaaattact attatatatt ttattttttt
    16021 tgattgtcat gataccataa cgcattttaa cctctataat tttttttact gttgatacta
    16081 ttctgtcttt tgatacttca ttttttaaca ttgcatttga taacgaattg ataatatcct
    16141 gaacatgatc ttcataggag cttatcaaca caatatcagc ccctgcttta acgcttgcaa
    16201 cagcagcagt tccctgtgaa taataaccgc tgataccctt catctctaaa tcatcggtaa
    16261 tgattaaacc atcaaacttc attttttttc gtaataaatg atttaaaaaa tattcagaca
    16321 tggtggcagg ttttacaatg ccaagttggg gaaaagcaat atgagccgtc ataatacatt
    16381 ctactccgga agatattgct tttttgaaag ggacgaattc agttttattc aatgatgcat
    16441 gagttttatt tatgaccggc aatgttatgt gcgaatcctg ataggtatca ccatgccccg
    16501 gaaaatgttt ggctacagca atacatcgtg actgttgtaa tccttgaatg tatgcagctc
    16561 caagattagc acaaacttga ggtatgcagc taaaggaacg tatattgata acaggattat
    16621 tgaaattgtt attgacatca agtaccggag caaaattcat attgatgcct gctattctaa
    16681 gctgcatccc tacaatctgt gccatccggt acacttttga tggattatta atgacaccaa
    16741 gtgcaaaatt accgggaaac tgcgttacgc cattttgaat gcgctttacc ctgccccctt
    16801 cctggtcaat ggctataaac aaagggatac caatatgttc catagcaata ttttgaagat
    16861 cattgcagaa tgttttgcag gagccatcag taaggttata accaaagata ataacgcctc
    16921 ccggcttgta tttttttatt atagctattg tcttatcatc aacaacacct gatggcaaag
    16981 caatgataag catctgcccc agtaaaacat cattggctaa tccactggca atttttcctg
    17041 ctgtgatata atcttttggt tgtttacatc caattagttg aactaaaact aataagagag
    17101 aaacagcggt cattggttta atcttcatag caatccattt caagatattt caattatttt
    17161 tgattgtcgt tgcattgtta tagattatgg tgatttattg caatagtaat tttttttaga
    17221 tttaagtatt aaattcatat ataaaacttt attttgcctg tccttgtata ctcacaatag
    17281 caacaaagct gccttttttc aattggttat tagatgaaaa aaactatttt tttaattgat
    17341 ggtgttacca caacctgcat ttcaggagag catagagcat tatttttaca atacatgaaa
    17401 agcctgatat atcattacag atgtttgttg tctattgcag tgcgtttgct acaaaacatg
    17461 tacaaaggca tgaatttagc tttaaatgta tattgtatag aaaaataaac gggagaaagt
    17521 tattgtaaaa gttgatgata gatgttttaa taaagacttg catgatagca caatggtgaa
    17581 tagcactgga ctatcatagt gttctattga tgccatgcat tgtatagcga tatattcaat
    17641 atacagtggg ttgaaacgat aacgatctta aaataaaata cctttatagt agtatagttt
    17701 tttatatacg ataactatcg ctcaaaataa agaaattagc ttgcaaaata atgaattgca
    17761 ttatagtata tggattttaa taattgcaat taacgatgta ctgtatttcg tgttgatttg
    17821 gagattgtta taatgagagc tataatcaga ttacatcaac acttttgttt ttaaacatca
    17881 accatgtgct catgttttga tagaatatat gaatgataat acaataaaaa agtatatcga
    17941 tagctttagt gctgatacta tcaccgtacc aacagtcatn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnna aagtattcat ctaaagcgga aaaaggactc agcgtgcgag
    18121 taaatggcca gcagatttcc ttaaaaggaa ttggtgctgg tttaaagata atgtgtgctt
    18181 ttatggactc aattaccatc gatcttattg acaaagaaaa aatgatttct gatactgtca
    18241 gcaaagttac tgatgggact atattatcaa tgcacaggaa gaggagatac aaataatgag
    18301 accggaaaaa gcatataaaa atatggaatt cctcaattca cgagaagcac gcattatcag
    18361 gataatgtgc gaatatattg aacctgaaaa aaggtttaaa cattataaaa taaaacatac
    18421 cgttgttttt tttggttctg ctcgtaccca accggatgat cctttaacca tgcaatatta
    18481 tcatgatgcc tgcaaacttt catatttgct ggcacaatat ggaaaagaat tggaggcagt
    18541 tcatgacggt tttgttatat gtacaggcgg tggtcctggt ataatggaag cagcaaacaa
    18601 aggagctaat gaagctggag tatattcaat agggttgaat atatcactcc cttttgagca
    18661 aatacccaat caatatatat ctcaagaact aaatttcgaa tttcattact ttttcatgcg
    18721 aaaattatgg tttctttatc atgcaaaagc tattgtagta ttcccgggcg gttatggcac
    18781 acttgatgaa ctatttgaaa cattgactct ggttcaaact aataaattac aaaaacagga
    18841 cattccaata gttttatatg atgagaatta ttggaataag ttaattaatt ttcagatgtt
    18901 gattgattgt ggttatatag ataaaaaaga ttttgatcta atccattttt gtaattcccc
    18961 tgaagaaggt attgaatatt taaaaccacg tttaaaagga ttaatagaaa aaattaacca
    19021 ttcaattggt gagtaatgaa tcatggaaaa gattaaagag ctatctggtg gtcttgaaaa
    19081 tgtaaccata tttacatatg ctgcaataga atgtggcatt cggtatgaaa atagattgga
    19141 tttttcattg atatattctg ataaaccatg ttgggccgct ggtgcattta ctaccaataa
    19201 attttgcgct gcaccagtta aactgtgcag agaaagaata aaaaatacaa ttcatgcagt
    19261 tatcataaat gctactaatg ctaatgcggc aactggtaaa cagggctatg ataatgctaa
    19321 gctactaact gctgaagtag caaaaatttt atcaatagat gaaaagactg ttttaatggc
    19381 atcaacgggt attattggag tgcaattacc ggttgaaaaa atgaaaaata acctgcccat
    19441 attggtaaag aatttaaata gcgagtcagg aaaactgctt ccaactgcta taatgacaac
    19501 cgatactttc ccaaaacaat atgcattatc ctttgaaaca tcaacaggta tatatactat
    19561 tgcaggtatc gctaagggag cggggatgat agctccagac atggcaacgc ttttatgctt
    19621 tgttgtaaca gattttccat tacaacaaag gttattgcaa aaattgttta cctcaagcat
    19681 tgctgtttca ttgaatgcta taacaataga tggtgatatg tcaaccaacg atactgccat
    19741 acttttatct cctgcaatac atacaaaaca tacccatgca gatatagata tttttaataa
    19801 tgcactcact atgatgatga aacgattagc ataccttatt gtaagtgatg cagaaggagc
    19861 aacaaaatgt gttacaataa aagttgttgg tgcagcaaat gatagcgatg ctaaaattgc
    19921 agcaaaatcc attgccgagt cacttttagt taaaacggca atttttggca atgatcctaa
    19981 ttgggggagg attgcatgtg ccgctggata ttctggtgct gagttaaggg aggataaact
    20041 atcaatctat tttgaggata tatcgttatt caaaaatggc agtccagtca actttgataa
    20101 agacagattg attgatatat taaaaaggaa agaatataca attatcatag actttggtat
    20161 tgggaaatca ttgtttgaat atttaacctc tgatttaact tatgaatatg taaagattaa
    20221 cgctgaatat tctacctgat aatatataat ccttgacgca attgttataa actattacaa
    20281 ttatgagcaa tttatcaata ctatttaatt tttttcaaat gcggtatatt tcaaaaatta
    20341 tagatttatt tcaactaaac aacgttgtta acaggttaat aatggcagag tttaaaagaa
    20401 tagtatatag tattattatg ttttttattt ttatttcttt agtgaatggg caaactattg
    20461 ataaacataa gattattatt tattattttg aagatgaaag ttctttgcaa aattacagat
    20521 attactcata tattatacct gataatcttg cgatagaact tcaaaatcac gtggagtatg
    20581 aagtaacggt tttaccggta actttcaaat atatttccga tgatatgcct caggaagaaa
    20641 aagaaaatac tattacgatc ctttccacaa gagctaaaga ggttaatgcc aggtttgtta
    20701 tcattggttc ttataaagta gaaaaaaaaa gaatttttat acaaacacaa atttacaata
    20761 ctgtaaccgg tgaactaacc gatgctgccg aggtagaaga aactattggt gcaatagtat
    20821 atgaaattat tgaaaagata acattaaaaa ttaatagcca gttgcagaaa acattagatg
    20881 ttgaaaaaga aaagattgca ctttccccct ataaagattt ttattcaaca ttccaaaata
    20941 gttactttgg ttattcttat agttacgtct ttttcacggg aagctgggaa aacttatata
    21001 atgatccaaa aatgagtagc gtctatattg gtttccctct atcgaaaatg tatgcttcga
    21061 actcggtgtt tataaaaaat atttatattg ttcctacatg gcaattttat tcaactaaca
    21121 atcatgattt aaaaaatatg aatgattcct atctatcaat ttataccgga tctatcgcgt
    21181 tgcaatatca atattcatta tcccctttta tgattgtttt tgctagttta ggtgctggcg
    21241 tttcgtattc agatatgcgt tttgttcttg catcagaagg aggccctttc tctcctgaaa
    21301 ctaaaaccga tagtaccgat ccatattttg gtgcaaacct cggagcaata ttttatatac
    21361 ataatatagg tattagtggt ggacttggat ataaccgaat attctataaa ggagatgatt
    21421 tagcatcatt tgttatgtcc tttggcatct tatatagatt ataaaaaata tcacagtaac
    21481 tatcgcacat atagtttttc aatttttttt atttaatcag ttttttacta cagttatagt
    21541 gaaaagcaat gaaagaatta tcttattatt atgaaaggag atagtaccag tgaaatctaa
    21601 aattgtttat tctcataatg actactggtg taagcaccag atttatgatg caattgcgta
    21661 tggttgtgat atggtagaag ctgacgtaat ctatatgaac aataaaataa tactgtcaca
    21721 ctcatggcgc cctttttcat ttatgtgcta tggcaattta gaagataact atttaaagcc
    21781 cctttcacaa tactgcaagg ataatcctga taaaaccatg tggatgtatg ttgaatttaa
    21841 agactcaaac gaacatatta tcgaattatt gcataagtta tttaaacaat atcaaacaaa
    21901 gaatttgcga tatactgtat ccgcactggg tagtaaatgg tatcagaaga aaagatatga
    21961 aacagccatg caattttaca aaaaatataa agatgaattt tcacttttat ggaaagaaac
    22021 agaattggaa caacaatacc aggttatttc ggttgattta tatccacaat ccatatggca
    22081 tttttgattt cacagtatag aggaagtttg tctgttgttg ggctgcaatt accggaaaga
    22141 aatgttattg gaataaatgc cattatacta ttattcagag gcaaagccaa tagtcattgt
    22201 gaaacgatag tgtataagat gagtttagtc gatgcaaaaa atatccttta tgtcctttat
    22261 gaaagaagcg attctttggg actaccctat ctcaacatga cactatggga gatgtattgt
    22321 taagcacgtt gcttccttat catgatgact cttacgaggt tattcttaac atttatgcag
    22381 tctaaatttg gaatacaatg ccataataag tttgggatga cttttttatt gaatatgtgt
    22441 tctggatgta atcgtacaac aaaacgtaca tagtaaacta ataaatttaa ttttgaggaa
    22501 atgtaaaaat gaagttaatt gtaaagaaat ggtttattct ctcaattgct atttatttag
    22561 cttcagtgct gctaaatgga ttttcagcag aaacagctac agcaataatt attgcatcag
    22621 cattgctggg gattttcaat gtttttatta aacctgtatt gatctttata acattaccca
    22681 tcaatctttt aacacttggc ttatttactt ttgtgatcaa tgcattactt ttgtatatgg
    22741 tttcatcgat tgtaaaggga atttatcttg attcattctg gtcggcttta ctggcatcga
    22801 taatcataag tatactatcg atgataataa actggatatt tgccgataga taaagagaaa
    22861 caattactgt atagttatta aatctatgtt tatagtatca attggtgcag ggaaaaatca
    22921 attaccgtta attcgggcat gcaaggattt gcaatgtaag attgttggcg tggatataaa
    22981 tccccatgct gagggttttc cattatgtga ttttaaaatt attgaatcta tttataacta
    23041 tcacgaaatt taccaatcct tgaaagaatt gcttgttttt gatgacattg ctgccatcgt
    23101 tacccgatcg tatggcgaag cattacgaac agtatcgtat ctatgtcagc agtttaaaat
    23161 accacatatt aattacgcta tgattgatta ttgcattgat aaatctaaat ttagaactat
    23221 cgccaataaa accattacga tgcctcattg ttttactatg ggtaaaaatg ataaagggaa
    23281 catactaaca ttaagcgaca tcaccttccc ctgtattata aaaccgtcac aaggacatgc
    23341 caaacacaat gtgcaactta ttgacagtgt ccaacattta aaacaaagta tagcttcaat
    23401 gaagcacagt acaatcatgg ttgaagaata tattgaaggt gatgaaatta ttgtcatagg
    23461 atttgtcatc aataaaaaat tttatatttg cgacatcagt gataaggtac tgaatgccaa
    23521 accatatttt gttgatagaa tgcatctgtt gccatcaaca tattatcatt atcataatca
    23581 gctaaccagc attggacagc agatagctga tcttttttta ctggaatcaa ctccgttact
    23641 gatggaatgt gttattagca ataacaccat ctatgtgatt gaagcagcct gtgagtttgg
    23701 tggtgagtat ctggcagatt atgctatccc agcccgttt
//
LOCUS       DAOSOX010000016        22940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2265_length_22940_cov_8.200743, whole genome shotgun sequence.
ACCESSION   DAOSOX010000016 DAOSOX010000000
VERSION     DAOSOX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_22940_cov_8.200743"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLX22_02300"
     CDS             <1..185
                     /locus_tag="PLX22_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18765.1"
                     /translation="NEEIINASQLQKSDDIQQLSIEISKIQKQLSALYDKLEEIMSQH
                     EERTRHYERLMQEYSL"
     gene            complement(202..594)
                     /locus_tag="PLX22_02305"
     CDS             complement(202..594)
                     /locus_tag="PLX22_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18766.1"
                     /translation="MKDIIAKILIVDDEDVVLKSCLRILQKLDYEVDTAYSAQTALEY
                     LQKKKYDIVVTDLMMPGMDGLQLLNEIKKLYPDTVVVIFTGFATVETTRQALKAGAFD
                     YIPKPFTPDELRTVIENAVKYCKNSNQK"
     gene            complement(604..2556)
                     /locus_tag="PLX22_02310"
     CDS             complement(604..2556)
                     /locus_tag="PLX22_02310"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOT18767.1"
                     /translation="MNKRFASQIRFKLFISILSIVLLSGVLSTFIGIRTIQKHVVGQA
                     FDTVLKDLDTADFIYTSRVALKSKMLKHISLLEYIQIAILHNDRAVLRSKCKEIQQEL
                     GIDIVNITDAQGKIIVRANNDSYGDDVSDDNQVSYVLKNKIPCEGTDVVLYKHLEREG
                     ESIASKAIIKIIPTPKSRKVQRTIIKDALTISVAIPIFHNNNFIGIIYGIRILNNYNG
                     IVDQIKQLVFKEEKLGGLDVGTSTIFLDDVRVCTNVKKPDGTRAIGTLLSEEVYNKVI
                     LQGKLWWDKAFVVNNWYISAYKPLLNIYGKPVGILYVGILEKKFDEIKKSSTLNFAFT
                     TALIAIIAVGISLYLLNIIIRPVRTLVKASESVINGNYDTHIEIETEDEIGYLCYTFN
                     QMIDAIKERDKKLKEQTQDQILQSEKLASLGRLASGIAHEINNPLTAILTYSSLLLED
                     LKETDFEEDLRVIIKETLRCRDIVRNILDFARSTKPDIKRTNVNNLINEVLLILEKHV
                     NFHNIAINKYLDPDVPDMFIDENQFKSVINNLAMNAADAMPQGGTLTVQTKYNFGNDS
                     ISIIVSDTGVGIKEEHLDKIFDPFFTTKEQGKGTGLGLAVTYGIIKRYHGNINVHSEV
                     GKGTVFTITFPLLKNKEEYSEYDKSK"
     gene            complement(2841..3242)
                     /locus_tag="PLX22_02315"
     CDS             complement(2841..3242)
                     /locus_tag="PLX22_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18768.1"
                     /translation="MAKKILLVDDDRDLVESLKQALVMNGYEVEVAYSGAEGLKKLLS
                     YKPDCMILDVMMETDTAGFEISYQIRSNRETSKYKDVKNVPIIMLTAINQVTNSRFSL
                     NEDQSFLPGINDFLTKPVRIDELLDKIKKYA"
     gene            complement(3265..3795)
                     /locus_tag="PLX22_02320"
     CDS             complement(3265..3795)
                     /locus_tag="PLX22_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOT18769.1"
                     /translation="MFAKLSKKAFPKSLWVYHCNSGACNGCDIEILNILTPYYDVERF
                     GIKLVASPRHADVMLISGAVTRPTFPLVKKAYDAMPSPKIVFGIGSCATGGGCWFDTY
                     NVTGGADKAVPVNYFIPGCPPRPEAIIYGVALALGLVDKKAAPVELKQIEFPIDIYQR
                     TKAWEERNVIYQLLYD"
     gene            complement(3798..4343)
                     /locus_tag="PLX22_02325"
     CDS             complement(3798..4343)
                     /locus_tag="PLX22_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT18770.1"
                     /translation="MWLTSKIKQLFVVTKPGTVTLAYPFDPRPVPENFRGQPYWDHHK
                     CIGCGGCANHCPARTILVRDLCNEIRVMVYDGSRCTYCGRCADLCPEKAITMTPEYEL
                     ATNDKRDVNETMELFMLTCQRCGRCFDHEIKNAIDRLTYLGYRYDNLEVRAVIPVSTQ
                     VFDKDMYEKTMTYKRPGKIGE"
     gene            complement(4368..5267)
                     /locus_tag="PLX22_02330"
     CDS             complement(4368..5267)
                     /locus_tag="PLX22_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOT18771.1"
                     /translation="MDILLILLNIILFLGLAPLYEGIIRKLTAKIQSRQGPPIYQPYL
                     DIMKLLGKDNLVSGNWAFKLAPMMAFAAVTAVITIVPIGFKPNYLTHYADIITIVYLL
                     TLAGISILLGALSSKNAYAMIGASREMVTMIMVEPILAMTFILGAVKMKSLAIDASMF
                     SVLSNGYSWSVVLMLVVYVMALQAFVARQPFDISEAEVEILEGPFIEYSGPNYALFKY
                     SMMLKQMFYAVLFVTVFMPFLFTGIFIIDVLLQLIAVAVVFAVIALLGSTNPRLRIDQ
                     AVKYYAVLIGISLCTIAMSVYGI"
     gene            complement(5280..6953)
                     /locus_tag="PLX22_02335"
     CDS             complement(5280..6953)
                     /locus_tag="PLX22_02335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOT18772.1"
                     /translation="MPSIDQIKSELTAKFKGSLKGFDTPLPTRLFVDVDKNSIRSLCN
                     AVVGMGARYLVSIGYDNIARDNTLGLVHTFSFDNDNVFCCVRTKVPADNPVCDSITPD
                     IPNAGWSEREYMDLLGMKFEGHPKPKRLILADDWPAGIYPLRKEVPWNLMPPAAEDVA
                     YQLDEAPEGCTTVPIGPFHPTLHEPAHFAVYVDGETIKGCDYRGFMTHRGIEKLCTTQ
                     VTYNEVPFIAERICGICGSVHATSYSQAVELAAGITISRKAEYIRTFMLELERIHSHL
                     LWLGVAGHLIGFDTVFMHSWRVREPVMWLAEKITGNRKTYGMIIIGGVRRDILQHKDD
                     ILNVMNTVEKELIAIKNAIVGDTVIHRRTKGVGYVSTEDTVKWSLVGPVARARGVNID
                     TRRDHPYAAYDAVKFDVPVVDTCDVWGTLLVRILETFESIKIVRQVLNDMPVDEPLLY
                     EFDEPIKPLIHSIAAVEAPRGEVVHYLITGEENRADRWRVRAPTYPNLQGVPLMLLNN
                     QLADAPIIIGSIDPCFSCTDRMALVDLTTGKHEVLNGEQFEKLSRSKKG"
     gene            complement(6979..8841)
                     /locus_tag="PLX22_02340"
     CDS             complement(6979..8841)
                     /locus_tag="PLX22_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOT18773.1"
                     /translation="MNIDVYGILLQAMAVLLGAGLVVPLLANKRKLAGWINTIVIAIA
                     SSLLLYVAYNAIFVMESSETQAIYFGGVCVYFLIDAFSGFFIAIIAFMAVISALYSID
                     YMEHYKDYSLRPYFINFPLFILGMVCIVTVDDLSIGFTVAWQLMTIASYFLIRFEYKN
                     KENVRNANKYLILMELAWVFIVTGAFIINGSAAGDSIHTLTQKIGASTGIVPVIVYTL
                     LFLGFSFKAGVFPFGQLWLPDAHSIAPSPISALLSGVMIKTGIYGIIRTFFWMVPHDG
                     NTINPLVWGAIIATFGVVTLFIGTSQSLKQSDAKRLLAYSSIGQIGYIVLAIGAGLYM
                     YHSSNMFVKMLSFVAIIGILYHVLNHAIFKGLLFLTSGSILYETGTKDLNKLGGLMTF
                     MPISAVIAGIASLSIAGVPPFSGFASKWTIISATLLGGSQVFFLVLFGIMALFTSALT
                     LACYVKFFGMSFASTGVEWNGDHKPHEVSWKMLLPKCVLAILCILQGIIPFVYYDTFI
                     KIFKSSSGSIIQHAFAELSTQNYIFTNAYGVTVNVPGMHYANAVAVPLVMIVIIIIGF
                     AVAYMVRKSAGAQEKVMPTWLCGYQDLNNNNRYKDKHMFAALKSILWWTGGNVK"
     gene            complement(8831..9334)
                     /locus_tag="PLX22_02345"
     CDS             complement(8831..9334)
                     /locus_tag="PLX22_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT18774.1"
                     /translation="MSFTALQNIFNTTTCIVGLGNYYRHDDAVGLFILDRLKAIDSNP
                     GVLLFNVEDIIESYVFTIAESNCNNVLIIDAIVGEAEPGTIVFGKLHDIVETGLNIST
                     HKLALQMSCKIWEASQKDVYLLGIVINNDDYGKGLSPVIHDKAETIVNMLQASIMSNQ
                     KECVYEH"
     gene            complement(9331..10164)
                     /locus_tag="PLX22_02350"
     CDS             complement(9331..10164)
                     /locus_tag="PLX22_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOT18775.1"
                     /translation="MSEQKIVTVPEIATIEEIKDEIVDVKTFYLRFDNKEIDGNFKIK
                     SGQFIMCTVFGAGEFAVSLPPSPENDRFHITVRKIGKVTNALHDLEVGDKVGVRGPFG
                     NGFPFEEIKGKNVIYVAGGIGLIPLRSSIVHVLQHRNEFGRILLLYGSRSPQDLMYQY
                     MLEQWQKIEGFETFITVDNGTPEWKGNVGIITTLFNKVEIPVENTVAFVCGPPVMFNA
                     VIKELMQRGIKDDMIISTLERHMKCGVGKCQHCAIGRTLVCTDGPVYTYRQIKILGEQ
                     I"
     gene            complement(10174..11226)
                     /locus_tag="PLX22_02355"
     CDS             complement(10174..11226)
                     /locus_tag="PLX22_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT18776.1"
                     /translation="MYLIKRDDIKKIAHALEEKYTLYGPYLDEVYGQCIFDTVNGDTV
                     NVNAPIPTNPPKQVVFPHLEKIMQYKYDKKAKKAEIAMLFDDAPKALFGLRSCDLTGL
                     QCIDRFFLGQEFVDEVYRHHRSRMFIVSSTCVVPFKQCFCVCTDSGPAANEGYDINLT
                     DLGDSYLAEVGSEKGKAFIEAFHFPAATDEHAKLKQKLVEKSITMFEDIAVESKAWVS
                     RVMNRVTTGFIKEEVWEYIGNQCIECGACSYVCPTCTCFNINDTSTVEGDTYRLRTWD
                     SCSYEGYSRMAGGHNPRKPIEDRRNKRFFCKLSYSQSKKYLRPGCVGCGRCAWVCPGD
                     IGLPNVVTYIRREIHE"
     gene            complement(11228..13195)
                     /locus_tag="PLX22_02360"
     CDS             complement(11228..13195)
                     /locus_tag="PLX22_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT18777.1"
                     /translation="MSYLPGVCTFCGTGCGHYLKVDGASVNGVFPSRVHPVSKGRLCV
                     RGWNIHELLSTHDRITKPHVKEGEVSYRDAITKLVSALSSYKPEEIAFYVSPRSSNED
                     NFALMKLARAVFKTNHISIQSESGHRNSLDVLFKGTGCAGMLGSLEDIRKAEVIIVAG
                     MDITTQNPIVGSELHYAARNGSLLVTIDSRTTKIARLSKKFMNIKPGTMRQVFDAIAY
                     YIDSEKWGKTTIAKYDEYRRYLETVPFKTIEEVAGVSVSDIMFVAEKLAKAKSAAVFF
                     TSGISGLQYDTVASIYNCFAIADKIGREGCGVNPVTGINNLQGSFDMGCAPDLLTGFQ
                     SIDDTAAKKRFEQQWNTKLPELKGRSVEELLADSTSPLKALVIVDHDDGIIKFADRLK
                     SLPFVAYIGAFTNKVADFAHVVLPITTYIETDGTFTNAERRVQLSVKKCNPAFDVLPL
                     WKLASDIAAAAGKPFNYAHASDIMDEIASLTPCYEALSHKALAKKHGIQWPCTKKNPD
                     GTKYCATDKPAFVLPQKPYAMVQANESYPYLLMLGKAQHYWHQNTIMHKTFIPLREYN
                     ATLLLYPDGYVAIAPEDAKKLGVRDRWPVKVVAEKASMKAMVMISDDVAPGTAYAPYF
                     IRDSITSFLLAYSDVVSQGEEATIPVRIEKV"
     gene            complement(13446..13580)
                     /locus_tag="PLX22_02365"
     CDS             complement(13446..13580)
                     /locus_tag="PLX22_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18778.1"
                     /translation="MKIAKNGQITILQGICDILAADGIEFNITYNGVYVHPVKSVAGN
                     "
     gene            complement(13816..14505)
                     /locus_tag="PLX22_02370"
     CDS             complement(13816..14505)
                     /locus_tag="PLX22_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT18779.1"
                     /translation="MKYEILGKPDFPCVKINLNAGESIVAESGAMVAMSTNMEIKTES
                     RGGIMKSLKRSVLGGESFFINTFTASDKAGEILLTSATMGDLEVIELSGNDIILSTHA
                     FVCSSQSVSIDSTWGGFRAFFGGEGLFFLKASGSGTLFFSSFGAIHQIQLQGEYIVDT
                     GHIVAFDPSVSFTIEKVGGLKSLFLSGEGLVCRFSGSGRLWIQTRNQNSFAAWADQFR
                     RVESSDNSDTM"
     gene            complement(14515..15189)
                     /locus_tag="PLX22_02375"
     CDS             complement(14515..15189)
                     /locus_tag="PLX22_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT18780.1"
                     /translation="MNINIKYNPSYALAVVSLNADEQIKAEDGAMVSKSVNVSIETKK
                     ASSSGIMKSAKIAFFGGTSFWLNTFTAQGSRGEVTLAPTLPGDIKKLDIAGTVFVQSS
                     SFLAASPSLEINTKFQGLKGFFSGESLFFLQISGDGPLLIASYGGIEELTVSESLIVD
                     TGHIVAFQDSLTYRIEKLGNWKSFFLGGEGLVCHFTGSGKIWVQSRNVSSLTSWLREK
                     LPPKRE"
     gene            complement(15193..15855)
                     /locus_tag="PLX22_02380"
     CDS             complement(15193..15855)
                     /locus_tag="PLX22_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006883275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT18781.1"
                     /translation="MEYVFKHNPTYTLLALKLAPDEKVKAEAGAMVYMSPEISIETGF
                     GSGFLSAVARKFFGGESLFVNIFTASHGGGEIGIAPQYVGDIQHKKMLGEAIMLQSGS
                     YLCSSPELTVTPQFGGLRSLFGGEGLFLLRVSGNGDLFFSSYGSIIEIPVSGNYVVDT
                     GHIVAFEDSLSFAVKKVGGWKSTFFSGEGLVCEFAGSGKLWIQSRVPNSFINWITKLL
                     PR"
     gene            complement(15990..18839)
                     /locus_tag="PLX22_02385"
     CDS             complement(15990..18839)
                     /locus_tag="PLX22_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT18782.1"
                     /translation="MSTILVIDDKKDNLVSITALLKNLIADCMVITAEDGKEGIEKAK
                     QSNPDVIILDIKMPGMDGFDVCRILKDDPMTRNIPVIFLTAIHTDTHSKVKGLKLGAD
                     AFLTKPIDQAELIAQINAMMRIKSAEDMLRKEKDLLEDMVLQRTGELLASQMRYKSLF
                     NSISDMVIIHKSDGNILEVNTTTCNRLGFSHESLAMMTLQQLTSEGDSNRIHTFLQSI
                     SENTDWYETAFVDSHGNTIPVEIASTTIYYRDENAVLSVARDITERKQIEQVRTQLSI
                     LLDNSDDAIIGTTLDGTIASWNKAAEKIFGYAFDEAAGCHVSLLSPPYRPDEFEQLIE
                     RVKHNEGIAHYESEGVDRHGAIHNMSMTVTPLEDERGTIIGCSIIARDLTDVKRTKEK
                     LKQGLDIIQNLEDIAPAYFLTLSEEGTVITCNKAMLQATGYKASEVTGKSCCTLFIPD
                     FGQRAFRNQLSHMKKTKATHIFETSIVMKDATERIVQWHARPVYDADVFNYFMLIGID
                     MTEKKQLEKKILQANIDERNRIARDLHDGLGQHLAGIIFKTEMLRLKLKDEFSNEVPE
                     IVEIQSMVHQAIEHTRLIAQGLSPVDLNNKGLHVSLQEIIADIDKNFKMKAILEWDVT
                     SNIDALVDTVHVYYIVREAVNNAVKHGNPKHIAVSVCEDDNDYCIRIMDDGRGIPDDV
                     DVTKGMGMHIMQYRAWIINAALQIQNNKTGGTLVQCTIHKSLKPVTEKLKSTKKFSIV
                     VVDDHPIVRQGLQQLIASHKNLEVIGEARSADEAIDVINKRVPDCVLVDISLNGTSGI
                     ELVKALKQRFPKLPALVLSMYDEKLYAERAIKAGARGYLMKNEAPDKIINAIYTVLKG
                     DIYLSGEVKELIVNALSDRGVIDSSVEGLTNREFEIFQLIGHGLGNKHIAEKLHISVK
                     TVENVREKIKMKLHLDSSQDLLQYAIQWVREHGGD"
     gene            complement(18836..19891)
                     /locus_tag="PLX22_02390"
     CDS             complement(18836..19891)
                     /locus_tag="PLX22_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT18783.1"
                     /translation="MNKEFLHSISHELRSPLNSILTLSKVLLMQADGRLTPEEKEYLA
                     IIERNGSRLLALVNDLIEVFEIDSGIELHITDVMVSTILSDILERYEGILEKKGLLLH
                     THLDDVILQSDETRVYQVFEHLIDNAVKFTEEGCITVSAHAHDGMCTVSIADTGIGIR
                     SEFLDTLFTGFTKASGSLSSKYPGTGLGLTIVLKLLSILGGTIHVESQLGKGSCFTVQ
                     LPLQVEAKPQADRKKILVIDDDSDSVVVVKALLSKLYDITETQSGTRGIALAHEVKPD
                     CILLDLSLPDLHGLEVVKELKSDDHTKHIPVIIVTASSLKNERSVIMASGCDDFIQKP
                     FPIEEFVLKVQHWVSQS"
     assembly_gap    20062..20161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20218..21189)
                     /locus_tag="PLX22_02395"
     CDS             complement(20218..21189)
                     /locus_tag="PLX22_02395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT18784.1"
                     /translation="MKMQVTAIIMVCCAAISCARDITKVTIAKKPLPDNFTVVMPEKV
                     AVSNDAVTCTIYAKKFAQGNVAYCEIIPAKDVQLVSVKFIDKEIVLSKKTWGYRGFFA
                     IDPELKPGVYHCIIYGDSFHIEIPVTIKRSFFPVSVIPMEFDKYSQKDYIETPEVKQF
                     IAECTKKKQKAFATRFDDCIQSIPYHPRNTHTINSAFWAKRRYLLYDSSTKKRQTVTR
                     IHRGIDLNGNSGDPVYAMLDGIVVLADKLFYEGNMVIVNHGNGIFSYYMHMEKLMVSS
                     GDRVVAGQLIGAVGSTGMSTGPHLHVSLMVHGVQANPLSLLVLPIRE"
     gene            21388..21741
                     /locus_tag="PLX22_02400"
     CDS             21388..21741
                     /locus_tag="PLX22_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18785.1"
                     /translation="MSIQISFSKLEKEFMPQFRERITTSEDIVDVQKIFSYTIKEMLQ
                     KAFEKEGITINEENIALTSSAPYYTITINNHSINELWNTSDLKDILQRFAEVAHKRYI
                     HLKKNMAKTKMKIRR"
     gene            complement(21751..22122)
                     /locus_tag="PLX22_02405"
     CDS             complement(21751..22122)
                     /locus_tag="PLX22_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOT18786.1"
                     /translation="MYIFELIAEQRIRDAMERGEFDNLPGMGKPLPRDELDTIPPDIR
                     MAYKIMKNAGIIPEEVELRKDIYSLQQLINACCDDDKRLEGLVKQLNAKQLRYTILME
                     QKGIHVPDYEYRKKVMEKLAK"
     gene            complement(22109..22420)
                     /locus_tag="PLX22_02410"
     CDS             complement(22109..22420)
                     /locus_tag="PLX22_02410"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT18787.1"
                     /translation="MVLKEHVISQLQHNFLSKGVDRVILFGPFARNTQTGKSDVDLIV
                     IKQTDKRFFDRYDDFNDIESYVCVPVDCLIYTPAEWEIIYNRIFFKNVLKKATEIHVH
                     L"
     gene            22539..>22940
                     /locus_tag="PLX22_02415"
     CDS             22539..>22940
                     /locus_tag="PLX22_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18788.1"
                     /translation="MKPLLYIVPILMLSHLAFANEYWQRQIESLQKEQKQVQAQPYTS
                     FVLEYIDARLAMAQELSALYHESLNNKHSEHATTTHYVNVLSRCFDDAAIDITRQQVV
                     QQLQLLQTSDAIDCTTNDYYIIHTELVLASVT"
BASE COUNT         6779 a         5265 c         3929 g         6867 t
ORIGIN      
        1 ttaacgaaga aattatcaat gcatcacagt tacaaaaaag tgatgacatc cagcaacttt
       61 ccatagagat cagcaagatt caaaaacaac tatctgcttt atatgataag cttgaagaaa
      121 ttatgagcca gcatgaggaa agaacacgcc attacgaacg actgatgcaa gaatattcat
      181 tataataaac taaaaaacca gctacttttg attgctgttt ttacaatact taactgcatt
      241 ttcaataact gtgcgcaact catcaggggt aaatggcttt ggaatatagt caaatgcgcc
      301 agccttcaac gcctgcctgg ttgtttctac tgttgcaaac cctgtaaaga tgacaacaac
      361 agtatcagga tagagctttt ttatttcgtt caatagctgc aatccatcca taccaggcat
      421 cattaaatca gtaaccacaa tatcatactt cttcttttgt aaatattcca gtgctgtttg
      481 agctgaataa gcagtgtcaa cctcataatc taacttttgt aaaattctga ggcagctttt
      541 caacactaca tcttcatcat ctactatcaa tatttttgct ataatgtctt tcatagttag
      601 tacctatttt gatttatcat attctgagta ttcttcctta ttttttaaca atgggaaggt
      661 tatggtaaat actgtcccct ttcccacttc gctgtgaaca ttgatattgc cgtggtaccg
      721 cttgataata ccataggtaa cagccaatcc taaacctgtt cctttgccct gttccttcgt
      781 tgtaaagaag ggatcaaata tcttgtcaag atgctcctct ttgataccaa caccagtatc
      841 agaaacgatg atagatatgc tgtcattccc aaaattatat ttggtttgaa cggttaacgt
      901 accaccctgt ggcatagcat cagcagcatt catagctaaa ttattgatca ctgatttaaa
      961 ttgattttca tcaataaaca tatccgggac atcaggatca agatacttgt tgatagcaat
     1021 attatggaaa ttaacatgtt tttcaagtat taacaaaacc tcatttatga ggttattaac
     1081 attggtacgt ttgatatcag gtttggtgga acgggcaaaa tccagaatat tgcgcactat
     1141 atctctacat cgcaaggttt ccttgatgat tacgcgcaaa tcctcttcaa aatcagtctc
     1201 tttaagatcc tccagaagca gtgaactgta ggttaaaatt gctgtgagcg gattatttat
     1261 ctcatgagct atccccgaag ccaaccgccc cagcgatgcc agcttttcag attgtaaaat
     1321 ctggtcctgt gtttgttctt tcaacttttt gtcgcgctct tttatggcat caatcatctg
     1381 attaaatgta taacacaggt atccaatttc atcctctgtt tcaatttcaa tatgtgtatc
     1441 ataattccca ttgatgaccg actcagaagc tttaaccagt gtcctcactg gccgaataat
     1501 tatatttaat agatacagtg aaatgccaac agcaatgatt gcaatcagcg ctgttgtaaa
     1561 tgcaaaattc agagtagaac ttttttttat ctcatcaaat ttcttttcca gaatgcctac
     1621 ataaagaata cctaccggtt tgccatatat attcaaaaga ggtttgtatg cagagatata
     1681 ccagttgttc acaacaaatg ctttatccca ccacagctta ccctgcaata ttactttatt
     1741 gtatacctcc tctgataata atgttccgat tgcccttgtc ccatcaggtt tttttacatt
     1801 ggtacaaacc cgtacatcat ccagaaatat tgttgatgtg ccaacatcta aaccaccaag
     1861 cttttcctct ttgaatacaa gctgtttaat ctgatcaact atcccattat agttgttcag
     1921 tattctgatg ccatatataa ttccaataaa gttattgtta tgaaaaattg gtattgcaac
     1981 actaattgtc agagcatctt ttatgattgt tcgctgcacc tttcgtgatt ttggtgttgg
     2041 tataattttt ataatagcct tgcttgcaat ggattcgcct tcccgttcaa gatgcttata
     2101 taatactaca tctgtacctt cacatgggat tttattcttc aacacatacg aaacttgatt
     2161 atcatcagat acatcatctc cataactatc gttgtttgct cttactataa ttttaccctg
     2221 tgcgtcggtg atattgacaa tatctattcc caattcttgc tgaatttcct tacatttact
     2281 cctcaaaact gctctgtcat tgtgcaggat agctatctga atatattcca gcaaagaaat
     2341 atgctttaac atcttgcttt taagagctac tctgctggta taaataaaat ctgctgtatc
     2401 cagatccttc aaaactgtgt caaatgcctg cccaacaaca tgcttttgaa tagttcgtat
     2461 acctataaac gtcgatagta cacccgagag cagcactatt gacaatatac taatgaacag
     2521 cttaaagcgt atctgtgatg caaatcgttt attcattgca ggactattat ttattattta
     2581 ctattattga ataccataaa ttatattgca tttttatgca atcctttttc attgttacac
     2641 aattactaac ggatattgga aaatgttaat gttcataccc ccgcggccac taataaatat
     2701 tggttccatt gactgttaac aatacccata agaaacaaaa ctattcgcat acagaaaaat
     2761 aaccggaaca tcaaatatga tattccggct ttgcgattgt aaacaggttg atttgatact
     2821 atatgcacat cactgtaaac tcatgcatat ttcttaattt tatctaaaag ctcatcaatg
     2881 cgaacaggtt ttgtaagaaa atcatttatc ccgggtagga acgattgatc ttcatttaac
     2941 gaaaatcgag aattggtaac ctgattgata gcagtaagca tgatgatagg tacattttta
     3001 acatctttat atttagaagt ttcccggtta ctcctgatct gatatgatat ctcaaaacca
     3061 gcagtgtcag tttccatcat gacgtccaga atcatacaat caggtttgta cgatagtaac
     3121 ttttttaacc cttctgcacc actgtatgca acctccactt cataaccatt catcaccaga
     3181 gcctgcttca agctttcaac cagatccctg tcatcatcaa ctaataatat tttcttagcc
     3241 attgtgtgca ctccatttca aattttagtc atagagtaat tgataaataa catttctttc
     3301 ttcccatgct ttggtccgtt gatatatatc gataggaaat tctatctgtt tcaactcaac
     3361 aggtgcagcc tttttatcca caagacccag tgccagcgca actccataaa taattgcttc
     3421 tggccgggga ggacatccgg gaataaaata atttactggt actgctttat cagcaccccc
     3481 cgttacatta taggtatcga accagcatcc accacctgta gcacatgagc caatcccaaa
     3541 aacaatcttt ggagatggca ttgcatcata agcttttttt acaagcggaa aggttggacg
     3601 tgtaacagct cctgatataa gcattacatc agcatgccgt ggggaagcaa ccagtttgat
     3661 gccaaaacgt tcaacatcat aatatggcgt cagtatattc aatatctcaa tatcacaccc
     3721 attacatgca ccggaattgc agtgatacac ccataatgat tttgggaatg ccttttttga
     3781 taatttagca aacataacta ttctcctatc ttcccgggtc ttttataggt catggttttt
     3841 tcgtacatat ctttatcaaa tacttgagtt gatacaggga taactgctct cacctcaagg
     3901 ttatcatatc tatatcccag atatgtcagc ctatcaattg cattttttat ttcatgatca
     3961 aagcatctgc cacatcgctg gcaggtaagc ataaaaagtt ccatcgtttc attaacatcc
     4021 cgtttatcat tggttgctaa ttcatactcc ggggtcatcg ttatagcttt ttcaggacag
     4081 aggtctgcgc aacgtccgca gtaagtgcat cgagaaccat catacaccat taccctgatc
     4141 tcattacata agtcgcgcac caatattgta cgcgctgggc aatgattggc gcatccacca
     4201 catccaatac atttatggtg atcccaatat ggctgacccc taaaattttc cggaacaggc
     4261 cgtggatcaa atggataggc taatgttacc gtccctggtt ttgtcaccac aaaaagctgt
     4321 tttattttac ttgtaagcca cacgatcgta ctccttatgt tttagaatta tattccatac
     4381 acactcatcg ctatggtaca cagtgaaata ccaatgagca ctgcgtaata tttcaccgcc
     4441 tgatcaattc gcaaccgcgg gttggtagac cctaacaatg ctatgactgc aaacacaact
     4501 gcaactgcta taagctgcaa taatacatcg atgataaata taccggtaaa caaaaacggc
     4561 ataaacactg tcacaaacag tactgcatag aacatctgct ttagcatcat ggaatattta
     4621 aatagagcat agttgggacc actgtattca ataaatggcc cttccagaat ctctacctca
     4681 gcttcagaga tatcaaatgg ctgtcgtgca acaaaagcct gtaatgccat tacatacact
     4741 acaagcataa gcaccaccga ccacgaatac ccatttgaaa gcacactgaa catacttgca
     4801 tctatagcaa ggcttttcat cttaaccgca cccaaaataa atgtcattgc taagattggt
     4861 tctaccatta tcatggttac catctcacgg cttgccccaa tcatcgcata ggcatttttg
     4921 cttgaaagcg ctcccaataa tatagaaatc cctgctaacg taagcaaata cacaatggtg
     4981 attatatccg cataatgggt aagatagtta ggtttaaatc ctatgggaac tatggttatt
     5041 accgcagtaa cggctgcaaa tgccatcatt ggcgccagct taaatgccca gttgccacta
     5101 acaaggttat ccttccccaa tagcttcata atatctaaat atggctgata gataggtggc
     5161 ccctgccggg actgtatctt tgccgtcaac tttcgtatta ttccttcata caatggtgcc
     5221 agccctaaaa acaatataat gttaagcagt attaatagaa tatccatata acacctacct
     5281 tacccttttt tacttcgtga tagtttttca aactgttcac cgttcaacac ctcatgtttc
     5341 cctgttgtaa gatcaacaag tgccatcctg tcagtacatg agaaacatgg atctatactg
     5401 ccaatgataa tgggtgcatc ggctaattga ttgttcagca acatgagcgg aacaccctgc
     5461 aaattagggt acgtgggggc acgtacccgc catctgtcgg ctctgttttc ttcaccggtg
     5521 atgagatagt gtaccacctc accgcgtggt gcttcaaccg cagctatgga atggattagt
     5581 ggctttattg gctcatcaaa ttcatataat aatggttcgt caacaggcat atcgttcagt
     5641 acctgccgta ctatcttaat tgactcaaag gtttccagaa tacgaacaag cagcgttccc
     5701 catacatcac aggtatcaac cacgggcaca tcaaacttca ccgcatcata cgctgcgtag
     5761 gggtgatcac gcctggtatc gatattaact cctcgtgcac gagcaacagg accaaccaga
     5821 ctccatttga ctgtatcctc tgtcgaaacg tatccaacgc cttttgtcct tcggtggatg
     5881 accgtatcac caacaattgc attctttatt gcaataagtt ccttctctac cgtattcatt
     5941 acatttaaaa tatcatcttt atgctgaagt atatcccttc gcacaccacc aatgatgatc
     6001 atcccgtagg ttttacgatt tccggtaatc ttttctgcaa gccacattac cggttcacga
     6061 acgcgccacg aatgcataaa cacggtatcg aatcctatta agtgtcctgc aacaccaagc
     6121 cacaacaggt gtgaatgtat tctttcaagc tccagcatga atgtgcgtat atattccgcc
     6181 ttgcgcgata ttgttatgcc tgctgcaagc tcaacagcct gagaatacga tgttgcatga
     6241 actgaaccgc aaatcccgca tatacgctcg gcaataaatg gcacctcatt ataggttacc
     6301 tgtgttgtac aaagtttttc aataccacgg tgtgtcataa aaccgcgata atcacatccc
     6361 tttattgttt caccatcaac atatactgca aaatgagcag gttcatgtaa ggttggatgg
     6421 aatggcccaa tgggtaccgt tgtacatcct tccggtgcct catctagctg gtaggcaaca
     6481 tcttcagctg ctggaggcat caaattccat ggcacctcct ttcgcagggg ataaatacca
     6541 gctggccagt catcagcaag gataagccgt ttgggttttg gatgtccctc aaatttcata
     6601 ccaaggaggt ccatatattc tcgctctgac caccctgcgt ttggaatatc aggggttata
     6661 gaatcacata caggattatc agcaggcacc tttgtgcgaa cacagcaaaa aacattatcg
     6721 ttatcaaagc taaaggtatg taccaatccc aatgtattat cacgtgctat attgtcatat
     6781 ccaatgctta ccagatatcg agctcccata ccaacaaccg cattacacag agagcgtata
     6841 ctgtttttat ccacatcaac aaacaagcgt gttggcagtg gcgtgtcaaa acccttcaat
     6901 gagcccttaa actttgccgt taattcactt tttatttgat ctatcgatgg catattctac
     6961 ctcttactta atgttgtgtt atttaacatt accacctgtc caccacagga tggactttaa
     7021 tgctgcaaac atgtgtttat ccttatatct gttattatta ttaagatcct gatagccgca
     7081 tagccatgtt ggcattacct tctcctgtgc acctgcactc ttcctcacca tgtatgccac
     7141 ggcaaaccct atgattataa taactatcat cacaagagga acggcaacag catttgcata
     7201 atgcataccc gggacattaa cggtaacacc ataggcattt gtgaaaatat agttctgtgt
     7261 ggatagctca gcaaatgcat gctgaataat tgaacccgat gaggatttaa atattttaat
     7321 aaaagtatca taataaacaa acggtattat accctgaagt atacacaaaa ttgccagtac
     7381 acatttgggc agcaacatct tccatgaaac ttcatgaggt ttatggtcac cattccattc
     7441 aacaccggtg gaggcaaacg acatcccaaa gaatttaaca taacaggcca gggttaacgc
     7501 actggtaaaa agagccatga tgccaaacaa tacaaggaag aaaacctgac tgccccccag
     7561 cagagttgcc gaaattatgg tccatttgct tgcaaatccg ctaaaggggg gaactcctgc
     7621 tattgacaac gatgcaattc cggctatgac agcgctaatt ggcatgaatg tcataagtcc
     7681 gcccaattta tttaagtctt ttgttcctgt ctcgtacagt atgctaccgc tggtaaggaa
     7741 caacaatccc ttaaatattg catggttaag aacatgatac agaatgccaa tgatagcaac
     7801 aaatgaaagc atctttacaa acatattgct gctgtgatac atgtataaac ctgctcctat
     7861 agccagaacg atgtatccta tctgcccaat ggagctatag gcaagcaggc gttttgcatc
     7921 gctttgcttt aacgattgcg aggtcccaat aaataaggtt accacaccaa atgttgcaat
     7981 aatagcgccc catacaagag gattgatagt attaccatca tgaggtacca tccagaaaaa
     8041 tgttcgtata attccatata tccctgtttt aatcattaca ccacttaaca aggcgctgat
     8101 tggggaagga gcaattgagt gagcatcagg cagccacaac tgaccgaagg gaaaaacccc
     8161 ggctttaaag ctaaatccaa gaaataacaa tgtatagact attaccggca caatacccgt
     8221 tgaagcgcca atcttctgag tcaatgtgtg tattgaatcg cctgctgcag aaccattgat
     8281 gataaatgca ccggtaacaa taaacaccca tgccagctcc atcagtataa ggtatttatt
     8341 ggcgttgcgt acattttctt tgtttttgta ttcaaacctg atcaaaaaat atgaggcaat
     8401 cgtcatgagc tgccatgcaa cggtaaaccc aatgctgaga tcatcaacag taacgatgca
     8461 caccattcct aaaataaata agggaaagtt tataaagtag gggcgtaatg aatagtcttt
     8521 gtaatgctcc atatagtcaa tggagtacag tgcacttatt actgccataa aggcgataat
     8581 ggcaataaaa aatcctgaaa aagcatctat taaaaaatag acacagacac cgccaaaata
     8641 tattgcctgt gtttcagagg attccataac aaaaattgca ttatatgcta catagagcaa
     8701 caaactgctt gcaattgcta taacaattgt atttatccag cctgccagct ttcttttatt
     8761 agctaaaagc ggcaccacta aacctgcacc taataaaaca gccattgcct gtaataaaat
     8821 accatacaca tcaatgttca tacacacact ccttttggtt gctcattata gatgcttgca
     8881 acatattcac tattgtttct gctttatcat gaataacggg cgatagtccc tttccataat
     8941 catcattgtt tataactatg cccagtaaat agacatcctt ctgtgatgct tcccatattt
     9001 tgcatgacat ctgtaatgct aatttatgcg ttgatatatt caatcccgtc tccacaatgt
     9061 catggagttt accaaaaacg atggtaccgg gttctgcttc cccaacgatc gcatcaatga
     9121 ttaacacatt gttgcaattt gactcagcta tggtaaaaac atagctttca ataatatcct
     9181 ctacattaaa cagcagaaca ccaggattac tatctattgc ttttaaccta tcaagaataa
     9241 aaagaccaac agcatcatca tggcgatagt aattccctaa tcctacaatg catgttgtag
     9301 tgttaaagat gttttgtagt gcagtaaaac tcatatctgc tcacccaata tcttaatctg
     9361 tcggtaggta taaacagggc catcagtgca taccagggtc ctcccgatag cacagtgctg
     9421 acactttccc acgccgcatt tcatgtgcct ctctaatgtt gagattatca tatcatcctt
     9481 aatgccgcgc tgcatgagct ctttaataac tgcattaaac ataacaggag gtccgcatac
     9541 aaatgctacc gtattctcaa cgggtatctc caccttatta aacagtgttg ttataatacc
     9601 cacattgcct ttccattcgg gtgtaccatt atctacggta ataaatgttt caaagccttc
     9661 tatcttctgc cattgctcca gcatatactg atacatcaaa tcctgcggcg aacgcgagcc
     9721 atataaaagc aaaatgcgcc cgaactcatt tctgtgctgc aatacatgaa caatggaaga
     9781 acgcagtgga ataagcccaa taccaccagc aacatagata acattcttgc cttttatttc
     9841 ttcaaagggg aacccattac caaaaggtcc acgaacacca accttatctc caacctccag
     9901 atcatggagg gcattggtta ccttgccaat ctttcgcacg gttatatgaa atctgtcgtt
     9961 ctcagggctt ggcggtagag aaacagcaaa ctcgccggcg ccaaataccg tgcacatgat
    10021 aaattgtccc gacttaatct taaaattgcc atcaatttct ttattatcaa acctgagata
    10081 aaatgttttc acgtcgacta tctcatcttt aatttcttca atggtggcaa tttccggtac
    10141 tgttacaatc ttctgttcgc tcataactat cacctattca tgaatttctc ttcggatata
    10201 tgtaacaaca ttaggcagcc caatatcacc gggacatacc catgcacacc gcccgcaacc
    10261 aacacatccc gggcgtagat atttctttga ctgggaatat gacagtttgc agaagaatcg
    10321 cttgtttctg cggtcttcta ttggtttccg tggattgtgt cctccagcca ttcgcgaata
    10381 tccttcatac gaacatgaat cccatgtgcg cagccggtaa gtatcccctt caactgtgct
    10441 ggtatcattt atattaaaac aggtacatgt tggacaaaca tacgaacatg ccccgcattc
    10501 tatacactgg ttaccaatat attcccatac ctcttctttt ataaatcctg ttgttaccct
    10561 gttcatcact ctggacaccc atgcctttga ctcaactgca atatcctcaa acatcgtgat
    10621 ggacttctca acaagcttct gcttcagttt ggcatgctca tcagttgccg ccggaaaatg
    10681 gaaagcttca ataaatgcct ttcctttttc actgccaacc tctgccaggt aactatcgcc
    10741 cagatccgta agatttatat catacccttc attagcagca ggaccgctgt cagtacatac
    10801 acaaaaacac tgcttaaacg gtacaacaca ggtactggaa acaataaaca tgcgactgcg
    10861 atgatgacga tatacctcat caacaaattc ctgccccaga aaaaacctgt caatgcactg
    10921 caatcccgtt agatcacatg aacgcaatcc aaataacgcc ttgggcgcat catcaaagag
    10981 catggcaatc tcagctttct ttgccttttt atcgtatttg tactgcatga tcttttctaa
    11041 atgcgggaat acaacctgct tgggtggatt ggttgggata ggagcattaa catttaccgt
    11101 atccccgttg actgtatcaa agatacactg tccatacact tcatcaagat atggaccata
    11161 taacgtatac ttttcttcta aagcatgagc aattttctta atatcgtcac gtttaatcag
    11221 atacatatca caccttctca attcgtactg ggattgttgc ttcttcgccc tgtgacacaa
    11281 cgtcactata tgctaataaa aatgatgtta tggaatccct tataaaatag ggagcatagg
    11341 ctgtgcccgg tgcaacatca tcggatatca taaccatcgc tttcatactt gccttttcgg
    11401 caaccacctt caccggccat ctatcccgca cgccaagctt ctttgcatcc tcaggagcta
    11461 tcgccacata gccgtcgggg tataaaagca gggttgcatt gtactcacgt aacggaataa
    11521 atgtcttatg cattatagta ttctggtgcc agtaatgttg tgctttgccc agcatgagaa
    11581 gatacgggta actttcattt gcctgcacca tggcgtatgg cttttgagga agaacaaacg
    11641 ctggtttgtc tgttgcacaa tattttgtcc cgtcagggtt tttctttgta cagggccatt
    11701 gtatgccgtg tttttttgcc aatgctttat ggcttaaggc ttcatagcat ggggtaagcg
    11761 atgcaatctc atccatgatg tctgaagcat gagcatagtt gaaaggtttc cccgctgcag
    11821 cggcaatatc acttgccagc ttccacagtg gtaaaacatc aaatgcagga ttgcattttt
    11881 ttaccgaaag ctgtacacgg cgctctgcat tggtaaatgt gccatcggtt tcaatatatg
    11941 tagtaatggg taaaacaacg tgagcaaaat cagctacctt attggtaaat gctcctatat
    12001 aggcaacaaa gggtaatgat tttaatctat cggcaaattt tataataccg tcatcatgat
    12061 caacaataac gagagcttta agcggggatg tgctgtcggc aagtaattct tcaacgctcc
    12121 ttcctttcaa ttcaggaagt ttagtattcc attgttgctc aaatcgtttt tttgccgcag
    12181 tatcatctat gctctggaaa ccggtaagta aatcaggagc gcaccccata tcaaagctgc
    12241 cctgaagatt attgataccg gtaaccggat ttactccgca accttccctg ccaattttat
    12301 cggcaatggc aaaacagtta tagatagaag ccaccgtgtc atactgtaat ccggatatac
    12361 ccgaggtaaa aaaaacagcg gcgctttttg cttttgctaa cttttcagca acaaacatga
    12421 tatcacttac actaacgccg gcaacctctt caatagtttt aaatgggaca gtctctaagt
    12481 atctcctgta ttcatcatac ttcgcaatag ttgttttccc ccacttttca ctgtcaatgt
    12541 aatatgctat tgcatcaaac acctggcgca tcgttccggg ttttatattc ataaacttct
    12601 ttgatagcct ggcaatcttt gttgttcggc tgtcgatagt taccagcaat gagccgttcc
    12661 gcgcagcata gtgcaattcg ctgccaacga tagggttctg tgttgttatg tccatgccag
    12721 caacgatgat aacctctgcc ttgcgaatgt cctcaagcga gcccagcatc ccagcacaac
    12781 cagttccttt aaataaaaca tcaaggctgt tacgatgccc tgattcagac tgaatactta
    12841 tatggttggt tttgaatact gcacgggcta acttcattaa tgcaaaatta tcctcattgg
    12901 atgatcgcgg cgaaacataa aaagcaatct cttccggctt ataagaggat aatgctgaaa
    12961 caagtttagt aatagcatcc ctgtacgaca cctcgccttc ttttacatgc ggttttgtaa
    13021 tcctgtcatg agtggataat agctcatgaa tattccagcc ccgtacacat aatctgcctt
    13081 tgctgacggg gtgtactcgt gatgggaaaa ccccattgac tgatgcgcca tctactttga
    13141 ggtagtgacc gcatcctgtt ccacaaaagg tgcacacacc tggcaaataa ctcatagatt
    13201 atcctcgcta caattgtgag attgaaaaat tatcaaaaca ttcacttcca gtcttttaac
    13261 tggatacaat atggcacaat caataaccat tgtaaatagg ggattttgtc ttttttttaa
    13321 taggggaatt tgcttatgcg tatggaatat atatgtttat tttaatttta gtaatttttt
    13381 acaacatctt cccgaacttt atctcgtata gaatcaagct cttcataatg ctttgcgcag
    13441 tgttttcaat ttcctgcaac actctttacc gggtgaacgt atactccatt gtatgtaatg
    13501 ttaaattcaa taccgtcggc agcaagaata tcacatatcc cttggagaat ggtaatctgc
    13561 ccattttttg ctattttcac tgtacttttc atcccttatt actttttatt cattcttttt
    13621 ttttgttact accacgttgt catatacact tatcaaaaca gcaatactac aatatccagc
    13681 tttccttcta cccggatgag catataaaag ttaaagttat gactaatttc aatctacctt
    13741 ccatgcattt acttacatgt aaatatcagg aaacatcata tcaatgaaat aacaataatt
    13801 tcaatgcata atatgtcaca tggtatcgct attgtcagac gattctactc tcctaaattg
    13861 gtcggcccat gcagcaaatg aattttgatt acgtgtctgt atccataatc ttcccgagcc
    13921 actaaatcta cataccagtc cttcacctga gaggaaaagt gactttagcc cacccacctt
    13981 ctcaattgta aagctgactg atgggtcaaa agcaacgata tgacctgtat caacgatgta
    14041 ttctccctgc aattgaatct gatggatggc gccaaagctt gaaaagaaaa gagtaccact
    14101 gcctgatgct ttaagaaaaa agagcccttc accaccaaaa aaagccctga aacctcccca
    14161 tgtactatcg attgaaacgc tctgacttga acatacaaat gcatgggttg ataatataat
    14221 atcgtttccc gaaagctcta tcacctcaag atcacccata gttgccgaag tcaataatat
    14281 ttcacccgct ttatcagatg cagtaaaagt gttaataaaa aacgattcac cgccaagaac
    14341 agagcgctta agcgatttca taattccacc acgagattca gttttaatct ccatgttagt
    14401 agacattgcc accattgcac ccgattcagc tactatggat tcacctgcat taagattaat
    14461 cttcacacat ggaaaatcag gcttaccaag tatttcgtat ttcattatta cctcctactc
    14521 tctttttggc ggaagttttt cacgcaacca tgaagtgaga ctgctaacat ttcgcgattg
    14581 tacccagatt ttaccgctcc ccgtaaaatg gcatacaagc ccttcaccac caaggaaaaa
    14641 cgatttccaa tttcccaatt tttctattct ataagtaagt gaatcttgaa acgctacaat
    14701 gtgccctgtg tcaacaataa gactttcact tacagtgagc tcctcaatac ccccatagct
    14761 tgcaataagg agcgggccat caccgctgat ttgcaaaaag aatagtgact ccccactaaa
    14821 aaaacctttt aatccctgaa actttgtatt tatctctaaa gagggactgg ctgcaagaaa
    14881 tgaacttgac tgtacaaaaa ccgttccggc aatatcaagt ttcttgatat caccaggcaa
    14941 ggtaggagca agggtaacct cacctctcga accctgcgca gtaaaagtgt ttagccagaa
    15001 tgaggtacca ccaaaaaaag caatctttgc tgatttcata ataccactgc tcgatgcctt
    15061 ttttgtttct atggagacat tgacgctttt acttaccata gcaccgtctt cagcttttat
    15121 ctgctcatca gcattgagcg atacaacagc aagtgcgtat gacggattat acttaatatt
    15181 aatattcatg gctcacctcg ggagcagttt agtaatccag ttaataaaac tattgggcac
    15241 acgcgattgt atccacaatt ttcccgaacc agcaaactca caaaccaacc cttcaccgga
    15301 aaagaaagta gatttccaac caccaacctt cttaaccgca aaagaaagag aatcttcaaa
    15361 tgctacaata tgcccggtat ccaccacata gttcccacta acaggaattt cgataattga
    15421 accgtatgat gaaaaaaata aatcgccatt accagacacc ctgagcaaaa atagcccttc
    15481 tcctccaaac aaagaacgaa gaccaccaaa ttgtggagta acagtcaact caggtgatga
    15541 acacaggtac gaaccagatt gcagcatgat tgcctcaccc aacatcttct tatgttgtat
    15601 atcaccaaca tactgcggag caatgccaat ttcaccacca ccatgagatg ctgtaaaaat
    15661 atttacaaag agcgattctc caccaaagaa ttttcgagca acagcagaca gaaatccact
    15721 gccaaaacct gtctcaattg aaatttccgg gctcatataa accattgcac ctgcctcagc
    15781 tttgaccttt tcatctggag caagtttcaa tgcaaggagt gtataggtag ggttgtgttt
    15841 aaacacgtac tccatgcgaa cctcctttga atcatgatat cattcctatc tttttataat
    15901 gcctataagc tttataacct gcattattgt gtatgtcaag tttttatggt atagctgtca
    15961 cctcaatgcc caaaaagtat atcaatatct taatccccgc catgctcacg aacccactga
    16021 atagcatact gcaacaaatc ctgagatgag tccagatgca atttcatctt tatcttttca
    16081 cgaacatttt caacagtttt aacacttata tgcaactttt ctgctatatg cttgttgccc
    16141 agaccatgtc ctatcaactg aaatatctca aactccctgt tggtaagacc ctccaccgag
    16201 ctgtcaataa cacctctatc cgacaatgca ttaactatca gctcttttac ctcacctgac
    16261 aaataaatgt ccccttttaa aaccgtatag atagcattga taatcttatc aggtgcttca
    16321 ttcttcatta aatatcctcg tgcacctgct ttaatggcac gttctgcata aagcttttca
    16381 tcatacatgg aaagcaccaa tgcaggcaat tttggaaagc gttgttttaa tgctttcacc
    16441 aattctatgc cactggttcc atttaatgag atatctacca gtacacaatc aggcaccctt
    16501 ttattaatga catcaattgc ctcatcagcc gaacgtgctt cgccaatgac ctctaaattt
    16561 ttgtggcttg caataagctg ctgcaatccc tgccgtacaa taggatgatc atcaaccaca
    16621 actatgctga acttcttagt gcttttcagc ttctcagtaa caggctttaa acttttatgt
    16681 attgtgcact gaaccagcgt cccgcccgtt ttattgttct gaatctgcaa tgcagcattg
    16741 ataatccatg ccctgtattg catgatatgc atccccatgc cttttgttac atcaacatca
    16801 tctggtatgc cccgcccatc atccataatc ctgatgcaat aatcattgtc atcctcgcat
    16861 actgatacgg caatatgttt gggattacca tgcttaactg cattgttcac agcctcacgg
    16921 acaatatagt aaacatgtac cgtgtcaacc aacgcatcaa tattgctggt cacatcccat
    16981 tcaagaatgg ctttcatctt aaaatttttg tcaatatctg cgataatctc ctgcagggaa
    17041 acatgcaatc ctttgttgtt taaatcaacc gggctcagcc cctgagcaat gaggcgggta
    17101 tgttcaatag cctgatgcac catactctgt atttctacaa tttcgggcac ctcattggaa
    17161 aattcatctt ttaatttaag ccgcagcatc tctgttttaa aaattatgcc tgcaagatgc
    17221 tgccccaacc catcatgcaa atcccgcgct atacggttgc gttcatcaat gtttgcctgt
    17281 aaaatctttt tttccagctg cttcttttcg gtcatatcaa tgccaatgag cataaaatag
    17341 ttaaatacat cggcatcata gacggggcgg gcatgccatt gcacaatgcg ctcggttgca
    17401 tctttcatta ctattgaggt ttcaaaaatg tgtgtggctt ttgttttttt catgtgcgat
    17461 agttgatttc taaatgcacg ttgcccaaaa tcagggataa acagcgtaca gcagcttttg
    17521 ccggtaacct cgctggcttt gtatcctgta gcctgcaaca tggctttatt gcaggtaata
    17581 acagtaccct cttcgctcaa tgttaaaaag taagctggtg ctatatcctc cagattttgt
    17641 ataatgtcaa gtccctgctt taatttttcc tttgtccgtt taacatcagt taaatcccgg
    17701 gcaatgattg agcagcctat tatggtgccg cgttcatcct caagcggggt tacggtcatt
    17761 gacatattat ggattgcgcc atgcctgtct accccttcag actcataatg agctatccct
    17821 tcattatgtt taacccgttc aatgagctgt tcaaattcat caggacggta tggcggcgac
    17881 agcaagctta catgacagcc agctgcctca tcaaacgcat agccaaagat tttttctgct
    17941 gctttattcc agcttgcaat tgtcccatca agagttgttc caataattgc atcatccgaa
    18001 ttgtccagca aaatagatag ctgcgtgcgc acctgttcta tctgttttcg ttcggttata
    18061 tcacgggcta cgctcaacac tgcattttca tcacgataat agattgttgt gctggcaatc
    18121 tcaacgggga tggtgttgcc atggctatca acaaaagctg tttcatacca atcggtattt
    18181 tcagagatcg actgcaaaaa tgtatgtatc cggttactat cgccctcaga ggtaagctgc
    18241 tgcaacgtca tcatggcaag cgattcatgt gaaaacccca aacggttgca tgttgtagta
    18301 ttaacttcaa gaatgttccc atcacttttg tggataataa ccatatccga tatgctgtta
    18361 aacaatgact tataacgcat ctgcgatgct aaaagctcac cggtcctttg cagcaccata
    18421 tcttccagca gatccttttc tttcctgagc atatcctcag cagattttat acgcatcatg
    18481 gcatttatct gcgctatcag ctcggcctga tctattggct tggttaaaaa tgcatcagct
    18541 cctaatttaa gccccttaac cttactgtgt gtatcagtat gtatggcggt aagaaaaata
    18601 accggaatat ttcgtgtcat gggatcatct ttcaaaatgc ggcatacatc aaagccatcc
    18661 atccccggca tcttgatatc aagaataata acatcaggat ttgattgctt tgccttttca
    18721 attccctctt tgccatcctc agcagtaata accatgcaat cagcaataag attttttaac
    18781 aaagcagtaa tgcttaccag attatccttt ttatcatcaa taaccaggat tgtactcatg
    18841 attggctaac ccaatgctga acttttaaaa caaattcttc tattggaaac ggcttctgta
    18901 taaaatcatc acatcctgaa gccataatga ctgagcgctc atttttaagc gacgatgcgg
    18961 taacgatgat aaccggtata tgttttgtat ggtcatcgct tttcagctcc ttcaccacct
    19021 ctaatccatg taaatccgga agcgatagat caagcaatat acaatctggc tttacttcat
    19081 gggcaagcgc tatacctctg gttcccgact gggtttcggt aatatcgtat agttttgaca
    19141 acagagcttt caccaccaca acgctgtccg aatcatcatc tatcaccaaa atttttttgc
    19201 gatctgcctg cggttttgct tccacctgta atggcaactg caccgtaaag cacgaacctt
    19261 tcccaagctg actttcaaca tggatggttc cgccaaggat ggataataat ttcaatacta
    19321 tggtaagccc cagccccgtc cctggatatt ttgatgacag cgagcctgat gctttggtaa
    19381 aacctgtaaa caatgtatcc agaaattcac tgcgaatacc tattccggtg tcggctatgc
    19441 ttactgtgca catcccgtca tgtgcatgcg ctgacactgt tatacagcct tcctcggtaa
    19501 acttcaccgc attatcaata aggtgctcaa acacctgata caccctggtc tcatcactct
    19561 gtagtataac atcatccaga tgcgtatgca gcaaaagtcc ttttttctcc agtatacctt
    19621 cataccgttc caaaatatcg ctcaatattg ttgaaaccat aacatcagtt atatgcaact
    19681 caatgccgct atctatctca aatacttcta taaggtcatt taccagtgca aggaggcggc
    19741 taccattgcg ttcaataatt gctaaatatt ccttttcttc cggggtaagt ctgccatcag
    19801 cctgcataag caaaaccttt gacagcgtta atatggagtt taacggggag cgtaattcat
    19861 ggcttatgct gtgcaaaaat tccttattca tgggcttttt gctgaaaatt catttttatt
    19921 aaatatgcaa tgttaaacaa atttgttatt ccgccaacaa accgttcttt aatcatccct
    19981 ttgggcaaga ggtcaattat cctgttatat tcctccagcc tggtattgat tgtagcaaca
    20041 atgttcgata gctcctgata tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nacgatattt ttatagttaa aatagcatag ctttttttgt aaagaagaat taatgcccta
    20221 ctctctgatt ggcaaaacaa gaaggcttaa cgggtttgcc tgcacaccat gaaccataag
    20281 cgaaacatga aggtgcggcc ctgttgacat gccggtgctt cccacagcgc caataagctg
    20341 tccggcaacc accctgtccc cggatgaaac catcagcttt tccatatgca tgtaatagct
    20401 gaatatgccg ttgccatgat tgacgatgac catgttgcct tcgtaaaaaa gtttatctgc
    20461 aagcaccaca atgccgtcaa gcattgcata caccgggtca ccactgttgc cattcagatc
    20521 aataccgcgg tggatacgcg tcaccgtctg ccttttttta gtagagctat catacagcag
    20581 atatcgtcgt tttgcccaga atgctgaatt gatggtatgg gtattccgtg gatggtaggg
    20641 aatgctttgt atgcagtcat caaaccttgt tgcaaatgct ttttgctttt tctttgtgca
    20701 ttcagcaata aattgtttta cctcgggagt ctctatgtaa tccttctggg aatacttatc
    20761 aaactccatt gggataaccg aaacgggaaa aaaagatcgc ttaatagtta caggaatctc
    20821 aatatgaaag gaatcaccat atatgatgca atgataaacg cctggtttta actcaggatc
    20881 aatagcaaaa aatcctctgt acccccacgt tttttttgat aatacaatct ctttatcaat
    20941 aaatttcaca gaaaccaatt gtacatcctt agcaggtatg atttcacaat acgccacatt
    21001 gccctgtgca aatttttttg catagatagt acaggtaaca gcatcgttac tgacagcaac
    21061 cttttccggc atcactaccg taaagttatc cggcaaaggc tttttggcga tagttacctt
    21121 tgtaatatct cgtgcgcatg aaatggctgc acagcatacc atgattattg cagttacttg
    21181 catcttcatg atgatagcac tccctctaaa aataaaatgg tgcacaacca gtttccctct
    21241 ccttattcaa ggagagggat gaagggcgag gtttgtgaac ctgccacata ctattttatc
    21301 actatattat tgattttttc atgctaatat tgtataatac tatcatagca ctataaccat
    21361 tttcaacatc tatttgtgga ggtcactatg agcatacaga tttcattcag caaactggaa
    21421 aaagaattca tgccgcaatt cagggaacgg atcactacct ctgaggatat cgttgatgta
    21481 cagaagattt tttcatatac cataaaagag atgttacaaa aagcttttga aaaagaaggg
    21541 attacaataa acgaggagaa tatagcactt accagcagcg caccttacta taccataacc
    21601 attaataatc actcaataaa tgaactatgg aacaccagtg acttaaaaga cattttgcag
    21661 cgctttgccg aggttgctca taagcggtac attcatttga aaaaaaatat ggcaaaaaca
    21721 aagatgaaga tacgtcggta aagaattata ctattttgct aatttttcca ttaccttttt
    21781 tcggtattca taatcaggaa catgtatccc tttttgttcc atgagtatgg tataacgcag
    21841 ttgttttgca ttaagctgtt ttacaagccc ttccagccgt ttatcatcat cgcagcaggc
    21901 gttgataagc tgctgtaatg agtatatatc ttttcgcagt tctacctctt caggaatgat
    21961 gcctgcattc ttcattattt tatatgccat cctgatatca ggcggaatag tatccagttc
    22021 atcccgtgga agaggtttcc ccatccccgg aaggttatca aactcacctc gctccatggc
    22081 gtcacggata cgctgttcag ctatcagttc aaagatgtac atggatttca gtagcctttt
    22141 ttagaacgtt cttaaaaaaa attctattgt aaattatttc ccattcagca ggggtataga
    22201 tcaaacaatc aacaggcaca caaacgtatg actcaatgtc attgaagtca tcataccgat
    22261 caaaaaaacg tttatccgtt tgtttgatga caatcaagtc aacatcactt ttccctgtct
    22321 gtgtattacg agcaaatgga ccaaagagga taaccctatc aacacccttt gacaaaaagt
    22381 tatgctgtaa ttgcgatata acatgttctt taagcaccat aacagtaaag taacgatata
    22441 gtgacgatga tgtcaatagt atttttttaa caatatgccg ctataccgta atccatcaac
    22501 ctcttttgtt cgataaataa aaaaaggaag cgaaaaatat gaaacctcta ctctatatag
    22561 tacctattct tatgctgtca catcttgcat ttgccaatga gtactggcag cgccagatag
    22621 agtctttgca aaaagaacag aaacaggtgc aggcacagcc atatacctcg tttgtacttg
    22681 aatacattga tgctcgttta gcaatggcac aggaactgtc tgcactgtat catgaaagct
    22741 taaacaacaa acacagtgag cacgccacaa ccacccatta tgtaaacgtt ttatccagat
    22801 gttttgatga tgcagccatt gacataacac gacagcaggt tgtacaacag ttgcaactat
    22861 tgcaaacatc tgatgcaatt gattgcacca ccaatgacta ttatatcatt cacaccgagc
    22921 tggtgcttgc ctccgtaacc
//
LOCUS       DAOSOX010000017        22172 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2344_length_22172_cov_8.279875, whole genome shotgun sequence.
ACCESSION   DAOSOX010000017 DAOSOX010000000
VERSION     DAOSOX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_22172_cov_8.279875"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLX22_02420"
     CDS             <1..308
                     /locus_tag="PLX22_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18789.1"
                     /translation="WGDSYFKMKIPFIISYHSKDKTVQHVSLLLSFLEYTNDESINEL
                     RIGWIPVYRNVKDKKQYDDKKKVSLRLQQIEGGYNSMVAYGSTTSYYNDSTGCMVRF"
     gene            395..1201
                     /locus_tag="PLX22_02425"
     CDS             395..1201
                     /locus_tag="PLX22_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT18790.1"
                     /translation="MIKKLRDVIVNVVGGVGKNTLHIVYMMGYSTMLFLEVVYYSRSA
                     FDKRKEILKQMYYAGVKTFIVVSIVALFTGMILALQIGLELKPYQQQPLVGNIIIATL
                     TREMAPFVTAIILIAAVGSTMAAEVGTMAVSEEIDALEMMSISPVRFLVMPRVISLAL
                     MLPIATVYTNLLGCIGGGIIAHFQLNVSFDVYYSHILESLHFKATYVGLLKAFIFGIM
                     VSTISCANGLRATNGALGVGKATRNSVVSSFLMVLIVGYYITGIFYGKAG"
     gene            1228..1974
                     /locus_tag="PLX22_02430"
     CDS             1228..1974
                     /locus_tag="PLX22_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT18791.1"
                     /translation="MIEFKHVTKILNGVKVLDNLNITIEKGETFVIIGRSGTGKTVTL
                     KHIAGLLEPESGAVLIDGKQMNGASRRQKDSLREKIGFVFQSGALINWMTVEENIALP
                     LVEHKVLSADEIKQIVEEKLDLLQLTSARDRMPAEISGGMKKRASLARVLVRNPEIIL
                     YDEPTSGLDPVMSSLINTIINQIKKDQHITQVVVTHDMESAYAIGDRIAMLYDGSIIE
                     CDVPDAIRTTTNPIVRQFISGSLEGPIEVL"
     gene            2066..2719
                     /locus_tag="PLX22_02435"
     CDS             2066..2719
                     /locus_tag="PLX22_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT18792.1"
                     /translation="MKQEMAVGIVVILAMTVLGYFTIIMSGEIIDTHTYYPASIVFKD
                     IEGLAKDDKVRINGVLSGYVAGTQLLDNKVIVTLKLFNHFTLYENYEIMIRNETALAG
                     KYVSIDPGTPVDENGKQYAVVTTRENLSGIAVSDPFTLLSRLIADNRGNVNSVLKNIR
                     DITDKINSGKGTLGKIINDATVHDQASGLIKELRDTIEDTREQAPITSFLRAALTAF"
     gene            2848..>4868
                     /gene="carB"
                     /locus_tag="PLX22_02440"
     CDS             2848..>4868
                     /gene="carB"
                     /locus_tag="PLX22_02440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOT18793.1"
                     /translation="MPRRTDITKILIIGSGPIVIGQACEFDYSGSQACKALREEGYTI
                     VLVNSNPATIMTDPEIADRTYIEPITHDIVAQIIERERPDALLPTVGGQTALNIAVQL
                     YDSGTLQKYGVELIGAKIEAIKKAEDRDLFKKAMLSIGLQVPRSVLASSIDEALTVLE
                     SISLPIIIRPAFTLGGTGGNIVYNQEDFIELVRRGLDESPISQVLLEESVIGWKEFEL
                     EVMRDLNDNVVIVCSIENFDPMGIHTGDSITVAPQQTLTDREYQVLRDMAIAIIKEIG
                     VETGGSNIQFAVNPDDGRIMVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGLTLDE
                     IRNDITRETPACFEPTIDYVVTKIPRWAFEKFEGADTTLGTQMKSVGEAMAIGRTFKE
                     SFQKALRSLEIERYGFGSDGNVKIDAMLDGLSENKKNDIIEMNLRIPKENRVFYIKKA
                     LELHWSVDTIYSLTSIDPWFLHQLKELVDAEHDCIAQLNSGLSKDIIVRMKQWGYSDR
                     QLAYLAHKDELTLLYAKGIDSQKDYSKRLSELEDDIRNYRQKNGIVPGYRLVDTCGGE
                     FEAYTPYYYSSYDDEDEARYSDKRKIMILGGGPNRIGQGIEFDYCCCHASFALRDAGV
                     ESIMVNSNPETVSTDYDTSDRLYFEPLTLEDIIHIYQKEKPEGVIIQFGG"
     assembly_gap    4869..4968
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4969..6060
                     /locus_tag="PLX22_02445"
     CDS             <4969..6060
                     /locus_tag="PLX22_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT18794.1"
                     /translation="GGRAMAIMYDEKSLIEYIVSAAELSPEHPILIDKFLDDALEIDV
                     DALCDGMDVYVAGIMEHIELAGIHSGDSACSLPPVSVKPVMMHTIMATTAALAHELGV
                     IGLLNIQFAIKNDVLYVLEVNPRASRTVPFVSKATGVPLAKIAVQILLGKKLKDFGLT
                     QANTMPYCSVKEAVLPFNKFPGVDTLLSPEMKSTGEVMGIANNFGEAFYKAELGAGDT
                     LPLHGTIFLSINQRSKEELLPEMRLLYDAGYKLIATEGTAAYLNAHGIPCEKVYKVSE
                     GRPNIVDVIKNKQVDLIINTPAGKVPKEDAYTIRQAAVRYHVPIITTIAAAKAAVQGM
                     IFTKKAGHFTVRSLQEYHAEVQQCVKKRF"
     gene            6039..6578
                     /gene="pyrR"
                     /locus_tag="PLX22_02450"
     CDS             6039..6578
                     /gene="pyrR"
                     /locus_tag="PLX22_02450"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT18795.1"
                     /translation="MCEEKILMNKQQIDESIKKISTDIINEFGDMANVTLVGIQTRGV
                     EIAKRIKKLLELHGGNEIKMGTLDITFYRDDLATRGVIPAIKDTIIEFNITKKIVILV
                     DDVIFTGRTTKAALETLMSFGRPQIIRLAVLVDRGNRELPIQPDYCGIKINTKLNDKV
                     KVRLKETDGIEDTVLYCTE"
     gene            6593..7468
                     /locus_tag="PLX22_02455"
     CDS             6593..7468
                     /locus_tag="PLX22_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOT18796.1"
                     /translation="MKPTYHCIPCHVNHVYRIISQLSPEADKAHSIMQQLTLMIAKNR
                     YDTPPDCAYVLYKKIKVLTGIDDPFANVKQQNNDIMLSLMPQLQSVLDDDEPIKQALR
                     ISLAGNIIDYGIGDVDYSGYLKKFQDLVHDIDIPDKFYNQFIASVANAKKILFIGDNA
                     GEIVADKLLVEKLNHDNITFVVRGGAVINDATIDDAHYVGLSKKVKIVTTGDRTPGIH
                     LHRSGKDFLQELLHADMIIAKGQGNFETMIDASLQDFVKHGATLFFLFKIKCVPVAEY
                     MGKKVEDNAFVIREL"
     gene            complement(7512..8774)
                     /locus_tag="PLX22_02460"
     CDS             complement(7512..8774)
                     /locus_tag="PLX22_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOT18797.1"
                     /translation="MTHHNKLFGLSPEAGRWIYVITGLIICLCLGSVYAYSIFLNPII
                     NEFSTESYKLSASQGNLPFMVFLAFFSILMFFAGKIMDKLGPKKLTIYGGLVVGLGWA
                     LSYYAVEAKSLNLLILTYGVIAGSGVGVVYGCPLNIVGKWFPDKKGLATGLVLAGFGG
                     SALITGKIANVLIQQSSLSLTFLYFGIGFAILLVLLAFPFKSPEAGWKPQGWTPSASA
                     ASTVDFTSSEMVKTSTFWGLLLAYLIGCLAGLMAIGISKPVGSEIIKIDGETATTLVG
                     IFAIFNALGRPLFGWLTDKISPKGASILNLLIIIAVSIMMLVAKEGDVTMYIIAFCGF
                     WLILGGWLAIAPTTTLTFFGVKNYSRNYGIVFFAYGLGAILGGIISGQAKDAFGSYQV
                     AFWPTLILGVVGLMIVMATLKPPVKKAA"
     gene            complement(8889..9890)
                     /locus_tag="PLX22_02465"
     CDS             complement(8889..9890)
                     /locus_tag="PLX22_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOT18798.1"
                     /translation="MKKGLLFLGGCIAIIATMISFEMFGKSDTQITAEEVNQPINAPE
                     FYKGIYLNSQSGRDLTILKNLITRAKDAGFNVVVIDVHNESSNKFLIPKENIDYVTAQ
                     GFYPIARIVMFPDGLKVYPIPEELIKGRLELAKKACEIGFREIQFDYIRFNDYGILKH
                     VPLEERYKTVEGFLARAKSELLPYKVKVACDVFGRIPLNTNDSIGQRMEGLDRVADVI
                     CPMAYPSHYTWSKKMMSDPYYTVLITGKRAKERTKNAEIVMFIQAFKIKVSMSGLSFQ
                     EYMTKQIQAVHDSGVKGFIFWNASQQYDEPIAATRQFYSNFKSQNISKSENPKMETM"
     gene            10182..10616
                     /locus_tag="PLX22_02470"
     CDS             10182..10616
                     /locus_tag="PLX22_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOT18799.1"
                     /translation="MRLWSLHPEYLDPKGLVAVWREGLLAKKVLGGKTKGYTNHPQLL
                     RFKNYNNPMKAIDSYLFWIYKEAQKRGYHFAAEKINEELLIAIIPVTTGQLEYEIDHL
                     LKKVYSRNLNFYDRLLDGKNKIICHPVFYCVEGDVEQWEIKK"
     gene            10598..11854
                     /locus_tag="PLX22_02475"
     CDS             10598..11854
                     /locus_tag="PLX22_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT18800.1"
                     /translation="MGNKKIKSAVITNNLFRSLKVYNFRLFFIGQFISLIGTWVQQVA
                     SSWLIYQLTSSSMWLGLSAFAAQIPIFVMTPLTGVVADRFNRHAIFMITQTMLCIQSL
                     ILGLLVIFDYITPVSIVLLNLTMGCINAFDMPVRQSFLFDMVADKQLLSNAIALNSVV
                     FNSTRLIGPPLAGFLVAQFGEGLCFLINTTTFAGIILALLLMKDIHYSKILHTSDFKE
                     NIKEGFVYVKNHYPIKSVLILLGLMSLIGMPFMVLMPVVAVEILHGDSHTLGFLLGTA
                     GFGALVGALFMASRSSTTRFENKIAAGSLLLGSTLVLFSFSRNLYCSLILIFIIGISL
                     IIQMASCNTFIQSMVDDTMRGRVMSLYGMSHVGMAPIGNLIAGAIAQKIGVAHTLLLC
                     GTGCAIVALWFYKNLHKLKKHIVRYV"
     gene            12014..13360
                     /locus_tag="PLX22_02480"
     CDS             12014..13360
                     /locus_tag="PLX22_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT18801.1"
                     /translation="MFLYYKIKFQVMNKDKLLDKSINLSCNNCIQKNNTLNGDSWDFK
                     NANTKEYTHCFHSYPAMMIPQVARRIIEIYGKNATLLFDPYCGTGTSLVEANLKGINA
                     IGTDLNPLARLIAQAKTTKIDSKALDKYLYDFTNYLFSKSFKEEDYSTITLPCITNID
                     IWFSKSVQYKLAIIFNYIIQKIDNVFIQNFFKVAFSETIRETSNTKNGEFKLVRDKKL
                     IKTEDLDVFGIILGKLSRNKEGLINFIKVCPQNVVSHIYSFDTVLSIPYDLVPQESVD
                     IVVTSPPYGDSRTTVAYGQFSRFANEWLGFHNANKIDNLLMGGRKNNVNNFKFNNDLL
                     NDVITAISNKDNKRAMEVVSFFKDYESSIVNVSATIKKGGVVCYVVGNRTVKNINIPT
                     NKITAQLFEQNNFVHMETIVRNIPNKRMPLMNSPTNIPGQTGQTMKNEYIVICKKI"
     gene            13364..14065
                     /locus_tag="PLX22_02485"
     CDS             13364..14065
                     /locus_tag="PLX22_02485"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOT18802.1"
                     /translation="MQLKKLVKNLEDIKSKGFIETIRKGPTAVGYLLEKELGLSETNI
                     TIPDIGGRIEIKATRKNTNSLITLFTFNRGVWRMKQKDLINKYGYIDAQGRQAIYATV
                     SANVHNAQGFFLSIDNDKHLVLLKNAKEDETIAEWSTYVIAGKFMTKLDRLLLILADS
                     KMVDEKEFFHYNEAYILENPTPEKFLEGFNRNELMIDLRMHLKDYGGVRNHGTAFRIS
                     EKNLTDLYTKKRRLI"
     gene            14215..15333
                     /locus_tag="PLX22_02490"
     CDS             14215..15333
                     /locus_tag="PLX22_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOT18803.1"
                     /translation="MQHITWIPFDIATRYMVDVFTGLGVPHDDALICADVLITADKFG
                     IDSHGVNRLKPIYYDRIKQGIQKPVTQCAVVKEGPTTAVIDGNDGMGQVIAYRSMQMA
                     IDKAKKYGMGMVTVRNSTHYGIAGYYAKMAADNAMIGITGTNARPSVAPTFGVENMLG
                     TNPLTFGIPADENFPFLLDCATSVSQRGKIEVYARLDKPLPPGWVIDNHGNSCTNPHQ
                     VLDGLIKGSMALTPLGGIGEDGAGYKGYGYCTVVEILSAALQAGSYLKMLSGFDMQGN
                     KVPYHLGHFFIAIAIEAFTDIASFKSITGDILRQLRNSARMPGQKKIYTAGEKEYLTY
                     LERKDKGVPVNNEVMKELVAMRDELNITGYELLTLHAS"
     gene            15349..15612
                     /locus_tag="PLX22_02495"
     CDS             15349..15612
                     /locus_tag="PLX22_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOT18804.1"
                     /translation="MMANENHSNDEFPREITFKVIFHNTLFLKEMLINICAENTIDAH
                     ITERESKQGKFISYTITALFAHEEVLHAICSHITRLDGFITMF"
     assembly_gap    15784..15883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15957..16406
                     /locus_tag="PLX22_02500"
     CDS             15957..16406
                     /locus_tag="PLX22_02500"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOT18805.1"
                     /translation="MSILTTIESNLKDAMKSKNELLSSTLRMMKSDILYEKTKSTQEL
                     TDEKIIEVLTRSAKRRKEAIAEYEKAGRDDLARKEKQELEIIMQYLPQQMSADEIASH
                     VDAVIASMGTITNKDTGKVMGQVMKDLKGKADGQVVKQIVAQKLEKL"
     gene            16430..18199
                     /gene="dnaG"
                     /locus_tag="PLX22_02505"
     CDS             16430..18199
                     /gene="dnaG"
                     /locus_tag="PLX22_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT18806.1"
                     /translation="MAIPRDTIEHIRQKAAIEDIIRRYVPTLTKKGKNYVGLCPFHKE
                     TSPSFTVSPDKQMFYCFGCHEGGNVFTFIAKVEHVDFVQSVKIVGEIVGIPVRNEEKQ
                     NDEYTTLLAINAMIAERYRQALMSAAGKRALDYLLHRGVTRQGIDEFQLGYAPDEWQY
                     ATDILRQKNVPLDIAVKTGNINKSVKDSTSHYYDRFRNRVIFPIFDQKNEIIGFGGRI
                     IESGEPKYMNSPESLVFQKRNILYGFNKARQHIAELGRAIVVEGYLDVIGVYQAGIQN
                     VVAPLGTALTQEQLQLLSRLSNEVIIVFDADSAGVKASLRSLDVAANLNITIKIAQLP
                     EMDPFDFVNKRGAREFMAVVDSALKPVDFRIERIIAENKGKSQVNVLKQIFGILRTIP
                     FEVERQHYFKKLSSVFQVDEKAIRADYERFTKGKTTNGVIVTDNNTVDFYAKSQRELV
                     LLLCNYPLLIENAIVDCPLQDFTDTVAKNIYQTLIALYEDNESFSLEKMFDFYPEGEE
                     KKLLTQGFSKQFSFEDPHVAYSEIILNMKLFTIDKKINEFAEKIEKDNRENIQYYITE
                     IEVLRREKEKLTSYIYNKGSIKH"
     gene            18310..20073
                     /gene="rpoD"
                     /locus_tag="PLX22_02510"
     CDS             18310..20073
                     /gene="rpoD"
                     /locus_tag="PLX22_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOT18807.1"
                     /translation="MAKGDVLLESIPEVKKLVELGISNGEITYDELNDILPDKILNSD
                     KIDDIFILLNQLGIDVVEEPTRRNDAAPLKKEKTHSKKSSSSSSETGVSDDPIRLYLK
                     EIGKVSLLSGDQEVKLAKRIEEGEILIENAVLDSTVLLNELVKNHSKVKSGKIKLTDI
                     LRINKLYYFSSFDMHDLEKRYENNMSIIIAEDKKIMQSQAKLKKLSEDSKKALELKEK
                     MMASRSSIREALINLQVHENEINKTAQRILSMVARIKETRHFFNNLENSFNKTVKELK
                     QFGRKLEKNEDVKKILKELKIKNKDELLEIVKDIRNNERKIRRIEQEAGSTCDEIMEW
                     GTQIEIGHRKISLAKKELINANLRLVVSIAKKYANRGMHFFDLIQEGNIGLIKAVDKF
                     EYRKGYKFSTYATWWIRQAITRAISDQARTIRIPVHMIEQINKVMRETRLFQQEFGRE
                     PSPDELADRLGWHVSKVKGVKNVAREPISLETPIGEEEDSLLGDFIEDKEVDSPVNTA
                     AYRLLSEQINAVLSTLPAREQKVIRMRFGLDDGYSHTLEEVGYVFKVTRERIRQIEAK
                     ALRRLRHPTRARKLKDYLDYM"
     gene            20200..>22172
                     /locus_tag="PLX22_02515"
     CDS             20200..>22172
                     /locus_tag="PLX22_02515"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT18808.1"
                     /translation="MHHQENTNLIKTVTIVGAGSWGTALANVIGHNHPEMTVRMWAYE
                     KDVVRTINNNHINTAYLPGIILADSIIAYHDLKESVKQTDAIIIATPSKVVYDICVKL
                     RRHIQPDTYCGYLSKGFCKIGSGIYTMSQTIELALPFLMDTVCAVYGPSHAEEVCKEY
                     HTALHIASKSNSSINFFMNLLSNEYIECIPINDIIGIDCGSTLKNPAAIAAGLISNLP
                     QCGDNLMGALIAQALKEMVQLGKCLGGQEDTITGIAGLGDLVATALSQHSRNRRFGKE
                     IGEQITQKGTTLSLFDRFLLRFKPDDVLGRMGGRVHYLVEGAYAIEPIIELADRYTIT
                     MPIYRSLYEVLLNKRDPWLLIETIKNPAKYEILTRRARIKAKERKKGIERMSGLIFKN
                     IVVERMIKELSVPEKKREVLDQKNEYIQLLHQYKHLKKPKKFLYELSLYNNLNEKNYE
                     EQLKTIIEFYYTSISDRYVYSFSLLMLKAARLFFYIYGLLYRRKIVEFFEERISLSGD
                     IKYLKKIAMTANPVYICNANQIADSIFVVLALIKFITIPLPRFNINSQLLKNSLLRYL
                     FRVSGGYIVDKTRYSSTLYRETLLNYILICVEHGITILYSNDSSAPNDETTDSISQND
                     IIQDVLIERLSILLQQTSEEIAIIPVEVAHKYY"
BASE COUNT         7127 a         3671 c         4618 g         6556 t
ORIGIN      
        1 gctggggcga tagttacttc aaaatgaaga ttccgttcat catttcatat cacagcaaag
       61 ataaaacagt acagcatgta tcgctgttgc tatcgttttt agagtatacc aatgatgaat
      121 ctatcaatga gttgcgtatt ggatggatac ctgtgtatag aaatgttaag gataaaaaac
      181 agtatgatga taaaaagaag gtgtcgctac ggctgcagca aatagagggt ggctataatt
      241 ctatggttgc ttatggaagc accacgagct attataatga ttctacaggt tgcatggtaa
      301 ggttttaaat atatggtgaa tacatctact gtttcgtggt ataatggtgt attgcataat
      361 cagtttagtt tatttttaag gtaagcatat ggtcatgata aaaaaattaa gggatgttat
      421 agtcaatgtt gtaggtggtg ttggcaaaaa caccttacat attgtttata tgatggggta
      481 ttccaccatg ttattccttg aggttgtcta ttattcacga tcagcctttg acaaacgtaa
      541 agagatttta aaacagatgt attatgcagg ggtaaaaaca ttcattgttg tttcaattgt
      601 agctcttttt accggtatga tccttgcact gcagataggg cttgagctaa aaccctatca
      661 gcaacagccg cttgtaggga atatcatcat cgcaacgctt acccgtgaaa tggcaccatt
      721 tgtaactgct atcatcctga ttgcagcggt tggctcaacc atggcggctg aggttgggac
      781 tatggctgtt tccgaagaga ttgatgcact ggagatgatg tcaataagtc ctgtgcggtt
      841 tctggttatg ccacgggtta tatccctggc actcatgctt cctattgcaa cggtttatac
      901 aaatttgctg ggatgtatag gcgggggtat catagcacat tttcagttaa atgtaagctt
      961 tgatgtgtat tattcacata ttcttgaatc attgcatttt aaggcaacct atgtagggct
     1021 tttaaaagcg tttatatttg gcataatggt ttctactata agctgtgcta atggattacg
     1081 ggctaccaat ggggcattgg gagtgggtaa agcaacgcga aactcggttg tttcgtcgtt
     1141 tttaatggtg cttattgttg gttactatat cacgggaatt ttttatggga aagcaggata
     1201 atcgatagtt attgttgata gtgtactatg attgaattta aacatgtaac aaagatatta
     1261 aatggcgtaa aggtgcttga taacctcaat ataactattg agaaaggtga aacctttgtt
     1321 attatcggca gatcaggaac agggaaaacg gttactctga agcatatcgc tggtttactt
     1381 gagcctgaga gcggtgcggt gcttattgat gggaagcaga tgaatggcgc cagtagaaga
     1441 cagaaggata gcttgcgtga aaaaatagga tttgtttttc agtcaggtgc tttaatcaac
     1501 tggatgacgg ttgaagaaaa tattgcactt ccattggtag agcataaggt attatcggca
     1561 gatgagatta aacaaattgt tgaagaaaaa ttggatttat tgcaattgac atctgcgcgt
     1621 gatagaatgc ctgctgaaat cagcggtggc atgaagaagc gtgcaagcct ggcaagggta
     1681 ttggtgcgca atcctgaaat aatactctat gatgaaccaa cttcaggtct tgatcctgtt
     1741 atgtccagct tgatcaatac aataataaac cagataaaaa aggatcagca tattactcag
     1801 gtagtagtaa cgcatgacat ggaaagtgcg tatgcaattg gagacaggat tgccatgctg
     1861 tatgatgggt caattattga atgtgatgtt cctgatgcaa ttcgcactac aaccaatccc
     1921 attgttcgcc agtttatttc tggttctctg gaaggtccca tcgaggtttt gtaatgtata
     1981 ttctatgcga tgggaaagct tcatttaaag tatcatgtta tttaaaatgt tttaacgatg
     2041 tgctatacta ttcaggaggt gtgctatgaa acaggagatg gcagttggta ttgttgtcat
     2101 tttagctatg acagtattag gatattttac catcattatg agtggtgaga tcatcgatac
     2161 tcatacatat tatccggcat cgattgtttt taaagatata gaagggctgg caaaggatga
     2221 taaggtacgt atcaatggcg tattatcagg gtatgttgcc ggtactcagt tgcttgacaa
     2281 taaggtcatt gtaacattaa agctgtttaa tcattttacc ttatatgaaa actatgaaat
     2341 tatgattcgc aatgaaacag cattagcagg aaaatatgtc agtatagatc cagggacgcc
     2401 tgttgacgaa aatgggaaac agtatgcagt tgttaccacg cgggaaaatc tttcaggtat
     2461 tgctgtaagc gatccattta cattgctttc aaggcttatt gctgacaaca ggggtaatgt
     2521 aaattcggta ctcaaaaata tacgggatat aactgataag attaattccg gcaaagggac
     2581 attgggcaaa atcatcaatg atgctacagt tcatgatcaa gccagtggct taataaagga
     2641 attgcgtgat actattgagg ataccagaga acaggcacct attaccagct ttttgcgtgc
     2701 tgctttaact gctttttaac aaaaaaatgc tgccctgccg ttattattgt tttttttgga
     2761 aaaaatttgt ctacgggaaa gctgtgctct ttgctttttt actcacaatc atattgtgtg
     2821 ataacacata gaaaggattc aatatagatg cccaggcgaa cggatattac aaaaattctt
     2881 attataggtt caggtccaat tgttattggt caggcgtgtg agtttgatta ttcaggttca
     2941 caggcatgca aggctctcag ggaagaaggt tatacgatag ttcttgttaa ttccaatcca
     3001 gcaaccatta tgaccgatcc ggagattgct gacaggacct atattgaacc aattactcat
     3061 gatatagtgg cacagattat tgaacgtgaa agacctgatg ctctgctgcc aacagttggc
     3121 ggtcagactg ctttaaatat agcagtacag ctttatgaca gcggtacatt acaaaaatat
     3181 ggtgttgagc tcataggtgc aaaaattgaa gccataaaaa aagctgaaga cagggaccta
     3241 tttaaaaaag caatgctttc aataggttta caggttcccc gttctgtatt ggcttcctca
     3301 atagatgaag cactaacggt tcttgaatcc atatcgcttc ctattatcat acgtcctgct
     3361 tttacattgg gtggtaccgg cggcaatata gtgtataacc aggaagattt tattgaactg
     3421 gtgcgcaggg gattggatga atcgcctatc agtcaggttt tactggagga gtcagttatt
     3481 gggtggaagg aatttgaatt agaggtaatg cgtgatttaa acgataatgt tgtcattgtg
     3541 tgctcaatag aaaattttga tcccatgggt atacataccg gtgattcaat cacggtagct
     3601 ccacaacaaa ccttaaccga cagggaatat caggttttac gtgatatggc aatagcaata
     3661 ataaaagaga tcggcgtgga aaccggtggt tctaacattc aatttgctgt aaacccagac
     3721 gatggaagga ttatggttat tgagatgaat ccacgcgtta gccgcagctc agcattggcg
     3781 tccaaggcta ccggttttcc tattgcaaag attgcagcaa aattggccat agggcttacc
     3841 cttgatgaaa tccgcaacga tatcacccgt gaaacaccgg cgtgttttga gccaaccata
     3901 gattatgttg ttaccaaaat accacggtgg gcatttgaaa aatttgaggg tgctgataca
     3961 acgttaggga cacagatgaa atctgttggc gaagcaatgg ctatagggcg tacctttaaa
     4021 gaatcatttc aaaaagcttt acgctctctg gagattgaac ggtatgggtt tggctcggac
     4081 gggaatgtaa agattgatgc aatgcttgat ggattatctg aaaataaaaa aaatgacatt
     4141 atagaaatga atttgagaat tccaaaggaa aatagagttt tttacataaa gaaagcactg
     4201 gaattacact ggagtgttga taccatatat tcactaacat caatagatcc atggtttttg
     4261 catcagctta aagaattagt tgatgccgaa catgattgta ttgcacaatt aaacagtggg
     4321 ttatcaaaag atattattgt acggatgaaa caatggggat attcggacag gcaactggca
     4381 tatttagcac ataaggatga attgacattg ctttatgcga agggaattga ttcacagaag
     4441 gattattcaa aacgattgtc cgagcttgaa gatgatataa ggaactatcg ccaaaaaaat
     4501 ggaattgtcc ccggctaccg acttgttgat acctgtggcg gcgagtttga agcatacacg
     4561 ccctattact attcgtcgta tgatgatgag gatgaagcca ggtattcgga taaacgaaaa
     4621 ataatgattc tgggtggcgg ccccaacagg attgggcagg gaatagaatt tgattattgc
     4681 tgctgtcatg cgtcatttgc tctgcgtgat gctggcgttg aatccatcat ggtaaactcc
     4741 aaccctgaaa cggtttcaac ggattatgat acctcggaca ggctatactt tgaaccgctt
     4801 acactggagg atattatcca tatatatcaa aaagaaaaac ctgaaggggt tattattcag
     4861 tttggtggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg tggaagggca
     4981 atggcaatca tgtatgatga aaaaagcctg attgagtata tagtttctgc tgcagaacta
     5041 tcgcccgagc atcctatcct tattgataaa tttttagatg atgctcttga aattgatgtt
     5101 gatgcgctgt gtgacggcat ggatgtctat gttgctggca ttatggagca tattgaattg
     5161 gctggaattc attcgggtga ctcggcatgc tcgttgccgc cggtgagcgt taagcctgta
     5221 atgatgcaca ccattatggc aacaacggct gcgttagcac acgagcttgg tgttattgga
     5281 ttattaaaca tccaatttgc aattaaaaat gatgtgctct atgttctgga ggttaacccc
     5341 cgtgcttcgc gtacggtacc atttgtatct aaggcaacag gcgttccgct tgctaagata
     5401 gccgtgcaga tacttttggg taaaaagctc aaggattttg gattaacaca ggctaacact
     5461 atgccgtatt gtagtgttaa agaggcggta ttacctttta ataaatttcc aggtgtggat
     5521 acattgctat cgcccgagat gaaatcaacc ggcgaggtga tgggtatagc taacaatttt
     5581 ggcgaagcat tttataaagc cgagttagga gcgggggata cgttgccgtt gcacgggaca
     5641 atatttttaa gtatcaacca gcgttcaaaa gaagagctgc ttccagaaat gcgtttgctt
     5701 tatgatgcag gctataagct cattgccact gaaggtactg ctgcctacct taatgctcat
     5761 ggtatcccct gtgaaaaggt gtataaggta agcgaaggac gtccaaatat tgttgatgtt
     5821 attaagaata aacaagttga cttgattatc aataccccgg cgggcaaagt acctaaagaa
     5881 gatgcctata ccatacggca ggcagcagta cgctatcatg tgccaatcat cacgaccatt
     5941 gctgctgcaa aagctgctgt acaggggatg attttcacaa aaaaagctgg acatttcacg
     6001 gtgaggtcct tacaggaata tcatgcggag gtgcaacagt gtgtgaagaa aagattttga
     6061 tgaacaaaca gcagattgat gagagtataa aaaaaattag cactgatatt atcaatgaat
     6121 ttggcgatat ggcaaacgta acacttgttg gcatacagac acgaggtgtt gaaattgcaa
     6181 agcgtataaa aaagcttctg gaattacacg gtggtaatga aataaaaatg gggacgctgg
     6241 atataacatt ttatagggat gatttagcta cacggggtgt tatacctgct ataaaagata
     6301 ccatcattga gtttaacatt accaaaaaaa tagttatact ggtggatgat gttattttta
     6361 cgggcagaac aacaaaagct gcactggaaa ccctgatgtc atttggcaga cctcagatta
     6421 tacggctggc agtgctggtt gacaggggca acagggaact gcccatacag cctgattact
     6481 gtggcattaa gattaataca aaattgaatg ataaggtaaa agtgcgtctt aaagaaacag
     6541 atggtattga agatactgta ttatattgta cggaatgata cagaattaaa ccatgaaacc
     6601 cacataccat tgcattccct gccatgtaaa tcacgtttac agaattattt cgcagctatc
     6661 acccgaggct gataaagctc atagcattat gcaacagctc acattgatga ttgcaaagaa
     6721 tagatatgac acaccacctg actgtgcata tgtgctctat aaaaagataa aagtattaac
     6781 ggggattgat gacccttttg ctaacgttaa gcaacagaac aatgacataa tgctatcatt
     6841 gatgccacaa ttacaatcgg tgcttgatga tgatgaacca ataaagcagg cgttaaggat
     6901 atcgctggca ggtaatatta tagattatgg cattggtgat gtggattatt cagggtatct
     6961 caaaaaattt caagatttag tccatgatat cgatataccg gataaatttt ataatcagtt
     7021 tattgcttcg gttgccaatg caaaaaagat attgtttatt ggagataatg caggtgagat
     7081 agtagctgat aaactgctgg ttgaaaagct gaaccatgat aacattacct ttgtcgtcag
     7141 gggaggcgct gtgataaatg atgcaacaat tgacgatgct cactatgttg gattaagcaa
     7201 aaaagtaaaa atagtgacca ccggtgatag gactccgggt atacatttgc atcgcagcgg
     7261 taaagacttt ttgcaggaat tactacatgc tgatatgata atagccaaag gtcagggaaa
     7321 ctttgaaacc atgatcgatg cttctttaca ggattttgta aaacatggtg caacattatt
     7381 ctttttgttt aaaataaaat gtgttcctgt agcagaatac atggggaaaa aggttgagga
     7441 taatgctttt gtaatacgag aattgtaaaa ggggcacata ttgttgtgcc ccttttacac
     7501 tattcattat gttatgcagc ttttttaaca ggtggtttga gtgtagccat aacaatcatc
     7561 aaacctacca cacctaatat cagagtaggc cagaaagcta cctggtatga tccgaatgca
     7621 tcttttgcct gccctgaaat gattccacca agaatagcac ctaacccata agcaaagaat
     7681 actataccgt aattgcgtga gtaattcttt accccgaaaa aggtaagggt agtagttgga
     7741 gcaattgcaa gccatccacc caggatgagc cagaatccgc agaatgcaat aatatacatg
     7801 gttacatcac cttcttttgc aacaagcatc atgatggaca ctgcaatgat gatcagtaga
     7861 ttaaggatgg atgcaccttt tggtgatatt ttatcggtaa gccatccaaa aagaggacgt
     7921 cctaaagcat tgaatattgc aaaaatacca accaatgtag tagcggtctc tccatcaatt
     7981 tttataattt cgctaccaac aggttttgaa attcctattg ccattaaacc tgctaaacag
     8041 ccaataaggt aggctaataa taatccccag aatgttgagg tctttaccat ttcgctggaa
     8101 gtaaaatcaa cggtactggc tgcagatgct gaaggggtcc atccctgtgg cttccatcca
     8161 gcttccggac ttttaaatgg gaatgccaga agaaccagaa gaatggcaaa tccaatacca
     8221 aaatagagga atgttaatga taagctactt tgttgaataa gtacgttggc aatttttcct
     8281 gtaattaacg ctgatccgcc aaagccagca agtaccaaac cggttgcaag tcccttttta
     8341 tcggggaacc atttccctac aatatttaat ggacagccat atacaacacc cacaccgcta
     8401 ccagcaataa caccgtatgt aagtattaaa agattaaggc tttttgcttc aacagcataa
     8461 tacgatagtg cccagcctaa tccaaccacc aatcctccat agatggttaa tttttttgga
     8521 cctaacttat ccattatctt tccagcaaag aacatgagta tggaaaagaa agctaaaaag
     8581 accataaatg ggagattgcc ctgtgaagca ctgagcttat agctctcagt actaaattca
     8641 ttaattattg gatttaagaa tatgctatat gcatatacac ttcccagaca taagcatata
     8701 attaaaccag taataacata tatccatcgt cctgcttcag gcgatagacc aaaaagtttg
     8761 ttgtgatggg tcatataaac caatcctcct gtatgttttt taataaatta taatttaaat
     8821 cacaaaagac aattgctgaa aacaaaaatg attagcagta taatagtcaa gaatattttt
     8881 acttattgtc acattgtttc cattttggga ttttctgatt ttgaaatatt ctgagattta
     8941 aaatttgaat agaattgtct ggttgcagca attggttcat cgtactgctg tgaggcattc
     9001 cagaagataa aaccttttac ccctgaatca tgtacagcct gtatttgttt ggtcatgtat
     9061 tcctggaatg ataatcctga cattgatacc tttatcttaa atgcctggat aaacataact
     9121 atttcggcat ttttggtgcg ttcttttgcc cttttaccag tgattaatac tgtatagtaa
     9181 ggatcggaca tcatcttttt gctccatgta tagtgggatg gataagccat ggggcatatc
     9241 acatcggcaa ccctgtcaag tccttccata cgctgtccaa ttgaatcatt ggtattaagg
     9301 ggaatccttc caaagacatc gcatgcaact tttaccttgt atggcaataa ttcggatttt
     9361 gcacgtgcta aaaatccttc aacggtttta taacgctctt ccagtggaac gtgttttaaa
     9421 ataccatagt cattgaaacg gatataatca aattgtatct ctctaaatcc aatttcacag
     9481 gctttctttg ccaattctaa tctgccttta ataagttctt cagggatggg atatactttt
     9541 aatccatccg ggaacataac gatacgcgca attgggtaaa aaccctgagc ggtaacataa
     9601 tcaatatttt ctttagggat taaaaatttg ttgctggatt cattatggac atcaataacc
     9661 acaacgttaa agccagcatc ttttgccctt gttattaaat ttttaagtat agttaaatct
     9721 cgtcctgatt gcgaatttaa gtaaattcct ttataaaatt caggcgcatt aatgggctgg
     9781 ttaacctctt ccgcagtgat ttgcgtatca cttttaccaa acatctcaaa gctaatcatg
     9841 gtggctataa tagctataca cccaccaaga aacagtaatc cctttttcat atttttccct
     9901 ttctaaaaga ttttgattaa tggaaatagt gtgtacatga tggtactatg taattatttg
     9961 tcgactaaaa tatggtatca ttgtacacaa aaactaaaac acaaagttat ggcagcaaaa
    10021 gtttttttag aagtaaacat gacagggaaa tttcccttat tttctttttt tatattgtct
    10081 ggttttgtat gtccgagctt aaaattttta tttacatatt tgcaatagca tatgataaga
    10141 ctaatacatg tatgagcgat agttattgat gatgaaatgg tatgcggtta tggtcattac
    10201 atccggagta tcttgatcct aaaggactgg ttgcagtatg gagagaagga ttgttagcaa
    10261 aaaaggtgct tggtggtaaa acaaaagggt ataccaacca tccgcaattg ctcaggttta
    10321 aaaattataa caatccaatg aaggctattg atagctatct tttctggatt tataaagaag
    10381 cacaaaagcg cggttatcat tttgccgctg agaagattaa cgaagaacta ctaatagcaa
    10441 taatccctgt aacaacaggt caacttgaat atgagattga tcatttatta aaaaaagtat
    10501 atagcagaaa tcttaacttt tatgacaggt tgctggatgg taaaaacaaa attatatgtc
    10561 atcctgtatt ttattgcgta gaaggtgatg tggagcaatg ggaaataaaa aaataaaatc
    10621 tgctgtcata accaataatc tattccgttc ccttaaagta tataatttta gacttttctt
    10681 tattgggcag tttatctccc ttattggcac atgggtgcaa caggttgcat caagctggct
    10741 tatatatcag cttacctcat catcgatgtg gttgggatta tctgcatttg cagcacaaat
    10801 acctatcttt gtcatgacac cactcacggg agttgttgcc gaccgtttca acagacatgc
    10861 tatctttatg attacgcaaa cgatgctatg catacaatcg cttatccttg gattgcttgt
    10921 tatattcgat tatattactc cagtcagcat tgtgttactc aacctgacga tggggtgtat
    10981 taatgccttt gatatgcctg tgcgacagtc ctttttattt gacatggttg ctgataaaca
    11041 gcttttaagc aatgctattg cgctcaattc agtagtattt aacagcacgc gccttatagg
    11101 cccgccgctg gcaggttttc tggttgcaca gtttggtgaa ggtttgtgct tccttatcaa
    11161 cacaacaaca tttgcaggga ttatactggc attattgctt atgaaggata tacattattc
    11221 taagattttg cataccagcg atttcaagga aaatataaaa gaaggttttg tatacgtaaa
    11281 aaatcattat cctatcaaaa gcgtgctcat tcttttaggg ctcatgagct taattggaat
    11341 gccgtttatg gtgctgatgc cggtagtagc agtggagata ttacatggtg attcgcatac
    11401 attgggattt ttgctgggta ctgctggatt tggggcgctt gttggtgcgc tgtttatggc
    11461 ttcacgcagc agtaccacgc ggtttgaaaa caaaatagcg gcaggatcac tattgctggg
    11521 gagcacactg gttctctttt ccttttcacg gaacctgtat tgttcattga ttttaatttt
    11581 tatcattggc ataagtttaa ttatacagat ggcatcctgc aatacattta ttcaatccat
    11641 ggttgatgac accatgcgtg ggagggtcat gagcctgtat ggcatgtcgc atgttggaat
    11701 ggctcctatc ggtaacctca ttgcaggagc tatcgcccag aaaataggcg ttgcccatac
    11761 attgttgttg tgcgggacgg gatgtgctat tgtagcattg tggttttata aaaacttaca
    11821 caaattaaaa aaacacatcg ttcgttatgt gtgatagttc atatatgaat ttgttttgct
    11881 gatcaatttt ctttgcagta tgcaattatt attgggagga aagccgtagc aacgttgcat
    11941 ataaatccat tccataaaaa ttaaaaatat tgacagaata aatataaaaa actattttca
    12001 tattgaagac gttatttttt tatactataa aatcaaattt caagttatga ataaagataa
    12061 attattggat aaatctatca acttatcttg taacaattgc atacaaaaaa ataatacatt
    12121 gaatggggat agttgggatt ttaaaaatgc gaatacaaag gaatatacac attgttttca
    12181 ttcttatcca gctatgatga tacctcaggt agcgcggcga ataattgaaa tttatgggaa
    12241 aaacgcaacg cttctttttg atccatattg tggcacaggt acttctttag ttgaagccaa
    12301 tttaaaaggg ataaatgcta taggcactga cctcaatcca ttagctcgat taattgcaca
    12361 agcaaaaact accaaaatag atagtaaagc attggataaa tatttgtatg attttacaaa
    12421 ttatttgttt tccaaaagct tcaaagagga agactatagt actataacgc tcccatgcat
    12481 aacaaatatt gatatatggt tttcaaagtc ggtgcaatat aagctagcaa taatattcaa
    12541 ttatattatt caaaaaatag acaatgtatt tatccaaaat ttttttaaag tagcttttag
    12601 tgaaactatt agagaaactt caaataccaa gaatggtgaa tttaaattag tacgagataa
    12661 aaaattaatt aaaactgaag atttagatgt ttttgggatt atattaggaa aattgtcaag
    12721 aaacaaggaa ggacttatta actttattaa ggtttgccct cagaatgtag tttcacacat
    12781 ttattcattt gatactgtgc tgtctattcc atatgattta gtgccacaag aatctgttga
    12841 tatagttgta acatcaccac cctatggaga ttcaagaact actgtagctt atgggcagtt
    12901 ttcaagattt gcaaatgaat ggttgggatt ccataatgca aataaaatcg ataatttgct
    12961 catgggtgga aggaaaaata atgtaaataa tttcaaattt aataacgatt tgttgaatga
    13021 tgttattacc gcaatatcaa ataaggataa caaacgagct atggaggttg tttcattttt
    13081 taaagattat gaatcatcta ttgttaatgt atctgctacg ataaagaaag gtggagttgt
    13141 ttgctatgtt gttggcaata ggacagtaaa aaatattaat attccaacca ataaaataac
    13201 tgcacaattg tttgagcaga ataattttgt acatatggaa accattgtta gaaatattcc
    13261 taataaaagg atgcctctga tgaatagccc aactaatatt cctggacaaa cagggcaaac
    13321 aatgaaaaat gaatacatcg ttatatgtaa aaagatataa agtatgcaac taaaaaaact
    13381 tgtgaaaaat ttagaagata taaagagtaa aggatttatt gagacaatta ggaaaggccc
    13441 aaccgctgtt ggttatttac ttgaaaaaga actagggctt agtgaaacca atataacaat
    13501 tccagatata ggaggtagga ttgaaattaa agcaacaaga aaaaacacca actctttaat
    13561 tactctcttt acattcaata gaggtgtatg gagaatgaag caaaaagacc ttataaataa
    13621 atatggctat atagatgcac aggggagaca ggcaatatat gctactgtta gtgcaaacgt
    13681 tcataatgct caaggattct ttttatcaat agataatgac aagcatcttg ttttgctcaa
    13741 aaatgcaaaa gaagatgaaa ccattgcaga atggagtacc tatgtcattg caggtaaatt
    13801 tatgactaaa ctagatagat tgctattgat attagcagat tctaaaatgg ttgatgagaa
    13861 agaatttttt cattataatg aagcttatat tttagaaaat ccaacacctg aaaaattttt
    13921 ggaaggattt aacagaaatg aattgatgat tgatttgcga atgcatttaa aagattatgg
    13981 tggtgtcagg aatcatggaa cggctttcag aatttctgaa aaaaatctta ccgacctata
    14041 cacaaagaaa agaagattga tataaaatat actataaaaa tagagcttga cctttttgcc
    14101 ctatttttac gttttatagt tattctgtat gagtgcgtta tagtgcacaa catatccagg
    14161 ctttttattt cactgtataa agctattctt aatgaggttg ttattatgga aggcatgcaa
    14221 cacattacat ggataccgtt tgatatcgct acccgctaca tggttgatgt ttttaccggg
    14281 ttaggcgttc cgcatgatga tgcgttaata tgtgcagatg ttttaattac tgctgacaaa
    14341 tttggaatcg attcgcatgg cgttaaccga ttgaaaccaa tatattatga ccggataaag
    14401 cagggcattc agaagcctgt tactcagtgt gctgttgtta aagaaggacc aactactgcc
    14461 gtgatagatg gcaatgatgg tatggggcag gttatagcct accgctcaat gcagatggct
    14521 attgataagg caaaaaaata tggtatgggt atggttaccg tacgcaattc cactcactat
    14581 ggcattgccg gatattatgc caaaatggct gctgacaatg ctatgatagg cataaccggt
    14641 accaatgcaa ggccttcggt ggcgccaaca tttggcgttg aaaacatgct gggtaccaac
    14701 cctcttacct ttggcatccc tgctgatgag aattttccgt ttttgcttga ttgcgccacc
    14761 agtgtaagtc aacgcggcaa aatcgaggtg tatgcccgcc ttgacaaacc tcttccccca
    14821 ggatgggtta tcgataacca tggcaattcc tgtactaacc ctcatcaggt acttgatggg
    14881 cttattaaag ggagcatggc tctgacccca ttgggcggta ttggcgagga tggcgcaggg
    14941 tataaagggt atggctattg tactgttgtt gagatactgt cggcggcact gcaggcgggg
    15001 agctatttaa aaatgctcag cggctttgac atgcagggta ataaagtgcc ataccattta
    15061 ggacatttct ttattgctat tgctattgag gcgtttaccg acattgcatc gttcaaatca
    15121 attaccggtg atatcctgcg tcagttgcgc aattcagcca gaatgccagg tcaaaagaaa
    15181 atctacacgg caggcgaaaa ggaatacctc acttacctgg agcgtaaaga taagggtgtg
    15241 ccggtcaata acgaagtaat gaaagagctt gtagccatgc gcgatgagct gaatataaca
    15301 ggatatgaac tgcttacctt gcatgcttca tgaacagtgt ggcgtgctat gatggcaaat
    15361 gaaaaccata gcaatgatga atttccccga gagataacgt ttaaggtgat ttttcacaac
    15421 acgctatttc ttaaagagat gctcatcaat atttgtgccg agaatactat tgatgcacac
    15481 attactgaac gtgaaagcaa gcaagggaaa tttatttcat ataccattac tgcgctgttt
    15541 gcccacgaag aagtgttaca tgcaatttgt agtcacataa cacggctgga tggcttcatc
    15601 accatgtttt agcactattg gttggcgata gttccggtaa tctgtgtaca ctcaagatta
    15661 tctgcatgta gtatatgagc tttgctgctt tccatgaaac ggtgtgcacc ctttactaca
    15721 accctgcata cagcaaattt taaccggcaa aaactgctgt atgaaaacat tgcaatacat
    15781 tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgtgat tatatgtcac
    15901 aattctctcc tctgtatacg tcacttcatc aaatactatt gcagtgaggt atttctatga
    15961 gcatccttac cacaattgaa tccaacctta aagacgcaat gaaaagcaaa aatgaactgt
    16021 taagcagtac cttgcgtatg atgaaatcgg acatactgta tgaaaagact aagagtacgc
    16081 aggagcttac cgatgaaaaa atcattgagg tgttaacacg cagcgccaaa agaagaaaag
    16141 aagcaatagc tgaatatgaa aaagccggca gagatgacct tgctcgtaaa gaaaagcagg
    16201 aacttgagat aatcatgcag tatctacctc agcagatgag tgctgatgag atagcctctc
    16261 atgttgatgc agtgattgct tcaatgggaa ctatcaccaa taaggatacc ggtaaggtca
    16321 tggggcaggt gatgaaagac ctgaaaggga aagccgatgg gcaggttgta aaacaaatag
    16381 ttgctcaaaa acttgaaaaa ttgtagattg caccatatat ttataacaaa tggctatacc
    16441 ccgcgatacg attgaacata tacggcagaa ggctgccata gaagatatca tacggcgtta
    16501 tgttcccact cttacaaaaa agggtaagaa ctatgttggt ctgtgccctt ttcataagga
    16561 aacaagccct tcatttactg tctcgcctga taagcagatg ttttattgct ttggctgcca
    16621 tgagggaggc aatgtattca ccttcatagc aaaggtagag catgtagatt ttgttcaaag
    16681 tgtaaagatt gttggcgaga ttgtcggcat tcctgttcgt aatgaggaaa agcaaaacga
    16741 tgaatataca acattactgg caataaatgc aatgatagca gagcgttacc gtcaggcgtt
    16801 gatgtcagcg gcaggaaagc gtgctctgga ctatctcttg catcgtgggg ttacccgaca
    16861 gggtattgac gaattccaat tgggatatgc acctgatgaa tggcagtatg caaccgatat
    16921 tttgcgacag aagaatgttc cgcttgatat agccgttaaa accggtaata tcaataaatc
    16981 ggtaaaggat tctacatcgc attattatga caggttccgg aacagggtaa tatttccaat
    17041 ctttgaccaa aaaaatgaaa taattggttt tggtggtaga attattgaat ctggcgagcc
    17101 aaaatatatg aattctcccg aatcactggt atttcagaag cggaatattc tgtatggatt
    17161 caacaaagca cggcagcaca ttgccgaatt gggcagggct attgttgttg aaggctatct
    17221 tgatgttatt ggagtatatc aggctgggat tcagaatgtt gttgcaccgc ttggcaccgc
    17281 ccttacgcag gagcagctac aactgctgtc gcgcctgagc aatgaggtaa ttattgtttt
    17341 tgatgccgat tcagcggggg taaaggcatc gttgcgttcg cttgacgttg cagcaaacct
    17401 taacataact ataaaaatag cgcagttgcc cgagatggac ccatttgatt ttgtcaacaa
    17461 acgtggtgca cgggaattta tggctgttgt tgatagtgct ctcaagccgg ttgattttcg
    17521 cattgagcgg attattgcag aaaataaagg aaaatcacag gttaatgtac tgaagcagat
    17581 ttttggcatt ttacgtacta tcccatttga ggttgaacgg cagcactatt ttaaaaagct
    17641 aagttcagtg tttcaggttg atgaaaaggc aatacgagct gattatgaac gttttacaaa
    17701 aggtaaaaca actaatgggg tgatagttac cgataataat actgttgatt tttatgcaaa
    17761 aagccagcgg gaattggtat tacttttatg caattatccc ctgcttattg aaaatgccat
    17821 tgttgattgc ccattacagg attttactga tactgtagca aagaatatct atcagacatt
    17881 gattgctttg tatgaagaca atgaatcttt ttcactggaa aagatgtttg atttttatcc
    17941 tgaaggtgaa gaaaaaaaat tgctgacaca gggcttttca aaacaatttt catttgaaga
    18001 tcctcatgtt gcatattcgg aaattatact aaatatgaag cttttcacaa tcgacaaaaa
    18061 aattaatgaa tttgcagaaa aaattgaaaa ggacaatcgt gagaatatac aatattatat
    18121 aaccgagatt gaagtattgc gacgtgaaaa agaaaagtta acttcgtaca tctacaataa
    18181 aggaagtatc aaacattaat gaaatagtag ttgtttataa catcaataca gggattgtat
    18241 gaattcttgt gattgttatc aatgattttg taagtaataa taattttaat gtaaagggta
    18301 gaattaatta tggcaaaagg cgatgtactt ttagagagca ttccggaggt taaaaaactt
    18361 gtggaattgg gtatatcaaa tggtgaaatt acctatgatg agctcaatga tattttaccc
    18421 gacaagatat taaattctga taagattgat gatattttta ttctattgaa ccaattgggc
    18481 attgatgttg ttgaagaacc aactcgacga aatgatgctg caccattaaa aaaagaaaaa
    18541 acgcattcca aaaaatcatc ttcttcatca tctgaaacag gcgtttcaga tgatccaata
    18601 cggttatacc tgaaagaaat agggaaggtg tcgcttttat cgggtgacca ggaggttaaa
    18661 ttagcaaaac gaatagaaga aggtgaaata ttaattgaaa atgctgttct ggactcaacg
    18721 gtgctgctca atgaacttgt taaaaatcac tcaaaagtaa aaagcggtaa gattaagtta
    18781 actgatattt tgcgtattaa caagctgtac tatttttctt cgtttgatat gcacgatctt
    18841 gaaaaacgat atgaaaataa tatgagcatt atcattgctg aagataaaaa gataatgcaa
    18901 agtcaagcca aacttaaaaa gttatctgaa gattcaaaaa aagcattaga gttgaaagaa
    18961 aagatgatgg catcccgcag tagtatcagg gaggcattga taaacttaca ggtgcatgaa
    19021 aatgagataa ataaaactgc acagcgaata ttatcaatgg ttgcccgtat aaaagaaacc
    19081 cgtcattttt ttaataatct ggaaaacagt tttaacaaaa ctgtcaaaga gctgaagcag
    19141 tttgggcgta aattagaaaa gaatgaagat gtaaaaaaga tattaaaaga gctgaagatt
    19201 aaaaataaag atgagctttt agagatagtt aaggatatca gaaacaatga gcggaagata
    19261 cgcaggattg agcaggaagc tggttcaacc tgtgacgaaa ttatggaatg gggcacacag
    19321 attgaaatag ggcatcggaa aatatcactg gcaaagaagg aacttatcaa tgctaatctg
    19381 agacttgttg tatcaatagc caaaaaatat gcaaatcgcg gcatgcactt ttttgacctt
    19441 atccaggaag gtaatattgg actgataaag gctgttgata aatttgaata tcgaaaagga
    19501 tataaatttt ccacctatgc aacatggtgg atacgtcagg caataacacg agctatatct
    19561 gatcaggcgc gtaccatccg tattccggta catatgatag agcaaattaa taaggttatg
    19621 cgtgaaacac ggttgtttca acaggagttt ggccgtgaac caagtccgga tgaattagcc
    19681 gatagattag gctggcatgt gagcaaggta aaaggtgtta aaaatgttgc ccgtgagcca
    19741 atatcgcttg aaacccccat tggcgaagaa gaagactcgc tactgggtga ttttatagaa
    19801 gataaagaag tggattcacc tgttaataca gcagcatatc gcttattgtc agaacagata
    19861 aatgctgttt taagcacatt accagcacgt gaacaaaagg ttatccgtat gcgttttggg
    19921 ctggatgatg gctactcgca tacgctggaa gaggttggtt atgtgtttaa agtaacccgt
    19981 gaacgtatac gccagataga agcaaaggct ttaaggaggt tgcgccatcc taccagagca
    20041 cgaaaattaa aagattacct tgactatatg taattagtct gtaaaatttt aatagtccaa
    20101 tagataatta atagatagaa gatggtatct tttatctatt aaattaaagg tacactaata
    20161 aatcattgta atgcccattg tatagtgtga ataattaata tgcatcatca ggaaaataca
    20221 aatttaatta aaactgttac catcgttgga gctggttcct ggggtacagc attagctaat
    20281 gttatagggc acaatcaccc tgaaatgact gtcaggatgt gggcgtatga aaaggatgtg
    20341 gtgcgcacca ttaataataa tcatatcaac actgcatatc ttcccggtat tatactggct
    20401 gattctataa ttgcgtatca tgatctcaaa gaatcagtga aacaaactga cgctattatc
    20461 attgccactc cttcaaaggt tgtttatgat atctgtgtaa aattacgacg tcatatacag
    20521 cctgatacct attgtggtta tctttcaaaa ggattttgta aaattggcag tgggatatat
    20581 acaatgtcgc aaacaataga acttgcgttg ccatttttaa tggatacagt ttgtgcagta
    20641 tatggaccca gccatgccga agaggtttgt aaagaatacc atactgcatt acacatagca
    20701 agcaaatcca atagctcaat taattttttt atgaacttac tatcaaatga gtacatagaa
    20761 tgtataccta taaacgatat cattggtatt gattgtggaa gcaccttgaa aaatcctgct
    20821 gcaattgctg cagggcttat cagcaacctg ccacaatgtg gtgacaacct catgggagct
    20881 ctaattgcac aggcgctgaa agagatggtg caactgggta aatgtttagg agggcaggag
    20941 gatacaataa ccggtatagc aggtctgggt gatctggttg ctactgcatt aagccagcat
    21001 agcaggaaca ggaggtttgg taaagaaata ggagagcaga taacacaaaa aggtacgaca
    21061 ttaagtttgt tcgatagatt tcttttacgg tttaaacccg acgatgttct gggaagaatg
    21121 ggtggaaggg tacattacct tgttgaagga gcatatgcta ttgaacccat tattgaattg
    21181 gctgatagat ataccatcac catgcctatt tatcgttctt tatatgaggt attgttaaat
    21241 aaaagagatc catggctttt aattgagact ataaaaaatc ccgcaaaata tgaaattctt
    21301 acccgcaggg cgcgaattaa agcaaaggaa agaaaaaaag gcatagagcg gatgagcggg
    21361 ctcatattca aaaatattgt cgttgaacga atgatcaaag aactatctgt ccctgaaaaa
    21421 aaaagagagg tgcttgatca aaaaaatgaa tatatacaat tgctgcacca gtataaacat
    21481 ctaaaaaaac ctaaaaaatt tttatatgaa ctatcgctct ataacaatct gaacgaaaaa
    21541 aattatgaag agcagcttaa aacaattata gaattttatt ataccagcat aagcgataga
    21601 tatgtatatt cattttcttt attaatgctt aaagcagcac ggctgttttt ttatatatat
    21661 ggattgctat accggcgtaa aatagtagaa ttttttgagg aaagaatttc tttaagtggt
    21721 gatattaaat atttaaaaaa aatagcaatg acagccaatc cggtctatat ttgtaacgcc
    21781 aaccagattg ctgattcaat ttttgttgtt ctggcgctca taaaatttat aactattcct
    21841 ttgccgcgat ttaatattaa cagccagtta ttaaaaaact ctttgttgcg ctaccttttc
    21901 agggtgagtg gtggttatat tgttgataaa acacggtatt cttctacact gtatcgtgag
    21961 acactgctca attacatttt aatctgtgtt gaacatggta ttaccatact gtattcaaat
    22021 gattcaagtg caccaaatga cgaaacaact gactccatat cacaaaacga tattattcag
    22081 gatgtactta ttgaaaggct atcaattctt ttacaacaaa catcagagga gattgctatt
    22141 atccctgttg aagttgccca taaatattat aa
//
LOCUS       DAOSOX010000018        21967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2368_length_21967_cov_9.355787, whole genome shotgun sequence.
ACCESSION   DAOSOX010000018 DAOSOX010000000
VERSION     DAOSOX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21967
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_21967_cov_9.355787"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2464)
                     /gene="cas3"
                     /locus_tag="PLX22_02520"
     CDS             complement(122..2464)
                     /gene="cas3"
                     /locus_tag="PLX22_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOT18809.1"
                     /translation="MFRWEIFSVMDSLNFETLKKQIFKKFQLNQYLAKSDKNYKTNTS
                     IEDHVKDLLYELDNFLSKYSDVIKTTDDMLLLRLAILFHDIGKINILFQKKLTENKKD
                     DVIPHNCLSPLFLKLVNKLEEDKLSLLTYIIFNHHKRGANYFSNDSYGINLLIKQIQE
                     NQKYFFNNFEFSEETLENYKVWLLNSLRNRKSKKYIILTGLLIRLDHAASGELEVEEE
                     PIKDDRKDILFSYFKRKNKTTKLRPFQERYGINEKKDYQCIVADTGLGKTGLSVLWSH
                     KKIFYILPNRASVNAMFNTLKEIYGEYNVGLLHSTALFNLIQDNEDDIAVLKEYDQTR
                     VLSKPVTVCTADQLFTAAFNMPGYEKIYATLSYSDVIIDEIQGFQPQQIIPMLKQIKE
                     TKELGANYLIITATLPSIVTNRLMEYGFDVIENDDCTTDPIKRHKIKIEKGKTIEDLS
                     EGIVDTAVSGKKVLVIVNTVKKAQEMYNKINNTTKEHRANFIDVNILHSRYIWKDRQV
                     KENAIIDECEQDNNFNYKKQNYSIWITTQLVEASLDIDFDYLFSEASTADSVIQRMGR
                     VWRHKNVDYNGDENIIIATAVENTVYEKILIEKTVEMIENSIKNNDNILISSEKRRIV
                     NELYSEKNLKQWRSKYIEEWNKFEEQINSGWNFILEENSQRAFRDVMSIDLIPAIYKE
                     IVESSYKKLLDLRNEKSLDGKVKRLQRLQILKEIQEYKVPVPLYLLNPNVSRKLINTS
                     QPIEWIDKGYGIGILHEYYKYDQILGLTCKGIKGEDEIII"
     gene            complement(2425..3057)
                     /gene="cas5"
                     /locus_tag="PLX22_02525"
     CDS             complement(2425..3057)
                     /gene="cas5"
                     /locus_tag="PLX22_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOT18810.1"
                     /translation="MLRLIIYQETAHFRIATIGNPYLTYLLPPPSTIYGFLRKITNYK
                     SINYQNTKLSIQGIYESISLEKERLTLETKREIKTNIIPIQKLHNSTWIIHIDSVYDR
                     EIKEGINDYVGILRLGRVEDLIIDFSLEEIMISDNDYKNIDVDYFIYKKWEQGKDFKG
                     QLFSMFIDSEVDEKLNIIGYKPVQLIYSSVYSSLDEIKCSDGKYLVSWIH"
     gene            complement(3077..4024)
                     /gene="cas7i"
                     /locus_tag="PLX22_02530"
     CDS             complement(3077..4024)
                     /gene="cas7i"
                     /locus_tag="PLX22_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HOT18811.1"
                     /translation="MKLTGVFLIETGVVNRGDGIGNIATVKKITTPQGIKVYFSPQSY
                     KRALWNSLQNNFGWSLPLVTKEGGVVQRTGTVIDSEEFDFGGTMVAKPMYQRNTTFYL
                     DNGISINNFTGDMEFMTNMGIAKLYGENEANIINRENFYGIYLLPFGIEIDRIGIQEL
                     NLDLTVSFESNDNLEQKIEKIYTALKLKEQIDDYKIKMWVNRIASIEIQNNKIIIILT
                     IDERKRRFQQLMEAMGELGRKIEGSERNLCPHFAFFSLTYATPKYLLFIKDKLIRKEK
                     LTEQDIQHYAEKDGKNIIIATHENYKEKIKEIIEAVFPN"
     gene            complement(4051..5694)
                     /locus_tag="PLX22_02535"
     CDS             complement(4051..5694)
                     /locus_tag="PLX22_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18812.1"
                     /translation="MNKEKITFTLTGNFQYDLGILGLKRVLDFFGIKYESDKKFYITL
                     QRDANTLLEDIIAKLIFDNGINYFWKKLLEDLKKKNKNNKDIQKKIEELGKVENISCE
                     CDLKENYKDKGLTLLIENISDVLFNILNDDMKNEIGVNYIKDLLWQKSVNLLNNILLN
                     FQADMNIKGEGTLRKAKNKLNDNIVKDSKCSFCNQKMGKRLTRDVFFFAPSQYNAFWF
                     SEPSLYVCPACLVSNFAITQAFTFLGNKLDAIVFYTPNLEDMEKMNVTLTSHFNDNLN
                     FGFFKIVKSLIEYEKEILKKEASVKELQILSFRLDSKNPLIEMFILSEGTIYNLIKIE
                     NQLNSLFDENNISKLFGYIREKDRYAKIDFSKELMKNLSQNQKIIYLVQKYSRYCIMS
                     ETFKQNKTEKPPVRNFTPWLFLNFLKIHFKLEGYMIDEFNKFQEIGQRMRQRVYMLLT
                     DNKTKSINWNTFDNKIISLSNSFLNTSKGSLQQFQELLSRVIISLGLNIDMSIIKIIN
                     KENFGEITTIIALALLVSQPAENRNREDDQMQDIKKEQI"
     gene            complement(5749..6129)
                     /locus_tag="PLX22_02540"
     CDS             complement(5749..6129)
                     /locus_tag="PLX22_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT18813.1"
                     /translation="MKEITKQWIKKAEEDYIVTSREIELDKPVCSAVCFHSQQAVEKY
                     IKAVLQENEIYFPKTHDLEKLMDLCKEYIPELEQLADGLLWLTSYAVEVRYPGITARK
                     KEAIKSYEIAKKVRDMVRRHLDVD"
     gene            complement(6126..6431)
                     /locus_tag="PLX22_02545"
     CDS             complement(6126..6431)
                     /locus_tag="PLX22_02545"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT18814.1"
                     /translation="MMIIDELANVIKKEFSVQKIILFGSHAYGQPHKDSDVDICVIME
                     TNLPFPEQAAIIRLKLREKIKEKPPIDLIVRTPDSIKEELKKNNFFIRDIIEKGKQL"
     gene            complement(6428..6817)
                     /locus_tag="PLX22_02550"
     CDS             complement(6428..6817)
                     /locus_tag="PLX22_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT18815.1"
                     /translation="MKEITKEWVNKAEKDFAVVLRLLENSDPVFDAVCFHSQQCVEKY
                     LKAVLQENEIYCEKTHDLWLIAQKCLPFISKLNTMKSDLIRLTAYAVEVRYPGFEADK
                     EAAENSLAICTEVRKLVRQYFGLEVAG"
     gene            complement(6814..7134)
                     /locus_tag="PLX22_02555"
     CDS             complement(6814..7134)
                     /locus_tag="PLX22_02555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT18816.1"
                     /translation="MNDELYQSLKEISHFIKEKFLAQKVILFGSYAYGNPTPDSDIDF
                     FVIMDTDKRFPEEAANIRIGIDEEFHIAKPIDILVRTPGYVQKRLGMGDFFMKDIIEK
                     GIEL"
     gene            complement(7131..7895)
                     /gene="cas6"
                     /locus_tag="PLX22_02560"
     CDS             complement(7131..7895)
                     /gene="cas6"
                     /locus_tag="PLX22_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOT18817.1"
                     /translation="MRFVVHMHFDNGNDILLPPWYRIGFASMIKEALRQDATSKMYEL
                     YYGNAKKNRPKPFTFAVKLNVADVEKSNVNYLRLGNPFISFYISSNDYEFIMTVYNGL
                     LKLKQYAIYNNTIIIDRFNLLPEKTFPESKAVFDIFSPIVVRKVDENKKGTGYVNVND
                     STYKQMLFYSIRSQCKFLGDSYLLNDDDVAINTSSAFTVKIPHYNKRNPDKPEVIAAT
                     DGCIAIQAPNEVLQLIYDIGLGARRSQGFGMLEVAG"
     gene            complement(8104..8472)
                     /locus_tag="PLX22_02565"
     CDS             complement(8104..8472)
                     /locus_tag="PLX22_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18818.1"
                     /translation="MLLPLFDKDCEHAGWLDPGKYIYDADMNFIAFIINSNAWSVETG
                     NWLGPVQGLLCYDTNGKLMFWNPKEKITGTVNAPRPSRQILATKPQTPAKPAEPAKPA
                     PPKCNANVWSDLSLWGWLSQ"
     gene            8931..9383
                     /locus_tag="PLX22_02570"
     CDS             8931..9383
                     /locus_tag="PLX22_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT18819.1"
                     /translation="MKWGLTKRDNTALNPFEEFERDVKKTFENFFSLEPANLFDSAWI
                     PTVDVKEDEKSIHVRAEIPGIDEKDINVALEDNILTISGEKKEERKEENKRYVLSESR
                     YGSFKRCITLPGEVKADSAKAKFKNGVLTIDFEKKEVSQPKRITINVK"
     gene            9395..9805
                     /locus_tag="PLX22_02575"
     CDS             9395..9805
                     /locus_tag="PLX22_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT18820.1"
                     /translation="MFGTYDLFDDVFALRNLVDRFFDEAAFSRQRVDFPYVNIYEKDD
                     EILVRAIAPGVKAEDVDVQLIDNTLTIEMTCKEDYANQPYLRKEREFGTFRKSIQLPY
                     QVDPNKVDASLNNGILTIKLSKHEAVKPRKIMIK"
     gene            9823..10203
                     /locus_tag="PLX22_02580"
     CDS             9823..10203
                     /locus_tag="PLX22_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT18821.1"
                     /translation="MATQLAQKPEQNVKKHDYVITPPVDIYETDNEYVLKADMPGVTK
                     DNVNITFNNNELEITGKVDEQYTSNENMTYREYTLFNYYRKFVVSNAINPEGISASLE
                     NGVLTVTLPKREEAKPKKIEIKAE"
     gene            complement(10562..11008)
                     /locus_tag="PLX22_02585"
     CDS             complement(10562..11008)
                     /locus_tag="PLX22_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18822.1"
                     /translation="MKKHTIVTMIALSIVLMLLACVGTSEITEKPAAPAFKYYGLDGL
                     YLTTDEKQMNGVAKDHAKLRVGEFVVVYARGMSTVDTGAQWFKLPSSVAVTWKADSEL
                     QVAPASGHTVTVRVVKPIAVTSYVTAVATAKNGKKIEALFTVEGKK"
     gene            complement(11412..11825)
                     /locus_tag="PLX22_02590"
     CDS             complement(11412..11825)
                     /locus_tag="PLX22_02590"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HOT18823.1"
                     /translation="MVVNIKKSGLLYVIVLIMVAGCTKDMKEPCTTINAVHYKGEEIV
                     INNGGCISFELESQLSTGHRWFFKIEQNAVVLVNSDYKVETMQQQVVGGIDKELFTFK
                     AVKPGTATIVFEYIRAFDKNKKPVKSKEYKVTIVE"
     gene            complement(11849..13000)
                     /locus_tag="PLX22_02595"
     CDS             complement(11849..13000)
                     /locus_tag="PLX22_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOT18824.1"
                     /translation="MKKHIAASITVCMLIAPVAFSHAQMKSSFKASVEAQKQRDAKSI
                     AEVKQQLAIIKKDIKERNLKFKVDMTEALKRQIDEITGAIPPEDLEKEAEIQTNSGLQ
                     LFLEFLQRLSESKAKKQKEATLPPKKEPVKPEEAKKEEPKAVEPEKPRETLPESFMLA
                     DPSKPALDWFALGMMTPIKDQGLCGSCWSFTAMGVVEGCYKIKAKDEIDLSEQYCIDC
                     ARDKYGRDAGSCNGGWYGNVFDFLMNNYPVEEKVAPYRGYEYGCTPKKQFPIKMVAWG
                     YVRADAGIPTVEQMKKALCTYGPIAATVKVTPAFQAYVGGIFDEFTPVSSDKDVNHGI
                     IIVGWDDSKHAYRIKNSWGQRWGEKGYMWIQYGCNNVGYGAAWVVLDKE"
     gene            13401..14243
                     /locus_tag="PLX22_02600"
     CDS             13401..14243
                     /locus_tag="PLX22_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18825.1"
                     /translation="MKSIRFLFTVTITVFVLAAVSGIVIAQQQAAPKEPQKEGIKEEV
                     TGWRIKDFKPYIKAIEELEKLSKEYSDNLLKLAIDEYSTGIDILEDMENEVNKLVETN
                     QKNKNLNEQWYWQEIDRREQEKRQISMMKFEAKSKAITYFTRAINHMDEITFEEVRKD
                     PKFVNFQIGLYKAYVSTQYDLYNLKPCIPILERYIAINDQTKNDVWAYKYLASCYGYM
                     ESVLVKYKQGTEQEQLQYKQKKNIYMLKAAELQYGIESPQYKHLREIVSQDEKKSEKI
                     NEFK"
     gene            complement(14313..15317)
                     /locus_tag="PLX22_02605"
     CDS             complement(14313..15317)
                     /locus_tag="PLX22_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18826.1"
                     /translation="MILKTGCSTFNCIFWMLLFIFHFCFCLQGNACEAFALHNGVIYL
                     DSNTSLPVNGTVLDFTAINNRAILYVARSEKEVTAGAYLLDEKKSIVCVVASINESSE
                     VKRIAFDPPVVYISIHDSTNCYLYRADVNIGIVQQKEAITDFVLLDGGLAVLTNDNYL
                     EYNDGKLPLYFLSRPYFKRAIDSRIVLVADTDATEVIDVVAQKPVYRYASNVQYVSSE
                     DHTVEIALYDMPSEEKAGRVFYKIMCDGVDYTRTEPGLSINSTAIDLNLTACQYHEII
                     IERWRLDETENKYIRDNNIRQPKPLTVYVYPGRVIVFTMIFNGTEYQVHSHFKVESQK
                     "
     gene            complement(15286..16995)
                     /locus_tag="PLX22_02610"
     CDS             complement(15286..16995)
                     /locus_tag="PLX22_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOT18827.1"
                     /translation="MRLAEPSIEHVQSKIPTDLEHLRKLFIMPDSPDRFMQFGYELLD
                     LIHSFFKEKGGIHSEISLPQLACLFNAIDIPKEPKLLKDILAEVKQKVIKHSVKVGNP
                     YYIGHMTSAIPYFMILLEMIIAALNQNQVKIETAKASTFVEREFISWIHRLIFCKSAS
                     FYKRNIQNHRIALGNVTLDGTLANLTAMLVARNKAFPADERFPGIRKAGVAETFRYYA
                     IHKAVFIVSQRGHYSIDKVARITGIGDNNVIKIPVDSNNKIDINALKKVLKEISEYNS
                     SHHDTIKVISLIGIAGTTETGNIDDLMALRKIADEYNIFFHVDAAWGGSVLLVNKYRD
                     LFRGIEKADSVTFDAHKLLYSPLSMGLILFKNPYDLLNIMHTSDYIIRPDSVDQGRFT
                     VEGSRPFSCLKPWVTLKIFGTTGFEVLFDHAFMLTSTLKDIINKHENFELLNSPELFI
                     TNYRFVPEGVQSKINRIYEQLSNADQQKATRLQKRLKKLNDGINEMNIELHRAIRQED
                     NSFVSRTMLESTRYRGQKIVVLRAITINPLTTPAILQEIIDEHNKLGLTIYHDFKNRL
                     FHI"
     gene            17524..18255
                     /locus_tag="PLX22_02615"
     CDS             17524..18255
                     /locus_tag="PLX22_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT18828.1"
                     /translation="MAILKLENVSLSLNNKPILDSISVDFWEGYIHAIVGPNGAGKST
                     LSYVIMGLNDYRTISGTLYFKNKPINELSVYQRARLGISLAWQEPARFEGLTVYQYLQ
                     ASKKDNGLSIEEAMDVIGLPAQEYLPRACDKTLSGGERKRIELASLLVMQPEVVLLDE
                     PDSGIDVEALERIFDAMAILKQRGSTIITITHSMAVLQKAEHAFLMCHGNIIDKGGID
                     KISGYFENKCIPCDHKNIPISGEAR"
     gene            18255..19199
                     /locus_tag="PLX22_02620"
     CDS             18255..19199
                     /locus_tag="PLX22_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOT18829.1"
                     /translation="MDDRRAVAQTLYKTMGLTDAALNDPDIAHLVINSNMVVASHLVE
                     GLVVEPAETQNGVRVTMRVLAGHIIQKPVHLCFGMLPEDGLQEIIMDVTIEEHASVSI
                     LAHCTFPNAKNITHKMEAVITIKEGASYSYLERHVHAASGFITVLPHAIVTVEKNATF
                     KTDFELLRGKAGIIDIDYETTCYEHSHLEMNAKVNGIQDDAITIRETGHLNGEGAVGV
                     LTTRIAVRDTAQATVYNTLTAHAAYARGHVDCKEIIQDRATAKAIPVVEVYHPKAHVT
                     HEAAIGSVDDKQLQTLMARGLSEDEAVDLIIQGLLSGS"
     gene            complement(19204..19650)
                     /locus_tag="PLX22_02625"
     CDS             complement(19204..19650)
                     /locus_tag="PLX22_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18830.1"
                     /translation="MTFDDTKIYAYFTIAMFIVALLFGLPGLYSTFITEPAIESLIYH
                     DNPSSQQLKQAYIMLRKPHIFAGYNRYDEVGAGIEYILKDFDLRVAEKKDFDVNDTMY
                     LELLLERRKQGSDLSIKTMVFFVLLSLLGSIALVIEKTSKNRMKER"
     gene            complement(19647..20108)
                     /locus_tag="PLX22_02630"
     CDS             complement(19647..20108)
                     /locus_tag="PLX22_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18831.1"
                     /translation="MKKILIICVGVVCLSCTRYVELGDVEFTVNVTFEKNVPVFASTY
                     TIYINNKSVDTVITSYSAVVSILDEEQKAVKQCTVTAEQILPKQQKQLFVQQKDSEDT
                     IKVLLDALEISVDELIKQREAALVLPARMVRVDNVTYKTVDIVTYLKEVEK"
     gene            complement(20370..20588)
                     /locus_tag="PLX22_02635"
     CDS             complement(20370..20588)
                     /locus_tag="PLX22_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18832.1"
                     /translation="MRDDLASNFKAELIWKSGEIIKKQLNEKTDKDHNNKFTFEIGSI
                     ENSAINKHASDTLEGTLNAFNARRLKYI"
BASE COUNT         6845 a         4128 c         3347 g         7647 t
ORIGIN      
        1 attgataaga taatcaatat caatgttaag tttacgtggc atgcgatagt tcctctacta
       61 tactagttgt aataattggg atcaaaacat taagcatagt tttgctaaaa attgaagata
      121 cctaaataat aatttcatcc tcacccttta tgcctttaca tgttaaacct aaaatttgat
      181 catacttata atactcatgc aaaataccta ttccatatcc tttatcaatc cattcaattg
      241 gctgggaagt attaataagt tttctactaa cattaggatt tagtaaatat agtggaacag
      301 gaaccttata ttcctgtatt tcttttaaaa tttgtaatct ttgcaatctt ttgacttttc
      361 cgtctaaact cttttcattt cttaaatcta aaagcttttt gtaactactt tcaacaattt
      421 ctttataaat tgcaggtatc agatcaatac tcattacatc tctaaatgct ctttgcgaat
      481 tctcttctaa aataaaattc caaccactgt ttatttgttc ttcaaattta ttccactcct
      541 ctatatactt tgagcgccat tgcttaagat ttttttctga atataattcg tttactattc
      601 ttcttttttc tgatgaaatt aaaatattat cattattttt tatgctattt tcaatcattt
      661 caactgtctt ttctattagt attttctcat aaaccgtatt ttctacagca gtagctataa
      721 ttatattttc gtccccatta taatcaacat ttttatgtct ccagactctt cccatccttt
      781 gaataacaga atctgctgtg gatgcttctg aaaataaata atcaaaatca atatccagag
      841 aagcttcaac taattgtgta gttatccata tagaataatt ttgtttttta tagttaaaat
      901 tattatcctg ttcgcattca tcaataattg cattctcttt tacctgtcta tctttccata
      961 tatatctgct atggagaatg ttcacatcaa taaaattggc tctatgttct ttggttgtgt
     1021 tatttatttt gttatacatc tcctgagcct ttttaactgt attgacaatt accaaaactt
     1081 ttttacctga tacagctgta tcaacgatac cttcactcaa gtcttctata gttttacctt
     1141 tttctatttt aatcttgtgc cgttttatag gatcggttgt acaatcatcg ttttctatta
     1201 catcaaatcc atattccatt agtctatttg tcacaataga tggcaaagta gctgtaataa
     1261 taaggtaatt agcaccaagc tctttagttt ccttaatttg ttttaacatt ggaataatct
     1321 gttgtggttg aaaaccttgg atctcatcta ttataacatc agaataagat aaagttgcat
     1381 atatcttttc gtatcctggc atattaaaag cagcagtaaa taattggtct gcagtgcata
     1441 ctgttactgg ttttgatagc acacgagtct gatcatattc ttttaatact gcaatgtcat
     1501 cttcattgtc ttgaattaaa ttaaacaatg ccgtagaatg aagtagtcca acgttgtatt
     1561 caccatatat ttcttttaaa gtattaaaca tagcattaac agatgctcta tttggtaaaa
     1621 tataaaatat ttttttatgg ctccacaata cggaaagccc tgttttccca agccctgtat
     1681 ctgctactat acattgataa tctttcttct catttattcc atatctttct tgaaatggtc
     1741 taagttttgt tgtcttattt tttcttttaa aatacgaaaa aagaatatct ttcctatcat
     1801 ctttgattgg ttcttcttcc acctctaatt caccggaagc agcatggtca aggcgaataa
     1861 gaagaccagt taatattatg tatttttttg actttctatt tcttagggaa tttaaaagcc
     1921 aaactttgta attttctaat gtttcttcac taaattcaaa attattgaaa aagtatttct
     1981 gattttcttg tatttgttta atcaaaagat ttataccata actatcattt gaaaaataat
     2041 ttgcacctct cttgtggtgg ttaaatatta tataggttaa caaagaaagt ttatcctctt
     2101 ctaatttgtt aaccaacttt agaaacaatg gtgataaaca attatggggt attacatcat
     2161 cttttttatt ttcagttaat tttttttgaa acaatatgtt tatttttcca atatcatgaa
     2221 acaaaatggc taaccttaat aacaacatat catcagtagt ttttataaca tctgaatatt
     2281 ttgataaaaa gttatctaac tcataaagca aatctttaac atgatcttct atactagtat
     2341 tagttttata atttttatca gacttagcta aatattgatt tagctgaaat tttttaaata
     2401 tttgcttttt tagagtttca aaatttaatg aatccatgac actaaatatt tcccatctga
     2461 acattttatt tcatccagag aactataaac agaagaatat attaattgaa caggtttata
     2521 tccaattata tttaatttct catctacttc actatctata aacatactaa aaagttgacc
     2581 tttaaaatct ttcccctgct cccatttttt atatataaaa taatctacat caatattttt
     2641 gtaatcgtta tctgaaatca tgatttcttc taaagagaaa tctataatta aatcctcaac
     2701 ccgacctaat cgtaaaatac ctacataatc atttattcct tcttttattt ctctatcata
     2761 tactgaatca atatgaatta tccatgttga attatgtaat ttttgaattg gtataatatt
     2821 tgtttttatt tctcttttag tttcaagagt taatctttcc ttttctaagg aaatactttc
     2881 atatattcct tgaattgata gttttgtgtt ttgataattt atgcttttat agttagttat
     2941 ttttctcaaa aagccataaa tagtagacgg tggtggaagt aaatacgtca aataaggatt
     3001 accaatagta gcgatacgga aatgtgctgt ttcttgataa ataattaatc tcagcaattt
     3061 tgacctcctc tatcaattaa tttggaaaaa ctgcctcaat tatttccttt attttttcct
     3121 tgtaattttc atgagtagca ataataatgt ttttcccatc tttttccgca taatgttgaa
     3181 tatcttgttc tgttaatttt tctttcctta ttaacttatc tttgataaac aacaaatact
     3241 ttggagtggc ataagttaac gaaaaaaacg cgaaatgagg gcataaattt ctttcactcc
     3301 cttctatttt cctaccaagt tcacccatcg cttccattaa ttgttggaac ctcctttttc
     3361 tttcatctat agtaagaata ataattattt tattgttctg aatttcaatt gaagctattc
     3421 tgtttaccca catttttatt ttatagtcat caatttgttc ctttaatttt aaagcagtat
     3481 aaattttttc tattttttgt tccagattgt cgtttgattc aaaactaacc gttaaatcta
     3541 aattaagttc ctgaattcca attctatcta tttctatacc gaatggcaaa aggtaaatac
     3601 cataaaaatt ctcacggttt ataatgttag cttcattttc tccataaagt ttagctatac
     3661 ccatattagt cataaattcc atatcaccag tgaaattatt gattgatatt ccattatcta
     3721 aataaaatgt tgtatttctc tggtacatag gctttgcaac cattgtcccg ccaaaatcga
     3781 attcttcact atcaatcacg gtacctgttc tttgcacaac accaccttct ttagtaacta
     3841 aaggcaaaga ccatccaaaa ttattttgta aactgttcca taaggctctt ttatacgatt
     3901 gtggtgaaaa ataaacttta ataccttgag gtgtagttat tttttttaca gttgcaatat
     3961 ttccaattcc atccccccta tttacaactc ctgtctcaat taaaaaaact cctgttaatt
     4021 tcataataat cctcctcaat attttataat ttatatctgt tcttttttaa tatcttgcat
     4081 ttgatcatct tccctattac ggttttcagc aggttgtgat actagtagtg ctaatgcaat
     4141 tattgttgta atttcaccaa aattttcttt attgattatt tttatgatac tcatatcgat
     4201 atttaatcct aaactaataa ttacccttga tagcaattcc tggaactgtt gcaatgatcc
     4261 tttagaagta tttaaaaaag agtttgagag tgaaataatt ttgttgtcaa aagtattcca
     4321 gttaatagat ttagtcttat tatcagtaag aagcatatat actctctgtc tcatcctttg
     4381 ccctatttct tgaaatttat taaattcatc aatcatataa ccctccaatt taaaatgtat
     4441 tttcaaaaaa ttaagaaata accatggcgt aaaatttctg acaggtggtt tctcagtttt
     4501 attttgttta aaagtctccg acattataca atatcttgaa tatttttgaa ctaaatatat
     4561 aattttttga ttttgtgaaa gattcttcat aagttctttc gaaaaatcaa ttttagcgta
     4621 tctatccttt tctctgatat aaccaaataa tttactaata ttattttcat caaacaatga
     4681 attaagttga ttttctatct ttatcaaatt ataaattgta ccttctgata gtataaacat
     4741 ttctattaat ggatttttag aatctaatcg aaaactcaaa atctgaagct ctttaacaga
     4801 agcttccttc ttcaggatct ctttttcata ttcaatcaaa gatttaacaa ttttaaaaaa
     4861 accaaaatta agattatcat taaaatgtga tgttaaagta acattcattt tttccatatc
     4921 ttctaagttt ggagtataaa aaactatagc atccaattta tttcctaaaa aagtaaaagc
     4981 ttgagttata gcaaaattag aaaccaaaca tgcagggcaa acgtataaac tcggttcact
     5041 aaaccagaac gcgttgtatt gagatggtgc aaagaaaaac acatctctcg ttaatctttt
     5101 tcccattttc tgattacaaa aggaacattt agaatcttta acaatattat catttaattt
     5161 attttttgct tttcttaaag ttccttcccc ttttatattc atatcagctt gaaaatttag
     5221 tagaatatta tttaataaat taacactttt ttgccataat aaatctttga tataatttac
     5281 tcctatttca tttttcatat catcattcaa aatattaaac agtacatcag atatattttc
     5341 tataagcagg gttaaaccct tgtctttata gttttctttt aagtcacatt cacaagatat
     5401 attctctact tttcccaatt cttcaatttt tttttgaata tctttattat tcttattctt
     5461 tttcttcaaa tcttccaata gctttttcca aaaataattt ataccattgt caaatataag
     5521 ctttgcaatt atatcttcaa ggagtgtatt agcatctctt tgtaaagtaa tataaaattt
     5581 tttgtcactt tcatatttta ttccaaaaaa atctaatact ctttttaagc caagtatacc
     5641 caaatcatat tgaaaatttc ctgttagtgt aaaagtaatt ttttctttat tcatcccact
     5701 acccccgcgt tgtatagcca gtttgatgga taaagtataa tttcattatt aatcaacatc
     5761 cagatgcctc cttaccatat ctcgtacctt tttagctatt tcatatgatt taatggcttc
     5821 ttttttcctg gctgttatac caggatacct tacttctact gcatagcttg ttaaccatag
     5881 taatccatct gctaattgtt caagctctgg aatatattct ttgcataaat ccattaactt
     5941 ttctaaatca tgggtttttg gaaaatatat ttcattttcc tgaagaacag ctttaatgta
     6001 tttttctaca gcttgctgag agtgaaaaca tactgcacta cataccggtt tatccagttc
     6061 tatctctctg ctggttacta tataatcttc ttcagccttt tttatccatt gttttgttat
     6121 ttctttcaca attgtttccc tttctcaata atatcccgta taaaaaaatt gtttttcttt
     6181 agttcttctt taatagaatc aggggttctt acaataaggt ctataggggg cttttctttt
     6241 atcttttctc gtaatttcag gcgtataatt gctgcttgct ctggaaatgg taaattggtt
     6301 tccataataa cacatatatc cacatcgcta tccttgtgag gctgcccata ggcatgtgaa
     6361 ccaaaaagaa ttatcttttg tacactaaat tcttttttta ttacatttgc taattcatca
     6421 atgatcatca tcccgctacc tccagcccaa aatattgcct tacaagcttt cgtacttctg
     6481 tacatatagc cagcgagttt tccgccgctt ctttatcggc ttcaaaacct gggtatctaa
     6541 cttctacggc ataggcggta aggcgtatca aatctgattt cattgtattt agtttagaaa
     6601 taaaaggcaa acatttctgt gcaatcagcc ataaatcatg tgtcttttca caatagattt
     6661 cattttcctg taaaactgct ttaaggtatt tttcaacaca ttgttgggag tggaaacata
     6721 cagcatcaaa aactggatcg gaattttcaa gcagtctcaa aacaacagca aaatcttttt
     6781 cggctttatt tacccactct ttcgttattt ctttcataac tctattcctt tttcaataat
     6841 atccttcata aaaaaatcac ccatacccag tcttttttga acataaccag gggttcttac
     6901 caggatatct attggttttg ctatatggaa ttcctcatct attccaattc taatatttgc
     6961 agcttcttca ggaaatcttt tgtcagtatc cattataaca aaaaagtcta tatcactatc
     7021 aggtgttgga ttgccatagg cgtaagaacc aaaaagaatc accttttgtg ctaaaaattt
     7081 ttcttttata aaatgggaaa tttctttcaa actctggtac aattcatcat tcatcccgct
     7141 acctccagca taccaaatcc ctggcttctt cgtgctccca gccctatatc gtatataagc
     7201 tgcaacacct cgtttggtgc ctgtattgca atgcacccat cggttgctgc tataacctcg
     7261 ggtttatcag ggtttctttt gttataatgc ggtatcttta cggtaaatgc actgctggta
     7321 ttgattgcta catcatcatc gtttaacagg taactatcgc ccaaaaattt gcattgactc
     7381 cttatgctgt aaaaaagcat ctgtttatag gtactatcgt taacattgac ataccctgtt
     7441 ccttttttgt tttcatcaac ctttcttacc acaataggcg aaaaaatatc aaacactgct
     7501 tttgactctg gaaatgtttt ttcaggtaaa agattaaacc tatctatgat gatggtattg
     7561 ttatagattg catactgttt aagttttaat aatccattgt agacagtcat tataaattca
     7621 taatcgttgc ttgaaatata aaaactaata aacggattac caagtctaag atagttaaca
     7681 ttgctttttt caacatcagc aacattgagc tttactgcaa aggtaaaagg ctttggtctg
     7741 ttttttttgg cattaccgta atagagctca tacattttac tggttgcatc ctgccgcaat
     7801 gcttctttaa tcattgatgc aaagcctatc ctgtaccatg gcggcaacag gatatcatta
     7861 ccgttatcaa aatgcatatg aacaacaaaa cgcatagaac ctcctcttta ttatatatga
     7921 tgttcatcag gcgttattga tcggaatttt ttaactttct cccatattat ttatagtata
     7981 tactatactc attacgtggg acatatagtg tccctgtaat aatttttttc acaatgcagt
     8041 aaaaattttt acaggctata gttccgtccc acaacacaaa ataaacaata gatgttatgc
     8101 gtgttactgc gaaagccagc cccaaagtga taagtcagac catacattag cattacactt
     8161 tggcggtgcc ggttttgcag gttcagccgg tttggctggc gtttgtggtt tggtggcaag
     8221 aatctgtcgg cttggacggg gagcgttaac cgttccagtt attttttctt ttggattcca
     8281 gaacatgagt tttccgtttg tatcgtagca taaaagcccc tgcaccggtc ccagccaatt
     8341 gccagtttca accgaccatg cattgctatt aataataaat gcaataaaat tcatatctgc
     8401 atcataaata tatttaccgg ggtcaagcca tccagcatgt tcacaatctt tgtcaaataa
     8461 cggtaacagc attgtgcaaa cctccttata ctattttata tggtgacatg caggtatttt
     8521 tcataccagg aaaatccttt ataaagacaa tactatatat aagaattttt caacaaaaaa
     8581 attagttaac catcaactat ttgtagaata aatattcatt agtatagata catggtgtaa
     8641 taacatatgc acccgttctg ctgtaatagt atctttttca atgtgttagt tcaatacctc
     8701 agtgattatt atactatgtc atcatgccac ctctggtatg attctctgtg ttttaacgca
     8761 aattggtgaa aaatgtaata gatttctggc aatattttat cgtttagtga aaatattttt
     8821 ttacacccca cttgacgatt ttccaaatca gtattaattt atatacagaa attagcactc
     8881 actcatatag agtgctaaca acaaatcctg aagttcatag gaggtgtgct atgaaatggg
     8941 gtttaacaaa acgagataac accgctctca atccatttga agaatttgag cgtgatgtca
     9001 aaaaaacctt tgaaaacttc ttttcattag aacccgctaa tctttttgat tctgcatgga
     9061 tacccacggt agatgttaaa gaagatgaaa agtctatcca tgtgcgcgca gaaattccgg
     9121 gaattgatga aaaggatatc aacgttgcat tggaagacaa tatccttacc atcagcgggg
     9181 agaagaaaga ggagcgtaag gaagagaata agcgttatgt tctttctgaa agtcgatatg
     9241 gttcattcaa acgctgcatt actcttcctg gcgaggtaaa agctgacagc gcaaaagcca
     9301 aatttaaaaa cggtgtatta accattgact ttgaaaagaa ggaagtttcg caaccaaaac
     9361 gaataaccat taatgttaaa taaggaggca ggctatgttt ggaacatacg atctttttga
     9421 tgatgtattt gcattaagga atctggttga ccgcttcttt gatgaagctg cattttcacg
     9481 gcagcgtgtg gactttcctt atgtaaatat ctatgaaaag gacgatgaaa tacttgtcag
     9541 ggcgatagct ccgggagtaa aggctgagga tgtagatgtt caactaatag ataatacact
     9601 taccattgaa atgacctgta aggaagatta tgccaaccag ccatatctac gcaaagagcg
     9661 agagtttggc actttccgta agtccataca actgccctat caggttgacc ctaacaaggt
     9721 agatgcgtca ttgaacaatg gcatattgac aatcaagctg tccaagcacg aagcggtaaa
     9781 accccgtaaa ataatgatta agtaataaag gaggtgaaca gtatggcaac tcaattagca
     9841 caaaagcccg aacagaatgt aaaaaagcat gattatgtga taacaccacc ggttgatatc
     9901 tatgaaaccg ataatgaata tgtattgaaa gccgacatgc ccggggttac aaaggataat
     9961 gtaaatatta cctttaacaa caacgagctt gaaataaccg gaaaagttga tgagcagtac
    10021 accagcaatg aaaacatgac gtatcgtgaa tatactctat tcaactatta cagaaagttt
    10081 gttgtatcta atgccataaa tcctgaagga ataagtgctt cacttgagaa tggagtactt
    10141 accgttacat taccaaagcg tgaagaagca aaacctaaaa agattgagat aaaagctgag
    10201 taaaaagtgt atactttacc cgtcgccggt gcgacgggta attgttttta aagctgagta
    10261 aaaaggtttt ttacttattt tctcaccagt gatacgatga attattttaa agctgaatac
    10321 ataggtttat tacctgtttt cccgacagta atacgatgcc atttttttaa cgctgtataa
    10381 aaaggttaat tacttatttt cccgacagcg atatgataga atgttcttta agctgcatac
    10441 ataagtttat cacttatttt cacgccagtg atacaatgga atgttcttta agctcataca
    10501 tggattttat tacctgtttt tccactgaga cgaggggaat gttttccatg atttgtatat
    10561 tctacttttt cccttctact gtaaaaagtg cctctatctt tttcccattt tttgctgttg
    10621 ctaccgctgt aacataagag gtaacagcaa taggttttac caccctcaca gtaactgtat
    10681 gtcctgatgc aggtgcaacc tgcaattcac tatcagcttt ccatgtaaca gcaacacttg
    10741 atggcagctt aaaccattga gcacctgtat ctacagtgct catgcctcgg gcatatacaa
    10801 caacaaactc cccaactcta agttttgcat gatcttttgc aactccgttc atctgctttt
    10861 catcggttgt caggtataat ccatcgagtc catagtattt aaatgctgga gctgctggtt
    10921 tttctgttat ttcagatgtt ccaacacagg ctaataacat gagaacaata gataacgcta
    10981 tcattgttac aatagtatgt tttttcatac ttaccccctt atataattta ttagaatatt
    11041 agtattaata tttttttata atgttacctt tgatttacca tattattttt gcattaaccc
    11101 acattgtcat agtataaagt ttataagtat aacaacggac agcttttaat cagtatataa
    11161 tctattatga tatcgtatta tttaattttc aaaagcatgg tatacaaaat acaaaaacta
    11221 acaagaaaaa atattaaaaa atttctttac gataatgcgc cagcatccaa agacactctg
    11281 caccactgtc tggctgttta ttgctgtgat gacatactgt cattctgaag gagcgccagt
    11341 ggctgaagat cctattattt tcttcaacgt ctggattctt tggctacacc tcagaataac
    11401 aaacaatact attactcaac tatggtaacc ttatattctt tgctcttgac cggtttttta
    11461 tttttatcaa atgcccttat gtactcaaat actatcgtag cagtacccgg cttaaccgcc
    11521 ttaaaggtaa aaagctcctt atctataccg ccaacaactt gctgttgcat tgtttctacc
    11581 ttataatcac tattgacaag cacaacagca ttttgctcaa ttttaaaaaa ccatcggtga
    11641 ccggtgctaa gctgggattc cagctcaaat gaaatacagc cgccattgtt aataactatc
    11701 tcctcacctt tgtaatgtac agcatttatc gttgtgcatg gttccttcat atcttttgta
    11761 caaccagcaa ccattatcaa tacaataaca tatagtaatc ctgatttctt tatattaaca
    11821 accattgcag tgtcccccaa aaataaaatt attctttatc cagtacaacc caggctgcac
    11881 cataaccaac attattacac ccatattgaa tccacatata acccttttca ccccagcgct
    11941 gtccccatga atttttaatg cgatatgcat gtttactatc atcccatcca actatgataa
    12001 tgccatggtt aacatcttta tcgctgctta ccggagtaaa ttcatcaaat ataccgccaa
    12061 catatgcctg aaatgccgga gtaaccttaa cggtggctgc tattggccca taggtacata
    12121 atgccttctt catctgctct actgttggaa tacctgcatc cgcacgcaca tacccccatg
    12181 caaccatttt tatgggaaac tgttttttag gtgtacatcc atattcatac cccctgtatg
    12241 gagcaacctt ttcttcaaca gggtaattat tcatcaaaaa atcaaaaaca ttgccatacc
    12301 agccgccatt acaactgccg gcatctctgc catatttatc acgagcacaa tcaatgcaat
    12361 actgctcgga taaatcaatt tcatcttttg cttttatctt gtagcacccc tccacaacac
    12421 ccatcgcggt aaatgaccag caactgccgc aaagcccctg atcctttatt ggtgtcatca
    12481 tccccaatgc aaaccagtcg agcgctggct ttgatggatc ggcaagcata aatgactcgg
    12541 ggagtgtttc ccgcggcttt tcaggttcaa ctgctttggg ctcttctttt tttgcttctt
    12601 caggttttac tggttctttt ttaggtggaa gtgttgcctc tttttgcttc ttagcctttg
    12661 attcagacaa acgttgcaaa aattccagga aaagctgtaa tccactatta gtctgaattt
    12721 cagcttcttt ttcaagatct tcaggtggaa tagctccggt aatttcatct atctgccgct
    12781 ttaatgcttc agtcatatca actttaaatt taagatttcg ctcttttata tcttttttaa
    12841 ttatggctaa ttgctgtttt acctcagcaa tacttttcgc atccctctgc ttctgagcct
    12901 caacactggc tttaaatgaa cttttcattt gtgcatgaga aaaagcaaca ggagctatca
    12961 acatacaaac agtaatactt gctgcaatgt gctttttcat atttattatt cccccctgaa
    13021 attgaatatt gtgttattcc gaggaagcgt tagcgaccga agaatctatt cattatatat
    13081 tcattcataa aggagattct tcgctatcac tcagaatggc tatcggcttt gcctcagaat
    13141 gacaataacg ctacagcttc aacccgcgtt tggtgatcct gttaaaccac caatggctat
    13201 acgcggccat cagcaactgc actataatta cacaatgtta ttatcaaata tactgaacca
    13261 aatatataat gatctatagc aaatgtctat tccttttttt attgaaaaat atttaaattt
    13321 ttgatatacc ataaccgata aaaaagtgta atagcataat gtaagcataa cgtgcatctt
    13381 atcataagag ggtatccact atgaaatcaa tacgattttt atttactgtc accatcactg
    13441 tctttgttct tgcagcggtt tcaggtatcg tcattgcaca acaacaagct gcaccaaaag
    13501 aaccacaaaa agaaggcata aaagaagaag ttaccggctg gcgcatcaaa gattttaaac
    13561 cctatataaa agcgatagaa gagcttgaaa aactcagcaa ggaatattct gataatcttc
    13621 ttaaacttgc aatagatgaa tattcaacgg gtatagatat acttgaagac atggaaaatg
    13681 aggttaataa attagtagaa acaaatcaaa agaataaaaa cctcaatgag cagtggtact
    13741 ggcaggaaat agacaggcgc gaacaggaaa agcgtcagat atcaatgatg aagtttgaag
    13801 caaagagcaa agcaataacc tactttacac gcgctatcaa ccatatggat gaaataacct
    13861 ttgaggaagt aaggaaagac ccaaaatttg ttaatttcca gattggatta tataaggcat
    13921 atgtatccac gcaatatgat ctttacaatt taaaaccatg tatccctatc ctggagcgct
    13981 acattgctat taacgaccaa actaaaaacg atgtatgggc atataagtat cttgcaagct
    14041 gttatggata catggaaagt gttctggtaa aatacaaaca gggtacagag caggagcagt
    14101 tgcaatataa gcaaaagaag aatatatata tgctcaaagc tgccgaatta caatacggta
    14161 ttgagtcgcc ccagtacaag catttacgtg agatagtatc tcaggatgaa aagaaaagtg
    14221 aaaagataaa tgaatttaaa tagcattaat ttttagcaat tcttccacaa taaaaaaagc
    14281 aggctataaa agcctgcttt ttttattgct ttctactttt gtgactctac tttaaaatga
    14341 gaatgaacct gatattctgt accattgaat atcattgtaa atacaataac cctgccggga
    14401 tacacatata ctgtaagagg tttcggctgg cgtatgttat tgtcacgtat gtatttattc
    14461 tcagtttcat caagacgcca gcgttcaata attatttcat ggtactgaca tgcagtcaaa
    14521 tttaaatcaa ttgctgtgct gttaatagat aatccgggct cagtcctggt atagtccacg
    14581 ccatcgcaca ttattttata aaacactctg ccagcctttt cttctgatgg catatcgtag
    14641 agtgctatct caacagtatg gtcttcactg ctgacatact gcacattgct ggcatacctg
    14701 tatacaggct tttgggcaac aacatcaatt acctcggttg catcagtatc ggcaaccagc
    14761 acaatgcggc tatctatcgc ccttttaaaa tatggtcttg acaaaaagta aagcggcagc
    14821 ttaccatcat tatattcaag ataattatcg tttgtaagca ccgccaatcc gccgtccagc
    14881 agcacaaaat cggtaatagc ttctttttgc tgtacaatac cgatgttaac atcggcgcga
    14941 taaagatagc agtttgtaga atcatgaatg ctgatataca ctaccggtgg gtcaaatgct
    15001 atacgcttta cctctgatga ttcgttgatg ctggctacaa cacatacaat agattttttt
    15061 tcatcaagaa gatatgcacc agcggtaact tctttttcac ttcttgccac atacaaaatt
    15121 gctctattgt ttattgccgt aaaatccagc actgttccat taacaggaag cgaagtattg
    15181 ctatcaagat aaataacccc attgtgtaaa gcaaaagctt cacacgcatt accctgcaaa
    15241 caaaagcaaa agtggaaaat aaaaagaagc atccagaaaa tacaattaaa tgtggaacaa
    15301 cctgttttta aaatcatgat atattgttaa cccaagctta ttgtgctcgt caataatttc
    15361 ctgtaatatt gccggcgttg taagcggatt tatggtaata gctcgcagta caacaatctt
    15421 ttgccctcta tagcgtgttg actccagcat ggtgcgggaa acaaagctat tatcttcctg
    15481 gcgtattgct ctatgcagtt caatattcat ttcattgata ccatcgttga gtttctttaa
    15541 acgcttttgt aaccgtgttg ctttttgctg gtcggcattg ctcaattgtt cataaattct
    15601 gttaattttg ctttgaacac cttcaggaac aaatcggtaa ttggtgataa ataattcagg
    15661 gctgttgagc aattcaaaat tttcatgttt attgatgata tctttcaacg tactggtaag
    15721 cataaaggca tgatcaaaca acacctcaaa acctgtggtt ccaaatatct ttaaggtaac
    15781 ccatggcttt aaacatgaaa atgggcgtga tccctctaca gtaaacctcc cctgatcaac
    15841 agaatcggga cgtatgatat aatctgatgt atgcatgata tttagaagat catagggatt
    15901 tttaaacaat atcaatccca tggacaatgg cgaatataac aacttgtgag catcaaatgt
    15961 taccgaatcg gctttttcta tacccctgaa aagatcgcga tacttgttta ccagtaatac
    16021 tgaccctccc cacgcagcat caacatgaaa aaatatgttg tattcatcgg caatcttccg
    16081 taaggccata agatcatcaa tgttgcctgt ttcggtagtt ccagcaatac caatcaacga
    16141 tatgactttt attgtatcat gatggctgct attatattcg gatatctctt ttaatacttt
    16201 ttttagtgca ttaatatcaa ttttattgtt actatcgaca ggaattttta taacattatt
    16261 gtcccctatc cctgtgatgc gggcaacttt atctattgag taatgtccgc gctgcgaaac
    16321 aatgaacaca gctttatgaa tggcatagta gcgaaaagtt tcagccacac cagctttgcg
    16381 tataccggga aaacgctcat ctgccggaaa cgctttatta cgtgcaacca gcatagcggt
    16441 aagattggcc aatgtaccgt caagagtaac atttcccagc gcaattctgt gattttgaat
    16501 atttcgttta taaaatgaag ctgatttgca gaaaataagc cggtgaatcc atgaaataaa
    16561 ttcacgttcc acaaatgttg atgcctttgc tgtttcaatt tttacctggt tttgatttaa
    16621 tgctgcaatg atcatctcaa gtaaaatcat aaaataagga atagcactgg tcatgtgccc
    16681 tatataataa gggttgccaa ccttcaccga atgtttaatg accttttgct ttacctctgc
    16741 cagtatatct ttcaacagtt ttggctcttt agggatatcg atagcattaa acaaacaggc
    16801 taactggggt aatgaaattt cgctgtggat gccccccttt tccttaaaaa aggaatgaat
    16861 caaatcaaga agctcatagc caaattgcat aaatctatcg ggcgagtcgg gcattataaa
    16921 aagcttgcgc agatgctcta aatcggtggg aatcttactt tgaacatgct ctattgaagg
    16981 ctcagccaat ctcatcggga taaccattgc tcataaggtt ggtgatgact atgttactac
    17041 aaaactatag ttaaaaaatt aatcaagtac ttttgtttag aatatacaaa tttattgctg
    17101 atacctataa caatcatcca ccagaacaat acgtaccggg acattatgcc gtataatcga
    17161 cccgctaaaa atatacctcc ttactataaa ataactaatt tttattattg tataatgttc
    17221 gatagttact gtataacact gtacacaagc tgtgaaataa taagggtatc atataaaaaa
    17281 tagtatcctg acggcttttg ttcgatagtt actgtaaatg ctggtatatt ttattgaagt
    17341 gaagtgaagt gaagtgaagt gtaaggagtt tgcgatggct ggcttgttgc tatccggatg
    17401 tcatgaaaat atttttcggc agtcatcatg aaccctgatt caggatatct gaaaattatc
    17461 gggattccgg ggcaagcccg gaatgacatt ggcgcataat tctattttta aggagctatc
    17521 gcaatggcaa ttcttaaact tgaaaatgta tctttgagct taaacaataa accaatatta
    17581 gacagcattt cggtagattt ctgggaagga tatatccatg ccattgttgg tcctaatggc
    17641 gccggtaaat caacgctatc ctatgtcata atggggctta acgattatcg caccatcagc
    17701 ggcacattgt attttaaaaa taaaccaatc aatgaactat cggtatacca gcgggcacgc
    17761 cttggcataa gccttgcatg gcaggaaccg gcacgctttg aaggattaac cgtttatcag
    17821 tatttacagg cttcaaaaaa ggataatgga ttatcaatag aagaagcaat ggatgttatt
    17881 ggtttgccag ctcaggaata tctgccgcga gcatgtgaca aaacactaag cggtggtgag
    17941 cgcaaacgca tagagctggc gtcactgctt gtgatgcagc cggaggttgt tttgctggac
    18001 gagcctgact caggaattga tgttgaagca ctggagcgga tattcgatgc aatggcaatt
    18061 ttaaagcaac gaggctctac cataataact atcacccata gtatggccgt tttgcaaaaa
    18121 gcagaacatg catttttgat gtgccatggc aatatcatcg ataaaggtgg cattgataaa
    18181 atttcaggat attttgaaaa caaatgcatc ccctgcgatc ataaaaatat acctatttct
    18241 ggagaagcac gctaatggat gatagacgag cagttgcaca aaccttatac aaaaccatgg
    18301 gattaaccga cgctgcactc aatgaccctg atattgcgca tctggttatc aatagcaata
    18361 tggttgttgc ttcacacctt gttgaagggc ttgttgttga gccggctgaa acacaaaacg
    18421 gcgtgagggt aacaatgagg gtgctggcgg ggcatataat acaaaagccg gtacaccttt
    18481 gttttggcat gctgcctgaa gatggactgc aggaaattat tatggacgtt accattgaag
    18541 agcatgccag tgtttccata cttgcccact gtacattccc caatgcaaaa aatataaccc
    18601 acaagatgga ggctgttatc accattaagg aaggtgccag ctactcgtat cttgaaaggc
    18661 atgtccatgc agccagcggt tttattaccg tgcttccgca tgcgatagtt actgttgaaa
    18721 aaaatgctac atttaaaacc gactttgaat tgttgagggg taaagccggc attattgata
    18781 ttgattacga aaccacctgc tatgagcata gccatttaga gatgaatgca aaggtaaacg
    18841 gcatacagga tgatgcaatc accatccgcg aaacaggaca tcttaacggt gaaggtgctg
    18901 tcggtgtact taccacccgc attgccgttc gtgatactgc acaggctact gtgtacaata
    18961 ctttaactgc acacgcagcg tacgcgcgcg ggcatgttga ctgtaaagag attatacagg
    19021 atagggcaac tgctaaagct attccggttg ttgaggtata tcatcctaag gcacatgtta
    19081 cccacgaggc tgccattggc agtgttgatg ataaacagtt gcaaacgctc atggcccgtg
    19141 gactttctga ggatgaggcg gttgatttaa tcatacaggg tttgttatca gggagttaaa
    19201 tccttatctt tccttcatcc tattttttga agtcttctca atgacaagag ctatcgaccc
    19261 gagtagtgaa agcaaaacaa aaaacaccat tgtttttatt gataaatcac tgccctgttt
    19321 tctgcgctca agtaaaagct caagatacat tgtatcgttg acatcaaaat cttttttttc
    19381 agcaactcgc aaatcaaaat cttttaatat atactcaatg cctgcaccaa cctcatcata
    19441 acggttatag ccagcaaaga tgtggggttt gcgaagcatg atgtatgcct gttttaattg
    19501 ctgtgacgac ggattatcat ggtagataag cgattcaatt gcgggctcag taataaaagt
    19561 tgaatacagc ccgggcaatc caaacaacag tgcaacaata aacatcgcaa tggtaaaata
    19621 agcataaatc tttgtatcat caaatgtcat ttttccacct ctttaagata cgtaacaata
    19681 tctacagttt tataggtaac attatcaacc cgtaccatcc ttgcaggtaa cacaagagca
    19741 gcttcacgct gttttattaa ttcatcgacg ctaatctcca gagcatctaa taataccttg
    19801 atagtatcct cgctatcttt ttgttgaaca aacaactgtt tttgctgctt tggcaaaatt
    19861 tgctctgctg ttacggtaca ttgttttact gctttttgtt cttcatcaag tattgataca
    19921 acagcactat agctggtaat caccgtatca acacttttat tattgatata tattgtgtat
    19981 gttgatgcaa acactggaac attcttttca aatgtaacat taacagtaaa ttccacatcc
    20041 cccagctcaa catagcgcgt gcagcttaaa cacaccacac ccacgcaaat gattaatatc
    20101 ttcttcatta catccctcat taatgttatt gtgcagttat gaaataatta tgaatattta
    20161 gtcaagtaaa atgtatggat agttacagtt attccatagt tattttgcag caatagtgca
    20221 gtgttaataa aaatgcaagc tcaagattta tctttaacaa ctgtatactg caaaaattct
    20281 tattgagact gtattcttat ttacaataaa aacatacata ttaactggaa atctaaattt
    20341 tagcaacgaa ggtacgtttt ataagggttt taaatatact ttaatcttct agcattaaat
    20401 gcatttaacg taccttctaa tgtgtcagaa gcatgtttat taattgcaga attttcaatg
    20461 gatccgatct caaatgtaaa tttattattg tgatctttat ctgttttttc atttaattgt
    20521 ttttttatta tctcaccact tttccatatt aattcagctt taaaatttga tgccagatca
    20581 tctctcaaag acctatctgc ttttagaaag aaaatcacgg taattatgct aattcccgca
    20641 taaacttatt atgtctgtta atgcactttt accatttcct ttatttccaa taatagctac
    20701 cagcccataa ttcaagggaa tttcaatatc tttaaaccaa attccttgtt cttcattata
    20761 tccgttcatt tgatttattt tcaactgctt aataaatttt gttttgtttt ctttaacttt
    20821 ttttaatgca tcaggttctt ctcttacata aactcttgcc tctggttcaa agattatttg
    20881 ccttaatcct ttaaatgttg ggtctgcttt aatccatgtg taacgtgaaa ataaattttc
    20941 tatagcatga gcatcagaac caataataca aggttttaag gatttaaatt cttttgtgaa
    21001 ttttttttct ttttcttcta ttgtttcacc ttcgctttta tgccctaatg cccaatttat
    21061 ggtattttca tttgtagaaa ataagatatc agctttttaa ataaaaattt tccttgttaa
    21121 atgattctgc ccattccaat ttaaattaga taaatcttca tcagcaggta caagtgtgag
    21181 atatttattt tcaaataaag actttttatt agaaaggata cttaaaatct ctgtatcatc
    21241 tacaacagca ttcatacaac caatgtataa atctgagtca tctttaaaat tcttatgctc
    21301 gctttttaat ttttcatcga gttgtattag attatgcttt gttaattttc ttttctcatc
    21361 ttcattttga ggattacttt cataaacaaa atttatttct tgtaaaaatt ttctttaatt
    21421 tcttttattt caatttcatc agagaaaata acatgaaagt ttatcctatt cttaccgacc
    21481 aatttattta atcgaaattc tatatttgga agaatcaaaa ttctttttac ttgttcaatt
    21541 ttttcatcaa ataatttttt taatttgtta tcgttgctaa tataatcatt ttttattttt
    21601 catatccgtt tattatgaaa taccaatagc ttcaattgta ttttcaattg ccttatcgaa
    21661 tacgttttta acataattat cccacgtttc ttcagattct ggatcaacaa attgattatt
    21721 taatactgaa tatgctgtat aaacgtgtaa atctcatctt ctccaaattg aactctatgt
    21781 attcatagtt taatctttat ttttaattta aacattttta agcactacct tacctcagca
    21841 tctctcaaat gcttaatcgc aatttttata tatctgtcat cagagaatct ggttcttttt
    21901 ctttcgctcc atttgttgag ttcttgctta atgacatcta tatcattggt attttttttg
    21961 ttcatta
//
LOCUS       DAOSOX010000019        21943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2371_length_21943_cov_8.605537, whole genome shotgun sequence.
ACCESSION   DAOSOX010000019 DAOSOX010000000
VERSION     DAOSOX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21943
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_21943_cov_8.605537"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLX22_02640"
     CDS             complement(<1..188)
                     /locus_tag="PLX22_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18833.1"
                     /translation="MDKQNFPVALSLMSSGLWQLYAQNDPGTIYYECVLPEQRTVQDY
                     YATWYSGTPLEESEKIIE"
     gene            complement(195..938)
                     /locus_tag="PLX22_02645"
     CDS             complement(195..938)
                     /locus_tag="PLX22_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18834.1"
                     /translation="MNSKRGDVMTGFRKITVTLLIVSILIAIVIIFDGEIVKKIVMLK
                     PVRVVTQGEINRMEEELSKLTKKDAKTPETINKIGVLYQNLGAKYNEKGAWDPAIDAL
                     QKALGHGRNVPVVHYQLAIAYANRGTQLGNNDDIDKAVYHYEKALQMEPNYIEAKYGL
                     AILLFYRKNERSKAIQIMNEIAKQNPKFYRARFALARFYYENKELDKSLSVYQDLYTD
                     LDKMDSTLATEYRQACKENIQRITSEMAR"
     gene            1170..1367
                     /locus_tag="PLX22_02650"
     CDS             1170..1367
                     /locus_tag="PLX22_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18835.1"
                     /translation="MLPHQMRAGDFACLLCGSKLNLSISDIAVGITTGSCPMCSQPFT
                     IKLNKKDIELLLEAEETGKQQ"
     gene            complement(1371..1883)
                     /locus_tag="PLX22_02655"
     CDS             complement(1371..1883)
                     /locus_tag="PLX22_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT18836.1"
                     /translation="MIQDYFEKFKRWYLFNTEKIKYVGKKNDKHTCILCAIRDKAEDV
                     VNLEVHRTERFIVSINLYPFNPGHCMIFPLRHCENIHELGKEDIVALHELTIKVIDII
                     KQEFNPSGFNVGFNIGLWSGASISHIHLHIVPRFPNELGFLDVISHTRVMVVDPIVVC
                     ANLKKRFNAE"
     gene            complement(1902..2771)
                     /locus_tag="PLX22_02660"
     CDS             complement(1902..2771)
                     /locus_tag="PLX22_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOT18837.1"
                     /translation="MKRHYILLLLFLFFICSIFMVYAQKNVGDSSKDVNYCIQCHLTL
                     NGIPRSVVLEWENSIHAKKNLECDGCHGGNHTVNDVKQAKDKKTGFIGKPVKKDIPQF
                     CGRAECHGYALTQFKRGPHYDSVLKIGSPNCVDCHGDHNVQRSTYHIISDKTCSSCHS
                     VEYSRELVSSMINIEKNIEDIENSLEYLNQRNVESKDSMVRYLELKRYFHQLVHVFSK
                     QEIEFTKKLVEVEIEYLDKQLTNRIATIKRLDLIYIITSGFSIAIIIAFTVYIALIRK
                     RRTKVMKNFQSRL"
     gene            complement(2841..>3357)
                     /locus_tag="PLX22_02665"
     CDS             complement(2841..>3357)
                     /locus_tag="PLX22_02665"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOT18838.1"
                     /translation="LCHPMQRRIKLLMFWKEFLFTGLYTGYFPKGSGTVGAFLALLFY
                     IIIHQLCGKYGIMVNLILVTVILYPSIKLGDDAEIFFAMKDPQAVVLDEMLGFWVTML
                     FHSFSLLSVLLGFIFFRLFDIMKPYPIQKLEHCKGGLGIMMDDIIAGVYANVVISLIL
                     FIMNIYGIVLV"
     assembly_gap    3358..3457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3458..3660)
                     /locus_tag="PLX22_02670"
     CDS             complement(<3458..3660)
                     /locus_tag="PLX22_02670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT18839.1"
                     /translation="MNIPNLLTLSRIILIPLFLYLIFTPTIEHRIWALVIFVIASLTD
                     LLDGWTARKLKQETETGKFLDPLA"
     gene            complement(3657..4952)
                     /gene="rimO"
                     /locus_tag="PLX22_02675"
     CDS             complement(3657..4952)
                     /gene="rimO"
                     /locus_tag="PLX22_02675"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOT18840.1"
                     /translation="MPQELSYYIISLGCPKNEVDAERLNADLHNAGFVPAHGMDDANI
                     IFINTCGFIKEAKEESIATIMDAIDYTAHAATKKLIVFGCLTQRYKDEIIHEIPEIDL
                     VWGLYDSSMINEVARICTVDLLTVTIAQKRKPLVDNIPYAYIKIAEGCSNNCSYCAIP
                     IIRGKHRSYAPELIVDDAAQALQRGVKELIVVAQDVAIYNYNGVLVNDLLHKLNNLDG
                     TFWIRLMYCHPDHISSELIEAMATLQKVVHYIDIPFQHASTKILRAMNRKGNAKRYLR
                     LVEKLRNAIPDIAIRSTFMVGFPGEDEDDFTVLLDFLQEAQLDRVGVFMYSPEEGTKA
                     SGMSGMVKKATQHKRYNRLMQLQQTISLQRLEKTIGNVVEVLVEEKVDANNYIGRTQY
                     DAPDIDGIFFLTADGINVNDIVLAKITDTLEYDRIGVLI"
     gene            complement(4960..6096)
                     /locus_tag="PLX22_02680"
     CDS             complement(4960..6096)
                     /locus_tag="PLX22_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT18841.1"
                     /translation="MVAEIGETLRNAREAKRLTLKDVSKDTNIVVKYLEALENEEFDK
                     FPSETYLLGFLRSYAEYLRLDADELIQAYKGYKIGESSTPLEELTRPTGLSIFTQLRM
                     YAEQYKNILYIAGIGVAVLIVIALFISLITSDVHIGSDDSIKAIQEEYYRTQGKKIAI
                     KNIWPLQIVNDSGIVLLYNNEACQFLVENKEILFLLNEIKDDSVVVEFLPDSKKYILE
                     MEKSVVCSPMESSQNIILTLKGLTENRAKIKVQLTAAQQAVEQEEAVSVVTPVTPGTE
                     VVTHDQKNLKIVLETEFIQKTYFELYLDGNEKVRGFIPAGNKERWEASEYIQIKIGNA
                     GGVKVKINNVDYVFGLPGQVANKVITWRKDPTNPNKYKLEVKDW"
     gene            complement(6100..6795)
                     /locus_tag="PLX22_02685"
     CDS             complement(6100..6795)
                     /locus_tag="PLX22_02685"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOT18842.1"
                     /translation="MSIAKKKNFIILAIVLLIFVPCIMGYPYKFDFITVNDIVKQIKK
                     AFSDVDSYQASFVMISDRIGKRQTQSGTIWYKSPHYLRVDFDSPYGQRIVSDGNQVWI
                     YIPSLNIVAEQDLNSGTLFSVGTGPGLNRLFSKYHYRFDGKEQPREESNNRYYVLFLK
                     QKETRSGFRTLKLWVNEQYFITKAYGETASGKNITLEIKNVTTKIDVKKGFFKFDPPS
                     NARIIKNPMLVEE"
     gene            7005..7175
                     /locus_tag="PLX22_02690"
     CDS             7005..7175
                     /locus_tag="PLX22_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18843.1"
                     /translation="MGSIKQPEIQQEQQHPETIVSQWNNFKLLESQQIYFSVGKATIK
                     HILIKREPLIGA"
     gene            7184..7708
                     /locus_tag="PLX22_02695"
     CDS             7184..7708
                     /locus_tag="PLX22_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18844.1"
                     /translation="MVNKRFVIVFTVVLLSVQPLHSHVATSSIIEENRDFWNQLIQTI
                     TIANKNLLYASYVAYKDKLYDLSIDSFQECIAQNPDNQLIQGVANYYIGKNFYHIGEY
                     KKAALFFLATERYSMNQYSSLKAAALINAALCYIAIDDIALAKTYLQKSLQYDIEGTY
                     RTTADSLLQSIKEH"
     gene            complement(7705..8712)
                     /locus_tag="PLX22_02700"
     CDS             complement(7705..8712)
                     /locus_tag="PLX22_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOT18845.1"
                     /translation="MNILVTGSAGFIGYYTALKFLQQGYTVIGVDAINSYYDVSLKEA
                     RNALLLNDKNYIFYQCDIANYEELSHIFKKHTPDYVVHLAAQAGVRYSLDHPFEYIQS
                     NIVGFMNIVECCRHNPVNHLVYASSSSVYGLNSCVPFSVHDTVDHPVSLYAATKKSDE
                     LIAHCYSHLYGIPTSGLRFFTVYGPWGRPDMAYFTFTKSIVEDKPINVYNYGDMERDF
                     TYIDDIVEGVYRITFIVPQGKPHFDTMHPDPASSTAPYRIYNIGNNKPVSLLYFIEVL
                     EKEIGKKATIHFLPMQPGDVLATFADIDELTQATGYSPVTSIEQGLSLFVQWYKKFYT
                     V"
     gene            complement(8745..8963)
                     /locus_tag="PLX22_02705"
     CDS             complement(8745..8963)
                     /locus_tag="PLX22_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18846.1"
                     /translation="MIHDSIHEYNENVYTTFLKGDAREESYYQYLAKLVYDISVSIGY
                     TAVLITTLPKKLMQEIQTLECGMGKAII"
     gene            complement(9195..9614)
                     /locus_tag="PLX22_02710"
     CDS             complement(9195..9614)
                     /locus_tag="PLX22_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HOT18847.1"
                     /translation="MSKKFTIHFRKLTHYKYQLMENYIHATSITLQQECCIDDFVALK
                     KDGQLYIFKGYAWDGPSGPTIDTKDFMRGSLVHDALYQLMRKKQLPVSYKDYADKLLH
                     DICIEDGMSKFRAWYVYQAVRLFGKGALKPDDDINNT"
     gene            complement(9834..10760)
                     /locus_tag="PLX22_02715"
     CDS             complement(9834..10760)
                     /locus_tag="PLX22_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOT18848.1"
                     /translation="MNDKILLIVNPIAGRGRAAKSVPQIERDFNRLGIKYDIVFTIRQ
                     WHAAELAEDAAKKGYKAVVVASGDGTANEVLNGLMLARKKGNNKTAMGQIPVGTGNDF
                     AYGMGLSDDIDECIKVIADYTLRKIDVGFIKGGDYPQGRYFGNGIGIGFDATTGFVAS
                     RIRFLSGMLVYFIAALQTIFIYYNAPRIRLSYNDKETIMEALQISIMNGKRMGGGFHF
                     APNGSPFDGLLDVCMVRSMNQFKIFSMIPYFIKGTQGKRKEVLEDRTTKIAVVAEKGN
                     LPVHCDGETICYAGKELFIQIYPAVLNFITKA"
     gene            complement(10849..12279)
                     /locus_tag="PLX22_02720"
     CDS             complement(10849..12279)
                     /locus_tag="PLX22_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT18849.1"
                     /translation="MDSIQAPVLVVDASKHIIDINNHARRLFHTMDIVGKSCKELFPD
                     CEKDGNCPVDDADYKIPTDCEKRKLSMIVHTVKEQMDIIIAPAKKEDDLVFLHIVTDS
                     DIQNRQNLVMLEKNMTISTLASGIAHEFNNMNAGIQGLIELLLSQEELSNAGIKDLRT
                     VLRIVKRATHLVDQLSMLAHKKPSKQMLSNIEDIVNDCVSIIKPQFIDAGISIEVINK
                     NKLSELFLDANKITLALMNLLLNARDAMIDSAKKQLSITTYQDKENIYIEIRDTGCGI
                     KAEYLQRIFEPFFTTKGPLGHSNIPGTGLGLSVALGIVEEHNGAIHVESTPGEGTAFK
                     LLLPLNTTEKVDSEIAVNYNSYNFLKKKILIIEDDVEFATIIQRALHAKNAEVFITNS
                     GAEGLNFLDAHGADLVLLDVHLPDMSVWDILKKIQYIANRPNIIIVSGNYLAMNNNYV
                     GIVDYVLLKPFDLDELFTAIKNSLSL"
     gene            12570..13490
                     /locus_tag="PLX22_02725"
     CDS             12570..13490
                     /locus_tag="PLX22_02725"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOT18850.1"
                     /translation="MNTFDNTITVGIVGAGAMGSLFAFLLHPYATVVMYETNKTTVND
                     IKKNGLSIEYNTRTHAINPMISNNPGILAHADCIFIFVKSFATKSAIEAIKPYIKSDA
                     IIITLQNGIGNVPVIQSMVNNPLVYGTTTIGATRLTAAKVRFGGEGQIIIGGDSYRHN
                     SFLYSLLQKAGLQVTITQNPDKALWEKAIVNAAINPLGAILEVPNGALLYHDYIKDIM
                     RNIIMEAADVGLAQGIAIHGNDMITTTFQVCTNTKNNYCSMLQDISAKRITEIDYING
                     AIIKTAMHYGIATPYNATVYRLVKYKELLL"
     gene            complement(13540..14628)
                     /gene="ispG"
                     /locus_tag="PLX22_02730"
                     /gene_synonym="gcpE"
     CDS             complement(13540..14628)
                     /gene="ispG"
                     /locus_tag="PLX22_02730"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT18851.1"
                     /translation="MSTTPKKTITVHAGNLAIGGDAPVSVQSMTNIPVSNVAKTIEQI
                     IKLEQEGAQLVRLAMRTEEDAKYISQIKHEVNVPLCADIHFNYKIAIKAIECGIDKLR
                     INPGNIGAKENVIELVKAAREKNIPIRIGVNSGSVDLKKYGQVNPDTLVASAMEHVHI
                     LEDCNYTTIVVSIKSSDLLQTIEANRIFASRRQYPIHIGLTEAGYGTDCTIASSMVIG
                     TLLYEGIGNTIRISMTGDPIHEVRIAKRLLEIGGFRFSPFTIIACPTCGRTDEQLDIL
                     GLAQEVTQMMHDRFEEILKKKLHHITVAIMGCEVNGPGEARHADVGIAGARNGRVVLF
                     AKGEKITMLPAKEAIKAIAEQIAILSGC"
     gene            complement(14647..16209)
                     /gene="rseP"
                     /locus_tag="PLX22_02735"
     CDS             complement(14647..16209)
                     /gene="rseP"
                     /locus_tag="PLX22_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT18852.1"
                     /translation="MIAITYILAAVVLLGLCIFVHELGHLLGGKLVGIKAEVFSIGYG
                     KGFIKKKWGDTTYQITLIPFGGYCKFYGEDPTEAREGKSYEFLSAHPLKRVVTVVMGP
                     LFNLFFGIILFFVMNVYGYPVETNKIYIPEYLTKGEYVSPAYTAGLRTGDIIVAINNE
                     QVISFIDIQSKIAFSKGRPIVIKALRGNDSFEVTITPKKYTKKGYYTIGVMPYGNRVL
                     VMGILENSPAQKAGLKELDEIIEANGTQFHSPEEFTSFVRKNPLKKLSLTINRGGTIM
                     TVPVTPIQKDVLTITDFTDLRFPKEKHSITVDSLSLIKAAADKGRVKVNGYTVTSFTQ
                     MKKIIASTNGVVTLQTPGGNYRGIMKFESFGFVGIETAISPENKFIQYTITDAFVKSM
                     VDPFDFIALNFRGIGMMFSGELDVRQNLSGPIRIAKIAGDTAYYRGVSAFVVLMAKIS
                     IILMVMNLLPIPMVDGSYILIFLYEAVARRAFNQKLLEKIQMIGFAILVLLSIFVIFN
                     DLSFLPFFKDLF"
     gene            complement(16218..17372)
                     /gene="dxr"
                     /locus_tag="PLX22_02740"
     CDS             complement(16218..17372)
                     /gene="dxr"
                     /locus_tag="PLX22_02740"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT18853.1"
                     /translation="MSKQIIILGSTGSIGISTLKVIREFNNQFVVKGLSCHTNITLLK
                     EQIKEFHPDFVAVSDEQVCLTQDYKQLKKHFCSIVFYEGMDGLNQLVSNDADILVVGI
                     VGGAALKPSINALPHIKRIAIANKETLVMAGDIFLHFLQHYNVELLPVDSEHCAIFTL
                     IDGKPVNTIQRIILTASGGSLRHKAVETLGFVTVEEALMHPTWDMGKKITIDSATMMN
                     KGLEVIEAHYLFNVPYNAIEVIIHPESIVHGMVELNDGTLLAHMSITDMAYPIQRALT
                     YPDICHNPFGNVDLTLMGSLHFSKPDMARYPALALCYEAGKMGGTMPAVLNAANEVAV
                     QAFLQKKITFTKIVKVVEKVLQKHNCIENPDLGMIFDADEQARQFALQCI"
     gene            complement(17391..18278)
                     /locus_tag="PLX22_02745"
     CDS             complement(17391..18278)
                     /locus_tag="PLX22_02745"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT18854.1"
                     /translation="MRERTKNTMIRILTAVVALPVYVFLIITNMLQSLPILIVSVIVS
                     GVCLWEFYQIAEGTTGYKPFVKTGIIFGVIINMVMYSYAYGNITGIGGITLNFDVRFL
                     LAVVTIAVSVLATIHIFKRPLKGGIYSIGATILGWFIIVLPYAHIMLLKSIRDGVIFI
                     IFIHLIVMINDSGAYFGGVYFGKHKAQFKASPNKSWEGYFSGLLFGIVFAILYSQFIE
                     IFYGRYLFSIIEAAIIGIIFSIIGNIGDLLESALKRDGSIKDSGSIIPGHGGMWDVFD
                     STIFSMPLFYYYLILRGII"
     gene            complement(18275..18997)
                     /locus_tag="PLX22_02750"
     CDS             complement(18275..18997)
                     /locus_tag="PLX22_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT18855.1"
                     /translation="MFANYKKKIDYAKIPKHIAIIMDGNGRWAKKRGLPRIEGHRRGA
                     EVIERCMDSAIELGIGVVSLYAFSTENWARPKEEINGLWKLLESFFEKKLSTIIEKGV
                     RIQHSGSLSELPPTIQRIIANAVDKSKNNTTIVLNFCLNYGGRHEIIHAVNNWLQQRP
                     SHELSLTPEIFENYLYTAGLPNVDLLIRTSGEYRISNFLLWQIAYAELIFMKVLWPDF
                     NEQHLYQAIYEYQHRERRFGGL"
     gene            complement(19028..19585)
                     /gene="frr"
                     /locus_tag="PLX22_02755"
     CDS             complement(19028..19585)
                     /gene="frr"
                     /locus_tag="PLX22_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT18856.1"
                     /translation="MIKDILDDVKNRMQKSVQALAKDFTTIRTGRANPAIFDNVKVET
                     YGTEMPLNQVATISCPEPRLVVIQPWDRTNLGAIEKAILKSNLSLTPNNDGNIIRIQI
                     PELTEERRKEYVKMIKQKAEDCKVAIRNIRRDGNDMIKDLEKSKDISEDEAKNALDEV
                     QKLTDKFVGEVQMMTDNKEKEIMSL"
     assembly_gap    19598..19697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19698..20375)
                     /gene="pyrH"
                     /locus_tag="PLX22_02760"
     CDS             complement(<19698..20375)
                     /gene="pyrH"
                     /locus_tag="PLX22_02760"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT18857.1"
                     /translation="MEEESQLKYKRILLKLSGEALAGQNKVGINPEIVSHIAREVKKA
                     YAMGVDIALVIGGGNIFRGSVGTSIGIERATGDYMGMLGTIINALAVQSVLENMQIPT
                     RVMTAIEMRQVAEPYIRRRALRHLEKRRIVIFAAGTGNPFFTTDTAAALRAIEIGADL
                     LCKATKVDGVYDKDPVKFPEANKMNTVSYMDVIKQQLGVMDYTAVSLCMENKLPLVVF
                     NLFDEGSL"
     gene            complement(20417..21016)
                     /gene="tsf"
                     /locus_tag="PLX22_02765"
     CDS             complement(20417..21016)
                     /gene="tsf"
                     /locus_tag="PLX22_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT18858.1"
                     /translation="MADITSDMIKELREKTQAGMLDCKKALIECGGDMEAAVDYLRKK
                     GLAAANKREGRIAKEGIIASYIHNNSKIGVLLELNCETDFVARNQDFQNLAKDLCMQV
                     AAANPLYVSPEDVAADVLEREKEIYREQLKESGKPANVIEKIVEGKLQKFYSEVCLLE
                     QEFIKDNKVIIKDLIKNKIATYGENITVGRFVRFQIGQR"
     gene            complement(21064..21879)
                     /gene="rpsB"
                     /locus_tag="PLX22_02770"
     CDS             complement(21064..21879)
                     /gene="rpsB"
                     /locus_tag="PLX22_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT18859.1"
                     /translation="MSVISMKALLESGVHFGHQTRRWNPRMAQFIFTARNGIHIIDLQ
                     KTMQRLKLAYAAMKEIAAKGGRILFVGTKKQAQAAVKEYAQKCGQFYVSERWLGGLLT
                     NFKTVSRSIQRLKELEQMEITGKWDAETKKEILELTRELERKRKVLEGIKDMDKLPEA
                     LFIIDPKREAIAVQEARKLGIPIFSVVDTNCNPDEIDYPIPGNDDAIRAIALFLEVMS
                     NAIIEGQSAGQVTGEAATATIDFVEEEQSEAKPEKDYAEEYADEIDVYEDDRR"
BASE COUNT         6781 a         4526 c         3385 g         7051 t
ORIGIN      
        1 aattcaataa tcttttctga ttcttccagt ggcgtgccac tataccacgt agcataataa
       61 tcctgtacag ttctctgttc cggcaaaaca cattcataat atattgtccc ggggtcattc
      121 tgagcatata attgccataa cccggaactc atgagcgata gcgccacagg aaagttttgt
      181 ttatccatac aacattaccg tgccatttca gatgtaatcc gttgaatatt ttctttacat
      241 gcctgtctgt attctgttgc taatgtagaa tccattttat caaggtctgt atataaatct
      301 tgatagacag ataatgattt atcaagttct ttgttttcat aataaaacct ggctaaagca
      361 aaccgtgctc tgtaaaattt tggattttgt ttagcaatct cattcataat ctgtatggct
      421 ttagaacgtt catttttacg ataaaataac agtatagcca atccatattt tgcttcaata
      481 taatttggtt ccatctgcaa tgctttttca tagtgataca cagctttatc tatatcatca
      541 ttatttccca gctgcgttcc cctgttagca tatgctatag ctaactgata atgcactacc
      601 gggacatttc tgccatgccc caatgctttt tgcaatgcat cgattgcagg gtcccatgca
      661 cctttttcat tatattttgc ccctaaattt tgatataaaa caccaatttt gttaattgtt
      721 tcaggagttt ttgcatcttt tttagtaagc tttgataatt cttcttccat gcgattaatt
      781 tcgccctgag ttactaccct tactggtttt aacataacaa ttttctttac aatttctccg
      841 tcaaaaatta tcactatagc aatcaaaatg ctgacaataa gcaatgttac tgtaattttt
      901 ctaaaccctg tcatgacatc acctcgttta ctattcatac tgttatatct tacatttata
      961 aaactcaagg gacattatta aaacttcact atttaatatc atggtgagca tacgatagtt
     1021 gtaaaacact ttttttcaat tgttgattca attgttgatt caatagcttg ttaaacaatc
     1081 tcaccagagt ataaaaaact tgacattttt atagcaattt atatttacca cttaacctta
     1141 acaaactact ttaccaggga gttgtattta tgctgcctca tcagatgcgt gctggcgact
     1201 ttgcatgttt gttgtgcgga tcaaaactta acctttccat aagcgatatt gctgttggca
     1261 taactactgg ttcctgccca atgtgtagcc aaccttttac aataaaatta aataaaaaag
     1321 atattgaact gctccttgaa gcagaagaaa ctggcaaaca acagtaacta ttactctgca
     1381 ttgaatcgtt ttttcaaatt tgcacaaaca actataggat caacaaccat tactcgtgta
     1441 tgactgataa catctaagaa tcccagctca ttaggaaacc gcggtacaat atgtaaatga
     1501 atatgcgata tgcttgcgcc actccacaaa cctatattaa atccaacatt aaaacctgat
     1561 ggattaaact cttgttttat tatatcgata accttaattg tcaattcatg gagagccaca
     1621 atatcttctt ttcccaattc atgtatattc tcacaatgcc gcaagggaaa aatcatacaa
     1681 tgaccgggat tgaatggata taaatttatc gaaacaataa acctttctgt acgatgcact
     1741 tccagattaa caacatcctc cgctttatcc cgtattgcac agaggataca ggtatgctta
     1801 tcattttttt ttccaacata ttttattttt tcagtgttaa aaagatacca tcgtttaaat
     1861 ttttcaaaat aatcctgtat cacacaaata ctccacggat atcataagcg ggattgaaaa
     1921 tttttcatta cttttgttcg cctttttcgt attaatgcaa tatatactgt aaaagcaata
     1981 attatagcaa tactaaatcc acttgttatg atatagatta aatcaagccg tttaatggtt
     2041 gcaatacggt ttgttaattg tttgtctaaa tattcaattt caacctctac caattttttg
     2101 gtaaattcta tttcctgttt tgagaataca tgcaccaatt gatggaaata ccgctttaat
     2161 tcaagatatc gtaccatgct atctttgctt tcaacattgc gctggttaag atattccagt
     2221 gaattttcaa tatcttcaat atttttctca atattaatca ttgaactaac cagctcacgt
     2281 gaatattcta cactatggca actggaacat gtcttatcgg atataatatg atatgttgaa
     2341 cgttgaacat tatgatcgcc atggcaatct acacaattgg gtgaaccaat ttttaatacc
     2401 gaatcataat gaggaccgcg tttaaattgt gttaaagcat aaccatggca ttctgctcta
     2461 ccacaaaact gcggtatatc cttctttacc ggtttcccaa taaaccctgt tttcttatct
     2521 ttagcctgtt ttacatcatt tactgtgtgg ttaccgccat gacatccatc gcactctaaa
     2581 tttttttttg catggatgct gttttcccat tcaagcacta ctgaccgcgg tattccatta
     2641 agcgtaagat gacattgaat gcaataattc acatctttag aactatcgcc cacatttttt
     2701 tgagcatata ccatgaatat gctgcaaatg aaaaataaaa acaataataa cagtatataa
     2761 tgtcgtttca taaaacaacc tcaaaccatt ctggcattaa gcctccgcat actattatct
     2821 atttcgacag cattaataca ttacacaagc acaatcccat agatgttcat aataaacaat
     2881 atcaacgaga taacaacatt tgcatataca cctgcaataa tatcatccat cataataccc
     2941 aatccgcctt tacaatgttc aagcttttga atagggtatg gtttcataat atcaaatagt
     3001 ctgaaaaata taaaacccag caacactgac aacaagctaa atgaatggaa taacatggtt
     3061 acccagaagc caagcatctc atccagtaca acagcctgag gatctttcat ggcaaaaaaa
     3121 atttcagcat catcacccaa ttttattgat ggataaagaa taacagtaac aaggataaga
     3181 tttaccatta ttccatactt tccacacaac tgatgaataa tgatatagaa cagtagagca
     3241 aggaaagcac caaccgtgcc ggagcctttt ggaaaatatc cagtatataa tcccgtaaaa
     3301 agaaattctt tccagaacat gagcaattta atccttcttt gcatagggtg gcacaaannn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct aacggatcca aaaattttcc
     3481 tgtttccgtt tcctgcttca gtttacgggc agtccatcca tctaacaaat cagttaacga
     3541 agctatgaca aatataacaa gcgcccatat tctatgttct atggtgggcg taaaaatcaa
     3601 atataaaaat agcggaatca gaataattcg tgataatgtc aataaatttg gtatattcaa
     3661 atgagtactc caatacggtc atattctaat gtatcagtta tttttgccag cacgatatca
     3721 ttgacattaa taccatctgc ggtcaagaaa aaaataccat caatatcggg ggcatcatat
     3781 tgtgttctgc cgatgtagtt atttgcatcc actttttctt caacaagcac ctcaactaca
     3841 tttcctatag ttttttccaa tcgttgcaac gatattgttt gctgcaattg catcaatctg
     3901 ttgtagcgtt tatgttgtgt tgctttttta accatgccac tcatgccact ggcttttgtg
     3961 ccttcttcag gtgaatacat aaatacacca accctgtcaa gctgtgcttc ttgtaaaaaa
     4021 tcaagcagga cagtaaaatc atcttcatcc tcgccgggga atccaaccat aaacgttgag
     4081 cgtattgcta tatcagggat tgcgttgcgc aacttttcta caaggcgcaa atatctttta
     4141 gcattccctt ttctgttcat ggcacgcaat atctttgtgc tggcatgctg aaaggggata
     4201 tcaatataat gaacaacctt ttgcagtgtt gccattgctt caatcaattc actggaaatg
     4261 tgatcgggat gacaatacat gagtcgtatc cagaatgtgc catccagatt gttgagttta
     4321 tgcagcaaat cattgacgag cacaccattg taattatata ttgctacatc ctgcgctaca
     4381 acaataagct cttttacgcc ccgctgcaac gcttgtgcag catcgtctac aattaattca
     4441 ggcgcatacg aacgatgctt cccgcgtatt attggaatgg cacagtaact gcaattattg
     4501 gagcaacctt ctgcaatttt aatataggca tagggaatat tgtcaacaag tggttttcgt
     4561 ttttgggcga tagttactgt taataggtca acagtacata ttcgggcaac ctcattaatc
     4621 atggagctat cgtataaacc ccataccaga tctatttccg ggatttcatg tattatttca
     4681 tctttataac gttgcgtaag acatccaaat acaatcagct tttttgtggc agcatgagca
     4741 gtgtaatcaa tcgcatccat gatggttgca atggattctt cttttgcctc tttgataaat
     4801 ccgcaggtat tgataaaaat aatgttggca tcatccatgc catgagctgg aacaaaccct
     4861 gcattatgca aatcagcatt gagccgctct gcatcaacct cattttttgg gcatcctaat
     4921 gaaatgatat aatatgaaag ttcctgcggc aaaaacacat caccaatctt taacttctaa
     4981 tttatattta ttaggattgg ttgggtcctt ccgccaggta ataaccttat ttgctacctg
     5041 ccctggcaag ccaaatacat aatcaacatt gtttatttta actttgaccc caccagcatt
     5101 gcctatcttt atctgaatat attctgaagc ttcccatcgc tctttgtttc ctgctggtat
     5161 aaagcccctg accttttcat taccatcaag atataattca aaatatgttt tttgaataaa
     5221 ttcagtttca agtactatct tcaaattctt ctgatcatgg gttaccacct cggtccccgg
     5281 tgtaaccggt gttactacac ttaccgcttc ttcctgttca actgcttgct gtgcagcagt
     5341 aagctgaacc ttaatctttg ctctgttttc ggttaatcct ttcaatgtta aaattatatt
     5401 ctgtgatgac tccataggac tgcacacaac cgacttttcc atctccagta tatacttctt
     5461 tgaatcaggt aggaactcaa ctacaacaga atcgtctttt atttcattta ataaaaacag
     5521 tatttcttta ttttcaacta aaaactggca cgcttcattg ttatacagca aaacaatacc
     5581 gctatcattg acaatctgta acggccatat attcttgatg gcaatctttt taccctgtgt
     5641 gcggtaatat tcttcttgta ttgcttttat agaatcatcg ctgccaatat ggacatcaga
     5701 tgtaataagg cttataaata gtgcaataac aataagtacc gctaccccaa taccagcaat
     5761 atataaaata tttttatact gctcggcata catgcgtagc tgagtaaaaa ttgaaagccc
     5821 ggttggcctt gttaactctt ctaatggagt ggaactctca ccaattttat accctttata
     5881 cgcctgtatc agctcatcag catccagccg cagatattca gcatatgaac gtaaaaaacc
     5941 caatagatat gtttcactgg ggaatttatc aaattcttca ttttcaagag cttccagata
     6001 tttaacaact atgtttgtat cttttgaaac atctttcaat gttagccttt ttgcttcgcg
     6061 agcattgcgt aatgtttcgc caatttctgc caccatatgc tattcctcta ctaacatagg
     6121 atttttaatt attcgggcat ttgaaggagg atcaaactta aaaaatcctt tttttacatc
     6181 tatttttgta gtaacatttt ttatttccag tgttatattt tttccgctgg ctgtttcacc
     6241 atatgccttt gtaataaaat actgctcatt aacccatagt tttaatgttc tgaatccgct
     6301 ccgtgtttct ttttgtttta aaaataatac ataatatcta ttattacttt cctcacgagg
     6361 ctgttctttc ccatcaaacc ggtaatggta ctttgaaaag agcctgttta aaccaggtcc
     6421 ggtaccaact gaaaaaagtg tacccgagtt taaatcctgt tctgcaacaa tgttcaatga
     6481 agggatatat atccatacct ggttcccatc agatacaatt cgctgtccat agggagaatc
     6541 aaaatcaaca cgcaggtaat gaggagattt ataccatatt gttccactct gagtctgacg
     6601 cttcccaata cggtcagaaa tcataacaaa gcttgcctga taactatcaa catcagagaa
     6661 tgcctttttt atctgcttaa caatatcatt gactgtgata aagtcaaatt tatacggata
     6721 ccccattatg cagggtacaa aaattaaaag aactatcgcc aaaattataa aatttttctt
     6781 ttttgctatt gacataccag cctttaagtg cctttttaga tatataagga taaaccaaca
     6841 tattttttat aataaatatg actatgtcaa cctaaaaagc ttttttaaaa agtcaataca
     6901 aaaaagcaaa aagattgctg ggtgtaaaaa ttataatttt tttatgtaga caaattttta
     6961 tcatgccttt actatttaag tgatgctacc ataaggagta aactatgggt tccataaaac
     7021 aacctgaaat acagcaagag cagcaacatc cagaaacaat tgtttcgcaa tggaataatt
     7081 ttaaattgct ggaatcacag caaatttatt tttctgttgg gaaagctacc ataaagcata
     7141 tactaataaa gcgagaacca cttattggag catgagattt actatggtca ataaacggtt
     7201 tgtcatagtt ttcactgtag tgttattgtc agtgcagcca ttacattcac acgttgcaac
     7261 cagcagtatc attgaggaaa atagagattt ctggaatcag cttattcaaa caataactat
     7321 cgccaataaa aatttattgt atgcaagtta tgtagcctat aaagataagt tatatgacct
     7381 gtccatagat tcttttcagg aatgtattgc gcaaaaccct gataatcaac tgatacaggg
     7441 agttgccaat tattatattg gtaaaaattt ttatcacatt ggtgaatata aaaaagcggc
     7501 gctgtttttt ttagcaaccg aacgatacag catgaatcag tattcctcct taaaggctgc
     7561 agcattgatc aatgctgctt tatgttatat tgcaattgat gatatagcgt tagcaaaaac
     7621 atatctgcaa aaatcattac agtatgatat tgaaggcacc taccgaacaa ctgctgatag
     7681 tcttttgcaa tccataaaag aacattaaac agtataaaac tttttatacc attgaacaaa
     7741 caatgacaat ccctgttcaa ttgaagttac agggctgtaa ccagtggctt gtgttaactc
     7801 atctatatcg gcaaacgttg caagcacatc acccggctgc atgggcaaaa aatgtattgt
     7861 tgctttttta cctatttctt tttccagtac ctcaatgaaa tacagcagtg aaaccggctt
     7921 gttattgccg atgttataaa tccggtacgg cgctgtgctt gatgccggat cagggtgcat
     7981 ggtgtcaaaa tgaggtttcc cctgtggtac gataaaggtt attctgtaaa caccctcaac
     8041 aatatcatca atataggtaa aatcccgctc catatctcca tagttataca cattgatggg
     8101 cttatcctca acaatagatt ttgtgaaggt aaagtatgcc atatccggac gcccccatgg
     8161 tccataaacg gtaaaaaacc tgagaccact ggtaggaatt ccatataaat gcgaataaca
     8221 gtgagcaatc aattcatccg attttttagt agcagcatac agcgatacag gatgatcaac
     8281 agtatcatgt actgaaaatg gcacgcatga attaagtccg tatacggaac ttgacgatgc
     8341 ataaactaga tgattcacag gattatgtcg gcaacattcc acaatattca taaagccaac
     8401 gatattgctc tgtatatact caaagggatg gtcaaggcta tagcgcaccc ctgcctgtgc
     8461 agcaaggtga acaacatagt caggggtatg ttttttaaat atgtgcgata gttcctcata
     8521 attagctata tcgcattgat aaaatatata gtttttatca ttgagcagca gcgcatttcg
     8581 tgcttccttt aatgaaacat cgtagtagct attgatagca tcaacaccaa taaccgtata
     8641 gccctgttgt aaaaacttca gcgctgtata ataaccaata aaaccagcag aacctgtaac
     8701 aagtatattc attgcatcac accgtaacat ttaaaaaata ttttttatat aatcgctttc
     8761 cccatcccac actctaaagt ctggatttcc tgcatcagtt tttttggtaa tgtggttatt
     8821 aaaacagcag tatagccaat tgatacagaa atatcataaa ctaattttgc cagatactga
     8881 taataacttt cttcccgggc atcacctttt aaaaaggtag tatatacgtt ttcattatat
     8941 tcatgaatac tatcatgtat cataaaaata ccagttagtt aattatagtt ggtaacaata
     9001 caagcaacta taatgcaaat cttcatttta tatgtcaaag cttatatggt gtaatttttc
     9061 ttttcagtac agataatcaa ggattttttt attgcatcat gaaaaattaa tagtctattt
     9121 ttttatttac ctgctttttt tgagcaacta caaatagtag ctattacaat aattaccaga
     9181 acaaaaaagc aatatcaggt attattgatg tcatcatcag gtttgagtgc acctttccca
     9241 aataacctca cagcctggta aacataccat gctctgaatt tgctcatgcc atcttcaata
     9301 caaatatcgt gtaaaagttt atccgcataa tctttatacg atacaggaag ttgtttcttg
     9361 cgcatcagtt gatatagcgc atcatggact aaacttcctc gcataaaatc ttttgtatca
     9421 atggttggcc cgcttggccc gtcccatgca taccctttaa agatatacaa ctgcccgtct
     9481 ttttttaatg caacaaaatc atcaatgcag cattcctgct gtaatgtaat tgatgtagca
     9541 tgtatatagt tttccataag ttgatattta taatgggtaa gttttctaaa gtgtattgta
     9601 aattttttag acatacatta ttccttaaag aataaatcta taaaaaccta tccggcagtt
     9661 taccgccatt acattattcc acacactata caacgatgag tccagtaata tcttcattca
     9721 taatgcataa tggttatggc agtgcacctt ttaatcttgt agaaaactaa aattggtttc
     9781 tatcacacta tattttatag ttatgaaaag gatgatacac tatttttcaa tatctacgcc
     9841 ttagtgataa aatttagaac agcaggataa atctgaataa acaattcttt accagcgtaa
     9901 caaattgttt cgccatcaca atgaacaggt agatttccct tctcagcaac aactgcaatt
     9961 tttgtagttc tatcctccaa tacttctttg cgtttaccct gagtaccttt tataaaatat
    10021 gggatcatac tgaaaatctt aaactgattc atcgatctaa ccatgcaaac gtcaagcaaa
    10081 ccatcaaaag gactaccatt aggagcaaaa tggaaacctc ctcccatacg cttcccattc
    10141 ataatagaaa tctgtaatgc ttccattatt gtttctttat cattgtacga taatctaatc
    10201 ctgggagcat tatagtaaat aaatattgtt tgtaaagcag caatgaaata caccagcata
    10261 ccgctcaaaa atctgattct acttgccaca aaaccagtgg ttgcatcaaa tccaattcca
    10321 ataccattcc caaaatatct tccctgtgga taatcacccc cctttataaa accaacatca
    10381 attttgcgta gtgtataatc agctattacc tttatacatt catcgatatc atcagataat
    10441 cccattccat aagcaaaatc attaccggtt cccacaggta tctggcccat tgcagtttta
    10501 ttatttcctt ttttccgagc aagcattaaa ccatttaata cctcatttgc agttccatca
    10561 ccgcttgcaa caactaccgc tttatatcct ttttttgcag catcctctgc cagttcagca
    10621 gcatgccatt gtcgtatggt aaatacaata tcatacttaa tacccagacg attaaaatct
    10681 ctttcaattt gcggtacaga ttttgcagcc cgacctcttc cagcaattgg attaactatt
    10741 aacaaaattt tatcattcat tataactatc gcccctatag gtttacaggt ataaaaattg
    10801 ctgtggagta ttaaacagtt aataaatttt gtcaataata aaacgaggtt acagggaaag
    10861 gctgtttttt atagcggtaa aaagctcatc taaatcaaat ggttttaaca aaacataatc
    10921 cacaatgcct acataattat tattcattgc cagatagtta cctgaaacaa tgatgatatt
    10981 gggacggttt gcaatatatt gaattttttt taatatatcc catacactca tatcaggcag
    11041 gtgaacatcg agcaatacca gatcagcacc atgtgcatca agaaagttaa gcccctcagc
    11101 tccggagttt gttataaata cttcggcatt tttagcatgc aatgctcttt gtatgattgt
    11161 agcaaattca acatcatctt ctattatcaa tatttttttc ttcaaaaaat tatagctatt
    11221 ataatttacc gctatttcag aatctacttt ttcagtggta ttaagcggta ataaaagctt
    11281 aaatgcagtc ccttcacccg gagtgctttc aacatgtatt gctccattat gttcttccac
    11341 tataccaagc gctacagaaa gtccaagccc cgtacctgga atgtttgaat gccccagagg
    11401 acctttcgta gtaaaaaaag gttcaaaaat tctttgcaaa tattccgctt ttataccaca
    11461 tcccgtatcc cttatctcta tatatatatt ttctttatcc tgataggttg taattgacaa
    11521 ctgttttttt gcagaatcaa tcatcgcatc acgggcattg agaagcaaat tcatgagcgc
    11581 aagggtaatt ttattagcat ctaaaaacag ttccgatagt ttatttttat ttataacctc
    11641 aatagaaatc ccggcatcaa taaactgagg ttttataata ctaacgcaat cattaacaat
    11701 gtcttctata tttgagagca tttgtttaga aggcttttta tgagccagca tggaaagctg
    11761 atcaaccaga tgtgttgcac gcttaacaat gcgcaaaact gttctaaggt ctttaatccc
    11821 agcatttgat agttcttcct gtgacaataa cagttctatt aacccttgaa taccagcatt
    11881 catattatta aactcatgag ctatcccgga cgccaatgtt gagattgtca tgtttttttc
    11941 cagcatcacc agattttgac ggttctgtat atcgctatcg gtcacaatat gaagaaatac
    12001 cagatcatct tctttttttg ctggtgcaat tatgatatcc atctgctctt tgacagtgtg
    12061 tacaatcatt gaaagctttc gtttttcaca atcggtaggt attttataat ctgcatcatc
    12121 aacaggacaa ttcccatcct tttcacaatc cgggaaaagt tctttacatg attttcctac
    12181 tatatccata gtatgaaaca gccgtcgggc atgattattg atgtcaatga tgtgttttga
    12241 tgcatcaacc accaaaacag gcgcctgaat cgaatccatc aaacacctat aatccaactt
    12301 tattttatca ttcatatata attattattg tatataatat atttgtatgt aaagtcaatt
    12361 aataatcaaa tttttatata taattctact aataaaaata gtacactgtg agtatattat
    12421 caaaatttta atggtgaaaa tacaaatatt aataaaacaa aacaactgaa atgttattat
    12481 actttttagt atatcaattt tttcataatt attgtccatt tacctctgtt ttccccttta
    12541 tatatgatat agtatatagt ataaacgtta tgaatacctt tgataacact ataactgttg
    12601 ggatagttgg tgcgggtgcc atgggctctc tgtttgcttt tctgctgcat ccctatgcaa
    12661 cagtagtaat gtatgaaaca aacaaaacaa cagtaaacga tattaaaaaa aatgggcttt
    12721 caatagaata taatacacgg acacatgcca ttaatcctat gataagcaac aatcccggca
    12781 tacttgcaca tgctgattgt atatttattt ttgttaaaag ctttgcaact aaaagtgcca
    12841 ttgaagcaat aaaaccttat ataaaatctg atgcaataat cataacatta caaaatggaa
    12901 ttggcaatgt ccctgtaata caatcaatgg taaacaatcc gcttgtatat ggtactacca
    12961 ccatcggtgc caccaggctt actgcagcaa aggtacgttt tggtggtgaa ggacaaataa
    13021 ttataggtgg cgatagttat cgtcataaca gctttctgta ttcattgtta cagaaggctg
    13081 gattacaggt aactatcact caaaatcctg ataaagcact gtgggaaaaa gccattgtta
    13141 atgctgcgat taatccatta ggtgcaatcc ttgaagtgcc caatggagct ttactgtacc
    13201 atgactatat aaaagatatt atgcgcaaca ttattatgga agcagcagat gttgggctgg
    13261 cacaggggat tgctatacat ggcaatgata tgattacaac cacatttcag gtatgcacca
    13321 acacaaagaa caactattgt tctatgcttc aggatattag tgcaaaacgt attaccgaga
    13381 ttgattatat taatggtgca attataaaaa cagccatgca ttatggtatt gcaacaccgt
    13441 ataatgcaac agtatatcgt ttagtaaaat ataaagaatt actgctgtaa tgcaatcata
    13501 ctacggcaca ttcaccatag agcattgctt gatgcgtacc taacatcctg atagtattgc
    13561 tatctgttcc gctatcgctt ttattgcttc tttggcgggt agcattgtta tcttttcccc
    13621 ttttgcaaaa agcaccaccc tgccattacg agcccctgca attcccacat ctgcatgacg
    13681 tgcttcaccg gggccattaa cctcacaacc cataatagcc accgttatat gatgcaattt
    13741 ttttttcagt atctcttcaa agcggtcatg catcatctgt gttacttcct gcgccagccc
    13801 taatatatca agctgttcat ccgttcgtcc acatgttgga cacgcaataa tggtaaatgg
    13861 gctgaacctg aatccaccta tctctaatag gcgttttgca atccttacct cgtggatagg
    13921 gtcccccgtc atggaaatcc gaatggtgtt gcctatgccc tcatacagca gtgttcctat
    13981 taccatactg cttgcaatgg tgcaatcggt gccatatcct gcttcggtaa gcccaatatg
    14041 gatggggtac tgtcgcctgc tggcaaatat tcggttagcc tcaattgtct gtaataaatc
    14101 tgacgatttg atggaaacaa caatagtggt ataattgcaa tcctctaaaa tatgtacatg
    14161 ctccattgcg gaagcaacca gcgtatcagg attaacctgc ccatattttt ttaagtccac
    14221 cgaacctgag ttaacgccaa tgcgtatggg aatatttttt tcacgggcag ccttcacaag
    14281 ctctataacg ttttcttttg caccaatatt tcccggattt atacgcagct tatcaatgcc
    14341 acattctata gctttgattg ctattttata attaaagtgg atgtccgcac acagtggcac
    14401 attcacttcg tgttttattt ggctgatata ctttgcatcc tcttcggtgc gcatcgccaa
    14461 tcgtacaagc tgtgcccctt cctgctcaag ctttataatc tgctcaattg tttttgcaac
    14521 attgctaacg gggatattgg tcattgattg caccgataca ggagcatccc cgccaatggc
    14581 aagattccct gcatggacgg ttattgtctt tttgggagta gtgctcatgt accagtaact
    14641 atcgccctaa aataaatctt tgaaaaaagg taaaaatgac agatcattga atatgacaaa
    14701 tatacttaat aataccaata ttgcaaaacc aatcatctgt attttttcta aaagcttttg
    14761 attaaatgcg cgccgcgcaa ctgcctcgta aagaaaaata agtatgtagc ttccatcaac
    14821 catgggaatg ggaagcaaat tcattaccat gagaataata gaaatttttg ccatcaaaac
    14881 aacaaatgcg cttaccccgc gataatatgc ggtatcacct gcaatctttg caatgcgtat
    14941 tgggccggat aaattttgac gaacatcaag ctcgcccgaa aacatcattc caatgccccg
    15001 aaaattaaga gcaataaaat caaacgggtc aaccatggat ttaacaaacg catccgttat
    15061 ggtatattgt ataaacttat tttccggaga tattgccgtt tctattccaa caaagccaaa
    15121 gctttcaaat ttcattatcc ccctatagtt gccacctggc gtctgaagcg ttaccacccc
    15181 gtttgtggat gctataattt ttttcatctg cgtaaaggat gtcaccgtat atccgtttac
    15241 ctttaccctg cccttatctg ccgcagcttt tataagtgac agtgaatcaa ctgttataga
    15301 gtgtttttct tttggaaagc gcaagtcggt aaaatcagta atagtaagca catccttttg
    15361 aatgggggtc accggaacgg tcataatggt accgccacgg ttaatggtaa gcgatagttt
    15421 ctttaaagga ttttttcgta caaatgaagt aaactcctcc ggagaatgga actgtgtgcc
    15481 attagcctca ataatttcat caagttcctt caatcctgcc ttttgagcag gcgaattttc
    15541 cagtatgccc atgacaagaa ccctgttgcc atagggcata acaccaatgg tatagtatcc
    15601 ttttttggtg tatttttttg gagtgatagt tacctcaaat gaatcgttgc ctctgagcgc
    15661 ttttataaca attggtctgc cctttgagaa tgcaatttta ctctgaatat ctataaaact
    15721 gatgacctgc tcattattaa ttgctacgat tatatcccct gttcgcagtc cagcggtata
    15781 agcgggcgat acatattcac cttttgttaa atattccgga atatatatct tgtttgtttc
    15841 aaccggatag ccataaacat tcatcacaaa aaatagtata atgccaaaaa aaagattgaa
    15901 gagtggtccc atcactacag taaccacccg tttcaaggga tgagcactta aaaactcgta
    15961 cgatttacct tcacgagctt ctgtcgggtc ctcaccataa aatttacaat agccaccaaa
    16021 cggtataagt gttatttgat aggttgtatc gccccatttt ttctttatga atcctttgcc
    16081 ataacctata gaaaaaacct cagctttaat accaacaagt tttcctccta aaagatgtcc
    16141 cagttcatgt acaaagatac ataaaccaag aagcactact gctgccagaa tatacgtgat
    16201 tgctatcatt atttacctca tatacattgt aatgcaaatt gccgcgcctg ctcatcggca
    16261 tcaaatatca tgcccaaatc aggattttca atacagttat gtttttgcaa aaccttttct
    16321 acaaccttaa caatttttgt aaatgtaatt ttcttctgta aaaatgcctg tactgcaact
    16381 tcattagctg cgtttaacac tgcaggcata gtcccaccca tttttccggc ttcataacac
    16441 aatgccagag caggataccg tgccatatca ggtttagaaa aatgtaaaga acccattaac
    16501 gttagatcaa cattgccaaa tgggttatgg catatatccg gataagttaa tgccctttga
    16561 atagggtaag ccatatcggt aatactcata tgagcaagca atgtcccatc attcaattca
    16621 accataccat ggacaatact ttcagggtgt atgatgactt caattgcatt atagggtaca
    16681 ttaaaaagat aatgtgcttc tattacctca agccctttat tcatcatggt tgccgaatca
    16741 atggtaatct ttttgcccat atcccatgtg gggtgcatca gcgcttcttc aacagtaaca
    16801 aaaccaagcg tttccaccgc tttatgccgc aggctcccac cggaagcggt aagtattata
    16861 cgctgaatgg tattaacagg tttaccatct atcagggtaa atatagcaca atgctcactg
    16921 tcaacaggca aaagttcaac attatagtgt tgcaaaaaat gtaaaaagat gtcccctgcc
    16981 ataaccagcg tttctttgtt agcaatggct attcgtttga tatgaggtag ggcatttatt
    17041 gaaggcttta gtgcagctcc accaacaatc cctacaacta aaatatcagc atcattacta
    17101 acaagctgat ttaagccatc catcccttca taaaaaacta tcgaacaaaa gtgtttctta
    17161 agttgtttat agtcctgtgt taaacaaacc tgttcatcgc taacggcaac aaagtcagga
    17221 tgaaactctt taatctgttc tttgagcaac gtaatatttg tatggcacga taatccctta
    17281 acaacaaatt gattgttaaa ctcacggatt acttttaatg tagaaatgcc aatagacccg
    17341 gtagaaccca atataataat ttgttttgac atacaactat ggatgcaacc ttatattatt
    17401 ccccgcagta tcaggtaata gtaaaacaat ggcattgaaa aaattgttga atcaaagaca
    17461 tcccacatgc cgccatgtcc aggaataatt gagccagaat cttttattga gccatcacgt
    17521 tttaacgctg attcaagaag atcgcctata ttgcctataa tactgaatat aatcccaatg
    17581 attgcagcct cgataatact aaataaatat cttccgtaaa atatttcaat aaactggcta
    17641 tataaaattg caaagacaat gccaaacaaa agcccgctaa aatatccttc ccatgattta
    17701 tttggtgatg ctttaaattg agccttatgt tttccaaaat aaactccacc aaaatatgca
    17761 ccactatcat tgatcataac aatcaaatga ataaatatga taaatataac gccatcacgt
    17821 attgatttaa gaagcataat atgtgcataa ggcaaaacaa taataaacca gcccagtata
    17881 gtagcgccta tggaataaat accacccttc aagggccgtt taaaaatatg aatggttgcc
    17941 agtacgctca cagctatcgt tacaacagcc agtaaaaagc ggacatcaaa attaagtgtt
    18001 atgccaccaa tacccgttat attgccatac gcatacgaat acataaccat atttatgata
    18061 acaccaaaaa tgatacctgt cttaacaaat ggtttatagc ctgtagtgcc ttctgctatc
    18121 tgataaaatt cccataaaca aacaccagac acaatcacac ttactataag gataggcaac
    18181 gattgaagca tattggtaat aattaaaaaa acatatacag gcagtgcaac aacagcagta
    18241 aggatacgaa tcattgtatt ttttgtgcgc tctctcataa tccgccaaac ctcctttcac
    18301 ggtgttgata ttcatagata gcctgatata gatgttgttc attaaaatca ggccataata
    18361 ctttcataaa aatcagttca gcatatgcta tctgccatag caaaaagttg ctaattctgt
    18421 actcgccact tgtccttatt aaaaggtcaa cattgggtaa gccagcagtg tataaataat
    18481 tttcaaaaat ttcaggggta agcgatagct catgtgaagg tctttgttgc aaccaattat
    18541 taaccgcatg tataatctca tgcctgccac catagtttaa acaaaaattt aacacaatag
    18601 tagtgttatt ttttgattta tccaccgcat tggcaataat acgctgaatg gtgggtggca
    18661 attcactcaa gctgccagaa tgttgaatcc ttacaccttt ttcaataata gtagaaagct
    18721 tcttttcaaa aaaagattct aaaagtttcc ataagccatt gatctcttct ttaggacgtg
    18781 cccagttctc cgttgaaaat gcataaagcg aaacaacacc aatccccaat tcaatagctg
    18841 aatccataca ccgctcaata acctcagcgc ccctccgatg cccttcaatt cgaggcaggc
    18901 ctcttttttt tgcccatctg ccattgccat ccataatgat agctatgtgc tttgggattt
    18961 tagcataatc aattttcttt ttatagttag caaacataat atatagtata acatagttta
    19021 aaaatactta caagctcata atctcttttt ctttattatc tgtcatcatc tgcacttcac
    19081 caacaaactt atcggttaac ttttgaactt catccagtgc attctttgct tcgtcctcgg
    19141 aaatatcttt tgatttttca agatctttaa tcatatcatt accgtcacgt ctgatatttc
    19201 gtattgcaac cttgcagtcc tcagcctttt gttttatcat cttaacatat tcttttcttc
    19261 tttcttcggt aagttcagga atctgaatgc gtataatatt gccatcatta ttaggtgtta
    19321 atgaaagatt tgattttaat atagcttttt ctattgctcc taaatttgtt ctatcccagg
    19381 gttgaatcac taccaatcgt ggttcagggc atgaaatggt tgctacttga ttcaatggca
    19441 tttcagtacc atacgtttca acctttacat tatcaaatat cgctggatta gctcttcccg
    19501 ttcgtattgt ggtaaaatct tttgccagag cctgaacact tttttgcatg cgatttttta
    19561 catcatcaag aatatccttt atcatacaca ttcctccnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnncag cgatccttca tcaaacaggt taaataccac aagcggcaat
    19741 ttattttcca tgcataatga aacggcagtg taatccatta cacctaactg ctgtttaatt
    19801 acatccatat aggatactgt attcatttta tttgcttcag gaaatttgac aggatctttg
    19861 tcgtataccc catcaacttt agtagcttta caaagaagat cagcacctat ttcaatagct
    19921 ctgagtgctg ctgctgtatc agtagtaaaa aatggattgc cggtacctgc tgcaaaaata
    19981 acaattcttc ttttttcaag atggcgtaat gctcttctcc gtatatatgg ctcggctacc
    20041 tggcgcatct caattgctgt cataacacgg gttggtattt gcatattttc aagtacactc
    20101 tggactgcca gtgcattgat aatagttcca agcatcccca tataatcacc ggtggctcgt
    20161 tcaatgccta ttgatgtacc aactgaacct ctgaaaatat taccgccacc aatgacaagc
    20221 gcaatatcaa ctcccatagc gtaggctttt ttaacctcac gggcaatatg ggaaacaatc
    20281 tcaggattga ttcccacttt attttgtcct gcaagcgcct caccgctgag ttttaataaa
    20341 attcgtttat actttagctg tgattcttct tccatcctgt taacctatag taaggcaacg
    20401 taacaaatat gcataactat cgctgcccta tctggaaacg aacaaaacga ccaacagtta
    20461 tattttcgcc ataggtagca atcttatttt tgataaggtc cttaataatg accttattgt
    20521 ctttaataaa ttcctgctct aaaaggcata cttcagaata aaacttttgt aattttcctt
    20581 caacaatttt ttctataaca tttgctggtt taccactttc tttaagttgt tccctgtata
    20641 tttccttctc acgctccagt acatcagcag caacatcttc agggcttaca tataagggat
    20701 ttgcagcagc aacctgcata cataaatcct ttgccagatt ctgaaaatcc tgattacgtg
    20761 caacaaaatc agtttcacag ttaagctcta aaagcacacc aattttactg ttattatgaa
    20821 tgtaggaagc aataatcccc tcttttgcaa tacgaccttc gcgcttattg gcagcagcaa
    20881 gccctttttt gcgcaaataa tcaactgctg cttccatatc gccgccgcat tctatcaatg
    20941 cttttttaca atcaagcata cctgcctgag ttttttcccg taattctttt atcatgtcac
    21001 ttgtaatatc agccacatta ctactcctta tacactataa aataatatta ttacattcac
    21061 aggctaccgt ctgtcatctt catacacatc aatttcatca gcatattcct cagcataatc
    21121 tttctcaggt tttgcttcgg attgctcttc ctcaacaaag tcaatggtag ctgtcgcagc
    21181 ttcgccagtt acctgtcctg ccgattgccc ctcaataatt gcattagaca tcacctctaa
    21241 aaataatgct attgccctta ttgcgtcatc attgccaggg attgggtaat ctatttcatc
    21301 agggttacag ttggtatcaa cgacagaaaa aataggaata cccagctttc gtgcctcctg
    21361 aacagcaata gcttcacgtt tgggatcaat aatgaataat gcttcaggca atttgtccat
    21421 gtccttgata ccttcaagta ctttgcgctt gcgttccaat tcgcgcgtca gctcaaggat
    21481 ttctttttta gtttcagcat cccactttcc tgttatttcc atttgctcca gttctttcaa
    21541 tcgttggatg gaacgtgaaa cagttttgaa attagttaac aatccaccca accatcgttc
    21601 agatacataa aattgtccgc atttctgcgc atattccttt actgcagcct gagcctgttt
    21661 ttttgtgcca acaaaaagta ttctgccacc tttggctgca atttctttca tcgctgcata
    21721 tgcaagctta agacgctgca ttgttttttg caaatcgata atgtggatac cattacgcgc
    21781 agtaaaaata aactgtgcca ttcggggatt ccatcttctt gtctgatgac caaaatgaac
    21841 ccctgattcc aatagtgctt tcattgatat aactgacaat gaaatacctc ctcatataag
    21901 attcaatgtt aaaaaacaca tttattttac tatcaaataa aca
//
LOCUS       DAOSOX010000020        21620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2421_length_21620_cov_8.569859, whole genome shotgun sequence.
ACCESSION   DAOSOX010000020 DAOSOX010000000
VERSION     DAOSOX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21620
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_21620_cov_8.569859"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3111
                     /gene="rpoC"
                     /locus_tag="PLX22_02775"
     CDS             <1..3111
                     /gene="rpoC"
                     /locus_tag="PLX22_02775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT18860.1"
                     /translation="LFKPFIMKRLVDKGFVQNIKSAKKMVENERPEVWEVLEEVISEH
                     PVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCHAYNADFDGDQMAVHVPLSPRAQ
                     IECWILMLSANNLLNPANGQPIVTPTQDIVLGVYYLTSELKSKSEHLPYFGSMDEILY
                     ALEYKQIKYRSRIKYLKDGEFIETTPGRLIFNEKLPAGFPFVNYTINDKELSKIISKI
                     FRGYGPTATVKVLDDVKELGYRFATYFGPTISTSDVKVPEMKDELIKKANEMLEKIEN
                     EYRDGWRTNEERYNRVIDLWTKTNEDVTEVMFDTMAQDADGFNPIYIMAQSGARGSKQ
                     QIRQLAGMRGLMAKPSGEIIDVPIKSNFKEGLTVLEYFISTNGARKGLADTALKTADA
                     GYLTRRLVDIAQDVVITMDDCGTSVGIDVTAIKEGDEVIESIGSRALGRTLLYDLYHP
                     VTNESICNSGELITEDIAEEIDELGLDRITIRSALTCEARHGVCARCYGRNLATSRMV
                     DIGEAVGIIAAQSIGQPGTQLTMRTFHIGGTASHFVEESEISFNYPVYVKETTFKTVK
                     TEDEKGEKKTISVRRGYIIIQRLLEEISAKNGDILVNNGDRVYSGTPVVKKPDGSFIT
                     TSKAGIVKLDKSRIYILGEQNMIPVNIGSTIYCKTDKIYPVDPQTGKTEMLVGFDPWF
                     EPILTEINGKVKFIDIEINKSMREEIDPLTHQLKRIIVETKTEKLQPAIEVSSAEELF
                     HYLLPKGAHLFVDDGMSVKAGTVLAKIPKEVEKTKDITGGLPRVAELFEARTPKEKAT
                     ISEIDGYVKIGDTVKNKRKIIITAEDGDFREYQIPASKYLRVQDADWINKGDKIDDGP
                     IDPHDILDIKGPRELEQFLVNEIQEVYRLQGVQINDKHIEIIVRQMLRKVIIEDPGDT
                     SFVVDQIVDKFEFDDENIRVEKEGGKAASAKPVLMGITKSALNTESFISAASFQETTR
                     VLTEAAIKGKIDRLRGLKENVIIGHLIPAGTGVRDYNKVTVYQKIPGDLDGVVKEIEE
                     EISVEK"
     gene            3225..3599
                     /gene="rpsL"
                     /locus_tag="PLX22_02780"
     CDS             3225..3599
                     /gene="rpsL"
                     /locus_tag="PLX22_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT18861.1"
                     /translation="MPTINQLIRKGRSEKVKKSKSPALKSCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDLPGVRYHIVRGTL
                     DSLGVNDRKKGRSKYGTKRPKA"
     gene            3692..4162
                     /gene="rpsG"
                     /locus_tag="PLX22_02785"
     CDS             3692..4162
                     /gene="rpsG"
                     /locus_tag="PLX22_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT18862.1"
                     /translation="MARRRRPAKRTILPDPKYGNIVVSKFINSMMTEGKKGVAERAFY
                     KALELIGQRTQKDPIEVFINSLENVKPLVEVKSRRVGGATYQVPVEIRPERRQALAIR
                     WIVTHSRNRSEKTMFQKLAGELLDAFNNTGTSIKKKEDTHKMAEANKAFAHYKW"
     gene            4286..6364
                     /gene="fusA"
                     /locus_tag="PLX22_02790"
     CDS             4286..6364
                     /gene="fusA"
                     /locus_tag="PLX22_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT18863.1"
                     /translation="MGRELPIHRTRNIGIMAHIDAGKTTLTERILYYTGKTYKIGEVH
                     DGTAEMDWMIQEKERGITITAAATTTYWKNTRINIIDTPGHVDFTVEVERSLRVLDGA
                     IAVFDAVAGVEPQSETVWRQADHYNVPRICFINKMDRVGADFERCLTMIKERLYSIPL
                     PLQIPYGKEDIFRGVIDLIQMKGIIWTDASGEHYEYTAIPDELQSEAQYYREHLIETV
                     AEHDEQILDKYLAGVEIDEQELKRAIRTVTIKSALFPVFCGSALKNKGVQPLIDAIVD
                     YLPSPKDIPSIKGNDAHHDDVIVERHPDDKEPFCALCFKIMTDAYVGKLCYVRVYSGH
                     VSVGESILNVNKQKKERIQRILRMHANDREDIKELYTGDIAAIVGFKFAQTGDTITSI
                     DAPIVLGKMEFPEPVISIAIEPKTRADLERLHMILKRLVDEDPTIFVKVDEESGQTVL
                     SGMGELHLEIIVDRLQREFNLPVNIGKPQVAYKETVSTTAKAEGIFEKEIGGKLQYGH
                     VLLEVSPCDSHFEFISKLDSAAIPDIIIKAIEMGCRDALNSGVIAGYEMTRIRVIVED
                     AKYREDVSVEHAYRIATTMAFRDAVKKATPVLLEPIMKLEAVVPDEYMGDIINDINAR
                     RGKIEHIDIRGMLKVIDAYVPLSEAFGYATAVRSLSQGRASHTLQFNHYDVVPQNVME
                     KIIGSYYRPV"
     gene            6659..7873
                     /gene="tuf"
                     /locus_tag="PLX22_02795"
     CDS             6659..7873
                     /gene="tuf"
                     /locus_tag="PLX22_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT18864.1"
                     /translation="MAKAKFERTKPHVNVGTIGHIDHGKTTLTSAITKVLTAAYGGHN
                     KPIDFDQIDNAPEEKARGITIATSHQEYETPNRHYAHVDCPGHADYIKNMITGAAQMD
                     VAILVVAATDGPMPQTKEHVLLARQVNVPYIIVFLNKVDMLDPADRDELVELVEMEIR
                     DLLNKYEFPGDTIPIIPGSALKAMQCGCGKADCEACGPILKLAETLDTYVPEPKRDKD
                     KPFLMPIEDVFSITGRGTVVTGRIERGMVRTGDEVEIVGFGETKKSVVTGVEMFRKIL
                     DEGMAGDNVGCLLRGIGKEEVERGQVLAKPGSITPHRKFEAEVYVLSKEEGGRHTPFF
                     TNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIKMTVELITPVAMEETLRFAIREGGR
                     TVGAGVVAKILE"
     gene            8052..8369
                     /gene="rpsJ"
                     /locus_tag="PLX22_02800"
     CDS             8052..8369
                     /gene="rpsJ"
                     /locus_tag="PLX22_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT18865.1"
                     /translation="MAKLAGQRIRVKLRSFDPYLLDQSAAKIVATTNRSGAKVAGPIP
                     LPTRIEKFCVLRSPHVNKKSREQFEIRTHKRLIDIIDPNSDTVEALMKLELPAGVSVD
                     IKS"
     gene            8451..9068
                     /gene="rplC"
                     /locus_tag="PLX22_02805"
     CDS             8451..9068
                     /gene="rplC"
                     /locus_tag="PLX22_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT18866.1"
                     /translation="MKKALIGRKIGMSQLFLDDGTVIPVTVCELGPCVVVQKKTIEKD
                     GYNALKLGYWETKPHRLTKPIIEDLKKKNLPIVKVFKEVDIFDEDLIEGSIITCDVFS
                     ENQIVDVSGTSKGRGFAGVIKRYGFHGGKETHGSNFHRAPGSIGANTYPAEVWKGKKL
                     PGRHGGKTVTVKNLKIIRIMKDKNLVIISGAIPGTVNSIITVQSK"
     gene            9080..9706
                     /gene="rplD"
                     /locus_tag="PLX22_02810"
     CDS             9080..9706
                     /gene="rplD"
                     /locus_tag="PLX22_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT18867.1"
                     /translation="MVVDVYTLEGNIKGQIELNESIFNAKVNDVLMYELIKAANANLR
                     QGTHDTKERSYVSGGGAKPWRQKGTGRARQGSIRAPQWKGGGAVFGPHPRDYRIELPK
                     RIKHEAYRSLLSMKFKEGSIKIIEDFKVVDGKTKEVAAIAKKLNISKGVLITDIEDNL
                     LKRAIRNIPWFLYNNVNRLSGRDIFYTKTVLITENAAKIINSKYAKGE"
     gene            9709..9993
                     /gene="rplW"
                     /locus_tag="PLX22_02815"
     CDS             9709..9993
                     /gene="rplW"
                     /locus_tag="PLX22_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT18868.1"
                     /translation="MNANDIIIRPVITEKATEMAKQNKYIFRVHKKANKDMIGRAINS
                     IFGVMPVKVNVMIVRGKRKRVRYNYGYTASWKKAIVTLKEGEKIDVFETK"
     gene            10027..10851
                     /gene="rplB"
                     /locus_tag="PLX22_02820"
     CDS             10027..10851
                     /gene="rplB"
                     /locus_tag="PLX22_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT18869.1"
                     /translation="MGLKKYRPITPTLRYQTVLDYSEITTDEPLKSLTKGKKGYAGRG
                     FKGQISVRRKGGGHKRRFRLIDFKRNKYNIEGKVSTVEYDPNRSANIALIIYKDGEKR
                     YIVAPFSLKVGDTVVSGDNVEVKPGNAMPLKNIPDGTSIYNIELHKGKGGQLVRSAGV
                     SAVITAKEGEYCLIKLPSGEIRKIYCECYATIGEVSNKDHINIDIGKAGRNRWMGKRP
                     KVRGVAMNPIDHPLGGGEGKSSGGRHPCSPTGKPTKGYKTRKKKKYSDKMIIKRRK"
     gene            10855..11142
                     /gene="rpsS"
                     /locus_tag="PLX22_02825"
     CDS             10855..11142
                     /gene="rpsS"
                     /locus_tag="PLX22_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT18870.1"
                     /translation="MARSLKKGPYVDIKLFKKIEEMNSTNAKKAIKTWSRRSTIFPEM
                     IGHTIMVHNGKNFIPVYVTENMVGHKLGEFAPTRTFRGHAGKDDKVAKRGK"
     gene            11143..11481
                     /gene="rplV"
                     /locus_tag="PLX22_02830"
     CDS             11143..11481
                     /gene="rplV"
                     /locus_tag="PLX22_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT18871.1"
                     /translation="METKAVSRYNRISAVKARLVANEIRGFSFPEAIDILRAIPRKAS
                     KLLMKTLYSAAAGAKYRNPEIRENNLYIKKIVIDEGPTMKRFRPRARGRASRIRKRLS
                     TIEIVLSDEN"
     gene            11502..12182
                     /gene="rpsC"
                     /locus_tag="PLX22_02835"
     CDS             11502..12182
                     /gene="rpsC"
                     /locus_tag="PLX22_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT18872.1"
                     /translation="MGQKVNPTGVRVGIIRTWDSVWFSEKDEYRKNLHEDIQVRRYIM
                     KKFKNAGIAKVGIERFPDRVNVYIHTARPGLLIGRKGVDIENLKVDLQKKINKNVNIY
                     VQEVKKPEKISKLVAEQVASQIENRFPYRRAIKQAITAAMRSGALGIKVSISGRLNGA
                     EMARYESYKEGRIPLHTLRADIDYGFAEALTTFGLIGIKVWIYSGDVLSNAGEQEEDK
                     FALRKQSR"
     gene            12202..12618
                     /gene="rplP"
                     /locus_tag="PLX22_02840"
     CDS             12202..12618
                     /gene="rplP"
                     /locus_tag="PLX22_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOT18873.1"
                     /translation="MLMPNRTRYRKVHRGRMKGKATRGSTIHFGEFALKTIECGEINS
                     RQIEAARIAISRKVKRGGKLWIRIFPDRPFTKKPAETRMGKGKGNPEGFVARIKPGRI
                     LFEISGIDDAMAREALSLAAHKLPVKTKIISIHDNN"
     gene            12634..12843
                     /gene="rpmC"
                     /locus_tag="PLX22_02845"
     CDS             12634..12843
                     /gene="rpmC"
                     /locus_tag="PLX22_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT18874.1"
                     /translation="MKGKLDELTVEELNRSLREAKEELRKERFKRTVSRVENPKKTMQ
                     LKKQIARILTVLREYESGTRTARSK"
     gene            12861..13115
                     /gene="rpsQ"
                     /locus_tag="PLX22_02850"
     CDS             12861..13115
                     /gene="rpsQ"
                     /locus_tag="PLX22_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOT18875.1"
                     /translation="MQENSRKQIVGKVISNKMNKTVVVEIERMMMHPKYHKYVRRTKK
                     VKSHDEKNECSIGDIVLIEETRPLSKEKRYRLVKIVEKVK"
     gene            13126..13518
                     /gene="rplN"
                     /locus_tag="PLX22_02855"
     CDS             13126..13518
                     /gene="rplN"
                     /locus_tag="PLX22_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOT18876.1"
                     /translation="MIQVQTMLEVADNSGVKRVQCIKILGGTRRRYASVGDVIIVAVK
                     DAHPSYGLKDSTGKKVHTKAVLRAVVVRTTKPVRRSDGSYIRFDDNAVAIIDNKGEPR
                     GSRIFGPVARELRDRNFMKIISLAPEVL"
     gene            13531..13848
                     /gene="rplX"
                     /locus_tag="PLX22_02860"
     CDS             13531..13848
                     /gene="rplX"
                     /locus_tag="PLX22_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOT18877.1"
                     /translation="MIRTILKKNDMVMVITGADKGKRGKILFVDRKRGRVVVEGVNVR
                     KKFVRPSQDNPKGGQIELPYPINISNVMVFCDKCKKAVQLGMKIDGDTKYRICKKCGK
                     RID"
     gene            13860..14408
                     /gene="rplE"
                     /locus_tag="PLX22_02865"
     CDS             13860..14408
                     /gene="rplE"
                     /locus_tag="PLX22_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT18878.1"
                     /translation="MAARLRMAYEKDVKKRMMDRFKYSSVMQVPKIEKIVLNVGEGEA
                     HSNPNLLQSIINELSLITGQKAVKTVARKSIAAFKIREGYDVGARVTLRGDRMYEFLD
                     RLINVALPRVRDFRGLSPNSFDNAGNYNFSVKEQIIFPELNLDKVEKIYGINITIKTT
                     ARNANEARALLEEFRFPFQEAR"
     gene            14415..14600
                     /locus_tag="PLX22_02870"
     CDS             14415..14600
                     /locus_tag="PLX22_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOT18879.1"
                     /translation="MASERMFAKARREPKFKIRYRNRCKICGRSRGYYRRFELCRICL
                     RKLAGEGLVPGVKKSSW"
     gene            14624..15025
                     /gene="rpsH"
                     /locus_tag="PLX22_02875"
     CDS             14624..15025
                     /gene="rpsH"
                     /locus_tag="PLX22_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT18880.1"
                     /translation="MSCVSDPIADMLTRMRNAINAGHVKVDIPSSKLKMAIAKILKDE
                     GFIKNFKLIDDNKQKLLRIYLKYSADNQSAIIRLKRISSPGRRVYLKAENLHPVYNNM
                     GIIILSTSKGIITNKAAKQLNIGGEVLCEIL"
     gene            15039..15584
                     /gene="rplF"
                     /locus_tag="PLX22_02880"
     CDS             15039..15584
                     /gene="rplF"
                     /locus_tag="PLX22_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT18881.1"
                     /translation="MSRIGKKPIVIPNGVSIDMQNNNLTVKGPLGVLQYQLQDVINIA
                     IENNTIAVSKKDEKSNDKKVNALWGLTRALINNMVIGVSSGFEKNLEIVGVGYRAIQK
                     NNDVEFQLGYSHTILFTPPPGTKVEVVEPTKIRVKGIDKQLVGQVSANIRKLRPPEPY
                     KGKGIRYKDEQILRKAGKTGK"
     gene            15619..15984
                     /gene="rplR"
                     /locus_tag="PLX22_02885"
     CDS             15619..15984
                     /gene="rplR"
                     /locus_tag="PLX22_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT18882.1"
                     /translation="MNRLKLKKVRYMRRRMRVKAKVRSNMQRPRMCISRSNKNLFVQI
                     IDDGQGSTLVCASTLEKDFPVVKNRGNKEAAKALGTIIAQRAIAKNIKEVVFDRNGYL
                     YHGRIKEFADAARENGLEF"
     gene            15996..16511
                     /gene="rpsE"
                     /locus_tag="PLX22_02890"
     CDS             15996..16511
                     /gene="rpsE"
                     /locus_tag="PLX22_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT18883.1"
                     /translation="MKITRKRIKPEGLELAEKIIIINRVAKVVKGGRRFSFNAMSVVG
                     DNHGHVGIGFGKANEVPDAIRKSVEDAKKNVYKINVNKNTIPHEVIGRFKSTRVVLKP
                     AAPGTGIIAGDSVKAVVEKAGIVDIMSKTLGSRNPVNVTKATINGLLQLRSLKEVSDL
                     REIILPKLWQS"
     gene            16523..16711
                     /gene="rpmD"
                     /locus_tag="PLX22_02895"
     CDS             16523..16711
                     /gene="rpmD"
                     /locus_tag="PLX22_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOT18884.1"
                     /translation="MAKKLLIKQTRSYISAKPKQKATLRALGLRKLNAERVHDNTAVI
                     RGMVDKVKHMVKVSEINE"
     gene            16729..17166
                     /gene="rplO"
                     /locus_tag="PLX22_02900"
     CDS             16729..17166
                     /gene="rplO"
                     /locus_tag="PLX22_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOT18885.1"
                     /translation="MQSTIKKPEYIKKSKRVGCGQGSGHGKTSCKGHKGQKARSGAHF
                     KPGFEGGQMPLQRRIPKRGFNNTKFKEEVQVVNINDITKLSVKEITPEVLYKEGLIKS
                     QFLPVKLLAVGDINDAIAVVVDAISTTAKDKIIKAGGSVTLRA"
     gene            17194..18540
                     /gene="secY"
                     /locus_tag="PLX22_02905"
     CDS             17194..18540
                     /gene="secY"
                     /locus_tag="PLX22_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOT18886.1"
                     /translation="MPNVISNIFKIPELRKRVLFTLFILVVYRIGAHIPIPGVNIEAL
                     SSYFEMAAKSRGSGFVDFFDLFAGGALKRFTIFALGIMPYISASIIMQLLTVVIPAIE
                     KLSKEGPEGYKKINQYTRYGTVLLCALQGFGLTMWMKSMTSPKGAVVPQDSGIGFVLV
                     AVLAITTGTMILMWLGEMITERGIGNGISLIIYAGIVVRAPSAFVDTMNRIISRSDPI
                     IALILIALFIGVIAASILLTLGVRKIPVQYGKRVVGRRMVQMASQAIPLRVNAAGVIP
                     IIFASSIILFPAQISNMLGATKYGIFTKMQYWLSPGHTAYMIIYSLLIIFFCYFYTAI
                     QFNPNDLAENLKKYGGFIPGIRPGQNTADYIMHILNRITLPGSIFLALIAIFPDFIIR
                     IWPEEVSPEMAYLFGGTSLLIIVGVALDTLKQIESQLLMRHYDGFFKKGRIKGRRY"
     gene            18588..19340
                     /gene="map"
                     /locus_tag="PLX22_02910"
     CDS             18588..19340
                     /gene="map"
                     /locus_tag="PLX22_02910"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT18887.1"
                     /translation="MAIYIYNEDEIKRVEDACRITAAVLEELGKNVVEGISTFELDRV
                     ARDLIRKSNAIPAFLGYKGYPAAICTSVNEVVVHGIPHRNQILRKGDIVGIDIGVKFK
                     DFFGDAAKTFIVGKVNNKVKKLLEVTKEALELGILQCKDGNHLTDISHVIESHVVQWG
                     FQPVHEFVGHGIGRNMHEEPSIPNFGKPGKGPIIKTGMIFAIEPMINEGTYQVEILDD
                     GWTTITKDRKNSAHFEHTVAVVHGSPKILTMV"
     gene            19367..19585
                     /gene="infA"
                     /locus_tag="PLX22_02915"
     CDS             19367..19585
                     /gene="infA"
                     /locus_tag="PLX22_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT18888.1"
                     /translation="MAKEEPIEVEGEVVEALPNAMFRVKLDNGHVVLAHISGKMRMHY
                     IRILPGDKVTVELSPYDLSRGRITYRAK"
     gene            19609..19737
                     /gene="rpmJ"
                     /locus_tag="PLX22_02920"
     CDS             19609..19737
                     /gene="rpmJ"
                     /locus_tag="PLX22_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT18889.1"
                     /translation="MKVRVSVKKICSDCKVIKRHGVVRVICKNPRHKQRQKKRTAK"
     gene            19756..20130
                     /gene="rpsM"
                     /locus_tag="PLX22_02925"
     CDS             19756..20130
                     /gene="rpsM"
                     /locus_tag="PLX22_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT18890.1"
                     /translation="MARIAGVDLPNNKRVEIALTYIYGIGLPSSLKILEEARVNPDTR
                     VKDLTDEEINQLRKIIEKSYKVEGDLRTQIAMDIKRLMDIGCYRGIRHRRGLPVRGQN
                     TKNNAHTRKGNRKVSVGGKKKK"
     gene            20154..20540
                     /gene="rpsK"
                     /locus_tag="PLX22_02930"
     CDS             20154..20540
                     /gene="rpsK"
                     /locus_tag="PLX22_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT18891.1"
                     /translation="MKQKRTKKQEKKNIPVGKAYIQATYNNTIITITDMQGNVVSWAT
                     SGGEGFRGSRKSTPFAAQMAAKAAAQKAIDNANMRSIEVYVKGPGIGREAAIRSLYQA
                     GLNISKIKDITPIPHNGCRPRKRRRV"
     gene            20557..21186
                     /gene="rpsD"
                     /locus_tag="PLX22_02935"
     CDS             20557..21186
                     /gene="rpsD"
                     /locus_tag="PLX22_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT18892.1"
                     /translation="MAKYTGPLCKLCRREMTPLMLKGRRCLTDKCAIKHKKYPPGPPK
                     KRRIKLSDYGVQLREKQKIKRSYMLLETQFKRVFEEATRLKGVTGENMLVLLERRLDN
                     VVYRMGFASSRLQSRQLVQHGHVIINGKRVDIPSYQVKVNDIVEIKEDFKNNAILEDA
                     IKLSKAVGSVPQWVEVDYEKRNGKIIRLPERKDIEPIFNEQAVVELYSK"
     gene            21227..>21620
                     /locus_tag="PLX22_02940"
     CDS             21227..>21620
                     /locus_tag="PLX22_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT18893.1"
                     /translation="MSYKNLLKGFRRPSKIVFEHDEVNQDYGRFIAEPFERGYGLTIG
                     NSLRRVLLSSIEGAAITAVKIQGVPHEFMTIDGVVEDVTRILLNLKRLRFRYASELPK
                     VLHIVKDGEAELTGADFSVDSDVEVMNPD"
BASE COUNT         7442 a         3013 c         4995 g         6170 t
ORIGIN      
        1 ctttttaaac catttattat gaagcgtttg gttgataaag gttttgtcca gaatataaaa
       61 tcagccaaaa aaatggttga aaatgaaaga cctgaagttt gggaggttct ggaagaggtt
      121 atttctgaac accccgtatt gttgaaccgt gctcccacat tgcatagatt ggggatacaa
      181 gcatttgaac caatactggt ggaaggaaag gcaattaaat tacatccact tgtatgtcat
      241 gcttataatg ctgattttga tggtgaccag atggcagttc atgtgccgct atctccgcgt
      301 gcccagatag aatgctggat attgatgctt tctgcaaata atctactcaa ccctgcaaat
      361 ggacagccta tagtaacgcc tacgcaagat atagtattgg gtgtttatta tctaacatct
      421 gaattgaaaa gtaaatctga acatttacca tattttggtt caatggatga aatattgtat
      481 gctctggaat ataagcagat taaatatcgt tccaggatta agtatttgaa ggatggagag
      541 tttattgaaa caactccagg gaggttaatc ttcaatgaaa aacttcctgc tggttttcca
      601 tttgtcaact atactattaa tgataaggaa ctatcaaaaa taatttcaaa gatattcaga
      661 gggtatggtc cgacagctac tgttaaggtg ctcgatgatg tcaaagagct gggatacagg
      721 tttgctacct attttggccc tactatctct accagtgatg ttaaagtgcc tgaaatgaaa
      781 gatgaattaa tcaaaaaagc taatgagatg ttagaaaaaa ttgaaaatga atatcgtgat
      841 ggatggcgca ctaatgagga gcgatacaac agagttatag acttgtggac aaaaacaaat
      901 gaagatgtta cagaggtgat gttcgatacg atggcacagg atgccgatgg ttttaatcca
      961 atttacatca tggcacaatc aggtgcaaga ggcagtaaac agcaaatacg acagttagct
     1021 ggcatgcgag gcttaatggc aaaacccagc ggtgagatta ttgacgtgcc cattaaatca
     1081 aactttaagg aagggttgac tgttcttgaa tattttattt caaccaatgg tgcacgaaag
     1141 ggattggcag atactgcttt aaagactgcg gatgcagggt atcttacccg cagactggtt
     1201 gatatagctc aggatgttgt tataaccatg gatgattgtg gcacatcagt tggtattgat
     1261 gttactgcga taaaagaagg tgatgaggtg atcgaatcaa ttggttcaag agctctgggc
     1321 agaacattgc tctatgattt atatcatcct gttaccaatg agagtatttg taatagcggg
     1381 gagcttatta ctgaggatat agcagaggag atagatgagt tagggcttga tcgtattacc
     1441 atacgttctg cgctgacatg cgaagctcgt catggggtat gtgcacgatg ctatggaaga
     1501 aatttagcaa cttcacgaat ggtggatatt ggtgaggctg ttggtatcat tgctgcacaa
     1561 tcaattggcc agcctgggac acagcttacc atgcgtacct tccatattgg tggtactgct
     1621 tcgcattttg tggaggaaag tgaaattagt tttaattatc cggtgtatgt aaaagaaaca
     1681 acctttaaaa ctgttaaaac tgaagatgaa aaaggtgaaa agaagactat ttctgttaga
     1741 cgtggataca ttataataca acggttgtta gaagaaatct ctgcaaaaaa cggtgatatt
     1801 ttggttaata atggagatag agtttatagt gggacaccag tagtcaagaa acccgacggg
     1861 tcctttatta ctacctccaa agcaggtatt gtaaagttgg ataagtcaag aatatatatt
     1921 ctgggcgaac aaaatatgat cccggttaat attggatcaa ctatttattg taaaactgat
     1981 aaaatatatc ctgtagatcc tcaaacaggg aaaacagaga tgctggttgg ttttgatccg
     2041 tggtttgaac cgatcctgac tgaaatcaat gggaaagtca aatttattga tatagaaatt
     2101 aacaagagta tgcgcgagga gatagatcct ttaactcacc agctcaaacg aatcatagtt
     2161 gaaacaaaaa ccgagaaatt acagccggca attgaagtat cttctgctga ggaattattc
     2221 cattatcttt tgcctaaagg tgcacatttg tttgttgatg atggcatgtc agtaaaagcc
     2281 ggaactgtgt tggcaaagat tccaaaagag gttgaaaaaa caaaagatat tactggtgga
     2341 ttgccacggg ttgctgaatt gtttgaagct agaacaccaa aagaaaaagc aacaatatca
     2401 gaaattgatg ggtatgtcaa gattggcgat acagtaaaga ataaacgtaa gattattatt
     2461 actgctgaag atggagactt tagagaatat cagataccgg catcaaaata tttacgagta
     2521 caggatgctg attggatcaa taaaggtgat aagattgatg atggaccaat tgatccacat
     2581 gatatccttg acataaaagg accccgcgaa ttggagcaat ttcttgtaaa tgaaatacag
     2641 gaggtttaca gattgcaggg tgttcagatt aatgataagc atattgagat aatagtacgg
     2701 cagatgttgc gaaaagttat tattgaagat ccaggggata caagctttgt tgttgatcag
     2761 attgttgata aatttgagtt tgacgatgaa aatatccgtg ttgaaaagga aggtggcaag
     2821 gctgcatcag caaagccagt gcttatgggc ataacaaagt cagctcttaa taccgagtca
     2881 tttatttcag cagcatcatt ccaggaaaca accagagttt taacggaagc agcaattaaa
     2941 ggtaagattg ataggctgcg aggtcttaag gaaaatgtta ttattgggca tttaattcct
     3001 gccggcacag gagtacgtga ttataacaag gtaaccgttt atcagaaaat acctggtgat
     3061 ttagatggcg ttgtgaaaga aatcgaggaa gaaatttctg tagagaagta aattttcttg
     3121 acaattaaaa gggtaaaatt gattaatcac attcagttaa aattttgaaa cagaaaaatt
     3181 ataatattat agtgaacatt aataaatgat gataaggaga tggaatgcct acaattaatc
     3241 aattaataag gaaagggaga tcagaaaaag taaaaaaatc aaaatctcca gcattaaaaa
     3301 gctgtcccca aaagcgtggt gtttgtacca gggtgatgac cgtaacacct aaaaaaccaa
     3361 attcagcgtt acgaaaggtt gcacgtgttc gacttactaa tgggatagag gtaacagcat
     3421 acattccggg tataggtcac aatttacagg aacactcggc agttttagtc cgtggtggaa
     3481 gggtgaagga tttacccggt gtcaggtatc atatagtacg tggtacattg gattcattag
     3541 gtgttaatga caggaagaag ggtcgttcca aatatggcac aaagaggcct aaagcataaa
     3601 tgatatcgaa acgttgttac tttgatggag aagtaatgtt tagagatgtc catacaagtg
     3661 atttgaaaat ttgtaatagc gaggtttaag aatggcacga aggcgtagac cagcaaaaag
     3721 aactatctta ccggatccca aatatggaaa tatagttgtt tcaaagttta tcaactctat
     3781 gatgacagaa gggaagaaag gtgttgctga aagagcattt tataaagccc ttgaattgat
     3841 aggacaacgc acccagaaag atcctattga agtatttata aattcactgg aaaatgttaa
     3901 gccgcttgta gaggttaaat cacgaagagt ggggggggct acgtatcagg taccagttga
     3961 aatacgtcct gaaagaagac aggcattagc tattcgatgg attgttactc attcacgcaa
     4021 ccggagcgag aaaaccatgt tccaaaaatt agccggtgag ctattagatg cttttaataa
     4081 tactggtaca tcaatcaaga aaaaagaaga tactcataaa atggcagaag caaataaagc
     4141 gtttgcacat tataaatggt gatatattta cttattcagg cttttatagt ataataataa
     4201 aaaaatacca ggggtagccc cctggtattt ggtgctttag cttaaaaata aatatttaaa
     4261 atacattgat gattaagtgt taattatggg aagagaactg ccaatacaca ggaccagaaa
     4321 tattggcatc atggcgcata ttgatgccgg gaaaactaca ttaactgaac gtatacttta
     4381 ctataccggt aaaacctata agattggtga agtacatgac gggactgctg aaatggactg
     4441 gatgatacag gaaaaagaac gtggcattac tataacagca gcagctacca caacctactg
     4501 gaaaaataca cgaattaata ttattgatac tccaggtcat gtagatttta ctgtggaggt
     4561 tgagcgcagt ttgcgagttt tagatggggc tattgctgta tttgatgcag tagctggtgt
     4621 tgaaccgcaa agcgaaacgg tatggcggca ggctgaccat tataatgttc cacgtatatg
     4681 ttttattaat aaaatggaca gggtaggagc tgattttgaa cgctgtctta ccatgataaa
     4741 agaacgattg tattccattc cccttccatt gcagataccc tatgggaaag aagatatttt
     4801 taggggtgtt attgatctga tacaaatgaa aggaataata tggactgatg catcaggtga
     4861 gcactatgaa tataccgcta tcccggatga attgcaaagt gaagcccaat actatcgtga
     4921 gcacctcatt gaaacagtag cggaacatga tgagcagatt cttgataaat atctggcagg
     4981 agttgaaata gatgaacaag agttaaaaag agctatccgc actgttacaa taaaatcagc
     5041 attatttcct gttttttgtg ggagtgcatt aaagaataag ggagtgcaac cattgattga
     5101 tgctattgtg gattacttac cctcgcccaa agacatccct tcaattaaag ggaatgatgc
     5161 ccaccatgat gatgttatag tggaacgcca tcccgatgat aaggaaccat tttgtgctct
     5221 ttgttttaaa atcatgactg atgcgtatgt gggaaaatta tgctatgtac gtgtttattc
     5281 cgggcatgta tcggtaggtg aatccattct aaatgtcaac aaacaaaaaa aggaaagaat
     5341 acaacgcata ttacgaatgc atgcaaatga cagagaagat attaaggagc tatatactgg
     5401 tgatatagcg gcgattgttg gttttaaatt tgctcaaact ggtgatacta ttacttcaat
     5461 tgacgcaccg atagtattag gaaaaatgga attccccgaa ccggtgattt ctatagccat
     5521 tgaacccaaa acaagagctg atctagaaag gttgcatatg atattaaaac gccttgttga
     5581 tgaggaccct accatttttg ttaaagttga tgaagaaagt gggcagactg ttctttcagg
     5641 aatgggtgag cttcacctag agattatagt tgatagattg caaagggaat ttaatcttcc
     5701 agttaatata gggaaaccac aggttgccta taaagagaca gtttctacaa ctgcaaaagc
     5761 tgagggtatt tttgaaaagg aaattggagg taaattacag tatggacacg ttttgctgga
     5821 ggttagtcca tgcgatagtc actttgaatt tatatcaaaa ttagatagtg cggctatacc
     5881 agatataatt attaaagcaa tcgaaatggg atgcagggat gcgcttaatt ccggtgttat
     5941 tgcaggatat gaaatgacca gaatacgggt gatagtggaa gatgctaaat accgtgaaga
     6001 tgtatcagta gagcatgcat acaggattgc tacgacaatg gcgtttagag atgctgtaaa
     6061 aaaggcaact cctgtgttgt tagagccaat tatgaaactg gaggcggtag ttcctgacga
     6121 atatatgggt gatattatca atgatatcaa tgcacgaagg gggaagatag aacatatcga
     6181 catacgaggt atgttgaaag ttattgatgc ttatgtaccg ctatcagagg catttgggta
     6241 tgctacggca gtacgttcgt tgagtcaggg tagggcatca cacacactgc agtttaacca
     6301 ttatgatgta gtgccacaaa atgttatgga gaaaataata gggagttact atagacctgt
     6361 ttaatgaatc tgatattata aatggtatta attaattttt atttttttta aaaaatattt
     6421 gatattttta aaattacgca ataaattagt tgacagatat agcatatatt ttttaattca
     6481 cacacttcca ccttttgtcc ctcaatagat gatgtgggag attgtgttat atagagtgaa
     6541 tacattcttg taattgaaga ttgttaaaat tttattatat ctatagtaaa gtagagtatg
     6601 cttaatctgt ttttttttgc taagttgaaa attattattt tgatgaagga gataacaaat
     6661 ggcaaaggca aaatttgaac gaacaaaacc gcatgtcaat gttggtacta ttggtcatat
     6721 cgatcatggt aaaacaacat taacctctgc aattacaaag gttttgacag cagcgtatgg
     6781 cggtcataat aaacctattg attttgatca gatagacaat gcacctgaag aaaaagcaag
     6841 aggtataacg attgccacgt cgcatcagga atatgaaact cctaataggc attatgcgca
     6901 tgttgactgc cctggccatg ctgactatat caaaaacatg atcaccggtg ctgcccagat
     6961 ggatgttgca atattagttg ttgctgcgac ggacggccca atgccacaga caaaagaaca
     7021 tgtcttattg gcacgacagg taaatgttcc ttatattatt gttttcttga ataaagttga
     7081 catgcttgat ccggctgata gagacgagct tgttgaattg gttgaaatgg agatccgtga
     7141 tttactgaat aaatatgagt ttcctggcga tactatcccc attattcctg gttctgcact
     7201 aaaggcaatg cagtgtggtt gtgggaaagc ggattgtgag gcatgcgggc caatacttaa
     7261 attagctgag acgcttgaca cttatgtgcc tgagccaaaa cgtgacaagg ataaaccgtt
     7321 cttgatgcct attgaggatg tattctctat tacaggacgc ggtacggttg taacaggaag
     7381 aattgagcgt ggaatggtga gaactggtga tgaagttgaa attgtaggtt ttggcgaaac
     7441 aaaaaagtcg gttgttaccg gtgtagagat gttcagaaag atacttgacg aaggtatggc
     7501 tggtgacaat gtaggttgtt tgctacgcgg tattggcaaa gaagaggtgg agagaggaca
     7561 ggtattagca aagccaggtt ctataactcc ccatagaaaa tttgaagcag aagtatatgt
     7621 cctgtcaaaa gaagaaggtg gacgtcatac gccatttttt actaattacc gacctcagtt
     7681 ctatttcaga acaactgatg taactggtat aattcagctt cctgaaggtg ttgaaatggt
     7741 tatgccgggt gacaatatta agatgactgt tgagcttatt actcctgttg ctatggaaga
     7801 aacacttcgt tttgcaattc gagaaggtgg tagaactgtt ggtgcaggtg tagttgccaa
     7861 gatattagaa taagactgtt ctatttagta tgatagcaat ggtagtagtt gtcaggctgg
     7921 tatatatcaa cctgacaatt gtttagaata gtatgcaaaa tgataattgg tggtgggatt
     7981 gtagggatac aatcagggaa ctggatgatt actactatat taatttatga ggatataata
     8041 aggatattac agtggctaag cttgcgggac aacgtataag agtgaaatta cgatcatttg
     8101 atccatattt attagatcaa tcagcagcta agatagttgc gacaactaac aggagtggtg
     8161 caaaggttgc agggcctata ccgttaccta cacgtattga aaaattttgt gttttgcggt
     8221 ctcctcatgt taataagaag tcgcgtgaac agtttgaaat ccgaactcac aaacgcttaa
     8281 ttgatattat tgatccaaat tccgacactg ttgaagcgct tatgaaatta gaattgcctg
     8341 ctggtgtttc tgtagacata aaatcataaa ttcaacattg atatgatgtt agttaaagag
     8401 ctcttttaga agagtgtggt tgtcttataa ttgatgtatg ggatgggaaa atgaaaaagg
     8461 cgttaattgg tagaaaaatt gggatgagtc agttgttttt agatgatggg acggtaatcc
     8521 ctgttactgt atgtgaactt ggtccttgcg ttgttgttca gaaaaagacc attgaaaaag
     8581 atggatacaa tgctttaaaa ttggggtatt gggaaacaaa acctcaccgt cttactaaac
     8641 ctataataga ggatttgaag aagaagaatc tcccaattgt taaagtgttt aaagaagttg
     8701 atatttttga tgaagattta attgaaggaa gtataataac ctgtgatgtc ttttctgaga
     8761 accagatagt ggatgtatcc ggtacatcaa aaggtagggg ttttgctggg gttattaaaa
     8821 ggtacgggtt ccatggcgga aaagaaacgc atggttcaaa tttccataga gcacccggat
     8881 caattggtgc gaatacatac ccggctgagg tgtggaaggg gaaaaagctt ccgggcagac
     8941 acggtggaaa aactgtgacc gttaaaaacc ttaaaattat aaggataatg aaagataaga
     9001 atttggttat tatttcaggt gctatcccgg gtaccgtcaa ttctattatt acagtacaga
     9061 gtaaataggc aggattgtta tggttgtaga tgtttataca cttgaaggaa acataaaagg
     9121 gcagattgag ctgaatgaat caatctttaa tgcaaaggtt aatgatgttt tgatgtatga
     9181 attgattaaa gcagctaatg ccaatttgcg gcaggggaca cacgatacaa aagagcgatc
     9241 atatgtatcg ggcggtggag caaaaccatg gcggcaaaag gggacgggaa gagcacgtca
     9301 gggtagtatc cgtgctccac agtggaaagg tggtggtgcg gtgtttggac ctcatccaag
     9361 ggactatcgc atagaattgc caaaaagaat taaacatgaa gcatatcgtt ctcttttatc
     9421 aatgaaattt aaagagggtt ctataaagat aattgaggat tttaaagttg tagatggtaa
     9481 aacaaaagaa gttgctgcca ttgctaaaaa gctcaatatt agcaaaggag tacttattac
     9541 tgatattgaa gacaatttat tgaaacgtgc aatacgaaat ataccatggt ttttatataa
     9601 taatgttaat cgtttgtctg gcagggatat tttttataca aagactgtgt taattaccga
     9661 aaacgcagca aagattatta attcgaaata tgcaaaaggt gaataattat gaatgccaat
     9721 gatattatta tacgcccggt gattacagaa aaagcaacgg aaatggcaaa gcagaataag
     9781 tatatattca gagttcataa aaaagctaat aaggatatga tagggcgtgc aataaacagt
     9841 atctttggcg ttatgcctgt aaaggttaat gtaatgatcg taagaggtaa gcgtaagcgt
     9901 gtacgttata attatgggta tacggcttca tggaaaaaag cgatcgttac attaaaagaa
     9961 ggcgagaaga ttgatgtttt tgaaacgaaa taaggaatta tcagtatagc gataaaggta
    10021 acgaatatgg gtttgaaaaa atatagacca ataacaccga cattacgata tcaaacggtt
    10081 ttagattaca gtgagattac tactgatgaa ccattgaagt cattaactaa aggtaagaaa
    10141 ggttatgctg gtagaggctt caaaggacag atctccgttc gcagaaaagg aggtgggcat
    10201 aaacggcggt tccgactcat tgattttaaa cgtaataagt ataatattga aggtaaggtg
    10261 tctactgtcg aatatgatcc taatagaagt gctaatattg ccttgataat ctacaaagat
    10321 ggggagaagc gatatattgt tgcacctttt tctttgaaag taggggatac agtagtttca
    10381 ggggataatg ttgaagtaaa acctggaaat gctatgccct taaaaaatat accagacggt
    10441 acgtctatct ataatataga attacataaa ggcaaaggtg ggcagttggt gcgttctgct
    10501 ggagtaagtg cagtcattac cgcaaaagaa ggggaatatt gcttgataaa actaccatca
    10561 ggtgaaatca gaaaaattta ctgtgagtgt tatgctacaa ttggtgaagt gagcaataaa
    10621 gaccatatta acattgatat tggtaaggct ggtcgcaaca gatggatggg taaaagacct
    10681 aaggtacgtg gtgttgcaat gaatcctatt gatcatcctc ttggaggtgg cgaaggaaaa
    10741 tcatcgggag ggcgacatcc ctgttctcct actggcaagc ctacgaaagg atataaaaca
    10801 cgaaagaaga aaaaatatag tgataaaatg attattaaac gaaggaagta agctatggct
    10861 cgttcattga aaaaaggacc gtatgttgac ataaagctgt ttaaaaaaat tgaagaaatg
    10921 aactcaacca atgcaaagaa ggcaattaaa acatggtcac gaagatctac catatttccg
    10981 gagatgattg gacataccat tatggtacac aatgggaaaa attttatacc ggtgtacgtt
    11041 actgagaata tggtagggca taaattgggt gagtttgcac caacaaggac atttagaggc
    11101 catgctggca aagatgacaa ggtagcgaaa agaggcaagt aaatggaaac gaaagcagta
    11161 tcacgatata acaggatctc tgcggtgaaa gcacggttag ttgccaatga aataagaggt
    11221 ttttcatttc ctgaggctat tgatattctg agggcaatac cgcgtaaagc atcaaagctt
    11281 ttaatgaaaa cactctattc agcagctgca ggtgcaaagt atcgcaatcc tgagatacgt
    11341 gaaaataatt tatatatcaa gaagattgta attgatgaag gtccaacaat gaagcggttc
    11401 aggccaaggg ctcgtgggag ggcttcgcgc atacgcaaac gattgagtac aattgagata
    11461 gtcttatctg atgaaaatta atgtaagttt aaggtgtaat aatgggacag aaagttaatc
    11521 caacaggtgt aagagtaggc ataatcagaa catgggattc tgtttggttt agtgaaaagg
    11581 atgaatatcg taagaatctc catgaagata ttcaggttag acgatatata atgaaaaagt
    11641 ttaaaaatgc agggattgca aaagttggta ttgagcgatt tcctgataga gtcaatgttt
    11701 atattcatac agcaaggcca gggttgttaa ttggaagaaa aggtgtagat attgaaaatc
    11761 tgaaagttga tttacaaaag aagattaaca aaaatgttaa tatatatgta caggaagtta
    11821 aaaagccaga aaaaatttct aaattggtag cagaacaggt tgcttcacaa attgaaaata
    11881 gatttcctta tagaagagct atcaagcagg ctattactgc agccatgcga agtggggcat
    11941 taggtatcaa ggttagtata tccggacggt tgaatggggc tgaaatggcc cgatatgaat
    12001 cgtataaaga aggaagaata ccattgcata cactgcgtgc tgatattgat tatggatttg
    12061 ctgaagcatt aacaacattt ggtctgattg gcattaaggt atggatttac agtggtgatg
    12121 ttctctcaaa tgctggagag caagaagaag ataaatttgc attacgaaaa caatcacgat
    12181 aattgatgaa ggtataatat tatgttaatg cctaatagaa ccaggtatag aaaagttcac
    12241 cgtggacgta tgaaaggaaa agcaacacgt ggttcaacaa tacattttgg tgaatttgct
    12301 ctaaaaacga ttgaatgcgg tgaaattaat tcccgacaga ttgaagctgc ccgtattgca
    12361 attagtcgta aagtaaaacg tggcggtaaa ttatggataa ggattttccc tgacagacca
    12421 tttaccaaaa aaccggctga aactcgtatg ggtaaaggta agggaaatcc tgaaggtttt
    12481 gttgcgcgga ttaaacctgg aagaatattg tttgaaattt ctggtattga tgatgccatg
    12541 gcgcgtgaag cattgtcatt agctgcacat aaattaccag tcaaaaccaa aataatatca
    12601 atacatgata acaattagta ttggagttga tcaatgaaag gtaaactgga tgaactaacg
    12661 gtagaagagc ttaatagaag tttgcgtgaa gcaaaagagg aattaaggaa agaacgtttt
    12721 aagagaacag ttagcagggt tgaaaatcca aaaaaaacca tgcaactgaa gaagcagata
    12781 gcaagaatat taacggtgct acgtgaatat gaatcaggca caagaacagc aagaagtaaa
    12841 taattttggt ggtaacagct atgcaagaaa acagcagaaa acagattgtt ggtaaagtta
    12901 taagcaataa aatgaacaag acggtagttg ttgaaattga gagaatgatg atgcatccta
    12961 aatatcataa gtatgtgcga agaaccaaaa aggtcaaaag ccatgatgaa aagaatgaat
    13021 gttcaattgg tgatatagta cttatagaag aaaccaggcc gttatctaaa gagaaaagat
    13081 atcgtttggt gaagatagtt gaaaaggtaa aatagggtta gtgctatgat tcaggtacaa
    13141 acaatgttag aagttgcaga taatagcggt gtgaaacgag ttcagtgtat aaaaatttta
    13201 gggggaactc gaaggcgtta tgcttcggtt ggagatgtca ttattgttgc cgttaaagat
    13261 gcacatccat catatggatt gaaggattcg acagggaaaa aagtccatac aaaagctgtt
    13321 ttgagggcag ttgtagtaag gacaaccaag ccggtacggc gttctgatgg ttcatatata
    13381 cggtttgatg ataacgccgt tgcaattatt gataataaag gcgagcctcg aggttctcgt
    13441 atttttggtc ctgtagctcg agagttgcgg gatagaaatt ttatgaagat tatttcattg
    13501 gcaccagagg ttttataaag gattaagctt atgataagaa caatattaaa aaagaatgat
    13561 atggtaatgg ttattaccgg tgcagataaa ggtaaacgcg gtaaaatact ttttgtagac
    13621 agaaagcgag gaagagtggt agttgaaggt gttaatgtaa ggaagaaatt tgtacgtcct
    13681 tcacaggata atcctaaagg ggggcagata gaattgccat atccaattaa tatctctaat
    13741 gttatggttt tttgtgataa atgtaaaaaa gctgttcaat tgggtatgaa gatagatggt
    13801 gacaccaaat atcgaatatg taaaaaatgt ggcaaacgaa ttgattagtg agggtaatga
    13861 tggcagcacg attgcgcatg gcatacgaaa aggatgtaaa aaaaagaatg atggataggt
    13921 ttaaatattc atctgttatg caggtcccaa aaattgagaa gatagtatta aatgttggtg
    13981 aaggtgaagc tcattcaaat cctaatcttt tgcagtctat tattaatgaa ctatcactta
    14041 taaccgggca aaaggctgtt aaaacagttg cacgaaaatc aattgctgct ttcaagattc
    14101 gtgaaggtta tgatgttggt gctcgtgtta cattgcgagg cgatagaatg tatgaatttt
    14161 tagatcgact tatcaatgta gccttaccgc gagtcagaga cttcagagga ctatcgccaa
    14221 actcttttga taatgcgggg aattataatt tttctgttaa agaacagata atattcccag
    14281 aattaaatct ggacaaagtt gagaagattt atgggattaa tattacaatt aaaaccactg
    14341 ccagaaatgc taatgaagcc agggcattac tggaagaatt cagatttcca ttccaagaag
    14401 cgaggtaagt tgaggtggct agcgaaagaa tgtttgcaaa ggctagaaga gagcctaagt
    14461 ttaaaattcg ttacaggaat cgatgcaaaa tttgtggtag gtctcgtggg tactatcgcc
    14521 gttttgaact ttgcaggatt tgcttgcgaa agttggcggg tgaaggtttg gtaccaggcg
    14581 ttaagaaatc gtcgtggtaa aatttaaaag aggtaaataa gatatgagtt gtgtttctga
    14641 tccaattgca gatatgttga cgagaatgcg gaatgcaatt aatgcaggac atgtgaaggt
    14701 tgatatacca tcatctaaat tgaaaatggc tatcgcaaag attttaaaag atgaaggatt
    14761 tattaaaaat tttaagttaa ttgatgataa taagcaaaaa cttttgcgta tttatctgaa
    14821 gtatagtgca gataatcagt cggcgattat tcggcttaaa cggataagct cgcctggaag
    14881 aagggtatat cttaaagctg agaatttaca tccggtttat aacaatatgg gcattataat
    14941 attatctaca tcaaaaggta ttattaccaa taaagccgcc aaacaactta atattggtgg
    15001 tgaagtttta tgcgagatat tataaggggc agcaggatat gtcaagaata gggaaaaaac
    15061 cgatagtgat accgaatggt gtatccattg atatgcagaa taataattta accgttaaag
    15121 gacctctggg ggtacttcaa tatcagttgc aggatgtgat aaatattgca attgaaaata
    15181 acactattgc tgtttcaaaa aaagatgaaa aatctaatga caaaaaagtt aatgcattgt
    15241 ggggcttaac cagggcatta attaataata tggtaattgg agtatcaagt ggttttgaaa
    15301 aaaatttaga gatagttggt gttggatata gggcaataca aaaaaataat gatgttgaat
    15361 ttcaattagg atattcacat acaattttgt ttactcctcc gccaggtaca aaggtagagg
    15421 tggtagaacc aacaaagata agagttaagg gtattgataa acaacttgta ggtcaggtat
    15481 ctgcaaatat tcgaaagctc cgcccgccag aaccttataa aggaaaaggc attagatata
    15541 aggatgaaca gatactgaga aaagctggta aaactggtaa ataatgctat gagaatgaga
    15601 atatgaagga tagttattat gaatagattg aaattaaaaa aagtcagata tatgcgcaga
    15661 aggatgcgtg ttaaagcaaa agtcagaagc aatatgcagc gtcctcgcat gtgtatttcc
    15721 cgaagcaata agaatttatt tgtgcagatt atcgatgatg gtcaaggaag tactcttgtt
    15781 tgcgcttcta cccttgaaaa agattttccg gttgtaaaaa atagagggaa taaagaggca
    15841 gcgaaagcac tgggtacaat aattgctcag cgagctattg caaaaaacat aaaagaggtt
    15901 gtatttgata gaaatggtta tctttatcat ggtaggataa aggagtttgc tgatgcagct
    15961 cgtgaaaatg gattagaatt ttaggggtga tatggatgaa gatcacaaga aaacgtataa
    16021 aaccggaagg attagaactt gctgaaaaaa taataataat caaccgagtt gcaaaggttg
    16081 taaaaggcgg tagacggttt tcatttaatg ctatgtcagt tgttggtgat aaccatgggc
    16141 atgtgggcat tggttttggg aaagctaatg aggtgcctga tgcaatccgt aaaagtgttg
    16201 aggatgctaa gaagaatgtt tataaaatta atgtgaataa aaataccatt cctcacgagg
    16261 taataggcag gtttaaatct acaagggttg ttttaaaacc ggctgcacct ggtactggca
    16321 taatagcggg tgattcggtt aaggcagtag tggagaaggc gggaattgtt gatattatgt
    16381 caaaaacatt gggttcacgt aatccggtta atgttacaaa agcaacaata aatgggttgt
    16441 tacaattacg tagcttaaaa gaagtatctg atttgcgtga aattatttta ccaaagctgt
    16501 ggcaatcata agaggtggat atatggctaa gaaattattg ataaagcaaa cacgtagtta
    16561 tataagcgca aaaccaaagc aaaaagctac attacgtgct cttggcttgc gtaagttaaa
    16621 tgctgaaaga gtacatgata atactgcagt tatacgtggt atggttgata aagtaaaaca
    16681 tatggtaaaa gtttcagaaa taaatgaata actatgggta taaatgatat gcaatcaaca
    16741 ataaaaaaac ctgaatatat taaaaaaagt aaacgagtag gatgcggtca gggtagtggt
    16801 catggtaaga caagttgcaa agggcataaa gggcagaaag cacgttcggg agcacatttt
    16861 aaacctggct ttgaaggtgg acagatgcca ttgcaaagaa gaattccaaa acgtggattt
    16921 aataatacaa aatttaaaga agaagttcag gtagtaaata ttaatgatat aacaaaattg
    16981 tctgtaaaag aaataacccc tgaagtactt tataaggaag gtttaattaa atcacaattt
    17041 ttaccggtaa agcttttagc agtgggtgac atcaatgatg ccattgctgt tgttgtagat
    17101 gctatatcaa caactgcaaa agataaaatt ataaaagcag gtggtagtgt aacactgcga
    17161 gcataatatg ggacaagagg tcataacact ataatgccaa atgtaatttc aaatatattt
    17221 aaaatacccg agttacgaaa aagagtattg tttacccttt ttattctggt tgtttacaga
    17281 ataggcgctc atatccctat tcctggagtt aatattgagg ctttaagttc atattttgaa
    17341 atggctgcta aaagtcgtgg tagtggtttt gtagattttt ttgatttatt tgcaggtggt
    17401 gcattaaaac ggtttaccat atttgcatta ggaattatgc cttatatttc agcatcgatt
    17461 attatgcagc ttttaacagt tgttatacct gctattgaaa agttatcgaa ggaaggccca
    17521 gaaggttata aaaagataaa tcagtatacc cgttatggca cggttctctt atgtgcattg
    17581 cagggttttg gtttgactat gtggatgaag tcaatgactt caccaaaagg tgcagtagtc
    17641 ccacaggatt ctggtatagg atttgtactg gtagctgttt tggcaattac taccgggaca
    17701 atgatattga tgtggttagg tgagatgata actgaacgag gtatagggaa tggaatatct
    17761 cttattatat atgccgggat tgttgtacgt gctccttcag catttgttga tactatgaat
    17821 aggattatta gcaggagcga tccaattatt gccttgattt taattgccct gtttattggt
    17881 gttattgctg cgtcaatttt attaacatta ggtgtaagaa aaattcccgt acaatatggg
    17941 aagagggttg ttggcagaag aatggttcag atggcatcac aggcaatacc cttacgagtt
    18001 aatgctgcag gcgttatccc tattattttt gcatcatcaa ttattttgtt ccctgcacag
    18061 atcagcaata tgttaggagc tacaaaatat ggcatattca caaagatgca atactggctg
    18121 tcaccagggc atacagcgta tatgattatc tatagtcttt tgataatatt tttctgttat
    18181 ttttatacag ctatacagtt taatcccaat gatttagctg aaaatctaaa aaagtatggc
    18241 ggctttatcc caggaataag accgggacaa aatacagctg attatataat gcatatttta
    18301 aatagaatca cgttgccggg ttcaatattt ttagcattga ttgcaatatt tcctgatttc
    18361 attatacgaa tatggccaga agaagtatct ccggaaatgg catatctctt tggtggtact
    18421 tcattgctta ttattgttgg tgttgctctt gatacattaa aacagatcga gtcacaattg
    18481 cttatgcgtc attatgatgg attctttaaa aagggtcgta taaaaggtag aagatattaa
    18541 atttttagct atactatgtt tttagtatgt ttctatattg aggtattttg gctatatata
    18601 tttacaatga ggacgagatt aaacgggtag aagatgcgtg cagaataaca gcagcggttc
    18661 tggaagagtt aggtaaaaat gttgttgagg gtattagcac ttttgagtta gatagagtag
    18721 cacgtgattt aataagaaaa agcaatgcaa ttccagcatt tttaggatat aaggggtatc
    18781 cagctgcaat ttgtacatca gtgaatgaag ttgtagtaca tgggatacct catagaaatc
    18841 agattttaag aaaaggtgat atcgttggta tagatattgg tgttaagttt aaagattttt
    18901 ttggtgatgc cgctaagacg tttatcgttg ggaaggtaaa caacaaagta aaaaaattac
    18961 ttgaagttac caaagaggcg ctggaattag gtattttaca atgtaaagac gggaatcacc
    19021 ttactgatat atcccatgta attgaatccc atgttgtgca gtggggattt cagcctgttc
    19081 atgagtttgt gggacatggt ataggtagaa atatgcatga ggaaccttct ataccaaatt
    19141 ttggtaaacc ggggaaggga cctataataa aaacaggtat gatctttgca atagaaccaa
    19201 tgattaatga aggtacgtat caggttgaaa ttttggatga tggatggaca accataacaa
    19261 aagacaggaa aaattcagct cattttgagc atacggttgc tgttgtacat ggaagtccaa
    19321 aaattttaac aatggtttag aaaacaaaag aggagtataa agccatatgg ccaaagagga
    19381 acctattgaa gttgaaggag aagttgttga agcgcttccc aatgctatgt ttagagttaa
    19441 gcttgataat ggtcatgtgg tactggctca tatttcaggg aagatgcgaa tgcattatat
    19501 cagaatttta ccaggcgata aggtaactgt tgagctttcg ccatatgatt tgagtagagg
    19561 cagaataacg tatagagcaa aatgatatat gagagcttga ggtaaactat gaaagttaga
    19621 gtttctgtta aaaaaatatg cagtgattgt aaggttataa aacgacatgg ggttgttcgt
    19681 gtgatttgta aaaatccacg gcataaacag cggcaaaaga aaagaacagc gaaataatac
    19741 aatatagagg tttatatggc acgaattgca ggtgttgatt taccaaataa taaacgtgta
    19801 gaaatagcac ttacctatat ttatggtata gggcttccct cttctctgaa gatcttggaa
    19861 gaagcaaggg ttaatcctga cacaagggtt aaggatctta ccgatgaaga gattaaccag
    19921 ttacgaaaaa ttattgaaaa aagttataag gttgaaggcg atctgcgtac acaaattgca
    19981 atggatataa agcgtcttat ggatattggt tgttataggg gtatacggca tagaagagga
    20041 ttgcctgtcc gtgggcaaaa tactaaaaat aatgcacata ctagaaaagg caatagaaaa
    20101 gtttctgttg gtggcaaaaa gaaaaaatag taactgaata acagaggtat acagtgaagc
    20161 agaaaagaac gaaaaagcag gaaaaaaaga atataccggt aggaaaagcg tatattcagg
    20221 ctacgtataa taatactatt ataacaatca cagatatgca gggtaatgtt gtatcgtggg
    20281 caacatctgg tggcgagggt tttagaggat caagaaagtc aacacctttt gctgctcaaa
    20341 tggctgctaa agctgcagca caaaaagcaa ttgataatgc taatatgcga agtatagaag
    20401 tatatgtaaa gggtcctggt atagggcggg aggctgctat ccgctcatta taccaggcag
    20461 gtttaaatat atcaaagata aaagatatta cacccatacc acataatggg tgtagaccac
    20521 gcaaacgaag aagagtataa cgtgaggttt taatctatgg caaaatatac tgggcctttg
    20581 tgtaaattat gtagacggga aatgactcca ttgatgctga aagggagaag gtgcttaacc
    20641 gataagtgtg ctattaagca taaaaagtat cctcctggac cacctaaaaa gcgtcgaatt
    20701 aaattatcgg attacggagt gcagcttcgt gaaaagcaga aaattaaacg aagttatatg
    20761 cttttagaaa cacagtttaa acgtgttttt gaagaggcta cccggttaaa aggagttaca
    20821 ggggaaaata tgcttgtatt gcttgaaaga aggcttgaca atgttgtata cagaatggga
    20881 tttgcatctt ctcgtttgca gtctcgacag ttggttcaac atgggcatgt aataataaat
    20941 ggcaaacgtg ttgatatacc atcatatcag gtaaaggtaa atgatattgt tgagattaaa
    21001 gaagatttta aaaataatgc tattttagaa gatgcaataa aattgtcaaa agctgttgga
    21061 tcggttcctc agtgggtaga agttgattat gaaaagagga atgggaaaat tatcaggttg
    21121 cctgagcgaa aagatattga gcctatattt aatgagcaag ctgttgttga gttatattct
    21181 aagtaagact ttagaatagt tatgtgttta tttactggag gtgataatgt cttataagaa
    21241 cctattaaaa ggttttcgaa gaccatcgaa aattgttttt gagcatgatg aagtaaacca
    21301 ggattatgga agatttattg ctgaaccttt tgaacgtgga tatggcttaa caataggtaa
    21361 ctcgctacga agggtattat tgtcatcaat tgaaggagct gcaataactg cagtaaagat
    21421 tcagggagta ccccatgaat ttatgacgat tgatggtgtt gtagaagatg ttacgcgtat
    21481 tttattgaat ttaaaacgtt tgcgatttag gtatgcaagt gagttgccca aggtactcca
    21541 tatagttaag gatggtgaag ctgaactgac tggagcggat ttttcagttg atagcgatgt
    21601 tgaggtaatg aatcctgata
//
LOCUS       DAOSOX010000021        21013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2525_length_21013_cov_8.986831, whole genome shotgun sequence.
ACCESSION   DAOSOX010000021 DAOSOX010000000
VERSION     DAOSOX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21013
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_21013_cov_8.986831"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLX22_02945"
     CDS             complement(<1..1293)
                     /locus_tag="PLX22_02945"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT18894.1"
                     /translation="MNLYTSNSLNFLAERLGHLLFEKKKDPLVQETIIVQSIGMQRWI
                     SLKLAQQFGVLLNCNFPFPQKFAEDVFSAVIPEYQLSSLYEPQSLVWTIMRLLQHCIN
                     EKPFQHIKQYLTVSDAISYIRLFQLSQRLAYMYDEYIVYYCNDIVQWENKNNADEWQI
                     MLWKYIYHAISIDPHDCHKAHLLNYFLEALKNNNTKKAMKAALPERIFLFGITALPEY
                     YIMLFKALSSFIDVYIFQLNPSKEYWFDIRSEQSITRIKKKLLAKKQMVDDFHFETGN
                     PLLASSGTLGREYIGLLLDYDAAENINDETQYQIGNNDTLLGHIQSDICCLINRGKDS
                     NAAKIPIKSNDDSITIHSCHSPLREVEVLRDYIYNILNNENIVPNDIVVMAPDIDKYS
                     PFIAAVFDEVMLKKEGLPLLPYTIADQSFKTASRYISAF"
     gene            complement(1469..1768)
                     /locus_tag="PLX22_02950"
     CDS             complement(1469..1768)
                     /locus_tag="PLX22_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18895.1"
                     /translation="MWIYMNDCFLSIIENDEQNLTVCAQRKEDITRVFPEADVIEMPE
                     NDYGCRTIVTRRDVAMTIAKRILSIDYYNFKDSVKENDRKHIYADIWGNSLQLRK"
     gene            complement(2339..3376)
                     /locus_tag="PLX22_02955"
     CDS             complement(2339..3376)
                     /locus_tag="PLX22_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT18896.1"
                     /translation="MNYLLLAILGTMVILIILVLFLLLRNQPSDVASQINAAIANQLL
                     TFQSAIHNELNSTRQELSQSKDTIAQHTLKTIESINKIGQTIHTITQQQEEAQKLGQS
                     LKDLLQSPKLRGNYGEAILEEMLDRVLPKGIWEMQYAIEGREKVDAVVKMKDMIIPID
                     AKFPRDNYQKYLQAQSAEEKRNYWDKYVNDVKTQIKSISAKYVKPEKGTSEFALMFIP
                     SEAIYYETIAEKNYLGVPSDIYEFAQDQKVIPVSPNTFYAFLQLIVVGSRNLEIIKSV
                     KKIQGGLAELEKSFELFYNKYEDIGKNIEKAQEAYRIGDSHIKRYKNRLDSTLSLEEF
                     AVNDTALPDKK"
     gene            complement(3666..3881)
                     /locus_tag="PLX22_02960"
     CDS             complement(3666..3881)
                     /locus_tag="PLX22_02960"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOT18897.1"
                     /translation="MNKQKLLKPVNVMMAIDFVCLSGTAMLDDAIPKDIYGIIHPIFG
                     YIFVLCVVFHVFLNWAWVKSNFIITKK"
     gene            complement(4087..4329)
                     /locus_tag="PLX22_02965"
     CDS             complement(4087..4329)
                     /locus_tag="PLX22_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18898.1"
                     /translation="MIKNTKRLQQFEKEFVQKNSLSHLQALKLLDGMWEMGMTLGVLP
                     PDDPWEGIEVDIRVARIVNCFKEEISFGMYATLKSW"
     gene            complement(4326..5450)
                     /locus_tag="PLX22_02970"
     CDS             complement(4326..5450)
                     /locus_tag="PLX22_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18899.1"
                     /translation="MSTIEVKEVKDSKMMKQFIMFPWTSKIYENDPAWVPPIIGDMKK
                     LFNPKKGYFYDIGEVAYFLAYKDGVPAGRITAHVNRMYEEKYNKETGFFGFYEAIDDL
                     DVARALFAVAEGWLKKKGKTRVEGPQSFSIYDSVGFEVFGEEIMPVVGLLHFAPYYKD
                     HATACGYTKCIDWHCFLVKKIDDFKPYLKDVRENIMKNQDVKYVYYTPKELKKRAKEI
                     HEIFNRAWDGNWGHLPLTDKQFKMLVDELKLVAVPELIVFAEKDGRTIGFIVSLPDMN
                     PALKILNGRLYPWRLIKALRSIKKVKRIRTIIMGVLPEYRGQKIDDVFYLFTIENGIR
                     LGYVESDCSLIVETNQKMIGALKPLKGQIYKTYRIYEKHI"
     gene            complement(5798..6379)
                     /locus_tag="PLX22_02975"
                     /pseudo
     CDS             complement(5798..6379)
                     /locus_tag="PLX22_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(6383..7390)
                     /locus_tag="PLX22_02980"
     CDS             complement(6383..7390)
                     /locus_tag="PLX22_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT18900.1"
                     /translation="MFNPLKIFKNKKVGLALGSGGAKGIAHVAVIDYCEKNAIPINII
                     AGSSIGAVIAALYAYGSLKEFVSFLRLVKSNDIFSYFDPVFPKTGLIQGRRMIELLAK
                     FIPKSVSIEDLKIPVAIVATDFYSGNPVVFKKGNVLEAIRASISIPGIFVPVRYMDTV
                     LLDGGVANPLPIDVVKQMGAGITIAVNLHPTIKVKKAKIAAMNSLQELSPKEENSSND
                     IIEHVDKEPVDTLSKITQRSGGWINKAKQWLGMNSEGNAAKDNIPNIFEIVSGTIDIM
                     EFMNTTLMITYNKPDVLIEPDLLQVSTLDFTKVDMLLQEGYAACERVQKDIKHKVLRW
                     V"
     gene            7851..8477
                     /locus_tag="PLX22_02985"
     CDS             7851..8477
                     /locus_tag="PLX22_02985"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT18901.1"
                     /translation="MALRRVISVGASEDRLEKLIQKRLKPDVNKELIDKRIWDLFGEV
                     WCVMFTDLAAFSRKVATYGIIHFLQTIYESQRLLVPIIEEYDEILLKTEGDSMLVIFR
                     NVVKGLECCKTMHQVLYDYNNNKHEEDHIVLCVGLGFGTMLRIGDEDVYGAEVNAASK
                     LGEDIAEAWEVLVTNSVKENANNISNITFEPIDFIPPGAQSAYKVIFK"
     assembly_gap    8554..8653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8683..10344
                     /gene="ilvD"
                     /locus_tag="PLX22_02990"
     CDS             8683..10344
                     /gene="ilvD"
                     /locus_tag="PLX22_02990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT18902.1"
                     /translation="MISDRVKKGIEKAPHRSLFKALGLTDDELSKPLIGVVNSFNEII
                     PGHIHLKNIVEAVKKGVIAAGGTPIEFPTIGVCDGIAMNHIGMKYSLGSRELIADSIE
                     IMAKAHAFDGLVMVPNCDKIVPGMIIAAVKVDIPSIIVSGGPMLPGNYKGKAIGLDEV
                     FEAVGKAAKGLISKEDLYEIECSACSGAGSCAGLYTANSMNCLTEALGLALPYNGTIP
                     AVMADRIRLAKESGKKIVELVKKQLTPKKIVTKKAIENALSVDMAIGGSSNTALHIPA
                     ISHYAGIPIRLKDIQQISERIPHLCSIAPAGPHHMVDLHYAGGMPALINELLKHGLIH
                     GNAMTVTGKSIAQNVKDKTIFDTSVIRNVTKPVHKDGGLAVLTGNLAPDGCIVKQAAV
                     DPSMYKHSGPARVFDSEEEAVKAIMGGQIQKGDVIVIRYEGPRGGPGMREMLTPTSVI
                     AGMGMDKEVALITDGRFSGATRGSSIGHISPEAASGGPIAIVKDGDIIEIDIPAKKLT
                     LKLDDKEIKARLAKWKAPKPKITSGYMYRYSLMVSSADRGAVFES"
     gene            complement(10419..11204)
                     /locus_tag="PLX22_02995"
     CDS             complement(10419..11204)
                     /locus_tag="PLX22_02995"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT18903.1"
                     /translation="MEQQVKYVVFDIESIPNAPLIKQVKYPHLSIGEHDAVIKFQEEI
                     LNVSDGSTWFIPVTFQLPVIVSMLEVDEKFFPIRMHTLDEPQFRTEVIVNQFWEAIES
                     IYNDASYVTFNGRGFDFPLMELMAFRFGVTAKRHFKDKFASRFRFGTKHIDIHDWLSN
                     YSAIRMQGGLNLLAKVIDKPGKVEMTGDDVYDLFKEGKLQSIHEYCISDVMDTYFVFL
                     RTRVMIGELSREREGVIIQHTRDFLEKNKSAVKGFEQYLTNWK"
     gene            complement(11370..11840)
                     /locus_tag="PLX22_03000"
     CDS             complement(11370..11840)
                     /locus_tag="PLX22_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT18904.1"
                     /translation="MKKIMMLTLCIVFSLAIISDSSIAQDTVKKGTKEYIADLSSNDE
                     AVVVAAEEWIADNKEKSALSRLQELLRSDNRVKVRLYAAIALGEIADESAAESLNNAL
                     LNDPSADVRYTALLGIARIGSKDSYDAIQKARESEQDPFIKDLIQKMEDKLKGK"
     gene            12327..13262
                     /locus_tag="PLX22_03005"
     CDS             12327..13262
                     /locus_tag="PLX22_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT18905.1"
                     /translation="MNTILIVDHTDYKNILKDYFSSYYSVLFADCAFDAISLLNMHDI
                     DLVISQVALPGDNAFDLYNYLQNHYTYIPAIMITESNIDIFFDEIFRQGIGNVLKIPV
                     NKTEFVQLVDKIISRNNVFGLSNYIHNIIETNSIRINASKQIQSGVDTILATIESWGF
                     TIKNKVVLSLILNEMIINAVYHSHGYTHEKEMRVPIELKEGQYVDISFCHNASTYGIA
                     VTDYQGTLSKGKILESIHKAIEQEQLILHAAKTGEDITDKISETGRGIDLVRKLASEF
                     YFVIQKNKRTEIILLFESSSSIQTSSSLKIIEDMR"
     gene            complement(13273..14439)
                     /gene="ftsZ"
                     /locus_tag="PLX22_03010"
     CDS             complement(13273..14439)
                     /gene="ftsZ"
                     /locus_tag="PLX22_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT18906.1"
                     /translation="MFKFDEEPQMNTIIKVVGVGGAGNNAVNRMIATGLEGVDYIVVN
                     TDSQQLKASLAQTKIQIGTKLTRGLGAGADPSIGREAALEDRDKLEKALKGADMVFIT
                     AGMGGGTGTGAAPIVAEVAKESGALVVGVVTKPFRAEGRRRMQHADEGIKNLKEKVDT
                     IIVIPNDLLLKIIDRSTPMDHAFKLADDILRQGVQGIADIIMVTGLVNVDFADVRTVM
                     KETGDAIMGVGIGVGENRAIEATQMAINSPLLEESGIEGAKAVLLNIAGGSDMSLHEV
                     NEITEIINKQVDPDANIIFGATIDPALDDKIRVTVIATGFDRKLNMLNRPNELDRATG
                     TPLTVIEGKNHKERIEKKVPAQMKSFTLDYEKRRVKDYSHEDLDIPAFLRRNAE"
     gene            complement(14512..>15444)
                     /gene="ftsA"
                     /locus_tag="PLX22_03015"
     CDS             complement(14512..>15444)
                     /gene="ftsA"
                     /locus_tag="PLX22_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT18907.1"
                     /translation="NRTITPVEMKRVIEAAQAIVIPMDREIIHVLSKEFAVDDQTGIK
                     DPIGMSGVRLEAEVHIITGSTTSIQNLVKSVNKAGFVCNDIVFSPLASAESTLSRDEK
                     DLGVALVDIGGGTTDIMVFIEGGVAYSAVLGIGGIHVTNDISIGLRTPIDSAEVIKKK
                     YGCAVVDLVDASETIEVPSVGGRAPRRLFRHELAQIIEPRVIEIMEMIDNELVKSGKK
                     EILAAGVVLTGGGSMIEGTVDAAERVLNMPVRVGLPENIVGLKDVVSTPMYANGVGLL
                     RYGAKMGQLRQTRKTHGGLKSLKDKLRQIFNDYL"
     assembly_gap    15445..15544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15578..16267)
                     /locus_tag="PLX22_03020"
     CDS             complement(15578..16267)
                     /locus_tag="PLX22_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18908.1"
                     /translation="MKSNLYTASMIVSLLLFPLYGYASVEAISTVKLYGLKYVTKFEI
                     LNNAVISKDDRFFVDMKKLNNSLQNNPMIESYTVDTNNNILTVKVKEKDILAIVLIEK
                     GNDTIPIEVDSHLKIISTYRVHAYDRPIIKITQDEVYHNKLSPRIIKILMIMNKLKNV
                     QGQAIGRISVIDCINPFVSYVSIHGLQTRYIWDETYEGISLMYSVPGLLDSKSHCPGN
                     GVVTQKRIVVW"
     gene            complement(16264..17646)
                     /gene="murC"
                     /locus_tag="PLX22_03025"
     CDS             complement(16264..17646)
                     /gene="murC"
                     /locus_tag="PLX22_03025"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT18909.1"
                     /translation="MFRKSKKMHFIGIGGIGMSGIAEILINLGYDVSGSDLRESEQTK
                     RLRQLGATIYIGHYPSNIKDYHVVVTSSAISKNNPEIIEAKKRKIPVIHRSEMLAELV
                     RLKHGIGVAGTHGKTTTSSMLAYILYHGGLNPTAVVGGKVLNFGSNARIGEGQYFVFE
                     ADESDGSFLKLLPTIGIVTNIDADHLDHYKYFEGIKEAFITYMNNIPFYGYSVVCMDD
                     PVVAEVLPRIERPYVTYGFNTGAHFKATNIKLLNGSTRYSCYYNDKLLGEIVLNLLGK
                     HNIINSLAVVAVALELGLTFGVIAEAIAQFQGVGRRLEKIGETNGIIVVDDYGHHPTE
                     IRATLDALKQLGKRVIVVFQPHRYTRTQYLYDEFGQAFTMADELFLTEIYPAGEEPIE
                     GVSSNLIQQSVKKHEGRDVVVIPHLDQVPDYISPIVREGDVIVTLGAGDIYKVGHAIL
                     SMLDKKGVVQ"
     gene            complement(17659..18768)
                     /gene="murG"
                     /locus_tag="PLX22_03030"
     CDS             complement(17659..18768)
                     /gene="murG"
                     /locus_tag="PLX22_03030"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOT18910.1"
                     /translation="MKTPVLISGGGTGGHIMPGIALYEELKSKGYEVFLLVGTGDVKF
                     SSLNSVENIQVYNAPTMWKNIVNFPVFTIRFISAMLWTMKFIAKNNIAAVIGMGGYVS
                     APSLVAAMIKKIPIYLCEQNVVPGMVTNFFAKRARAIFTTFEATTTYFDVESTGKIVH
                     AGNPIRKQVLTPVEKDAALKRFNMIHCKKVILVIGGSQGALKLNELTYDMLVSYPDRF
                     ADVGIIWSTGTYSYQQFKEKLHTIKHASIYLAPYITDVENSYAACDIAISRAGAGVMM
                     ELAAWGIPSILIPYPFAAKDHQKKNAQEFVKAGAAEMINNSDATAQVVATLLFDLLDN
                     ETLLRKMSAKAKSIAKHNAAEIIINYIIKDSDLKK"
     gene            complement(18765..19922)
                     /gene="ftsW"
                     /locus_tag="PLX22_03035"
     CDS             complement(18765..19922)
                     /gene="ftsW"
                     /locus_tag="PLX22_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT18911.1"
                     /translation="MDVKAVIDTRRKGEPEIALFIVSFILIGIGIAMSYSASAIFAER
                     VFGDSLYFLKRQLVWCCLGFFIMMIVQHIDYRYYQHYTRIMLLFTMIALILVLIPGVG
                     ITVKGSSRWLGYGPLQFQPAELVKLFVVIYFAKVFSYDNHREINVLQLLLPLLVVGIF
                     FLLIMLQPDFGTAINLAIISVVLLFVSGFPLIYMIGLALLSVPMFYLLIYQVEYRRQR
                     ILAFFDPWSDRFGKGYHIIQSFIAFKLGGMLGAGLGSGTQKIKRLPEPHTDFIYAVIA
                     EEAGLLGTFSILILYLFFFYFGVKIALKAPDEYGRLLAMGLTLLITVQAFLNISVVIG
                     LLPTTGIPLPFISYGGSSLLVNMMATGILLNISKYREVVPRDIKFDKEVLV"
     gene            complement(19913..>21013)
                     /gene="murD"
                     /locus_tag="PLX22_03040"
     CDS             complement(19913..>21013)
                     /gene="murD"
                     /locus_tag="PLX22_03040"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT18912.1"
                     /translation="PVISEIELAYRYCKAAIVGVTGTDGKSTVTMLIAHCLTQLGFDA
                     HPGGNIGIPFISLVDSLHEDSVAVIELSSFQLETVKTFKPDVAIITNLTPDHLDRYNS
                     LYDYFNAKMNIAINQCKDDYFIFNRDDEFIVNNMPALQSQQHSFSLLGKADCFYSNGA
                     IVMDSNGETTLVAYASNMKIAGLHNVQNAMAAILASTFIGKKFGNNIDLKVLEEALYS
                     FKGLPHRVQNIGEYKGRIFINDSKATTTGAVIMALKSLQDNVILILGGRAKGDDYSRL
                     KPVIKGKVKALVLIGETKDEFASIFSDIPHIKAFSMEDALIKAMKLSAKGDTILLSPA
                     CASFDMYKNYEQRGNHFIEIYSKLVRGELAWM"
BASE COUNT         6397 a         4370 c         3309 g         6737 t
ORIGIN      
        1 aaatgctgag atgtatcggc ttgctgtttt aaatgactgg tcggcaatag tgtatggcaa
       61 caacggcagt ccttcttttt ttagcataac ctcatcaaac actgctgcaa taaatggcga
      121 gtacttatct atatcaggtg ccatcacaac aatatcattg ggtacaatat tttcgttatt
      181 caatatgttg tagatataat cgcgtaatac ttcaacctca cggagtggag aatggcagga
      241 atgaatggtg attgaatcat catttgattt tattggtatc ttcgctgcat tggaatcctt
      301 accacgattt ataaggcaac atatatctga ttgtatatga ccaagcaatg tgtcattgtt
      361 cccaatctga tactgcgttt catcgttgat attttctgca gcgtcatagt ctaaaagcaa
      421 accaatatac tccctgccca gtgtgccaga tgaagctaac agcggattgc ctgtttcaaa
      481 atgaaaatca tcaaccattt gtttttttgc cagcagcttc tttttgatac gggtaatgga
      541 ttgctcggaa cgtatatcaa accagtattc ctttgaagga ttgagctgaa atatatacac
      601 atcaatgaat gaactcaaag ctttaaaaag cattatataa tactcgggta atgctgttat
      661 accaaataaa aatatacgct ccggcaacgc agctttcata gcctttttag tgttattgtt
      721 tttcagagcc tctaaaaaat agtttaacag gtgtgcttta tgacaatcat gggggtcaat
      781 tgaaattgca tggtaaatat atttccacaa catgatctgc cattcatcag cattgttttt
      841 attttcccac tgcactatat cattacaata atatacaatg tattcatcgt acatatacgc
      901 taacctttgt gaaagctgaa acaagcgtat ataacttatt gcatctgaca cagttaaata
      961 ttgtttgata tgctggaatg gtttttcatt gatacagtgc tgcaacaatc gcatgattgt
     1021 ccacaccagt gattgaggct catacaaaga cgacagttga tactcaggaa tcactgcgct
     1081 aaaaacatcc tcagcaaatt tttgcggaaa cggaaaatta cagtttaaga gtacaccaaa
     1141 ctgttgagca agtttcagcg aaatccatcg ctgcatgcca atactctgaa caatgatagt
     1201 ttcctgtaca agcgggtcct tctttttttc aaaaagcaaa tgccctaacc tttcggcaag
     1261 aaaattgagg ctgttgcttg tatataaatt catagtatgc tcaatcacct gtatagtatt
     1321 tgtgtacgat agttactatg gaatgtacac gcttcttcca taaaatatgc cattggtaaa
     1381 tcccatgtca ataataaata catccatgaa gcttttttgc agtaaatgtg ttttgctatt
     1441 aactattata acttcttata atagtgtatt atttcctcaa ctgcaaggag ttaccccata
     1501 tatctgcata gatatgcttt ctgtcatttt ccttaacact gtccttaaag ttgtaataat
     1561 ctatactcag tatccttttg gcgatagtca ttgcgacatc cctgcgtgtt actattgttc
     1621 tgcacccata atcgttttcc ggcatttcaa tgacatctgc ttcaggaaaa actcttgtaa
     1681 tatcctcctt cctttgtgca caaaccgtta agttttgctc atcattttct attattgata
     1741 aaaagcaatc attcatataa atccacatta tagtacctcc ctgttacaat taaactattt
     1801 ctctattgtc attccgggct gacccggaat ccaattaatt atcgtgagat cctgaaacca
     1861 ggtcataaca tagttcagga tgacatgccc tctgttttca ttctgagaga gcacaggcga
     1921 ccggagaatc tatcctttca taaaacaacg agattcttca cttcgcatag catgacagac
     1981 aaattttcgt cgcgtagctg tcaggttgct gtttatcagt gcctgcaagc tatccatagc
     2041 tacaccctcc tgtactgtgt gcagtaataa tagtatatga ctcatttccc aaaaaattta
     2101 tagaaacaaa attgcattat tataaaaaca atacgtacat gtatgtttta atatacatac
     2161 gtggtgggac atataatgtc ccttttttca ttttttttta ttttgctatt gaagatacgg
     2221 gaatagcaaa aatattgctc ttgagtggga ttggttcttt tacaaaacaa ataaaaaaca
     2281 gtataccgac catcctgcaa tgtaatgtta taatagccgg tttacttttc aatgtcaatt
     2341 attttttatc tggcagagca gtgtcgttta ctgcaaactc ctcaagactc agggtgctat
     2401 ccaatctatt tttgtaccgt tttatgtgac tatcgccaat acgatatgct tcctgagcct
     2461 tttcaatatt tttaccaata tcttcgtatt tattataaaa cagctcaaat gatttttcaa
     2521 gctcagcaag cccgccttga atctttttta cactttttat aatttccaga tttcgtgaac
     2581 caaccactat aagctgcaaa aatgcataaa atgtattggg gcttaccggt ataacttttt
     2641 gatcctgcgc aaactcatag atgtcacttg gaactcctaa ataatttttc tcggcgatag
     2701 tttcataata gatagcctcg gatggtataa acattaatgc aaactcagaa gtccccttct
     2761 cgggctttac atatttagcg ctgatacttt taatctgtgt ttttacatcg ttgacatatt
     2821 tgtcccagta atttcgtttt tcttctgccg actgagcctg caaatatttt tgataattat
     2881 cacgaggaaa ttttgcatca atggggatta tcatgtcttt catcttcacc actgcatcaa
     2941 ccttttcacg cccctcaatg gcatattgca tttcccagat tccttttggc agcaccctat
     3001 ccagcatctc ctctaaaatt gcttcgccat aattaccccg caacttcggc gactgcaaca
     3061 aatccttcaa tgactgtcct aacttctgtg cctcttcctg ctgctgtgta atggtatgga
     3121 ttgtctgccc aatcttgttt attgattcaa tagtttttaa ggtatgctgt gcgatagtat
     3181 ctttggattg tgacagttcc tgcctggtag agtttaattc gttatggatt gcagattgaa
     3241 atgttaacaa ttggttggca atggctgcat taatctgcga agcaacatca gatggttgat
     3301 ttcgcagcag taaaaataaa accagaatga ttaatattac cattgtgcca aggatagcca
     3361 gtaatagata attcataatg atatagcccc caaaaattaa ttattattca gctttacaaa
     3421 atttgacaat ttacgctaca agaaaaaaca ctgaagaagt gcttcactca tcatgaaata
     3481 gtattatttc gctatgtcat tacaggcttt attcatgaat caaaatcatt atacgatatc
     3541 ctgaaaataa ttcttaaaat cagtcaagga catgattcag catggcagtt gtgttggctt
     3601 ttgatgccag ctttttaaat taaggcgtaa gatagcaact taataatacg acaaaataaa
     3661 taattctact tttttgttat aataaaattg ctcttaaccc atgcccaatt taaaaaaacg
     3721 tggaatacca cacatagcac aaatatatat ccaaatattg gatgaataat tccataaata
     3781 tcttttggaa tagcatcatc aagcatggca gttccagata gacaaacaaa gtctatagcc
     3841 atcattacat tgactggctt aagcaatttt tgtttattca taaaaattct cctttttatt
     3901 tgatgtatgt ccattccaaa acttcttccc ttttttttat tttttatata atctaccact
     3961 ctgctaatca tcaaatgtga ttataatatc ttactataat tttttttatt ccacggctct
     4021 caatatctct ttttttgaaa tcttataggc ctttttcatt agtccttgca taaaaaacta
     4081 tatgccttac catgatttca aggtagcata cataccaaaa cttatttctt ctttaaaaca
     4141 atttactatc cttgcaactc taatatcaac ttcaatccct tcccatggat cgtctggcgg
     4201 aagaactcct aaagtcattc ccatctccca cataccatcg agcaatttta atgcctgaag
     4261 gtggcttaag gagttcttct gaacaaactc tttttcaaac tgttgcaagc gctttgtgtt
     4321 tttaatcata tgtgcttttc gtatatgcga taggttttat atatttgccc ttttagcggt
     4381 ttcagcgctc ctatcatctt ttgatttgtt tcaacaataa gcgagcaatc agactcaaca
     4441 taacccaacc gtattccatt ttctattgta aacaaataga acacatcatc aatcttctgt
     4501 cctcgatatt caggcaatac tcccatgatg atagttcgta tccttttaac cttttttata
     4561 gaccgcaatg cctttatcaa tcgccaggga taaagcctgc cgttcaatat ttttaatgca
     4621 gggttcatgt caggtagcga aacaataaaa ccaatggtcc tgccatcttt ttcagcaaat
     4681 acaataagct ctggtactgc aacaagttta agctcatcaa caagcatttt aaattgcttg
     4741 tcagttaaag gcagatgccc ccagttgccg tcccatgctc ggttaaaaat ttcatggatt
     4801 tcttttgcgc gcttctttaa ttcctttgga gtataatata catatttaac atcctgattt
     4861 ttcatgatgt tttcgcggac atcttttaaa tacggtttga aatcatctat cttttttact
     4921 aaaaagcaat gccagtcaat gcacttggtg taaccgcaag cggtggcatg atctttatag
     4981 tacggtgcaa agtgaagtag cccaaccact ggcataatct cctcaccaaa cacctcaaat
     5041 ccaaccgaat cataaataga aaagctttgc ggaccttcta cgcgcgtttt gccttttttc
     5101 tttaaccaac cttccgcaac agcaaacaat gcacgtgcaa catccagatc atcaattgcc
     5161 tcataaaaac caaaaaagcc tgtttcttta ttatattttt cctcgtacat ccggttaaca
     5221 tgagctgtaa tccttccggc aggcacacca tctttatacg ctaaaaaata ggcaacctcg
     5281 ccaatatcgt aaaaataacc ttttttggga ttaaaaagtt ttttcatatc accgatgatg
     5341 ggaggaaccc acgccggatc attttcataa atctttgaag tccagggaaa cataataaac
     5401 tgtttcatca ttttactgtc tttaacttct ttcacctcga ttgttgacat aaataccccc
     5461 acaaaaagtt tttaaataag ttgttatata ttgtatatca tttaatcaaa agctacagct
     5521 ttttccttat aataaataat acatttatgc ttactccata acaagaaaat ttttattaaa
     5581 agcaacagtg cttaaagcaa ccagctactt acatactcta tgttcgatag ttactgttaa
     5641 tccattatca gtaactatcg aacaaaataa tttaaaacaa atttccctgt acctcatcat
     5701 cacgtttatt ctcagttgta tcatgtaaac ttaactcggg taatgttttt atttttttag
     5761 caatatcggc agaagaaact tcaccatgta aataagcaag gacattcttt atgtcctccc
     5821 acaacagacg cttcagtggg ctggcatcac caccgttgcg caaaatatac gatgggtgaa
     5881 atgttggcat aaccggaaaa ccgttataaa gctgccacac acctctgatt tttgttatgc
     5941 cttcttttga attgagtaaa aattttgtag atgggttgcc cagcgttact ataacttctg
     6001 gttgtatgat gtcaatctgc tgttttaaaa atccaccaca ggcagctgtt tcttcatcat
     6061 caggtggacg gtcacgcttt aactccatat taaccgttgg tctgcactta acaacattgg
     6121 ctatatacac atcactgcgt ttaagcttca tccctttttc aatcaccgct gttaaaagct
     6181 gtcctgcttt accaacaaag ggcctgccct gcgcatcttc gtctttgccg ggaccctcgc
     6241 caataaacat gacacgtgct tcaggattac cttcgccaaa gactatggtg ctacgcgttg
     6301 catgcagctt acaccgcttg cagttgccaa taccgtgaac acgcaaatct tctaactgtt
     6361 gggcttttgt taatgccata gtctataccc atcgcaaaac tttatgttta atatcttttt
     6421 gcacccgttc acatgctgca tacccttcct gcaaaagcat atcaactttt gtaaaatcaa
     6481 gcgttgaaac ctgcaaaaga tcaggctcaa tcaacacatc aggcttatta taggttatca
     6541 taagtgttgt attcataaac tccatgatgt caatggtacc ggaaactatc tcaaaaatat
     6601 ttggtatgtt gtcttttgct gcattgccct cactgttcat tcccaaccac tgttttgctt
     6661 tatttatcca accgcctgat cgctgtgtaa tcttcgataa agtatcgact ggctccttat
     6721 caacatgttc aataatatca tttgaagaat tttcttcttt gggcgatagt tcctgtaaac
     6781 tgttcattgc cgcaatctta gcctttttta ctttaatggt tggatgtaga ttaaccgcta
     6841 tcgtgatacc ggcacccatc tgttttacca catcaattgg cagaggattg gcaacaccgc
     6901 catccagtag cacggtgtcc atatatctaa ccggaacaaa aatcccgggg atggatatgc
     6961 ttgccctgat tgcctcaagg acatttcctt ttttaaaaac aacaggattg ccactgtaaa
     7021 aatcagtagc aactatcgct accggaatct ttaaatcttc aattgaaaca ctttttggta
     7081 taaattttgc taaaagttca atcatccgcc tgccctgtat caatcctgtt tttggaaata
     7141 caggatcaaa atatgaaaat atatcattgc ttttaacaag ccttaaaaat gacacaaact
     7201 ccttaaggct gccatacgca tacagtgcag caataaccgc accaatgctg gaaccggcga
     7261 ttatattaat ggggattgca ttcttttcgc aataatcaat aacagcaaca tgcgctatcc
     7321 ctttagcacc accactgcca agtgccagac caactttttt atttttaaat atttttagtg
     7381 ggttaaacat agtataatta tatgatatac atctaatgtg atgtatagtt gtaaagggtg
     7441 taatagcttt ttgtcaagag gtttttataa taattagtgc tccccaagta tagttcttga
     7501 cattatacat tttttatatt tcactatttt cattcctttt ttattatcgt gatcttgtgt
     7561 tatgccatag acaatgtggt gacatgtcat tctgaataga gtgaagaatc tcatcgtttt
     7621 atgaaagaat agattcttca attgctaacg ctttcttaga atgacagcgt gcgaagtttc
     7681 atcccgaaac gtaataagtc atcctgaact ctgattcagg attatttcag gatctcacaa
     7741 taattaattg gattccagat caagccccca atgacggtgg tagtaaagta gatttcgggt
     7801 caagcccgca atgacgataa gtaataatac tatttttgga ggatgaactc atggcattac
     7861 gaagagtaat ttcggttgga gcatccgaag atcgtcttga aaaattaatt caaaaacgtt
     7921 tgaagccgga tgtcaataaa gaattaattg ataaaaggat atgggatttg tttggtgaag
     7981 tatggtgcgt tatgtttact gatttagccg ctttttcccg aaaggtggca acctatggca
     8041 tcattcattt tttacagacc atctatgaat cgcaacgcct tcttgtacca atcattgaag
     8101 aatatgacga aattttatta aagactgaag gtgatagcat gcttgttatt ttcagaaatg
     8161 ttgttaaagg tttggaatgc tgcaaaacaa tgcatcaggt tttatacgat tataataaca
     8221 ataagcatga agaagaccac attgtgttat gcgtaggttt aggatttggc accatgcttc
     8281 gcattggcga tgaagatgta tacggtgcgg aggttaatgc tgccagtaaa ttaggtgagg
     8341 atattgcaga agcatgggaa gttttagtaa ccaattcagt aaaagaaaat gcaaacaata
     8401 tttccaatat tacttttgaa cctatagatt ttattccacc tggcgcgcaa tccgcctata
     8461 aagtaatttt taaatgatgt ctttttttag taaattttaa tttacaatat atatatatac
     8521 tattatgtat acattaatta ttgtctatat atannnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnngtctata tataaaggag gaattttgta ttatgataag cgaccgtgtt
     8701 aagaaaggta ttgaaaaagc acctcatcgt tcacttttta aagcgttagg attaactgat
     8761 gacgagttat caaaaccatt aattggcgtt gttaactcat ttaatgaaat aattcccggt
     8821 catattcatc ttaaaaatat cgttgaagcg gttaaaaaag gtgttatcgc cgctggtggt
     8881 acgccaattg aatttccaac aattggcgta tgcgatggta ttgcgatgaa ccatataggc
     8941 atgaaatatt cattagggtc acgtgagctt atagctgatt caattgaaat tatggcaaaa
     9001 gcgcatgcat ttgatggtct tgtcatggta cctaattgtg acaagatagt tcccggcatg
     9061 ataattgctg ctgtaaaggt tgatatacca tcaatcatag tttctggtgg tccaatgctt
     9121 ccgggcaatt ataaggggaa agctattgga cttgacgagg tctttgaagc tgtaggtaaa
     9181 gctgcaaaag ggcttatttc caaagaagat ctttatgaaa tagaatgctc agcctgctca
     9241 ggcgctggtt catgtgcagg gctttatact gcaaattcca tgaactgtct tactgaagca
     9301 ttagggcttg cacttcctta caatggtact attccagcag taatggccga tcgtatccgt
     9361 ttagcaaagg aaagcggtaa aaaaatagtt gaacttgtca aaaaacaatt aacaccaaaa
     9421 aaaatagtaa caaaaaaagc aattgagaat gcgctttctg ttgatatggc aataggtgga
     9481 tcttctaaca ctgcacttca tatccctgca atatcgcact atgcgggaat ccccatacgg
     9541 cttaaagata tacagcagat atccgaacgc atacctcatc tttgcagcat tgctccagcg
     9601 ggtcctcatc acatggttga cctgcactat gctggcggga tgccagcttt aatcaatgaa
     9661 ctattaaaac atgggctaat ccatggcaat gccatgacgg taaccggtaa gtcaatagca
     9721 caaaatgtta aagacaaaac catatttgat acatcggtta tacgaaatgt aactaaacca
     9781 gtgcataagg atggaggatt ggcggtgctt accggaaact tagctcctga tggttgtatt
     9841 gtcaaacagg ctgctgttga tccttcaatg tataaacaca gtggacctgc aagggttttt
     9901 gattcagaag aagaagcggt taaagcaatt atgggcgggc agatacaaaa aggagatgta
     9961 atcgttatac gttatgaagg accgcgcggt ggtcccggta tgcgtgagat gctcactccc
    10021 acatccgtta tagctggtat gggcatggat aaagaagttg cacttataac cgatggcagg
    10081 ttttccggtg caacacgagg ttcttctata ggtcatatat caccagaagc agcctccgga
    10141 ggccccattg caattgtcaa agatggtgat attattgaaa ttgatatacc ggctaaaaaa
    10201 ttaaccctta aattagatga caaggaaata aaggctcgcc ttgcaaaatg gaaagctcca
    10261 aaaccaaaga ttaccagtgg ctatatgtac cgatacagtc ttatggtttc atccgcagat
    10321 agaggcgctg tttttgaaag ttagaaaata gttaaacaat tactgctcat agcaatggca
    10381 ggtttttacc atgtatattc ctgccattgc aaaacaattc acttccagtt tgttaaatat
    10441 tgctcaaatc ctttgacagc agatttattc ttttccagaa aatcgcgtgt atgctgaata
    10501 ataacacctt cccgctcacg acttaattct ccaatcataa ctcgtgttcg taaaaacaca
    10561 aaataagtgt ccatgacatc agatatacaa tattcgtgaa tggattgtaa cttcccttct
    10621 ttgaagagat cataaacatc atcgccggtc atttcaacct tgccaggctt atcaataact
    10681 tttgccagta aattcaatcc tccctgcatc ctgattgcac tgtagtttga cagccaatca
    10741 tgaatatcaa tatgttttgt accaaatcta aaccttgaag caaatttatc cttaaaatga
    10801 cgctttgccg taacgccaaa cctgaaagcc atcaactcca tcaaaggaaa atcaaaacct
    10861 ctaccattaa aagtaacgta tgaagcatca ttatatatgc tttctattgc ctcccaaaac
    10921 tgattgacaa taacttcagt cctgaattga ggttcatcca atgtatgcat ccgtatgggg
    10981 aaaaattttt catcaacctc aagcattgaa actataacag gcagttgaaa tgtcacaggg
    11041 ataaaccatg ttgaaccatc cgaaacgttg agaatctctt cctgaaattt tataaccgca
    11101 tcatgctcgc caatagataa atgcggatat tttacctgtt ttattaaagg tgcattaggt
    11161 atagattcta tatcaaaaac tacatatttt acctgttgtt ccacggtaac attcctttga
    11221 aaattaatta aaaattacat cttcatgtaa taattcatgt aataatcagc ccacggtcta
    11281 acccgtgggc tgttaatcta ttgcttactg tataccaaca aacttatgta cagcattacc
    11341 gtctaaatag atttttagac gctacgatat catttacctt ttaatttatc ttccattttt
    11401 tgaattaaat ctttgataaa aggatcctgc tcactttcac gggctttctg tatggcatca
    11461 tatgaatctt ttgaacctat ccgtgcaatc cccagcagtg cagtataccg tacatcagca
    11521 cttggatcat taagtagagc attatttaaa ctttctgcag cagactcatc ggcaatctcg
    11581 cccaaggcaa ttgcagcata taaacgcacc tttactcgat tatcacttcg caataattcc
    11641 tgcaaccttg ataatgcaga tttttcctta ttatcagcta tccactcctc tgcagccact
    11701 actactgctt catcattgct tgaaagatca gcaatatatt cttttgtacc cttttttacg
    11761 gtatcctgag caatactgct atctgaaatt attgccagtg agaaaacaat acataatgtt
    11821 aacatcatta tttttttcat ctttcacctc acaaattttg accaaataat ccaatgaata
    11881 acactttgaa acaattatag cttatattta gaaagcttgt caatgatttt attaaaatta
    11941 ttcaccataa attcattttt tcaaaattat acatggttac agtcattatt tagcccattg
    12001 tatcttcatt ataaagtgct ttacatgata ttcccatgtt ttgcttcaat gctatgataa
    12061 tatgcttttt agctcttgtt aaaaaaaatt acatttcttg atttaaatag aatataatat
    12121 taaaaacttt gtgaatcata cgggaaacca tcataattag atttttgaaa gttcttcatg
    12181 ggtacaagta ttataatgat gttgcatcat attgtttata gttaaaaaac atttttagag
    12241 atgatcatac gttaaaataa taattgaaat attatgcata gtttgttaca tattattcaa
    12301 tgcaccatta ggggaatgtt atcatcatga acacaatatt aatagtagat cataccgatt
    12361 acaaaaacat actaaaagat tatttttcgt catactattc ggtgcttttt gctgattgtg
    12421 catttgacgc tatttcacta ttaaatatgc acgacattga tctggttatt tcacaggttg
    12481 ctttgcctgg cgataatgct tttgatcttt ataactatct ccaaaatcat tatacctata
    12541 tacctgctat aatgataacc gaaagcaata tagatatttt ttttgatgaa atattcagac
    12601 agggaatagg aaatgtttta aaaattccgg tgaataaaac tgaatttgtg caactggtcg
    12661 ataaaataat atcacggaac aatgtatttg gattgtcaaa ttatattcat aatataattg
    12721 aaactaattc catccgcatt aacgcttcaa aacaaattca atcgggagtt gatactatac
    12781 ttgcaaccat tgaatcatgg ggatttacca ttaaaaataa agtagttcta agtttaatac
    12841 taaatgaaat gattataaat gccgtttacc attcacatgg atatacacat gaaaaagaaa
    12901 tgcgagttcc tattgaactg aaagaagggc aatacgttga tatttcattt tgtcataatg
    12961 cctccacata tggtatcgca gtcacggatt atcaggggac gctatcaaaa ggaaaaatac
    13021 tggaaagcat ccataaagcc attgaacagg aacagcttat tttgcatgca gctaaaacag
    13081 gcgaagatat aaccgataaa atttctgaga caggacgcgg cattgatctt gtgcgaaaat
    13141 tagcatctga attttatttt gttattcaga aaaataaacg tactgagatc atattgcttt
    13201 ttgagtcatc atcctcaata caaacatcaa gctcattaaa aattatcgaa gatatgcgat
    13261 agtttcttac ttttattctg catttcttcg taaaaacgcc ggtatatcaa gatcctcatg
    13321 cgagtaatct ttaacccttc gtttttcata gtccagcgta aatgacttca tctgagcagg
    13381 aacctttttt tcaatccgct ccttatgatt cttcccttca atcactgtca atggcgtccc
    13441 cgttgctcta tccagctcat taggtctgtt cagcatattg agtttacggt caaaaccagt
    13501 tgctatcacc gttacccgta tcttatcatc taaagcaggg tcgatagttg ctccaaaaat
    13561 tatgtttgca tcaggatcaa cctgtttgtt aataatttct gttatctcgt ttacctcatg
    13621 taatgacata tcactgccac cagcaatgtt gagcaatacc gcttttgcac cttcaatacc
    13681 tgattcttca agcaatggac tgttaattgc catctgtgtt gcttctatcg ctctattttc
    13741 tccaactcca ataccaacac ccattattgc atcacctgtt tctttcatta cagtacgcac
    13801 atcagcaaaa tctacattga ccaatccggt taccatgatt atatcagcaa taccctgcac
    13861 accctggcgt aaaatatcat ctgctaattt aaacgcatga tccattggcg tactacgatc
    13921 aattatcttc aataacaggt cgttaggtat tacaatgatg gtatcaactt tttccttaag
    13981 atttttaata ccttcgtcag catgttgcat tcttctcctg ccttcagctc tgaatggttt
    14041 ggtaaccact ccaacaacaa gcgctccact ttctttagcc acctctgcaa ctataggtgc
    14101 agcacctgtt cccgtcccac cacccatccc ggcagtaatg aacaccatat cagctccctt
    14161 cagagctttt tccagcttat ccctgtcttc caatgctgct tccctgccta ttgatgggtc
    14221 cgcacctgca cctaaacctc ttgttaactt tgttccaatc tgaatttttg tctgagccaa
    14281 tgatgcttta agctgttgcg agtcagtatt aacaacaata taatcaactc cttctaaacc
    14341 tgttgctatc attctgttga ctgcattgtt gccggctcct ccaactccaa ctactttaat
    14401 aatagtattc atctgtggtt cttcatcaaa tttaaacatt attgcctcct tttatattat
    14461 gtcgcattat tctttaaaaa aatttataaa aaaattactg taatgctcat attaaagata
    14521 atcattaaaa atttgacgca atttatcctt aagacttttt aatcctccat gagtttttct
    14581 ggtttgccgt aattgcccca ttttagcgcc atatcgcaac aatccaactc catttgcata
    14641 catcggggtt gatacaacat ctttaagacc aacgatgttc tccggtaagc caaccctgac
    14701 aggcatattc aatactcttt cggctgcatc cacagttcct tcaatcatgc tgcctccgcc
    14761 ggtcaggaca acacctgcag ccaatatttc ctttttgccg cttttgacca attcgttatc
    14821 aatcatctcc attatttcta ttactctggg ttcaataatc tgtgccagtt catgtctgaa
    14881 caatcggcgt ggggctctcc cgccaactga tggaacctca attgtttcag atgcatcaac
    14941 cagatcaaca accgcacatc catatttctt cttaataacc tcggcagaat caatgggagt
    15001 acgtaaacct attgatatat cgttggttac atggatgcct ccaatcccca atactgctga
    15061 atatgccacc ccaccttcta taaaaaccat tatatcggtt gtgccacccc caatatctac
    15121 cagagctact cctaaatctt tttcatctct actcaatgta gactcggctg aagctaacgg
    15181 tgaaaacact atatcattgc acacaaaacc tgctttatta actgacttaa ctaaattctg
    15241 aatgcttgtt gtagaacctg ttataatatg gacctctgcc tccagccgta ccccgctcat
    15301 acctatagga tctttaatac ctgtttgatc atcaacagca aactctttgg acaatacatg
    15361 gataatttcc ctgtccatgg ggataacaat tgcctgtgct gcctcaataa cccgtttcat
    15421 ctcaacaggt gttatggttc tgttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnatgttt tccataatgg tatcttcttt catagtttta ccacaccact atcctttttt
    15601 gtgtaaccac gccattgccg ggacaatgag atttactatc aagcaatcca ggaaccgaat
    15661 acattagtga aatcccttca tatgtttcat cccatatata acgtgtttgt aaaccatgaa
    15721 ttgaaacata cgagacaaat ggattaatac aatcaataac ggatattcta cctatagcct
    15781 gtccctgaac attcttaagt ttattcataa tcataagaat ttttataatt cttggcgata
    15841 gtttattatg ataaacctca tcctgtgtta tttttattat cggcctgtcg tatgcatgaa
    15901 ctctataggt tgaaattatt tttagatgac tatcgacctc tattggtata gtatcattgc
    15961 ctttttcaat aagcacaata gcaaggatat ccttttcttt tactttaaca gttaaaatgt
    16021 tattgtttgt atccacagta tacgactcta tcataggatt gttttgtaat gaattattca
    16081 gcttcttcat atcaacaaaa aacctatcat ctttgctaat cacggcatta tttaatattt
    16141 caaactttgt tacatatttt aatccgtaaa gcttaacagt gctaattgct tctaccgatg
    16201 cataaccata taagggaaat agtaaaaggc tcacaatcat actggcagtg tataagttac
    16261 ttttcactgt accacaccct ttttatctaa cattgaaagt atggcatgcc caaccttata
    16321 gatatcgcct gcacctaatg ttactatgac atcaccttcc ctgactattg gacttatgta
    16381 gtccggcacc tgatcaagat gaggtatcac aacaacatcc ctgccttcat gctttttaac
    16441 agattgttgt attaaattgc tgcttacccc ttcaatgggc tcttcaccag caggatagat
    16501 ttcagttaaa aaaagctcat cggccattgt aaaagcctga ccaaattcat catataaata
    16561 ttgagttcgt gtataccgat gtggctgaaa caccactatt accctttttc ctaattgttt
    16621 taacgcatcc agggtagccc gtatctccgt tgggtgatgg ccataatcat cgacaacgat
    16681 aatcccattt gtttcaccaa tcttttccag acgtcttcca acaccttgaa actgagctat
    16741 agcttcagct ataacaccaa acgttaatcc caattcaaga gcaacagcca caacagccag
    16801 tgaattaata atattatgct tccctaataa attaagaact atctccccta ataatttatc
    16861 attataataa caggaatacc ttgttgatcc attcaacaac ttaatattgg tagctttaaa
    16921 atgagctccg gtattaaatc cataggtaac atagggacgc tcaatcctgg gcaatacctc
    16981 agctacaacc ggatcatcca tgcaaactac tgaatatcca taaaacggga tattattcat
    17041 gtacgtaatg aatgcttctt taatgccctc aaaatattta taatgatcca gatgatctgc
    17101 atcgatattg gttacaatcc caatagtagg taaaagcttt agaaatgacc cgtccgattc
    17161 atccgcctca aaaacaaaat attgcccttc acctattctt gcattgctgc caaaatttaa
    17221 aaccttgcca ccaacaacgg cagtaggatt taatcctcca tgatataaaa tataggctaa
    17281 catggatgag gtggttgttt tcccatgtgt acctgcaaca cctattccat gttttaatct
    17341 caccaattca gcaagcattt ctgacctgtg tattaccggt atttttcttt tctttgcctc
    17401 tattatttca ggattatttt tactaatagc gcttgatgtt acaaccacat ggtaatcctt
    17461 tatgtttgat ggataatggc caatgtaaat ggttgcaccc aattggcgca accgtttggt
    17521 ttgctctgat tccctcaaat ctgaacctga cacatcataa cccaaattga ttaaaatctc
    17581 ggcaatacct gacatcccga tgccaccaat gccaataaaa tgcatttttt tggattttct
    17641 aaacactact actcgctcct attttttcaa atcagaatct ttaataatat aattaataat
    17701 aatttctgcc gcattatgct ttgcaatgga ctttgcttta gcactcattt tccgtaataa
    17761 cgtttcatta tcaagtaaat caaacaatag cgtggcaact acttgtgcag ttgcgtcact
    17821 gttattgatc atttctgcag cacctgcttt cacaaactcc tgtgcatttt ttttctgatg
    17881 atcctttgct gcaaaaggat atggaatcaa aattgaagga ataccccatg ctgcaagctc
    17941 catcatgaca cctgctccgg cacggctaat agcaatatca catgctgcat atgaattttc
    18001 aacatcagta atatatggtg caagataaat tgatgcatgc tttatggtat gcagcttttc
    18061 tttaaactgc tggtatgaat atgtaccggt actccatata atacctacat ctgcaaatct
    18121 atccggatag ctaacaagca tatcatatgt aagttcatta agcttcaatg caccctgact
    18181 gccccctatc accaatatta cctttttaca gtggatcata ttaaaacgtt ttaaggctgc
    18241 atctttttct acaggtgtta acacctgttt ccgtatgggg ttgccagcat gtacaatttt
    18301 accagttgat tccacatcaa aatatgttgt agtggcttca aatgtagtaa atattgctct
    18361 tgcacgcttt gcaaaaaaat tggttaccat accgggcact acattttgtt cacatagata
    18421 tataggtatc ttctttatca ttgctgcaac aagcgatggt gctgaaacat atcctcccat
    18481 accaataact gctgctatat tattttttgc tataaacttc attgtccata gcattgccga
    18541 aataaaacgt attgtaaaca ctggaaaatt aacaatattt ttccacattg ttggcgcatt
    18601 atacacctgg atattttcca cactattcag ggatgaaaat ttcacatcac cggtaccaac
    18661 cagcaggaat acctcatatc ctttgctttt caactcttca tacaacgcta tccccggcat
    18721 tatatgcccg cctgtcccgc caccacttat caatactggt gttttcatac cagcacctct
    18781 ttatcaaatt taatatccct tggaacaacc tcgcgatatt ttgaaatgtt caacaatatt
    18841 cctgttgcca tcatattaac caataacgaa gaaccgccat aacttataaa cggaagcggt
    18901 atgccagttg ttggaagcag cccaatgaca acactgatat tgagaaatgc ctgaactgtt
    18961 ataagaagtg ttaaccccat tgctagtagt ctcccatatt catccggtgc tttcagtgca
    19021 attttaaccc caaaatagaa aaaaaataaa taaagaataa gaatgctgaa tgttcccaat
    19081 aaacctgctt cttcggcaat gactgcatat ataaagtcgg tatgtggttc cggtaatcgc
    19141 ttaatcttct gagttcccga acccaatccc gcacctaaca tacctcctaa tttaaatgca
    19201 ataaatgatt ggataatatg atatccctta ccaaacctat ccgaccatgg atcaaaaaaa
    19261 gctaaaatgc gctgacgtct atactcaacc tgatatatta acagataaaa cattggcaca
    19321 ctcaataatg ccagacctat catatatatc aatggaaaac cggaaacaaa aagtaacact
    19381 accgaaatga tggccagatt aatagctgtt ccaaaatcag gctgcaacat aataagcaaa
    19441 aagaaaatac ccactaccag caatggcaac aaaagctgta aaacattaat ttcccgatga
    19501 ttgtcatatg aaaatacttt tgcaaaatat ataacaacaa ataatttcac taattccgct
    19561 ggctggaatt gcaaaggacc atatcccagc cagcgagatg aacctttaac ggtaatacct
    19621 acgccgggta ttaataccag aataagcgca atcatggtaa acaacagcat tatacgggtg
    19681 taatgctggt aatatctgta atcaatatgc tgtactatca tcattataaa aaatcccaga
    19741 caacaccata caagctgccg ttttaaaaaa tataacgaat caccaaacac ccgttcagca
    19801 aaaattgcac tggcgctgta actcattgca atccctatgc caataagaat aaacgacaca
    19861 ataaacagtg ctatttctgg ctcgcctttt ctgcgagtgt ctatcaccgc ctttacatcc
    19921 acgccagttc tcctcgcacc aatttagaat aaatttcaat aaagtgattc cctctttgtt
    19981 cataattttt atacatatca aacgaggcgc aggctggaga aagaagtatt gtatcacctt
    20041 tcgcactgag tttcatagcc tttattaaag catcctccat tgaaaatgct ttaatatgcg
    20101 gtatatcgct gaagattgag gcaaattcat cttttgtttc accaatgagc actaacgctt
    20161 tcaccttacc ttttatgact ggtttcaatc gtgaataatc atcgcctttt gccctgccac
    20221 caaggataag tattacatta tcctgcaaac tttttaatgc cattattacc gcaccagtgg
    20281 ttgttgcctt tgaatcatta ataaaaatcc ttcccttata ttcgccaatg ttttgtaccc
    20341 tgtgcggcaa tcctttaaaa ctgtataacg cttcttccag aacttttaaa tcaatattgt
    20401 taccaaactt tttccctata aaggttgatg ccagaatagc cgccatagca ttctgcacat
    20461 tatgcagccc tgctatcttc atgttactgg catatgcaac aagcgttgtt tcaccattac
    20521 tatccataac aattgcacca ttgctgtaaa aacaatctgc tttccccaat aaggaaaatg
    20581 aatgttgttg cgattgtagt gcaggcatgt tgttaactat aaattcatca tccctgttaa
    20641 aaataaaata atcatccttg cactgattaa tggcaatatt catcttagcg ttaaagtaat
    20701 catataatga attgtatcta tctaaatgat caggagtaag attggttatg attgctacat
    20761 caggtttaaa tgttttaacc gtctcaagct gaaatgacga cagctcaatc actgccactg
    20821 aatcttcatg taatgaatcg acaagcgaaa taaacggtat gccaatatta cctcccggat
    20881 gagcgtcaaa gcccagctgc gtaagacaat gagcaatcaa catggttacc gttgatttcc
    20941 catcggtacc ggtaacccct actatggcag ctttacaata gcggtatgct aattctatct
    21001 cggatattac ggg
//
LOCUS       DAOSOX010000022        20885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2543_length_20885_cov_8.933605, whole genome shotgun sequence.
ACCESSION   DAOSOX010000022 DAOSOX010000000
VERSION     DAOSOX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20885
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2543_length_20885_cov_8.933605"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PLX22_03045"
     CDS             <1..1249
                     /locus_tag="PLX22_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT18913.1"
                     /translation="VFYIQDSLPQCLVYVSMGAGSLYEDASNAGISQMLALVLSLGGS
                     KKYPDINQYMDSIGGRYAVNASFEDITITMRVHAGEAETMIAMLTDLIVQPDINDKWL
                     AMARSLLLEQIKQKQDNPMELAFEKARQCVFGNTGYGSVMTPESLAAINTTSLMDYWK
                     RIIAKKNVRIGIVTQRRYDDVKSGVSGLTGAFSDGDSYDYSVDIPTAVKNTSSKTIYF
                     IEKDIPQSTIVMATIAPKIKDPYRYALEVGNYIFGGGSFNSRLMNEIRVKRGYAYATG
                     SIMRFRKNTGLFLAYAQTQAATTLTTVELMETLFHEFGSSGPVDEEVQWAKDAMIKSY
                     IFTFDSLRSIAGYYLWMDYHGLPGTYINEYPDALAKVSREQISHAWVNAMKGYIIVIV
                     GNKQVKEHIENDLEKNRVVIINQ"
     gene            complement(1239..2066)
                     /locus_tag="PLX22_03050"
     CDS             complement(1239..2066)
                     /locus_tag="PLX22_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18914.1"
                     /translation="MKTKFIRIDDVSEIDTSKIGIFDMANRYIDKYGNMYGLRYNRDV
                     KKIEVVKIMRTQKKHATYFSQKIQMRPEKPEAPHNDDYLGEEMEFDPEACINHIVELS
                     KTHKNRLSGIIMNIANSNIIADTDKMLWNTMNDLFRNIDMDAIQQLEKIADLYNEFNN
                     YPRSIIYYQSKLDTKHRKILDEIPAESSKFRYVKYYEMWYITKTTYKTIIKLLNDIRF
                     LLEQQHIEDKRNLTPFQKQTCSDALTSIDNTITEANTILADCGMVEELINTSKAFIG"
     gene            complement(2080..2457)
                     /locus_tag="PLX22_03055"
     CDS             complement(2080..2457)
                     /locus_tag="PLX22_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT18915.1"
                     /translation="MTEKRIHPRKECYIKTKFDYFEGDPETVSVNQKPAKSSKGYILN
                     VSISGAFLVCNCRVSVQMPIKLYCKIQNTKLELEGEIVRTGMVQNNPSEVAQRYAAKN
                     VKGDSYIAIRFKELLPQSIIEQV"
     gene            2829..4454
                     /locus_tag="PLX22_03060"
     CDS             2829..4454
                     /locus_tag="PLX22_03060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT18916.1"
                     /translation="MKDKKTKFIFVTGGVCSSLGKGISTASLGMLLEGHGYKLSIIKM
                     DPYINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERLTNAELTRKNSVSTGQIYYEV
                     ITRERRGDYLGRTVQVIPHITNEIKKRIYDVANMDDLDFLLVEIGGTVGDIESIPFLE
                     SIRQIRQELGKSRVLNVHLTLVPTITVAGEQKTKPTQHSVKELMELGILPDILLCRSA
                     RTLSDELKSKIALFCNVSERYVISAVDISHSIYEIPYMLHENQYDEVVLEHFGLPVKP
                     LHIPAWDTFFTSLMNPKKKVTIAVVGKYISLQDSYRSIYEALIHGAVANNAELEIQRI
                     ESEEIERKYMNSLEELFKNVNGILIPGGFGTRGIEGKIATIEYARTHKIPLFGICLGL
                     QCAVIEFARHGCGMENANSTEFDPTTPYPVISLLEEQELIDKLGGTMRLGAYLCMFKD
                     NTRIKSIYNTDSVMERHRHRFEFTLKYKQLFEKHGMIFSGFNPQSNLVETIEYADHPW
                     FICTQFHPEFKSKPVKPHPLFKDFIRASMENIC"
     gene            4576..6009
                     /gene="rpoN"
                     /locus_tag="PLX22_03065"
     CDS             4576..6009
                     /gene="rpoN"
                     /locus_tag="PLX22_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOT18917.1"
                     /translation="MALLKLDVVQTQRLRLTQTLRQSLDLLQISTLELEEILLQELQE
                     NPVLECDDALNEGFESLEDVAKNEMEGTVASKAEEFSIEYADASDTGIQNDFDKKNVL
                     EIVTRNESLQEHLLSQIRMLSLPQEDIDILEQLITSLDDNGFLTVSKEFIQNNYNIAS
                     EKLDSLLAVLNTMEPVGCGVFNVTESLMVQAQYYYPEDDLLQVIIKNYLPNIQRLEYD
                     RIAKNLNIRREEVKEKAILLTNLTPFPGALYLNSKPIYVRPDIEVQVIDDEVVIQLVD
                     NWIPRLYINAKYVQMLKQKKVDEKTRQFIHEKVSSAKNLITNIGRRNETLLLVAYAIM
                     NRQKEFLQKGQGFLKPLIYADLESELGFHESTIARAVANKYAQTKWGVFEFKYFFVKK
                     VKSEHNDETSQDTILKRIKELINSELKHNPLSDEEILDILRGEGIHIARRTIAKYRDM
                     LSIPASHIRKKLYLIKDEGNHEFNNHR"
     gene            5987..6796
                     /gene="raiA"
                     /locus_tag="PLX22_03070"
     CDS             5987..6796
                     /gene="raiA"
                     /locus_tag="PLX22_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT18918.1"
                     /translation="MNLTITGRNFEVSDSIREYAEKKLTKLTKYFHQLIDMHIRLFIE
                     RQDHVAEITINGDGVQFYAKEKAGDMYSSIDLLLDKIEQQIVRYKERHSEHKVTPLNK
                     LEDIATYNVSGEQTPIVIRQVSNKPIDEKEAYLQMKLEGAEFMLFKKGEKSIKNAVDY
                     MNKNYAVIYKDGDTYTLIELPFEMIQNNNFDPSKFNVYSLDVKKESLINPEIKFIKKN
                     SCSVKIMSVDDAVVEITKSDVPFMVFFNSETHFLNIIYKKGKSLELLVPAF"
     gene            6806..7777
                     /gene="hprK"
                     /locus_tag="PLX22_03075"
     CDS             6806..7777
                     /gene="hprK"
                     /locus_tag="PLX22_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOT18919.1"
                     /translation="MKKYLRIHDILENTQKIDLQLTLVAGSEGLDKAITAIEINRPGL
                     TLAGFFDYFAYDRIQIFGLGETAYIKTLSRERREEIYHKFFSYDIVSCVYTHNEYPDE
                     IFIDFAKKYKVPTFVTRYPTTRFISLLTHIVEAAFAESVTIHGTLVDVFGIGILLIGK
                     SGVGKSETALELIERGHRLIADDLVEVKKIDESLLMGNGSELIRHHMEIRGIGILNVR
                     DIFGIRSVRNRKRIELVAELQEWDANTHYDRLGIEEQLYTILDLEVPYIIVPVRPGRN
                     IPIIIETAALNQRLKKIGIFSAQELDKKIQEWIVRENEDYEREKGEN"
     gene            7752..8027
                     /locus_tag="PLX22_03080"
     CDS             7752..8027
                     /locus_tag="PLX22_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT18920.1"
                     /translation="MKEKKVKIKSDAGVHARPASILVREAMKFPCDIYLIKGQMEANG
                     KSIMSVLGLAITKGSFLTIRADGEMEEEAVNRLSALIENDFAELASK"
     gene            8024..8599
                     /locus_tag="PLX22_03085"
     CDS             8024..8599
                     /locus_tag="PLX22_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18921.1"
                     /translation="MKHCCSLSQYAIWILIAIMLLLPVNSFAQSKHKQELSLTDEEIK
                     TLFEKKKDPVFSGILSFYMPGLGQFYCDEKLKGAAFLITEYTIIIGSMFYFLDFNFKA
                     GGDSGFNLKVDANQTDMGLFSIERKHLFYVTVAALSLIHIFNIIDAVKSAYSFNSTLE
                     QKRKNLIQKYPQLDMGCNNDGGMYLAFSSHL"
     gene            8616..9143
                     /locus_tag="PLX22_03090"
     CDS             8616..9143
                     /locus_tag="PLX22_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18922.1"
                     /translation="MVLLCSTVVFAQNGKNVYKPLPLQEVPADVPIFFEEPERKDPFI
                     AGVLSWSWSGLGQFYTQNYKKGSAFLVADIAEKSLLVFMLFHISDKYSHGSEDVNWSD
                     IDKKDQYMVVGLLFSIFFTRVFSVVDAVNSAHDYNKNIYYPYWKSKQSFKTSFENTSS
                     NKSICLMLSHPVNFQ"
     gene            9140..9706
                     /locus_tag="PLX22_03095"
     CDS             9140..9706
                     /locus_tag="PLX22_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18923.1"
                     /translation="MKQLLCIITIVIVLMCSHTLFAQNVVYNDVRFDSTKLIVTISIT
                     DVKTYDILEALKRGLEGRIEYVVEIVENPVLPLLPKNVIKTSTVKKKVKFDFSKKAYI
                     VSRSNVPTLYYSDESIIEELFNNQQIVIDNAYTLRKSNYSIRIRVSFSSVKLYFPLNL
                     IFNYVVGIWDFDTGWEYGPKLVGIPYAE"
     assembly_gap    9719..9818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9819..11423
                     /locus_tag="PLX22_03100"
     CDS             <9819..11423
                     /locus_tag="PLX22_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT18924.1"
                     /translation="IPLAAAVFFIVLAFRLRVHPDGLYGGGSIRAKIALALIVIAIVP
                     TIPIIVITSNILNTTISQFFTEKTMIALERSVDMANEQILSIDNGLIRQLEQLRYDLD
                     SNFVSVANITNASFQKRYTFSQSYLYVTTIEHKGILNNRIQSLFLYPDRERVENDINT
                     FLEIAKVTNAIACSRLAIDDQMYSVAYTQYGQYCIVMYRLIPRDLLSTSEFINTSFSE
                     YKKQEFLKPYFQTGIGIALIMISIGIILLAIVLSLILSQSITKPVYELVEAAEKIAKG
                     NFNINLVRREQDEMALLYESFNQMAKQLDQGKKMMYQTQKLKAWSDIARKLIHEIKNP
                     LTPIRLSAERLSRRYEEHHPDFSSILKEATNTIIEEVTILMNMLSEFSKFARLPEINL
                     QAESINEIIENCIAMYYDNDKISFVLDLKPDIPPVMCDKTLLRQAFLNIIQNAIEANG
                     HVITVSTAYDSQQGALTIAFSDDGVGIPDDEIEKVFEPTFSTKENGMGLGLAIVEKII
                     IDHKGTITCSSTLKQGTKFTITIPQQ"
     gene            11434..12789
                     /locus_tag="PLX22_03105"
     CDS             11434..12789
                     /locus_tag="PLX22_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT18925.1"
                     /translation="MAKILVVDDEKNILNTMKAILQDEGHVVYTVQNGKDALQFVKSN
                     ECDVVFLDVWLPDIDGMEVLQQIKQLQNDIAVIMISGHGSIDIAVKSTRLGAFDFLEK
                     PPSMERVITSLNNAIEQIQLKRENVILKKNITMEDEMIGNSIPMQEVKRIIDTAAATN
                     ARVFITGENGTGKELVARAIYRKSKRSDKPFIKVNCAAIPDELIESELFGHEKGSFTG
                     ALARRLGKFELADKGTIFLDEICDMSQSAQAKVLRVLQEQQFERVGGNDVITVDVRVI
                     AATNVDVKEAIEQGRFREDLYYRLNVIPIYVPPLSERKDDIPLLVDYFLEKFAREHGL
                     GVKQMSDSAMHFMVNYAWPGNVRELKNVIERLTIMVPSEVIELDDIKKHIESYDYEDT
                     IVKDSSSLKKAREQFEKEYIIKALKQFDKNVSATAKSLGIERTNLHRKIRQYHINVDK
                     L"
     gene            12793..13431
                     /locus_tag="PLX22_03110"
     CDS             12793..13431
                     /locus_tag="PLX22_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOT18926.1"
                     /translation="MYSTLKEVLQTYRNYAHRLPMVIAYEDEVIPLTVNKAYHDTAQD
                     VQDTTATTVHEEIERCNQCQSTHKIAMQSRLTEAAIMIVLNPPALISSVEKKVLQPQV
                     DTMLQRMMKAIDVQMENCYITHMIKCESQKQLPGTMFLHCQQFLVKEINHVNPRVIIV
                     MGEMLPMRKIIKSMDAVQWFGIEHPITLIKNPELKKDAWKTLKLVRATLDEK"
     gene            13421..15373
                     /gene="priA"
                     /locus_tag="PLX22_03115"
     CDS             13421..15373
                     /gene="priA"
                     /locus_tag="PLX22_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT18927.1"
                     /translation="MKNSDMFADVAVGLPVEGLFTYSTAEFPITKGMRVVVDFNNRKI
                     IGCVVNVHSQKPDFEIKPVAKIIDDEPIFDERLLAMAQFIAHHYVCYFGEAVSTTMPS
                     GKSYKTRQKTFFAGDSYSEITLTPEQEDIQQAILQQGQKAHCIYGITGSGKTEVYIAL
                     AREMIRRNQSVLYLVPEIGISSQMFMRLKEVFGNSLVMYHSGMSPNERLLSWRRFYHG
                     DAAIAVGTRSAIFMQAPHVGLIIIDEEHDASYKENSTPRYHARTVAWYRHKNEGSMLV
                     LGSATPSLETLYAVQGKRIMMHTLHRRFGNATLPQLEILPVKASGGNKIITNQLLMAI
                     KKTIEAKRQVILLLNRRGFAPRVICDDCGMSVQCPHCSISMTYHKKKLLCHYCHYEST
                     IPEQCEHCGSKNLIYVGSGTQRIEELLTRMFPLATIVRMDQDTARKKNFVAEVVSNMQ
                     QGKIDILLGTQMVAKGFDFPNVALVGVIMADIGLSLPDFRAAERIFALLVQVAGRSGR
                     GDIAGKVIVQTINPDNPLFNHLKNYDYIGFCQRELSYRKALNYPPYVRLARLVIRGKD
                     EDEVAQVATLYKKKLDELLAHYPGITILGPSQAPLYKIASYFRYHIVLKSGSVDALSG
                     MIAQCKKNMDVHNAYIEVDIDPLDLM"
     gene            complement(15378..15875)
                     /locus_tag="PLX22_03120"
     CDS             complement(15378..15875)
                     /locus_tag="PLX22_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HOT18928.1"
                     /translation="MPEFPEIPDEIKKLFEEHAIDEIRLDAEGNWFHNGEPFTNQRII
                     RFFNSSITITRDGTYVLHYANFTYPIVVEDAPIFVTGARFEGFADFEHVILTLSSGEE
                     EELSIDTLHIRKAAELYCYVRNNTMLARFKRSAAFTILERLEESDDIFYVTIAGNKIV
                     LEEKK"
     gene            complement(16135..18201)
                     /locus_tag="PLX22_03125"
     CDS             complement(16135..18201)
                     /locus_tag="PLX22_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT18929.1"
                     /translation="MPPVKKNRFTIIPEDSHTQGMRILSIEEENKEEKKSKKLHLSIP
                     KQLHPLIAAMVLIAGVALVVGIVSLVKKPESFLSSIQKSGNNAYKLLPGLSDEKLAPE
                     DISQNQHILRGKTYYFKKNYTSAIDEFNQVVESDASDNDKALALTYMGMIYDDKGDYN
                     KAIETLKRALKYKPDDTTVLKNLALAYRHKKDYPDALATITRAIAIDGNNPDLLLLQG
                     NIYFDMGRFPDAAKSYTKALDIAPDNPQALYNTGLTFLKTGDELSATEYFKKAAAADP
                     TGAIAKSAYSRLGSFYTNRRDYAMAEKYLNLAIQLDPNEPINHYNLGIVYMQRQQFDK
                     ALAEFKAAESSGIYEEELLEGLSQAYASLDRYDEGIAVLERLRPVENRNVAIIARLAE
                     LYYRKGDLDTAFEYYKQITIYEPVSENARVAYANMGNIRDDQHRFDEAVAYYKKALAI
                     DPNDSSTLYNMGIAYKHAGKPELAVAAFNEASRNNPDNPKPRLAIANLYYEKGYLDLA
                     LDEYIKVVRLWPHLQEAQFMLGMLYHSKGRDDYALEAFNRVIAINQNNDYAKKAYVNT
                     GIILSTSKDEKQQAQAIQSLQKALLIAPNDAEALLALGTIAFNQHKYDKAMEIFYQVL
                     EVSNDDAIVAQAYHTIGRCYYAKHDYKKSLQFLTKASELDPSNEQIRLNRKVATEAYE
                     KELSKK"
     gene            complement(18207..18620)
                     /gene="nusB"
                     /locus_tag="PLX22_03130"
     CDS             complement(18207..18620)
                     /gene="nusB"
                     /locus_tag="PLX22_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT18930.1"
                     /translation="MGHRRKAREFAMQALYMYETVGTPVDELTRLDWVDKEIPEDIMD
                     FALNLITGTIANIDCIDNLIKQYSRNWKFERINPVDKSILRISVYALLYCDAIPHAVT
                     IDEGIELGKIYGSENSGQFINGILDAIRIHEGKKR"
     gene            complement(18620..19081)
                     /gene="ribE"
                     /locus_tag="PLX22_03135"
     CDS             complement(18620..19081)
                     /gene="ribE"
                     /locus_tag="PLX22_03135"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOT18931.1"
                     /translation="MNTIEGKLDASGLTIAIIVSRFNEFITSKLLSGALDCLQRHNAD
                     EKNICVVWTPGAFEIPPIAMKLSKGNYDAVVCLGAVIRGATPHFDYVAAEVSKGVAQV
                     SLQSNIPVIYGVLTTDTIEQAVERAGTKSGNKGFDAAMAAIEMAQLYKQLR"
     gene            complement(19326..20090)
                     /locus_tag="PLX22_03140"
     CDS             complement(19326..20090)
                     /locus_tag="PLX22_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT18932.1"
                     /translation="MITKKVEDNVYILTLNDGKTNALTDTVLNDLRKYLKEAASDATI
                     KGVILTGEGKTFSSGFHLPMFLSFTTINEVIKFFDVEEEILLELFMFEKPVIAALNGH
                     TVAGGMIFAMACDYRIMKNHPKIKMGMSEIKIGLPLSVAQWNVVRFGLDSDKKFRDIM
                     YFGNMFSPDEALKMGLVDELADEDKLISRAKELINLWKNNPGNAFTPLKLCVRQETAD
                     RIRKDLTDGSWTQGLQCFFKDDVRKTLEFVQATMSK"
BASE COUNT         6422 a         3659 c         4277 g         6427 t
ORIGIN      
        1 ggtattttat attcaggata gtctgccgca atgtctggta tatgtttcta tgggtgctgg
       61 ttcattatac gaggatgctt ccaatgccgg tataagtcag atgctggcgc ttgttttgtc
      121 attgggcggt tcaaaaaaat atcccgacat aaaccagtat atggattcca ttggcggacg
      181 ctatgcggtt aatgcatcat ttgaggatat aacaataacc atgcgcgttc atgccggtga
      241 ggctgaaaca atgattgcca tgcttaccga tttaattgtg cagcctgata ttaatgataa
      301 atggcttgcc atggcacgat cacttcttct tgagcagatt aagcaaaagc aggacaaccc
      361 aatggaatta gcgtttgaaa aagcacgcca gtgtgtattt ggcaataccg ggtatggttc
      421 agtaatgacg cctgagtcgc ttgctgccat aaatacaaca tcccttatgg attactggaa
      481 acgcattata gcaaaaaaaa atgttcgtat tggtattgtc acacagcgtc gctatgatga
      541 tgtaaaaagt ggtgtaagcg gtcttacagg tgctttttct gacggcgata gttatgatta
      601 tagtgtcgat atcccgacag cagtaaaaaa tacaagcagc aaaacaatct atttcattga
      661 aaaggatata ccacaatcaa ctattgttat ggctactatc gcccccaaaa taaaagatcc
      721 ttataggtat gctcttgagg tgggtaatta catttttggt ggaggatcgt ttaattcgcg
      781 acttatgaac gaaataaggg taaagcgtgg atatgcatat gccaccggtt caatcatgcg
      841 atttaggaaa aatacaggtt tatttctggc atatgcccaa acacaggctg ccacaacgtt
      901 gacaacggtt gaacttatgg aaacattatt tcatgaattt ggcagcagcg gcccggttga
      961 tgaagaggtt cagtgggcta aagatgcaat gattaaaagc tatatcttta catttgattc
     1021 attgcgctca atagctggtt attatttatg gatggattat catggattgc ccggcacata
     1081 tattaatgaa tatcctgatg cacttgctaa agtttcccga gaacagattt cccatgcatg
     1141 ggtaaatgct atgaaagggt atattattgt catagtgggt aacaagcagg taaaagagca
     1201 tattgaaaat gatttagaaa aaaacagggt tgtgattatt aaccaataaa tgctttactg
     1261 gtatttataa gctcttcaac catgccgcag tcagcaagaa tggtattagc ttcggtgata
     1321 gtattatcaa tagatgttaa agcatcagag caggtctgtt tttgaaatgg ggtgagattt
     1381 cttttatctt ctatatgctg ttgttcaagc aaaaatcgta tatcattgag cagtttgatg
     1441 attgttttat atgttgtttt tgttatatac cacatctcat agtacttaac gtaacggaat
     1501 tttgacgatt ctgcaggaat ttcatccagg attttgcgat gttttgtatc taattttgat
     1561 tgatagtaga tgattgagcg aggataatta ttaaattcgt tatacaggtc ggcaatcttt
     1621 tctaactgct gaatggcatc catatctata ttcctgaata aatcgttcat ggtattccat
     1681 aacatcttat cggtatcagc aataatgttt gaatttgcaa tgttcatgat aatgccgcta
     1741 agacggtttt tatgtgtttt actaagttca acgatatggt taatacatgc ttcaggatca
     1801 aactccattt cctcgcccag atagtcatca ttatgagggg cttctggttt ttcaggtcgc
     1861 atttgtattt tttgtgaaaa ataggtagca tgtttttttt gtgtacgcat gatctttact
     1921 acttctatct tttttacatc gcgattatac cgtaatccat acatgttgcc atatttgtcg
     1981 atgtaacgat tggccatatc aaatatacca attttagatg tgtcgatttc ggatacatcg
     2041 tcgatacgga taaatttagt tttcattgta cctgctatgt tatacctgct caatgattga
     2101 ttgaggtaaa agctctttaa agcgtatagc tatataacta tcgcccttaa cattttttgc
     2161 tgcatagcgt tgcgctacct cagaaggatt gttttgcacc atccctgttc gtacaatttc
     2221 gccctctaat tctaattttg tgttttgtat tttacaataa agcttaattg gcatctgtac
     2281 actgacacga caattacaaa ctaaaaaggc tcctgaaata cttacattca gtatataccc
     2341 ttttgaactt ttggctggct tttgatttac gcttactgtt tcaggatctc cctcaaagta
     2401 atcaaatttt gttttaatat agcattcttt tcgtggatga attctttttt cggtcatggt
     2461 attgtcctat tgaataaaaa ttaataaatt ttctttacag aagtttggat tgtaagtata
     2521 gtatctttga atgttttgtc aagaatagtt ttattatacg ctaaaatttg cgatagttac
     2581 tacaaattct tgacatatgc atggcaggtc aaatagtgac ttgaataata cgtgaagatg
     2641 gaactatttg tgacagttat tctggttgag aacgtcatcc tgaacaccga ttcaggatct
     2701 catggtggga aaagagattc cggatcgagt ccggaatgac tgtgatgaga acgtcatttt
     2761 gaattatgtc ctgaactccg attcgggatc tttaatgata attttactac aactgtttta
     2821 gagggcatat gaaggataaa aagacaaaat ttatatttgt aacgggtggt gtgtgttcat
     2881 cactgggtaa gggaatttca actgcatcat tagggatgct gcttgaaggt catggctata
     2941 agctgtccat cattaagatg gatccttata taaatattga tccgggaact atgagtccat
     3001 ttcagcatgg cgaggtgtat gtcactgagg atggtgctga aacggatctt gatctgggct
     3061 attatgaacg gctgaccaat gctgaattga cacgaaaaaa ttctgtatct accggtcaaa
     3121 tatactatga ggttataacg cgcgaacgtc gaggtgatta tttagggaga acagtgcagg
     3181 ttattcctca tatcaccaat gaaataaaaa aacgaatata tgatgtggca aatatggacg
     3241 atctggattt ccttctggtg gagattggcg gcactgtagg agatattgaa tccataccat
     3301 tccttgaatc aatacggcag atacgtcagg aattagggaa atcccgtgta ttgaatgttc
     3361 atttaacact ggtccctact attaccgtag caggcgaaca gaaaacaaag ccaacccagc
     3421 attcagtaaa agagcttatg gaattgggaa tactaccgga tattcttctt tgcaggtctg
     3481 ctcgaacatt aagcgatgaa ttaaaaagca aaattgcatt attctgtaat gtatcggaac
     3541 ggtatgttat ttcagcagtt gacatcagtc actcaatcta tgagatacca tatatgctgc
     3601 atgaaaatca gtatgatgag gttgtactgg aacattttgg tttgcctgtt aaaccactgc
     3661 atatccccgc gtgggatacg ttttttactt cgttgatgaa tcctaagaaa aaggttacga
     3721 ttgctgtagt tggcaagtat atctcgttac aggattcata ccgctctata tatgaagctt
     3781 taatccatgg ggctgtggca aataatgcgg aactggaaat acaaaggatt gagtcggaag
     3841 agatagaacg taaatacatg aattcactgg aagagctttt taaaaatgtt aatggtatat
     3901 taatacctgg tggttttggt accaggggaa ttgaaggtaa aattgccacg attgaatatg
     3961 cccgaacaca taaaataccg ctgtttggta tttgtttagg gttgcaatgt gctgttattg
     4021 aatttgctcg tcatggatgt ggcatggaaa acgcaaattc aactgaattt gatcccacca
     4081 caccttatcc ggttatctcc cttcttgaag aacaggagtt gatcgataaa ttaggcggca
     4141 ccatgcggct gggagcttat ttatgtatgt ttaaagataa taccagaatt aaatcaatct
     4201 ataatactga ttcggttatg gaacgtcacc gccatagatt tgagtttacg ctaaaatata
     4261 aacaactatt tgaaaagcat ggcatgatat tctcagggtt taatcctcaa agcaaccttg
     4321 tagaaactat tgaatatgct gatcatccat ggtttatttg tactcagttt catcctgaat
     4381 ttaaatcaaa acctgtaaag ccgcatccat tgtttaaaga ttttatacgt gcatccatgg
     4441 aaaatatttg ttaaatatgc aatttaaata gacgttgcca atactatttt tgtatattta
     4501 tacgtaggca aagaaataga atatgtgaaa aagaatttaa catttgaatt gaaggcgatt
     4561 agtgcgagga aatgaatggc tttattaaag cttgatgtag tacaaaccca gcgtttacgt
     4621 cttactcaaa ctcttagaca atcattagat ttattacaga tttccactct tgaactggag
     4681 gagatattat tgcaggagtt gcaggaaaat ccagttttag aatgtgatga tgctctcaat
     4741 gaaggatttg aatcgcttga agatgtcgct aaaaatgaaa tggaaggtac tgttgcatca
     4801 aaagctgagg agttctctat tgaatacgct gatgcaagtg ataccggtat acaaaatgat
     4861 tttgataaaa aaaatgtttt ggagatagtt accaggaatg agtcattgca ggaacatctg
     4921 ttatcacaga tacggatgct gtcattgccg caggaagata ttgatatatt ggagcaattg
     4981 attacaagtc tggatgataa tggtttttta acagtttcaa aagagtttat ccagaataat
     5041 tacaatattg cttcagaaaa attagacagt cttcttgctg tactcaatac tatggagccg
     5101 gttgggtgtg gggttttcaa tgttacggaa tcgctcatgg tgcaggcgca gtattactat
     5161 ccagaggatg atctgttgca ggtgattatc aagaattatt taccaaacat acagcgcctt
     5221 gaatatgaca ggatagcaaa aaatttgaat attcgcagag aggaagttaa agaaaaagca
     5281 atattgctga caaatctaac accttttccc ggtgcactct atttaaatag caaacctatc
     5341 tatgttcgtc ctgatataga ggttcaggtg attgatgatg aagtagttat acaactggtg
     5401 gataattgga ttcccagact ttatatcaat gctaagtatg tccagatgtt gaaacaaaaa
     5461 aaagttgatg aaaaaacacg tcaatttata catgaaaagg ttagttcagc aaaaaattta
     5521 atcacgaata ttggaaggcg gaatgaaact ttattgttag ttgcttacgc tattatgaac
     5581 aggcaaaaag agtttttgca aaaggggcag ggttttttaa aaccgttgat ttatgcagat
     5641 ttagaatcag aattgggctt tcatgaatca acaattgccc gtgctgttgc taataaatat
     5701 gctcaaacaa aatggggcgt ttttgaattt aaatattttt ttgtaaagaa ggttaaatct
     5761 gaacataacg atgaaacatc acaggataca atattaaagc gcatcaaaga gctcatcaat
     5821 agtgagctga aacataatcc cttgagtgat gaggaaatac ttgacatctt aaggggtgaa
     5881 ggtattcata ttgctcgtag aactattgca aaatacaggg atatgttgtc tatccctgca
     5941 tcacatattc gtaaaaaatt atatttgatt aaggatgagg gtaaccatga atttaacaat
     6001 cacaggtaga aattttgaag tgtctgatag catacgcgaa tatgctgaga aaaagctaac
     6061 aaaacttaca aagtattttc atcagttgat tgatatgcac atacgcttgt tcattgagcg
     6121 gcaggatcat gtggcagaaa ttacaattaa tggtgatggt gtgcagtttt atgcaaagga
     6181 aaaagcagga gatatgtatt cctccataga tcttttgctt gataagattg agcaacaaat
     6241 tgtgcgatat aaagaacgac attcagaaca taaagtaacc ccattaaaca aattagagga
     6301 tattgctacg tataatgtca gtggtgaaca aacaccaata gttatacgcc aggttagcaa
     6361 taagccaatt gatgaaaaag aagcatattt gcagatgaaa ttagaaggtg ccgaattcat
     6421 gcttttcaag aagggagaaa aatcaataaa gaatgctgtt gattatatga ataaaaacta
     6481 tgctgttatt tataaagatg gagataccta cacattaata gaactccctt ttgagatgat
     6541 acaaaataac aattttgatc catctaaatt caatgtatat tcactggatg ttaagaaaga
     6601 atcattgatc aatcctgaga ttaaatttat aaagaaaaat tcatgctctg ttaaaattat
     6661 gtcagtagat gatgcagttg tagaaataac taaaagtgat gtcccgttca tggtattttt
     6721 taactctgaa acacattttt taaatattat ttataaaaaa ggaaaatcac ttgaattatt
     6781 agtgccagca ttttagaaaa gagaaatgaa aaagtatcta cgcattcatg atatcttaga
     6841 aaatactcag aagatagatc ttcagcttac gcttgttgct ggcagtgaag gtttagataa
     6901 ggctataaca gcgatagaaa ttaaccggcc ggggcttacc ctggctggct tttttgatta
     6961 ttttgcttat gatcgcattc agatatttgg tctgggtgaa actgcatata tcaaaacatt
     7021 atcccgagaa cgacgcgagg agatatacca taaattcttc tcgtatgata tagtcagttg
     7081 tgtatataca cacaatgaat atccggatga aatatttata gattttgcaa aaaaatataa
     7141 agtcccaaca tttgtaacca gatatccaac aacacgattt ataagtttgc taacgcatat
     7201 tgttgaggct gcatttgctg aatcggttac tatccatggt acactggtag atgtctttgg
     7261 aattggtata ctcctaattg gtaaaagtgg tgttggtaaa agcgaaacag cgcttgagct
     7321 cattgaacgt gggcatcgct taattgcaga tgatctggta gaggttaaaa agattgacga
     7381 atcgttgctt atggggaacg gttctgaact gatacgccat catatggaaa tacggggtat
     7441 tggaatttta aatgtgcgtg atatatttgg tatacgatca gtacgaaacc gtaagcgtat
     7501 tgagcttgtt gctgaactgc aggaatggga tgcaaatacc cattatgata gattgggaat
     7561 tgaagagcag ttatatacta ttcttgatct tgaagttccc tatattattg tacctgtaag
     7621 accgggaagg aatataccga taattattga aacggctgcg cttaatcagc ggcttaaaaa
     7681 aatagggata ttttccgctc aggaacttga taaaaagatt caggaatgga ttgtacggga
     7741 gaatgaagat tatgaaagag aaaaaggtga aaattaaaag tgatgctggt gtccatgcgc
     7801 ggcccgcttc aatacttgta cgtgaagcaa tgaagttccc ctgtgatatt tatttaatta
     7861 aaggacaaat ggaagcaaat gggaaatcaa tcatgtctgt gttagggctg gctatcacaa
     7921 agggaagctt cctgacaata cgtgctgatg gtgaaatgga agaagaagca gttaacaggt
     7981 tatcggcatt aattgaaaat gattttgcag agctggcttc aaaatgaaac attgttgctc
     8041 attatcacaa tatgctatct ggattctcat tgcaataatg ctgcttttgc cagtgaatag
     8101 ttttgctcag tcaaaacata aacaggaact atcgcttact gatgaagaaa taaaaacatt
     8161 atttgaaaaa aagaaagacc ctgttttttc aggtatactt tcattttata tgcccgggct
     8221 tggccagttt tattgtgatg aaaagctaaa aggggctgca ttccttatta ctgaatacac
     8281 tattattatt gggtcgatgt tttattttct ggattttaac ttcaaagcag gtggtgattc
     8341 cggatttaac cttaaagttg atgcaaacca aacggatatg ggtttattca gtattgagag
     8401 gaagcattta ttttatgtaa cagttgcagc actatcactg atccatattt ttaatatcat
     8461 agatgccgtg aagagtgctt attcatttaa tagcacactg gagcaaaaac gaaagaacct
     8521 tattcaaaaa tatccacaac ttgatatggg atgtaacaat gatggaggta tgtatcttgc
     8581 tttttcctcg catttatagg ataactacaa cctgtatggt acttctgtgc tctacagtag
     8641 tttttgcaca aaatggaaaa aatgtttata agccattgcc gcttcaggaa gtacccgctg
     8701 atgtgccaat tttttttgaa gagcctgaac ggaaagaccc ttttattgcc ggtgttttat
     8761 catggtcgtg gagcggttta ggacaatttt atactcaaaa ttataagaaa ggttcggcgt
     8821 ttttggttgc cgatattgcc gaaaaaagtt tactggtttt catgttattt catatatctg
     8881 ataaatattc gcatggttcc gaagatgtaa actggtcgga tattgataaa aaagatcaat
     8941 atatggttgt ggggctattg ttttcaatat tttttacaag agtgttttct gtagttgatg
     9001 ctgtaaattc agctcatgac tataataaaa atatttatta tccgtactgg aaaagcaaac
     9061 aaagttttaa gacatccttt gaaaacacca gtagcaataa atctatatgt ttgatgctat
     9121 cacatccagt taatttccaa tgaagcaatt actttgtatt atcaccattg ttattgtgtt
     9181 aatgtgctcg cacacattgt ttgcacagaa tgtggtatac aatgatgtgc gatttgattc
     9241 aactaagctc atagttacca tttcaattac cgatgttaaa acctatgata tactggaagc
     9301 cttaaagcga ggattggaag ggaggattga atatgttgtt gagattgttg aaaatcctgt
     9361 actgcctctt ttacccaaaa atgtaataaa aacaagtacg gttaagaaaa aagttaaatt
     9421 tgatttttct aaaaaggcat atattgtgtc ccgatcaaat gttcccacac tgtattatag
     9481 tgatgaatca attattgaag aacttttcaa taatcagcag atagttattg acaatgccta
     9541 taccttacgg aaatcaaact attctatacg tattcgtgtt tcatttagtt cggtaaaact
     9601 atattttccc cttaacctga tatttaatta tgttgtaggt atatgggatt ttgatacagg
     9661 ctgggaatat ggtccaaaac ttgttggcat accttacgct gaataacggt atggtatgnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat accgctggct gctgctgtat
     9841 tttttattgt gctggcattc aggctcaggg tgcatcctga tggtctttac ggtggcggca
     9901 gtatacgagc aaagatagca ttagctctta ttgttatagc gatagtccca acgataccaa
     9961 tcatcgttat taccagcaac attttgaaca ctaccatcag tcagtttttt acagaaaaaa
    10021 caatgatagc ccttgaacga tctgttgata tggcaaatga gcaaatactg tcgattgaca
    10081 atggtttaat acgacaatta gaacaactca ggtacgatct tgatagtaat tttgtaagtg
    10141 tggctaatat taccaatgct tcatttcaaa aacggtatac attttcgcaa tcctatctat
    10201 atgtaacaac tatcgaacat aaaggtattt tgaacaaccg catacaatca ttgtttctat
    10261 acccagacag ggaacgcgtt gaaaatgaca ttaatacttt tctggaaata gcaaaggtaa
    10321 caaacgcaat agcatgcagc agattggcaa ttgatgatca aatgtattca gtggcatata
    10381 cacaatatgg tcagtattgc attgtaatgt atcgattgat accaagggat ttactttcta
    10441 cttctgagtt tatcaatact tcatttagtg aatataaaaa gcaggaattt ttaaaaccgt
    10501 atttccaaac cggcattgga attgccctta tcatgatatc aattgggatt atattgcttg
    10561 cgatagttct tagtttaata ctatctcaga gcattacaaa gcctgtatat gagctggttg
    10621 aagccgctga aaagattgca aagggtaatt ttaacataaa tctggtacgc agggaacagg
    10681 atgagatggc attgctctac gaatcattta accagatggc aaaacaactg gatcagggta
    10741 agaagatgat gtatcaaaca cagaaattga aggcatggag cgatatagca cgaaagctca
    10801 tccacgaaat taaaaatccc ctgacaccta tacggttatc ggcagagcgg ttgtcccgac
    10861 ggtatgaaga acatcatcca gatttttcct ccatccttaa agaagcaaca aatacaatta
    10921 tagaggaagt aacaatactt atgaatatgc tttcggagtt ttcaaaattt gcaaggcttc
    10981 cggagataaa cctgcaagcg gagagtatta acgagattat tgaaaattgt attgcgatgt
    11041 attacgataa tgataaaatt tcatttgtgc ttgacttaaa acctgatata ccacctgtta
    11101 tgtgtgataa aacattatta cggcaggcat tcttaaatat aattcagaat gctattgaag
    11161 caaatggaca tgtaataaca gtatctactg cttatgatag tcaacaggga gcgttaacca
    11221 ttgcttttag tgatgatggt gttggcattc cggatgacga aattgaaaag gtttttgaac
    11281 caacgttttc tactaaagaa aatggtatgg gattggggct tgctattgtg gaaaagatta
    11341 ttattgatca taagggcact atcacctgca gttccacatt aaaacagggg actaaattta
    11401 ccataactat cccccagcaa taaggttata actatggcaa agattttagt tgttgatgat
    11461 gaaaaaaata tattaaacac catgaaggca atcctgcagg atgaaggcca tgtggtgtat
    11521 actgtccaga atggaaaaga tgctctccag tttgtaaaat ccaatgaatg tgatgtggtt
    11581 tttttagatg tatggcttcc tgatattgac gggatggagg tcttacagca gataaaacag
    11641 ttgcagaatg atatcgcagt catcatgata tcaggtcatg gttcaattga tattgcagta
    11701 aaatctacac ggcttggtgc ttttgatttt ctggaaaagc caccttccat ggaacgtgtt
    11761 ataacctcgc tgaacaacgc cattgagcag atacagctta agcgtgaaaa tgttatatta
    11821 aaaaagaaca tcaccatgga agatgagatg attggcaatt ccatcccaat gcaggaggtt
    11881 aaacgcatca ttgatactgc tgctgcaacc aatgctcgtg tttttataac gggtgaaaat
    11941 ggcacaggca aggaactggt agcacgagct atctaccgaa aatcaaaacg aagtgataaa
    12001 ccattcataa aggttaactg tgcagcgatc cctgatgaac tcattgaaag cgagttgttt
    12061 ggccatgaaa agggttcatt taccggtgca cttgcccggc ggttgggaaa atttgaactg
    12121 gctgataagg gtacaatctt tcttgatgaa atttgcgata tgagtcagag cgctcaggct
    12181 aaagtattgc gtgtattgca ggagcaacag tttgagcgag taggcggcaa tgacgttata
    12241 accgttgatg tgcgggttat tgctgcaacc aatgttgatg taaaagaggc tattgagcag
    12301 ggacgtttta gagaggattt atattaccgg ttaaatgtaa ttcccattta tgtccctcca
    12361 ctttctgagc gcaaggacga tataccgctt ttagtggatt attttcttga aaagtttgct
    12421 cgtgaacatg gtctgggcgt taaacagatg agcgacagcg ctatgcattt tatggtaaac
    12481 tatgcatggc cgggcaatgt gcgcgaactt aaaaatgtca ttgaacggct taccataatg
    12541 gtgccatcag aggttattga gcttgatgat ataaagaagc atattgaatc gtatgattac
    12601 gaagatacca tagtgaagga ttcatcaagt ttaaagaaag ctcgagagca atttgaaaaa
    12661 gagtatatta taaaggcatt aaaacagttt gataaaaacg tatcagcaac cgccaaatca
    12721 ttggggattg agcgcaccaa tcttcacagg aagatacggc agtatcatat caatgttgat
    12781 aagttataga gtatgtacag cacattaaaa gaagtattgc aaacctatcg taactatgct
    12841 caccgtttac ccatggtaat agcatacgaa gatgaagtaa ttcctttgac agttaacaaa
    12901 gcgtatcatg acactgcgca ggatgtgcag gatacaacag ctactaccgt acatgaagag
    12961 attgaacggt gtaatcaatg tcaatccacg cataagattg ccatgcagag cagattgact
    13021 gaagcggcta tcatgattgt gctaaatcca cctgcattaa tttcctcagt tgaaaaaaag
    13081 gtattgcagc ctcaggttga taccatgctt cagcgcatga tgaaagccat tgatgtccaa
    13141 atggaaaact gttacattac ccacatgatt aaatgtgaaa gccagaaaca attacccgga
    13201 acgatgtttt tgcactgtca gcaatttctt gttaaagaga taaatcatgt taatccacgc
    13261 gtcattatag ttatgggcga aatgctcccc atgcggaaaa taataaaaag catggatgcc
    13321 gttcaatggt ttggcataga gcacccaata acattaatta aaaatcctga attaaaaaaa
    13381 gatgcatgga aaaccctcaa attagtcagg gcaacattgg atgaaaaata gcgatatgtt
    13441 tgctgatgtt gccgttggat tgcctgttga aggtttgttt acctatagca cagcagagtt
    13501 cccgatcaca aaagggatgc gggttgtcgt tgacttcaac aatagaaaaa taatcggttg
    13561 tgttgtcaat gttcattcac aaaaacctga ttttgaaatt aagcctgttg caaaaataat
    13621 tgacgatgaa ccaatctttg atgagcgcct gcttgctatg gcgcaattta ttgcgcatca
    13681 ctatgtatgt tattttggcg aggctgtgag cactaccatg ccatccggaa aatcgtataa
    13741 aacaagacaa aagacttttt ttgcgggcga tagttactct gaaattaccc ttacccctga
    13801 acaggaagac atacagcagg caatactgca gcaagggcaa aaggcacact gcatctatgg
    13861 tataaccgga agcggcaaaa cagaggttta cattgcatta gccagggaga tgattaggcg
    13921 caatcaatcg gtgttatacc tggtgcccga gattggtata tcatcgcaga tgtttatgcg
    13981 tcttaaggaa gtatttggca acagccttgt gatgtatcac agcggcatga gtcctaatga
    14041 gcggttgtta agctggcggc gcttttacca tggggatgct gcgattgctg ttggtactcg
    14101 ctcggctata tttatgcagg caccacatgt tgggcttatc attatcgatg aggagcacga
    14161 tgcctcgtat aaagaaaaca gtacgccacg ctatcatgca cgcaccgttg catggtaccg
    14221 ccataaaaat gaaggcagca tgcttgtatt gggttcggca actccatcac tggaaacgct
    14281 gtacgcagtg cagggaaaaa gaataatgat gcatacattg cacaggcgtt ttggcaatgc
    14341 aacactgcca cagcttgaaa tactgcctgt aaaagcttca ggtggcaata aaataattac
    14401 caatcaactg ctgatggcaa ttaaaaagac aatcgaagca aaacgacagg ttatattact
    14461 ccttaacaga cgaggatttg cacctcgtgt tatatgtgat gattgcggca tgagcgtaca
    14521 gtgtcctcat tgcagcataa gcatgaccta tcataaaaaa aaattgttat gccattattg
    14581 ccattatgag tccaccatcc ctgagcagtg tgaacactgc ggttcaaaaa atttaatata
    14641 tgttggcagc ggcactcagc gcatagaaga actattaacc aggatgttcc ctctggctac
    14701 tattgtgcgc atggatcagg atactgcacg aaagaagaac tttgttgctg aggttgtcag
    14761 caacatgcag caggggaaga ttgatattct gcttggtact cagatggttg caaagggatt
    14821 tgattttccc aatgtggcac tggttggcgt tattatggca gatattgggt tatcgcttcc
    14881 ggatttccga gcagcagaac gcatctttgc attgctggta caggttgcag gaaggtcggg
    14941 caggggcgat attgcaggta aagtcatagt gcaaacaatt aatcctgaca atccgctttt
    15001 taatcatctt aaaaattatg actacattgg tttttgtcaa agggaactat cgtaccgaaa
    15061 agccttgaac tatcctccgt atgtacggct ggcacggctg gttatccgtg gtaaagatga
    15121 agatgaggtg gcacaggttg caacgcttta taaaaaaaag ctggatgagc tgttagcgca
    15181 ctatcctggc atcactattt taggaccgtc acaggcaccg ctgtataaaa tagcttcata
    15241 cttccgctat cacattgtat tgaaatcagg cagcgttgat gcattaagtg ggatgatagc
    15301 acaatgcaaa aaaaatatgg atgtgcataa cgcctatata gaagtagata ttgatccact
    15361 tgatttaatg taagcattta ttttttttct tcaaggacaa ttttattgcc ggcgatagtt
    15421 acataaaaaa tgtcatcact ttcttcaagc ctttccagta tggtaaaagc tgctgagcgt
    15481 ttaaaccttg caagcatggt gttgttgcga acatagcagt aaagctcagc agcttttctg
    15541 atatgaagcg tgtcaataga aagttcctct tcctcgccgc tgctcaatgt aaggataaca
    15601 tgttcaaagt cagcaaagcc ttcaaagcgt gcaccggtaa caaagatagg tgcgtcctct
    15661 acaacgatag gataggtaaa attggcgtaa tgcaatacgt atgttccatc gcgtgtaata
    15721 gtaattgatg aattaaaaaa acgtataata cgctgattgg taaaaggctc gccattgtga
    15781 aaccagttgc cttcagcatc aaggcgtatt tcatcaattg catgctcttc aaataatttt
    15841 tttatttcat cagggatttc aggaaactct ggcatacagt atattcacct ctcaaaaaag
    15901 atagtgtata gtatcactgg ctgtgtaagg ttacctattt tgtatcatga ttatatgcca
    15961 tggtaggtac atacatgctc gtgtgcaaac acatttttct tttatactgc catctttaag
    16021 ggagttgggt agtattgtat tgctgattca ccgaaataga agtatacacc tacacaaccc
    16081 ccttgtgttc ccccctttgt aaaaggtggg aattgtgtgg aggttaaatc ctcattattt
    16141 cttcgacagc tctttttcat acgcttccgt tgctacttta cggtttaagc gtatctgctc
    16201 gtttgaaggg tctaactccg aagcttttgt taaaaattgt aatgattttt tatagtcatg
    16261 ctttgcatag taacacctgc ctatagtatg gtaggcttgc gcaacaattg cgtcgtcatt
    16321 ggatacctcc agcacctgat agaatatctc catagcctta tcgtatttat gctggttgaa
    16381 ggcaattgtt ccaagtgcta acaatgcttc cgcatcattg ggagcaatga gcaatgcctt
    16441 ttgcaacgat tgtatagcct gtgcctgctg tttttcatct tttgatgttg ataaaataat
    16501 ccctgtgtta acataggctt tttttgcata gtcattgttt tgatttattg caatgaccct
    16561 gttaaatgct tccagtgcat agtcatctct gcctttggag tggtacagca taccaagcat
    16621 aaactgggct tcctgtaaat gaggccacag gcgaacaacc tttatgtatt catccagcgc
    16681 taaatcaaga tacccctttt cataatatag atttgcaatt gcaaggcgtg gttttggatt
    16741 atcaggatta ttccgggaag cttcgttaaa tgctgcaact gccagttcag gcttaccggc
    16801 atgtttatag gcaatgccca tattatacag ggtggagcta tcgtttgggt ctattgccag
    16861 tgctttttta taataggcaa cagcttcatc aaagcgatgc tggtcgtcac gaatgtttcc
    16921 catattggcg taggcaacgc gtgcattttc tgaaacgggt tcatagatgg taatttgctt
    16981 atagtactca aacgcagtat ccagatcccc tttacgatag tacagctcgg ctaaacgtgc
    17041 aatgatcgca acattcctgt tttcaaccgg acgtaaacgc tctaaaactg caataccttc
    17101 atcatagcga tccagggatg catacgcctg cgataagcct tctaaaagtt cttcttcata
    17161 gatgccggat gattcggcag ctttaaattc tgccagtgct ttgtcaaatt gctggcgctg
    17221 catataaaca atacccagat tgtagtggtt gattggttca ttggggtcaa gctgtatggc
    17281 aaggtttaaa tatttttcgg ccatggcata gtccctgcgg ttggtgtaaa acgagcctaa
    17341 acggctatat gctgattttg caatagcacc ggtagggtca gcagcggcag cttttttaaa
    17401 atattcggtt gcagatagct catcgccggt ttttaaaaat gttaatccgg tgttatagag
    17461 ggcttgtgga ttatccggtg caatatccag agcctttgta taactttttg cagcatcagg
    17521 gaatctgccc atatcaaaat aaatatttcc ctgtaataat agtaaatccg gattattgcc
    17581 atcaatggcg atagctctgg taatagtggc taaagcgtcg ggatagtctt ttttatggcg
    17641 atacgctagt gctaaatttt taagtacggt ggtatcatca ggtttgtact ttaatgctcg
    17701 tttcaatgtt tcaatagctt tattgtagtc acccttatca tcgtatatca tgcccatgta
    17761 ggtaagtgct aaagccttat cattatcaga tgcatccgat tcaactacct gattaaactc
    17821 atcaatggcg ctggtataat tttttttaaa atagtatgtt ttcccgcgca gtatatgctg
    17881 attttgtgat atgtcctcag gggcaagctt ttcatcgctt aagccgggta aaagtttata
    17941 ggcattgttg cctgattttt gaatgctgga taaaaatgat tctggcttct ttacaagcga
    18001 tacaatacca acaacaagag caacgcctgc aatgagcacc attgcggcaa taaggggatg
    18061 aagttgtttt ggtatgctga gatgtaattt tttgctcttt ttttcttctt tattttcttc
    18121 ttcaatggaa aggatacgca tcccctgtgt atggctatct tcaggaataa tggtgaatct
    18181 attttttttt accggaggca tggtatttac ctctttttgc cttcatgaat ccgtatggca
    18241 tctaaaattc catttataaa ttgtcctgaa ttttcgcttc cataaatttt gcccaattca
    18301 atgccttcat caatggttac cgcatgcggt atcgcatcac aatagagcag agcatataca
    18361 gaaattcgca atatggattt atcaaccgga tttatgcgtt caaatttcca gtttcgggaa
    18421 tactgcttaa ttaaattatc aatacaatca atattggcga tagttccggt aataagattt
    18481 aatgcaaaat ccatgatatc ttcagggatt tctttatcaa cccaatcaag ccgtgttaat
    18541 tcatcaacag gagtgcccac agtctcgtac atgtagagag cctgcattgc aaattcacgg
    18601 gcttttcttc gatgacccat tatcgtaact gtttatacaa ttgtgccatt tcaatagctg
    18661 ccattgccgc atcaaaaccc ttattgcctg attttgtacc agcacgctca actgcctgtt
    18721 caatggtatc ggtggttaaa acgccgtaga taaccggaat gtttgattgc aatgaaacct
    18781 gcgccacacc ttttgatacc tcggcagcaa catagtcaaa gtgaggtgtt gcaccgcgga
    18841 taacagcacc caggcatacc actgcatcat aattgccctt tgacagcttc attgcaatgg
    18901 gtggtatttc aaatgcgccg ggcgtccaga ccacgcagat atttttttca tcagcattat
    18961 gacgctgtaa gcaatctaaa gccccggata aaagtttgct ggttataaat tcattaaaac
    19021 gcgaaacaat aatagctatt gttaaaccgg atgcgtctaa ttttccttct attgtgttca
    19081 tacaaaacct gctcttcaaa gattttatta tgtgcgatag ttcctgatat cagcattgcc
    19141 atcgttatcg ttaatagtag aaagtgtgtg atatcatgct catttaagtc aagttatttt
    19201 ttaacctggt tactgtcatc ttttcatcag cactgtataa cgatacttta tgtttacact
    19261 gtgcaatagt atgacagcaa gcaatccatg taacagtaac tatcgcccac cacacctgcg
    19321 atagcttatt tactcattgt tgcctgaaca aactccaatg ttttacgaac atcatctttg
    19381 aaaaagcact gcaacccctg ggtccagctt ccgtcggtaa ggtctttgcg aatacggtcg
    19441 gcagtttcct gacgcacgca taactttaac ggtgtaaacg cattgccagg attatttttc
    19501 cataggttta taagctcttt tgcccggctg ataagcttat cctcgtcagc cagctcgtca
    19561 accagcccca tttttaatgc ttcgtcaggg ctaaacatgt tgccaaagta catgatgtcc
    19621 ctgaattttt tgtcactgtc aagcccaaaa cgcaccacat tccactgggc aactgatagc
    19681 ggaagcccta tctttatctc gctcatcccc attttaatct ttggatgatt cttcatgata
    19741 cggtaatcac aagccatggc aaaaatcata ccgccggcaa cggtatgacc gttgagtgcg
    19801 gcaataacag gcttttcaaa cataaaaagc tctaataaaa tttcttcttc aacatcaaaa
    19861 aacttgatta cctcgtttat agtggtaaag cttaaaaaca tgggaaggtg aaaaccagat
    19921 gaaaaggttt ttccctcgcc tgtgaggata acacctttaa tggttgcgtc agatgctgct
    19981 tcttttaaat actttctcag gtcattcagt actgtatcgg taagcgcatt tgtcttgcca
    20041 tcattaagcg tcaggatgta cacattatct tcaacctttt ttgtaatcat acaaagcccc
    20101 ctataggtaa tgattaatca taattacata tcagtaacac aatagaaaca gttgccgtac
    20161 atgtaaagaa taaattttaa aacatgccat aattaactac attgttgttt tattatttta
    20221 ccatattgta ccattgggtt atctgataat agcatgatgc accacaccgt cattcaagga
    20281 ttaatccgga attcaaatgt gcaatgctat atcctgaatc agcgctcagg acatgattca
    20341 ggatgacagg cggtactacc ccccttgtgc cccctttata aaagctggaa attgtgtggg
    20401 tgagttcatt ggcacaaaat aggttttatt cttcacaatt gtttttacaa tataatttcg
    20461 tagtaaccac attatgctta cttgataagt tctgcattct tcatggtaaa atttcttcaa
    20521 attgtgtaag gatggaattg atacttaata attggggtca gagccctgtt aacttcaccc
    20581 tttcgttcaa aggggagtag ggggagtatg taaactctgc atgccagaag tttattccta
    20641 tagaccattg agttatagac gcaccgacat cataatttac gatacaagaa gttgatgcag
    20701 gaggggcatt gcattcatta ttgattttcg tatatacatg gtaatacata gaataaccca
    20761 cgcatcaaat cattatgcgt tctgttccgg gcataaaaaa agtcttttta gcttatggcc
    20821 atagacgtat aacagcaaag tcataattac ttccattgga gctataacct gcgacaagga
    20881 tttta
//
LOCUS       DAOSOX010000023        19468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2806_length_19468_cov_8.849637, whole genome shotgun sequence.
ACCESSION   DAOSOX010000023 DAOSOX010000000
VERSION     DAOSOX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19468
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_19468_cov_8.849637"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1773
                     /locus_tag="PLX22_03145"
     CDS             202..1773
                     /locus_tag="PLX22_03145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT18933.1"
                     /translation="MAKNNEHILAIDAGTQSIRAIVFNLQGNIVDMVKTPIEPYFSNY
                     PGWAEQEPEYYWKTLCQTCKKLLARGKVKKDALRGVTLTTQRDTMINLDKNGKPLRPA
                     ILWLDQRRADKSIVKLPFYMKAGLRLVNMLDAVEYFITESEANWIRQYQPEIWEKTYK
                     FVFLSGFFTYRLTDDYVDSTGCQVGFVPFDYKKQSWAPPADFKWRLFPMNKDNLVRLV
                     KPSEVLGYITKKAANATGIPQGLPLIASAADKACEVLGSGCLTPNIACLSYGTTATIE
                     TTNTRYVEIIPFFPPYPSAVPGAYNTEVIIYRGYWMLSWFVKEFGAREEMQAKKMGIK
                     PEALFDRLVKEIPPGSMGLTLQPYWSPGIKIPGQEAKGAIIGFGDVHTRVHIYRAILE
                     GLAYALREGAERTQKRNKVPIEKVRVSGGGAQSDVAMQLTADIFNLPAERPHTFETSA
                     LGAAIDAAVGLKLYPDFKTAVAAMTRVGKVFYPNPKDVAMYDALYKNVYQKLYIRLRP
                     LYEEIREITGYPEKR"
     gene            1882..2085
                     /locus_tag="PLX22_03150"
     CDS             1882..2085
                     /locus_tag="PLX22_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18934.1"
                     /translation="MEIVSGILCEVCHENEAVVRCDGCGKALCKKCRLFDIWCYGCGH
                     GDTKAFCKQCNENPAINIWKTPV"
     gene            complement(2090..3010)
                     /locus_tag="PLX22_03155"
     CDS             complement(2090..3010)
                     /locus_tag="PLX22_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT18935.1"
                     /translation="MAIPTSRGNAITTAGIDEKSCTGCELCVTACPEKNIFIKDKKAA
                     FQLNPYFGCIGCGHCMAICPKGAIAIHGRDIDASNVFPLSYTTNAATYAQLLSLMQKR
                     RSTRLFKDTPVSKDSINKIIKAAQTAPMGIPPSEVNIIVWESKESVLTFTKDFCSLLE
                     QMNWMVSDWFLFIMKIFLSKTNYLFFKDFVKPLVNIYVDNMKHGINYVTYDAPATIYF
                     YGSPYCDYADPIIAATYAMLAAESLGLGTCMIGGIHPFLQNGSKAKAFREKYGITYKS
                     KTGLFVIFGYPKIKFKKGIKRSFASVTTIP"
     gene            complement(3023..4231)
                     /gene="icd"
                     /locus_tag="PLX22_03160"
     CDS             complement(3023..4231)
                     /gene="icd"
                     /locus_tag="PLX22_03160"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOT18936.1"
                     /translation="METIKYENGIIEVPDNPVVLFIEGDGTGPDIWKATQIVLDAAVN
                     KAYNGKRRIHWQEILAGEKANTSTGSWLPDETLEKIRQYKVAIKGPLTTPVGGGIRSI
                     NVTLRQVLKLYACVRPVRYLTGVPSPLKKPERVNMIIFRENMEDLYAGIEWKQGTPEA
                     KKVIAFLNNEMGQHLSDDTGIGVKPISMSNTKNIVKSALRYAIENKRKSVTIMHKGNI
                     MKFTEGAFRDWAYQTAQEEFADYIITEDELWSTYQGKQPAGKIVIKDRIADALFQMVL
                     LRPDEFDVIVTPNLNGDYISDALAAQVGGLGMAPGANIGDDAAIFEATHGTAPKYANQ
                     DKVNPGSLILSGEMMLRFMGWTEAADLIIKGLETTIQQKRVTYDLERQMEGATLLKCS
                     EFGKAVADNM"
     gene            4620..5537
                     /gene="trxB"
                     /locus_tag="PLX22_03165"
     CDS             4620..5537
                     /gene="trxB"
                     /locus_tag="PLX22_03165"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT18937.1"
                     /translation="MKELIIIGAGPAGLAASIYAVRSGLDTLVLEKISPGGQVMLTHA
                     IENYPGFIEPVEGWQLAYNMEQQARRLGVAIESKDVVSVEKYDDMFHVKTTDGAVIEG
                     RAIIAASGASLKRLGVPGESEFTGRGVSYCATCDAAFFKDRVTAVVGGGNTALEEALY
                     LTRFASKVYIIHRRDEFRGQKILQDRVLSNPKIEPIFDTLVTQVHGDEKVYSITLQNK
                     KTGESRELAIQGLFIFIGNTSNTTYLPEQVLNDSGEVKVDMKMQTPISGLFAAGDLRE
                     NSLRQVLMAAADGATAAISAYEYITHKDW"
     gene            complement(5578..6357)
                     /locus_tag="PLX22_03170"
     CDS             complement(5578..6357)
                     /locus_tag="PLX22_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOT18938.1"
                     /translation="MEKPYCFIIPHGGYQIPEELAQLCVLNEFELFFEADTCANDLFD
                     LQQIGTVIHTSIHRLFIDVDRDPYTLPPRSSNGVMKMHTSQKKLMFTSGVFPDEIAIA
                     AMMKRYWFPFHNSIEHTVKSTRCIIVCHTTMPVAPIVARDAGIPRPIIAIEHGTTNGS
                     DIITCEENTALKLLSFFQKAFGSEDCTVAAPFVIAKPAQRYIMQRYAKHQIPIIRVAL
                     SKALFLNEKYFDPHSLSVDSNRIQHIQERLHDCFSKFARYI"
     gene            6500..7006
                     /locus_tag="PLX22_03175"
     CDS             6500..7006
                     /locus_tag="PLX22_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT18939.1"
                     /translation="MRPYKIDTLGEDILEILSHDAKATVEDIARQLDVSIDKVKKSIS
                     RLEKDRIILRYKTHINWQRVKHDEVRALIEVKVTPQRGVGFDSIAEVIYRFPEVTSVY
                     LLSGGYDLLVQVEGPSLREVAMFVAEKLATIDHVQSTVTHFMLKKYKEDGDILVEQPE
                     GKRLPVSF"
     gene            7013..7666
                     /locus_tag="PLX22_03180"
     CDS             7013..7666
                     /locus_tag="PLX22_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOT18940.1"
                     /translation="MFSLNTVYQKLFACYGPQHWWPGETPFEITIGAILAQNVSWLNA
                     AKAIASLKAHNLLTPHKLFKEDSVAIAHHIKPSGYYNQKAFAIQSFLQWFHRYDFSFD
                     ALNAIPTELLRKELLSIKRIGYETADSILLYALHRKVFVVDAYTKRIFTRAGFLTGNE
                     DYMAIQAMFHEHFTGNTYDYNEFHALIVKHGKDRCTKKPACNECCIADECKEYDVAK"
     gene            7931..8101
                     /locus_tag="PLX22_03185"
     CDS             7931..8101
                     /locus_tag="PLX22_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18941.1"
                     /translation="MNTNAPKSATWWIAVIIGVLGIVGKFVHISVLEAYSWWLVCIGF
                     IVLALATAIKGL"
     gene            complement(8146..8880)
                     /locus_tag="PLX22_03190"
     CDS             complement(8146..8880)
                     /locus_tag="PLX22_03190"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOT18942.1"
                     /translation="MIQKSLRWIALIASLCFFSNPGGYPIKTIPYYSLYTLARASAPA
                     PITVSHSKPGMPAIIRGTLITYKNRNARKVYIAGDFSYWKRQPMERSNNGVWFYIIPF
                     DDEERIIRYKLMVDGIWINDPVNMDIDDDNTGSYYSIVRQDKLPESKQISFHIVNEST
                     RLVEFRVYAPHATIVALAGDFNNWNTEADLLKKDKNNIWRLQKRLPEGVCRYNFVVDG
                     TWQLDLYNPHTASNAAGMLCSVISLP"
     gene            9077..12079
                     /locus_tag="PLX22_03195"
     CDS             9077..12079
                     /locus_tag="PLX22_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18943.1"
                     /translation="MAVSEEIKNLTPEEEAEVKQIVDAFDSELSSIAQMRGKIGILHA
                     DSTDADFTITPGMRSKEMETTSAPVEGFEETEDIVDITDMIEEVPEDVVLASEAVLPE
                     VEETIESVEELPSEAVEIAPEEVLPPEFKEEFPTEEVPERKASKKEAISPLDELEELT
                     QFEPETVEAHDIRTDEFVTEEPRIHGDVHLDEDIHLSELETEEISEISEIKAEELPQV
                     DISTMGMEEEMPEPTAFEEEIKEEPARARFETPSFDELDTFEEPLKKSEKIESVIDEF
                     KPEFEGLSVEEEEPAPIEEGVEISDAELRRLKKALLLLHPALRKKIKEIILRDELSPQ
                     DTRKLIDLIISGKPEENIQRFIEKRLNITLELEEEVPSHRRVITARPEYTREGMERQK
                     RLLRQTKTLALAVVVGFTLTVTVYQYIYKPFMARRLISKGVALILEPGDPATKKVKDY
                     KKAEQLFEDVDAHYKKDYLYGYNAYARAYFKKREFDYAYVKLKRAYSINNRDVETLNN
                     TGYFFAKIPEHYYNRIQNDAIKTFAPKDTYVISQVDLAIALYNKVLALDPNNITAMFG
                     IGNAYLYQGQYLKSRQYFENIIKVNPDSWIGYAGLLNLFIERDNLPEVLSIHTDLRAR
                     DILTDIPSPLLAKLAHYYLGKNRSDTFNVRVDLGVQSPRLKDEEDNLIPACRGVLDAL
                     KEKDPDYPPLYVASAKLAAAQKNLKLTQMLLQEAEEKAQEKGERYFAALHLLGEYHYT
                     IKQPVEAYKYFNAALTAYQYPPEFTYEDFYYESETIGKTYAYLGNIFYYFFDKVKFRF
                     GDELDEALLDEDSDKMANLDIARQKYELALQENYSSPELRYNLGRIFYINQNYQQALE
                     QWINLYEDIVTNPQVMLAIGNAFYHMNNLEASKGEYLKMIAVFERKAESIRRPMPERN
                     EHIIIFQSLASAYNNLGAVYQIQGNETKSSIAYWKAIEYATRIGRENEFARVNLARGF
                     KPRVTPIMPILDESIPYSIDIYREDMRK"
     gene            12098..12229
                     /locus_tag="PLX22_03200"
                     /pseudo
     CDS             12098..12229
                     /locus_tag="PLX22_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
     gene            12371..15115
                     /locus_tag="PLX22_03205"
     CDS             12371..15115
                     /locus_tag="PLX22_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT18944.1"
                     /translation="MQTVKAYHADAVMQYDDNAIIEHIAQHGFATDSRFLLKTESYVL
                     SCAYANNKMLSLSNSRTRILPHQIESTYTVVNALHKRFLIADEVGLGKTIEAGLIIKE
                     LFYRFNYERILIVCPASLLYQWQYELKSKFNEDFIIADSRVYRSEKGNPWKLNKLLCS
                     IDFIKNKHFAGKLSQTYWDTVVIDEAHRLRRDANSQTKSYQVAEILAKNSKALLLLSA
                     TPFRGKLEELYYLLSLLDKNLLGPIHSFQETYCVPEADLTLLRDTISKVIIRRTKYEV
                     GGFTKRLAKTIRFELYPEERELYNATTEYVADQFNKAMQTNNRTIGFVMTVFQKLLDS
                     SSYALLCALQKRYQYLTEIYNNHHHAIQSMQEIDENVIEAEDVERIVQVESAELENEM
                     NTLATLITMAQSIVRSKKGEKLLQLVRKLFAEKHNKILIFTQFRTTQDYLAQLLSHYK
                     VVLFHGSMSRDEKETAIEYFKSEAAILICTEAGGEGRNLQFCNVLINYDLPWSPLKVE
                     QRIGRIHRFGQSSNVIIYNFATRNTVAERVLEVLQHKLKLFEESLGTPDIILGDITDE
                     RFFNNIFMELIAGLKNKKRVAKDIELHIQAAKASYEKMGQLAVTKKIDLNFDEYYTIT
                     LQERDFSNKRIENFVNTLKNIEPTAVDFLSVQNKKTRFYGVHHNPFTLQPVNAQGTFD
                     SAVALENESLEFLAFGHPVVDWCVEHCKEYTFGGLSGIKQIQYSYDCSGIVFNYLLTI
                     HAKETMQRIVPVFIPYNTMDDAADDATIEDIASESMRQFGIVSSVVDDAVVKYYQHNI
                     YSCYNQSRKVLDQKIHHIIAGIKESMLLEVDPYTERVEDHYSKEIAHVKQRLEWQECQ
                     QRWYDRDMHSAITRSRNTIKNLEEEKLQQLQQYKNFITIQWDAKLINAGLLICKQQLP
                     "
     gene            complement(15107..15421)
                     /locus_tag="PLX22_03210"
     CDS             complement(15107..15421)
                     /locus_tag="PLX22_03210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT18945.1"
                     /translation="MAEKRKYNRVQKKCKSVVESMEGVTYSTINDISEGGIFISTPEP
                     IHPGSTVDVELCLSPEEKITLKGTVRWIRIKEEDNKKAGMGIQFIPTHKEIEKLKKVI
                     SR"
     gene            15700..16299
                     /locus_tag="PLX22_03215"
     CDS             15700..16299
                     /locus_tag="PLX22_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT18946.1"
                     /translation="MHIIYLSKKVLFPHCTITVELKRSRFLYTLKPGDKVIVYPVKKI
                     ADFILMQGVATLAEIVKSELSNNKYTITFKGIKRVTIDKRHGIFQCSFTEIHERAIVT
                     NEVTEQIRKKSQELIFLINVDESDKLIHLLNFISDAVQLSDFIANYFILDYDKRVMLL
                     NNCDVSSRIDVILALLNDLIKNVKQKREKESYEKENISG"
     gene            16274..16738
                     /locus_tag="PLX22_03220"
     CDS             16274..16738
                     /locus_tag="PLX22_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18947.1"
                     /translation="MKKKIYQDEVLYTLAWSPMYQFDKYSITKIIPELAGIVALMEKR
                     SEETYHYILFYSCWRDGMRLGLKNLFDPIFTRHPDLAKAMEGRQLWYKYTVVDSNPFD
                     LQDILWWLIQNYEPELNDIQNYEDSKRYKNIYVREMELDRETVVERLPKYGF"
     gene            complement(16735..17334)
                     /locus_tag="PLX22_03225"
     CDS             complement(16735..17334)
                     /locus_tag="PLX22_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18948.1"
                     /translation="MDEEKQIKDIESLLRERKPLAEIAQYILEGAFVELDMERKDSLD
                     RFLEFVYSKVQRGNPYIIHLAYPTKRMTDTELETKVKELINVHLNPDIVLPLLKIFTR
                     NVHNADSNLYIAYLIEADNIIKSIFDTYLLFKKDIFETDKEKRTQNVRRMQQFLAHIE
                     SESASPLDAAARLKYILEFIALKQNVKHIYTMDDIALSH"
     gene            complement(17338..18189)
                     /gene="bamD"
                     /locus_tag="PLX22_03230"
     CDS             complement(17338..18189)
                     /gene="bamD"
                     /locus_tag="PLX22_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOT18949.1"
                     /translation="MKRCMILFFIFMVHAQQIDAHPLLELADTFMNNKEYYHAITECM
                     RYQFLFPDGKEYPQSMLIMSRAYFLGGNYYRATDIAYDCYKKYTGTMYGDSALYNLAV
                     MRLMEGSPFFAYRTYQEYFSIYPDGKYRETARLDFIYAQILLNNIHDAKNNVDAFIKQ
                     TSDEELRNEAMACLQLLHDYEKRPQKNIWVSATGSLLVPGFGHFYAGKYTVGVLSFVS
                     TTLCAALAYRGYTHDNNFQLIFFGLAGFTFYQYSLVSAVLNVGEYNSNSINQLKKAIH
                     ITVQKRF"
     gene            complement(18186..19010)
                     /locus_tag="PLX22_03235"
     CDS             complement(18186..19010)
                     /locus_tag="PLX22_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18950.1"
                     /translation="MSALLLLTLFFVHPDDAFVHKQQQLISTLYYTANYYQCIAESRR
                     YSMLTDDNMDYTIALCYCKGNQLATAVAILQKKEKKEYCDYLLLSKVYTSLQHYQDAL
                     QALSLCDTSAQDKPLQMLYYQNILAVYINRYDWDNALNVYTEHSDRLQQMHQIYTILN
                     AAQKEKINPYVAVTLSAIIPGSGHCYARHHADGLISFITVAVLSAATYIAYLNGSFAL
                     TCTLGTLTAIGYAGNVYSAYNSAIHTQETINNNYRTELNRYLEYNPEQFLPQWMKK"
     gene            complement(19007..19249)
                     /gene="yidD"
                     /locus_tag="PLX22_03240"
     CDS             complement(19007..19249)
                     /gene="yidD"
                     /locus_tag="PLX22_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT18951.1"
                     /translation="MHNAAGAIGYFFIRMFQVVISPQDGPNCRYRPTCSEYGRQAVMR
                     YGLVMGGFLAGDRIIRCNPFAIPNHDPVPQTLAEHP"
BASE COUNT         5995 a         3483 c         4120 g         5870 t
ORIGIN      
        1 caatggtcat tggttcctcc ttccagagta gtgtatatag taccgataac tatcgtacac
       61 tcattatttt gtaaagacat ttttaaaagc aagatactta caataaatat tgatagtttg
      121 ataaattttc tattgattta aatacctatc atgttataaa aaggtataaa aaataaatat
      181 cattagtggc aggtaatgga tatggctaaa aataatgaac acatactggc aattgatgca
      241 ggaacacagt cgatcagggc tattgttttt aatctgcagg gaaatattgt tgatatggta
      301 aaaacgccca ttgaaccata ttttagcaat tatccgggat gggcagagca ggaacctgaa
      361 tattactgga aaacattatg ccaaacctgt aaaaagctac ttgccagagg aaaggttaaa
      421 aaagacgcgc tacggggggt aacgcttacc acgcagcgtg ataccatgat aaatctggat
      481 aaaaatggga aacccttgcg tccagcaatt ctttggcttg atcagcgacg tgcagataaa
      541 tctattgtga aattgccatt ctatatgaaa gcaggtttgc gtttagtcaa tatgcttgat
      601 gcagttgaat attttattac tgaatctgaa gcaaactgga ttaggcagta tcaaccagaa
      661 atatgggaaa aaacatataa atttgttttt ctttcaggat tttttactta ccggttaacc
      721 gatgactatg ttgattcaac aggttgccag gttggatttg taccatttga ctataaaaag
      781 cagagctggg cgccaccggc tgattttaag tggcgactgt tccctatgaa taaggataat
      841 ctggtgcggt tggtaaaacc atctgaagta ttgggttata tcaccaaaaa agctgctaat
      901 gcaacaggca tcccacaggg gttgccgctc attgcaagcg ctgctgacaa agcctgtgaa
      961 gtattgggct cgggatgctt aacaccaaat atagcctgtt taagttatgg cactactgct
     1021 actatcgaaa ctaccaatac ccgatatgtc gaaatcatac cattttttcc accatatcca
     1081 agtgctgttc ccggtgcata taatactgag gttatcatct acaggggtta ctggatgttg
     1141 agctggtttg ttaaagagtt tggggctcgt gaggagatgc aggcaaagaa gatgggcata
     1201 aaacctgagg ctctgtttga tagactggta aaagaaattc ctcctggttc aatggggctt
     1261 acattacaac catactggtc accgggtatt aagataccag ggcaagaagc aaaaggtgcc
     1321 attataggat ttggcgatgt acatacacga gtacatattt accgggctat ccttgaagga
     1381 ttagcctacg cactgcggga gggtgcagag cgcacacaga agcgtaacaa agtacccatt
     1441 gaaaaggtgc gtgtgtcggg tggtggtgca caaagtgatg tagctatgca actgactgct
     1501 gatatattta atcttccagc agaacggcct catacctttg agacctcggc attaggtgct
     1561 gctattgatg cagcagttgg gcttaaacta tatcccgatt ttaaaactgc tgtagctgca
     1621 atgacacgtg ttggcaaggt gttctacccc aatccaaaag acgtggcaat gtatgatgcg
     1681 ctttataaaa atgtatatca aaaattgtat atacgattgc gcccattata tgaggaaatt
     1741 agagaaataa ccggttatcc tgaaaaaagg taatattttt aaagatgggt ttgcagttac
     1801 ccttccttta caggataaac ccgggataga agtgaatata gcaatttgca tcctgtggat
     1861 ttttttgata aaggtgacat tatggagata gtatccggaa tactgtgtga agtatgtcat
     1921 gaaaatgagg cggtcgttcg ctgtgatgga tgcgggaaag ccttgtgtaa aaaatgccgc
     1981 ttatttgaca tatggtgtta tggatgcgga cacggtgata caaaggcgtt ttgtaaacag
     2041 tgcaatgaaa atcctgccat taatatctgg aaaacacccg tgtaaaagtt tacggtattg
     2101 ttgtaactga agcaaagctt cgctttattc cttttttaaa ttttattttt gggtaaccaa
     2161 aaattacaaa aagaccggtt ttgcttttat atgtaatacc atacttttct ctaaaagctt
     2221 ttgctttgct tccattttgt agaaatggat gaatcccgcc aatcatacag gttccaagcc
     2281 ccaacgattc tgcagcaagc attgcgtatg ttgcagcaat aataggatcg gcatagtcac
     2341 aatagggaga gccataaaaa tatatcgttg ctggtgcatc ataggtaaca tagtttatgc
     2401 catgcttcat attatcaaca tagatattta caaggggttt tacaaagtct ttaaaaaaca
     2461 ggtaattagt tttacttaaa aaaattttca ttataaataa aaaccagtcg gaaaccatcc
     2521 aattcatttg ttcaagcagg ctgcagaaat ctttggtaaa tgttaaaaca gattcctttg
     2581 attcccatac gatgatattc acttcggatg gaggtatgcc catgggtgcg gtctgtgctg
     2641 ctttaattat tttgtttata ctgtctttgc ttaccggggt gtctttgaat aagcgtgtgc
     2701 ttcttcgctt ctgcattagc gatagtaact gtgcataggt tgcagcattg gtagtgtatg
     2761 aaagcgggaa gacattgctg gcatctatgt ccctgccgtg aatagcaata gcacctttgg
     2821 ggcatattgc catacagtga ccgcatccaa tacatccaaa gtaaggattt aattggaatg
     2881 cagctttttt atccttaata aaaatatttt tttccggaca tgccgttaca cataactcac
     2941 agccagtgca acttttttca tcaatgcctg ccgttgtgat tgcattacca cgagatgtgg
     3001 gtatagccat attattgtcc tgctacatgt tgtcagcaac agccttccca aattcagaac
     3061 atttaagaag cgttgctcct tccatctgcc gttccaggtc gtatgttacg cgcttttgct
     3121 gtatggttgt ttcaagccct ttgatgatca aatcagcagc ttccgtccag cccatgaagc
     3181 gcagcatcat ttcacctgaa aggataagtg aaccgggatt taccttatcc tgattggcat
     3241 attttggcgc cgtaccatgt gtggcttcaa aaattgctgc atcatcacca atgttagcac
     3301 ccggtgccat accaagccca ccaacctgag cagccagcgc atccgagata taatcaccat
     3361 tgagattggg tgtcacaata acatcaaact cgtcaggacg cagcagcacc atctggaaca
     3421 gtgcgtctgc aatgcggtct ttgatgacaa tttttcccgc cggctgtttg ccctgatacg
     3481 ttgaccagag ttcatcttca gtaatgatgt aatcagcaaa ttcctcctgt gcggtttgat
     3541 atgcccaatc acgaaatgcc ccttcggtaa atttcatgat attgcccttg tgcataatgg
     3601 ttacgctttt gcgcttgttt tcaatagcat agcgtagtgc acttttaaca atatttttag
     3661 tattggacat gcttattggt tttacgccta ttcccgtatc atcactcagg tgctgtccca
     3721 tctcgttgtt taaaaaggct atcacctttt ttgcctctgg cgttccctgc ttccattcta
     3781 taccagcata caggtcttcc atattttccc tgaagataat catattgaca cgttcaggtt
     3841 ttttcagcgg gctgggtacg ccggtaagat acctcacggg acgtacacag gcatagagct
     3901 tcaatacctg acgcagggta acattgatac ttctgatgcc accgccaacc ggtgtggtga
     3961 gcggaccttt tattgccacc ttatactgtc taattttttc aagtgtttca tcgggcagcc
     4021 agctgccggt gctggtgtta gctttttcac cggccagtat ttcctgccag tgtattctcc
     4081 ttttcccgtt atatgccttg ttcacggctg catccagcac aatctgtgtg gctttccaaa
     4141 tatcgggacc ggtgccatcg ccttcaatga ataacacaac aggattatcg ggtacttcaa
     4201 taatgccgtt ttcatatttg attgtttcca tagcctctct cgtatgcaat atgatttaat
     4261 tatcagtaac tatcgcacat aaaaattttt ttggcgatag ctcctggtat caactcgtta
     4321 ttaaagatta ctgctttatg cagaaatagt ggttgtgtta aataaagaaa gtatagcacg
     4381 aataaagtat ttgacattag tttttggaac aaaacagaat gtcacaataa aaaaatggat
     4441 gcgatcattt ttttagacac agtattttag aaaaagctta aatattgtga ttcaatgata
     4501 ggagatacag cttatgtttt tgtcatcaac atgcacggcg atcaatagtg tttcccctgc
     4561 tcgtacaaag ggaaggggca ggtggggata atgctatgta tggatgagag gtatatttca
     4621 tgaaagaact cattattatt ggtgccggac ctgcaggatt ggctgcatcc atatatgcgg
     4681 tgaggtcggg acttgatacg ctggtgcttg aaaaaatttc acctggcggg caggttatgc
     4741 ttacccatgc gattgaaaat tatccgggat ttattgagcc agttgaagga tggcagcttg
     4801 cctacaacat ggagcagcag gcacgtcggc ttggcgtagc catagaaagc aaagatgttg
     4861 tgtcagtaga aaaatatgat gacatgtttc atgtaaaaac aacagatggt gctgttattg
     4921 aggggcgggc tattattgca gcttcaggtg cttcgctcaa gcgtttagga gttcccggtg
     4981 aaagtgaatt taccggcagg ggcgtatcgt actgtgctac ctgtgatgcg gcatttttta
     5041 aagatagagt aacagcagta gttggtggtg ggaatacagc gcttgaggaa gcactgtatt
     5101 taacacggtt tgcaagcaag gtatatatca tccatcgtcg tgatgaattc cggggtcaga
     5161 agattttaca ggacagggtg ctatccaatc ccaaaattga gccaattttt gatacactgg
     5221 taacgcaggt acatggtgat gaaaaagtgt acagcataac gctgcagaat aaaaagacag
     5281 gtgaaagccg tgaacttgca atacagggac tgtttatctt tatagggaat acttccaata
     5341 caacatatct tcctgaacag gtgctgaatg attcaggcga agtaaaggtt gatatgaaga
     5401 tgcaaacacc catcagcgga ctttttgcag caggagattt gcgtgaaaat tctttacggc
     5461 aggtactcat ggctgctgct gatggcgcaa cggctgctat tagtgcttat gaatatataa
     5521 cgcataagga ttggtgacgg tttgtcgttg ttatatgcag tattacattg tacacaatta
     5581 tatataacga gcaaacttac taaaacaatc gtgcagacgc tcctgaatat gttgtatgcg
     5641 gttgctgtca acggataagc tgtgcggatc aaaatatttt tcatttaaaa aaagtgcctt
     5701 tgataaggct acccgtataa tggggatttg atgtttggca tatcgctgca tgatataccg
     5761 ttgtgctggc tttgcaataa caaaaggtgc tgctacggta cagtcttcac tgccaaaagc
     5821 cttctggaaa aaagacaaca gtttcaaagc tgtattttct tcgcaggtta taatatcgga
     5881 accattggta gtgccatgtt ctatggcaat gattggacgt ggtattcccg catcgcgagc
     5941 aacaattgga gcaacaggca tggtggtgtg acatacaatg atacagcgag tagatttaac
     6001 tgtatgctct atgctattgt gaaatgggaa ccagtaccgt ttcatcatgg cagctattgc
     6061 aatctcatca ggaaaaactc cgctggtaaa cattaatttt ttttgtgatg tatgcatttt
     6121 cataacccca ttgcttgagc gtggaggcaa tgtgtaaggg tccctgtcta catcaataaa
     6181 tagcctgtga atggaggtat gtatcaccgt gccaatttgt tgtagatcaa aaaggtcgtt
     6241 ggcacaggta tctgcctcaa agaacaattc aaattcgttg agcacacaaa gctgtgccaa
     6301 ttcttcagga atctgataac caccatgagg tattataaag cagtatggtt tttccattag
     6361 ttttatggtg ttatattggt atagtagtaa aattatattg actaattaag tataacacca
     6421 ttgaattgtg aagaactttt ttattatcgc aataatttat tgagagaata tacaacactt
     6481 ataaaaaaag gagaatgtca tgcgtcccta taaaatagat acattaggcg aggatatttt
     6541 agaaatactt tcacatgatg ccaaagccac cgttgaagat atagcacgcc agcttgatgt
     6601 aagtattgat aaagtaaaaa aaagtatcag caggctggaa aaggatcgca tcatattgcg
     6661 gtataaaact catataaact ggcagcgggt aaagcatgat gaggtgcgtg ccctcattga
     6721 agttaaagtg acgcctcaga gaggtgttgg ttttgactcc attgcagagg ttatctatcg
     6781 ttttcccgaa gtaacctcag tatatctgtt atcaggcggt tatgatcttc tggtgcaggt
     6841 ggaaggacca tcgcttcgtg aagtagcaat gtttgtagca gagaagcttg caacaataga
     6901 ccatgtgcaa tctactgtta ctcattttat gttgaaaaaa tataaagaag atggtgatat
     6961 actggtagag caacccgagg gcaaacgttt accggtaagt ttttagtttg caatgttttc
     7021 ccttaatacg gtgtatcaaa aactttttgc ctgttatgga ccgcagcatt ggtggcccgg
     7081 tgaaactcca tttgaaataa ctatcggcgc catacttgca caaaatgttt cgtggttaaa
     7141 tgctgcaaag gcaatagcca gcttaaaagc acacaacctg ctaacaccgc acaaactttt
     7201 taaagaagat agtgtggcga tagctcatca tattaagcct tcaggctatt acaatcaaaa
     7261 ggcttttgcc atacaatcat ttttacagtg gtttcaccgt tatgatttta gctttgatgc
     7321 attaaatgct ataccaacag aactactcag aaaagaactg ctgtcaataa agcgtatagg
     7381 ttatgaaaca gccgattcaa tacttttata tgcattgcac cgaaaagtat ttgttgttga
     7441 tgcatataca aaacggatat ttacccgcgc tggtttttta accggcaacg aagattatat
     7501 ggcaatacag gctatgtttc atgagcattt tactggcaac acttacgatt ataatgagtt
     7561 tcatgccttg atagtaaaac acggaaagga tagatgtaca aagaagccag cgtgcaatga
     7621 gtgttgtatt gcagatgaat gcaaagaata tgatgtggct aaataaataa taagtgatta
     7681 ttgtgacaat tcaatatgat gcataaataa aatttataat gactttcgta caattgccaa
     7741 aataaaatta taccactatc atcaccacta aacagcctct tgatgtttcg gatatctgtc
     7801 agtgtagcga taaaaatatt tttttgttgt gattgtattg tcatgtaaat ttttttttag
     7861 aaaataatat tgacaacacc atcattctga tggtatcttt taagcaataa aatataatag
     7921 gaggtttgct atgaatacca atgcacccaa atcagctaca tggtggattg ctgttattat
     7981 cggcgtatta ggtattgttg ggaagtttgt tcatatatcg gtgctggaag cctattcatg
     8041 gtggcttgta tgtattgggt ttattgtttt agcactggca actgctataa agggattata
     8101 gcatcaacag taactatcgc ccatgcagta gtgtattatt agcttttatg gtaaactaat
     8161 taccgagcac agcatacctg cagcatttga tgctgtatgg gggttgtaca ggtcaagctg
     8221 ccatgtgcca tcgacaacaa agttataacg gcatacacct tcaggcaagc gtttttgtaa
     8281 gcgccagatg ttgtttttgt ctttttttag caggtcagct tccgtgttcc agttattgaa
     8341 atcgccagca agcgcaacta tcgttgcatg gggggcatat accctgaact caacaaggcg
     8401 tgtactttca ttcactatgt ggaagctgat ttgtttgctt tcaggcagtt tatcctgacg
     8461 cacaatggaa tagtatgagc ctgtattgtc atcatcaata tccatgttta ccgggtcatt
     8521 gatccagatg ccatcaacca taagcttgta acgaattatg cgctcttcat catcaaatgg
     8581 tattatataa aaccacacac cattattgct gcgctccatt ggttgccttt tccagtatga
     8641 aaaatcacct gcaatgtata ccttgcgggc atttctgttt ttataggtaa taagagtgcc
     8701 tcttattatg gcaggcatgc ctggcttgct atgactgaca gttattgggg caggtgcaga
     8761 tgctcttgcc aatgtataaa gcgagtagta aggaattgtt tttataggat agccgccagg
     8821 attggaaaaa aaacaaaggg atgctatgag tgcaatccac cgcaatgatt tttgtatcac
     8881 accagacctc aggctcattg aaaattttac gctcatgaat tacttattaa tatgtcgata
     8941 ggtatagaga aaatatttat atgattttga tgaaaataaa tattaatata taattagaaa
     9001 aatgtatttg attttatgtg cagagtatga attatatgtt tatgtcacat tatatactgt
     9061 ataatgagag cccgttatgg cggtaagtga agagataaag aatctaaccc ctgaagaaga
     9121 agccgaagta aaacagattg ttgatgcatt tgacagcgag ttgtcttcca ttgcccagat
     9181 gcgagggaag attggcattt tgcatgctga ttcaaccgat gctgatttta ccattacccc
     9241 cggtatgcgc tcaaaagaaa tggaaacaac atctgcaccg gtagaaggct ttgaagagac
     9301 ggaggatatt gtagatataa ccgatatgat tgaagaggtt ccggaagatg tagtgctggc
     9361 atctgaagct gtattgccag aggttgagga aaccatagaa tccgtggaag agttgccttc
     9421 agaggctgtg gagatagctc ccgaagaagt attgccgccg gagtttaaag aagaattccc
     9481 gactgaagaa gtccctgaaa ggaaggcctc aaaaaaagaa gcaatcagcc ctttagatga
     9541 acttgaagaa cttactcaat ttgaaccgga aacagttgaa gcgcatgata tacgtaccga
     9601 tgaatttgtg accgaagagc cccgcattca tggagatgtt catcttgatg aagatataca
     9661 cctatcagag ctggaaactg aagaaatatc cgaaatatct gaaataaagg ctgaagagct
     9721 accgcaggtt gatatatcaa caatgggaat ggaagaagaa atgccagaac caacagcatt
     9781 tgaagaagaa ataaaagagg aacctgcacg tgctcgtttt gaaactcctt catttgatga
     9841 gcttgatact tttgaagagc ctttaaagaa atccgaaaaa attgagagcg ttattgatga
     9901 atttaaacca gaatttgaag gcttgagtgt tgaggaagaa gagccagccc cgattgaaga
     9961 aggtgtagaa atttcagatg ctgagttacg acgattaaaa aaagctttgc ttttgctcca
    10021 tcctgcttta agaaagaaga tcaaagagat aattttaagg gatgaactgt cgccacagga
    10081 tacacgtaaa ctcattgatc ttattatatc agggaaacct gaagagaata tacagcgttt
    10141 tattgaaaag cggctcaaca ttactcttga acttgaggaa gaagttccct cccaccggag
    10201 ggttattact gctcgccccg agtatacccg tgagggtatg gagcgtcaga aacggctgct
    10261 gcgtcaaacg aaaacgttag cactggctgt tgtggtaggt ttcacattaa cagtaactgt
    10321 ctaccagtat atttataaac catttatggc cagacggctt attagtaaag gggtggcact
    10381 tattcttgag ccaggtgatc cagcaacaaa aaaggtgaag gattataaaa aggcagaaca
    10441 actttttgaa gatgttgatg cgcactataa aaaagattat ctgtacggct ataatgctta
    10501 tgcacgtgcc tattttaaaa aacgtgagtt tgattatgct tatgtaaaat taaaacgagc
    10561 gtatagcata aacaatcgtg atgttgaaac actcaataat acaggatatt tttttgctaa
    10621 aattccggaa cattattata accgcattca gaatgatgcc attaagacat ttgcaccaaa
    10681 agatacttat gtaatatcac aggttgattt agctattgcg ctgtacaata aagtattagc
    10741 gcttgatcca aacaatatca ctgctatgtt tggtattggg aatgcttacc tgtatcaggg
    10801 acaatattta aaatcgcgcc agtattttga aaatattata aaggttaatc ctgattcatg
    10861 gattggctat gcaggacttc ttaatctttt cattgaaaga gataatttgc ccgaggtgtt
    10921 atcaattcat actgatctcc gtgcaaggga tattttaact gatattcctt cgccgctgtt
    10981 agcaaagctg gcgcattact acttaggtaa aaaccgctca gatactttca acgttcgggt
    11041 tgatttgggg gtgcagtcgc ctcgattgaa ggatgaagag gataatttaa tccctgcatg
    11101 ccgtggggtg cttgatgcat taaaagaaaa agatcctgat tatccaccat tatatgtggc
    11161 aagcgctaaa ttagcagcag cacaaaaaaa ccttaaactt acacagatgc tattacagga
    11221 agcagaagaa aaagcacagg aaaaaggaga gcgctatttt gcggcattgc atcttttagg
    11281 cgaataccac tataccatca aacagcctgt tgaagcatat aaatatttta atgctgcact
    11341 tactgcgtat caatatccac ctgagtttac ctatgaagac ttttattatg aatcggagac
    11401 aatagggaaa acatacgcat acttaggaaa tatattttac tatttctttg ataaggttaa
    11461 atttagattt ggtgatgaat tagatgaagc gttattggat gaagatagtg ataaaatggc
    11521 aaatcttgat attgcccgcc agaagtatga attagccttg caggaaaatt atagctcccc
    11581 tgagttgcga tataatttag gcagaatatt ctatataaat caaaactatc agcaggcgct
    11641 tgagcagtgg attaatctgt atgaagatat tgttaccaac ccccaggtga tgttggctat
    11701 tggcaatgcg ttttatcata tgaataattt agaagccagt aaaggtgaat acctcaagat
    11761 gattgcagtt tttgaaagaa aggcagaatc catacgaagg ccaatgcctg aaaggaatga
    11821 gcatattatt atattccaat cgctggcttc ggcatataat aatcttggtg cagtatatca
    11881 aatacagggg aatgaaacaa aaagcagcat tgcgtactgg aaagccattg agtatgctac
    11941 ccgtattggg agggaaaatg aatttgcacg tgtaaatttg gctcgtggat ttaaaccgcg
    12001 tgttactccc attatgccaa tactggatga aagcattccc tacagcattg atatctacag
    12061 agaagacatg cgtaaataac tttgtttcaa ttaagcacag gtgttgaaaa gaaaaaatcc
    12121 gtatttatta aaagcaaaat atatattaac ggctgaacaa attgttaaaa atataattga
    12181 tgcccatatt tcttcaaatg aagagacaat ctttggtgat tggctggaat gtgattatct
    12241 tagaaaaatt tacttgaata aagtaactca ttactacatt atgtctccta tattacaata
    12301 accagtgaaa taaacagtaa ctatcgcaca taactattgt attgaataat tatacatgac
    12361 aatgaatagc atgcaaacag ttaaagcata tcatgccgat gccgtaatgc agtatgatga
    12421 taatgccatt atagaacata ttgctcaaca tggttttgca accgacagcc gctttctttt
    12481 aaaaaccgaa agctatgtcc tttcatgtgc atatgcaaat aataaaatgc tgtcgctttc
    12541 aaactcgcgc acacggattt tacctcatca gattgaaagt acctacactg ttgtcaatgc
    12601 gttgcacaaa cgttttttaa ttgctgatga ggttggattg gggaaaacca ttgaagccgg
    12661 cttgattata aaagagcttt tttatcgctt taactatgaa cgaatcctta ttgtatgccc
    12721 tgcgtcgcta ttgtatcaat ggcagtatga gttgaaaagc aaattcaatg aagattttat
    12781 tatcgctgac agcagggtat atcgcagtga aaagggaaac ccatggaaat taaataaatt
    12841 gctgtgttcc atagatttta tcaagaataa gcattttgcc gggaaacttt cacaaacata
    12901 ctgggatacc gtggttatag atgaagcaca tcgcttacgg cgtgatgcaa atagccagac
    12961 aaagtcatat caggtagcgg agatattagc aaaaaatagc aaggcattgc tgttgctttc
    13021 ggcaacccct tttagaggta agctggagga gttatattat ttactatcgc ttttagataa
    13081 aaatttactg gggccaattc acagctttca ggaaacctat tgtgtgccgg aagcagatct
    13141 tacgttgctt agagatacca tttctaaagt tattataagg cgtactaaat acgaggttgg
    13201 cggatttaca aaacggctgg caaagaccat acgttttgaa ctatatcctg aagaacggga
    13261 gttgtataac gctactacgg aatatgtagc cgaccagttt aacaaagcaa tgcaaacaaa
    13321 taaccgcact attggttttg ttatgacggt attccagaag ctcctggatt cgtcatcgta
    13381 tgcattgctg tgtgcattgc aaaagcgcta tcagtattta acagaaatat ataataacca
    13441 tcaccatgca atacagagta tgcaagaaat agatgagaat gtcattgaag ctgaagatgt
    13501 ggagcgcatt gtacaggtag agagtgctga acttgaaaat gaaatgaata cactggcaac
    13561 actgataaca atggctcaga gtattgtccg cagcaaaaag ggtgaaaaat tattacagct
    13621 tgtacgcaag ctttttgctg aaaagcataa taagattctt atttttacgc aatttcgtac
    13681 cacgcaggat tatcttgcac aacttttgag tcattacaaa gtagtgcttt ttcatggttc
    13741 aatgagcagg gatgagaagg aaactgcaat agaatatttt aaaagtgagg cagcaattct
    13801 tatctgcact gaggcagggg gtgagggacg taatctgcag ttttgtaatg tgcttatcaa
    13861 ctatgatctg ccatggtcac cgctgaaggt tgaacagcgt attggcagaa ttcatcgctt
    13921 tggacagagc agcaatgtca tcatctataa ctttgcaacc cgaaataccg tggcagaacg
    13981 tgtccttgag gtgttacagc acaagcttaa actttttgag gaatcactgg gtactcctga
    14041 tattatttta ggtgatatta ccgatgaacg ttttttcaat aatatattta tggagctcat
    14101 tgcagggcta aaaaataaaa agcgtgttgc aaaggatatt gaactgcaca tacaggctgc
    14161 aaaagccagc tatgaaaaaa tgggacaatt agcggtaact aaaaagattg atctcaattt
    14221 tgatgaatat tataccataa cattacagga acgggatttt tccaataaaa ggattgaaaa
    14281 ttttgtaaac acgcttaaaa acattgagcc cactgcagtt gattttttgt ctgtgcaaaa
    14341 taaaaaaaca cgattctatg gtgtgcatca taatccattt acattacaac ctgttaatgc
    14401 acagggtacc tttgatagtg cagttgcgct ggaaaatgaa tcactggagt ttttagcttt
    14461 tggtcatcct gtggtggatt ggtgtgttga gcattgtaaa gagtatactt ttggtggatt
    14521 gtcgggcata aagcaaatac aatattcata tgattgtagt ggaattgtat tcaactatct
    14581 gcttactatt catgctaagg aaacgatgca gcgcattgta cctgtcttta tcccctataa
    14641 cacaatggat gatgcagcag atgatgcaac aatagaagac attgccagtg aatcaatgcg
    14701 tcagtttggt attgttagca gtgtggttga tgatgctgtg gtaaagtact atcaacacaa
    14761 tatctatagt tgctataatc aatcccgcaa agtgctcgat caaaaaatac accatatcat
    14821 agccgggata aaagaatcga tgctactgga ggttgatcct tatactgaac gggttgaaga
    14881 ccattactca aaagagatag ctcatgttaa acaacgtttg gaatggcagg aatgccagca
    14941 aagatggtat gatagggaca tgcacagtgc cataacacgt tcaaggaata caataaaaaa
    15001 ccttgaagag gaaaaactac agcaactgca gcagtataaa aattttatca ctatacagtg
    15061 ggatgctaaa cttatcaatg cagggctttt aatctgtaaa cagcaactac cgtgatatca
    15121 cttttttcag tttttcaatt tctttatggg ttgggataaa ttgaataccc atgccggctt
    15181 ttttattatc ttcttcctta attcgtatcc accgtacagt tcctttaagg gtaatctttt
    15241 cttcaggtga aaggcacagc tctacatcaa ctgtactgcc aggatggatt ggctcggggg
    15301 ttgatataaa gattccacct tcactgatat catttattgt actgtatgtt acaccttcca
    15361 tgctttcaac aacagattta cattttttct ggacacgatt atattttcgt ttttctgcca
    15421 tagtaacctc tgctaccatt aaatttatga tgaataattg atactgtaat gataaactat
    15481 ataaaataat attgcaatat ttttttatgc aactctctgt aaatggagag gtattgcttt
    15541 ttggggcgca ttgcaatacg cctctacttg ctgcgaggtt atcattgttt ataatgatat
    15601 aataggggta attttttgaa taacaaataa aatgattgca aatgttcata gcaattatta
    15661 aggttccctt ataaaaataa tactattcag gtgatatcgt tgcatattat ttatctttct
    15721 aaaaaggtgt tgtttccaca ttgtactatt acagtagaac taaagcgcag ccggtttttg
    15781 tatacgttaa aaccaggtga taaggttatt gtgtatcctg taaaaaaaat tgcggatttc
    15841 atattaatgc agggtgttgc aacattagcc gaaattgtta aatcagaact atcaaacaat
    15901 aaatatacta ttacgtttaa agggataaaa cgtgtaacaa tagataaaag gcatggaata
    15961 ttccaatgca gttttactga aattcatgag cgggcgatag ttactaatga ggtaacagag
    16021 cagataagaa aaaagtcaca ggaacttatt tttttaatca atgtagatga gtcagataaa
    16081 ctcattcacc ttttaaattt tatttctgat gcggtacagc tttcagactt tattgccaat
    16141 tattttattc tggattatga taaaagggtg atgttattaa ataattgtga tgtttcatcc
    16201 agaattgatg taatacttgc cttgttgaat gatttaatta aaaatgttaa gcaaaaaagg
    16261 gaaaaggaat cgtatgaaaa agaaaatata tcaggatgaa gtcctctata cgttagcgtg
    16321 gtcgccaatg tatcaatttg ataaatattc aataacaaaa attatccctg agcttgccgg
    16381 tatagttgca ttgatggaaa aacgtagtga ggaaacgtat cattatatac ttttttatag
    16441 ctgctggcgt gatggcatgc gtttgggttt aaaaaattta tttgatccta tttttacccg
    16501 acatcctgat cttgctaaag cgatggaagg aagacagcta tggtataaat atactgttgt
    16561 agattcaaat ccttttgatc ttcaggatat tttgtggtgg ttgattcaaa actatgaacc
    16621 ggagttaaac gatatacaaa attatgaaga ttcaaagcgg tataaaaaca tatatgtccg
    16681 ggagatggaa ctggacaggg agacagttgt tgagcgtttg ccaaaatatg gattctaatg
    16741 tgatagggca atatcatcca tagtatagat gtgtttaaca ttttgcttta gcgcaataaa
    16801 ttccagtata tactttaacc gtgcggcagc atccagtgga gatgcggatt ctgactcaat
    16861 atgagctaaa aactgctgca ttcgcctgac attttgagtg cgtttttctt tatcagtttc
    16921 aaatatatct tttttaaaaa gcaggtaggt atcaaagatt gattttataa tattatctgc
    16981 ttctataaga taagctatat aaaggttact atcggcattg tgaacattac gggtaaaaat
    17041 tttaagcaaa ggcagcacaa tatcaggatt aaggtgcacg ttgattagct cttttacttt
    17101 tgtttctaat tcagtgtcgg tcattcgttt tgttgggtat gccaggtgta taatgtaagg
    17161 attgcctcgc tgtactttgc tatagacaaa ttcaagaaaa cggtcaagtg agtccttccg
    17221 ttccatatcg agttcaacaa atgcaccttc aagtatatac tgtgcaatct ctgcaagtgg
    17281 tttgcgttct cgcaacagtg attcaatatc tttaatttgc ttttcttcat ccatacatta
    17341 aaaccttttt tgcactgtaa tgtgtatagc cttctttaac tggttgatgc tgttgctatt
    17401 gtattcacct acattgagca ccgcgcttac cagtgaatat tgataaaagg taaatccagc
    17461 aagcccaaaa aagataagct gaaaattatt atcatgggta taccctctgt atgcaagtgc
    17521 agcacacagc gttgttgata caaatgaaag cacacctact gtatattttc cagcataaaa
    17581 atgaccaaaa ccaggcacca gcaatgaacc ggttgctgaa acccagatat ttttttgggg
    17641 acgcttttca taatcatgca acagttgtaa acatgccata gcttcattgc ggagctcttc
    17701 atcagatgtt tgtttaataa atgcatccac attatttttt gcatcatgga tgttgtttaa
    17761 caaaatttgt gcatagataa aatccaaccg tgcagtttcc ctgtatttgc catccgggta
    17821 tatggaaaag tattcctgat aggtgcgata cgcaaaaaat ggcgagcctt ccataaggcg
    17881 catcactgca agattgtaca atgcactatc gccatacatg gtgcccgtgt attttttgta
    17941 gcaatcgtac gctatatcag ttgcccggta atagttgccc cctaaaaaat atgcccttga
    18001 cattatcagc atgctctgag gatactcctt gccatcaggg aaaagaaact gatagcgcat
    18061 acactcggtg atggcatgat aatattcttt gttgttcata aaagtatcag ccagctcaag
    18121 aagcgggtga gcatcaatct gttgtgcatg caccataaaa ataaaaaaca atatcatgca
    18181 acgtttcact ttttcatcca ttgcggtaaa aattgttcag ggttgtattc caggtacctg
    18241 ttaagttctg tgcgatagtt attgtttatg gtttcctgcg tatggattgc gctattatat
    18301 gctgagtaaa cattgcctgc ataccctatt gcagttaatg tccccaaagt gcaggtaaga
    18361 gcaaagctgc cgtttaaata tgctatatac gttgccgctg acagaacagc aacggttata
    18421 aagcttatca gtccgtctgc atggtgcctg gcatagcaat gaccgcttcc cggtatgatg
    18481 gcagaaagtg ttactgcaac atacggatta attttttctt tttgggctgc attcaatata
    18541 gtgtatatct ggtgcatctg ctgtagcctg tcgctatgtt ctgtataaac attcagtgca
    18601 ttgtcccagt catagcgatt gatatacacc gccaatatat tttgataata caacatttgt
    18661 aaaggtttat cctgcgctga tgtatcacac aaagaaagtg cctggagcgc atcctggtag
    18721 tgttgcagcg atgtatacac ttttgataaa agaaggtaat cgcagtattc ttttttttct
    18781 tttttttgca agatagcaac tgctgtagca agctggttgc ctttgcaata gcacagtgca
    18841 atggtgtaat ccatattgtc atctgtaagc atactatagc gtctggattc ggctatacac
    18901 tggtaataat tggccgtata atacagtgtg gaaattaatt gctgctgctt gtggacaaag
    18961 gcgtcatcag gatgtacaaa gaagagtgtc agtaacagca atgcgctcat ggatgctcag
    19021 ccagtgtttg tggcacggga tcatggtttg gtatggcgaa gggattgcag cgtataatcc
    19081 tgtctcccgc taaaaagcca cccatgacaa gtccatagcg cataactgcc tgtctgccgt
    19141 attcactgca tgttgggcgg tagcggcagt ttggtccatc ctggggggaa ataactacct
    19201 ggaacatccg gataaagaaa taacctattg ctccggctgc attgtgtatg gctggtgccg
    19261 attgcctccg ttcagtggtt ttttgtgcca cgctaacatc tgcatcccag ggagggaagt
    19321 cagcaaatgc cgagctgtta atgatgagcg acagtgttat aagcatgata gcacattgtt
    19381 tcatatactg taccccacaa tctaatagtt ctacatatag taacgcttct gctcggctac
    19441 ggtgctttta ggaaatcgtc cgtagtga
//
LOCUS       DAOSOX010000024        19136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2881_length_19136_cov_7.772496, whole genome shotgun sequence.
ACCESSION   DAOSOX010000024 DAOSOX010000000
VERSION     DAOSOX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2881_length_19136_cov_7.772496"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..370)
                     /locus_tag="PLX22_03245"
     CDS             complement(50..370)
                     /locus_tag="PLX22_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18952.1"
                     /translation="MNKKSTIIVTSIIIISFIIVVTIIVKGNRTSKHLNNKPVEDSKI
                     EISNENISKPEITLNPKNNANEYDIEYQSFKGIETDILLKNCYSLYSTYWSNEIKKYP
                     DHRN"
     gene            complement(561..1340)
                     /locus_tag="PLX22_03250"
     CDS             complement(561..1340)
                     /locus_tag="PLX22_03250"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007548066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOT18953.1"
                     /translation="MKVVLSKEQIEKVENNIRESLRNKFLTYKPETSNMPFHYRLLGR
                     DRMALFSFIHSLNTTFGTSIFEPVAEAVASLSFNLAQKQFVVGDTITELAQNEIQHIM
                     NGLTIGKGPNKIEEIERIRKVATSGRVNKIKTVKVDLFVKSHSGDIHFFDLKTAKPNI
                     SNFKDFKRTLLEWVAIYLFKHPEANVSSYIAIPYNPYEPKPYERWTLKGMLDLDNELK
                     VAEEFWDFLGGEGTYLELLDCFERIGIELRPEIDDYFSKFK"
     gene            complement(1337..2941)
                     /locus_tag="PLX22_03255"
     CDS             complement(1337..2941)
                     /locus_tag="PLX22_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT18954.1"
                     /translation="MSQLTLFKEEGIKLINLKEASAWASQYLNRKVTVSNISYLVQYG
                     KIKKYGYNGNIFVDIDELKNYYDSFQKQEDWMGKLGDDVNWHLSFVQYKEAERTKHVH
                     RLHPYKGKFIPQLVEYFLDSHVDEYKKEIYFHKGDIVLDPFCGSGTTLVQANELEMHA
                     IGIDISPFNCLISNVKVEKHNLILVEDIIHKLTSRLKDFQRTQNNAIFEEYLQLELTK
                     FNSEYFPSPEYKRKVSNGTINENKYAKEKEKEFIDVYNELIKKFKIKIIQNKNETFLD
                     KWFLEPVRKEIDFLSQEIKKIDNVDVKNVLTIILSRTVRSCRATTHADLATLKQPVTT
                     TYYCKKHGKICKPIFSITKWWEFYTIDTLNRFKDFDSLRTNTLQICLIGDSRTINLYE
                     EIKKKNTDLARILLKQKIRGIFSSPPYVGLIDYHEQHAYAYEIFGFERNDELEIGPLS
                     NGQGEVQRNSYSKSIAEVLLNGKKYLQNGYDIFLVANDKFNLYPKIAELAGMKIVNQF
                     KRPVLNRVEKDRGTPYAEIIFHLKER"
     gene            complement(3496..4083)
                     /locus_tag="PLX22_03260"
     CDS             complement(3496..4083)
                     /locus_tag="PLX22_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18955.1"
                     /translation="MVIKQNSRLLHWDYYLTLEEDLKNLARYIDINSNNFNTYSIELV
                     RILQIAASEIDVVLKLLCLKLNPGGNFVNISNYKNEIKSSWTEMINEKVFSSQYGLEL
                     TPWINWRGNSNPDWWRSYNNVKHKRNIYYHEANLKNVLNAVAALLICTSYYYLLDLTG
                     SKNLSQFKDAFLELGINSDFFKLNSEYYYYDLVVS"
     gene            4336..4650
                     /locus_tag="PLX22_03265"
     CDS             4336..4650
                     /locus_tag="PLX22_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18956.1"
                     /translation="MGLLKDASESLLNFSERFLDKTEELAQIARLTMEIKKLEHAINE
                     IYLSIGKHVYNSFESSNTINTDEFITGAIDTINNYKATIVEKQNEIQKVKELYESKHH
                     RY"
     gene            4664..6751
                     /gene="rnr"
                     /locus_tag="PLX22_03270"
     CDS             4664..6751
                     /gene="rnr"
                     /locus_tag="PLX22_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOT18957.1"
                     /translation="MSKTSSRIIQIIESQKGFFTPQDIVASLLFSKTKNKKSKKSNKP
                     PHTDIQKTADSIHNAIATLYHCGFLVKQKARFKVNHPFSFNGTFIHNKKGDGIVYFND
                     TEVHIYREDTNHARNKDIVQVKITDIRRGTLLGTVTTIVSRNKQMFFARCLKPYGKFN
                     ILQLLDVPGNIQVITSEKIKQGMLAIVTVKNTFISNYAECTINTVIADEEQHDVERIV
                     LKHSLPGAHPEYPHLHAIENYVKPHELASRTDYRQLLTVTIDGETAKDFDDAISLEYR
                     RGTYTLYVHIADVSAYVYSNDDIDKEAYARGTSYYLGNTVIPMLPERLSNDLCSLKAG
                     TDRLTLSVMIQFNEQGQYKKHHITRGIINVDRRLTYTQAQEIIHSSKRTPIKTMLMHA
                     KDLSDKLKNLRLSHGRVDLNLADAEMIFDIDSSITDIAFATRLDSHRIIEECMLSANE
                     VVSRILREKNIPALYRIHEPIAEEKLVALTNFLKTLGITLHQEENIGLALQKVIDSVS
                     QKEYEQVVNFIVLKSFMQAYYGSKPLGHFGLGFKDYTHFTSPIRRYPDLIVHRCIKSL
                     IDALPPPYLPDELENIGLQSSKLERVAQSAERDLSKLKACRLMHDKIGKQFEGIISGV
                     TRFGFYVSLLDKPIEGMVPLKFLTDDYYLVNEDEYTIIGRRLGRRFRLGDTITVKLVG
                     VDIDLMRIDFEVV"
     gene            6752..8221
                     /locus_tag="PLX22_03275"
     CDS             6752..8221
                     /locus_tag="PLX22_03275"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOT18958.1"
                     /translation="MVNLQAKGFDALATSDTVALVCTQEQVASNAFIPEQLHDVFALI
                     DIKTFKAKPNSYYYNALKNKPAILLVGLGPQNNVSAETLRNAASTIVQACQDKGLTQI
                     HIVVPSLTAVPDGARCIAEGVFLSNYNFNRYKTNNDESAPLLQKAILVCDNPQQLSPM
                     LKEVAVVCSNTLLCRDLVNETTENANPVTIAKLAKKIAAQHAIQCTVYTKKDITKMKM
                     GLLLAVSRGSIYPPYCIVMRYRGAPSKKESIAIVGKGITFDTGGINLKPTGHIETMRT
                     DMAGAATCIYAIAAVAQMKLLMNVYVVIPLAENMISHTAYKPGDVFTAYNGKTVEILS
                     TDAEGRLILADALSFTEKTIKPKAIIDLATLTGACIVSFGEIVAAYLTNDEQLSTALI
                     KASEATGEKIWRLPLYKEYSEEMKSDIADIVNVPAQRNAGTIMGATFLKNFIEDTPWV
                     HIDIAGTAWYSKKRGYVPKYATGFGVRLLYRFVKDISGQ"
     gene            8493..8966
                     /locus_tag="PLX22_03280"
     CDS             8493..8966
                     /locus_tag="PLX22_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT18959.1"
                     /translation="MYSTKELIDIAMGIEETGYYFYSTLKSKFKDESFQQMFQFLADE
                     ELRHKDIFSNMLKGLNDTQGVFTEEYYTYLKAIGTTRVFSNSNDVDTIAPTLNQPLDI
                     IKIAMDAERDSIVWYSELKEIYRNDTRSKDLLERLINEERKHVLILLDLKEKLSM"
     gene            9328..11244
                     /locus_tag="PLX22_03285"
     CDS             9328..11244
                     /locus_tag="PLX22_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HOT18960.1"
                     /translation="MEKGDQQNMSKYQEAYDESINNPEKFWGEAAKGIHWYKPYTRVL
                     DDSKKPFYRWFVGGELNTCYNAIDRHVDSGNGSRIALIYDSPVTGTIAHYSYKELLDK
                     VSLFAGALQKYGIKKGDRVIIYMPMIPEALYAMLACARIGAIHSVVFGGFAPNELAIR
                     IDDAKPKLIVSASCGVEGKKVIEYKPLLDKAIEIANHKPDHCIIFQRPQVKAPLEHGG
                     RDIDYNDALSDAKPADCVPVASTDPLYILYTSGTTGIPKGVVRDNGGHAVVLNWSMKN
                     IYNIQPGDVWWAASDVGWVVGHSYIVYAPLLYGATTILYEGKPVGTPDPGAFWRVISQ
                     HKVKALFTAPTAFRAIKKEDPNGEYFKKYDLSQFNYLFLAGERLDPDTYYWATDLLKK
                     PVIDHWWQTETGWPIAANCVGIELLPIKAGSPTKPVPGYQVQIINEEATVLPNGTEGI
                     VVIKLPLPPGTLLTLWQNDEKFKESYLDIFDGYYFTGDGGYFDEDGYLYIMGRIDDVI
                     NVAGHRLSTGAMEEVISKHPDVAECAVIGAEDSFKGQLPVGFVVLKEGVKKSKEQITS
                     ELAQMVRDQIGAVACYKTTLVVQRLPKTRSGKILRGTMRKIADNKPFAIPSTIDDPSI
                     LDEIKDALSTISIS"
     gene            complement(11292..>12553)
                     /locus_tag="PLX22_03290"
     CDS             complement(11292..>12553)
                     /locus_tag="PLX22_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT18961.1"
                     /translation="FYGEFVLFPEAIDEELAKRANELHDVDVRSVCYMRVPKIVEADP
                     EREHFILNDFHFGTISRRLYDQHLCNYIPMTYHQGPRVTKKYLDYDIVFLTVGPMDPR
                     GYFNYGLANSVTSAVVTKAKKIVVEVNEHVPYCLGGNQESIHISRIDHIVEGKNLPLP
                     QIPALEPTEIDKQIAKHLLAEIEDGSCLQLGIGGLPNIVGQMIAEGDFKDLGVHTEML
                     VDSYVDLYQAGRVTGSRKNIDKYKMSYTFAMGTDKLYDFLHHNPTCASYPVNYINDPR
                     IIALNDKVVAVNNAIEVDLFSQVSSESVGIIHKSGTGGQLDFIFGAFGSHGGKGFICL
                     SSTYHDKHGNIVSRIVPTLPQGAIVTVPRSIVHYVVTEYGAVQLKGKSTWERAEALIS
                     IAHPDFRDELIEKATEMNIWTRTNKLV"
     assembly_gap    12554..12653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13214..13423
                     /locus_tag="PLX22_03295"
     CDS             13214..13423
                     /locus_tag="PLX22_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18962.1"
                     /translation="MTFQKNSGLCYCIKYIGNYPIDVNNRIMPMNDTLFNTYYQKRYM
                     VLAAIMLDSKTFLIPINVTKGLTYL"
     gene            13738..14565
                     /locus_tag="PLX22_03300"
     CDS             13738..14565
                     /locus_tag="PLX22_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT18963.1"
                     /translation="MIEIKVLLENTSISSNFTPKHGLSIAIYTGSSNILLDVGPDKTF
                     YENANKMQCNIEQIETLILSHSHIDHAGGLNKFVEVNKRAEILLFDKYDSKYFTKIIG
                     PLKISVGIKCNDAATKRIKTISSNQQIDEKTTFIRNNSTHYKKPTFNKTLYKAIGKNY
                     TLDNFEHEGILVIDDGELVIFNSCSHNGIYNSIASVKNIFKGKKIRSYIGGLHYTNPF
                     TKQHENTIELDTLIDFVKTEGIKLYSGHCTGKYCIKYLKKELGDKFVTIATGSELFV"
     gene            complement(14621..>14916)
                     /locus_tag="PLX22_03305"
     CDS             complement(14621..>14916)
                     /locus_tag="PLX22_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18964.1"
                     /translation="LQLDTNKVKEILYTFDPLKAYSFPQVDTAMLKGKIASLTVAVQN
                     ENNEKAAEEISLTVHKEYENNRYICTCSKTPYVFTIDEWRAKRIFKTLQDLKK"
     assembly_gap    14917..15016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15017..15530)
                     /locus_tag="PLX22_03310"
     CDS             complement(<15017..15530)
                     /locus_tag="PLX22_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOT18965.1"
                     /translation="MQVKKNIVISIVVIVVLGGYLLLNKFSSRELLQLPEVSCAISQI
                     VIAKDGNSITIIKDGDSWKVNGFEADKNVIANIENKLKDIEVVDFVSEKPLYERFELD
                     PEHAIHVVAKCGDTVVRDFYVGKKGSTYRHTYVRFEGNKAVYLAADTLDTYFNKTVDD
                     LRNKDIVQLDI"
     gene            complement(15553..17697)
                     /locus_tag="PLX22_03315"
     CDS             complement(15553..17697)
                     /locus_tag="PLX22_03315"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOT18966.1"
                     /translation="MNMGNIKKYSNKIVDRFDAALRRIMQKIGKAPDKPQKQKSYSHF
                     ILYGIIIILINLVGLTLYVRLDLTKNSVYSLSPISKAVVSTLEEPLTVKIFFNKDLPA
                     PYNTVYRYLQDLMVEYDNAGNRYFHYEFIDVEKQKDAAGDFGIYPVQIREIRNDQVKF
                     RNAYMGLAIIHADIIEKIDSITEPEGLEYRITTLIKKMNGKIDALLKIKDPITVTLYA
                     SSNLPIPGMQNLNEQVYAQVQKCNIRNYNKIQYRFVDPVNNPQALTIAQMYGLPTLKW
                     PQFTTMEGRAIPPGQGMVGIVVEHNNKFETVQILTRSIFGQYAIGDLSRLEDMLNAAI
                     DNLISVNPKVCYITGHGEKDINDEQNGAAQFRKMLSDMYDLVTVDITKDEIPDDVITI
                     IINGPRSMYSEYELFKIDQFLMRGGNAILLLDSYVEQQAEGMQMFQRPPQALPVITGL
                     EGMLAHYGIKIRKDIVMDKQCFIAQQRGLGELTIYFAPMIDDEGLSDDCEITKYLKKI
                     VFLKASSLEADEDLLKANKASVKYIVKSSPYSWLQQGAINFMPWGVNPPADNNLASYN
                     LAILVQGNLKSYFTDKMPTIDAKGKNAKSQDYIQQEVIKETVKPVKLFVAGTSEITGR
                     NIIDDEGKSPNAVFMHNVVDYMQGNYDIPQMRSKGLSFNPIKETSDTAKSIIKGFNIA
                     GLPILVLIAGAVYMWRRKLRRKHIESQYTMMR"
     gene            complement(17701..18426)
                     /locus_tag="PLX22_03320"
     CDS             complement(17701..18426)
                     /locus_tag="PLX22_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT18967.1"
                     /translation="MQQFKDSMKGAQLVAKKELAGFFSTPIGYIVISVFLVIAGFLFF
                     STFFLYGQAELRAYFELLPLLFAFVIPAITMRLFAEERNTGSFEMLMTLPLSTVQIVT
                     GKILAATIFAGIMVLPTLFYAVSVAFVGSPDIGPIIGGFIGAIILAAAYASIGVFASS
                     LTRNQIVAFIIGFAICIVLSLITKFLYFVSPKLVSLFEYISADYHFQNIARGIIDTRN
                     IIYFASVIIISSMASIYQLEVRR"
     gene            complement(18439..>19136)
                     /locus_tag="PLX22_03325"
     CDS             complement(18439..>19136)
                     /locus_tag="PLX22_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT18968.1"
                     /translation="YLPESAPLYPDMMVYDFLQFIADIREINDVERITDVAIQCGITE
                     VMHKNINELSKGYKQRVGLAQAILHDPEILILDEPTNGLDPNQIIEIRDLIKELGREK
                     TVILSTHILQEVEATCSRVIIIDKGVIVADDLTSELKSAKGKDIRINILAGGASFDQL
                     KTALCAIPGINNVNHIHDEEGLCHVQVITGSDIDARPAIFNCIKDNGWVLYEMNREYR
                     SLEHVFRELTVGA"
BASE COUNT         5991 a         3624 c         3232 g         6089 t
ORIGIN      
        1 ctcttatttt tctattgcaa gcattttttt aaagatttgt tcagaggtct caatttcgat
       61 ggtccgggta cttttttatc tcatttgacc aataggttga atataatgaa tagcaatttt
      121 ttaatagaat atcagtttca atgcctttga acgattgata ttcaatatca tattcgttag
      181 cattattttt tggattaaga gtaatttcag gctttgaaat attttcattt gaaatttcaa
      241 tttttgaatc ttctacaggc ttattattta aatgcttaga agttctattt cctttaacaa
      301 ttattgtaac aactattatg aaagagatta ttataataga agttacgatt atagttgatt
      361 tcttattcat ctttttatgt tactccataa aatttttacg tggtttccta taacgtatca
      421 gcgtagccga tgttgttcca ctattatcaa tgtgccaaag gccaagcact gtaggcaacg
      481 tcgttgccga aaaacacagc ggtttatgct gcgttatgcg ctgatggtcg tttttggtca
      541 tcgacaaaat tctcatatat ttatttgaat tttgaaaaat aatcatctat ctctggtctt
      601 aattctattc ctattctttc aaagcaatcg agcagttcta aataagttcc ttccccgccc
      661 agaaaatccc aaaattcttc tgcaaccttt aattcattat ctaaatccag cattcctttt
      721 aacgtccatc gttcataggg ttttggctca taaggattgt aaggaatagc aatataagaa
      781 gatacatttg cttctgggtg tttaaataaa taaattgcta cccattctaa tagcgttctc
      841 ttaaaatcct tgaaattgct aatattgggt ttagctgttt ttaaatcaaa aaaatggata
      901 tccccactat gacttttaac aaataagtcc acctttactg tttttatttt atttactctg
      961 ccagaagttg ctacttttct tatcctttca atttcctcta ttttgtttgg tccttttcca
     1021 attgtaagcc cattcattat atgttgtatt tcgttttgag ccagttctgt tattgtgtca
     1081 cctacaacaa attgcttttg tgccaggtta aaactcaaac ttgccactgc ctcagctacg
     1141 ggttcaaaaa tagaggtgcc aaaagtagta tttaaagaat ggataaaaga aaataatgcc
     1201 attctatctc tacctaacaa acgataatga aaaggcatat tacttgtttc cggtttatat
     1261 gttaaaaatt tatttcttaa actttctcta atattatttt caactttttc tatttgttct
     1321 tttgaaagta ccactttcat ctttccttta aatgaaatat tatttctgcg taaggagtgc
     1381 ctctgtcttt ttcaactcta ttaagaacag gtctcttgaa ttgattaaca attttcattc
     1441 cagccaattc tgcaatttta gggtaaagat taaatttatc gttagcaact aaaaaaatat
     1501 cataaccatt ctggagatat tttttaccat ttaatagaac ttctgctata cttttactat
     1561 aagaatttct ttgaacctct ccctgtccat ttgataaagg tcctatttct aattcatcat
     1621 tacgttcaaa tccaaatatc tcatacgcat aagcatgttg ttcgtgataa tcaataagtc
     1681 caacataagg tggacttgaa aatattcctc ttatcttctg ttttaacaat atcctcgcaa
     1741 gatctgtatt tttctttttt atttcttcat ataagtttat tgttctacta tcaccgatta
     1801 gacaaatttg taatgtattg gtccttaaac tatcaaaatc tttaaaccta tttagtgtat
     1861 ctatagtgta aaattcccac cattttgtga tagaaaatat tggtttacag atttttccat
     1921 gttttttgca ataatatgta gtggtgacag gttgttttaa agtagctaaa tcagcatgtg
     1981 tcgttgctct acaggaacga acggttctac ttaatattat ggttagtaca ttttttacat
     2041 ctacattatc tatttttttt atttcttgtg ataaaaaatc tatttccttt cttacaggtt
     2101 ctaagaacca cttatctaaa aaagtttcat tcttgttctg tattattttt attttaaatt
     2161 ttttaatcaa ttcattataa acatcaataa attctttttc tttttccttt gcgtatttat
     2221 tctcattgat agttccattt gaaacttttc ttttatattc tggtgaaggg aaatattcag
     2281 agttaaattt tgtaagttct aactgtaaat attcttcaaa aatggcatta ttctgtgttc
     2341 tttgaaagtc ttttaatcgt gaggttaact tatgtattat atcttcaact aaaattaaat
     2401 tatgcttttc aacttttaca ttagaaatca agcaattaaa aggagaaata tctattccta
     2461 tggcatgcat ttctaattca tttgcttgaa ctaatgttgt tccacttccg caaaatggat
     2521 ctaatacaat atcgccttta tgaaagtaaa tctctttttt atactcatct acatgagaat
     2581 caagaaaata ttcaactaat tgagggataa acttaccttt gtaaggatgt aatctgtgaa
     2641 catgcttagt cctttctgct tctttatatt gaacaaatga tagatgccaa ttaacatcat
     2701 ctcctaattt tcccatccaa tcttcctgtt tttgaaaaga atcgtaatag ttttttaatt
     2761 catcaatatc tacaaaaata tttccattat agccatattt ttttattttt ccatactgaa
     2821 ctaaatatga tatatttgag actgtaactt tcctattcag atattgagaa gcccaagcac
     2881 ttgcttcttt taaattaatc aatttgatac cttcttcttt aaataatgta agttggctca
     2941 ttacttaact tcccaatttt tgtatttttt aattaaatca tagtaaatat tttttccatt
     3001 tccaggcgat caatggcgta taactccatc atatcctaac tactttgcct atcccacttt
     3061 cactcgttgt tattgtccaa taatgcatta caatctcaat catcagaaga gctgtgcacc
     3121 ttatatacta aaacaaccgc cttcctgcac aacatctact ctgtgttttt catcgctttt
     3181 taaatcaata tatatgttat gatatattat acaccataat catttaaaaa agttaccatt
     3241 gtcaagatta ttttactttc aatacagcgt gcacggaggc agatgcatgg taagttgggc
     3301 tgataatggc aacattttgt ttatacaatg tcacggcggt ctttcgatgc gatatggtgt
     3361 agcccaaaca gccgtcaagt agtgaagcgc ataagctttg ttattcggag tcggaccgcc
     3421 atgattgcaa tattttgccg tagccatgga caacggaggc acaacaacta ttttgttatc
     3481 ttatcaatct taatttcatg aaacaacaag atcatagtaa tagtattcac tatttaattt
     3541 aaagaaatca gaattaatcc caagctccaa aaaggcatct ttgaactgtg ataaattctt
     3601 tgaaccagtt aaatcaagca aataataata actagtacaa atcaataaag cagctactgc
     3661 atttaataca ttttttaaat ttgcttcatg gtaataaata ttacgtttat gttttacatt
     3721 attataagaa cgccaccaat ctggattcga attacctctc cagtttatcc acggagttaa
     3781 ttcaagtcca tattgagatg aaaatacttt ttcattaatc atttcagtcc atgagctttt
     3841 aatttcattt ttatagtttg agatatttac aaaatttcca ccaggattca attttaaaca
     3901 taataatttt aaaactacat caatttcaga tgcagcaatt tgaagaattc gaaccaattc
     3961 aatagaatac gtattaaaat tattagaatt aatatcaata tatcgcgcta aattttttaa
     4021 atcttcttca agtgttaaat agtagtccca atgtaataat cttgaatttt gtttaatcac
     4081 catccattat ctccagatta ttatatattc agcaataatc ttcatttagt agtcactgaa
     4141 gtcgggcgcc ttgttaagtt aggctggcta tttcctataa ccccttcaca tccttatttt
     4201 ttcatatatc tcatccattg tcaagattat ttcagaaaca atggggtcag agcctcaatt
     4261 attcaaaata tattgacatt cttttacaca tgttatatat tgaagcttta atgttacatc
     4321 tacaacgagg ttactatggg tttattaaaa gatgccagcg aatcattgtt aaatttcagc
     4381 gagcggtttt tagataaaac agaagagctg gcgcagattg caaggcttac catggaaata
     4441 aaaaaattgg aacatgccat aaacgagata tacttatcta ttggcaagca tgtgtacaac
     4501 tcatttgaaa gcagcaacac tataaatact gatgaattta taacaggagc tatcgacaca
     4561 ataaataatt ataaagcaac catagtggaa aaacaaaatg aaatacaaaa agtcaaggaa
     4621 ctatatgaat caaaacacca tcgttattaa aactttatag catatgtcta aaacttcatc
     4681 gcgcattata cagattattg aaagtcaaaa gggttttttt acacctcagg atattgtagc
     4741 ttctttgctg ttttcaaaaa caaaaaataa aaaatcaaaa aaaagcaata agcctccgca
     4801 tactgacatt caaaaaactg ctgacagcat ccacaatgct atagcaacat tataccattg
     4861 cggttttctt gtaaagcaaa aagccaggtt taaggttaac caccccttta gcttcaacgg
     4921 tacatttatc cataacaaaa aaggtgacgg cattgtgtac tttaatgata ccgaggtgca
     4981 catctaccgc gaggatacaa accatgctcg caacaaagat attgtccagg ttaagattac
     5041 cgacatacga cgtggcacac tgcttggcac tgttaccaca attgtttcgc gcaacaagca
     5101 gatgttcttt gcccgctgtt taaagccgta tggcaaattc aacattttgc agcttctgga
     5161 tgttccgggc aacatacagg taataaccag tgaaaaaata aaacagggaa tgctggcgat
     5221 agttactgtt aaaaatacct ttatcagtaa ttatgctgaa tgcaccatca atacagtcat
     5281 tgccgacgaa gagcagcatg atgtagagcg cattgtgttg aagcattcgt tgccgggagc
     5341 tcatcctgag tatccccatt tgcatgcaat tgaaaactat gtaaagcccc atgagcttgc
     5401 cagccgcacg gactaccgac aattactaac cgttactata gacggcgaaa ctgcaaaaga
     5461 ttttgatgat gccatatcgc tggaatacag gcgtggaaca tacacattat atgttcacat
     5521 tgccgatgta tcagcctatg tttatagcaa tgatgacatt gataaagagg cgtatgcacg
     5581 gggtacaagc tattatctgg gcaatactgt cattcccatg ctgcctgaac ggctatcaaa
     5641 tgatctgtgc tcgcttaaag ctggtacgga taggttaacg ctttcggtca tgattcaatt
     5701 taatgaacag ggacaatata aaaagcatca tataactcgg ggtataatca atgttgaccg
     5761 gcgtttaacc tatacccagg cgcaggaaat tatccattca tcaaagcgca ctccaatcaa
     5821 aacaatgttg atgcatgcaa aagatttatc ggataaactt aaaaacttac ggctttctca
     5881 cggaagggtt gatcttaatt tagctgatgc tgagatgata tttgacatcg attcctccat
     5941 taccgacata gcatttgcaa cacggcttga tagccaccgt atcattgagg aatgtatgtt
     6001 aagcgctaat gaggttgtat cacgcatctt gcgcgaaaaa aatatccctg cactgtaccg
     6061 tatacacgaa cccatagcag aagaaaaatt ggtagcgtta acaaacttcc tcaaaacact
     6121 gggaataacc ttacatcagg aagaaaacat tgggcttgca ctgcaaaagg taattgattc
     6181 agtatcccaa aaagaatatg agcaggttgt taattttata gttctaaaat cgtttatgca
     6241 ggcatactat ggatcaaaac ctctggggca ttttgggctt ggctttaagg attacactca
     6301 ctttacctcg cccatcaggc gatatcctga tttgattgta catcgctgta tcaaatccct
     6361 gatagatgca ttgccgccgc cctacctgcc cgatgagctt gaaaacattg gcttgcaatc
     6421 atcaaaatta gaacgagtgg cacaatccgc cgaacgtgat ctttctaagc ttaaagcatg
     6481 caggttgatg catgataaaa ttggcaagca gtttgaaggt attataagcg gcgtcacccg
     6541 ttttggattt tatgtaagtt tgcttgacaa accaatagag ggtatggttc cgttaaaatt
     6601 ccttaccgat gactattacc ttgtcaatga agatgagtac acaatcattg gcaggcggct
     6661 gggacgtcga tttagattgg gcgataccat taccgttaag cttgttggtg ttgatattga
     6721 ccttatgcgc atcgattttg aggtggtgtg aatggtaaat ctacaggcaa aaggttttga
     6781 tgcattagca acatccgaca ccgtggcgtt ggtatgtaca caggagcagg ttgccagcaa
     6841 tgcatttatt ccagaacaac tgcacgatgt ttttgcactg attgatatca aaacgtttaa
     6901 ggcaaaacca aattcgtact attacaatgc attaaaaaac aaaccagcaa tactccttgt
     6961 tgggctgggc cctcaaaaca atgtaagtgc tgaaaccctg cgaaacgcag caagcaccat
     7021 cgtacaggca tgccaggata agggtttaac gcaaattcat atcgttgttc cctctttgac
     7081 tgctgttccc gatggtgcac gctgtattgc tgaaggtgta tttctttcaa attacaattt
     7141 caatcggtat aaaacaaata atgacgaatc tgctccgctg ctgcagaagg ctattctggt
     7201 gtgcgataat ccacaacaac tatcgcccat gctgaaagag gttgccgttg tatgcagcaa
     7261 tacactttta tgccgtgacc ttgttaatga aacaacagaa aatgctaatc cagtaactat
     7321 cgccaaactt gcaaaaaaaa ttgcagcaca gcatgccata caatgtaccg tatacacaaa
     7381 aaaagatatt acaaagatga agatgggact gcttcttgcc gttagccgtg gaagcatata
     7441 tcctccctat tgtattgtta tgcgttacag aggtgcacct tcaaaaaaag aatctattgc
     7501 catagttggg aaaggcataa cctttgacac cggcggcatc aacctgaagc caacagggca
     7561 tattgaaacc atgcgaaccg atatggcagg agcagcaacc tgtatctatg ccatcgcggc
     7621 agttgctcag atgaagcttc tcatgaatgt gtacgtggtg ataccgttag ccgaaaatat
     7681 gatatcgcat actgcgtata aacccggtga tgtgtttacc gcatataacg gcaaaaccgt
     7741 tgagatttta agcaccgatg cggaagggcg ccttatttta gccgacgctc tgtcatttac
     7801 cgaaaagact attaaaccaa aagctatcat tgatttagct acacttaccg gcgcctgcat
     7861 agtaagcttt ggtgagatag tagctgcgta tttaaccaat gacgaacaat tatcaaccgc
     7921 attaatcaaa gcttctgaag ctaccggcga aaagatatgg cgcctgcctt tgtataaaga
     7981 atacagtgaa gagatgaaaa gtgacatcgc agatattgtg aatgtgcctg cacagcgcaa
     8041 tgcaggtact atcatgggtg caacattctt aaaaaatttt attgaagata ccccatgggt
     8101 gcatattgat atagccggaa ccgcatggta ttccaaaaaa cgcggatatg ttccaaaata
     8161 cgccaccggg tttggtgtgc gtttattgta caggtttgtt aaggatatat ccgggcaata
     8221 acactatgct gtcatagtag tcatcctgaa cgtagaatgt catcctgaac ttgtttcagg
     8281 atctcatcta atagtgagat gcagaatcaa tttcagcatg acaactgtta atgcaaggtg
     8341 gagctaaccc catcgactta tcaaggaaag gagttggggg tgaggttaaa acatttcaag
     8401 ttttcatcaa gcaatacaaa aagtgtattg accaaattta cagcatagtt aaggatgatg
     8461 tgtttattat ttaataatct ctggaggaac tcatgtactc aacaaaggaa cttattgata
     8521 tagcaatggg cattgaggaa accggatatt atttttattc aacattaaaa agcaagttta
     8581 aagatgaaag ttttcagcag atgtttcaat tccttgctga tgaggaattg cgtcataaag
     8641 atattttcag caacatgctc aaaggattaa acgataccca gggcgtattt accgaagagt
     8701 actatacata tcttaaagct attggcacaa cacgagtatt ttcaaatagc aatgatgttg
     8761 atacaatagc accaacgctc aatcaaccgc ttgacattat caaaatagca atggatgccg
     8821 agcgtgactc cattgtatgg tattcagaat taaaagaaat ctaccgtaat gataccagat
     8881 caaaagacct attagaacgt ttaatcaatg aagagcgaaa gcatgtgctt atcctgcttg
     8941 atttgaagga aaaattaagc atgtagccgt ttttaccctt acattggagt tatatatata
     9001 gttaccttgt attgcttccc tgattgattc agattatgtc aattttttat ttgtttgttt
     9061 gctattcgtt tttaattttt aaaacttgag taaaaggaaa cagcaatttt cttttatgta
     9121 atactacata tattaatcat tgacaaatat ttttgcaaaa atatcctgtc aatttaacct
     9181 gcatatattt ctttattgtg tgctgttgta tcacgctact gtataattac aaatagtata
     9241 tcatggtaac attgtaaaat ttcattgagc tactgtattg cataagtgtt agcagatatt
     9301 ggtaagtaaa atctatatac aaaatccata gaaaaggggg atcagcaaaa catgagtaag
     9361 tatcaagagg cgtatgatga atcaatcaat aatcctgaaa aattctgggg tgaagctgca
     9421 aaggggattc actggtataa accatatacc agggttttag atgattcaaa aaagccattt
     9481 taccggtggt ttgtcggcgg tgagcttaat acctgttaca atgctattga ccgtcatgtg
     9541 gacagcggta acggcagccg tatagcttta atctatgata gtcctgtaac aggaactatc
     9601 gcacattata gttataaaga attactggat aaggtatcat tgtttgccgg cgctttgcaa
     9661 aaatatggca ttaaaaaagg ggatagggta atcatctata tgccaatgat ccccgaagct
     9721 ctttatgcaa tgctggcatg tgcacgcatt ggtgcaatac attccgttgt ttttggcgga
     9781 tttgctccta acgaacttgc aatacgcatt gatgatgcaa aacccaaatt aattgtgtct
     9841 gcaagctgtg gcgttgaagg caaaaaagta attgaataca aaccattgtt agacaaagcc
     9901 atagaaattg caaatcacaa acccgatcat tgtattatat ttcagcgtcc gcaggtaaaa
     9961 gcaccactgg aacacggagg cagagatatc gactacaacg atgcgttatc agatgcaaaa
    10021 ccagcagatt gtgtaccggt agcatctacc gacccgcttt atattttata tacttctggt
    10081 accactggta ttccaaaagg ggtggtaagg gacaatggtg gtcatgctgt agttctcaat
    10141 tggagcatga aaaatatata caacattcaa cccggtgatg tatggtgggc agcttctgat
    10201 gttggctggg ttgtgggaca ttcatatatt gtttatgcac ctcttttata tggtgcaaca
    10261 acaatactgt atgagggaaa accggttggc actcctgacc ctggtgcgtt ctggcgcgta
    10321 atttcacaac ataaggttaa agcgctgttt accgctccca ctgcattcag agccattaaa
    10381 aaagaggacc ctaacggcga atattttaaa aagtatgacc tgtcacaatt taactatcta
    10441 tttttagccg gggagcgttt agatcctgat acctattact gggcgacaga tctcttgaaa
    10501 aaacctgtta ttgaccactg gtggcaaacc gaaacaggct ggcctattgc tgctaactgt
    10561 gttggcattg agctgttgcc cattaaagca ggttcaccca ccaaaccggt tcccggctac
    10621 caggttcaga tcatcaacga agaggcaacc gtgttgccaa acggtacaga agggattgtt
    10681 gttatcaaac ttccgctacc acccggaaca cttctaaccc tgtggcaaaa tgatgaaaaa
    10741 tttaaagagt catatttaga tatttttgac ggctattact ttaccggtga tggcggctat
    10801 tttgatgaag atggttatct gtatataatg ggtcgtattg atgatgtaat caacgttgca
    10861 ggacaccgac tttcaactgg cgccatggaa gaggtaattt ccaaacatcc tgatgttgcc
    10921 gaatgtgcgg ttattggtgc agaggatagc ttcaaaggac aactgcccgt tgggtttgta
    10981 gtattaaaag aaggtgtgaa aaaaagcaaa gagcagataa catctgaatt agcacagatg
    11041 gtacgagacc agattggtgc tgttgcatgt tataaaacca cactggttgt acagcgcctg
    11101 cctaaaactc gctcgggtaa gatattacgc ggaacaatgc gtaaaattgc tgataataag
    11161 ccatttgcca taccatccac gatagatgat ccgtcaatcc tggatgagat taaagacgct
    11221 ctatcaacga taagtattag ttaataaaaa gtaatatcaa tcgcatcatc aattacggtg
    11281 atgcgattgt gttataccag cttattggtt ctggtccata tattcatttc agttgctttt
    11341 tcaattaatt catctctaaa gtcaggatga gcaatgctta taagtgcttc tgctcgttcc
    11401 catgttgatt ttccttttaa ttgcactgct ccatattcgg taaccacata gtgtactatg
    11461 gaccgtggaa cagtaactat cgccccctgg ggcaatgtag gtacaattcg tgaaactata
    11521 ttcccatgtt tatcatgata ggtagatgac aaacaaataa atcccttgcc gccatgtgaa
    11581 ccaaatgcac caaatataaa atcaagctgg ccacccgtac ccgacttgtg aattatcccc
    11641 accgattcag aagatacctg actgaacagg tcaacctcaa tggcattgtt aaccgctact
    11701 actttatcat taagtgcaat aatacgtgga tcatttatat aattgacagg gtaggaagca
    11761 catgtggggt tatgatgcaa aaagtcatat aatttatcag tacccattgc aaaggtatag
    11821 ctcattttat atttatcgat atttttccgg gaaccggtaa cccttccagc ctgatataaa
    11881 tcaacgtaag aatcaacaag catctcggta tgaacaccta aatccttgaa atcaccttcg
    11941 gcaatcatct gacctactat attaggcaat ccgcctattc ctaactgcaa acatgaacca
    12001 tcttcaattt cggccagaag atgctttgca atctgtttat caatttctgt tggctccaat
    12061 gcaggtatct ggggcaacgg taaatttttt ccttccacaa tatggtcaat acgtgatata
    12121 tgaattgatt cctgattgcc acccagacaa tacggaacat gctcattaac ttccacaaca
    12181 attttttttg cttttgttac aacagccgat gttactgagt ttgccagacc ataattaaaa
    12241 taaccacgcg ggtccatggg gcctaccgtt aaaaaaacaa tatcataatc aagatatttt
    12301 ttggttaccc gcggtccctg atgataggtc atgggtatgt agttacaaag atgctgatca
    12361 tacaatcttc gtgagatagt tccaaaatga aagtcattta atataaaatg ttctcgctct
    12421 gggtcagctt cgacaatttt tggaacacgc atgtaacata cgctacggac atcaacatca
    12481 tgcaattcgt tagctcgttt tgcaagctct tcatcaatag cttcaggaaa caaaacaaat
    12541 tcaccataaa aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttgcaa
    12661 gctcttcatc aatagcttca ggaaacaaaa caaattcacc ataaaaaacc ctgtaacccg
    12721 atttaacaaa acgtgctgcc tcatctgcag ttatcttttt ttgatcatat tcccattttt
    12781 caatatcacc cattttgcca cttccctatc gatataatct tattattgta tgactacata
    12841 gtattttata ttattgatta ttataataca acatcaatgc tctatattga gtatagcatt
    12901 aacaaattta caattcaaat tattatatat tattttatac tatattttca tttttgttga
    12961 ttaaatacca actggttagt ttatataaat tatattaatt gtaagcactg actgtatgaa
    13021 aataatgaaa catatttttt acattgttat gttgagtaag gtataattct ttgttattct
    13081 atggtagtgt cactttttgt catttttaaa agaatgtaat gactgaagta tctatgtttt
    13141 tataaaatga catgattttt cactttgttc agaatgacta tcgactttac tcattatagg
    13201 catattctgc aaaatgacat ttcagaaaaa tagtggactt tgttactgta ttaagtatat
    13261 cggcaactat ccaattgatg tcaacaacag gataatgcca atgaatgata cattgttcaa
    13321 cacctattat caaaaacgct atatggtcct tgctgcgatt atgctggata gtaaaacttt
    13381 tttaatacct ataaacgtta caaagggttt gacctattta taaatttata gcaataaaaa
    13441 gttttataat agggcatgat attcttgtga tgaagaatat attttgataa agattatttt
    13501 ttcaaggtta cgttgccata tactttaaaa atagactgaa aaaatattga cagtgttagt
    13561 attgcagtta ccattaataa tacaccattt gcttattatc ctatactatt caggagaata
    13621 gcaaaccaca tttataagga agatggggaa aataggcttt tcatgttcat cgatatatga
    13681 ctaaaccata ttgttttcaa cggacaggca tatataattc aaataaaagt gatatctatg
    13741 atagaaatta aagtgctatt ggaaaatact tcaatatcaa gtaattttac gccaaaacat
    13801 ggattgtcaa ttgcaatata tactggaagt tccaatattt tacttgatgt aggacctgat
    13861 aaaacgttct atgaaaatgc taacaaaatg caatgtaata tagagcagat agaaactcta
    13921 atcttatctc attcacatat agaccatgca ggtggcttga ataaatttgt tgaggtaaat
    13981 aaaagggccg aaattttgct gtttgataaa tatgactcta aatattttac aaaaatcata
    14041 ggcccactta aaatatccgt tggaattaaa tgtaatgatg ctgctacaaa acggataaaa
    14101 acaatatcaa gcaatcagca gatagatgaa aaaactactt ttatcagaaa taatagtact
    14161 cactataaaa aacctacttt taataaaact ttatataaag caataggaaa aaactacacg
    14221 ctagataact ttgaacatga aggaatactc gttatagacg atggggaatt ggttattttc
    14281 aactcgtgtt ctcataacgg gatttataac agtattgcaa gtgtaaaaaa tatatttaaa
    14341 ggtaaaaaga taaggagcta tattggagga ttgcattata ccaatccatt tacaaagcaa
    14401 catgagaaca ctattgaact tgatacgtta atagattttg taaaaactga aggaataaag
    14461 ctgtattcag ggcattgtac cggtaaatat tgtataaaat atttgaaaaa agagcttggg
    14521 gataaatttg ttactattgc gactggatct gaattatttg tataaaatga atctgcgaat
    14581 atttttgttg gtattactca ataatccacc actcacccca ttacttcttc aaatcctgca
    14641 aggtcttgaa aatacgcttt gccctccatt catcaatggt aaatacatac ggtgtttttg
    14701 aacaggtaca tatatagcga ttattttcat attctttatg gactgtaagg cttatctctt
    14761 cggcagcttt ttcattgttt tcattttgta ctgcaacggt aagcgacgca attttaccct
    14821 tcagcattgc agtatcaacc tgcgggaatg aatacgcctt taatggatca aaggtataca
    14881 gtatctcttt tactttatta gtatccagtt gcaatgnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnncaat atcaagctgc actatatcct tgttgcgcaa atcatccaca
    15061 gtcttgttaa aataagtatc aagcgtatct gctgctaaat ataccgcttt attcccttca
    15121 aaccgtacat aggtatgcct gtaggtactt ccttttttac ctacataaaa atcacgaacc
    15181 acagtatcac cacattttgc aactacatgt attgcatgtt cggggtctaa ctcaaatcgt
    15241 tcatacagcg gcttttcact gacaaaatca accacctcaa tatctttcag cttgttttca
    15301 atattagcaa taacattctt atcagcttca aacccattaa ccttccagct atcgccatct
    15361 ttgattattg ttatactgtt cccatccttt gcaataacta tctggctaat tgcgcatgac
    15421 acttcaggaa gctgcaaaag ctcacgagaa ctgaacttat ttaatagcag atagcctccc
    15481 agaacaacta tcaccacaat acttataaca atattttttt taacctgcat tgtactacct
    15541 ctgttacact cattatctca tcattgtata ctgtgattca atgtgtttcc ttcgcaattt
    15601 tcttcgccac atatacactg cgccagcaat aagcactaaa attggcaacc cggcaatatt
    15661 gaaacctttt atgatacttt ttgcagtatc tgatgtttct ttaattggat taaatgatag
    15721 tcccttgctt cgcatctgcg gtatatcata gtttccctgc atgtaatcta ccacattatg
    15781 cataaaaaca gcgttaggcg atttaccttc gtcatcgatg atattccttc cagtaatctc
    15841 ggatgttcca gccacaaaaa gtttaacagg tttaaccgtt tcctttatca cttcctgttg
    15901 tatataatct tggctttttg cgttctttcc ctttgcatct atggttggca ttttatcagt
    15961 aaaataactt ttgaggttac cctgaaccag tattgccaga ttgtacgacg caaggttatt
    16021 atccgcagga ggattgacac cccatggcat aaagtttatt gcaccctgtt gcagccagct
    16081 ataaggcgat gatttaacaa tatatttaac cgatgctttg tttgctttta acaagtcctc
    16141 gtcggcttcc aacgatgaag cttttaaaaa tacaatcttc tttaaatatt tagtaatctc
    16201 acagtcatca gataatcctt catcatctat cattggtgca aagtatatgg taagctcgcc
    16261 caaaccccgc tgttgagcaa taaagcactg tttatccatg actatatctt ttcttatttt
    16321 aattccataa tgtgccagca tcccctctaa accagtaata actggcaatg cctgcggagg
    16381 tctttgaaac atctgcatcc cttcagcctg ttgttcaaca tatgagtcaa gcaacagtat
    16441 tgcattgccg ccacgcatca aaaattgatc aatcttgaaa agttcatatt cgctgtacat
    16501 ggaacgaggg ccattgatta ttatggtgat aacatcatca ggtatttcat cctttgtaat
    16561 gtcaacagta acaaggtcat acatatccga cagcatcttc ctgaactgtg cagcgccgtt
    16621 ttgttcatca ttaatatcct tttcaccatg tccggtaata tagcaaactt ttggattgac
    16681 actgataagg ttatcgatag cagcgtttaa catatcctca agacgtgata aatcgccaat
    16741 ggcatattgt ccaaagattg aacgggtaag tatctgtacc gtttcaaact tattgttatg
    16801 ctcaacaact atcccaacca taccctgtcc tggaggtatg gctctacctt ccatggtggt
    16861 aaattgcggc cattttaacg ttggcagccc atacatctgt gctatggtca gcgcctgtgg
    16921 attattgacc ggatcaacaa agcggtattg gattttattg taattacgta tattacattt
    16981 ttgcacctga gcatacacct gctcatttaa attctgcatt ccagggatag gaagattgct
    17041 gctggcatat aacgtaacag taatgggatc tttaatcttt aaaagagcat ctattttccc
    17101 attcattttt tttatcaggg ttgttatgcg gtactccagc ccttcaggct cggtgattga
    17161 atctatcttt tctattatat cggcatggat gatagcaagc cccatataag catttctaaa
    17221 ttttacctga tcattgcgaa tctcacgtat ctgaacagga tatatcccaa agtcacccgc
    17281 agcatctttc tgtttttcaa catcaataaa ttcataatgg aaatacctgt tgcctgcatt
    17341 atcatactca accatcaaat cctgcaaata acgataaaca gtattatacg gcgccggcag
    17401 gtctttgtta aagaaaatct ttactgtcag aggttcttca agtgttgaaa ccactgcttt
    17461 gctgatgggt gatagtgaat aaacgctatt ttttgtgagg tctaaccgta catagagggt
    17521 taaaccaaca aggttaatca aaatgatgat aattccatat aaaataaaat gtgagtagct
    17581 tttttgtttt tgcggtttat caggtgcttt ccctattttt tgcattatgc gcctgagtgc
    17641 tgcatcaaac ctatcaacga ttttgttact gtattttttg atatttccca tgttcatagc
    17701 ctatcttctc acctccagct ggtatatgct tgccatagaa cttatgatga taaccgaagc
    17761 aaaatagata atattgcgcg tatcaataat accccgtgca atgttttgaa aatgataatc
    17821 tgcgctgata tattcaaata aagaaaccaa tttgggtgac acaaaatata aaaattttgt
    17881 tatgagcgat agtactatgc aaatagcaaa ccctatgata aacgctacta tctgatttct
    17941 ggttaacgat gaagcaaaca cgcctattga cgcatatgct gctgcaagga tgatagcacc
    18001 aataaatcct ccaattatag ggcctatatc aggtgaaccc acaaaagcaa ccgatactgc
    18061 gtaaaacaga gtgggtaata ccataatacc ggcaaaaatc gtagctgcta aaattttacc
    18121 ggtaacaatc tgcacagtgg aaagtggtaa tgtcataagc atttcaaatg aaccggtatt
    18181 ccgctcttcg gcaaaaaggc gcatggtaat tgcagggata acaaaggcaa ataaaagcgg
    18241 caatagttca aaatatgccc gcagttcagc ctgtccatat aaaaaaaatg tggaaaaaaa
    18301 caaaaagccg gcaatcacca gaaaaactga aatgacaata tagccaatag gtgttgagaa
    18361 aaaaccagcc aattcttttt ttgctactaa ctgtgcgccc ttcatactat ccttaaactg
    18421 ttgcatgcca atccctcctt aagctcctac cgttaattca cggaaaacat gttccagact
    18481 tctatattca cgattcattt cgtataatac ccagccatta tcttttatgc agttaaaaat
    18541 tgcagggcgt gcatctatat ccgaaccagt tatcacctgt acatggcaca gcccctcctc
    18601 atcgtgtata tggttcacat tattaatacc aggtatcgca cacaatgctg tctttaactg
    18661 gtcaaatgaa gcgccacctg ccagaatatt tattcggata tcctttcctt tggcgctctt
    18721 cagctcgctt gttaaatcat cagcaactat caccccctta tcaatgataa taactctgct
    18781 acacgttgcc tcaacctcct gcaagatgtg ggttgataga ataaccgtct tttccctgcc
    18841 caactccttg ataaggtcac ggatttcaat tatctggttg ggatcaagcc cattagttgg
    18901 ttcatcaagt atcaaaattt cagggtcatg caaaatagcc tgcgccagcc ctacccgctg
    18961 tttataccct ttggaaagct cattgatatt tttgtgcatt acctcggtta ttccgcattg
    19021 tatagcaaca tccgttatcc tttcgacatc attgatctct ctgatatcag caataaattg
    19081 taaaaagtca tataccatca tatcaggata aagcggagcc gattcgggaa gatagc
//
LOCUS       DAOSOX010000025        19015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2906_length_19015_cov_9.099051, whole genome shotgun sequence.
ACCESSION   DAOSOX010000025 DAOSOX010000000
VERSION     DAOSOX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19015
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2906_length_19015_cov_9.099051"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /gene="ade"
                     /locus_tag="PLX22_03330"
     CDS             complement(<1..1553)
                     /gene="ade"
                     /locus_tag="PLX22_03330"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOT18969.1"
                     /translation="MNFIPLETITMELADVAMGRRKADMVIKNGTLVNVNTKELQEHI
                     DVALYKGRIALVGDASHCIGETTQIIDATGLYIAPGFMDGHIHVESSMLTVSQYSTAV
                     VPHGTTAIFMDPHEIANVLGLKGVKLMLDEARDVPLRVYATVPSCVPAAQGLEDAGAE
                     IGPEEIAEAMKWDAIAGLGELMNFPGVFNSVPDVHKEIAVTLKAGKPVTGHFALPDTG
                     KMLNAYIASGVRSCHESVRHEDALAKMRLGMYAKIREGSAWHDLKEVIKAITHNTVQS
                     RFAILVSDDTHPDTLIKLGHLDHIVKRAIEEGVDPVTAIEMVTINTAECFLMSKDFGS
                     VSPSKVADIVLLSDLYSVTVKAVIIGGRLVARDGTMLSSAKKVTYPDWSKNTVNVGKT
                     LTPQDFMLTVNKSEVKVRVIQIEEAKVTTKQVIETLKTIDGNVAPNKEKDIAKAALFE
                     RHKATGTKGLGFVKGFGLKKGAVASTVAHDSHNLLIVGTDEEDMAFAGNELIKAGGGM
                     IAVADGKVLA"
     gene            complement(1566..1937)
                     /locus_tag="PLX22_03335"
                     /pseudo
     CDS             complement(1566..1937)
                     /locus_tag="PLX22_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
     gene            complement(2017..3471)
                     /locus_tag="PLX22_03340"
     CDS             complement(2017..3471)
                     /locus_tag="PLX22_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968717.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOT18970.1"
                     /translation="MWSISLLTIFLALMVIIGIWGMRKTSTLGDFFLGGRTIGPWVSA
                     LAYGTSYFSAVLFIGFAGKLGWVYGLNAVWIGVGNALFGSLLAWLVLGRRTRIMTQNL
                     DVMTMPEFLMERFDGKYIKMFAAVIIFIFLIPYSASVFKGLGHLFEVNFNISYDLALI
                     IMIGITGIYLVLGGYFAITLTDFIQGIIMVAGALTMVLILSSKNGGIVQSIALVAEKY
                     PLHVPAGKQPGWILLSAFIFMTSFGTWGLPQMVQKFYAIKSQDLIKKAAIVTFCFATI
                     IGVCAYFTGSLSHLFYDAVPVVGNKPAFDLLIPDLLTKHLPEPLMAVILLLILSASMS
                     TLSSLVLVSSSAIAIDLYKGHVNPQITKENSVAMMRFLSALFVILSYFTARYEFGIII
                     TLMSLSWGAVAGAFMAPYLYGLFWKRTTLAGVYAGMITGLVVSIGLFFYLGAEKSAVA
                     SSLGMIIPFFIVPLVSVLTKKPDGALIQKAFEKI"
     gene            complement(4309..6015)
                     /locus_tag="PLX22_03345"
     CDS             complement(4309..6015)
                     /locus_tag="PLX22_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT18971.1"
                     /translation="MLLFNPKKYERHHADDRSKQIMLKTIEFFEQKGLKKIKEDDQAA
                     VWYDDFLEFIKKEQTFATLLTPSGYGDKDSRWDMWRIEEYNEILGFYGLCYWYAWQVS
                     ILGLGPIWMGQNEEVKHKAAKLLKDGGIFGFGLSEKEHGADIYGTDMMLYPKGDGTYL
                     ARGDKYYIGNGNAAALLSVFGKMADTGEYVFFVVDTKHPNYECVKKISTSGVRQAYVA
                     EFALHDYPITEADILSRGNHAWDSSLNTVNVGKYELGWASIGICEHAFYEAIDHAANR
                     RLYNMYVTDFPHVKKIFTEAFARLAAMKLFALRACDYFRTANPDDRRYLLYNPIQKMK
                     VTMEGEKVIGLLHEVIAAKGFEQDTYFEMAIRDIGMLPKLEGTTHVNMALIVKFLQNF
                     MFKPKEYPEVPRRDDPANDSFLFNQGPAKGLGSIQFNDYRKAYEGIKSNNLEIFLEQI
                     EAFRNFLIKGTPDEKQTKNIDYMLSVGELFTLIPYTQLICENKKIYNIEDEIIDEIFK
                     FIVSDFSAYATRLYTGQKNSDKQNELIVKMIKKPVLDDAIFNKVWNEQVYALKGQYTM
                     NE"
     gene            6287..6607
                     /locus_tag="PLX22_03350"
                     /pseudo
     CDS             6287..6607
                     /locus_tag="PLX22_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6660..7646
                     /locus_tag="PLX22_03355"
     CDS             6660..7646
                     /locus_tag="PLX22_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT18972.1"
                     /translation="MVYTLGDVPYWGLAGVITVRLDDRVETIALARTFQAVALAITTV
                     LSPLMAHWLSFSDKTTAIGWTRTAIIVSIIGMGLFTLAFFNTTEKVPLQHNKQTIRQM
                     VNAIITNKPLLLILLGSALNFGRNIVQVGGAVVASIVFGDEKIFSLLGGVLIAAIIIS
                     NLLTPLVLRVMSKKMLMIISSLLSALTYTAMYFIGYSNFWLFVFMLFVSGFFTGFFIV
                     IQTAMIADSVDYYEYKTGSRNEGVSFAGLTFISKLMGALATMVFGMVVVAVGYSTGVV
                     ITPYIKDGIFFSITILPAISCAIGIIPFLFYPLGDSTMSEILSQLQQQRGSA"
     gene            complement(7683..>8486)
                     /locus_tag="PLX22_03360"
     CDS             complement(7683..>8486)
                     /locus_tag="PLX22_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOT18973.1"
                     /translation="LTLVHCETFVDGPMAVRRNYYNHKKTTLRQDVTVYAGKPYIEFD
                     TECDYHETFKMLRADFMPLVFDDTVLCDIQFGHYGRSTKDGTSLERAQYEICAHKWVD
                     VSNSNYGVALINDCKYGHRVKEGVISLNLLRSPVYPDPSADRGKHVFKYALYPHTGDF
                     KSCDTLCYGYIFNYAPRLADGTVTIPQIVCTNNNRIIIETVKKAELQDAIVIRAYECY
                     GNDTIASFDVSFNVKQAYEADMLENVLNKTNLQSVTFTPFEIKTFVLQL"
     assembly_gap    8487..8586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8587..10469)
                     /locus_tag="PLX22_03365"
     CDS             complement(<8587..10469)
                     /locus_tag="PLX22_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOT18974.1"
                     /translation="MKGEWGIYKHPVLLYHLHKIKKHIYTRVADVTVSITTDSEPIPY
                     DERLNRNYRPLRKGDVWGKAYDCAWLHVKGVIPAGLASSHIVVIVTIDGEGVCYNNGM
                     EVCAINSRCTFMDYLQPTWDRRVIAIDDATSCGLLDVWIDISFNGKMVQPFGKAKFHD
                     AYIALCRDDIKDMYYEYLTYCYACLACDDTAMRHRLLSYGDAVYKALQGYLPPDVAKA
                     KAILNEAYTIVGGTSDYGVTAVGQGHLDLAWLWPVRESKRKAVRTFVNALRTIDQYPE
                     FVFGASQPQQFEWIKLNDPGLYMKIKQAVATGNIELQGGMWVECDTNLPCGESLIRQI
                     YYGKKFFYDEFGMDIVTCWLPDAFGFNANLPQILKKCGIHYFATIKLAWNEYNKFPHT
                     TFNWVGIDGSSVLAHMPPEGDYTSGATPLCVKQALDNFIEKDRLGTALLVYGNGDGGG
                     GPTQTHVELIKRQQTVQWLPRVEFGRAVDFFKRLEHYKDVLPVYKGELYLEKHQGTYT
                     TQAQNKYYNRKLELLLHECEYLAACAYLKGYSYPHALFDRIWKEVLLYQFHDILPGSS
                     VKRVYDESTMRYEAMTVELEDAIKNSIRYLSDDSHGKSCINAAPFARTEFFKVDSHWY
                     SAE"
     gene            complement(10631..12172)
                     /locus_tag="PLX22_03370"
     CDS             complement(10631..12172)
                     /locus_tag="PLX22_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT18975.1"
                     /translation="MKLSDYFLARYTENSFMIQEKARALLYFIMAFIVLIPIIIISVN
                     IVQPFSSDNVVFVILLLASVIGLILLKKGQYNASANLIITFASILIVVFMFYKAFESK
                     DADFISFTYYMPVIIILTALFCRIRWVIGISLFFIVSTIVCTVIMSTILTDIYSRVLK
                     DLAVDYLFSITLSFVLCLLIVRINTRNLQQLKKESEENVKQRDVIKNLLVSVQGLSQQ
                     LTALAEQSSESSAVFSNGAQSQAASAEEITSTVEEMTAGIESISDSVTKQFDMITRLI
                     VRINELSMFIEGMGTRVNVSAGKAEDVSKQGSLVEGSLNTMNESMGSIMKSSQDMNNI
                     IEIINDIADQINLLSLNAAIEAARAGDAGRGFAVVADEISKLADRTTTSIKEIASLIT
                     INVKEIQSGFANIEQMTANIKKMVTSVAMISDEMKQLFESMQQQLKDNEAVRSDAQTL
                     KNFSDSILSAIEEQKTAAMEIAKSVATVNETAQGNAAGSITLAENAHELLQLAKGLQD
                     EIAHV"
     gene            12723..13343
                     /locus_tag="PLX22_03375"
     CDS             12723..13343
                     /locus_tag="PLX22_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT18976.1"
                     /translation="MVDAIHWLGHDTFKIVKDVVIYTDPFKIKNKDNADIILITHEHF
                     DHCSPDDIKKLQTNDTVIIAPKDCIAKLSGNVKAITPGKSITVKGITIEAIPAYNTNK
                     QFHPRSNQWAGYIITIDTMRIYMAGDTDYIPEMKSLKNISIALLPVSGVYVMTADEAA
                     QAALTIQPAVAIPMHYGSIVGTIADAKKFESLLKGKVQVVIKKQEN"
     gene            complement(13497..13979)
                     /locus_tag="PLX22_03380"
     CDS             complement(13497..13979)
                     /locus_tag="PLX22_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18977.1"
                     /translation="MYFETTTCVSHNHLHCIARLADQYDMSIRSFIVYMIIYAAKKEK
                     APSKAFKSISYRDRKKDNPWKRLHLYLQYGEYEYLLDVKKVWKMSVAKVIEFCMENVL
                     DEFLAFLNEIDQEVKRGNTDNYLRYEINRSYMFDFCIDEGVHCCRFYWGLPMKYARAK
                     "
     gene            14160..16160
                     /locus_tag="PLX22_03385"
     CDS             14160..16160
                     /locus_tag="PLX22_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOT18978.1"
                     /translation="MQPLKKFIAIIILFISSSALIQCSTSTKITIHYSPAHYELSNSF
                     LAGASKADITPPPGKPLAGYSSNANFAKGFRTRLYARVIYLKSPLHNAIALVQCDLLS
                     GSSLVQQRVAELVASTTDVAYNGIVIAGNHTHSGPGNYFESNFYNRFASNASGFDEDY
                     FNFLCNQIAQAIIEAYNNRKPGMIAYGEKTLYGFTRNRSITAYTANANANSSNPLKAV
                     NPILSMIRIDTVENRSIHPIAALSIFSIHGTAVPDSNDLYSADVFAYIERELELEMQR
                     NGATTFIHAVVNGTHADNAPNIMKGKQGFQEARRIGIQIGHHAIELFNELSAKLSSHV
                     DSKSVIQFIDVYAHPTVDGATLCIPPKVGNTLLAGASDGGPTPIISSTPFFMEGSRRF
                     IFTCGCQKNKRIAAGPLQPLILPGDDFPHILTFQSIAINQLLLLPVPFEVTCESGKRI
                     ANAALTASPHTGIVTPIVISCANGYTGYCTTPEEYAKQRYEGGHTLYGPSTQPYLAKQ
                     YAQLSVTIAQNSMSDSSWTNCYVLKLASFYKKYPMPTTIRREALSKPVYVCNPGIDET
                     YVEFTWSDLPPDYMEFHQSLVSIEYSTDGIQFHPFIKNGITVDDTGYDIAVLFTGTIT
                     KEGNALYTTRWYNPELAENVYFRFAIQPRQKLPFVYSEPFSW"
     gene            16518..17267
                     /locus_tag="PLX22_03390"
     CDS             16518..17267
                     /locus_tag="PLX22_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOT18979.1"
                     /translation="MTLKNKILIILSLCLLLCIGAYFIISRTFANFEKQLFEKCRIEA
                     LVGGRVMSEIIDMMIDTRLLTKQQVFDRNYVPIQGSNPQKFRTQYDAVFDKYIQKIED
                     EFLKDKDLEFAVLVDINGYLPTHNSKYAKPETSDPDHNLKYSRSKRIFNDRVGLKASR
                     FIGPGTLKQLYTRDTGEVMWDISSPVFVKGEHFGAFRLGVSLKHINELKNQMIIIVGM
                     TILVILSITMLMLFLILPRKLYDTDLDIPQY"
     gene            complement(17264..18061)
                     /gene="folE2"
                     /locus_tag="PLX22_03395"
     CDS             complement(17264..18061)
                     /gene="folE2"
                     /locus_tag="PLX22_03395"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOT18980.1"
                     /translation="MPRKSLKDIQSQPDDRNLPINKVGVKDITYPVVVMDRADGLQHT
                     VARINMYVDLPEHFRGTHMSRFVEVLNRYQGGISTKNVRNILDDLKGELNAKTAHVTI
                     EFPYFIVKAAPVSGEKSMMDYLCILQGDSSVDYYTLQVKVPLLSVCPCSKEISRYGAH
                     NQRNVVCVTVRAKEILWIEEFIEIVEHCGSSEIYALLKREDEKYITERSYENPVFVED
                     IVRNVAIKLLAHPKVLWFQVETESMESIHNHSAYAQIEIDKQKKGQR"
     gene            complement(18071..>19015)
                     /gene="recA"
                     /locus_tag="PLX22_03400"
     CDS             complement(18071..>19015)
                     /gene="recA"
                     /locus_tag="PLX22_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014128112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT18981.1"
                     /translation="SIMRLGDEHASMKVPSISTGSLELDIALGIGGIPRGRITEIYGP
                     ESSGKTTLTLHVIAEAQKLGGIAAFVDAEHALDPTYAKKLGVNTDELLVSQPDTGEEA
                     LEITESLVRSGAIDIVVIDSVAALVPRAEIEGEMGDAHMGLQARLMSQALRKLTAVIA
                     KSKTSVVFINQIRHKIGVMFGSPETTTGGNALKFYASVRLDIRRVDTIKVGEDAVGNR
                     VRVKVVKNKVSPPFRQAEFDIMYDSGISRTAGILDLGVKYNIIQKAGTWYSYKDERIG
                     QGRENAKSYLDQHPDSAKEIEEKIKQQAGLTAQEEVKA"
BASE COUNT         5670 a         4243 c         3263 g         5739 t
ORIGIN      
        1 gccagcactt tgccatcagc aacagctatc atcccaccac cagctttgat aagctcattc
       61 ccggcaaaag ccatatcctc ttcatcggta ccaacgataa gcaaattatg cgaatcatgt
      121 gcaacagttg atgcaacagc gccttttttt aagccaaaac cttttacaaa acccagccct
      181 tttgttccgg tggctttatg acgctcaaac agcgcagcct ttgcaatatc cttttcttta
      241 ttgggtgcaa cattgccatc aatagtcttc aatgtttcaa tgacctgctt tgttgtaact
      301 tttgcttctt ctatctggat tacacgaact ttaacctcgg atttattgac tgttagcata
      361 aaatcctgag gggttaaggt tttgccaaca ttaacggtgt tttttgacca gtcaggatag
      421 gttacctttt ttgccgatga aagcattgtt ccatccctgg caaccagcct gccgccaata
      481 ataactgctt tcacagtaac actataaaga tccgaaagaa gaacaatatc agcaaccttt
      541 gatggcgata cacttccaaa atccttgctc atcaaaaagc attcggcagt attaatggtt
      601 accatctcta tagcagttac agggtcaaca ccttcttcaa ttgctctttt aacaatatga
      661 tcaagatgtc ccagttttat caacgtatca ggatgggtat cgtcactcac aaggatagca
      721 aaacggcttt gcactgtatt atgggtaata gcttttatga cctcttttaa atcgtgccat
      781 gctgaacctt cacgtatctt tgcatacatt cccagacgca tctttgccaa tgcatcctca
      841 tgcctcaccg attcatgaca tgaacgcacg ccagaagcta tgtatgcatt gagcattttt
      901 cccgtatcgg gcaaagcaaa gtgacctgtt accggtttgc cagcttttag cgttactgct
      961 atctctttgt gtacatccgg gacgctgttg aatacaccgg gaaaattcat aagctcaccc
     1021 aaccctgcaa tggcatccca cttcattgct tctgctatct cttcagggcc aatttcagca
     1081 cctgcatcct ccagcccttg tgcagccggc acgcatgaag gcactgtagc ataaaccctg
     1141 agcggcacat cccgtgcttc atcaagcatc agtttcacac ctttaagccc cagcacatta
     1201 gctatctcat gcgggtccat aaagattgcc gttgttccat gtggtaccac tgcagtgctg
     1261 tactgtgata ccgtcagcat gctgctttca acatgaatat gcccatccat aaatccaggt
     1321 gcaatatata aaccggtagc atcaattatt tgcgttgttt cgccaataca gtggctggca
     1381 tctcctacca atgctattct tcctttatat agggcgacat cgatatgttc ctgcaactct
     1441 tttgtattga cattcaccaa tgttccattt ttaattacca tgtcagcctt acggcgtccc
     1501 atggcaacat cggcaagctc catggtaatc gtttcaagtg gtataaagtt cataaagtta
     1561 tccccctatt gaatttgttg cactatagta ttgcaatcaa agagcatacc atcatctttt
     1621 tttgcaatca ttttcatgtc gtttgtaaat ccggttttac taaaaagcat atagcagtta
     1681 ttttgatctt tgcaatagac aatgtccctt gatattttta ctaaaatcag gatatatagt
     1741 tttttattgc tccatgtgca ctcaccaaac cacaccgtat cagtatcatc atccagagca
     1801 acaatgtcaa tctcattatc ttttgaccac cacctgccta tacagttaca ttgcagtaat
     1861 tgtttttgca tcagagcatg gcatagttct aaacaggcgt cttcaaaggt taaagaaaca
     1921 tgatgctcaa cattatcctt aaatttttta acacaatatc aggttgttca cgttcaatcc
     1981 atcgcctatt gggaaatata aaatgaaaac aagttatcaa attttttcaa acgctttctg
     2041 aattaaagcc ccatcaggct ttttcgttaa tacgctcacc agtggtacaa taaaaaatgg
     2101 gatgatcatt cctaaactgg acgcaacggc agacttttca gcacccagat aaaaaaacaa
     2161 accaatagac accacaagcc ctgtaatcat accagcatat accccggcaa gggttgttcg
     2221 tttccagaac agtccataca aatacggtgc catgaatgcg cctgcaacgg ctccccatga
     2281 aagcgacatc agggttataa ttattccaaa ttcatatcgt gcagtaaaat atgataatat
     2341 gacaaacaag gcactcaaaa agcgcatcat tgctacagaa ttctcttttg taatttgtgg
     2401 attaacatgc cctttgtaaa ggtcaattgc aatagcagat gaagatacta atacaagtga
     2461 tgaaagagta gacatggatg cagaaagaat taacagtaat atcacagcca ttaaaggctc
     2521 cggcagatgt tttgtcagta aatcagggat gagcaggtca aatgccggtt tattaccaac
     2581 aacaggaact gcatcataaa acaggtgtga tagcgaaccg gtgaaatacg cgcaaacacc
     2641 aataattgtt gcaaaacaaa atgttacaat agcggctttc tttataagat cctgactttt
     2701 aatagcataa aatttttgca ccatctgcgg caagccccat gtaccaaatg atgtcatgaa
     2761 gataaatgca gaaaggagga tccatcctgg ttgttttcca gcaggcacat gtaatggata
     2821 tttttcagca accagtgcaa tactttgaac tatcccacca tttttagatg atagtatcag
     2881 taccatggtg agagctcctg caaccataat aataccttga ataaaatcgg tcaatgtaat
     2941 agcaaaatac ccacccagca caaggtaaat accggtgata ccaatcatta tgatgagtgc
     3001 taaatcatac gaaatattga aattaacttc aaaaagatgt cccagacctt taaaaacaga
     3061 tgcagagtac ggaattaaaa atataaatat aatgacagca gcaaacatct taatatattt
     3121 gccatcaaag cgttccatta aaaactcagg catggtcatg acatctaaat tttgagtcat
     3181 aattctcgta cgtcttccca gcacaagcca tgctaacagt gaaccaaaca gcgcattacc
     3241 aacgccaatc cagacagcat taagcccata tacccagccc aatttgccgg caaagcctat
     3301 aaaaagcact gctgaaaaat acgaggtgcc atacgccaac gctgaaaccc atgggccaat
     3361 agttcggcca cctaaaaaga agtcccccag tgttgatgtt ttacgcatac cccagatacc
     3421 aataataacc attaaagcca gaaagattgt taacaatgaa attgaccaca tagctaccga
     3481 tctccttttt tatttttaat cgatcatcat gcctggcaat tacattgcca atggaatgtt
     3541 gtactaaaat tactttgaat ttatatttct atcaagaaag caggttgtca ataataaaat
     3601 aattttgatg aaggatctat attgcaatga ggaatagtga ctctataaac aaatcaatct
     3661 ccagagggag tataataact agagagaata aatataaata caagtgaaag cattatcctt
     3721 ttatcaattg atttctgtat attatcactc atatcaagaa tataattact acgaaataga
     3781 gtaaactctc tggtcaggct cgctaaaaca tccctgtctt tgactattaa aaacctcctg
     3841 tttggaaacg aatggagtat agaatcatct ttttttctca tcatacacat acatttttgg
     3901 agagaaatat cctcttactg gaataaaaaa tgtatctcta tcaagaactt catttatctg
     3961 atccatattt cttcttccaa taaccagcaa tgcttactat ttaatctgca tgcatttttt
     4021 taagtaattt tttgcactgt aatgattatt tttttcgata acaatatttt tttatatttg
     4081 tgatatgttt caaatttgaa gttacaacca atacccggca ccagaacatt gccatataaa
     4141 atagctatag tattatttca gcaaggaaac ttaacccttc actattttga agatttttat
     4201 gattttttta ttacagatga ttgtataata atgggtgagg gtttgatatt ccataacaaa
     4261 atttaaaagg acaccatgtt ctggtgtcct tatattcata gtaaactttt actcattcat
     4321 ggtatattgc cctttcaatg catacacctg ttcattccat accttattaa aaattgcatc
     4381 atccagaaca ggttttttga tcatctttac aataagctca ttttgcttat cgctgttttt
     4441 ctgccctgtg taaagccgtg ttgcatatgc agagaaatcg ctgactatga atttgaaaat
     4501 ctcatcaatg atctcatctt caatattgta aatcttctta ttttcgcaga taagctgtgt
     4561 atagggaatc agtgtaaaca gctcgcccac cgatagcata taatcaatat tctttgtttg
     4621 cttttcatcc ggagtcccct taataaggaa gtttctaaat gcttctatct gttctaagaa
     4681 aatctccaga ttgttgctct tgatgccttc atatgctttt ctgtaatcat taaattgtat
     4741 cgatcccaat ccctttgccg gtccctgatt aaacaaaaag ctatcattgg caggatcatc
     4801 ccggcgaggc acttccggat attcttttgg cttaaacata aagttttgta aaaattttac
     4861 aatgagtgcc atattaacat gcgttgtgcc ttccagtttg ggcagcatcc cgatatctcg
     4921 aattgccatc tcaaaatagg tatcctgctc aaagcctttt gcagctatga cctcgtgcag
     4981 caacccaatt accttttccc cctccatggt taccttcatc ttctgaattg gattatacaa
     5041 aaggtatctg cgatcatcag gattggcggt acggaaataa tcacaagctc gcaaagcaaa
     5101 cagcttcatc gccgccaacc gtgcaaaagc ctcagtaaat attttcttaa catgcgggaa
     5161 atcggttaca tacatattat aaagtcttct gtttgctgca tggtcgatag cttcataaaa
     5221 tgcatgctca catatcccaa ttgaagccca ccctaactca tacttgccga catttactgt
     5281 gttaagcgat gaatcccatg catggttccc acgtgataag atatccgctt cggtaatagg
     5341 ataatcatgg agggcaaact cagcaacgta cgcctggcgt actccagagg tacttatctt
     5401 tttaacacat tcataattag gatgttttgt gtccaccaca aagaatacat attcaccggt
     5461 atcagccatt ttgccaaata ccgaaagaag cgcagcagca ttcccattcc caatataata
     5521 tttatcacca cgcgctaaat aggtaccatc acctttggga tacaacatca tatcggtgcc
     5581 atagatatca gcaccatgct ctttttcaga aagcccaaac ccaaatatgc caccatcttt
     5641 caataatttt gctgccttgt gttttacctc ttcattctgc cccatccaga ttgggcccaa
     5701 acccaatata gatacctgcc atgcatacca atagcatagt ccataaaaac ctaaaatttc
     5761 attatactcc tcaatgcgcc acatatccca ccgtgaatcc ttatcaccat accctgaagg
     5821 cgtaaggagt gttgcaaagg tctgttcttt ttttatgaac tctaagaaat catcatacca
     5881 tacggctgct tgatcgtctt ctttgatctt cttcaatcct ttttgttcaa agaattcaat
     5941 agttttcagc ataatctgct ttgatctatc atcagcatga tgacgttcat acttttttgg
     6001 attaaatagt aacattgtta ccccctctcc acttgtaata tataatacaa attagtatat
     6061 actatttatg attcttattg tttaatatag cactacatca aaaaatttaa gtcaacaata
     6121 aaaatatatt attttttcaa cacaaatatt gtactattgc cagccatact taccaaaatg
     6181 tcaggataca gttctttaca taaaaatttc atgcatatca aatatattga caaaacgatt
     6241 gtacaccatt tactattgaa aactcttatc ataaaaggag caatgtatgg attatagtac
     6301 caaactatca aataaagaaa aatattcatt tgctatagcc ggttttggac aaaatatggt
     6361 aataacattt acaacaacat tcatgttagt gtatctctat aattttgtcg gcttcagcct
     6421 atcggggatt gctaccctta ctgctataat aggagctgca aaaatatggg atgcactcaa
     6481 tgatctcata atgggcatgt ttgtagatta tacccatacc cgatggggta aattacgacc
     6541 atatatactc ctatcagctt ttcctattgc tctgcttacc atattgctat ttgctatccc
     6601 acaaactact gaaacatata agcttatctt tttttgctat tgtttacatc ttatgggata
     6661 tggtctatac cctgggcgat gtcccctact ggggactggc aggagtcata accgtaagac
     6721 tggacgacag agtagaaact attgcattag caaggacatt ccaggcagta gcactggcca
     6781 ttacaactgt cctcagtccc ttgatggctc actggttaag cttttcagat aaaaccactg
     6841 caatagggtg gacacgcaca gcaattatag tatcaataat aggcatggga ctttttaccc
     6901 tggcattttt taataccacc gaaaaggttc cccttcaaca caataaacaa acaatacggc
     6961 agatggtcaa tgccattatt accaacaaac cacttttgct tatcctttta ggcagcgcat
     7021 taaactttgg gcgaaatatt gtacaggtgg gaggtgctgt tgttgcatcg atagtttttg
     7081 gtgacgaaaa aatattttct ctgttaggtg gtgtgcttat tgcagctatt attatatcaa
     7141 acttacttac tcctctggtg ctaagagtta tgtctaaaaa gatgcttatg attatttcaa
     7201 gcctactgtc agcattaaca tatacagcga tgtattttat tggctatagc aacttctggc
     7261 tgtttgtttt catgcttttt gtttcaggat tttttacagg atttttcatt gtgatacaaa
     7321 cagcaatgat tgctgattcg gttgattatt atgaatataa aaccggatcg cgcaatgaag
     7381 gggtaagttt tgccgggtta acatttattt caaagctcat gggggcactg gcaaccatgg
     7441 tttttgggat ggttgttgtt gcagtagggt attcaaccgg tgttgtcatt acaccttata
     7501 tcaaagatgg cattttcttt tccataacga tactgcctgc catcagttgt gctattggca
     7561 ttatcccatt tctcttctat cctcttggtg atagcacaat gagtgaaatt ctatcacaat
     7621 tgcaacaaca acgtggcagt gcataatcag gtgatgataa aaaatttata ataatcgttg
     7681 cattatagct gtaatacaaa cgtttttatc tcaaagggag taaatgtaac agattgtaaa
     7741 tttgttttgt tcagtacgtt ttccagcata tctgcttcat acgcctgctt aacattgaag
     7801 ctcacatcaa aagaagcaat ggtatcgttg ccataacatt catacgcacg tataacgatt
     7861 gcatcttgca gttctgcttt tttaactgtt tcaataatga ttctgttgtt attggtacaa
     7921 actatctgcg gtatagtaac cgtaccatca gcaagccttg gggcataatt aaatatatat
     7981 ccatagcata gagtgtcaca gcttttaaaa tcacctgtgt gcggatacag tgcatactta
     8041 aagacatgct tccccctgtc tgctgacggg tcaggataaa caggtgagcg cagcagatta
     8101 aggctgataa caccttcttt aaccctgtgc ccatacttac aatcatttat taacgcaact
     8161 ccataattgc tgttacttac atcaacccat ttatgcgcac aaatttcata ctgtgcccgt
     8221 tcaagcgacg ttccatcttt tgtacttctg ccatagtgtc caaactgaat atcacacaaa
     8281 acagtatcat caaacaccag aggcataaaa tcagcacgca gcatcttgaa cgtttcatgg
     8341 taatcacatt cggtatcaaa ctctatataa ggttttccgg catacactgt tacatcctgc
     8401 cgcagggttg tttttttatg gttatagtaa tttctgcgca ccgccatggg accatcaaca
     8461 aatgtttcac aatgaaccag cgttagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnattt cagcactata ccagtgacta tcgaccttaa aaaactccgt gcgcgcaaat
     8641 ggtgctgcat taatacatga tttcccatgg ctatcatcag aaagataacg aatactgttc
     8701 ttgattgcat cctcaagctc aaccgtcata gcctcataac gcatggtgct ttcatcatat
     8761 acccgcttaa cgcttgaacc gggtaagata tcatggaact gatacaaaag aacctctttc
     8821 catattctgt caaatagcgc atggggatag ctgtaccctt taagataagc gcaggctgca
     8881 aggtattcac attcatggag cagcaattct aacttccgat tatagtattt gttttgagcc
     8941 tgcgtggtgt aggtgccctg atgtttttcc agatacagct cgcccttata aacaggaagc
     9001 acatccttat agtgttccag ccgtttaaaa aaatcgaccg ctcttccaaa ctccacccgt
     9061 ggcaaccatt gtactgtttg ctggcgcttg ataagctcaa cgtgtgtctg cgttgggcca
     9121 cctccaccgt cgccattacc gtaaacaagc aatgccgttc ccaacctgtc cttctcaatg
     9181 aaattatcca gagcttgctt tacacacagc ggtgtagcac cactggtgta atcgccttcc
     9241 gggggcatgt gcgccagcac actgcttcca tcaataccaa cccagttaaa cgttgtatgg
     9301 ggaaatttgt tatattcatt ccatgccagc ttaatagtgg caaaatagtg tatcccgcat
     9361 ttttttaaaa tctgcggcag atttgcatta aatccaaatg catccggcaa ccagcaggta
     9421 actatatcca ttccaaattc atcataaaaa aattttttgc catagtaaat ctggcgtatt
     9481 aacgattcac cgcaggggag gttggtatca cattcaaccc acatgcctcc ctgtaattca
     9541 atatttcctg ttgccaccgc ctgctttatt ttcatgtaaa ggccgggatc atttagtttt
     9601 atccattcaa actgttgtgg ttgacttgca ccaaaaacaa attccggata ttgatcaata
     9661 gtacgcaaag cgttaacaaa tgtacgcacc gctttgcgct ttgattcccg taccggccat
     9721 agccatgcta aatcaagatg cccctgccca accgcagtga caccataatc tgaagttcca
     9781 ccaacaatgg tatatgcttc attaagaatg gctttagcct ttgcaacatc cggtggcaga
     9841 taaccttgaa gcgctttata gaccgcatca ccgtatgata gcaaccgatg tcgcatggcg
     9901 gtgtcatcgc aggctaaaca agcataacaa taggtcagat attcatagta catatctttt
     9961 atatcatcac ggcagagggc aatatacgca tcatgaaatt ttgccttgcc aaagggctgg
    10021 accatcttcc cgttaaagct tatatcaatc caaacatcca gcaatccaca gctggtggca
    10081 tcatcaattg caataacccg cctgtcccat gtaggctgca gataatccat aaacgtgcat
    10141 ctgctattaa tggcacagac ttccattcca ttattgtaac acaccccctc accatcaata
    10201 gtaacaatca caacaatatg gctggacgca agccctgcag gtataacccc tttcacatgc
    10261 agccacgcgc aatcatacgc tttcccccag acatcacctt ttcttaatgg gcgatagttc
    10321 ctgtttaagc gttcatcata cgggattggt tcgctgtcgg tagtaataga gactgtaaca
    10381 tccgcaactc tggtatagat atgttttttt atcttatgta aatggtaaag caaaacagga
    10441 tgtttataga taccccattc acctttcatt gtattattat ataccactca gtataggttt
    10501 catggcgata attctcatca gaatatacac caacaaaaca atcattgcct catatggtaa
    10561 atcttcctga acatataacc aacaaaatca catgtgaata tatttatagc aaaaccgggc
    10621 attaatatgc ttatacatga gcaatctcat cctgtaagcc tttagcaagc tgcaataact
    10681 cgtgtgcatt ttcagctaag gttattgaac cagcagcatt accctgcgct gtttcattga
    10741 cggttgccac cgatttggca atctccatgg ctgcagtctt ttgttcctca attgccgata
    10801 aaatactatc tgaaaagttc ttcaatgtct gtgcatcact gcgtaccgct tcattatctt
    10861 taagctgttg ctgcatcgat tcaaaaagtt gtttcatttc atcagaaatc attgccaccg
    10921 atgttaccat cttcttgatg tttgctgtca tctgttcaat gttggcaaaa ccgctctgta
    10981 tctctttcac attaatagta ataagcgatg ctatctcctt tatacttgtt gttgtcctat
    11041 ctgccagctt cgatatttca tcagctacaa cagcaaaccc tctgccagca tcccctgcac
    11101 gggctgcttc aatggcagca ttgagcgaca gtaaatttat ttgatcggca atatcattga
    11161 ttatttcaat gatattattc atatcctggg agcttttcat gatactgccc atactctcat
    11221 tcatggtatt gagcgaccct tccaccaatg atccctgctt tgatacgtcc tctgctttgc
    11281 ctgctgaaac atttacccgg gtgcccatcc cttcaataaa catggaaagt tcattgatcc
    11341 tgactatcaa acgggtaatc atatcaaact gctttgttac tgaatcgctg atgctttcaa
    11401 taccggcagt catttcctct acggttgatg ttatctcttc tgcggaagct gcctgtgatt
    11461 gtgctccgtt tgaaaataca gcagaagatt cagaactttg ctccgcaagt gcagtcaact
    11521 gctgtgacaa accctgtacc gataccagta aattttttat aacatcacgt tgcttaacat
    11581 tttcttcaga ttcttttttt aattgttgca aattacgtgt gtttatcctt actattaaca
    11641 aacacagtac aaatgacaaa gtaatggaaa agagataatc aacagctaaa tcttttaaaa
    11701 cacgtgaata aatatctgta aggatagtac tcattattac agtgcataca atggtgctaa
    11761 cgataaaaaa taagcttata ccaataaccc atcgtatgcg acagaacaaa gctgtgagta
    11821 ttatgatcac tggcatataa taggtaaatg atataaaatc tgcatccttt gattcgaatg
    11881 ctttataaaa cataaacaca acaatcaaaa ttgatgcaaa agttataatc aaattggcac
    11941 ttgcattata ttgccccttt tttaaaagaa tcaatccaat aactgatgca agaagtaata
    12001 ttacaaaaac aacattatct gatgaaaaag gttgcacaat attaacagaa atgattatta
    12061 taggaataag cacaataaat gccataataa aatataaaag cgctctggct ttttcctgta
    12121 tcataaatga attttctgta tatctggcta agaaataatc ggataatttc attacagtcc
    12181 ccctttgata gtaccctgta ttttattaca ttttaatact attattaaca aagtatgcta
    12241 tgaataattg caaacacatt ttacattata ttttcaaaaa gcaccaacta tatagcaatg
    12301 atgtttttat ctctaataat aattatcttg actttatgcc tgaaaaaatg gacaatcggg
    12361 ttgtatacta ttgtagcaac gaatgggtcc accccgttat ggctggcata tttgtcggca
    12421 gtcatcctga atcatgtcct gaactggttt caggatcatt tcaggatttt agttaattat
    12481 ctgtattccg ggtcaagccc ggaatgacac tgtggcaaca aatgtcattc tgagcgttag
    12541 cgaagaatct tgccattcaa aggatagatg ctccagtcac taacgcttcc tcatcatgac
    12601 acagtatgct attttaggag gatatatcta tgaaaaaaat attgatgatt ttttcatttg
    12661 ctttattagt agtgtatgcc aactcaataa tcattgcaaa aagtcaaagg gaggttacat
    12721 ccatggtaga cgcaatacac tggcttggac atgatacctt caagattgta aaggatgttg
    12781 ttatctatac cgatcctttt aaaataaaaa acaaggataa tgctgatatt atccttatta
    12841 ctcatgaaca ctttgatcac tgctcacccg atgatattaa aaaactgcaa accaatgata
    12901 ccgtcatcat tgcaccaaaa gattgcatcg caaaactttc aggcaatgtt aaagctatca
    12961 ctcccggcaa aagcataaca gtaaaaggca taaccattga agctattcca gcatacaata
    13021 ccaataaaca atttcaccct agaagcaatc agtgggcagg atatattatt actatcgata
    13081 ccatgcgtat ctatatggca ggtgatactg attatatccc tgagatgaaa tcgctcaaga
    13141 atatttctat tgcgctgctg cctgtatcag gcgtctacgt tatgactgct gacgaagcag
    13201 cgcaggctgc attgacaata cagccggcag ttgcaattcc catgcactat ggctcgatag
    13261 ttggaactat cgccgatgca aaaaaatttg aaagccttct taaaggcaaa gtgcaggtgg
    13321 tgataaagaa acaagaaaat taaggtgtgc gtaatttttg gcgtgtatgc ctttttgtag
    13381 tgtaaacgcc aaatcccctg tgctacagga ataataaact acgataggaa gagacgagcc
    13441 tcccgatgtt acagcaacgg ttagcggcat gtgctttttg tgagcgtatt gatgttttat
    13501 tttgctctgg cgtacttcat cggcaatccc cagtaaaatc ggcagcaatg caccccttca
    13561 tctatgcaaa aatcaaacat atagctacga tttatttcat atcgtagata gttatcggta
    13621 tttcctcttt ttacttcttg atcaatttca tttaaaaaag ctaaaaactc atccaataca
    13681 ttttccatgc aaaactcaat aaccttagct acgctcatct tccagacctt ctttacatca
    13741 agtaaatatt catattcgcc atactgtaga tatagatgga ggcgtttcca tggattgtcc
    13801 ttttttctat ctcgatacga aatgctttta aatgcctttg atggagcttt ttctttttta
    13861 gcagcataga ttatcatata cacaataaat gaacgtatgg acatatcata ctggtcagcc
    13921 aaccttgcaa tacaatgtaa atgattatgc gatacacaag ttgtagtttc aaaatacatg
    13981 atatcctcct tccatagtat actttttcat ttacgatagt ttgcctggca aaaaaatgca
    14041 agaaaaaatt gtaagtacat tccatattct atcaagacaa ctattgatta tagttgactt
    14101 cttttagcgc tatagtttat tagtcataat gagcaaccgc tcatttttaa gaggcaatta
    14161 tgcaaccatt aaaaaagttc attgcaataa tcattctgtt catatcttcg agcgctttaa
    14221 ttcaatgcag tacaagcaca aagataacca tacactattc cccggcacat tatgaattat
    14281 caaactcctt ccttgccggt gcaagcaaag cagatataac ccctccgcct ggtaaacccc
    14341 tggcaggata ttcttctaat gcaaactttg ctaaaggttt caggacgcgt ctctacgcac
    14401 gggtaatcta tttgaagtca ccattgcata atgctattgc acttgtacaa tgcgatttgc
    14461 tgtcaggctc aagtttggta caacaaaggg tagcagagct tgtagcttca acaacagatg
    14521 ttgcctataa cggcattgta atagcaggca atcatacgca ctccggacct ggtaattatt
    14581 ttgaaagcaa tttttacaac cgatttgcgt caaacgcttc tggttttgac gaagattatt
    14641 ttaatttttt atgcaatcag attgcacaag caatcattga agcctataac aatcgtaagc
    14701 caggcatgat tgcctatgga gaaaaaacat tatatggatt tacccgcaac cgaagcatta
    14761 ctgcatatac cgcaaatgcc aatgcaaact ccagcaatcc tttgaaagcg gtaaatccca
    14821 tactgtctat gatccgaatt gatacagttg aaaatagatc aatccatccc attgcagcgt
    14881 tgagcatctt ttccatacat gggacagccg ttcctgatag caatgaccta tacagtgctg
    14941 atgtctttgc ctacattgag cgggaactgg aattggagat gcagcgtaac ggtgccacaa
    15001 ccttcatcca tgcagtggtc aatggtactc atgccgacaa tgctcctaac ataatgaagg
    15061 gcaaacaggg ctttcaggaa gcccgtcgga taggtattca aatagggcac catgctattg
    15121 aactttttaa tgagctatcg gctaaacttt cctctcatgt tgatagcaaa agcgttatac
    15181 aatttattga tgtgtatgca catcctaccg ttgatggtgc tacactatgc atccctccca
    15241 aggtaggcaa tacactcctg gcaggagctt ccgatggtgg tcctactccc attatcagca
    15301 gtactccttt ttttatggaa ggatcgcgcc gttttatttt tacctgcggt tgccaaaaaa
    15361 ataaacgcat tgctgctggt cctttgcaac cactgatact ccccggcgat gattttcctc
    15421 atatactgac gtttcagtca attgcaatca atcagctttt attgcttcct gttccttttg
    15481 aggtaacctg tgaatcagga aaacgcattg ctaatgccgc acttactgca tcgcctcata
    15541 ccggaattgt tactccaatc gtcattagct gtgcaaatgg atacacaggt tactgcacca
    15601 cacccgagga atacgccaaa cagcgttacg aaggaggtca tacgctgtat ggcccctcaa
    15661 cacaaccata tctagcaaaa cagtatgccc aattgtcagt aactatcgca caaaatagta
    15721 tgtccgatag ttcctggact aattgctatg tgttaaagct tgcctcattt tataaaaaat
    15781 atcccatgcc caccaccatc cgtcgggaag cattgagcaa accggtatat gtgtgcaacc
    15841 ccggcataga tgaaacctat gttgaattta cctggagcga tctgcctcca gactatatgg
    15901 aatttcacca atcgctggta agcattgaat acagcaccga tggcatacag tttcatccgt
    15961 ttataaaaaa tggcatcacc gttgatgaca ccggctacga catcgccgtg ttgtttaccg
    16021 gcaccataac aaaagagggt aatgcgctct atacaacacg ctggtataac cctgagcttg
    16081 ctgaaaatgt gtattttcga tttgcaattc agccgcgtca aaagctcccc tttgtatact
    16141 cagagccatt tagctggtag cttgctatta gataactgtc aaaaggggat ttttcgctat
    16201 cactcagaat aactatcgtc tttgcaccaa aatgacattg ttgatgcaat tatcacaggc
    16261 actgcattcg ccagctattt tcatctttca catttttcat cttgcattgt gcacccattt
    16321 gcaactcccc ctatcctttt tttgagagga ggccgggtgt gaggctatca ccggtaacta
    16381 tcgccctgga tattattact gttaccaatg acaataatta taaaatagta taaaataatt
    16441 gacattgcaa ttcatgtacc tgtacaatct ctatgaatta atgtaatgcc gtaacctttt
    16501 attacagggg aacatctatg acactaaaaa ataagattct tattatttta agcctgtgcc
    16561 ttttactatg cattggagca tattttatta tctcacgtac ctttgccaat tttgaaaagc
    16621 agctctttga aaaatgccgc attgaagcac tggttggcgg gcgtgttatg agtgaaatca
    16681 ttgatatgat gattgacacc cgtcttctta ccaaacagca ggtatttgac cgcaattatg
    16741 ttcccattca aggaagcaat cctcagaaat tccgaactca atatgatgct gtctttgaca
    16801 aatatattca aaaaattgaa gatgaatttt taaaagataa agaccttgaa tttgctgtac
    16861 tcgttgatat caatggatat cttcccacac acaacagcaa atatgcaaaa cctgaaacct
    16921 ccgaccctga tcacaacctt aaatacagcc gttctaagcg catctttaac gatagggtag
    16981 gacttaaagc atcccgtttc attggtcctg gtacattaaa acagctctat actcgtgata
    17041 cgggtgaagt tatgtgggat atatcatccc ctgtgtttgt caagggagag cattttggag
    17101 cattcaggct cggagtatca ttaaaacaca taaatgaact gaaaaatcag atgatcatta
    17161 ttgttggcat gaccatactg gtaatactca gtatcaccat gcttatgctt tttttaatac
    17221 tgccgcgaaa actgtacgat acagaccttg acataccgca atattaacgc tgcccttttt
    17281 tttgtttgtc aatctcaatc tgagcatatg ccgagtggtt atgtattgat tccatgcttt
    17341 ctgtttctac ctgaaaccac aaaacctttg gatgagctaa cagtttaata gctacattcc
    17401 tgacaatatc ctcaacaaat acgggatttt cgtatgaacg ctcggtgatg tacttttcat
    17461 cctcgcgctt aagcaatgca tatatttcac tggagccaca gtgttcaaca atctctataa
    17521 attcttctat ccataaaatt tcttttgccc tgacagttac acagacaacg ttgcgttgat
    17581 tgtgcgcccc atagcggctt atctctttag aacatggaca aactgataat aatggcactt
    17641 tcacctgcag cgtataatag tcaaccgatg aatcaccctg caaaatacaa agataatcca
    17701 tcattgattt ttcaccactg acaggagcag cctttactat aaaatagggg aattcaatag
    17761 ttacatgcgc agtttttgca ttaagctcgc cttttaaatc atcaaggatg ttccgcacat
    17821 ttttggtgct tataccgccc tgatagcggt tcagtacctc aacaaaacgt gacatgtgcg
    17881 ttccacggaa atgctcgggt aaatcaacat acatgtttat acgcgcaacc gtatgctgca
    17941 atccatcggc tctgtccatc acaactaccg ggtaggtgat atccttaaca ccaaccttat
    18001 tgatgggcag gttccggtca tccggctgtg attgtatatc tttaagactt tttcgaggca
    18061 taccattact tcatgctttc acttcttctt gtgcagtcag ccctgcctgc tgtttaatct
    18121 tttcttctat ctcttttgca ctatcaggat gctgatctaa ataacttttt gcattttccc
    18181 gtccctgtcc tatgcgctca tccttatacg aataccatgt gccagccttc tgaataatat
    18241 tatacttaac gcctaaatca agtatacccg ccgtacgcga aataccgctg tcatacatga
    18301 tatcaaactc ggcctgacgg aacggtggcg acaccttatt cttcacaacc ttgacgcgca
    18361 cccggttacc aaccgcatcc tcgccaacct taatagtatc aaccctgcgt atatcaaggc
    18421 gtaccgaagc ataaaattta agcgcattgc cgcctgtagt ggtttcgggc gaaccaaaca
    18481 taactccaat tttatggcgt atctgattga tgaagacaac gcttgttttt gattttgcaa
    18541 taacagcggt aagcttccgc aacgcctggc tcatgagtcg cgcctgcaat cccatatgcg
    18601 catcgcccat ctcgccttca atctcagctc gtggcaccag tgcagcaacc gaatcaatga
    18661 caacaatatc tatagcccct gagcgcacca gtgattcagt tatctccagc gcttcttctc
    18721 cggtatcagg ctgcgacacc agaagctcat cggtgttgac acccagcttt tttgcatagg
    18781 taggatcaag cgcatgttca gcatcaacaa acgccgcaat gcctcccaat ttctgagcct
    18841 cagcaatcac atgcaatgtc agcgtggttt tgccggatga ctcgggacca taaatctcgg
    18901 tgattcgccc tcgcggtata ccgccaatcc ccagcgcaat atcaagttca agcgaccccg
    18961 ttgatatgga cggcaccttc atcgacgcat gttcatcgcc aagccgcatg atgga
//
LOCUS       DAOSOX010000026        18918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2927_length_18918_cov_9.086572, whole genome shotgun sequence.
ACCESSION   DAOSOX010000026 DAOSOX010000000
VERSION     DAOSOX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2927_length_18918_cov_9.086572"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLX22_03405"
     CDS             <1..375
                     /locus_tag="PLX22_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOT18982.1"
                     /translation="QGRYQSIDMDEDFNFFIMDDDVQYPLSRFSGGEIDCANLCLRIA
                     ISNAIRDFSGGGAVGFMAFDEIFGSQDNDRRAAIMDVLYRLQEHYRQIFIISHIDDVK
                     ELFPSILHIQNSGTGSTATWLA"
     gene            551..808
                     /locus_tag="PLX22_03410"
     CDS             551..808
                     /locus_tag="PLX22_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18983.1"
                     /translation="MKTERFIGVESLRMTMLEYVMLRWQLHGTCKEILIALDNYKNGE
                     NQYRMTQNACYYYNYEQYYQLVRFNSTKKWYGGDPERIVTA"
     gene            946..1872
                     /locus_tag="PLX22_03415"
     CDS             946..1872
                     /locus_tag="PLX22_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT18984.1"
                     /translation="MVTRKSFLNNIKDLLMAIPVAGLPWLKNIYAKDMVSSRDLVVVK
                     AKTITDASIAAMVKVGLQEMGGIERFVKRGSKVIIKPNIAFNSTPDRAHTTNPSLVET
                     VARICKNAGAQVTIMDRPVHNARLCYHSSRIEEAAKRAGVNCEYMNRDRFKTVHVPQG
                     LNLDTVDVYEEILEADCIINMPIAKHHNAAMVSLSMKNLMGVIGGNRGFYHVNLHRNI
                     VDFNKAVKVHLIIMDGLRILTKHGPSSGGPEDIKETKTIIFGINPVMVDTYTAHYLFG
                     IEPSSIGYLSLASNAGMGSINVNTYSVITKTV"
     gene            1869..3362
                     /locus_tag="PLX22_03420"
     CDS             1869..3362
                     /locus_tag="PLX22_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT18985.1"
                     /translation="MKSKHWSLFRAIIQLAFWLIFVGSIVYLHDPLSDVRYGLYPSLS
                     IYSIFIGSITGHTLKYAFLGIAIALLTVLFGRFFCGWICPLGATIDASDTVIETKAKN
                     KQLKKITRIKILILLATLLIAFLGFNATGYSDPITIAYRGYELFLYPFISYLGSEPLS
                     WVLPGYAARDYPFYYYNWIWPAIIFIGILALSLFFKRFWCRVLCPLGAIYALLSHFSL
                     LRRTVDADKCVHCGACVKGCRMGALSSDGTGTIEHECIKCFDCLKHCNYGAIQFRFLP
                     PYKSKSIAHPAQGITRKDLLVVAGSSLMIASTRLTLFAASHESYIIRPPGALPESDFC
                     NACIRCGQCMNVCPTNALHPCMIEAGFYGMFTPRLVSRIGYCDFSCTRCGTICPTGAI
                     QKLSLAKKQTFVIGTAYIIKDLCIPWSESTNCIVCEEVCPVQNKAITLDRKVITNKKG
                     EAVEVLLPVVHTELCIGCGICENRCPVSGGGAIMVRKPKLTMGDSYG"
     gene            3355..4263
                     /locus_tag="PLX22_03425"
     CDS             3355..4263
                     /locus_tag="PLX22_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT18986.1"
                     /translation="MANEKHHEAYVARKVTWIGMIVNIMLSVFKLVAGIIGHSQAMVA
                     DGVHSISDLTTDAAVLIGIRYWSTPADEDHPYGHERIESLVTAFVGVALFIVGIGIAY
                     NGIVTAFEPDIKSPELIALIAAFVSIVSKELLYRWTVKLGKEIDSSSVVANAWHHRSD
                     ALSSIPALLAVGLAMIHPKLAFFDHLGAVVVAIFIIKVSIDIMKPVIMELSDKGAPKP
                     QISQIDSIVMGVEGVKEVHSIRSRRCGSGYFVDLHVLVDGNMSVQSGHDIARTVKYQL
                     LDKGPKVLDVMVHLEPYQTSSKKNST"
     gene            4260..5036
                     /locus_tag="PLX22_03430"
     CDS             4260..5036
                     /locus_tag="PLX22_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOT18987.1"
                     /translation="MKEYSVTIRFYEELNDFLPANKRKVAFTVSWKGKRSVKDLVESL
                     GVPHVEIDLIVVNGVSVGFDYIVQNDDYISVYPVFEQFNINHITQLRPHPLRHPTFIC
                     DVHLRKLVKYLRLFGFDVLYNEQWDDKDLAQIAAEQKRILLTRDRQLLMRKIVDRGLI
                     VRNILPQKQIVEILNRLDLWDMVTPFSRCIMCNNHVRQLLINDHSFDNEKKKIPIGVL
                     QWCTVYYKCLGCGRIYWEGSHYERLTKKIKTILSEKNKRS"
     gene            5324..6961
                     /locus_tag="PLX22_03435"
     CDS             5324..6961
                     /locus_tag="PLX22_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT18988.1"
                     /translation="MKILLGYCVEKEHYHDYYVTLLPVGLVSIASYLSNSGYDVVLAN
                     FSKKSTEQIIKEITHIKPQLIGFSLFTHNRSITLYIVNKIKILFPNSVIVLGGPHATF
                     LDKEILQRFPSVDCIVEGEGEAAMLAIIKNIEQGKPPRHEIINMHSVNDVSNIPFPSQ
                     FNGTLYNINVNEQYKYIITSRGCYNNCTFCDSPAFWGKKVRFREVDDVIEELETIYRK
                     YGIIYFSIRDDNFTVKKDRVQELSYKMIRKKLYMMWNCQARVDAVDESMLIAMKKAGL
                     EHIQYGVESGSEKILALYNKKTTIDTIIHAANITRKVGIYLSIYLMVGMKGEDYNDIK
                     KTKKLLASILPGDCIVSPVALYPGTKLYEEEKKNGALSDSIWFNKNDAGIFLRNEPVI
                     KEWMNELLMATYALRDKAWYKAKDFAMHKKIAGDCWVTDILEGDYYSDNELYPEAATV
                     YGNVIKNIPDNPWGYMRYGKLHFIIGDYETAMKSFLEVTKIVPNYYGGWLKFAQSCIA
                     LGLNEKAKQAINKALQLNPYDERINALNSLLKNNRRK"
     gene            7076..8047
                     /locus_tag="PLX22_03440"
     CDS             7076..8047
                     /locus_tag="PLX22_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOT18989.1"
                     /translation="MAQNNIHDKAYKYLFSNPLIVKELLESFVAMDWIKQVNFNKAQP
                     IDKSYVNDDYKEYEADIIYKLYFKDRQLYLYLLIEFQSTVDRFISFRMLNYVMELYRE
                     LIYKQKLKKLPVVFPILIYNGDRKWTAPQSLQELIDMPESLKQCTAYIPQFQYYPIIE
                     NELSRKTLESMMNVISTVFLFETGDTMVFLKTVDRIKELLHIYNDEPLMLRTLFYWLM
                     HYLQTHGKIDDAAVILETIEEPEEVHSMLSKTIQKMQEDFRKEGFHEGIHIGIDKGIQ
                     KGINEGISIERRNIAINMLKKGIDKKTIIEITKLNKKQLADIEKGMQ"
     gene            8146..9429
                     /locus_tag="PLX22_03445"
     CDS             8146..9429
                     /locus_tag="PLX22_03445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT18990.1"
                     /translation="MIANKNQVAAINAEQGAHLVIASAGSGKTYTIIQRSIALINKKL
                     CNPDEILLLTFSRKAADELKQRIVAVLGNAGSRVVAATFHSFCLQFIIKQTMNDIKVL
                     DEDISQNIMKEIINNHIAELYGIPSMVVYKIVSKLQTNRISQLHGDLYAKMIMIKEEY
                     ASYKRNHNYIDFDDMINISSKLLHENPLLRKKIHEQFKYIMVDEFQDTSENNFVLLKL
                     LLPEEKQNVFMVGDDWQSIYKFRNAKIEYIVNAKKYFNNLTVHSLNSNYRSKKEIVTL
                     SNRLIAKNKFRSRRWIKAVRGNGGLILFHKASSFAEEVTITASILEKYDSTHLIGVLY
                     RNNWQGTYLQSQIQSYGNVQFMTIHSAKGLEFDVVVLCGVKDRLLPDPYTDIEEERRL
                     MYVALTRAKNCLHILYHCNYGNQKPQFVMECERCF"
     gene            9511..10395
                     /locus_tag="PLX22_03450"
     CDS             9511..10395
                     /locus_tag="PLX22_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT18991.1"
                     /translation="MDYILEVNNLYKGFPGVKAVDGVSFSIPYGICFGLLGPNGAGKT
                     TTIEVIEGIQKPDEGIILYKGKNRDHRFKQEVGIQLQATELQQYLTVKEILLSFRNLY
                     AKRADLNDLIAMCYLDEILNRDNRKISGGQKQRLLLAIALANDPELLFLDEPTTGLDP
                     QARRHVWDIVQTVKMKGKTIILTTHYMEEAQNLCDVVAIMDQGKIIAMDSPINLLNQY
                     CSGVTITLPSVIDVDMLNSIECNWYMVNNTIEIQTEKPHDCLRVLIDKNIDITGMVVR
                     SQNLEDLFLKLTGTQLRA"
     gene            10402..11430
                     /locus_tag="PLX22_03455"
     CDS             10402..11430
                     /locus_tag="PLX22_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT18992.1"
                     /translation="MLYRIWTIFVARTKEFYRDRSALGWNVIFPLLIIVGFSVVFRSD
                     SSPILKVGIVALAEKPAENGISNIQFNEFLKIKYINFIQFNSKEKAIGSLQKFKIDMV
                     IDSNSKTYWINESSPNGYLAEQLLKGAQNKSSVFKKQSFYNKGVQYVEWLFPGILGMN
                     AMFNSLYGVGYVLVMYRKNGILKRFSVAPVRPFEFLSAQILSRMFVVLATTVVVYVGT
                     VLLYGFKCKGSLFTLFILFALGSFCMISLGLLIASRSDSQEFADGIINIITWPMMFLS
                     EVWFSLEGSRPWVQKVSQFLPLTHIVDGARMIMNEGATLFDLQLQIVYLVAISLLFLF
                     LGSLLFRW"
     gene            11545..11616
                     /locus_tag="PLX22_03460"
     tRNA            11545..11616
                     /locus_tag="PLX22_03460"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11577..11579,aa:His,seq:gtg)
     gene            11629..11702
                     /locus_tag="PLX22_03465"
     tRNA            11629..11702
                     /locus_tag="PLX22_03465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11663..11665,aa:Arg,seq:tcg)
     gene            11723..12364
                     /locus_tag="PLX22_03470"
     CDS             11723..12364
                     /locus_tag="PLX22_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18993.1"
                     /translation="MNSTVKDFDIFNYLPKEEDSVINNPYLQEILTYRNNQFDEYQYW
                     NVRYDLIKKYAFAVPTIDTIKIIQELSPIIEIGAGNGYWAYILNQVKVDIIATDKYPP
                     DETALPFSNYSSNQWFHNQWYDVIKAEAIEAGNHPERALMLCWPEPQSMMAYESLYAY
                     KNAGGHTIIFIGDMIACAESNFYDALAQCKLEVKQQIWGYKYFNEIIAIYKIY"
     gene            12450..13184
                     /locus_tag="PLX22_03475"
     CDS             12450..13184
                     /locus_tag="PLX22_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOT18994.1"
                     /translation="MIEAHKKILIVSMPMVDSDLKLYLAPSRCIDSDDPLVVDFAHSA
                     IGSEADAVKKSVKLFYAVRDNIYYDPYSPFYKPEHYHGRFVLQRGRGFCVSKASLLCA
                     LARACNIPSRVGFATVKNHIATKQLLETLGTNVFAYHGFTEFYLNKKWVKATPAFNKE
                     LCILHKVSPIEFNGVDDAIFPEYNSENKLYMEYLDYHGSFYDVPVETIITAWKECYGS
                     ERVELWITLFEQNFANRKFEGEEIVK"
     gene            13188..16700
                     /locus_tag="PLX22_03480"
     CDS             13188..16700
                     /locus_tag="PLX22_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT18995.1"
                     /translation="MQIVPIIDILKFIDCDFICVDSLLEIIHSNNDSFINKNIADTFP
                     YSLIIDDVKRYCLELSTNAGVKTVVKFQNENCLYQFSLFDSKYGDKTIMVIIAKPKKQ
                     IPEVIQDICHNLFKVKIADDLQHFVDVFEYILNSIIQVTGMDCGGIYIIDQLTMKLKL
                     TFARGLTNSYIKSASIYNNESWNYQLVMNGTPQFIDYTNMELCDNDFRRLEGIKAVAI
                     VPIKFRNSIIGVINVGSHSTNTISQNIYPLLEVFGEQLGTIMDNYMVKSVLAQSKYKK
                     EIIDTIHDVVFQLDKDGIVQYMSGNISTITGFTPDEILGKYFINFIYPEDVAMFIESC
                     ELAKRRIFEPLEYRLITKEGSYRYILTISESHYDEKGNYIGLTGVVTDIHDKKLAEIK
                     LKESDERFKTLASSVAAGIFVINDFRVYYVNPAVIQILEYPEEEILGKLFWEFISEED
                     RGWIIERAKARLEGKSVPTRYELRLVTRNNTIKWVDFSATQIMLNGKPATLGSVFEIT
                     EKKRIESELKQSEEKFYKAFHSSPIPMTINTYNDGMYREVNEAALTTFGFSLQEALGK
                     SIYDLDVFADKNELKQYLQELRTNREVKDMDILFQTKNGKLKQCSINSDIFISSKEEF
                     VVSLIVDITQIKQNAQIILDQFNELQKINADLANVQAKITTQNIQLSEEKELLNRILE
                     SITDAVIVTDSYGKIVLVNTITCSILGKPANLILNTMASDLLTIECKQSVSLNFKEIY
                     DTLKNNSIHQECILRVNERKYDIEFNLLPLKNQHNDVNGAVILLRDITQKKIVEQQMA
                     KTSKLESTGLLAAGIAHDFNNILTSIVGNLSIVKYKINENSELYNSIRGAETAAFKAK
                     ELTQQLLTFAKGGSPLKKLTSLKNILVETSTFVLRGTAIEFRFIIADDLYNVEVDEAQ
                     IGQVIQNIVINSREAMQGYGVLKIAAHNVIENDPVRVIIGNELYVQISIEDNGVGIPR
                     DILEKVFDPYFTTKPHGTGLGLAVVYSVIKQHGGYIFVDSEAGKGSRFEIYLKASKNK
                     ATLTEIPYCDSITVTGCGKVLILEDDSNIQFILKTMLEELGFEIDITIDGEEAFYKYM
                     NAKYNNNKYAFAIMDLTIPGKQGGKDTIRQIRAIDKDFKVIVSSGYSNDPVMSNYSEY
                     GFNAILPKPYRLEDLKKVIATIMA"
     gene            16886..16958
                     /locus_tag="PLX22_03485"
     tRNA            16886..16958
                     /locus_tag="PLX22_03485"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16919..16921,aa:Lys,seq:ctt)
     gene            17027..17108
                     /locus_tag="PLX22_03490"
     tRNA            17027..17108
                     /locus_tag="PLX22_03490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17061..17063,aa:Leu,seq:tag)
     gene            17113..17184
                     /locus_tag="PLX22_03495"
     tRNA            17113..17184
                     /locus_tag="PLX22_03495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17145..17147,aa:Gly,seq:gcc)
     gene            17499..17571
                     /locus_tag="PLX22_03500"
     tRNA            17499..17571
                     /locus_tag="PLX22_03500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17532..17534,aa:Thr,seq:tgt)
     gene            17577..17658
                     /locus_tag="PLX22_03505"
     tRNA            17577..17658
                     /locus_tag="PLX22_03505"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17611..17613,aa:Tyr,seq:gta)
     gene            complement(17783..17992)
                     /locus_tag="PLX22_03510"
     CDS             complement(17783..17992)
                     /locus_tag="PLX22_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOT18996.1"
                     /translation="MKTSITKDMTFGELVQKYPVAASILASYGLHCIGCHIGIYETIE
                     QGSRAHGLTDAQINEMLEKLNNTIK"
     gene            18274..>18918
                     /locus_tag="PLX22_03515"
     CDS             18274..>18918
                     /locus_tag="PLX22_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT18997.1"
                     /translation="MKQQIKNRKTIKTRIIIYCIICLIILYKHINVLADSYANASLSD
                     CLQIALQNNISIKIAFEEVNGALSNVKITKGSNNIQVNGELRTIEILKSDAANSSFNV
                     PGKDTLIGLFIGASATYKLYDANKSNYEQLKFLEYNLAILSALKIKNNVMRDVKITYY
                     QLLLAKKKSELLEKLVKSFEIKYLNAEQLFTSGQRTMVDVSKAQVDLTNARLKYE"
BASE COUNT         6382 a         2815 c         3758 g         5963 t
ORIGIN      
        1 caggggcggt atcaatcaat tgatatggat gaagatttta atttttttat catggatgat
       61 gatgttcaat atccattaag cagattttcg ggcggtgaga tagattgcgc caatctttgt
      121 ctgcggattg caattagcaa cgctatcaga gatttcagtg gaggcggagc cgttggtttt
      181 atggcgtttg atgaaatctt tggcagtcag gataatgata gacgtgcagc cattatggat
      241 gtgctataca ggctgcagga gcactatcgt cagatattta ttatctcgca tatagatgat
      301 gtcaaagagt tgttcccttc catactgcac attcaaaata gtggtactgg ttcaacagca
      361 acatggcttg cgtagaatgt tagcaatgta tgcactattg tttttacaaa tataatgaaa
      421 aattttggcg atagttatcc tgaatcatga aatcggtaat gagggaagta aaagcaaaga
      481 attatattgt gccgtgtcat cctgaacaaa gcaattgtca ttctgaacga agtgaagaat
      541 cttgtctttt gtgaaaacgg agagattcat cggggttgag tccctcagaa tgacaatgct
      601 ggaatatgtc atgctgagat ggcagttgca tggaacatgt aaagagatac tgatagcatt
      661 ggacaattat aaaaatggtg aaaatcaata tagaatgacg caaaatgctt gctattatta
      721 taattatgag caatactatc aattggtaag atttaacagt accaaaaaat ggtatggagg
      781 cgacccggag cgaatagtta cagcatgaaa aataaagctt gtattggatg gtagtcatca
      841 taacgtttca atggtaaatg cgaataagga aaatgtgctc tgtaactttt gatggtaaaa
      901 atagtatgtt gtgtgaaaca gtataataat actggagagg attatatggt aacacggaaa
      961 tcattcttaa acaatataaa agatttattg atggctattc ctgttgcagg attaccatgg
     1021 ctgaaaaata tttacgcaaa agatatggta agcagcaggg atttagtagt tgtaaaagct
     1081 aaaactataa ctgatgcatc cattgcagcg atggttaaag taggtttaca ggaaatgggt
     1141 gggattgaac ggtttgtaaa aaggggtagc aaagtaatca tcaaacctaa tatagctttt
     1201 aattccacac ctgacagagc acacaccacc aatccttccc tggtggagac tgtagcacga
     1261 atatgcaaaa atgctggagc gcaggtaacg ataatggacc gcccggttca taatgccaga
     1321 ttatgttacc actcaagcag gattgaagaa gcagcaaaaa gagcaggtgt taactgtgag
     1381 tatatgaaca gggacaggtt taaaacagta catgtgcccc aggggcttaa tttagatacc
     1441 gttgatgttt atgaggaaat actggaagct gattgcatta tcaatatgcc aatagccaaa
     1501 catcacaatg cagccatggt atcactttca atgaaaaatc tgatgggcgt cattggaggt
     1561 aaccggggtt tttatcatgt taaccttcat agaaatattg ttgatttcaa taaggctgtt
     1621 aaagttcatc ttatcatcat ggatggattg cggattttaa caaaacatgg accatcatcg
     1681 ggaggtcccg aagatataaa agaaacaaaa acaattatat ttggaatcaa tcctgttatg
     1741 gtggatacat atactgccca ctatcttttt gggatagagc cttcatctat tggttattta
     1801 agtctggctt caaatgccgg gatgggaagc attaatgtta acacatattc tgttataaca
     1861 aaaaccgtat gaaatcaaag cactggtcat tattcagagc tataattcaa ttggcttttt
     1921 ggcttatttt tgttggcagc attgtttatc tgcatgatcc cttatctgat gttagatatg
     1981 gattgtatcc ttcactatcc atatattcaa ttttcattgg ctctattaca ggtcatacgc
     2041 tgaaatatgc atttctggga attgctattg cgttgctgac agtattgttt ggaagatttt
     2101 tctgcggatg gatttgtcct ttaggcgcta ccattgatgc ttctgataca gtgatagaga
     2161 caaaagcaaa aaataaacag cttaaaaaaa taacgcgaat taagattctg atacttttag
     2221 caacactgct gatagcattt ctgggattta atgcaacagg ttatagtgat ccaataacta
     2281 tcgcatatag aggatatgaa ttatttcttt atccatttat ttcatatttg gggtcagagc
     2341 cattgtcgtg ggttttgcca ggatatgctg cacgcgatta cccgttttat tactataact
     2401 ggatatggcc agcgattatt tttattggga ttttagcatt gtcattgttt tttaagcgat
     2461 tctggtgcag ggtgttatgt ccgttaggtg ctatctatgc gctgctatca catttcagtt
     2521 tattacgacg aacggtagat gctgataaat gtgttcattg tggtgcatgt gtaaaaggtt
     2581 gccgcatggg agcactatcc agcgatggta cgggaacgat agaacatgaa tgtatcaaat
     2641 gttttgattg tttaaagcat tgcaactatg gtgcaataca gttcaggttt cttccgcctt
     2701 ataaatcaaa aagcattgct catccggcac aggggataac acgaaaagat ttgcttgttg
     2761 ttgctggttc atcgttaatg attgcttcaa cacgcttaac actatttgct gcatcgcatg
     2821 aaagttatat tatcaggccc cccggtgcac ttccagaatc tgacttttgc aatgcatgca
     2881 tacgctgcgg ccagtgtatg aatgtgtgtc ctaccaacgc attacaccca tgcatgattg
     2941 aagcagggtt ttatggaatg tttacgcccc gacttgtttc gcggattgga tactgtgatt
     3001 tttcctgtac acggtgtggc actatatgtc caacaggtgc tatacagaaa ctatcgcttg
     3061 caaaaaaaca aacatttgtt ataggcactg cttatattat aaaagatttg tgcataccat
     3121 ggtcagaaag cacaaattgt attgtttgtg aagaggtttg ccctgttcaa aacaaagcaa
     3181 taacgcttga caggaaggtt attaccaata aaaaagggga ggcagttgaa gtgctattgc
     3241 ctgttgtaca tacagagctg tgtatagggt gtggcatttg tgaaaaccgc tgccctgttt
     3301 caggaggcgg tgctataatg gtgcgaaaac caaaactgac tatgggagat agttatggct
     3361 aatgaaaaac atcatgaagc atatgtagcc cgtaaagtga catggattgg aatgatagta
     3421 aatatcatgc tttctgtttt taaacttgtt gccggtatta ttggtcatag ccaggccatg
     3481 gttgccgatg gggttcacag tatttctgat cttactaccg atgctgctgt acttatagga
     3541 atacgttact ggtcaacacc tgcggatgaa gatcatcctt acgggcatga acggattgaa
     3601 agccttgtga ctgcgtttgt gggtgttgcg ctttttattg ttggtattgg tattgcatat
     3661 aatggcattg ttacagcatt tgaacctgat ataaaaagtc cggagttgat tgcacttatt
     3721 gcagcgtttg tttctattgt atcaaaagag ttgctatacc gatggacggt aaagctgggc
     3781 aaggagattg attcatcgtc agtggtggca aacgcatggc accatcgctc ggatgcttta
     3841 agttctatac cggcactttt agcagtagga ctggcaatga ttcacccaaa attggctttc
     3901 tttgatcatc ttggcgcagt tgtggtggca atctttatca tcaaggtatc catagatatc
     3961 atgaaacctg ttatcatgga actatctgat aaaggtgcac ccaaacctca gatatcacag
     4021 attgattcaa ttgttatggg tgtagaaggt gtaaaggagg tacacagtat acgttcgcgg
     4081 cgatgcggca gcggttattt tgttgatctg catgtgcttg tggatggcaa tatgagtgtt
     4141 cagagtggac atgacattgc acgtactgta aaatatcaac tgctggataa gggcccaaag
     4201 gttctggatg ttatggttca tcttgaaccg tatcaaacct catcaaaaaa gaatagtacg
     4261 tgaaggaata ctcagtaaca atcagatttt atgaagagct gaatgatttt ttgccggcta
     4321 ataaacgcaa ggttgctttc acggtaagct ggaaaggcaa gcgcagtgta aaggatcttg
     4381 ttgaatcact gggtgtaccc catgttgaga ttgatcttat tgtagtaaac ggcgtttcag
     4441 taggatttga ctacatagta caaaatgatg attatatcag cgtataccct gtctttgagc
     4501 agtttaatat aaaccatatt acacaattac gccctcatcc tctgcgtcat cccacattta
     4561 tttgtgatgt gcatctgcga aagctggtaa aatatctcag gttatttggc tttgatgtcc
     4621 tctataatga gcagtgggat gataaagacc ttgcacagat tgctgcagag caaaaaagga
     4681 ttttgttaac tcgtgatagg cagcttttaa tgcgcaaaat tgtggataga ggtcttattg
     4741 tgcgtaatat actgcctcaa aaacagatag ttgagatctt aaacaggctg gatttgtggg
     4801 atatggttac accattcagc aggtgtataa tgtgcaataa tcatgttcga cagttactga
     4861 taaatgatca ttcctttgat aatgaaaaga aaaagattcc cataggtgta ttgcagtggt
     4921 gtacggttta ctataaatgt ttgggttgtg gcagaatata ctgggaaggg agccactatg
     4981 aaaggttaac aaagaagatt aaaaccatac tttcagaaaa aaataagaga agttgattga
     5041 ttcaaaaaaa gcgagattct tcatctttgc ctcagaatga cacatcactt ccgtcattcc
     5101 gggcttgacc cggaattcac atgtgtaacg ttaaattcta aaatgatcct gaaaccaatt
     5161 cacgacatgg ttcgggatga caaatcgtga atattttcat cacgtataga tggctataag
     5221 ccggccattg tgaactactc tgaaatagca tggtgatgga agtttcattt tttgttattt
     5281 aagaaaatca tatagtaatg cgatacaata gtaaaggggt attatgaaaa tattattggg
     5341 ctattgcgtg gaaaaagagc actaccatga ttactatgtt accctgttgc ctgtaggtct
     5401 tgtttcaata gcttcatatt tatcaaatag cgggtatgat gttgtactgg caaatttttc
     5461 aaaaaaaagt actgaacaga ttataaaaga aattactcat ataaaaccac aacttatagg
     5521 tttttcgctg tttacccata accgtagtat aactctttat atagtaaata aaataaagat
     5581 attgtttccc aatagtgtga ttgttcttgg cggtcctcat gcaacatttc ttgataaaga
     5641 gatactgcaa cgattcccat cggttgattg cattgtagaa ggtgagggtg aggctgccat
     5701 gcttgcaatc atcaaaaaca ttgagcaggg taaaccacca cgtcatgaaa ttataaacat
     5761 gcattctgtc aatgatgtaa gcaatatccc cttcccttca caattcaatg gaacattata
     5821 taatataaat gtaaacgaac agtataaata tattattaca tcacgtggat gctataacaa
     5881 ttgtaccttt tgtgattcgc ccgccttctg gggtaaaaag gttcgtttca gagaagttga
     5941 tgatgttatt gaggaattgg aaacaattta tagaaaatat ggcataatct atttttccat
     6001 acgcgatgat aattttacgg taaaaaagga ccgtgtacag gaactatcgt acaaaatgat
     6061 tcgtaaaaag ctttacatga tgtggaattg tcaggcaagg gttgatgcag ttgatgagtc
     6121 aatgcttatt gcgatgaaaa aggcaggact tgagcatata cagtatggtg ttgagtcagg
     6181 ttctgagaaa atattagcat tatataacaa aaaaacaact attgatacca ttatacatgc
     6241 tgcaaacata acacggaagg tgggtatata tttatcaatt tatctgatgg ttggaatgaa
     6301 aggtgaggat tataatgata taaaaaaaac caaaaagctt ttggcttcca ttttaccagg
     6361 tgattgtatt gtttcccctg tggcattgta tccaggaaca aaattatatg aagaagaaaa
     6421 aaagaatgga gctttaagcg atagtatatg gtttaataaa aacgatgcgg gaattttttt
     6481 gcgcaatgaa cctgttatca aagaatggat gaatgaatta ttgatggcaa cctatgcatt
     6541 gcgtgacaaa gcatggtata aagcaaaaga ttttgccatg cataaaaaaa tagccgggga
     6601 ttgctgggtt acagatattc tggaaggcga ttattattct gataatgagt tgtatcctga
     6661 agctgcaact gtttatggaa atgttataaa aaatataccg gataacccat ggggatacat
     6721 gcgctatggt aaacttcatt ttatcatagg agactatgaa actgctatga aaagctttct
     6781 tgaagtaaca aagatagtcc ctaattatta tggaggatgg ttgaaatttg ctcaaagttg
     6841 cattgcattg ggattaaatg aaaaagcaaa acaggcaatc aataaagcac tgcaactaaa
     6901 tccatacgat gaaagaatca atgcattaaa tagtttgttg aaaaataatc gtcgaaaata
     6961 aatgtatttt tttagtaaaa ttacaaattt tttcttcatt tcttttaatt ttttcctgca
     7021 aaagcgtatt aatagatagc tcaatattaa taacttgaat ccttttgagg tatctatggc
     7081 gcagaacaat attcatgata aggcatataa ataccttttc tccaatccat tgattgtaaa
     7141 agagttgctg gaatcttttg ttgccatgga ttggattaaa caggttaatt tcaacaaagc
     7201 acagcctatt gataaaagct atgtaaatga tgattacaag gaatatgagg cagatatcat
     7261 ctataaatta tattttaaag atagacagtt atatctatat ttacttattg aatttcaatc
     7321 tactgtggat agatttattt cattcaggat gctgaattat gttatggaac tttataggga
     7381 actcatctac aaacaaaagc tgaagaagct accggtggta tttcctatac ttatctataa
     7441 tggtgacaga aaatggacgg ccccacagtc attacaggaa cttattgata tgccggaaag
     7501 tttaaagcaa tgtactgcat atattccaca atttcaatac tatcctatca ttgagaatga
     7561 gttgtcacgg aagacattag aatcaatgat gaatgttata tcaactgtct ttttatttga
     7621 aacaggagat accatggtgt tccttaaaac agttgacaga ataaaggaat tattgcatat
     7681 ttataacgat gagccactga tgctacggac attgttctat tggttaatgc attatttgca
     7741 aacacatgga aaaattgatg atgcagcagt aatactggaa acgatagaag aaccagagga
     7801 ggtgcacagc atgctatcca agacaataca aaaaatgcaa gaggattttc gcaaagaagg
     7861 attccacgaa gggattcaca taggcataga taagggaata caaaaaggaa ttaatgaagg
     7921 tatatctatt gaaagaagga atatcgccat caatatgttg aaaaagggga ttgataaaaa
     7981 gacgataata gaaatcacga aattaaacaa aaagcaattg gcggacatcg aaaaaggtat
     8041 gcagtagcat accataaaac cgttaacact gtcaatacag attatcatca gaaaaataga
     8101 ttatgcatta ttacaacaat attattgtag ataaaaacat atacaatgat tgcaaataaa
     8161 aatcaagttg cagccattaa tgcagaacag ggtgcacatc tggttatagc ttctgctggc
     8221 agtggcaaaa cctatactat cattcagcga agtattgcac tcattaataa aaaattgtgt
     8281 aatcctgatg agattctttt attaaccttc agccgaaaag ccgcagatga gttaaagcaa
     8341 cgaattgttg cagtacttgg aaatgctggt agcagggttg ttgctgctac attccattca
     8401 ttttgcctgc agtttattat taaacaaact atgaatgata taaaggtgct ggatgaagat
     8461 atttcacaaa atataatgaa ggaaatcatc aataatcata tcgcagaatt gtatggtatt
     8521 ccatcaatgg ttgtttataa aattgtatca aaattacaaa ctaatcgcat atcacaatta
     8581 catggcgatt tatatgcaaa aatgataatg attaaagagg aatatgccag ttataaacgt
     8641 aatcataatt atattgactt tgatgatatg attaatattt cttcaaagct gttacatgaa
     8701 aatccattat tacggaaaaa aatacatgag cagtttaaat atataatggt tgatgagttt
     8761 caggatacat cagaaaataa ttttgtactg ttaaaattgc ttctgcctga agaaaaacaa
     8821 aatgtattta tggttggtga tgattggcag tcaatttata aattcagaaa tgcaaagata
     8881 gagtatatcg ttaatgcaaa aaaatatttt aataatctta ctgttcattc attaaacagt
     8941 aactatcgct caaaaaaaga gattgttaca ttgtccaatc gattgatagc aaaaaataaa
     9001 ttcaggtcac gacgctggat taaagcggtg aggggaaacg gaggattaat actatttcat
     9061 aaagcttcat cgtttgctga ggaagtaact ataacagcat ctatcctgga aaaatatgat
     9121 agcacacatc tcataggggt gctataccgg aataactggc agggcactta tttgcaatct
     9181 cagatacaaa gttatggcaa tgtacaattt atgacaatac attcagctaa ggggctggaa
     9241 tttgatgtag ttgtgctttg tggtgttaag gacagattgt tgcctgaccc ctatactgat
     9301 attgaggaag aacgacgatt gatgtatgtt gcgttaacaa gggcaaaaaa ttgcttgcat
     9361 atactatacc attgtaatta tggtaatcaa aagccacaat ttgttatgga atgtgaaaga
     9421 tgtttttgat ttttgttgta acataccatc tggtcccttt ccaaacatag agatagtgct
     9481 gtactaaaca actggacttt aagcaaccgt atggattata ttcttgaggt aaacaatctt
     9541 tataaaggtt ttcctggtgt taaggctgtg gatggagtaa gcttttcaat accctatggt
     9601 atttgttttg gtttacttgg tcctaatggt gcggggaaaa caacaacaat tgaagttata
     9661 gaagggattc aaaaacctga tgaagggata atattatata aaggtaaaaa tcgtgatcac
     9721 cgatttaagc aggaggtcgg gatacaactc caggcaacag aattacagca atatcttact
     9781 gtaaaagaga tacttttgtc ttttagaaat ttatatgcga aacgtgctga cctgaatgat
     9841 ttgatagcaa tgtgctatct tgatgagata ttaaaccgag ataacaggaa aatttcaggt
     9901 ggtcaaaagc agcgtttgct tctggcaata gctctggcaa atgatcctga attattattt
     9961 ctggatgagc caacaacagg attggacccg caggcacgga gacatgtgtg ggatatagta
    10021 caaactgtaa aaatgaaagg taagacgata atattaacaa ctcattatat ggaagaggct
    10081 caaaatttat gtgatgttgt tgcaattatg gatcagggta aaattattgc tatggattca
    10141 ccaatcaatc tgctaaacca atattgcagt ggggttacga taacattacc ttcggtaata
    10201 gatgttgata tgttaaatag cattgaatgc aattggtaca tggttaataa cacaatcgaa
    10261 attcaaactg agaaacctca tgattgctta cgcgttctca tcgataaaaa tattgatatt
    10321 accggcatgg tggtgcgctc acaaaattta gaagatttat tcttaaaact tacgggaaca
    10381 caattaaggg cgtaactttt tatgttatat agaatctgga caatatttgt agcccgaaca
    10441 aaagagtttt acagggatag gtctgcactg ggatggaatg ttatttttcc tctattaata
    10501 attgttggat tcagtgttgt atttcgaagc gattcttcgc ccattttgaa ggttggtatt
    10561 gttgcattag cagaaaaacc ggcggaaaat ggtatatcca atatacagtt taatgaattt
    10621 ttaaagatta aatatataaa ttttatccaa tttaattcta aggaaaaagc aatcggaagt
    10681 cttcaaaaat ttaagattga catggtgatt gatagcaaca gcaaaacata ctggattaat
    10741 gaatcatcgc ctaatggata tctggcagag cagttgctca aaggagcaca aaacaagagt
    10801 tctgttttta aaaagcagtc attttacaat aaaggtgtac aatatgtaga atggcttttt
    10861 ccgggtattc tcgggatgaa tgccatgttt aattcactat atggtgttgg atatgttctt
    10921 gtgatgtatc gtaaaaacgg tatattaaaa cgttttagtg tagctccagt gcggccattt
    10981 gaatttttaa gtgctcagat actatcacga atgtttgtgg ttttagctac tacagttgtc
    11041 gtgtatgttg gaacagtatt gctctatgga tttaagtgca aaggatcatt gtttacgtta
    11101 tttattctct tcgcgttagg tagtttttgt atgatctcat tgggattact tattgcatca
    11161 cggagcgata gccaggaatt tgctgatggg ataatcaata ttataacatg gcctatgatg
    11221 tttttatctg aagtatggtt ttcactggag gggagtaggc cctgggttca gaaagtttca
    11281 cagtttttac cgttaacgca tatcgtagat ggtgcaagaa tgataatgaa tgaaggagca
    11341 acactatttg atttgcaact ccagatagta tatttagttg ctatctcatt attgttttta
    11401 ttccttggtt cattactttt tcgatggtaa agaaaccagt cctattttta gtaaagaata
    11461 cagaaaatta tttgaattga aatataattg acactataat aaaatgttaa ataatgtcat
    11521 aagcaattga tgatgtaaca catggtggat gtagctcaat ggcagagccc tggactgtgg
    11581 atccagttgt tgcgggttcg agccccgtca tccacccata atataaacgc gcccgtagct
    11641 cagctggata gagcgtcgga cttcgaatcc gcaggccgca ggttcaactc ctgccgggcg
    11701 cgcagtgtaa tatattcaaa caatgaacag tactgttaaa gattttgata tcttcaatta
    11761 tctgcctaaa gaagaagatt cagtcatcaa taacccgtat cttcaggaaa tattaacata
    11821 tcgtaataat caatttgatg aatatcagta ctggaatgta cgatacgatt taattaaaaa
    11881 atatgctttt gctgtaccta ccattgatac cataaaaata attcaggagc tatcgcccat
    11941 catagaaatt ggtgccggta acggctattg ggcttatatc cttaatcagg tgaaggttga
    12001 tataatagca actgataagt accctccgga cgaaacagct ttgccatttt caaattattc
    12061 cagcaatcaa tggttccata atcaatggta tgatgttata aaagccgaag caatagaagc
    12121 aggtaatcat cctgaaaggg cgttgatgct atgctggcct gaacctcaaa gcatgatggc
    12181 gtatgaatcg ctatatgctt ataaaaatgc cggaggccat actataattt ttattggtga
    12241 tatgatagca tgcgctgaga gcaactttta tgatgcttta gcacaatgta aactagaggt
    12301 taaacaacag atatggggat ataaatactt taatgagata attgcaatat acaaaatata
    12361 ctaagtatta agatatatgc ggtgttattt atgttatttt gatacaataa acactattaa
    12421 ttattgaaat attaattgtg agagatagta tgatagaagc gcataaaaaa atattaattg
    12481 tatcaatgcc tatggtagat agtgatttga aattatatct ggcgccatca cgatgtattg
    12541 acagtgatga tccattagta gttgattttg cacattctgc aattggttct gaagcagatg
    12601 cggtaaaaaa atcagtgaaa cttttttatg cagtacggga taatatatat tatgatccct
    12661 attccccatt ttataaacct gaacattatc atggaaggtt tgttttgcaa agaggtagag
    12721 gattttgtgt ttccaaagct tctttattat gtgcgcttgc aagagcttgt aatatcccga
    12781 gccgcgtagg ttttgcaaca gtaaaaaatc atatagctac aaaacaatta cttgaaaccc
    12841 tgggaacaaa tgtttttgct tatcatggct ttaccgaatt ttatttgaat aaaaaatggg
    12901 ttaaagctac ccctgccttt aataaagagt tatgtatatt gcataaagta tcaccaattg
    12961 aatttaatgg tgttgatgat gcaatcttcc cggaatataa ttcagaaaat aaactgtaca
    13021 tggaatatct tgactatcat ggaagttttt atgatgttcc tgtcgaaacc atcattacag
    13081 cgtggaaaga atgttatgga agcgaacgag ttgagttgtg gattaccctc tttgaacaaa
    13141 attttgctaa tagaaaattt gagggtgaag agatcgttaa atagtttatg cagatagtgc
    13201 ctattattga tattttgaaa tttattgatt gtgattttat ttgtgtagat agtttattgg
    13261 aaattattca ttccaacaat gactctttta taaataaaaa tattgctgat acattcccct
    13321 attcattaat aattgatgat gtaaaaagat attgcctgga actatcaaca aatgccggtg
    13381 ttaaaacagt agtaaaattt caaaatgaaa attgtttata tcaattttca ttgtttgata
    13441 gtaaatatgg tgataaaacc attatggtta ttattgcaaa acccaaaaaa caaataccag
    13501 aagtaataca ggacatatgc cataatttat ttaaagtaaa gattgctgat gaccttcaac
    13561 attttgttga tgtttttgaa tatattttaa attcaattat tcaagtaacc ggaatggatt
    13621 gtggaggcat ctatattatt gatcagctta ccatgaaact taaacttact tttgccagag
    13681 gtttgactaa ctcatatatt aaatctgcat ccatttataa caacgaaagc tggaactatc
    13741 agttagtaat gaatggcacg ccgcaattta ttgattatac aaacatggaa ctttgtgaca
    13801 atgattttag acgattggaa ggaataaaag ctgttgcaat agtacccata aaatttagaa
    13861 attctataat tggtgttata aatgttggat cccattctac aaatacaatt tctcaaaata
    13921 tatatccgct gttagaagtt tttggtgaac agcttggtac cattatggat aactatatgg
    13981 ttaaaagtgt attggctcaa tcaaaatata aaaaggaaat aattgatacc atacatgatg
    14041 tggtgtttca actggataaa gatggtatag tacaatatat gagcgggaat ataagtacta
    14101 tcacaggatt tacacctgat gaaatacttg gtaaatattt tataaacttt atctatcctg
    14161 aagatgtggc tatgtttatc gaaagttgcg aactggctaa aaggaggata tttgaacccc
    14221 ttgaatatcg attaattact aaagagggaa gctatcgata tatcttaacg atatctgaat
    14281 cacattatga tgaaaaggga aactatattg gattaacagg agttgttact gatattcatg
    14341 ataagaaatt agctgaaata aagctgaaag aaagtgatga acgttttaaa acattagctt
    14401 cgtcggtagc agctggtatt tttgttatca atgacttcag ggtgtattat gttaatcctg
    14461 cagttattca aattctggaa tacccagaag aggaaattct gggaaaatta ttttgggagt
    14521 ttatatcaga agaagatagg ggatggataa ttgaacgggc aaaagctcga ttagaaggaa
    14581 aatctgttcc aacccgatat gaattaagac ttgttacaag aaataataca attaaatggg
    14641 tcgatttttc cgctacgcag atcatgttaa acggtaaacc tgccacattg ggctcagtat
    14701 ttgaaattac agaaaaaaaa cgtattgaat cggaattaaa acaatcagag gaaaaatttt
    14761 ataaggcatt ccattccagt cctatcccaa tgactatcaa tacctataac gatggaatgt
    14821 acagggaagt taacgaggct gcattaacca catttggttt ttcacttcag gaagcattag
    14881 ggaaatccat ctatgatctg gatgtgtttg cagataaaaa tgagttaaaa caatatttac
    14941 aggaattacg aactaaccgt gaagtcaaag atatggatat tcttttccag acaaaaaatg
    15001 ggaagctgaa gcagtgtagt ataaattcgg atatttttat ttcttcaaag gaggaatttg
    15061 ttgtcagctt gattgttgat atcacacaga taaagcaaaa tgctcaaatt attttagatc
    15121 aatttaatga gttgcaaaag ataaatgctg atttagctaa tgtgcaggca aaaattacca
    15181 cccagaatat acaattatca gaagagaagg aacttttaaa tagaatatta gagtcaataa
    15241 cagatgctgt tattgtaacc gatagttatg gtaaaatagt tcttgttaat actattacct
    15301 gtagcatatt aggaaaacca gctaatttga tcctgaatac tatggcaagt gatttactca
    15361 ccatagagtg caaacaatct gtttcactaa attttaagga aatatatgat actttgaaaa
    15421 acaatagtat tcatcaggaa tgtatattac gggttaatga gaggaagtat gatattgaat
    15481 ttaatttatt gccattaaaa aatcaacaca atgatgtgaa tggcgctgtt attttattgc
    15541 gagatataac acagaagaaa attgttgagc aacaaatggc aaagacctct aaattagaat
    15601 caactggact tttagctgct ggaattgcac atgattttaa taatatatta acttctatag
    15661 ttggtaacct ttcaatcgta aaatataaaa ttaatgaaaa ttcagaacta tataatagta
    15721 tacgtggcgc cgaaactgct gcatttaaag caaaagaact tactcagcaa ttattgacat
    15781 tcgcaaaagg tggttccccc cttaaaaaac ttacttcatt aaaaaatatt cttgtagaaa
    15841 catctacatt cgtattacgg ggtacagcca tcgaattccg ctttataatt gccgatgatc
    15901 tatacaatgt tgaggttgat gaagcacaga tagggcaggt tatacaaaat atagtcatta
    15961 attcacgtga agctatgcaa ggatatggcg ttttaaaaat tgcggcacat aatgttatag
    16021 aaaatgatcc cgtacgagtt attataggta atgaattata tgtacaaata tcaattgaag
    16081 ataatggagt gggtatacct cgggatattt tagaaaaagt atttgatccc tattttacaa
    16141 caaaaccgca tggtacagga ttagggcttg cggtagtcta ttctgttata aagcagcatg
    16201 gtggttatat ttttgttgat tctgaagctg gaaaaggttc gcgttttgaa atatatctta
    16261 aagccagtaa aaataaagct acattaacag agattcctta ttgtgatagt ataacagtta
    16321 caggctgcgg aaaagtatta attttagagg atgacagcaa tatacagttt attttaaaaa
    16381 caatgctgga agaattggga tttgaaattg atattacaat tgatggagaa gaagcattct
    16441 ataaatatat gaatgcaaag tataataaca ataaatatgc ttttgcaatt atggatttaa
    16501 ctattcccgg taaacaaggt ggcaaagata caataagaca gatacgggct atagataaag
    16561 atttcaaagt tatagtatca agtggatatt caaatgatcc agtcatgtca aattactctg
    16621 aatatggatt taatgcaata ttaccaaagc catatcgatt ggaggatctt aaaaaggtta
    16681 ttgccacaat aatggcttaa ataatatttt tttgtttttt taaataatcc ggctattttt
    16741 tattataata gtaattgtaa agaatattta ttgtagtata ataaaaaaaa cttgacaccg
    16801 tctggtataa taaactattg ctttaatgca atgagtaaaa aaatgttctt tggtaataaa
    16861 gtcaggtaaa tggttatacg agaaagggtt gctagctcaa ctggtagagc acctgactct
    16921 taatcagatg gttccgagtt cgagtctcgg gcaacccaat ttccgctacg ctgtgaaaat
    16981 ttagcttgaa aaattatatt gtttttctga tattgtttac tgatatgcga gcgtggcgga
    17041 actggtagac gcgctagact taggatctag taccttatgg tgtgggggtt caagtccctt
    17101 cgctcgcaat aagcgggaat aactcagcgg tagagtgcaa ccttgccaag gttgaggtcg
    17161 cgggttcaaa tcccgtttcc cgctcatata caataataat cccgataata atatcgggat
    17221 tattattata aaaggttgta atttaaccca tataataatg atttacaact cataagtctc
    17281 atggtaattg aatgctggtc atattataat tttcacaagc atagggaata ttatttcttg
    17341 atatagattc gtagacagcg ttttcctttt ctattgttct ttccgtgtcc gcggtgcctt
    17401 ttttatattc cactatccag cttgcctgac tttaaaagtt tttttccaaa aaattattga
    17461 cacttatgct atattagata tgaaggtcaa cgtatcatgc tggcgtagct caatcggtag
    17521 agcagctgat ttgtaatcag cgggttgggg gttcaattcc tctcgccagc tttgaaggtg
    17581 aggttcccga gcggccaaag ggggcagact gtaaatctgt tggcgaagcc ttcggtggtt
    17641 cgaatccacc cctcaccaat attaaaatta aaaaattagt aagtaaaaaa ctaaataact
    17701 tctttttggt taataatcaa tctattattt aatttaaatg aatatgtatc gtataagaaa
    17761 taccgtgttt tatgttgaac atttacttta tggtattatt taatttttcc agcatttcat
    17821 taatttgggc atcagttaat ccatgtgctc tgcttccctg ttcgatagtt tcataaattc
    17881 ctatatggca accaatacaa tgcaaaccat atgatgcaag aatagaagct gccacggggt
    17941 atttttgaac gagttcacca aatgtcatat cttttgtaat ggatgttttc attgttataa
    18001 cctcatatat acttactgct ataattattg taagctaatt atagcaatta tataatattt
    18061 tttcatatta ttaatgtaat aatctttact atatgataga ttaaatttaa ggtttaaaca
    18121 tacatatatt tatataagcg tttataataa gttatttttt tattgaatat aatttcatgg
    18181 atatctatta tggcattcat gtagtctaga aatacgatta agaaaacatg aaaatctcaa
    18241 catcattttc tgagtagcta tattaaaaat agtatgaaac aacaaataaa gaatagaaaa
    18301 actattaaaa ccaggataat catttattgc attatatgtt taataatttt atataaacat
    18361 atcaatgtat tggctgatag ttatgcaaat gccagtttgt ctgattgttt gcagattgca
    18421 ttgcagaata acattagtat aaaaatcgca tttgaggaag taaacggtgc tttatctaat
    18481 gtaaagatta caaaagggag caacaatatc caggtaaatg gggaattgcg gacaatagaa
    18541 attttaaagt ctgatgctgc taacagctca ttcaatgtac ctggcaagga tacattaata
    18601 gggttattta ttggtgcttc agcaacttat aaattatacg atgctaacaa aagtaattat
    18661 gaacagctta aattcttaga atataattta gcaatattat ctgctttaaa gataaaaaat
    18721 aatgtcatgc gagatgtaaa gataacgtat tatcaactgt tgcttgcaaa aaagaaaagt
    18781 gaattattgg aaaaattagt aaaaagtttt gaaataaaat atctgaatgc tgagcaatta
    18841 tttacaagtg gtcaacgtac aatggttgat gtttcaaaag ctcaggttga tctgacaaat
    18901 gccagattaa aatatgag
//
LOCUS       DAOSOX010000027        18763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_2958_length_18763_cov_7.640742, whole genome shotgun sequence.
ACCESSION   DAOSOX010000027 DAOSOX010000000
VERSION     DAOSOX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18763
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2958_length_18763_cov_7.640742"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLX22_03520"
     CDS             <1..494
                     /locus_tag="PLX22_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT18998.1"
                     /translation="GYALISNMTVYENIALPLRYHTDMPDSEIHELVDSLINYMHLSH
                     CRDVRPVMLTQSEMLRTAYARAIALNPDMLLVEHPLEGQCLWNAQIFLSALQNRAFTE
                     NKTVIVVTYQPSEYIDIATRLIMLHDGRVVFDGSKDEFEKTDNDYIKQYVALSPYGPM
                     TLR"
     gene            505..1185
                     /locus_tag="PLX22_03525"
     CDS             505..1185
                     /locus_tag="PLX22_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOT18999.1"
                     /translation="MKLEKNEIRVAIFILLPLVILLVVIVLKLGYSFATSTIDVYMKV
                     DNISSIKNGTQVKIKGYTVGRVVDIIPVYKPTLHFLALMRIKKEIDLYEDCSAIIMNQ
                     NIIGDTIIEIRNPVRKVRLLQSGAVVEGVEYVSLDTIVQDVHQLLATLTSTVGVIQDI
                     SMESRGNIRSLVANLSKSVETISSVLQDSQKDMLSIMASFRETAKTMNEISIELKKHP
                     IKFLFKSE"
     gene            1194..2066
                     /locus_tag="PLX22_03530"
     CDS             1194..2066
                     /locus_tag="PLX22_03530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT19000.1"
                     /translation="MPFFSVVIPVYNRAHTITKAVESVLGQSLADYECIVVDDGSTDT
                     TCKELSPYRKKITYIPIPHGGVSTARNAGIAASRGQWIAFLDSDDVWQSKKLEAHFNY
                     IQLHPDMMIHQTDESWIRRGKRVNPMKKHQKKEGYIFNESLHLCLISPSAVVVHRSLF
                     DTVGLFDEKLPVCEDYDLWLRVTWHYRAGFIPDKLIVKYGGHADQLSSQYWGMDRFRV
                     YAICKLLACYGSKLQAPQYTMAVEVALSKCRILLHGANKHSNVALAQKVTRVIEWLTA
                     DRQSMQAFPDLVEG"
     gene            complement(2014..>2262)
                     /locus_tag="PLX22_03535"
     CDS             complement(2014..>2262)
                     /locus_tag="PLX22_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19001.1"
                     /translation="DVTEYPVVKKRYGQSQINNTDDIFTITYPAATGGSVWYIWDWNK
                     GKLYSREFFEEPFLYSIISNNLILLQGQEMLACFAYQQ"
     assembly_gap    2263..2362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2363..2838)
                     /locus_tag="PLX22_03540"
     CDS             complement(<2363..2838)
                     /locus_tag="PLX22_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19002.1"
                     /translation="MTGLLAGMLLAFIPVFDTHTGFVLQPLWHGALHKIQTIEPSSTK
                     IDKLSFAITTDSGYYHLDANGALKRFIAGGDNFIKPTIDGNFYISYKKVGNDITMYNA
                     TNEIFWKIASAEYPYSAPDGSIIVLLTADGSSARIADFNGNIYANTQLNGQFCTAVA"
     gene            complement(2859..3860)
                     /locus_tag="PLX22_03545"
     CDS             complement(2859..3860)
                     /locus_tag="PLX22_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOT19003.1"
                     /translation="MPKKMKSQDTAQPKASPPAKRTEAKEKHVYHEKTAKHSKQKKQF
                     QNDSDIIKTNFDDFTDSSISWYLDQINKIPLLSREDEDTLARRARAGDHKAKEELIKA
                     NLRFVVSIAKKYQTSGISLLDLINEGNMGLIKAAERFDPDRGYHFISYAVWWIRQAIL
                     LAISQKTSLIRLPLNRTADIQKIDRVQKTLESKLGREPSPAEIAEELDMDNDEINHLR
                     NITQDFVSLDATIGNSEDNTVLGLVEDLNVESPDKKIFDESLQEALNEVLNTLTPSER
                     EILKLRYGLDGDEPLSLQQIGERFKLSKERIRQIEKKAIRKLRLPSRSQKLKSFLTD"
     assembly_gap    4381..4480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4481..4915)
                     /locus_tag="PLX22_03550"
     CDS             complement(<4481..4915)
                     /locus_tag="PLX22_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19004.1"
                     /translation="MIAILSNKKLGIVLFCMVFLLCCASYMPVEKIYEDSKIVKIILR
                     DKKTITGWYIVNYDATNRISSINKYIPNKNTPIVTIQLQYRGKLLSSYTISSSLPESD
                     TILYQSTHILNYLPTNDLKRVDISFNKPASIKQAKTTFVRIQY"
     gene            5105..6388
                     /locus_tag="PLX22_03555"
     CDS             5105..6388
                     /locus_tag="PLX22_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19005.1"
                     /translation="MIQYILRNAIMIFEEIFKNIWKALLSSFGIILLIAFLVLYLSLR
                     ESVTKFVDKRLFGSLDINEMIIEPKAKSGEPTLAYAINSISAEKVKAIRKMPTISSVN
                     AVTCINAATKIRIELFGKSYTPYVPICGVSRQFIKDKVADYKSFTFNPNKEVPVIAPQ
                     VALDMFNNFASINNIPLFTEETLKGLPVTLIIETPKAVEPKKRFKVPAYVHSFSSTFN
                     LTGIVVPDSFINSIAAQYAVETKSAKPILYIRLYAKVKDVKMLPQITKKIKSMGLAVT
                     SLDEIASKTNQAMSIIDGFSLLVGFFLLLLTVISIFNSYLIIIYNRSYSFSLRRILGV
                     SKLRIIMTFMVEAAFIGGVYGFIGYLAGAYSIQAAGARLSSLIPAFQGIGLEPVGMHI
                     GALMVLFSIAISSLSALIPAIFASNMNLFKALSKQ"
     assembly_gap    6574..6673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6674..6958
                     /locus_tag="PLX22_03560"
     CDS             <6674..6958
                     /locus_tag="PLX22_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin ABC transporter ATP-binding protein"
                     /protein_id="HOT19006.1"
                     /translation="RVAIARAIINEPKLILADEPTGNLDTATSHDILKLFAQLRDDKG
                     ISIIIATHDERIVKASNVQYHIEGGKISKNNIGKAGQKKKKALLLKTKKR"
     gene            7197..7970
                     /locus_tag="PLX22_03565"
     CDS             7197..7970
                     /locus_tag="PLX22_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19007.1"
                     /translation="MIVDKITTDSYLTDSMEEMKKAKIRSYFDLISILREHPDWLDEL
                     RKLILTEELINLPKKFDEFVKQYQNFIENEFKPLKQTVQKQGEDIAVLKEDVAVLKED
                     VAVLKEDVAVLKQDVGVLKSDVGDLKGDSFERKVRERAPSYFGKIIRKCKLISHIELA
                     NILDDALDTGIIGDDERDDALLCDIVVTGALRSDKERKVLIVAEVSVVVDKQDVERAA
                     SRTKVIEKCMGLPGIPVVIGKEHTEGALKRADELQVIVI"
     gene            8324..9235
                     /gene="murB"
                     /locus_tag="PLX22_03570"
     CDS             8324..9235
                     /gene="murB"
                     /locus_tag="PLX22_03570"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT19008.1"
                     /translation="MCSNDIVQELSHHGRVYTNIPMSKLTTFKVGGPADILIEPYHND
                     TIPSIVTIAKQVEMPVTVIGGGSNIVVGDKGIRGLVLRIGSSDDTPVAIIIQGDIVTV
                     DARSTKKFFVNQALEHGLEGMEFMAGIPGCIGGGIVMNAGTTMGTFSDIISHITFYDV
                     DGTLQTIGNETLFSYRKSMIPDGAIIISAECKLNHARNVEDIKKTIKEILIERKNKHP
                     LDYPSAGSVFKNPDGHSSWKLINDAGLRGYGIGGAKVSQLHTNFIINTGKATALDIKH
                     LVEFIQETIFKLYAIHLEPEIRFIGEF"
     gene            9241..10047
                     /locus_tag="PLX22_03575"
     CDS             9241..10047
                     /locus_tag="PLX22_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT19009.1"
                     /translation="MEFTAPVNNTLFCALDCETTGLNPIIDRIVSIGLIHFTVDRIID
                     SFYTLVNPLKPIPPHVTLLHGITAPMVEEAPTIAELFATIEQFTAKAYCVIHNPRFDL
                     SFIDSAFHQYNKTTPDIKAFDTVHLARKTFKQMPSYNLGKLCSHFGITINAHHALSDA
                     EGSMEIFKRIIEKLNAANWCIRDLERYHGGIISADSLAMRSTRNPIDSRIPLGYRITI
                     IYKDASGNMLERSILPQRYFIIGKNNYIQAYCYLRNEIRYFNTARIVAVH"
     gene            complement(10127..11722)
                     /locus_tag="PLX22_03580"
     CDS             complement(10127..11722)
                     /locus_tag="PLX22_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19010.1"
                     /translation="MDNKWRFKLIVIIVFFPACVFALPKTVTLKGLVIFNQEYVIRAA
                     NIDTIPASRFTFEAIAQKITKFYNDIGYTLATVQLEKETPTSIILYVDEGRLNRIIFK
                     RLNTLDTLRIQYDFNLPHRIYHKPTVEMQLTHLKKKYAIKDIVVTIQPSRDYSNSLFQ
                     IDRLREPFNTNKLPMIPVIPITPDPRYDLILTVIPYTAEEIGGIRWGFDTSLRRGLIP
                     YIQYTHNDVFQKHDAFKVGFRTGFMYGINGNFSSPPSNTFNEIKSMYNFKPYFRDLFI
                     PQISSKIYHSKTGRKDLGLDQYNYIMMSGLIEPGFIPIERLKVYPGYGVEKVFIFKVK
                     EDPDSDEVQPDISKKTQVWQFIGIEANLDLIPFSLKQTTKRKLRLTLRRYSSDTHFYM
                     VTIDALFQFEFANYDLFLLQMDYRYTNNNVPFYYEEPVSSKTFKGFMTKDYYSTHIAR
                     TSGEYQVSVYRDFVYMGVYNDITLFKGKHILSGNQFGIAYGLSFHYIFFEQFEFALWW
                     GKDYLFSNQESQFNFKFSLSKKW"
     gene            complement(11712..12002)
                     /locus_tag="PLX22_03585"
     CDS             complement(11712..12002)
                     /locus_tag="PLX22_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT19011.1"
                     /translation="MLFSEEQVGFTSELLKSIAHPIRLKVLCFLMDGEKTVGEIEKEF
                     GSTISNISQHLTILRKMHIIHRRKEANFMYYSIKDERILKLISTLKNLYCGQ"
     gene            complement(12359..12988)
                     /locus_tag="PLX22_03590"
     CDS             complement(12359..12988)
                     /locus_tag="PLX22_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19012.1"
                     /translation="MELHNPEATQQALAILRSGDPFQWYVITLLALVVYVYFTEIQNK
                     NWQGIAAGLSLYMVHWFVEIINALIQHFSGHALWTVPTGTAFLLLIGVGVELSFMFSI
                     AGLIFSKVLPKDPRAKILGINNRLFIAIANAAFFSIFEIFLVKTPCFVWVYPWWGALP
                     VFITVYIPFWVVSLYCYDWQLKVQKMVIGSMFVVNAVMLVVFAGFLHWI"
     gene            complement(13135..14118)
                     /locus_tag="PLX22_03595"
     CDS             complement(13135..14118)
                     /locus_tag="PLX22_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT19013.1"
                     /translation="MPDDVYKELSKRLMFENSTLLPQIWKCIANELEAQIIAFLPANA
                     TTIAENFHIPMDKATQILEELFKRGAVFKKAKPDAVEYRPPKHIVQFHDASLLWKNAP
                     SDFKNLWVQFMDTEYPPLLEVVTRVGIPSFMRVIPINATLKPRSEVLIYEDAYSIIEQ
                     ANEIAVVDCVCKLSHGNCSKPLNVCIQINNGAQYSLERGTGRKITKEEARDILKLSEK
                     EGLVHMTENKGFGNAICNCCSCCCEMLRFAGNKATMGVVAKSRFLAQVDENNCNACDV
                     CTTVCPVHAISIDDIATIDTELCIGCGLCASHCPAQAIELVQTRPREHIPL"
     gene            14554..15252
                     /locus_tag="PLX22_03600"
     CDS             14554..15252
                     /locus_tag="PLX22_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT19014.1"
                     /translation="MVTFEDAKTVATEIVNLVNPLSIVVFGSVAREGRGQDLDLFIVV
                     DDGVSSKATVAILQKKLKHYYQKFAIDEFVVNQSVLQEYYNKGSYFVKLIIKEGRSLY
                     MKEAIVQWMKQAEEELNVSLYLLQGKFYKGSCYHAQQAIEKAIKALLLNKGWDLEKIH
                     NVNRLMALCNDYTIHISLSDDDIVFIDSIYKGRYPADIGLLPLGEPDEHDAARATTIA
                     TQIVNEMNEYIKQK"
     gene            15252..16100
                     /locus_tag="PLX22_03605"
     CDS             15252..16100
                     /locus_tag="PLX22_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19015.1"
                     /translation="MKHAIVILLLTVSISVQAQMPDWAMMKDREGNTYYFDKSGKIYT
                     DENIEPAYKAVANDELEYYTSKVKTLWKAGYRKQALTIAKSIMALQETSQHVINGKKD
                     IAAFLRENQMKHGNRFVDISREAWLYLTREGNRMYLFNDKVPYIVSIAGNAEVISAKS
                     LDSLQYSQTVLRTGIRFTQTKGFDAIMTIAAESFAGTIESITVLQQHWKNVALDTFQR
                     TLIKSNDSMALYSFITGDALQLVGLELLIIKENRGYMVRIFTTQERFNQYKGEISAII
                     DGFSTP"
     gene            complement(16090..16563)
                     /locus_tag="PLX22_03610"
     CDS             complement(16090..16563)
                     /locus_tag="PLX22_03610"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT19016.1"
                     /translation="MNFTIRKANNNDINNILNILSPYVKQGILLPRNTDDIANNLSTF
                     SVAVTPKKLVGVISFYKYNNKLYEIRSLAVNGRYHHHGIGKQLVEYAINDIVQQSKEA
                     VIFCLTYNPAFFKKTGFTEVEKNTLPHKIWKDCQYCKHRDNCTESAMIYKAHIKE"
     gene            complement(16570..17118)
                     /locus_tag="PLX22_03615"
     CDS             complement(16570..17118)
                     /locus_tag="PLX22_03615"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT19017.1"
                     /translation="MQNFDFYQNNKSHYNDFVTQDLQHYTIAQNTIPHTHSQRNAHRM
                     LAAIIALIIISFTAGIVVGIKFATGNTRTIIDPQTKIALSNAAQKAKTIMAPQKKPQF
                     TKTEYPFTIKIGNTFNKNEATQLAALLSKTGQRIIIAKNKEHYHIFAGPYKTIDDAKK
                     SLKIIMGQQENGNFKNAIIIKR"
     gene            complement(17193..17930)
                     /locus_tag="PLX22_03620"
     CDS             complement(17193..17930)
                     /locus_tag="PLX22_03620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT19018.1"
                     /translation="MIQQRIRINKFLSLCGLGSRRKTEDLITGGLITVNGTVMRDLSY
                     QVDSAADIVMYKKQVLTPVQSFHYLIVNKPKGFITSANDERSRSTVFDLIPQRLKELG
                     IFPVGRLDKDTEGLLLFTNDGDLAYRLTHPRFKIPKTYEVLLDKPLRDDHKYSIEHGI
                     ELYGSITLPAIIKPLNDEWTAVTITITEGKKRQVRLMFTMFGYEIVSLTRIQYGPIAL
                     GNLPEGKYRILTRQEIEQLKSLPKLIS"
     gene            complement(17902..18657)
                     /locus_tag="PLX22_03625"
     CDS             complement(17902..18657)
                     /locus_tag="PLX22_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT19019.1"
                     /translation="MKVRLIICVIAASFFLVQCSKSGEELKYTAKLSKAVIINDNTSV
                     RIDPYIFSSRVSTVDKGTIVTVTDVSKEKQWVGGQIDYWYKVMLPNTIRGWVFGSSLA
                     LQIDASEAAIERYVDTLWKKEADTVRREIAGKWWSVNKSGDFTEHALELYPDGSYKSY
                     RKGSNGIEGSYSLNFRTSEIVFLDSTSFGKNLYFAKRGNAYKLTDAINDPGIEFKKIA
                     EFKDSMSDIEPESKVPNSPEGATPDDTATDTNQ"
BASE COUNT         5732 a         3195 c         3621 g         5915 t
ORIGIN      
        1 gggggtatgc cttgataagc aatatgacgg tgtatgaaaa tattgccctg ccgcttcgct
       61 accatactga tatgccagat tctgaaattc atgaactggt tgattcgctc attaactata
      121 tgcacctgtc gcattgcaga gatgtacgcc ctgttatgct cactcagtct gaaatgttgc
      181 gtactgctta tgccagggct attgctctaa atcctgatat gttgctggtg gagcaccccc
      241 ttgaaggaca gtgtttatgg aatgcacaga tctttctttc ggcattgcaa aacagggctt
      301 ttactgaaaa taaaacagtg atagttgtta cctatcagcc ttcagagtat attgatattg
      361 ctacaaggct tatcatgctt catgatggaa gggttgtatt tgatggttca aaagatgagt
      421 ttgaaaaaac cgataatgac tatattaaac aatatgttgc cctttcacct tatggtccaa
      481 tgactttgcg gtaggagtaa atgtatgaaa ctggaaaaaa atgagatacg cgttgcaatt
      541 tttattttat taccgcttgt tattttactt gtggttattg tgctcaaact gggctattca
      601 tttgcaacct caacgattga tgtatacatg aaggttgata atatatcgtc cataaaaaat
      661 gggacacagg taaagataaa aggatataca gtggggaggg ttgttgatat tataccggta
      721 tacaaaccca ccttacattt tttagcgctt atgcggataa agaaagagat tgatctttat
      781 gaagattgct ctgctattat catgaatcaa aatattattg gtgacaccat cattgaaata
      841 cgcaatcccg taaggaaggt gagattattg caatcgggtg cagttgttga aggtgtggaa
      901 tatgtaagcc ttgatactat cgtacaggat gttcatcagc ttttagctac tcttaccagt
      961 accgttggag taatccagga tatttccatg gagagcagag gcaatatccg tagtttagtt
     1021 gcaaaccttt caaaaagtgt tgaaacaata agctcggtat tgcaggactc gcagaaggat
     1081 atgctgtcca ttatggcttc atttagagaa acggcaaaaa ctatgaatga gatttcaatt
     1141 gagcttaaaa agcaccctat taaattttta tttaagagcg agtaatactg tacatgccat
     1201 ttttttctgt tgttattcct gtatacaata gagcacatac tataacaaaa gctgtcgaat
     1261 ctgtactggg tcagagcctt gctgattatg aatgcatagt ggtggatgat ggctcaacgg
     1321 ataccacctg taaggagcta tcgccctata gaaaaaaaat aacctatatc cccatccctc
     1381 atggtggggt aagcacagca cgcaatgctg gcatagcagc ttcacgtggg caatggatag
     1441 cattccttga ttcagatgat gtgtggcaat caaaaaaact tgaggctcat ttcaactata
     1501 tacagcttca ccctgacatg atgatacatc aaactgatga gtcatggatc aggcgcggta
     1561 aacgtgttaa ccccatgaag aagcaccaga agaaagaagg ctatattttt aacgagtcat
     1621 tgcatttgtg tcttatcagc ccttctgctg ttgttgtaca cagaagttta tttgatactg
     1681 ttgggctgtt tgatgaaaag cttccggtat gtgaggatta tgatctgtgg ttgcgggtaa
     1741 catggcacta tcgtgcaggg tttattcctg ataagctgat agtaaaatat ggagggcatg
     1801 ccgatcagct atcatcacaa tactggggga tggacaggtt cagggtatat gctatctgta
     1861 aattgctggc atgctatggt agcaaactgc aagcgccaca gtacacgatg gcggtggagg
     1921 ttgctctttc aaaatgtcgt attttgcttc atggtgcaaa caagcattcc aatgttgccc
     1981 tggcacaaaa ggttacacgg gtaatagaat ggcttactgc tgataggcaa agcatgcaag
     2041 catttcctga ccttgtagaa ggataagatt atttgagata atactgtaga ggaatggttc
     2101 ctcaaaaaat tcgcgtgaat atagtttccc tttgttccaa tcccagatat accagacgct
     2161 gccgcctgtt gcagcagggt aggttatggt aaatatatca tcggtattat ttatctgtga
     2221 ttgaccatat cgctttttta ctactggata ttcagtaaca tcnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nngcaacagc ggtacagaac tgcccattta attgtgtatt
     2401 agcatagata ttcccgttga aatcggctat acgtgccgaa cttccgtcag cggtaagcag
     2461 tacaatgatt gaaccatccg gcgcactgta gggatactcc gctgacgcaa tcttccagaa
     2521 tatttcgttg gtggcattgt acatggttat gtcattgcca acctttttat agcttatgta
     2581 aaagttgcca tcgatggttg gtttgataaa attatcaccg ccggcgataa aacgtttcaa
     2641 cgcaccgttt gcatcaagat ggtagtaacc gctatcggtt gttattgcaa aggaaagttt
     2701 atcaattttt gtactggaag gttcaatcgt ttgaatcttg tgcaacgcac catgccacag
     2761 cggttgcagc acaaaacctg tgtgagtgtc aaaaacagga ataaatgcca ataacattcc
     2821 agcaagtaag cctgtcatta catatcgcat agcgtttatt aatccgttaa aaagcttttt
     2881 aatttttgac tgcgtgatgg aagacgcaat ttacgtattg ctttcttttc aatctgtcgt
     2941 atgcgctctt ttgatagctt aaagcgctca ccaatttgct gtaatgacaa tggttcatcg
     3001 ccatcaagtc catacctcag tttcagtatt tcacgctctg aaggagttaa ggtgttaagc
     3061 acctcattca aagcttcttg caatgattca tcaaaaatct ttttatcggg tgactctacg
     3121 tttaaatctt ctaccagccc taaaacagta ttatcttcag aatttcctat agttgcatca
     3181 agtgatacaa aatcctgggt aatgttacgc aggtgattga tctcatcatt atccatatcc
     3241 agctcttcag ctatttcagc agggcttggc tctcttccca gtttgctttc taatgttttt
     3301 tgaaccctgt caattttctg gatatccgcc gttcggttaa gaggcagccg tattaacgat
     3361 gttttttgtg atatagcaag cagtatagcc tgccgtatcc accatacagc atacgagata
     3421 aaatggtatc ctctgtcggg atcaaatcgc tcagctgcct taataagccc catattccct
     3481 tcattgataa ggtcaagtaa tgatattccg cttgtttgat atttttttgc gatagatact
     3541 acaaatcgta gatttgcttt gatcaattct tctttggctt tatggtctcc agcgcgggca
     3601 cgacgcgcta gcgtatcttc atcttcacga gaaagaagtg gaatcttatt tatttgatcc
     3661 agataccacg atatcgatga atcggtaaaa tcatcaaaat tggtttttat tatatcacta
     3721 tcattttgaa attgcttttt ttgcttgctg tgttttgctg ttttctcatg atatacatgc
     3781 ttttcctttg cttcggtcct tttagctggt ggtgaagctt tgggttgagc agtatcctgc
     3841 gatttcattt tttttggcat accatccctt acattgtgca aattaaatgg tttacttaat
     3901 gttagtacaa taataaaatt ggcagcagat ttttacaatc taaaaaaagg aattaatgtt
     3961 ccctgaaatg tgtaaaattt tttactataa ggcatactat aagacaaatt ccagaataaa
     4021 gagcaatgca aaatacaaca atgttgtgtc ataatgagta caaccggtaa agtgactgta
     4081 acacacaaaa caaaacagga tgcatgtatg aagcactatg cccatatata gcaccatgaa
     4141 caaaaagtca tccaaaaaaa tgatgtatgc atcaaaaaag tatctggtca gggcaatgac
     4201 acgaaaataa ggcagacatc attcaagtat tgggaaagga taaccatggt atggcttctc
     4261 cagaaataga attatttctc aatgtaattc cgggcttaac ccggaatcta tataattata
     4321 cgaggtcctg aatcagactt cagaatgact gtcgcaaaat atttttattc cctatggatg
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtattgtatt cgtacaaatg
     4501 ttgtttttgc ctgtttgatg cttgcaggct tgttgaatga aatatcaacc cgttttagat
     4561 catttgtggg tagatagttc aagatatgag tggattgata gaggattgta tcactttcgg
     4621 gtaacgacga tgagatagta taactgctta ataattttcc tcgatactgc agttgaattg
     4681 taacaattgg tgtattttta ttgggtatat atttgttgat gctgctaatc ctgttagttg
     4741 catcgtagtt aacaatatac caccctgtaa ttgttttttt gtctctaagt ataatcttta
     4801 caattttact gtcttcataa attttttcaa ctggcatata ggaagcacag cacagcaaaa
     4861 ataccatgca gaatagtact attcccaatt ttttatttga cagaattgct atcatgatcc
     4921 cattgcatta gtggtttaca atattttaag gattattggc aatgatgtat aaaaatgcaa
     4981 taattttttt attatttaag ctattaattt caatttatta atgacatgag aaataggtat
     5041 tgattataat actttattac cggtgattgt aatttatttt taattgaaaa ggattattga
     5101 tactatgata cagtatatac tgcgcaatgc tataatgata tttgaagaga tttttaaaaa
     5161 tatctggaaa gctttactat caagttttgg tattatattg ctgattgctt ttttggtgtt
     5221 atacctttca ttacgtgagt cagtaaccaa atttgttgat aaaaggcttt ttggctcatt
     5281 agatattaat gaaatgatta ttgagccaaa agctaaatca ggtgaaccaa cattagcgta
     5341 tgcaataaac agtatttctg ccgaaaaggt aaaagctata cgcaaaatgc ccactatatc
     5401 ttcggtgaat gcagtaacct gtattaatgc tgcaacaaag ataaggattg aactctttgg
     5461 taaatcatat acaccgtatg tgccaatttg tggagtaagc cggcaattca taaaagacaa
     5521 agttgctgat tataaatcat ttacatttaa tcccaataaa gaggtcccgg tcattgcacc
     5581 tcaagtggct cttgatatgt ttaacaactt tgcatctatt aacaacatcc ccctttttac
     5641 ggaagaaacg ttaaaaggct tgccagttac gctgataatt gaaacgccaa aagcggtgga
     5701 accaaaaaaa cgttttaagg taccagcata tgttcattca ttttcgagta ctttcaacct
     5761 taccgggatt gttgtgccgg atagttttat aaattcaatt gctgctcagt atgctgtgga
     5821 aacaaaaagt gcaaagccta ttttgtatat caggctttat gctaaggtta aagatgttaa
     5881 aatgcttcct cagattacaa aaaaaataaa atccatgggg cttgcggtga cctcacttga
     5941 tgagatagcc agtaaaacaa atcaggcaat gagtatcatt gatggctttt ccctgttagt
     6001 tggttttttc ctgttattgt tgacagtaat atcaatattt aattcgtacc tgataattat
     6061 ctataatcga agctatagct tttcattacg gagaatatta ggggtatcaa aattaaggat
     6121 tataatgaca tttatggttg aagctgcatt tattgggggg gtatatggtt ttataggtta
     6181 tctggcagga gcttattcaa ttcaagctgc aggtgcaagg ctttcatcgc ttatacctgc
     6241 atttcaaggt atagggcttg aacctgttgg tatgcatata ggtgcgttaa tggtgttgtt
     6301 ttctattgca atatcgtcac tgtcagcgct catacctgca atattcgctt ctaatatgaa
     6361 tttatttaaa gcattaagta aacagtgagg tggaatcgtg gcattattgt cggtaaactc
     6421 tattagtgtt aaatatgatg aaagaagagt ccttgataag gttggttttg aaattaacga
     6481 aagtactatt actgcgctta ctggtaaatc aggttcgggt aaaacaactt tgttaggagt
     6541 actatcagga cttttaaaac cagatgaagg cacnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnncgtgttg cgatagcccg agccatcatc aatgaaccca aattaatact
     6721 ggcagatgaa ccaaccggta atctggatac agcaacctca catgatatac tgaaactatt
     6781 tgcacaactg agggatgaca aaggcatatc tatcattatt gcaacacatg atgaacgtat
     6841 tgttaaagca agcaatgtac aatatcatat tgaaggtggc aaaatttcaa aaaacaatat
     6901 tggaaaagct ggtcaaaaaa agaaaaaggc actattgcta aaaacaaaaa aaagatagat
     6961 cttctcaatg aatggttcca attgttctaa acaccattcg ggatggcagt atagtatcag
     7021 aatagattga ttacaaagtc gtttcaggct ccctatggaa caataaaatg ctggaatgaa
     7081 tacggcatga taggagcgca tcattttatt ttaagaggag ggagagacaa taatcttttt
     7141 attgaaggga tgcagattcc ttgataaata tatttaatgt gtgccaggag gtaataatga
     7201 tagttgacaa aataacaact gatagctatc ttacagacag tatggaagag atgaaaaaag
     7261 ctaagatacg atcatacttt gatctgatat ctattttacg agagcatcct gattggctgg
     7321 atgagctacg taagcttatt ttgacagaag agcttattaa tttaccaaaa aaatttgatg
     7381 aatttgtcaa acagtatcaa aattttattg agaatgagtt taaaccgtta aaacaaactg
     7441 tccagaaaca gggtgaagat attgcagtat taaaagaaga tgttgcagta ttaaaagaag
     7501 atgttgcagt attaaaagaa gatgttgcag tattgaaaca agatgttggc gttttgaagt
     7561 cagatgttgg tgatttaaaa ggtgacagtt ttgaacgaaa ggttcgtgag cgtgcaccat
     7621 cgtactttgg aaaaataata cgaaaatgta agctaataag ccacatagag cttgcaaata
     7681 ttcttgatga tgcacttgat accggtatta taggcgatga tgaacgtgat gatgctctgc
     7741 tttgtgatat agtggttacc ggtgcattac gaagtgataa agaacggaaa gtactcatag
     7801 ttgctgaagt atcagttgtt gttgataaac aggatgttga aagagctgca tccagaacaa
     7861 aggttattga aaaatgtatg ggtttacctg gaatacctgt tgtgattggg aaggaacata
     7921 ctgaaggtgc cctaaaaagg gctgatgaat tacaggtaat agttatataa atactatatt
     7981 atgaagaagt taaaaaaaat ataattgatt ttcatgctat aatcttaata ctggtatatt
     8041 caataattat atagcaatgc tattatccaa actcatttca ggataaggat ttagcaacat
     8101 aattcaacat ggtcagtgaa cgtattgtgg caggcttagc accagcactg caatcatgat
     8161 ttgcgacagg cttaataact atagttgaaa aaatacaata accctgtgtg cttgagataa
     8221 tggtatgcga taatgcatat aatgaaggat tgcttgcatg aaattgaaaa aaattactat
     8281 ataattttaa ttttcaggag gtggtactaa cactgtggaa caaatgtgtt caaatgatat
     8341 agtacaagag ttgtcgcacc atggcagagt atatactaat ataccaatga gtaagttaac
     8401 tacatttaaa gtgggtggcc ccgctgatat tttgattgaa ccatatcata atgatacaat
     8461 accttctata gtaactatcg caaagcaggt agaaatgcct gtgactgtta taggtggtgg
     8521 ctcaaatata gtagttggtg ataaaggaat acgaggactg gttttacgaa ttggcagcag
     8581 tgatgacacc cctgtcgcta ttattattca gggggacatt gtaacagttg atgccaggtc
     8641 aaccaaaaag ttttttgtta atcaagcatt ggaacatggt cttgaaggga tggaatttat
     8701 ggcaggtatt ccgggttgta ttggtggtgg cattgttatg aatgctggca ccactatggg
     8761 tactttttct gatataattt cacatattac tttttatgat gtggatggaa cgcttcaaac
     8821 cattggcaat gaaacattat tttcatacag aaaatccatg attcctgatg gagctattat
     8881 tattagtgct gaatgtaagc ttaaccatgc cagaaatgtg gaggatatta aaaaaacaat
     8941 aaaagaaata cttatagaac gcaaaaataa acacccctta gattacccct ccgccggatc
     9001 tgtttttaaa aatcctgacg ggcattcatc atggaagctt atcaacgatg ctggattgcg
     9061 tggttacggc attggtggag ctaaagtatc acagttgcat actaatttta ttattaatac
     9121 tggtaaagct actgcacttg atataaagca tctggttgaa tttatccagg aaaccatatt
     9181 taaactgtat gctattcatt tagaacccga aatacgattt attggagaat tttaagctct
     9241 atggaattta cggcacctgt caataataca ttattttgcg cattggactg tgaaacaacc
     9301 ggtcttaatc ctattattga caggattgtg tctattggac ttattcattt tactgttgat
     9361 aggattattg attcttttta tacgctggtg aatcctctca agcctatccc accacacgtt
     9421 accttgcttc atggcattac tgctccaatg gtggaggagg ctccaacaat agctgaattg
     9481 tttgccacaa ttgaacagtt tactgctaaa gcatattgtg ttatccataa tcctcgtttt
     9541 gatctttcat ttattgattc tgcttttcat cagtataata aaacaacacc ggatataaag
     9601 gcttttgata ctgtccattt agcccgtaaa acttttaagc aaatgccaag ctacaattta
     9661 ggaaaacttt gctcacattt tggcattacc attaatgcac atcatgcttt aagtgatgct
     9721 gaaggatcta tggaaatatt taaaagaata attgaaaagc ttaacgctgc aaattggtgt
     9781 atccgtgatt tagagcgata tcatggtggc atcataagtg ctgattcgct tgccatgcgc
     9841 agcactcgta atcccattga tagtcgtata ccattgggtt atcgcattac aattatctat
     9901 aaagatgctt caggtaatat gcttgaacgc agtatattgc cacaacgata ttttataatt
     9961 ggaaaaaata attatatcca ggcatattgc tatctgcgga atgagatacg atattttaac
    10021 actgcacgta ttgtggctgt gcattagatt gtaagtaact atcgcccggc aatgtattaa
    10081 tttttttata gatagccgct gtaaatactc attgttaaat taaatattac catttcttcg
    10141 ataacgaaaa tttaaaatta aattgggact cttgatttga aaataaatag tctttacccc
    10201 accacagagc aaattcaaac tgttcaaaga agatgtagtg aaaacttaaa ccgtaggcaa
    10261 taccaaactg attgcctgat aaaatatgtt tacctttaaa cagtgttatg tcattgtata
    10321 cacccatata aacaaaatca cggtatactg aaacctgata ttcacccgat gttcgtgcaa
    10381 tatgtgttga gtaatagtcc ttagtcatga agcctttaaa tgtttttgaa gatacaggtt
    10441 cctcatagta aaagggcaca ttgttgttag tgtatcggta atccatctgt aaaagaaata
    10501 gatcgtagtt tgcaaattca aattgaaata gtgcatcgat agtaaccata taaaaatgag
    10561 tatcgctgct ataacgcctg agagttaatc ttaattttct tttagtggtt tgtttcaatg
    10621 aaaaggggat gaggtctaaa tttgcttcaa ttccaatgaa ttgccaaacc tgtgtttttt
    10681 tggaaatatc gggttgtact tcatcagaat ccgggtcttc ttttactttg aagataaaaa
    10741 ctttttctac accataaccg ggatatacct tcaaacgttc aatagggatg aagcctggct
    10801 caatgagccc gctcatcatg atatagttgt actggtcaag ccctaaatct ttacgccctg
    10861 tttttgagtg atatattttt gatgaaattt gggggataaa gagatccctg aaatatggct
    10921 taaaattata catggactta atttcattaa atgtattgct tggtggtgaa gaaaaattgc
    10981 cattgatgcc atacataaaa ccggttctaa accctacttt aaaggcatca tgtttttgaa
    11041 acacatcatt gtgagtatac tgtatgtatg ggataagccc acgccgcaaa cttgtatcaa
    11101 atccccagcg tatacctcca atttcttcag cggtataagg gataaccgtg agtatcaaat
    11161 catagcgcgg gtcaggagta atgggaataa caggtatcat tggcaacttg ttagtattaa
    11221 atggttctcg taacctgtca atctggaata gggaatttga ataatcccgt gaaggttgta
    11281 tggttactac aatgtcttta attgcatatt tcttttttag atgagtaagc tgcatttcaa
    11341 ccgttggctt atgataaata cgatggggaa ggttaaaatc atattggatg cgtaatgtat
    11401 ccagggtatt taaacgttta aaaatgatgc ggttcaggcg tccttcatca acatagagga
    11461 ttatagaagt aggggtttcc ttttctaact gtacagttgc taatgtatag ccaatatcat
    11521 tataaaattt cgtaatcttc tgggcaatgg cttcaaaagt aaatctgctg gcagggatgg
    11581 tatcgatatt ggcagcacga ataacatatt cctgattaaa tatcaccaga ccttttaagg
    11641 tgacagtttt tggcagagca aatacgcagg cagggaaaaa tacaataata actatcagtt
    11701 taaaacgcca tttattgtcc acagtataga ttttttaagg ttgagattag ctttaaaata
    11761 cgttcatctt taatagagta atacataaag tttgcttctt tacgcctatg aatgatgtgc
    11821 atctttctaa gaatggtaag atgctgggaa atattggaga tagttgatcc aaattctttt
    11881 tctatttcgc caacggtttt ttcgccgtcc attaaaaaac acagaacttt aagccgtatg
    11941 ggatgggcaa ttgattttaa aagctcactg gtaaatccaa cctgttcttc agaaaacagc
    12001 atgcataatt cctccacgca aatgatataa taccattaat gtcaaaatag taaacaagct
    12061 tttaagttct taattaaaca ataattttac tatatagtca caaagtagca atgaaagaat
    12121 aattgtcaat attttttata cttgtatact atttctctgg ctattgctaa tcaatttggt
    12181 ttgatagggt atagctatag tatgttcagc aggtaaaatc ctgatatact atcgccatcc
    12241 tttgctttat agccttttaa aaatggtgga gcgtatacca ttaacgatag gtatcctgac
    12301 tgtgcccgca cggcgaaaat aaaaacttca ataaaaaaca cctcaattat tgtttcaact
    12361 atatccagtg taggaaacca gcaaaaacaa caagcatgac agcattgaca acaaacatgc
    12421 tgccaatcac cattttctgc acctttagct gccagtcata gcaatataat gacactaccc
    12481 agaatggtat atatactgtt atgaaaacgg gcaacgctcc ccaccatgga tatacccata
    12541 caaagcacgg cgttttaaca agaaatatct caaagattga gaagaatgct gcattggcaa
    12601 tcgcaatgaa taaacgattg ttgataccca aaatttttgc tctgggatct tttggtaata
    12661 ccttgctgaa gataagccct gctattgaaa acataaagct cagttcaacg ccaacaccaa
    12721 taagaagtaa aaacgctgtg ccggtgggga ctgtccagag cgcatgcccg gaaaaatgct
    12781 gtatcagtgc attgatgatc tcaacaaacc agtgaaccat atacagcgaa agccctgcag
    12841 caatgccttg ccagttttta ttctgaattt cggtaaaata gacatatact accaatgcca
    12901 gcagggtgat gacataccat tgaaatggat cgccgcttcg taaaatagct aatgcttgct
    12961 gtgtagcctc cgggttgtgc agttccatag tttcacctca cttattaacg atgtatcacg
    13021 ttcttttagt ataacgaata tagtttatga agtattgtaa aaaaatgtaa aggcattttt
    13081 gcgatagtta ctgtgaaata tatacatgct aaaaagctgt gccccttgta tgtattacag
    13141 tggtatatgt tctcttggac gagtttgcac aagctcaatt gcctgtgctg gacaatgcga
    13201 tgcacataat ccgcaaccaa tacataattc agtatcaatg gtagcgatgt catctatgct
    13261 gatagcatgt accggacaaa ccgttgtgca aacgtcacat gcattgcagt tgttttcatc
    13321 aacctgtgcc aaaaatctgc tttttgcaac cacccccatt gttgccttgt tcccggcaaa
    13381 gcgcaacatc tcgcaacagc atgaacagca attgcagatt gcattgccaa agcctttgtt
    13441 ttcggtcatg tgcacaagcc cctctttttc agacagcttt aatatatcac gggcttcttc
    13501 tttagtaatt tttctaccgg tgcctcgttc taatgagtat tgagcaccat tgttaatctg
    13561 tatacagaca ttcaacggtt tgctgcaatt gccatgtgac aatttgcaaa cacaatcaac
    13621 aaccgctatc tcatttgcct gctcaataat gctatatgcg tcttcataga ttaaaacctc
    13681 actgcgcggt ttaagcgttg cattgatagg gataactcgc ataaacgaag gtatgccaac
    13741 tctggtgaca acctccagta gtggaggata ctcagtatcc ataaactgga cccacaaatt
    13801 tttaaagtca gatggagcat ttttccataa cagtgaggca tcatgaaatt gaacaatatg
    13861 tttaggaggg cggtattcaa ctgcatcagg ttttgctttt ttaaatacgg cgccgcgctt
    13921 aaaaagttcc tcaagaattt gtgtagcttt atccatgggt atatgaaagt tctcagcaat
    13981 ggtcgttgca ttggcaggta aaaatgctat tatctgcgct tctaattcat ttgctataca
    14041 tttccatatc tgtggcaata gtgtagaatt ttcaaacata agccgtttcg atagttcttt
    14101 atatacatcg tcaggcataa cataccctcc aaaaatagaa atagtaacct caccccttgc
    14161 cctttgtaag gcgaggagag gattgttccc gaaaatagaa ttacaccaca ccgtcattcc
    14221 ggacctgatc tggaatctct ttttcaccat gagatcctga atcggacttc agaacggcag
    14281 cagtgatagg tttacaaccc atttcatctg ctgtgcttca gtcaatgtga gatagattat
    14341 tagtataaaa ctataaatcc ggaattttaa gaaaacgttg ctccatattt caattaaggg
    14401 taaatgttat attacaaata ctttatgtca attattttta ttgtgggcga taggtattgg
    14461 caatccaccg ataaacatac ttgactaaat tcatgtaatc tgctatgcaa ttgttgaatc
    14521 tatcaaacca ttgtataaac gagtgaacgt ggtatggtaa cctttgagga tgcaaaaaca
    14581 gtagcaacag aaattgttaa tttagtaaac ccactgtcga tagtggtatt tggctctgtt
    14641 gcgcgtgaag gaagaggtca ggatttagat ttatttatag ttgttgatga tggcgtatca
    14701 tcaaaagcaa cagttgccat attgcaaaaa aaactaaagc actattatca aaaatttgca
    14761 attgatgaat ttgttgtaaa ccaatcagtc ctgcaagagt attataataa gggcagctat
    14821 tttgtaaagc tcattataaa agaaggaagg agcctgtata tgaaagaagc aattgtccag
    14881 tggatgaagc aggcagaaga agagttgaat gtttcattat atcttctaca gggtaagttt
    14941 tataaaggct catgttatca cgcacaacaa gcaattgaaa aagctattaa agcacttctt
    15001 ttgaataagg ggtgggatct agaaaagatt cacaatgtta accgtctcat ggctttatgc
    15061 aacgattata ccatacacat ttcgctttct gatgatgata ttgtttttat tgatagcatt
    15121 tataagggaa gatatcccgc tgatattggt ttgttgcctt taggagagcc tgacgaacat
    15181 gatgctgcac gagctactac tattgcaaca cagattgtca atgaaatgaa tgagtatata
    15241 aaacaaaaat aatgaaacat gctatcgtta ttttactgct taccgtgagt atctctgtac
    15301 aagcacagat gcccgattgg gctatgatga aagacagaga aggaaatacc tattattttg
    15361 acaaaagtgg gaaaatttat accgatgaaa atattgaacc ggcctataaa gctgttgcaa
    15421 acgatgaact ggaatactat acatcaaaag taaaaacact gtggaaagca ggttacagaa
    15481 agcaggcatt gacgatagct aaatcaatca tggcattgca ggaaacctca cagcatgtta
    15541 tcaatgggaa aaaagacatt gcagcatttt tgcgtgaaaa tcagatgaag catggcaatc
    15601 gttttgttga cattagcagg gaggcatggt tatatttaac aagggaaggt aaccgtatgt
    15661 atctatttaa tgataaagtg ccctatattg tatcgattgc agggaatgca gaggttattt
    15721 cagcgaaatc actggattca ttgcagtact cccaaacagt attgaggacg ggtatacgtt
    15781 ttactcaaac aaaagggttt gatgccatta tgacaatagc ggccgagagc tttgcaggta
    15841 ccatcgaatc cattactgta ttgcaacaac attggaaaaa tgttgcattg gatacatttc
    15901 aacgtacact gattaagagt aacgacagca tggctctgta ttcatttatt accggtgatg
    15961 cattgcaact ggtaggatta gagcttttaa taataaaaga aaacagaggg tatatggtca
    16021 ggatttttac tacacaagaa cggtttaacc aatataaagg tgaaatatca gcaatcattg
    16081 acggattttc tactccttaa tatgggcttt atatatcatt gctgattcgg tacagttatc
    16141 tctatgttta cagtattggc agtccttcca tatcttatga ggcaatgtgt ttttttcaac
    16201 ctctgtgaat cctgtttttt taaaaaaggc aggattatag gttaaacaaa aaataactgc
    16261 ttctttactt tgctgtacaa tatcatttat ggcgtactca actaactgtt ttccaattcc
    16321 atgatggtga tatcgtccat tgacagctaa tgaacgaatc tcataaagtt tgttgttata
    16381 tttataaaat gatataacgc ctacaagctt ttttggtgtt acggcaactg aaaaggtaga
    16441 aagattgttt gcaatatcat cagtatttcg tgggagcagt attccttgtt ttacatacgg
    16501 tgacaggata ttcaggatat tgttgatatc gttgttgttt gcttttcgta tggtgaagtt
    16561 catgtaagtt tagcgtttta ttataatggc atttttaaaa ttgccatttt cttgctgccc
    16621 cattataatt ttcaaggatt tttttgcatc atcaattgtt ttatatggac cagcaaaaat
    16681 atgatagtgt tctttatttt ttgctataat gatgcgctgt cctgttttac ttaagagagc
    16741 agctaattgt gtagcttcgt ttttattaaa tgtattgcct attttgattg taaatgggta
    16801 ctctgttttt gtaaattgag gttttttttg tggtgccatt attgtttttg ctttttgtgc
    16861 tgcgttgctc aaagctattt ttgtttgggg gtcaattatt gttctggtat ttcctgtagc
    16921 aaacttaatg ccaacaacaa tccctgctgt aaatgaaata atgattaatg caatgatagc
    16981 agccagcata cggtgtgcat ttctttgtga atgggtatgg ggtatcgtat tttgtgcaat
    17041 agtgtaatgt tgtaaatcct gtgttacaaa atcattataa tgacttttat tgttttggta
    17101 gaaatcaaaa ttttgcataa ttccaaaacc tttatgtgtt aagattcaat gacgtgtagt
    17161 attattttta tcgtcattca tgaaatagtg tattaagaaa ttaatttggg cagggatttc
    17221 aattgttcaa tttcctgacg ggttaaaatt ctgtattttc cttctggaag gttacccagt
    17281 gcaatgggtc cgtattgtat gcgggttaag gatacaattt cataaccaaa catggtaaac
    17341 ataaggcgta cctgacgctt tttcccttcg gtgatggtta tggtaaccgc agtccattca
    17401 tcattcagtg gtttaataat tgcaggtaat gttattgatc catacagctc tatgccatgt
    17461 tcaatagaat atttatgatc atcccgtagt ggtttgtcta atagtacttc gtaggtttta
    17521 ggtatcttga accgtggatg agttaatcta taggctaaat cgccatcatt ggtaaataaa
    17581 agcaatcctt cagtatcttt atccagcctt ccaacgggga aaatccccaa ttctttcaag
    17641 cgctgtggta tcaaatcaaa taccgttgaa cggctgcgtt catcgtttgc agaggtaata
    17701 aatcctttgg gtttgtttac aataagatag tgaaaacttt gtacaggagt aagcacctgt
    17761 tttttataca taacgatatc agcagcacta tctacctgat acgatagatc gcgcatgaca
    17821 gtaccgttaa ctgttataag cccacccgtt atcaaatcct ctgtttttct gcgggatccc
    17881 aatccacaaa gtgagaggaa tttattgatt cgtatccgtt gctgtatcat cgggtgtcgc
    17941 tccttccggt gagttgggaa ctttcgattc gggttcaata tcagacatgg aatctttgaa
    18001 ttcagcaatc tttttaaatt caatgccagg atcgtttatt gcatcggtaa gcttgtaggc
    18061 attacctcgc tttgcaaagt atagattttt tccaaaactg gtactgtcta aaaaaactat
    18121 ttcgctagtt ctgaaattaa gtgaatagct tccttcaatg ccattgctcc cttttctata
    18181 tgatttataa cttccatcgg gatacaactc aagtgcatgc tcggtgaaat caccgctttt
    18241 gttgacactc caccacttgc cagctatttc cctgcgaacg gtgtcggctt cttttttcca
    18301 taaggtgtca acatatcgtt ctatagctgc ttccgaagca tctatctgca aggctaacga
    18361 gctcccaaat acccagcccc gtatagtatt gggtaacatg accttatacc aataatctat
    18421 ctgcccacca acccattgtt tttcttttga aacatcggtt acagttacta ttgtcccctt
    18481 gtcaacggtg ctgacgcgtg aagaaaaaat ataggggtcg atacgtaccg atgtattgtc
    18541 attgatgatg acagcctttg atagttttgc tgtgtacttc aactcttcgc cagatttgct
    18601 gcattgtacc agaaaaaatg atgctgcaat aacgcagata atcaacctta ctttcattgg
    18661 gattcctctg attaaatact tttaccatat tttgggcgat agtttatggg taactatcgc
    18721 cactatgatg attacaaaac aacgataatg atataggtca tgg
//
LOCUS       DAOSOX010000028        17779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3206_length_17779_cov_8.453566, whole genome shotgun sequence.
ACCESSION   DAOSOX010000028 DAOSOX010000000
VERSION     DAOSOX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17779
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3206_length_17779_cov_8.453566"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /locus_tag="PLX22_03630"
     CDS             complement(<1..2352)
                     /locus_tag="PLX22_03630"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT19020.1"
                     /translation="MELHASYNPKIAESTWYTVWEQEGLFHGDENDPRDAYSIVIPPP
                     NVTGNLHMGHALNNTLQDILIRWQRMKGKATCWMPGMDHAGIATQNVVERLLLKEGKT
                     KYDLGREEFVKRVWEWKAHSGGQIKEQLKRLGCSLDWERERFTLDEGLSHAVRTVFVA
                     LHREGLIYQGYRIINWCPRCETALSDIETEYKDLAGKLYYIRYPYEHGDGCITVATTR
                     PETMLGDTAVAVNPGDSRYAHLVGKKVMLPLMERAIPIIADDFVDPAFGTGLVKVTPA
                     HDPNDFEMGKRHNLEEINIFNKNAIINENGGKYTGLDRYDARQKILEDLQDAGLLEKI
                     EEHNHAVGHCYRCHTVIEPYLSKQWFVTTEPLAREAIKVVEEGKIRFVPAQWEKVYFE
                     WMHNIKDWCISRQLWWGHRIPAFYCQDCGHIMVELEHPKECSMCHAKNIEQDPDVLDT
                     WFSSGLWPFSTFGWPEKTKALSKFYPTSVLVTGFDIIFFWVARMIMMGCKFMNDVPFK
                     DVYIHALVRDEHGQKMSKSRGNVIDPLIMMDKYGTDAFRFTLAIFAAQGRDIILSEKR
                     IEGYSAFCNKIWNSTRFICMNLGDDFVPSSFDINSLELFDKWILHALNQTIKTTTQAL
                     EEYRFNDAAQTLYDFWWHEFCDWYIELSKPRIYDNGAITGTIAKQVLYFVLHEYLKLL
                     HPFMPFITEEIWSTITTNEGRIVVAPWPQYNEDYNFENEYSDAELFKEVVYRIRNIRG
                     EMNVPPDKKGHVIIKAGKSIEQRLQGQAHNIKLLAKLETVDFNP"
     gene            complement(2443..3435)
                     /locus_tag="PLX22_03635"
     CDS             complement(2443..3435)
                     /locus_tag="PLX22_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19021.1"
                     /translation="MDKNGQLTFSDDPLLQGTNEMYMFIESGDFSLAVKKLDELLTIN
                     PEYPGLAESYRVARFWDSRKKNLDELSKGKETADFLMKQWEEFEAFAMDKNVVHTTAY
                     QKVMRYVFFTASEHYITAFNDRENPTDNLKLLMQLGTCLVRIAEYQHTIEVLEYARSQ
                     YQSNACILSLLAEAYYHVNDIPRSLLYFREAFLLNPQEIDLPLLSSKPIIEAVELTKQ
                     TKPDCKDIREWIPIVAFIHDIFYVKRQLNSSQLEQLKKDILRLEENYQTLNKDRVEQS
                     NIIPRLINRYLWMMDYYEFQHYDQQSLLDIRSRLLAIDKELFEGYFQKKSFSKP"
     gene            complement(3438..5540)
                     /locus_tag="PLX22_03640"
     CDS             complement(3438..5540)
                     /locus_tag="PLX22_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19022.1"
                     /translation="MKLLRSVISKIGFAENKQVDFNNNIHFIYGKNNSGKTLIAKSFI
                     DTLYPQEPSLIVADEWDTMFATFTIQSNLTNIAIQRKGNKEVKIFTATDGDTRAKELS
                     LQLPVVANSFSGDSHLERLHDLFNKDSFSLLSYIPSPLDNQYYAQNFSESIKSILLLD
                     RSLYYCSFKKIFFPTANQSGNYYEHIADEIQDKIKHCKTKLDLIELKEQRQRKALAEI
                     KALTDEITMHNEAIAIFQKKLKLLDEYIEILNAANNVAQQLQAVEQQIASLKEKLEKI
                     KKYEKLIAAKYPQFKNFSQEKRNNIHAMQTIYRQIQSVSEEIETCMFNHETKNHKRAR
                     NLLIINVMFAFGILFLFLKPLFNAIALNRWLLLAILIGIQVVSAGYYIIYTMTHSINK
                     LIAPLQQQKEEYQIKLETLLNQNNILLDSITIDEIYEFLLQYFEEYNEYNEKQVDLLT
                     IQMEIDEQKSLADLEQEKKEIESNYAFIKSQMDSFIQDIDYGHIETVEEAIAAKADIQ
                     HHCDEKKMMIQALQNKLKETPEEIADFNNEKLEVENEIHTLEKELYAIKEKKRVMTIV
                     KDSFNDAVERFYHDALTVLTHTVKTMYPELTGDQFPHIDEEYIKKALYTSFDSTAPAS
                     VKYLLYVTVVFGLYTMLQQQLPRIQLPLIIDDPFVLMDEDAIYNFTEVMQQWAHSRQI
                     IIFSHNSNIKKMYDVTEL"
     gene            complement(5537..6607)
                     /locus_tag="PLX22_03645"
     CDS             complement(5537..6607)
                     /locus_tag="PLX22_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19023.1"
                     /translation="MISILLMSDIHFGVDTAAPYIDDSIRQMTFNKICMLARQHTMFI
                     IAGDMFHAHVIDTPIFSFIINSLQALLDEGVKIVYVPGDAELANNDHPDRVLAVPSTV
                     TFYGNKNDYHTLLEFGSEVIHCYGSQLLIADQLHGIAREGLDGFHIGVFHVDLGDDNY
                     NNNGTIPVKSLKSMPLDFYALGHHHNFSMYKFKNRIVGAYPGSPEAVTPDEIGDRYVL
                     SITVENNEIHQIKRITVNSVKMETVTIQCDSVHSQDELSAILQRYNKSNIVLQCTLEG
                     MRSFDTTVISALCNQCAELVINDHSKPSSQFICNRYKQELSLRGYFYSELHTKIASES
                     PPYLQEIIDDIENTCSLFKGEL"
     gene            6979..7986
                     /locus_tag="PLX22_03650"
     CDS             6979..7986
                     /locus_tag="PLX22_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT19024.1"
                     /translation="MDSKGIHTIEDTKIDTTFKKANGIARIVGRDKAIVNLKNKIKQI
                     AKSKATVMILGESGTGKELIARAIHEESQRKNGQFIPVDCSTLTETIIESELFGHIKG
                     AFTGADKNKKGLIEEAHGGTIFLDEIGNLKPNIQSKLLRFLQEREIKPVGSSKTVKVD
                     VRIISATNINVEEEIAQGNFREDLYYRLSGIELTIVPLRQRIDDIPLLVEHFIAFYSG
                     DLDKDIQTASDDALEILMKREWKGNVRELQHIVEHAMIIEETSVLSKDTIIQILPEYK
                     QSVNHELSPLNLEKAVEAFEKKHIQYVLKLSHNNRSKTAKTLGISRSVLYEKLKKYGI
                     H"
     gene            complement(7989..>9049)
                     /locus_tag="PLX22_03655"
     CDS             complement(7989..>9049)
                     /locus_tag="PLX22_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19025.1"
                     /translation="GRLARGIAHEFNNILAGISGFTEILLRKIPQDDPNRHFAVRIYE
                     AASKAATLTNQLLGFARGQLFNPTYLDIRAPIMYAIGATPNLHSRITLIKQFEESDYR
                     VYGDVNQLRQVFIEVLRNAAEAIGDGGEITINVEYVSSDVLPVEIRKKYVRCVRVTIS
                     DSGSGIDESIIPKVFEPYFTTKDALNHSGMGLSIAFGHIKNHEGYIAIERMEKGTKVT
                     IVLPAIKEPDLTIIKQPRYQKILVIDDNNETCLLLRSYFEDRGVFVTVSNNGTGAIDM
                     IQNAPQRFDCIFLDLILPDINGKDVLSKIRSINPAVTVFVMSGYDMTAFINEHTGDQN
                     IFFLMKPFNLDDLDILLQ"
     assembly_gap    9050..9149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9150..10006)
                     /locus_tag="PLX22_03660"
     CDS             complement(<9150..10006)
                     /locus_tag="PLX22_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT19026.1"
                     /translation="MDTIYNSDLSLKELIDNLPDLVLSVDYPSGVIRYANIRAIERLG
                     FSTKKVHKLKDLARILLPRYRKKYLQFWRDLKEGILTPSITFEVKTLDNTTIWVEQKN
                     IYIKHNENVIRIHAVIRDITERKKLEEEMLQTFYFSENLFLHSPYIIVLFDKEGNITR
                     QNLTYRAMMEQVDNFIPGYNVLQDKQMQEYGIDLIFREVLRGRVMDVPRFPHTLTIKG
                     EEVTFYFAGKAFPYTRSSGDTEGFVAMLEDVTAKHKREQETLETKRLESVGRLARGIA
                     HEFNNILAG"
     gene            10393..10599
                     /locus_tag="PLX22_03665"
     CDS             10393..10599
                     /locus_tag="PLX22_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HOT19027.1"
                     /translation="MGRTLIVFGILFIIIGILLQLSPKLLGNLPGDVVFTRGKVTVYL
                     PFTTMIIISVILTIIANGIARLLK"
     gene            10749..11777
                     /locus_tag="PLX22_03670"
     CDS             10749..11777
                     /locus_tag="PLX22_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT19028.1"
                     /translation="MSKYSLQQVIERSKGWLKHPYSRIAFIIFIAISLYLIISAIVVI
                     VLTKPDKEVKIPDVVDKRYSDVHNSLIRKGLKPHLKFYDVHDIDNDIILRQYPEPGEI
                     VTENSTIRLLVSRSNLTIDMPSVVGMELPFALNKLKNLHLYDKTISLRVGVISYIPSE
                     KTADNVVIDQNPKSGQKIPLDKKVNLLVSSGSATQMVMPKVVGQSIDLCYDLLMSKKV
                     VVQYAIVPTNDISQSGIIASVTPSEGQPLTQGQVVTLQVYYYAMKDKPYYAYERLQYT
                     VPNDEGEGLYEVYVSDNKSKRLCFSQQLKPGNHIDCVFNRTGNARVYIMCNKKQVKVL
                     SFDVEEFD"
     gene            11758..12444
                     /gene="rpe"
                     /locus_tag="PLX22_03675"
     CDS             11758..12444
                     /gene="rpe"
                     /locus_tag="PLX22_03675"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT19029.1"
                     /translation="MSKNLTDTVLLSPSIIATDLSLLGSTVSSFDSTSIHFLHIDVMD
                     GNFVPNVTIGPGYIQNLKHHTSIPLDIHLMIAQPELSLIHYSECNPYFLTIHMESTRQ
                     PIRLLQQIKKAGIKAGLSINPSTPVAMLFDMIEYADMILIMSVEPGFYGQKFIPASLK
                     RIEQLHNFINANNMDVVIEVDGGINEDTIADVVKAGARIIVAGSGVFNAHDINKQAKV
                     LIQKACKALK"
     assembly_gap    12533..12632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12659..12901
                     /gene="rpsP"
                     /locus_tag="PLX22_03680"
     CDS             12659..12901
                     /gene="rpsP"
                     /locus_tag="PLX22_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT19030.1"
                     /translation="MVKIRLQRAGTKKKPFYKVVVIDSRKRRDGAVIESLGHYQPIVK
                     GDQFVVDRDKVTEWLKKGAQPTETIAKLLNKAGITG"
     gene            12931..13167
                     /locus_tag="PLX22_03685"
     CDS             12931..13167
                     /locus_tag="PLX22_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT19031.1"
                     /translation="MNYKELVEYMVKSLVDHPEQVEIKEIEGEKSTILELKVTKEDIG
                     KVIGKHGRIARAIRTILNASATKTGKRIVLEILD"
     gene            13164..13679
                     /gene="rimM"
                     /locus_tag="PLX22_03690"
     CDS             13164..13679
                     /gene="rimM"
                     /locus_tag="PLX22_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT19032.1"
                     /translation="MSSKEFFRIGVISGIRGFDGCLKVFIITDFPERFSGSSPVYVNI
                     HGHYKEFTIIDFTIAAKHTGYCYLEGIHDDVAAQALKGCDIFIDADDVQLYDDNSFHY
                     SVIIGCVVYYNNENWGTVVDILQTGAADVLVIKTYTGKEYFIPFVESMVSTQRLNEHI
                     LEIFPIEGLIQ"
     gene            13676..14377
                     /gene="trmD"
                     /locus_tag="PLX22_03695"
     CDS             13676..14377
                     /gene="trmD"
                     /locus_tag="PLX22_03695"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT19033.1"
                     /translation="MKIFKIITLFPDFFQSPLKSGLLGKAIEKGLCHIECINLRQWGE
                     GQYRQCDDYPYGGGSGMVLMPVPLLKAIESCKGDSKVLYPSPSGTVLNQDMVKELFHH
                     DSFLFICGQYEGIDQRVINRYVDYEISIGDYVLSGGEYAALVIIDALCRYIPGFMSNP
                     ESLSDESFENYLLEYPQYTRPREIDGMCVPDILISGDHEKIRRWRLEKSIEKTRATRP
                     DLYRLYIAKKDEVKQ"
     gene            14374..14715
                     /gene="rplS"
                     /locus_tag="PLX22_03700"
                     /pseudo
     CDS             14374..14715
                     /gene="rplS"
                     /locus_tag="PLX22_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            14811..15467
                     /locus_tag="PLX22_03705"
     CDS             14811..15467
                     /locus_tag="PLX22_03705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT19034.1"
                     /translation="MGEKPDFALEVHEYEKGCTIIGGIDEAGRGSLAGPLAVCMVIYP
                     CHFFANPPDDVIGVVNDSKKLSPKKRLAAKEIIITHCLWWECVLIPHDIIDEKNINGA
                     TYYAIVQLLQNNTMTPNMIFIDGNFSFPLPIPYQSVIKGDSLSLTIASASIIAKVTRD
                     EFMDKLDAEYPAYGFAHHKGYGTREHVQAIYHYGPCPVHRKTYQPVAGLLAGGLTLFK
                     "
     gene            15464..>15774
                     /locus_tag="PLX22_03710"
     CDS             15464..>15774
                     /locus_tag="PLX22_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19035.1"
                     /translation="MKITNNIVSAGLTQNSSLALKSGQVVVGTITRRMDTTTAMLRVG
                     SQLFETSFAKGLPKSNHILLRYDAMQGNRFIFTMVEPETMKHNDILLATTSNYRAFLH
                     SL"
     assembly_gap    15775..15874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15875..16288
                     /locus_tag="PLX22_03715"
     CDS             <15875..16288
                     /locus_tag="PLX22_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOT19036.1"
                     /translation="IQDVFSMMSKEDIESLLYYASDSSRNSYSIPFYDNNGFNNAYII
                     ADKDFVCIDVTFEHLGKLNISISKQKGLSIKIYCFNDGAYNALQPAMQQLSKTIEEAT
                     GEPAQCDCVLRKDWEEKIIALIHSMYIHYSIDFSV"
     gene            16317..16580
                     /locus_tag="PLX22_03720"
     CDS             16317..16580
                     /locus_tag="PLX22_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOT19037.1"
                     /translation="MEDIAVALEYLTEAKLPKIIAKGKGRLAKTILDKAKEHNIPIYQ
                     DTDLATVLLTLESSYIPEELYKAVAIVLAYCYNVNDRFRQKLN"
     gene            16669..>17779
                     /locus_tag="PLX22_03725"
     CDS             16669..>17779
                     /locus_tag="PLX22_03725"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT19038.1"
                     /translation="MRKIPVDELKPGMRFDKPVYIDSNNMLVAANVSIKIDDIKKLMR
                     WGVSEVETAGTLVSTEQEIKAATARATMTDSDQARKIIDEYNKLLTQRVPLIEIHREA
                     CKAVGNVYSAIKADEPFTIDEIDAALSKIIEIMKNYPNIFLFLYGLEEGKNYLVVHSV
                     NVTFYAILIGMALKYTPIKLRELGLGTMLIDAGMIKLPAYIMHKQSNLTEHEFNQIKT
                     HPLHGYKALRQLGKIPEKSALICLQHHEQFDGKGYPRGLKGTQIDEYARIAAIADSYE
                     AQIANRVYRKRIFFYQAMKNLLSSGINKFDTVILRVFLSRMSVYPIGSIVELSNGTIG
                     IVIGSVPQKPLRPIIKLIMDQNKKRFTDTIIVNLLE"
BASE COUNT         5513 a         3337 c         3210 g         5419 t
ORIGIN      
        1 ggggttaaag tctactgttt ctaacttcgc cagtaacttt atattatgcg cctgtccctg
       61 taaacgttgt tcaatggatt tacctgcctt aataattaca tgccccttct tatctggcgg
      121 aacattcatt tcgccgcgga tattacgtat tctatacacc acctctttaa ataattcagc
      181 atcactatat tcgttttcaa aattataatc ctcattgtac tggggccacg gagcaactac
      241 tattctcccc tcattggttg taatagtact ccatatctcc tctgttataa atggcataaa
      301 gggatgtaat aatttcaaat actcatgcaa aacaaaatac aatacctgtt tggcgatagt
      361 tccggtaata gcaccattgt cataaatcct tggtttggaa agttcaatat accaatcaca
      421 gaactcatgc caccagaaat catacagcgt ttgagcagca tcattgaaac gatattcctc
      481 cagtgcttga gttgttgttt taatagtctg gttgagtgca tgcagtatcc atttatcaaa
      541 aagctctaac gaattaatat cgaatgaaga ggggacaaaa tcatcaccta aattcataca
      601 aataaagcgt gtgctattcc atattttatt gcaaaatgcg ctgtagcctt caatacgctt
      661 ttccgacagt atgatgtctc tgccctgagc agcaaagatt gccagagtaa atctaaaggc
      721 atcagtgcca tatttatcca tcattataag cggatcgata acattgccac gtgatttact
      781 catcttctgc ccatgctcat cacgtaccag tgcatggata taaacatcct tgaaggggac
      841 atcattcata aacttgcacc ccatcatgat catgcgtgcc acccagaaaa agataatatc
      901 aaagcccgta accagcacgc tggtagggta aaattttgac aatgcctttg tcttttctgg
      961 ccagccaaag gtagaaaagg gccacaatcc cgaggaaaac catgtatcta aaacatccgg
     1021 gtcctgctca atattttttg catgacacat actgcattct ttgggatgtt ctaactcaac
     1081 catgatatgt ccgcaatcct ggcaataaaa tgctggaatg cggtgacccc accacagctg
     1141 gcgggaaatg caccagtcct tgatattatg catccattca aaatatacct tttcccattg
     1201 tgccggaaca aatcgtattt tcccttcctc aactaccttt atagcttcac gagccagtgg
     1261 ttctgttgta acaaaccact gtttggaaag atacggttca ataacagtat gacatcggta
     1321 gcaatgccca acagcatggt tatgctcctc aattttttct aaaagtccgg catcctgtag
     1381 gtcttccaga atcttctggc gagcatcata ccggtcaagt cccgtatact ttcctccatt
     1441 ttcattgata attgcatttt tattgaagat attaatctct tcaaggttat ggcgtttacc
     1501 catctcaaag tcattgggat catgggcagg ggttacttta acaagcccgg tgccaaatgc
     1561 tggatcgaca aaatcatcag caataatggg gattgctctt tccatcaacg gcaacataac
     1621 ctttttgcca accagatgcg cataacgact atcgccagga ttaacagcaa cagcagtatc
     1681 acccagcatg gtttcaggcc gtgttgtggc aacggtgata cagccatcac catgttcata
     1741 gggatagcgg atataataca gcttacctgc taaatccttg tattcggttt caatatcact
     1801 taacgctgtc tcgcaacgcg ggcaccagtt tataatacga tatccctgat agattaatcc
     1861 ttccctgtgc aatgcaacaa acaccgtgcg gactgcatgt gacaaacctt catccagcgt
     1921 aaaacgttca cgctcccagt ctaatgaaca tcccagacgt ttcagttgct cttttatctg
     1981 tccaccagaa tgcgctttcc attcccagac acgttttaca aattcttctc tgcctaaatc
     2041 atattttgtt ttaccctctt ttaataaaag gcgttcaaca acattctgtg tagcaatccc
     2101 tgcatggtcc atacccggca tccagcaggt agctttccct ttcatccttt gccagcgaat
     2161 gagtatgtcc tgcaacgtat tgtttaatgc atgtcccatg tggaggttac cggttacatt
     2221 gggcggcggt attactatag aataggcatc gcggggatca ttttcatcac cgtgaaaaag
     2281 tccttcctgt tcccacactg tataccatgt agactcggca atctttggat tatacgatgc
     2341 atgtaattcc atactccacc acctgtaatc tattctgttt tgctggtagt taattattca
     2401 gcagtatatg tagtgtcaat gaattttttc agattgtcat tttcatggtt ttgaaaatga
     2461 ctttttctgg aaataacctt caaaaagctc tttatcaatg gcaagcaaac gactgcggat
     2521 atccagcaaa ctttgctgat catagtgctg aaattcataa taatccatca tccacaggta
     2581 gcggttaatc aagcgtggga taatatttga ctgctcaact ctatccttat tcaaggtttg
     2641 atagttttct tctaatctca aaatatcctt ctttagctgt tcaagctgtg atgaattgag
     2701 ctgccgctta acataaaata tatcatgtat aaatgcaaca attggtatcc attcgcggat
     2761 atccttgcag tcaggttttg tttgtttggt taactcaact gcctcgatga ttggttttga
     2821 cgatagcaat ggcaagtcaa tctcctgtgg attaagtaaa aatgcttccc tgaaatagag
     2881 caatgatcgc gggatatcgt ttacatgata gtacgcctcg gctaaaagcg acaatatgca
     2941 tgcattactc tggtactgtg agcgtgcata ttccagcacc tcaatggtat gctgatattc
     3001 agcaatacgc acaagacagg tacctaattg cattaaaagt ttcaggttat cagttggatt
     3061 ttcccgatca ttgaatgcag tgatatagtg ttcggaagca gtaaaaaaaa catagcgcat
     3121 gaccttttga tatgctgttg tatgaactac atttttatcc atggcaaatg cttcgaattc
     3181 ttcccactgt ttcattaaaa aatctgctgt ttcctttccc tttgataact catccagatt
     3241 tttttttctt gaatcccaga accgtgcaac acgatatgat tcagcaagtc cgggatattc
     3301 cggatttatt gttaaaagct catccagctt ttttaccgca aggctaaaat cccctgactc
     3361 aatgaacata tacatttcat tggttccctg gagtaaagga tcatccgaaa atgttaattg
     3421 cccgttttta tccatagtta caattcagta acgtcataca ttttttttat attagaatta
     3481 tgggaaaaaa tgataatctg ccgggaatgt gcccattgtt gcattacttc tgtaaaatta
     3541 tagattgcat cttcatccat taaaacaaaa ggatcatcaa taatgagcgg taattgtatc
     3601 cggggtaatt gctgctgcaa catggtgtat aatccaaaca caacagtgac atagagcaaa
     3661 tatttaaccg atgcaggcgc tgtactatcg aaagaagtat ataacgcttt ttttatatat
     3721 tcttcatcaa tgtgtggaaa ctggtcccct gtaagttctg gatacattgt tttaacagta
     3781 tgcgtcaaca ctgttagtgc atcatggtaa aaacgctcaa cagcatcatt aaacgagtcc
     3841 ttaacgattg tcataactcg ttttttttct ttgatagcat ataactcctt ttccagggtg
     3901 tgtatttcat tttcaacttc caatttttca ttgttgaaat cagcaatttc ttcaggtgtt
     3961 tcttttaatt tattttgcaa tgcttgaatc atcatctttt tttcatcaca atgatgttga
     4021 atatcggctt ttgcagcaat agcttcttca acagtttcaa tatgcccata atcaatatcc
     4081 tgaataaaag aatccatctg actttttata aaagcataat tactttctat ttctttcttt
     4141 tcttgttcaa gatcagctaa tgatttttgc tcgtctatct ccatttgtat ggttaacaaa
     4201 tcaacctgtt tttcattgta ttcgttatac tcttcaaaat attgtaaaag aaattcataa
     4261 atttcatcaa tggtaatact atcaagtaga atattattct ggttgagaag cgtttccaat
     4321 tttatttgat actcttcctt ctgttgttgt aagggtgcaa tcaatttatt aatagagtgt
     4381 gtcatggtat aaataatgta atagccagca cttacaacct gaatccctat aagtatagct
     4441 aacaataacc acctgttcaa tgcaattgca ttaaacagtg gcttcagaaa taaaaataat
     4501 ataccaaatg caaacattac atttataatt aaaagatttc gagcacgttt atgattttta
     4561 gtttcatgat taaacatgca ggtttcaatt tcttcagata ccgattgtat ctgccggtat
     4621 atagtttgca ttgcatgaat attgtttctt ttttcctggg aaaaattttt aaattgcgga
     4681 tattttgctg caatgagctt ttcatatttt tttatttttt ctaatttttc tttaagtgat
     4741 gctatttgtt gctccacagc ttgcaactgc tgtgctacat tatttgccgc attaaggatt
     4801 tcaatatact catccagcaa ttttaatttt ttttggaata tagctatcgc ctcattatgc
     4861 atcgtaattt catcggtaag cgctttgatt tctgcaagtg cctttctttg acgttgctct
     4921 ttaagttcaa tgagatctaa ctttgtttta caatgtttaa ttttatcctg aatttcatct
     4981 gctatatgtt cataataatt tccggattga tttgccgtag gaaaaaatat ctttttaaaa
     5041 gaacaataat acaatgatct gtctaataaa agaatggatt ttatgctttc agaaaaattc
     5101 tgcgcatagt actggttatc cagtggtgaa ggaatatatg acaataatga aaacgaatcc
     5161 ttattgaaaa ggtcatggag cctttcaaga tgactatcgc cactaaaact atttgccaca
     5221 accggaagct ggagcgatag ttctttagca cgtgtatcac cgtcggtagc agtaaaaatt
     5281 ttaacttctt tattcccttt ccgctgtatc gcaatatttg tcagattaga ttggatggta
     5341 aatgttgcaa acatcgtatc ccattcatca gcaacaatga gggatggttc ttgcggataa
     5401 agcgtgtcta tgaaactttt tgcaataagc gttttacccg aattattttt accataaata
     5461 aaatggatat tattattaaa gtcaacttgt ttattttcag caaaaccaat ttttgatatg
     5521 acacttcgta gcagcttcac agctcacctt taaataaaga acacgtattt tcaatatcat
     5581 ctataatttc ctgcaggtag ggaggggatt cactggcaat tttagtatgc aactcagaat
     5641 aaaaatagcc gcgaagcgat agttcctgtt tatacctatt acagataaat tgactggaag
     5701 gtttagaatg atcgtttatc accagttcag cacactggtt gcacagagca gaaattactg
     5761 tagtatcaaa tgagcgcata ccttccagtg tacattgcaa cacaatattg gatttattat
     5821 agcgttgtaa aatagctgat agctcatcct gtgaatgaac agaatcgcat tgaattgtta
     5881 ctgtttccat ctttaccgag tttaccgtta tacgctttat ctgatgaatt tcattatttt
     5941 caacggtgat tgagagcaca tatctatcgc ctatttcatc aggcgttaca gcctcaggac
     6001 tgcctggata agcgccaact atcctatttt taaatttata cattgaaaaa ttatgatggt
     6061 gtcccagtgc ataaaaatca agtggcattg attttaatga ctttaccgga atagtaccgt
     6121 tattattgta attatcatca cccagatcaa catgaaacac tccaatatgg aagccatcca
     6181 aaccttccct ggcgatgcca tgcaactggt cggcaataag caattgagac ccgtaacaat
     6241 ggataacctc actcccaaat tccaatagag tatgataatc attcttatta ccatagaagg
     6301 ttaccgtaga tggaacggca agcaccctgt caggatgatc attatttgcc aattctgcat
     6361 cacctggaac ataaacaatt tttacccctt catcaagcag tgcttgcaat gaattgataa
     6421 tgaaggaaaa aataggtgta tcaataacat gggcatggaa catatctccg gcaataataa
     6481 acatggtatg ctgacgggca agcatacata ttttattaaa tgtcatttga cgtatgctat
     6541 catctatata tggtgcagca gtatcaacac caaaatggat gtcagacatt aacagtatag
     6601 aaatcattac ttatatatca cttaaaaata gaatgtatgc tacatctata taacgttata
     6661 ctccacatcc cataaggcag aatttacaac tatatatatt accatgatga gcattttatc
     6721 ttccatttat gaaatataat tgcaaaatac tgtacatcgg tcaaacatta tttaaaaaaa
     6781 atgattgtat atttatcata aattgttgat agtgtcatgt gaagcataga aatggaataa
     6841 tcttttgatt gtttcaaaca gcgtcatcat gatgtaactt cttgagcatg agaaatacta
     6901 actactatca tagcgatacg tatatgaaat aaattgtaat agtagtaaat tgctttaatt
     6961 gcgttattga agaaaaaaat ggacagcaag ggcatacaca ctattgaaga cacaaagata
     7021 gatacaacct ttaaaaaagc taatggtatt gcgcgtattg ttggcaggga taaagctatt
     7081 gtcaatctaa aaaataagat taagcagata gctaaatcaa aagcaaccgt tatgattctt
     7141 ggtgaaagcg gcacaggcaa agagctcatt gcacgtgcaa tccatgaaga aagtcagaga
     7201 aagaatggac aatttatccc tgttgattgc agtacattga cagaaaccat cattgaatca
     7261 gaactatttg gacatataaa gggagcgttt accggagctg ataaaaataa aaaagggctg
     7321 atagaagaag ctcatggtgg tactatattc ttagatgaaa ttggcaacct taaacccaat
     7381 attcaaagca agttgcttcg ttttttacag gaaagggaga tcaaaccggt tggttcaagt
     7441 aaaaccgtaa aggtggatgt tcgcatcata agtgctacca acattaatgt ggaagaagag
     7501 attgcacagg gaaatttcag ggaagatctc tattaccggt tatccggtat tgagctcact
     7561 atagtaccgt tacgccagcg tatagacgat atcccgcttt tagtagagca ttttattgcc
     7621 ttctattccg gagaccttga taaggatatc caaactgcca gcgatgatgc tctcgagatt
     7681 ttgatgaaac gtgaatggaa aggcaatgta cgggaattac agcatattgt agagcatgca
     7741 atgatcatcg aagaaacatc agtgctgtca aaagatacta taatccagat tttacctgaa
     7801 tacaaacaat cagttaacca tgaactatcg ccattaaacc ttgaaaaagc cgttgaagca
     7861 tttgaaaaaa aacatataca gtacgtgctt aaactatcgc acaataaccg ctcaaaaaca
     7921 gcaaaaacac tgggtataag ccgaagtgtt ttatatgaaa aattaaagaa gtatggcata
     7981 cattagtatt actgcagcaa tatatccaga tcatcaaggt taaaaggctt catcaaaaag
     8041 aaaatatttt gatcaccggt atgttcattt ataaaagcag tcatgtcata cccgctcata
     8101 acaaatactg tcactgctgg attaatactt ctgattttac ttaaaacatc tttcccgttt
     8161 atatcaggca aaataagatc aagaaaaata caatcaaacc gttgcggagc attttgaatc
     8221 atatcaatag cacctgttcc gttattggat acagtaacaa atactcctct atcctcaaaa
     8281 tatgatcgca gcaataagca tgtttcatta ttatcatcaa tcaccaatat cttttgatat
     8341 cgtggctgct ttattatagt aagatcaggt tcttttatag caggtaaaac aatagttacc
     8401 tttgtccctt tttccatgcg ctcaatagct atataccctt catgattttt tatatgtcca
     8461 aatgcaatcg acaatcccat ccctgaatgg tttaatgcgt cttttgtggt aaaatacggc
     8521 tcaaacactt taggaataat tgactcatct attcctgatc ctgaatcgga tattgtaaca
     8581 cgcacacacc gtacatattt tttcctgatt tccacaggca aaacatcact gctgacatac
     8641 tcaacgttaa tagttatttc acctccatca cctattgctt cagcagcatt ccgtaacacc
     8701 tcaatgaaca cctgccgcag ctgattgaca tcaccataca ccctgtaatc ggattcctca
     8761 aattgcttta taagggttat cctgctatgc aaattgggtg tagcacctat tgcatacata
     8821 attggcgcac gtatatcaag gtaggttggg ttaaaaagct gtccccgggc aaaacccaat
     8881 aactggttgg taagggtagc ggctttgctt gccgcttcat aaatccttac tgcaaaatgg
     8941 cggttgggat catcctgtgg aatcttccgt aataaaattt cagtgaagcc tgaaatacct
     9001 gcaaggatat tattaaattc atgagctatc cccctggcaa gccttccaan nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tacctgcaag gatattatta aattcatgag
     9181 ctatccccct ggcaagcctt ccaacacttt caagccgttt tgtttccagc gtttcctgtt
     9241 cccttttatg ttttgcagtt acatcctcca gcattgcaac aaatccttca gtatccccgg
     9301 aggagcgagt ataggggaac gccttaccag caaagtaaaa ggttacctct tcccctttta
     9361 ttgttagggt gtgaggaaag cgtggaacgt ccattaccct gccgcgtaat acctccctga
     9421 aaatgagatc aatcccatat tcctgcattt gtttatcctg caagacatta tagccgggta
     9481 taaaattatc aacctgttcc atcattgccc tgtaggtaag gttttgccgt gtaatatttc
     9541 cttctttatc aaagagcact attatatacg gtgaatgcaa aaaaagattt tcagaaaaat
     9601 aaaacgtttg cagcatctct tcttctaatt ttttccgctc ggttatatca cgtatcacgg
     9661 catgaatacg gataacattt tcattgtgct ttatataaat atttttttgc tcaacccaga
     9721 ttgtggtatt atccaatgtt tttacttcaa aggtaataga gggtgtaagt atcccctctt
     9781 ttagatcacg ccaaaattgt agatattttt ttctgtagcg aggtaacaaa atacgggcta
     9841 aatccttgag cttgtgcacc ttttttgttg aaaaacccag cctttctatt gcacgtatgt
     9901 tggcatagcg gataacacct gaaggataat ctaccgataa tacaagatcc ggcagattgt
     9961 caataagctc cttcagactc aaatcgctgt tatatatagt atccatgttg ttcataggtt
    10021 agccgttata aactaccttt cccattgtat tgataaccag tgaaaagctt ctctctggca
    10081 taaaatcata caccattgac attctgagat acgcagcttt tgtcgttctg agggagcatt
    10141 aacacccaaa gaatctattt gttcaacggc tacattcttt actttgactt atatcagcac
    10201 aggcagatta gcctcagaat gacacatcac aacacgattc tttgctattg ctcagattga
    10261 cataaaacac tattaatgct atattattaa tacattgtca atgtcaataa ttattatatc
    10321 atgcgattaa tcataccaaa tgatggcatg agctgctttg gcagtattat tttttttaat
    10381 aaaaggaatg ttatgggcag aacgcttatt gtgtttggga tacttttcat catcatcggg
    10441 attttattac aactatcgcc aaaattattg ggtaatttgc ccggcgatgt tgtatttacc
    10501 cgaggcaaag taacggtata cctaccattt accactatga ttatcatcag cgttatactt
    10561 actattattg ccaatggcat tgcgcgatta ttaaaatgat tgtatgagtt attcactttt
    10621 ttactaattg ctatagtaaa ataattcttg acgcattatg taaattttgc ttgtgttcaa
    10681 ttttccattc tataatagta acaaaaatag taacaattat cttttttaca tcgcagaggg
    10741 atgttaccat gtctaagtat tcgttgcaac aagttattga acgctcaaaa ggctggctta
    10801 aacaccccta tagccggatt gcctttatca tctttattgc catttcacta tacctgatta
    10861 tttcggcgat agttgttatt gtattgacca aacctgataa ggaagttaaa atccctgatg
    10921 tcgttgacaa acgttattca gatgtgcaca acagtcttat ccgtaaggga ttaaagcccc
    10981 acctaaaatt ttatgatgtt catgatattg ataacgatat cattttacga cagtatcctg
    11041 aaccagggga gatagtaacc gaaaatagca ccatacgtct attggtaagt cgcagcaacc
    11101 tgacaataga catgccaagc gtggtgggga tggaattacc gtttgctctc aacaaactaa
    11161 aaaatcttca tctgtatgat aaaaccattt cactacgagt tggtgtaata tcctatatcc
    11221 cttcagaaaa aaccgctgat aatgttgtca tagatcaaaa tccaaaatcc ggtcaaaaga
    11281 tcccactgga taaaaaggtt aatctgttag tttcaagcgg cagcgcaaca cagatggtaa
    11341 tgcccaaagt agttgggcaa tcaattgatt tgtgttatga tcttttgatg tcaaaaaagg
    11401 ttgttgtaca gtatgctata gtacctacta atgatatatc gcaaagtggt attattgcat
    11461 cagtaacacc ttctgaaggg caaccgctta cccaggggca ggttgtaaca ctgcaagtat
    11521 actattatgc aatgaaagac aaaccgtact atgcctatga acgcctgcaa tacaccgtcc
    11581 ccaatgatga aggtgaaggt ttatacgagg tttacgtaag cgataataaa tcaaaacgcc
    11641 tgtgcttttc acagcagttg aaaccgggta accacattga ttgcgtattc aaccgtaccg
    11701 gcaatgcacg ggtatatatc atgtgtaata aaaaacaggt aaaggtgctt agctttgatg
    11761 tcgaagaatt tgactgatac cgtattactg tcgccatcaa tcattgccac cgatctttct
    11821 ttgctgggtt ctactgtatc atcgtttgac agcacttcta tacatttttt gcatatcgac
    11881 gttatggatg gtaattttgt tcccaatgtc accatcggtc ctggctacat tcaaaacctg
    11941 aaacaccata ccagcatacc acttgatata cacctgatga ttgctcaacc ggaactatcg
    12001 cttatacact atagtgagtg taatccctat tttttaacaa tacacatgga aagtacccgt
    12061 caaccaatac gattgctgca acagataaaa aaggctggaa taaaagccgg tttaagtatc
    12121 aacccctcta cacctgttgc aatgttgttt gacatgatag aatatgctga tatgatattg
    12181 attatgtcgg ttgagccggg attttacggg cagaagttta ttcccgcttc attaaaaaga
    12241 attgaacagc ttcataattt tattaatgct aacaatatgg atgtagtcat tgaggttgat
    12301 ggcggtatca atgaggacac catagcagac gttgttaaag caggtgcccg aatcatcgtt
    12361 gccggcagtg gcgtttttaa tgcacatgat atcaataaac aggcaaaggt tcttatacaa
    12421 aaagcctgta aagcccttaa ataatggtaa taatttttgt gcttgcagtg gaatgtgcta
    12481 atacactact atatatttct attgacaaaa tatgcttata aaaaataatc atnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncactaatt acttcaggag gtagataggt
    12661 ggtaaagatt cgattgcaac gtgcgggaac taagaaaaaa ccattttaca aggttgtggt
    12721 gattgattca cgaaagcgtc gtgatggtgc tgtcattgaa agtcttggac attatcaacc
    12781 aatagtgaaa ggtgatcaat ttgtggttga tagagacaaa gttactgagt ggcttaaaaa
    12841 aggcgctcaa ccaacggaaa ctattgcaaa attattgaac aaagccggaa ttacaggcta
    12901 atacgatttt gatatgtgga ggcagtaacg atgaattaca aggagcttgt tgagtatatg
    12961 gtaaaatcgc ttgttgatca tcctgaacag gtggaaatca aggagattga aggtgaaaaa
    13021 tctactatcc ttgagttaaa ggttaccaag gaagatattg gcaaggttat tggcaaacat
    13081 ggaaggattg ctcgtgctat tagaaccata ttaaatgctt cggctacaaa aacaggtaaa
    13141 aggatagttc tggaaatact tgattgagca gtaaagaatt tttccgtatt ggtgttatct
    13201 ccggcatccg tggttttgat gggtgcctta aagttttcat aatcactgat tttcccgagc
    13261 gtttttctgg cagttcaccg gtatatgtta atatacatgg gcactataag gaatttacca
    13321 taattgattt tactattgca gcaaagcata caggctactg ctatttagaa ggaatacatg
    13381 atgatgtagc cgcacaggcg ttaaagggat gtgatatttt tatagacgct gatgacgtcc
    13441 agttgtatga tgacaatagt tttcattatt ctgttataat agggtgtgtt gtttattata
    13501 acaatgaaaa ctgggggacg gttgttgata ttttacaaac aggtgcagcc gatgtactgg
    13561 taataaaaac gtacaccggg aaggaatatt ttattccttt tgttgagagt atggtgagca
    13621 cacaacgact taacgagcat atccttgaga ttttccctat tgaagggttg attcagtgaa
    13681 gatctttaaa attataacgc tatttcccga ttttttccag agtccactga aatcaggact
    13741 gttagggaaa gcaattgaaa aaggtttatg ccatattgaa tgtatcaatc tacgacaatg
    13801 gggtgaaggg cagtacaggc aatgcgatga ttacccctac ggcggcggca gcggcatggt
    13861 gctgatgccc gtccctttat tgaaagctat tgaatcatgt aaaggtgaca gcaaggtgct
    13921 ctacccttca ccatcgggaa ctgttttgaa tcaggatatg gttaaagagc tttttcacca
    13981 tgacagcttt ctttttatat gcggtcagta tgagggcatt gaccagcgcg tcatcaaccg
    14041 ctatgttgat tatgaaatat caataggcga ttatgtcctt tctggtggtg aatacgcagc
    14101 gctggtaatt attgatgctt tatgtcgtta tatcccggga ttcatgtcca atcccgagtc
    14161 actatcggat gaaagttttg aaaattatct tttagaatat ccacagtaca cccgacctcg
    14221 ggaaattgac ggtatgtgtg tgccggatat tctgattagc ggtgatcatg aaaaaatacg
    14281 cagatggcgt ttagaaaaga gtattgaaaa aaccagggct acaaggcctg atctctatag
    14341 attgtatatt gcaaaaaaag atgaggtgaa acaatgaaag caatacaatt aattcagaat
    14401 gaattaagta aaagttaccg tgatgtcaat tttgaagtgg gcgacactgt taaggtccac
    14461 tataaaatta ttgaaggtga caaagaacga attcaggttt acgaaggtgt ggttattgca
    14521 attgataata aaggcataag caaaactttt accgtgcgac gtatttccta tgatgttggc
    14581 gttgaaagaa tatttcccct gcactcacca cgaattgaca aaattgttgt aatgcgcaag
    14641 gggaaaaaac gcagagcaaa actttatttt ttacgcgagc gaactggtaa atctgcaaaa
    14701 cttagagaag tacgtgttca taaaaaaggg gttgaaactc ccactaccca tgaagctatt
    14761 gacgctcaaa aggaaagcac aaaatctaat gtataaataa taattatact atgggtgaaa
    14821 aacctgattt tgcattagaa gttcatgaat atgaaaaagg ctgtacaata ataggcggca
    14881 ttgatgaagc aggaagaggc tctttagcgg ggcctcttgc tgtttgtatg gttatttacc
    14941 cctgccattt ttttgctaac ccccccgatg atgttatagg tgttgtcaat gattcaaaaa
    15001 aattatctcc caaaaaaaga ttggctgcaa aagaaataat tattacacat tgcctgtggt
    15061 gggaatgtgt tctaatacct cacgatataa tagatgaaaa gaatattaat ggtgcaacat
    15121 attatgccat tgttcagctt ttacaaaaca ataccatgac acctaatatg attttcatag
    15181 atggtaattt ttcgtttcca ctgcccatac catatcaatc agttataaaa ggtgatagcc
    15241 tttcgcttac cattgcatca gcatcaatca ttgcgaaggt aacccgtgat gaattcatgg
    15301 ataagcttga tgctgaatat cctgcatatg gttttgcaca tcacaaggga tatggcaccc
    15361 gtgaacatgt acaggctata tatcactatg gaccctgccc tgttcaccgt aaaacctatc
    15421 aacctgttgc aggattactt gcaggaggat taacactttt taaatgaaaa ttaccaataa
    15481 cattgtcagt gcaggattga cgcaaaactc atcacttgcg ctgaaaagcg gacaggttgt
    15541 tgttggcaca ataaccagac gaatggatac tacaacagcg atgctgcgtg taggaagcca
    15601 gctttttgaa acaagctttg caaaaggatt gccaaagtca aaccacatac tactgcgata
    15661 tgatgcaatg cagggcaacc gatttatatt taccatggta gagcctgaaa caatgaaaca
    15721 taatgatata ttgcttgcaa ccacctcaaa ctacagagca tttttgcatt ctctnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatacag gatgtttttt caatgatgtc
    15901 aaaagaggat attgaaagtt tactctatta tgcaagcgat agttcaagaa atagctattc
    15961 catccctttt tacgacaaca atggatttaa caatgcatat attattgccg ataaggattt
    16021 tgtatgcata gatgtaacct ttgaacatct tggtaaactt aacattagca tcagcaaaca
    16081 aaaggggctt tctataaaaa tctattgttt taatgatggt gcttataacg ctttacagcc
    16141 tgctatgcaa caactatcga aaacaataga agaagctaca ggtgaacctg cacaatgcga
    16201 ttgtgtattg cgcaaagact gggaagaaaa aataattgca ttaattcata gtatgtatat
    16261 acactacagc attgattttt ctgtataaag taaatgtact tttggggaac aatatcatgg
    16321 aagatattgc ggtagcacta gaatacttaa ccgaagccaa actgcctaaa attatagcca
    16381 aaggaaaagg cagattagct aaaacaattc ttgacaaagc taaagagcat aatataccta
    16441 tttatcagga tactgattta gccacggtcc ttttgaccct tgaaagttca tatattcctg
    16501 aagagctcta taaagcagtt gcgatagttc ttgcatattg ttataatgtt aatgatcgtt
    16561 ttagacaaaa attaaattga gtgtagcagg tataccaatt tttatatttt ttaatatttt
    16621 gtacttgatt acttcaattg aaacaattac accattgata tggttcagat gagaaaaata
    16681 ccagtagatg aacttaaacc tggcatgagg tttgacaaac ctgtttacat tgacagcaat
    16741 aatatgcttg tagcagccaa tgtatccatt aagatagatg atataaaaaa gctcatgcgc
    16801 tggggagtaa gcgaggttga aactgcaggg actctggttt ccacggaaca ggaaataaaa
    16861 gctgcaacag ctcgcgcaac catgaccgac agcgaccagg caaggaaaat aattgatgaa
    16921 tataacaaat tattaacaca gcgtgtccca ctcattgaaa tacaccgtga agcgtgcaaa
    16981 gcagtaggga atgtttatag cgcaattaaa gctgacgaac catttaccat tgatgaaatt
    17041 gacgctgcat taagcaagat cattgaaata atgaagaact atcccaatat atttttattt
    17101 ttatatggat tggaagaagg caaaaactat cttgtagtac attcagttaa tgttaccttt
    17161 tatgcaatct taattggtat ggcattaaaa tatacaccaa tcaaacttcg tgagcttggt
    17221 ttagggacca tgcttattga tgctggcatg attaaactgc cagcgtatat aatgcataaa
    17281 caatctaact taaccgagca tgaatttaac cagataaaaa cccatccact gcatggatac
    17341 aaggcattgc gccagcttgg taaaatacct gaaaaaagcg ctcttatctg cctgcagcat
    17401 catgaacaat ttgatgggaa agggtatcca cgggggctta aaggaactca gattgatgaa
    17461 tacgcacgta ttgccgcaat tgctgattcg tatgaagcac agattgcaaa tagggtatat
    17521 cgtaaacgga tattcttcta tcaagcaatg aagaatctgc tttcaagcgg tatcaataaa
    17581 tttgatacgg ttattttgcg ggtattttta tcaaggatgt cagtatatcc cattggttca
    17641 atagttgaac tcagcaatgg aaccataggt atagtgattg gttccgttcc gcaaaaacca
    17701 ttgcgtccta ttatcaagct cattatggat caaaataaaa agcgattcac tgataccatt
    17761 attgtcaacc tattagaag
//
LOCUS       DAOSOX010000029        17358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3296_length_17358_cov_8.096573, whole genome shotgun sequence.
ACCESSION   DAOSOX010000029 DAOSOX010000000
VERSION     DAOSOX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3296_length_17358_cov_8.096573"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLX22_03730"
     CDS             <1..834
                     /locus_tag="PLX22_03730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HOT19039.1"
                     /translation="RYCSPILEKGDLVIVISQSGETADTLAAMREAKARGVRVLGITN
                     VVGSTIARESDGGVYIHAGPEIGVASTKAFTSQITVLILLTFYLARRRFMTADAGREL
                     LQELIQVPALVQSILGKNDEIKEIATLYQHHRNFLYLGRGIQFPVALEGALKLKEISY
                     IHAEGYPAAEMKHGPIALIDENMPVVFIAIKDEVYQKVISNMQEVKARKGKIIAIATE
                     GDEEINKFADHVFYVPATVKIASPLLTVIPLQLLAYHIAVLRGCNVDQPRNLAKSVTV
                     E"
     gene            994..1368
                     /locus_tag="PLX22_03735"
     CDS             994..1368
                     /locus_tag="PLX22_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT19040.1"
                     /translation="MIVIEDIDYIGVTVSDMDNAISFYSDLFDFEVIERVSNTDEAYI
                     RVGDILLKLVEVKDFKAPENLKNNICFYIDEEDFDDALDELQEKNITIVYGPENIRKG
                     RLVVFTDPDGNKIELRYPRFSL"
     gene            1390..2016
                     /gene="eda"
                     /locus_tag="PLX22_03740"
     CDS             1390..2016
                     /gene="eda"
                     /locus_tag="PLX22_03740"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOT19041.1"
                     /translation="MSLQFSIPIIPIGVFYDVARTLKMFELLMQHGIRCIEVTLRTAQ
                     ALDCITALKNTFPDAMIGAGSVITHDDFKAAVDAGAMFIVSPCVSDSLIELSHKNKNI
                     YYIPGFTTPTELAHALQMHVDVLKFFPAEYCGGVHYLKAIVEPFAQFSFSIIPTGGIK
                     PAHIAEYLSLPKVVACGLSYIVDSYLIEHGDYINLEKRINEILACLQR"
     gene            2017..2967
                     /locus_tag="PLX22_03745"
     CDS             2017..2967
                     /locus_tag="PLX22_03745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19042.1"
                     /translation="MSIYKNIETAIYKRENLLKQTITNAFRLVNGQGDGCYGLILDYY
                     DDYLLLQVYDSSLYQEYHRISDHCIKILACCNRAIKGVLLKDRSKVNDPQKIAESRKS
                     VCITGTLPPVPLIAKQNDIVAGVDLINGQNTGLFLDMRGVRQRLAPYYANGGKLCNLF
                     SYTGIFAIHALKNGLHHAVNVDMSKSVLYRAKQNYMLNHIKDDNRDFVAMDCKKFLQY
                     AAKHNDTFHFVIFDPPTFSRNKGHNFSVKKHYNEFLSLIAPIVSDGYILTTINTISIS
                     DDEYISLHPGNWELQFLEHEPDDFPYYKEPYLKAGLWKMR"
     gene            3081..3746
                     /locus_tag="PLX22_03750"
     CDS             3081..3746
                     /locus_tag="PLX22_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19043.1"
                     /translation="MVVLYIAVISVIVGVIVVLYSEFFEHTIMSNIPHDRYVPNLDVE
                     MSSHSMHNGKEQYHKTMDSDDIPDIDIDFDSIPEDETTIDTTPARSNPKSEVISIDEQ
                     SQQNNNKNDDVDVVMYVDSSGTIGDIGSGMRSENWSSLTRIGAGTLRYKQQGINIAIN
                     NKLYRYDFYRINNLKVYDNYMLFTLKGKQGVNCLLLNNESDILSFIENEYKQFVESHK
                     QDG"
     gene            3752..4747
                     /locus_tag="PLX22_03755"
     CDS             3752..4747
                     /locus_tag="PLX22_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19044.1"
                     /translation="MYYRYKSKQRKRNTTKLIINILLVAIVIYIVHEYHQYIFFWKYT
                     LNKLHYKVEMAMRQNDTIKRQGQLKDLCIAAKKYVESNPSSTDAYYLLGTVYYLYASS
                     ISNIPVYALYDENTLDTIPDEAKQNLINAIKAINKGLAFGGRSSIDDTTRIILASSLY
                     ASRFYPINEIYTIIQKVSNINKLSIDNARLYSLIAIKSGHTEEGLELVKLKGVSDNSE
                     GRMFLAASYLYANQQSNAIIEYRNILKNSMDNAIIKAVHIELGKIFYKKGIYTESLQH
                     LNAALQIDNQDIQCKLWLGKVYSAVGNIAQAKAVWSEVLLLDSENVEAKRLLGIM"
     gene            4876..5901
                     /locus_tag="PLX22_03760"
     CDS             4876..5901
                     /locus_tag="PLX22_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT19045.1"
                     /translation="MIFDSVYSLFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFIQKVHKRSTLV
                     RPRVVIGVPSGITEVEKRAVRESAEQAGAREIYLIEEALAAAIGANIPIHEPAGHMIV
                     DIGGGTTEIAVISLGGMVIADSVRIAGDEFDEAIIKYMRTQYNLVIGERMAEDVKFRV
                     GNAFPEKKIETMELKGRDAISGLPRTLEIDSTEIRKALKEPVDQILDAIKHTLERTPP
                     ELAADIVERGIVLSGGGSLLKGLDKYISKETGVPVIRAENPLTCVVMGAGKFLEEIKN
                     LYRSNLK"
     gene            5997..6866
                     /gene="mreC"
                     /locus_tag="PLX22_03765"
     CDS             5997..6866
                     /gene="mreC"
                     /locus_tag="PLX22_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT19046.1"
                     /translation="MEFFTRHKALILFIVFTLFCFISLSIQSSRVTNVVEGAGNMAIF
                     PFQKLYHAIQTNISLLWSGFSQLRDAQEELEKTRARLQYYESIAEEVEEIKRENQQLR
                     NLLGLKERLPYDSVLATIISKDPDNWFRTIIVNRGSSDGIKVNMPVVAFSGDEKAIVG
                     KIIEVRGSISRILPIISPDLKIGVVFQESRYPGLLSGYSAIGKMCIVDYVDKTATIKA
                     GDIVITSGQGGVFPQGLLVGVAIRSFPSESGAFQRVLVKPYINFDLIENVIIIKKEPD
                     LEILKLTEEETQQ"
     gene            6872..7363
                     /gene="mreD"
                     /locus_tag="PLX22_03770"
     CDS             6872..7363
                     /gene="mreD"
                     /locus_tag="PLX22_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOT19047.1"
                     /translation="MYIITGALVLASLIIQGHSSFDVLRIGGVKPDLVFIVIVYFAYS
                     FGSLYGEVTGFIGGILNDAISNSPLGFMAFPKMALGFIIGFFGRSIFKENLFTIFLLI
                     FFASIAKGVITLILAYLFHEGALSSVTSIILPESFYNALLSPPLFFLFDKIFEQELSR
                     EGR"
     gene            7367..>8821
                     /gene="mrdA"
                     /locus_tag="PLX22_03775"
     CDS             7367..>8821
                     /gene="mrdA"
                     /locus_tag="PLX22_03775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT19048.1"
                     /translation="MQFASVRTKIIEEFRKRLYVVGIIIICMFGIFFLQLINLQIIKG
                     REFSEKSRMNMESNIPLPASRGEIYDRNFSTDKQNIVLATNRPAFNIIVIPAQYKEKN
                     QLYYAIQNVCALLQLPYEEILTDLKSTNPYNRYIIQEDVPFDIVVTIATNQDKFPNIM
                     WEDAPVRVYPFNNMFSHVIGYIGSLTKEEYNTYRDVGYKYYQKIGKAGIERQYDSILR
                     GVDGYVRRIVDAKNRTEGEEIGQHPKSGYNIVLTLDYDVQKTAYTALGNNRGSVIVMK
                     PATGEIISIVSKPDYDPNLIISKNNIKIITELNNDKNKPFLNRAIQSRYPPASTFKLV
                     TSIAALEEEKWNPAITLYCPGKYTLKGYVDRDFYCYQAHGSVNLLWAIAKSCSVYFYQ
                     LGLKLGPTTIFNYAGYLGLAETSGIDIPGEITGFIPSKKWKLKTFGQPWFDGDTVNLS
                     IGQGFMLVTPIGMMDFLSAIVNSGVVFKPHCIKEI"
     assembly_gap    8822..8921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9041..10357
                     /gene="rodA"
                     /locus_tag="PLX22_03780"
     CDS             9041..10357
                     /gene="rodA"
                     /locus_tag="PLX22_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT19049.1"
                     /translation="MFKTIRSSFQEYDIKLLISIIALILIGIITIYATGFDPIEKVNS
                     GLYKKQLLWFIIGIICMFAISRVHYRILGDYSLYIYLVVLFFLIITTIFGAKIRNTRA
                     WINFGFFAIQPSEFMKLATILLLSKYLELRQFDIKRLRELFIPFAIVGVPVLLIYMQP
                     DFGTAMIFIPIFFALLFLGGADILQLISILGVATIALFVPMMLTYIEWSGINISGGFF
                     SFLKGNELLYIISGSLLAVSIISFILYFFFNSKVYRAIYIPSFIISAGLFLSVIIQKF
                     LKEYQKRRILVFLNPDLDPYGSGYNVIQSKIAIGSGGIIGKGFLKGSQSQLGFLPEKS
                     TDFIFSVISEEWGFIGAVIVLLLFGYILYKGCMIALKTSDKFGMLLAGGITCMIFFHV
                     FINIGMALGIMPVTGLPLTFLSYGGSNLIMSLLSIGILINIDSRTR"
     gene            10463..10918
                     /locus_tag="PLX22_03785"
     CDS             10463..10918
                     /locus_tag="PLX22_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19050.1"
                     /translation="MLIVLFIVLQISFSFHIYSLVLYVLRRENKYLKGFINTTISNIL
                     LAGGITALAIIHPQNVKEVDFKLLLWFMTGFIMLIMLFIKISIARAIFIRTKDPQNYH
                     FNYFGKKVLHSTVVRFEEILIFFFTMPFFLFCGAYFIARLFNLLLYKHL"
     gene            11045..11287
                     /locus_tag="PLX22_03790"
     CDS             11045..11287
                     /locus_tag="PLX22_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19051.1"
                     /translation="MGLITDKIRENAEKCKSCAFLKIRNAEIDKNSVLYDEAMMTLEG
                     VYRASCTMCPFEKDFEKIYGMKPYEYFPMRIAKKEA"
     gene            11304..11981
                     /gene="queC"
                     /locus_tag="PLX22_03795"
     CDS             11304..11981
                     /gene="queC"
                     /locus_tag="PLX22_03795"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOT19052.1"
                     /translation="MSKKKCVVLLSGGLDSATVAVFAKSRGFDIYALTFEYGQRHEIE
                     ILYAKKIAHYLNINAHDIVHLPDDIFKISSLVLSSGKEIPITRGSAIPSTYVPARNII
                     FLSIALAYAETVEATHIFIGVNAVDYSGYPDCRPQFIEAFQKMVMVGTKSGVEGNPII
                     IEAPLINMTKAEIIALGTSLGVDYSLTSSCYNPSPEGKPCGICDSCMLRKKGFEQAGY
                     EDPAIQR"
     gene            11956..12720
                     /locus_tag="PLX22_03800"
     CDS             11956..12720
                     /locus_tag="PLX22_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOT19053.1"
                     /translation="MKILPYNGKTAKNMKQSYINILLNTLMFDVLFPAFCSNCGKMIS
                     NINNGLCNNCYDNLNLSVYNYSSQYFFNVEEKIHGKRYWYIDDILLVFKYRNEVSKLI
                     YDMKFKNNKSIALLFSKFLLQGINSLAWDFDLITYIPVSKKRLWERGFNQSELMARHI
                     GKMLHKDVFNLMTIKNENNTQKKLSQNDRFISTYQKFDILSNCTNIVNKSVLIVDDVY
                     TTGATINECARILKMNSAKSVYASILAYAMLKDRDV"
     gene            12925..13251
                     /locus_tag="PLX22_03805"
     CDS             12925..13251
                     /locus_tag="PLX22_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19054.1"
                     /translation="MKIKRDDNLAILTLDGRIDQEMSEDLENALQKLIDDGCNNICMD
                     MTDVKHICSSALGVIVAFKRKIKNEGDIKLVITNENLLKLFQTTMLDKVFEIFESQRE
                     CLNAFD"
     gene            13273..14253
                     /locus_tag="PLX22_03810"
     CDS             13273..14253
                     /locus_tag="PLX22_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT19055.1"
                     /translation="MTPRKDIHDILKPIEHYLMAVDETVKSKLYTGIDLLDDSSMHTF
                     KKSGKKIRASMIILSGGLNNTIPDEIIDIACAAEIIHAASLVHDDIIDNADLRRGLPT
                     VSRKYGPKVAVLAGDYMYTKALEIAVGNNRTDLFPIMVDATVEMVKGELYQIQYSDID
                     NITIDNYYRIIEMKTARFMAACAKLGAAVAKMNTQQSDVLYECGLQLGYAFQITDDAM
                     DYLNAADITGKDAGNDFINGKITLPLLHLLEVADNKERALLKNYAKTPDNDNWNYVVS
                     KAKEYNVVDYCISIASDYVKKAIEIISMFPDNQYKNILTELALFFVDRKY"
     gene            14308..14529
                     /locus_tag="PLX22_03815"
     CDS             14308..14529
                     /locus_tag="PLX22_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19056.1"
                     /translation="MITKEKEEVLRLYDAGLEAYKKRKWDEAIDYFDKALKVDPNDGP
                     SKVYLERARQYKQTPPSDDWDGVFIMTTK"
     gene            14632..15054
                     /locus_tag="PLX22_03820"
     CDS             14632..15054
                     /locus_tag="PLX22_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT19057.1"
                     /translation="MAKKVIQVNKNPVYEIGMIATVFGKDTEFYGDLTFEKSLQINGY
                     FEGEISSGDFLVIGEKATVKANIKASTVIIRGTVYGNIEAKDKIEIQTEGKLFGNIRT
                     SKLIIADGVVFEGKCEMIKQPIAVQKSKQVHKESTLVE"
     gene            15096..16547
                     /locus_tag="PLX22_03825"
     CDS             15096..16547
                     /locus_tag="PLX22_03825"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOT19058.1"
                     /translation="MKEFVKKIDNCLYEIPEDYKKGMRVPARIYASYELLHHMDDAVF
                     EQLINVAMLPGIQKYALCMPDGHSGYGFPIGGVAAVDSDDGVISPGGIGFDINCGVRF
                     VITSVSIEEFAPKLNQIMDILFSRIPSGIGGKGIVSLSDNQFDEAMIKGARWAIENGY
                     GVKEDLLFCEENGCIGNANPDVVSGRARERGREQVGSLGSGNHYLEFQVIRNEDVLQT
                     TIAKKIGIIGNNQVSCMIHCGSRGFGHQIATDYLDKFIGVMKQKYHIDIPDRELACAP
                     FHSKEGQAYFGAMNCAINIAFLNRQLIYHVVRDTLCQVFKKSAEELGIALIYDVCHNT
                     AKLEQYQINGNNKKLLVHRKGATRAFTKGMKGIPDTYKEIGQPVLVGGSMQTASYILI
                     GGDRASDAFYSTVHGSGRIMSRQQAKKQFKGRELADSMAKSGIAIKTRSLSGLAEEAG
                     AAYKNIDDVIDTITAAGLSIPVARMIPVGSIKG"
     gene            16556..16996
                     /locus_tag="PLX22_03830"
     CDS             16556..16996
                     /locus_tag="PLX22_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOT19059.1"
                     /translation="MMESEEGFEFVENATLADIAIYCKAFTLEKLYAFNAEAFLHICL
                     ENPNDLNAKIDYRFSLQHQSLDLLLVKFLNELIFIKDTQGIVCKPVHVEIEYGQEGSM
                     CTAQLIGDYIINYRINVDPKAATLHKARCEKTNLIWESFIIIDV"
     gene            17017..>17358
                     /locus_tag="PLX22_03835"
     CDS             17017..>17358
                     /locus_tag="PLX22_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOT19060.1"
                     /translation="MAKIIDGKAIANEIKQEIKKDVAKLEQEYGKKPTLAVILVGNDS
                     ASEVYVRMKKKASEELGINSLQITLDERTTQSELLKLIHELNSDTNVNGILVQLPLPS
                     HIDENIIINTID"
BASE COUNT         5826 a         2393 c         3397 g         5642 t
ORIGIN      
        1 agatattgca gcccaatcct tgaaaaaggt gatcttgtta ttgttataag tcaatcaggt
       61 gaaacggctg ataccctggc agcgatgcgt gaagctaagg cacgtggtgt cagggtgctg
      121 ggcattacca atgttgtagg aagtaccata gctcgtgaaa gtgatggagg ggtgtacata
      181 catgcagggc ctgaaatagg tgttgcatct actaaagcgt ttacttcgca gataactgtt
      241 ttaatacttt taacatttta tttagcccgc cgcagattta tgactgctga tgcaggccgt
      301 gaattattac aggagttgat acaggtacct gcacttgtac aatcaatatt aggaaaaaat
      361 gatgaaatta aagaaatagc aacattatat caacatcacc gcaattttct ttatctggga
      421 agaggtatac agtttcctgt tgctcttgaa ggtgcattga agttaaaaga aatatcctat
      481 attcatgcag aaggatatcc tgctgctgag atgaaacatg gacctattgc acttatcgat
      541 gaaaatatgc cggttgtctt tattgctata aaagatgagg tgtatcaaaa agttataagc
      601 aatatgcagg aggtaaaagc acgaaaaggc aaaattattg ctattgccac cgagggtgat
      661 gaagagataa ataagtttgc cgatcatgtt ttctatgtgc cggctactgt aaaaattgca
      721 tccccacttt taacggtgat tccactacag ttgttagcat accacattgc ggtattgcgc
      781 gggtgcaatg tagatcagcc aagaaatctg gcaaagagtg ttacggtaga gtaaactgtt
      841 aaataaattt ttatgcactg tggtttaaat aattgacagg atgcaaacaa attttataag
      901 tgatagcagg aagtgtttat ggagtaatga gcatatatat ttaatgtgat gtgaaaatac
      961 agtaaaaaat tatgataata aggagaattc gaaatgatag taattgaaga tattgattat
     1021 attggtgtta cggtatccga catggataat gcaatcagtt tttacagtga tttgtttgac
     1081 tttgaggtaa ttgagcgtgt aagcaatacc gatgaagcat atatacgtgt tggtgatatt
     1141 ttattaaagc ttgttgaagt aaaagatttt aaagcgcccg aaaatttgaa aaataatatc
     1201 tgtttctata tcgatgaaga agattttgat gatgctctgg atgaattaca ggaaaaaaat
     1261 atcacaatag tttacggacc tgaaaatatc cggaaagggc gtctggttgt atttactgat
     1321 cctgatggaa ataaaattga attgcgatac ccccgatttt ctttataaaa ttaattaaaa
     1381 ttattaatca tgtcattaca gtttagcatt cctattatcc ccattggcgt tttttatgat
     1441 gttgccagaa cgctcaagat gtttgagctt ttaatgcaac acggtatacg ttgtattgaa
     1501 gttacattac gaacggcgca ggcgcttgat tgcattactg ctctaaaaaa tacatttcct
     1561 gatgcaatga ttggtgctgg cagtgtcata actcatgacg attttaaggc agcagtggat
     1621 gctggagcaa tgtttattgt ttcaccatgt gtaagtgatt ctttaataga actatcgcac
     1681 aaaaataaaa atatatatta tatacctggt tttacaactc ctaccgaatt agctcatgca
     1741 ctgcaaatgc acgttgatgt tttgaaattt tttcctgctg agtattgtgg tggcgtgcac
     1801 tacctcaaag ctattgttga accgtttgct caattttcat tttcaatcat tccaacgggt
     1861 ggtattaaac ctgctcatat tgctgagtat ttatcgttgc caaaggtagt tgcatgtggt
     1921 ttgtcatata ttgtcgatag ttacctgata gaacatggcg attatataaa tctggaaaaa
     1981 cgtataaacg aaatccttgc ctgtttgcag cgatagatgt ccatctataa aaacattgaa
     2041 acagcaattt ataaacggga aaacctgttg aagcaaacta tcaccaatgc gttccgtctg
     2101 gttaatggac agggtgatgg ttgctatgga ctgatacttg attactatga tgattatctt
     2161 ttattacagg tgtacgatag ttccctgtat caggaatatc ataggataag cgatcattgt
     2221 ataaaaatat tggcatgttg caatagagca ataaagggtg ttctgttaaa agataggagt
     2281 aaagttaatg atcctcaaaa aattgcagaa tcacgaaaaa gcgtgtgtat aacaggaaca
     2341 ctgccaccgg tacctttgat agcaaaacaa aatgacattg ttgcaggtgt tgatctgatt
     2401 aatggccaga atacagggct ttttcttgat atgcgcggag tgcgacagcg tttagcacca
     2461 tattatgcaa acggtggaaa gctctgtaat ctttttagct ataccggtat atttgcgatc
     2521 catgcattga aaaatggatt acaccatgca gtcaatgttg atatgtcgaa aagcgtgtta
     2581 tacagggcta aacaaaatta tatgctcaat cacatcaagg atgataatag agactttgtt
     2641 gctatggatt gcaaaaaatt tttacaatat gctgcaaaac ataatgatac atttcatttt
     2701 gtgatttttg atccacctac attttccaga aataaaggac acaatttcag tgtaaaaaaa
     2761 cattataacg aatttttatc gttgattgct cccattgttt cagatgggta catccttacc
     2821 actatcaata ccatttcaat ttcagatgat gagtatatat cattacatcc cggtaactgg
     2881 gaactgcaat ttttagaaca tgagcctgat gacttcccct attataagga accctacctg
     2941 aaagcaggtt tatggaaaat gcggtaattt ttttgaatta atccttgact tgtaaaattt
     3001 atctttcgac aataatgatg ggtggttttc tttattaatc attcatgttg caattccagt
     3061 gaactataca ggatagtatt atggttgttt tatacattgc ggtaatcagt gtcatagtgg
     3121 gtgtcatagt tgtactttat tcggaatttt ttgagcatac cattatgtcc aatatccctc
     3181 atgaccgata tgtgcctaat cttgatgttg aaatgtcatc acactccatg cacaatggca
     3241 aggagcaata ccataaaaca atggattctg atgacatacc tgacattgat atagactttg
     3301 attctatacc agaagatgaa acaacgattg atactacacc tgcaaggagc aatccaaaat
     3361 cagaagttat ttctatagat gaacaatcac agcaaaataa caacaagaat gatgatgttg
     3421 atgttgttat gtatgtcgat agtagcggaa ctattgggga tattggaagc ggtatgcgtt
     3481 cagaaaattg gagttcatta acaaggatag gagccgggac tttgcgatat aaacagcagg
     3541 gcatcaatat tgctattaat aataaactct atcgctatga cttctataga ataaataatt
     3601 taaaagtata tgataactat atgcttttta cgttaaaagg taaacagggt gttaattgtt
     3661 tgcttctcaa caatgaatcc gatattttat catttattga aaatgagtat aaacagtttg
     3721 ttgaatctca caaacaggat ggttaacaag tatgtattat cgatacaaat caaaacagcg
     3781 aaaaagaaat acaacaaaat taattattaa tatcctttta gtggcgatag ttatttatat
     3841 tgtgcatgaa taccatcaat acatcttttt ctggaaatac acattaaata aattgcatta
     3901 taaagttgag atggcaatga gacaaaatga tactataaag cgacagggac aattgaaaga
     3961 tttatgtata gctgcaaaaa agtatgttga gagtaatcct tcaagtactg atgcttatta
     4021 tctgttggga acggtttatt atttatatgc atcctctatc agcaatattc ctgtatatgc
     4081 attatatgat gaaaatactc tggatacaat ccctgatgaa gcaaaacaaa atttgatcaa
     4141 tgctattaaa gcaattaata aaggattagc ttttggtggc agatcatcga tagatgatac
     4201 tacaaggatt attcttgcct catcacttta tgcaagcagg ttttatccta ttaatgagat
     4261 atatactatt attcaaaaag tatcaaatat aaataaacta tcgatagaca atgctcgatt
     4321 gtattcattg atagctatca aaagtggtca tactgaagaa gggcttgaac ttgttaaatt
     4381 aaaaggtgtc tctgacaata gtgaaggaag gatgttttta gctgcatcat acttatatgc
     4441 taatcagcag agcaatgcta ttattgagta caggaatatt ttgaaaaatt caatggataa
     4501 cgccattatt aaagctgtac atatagaatt agggaagata ttttataaaa aaggcatata
     4561 tacagaatca ctgcaacatc tcaatgctgc attgcagata gataatcaag atatacagtg
     4621 taaactgtgg ttaggtaaag tttattctgc agtaggaaat attgctcagg caaaagcagt
     4681 gtggagcgaa gtattgctcc ttgattctga gaatgttgaa gcaaaacggt tattgggaat
     4741 tatgtaaaaa ttcatttttt atctgctatt aaaaaaattc atgataatag cttgacaaaa
     4801 aaaatagtaa agtatcattc aaatgcaaat agttttactg ttaatcaata ttttgactaa
     4861 aaaggtaaac atattatgat ctttgattct gtttatagct tattttctaa tgatatgggg
     4921 attgatttag gtactgctaa tacattggtt catgtaaaag ggcagggtat tgtattaagt
     4981 gaaccatcgg ttgtggctgt tcaaacaagt accggtaaag tgctggcagt tggccatgag
     5041 gcaaaaagaa tgctgggaag aactcctggc gatattgtag caatacgtcc tatgaaggat
     5101 ggcgtcatag ctgattttga aactgttgag aaaatgatac gatattttat ccagaaagtt
     5161 cacaaacgat caacattggt tagaccacga gtggtaattg gtgttccctc tggtatcacc
     5221 gaagtggaaa agcgtgcggt acgagagtcg gcggagcagg caggtgcaag ggaaatatat
     5281 ctcatagaag aagcattggc ggctgccatt ggcgcaaata taccaataca tgagcccgca
     5341 ggacatatga tcgttgatat tggaggtggg acaactgaaa tagcagttat ttcattgggt
     5401 ggtatggtta ttgctgattc cgtaaggatt gcaggtgatg aatttgatga agccatcata
     5461 aaatatatga ggacacagta taaccttgta attggtgaac gcatggctga ggatgttaag
     5521 ttcagggttg gtaatgcatt cccggaaaag aagattgaaa caatggaatt gaaagggcgt
     5581 gacgcaatat caggtttgcc cagaacactg gaaattgatt ctactgagat tagaaaagcg
     5641 ctcaaagagc cagttgacca gattctggat gctatcaaac atacattgga acgaacccct
     5701 cctgaattag ctgctgacat agttgaacgt ggtatagtat tatccggtgg cggctcactg
     5761 ctaaaaggtc tggataaata cattagtaaa gaaacgggag ttcctgttat aagggctgaa
     5821 aatccactta cctgtgttgt gatgggcgct gggaaattcc ttgaagagat taaaaatctc
     5881 tatagatcaa atttgaaata aattataata gtgtaattta ttatcgtaat tatcattaaa
     5941 aatgctcttg cagattatgc aggagtattt atttaataaa ataaattagg taccatgtgg
     6001 aattctttac acgacataaa gcgttgatac tttttatagt atttacatta ttttgcttta
     6061 tttccctctc tattcaatcg tcacgagtaa ccaatgtggt tgaaggggcg ggaaatatgg
     6121 caatttttcc atttcagaaa ttatatcatg caattcagac caatatttca ctattatggt
     6181 caggattttc ccaattgagg gatgcacagg aagaattgga aaaaacacgg gcacggttgc
     6241 aatattatga atcgattgct gaagaagttg aagaaataaa aagagaaaat caacaattaa
     6301 gaaatctcct gggtcttaaa gaacgattac cgtatgactc ggttttagct accataatat
     6361 caaaagatcc tgataattgg tttagaacaa tcattgtaaa tagaggaagc tctgatggta
     6421 ttaaggtaaa tatgcctgtt gtagcttttt ctggtgatga gaaagctata gttgggaaga
     6481 taatagaagt aagaggttca atctcaagaa tattgccaat tatttcaccc gatttaaaga
     6541 ttggagttgt ttttcaagaa agtcgttacc ccggactttt gagtggttat tctgcaatag
     6601 gtaaaatgtg catcgtagat tatgtggata aaacagcaac tattaaggca ggggatattg
     6661 tcattacctc cggtcaggga ggtgtattcc cgcaaggact attagtggga gtggcaatac
     6721 gatcattccc ttctgaatca ggtgccttcc agcgtgtact tgtaaaacca tatattaatt
     6781 ttgaccttat tgaaaatgtt ataattataa aaaaagaacc ggacctcgaa attttaaaac
     6841 taactgagga agaaacacaa caatgatatt aatgtatatt ataacaggag cattagtatt
     6901 agcatcattg ataatacagg ggcattcttc atttgatgtt ttacgtatag gaggtgttaa
     6961 acctgatttg gtttttattg tcattgtgta ttttgcctat agttttggtt ctttatatgg
     7021 tgaggtaacc ggatttattg gtggtatact aaatgatgct atatccaatt ctcctctggg
     7081 atttatggca tttccaaaaa tggcattagg gtttataatt ggcttttttg ggaggtcgat
     7141 ctttaaagaa aatttattta caatatttct attgatcttt tttgcttcta ttgccaaagg
     7201 agtaataact ttaatattag cctatctttt tcatgaaggg gcattgtcat ctgttacatc
     7261 aataatttta cccgagtcgt tttataatgc attactatct cctccattat tttttttatt
     7321 tgataaaatt tttgagcagg agttgtccag ggaaggccga tagattatgc aatttgcatc
     7381 cgttagaaca aaaatcatag aagaatttag aaagcgctta tatgttgtag gtattattat
     7441 tatctgcatg tttggaatat tttttttgca acttattaat ttacagatta ttaagggcag
     7501 ggaattttca gaaaagtcaa gaatgaatat ggaaagtaat attccattgc ctgcttcaag
     7561 aggagaaatt tacgatagaa atttttctac tgataaacag aacattgttt tggcaacaaa
     7621 tcgccctgct tttaatatta ttgtaatccc tgctcagtat aaagaaaaaa atcaattata
     7681 ttatgctatc cagaatgtat gtgctctttt acaattgcca tatgaagaaa tcctgacaga
     7741 tttaaagtca actaatccct acaacagata tatcattcag gaagatgtcc cgtttgatat
     7801 tgtagtaact atcgccacaa atcaggataa atttcctaac ataatgtggg aagatgcacc
     7861 ggtgagggta tatccattca ataatatgtt ttcgcacgta ataggttata ttggaagttt
     7921 aacaaaagaa gaatacaata catacaggga tgttggatac aaatactatc aaaaaattgg
     7981 aaaagcaggt attgaacggc agtatgattc aattttgcga ggtgttgatg gatatgtccg
     8041 tagaatagtc gatgccaaaa atagaaccga aggtgaagaa attggacagc atccaaagtc
     8101 aggttataat attgttttaa ccctcgatta tgatgtacag aaaactgcat atacagcact
     8161 ggggaataac aggggttcag ttattgttat gaaaccggct accggtgaaa taataagtat
     8221 agttagcaaa ccggattacg atccaaatct tatcatctca aaaaataata taaagataat
     8281 cacagaatta aataatgata aaaataaacc ttttctgaac agggctattc aatcgcgata
     8341 tcctcctgca tcaacattta aattagtaac ttctattgca gctctggagg aagaaaagtg
     8401 gaatcccgca attactctgt actgcccggg aaagtatact ttaaagggat atgttgacag
     8461 agacttttat tgttatcaag cacatggatc agtaaattta ttatgggcta tagcaaaatc
     8521 atgcagtgtt tatttttatc aattaggatt aaaattaggg ccaacaacca tatttaatta
     8581 tgcaggttat ttaggtcttg cagaaacttc aggtatagat ataccgggtg aaatcaccgg
     8641 atttatacca tcaaaaaaat ggaaattgaa aacttttggg cagccatggt ttgatggtga
     8701 taccgttaat ctgtccattg gtcagggatt tatgctggta actcctatcg gcatgatgga
     8761 ttttttatca gcaattgtta attcgggtgt tgttttcaaa ccacattgta ttaaagaaat
     8821 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatccggaa aaggtggtgg
     8941 cgatagttgt tatggtagaa tatggtattg ctggtgctgc tacagccgtg cctatagctg
     9001 aacaggtttt tacaactctg attaagaaag gatactttta atgtttaaaa ccatacgatc
     9061 ttcttttcag gaatatgata taaagctttt aatttcaatc atagctttga ttttgatagg
     9121 cataataacg atttatgcta caggttttga cccaattgag aaggtaaata gtggtttata
     9181 taaaaaacag cttttatggt ttataatagg cattatctgt atgtttgcaa tttcaagagt
     9241 tcactatagg attttaggtg attattctct ttatatttat ctggtggttc tatttttcct
     9301 tataattaca acaatttttg gtgcaaaaat acgtaataca cgtgcgtgga ttaattttgg
     9361 tttttttgcc attcaaccat ctgaatttat gaaattagcc actattttgt tattatcaaa
     9421 atatcttgaa ttacgacaat ttgacattaa gcgtttgcgt gaattgttta taccttttgc
     9481 aattgttgga gttccggtgc tgcttattta tatgcagcct gattttggaa ctgcaatgat
     9541 atttattccc attttttttg cattactctt tttaggtgga gcagatatac ttcagcttat
     9601 atcaatattg ggtgtagcaa ctatagcatt atttgttccc atgatgctta cctatattga
     9661 atggagtggc attaatattt caggaggttt tttttcattt ttaaaaggga atgaattatt
     9721 atatattata tctggttctt tattagcggt ttctattata agtttcattc tttatttctt
     9781 ttttaattca aaagtatata gggctatcta tataccctca tttattatat cagcagggtt
     9841 atttctatca gtaattattc aaaaattttt aaaagaatat caaaaaaggc gaatccttgt
     9901 ttttcttaat cctgatcttg atccctatgg atcaggatac aatgttatac aatctaaaat
     9961 tgctattgga tcaggtggca ttattggtaa aggtttttta aaaggatctc agtcgcaatt
    10021 aggcttttta ccagaaaaat ctactgattt tatcttctca gtaatttctg aagaatgggg
    10081 ttttattgga gcggttattg tacttttatt atttgggtat atactttata aaggatgtat
    10141 gattgcttta aaaacttcgg ataaatttgg gatgttactt gccggaggta taacatgtat
    10201 gatatttttt catgtattta ttaatattgg tatggcttta ggtataatgc cagtaacagg
    10261 tttaccttta acctttcttt catatggtgg atcaaacttg attatgtcat tgttgagtat
    10321 tggtattctt attaatattg attcacgcac aagataatat tttattatat aaaaaataaa
    10381 ataaagaatg cttgcaaata atttgctttc atgtatacta taatgggtat gtttttttga
    10441 tttcgattat ataggaggac aaatgttaat tgtccttttt atagtattgc aaatttcttt
    10501 ttctttccat atatattcgc ttgtattata tgtcttacga agagaaaata aatatttaaa
    10561 aggcttcatt aatacaacta tcagcaatat attgttagcc ggtggaataa ctgctttagc
    10621 tataatacat ccacaaaatg tcaaagaggt tgactttaag cttctgctgt ggtttatgac
    10681 aggatttata atgctcatta tgttatttat taaaataagt attgcacgag cgatttttat
    10741 acgtacaaaa gatccgcaaa attatcattt taattatttt ggtaaaaaag tattacattc
    10801 tactgtggta agatttgaag aaattttaat attctttttt acaatgcctt tctttttatt
    10861 ttgtggtgct tattttattg caagattgtt taatctgtta ttatataaac atttataaaa
    10921 atattattaa aataatttat atattgttca tgttatataa aaatatgctg gattttttat
    10981 tgctattttc atattattgt atacagtatt ataatatcta caatcaatga cgggaggtgt
    11041 tttaatgggt ttaattaccg ataaaatacg tgaaaatgct gagaaatgta aatcctgtgc
    11101 atttctaaaa ataaggaatg cagaaattga taaaaatagc gttctttatg atgaagctat
    11161 gatgacatta gaaggggttt atagggctag ttgtacgatg tgcccttttg agaaagattt
    11221 tgagaagatt tatggcatga aaccctatga atattttcca atgcggatag caaaaaagga
    11281 agcataataa gtttaattac acaatgagta aaaagaaatg tgtagtgttg ctcagtggcg
    11341 gattagattc tgccactgtt gctgtatttg caaaaagcag gggatttgat atttatgccc
    11401 ttacctttga atatgggcaa cgtcacgaga ttgaaattct ttatgcaaaa aagatagccc
    11461 actatctgaa tattaatgct catgatattg tgcatttacc ggatgacata tttaaaatat
    11521 catctctggt gctatcatct ggcaaggaaa taccaatcac ccgtggaagt gctatcccat
    11581 caacctatgt cccggcacga aatattatat ttctttcgat tgcattagct tatgcagaaa
    11641 cagtagaggc tactcatatc tttattggtg tgaatgcggt agattatagt ggttatccag
    11701 attgcagacc tcaatttata gaagcattcc agaaaatggt aatggtgggt acaaagagtg
    11761 gggttgaggg aaatccaatt attattgaag caccacttat caatatgact aaagcagaaa
    11821 ttattgcatt aggtacatca ttaggtgttg attattctct gacatcaagt tgctataatc
    11881 catcacctga agggaaacca tgcggtatat gcgatagttg tatgctaaga aaaaagggat
    11941 ttgaacaggc agggtatgaa gatcctgcca tacaacggta aaactgcaaa aaatatgaaa
    12001 cagagttata ttaatatact tttaaatact ttaatgtttg atgtactatt tcctgcattt
    12061 tgttctaatt gtgggaagat gatatcaaat atcaataatg gtttatgcaa caattgttat
    12121 gataatctta atttgagtgt ttataattac agcagtcaat atttttttaa tgttgaagaa
    12181 aagattcatg gtaaacgtta ctggtatatt gatgatatat tattagtttt taaatataga
    12241 aacgaagtat caaaactgat atatgatatg aaatttaaaa acaataaatc tattgcttta
    12301 ttgttttcaa aatttctgtt acaggggatc aattctttag catgggattt tgatcttata
    12361 acatatattc cggtttctaa aaaaaggtta tgggaacgag ggtttaatca atctgaactt
    12421 atggcacggc atattggtaa aatgctacat aaagatgttt tcaatttaat gacaattaaa
    12481 aatgaaaaca atacacaaaa gaaattaagc caaaatgata ggtttatcag tacatatcaa
    12541 aaatttgata ttttaagtaa ttgcactaat attgtaaaca aatcagtttt aatagttgat
    12601 gatgtttata ccactggtgc aactattaat gaatgcgcgc gtattttaaa gatgaatagt
    12661 gctaaaagtg tttatgcatc tatactggca tatgcaatgt tgaaagatag ggatgtttaa
    12721 tgttatttat aataatacat ttaacagtgt ttgaagtgac tactactgta attattttat
    12781 ataaatatct tggaaagttt tgaacactac agcaattgta attgagcatt agagtgacta
    12841 aaataatggt tgtaattaca ttcgcgatag gttaaaacaa atagtatatc tacttcagga
    12901 ttaacaaaga ataagaggtg aaatatgaaa attaaaaggg atgataatct ggctattctt
    12961 actttagatg gaagaattga tcaggagatg tcagaagatc ttgaaaatgc attacagaaa
    13021 ttaattgatg atggttgtaa taatatttgt atggatatga ctgatgttaa gcatatatgt
    13081 agttcggcat taggtgtgat agtagctttt aaaagaaaga taaaaaatga aggtgatata
    13141 aaattagtaa taactaatga aaatcttctt aaactttttc aaaccaccat gcttgataaa
    13201 gtatttgaaa tatttgaatc acaacgtgaa tgtttaaatg cttttgatta attgttgata
    13261 aaagagatat atatgacgcc acgaaaagac atacacgaca tactaaagcc tattgaacat
    13321 tatttaatgg ctgtggatga aacagtaaaa agcaaattat atacaggcat tgatttgctg
    13381 gatgatagca gcatgcatac gttcaaaaag agcggtaaga agataagggc ttctatgatt
    13441 attttatccg gtggattaaa caatactata cctgacgaaa ttattgacat agcctgtgct
    13501 gctgaaatta ttcatgctgc atcgttagtt catgatgata ttattgataa tgccgattta
    13561 cgaagaggct tgccaacagt ttcaaggaaa tatggtccca aagttgcagt gcttgctggt
    13621 gattacatgt acacaaaagc gctggaaata gctgttggca acaatagaac agatctcttc
    13681 ccgataatgg ttgatgcaac tgttgaaatg gttaaagggg agctatatca aattcagtat
    13741 tctgatattg ataatataac aattgataat tactatcgca taattgagat gaaaactgca
    13801 cgttttatgg cagcatgcgc aaaattaggt gccgctgttg ccaaaatgaa tacacaacaa
    13861 tctgatgtat tatatgaatg cggattgcag ttgggatatg cttttcagat aaccgatgat
    13921 gctatggatt atctcaatgc cgcggatatt accggtaaag atgctggcaa tgattttatt
    13981 aatggcaaga ttaccttgcc tttgttgcac cttttagagg tagcagataa taaagagcgt
    14041 gcactgttaa aaaattatgc aaaaacccct gataacgata attggaatta tgtagtatca
    14101 aaggcaaaag aatataatgt ggttgattat tgtataagta tagcatcaga ttatgtcaaa
    14161 aaagctattg aaattatctc tatgtttcca gacaaccaat ataaaaatat tttaaccgaa
    14221 ttagcattgt tctttgttga cagaaaatat tgatataggt ataatttatt attaaactta
    14281 gataaaatat tgtaaaggaa atagtatatg ataacaaaag aaaaagagga agttttacga
    14341 ttatatgatg ctggattgga ggcttataaa aagcgtaaat gggatgaggc tattgattac
    14401 tttgataagg cacttaaagt tgatcccaat gatggtcctt ctaaagtata tcttgaacga
    14461 gcaagacaat ataaacaaac acctccctct gatgattggg atggcgtctt tataatgact
    14521 accaaataga ttgtttttta aaatttggct tgtattaaaa aaatcatgta tcaacattgc
    14581 tgttataata atatataata aatatttgtt taatagaaga aggtatatac aatggctaaa
    14641 aaggttatac aggttaataa gaatccagta tatgaaattg gaatgatagc aactgttttt
    14701 ggtaaagata cagagtttta tggtgatctt acctttgaaa aatctcttca aattaatggt
    14761 tattttgaag gtgaaatatc ctcaggtgat tttctggtga ttggtgaaaa ggctaccgtt
    14821 aaagccaata taaaagccag tacggttatt atcagaggaa ctgtttatgg taatattgaa
    14881 gctaaagata agattgaaat acaaactgaa ggtaaacttt ttggaaatat ccgtacatca
    14941 aaacttatca ttgcagatgg cgtagtattt gaaggaaaat gtgaaatgat taaacagccc
    15001 atagcagttc aaaaatcaaa acaagtccac aaagaaagta ctctggttga atagatagta
    15061 taatctgttg gcaatattta tataattaca attaaatgaa agaatttgtt aaaaaaattg
    15121 acaattgttt gtatgaaatt cctgaagatt ataaaaaagg aatgcgtgtg ccagcacgca
    15181 tttatgcatc gtatgagctt ttgcatcata tggatgatgc agtatttgaa cagcttatca
    15241 atgttgccat gctcccgggt atacaaaagt atgcattatg tatgcctgat ggacactctg
    15301 gatatggatt tcctataggt ggtgttgcag cggttgatag tgatgatggt gttatttcgc
    15361 ccggtgggat agggtttgat attaactgcg gtgtacgttt tgttataacc tcagtgagca
    15421 ttgaagagtt tgcacctaag ctgaaccaga taatggatat tttattttca cgcattccca
    15481 gtggtattgg tgggaagggt attgtttcat tatccgataa ccaatttgat gaagcaatga
    15541 taaaaggagc cagatgggca atagagaatg gttatggtgt aaaggaggat ttattatttt
    15601 gtgaggaaaa cggatgtatt ggcaatgcca atccagatgt tgtttccggg agagcacgtg
    15661 aacgtggaag ggagcaggtg ggttctttgg gttcgggaaa tcattatctt gaatttcagg
    15721 ttattcgcaa tgaagatgtt ttacaaacaa ctatcgccaa aaaaataggc attattggga
    15781 ataatcaggt cagttgcatg atacattgtg gaagcagagg ttttggtcat caaatagcaa
    15841 cagattattt agataagttt attggggtga tgaagcaaaa gtaccatata gatattcctg
    15901 atagggaact ggcgtgcgca ccgtttcatt ctaaagaagg acaggcatat tttggggcta
    15961 tgaattgtgc gataaatatt gcatttttaa atcgccagct tatatatcat gttgtacgtg
    16021 ataccctatg ccaggttttt aaaaaatcag cagaagaatt aggcatagca ttgatctatg
    16081 atgtttgcca taacacagca aaattagagc aataccagat taatggtaac aataaaaagt
    16141 tactggtaca caggaaaggt gcaacccgtg cttttacgaa aggtatgaaa gggatacctg
    16201 atacctataa agaaattgga cagcctgttc ttgtaggtgg gagcatgcaa acagcttcat
    16261 atatattaat tggtggagac agggcttccg atgcatttta cagcactgtt catggcagtg
    16321 gtagaatcat gtcacgacag caagcaaaaa agcaatttaa gggtagggag cttgcagata
    16381 gcatggcgaa aagcggtatt gccattaaaa cacgttcatt gagtggttta gctgaagaag
    16441 ctggagcggc gtataaaaac attgatgatg ttattgacac tattacagca gcagggttaa
    16501 gtattcctgt agcacgaatg ataccagtag gctccatcaa agggtagttg tgaacatgat
    16561 ggaaagtgaa gaaggttttg aatttgttga aaatgcaact ttagcagata tagcaattta
    16621 ttgtaaggct tttaccttag aaaaacttta tgctttcaat gctgaagctt ttcttcatat
    16681 atgtctggaa aatccaaatg acttaaatgc taagattgat tacaggtttt ctttacaaca
    16741 tcaatctctt gacctgttgc tggtaaaatt tttaaatgaa ttaattttta ttaaggatac
    16801 acagggtata gtatgtaaac cagtacatgt tgaaatagag tatgggcagg aaggtagtat
    16861 gtgtacagcg caattgatag gtgattatat aattaattac cggattaatg tagatcctaa
    16921 agctgcaaca ttacataaag ctcgttgtga aaaaacaaac ttaatatggg aatcatttat
    16981 aattattgat gtatgataag aaaggaaggt tacacaatgg caaaaataat agatgggaaa
    17041 gcaatagcta atgaaataaa acaggagata aaaaaggatg ttgcaaaact ggaacaagaa
    17101 tatgggaaaa aaccaacact ggctgtgatt ttagttggga atgatagtgc atctgaagta
    17161 tacgtgcgca tgaaaaagaa agcatctgaa gaacttggca taaactctct tcagattaca
    17221 ttagatgaaa gaacaacaca atctgaattg ctaaaattga tacatgagct gaatagtgat
    17281 accaatgtca atggtattct tgtgcaactg ccattaccat cgcatattga tgaaaatata
    17341 attattaata ccatcgat
//
LOCUS       DAOSOX010000030        17134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3359_length_17134_cov_8.199719, whole genome shotgun sequence.
ACCESSION   DAOSOX010000030 DAOSOX010000000
VERSION     DAOSOX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3359_length_17134_cov_8.199719"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLX22_03840"
     CDS             complement(<1..1520)
                     /locus_tag="PLX22_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19061.1"
                     /translation="MDDVVRILEDIINDFDVNKFKNFFYKKTNDFISQDKPLDGCNDE
                     LFDESTQIGTFKVNDVHEFIVIAAKVNKELTQKSGKKAQYTFAKKILKQMIQYTGGFF
                     IFYDSNGNFRFSFIYSIPRPDGKQDWSNFRRYTYYVSKDQTNRTFLQQMQAASFESLE
                     KIIDAFSVEKVTKEFYRNIVELYYTLINNYNNKGLQRFPKEDQNEKEEFAIRLLGRLL
                     FCWFLKMKKDGSKDSLIPQKYLSSELVKNFNGDYYHEYVEPLFFQILNKPLKERLEKY
                     KTKSYSLIPFLNGGLFEPHDSDYYPVTAINEMGISPNINILTISNDWFANLFEVFERY
                     NFTIDENTLVDTEVSIDPEMLGKIFENLLAEYNPETKENARKATGSYYTPREIVDYMV
                     DESLKNYLCEKTKLSRDIIEELLKHENFEAAANLNEHDKEAIIDAIDKIKVIDPACGS
                     GAFPMGILHKLQVILENVDPQSKIWFKKKIGQIVDPLLQKELQIKLEEENWDYIHK"
     gene            complement(1548..4925)
                     /locus_tag="PLX22_03845"
     CDS             complement(1548..4925)
                     /locus_tag="PLX22_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT19062.1"
                     /translation="MSNFITNQPTKNLLHRVRDLIAHSKELKFLVGFFYFSGIKDLYT
                     SLQNNADVVVKICVGLNVDTLNYCIVECGNDANVSNKDVINRYFESITKSINSEEFDN
                     KDFYEQIAYFIQLIKNERLIIRKTRQPNHAKLYLFTLSTEQVKQKILITGSSNLTRAG
                     LIGQHEFNVEITDYGIEEAERYFDTIWDESVAITENDACKKELINLLQHQTLIKEITP
                     FEAYCLVLKSYLDTYEIRTASEAINRILISNNYVPYRYQLDAITQAIAIIEKHNGVIL
                     ADVVGLGKTIIACAIAFQLKKRGIVICPPGLIGNDNKDMGWKKYLEDFRLFDWEVRSS
                     GKLEEALEFVNRCKDIEVVIVDEAHRFRNQDTRSYELLKNICRDKIVILLTATPFNNR
                     PSDIFSLIKLFVTPRKSTITLENNLELLFSMYKSKFDKLSYIKRYHDSKDREKRNRAT
                     TFYKYLFGTENIDIRRVNAETHKLAKQIRDVIEPVTIRRNRIDLQKNPYYKDEVQHLS
                     IVKDPIEWFYELTPKQLQFYDTIIEHYFVEDDVEAFKGSIYRPFIYEVGELEINDDND
                     VNEGLLEKNREYQQQKNLFNFMRRLLVKRFESSFGAFKESIKNFKRIHEHVLQFIEKT
                     GGGDPLKGEFILDRQLLEKIYDMDVDEIEQYLQEYETNLQKDVLPKKYKRYKIKTFKY
                     KEKFIDDIKHDIKLFTSILEQLDALDLVNNDPKLDCLIKNVANEFYQNKEKDKNKRKI
                     VIFSEFLDTVCYLKDTLNNKFNNRVLVISGDLSDSKIKEIYKNFDASYQDQKDDYDIL
                     LCTDKLSEGFNLNRAGMIVNYDIPWNPVRVIQRLGRINRISKKVFDELYIVNFFPTEK
                     GAEIVKSREIAQNKMFMIHNTLGEDAKIFDEDEEPTPAELYHRLQINPETLEAESFYT
                     RVLNVFESIKAQYPEIIKNIESTPHRVKVSKKSTYDELLVFFKKNRLFVMNYDYSDGS
                     IVETTLEEVVDRIACNIDEKALPIDERFWGAYEKVKVHKDKSSLPKSVLSIQDRAKNK
                     LQFILSNECPEALQPYIPFLKQLKEDLIDYGTLSDYTLRRIANFNIDYNDATKLQELV
                     NEIAALQQELGNDYLHKIKAKEDGLQKEIIIAIENKI"
     gene            complement(5066..5740)
                     /locus_tag="PLX22_03850"
     CDS             complement(5066..5740)
                     /locus_tag="PLX22_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19063.1"
                     /translation="MKAKSMVIMLLFFAIGCAHYEVMVSSINNNETAGNKVFVAPGMK
                     DVAINDLQFQEFSNYIKKVLIKKGFAIVNTIDEADQVVMLVYAISGPQNYTANVPLWG
                     PTGIESSTTYGNISSSGSYYGNTYYNQTYGITGVAQVKRTFYSRTIILIAFDWKLFKT
                     KKVEKQLWKTDIVSIGSSNDLRYVFPYMVVAAEQYIGTNTRHAVTVKINEYDRRVKEY
                     IGYDDK"
     gene            complement(5818..6774)
                     /locus_tag="PLX22_03855"
     CDS             complement(5818..6774)
                     /locus_tag="PLX22_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT19064.1"
                     /translation="MSQNERLQYIDTSIREKGVVTVKEVVEKFEVSPRTVKRDLEYFR
                     DRINAPIVYDVYKKGYLYSLPFDYFSYRNERMLLFYLFLMKLIESPTHFPFVDKAIPD
                     LIKRNILAHYLPLMSRITYEIDEWEQLDITLFSKLFSTLRNDNCIVIKYADVTGKTTT
                     RTIAPEHFIHYEGRWYVIAYDETKKGIRTFLLSRIQKAEVVNKKRTRKINDDELQKFI
                     KSTYGIYKGSGGKLVQVRFYQPVCNIVKKQVWHKEQTMKEGKHEQYGAYVELQLPVHH
                     PQELLGKVLRYGKYAEIVAPSSIRTMWLEAIKEAYVRFVKSK"
     gene            7231..8910
                     /gene="ilvB"
                     /locus_tag="PLX22_03860"
     CDS             7231..8910
                     /gene="ilvB"
                     /locus_tag="PLX22_03860"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOT19065.1"
                     /translation="MKITGAQIIIESLIREKVEVMFGYPGGVVIPIFHHLFNAPFKFI
                     LSRHEQGAIHAADGYARATGKPGVVIATSGPGATNLVTGIATANMDSIPLVIFTGQVS
                     TEVIGNDAFQEADVTGITRPITKHNYLVKDINELAKIIKEAFYIATTGRPGPVLIDVP
                     VNISNAETEFHYPETVHLRSYKPVIHGHSGQIQKACKLIEQSKRPVVYAGGGVISSNA
                     SDELRTFIKKTGIPITTTLLGLGAYPENDKLSLKMLGMHGTYYANHAVHQSDLLIAVG
                     ARFDDRVTGKISEFIPHAKVIHIDIDPSSVSKNVAVDVPIVGDAKHVLTEINKTVKAP
                     KIDEWVTHVMKMKEENPLTYKDSDVTIKPQYVIEKIYEISNGDAIICTEVGQNQMWAS
                     QFFDYTQPRTFISSGGLGTMGFGFPASIGAQLGRPDKRVIDIAGDGSIQMNIQELIVA
                     VQHRLPIIIAILNNGFLGMVRQWQQLFYNKRYSYTCINFQPDFVKLAQAYGAEGIRIE
                     KKADVVPAIQKAFTITDKPIILDFHVDREENVYPMIPAGKSVKDMMMRELA"
     gene            8907..9383
                     /gene="ilvN"
                     /locus_tag="PLX22_03865"
     CDS             8907..9383
                     /gene="ilvN"
                     /locus_tag="PLX22_03865"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT19066.1"
                     /translation="MSQHVISVKVENKFGVLARVAGLFSARGYNIDSLAVSETELPDV
                     SVMTIVVSGDDRIIEQVKKQLNKLIDIIKVTDHNQENAVQREIVLAKLNATPTKRNEI
                     YQLAQIFGARIVGVTNKAIILEMVSDPETVDNFIELLRPYSIKELIRTGRVSILKE"
     gene            complement(9449..10576)
                     /locus_tag="PLX22_03870"
     CDS             complement(9449..10576)
                     /locus_tag="PLX22_03870"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOT19067.1"
                     /translation="MHGHSIHDKSYKYLFSNPLIVKELLESFVAMEWIKEVDFSKAQP
                     VDKSYVNDDYKEYEADIIYKLMLKDAEIYLYLLIEFQSTVDNYMSFRMLTYIMELYRE
                     LIYKHKVKQLPVVFPILLYNGERKWTAATNIFQLINVPEQLQQCRPYIPQFQYYPIIE
                     NEILENELDAMMNVIATVFLLERGDKEIFLKTIDRIRNLMHIYGNENMIIRTLLVWLA
                     HYLETHGIIDNVKPIIESAFHPEEAYSMLSTTLQHLKEDLRKEGYTEGLQSGFQDGVQ
                     KGMQEGLQKGMQKGMQKGMQKGMQKGMQLGMQKGVVRGKQTGLKEGIVQGMIKERAKI
                     ASKMLRNGIDDATILKITKLDKNQLEILKNKNKNENKKKKK"
     gene            10918..11919
                     /gene="ilvC"
                     /locus_tag="PLX22_03875"
     CDS             10918..11919
                     /gene="ilvC"
                     /locus_tag="PLX22_03875"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT19068.1"
                     /translation="MAKMYYDNDADLSVISNKMIAVIGYGSQGHAQAQNLRDSGCKVI
                     VAELPGTPNYDIAVSHNFKPISAKEAAEKADIIQILAQDHVQAKLYENDVKPYLKKGK
                     TLVFSHGFNIHFGQIVPPSDVDVIMVAPKGPGHLVRSEYEKGGGVPCLIAIHNNATGK
                     AKEVALAYSKGIGATRAGVIETTFKEETETDLFGEQCVLCGGVSELVKAGFDTLVEAG
                     YQPEIAYFECMHELKLIVDLFYQGGINYMRYSVSDTAEYGDYMVGKRMVTEQTRKEMK
                     KVLDEIQSAQFARQWILENVAGRPQFNAIKKKEREHLIEKVGKELRKMMKWIDSKEV"
     gene            12122..13711
                     /locus_tag="PLX22_03880"
     CDS             12122..13711
                     /locus_tag="PLX22_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOT19069.1"
                     /translation="MSKKILLVYPEIPLTYWSFRYSLEFIGKKTVFPPLGLLTVAAML
                     PEDWEVELLDLNISSLDPTIIPDYDMVFISAMIVQQESFHHVTSMCKQYHVPVVAGGP
                     YPTSLWQSIPDVDYFVLNEAEVTLPMFLHDLQSGTLQHVYTSTQKPSLDKTPIPRFDI
                     IDLKPYSSVPLQFSRGCPFNCEFCDIISLFGRVPRTKSPQQFCAELDAVYATGFNESI
                     FIVDDNFIGNKAKVKELLKAIIEWQIAHAYPFSFQTEASVNLAHDDELMELMYKAGFD
                     MVFLGIETPVVSSLESVGKTQNTKENLLENVESIQQHGMEVSGGFIVGFDEDPDNVFD
                     LQIEFIQSSGIPMAMVGILDALPQTRLYHRLVEEGRLITESNGNNTHKLEPNFIPKMP
                     VQLLVDGYIKILKTIYSPKQYFERCLTLIKRLPGKRMVYRQFTLKEFKALILSLVKQG
                     FSSYGKDYFTFLLNTLKYRPKLFSEAIALAIKGYHLFKITDIMVNEYNKSLHKHGHDD
                     TIEKSLAATVPAGNEQLQTQL"
     gene            13738..14076
                     /locus_tag="PLX22_03885"
     CDS             13738..14076
                     /locus_tag="PLX22_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19070.1"
                     /translation="MSTPSTAVQILVTIIPIVGIVAGSVVLFFFLYFNHKQKILLIEK
                     GLYQKVSFDFDAFSLFTGFILLGAGASLTIFFLIKDGVSYALIGGLVPLGLGISFTCY
                     YFVKQKTTKQ"
     gene            14073..14630
                     /locus_tag="PLX22_03890"
     CDS             14073..14630
                     /locus_tag="PLX22_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT19071.1"
                     /translation="MNNISDEHIIQQILKGNQELFKIIIQRYEQLVYSLGMRFFKNHD
                     DASDFTQEVFVKVYGNLYSFKGTASFKTWMMKVCYNHAVNKIAQMQNNNTTEYHDTIA
                     DTSEPLNIFMKNELGELLEQAINALPPAYKVCIDLYFYAGLPFKDISRITGYPVNTIK
                     SYVFRAKQHLRDALKGTIAEDYHEM"
     gene            14620..15072
                     /locus_tag="PLX22_03895"
     CDS             14620..15072
                     /locus_tag="PLX22_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19072.1"
                     /translation="MKCKKAIETFLMLDNNEFIPLLLKFHIAHCKQCYNEISQLQKAF
                     TSMQTSSSSPSIHLEDIIMSQVMLQKRYDYTISHLNWITTGLIIFIGIATISYSDALK
                     WMTYHFGIQLVLPLYLVLGCIIAAYISSYVATHLKKLQAIAHSIKSLL"
     gene            complement(15069..>17134)
                     /gene="polA"
                     /locus_tag="PLX22_03900"
     CDS             complement(15069..>17134)
                     /gene="polA"
                     /locus_tag="PLX22_03900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT19073.1"
                     /translation="QSLDAIYNHLHELTGKIKEQIETQKDSAYLSRQLVTIKTDINIP
                     ISIENAKTPNLHNDTARQIFKKLEMDSIVRELGETEKSMTVEQKEGYCIVTDEETLHN
                     VIVQIKQAGLVSFDTETTSAKPMEADLVGVSFSIAEGSGWFMPVGDSSLWSQAAVPGE
                     NLLRHLKTVLEDKSIKKVGQNIKYDMLVMMHCGITFEGIYFDTMIASYLLNPGLRRHN
                     IDDLAQEYLNYTKVSYEELVKDGKKLQHITQVPMQKLSQYAIEDADIALRLYHILYQK
                     LEQENLLDLFFTVEMPLVTVLAYMEYYGVKIDLHHFEALSIELSNKLRDIEQKIYSLA
                     GVPFNINSTRELSHILFEKLGLKPVKKTKTGYSTDIQVLESLHGQHEIINYLIQYRTF
                     NKLQTTYVDTLPKLVNPKTGRIHTSFNQTVTATGRLSSSEPNLQNIPVRDEVGKKIRT
                     GFIVDKGCSMLSADYSQIELRLAAHLSKDPTMIEAFQKGIDIHAQTASSVFNVPLHEV
                     TEQMRRQAKIINFATIYGVSPYGLSQQADITVQQATEFIQKYFNAYPKIKEYIDTTIA
                     FAQKHGYVQTLLGRKRSIPEITSSNQFRREGAERVAINTPIQGTSADMIKVAMVTIHT
                     TFKEKQLKSKMILQVHDELVFEVPNDEKELIEEIVVSAMKNAITLDVPVVVDTGWGNN
                     WAEAH"
BASE COUNT         5193 a         3272 c         2831 g         5838 t
ORIGIN      
        1 aatttgtgaa tgtaatccca attttcttcc tccaatttta tttgcaattc cttctgcaaa
       61 agcgggtcaa caatttgccc aatctttttt ttaaaccata tttttgactg tggatcaaca
      121 ttctccagaa ttacctgcaa tttgtgcagt atacccatag ggaaggcacc gcttccgcat
      181 gccggatcta ttactttaat tttgtctatg gcatctatga tcgcttcttt atcatgttca
      241 ttaaggttag cagcagcttc aaaattttca tgtttcaata attcttcaat gatatctctg
      301 gatagttttg ttttctcaca caaatagttt ttaagcgatt catcaaccat ataatcaact
      361 atttcgcgcg gggtgtaata acttcctgta gctttgcgcg cattctcttt tgtttcaggg
      421 ttatattctg ccaaaaggtt ttcaaatatt ttacccaaca tttcaggatc aatggacact
      481 tcagtatcaa caagtgtatt ctcatcaatg gtaaaattat atcgctcaaa aacctcaaaa
      541 aggtttgcaa accagtcatt tgatattgtc agtatattta tatttggtga tattcccatt
      601 tcgtttattg cagttacagg ataataatca ctgtcgtgtg gttcaaataa acctccattc
      661 aaaaatggaa taagcgaata acttttagtt ttgtattttt ctaacctttc ttttaatggt
      721 ttatttaata tctgaaagaa taatggctca acatattcat ggtaatagtc tccattaaaa
      781 ttttttacca actcagatga aagatatttc tggggtatca atgaatcttt agaaccatcc
      841 ttcttcatct tcaaaaacca acaaaataaa agtctaccca gtaaccgaat ggcaaactct
      901 tccttttcat tttgatcttc ctttggaaat cgttgtaatc ccttgttatt gtagttgtta
      961 ataagtgtat aatataattc tactatattt ctataaaact ctttcgtaac cttttctaca
     1021 ctaaacgcgt caatgatttt ttctaaactt tcaaagcttg ctgcttgcat ttgctgcaaa
     1081 aatgtccggt ttgtctggtc tttacttaca tagtaggtgt aacgcctgaa gttactccag
     1141 tcctgcttgc catcgggacg gggtatgcta tagataaacg aaaacctgaa atttccattg
     1201 ctgtcgtaaa aaataaaaaa gccaccagtg tattgtatca tttgtttcaa tatctttttt
     1261 gcaaaggtgt attgcgcttt ttttccgctt ttttgtgtta attctttatt tacttttgcg
     1321 gcaatcacaa taaattcgtg aacatcgtta actttaaacg tgcctatttg cgtgctttca
     1381 tcaaaaagtt cgtcgttaca tccatctaat ggcttatctt gagatataaa atcatttgtt
     1441 tttttataaa aaaaattttt aaatttattt acgtcaaaat catttataat atcttcaagt
     1501 attctaacca catcatccat gctgctactc catttgttcc tttaatttta tattttattt
     1561 tctattgcaa taattatttc cttttgtaac ccatcttctt tagcttttat tttgtgtaag
     1621 taatcattgc ctaattcctg ctgcaatgca gcaatttcgt taaccagctc ctgtaacttt
     1681 gtagcatcgt tgtagtcaat gttgaaattt gcaatccgcc gcaatgtata atccgaaagg
     1741 gttccataat caataagatc ttctttaagc tgttttaaaa atggaatata cggttgcagt
     1801 gcctccgggc actcgttgct taagataaac tgtagcttat tctttgctct atcctgtata
     1861 ctcaacacac tttttggtaa gctggattta tctttatgca cttttacttt ttcatatgcg
     1921 ccccagaaac gttcgtctat tggcaaagcc ttttcatcaa tattgcatgc aattctatcc
     1981 actacttctt caagggtagt ttctacaata ctgccatccg aataatcata gttcataaca
     2041 aacaatctgt ttttcttaaa gaataccagc aattcatcat acgtgctttt ctttgatact
     2101 ttcactctgt gtggagtgct ttctatgttt tttataattt ctggatactg ggcctttata
     2161 ctttcaaata cattcaaaac tctggtatag aagctttccg cttctaatgt ttctggattt
     2221 atctgtaaac ggtgatagag ttcggctggt gttggttctt cgtcctcgtc aaaaatcttt
     2281 gcgtcctcac ccaatgtatt gtgtatcatg aacattttat tctgggctat ctcgcgcgat
     2341 ttaactatct cagcaccctt ttcggttggg aaaaagttta ctatatacag ctcatcaaac
     2401 acttttttgc ttatacggtt tatacgcccc aatcgttgaa taacacgcac tggattccac
     2461 ggtatatcat agtttacaat catacctgct ctgtttaagt taaatccttc ggaaagctta
     2521 tcggtacaca gcaatatgtc ataatcatct ttctgatcct gatacgaagc atcaaagttt
     2581 ttataaattt cttttatttt actatcagac aaatcaccgg aaataaccag aacgcgattg
     2641 ttaaatttat tatttaatgt atcttttaaa taacaaacag tatcaagaaa ctcagaaaaa
     2701 atcacaattt ttcgcttgtt tttatctttt tctttatttt gataaaattc atttgctaca
     2761 ttttttatta ggcaatccag ctttggatca ttgtttacca gatcaagcgc atcaagctgt
     2821 tccagtatgg aggtaaataa ctttatgtca tgttttatat catcaataaa tttttcttta
     2881 tatttaaagg tcttaatttt atatcgttta tatttttttg gtaaaacatc cttttgcagg
     2941 ttcgtttcat actcctgtaa atattgttct atttcatcaa cgtccatatc gtaaatcttt
     3001 tcaagcaatt gcctatccaa aataaattcg cctttaagtg gatctccacc tccggttttt
     3061 tcaataaact gtaatacatg ttcatggata cgtttaaaat tttttatact ctctttaaac
     3121 gcaccaaacg agctttcaaa ccgtttaaca agaagcctgc gcataaaatt aaaaaggttt
     3181 ttctgttgtt gatattcacg gtttttttct aaaagccctt cgtttacatc attatcatca
     3241 tttatttcaa gctcacctac ttcgtatata aatggccggt atatagatcc tttgaaagcc
     3301 tctacgtcat cctcaacaaa ataatgttca atgatagtat cataaaattg tagctgctta
     3361 ggtgttaatt cataaaacca ttctatcgga tcctttacaa tagataaatg ttgaacttca
     3421 tccttatagt acggattctt ttgtaaatca atgcgattac gtctaatcgt aactggttca
     3481 atgacatcgc gtatctgctt tgccagctta tgcgtttcag catttaccct gcgtatatct
     3541 atgttttctg ttccaaatag atatttgtaa aacgttgtag ctctatttct tttttcccga
     3601 tctttcgaat catgataccg ttttatataa cttagcttat caaatttaga tttatacatg
     3661 gaaaacaaaa gctcaaggtt gttttcaagg gtaatggttg attttctagg agtcacaaac
     3721 agttttatca atgaaaatat atccgaaggt cggttattaa aaggggtagc tgttaataaa
     3781 attactattt tatctctgca tatgtttttc agcagctcat acgaacgggt gtcctgattt
     3841 ctaaatctgt gcgcctcatc tacaatgaca acttcaatat ccttgcatct attcacaaat
     3901 tcaagtgctt cttctaattt cccgctggaa cgcacttccc aatcaaaaag cctgaaatct
     3961 tctaaatatt ttttccagcc catatcctta ttgtcattac caattaaccc gggtgggcat
     4021 attacaatgc ctcgtttttt taactgaaac gcaatggcac aggcaatgat agttttccca
     4081 agaccaacaa catcggccaa tataacgcca ttgtgttttt ctataatagc tatcgcctgt
     4141 gttattgcat ctaactgata gcggtatggc acgtagttgt tgcttatcag tatcctgttt
     4201 atcgcttcac tggcagtccg tatttcatac gtatctagat acgattttaa tacaaggcag
     4261 tatgcttcaa acggtgttat ttctttaata agtgtctggt gttgtaacaa atttataagc
     4321 tcttttttgc aggcatcatt ttcagtaata gcaaccgatt catcccatat tgtatcaaaa
     4381 tacctttcgg cttcttcgat accatagtca gttatttcta cattaaattc atgctggcca
     4441 ataagccctg cccgggttag gttgctgcta ccggttatta gtattttttg ttttacctgt
     4501 tcagttgaaa gtgtaaaaag gtacaatttt gcatgattgg gctggcgggt ctttcgtatt
     4561 attaatcgtt catttttaat taattgaata aaatatgcta tctgctcata aaaatcttta
     4621 ttgtcaaatt cttcgctatt tattgacttt gtaattgatt caaaatatct attaataaca
     4681 tctttgttgc ttacattagc atcattacca cattcaacaa tacaataatt tagagtatca
     4741 acgttaaggc caacacaaat ctttacaact acatcggcat tgttttgcaa tgaggtatac
     4801 aaatctttaa tccccgaaaa atagaaaaaa ccaactaaaa attttaattc tttgctatgt
     4861 gcaatcaaat ctcgaactct atgcagtaaa tttttagttg gttgattggt aataaagttg
     4921 gacataataa acctttacaa tgtaatatga tttttccgta cttactatta atccatgtaa
     4981 tatcctttta acatacaaca actatgggtt aatctgtata tacaatctaa ctttcctctg
     5041 tcaataactt tgtaatacaa atgagctatt tgtcgtcata ccctatatat tcctttactc
     5101 ttctatcata ttcgtttatt ttaacagtaa cagcatgcct ggtgtttgtg ccaatatact
     5161 gttcggcagc tacaaccata tagggaaata catatcgtaa gtcgttgcta ctgccaatgc
     5221 ttactatatc tgttttccat aactgctttt ccaccttttt agttttaaac aatttccagt
     5281 caaaagcaat taatataata gttctgctat agaatgttcg tttcacctgt gccactccag
     5341 ttattccgta tgtttgatta taatatgtgt ttccataata actgcccgat gaggatatat
     5401 tgccataggt tgttgaagat tctatccctg ttggtcccca tagtggaaca tttgcagtat
     5461 aattttgtgg accgcttata gcatacacaa gcatcacaac ctgatctgcc tcatcgatag
     5521 tattgactat tgcaaaccct ttttttatta ataccttttt aatatagttg ctaaactcct
     5581 gaaactgcaa atcattaata gcaacatcct tcattccggg tgcaacaaac actttattgc
     5641 ctgcagtctc attattattg atagacgata ccataacctc ataatgagca caccctattg
     5701 caaagaacaa cagcataata accatacttt tagctttcat agtataaacc tccaaaatag
     5761 tttgtatata aattaactac cgggcaggga catatactgt ccctgttaaa aatattttta
     5821 ctttgatttt acaaaccgca cgtatgcttc ttttattgct tcaagccaca tggtgcgtat
     5881 cgatgaaggt gcaacaatct cggcatattt gccataccgc aaaaccttcc ctaaaagctc
     5941 ctgaggatga tgaaccggaa gctgcagttc aacgtaggca ccgtactgtt catgttttcc
     6001 ttctttcatg gtctgctctt tatgccatac ctgttttttt actatattac ataccggttg
     6061 gtaaaaacga acctgcacta atttgccacc actgccttta taaatgccat atgttgactt
     6121 aataaatttt tgtaattcat catcattaat ttttcttgta cgctttttat ttacaacctc
     6181 tgctttctgt atgcgcgaca gtaaaaatgt tcgtatccct tttttagttt catcataggc
     6241 aatgacatac caccttccct cataatgaat aaagtgttcg ggggcgatag ttctggtagt
     6301 agtctttcct gtgacatcag catatttaat tacaatacaa ttatcattcc gaagcgttga
     6361 aaaaagtttg ctgaacagag taatatccaa ctgttcccat tcgtctattt cgtaggtaat
     6421 cctggacata agggggagat agtgagcaag aatattgcgc tttatcaggt cggggatagc
     6481 tttatccaca aaggggaaat gtgtgggact ttcaattaat ttcattaaga ataaataaaa
     6541 taacagcatg cgttcattgc ggtaggaaaa ataatcaaaa ggcaaagaat acaaatatcc
     6601 ttttttatat acatcatata ctattggtgc gttaatccgg tctctgaaat attcaagatc
     6661 gcgcttaacc gtccgtggcg acacttcaaa tttctccaca acctctttaa ctgtcacaac
     6721 tcccttttcg cgaatgctgg tatcaatata ctgcaaccgt tcattctgac tcaaggcaaa
     6781 cctcgtgcta atttttcttc aataattttt tattttaatt attataacat attatggtat
     6841 gttgtcaatt atcatttata tacatgtttg tgtaatgcta tacgggttac agcttttact
     6901 cacggtagta ttgctcttat ctgcaaatag cattatgcac tactgtcatt ttaggcttta
     6961 tcttgaatct ctttattacc atgtgatcct gaaaattaca ttgccgctac aatgtattat
     7021 atataaagaa aaccaattat cagtgactat cgcccactgc tattttttat aagtgtatgc
     7081 tatcaatgct tataatagtt taaatgttta tattatattt tatatattat tttatatttt
     7141 tataatttaa aaaaaattat gttgcaattt ttatgcctgc tatatttaga tatatgctta
     7201 ctttttattt atttctgcga ggtgtaacag gtgaaaatta caggtgcaca gattattatt
     7261 gaatcactta tcagggaaaa ggttgaagtc atgtttggct accctggcgg cgtggttata
     7321 cctattttcc accatttatt taatgcacct tttaaattta ttttatcccg tcatgagcag
     7381 ggggcgatcc atgctgctga tggttatgcc cgtgcaaccg gtaagcccgg tgttgtcatt
     7441 gcaacctccg gtccaggtgc caccaattta gttaccggca ttgcaacagc aaatatggat
     7501 tcgataccgc ttgttatatt caccggacag gtatccactg aggttattgg caatgatgca
     7561 tttcaggaag ctgatgttac agggataacc cgcccaatta ccaaacataa ttatttagtt
     7621 aaggatatta atgagttagc caaaattatt aaagaagctt tttatattgc aacaaccggc
     7681 aggcccggcc ccgtattaat tgatgttccc gtcaatatat caaatgccga aaccgagttc
     7741 cattatccag aaacagtgca tttgcgaagc tacaagcctg tcattcatgg tcattcaggg
     7801 cagattcaga aagcatgtaa attaattgaa caatcaaaac gtccagttgt gtatgctggt
     7861 ggtggtgtta tatcatccaa tgcatcggat gaattgcgta cattcataaa aaagacaggt
     7921 attcccatta ccactaccct gcttgggtta ggtgcctatc cggaaaatga caaactttcg
     7981 ctcaagatgt taggaatgca cggcacctat tatgcaaacc atgctgttca tcagagcgac
     8041 ctgcttatag ctgtgggtgc acggtttgat gatcgcgtga ctggtaaaat ttcagagttt
     8101 atacctcatg caaaggtcat ccatattgat atcgatccgt catcagttag taaaaatgta
     8161 gctgtcgatg taccaattgt tggtgatgcg aaacatgttt taaccgagat aaacaaaaca
     8221 gtaaaggctc ccaaaataga tgagtgggtt actcatgtaa tgaagatgaa agaagaaaac
     8281 ccgcttacct ataaagacag tgatgtaacc attaaaccac aatatgttat tgaaaaaatc
     8341 tatgagatca gcaacggtga tgctattata tgcactgaag ttggacaaaa tcagatgtgg
     8401 gcatcacaat tttttgacta tacacagccg cgaaccttta tatcatcagg cggactgggc
     8461 acgatgggat ttggatttcc tgcatctatt ggagcccaat taggccgtcc cgataaacgt
     8521 gtaattgaca ttgctggtga tggttcaata cagatgaata tacaggaact cattgtagca
     8581 gtacaacatc gattacctat tataattgct atactcaata acggcttttt aggtatggta
     8641 cgacagtggc aacaattgtt ttataataaa cgatattcat acacctgtat caatttccag
     8701 cccgattttg taaaattagc tcaagcctat ggtgctgaag gcattcgtat tgagaaaaaa
     8761 gcagatgttg tacccgcaat acaaaaagcg tttaccatca ctgacaagcc aataattctg
     8821 gatttccatg ttgatcgcga agaaaacgtt tatccaatga tccccgcagg gaaatctgta
     8881 aaagatatga tgatgaggga attagcatga gccagcatgt aatatcagta aaagttgaaa
     8941 ataaatttgg tgtgttagca cgtgttgctg gacttttttc tgcacgagga tacaacatcg
     9001 actcgttagc agttagcgaa actgaattac ctgatgtttc ggttatgact atagtcgtta
     9061 gcggtgatga ccgtattatt gaacaggtaa agaagcagct taataaactc attgatatta
     9121 taaaagtaac tgaccacaat caggaaaatg ctgtacagcg agagattgta ttagcaaaac
     9181 ttaatgccac acctacaaaa cgtaatgaaa tttatcaact ggcacagatt tttggagcac
     9241 gcattgttgg tgtcactaat aaagcaatta tactggaaat ggtcagtgat cctgaaacag
     9301 tagataactt tattgaactg ctgcgaccat attctattaa agaactcatc cggacaggca
     9361 gggtatcaat tcttaaggaa tgaaatcgta aaatttttta ttatatttgc tgcatcattc
     9421 atttaataat attagtgata tcaacccatt actttttctt tttcttattc tcattcttat
     9481 tcttattctt aagaatttcc agctgatttt tatccaattt tgttattttt agtattgtcg
     9541 cgtcgtctat cccattcctg agcatcttac tggcaatttt tgcacgttcc tttatcatac
     9601 cctgcactat tccttcttta agaccggttt gtttacctct gacaactcct ttttgcattc
     9661 ccaattgcat acctttttgc ataccttttt gcataccttt ttgcatacct ttttgcatac
     9721 ctttttgcaa cccttcttgc atccccttct ggacaccatc ctgaaaacct gattgcaagc
     9781 cttcagtata accttctttt cgtaaatcct ccttcaaatg ttgtaatgtc gttgataaca
     9841 tactataagc ctcctccgga tgaaatgctg attcaattat gggttttaca ttatcaataa
     9901 taccatgagt ttctaaataa tgcgccaacc atactaataa agttcgtata atcatatttt
     9961 cattaccata aatatgcata aggttgcgta ttctgtcaat tgtttttaag aaaatttctt
    10021 tatcacctct ttcaagcaaa aaaaccgtag ctataacatt catcattgcg tctaattcat
    10081 tttccaatat ttcattttca ataattggat aatattgaaa ttgcggtata tatggtctac
    10141 attgctgcag ctgctcaggc acattaataa gctgaaatat attagtagct gccgtccatt
    10201 tacgttcacc attatacaat aatatcggaa aaactactgg caattgtttt actttgtgtt
    10261 tataaatcag ttcacgatat aattccataa tataagtcag cattctgaac gacatataat
    10321 tatctaccgt tgattgaaat tcaataagca gatataaata aatttctgca tctttcaaca
    10381 tcaatttata tatgatatca gcttcatatt ctttgtagtc atcatttaca tagcttttat
    10441 caacaggctg agctttgctg aaatcaacct ctttaatcca ctccattgca acaaatgatt
    10501 ctaaaagttc ttttactatt aatggattag aaaaaagata tttatacgat ttatcatgaa
    10561 ttgaatgtcc atgcataaat aataaccacc tcctgctata aaattttatt caaatacata
    10621 caatttccat tgtatttctc tatgaaatta atacgctgaa actggaaaaa attaagggga
    10681 aaattaaaaa atattttttt ataattaaaa atttttaaaa tttttctgtt accaatagtg
    10741 agatatcaca ggaaatcgat aaataacact tttcaaacat taccctgttt taagaagtta
    10801 aaaaaaaatt aaatttttat tgcataatac aaatacaata atagactatg ctgcatcaat
    10861 aactgcatgt aagtatactg catttactct atataataat taaaacgagg tagcattatg
    10921 gcaaagatgt actatgataa tgatgccgat ttatcggtga tttcaaacaa gatgattgct
    10981 gtaataggat atggcagtca gggtcatgct caggcacaaa atctgcgaga tagtggctgt
    11041 aaagtaattg ttgcagaatt acccggaaca ccaaattatg atattgcagt ttcacataac
    11101 tttaaaccaa taagcgcaaa agaagcagct gaaaaagctg atatcatcca gatattggct
    11161 caggatcacg ttcaggcaaa gctttacgaa aatgatgtaa agccgtatct taaaaaaggc
    11221 aagacacttg tcttttcaca tggatttaat atccattttg gtcagatagt tccaccatca
    11281 gatgttgatg ttataatggt agcaccaaaa ggaccgggac atttagtacg tagcgaatat
    11341 gaaaaaggcg gcggcgttcc ctgtttaatt gcaatacata acaatgcaac cggtaaagca
    11401 aaagaagttg cattggctta ttcaaagggg attggagcta cacgagcagg tgttattgaa
    11461 acaacattta aagaagaaac cgaaaccgac ctctttggcg aacaatgcgt actgtgcgga
    11521 ggagtttcag agcttgttaa agcaggtttt gacacactgg tagaagctgg ctaccagccc
    11581 gagattgctt attttgaatg tatgcacgaa cttaaactca ttgttgattt attttatcag
    11641 ggcggcatta actatatgcg gtattctgta tctgacactg ccgaatatgg cgattacatg
    11701 gtaggcaagc gtatggtcac cgaacaaacc cgtaaagaga tgaaaaaggt acttgatgaa
    11761 attcaatcag ctcaatttgc ccgtcaatgg attttagaaa acgtagcagg ccgtccacaa
    11821 tttaatgcaa taaagaaaaa agaacgtgaa catctcattg agaaggttgg caaagagtta
    11881 cgaaagatga tgaaatggat tgacagtaag gaagtttaac aataactatc gaacagggcg
    11941 gtgataaatc atcgctctgt tcacatatcg aaattttttt gcaaccatcc tgatagataa
    12001 cctgtctata tagtaaattc aaaactaaac agattaataa atcagcagtt aataatatgc
    12061 atggttagtt ttccaaatat ataaactacc tgtttggttc aagacagcac aggaggttgt
    12121 tatgagcaaa aaaatattat tggtatatcc tgaaataccc cttacctact ggagttttag
    12181 atactcttta gaatttatag gtaaaaaaac agttttccct cctcttggtt tgttaaccgt
    12241 tgcagcaatg ttaccagagg attgggaggt tgagctactt gacttaaata tatcttcact
    12301 tgatcccaca ataataccag actacgatat ggtctttata tcagcaatga ttgtccaaca
    12361 ggaatcattt caccatgtta catcgatgtg caaacaatac catgtgcctg ttgtggctgg
    12421 tggcccctat cctacttctt tatggcaatc tattccggat gttgattact ttgttttaaa
    12481 tgaggcagag gtaacattgc caatgttttt acatgatctg caatctggaa cgctgcagca
    12541 tgtttatact tcaactcaaa agccatccct tgataaaaca ccaataccac gttttgatat
    12601 tattgatctt aaaccttatt ccagcgttcc attacaattt tcacgagggt gtccatttaa
    12661 ttgtgaattt tgcgacatca tctcattatt tggaagagtt cctcgtacaa aaagcccgca
    12721 gcaattttgc gccgaattag atgcagtata cgcaactggt tttaacgaat caatatttat
    12781 tgtagatgat aattttattg gcaataaagc aaaggttaag gaactactca aagcaatcat
    12841 tgagtggcag attgctcatg catacccatt ttccttccag accgaagcaa gcgttaattt
    12901 agcacacgat gacgaactta tggagcttat gtataaagca ggttttgaca tggtttttct
    12961 gggtattgaa acccctgttg tatcatcact tgaaagcgtc ggtaaaacgc agaacaccaa
    13021 agaaaatctt cttgaaaatg ttgaatcaat tcagcaacat ggtatggagg taagcggagg
    13081 ctttattgtt ggatttgatg aagatcctga taatgttttt gatctacaga ttgaatttat
    13141 tcaatcatcg gggataccta tggcaatggt aggaatactg gatgctctcc cgcaaacacg
    13201 gttatatcat cgtctggttg aagaagggag gcttattact gaatcaaatg ggaataatac
    13261 tcacaagctt gagccaaact ttataccaaa gatgccagtg caattgctcg ttgacggcta
    13321 tataaaaata ctgaaaacaa tctactcgcc aaaacaatat tttgaacggt gccttacatt
    13381 aattaagcgg cttccaggca aaagaatggt atatcgccaa tttacattga aagagttcaa
    13441 ggctcttata ctatcattag taaaacaggg attttcatca tacggaaaag attattttac
    13501 atttttgttg aataccttga aatatcgccc aaaattattt tctgaagcta tagcactggc
    13561 tatcaaagga tatcatttat ttaaaatcac tgatatcatg gtaaacgaat acaataaatc
    13621 acttcataaa cacggacatg atgatacaat tgaaaaatct ttggctgcca cagttcctgc
    13681 aggtaatgag cagctccaaa cacaacttta atattaactg atagggggaa ctattgtatg
    13741 tcaacaccat caactgcagt acagatttta gtaaccatca ttcctattgt tgggatagtg
    13801 gcgggcagtg ttgtattatt tttcttcctt tactttaatc ataaacaaaa aattcttctt
    13861 attgaaaagg gtttgtatca aaaagtatcg tttgattttg atgcatttag tcttttcacg
    13921 ggatttatac ttttaggtgc aggtgcaagt ttaacaattt tttttcttat taaagatggt
    13981 gtttcatatg cacttattgg cggattggtg ccattaggat tgggtatcag ttttacctgt
    14041 tattactttg taaagcagaa aacaaccaaa caatgaacaa tataagcgat gagcatatta
    14101 ttcagcaaat tcttaaaggc aatcaggaat tatttaaaat cattattcag cgttatgagc
    14161 agcttgttta tagtttgggt atgcgttttt ttaagaacca tgatgatgca tctgatttta
    14221 cgcaggaggt ttttgtaaag gtgtatggta atctctattc attcaagggg acggcatcct
    14281 ttaaaacatg gatgatgaag gtttgctata accatgccgt caataaaatt gcacaaatgc
    14341 aaaacaacaa taccactgaa tatcatgata ccatagctga tacatccgaa cctttgaata
    14401 tattcatgaa aaatgaactg ggagagcttc ttgagcaggc aatcaatgcc ctgcccccgg
    14461 catacaaagt atgcattgat ctctattttt atgcgggatt accttttaaa gatatatcac
    14521 gtataacagg atatcctgtc aatacaatca agtcgtacgt tttcagagca aaacagcatc
    14581 ttcgtgatgc attaaaagga acaattgcgg aggattacca tgaaatgtaa aaaagctatt
    14641 gaaacatttt taatgctgga taataatgaa ttcatcccat tgctccttaa atttcatata
    14701 gcacattgta aacaatgcta taacgaaatt agtcaattac aaaaagcctt tacatcaatg
    14761 caaacttcaa gctcctcacc atccattcat ttagaggata taataatgtc tcaggttatg
    14821 ctgcaaaaac gatatgatta taccatatcc catcttaact ggattacaac aggacttatt
    14881 attttcattg gtattgccac aatatcatac agcgatgcac tcaaatggat gacatatcat
    14941 tttggcattc agctggtgct acccttatat cttgtactgg gatgtatcat tgcagcgtat
    15001 atcagcagct acgtagcaac acatctaaaa aaattgcaag cgatagctca ttccatcaaa
    15061 tccttgcttt aatgagcctc tgcccaatta ttaccccagc ctgtatctac cacaaccggt
    15121 acatcaagcg taatggcgtt cttcatggcg cttacaacaa tttcctcaat taattctttt
    15181 tcatcattgg ggacctcaaa caccagctca tcgtgcacct gcagtatcat tttgcttttt
    15241 aattgctttt ctttaaacgt agtgtgtatt gtcaccattg caaccttaat catatcagca
    15301 gatgtcccct gaatgggagt gtttattgca acacgctcag caccctcacg tctaaactga
    15361 ttgctcgatg taatttcggg tatagagcgc tttctcccca gaagtgtttg cacatatcca
    15421 tgtttttgtg caaatgcaat ggtggtatca atatattctt taatctttgg gtatgcatta
    15481 aaatattttt gaataaactc cgttgcctgt tgcactgtaa tgtcagcctg ctgtgataat
    15541 ccatacggcg atactccata tatcgtagca aaatttataa tctttgcctg cctccgcatc
    15601 tgttcggtta cctcatgtaa cggcacatta aaaacagatg atgcagtctg tgcatgaata
    15661 tcaatgccct tttgaaatgc ctcgatcatg gtaggatctt ttgacaggtg agcagcaagc
    15721 cgtagttcaa tctgcgagta atcagccgat agcatgctgc atcctttatc aacaataaaa
    15781 cctgttctta tttttttgcc tacctcatca cgcaccggaa tattttgcaa attaggttct
    15841 gatgatgaaa gcctgccggt agccgttacc gtctggttaa acgatgtgtg gattcgtccc
    15901 gtttttggat taacaagctt tgggagtgta tcaacgtagg tggtttgtaa tttattgaat
    15961 gttcggtact gtattaaata attaatgatc tcatgctgtc cgtgcaatga ctccagcacc
    16021 tgtatatcgg ttgaataccc cgttttagtc ttcttaacag gttttaaccc taacttttca
    16081 aatagtatgt gcgatagctc tcgcgttgaa ttgatattaa atggtacgcc agccagcgaa
    16141 taaatcttct gctctatgtc ccgtaatttg tttgatagct ctatggacaa tgcttcaaag
    16201 tggtgtagat caattttaac cccataatac tccatataag caagcactgt aacaagcggc
    16261 atctcaactg taaagaacag atctagaaga ttttcctgtt ctaacttttg gtacaggatg
    16321 tgatagagtc gcaacgctat atcggcatct tcaatagcat actgcgacaa tttttgcatg
    16381 ggcacctgcg ttatgtgctg gagttttttt ccatctttaa ctaattcctc atagcttact
    16441 ttggtgtaat tgagatattc ctgcgctaaa tcatctatat tgtgtcgtcg cagtccagga
    16501 ttaagcaagt acgaagcaat cattgtatca aaataaattc cttcaaaggt aatcccgcaa
    16561 tgcatcatga ccagcatatc atattttata ttttgcccaa ccttcttgat acttttatcc
    16621 tcaagaacag tttttaaatg cctcagcaaa ttttcccctg ggactgctgc ctgactccac
    16681 agagaactat cgccaacagg cataaaccat cctgagcctt cagctataga aaatgaaact
    16741 cctacaagat cagcttccat tggtttggcc gatgttgttt cggtatcaaa tgatacaagc
    16801 cccgcttgtt taatctggac aataacatta tgcaacgttt cttcgtcggt tactatgcaa
    16861 tagccttctt tttgctctac cgtcattgat ttttcagttt cgccaagctc tcgtacaata
    16921 gaatccattt caagcttttt gaaaatctgt cgcgctgtat cattgtgaag gttgggcgtt
    16981 tttgcattct ctattgatat gggaatattg atatctgttt ttatggttac aagctgacgc
    17041 gataaatagg cactatcctt ctgcgtttca atctgctcct ttattttacc ggtaagttca
    17101 tgcaaatggt tgtaaatagc atccagcgat tgat
//
LOCUS       DAOSOX010000031        16937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3407_length_16937_cov_9.239900, whole genome shotgun sequence.
ACCESSION   DAOSOX010000031 DAOSOX010000000
VERSION     DAOSOX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3407_length_16937_cov_9.239900"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLX22_03905"
     CDS             <1..359
                     /locus_tag="PLX22_03905"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT19074.1"
                     /translation="EIVHNFMDFFVEESCGWCVPCRAGNVILKQKLEKIISGKASAKD
                     IQELEEWCRIVKATSRCGLGQTSPNPIYTTIQNFREIYEAKVKKDTDYVSTFDLLAAV
                     EESCKTAGRIPKVEEH"
     gene            361..1056
                     /locus_tag="PLX22_03910"
     CDS             361..1056
                     /locus_tag="PLX22_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT19075.1"
                     /translation="MSTINFTIDGKSCKAKPGQTIVEASKDNGIYIPTLCHFEGLKPA
                     GTCRICTVKVGGRNMAACTTPVTEGMVVENKTSELEDYRKAIVEMLFVEGNHMCPTCE
                     KSGNCELQALGYRYKMMAPRFPFLFPQRTLDASLPKIIIERNRCVQCLRCVRGITTSD
                     GKRIFGMVNRGGHVEITVDKELAANMSDDMAKKAMDLCPVGAILKKEVGFAIPIGKRK
                     FDAKPIGSEIEKM"
     gene            1091..1630
                     /locus_tag="PLX22_03915"
     CDS             1091..1630
                     /locus_tag="PLX22_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOT19076.1"
                     /translation="MSKPVVATASLAGCFGCHMSLLDIDDRILKLIELVEFNKSPIDD
                     IKTFTKQCDIGLIEGGCCNSENVENLKLFRKNCKILISVGECAIMGGLPAMRNGISVK
                     ECLEEAYLKCPTVDDNIIPNDDELPMILDKVYPCHEIVKIDYFLPGCPPRADLIWEAL
                     VALLTGKELDLPYEVVKFD"
     gene            1654..3102
                     /locus_tag="PLX22_03920"
     CDS             1654..3102
                     /locus_tag="PLX22_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOT19077.1"
                     /translation="MARKITIEPVTRVEGHGKVTIQLDPQNNVLESRFHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKAMDVIVGAGPDGLTPTAEKMRRLMHYG
                     QMFQSHALHFFHLVSPDLLFGVDADPAIRNVIGVALKFPDLAVQGVMMRKFGQEIIRA
                     TAGKKIHGTGAIPGGINKNLSIQERDEFLKGQDPMNITKMIEWAKAALDIFKDYHKKN
                     KDFIDSFATFPSSHLSLVRKDGALDLYHGVLRAVDSDGKKILNDVDYQDYLKYVGEEV
                     KSWSYMKFPYLKSLGKKDGWYRVGPLARLNTADFIPTPLAQKEFEEFKAYTKGKPNNM
                     SMHMHWARLIELLHSAEMIEQLLKDSDLQGDKLVAKGKKVKEGVGLIEAPRGTLFHHY
                     RINDNDQIEMANLIVSTTNNNEPMNRAVNWVAMNTINGKKEITEGMLNAVEVAIRAYD
                     PCLSCATHAIGNMPLEVSLVDADGNILDQKRK"
     gene            3105..3575
                     /locus_tag="PLX22_03925"
     CDS             3105..3575
                     /locus_tag="PLX22_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT19078.1"
                     /translation="MPDVKCLIYGYGNPGRQDDGLGIELATMVENNPQLKHHVDVDYN
                     YQLNIEDALTISEYDKVIFIDAAFNIDKPFVFYTIQPADTITFTTHYLSAESIVALCN
                     DLYKKQPESYMLAIQGYNWELGLPMSEQANSNLQLAYNFISEVILNRFSCCITG"
     gene            3609..3986
                     /locus_tag="PLX22_03930"
     CDS             3609..3986
                     /locus_tag="PLX22_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT19079.1"
                     /translation="MASKKLILVIDDDPDILDSIKAILTANAFDVVTAMSGKEGIEAV
                     SKTKPDLILCDMMMERIDAGTKVAQELKKDNPNLPIYLLSSIGTATATNIEIDKLGFN
                     GVFQKPVNPDNLVATIKNVLNIS"
     gene            complement(4073..5257)
                     /locus_tag="PLX22_03935"
     CDS             complement(4073..5257)
                     /locus_tag="PLX22_03935"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT19080.1"
                     /translation="MAIKYLDQINVNNKKVMIRADFNVPYDKNMNITDDTRITATLPT
                     IEYCLKQNAIVILVSHLGRPKGKIVPEMSLKPVAKRLSELLKKEVLFIDKPITQGIQE
                     EINKLKPGDVVLLENIRFYPEEEKNDESFGKLLGSLTDVYVNDAFATAHRGHASNEAI
                     TRYVKECVAGFLMKEELEYFKKALVHPEKPVTAIIGGIKISTKIDVLKNIIPKVNNLL
                     IGGGMSYTFLKALGYNVGKSVVEDDHLATAKEIMELAKKYNVNLMLPEDIIVANKFDS
                     PEGKEVVPINNIPDNKEGVDIGPITRKKFSDVIKQSKTIIWNGPLGAFETKDFADGTN
                     EIAKVVAESACTSVIGGGDTGAAINASGYANKMTYISTAGGAFLELMEGKVLPAVKAL
                     DK"
     gene            complement(5337..6326)
                     /gene="tatC"
                     /locus_tag="PLX22_03940"
     CDS             complement(5337..6326)
                     /gene="tatC"
                     /locus_tag="PLX22_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOT19081.1"
                     /translation="MAREQKPKNDSTKNNSGKKRKNSQKKETKVTRKKKSLSTNSEDL
                     SNNQQKQEKKNLIDELIKEADNDQTSKSEIEENEVDPAALSRGDKPMNIVDHLDEFRS
                     RLLIVLITILGLTIIGFYFSDILLDAINRPFIDSGYKLNIFNLVEGFTLRLKASFVSG
                     LLIGLPLLVYQLWQYIKPAVAIENRKFAKYSSVAAIFLFYGGLSMTYFYLLPLSIKVL
                     LNFTPPDMDNMINASKYFNFAVLFSIAIGITFEMPIIIMILTRIGIVSPSFLISKRKY
                     AIVLIWIFAAVITPTTDPLTLSLVALPLMLLYELSIIISKFMVIRKKRKELSQ"
     gene            6634..9651
                     /locus_tag="PLX22_03945"
     CDS             6634..9651
                     /locus_tag="PLX22_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT19082.1"
                     /translation="MFNDYTHLDAVVIPIIICDNKGKVKYVNNAFRTTKYSHSSDNEL
                     LNQQSVFTIFTLNENQKSEFKKVINNELNNITIILDTFQLLISKIKRNRTLVQIFKTH
                     KDFNTALHMYNANVDSFKNTFAPFFSAFDLLDDLIHITDKDGCIIYANKALNKLYGDI
                     KGQYCYSVFSKYDNYCPDCKMSLVINGSTIRYEAKLEKNGQYYDIINSPFIDFDGNIY
                     KISIMRNLEEIIDKQNTLLLFQNAINRSRNLVSFFKENGEISYANDTFIQYFGEQYKK
                     DINFLKLINCTNDELFYDVVDKEISLTLNNNLIFLKIFIYPIHSSDNEIIFVFIASDI
                     TNLKKLEKQLAVERNYFKSIIDNTIDGFFVINKNHNLIDCNPAFLKMFDLSYDNFKNI
                     RMEDLFDSQYKKDFDKKINYLNKTGISQITEVSHTKNGFKNYIISLNKLTSSEIDDAQ
                     YYGFVKDITDITRLHKIIENERNYNRRIIETVQLGFVVINEFGEIIDYNNAFLDLIGC
                     NGKNVKGEIYKSFIIPVSRDIMENFNVVSSNNPIEFEAIIQSSDNNNKNVIISMSKLY
                     DILGNYLGNFAFIRDITHQKKIENEILYQTNKVIELVNSLTQFTANVVRIDTIDNLLF
                     AISSFIQQLINPDYTEIFINNNTIFVSHKFNNGVHTVNNTEISQKTSFVVQKLTKNKT
                     ILYISNPLIELNDEDQLHFKNILEFQKIILIPIFIGDELKVILLSAFKTNEEKIDNVI
                     LTILTGVTNLLAITLEKIISVAEQNKMREALDRYDKLVAIGRIIAGVAHEINNPLSIM
                     QLDIAELNQKLQGDEYDNEIRDIIKSLFEEIQRISNIVKQLKDYSKPATLNDDIINLN
                     EIFRTYPLKILSKNIQKKGIPIVINVPKQKIFIKMPLTRLIQVIMNLLTNAEDAVIEK
                     GEGSIIITISQIQKDDICYACLSIQDTGIGIDEENMPLIFEPFFTTKPKEGTGLGLSI
                     SYSIIKSYNGEIEINSKKNMGTTVSVLLPAYKK"
     gene            complement(9855..11309)
                     /gene="ahcY"
                     /locus_tag="PLX22_03950"
     CDS             complement(9855..11309)
                     /gene="ahcY"
                     /locus_tag="PLX22_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOT19083.1"
                     /translation="MTQPSGKGMLNIAEYDHKLPFKVKDITMAELGRKELQLAENEMP
                     GLMALRKKYGNQKPLEGLRISGSLHMTIQTAMLIETLYHLGADIRWASCNIFSTQDHA
                     AAAIAQAGTAAVFAWKGETLEDYWWCTEQALIWPDGSGPDCIVDDGGDATLFVHLGVK
                     AENDSSIINKNYDSKDMQCLIDRLQLSVSKNPNRWTTIASKIRGVSEETTTGVHRLYQ
                     LEKSGELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMVAGKVVVVCGYGDV
                     GKGCAQSMRGFGARVIVTEIDPICALQAAMEGYEVKTLEDVVESGDIFVTATGCCDVI
                     RGEHMERMKDEAIVCNIGHFDSEIEMSYLENNDKCKRLNIKPQVDKWMLEKGHSIIVL
                     SEGRLVNLGNANGHPSFVMSNSFTNQTLAQIELATNQALEKKVYTLPKKLDEEVARLH
                     LGRLGVKLTRLNQKQASYLGVNIDGPYKPDHYRY"
     gene            11423..11959
                     /locus_tag="PLX22_03955"
     CDS             11423..11959
                     /locus_tag="PLX22_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOT19084.1"
                     /translation="MKCKNIFYVLSLILILSLSSCTPSQWHYRNIPLKRKQIINTATK
                     YIGIPYRHGGSNPSGFDCSGYVYYVYTKNGIPIPRSTTMQFKKGKKIPFRVAQPGDLV
                     FFSIQSSGVSHVGIFLGNDTFIHAPSSGKAIRYDLISNPYWKKRFVATVTYLNNYSQL
                     NEMKTIGSSYSEERFEWK"
     gene            12045..12524
                     /gene="coaD"
                     /locus_tag="PLX22_03960"
     CDS             12045..12524
                     /gene="coaD"
                     /locus_tag="PLX22_03960"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT19085.1"
                     /translation="MQIGIYPGSFDPLTCGHLDIIKRATQLFDRLVVAVARNSEKFPL
                     FSVEERLAMLSECCRDISKVELTSFDGLLAEYCKKNNINFIVRGLRAIVDFEYEYAIA
                     LMNKEIAPTVETIFLMSKSEFSFVSSKMVKEVAGYGGDISRLVPQFVNKKLKEKFQS"
     gene            12594..14129
                     /gene="rny"
                     /locus_tag="PLX22_03965"
     CDS             12594..14129
                     /gene="rny"
                     /locus_tag="PLX22_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT19086.1"
                     /translation="MSVLTILLIVALPLAAVSGYLFRAYIGKIKLTTAEAQSRKIIQD
                     AVKEAESKRKELLLEAKDQLLKERNQFEKEMRERRIELQNIERKILQKEEMIDNKIQQ
                     LEKQEKAVQLKEKENIAKEQELNKHLEKHKQELERISGLTREEAKQLLLKNLENEVRF
                     ESIKLINKVEEEARRSADKKAKEIVLAALQRSASDYTQESTITTVSLPSDDMKGRIIG
                     REGRNIRTLENLTGVDMIIDDTPEVVVISGFDPIRREIARLSLERLIENGRIHPARIE
                     EVVEKVKNELEENMLDEGEHAAFELGIPGLSRDALYYIGKLKYRSSYGQNVLSHSKEV
                     ANLAAIMAGELKLDVAIAKRAGLLHDIGKGSIVEGEGAHAIVGSELAKKFGENDIVVN
                     IIASHHNDKEPESFEAILVQVADAISASRPGARRESLDTYLKRLENLENIAYGFKGVE
                     KCYAIQAGREIRVMVSNEQVTDEEATILARDIASKIESELKYPGIVRVTLIRETRIVD
                     YAR"
     gene            14191..14994
                     /locus_tag="PLX22_03970"
     CDS             14191..14994
                     /locus_tag="PLX22_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOT19087.1"
                     /translation="MESLKVLFIGDIVGKPGRAIIKNYLSDICQEYSIGLVIANCENA
                     AGGMSITPEIANELFSYGIHVLTTGNHIFNNRNSIQLLETNTHFLRPANFPDGVPGTG
                     FTTLTINNITIAVINLIGRIGMEPVDCPFIKFNTIYETIQSTSDLILIDFHAEATSEK
                     KAFGWFVDGKATAVCGTHTHVQTADECILPQGTAYITDVGMTGPFDSVIGMDKDSSIH
                     NFIYHTRIKFKVANDDPKLNAVVITCNNVSKAVNIQRIIKSYEISTTNP"
     gene            15083..>16937
                     /gene="dxs"
                     /locus_tag="PLX22_03975"
     CDS             15083..>16937
                     /gene="dxs"
                     /locus_tag="PLX22_03975"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT19088.1"
                     /translation="MLDDINGPEDLKKLSIGELSSLSEEIRSRILEVVSHNGGHLASS
                     LGVVELTIALHYVFNSPTDRIIWDVGHQCYAHKIITGRNKLFHTLRMFHGISGFPKTS
                     ESVHDHYDTGHSSTSLSLALGEAIARDINSGNYQIVAVIGDGSLTGGMSFEALNHIGQ
                     SKKDIIIILNDNEHSISRNVGALSEYLMRLITGGVYNKLRRKSYEFIHRIPKIGPMLF
                     NLFDRIEARIKGIFVPGSFFEDLGIRYFGPIDGHNIPTLIDLFNRIAKLNKGPKIIHV
                     YTKKGKGYVHAEKNPSKFHGIGPFDIKTGKPLKKSSISYSDIAGKALVDVARRDKKIF
                     AITAAMKLGTGLSEFEDHFPQRFIDVGICEQHAITLASSLAKNGFKPFVSIYSTFMQR
                     AVDQIIHDVALMNLPVKLLIDRAGIVGDDGETHHGLFDIGIIRMIPNFVLLSPTNGNE
                     LRDMIHYASIYNNGPIAIRYPRGVDDKYVHSANSELIKLPLLKAYYKSIQPMIALFAV
                     GDMIKLATEVHMKLNEHDIKSSVYGILSLKPLPINQIEIILQNIPYYTVFENCYTSGG
                     ISEYLNSQINCSLRYKNIYNVAFPDNFILHGRVSELFAHYSMDSDSITNKII"
BASE COUNT         5867 a         2585 c         3024 g         5461 t
ORIGIN      
        1 ttgagatagt acataatttt atggactttt ttgttgaaga gtcatgtgga tggtgtgttc
       61 cttgtagagc aggtaacgtt atattgaaac aaaaactgga aaagataata tccggtaaag
      121 cttcagcaaa ggatattcaa gaactggaag aatggtgtag aatagttaaa gctacaagcc
      181 gatgtggtct tggacaaact tcacctaatc caatttatac aacaatccag aatttcagag
      241 agatatatga agcaaaagtt aagaaggata ctgattatgt atccacattt gatcttttag
      301 ctgctgtcga agaatcatgt aaaacagcag gaagaattcc aaaggtagag gaacattaat
      361 atgagtacaa taaattttac tatagatgga aaatcctgta aagcaaagcc aggtcaaact
      421 attgtagaag cctcaaaaga taatggcatt tatattccta ctttatgcca ttttgaaggt
      481 ttgaaacctg ctggtacatg tcgtatttgt acagttaagg ttggtggcag aaatatggct
      541 gcatgtacaa caccagtaac agaaggcatg gttgtagaaa acaaaacatc tgaacttgaa
      601 gattatcgca aagccattgt tgaaatgctt tttgtagaag gcaatcacat gtgtccaaca
      661 tgcgaaaaaa gtggtaattg tgagttgcag gctttaggat atcgctataa aatgatggca
      721 cctcgattcc catttttgtt tcctcaacga acgcttgatg cttctttgcc aaagataatt
      781 attgaacgca atagatgtgt tcagtgctta cggtgtgttc gtggtattac aacatcagat
      841 ggtaagcgta tttttggtat ggttaacagg ggtggtcatg tagaaattac agtggataag
      901 gaattagctg ccaatatgtc cgatgatatg gcaaaaaagg ctatggattt atgccctgtt
      961 ggtgctatct taaagaagga agtaggtttt gcaataccaa ttggtaaacg taaatttgat
     1021 gctaaaccaa taggtagtga aattgaaaaa atgtaatgat tatcataatg aagattgatg
     1081 gaggaataca atgagtaaac ccgttgtcgc aacagcttca ctggcaggtt gttttggatg
     1141 ccatatgtct ttactggaca ttgatgatag aattttaaaa ttaattgaat tggtagaatt
     1201 taataaatct ccaattgatg atatcaaaac atttactaag cagtgtgata ttggattgat
     1261 agaaggggga tgctgcaata gtgagaatgt tgaaaatttg aaacttttcc gaaagaattg
     1321 taagatatta atatcagtag gggaatgtgc tataatgggc ggattgcctg caatgcgtaa
     1381 tggcatttca gtgaaagaat gtttagaaga agcatacctt aaatgcccaa cagttgatga
     1441 taatataatt ccaaacgatg atgaattgcc aatgattctt gataaagtgt atccatgcca
     1501 tgaaatagtg aaaattgact attttttgcc gggatgccct ccacgtgcag atttaatctg
     1561 ggaagcattg gttgcacttc tgacaggtaa agagttagat ttaccatatg aagttgttaa
     1621 gtttgattaa tttttttcag gagatgaaat tatatggcta gaaaaataac gatagaacct
     1681 gtaacaaggg tagaaggtca tggtaaagtg accattcagc ttgatcctca gaacaatgtt
     1741 ttggaatcaa ggttccatat tgttgaattc agaggatttg aacgttttgt acagggcaga
     1801 ccatactggg aagcacctgt tttagtgcaa cgcctctgtg gaatatgtcc tgttagccat
     1861 catttagcag cagctaaagc gatggatgtt attgttggtg ctggacctga tggattaaca
     1921 ccaactgctg aaaagatgcg cagattaatg cattatggac agatgttcca atcacatgca
     1981 ttacactttt ttcatcttgt atcacctgat ttactgtttg gtgtagatgc tgatccagct
     2041 atcagaaatg ttattggtgt tgctctaaaa tttcccgatt tagcagttca gggtgttatg
     2101 atgcgtaaat ttgggcagga aataattaga gcaactgccg gtaagaaaat acatggtaca
     2161 ggtgccatcc ctggtggtat taataaaaat ttaagtattc aggaacggga tgagttcctt
     2221 aaaggtcaag acccaatgaa tattacaaag atgatagagt gggcaaaggc tgctttagat
     2281 atattcaaag attaccataa aaagaataaa gattttatcg atagctttgc aacattccca
     2341 tcaagtcatt taagccttgt tagaaaagat ggagctcttg atctttacca tggtgtttta
     2401 cgagcagttg attctgacgg taagaaaata ttgaatgatg tggactacca ggattatttg
     2461 aaatatgttg gtgaggaagt gaaatcatgg agttatatga aattcccata tctcaaatcg
     2521 ctgggtaaaa aagatggttg gtacagagtt ggtccgctgg cacgtctgaa tactgcagat
     2581 tttataccaa cgccattagc tcaaaaagaa tttgaagaat ttaaagcata tacaaagggc
     2641 aaacccaata acatgtcaat gcatatgcat tgggcacgat tgatagaatt attacattca
     2701 gctgaaatga tagaacagct tttaaaagat tcagatcttc aaggtgataa actggttgct
     2761 aaaggaaaga aagttaaaga aggcgttggc ttaatagaag cacctcgagg aaccttattc
     2821 catcattatc gtattaatga taacgatcag attgaaatgg ctaatttgat agtatcgaca
     2881 acaaacaaca atgaacctat gaatagagct gttaattggg tagctatgaa taccatcaat
     2941 ggaaagaagg aaataactga aggtatgctc aatgcagttg aggtagcaat acgtgcatat
     3001 gatccttgct taagttgtgc aactcatgca attggtaata tgcctcttga agtttcactt
     3061 gtagatgctg acggtaacat actggatcaa aaaaggaaat agcaatgcca gatgttaagt
     3121 gcttaatata cggatatggt aatcctggac gtcaggatga tggattgggt atagaactgg
     3181 caaccatggt agaaaataat ccacaattaa aacatcatgt tgatgttgat tataattatc
     3241 agctcaatat tgaagatgca ttgactattt cagaatatga taaagtaata tttattgatg
     3301 cagcatttaa cattgataaa ccatttgtat tttatacaat tcagccggca gataccataa
     3361 cgttcactac gcactatttg tcggctgaat ctatagttgc tctttgcaat gatttgtata
     3421 aaaagcaacc tgaaagttat atgcttgcaa tacaggggta taactgggaa ttaggattac
     3481 cgatgtcaga acaggcaaat tctaatttgc agcttgcata taattttatt tcagaagtta
     3541 ttttgaatcg tttttcatgt tgtattacag gttaaacttt attttagatt aaaatcagga
     3601 gtgatagtat ggcaagtaaa aaactgatat tagtaattga tgatgatcca gatattttag
     3661 attcaattaa agctattctt acagctaatg catttgatgt tgttactgca atgagtggca
     3721 aggaaggcat cgaagctgta agtaaaacca agccggattt aattttatgc gacatgatga
     3781 tggaaaggat agatgctggt acaaaagttg ctcaggaatt aaaaaaggat aaccccaatt
     3841 tacctattta tctattaagt agtattggaa cagccactgc aaccaatatt gaaattgata
     3901 aattaggttt taatggtgtt tttcaaaaac cagtcaatcc cgataatctt gttgcaacaa
     3961 ttaagaatgt gctaaatata tcataatatg cattgcttaa tcatttcgct tttcaataca
     4021 aaagcgggtt taattaaccc gcttttgtat taactaatat tggtgataga tactatttat
     4081 ctaaagcctt tactgcaggg agcactttcc cttccataag ttctagaaaa gcaccacctg
     4141 ctgtggaaat atatgtcatc ttatttgcat agcctgaagc atttattgca gcaccagtat
     4201 caccaccgcc aataacgctg gtacaagctg actcagctac cactttggca atttcattgg
     4261 ttccatcagc aaaatctttt gtttcaaatg ctcctaatgg cccattccat attattgttt
     4321 tggattgttt aatgacatcg gaaaattttt tgcgagttat tggacctatg tcaacaccct
     4381 ctttgttatc aggaatatta ttaatgggaa ctacttcttt cccttcggga ctatcaaatt
     4441 tgttagcaac aataatatct tcaggtaaca tcaaattaac attatatttc tttgcaagct
     4501 ccataatttc ttttgcagta gcaaggtgat catcttccac aactgatttg ccaacattgt
     4561 agcccaatgc ttttaaaaat gtatatgaca tgccaccgcc aataagaaga ttattaactt
     4621 taggaataat attttttaaa acatcaattt ttgtagaaat tttgatgcca cctattatag
     4681 cggttactgg tttttcagga tgtaccagcg ctttcttaaa atactccagt tcttctttca
     4741 ttaaaaagcc agcaacacat tcttttacat accgcgttat agcctcattt gaagcatggc
     4801 ctcgatgagc agtggcaaat gcatcgttaa catatacatc tgtcaatgaa cccaaaagct
     4861 taccaaaact ttcatcattt ttttcttctt ctggataaaa tctaatattt tcaagtaaaa
     4921 cgacatcacc tggttttagt ttgtttattt cttcctgaat tccttgagtt ataggtttgt
     4981 ctataaaaag tacttctttt tttaacaatt cagataaacg tttagctact ggttttagtg
     5041 acatttccgg gactatcttt ccttttggtc ttcctaaatg tgaaacaagt ataacaatgg
     5101 cattttgttt aaggcaatat tcaatagtag gtaatgttgc ggtaattcgt gtatcatcag
     5161 tgatgttcat gtttttatca tacggaacat taaaatcagc tctaatcata acttttttat
     5221 tgttgacgtt aatctggtca agatatttaa ttgccatatt tcacctcata aaatattatt
     5281 ttaaataatt ctattttaat tcaatatcaa aaaattatta aaaaaaagtc aaatagttat
     5341 tgcgatagtt ccttgcgttt tttccgtata accataaatt ttgatattat gatagacagt
     5401 tcgtataata acattaatgg aagagctaca agggatagtg tcaatggatc agttgtagga
     5461 gttattacag cagcaaaaat ccatataaga actattgcat atttgcgttt gctaattaaa
     5521 aatgatggtg aaacaattcc tatgcgtgtc aatatcataa tgataatagg catttcaaat
     5581 gtaataccta ttgcaattga aaaaagaaca gcaaagttga aatattttga tgcattaatc
     5641 atattgtcca tgtctggagg tgtaaagttt aaaagaactt taattgaaag aggtagaaga
     5701 taaaagtatg tcatagataa accaccataa aataaaaaaa ttgctgctac acttgaatat
     5761 ttagcaaatt tacgattttc aatggcgaca gccggcttaa tatactgcca taattgatat
     5821 actaaaagtg gcaaacctat tagcaaacca cttacaaacg atgcttttag cctcaaggta
     5881 aaaccttcaa caagattaaa tatatttagt ttatatcctg aatcaataaa tggtcgatta
     5941 attgcatcca gaagaatatc cgaaaaataa aatcctatga tggttaatcc caaaatagtt
     6001 atcaggacta ttaataacct agaacgaaac tcatccagat gatctactat attcattggt
     6061 ttatcacctc gactaagtgc tgcaggatca acttcatttt cttctatttc agatttagaa
     6121 gtttgatcat tatcagcttc tttaatcagt tcatctatca agtttttctt ttcttgtttt
     6181 tgttgattgt tgctaagatc ttcactgtta gtggataggg atttcttttt ccttgtgact
     6241 ttagtttctt ttttttggga attttttctt ttcttcccgg aattattttt ggtgctatca
     6301 ttttttggtt tttgttcacg tgccaatgtc atacctcaat attttaaaat atagaattat
     6361 gggcgcttct aaaaatgttt ttcataatca ttattgaaaa aatcatatac cattttacca
     6421 aacgcttgtg cccataaggg gacatcaaaa atataattat tagaagctgc tgtataattg
     6481 tataagataa cttaattatc taaaaaattg tcaagctatt ataataatta aaatttaata
     6541 tattgagtta ttttgataaa acttgacaat atactattaa tatagtatca taccatatct
     6601 taataatact attaactaag tgtttagtct aaaatgttta atgattatac acatcttgat
     6661 gctgttgtta ttcctattat tatttgcgat aataaaggca aagttaagta tgtaaataat
     6721 gcattcagaa caactaaata ttctcattca agtgacaatg aactattgaa ccaacaatct
     6781 gtttttacaa tttttacact taatgaaaat caaaaaagtg aatttaaaaa agttattaat
     6841 aatgaattaa acaatattac gattatatta gatacatttc aattattgat aagtaagata
     6901 aaaagaaatc gcacattagt tcaaattttt aaaacacata aggattttaa tactgcatta
     6961 catatgtata atgcaaatgt agatagtttt aagaatactt ttgctccatt tttttctgct
     7021 tttgatttac ttgatgatct catccatata actgataaag atggatgtat catatatgca
     7081 aataaagcac tcaataaatt atatggcgat attaaagggc aatattgcta ttcggtattt
     7141 agtaaatatg ataattattg tcctgactgc aagatgagtt tagtaataaa tgggagtacc
     7201 atacggtatg aagcaaaatt agaaaagaat ggtcaatact atgatatcat taattctcca
     7261 tttatcgatt ttgatggtaa tatttataaa atatctataa tgcgtaacct tgaagagatt
     7321 atagataaac aaaatactct cttattattt cagaatgcga ttaacagatc aagaaatctt
     7381 gtaagttttt tcaaggaaaa tggtgaaatt tcatacgcaa atgatacttt tatacaatat
     7441 tttggagaac aatataaaaa agatatcaat tttttaaaac tcataaattg tactaacgat
     7501 gaattatttt atgatgtagt tgataaagaa atatccttaa cacttaataa taatcttata
     7561 tttcttaaaa tatttattta tcccattcat tctagtgata atgaaattat ttttgttttt
     7621 atagcaagtg atataaccaa tttgaaaaaa ttagaaaaac aacttgctgt tgagcgcaat
     7681 tactttaaaa gtataatcga taataccata gatgggtttt ttgttattaa taaaaatcat
     7741 aatctcattg attgcaaccc tgccttttta aaaatgtttg atctatcata tgataatttt
     7801 aagaatattc gcatggaaga tctttttgat tcacaatata aaaaggattt tgataaaaaa
     7861 attaattatt tgaacaaaac aggaatatca cagattacag aagttagcca tactaaaaat
     7921 ggttttaaaa attatattat aagtttaaat aaattaactt cgtcagaaat agatgatgca
     7981 caatattatg gatttgtaaa agatataaca gatattaccc ggctgcataa aattattgag
     8041 aatgaaagaa attataaccg gcggattatt gaaacggtac agcttggatt tgtagtaata
     8101 aatgaatttg gtgaaattat tgactataat aatgcatttc ttgatttaat agggtgtaat
     8161 ggtaaaaatg taaagggtga aatatataag tctttcatca taccggtatc aagggatata
     8221 atggaaaatt tcaatgtagt atcatcaaat aatccaattg aatttgaagc aattattcaa
     8281 agttccgaca ataataataa aaatgttata atatcaatgt ctaaattata tgatatactg
     8341 ggaaactatc tgggaaattt tgcttttata cgagatatta ctcatcaaaa gaaaattgaa
     8401 aatgagatcc tatatcaaac aaataaggta attgagttag taaattcatt aacccaattt
     8461 actgccaatg ttgtacgaat agatacgata gataacctgt tatttgcaat atcatcattt
     8521 atacaacaac tcattaaccc ggattataca gaaattttta ttaataacaa taccatattt
     8581 gtaagtcata agtttaataa tggcgtacat actgttaata atactgaaat aagtcaaaag
     8641 acgtcttttg tagttcaaaa attaacaaaa aataaaacta tactatatat ctcaaatcca
     8701 ttaatagaat taaatgatga agatcaattg cattttaaaa atattcttga atttcaaaaa
     8761 atcatattga tcccaatatt cattggagat gaacttaaag ttatattgct cagtgcattt
     8821 aaaacgaacg aagaaaagat agataatgtc atattaacta tattaacagg tgttactaat
     8881 ttattagcta ttactttaga aaaaattata tcagttgctg aacaaaataa aatgcgtgag
     8941 gctttagata gatatgataa gttagtagcc attggtagaa taatagctgg tgtagcacat
     9001 gaaattaata atcctttatc gatcatgcag cttgatattg ctgaattaaa tcaaaaatta
     9061 caaggtgatg agtacgataa tgaaataaga gatattatta aatccctgtt tgaagaaata
     9121 caacgtataa gcaacattgt gaaacaatta aaagattatt ctaaacctgc aacgttaaat
     9181 gatgacatta ttaatctgaa tgaaatattc agaacatatc cattaaaaat tctttccaag
     9241 aacattcaaa agaaaggaat acccattgta attaatgtac caaaacagaa aatatttata
     9301 aagatgccgt taactcgttt gatacaggtc attatgaatt tactaaccaa tgcagaagat
     9361 gcagttattg aaaaagggga gggtagtatc atcataacta tctcacagat tcagaaagat
     9421 gatatatgct atgcctgttt atcaattcaa gataccggca ttggaattga tgaggagaat
     9481 atgcctctta tttttgaacc attttttact acaaaaccta aagaaggcac tggattggga
     9541 ttatctatat catattcaat tataaaaagt tataatgggg aaatagagat caatagcaaa
     9601 aaaaatatgg gaacaactgt gtcagtattg ttgcctgcat ataaaaaata gtacctgtat
     9661 agttgcatcg gctactaaaa aaaatatcta ttattacaag gtttcaatta tttactttat
     9721 gatatttact ttatgttgat agtaaagaaa taaaatcaat atatactaat tcaacagaat
     9781 aatataaaag caataataga ataaatagta tagtaaccat tacaggaact gaatggaagt
     9841 gcccctgcaa tgttttaata tcgataatga tctggtttgt atgggccatc aatattaact
     9901 cccagatatg aagcttgctt ttgatttaaa cgagttaatt taactcccaa tcttcccaga
     9961 tggagacgtg caacttcttc atcaagtttt tttggtagtg tataaacttt tttctctaat
    10021 gcttgatttg ttgcaagctc aatctgcgct aatgtctgat tggtaaaact attactcata
    10081 acaaagcttg gatgtccatt agcgtttcct aaattaacca atctgccttc tgaaaggaca
    10141 atgatagaat gtcccttttc cagcatccac ttatcaacct gtggtttaat attaagtcgt
    10201 ttacatttat cattattttc aagatagctc atctctatct cgctatcaaa atgtccaata
    10261 ttacatacaa ttgcttcatc tttcatgcgt tccatatgct cgccacgtat tacatcacaa
    10321 cagccggttg ctgtcacaaa tatatctcca gattctacaa catcttcaag ggttttgacc
    10381 tcatatcctt ccatagcagc ctgcaatgca cagataggat caatttcggt aactatcacc
    10441 cttgccccaa aaccacgcat ggattgtgca caacctttac caacatcacc gtaaccacat
    10501 acaacaacaa ctttgcctgc aaccattatg tccgtagcac gctttattcc atcggctagc
    10561 gattcccggc atccatacag attatcaaat tttgattttg taactgaatc gtttacatta
    10621 attgcaggga aaagaagctc tcctgatttt tcaagttgat agaggcggtg tacacctgtt
    10681 gttgtttctt cggatactcc acggattttt gaagcgatag ttgtccagcg atttggattt
    10741 ttagaaacag atagttgcaa cctgtcaata aggcattgca tgtccttgct gtcataattt
    10801 ttattgataa ttgatgaatc gttttctgcc ttgacaccta aatggacaaa cagagtggca
    10861 tctccgccat catcaacaat gcaatcagga ccactgccat caggccagat gagtgcttgc
    10921 tccgtacacc accaatagtc ttctaatgtt tcgcctttcc atgcaaaaac tgcggccgta
    10981 ccagcctgtg caattgctgc agcagcatgg tcctgtgtag aaaatatatt gcatgatgcc
    11041 cagcgtatat cagcaccaag gtgataaagt gtttcaataa gcattgcagt ttgaatggtc
    11101 atgtgcagac tgccacttat tcgcaaacct tccaatggtt tttgatttcc atatttttta
    11161 cgcaatgcca taagccctgg catctcgttt tctgctaatt ggagttcttt gcgaccaagc
    11221 tctgccattg taatatcttt aaccttaaac ggcaatttat gatcatattc agcaatattc
    11281 agcatcccct ttcctgaagg ttgagtcata caacacctca cataattatt aattattatt
    11341 taaatttttt tgtactattt cggcatttac cagctaaatt gtcaatataa ttattttatc
    11401 ctttcatgtc gatgtttatt atatgaaatg taaaaacatt ttttatgtac tatcgcttat
    11461 actcatatta tcattatctt cttgtactcc gtctcaatgg cattatcgta atataccgtt
    11521 aaaacgaaaa caaattataa atactgcaac aaaatacata ggtatacctt atcgccatgg
    11581 tggtagcaat ccctcaggtt ttgactgttc aggatatgtg tactatgtat atacaaaaaa
    11641 tggtatccct attccacgaa gtacaacaat gcaatttaaa aaaggaaaaa aaattccttt
    11701 tagagtagca cagccaggtg atctggtttt tttttctatc cagagttctg gtgtttcaca
    11761 tgttggaata tttttaggaa atgatacatt tattcatgct ccaagtagtg gaaaagctat
    11821 acggtatgat ttgataagca acccatattg gaaaaagcgt tttgtagcaa cagtgacgta
    11881 tttaaacaat tatagtcagt taaacgaaat gaaaaccatt ggttcatcat attctgaaga
    11941 acggtttgaa tggaagtaaa aaaaaattga aatatgaata aatgttgaca aaatgtaata
    12001 ataagattat tttagtagaa ccgatcagga taggttagtt ttatatgcaa attggaattt
    12061 atccgggatc tttcgatcct ttaacgtgtg gacacctgga tataattaaa agagcaacac
    12121 agttgtttga tagattagta gttgcagttg ctcgcaacag cgaaaagttt cctctttttt
    12181 cggttgaaga acgtcttgca atgctttctg agtgttgcag ggatatatct aaagttgaat
    12241 taacaagttt tgatggactc cttgcagaat attgcaagaa aaacaatatt aattttattg
    12301 ttagaggatt gcgggcgata gttgattttg aatatgaata cgccatagct ttaatgaaca
    12361 aggaaatagc ccctacagtt gagaccattt ttttgatgtc aaagagtgaa ttttcttttg
    12421 tatcatcaaa aatggttaaa gaggtggcag gctatggtgg agatatatcc cgacttgtcc
    12481 cacaatttgt gaataagaag ctcaaggaaa aattccaatc ctgatgcaac ctgtcctgaa
    12541 gctacatccg tagcagcaaa agttatattt aaaatcatgt aaggaggatt attatgagtg
    12601 tacttactat cctcctgatt gttgctctgc cattagcggc agtgtcaggc tatttgttca
    12661 gagcatacat agggaagatt aagcttacca ctgctgaagc acagtcaaga aaaataattc
    12721 aagatgctgt gaaagaagca gaatcaaaaa gaaaagagct tttacttgaa gctaaggatc
    12781 aattgctgaa agagcgtaat caatttgaaa aagaaatgcg tgaaaggagg attgaattac
    12841 aaaatattga acgtaagatt ttacaaaaag aagagatgat tgataataaa atacagcaat
    12901 tagaaaaaca ggaaaaagca gttcagttaa aagagaagga aaatattgca aaagaacagg
    12961 agttgaataa gcatttagaa aaacataaac aggaattgga aagaatttca ggattaaccc
    13021 gcgaagaggc caaacaacta ttgctgaaaa atttagaaaa tgaggttaga tttgaatcca
    13081 taaagctgat taataaagta gaagaagaag cccgaaggtc agccgataaa aaagcaaaag
    13141 agatagttct tgcagctttg cagcgcagtg cttcggatta tactcaggaa agtactataa
    13201 caaccgtttc cctgccatcg gatgatatga aaggaagaat aatcgggagg gaagggagaa
    13261 atatacgaac tcttgaaaac ttaaccggtg ttgacatgat tattgacgat acaccagagg
    13321 tggttgttat ttcaggattt gatcctatac gtcgtgagat agcacgctta tctttagaac
    13381 gcttaattga aaatggaaga attcatcctg caaggattga agaggttgtt gagaaggtaa
    13441 aaaacgaact tgaagaaaat atgctggatg aaggagaaca tgcagcattt gaattgggga
    13501 ttccgggtct ttccagagat gccctgtatt atattggaaa gttaaaatac cggtcaagtt
    13561 atggacagaa tgtactatca cacagcaaag aggttgcaaa cttagctgcc atcatggcag
    13621 gtgagttaaa gcttgatgtt gccattgcaa aaagagctgg attgctgcat gacataggca
    13681 agggaagcat tgttgagggt gaaggtgctc atgcgatagt tggctcggaa ttagctaaaa
    13741 agtttggaga aaatgatatt gttgttaata tcattgcatc gcatcacaat gataaagaac
    13801 ctgaatcatt tgaagcgata cttgttcagg tagccgatgc tatctcagcc tcacgaccag
    13861 gtgcaaggcg tgaatcactg gatacctatc tcaaacggct ggaaaatctg gaaaatatag
    13921 catatggatt taagggagtt gaaaagtgtt atgctatcca ggctggtaga gaaattagag
    13981 ttatggtttc taatgagcag gtgactgatg aagaagcaac aattttggca cgggacatag
    14041 cctcaaagat agaatccgaa ctaaaatatc ctggcatagt acgagtaacc cttatacgtg
    14101 aaaccagaat agttgattat gcacgataat ttgaatgtaa ctatacgaca agcaatgtgg
    14161 tacattgctt gtcgtatttt ataaaactca atggaatcac taaaagtatt attcataggc
    14221 gatattgttg gcaaacctgg tagggctata ataaaaaatt atcttagcga tatctgccag
    14281 gaatattcaa ttggacttgt cattgcaaat tgtgagaatg cagcaggtgg catgtctatt
    14341 acaccggaga tagcaaatga gcttttttcg tatggcatac atgtccttac aaccggtaac
    14401 cacattttta ataaccgaaa cagtattcag ctacttgaaa caaatacaca ttttttacgt
    14461 cctgcaaatt ttcccgatgg tgttcctgga actggattta caaccttaac aatcaataat
    14521 attaccatcg cggtaattaa cttaattgga aggataggga tggaacctgt tgactgtcct
    14581 tttattaaat ttaatacaat atatgaaact atacaatcaa catcagattt aatcttaata
    14641 gatttccatg cagaagctac ttccgaaaaa aaagcatttg gatggtttgt tgatggaaaa
    14701 gcaacagcag tatgtggtac acatacacat gtccagactg ccgatgaatg catattgcca
    14761 cagggaacgg cttatattac cgatgttggt atgaccggtc cttttgattc ggttattggc
    14821 atggataaag attcatctat tcataatttc atataccata cacgcataaa atttaaagtt
    14881 gcaaatgatg atcctaaatt aaatgcagtg gtaatcacct gtaataatgt aagcaaagca
    14941 gttaacatac agcggataat taaatcttat gagatatcga caacaaatcc ttgacaattt
    15001 atggcagggt taattttaat gatagcgata gtaaatatgt gatataatac attttaatat
    15061 attattttaa aaaagatact gtatgttaga tgatattaat ggacccgagg atttaaaaaa
    15121 actttcaata ggggaactat cttccctgag tgaagaaata cgctcacgca tacttgaagt
    15181 tgtatcccac aatggaggtc atctggcttc atcactgggt gttgtagaat taactattgc
    15241 cctccattat gtatttaatt ctccaactga caggattata tgggatgttg gtcaccaatg
    15301 ttatgcgcat aaaattataa cgggaagaaa taagctattt catacgttaa gaatgtttca
    15361 tggcataagc ggatttccta aaacatcaga gtctgtacat gatcattatg atacaggtca
    15421 tagcagtacc agcctttctt tagctttggg agaggctatt gctcgagata taaattcagg
    15481 taattatcag attgtagctg tcattggtga tggttcattg accggtggga tgtcatttga
    15541 agcattgaat cacattggac aatcaaaaaa agatattatc attatattaa atgataatga
    15601 gcattcaata tcaagaaatg ttggagcatt gtctgaatat cttatgcgcc ttataacagg
    15661 aggcgtatat aacaaattaa gaagaaaatc atacgaattt atccatagaa ttccaaagat
    15721 tggacccatg ctttttaatt tatttgaccg cattgaagct cgtataaaag ggatctttgt
    15781 gcctggaagt ttctttgaag atttgggaat acgatatttt ggtccaattg atggtcataa
    15841 tattcctact ttaattgatt tattcaatag gattgcaaag ttaaacaaag gaccaaaaat
    15901 tatacatgta tatacaaaga aagggaaagg gtatgttcat gcagaaaaaa atccctcaaa
    15961 atttcatggt attggtccat ttgacattaa aacaggtaaa cctttaaaaa aatcttctat
    16021 cagttattct gacatagccg gcaaagctct tgtggatgta gcaagacgag acaaaaagat
    16081 ttttgccatt actgctgcta tgaaattagg aaccggttta tccgaatttg aagaccattt
    16141 tccacaacgc tttattgatg ttggaatatg tgagcagcat gctataacat tggcttcctc
    16201 attagccaaa aatggattta aaccatttgt atcaatatat tctactttta tgcagagagc
    16261 tgttgaccag attattcatg atgttgcatt gatgaattta ccggttaaac ttctgataga
    16321 tagagcaggg attgttggag atgatggcga aacacatcat ggactttttg atataggcat
    16381 tatacgaatg atcccaaatt ttgttttgct ttcacctact aatggaaatg agctaaggga
    16441 catgatacac tatgcatcta tttataataa tggtcctata gctatacgct atcctcgagg
    16501 agtcgatgat aaatatgttc attctgcaaa tagtgagttg ataaaattac cacttttgaa
    16561 agcatattat aaatccatac agccaatgat tgctttattt gctgttggag atatgatcaa
    16621 acttgccact gaagttcata tgaaattgaa tgaacacgat ataaagtctt cagtatatgg
    16681 aatattgtcc ttaaaaccat tgcctattaa tcagattgaa atcattttac agaatattcc
    16741 ctattatact gtttttgaga attgttatac aagtggtggc atttcagagt acctgaattc
    16801 tcagattaac tgttcattgc gttataaaaa tatatataat gttgctttcc cagacaattt
    16861 tatacttcac ggaagagtat cagaattatt tgcacattat agtatggatt ctgattcaat
    16921 tactaataaa attatca
//
LOCUS       DAOSOX010000032        16662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3488_length_16662_cov_8.226832, whole genome shotgun sequence.
ACCESSION   DAOSOX010000032 DAOSOX010000000
VERSION     DAOSOX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3488_length_16662_cov_8.226832"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLX22_03980"
     CDS             complement(<1..318)
                     /locus_tag="PLX22_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOT19089.1"
                     /translation="MATKQLEVYKCDICGNIVEVLHEGKGELVCCGQPMKLQVENTVD
                     ASKEKHVPVAEKTSDGIKVQIGSVEHPMEEKHYIEWIQLIAGSKSYRQFLKPGEKPVA
                     IFKV"
     gene            complement(416..1405)
                     /gene="cydB"
                     /locus_tag="PLX22_03985"
     CDS             complement(416..1405)
                     /gene="cydB"
                     /locus_tag="PLX22_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOT19090.1"
                     /translation="MDVNIFQNIWFIIIGFLLMVYSILDGFDLGIASLMPVLAKDENE
                     QKKVMDSIWPVWDGNEVWLITAGASLFAAFPFAYATVFSGFYLALMLVLFALIFRAVS
                     MEFWYYDEKRRKIWATAFTAGSLLPALLFGVALGNVICGIPIDINMNFTGNFFTLLRP
                     FPLVIGLLGLSAILLQGCTWAIHKIEGEVAKRAHSILNKLLIANAVFFILAIVLTIIF
                     LPDILSSSIGWIGAIVYAVALSYVYLSNKHKRAVNALRGSSFAFIALWIMAGAALYPN
                     LVRATIGAYNITIVNASSTVYTLKIMSIIALIGMPIVIAYTFYVYKVFKGKVS"
     gene            complement(1425..2741)
                     /locus_tag="PLX22_03990"
     CDS             complement(1425..2741)
                     /locus_tag="PLX22_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOT19091.1"
                     /translation="MDVVMLSRIQFGLAAGVHFIFPSLTLGLTLLVLIYEYKFYKTDD
                     DIFKTISSFFIKILGLVFALGVATGIVLEFSFGTNWAEYSRMVGDIFGAPLAAEGIFA
                     FFLESVFLGVLLFARNRVSKKVYLLSAFLVFFGSHLSGLWIIIANSWMQTPAGFAIEG
                     GRAVLTNFWAAALNPSTLVRFAHVIIAGWITGSLFAAGIAAWYLIKRLHTNEAQHILK
                     TALIIFCIMSLAQFASGHIHSVQVTRIQPEKMATFEALWQTQQGAPMSIIGIPVESQK
                     KTYYEIKIPKLLSLLAYFDPNAEVKGRDAFPDDELPPVVLPYFSYHIMIGLGSLFALI
                     GIISIVLLLTKKLFNARWFWYVLFFAIPLPIIANEFGWIAAEVGRQPWAVYKVLKTAD
                     AVSIIVPASHVAFSLVILSLIYLMLIVMFIMILTKIIKKGPQSEIY"
     gene            complement(2820..2984)
                     /locus_tag="PLX22_03995"
     CDS             complement(2820..2984)
                     /locus_tag="PLX22_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOT19092.1"
                     /translation="MGKKYECTVCGYIYDPEEGDPDNGIQAGTAFKDLPDDWVCPICG
                     ATKEQFEPLD"
     gene            complement(3106..3303)
                     /locus_tag="PLX22_04000"
     CDS             complement(3106..3303)
                     /locus_tag="PLX22_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOT19093.1"
                     /translation="MYTFTVHDMMCNHCIMHITQSIQAIDKSSKVQCNLDSKTVTVDS
                     SLDINQIIKAIKDAGYTPVAQ"
     gene            complement(3605..4009)
                     /locus_tag="PLX22_04005"
     CDS             complement(3605..4009)
                     /locus_tag="PLX22_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT19094.1"
                     /translation="MEQYKHYLESKGISPSYQRLKILEYLDQNRSHPTVDEIYSALLP
                     LIPTLSKTTVYNTVSLFVQQGLVQMLLLDNVEARYDITVMPHAHLTCSQCGNVFDIDS
                     TPYVKFPQTIEGHTIEGYQIALRGICKNCLAD"
     gene            complement(4075..5250)
                     /locus_tag="PLX22_04010"
     CDS             complement(4075..5250)
                     /locus_tag="PLX22_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT19095.1"
                     /translation="MKPIALNDFIYWVGGIDWDLRNFHGYLTQRGSTYNAYLIMDEKI
                     TLIDTVKYYLMDEMLERISCIVDPSKIDIIISNHVEMDHSGGLPQLMELCPNAMLITS
                     PNGAKGLEAHYKKNWNYTVMKTGDSISLGKTSLQFILEQMVHWPDNMACYMPETKILF
                     SNDAFGQHIATSERFDTEYPLDIIMNEAKKYYANIVLPYDKQTQKVLDAVSTLDIAMI
                     APSHGILWKDHVQAIVTMYRSWAANKTTNKALIIYDTMWNSTKLIAHAIKNAFEEKNI
                     PVQLYDLKTIHISDIMTEVLDAHYICVGSPTLNNNMLPTVAAFLTYCKGLAPKNRVGL
                     AFGSYGWGGQSVAQVEDVLQQMGWRLLPAIKVNYVPQKQLLVTIAQEVYEKINEIDF"
     gene            5584..6876
                     /gene="eno"
                     /locus_tag="PLX22_04015"
     CDS             5584..6876
                     /gene="eno"
                     /locus_tag="PLX22_04015"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT19096.1"
                     /translation="MTIITDVHAREILDSRGNPTVEVDVELSSGIAGRFAVPSGASTG
                     EHEAIELRDGDKARYNGKGVLKAVQNVNEIISGAVVEMDALRQIEIDKTLIDLDGTPN
                     KAKLGANAILGVSMACAKAVAETLGMPLYQYIGGVGACELPVPMMNILNGGKHADNNV
                     DIQEFMIVPVGTDSFKEALRMGVETFHALKKVLTKKGYNTGVGDEGGFAPNLKSNAEA
                     FEVILEAIKEAGFKAGQDIYLAIDAAASEFYKNGKYVMAAEEKPEKTSEEMVNVYLEW
                     INKYPIISIEDGMAENDWDGWKLLTDKAKNTVQLVGDDVFVTNTSIFQKGIEKKVANS
                     ILIKLNQIGTLTETIEAIEMAKRAGYTAVISHRSGETEDPAIADLAVAMNTGLIKTGS
                     ASRTDRLAKYNQLLRIEEQLGVFAKFNGRRVFYSIGKP"
     gene            6839..7297
                     /locus_tag="PLX22_04020"
     CDS             6839..7297
                     /locus_tag="PLX22_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19097.1"
                     /translation="MAEGYFIPLVNHNTKFTLFIVILILFSVYFFVLSDSGILKRVIL
                     KHDIQHIQDQIALKQQQINKINRLDNNKMYSMYMLQESINAGYIPKGAKVYQFKDIPD
                     STKHREDSLFQTQHEKYLQYIQYGRIFWITFSALIMLGVLLHHRRKPVVL"
     gene            7308..7865
                     /locus_tag="PLX22_04025"
     CDS             7308..7865
                     /locus_tag="PLX22_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HOT19098.1"
                     /translation="MIPYNSKLNQRDLQVLQWQLKGTTDAVSGIFEKCNFGFPQIILL
                     SPIVNDLIDYQSLSNILWLTCPYLNDVIHQLENKGYITKIGNLINSDNQLKQEMAKAH
                     AHFYFLRKSLYEKYFGTDINDDYLNILKRGVGGLQDTIHIKCLHLHYAHYRICESNVA
                     GRIVYHLLQQKVNCDDAICRVSLQK"
     gene            7831..8499
                     /locus_tag="PLX22_04030"
     CDS             7831..8499
                     /locus_tag="PLX22_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOT19099.1"
                     /translation="MMQFVESRCKNDDIIIIKKFKKDGSIDSEVINRVSHILLQNGIV
                     VLPVDSQYEIVGMSNSIVEQKIRNLIKSRSKKFVRLITSFKMLESLATVSKLQYDFLH
                     RIWPGEVTVIVPQRQNTDKEIALRYPKTKFVHEIIERTGLPLIAVNIMHITRGSAQQQ
                     DIISNFQKKVDAIVIIEELCKRHPRPTLINIANGKLKIIRVGKVSSEEIQSYFYLGSS
                     DEEI"
     gene            complement(8496..9323)
                     /locus_tag="PLX22_04035"
     CDS             complement(8496..9323)
                     /locus_tag="PLX22_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19100.1"
                     /translation="MGNGNSHNELLAYRSYLENNIQYITEINKKIEKLILQLEKEHIS
                     INDCFVHLESYINILLNIYSRFTSLGGYDYCLKLKSLVQKYQQIENSEIQLSALYYIH
                     SKILSLQEEHIATFPSLTPKKDLYRELSPTKNHEEIDVANFAYKWLTFKRNNICFIVL
                     FDSYRIMDISQFNDFKALSQNFYVATIDNSTITIRDPLRLDEQPSRSIVLVTYARDTF
                     GYFYDTIGKKLGAQKNIIARMLTRTQSTSSLQYGRVRLFGDSYIYLKHVDITKDQTL"
     gene            complement(9329..>10138)
                     /locus_tag="PLX22_04040"
     CDS             complement(9329..>10138)
                     /locus_tag="PLX22_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT19101.1"
                     /translation="GLLVHLDLLDAFIPRSECSYSRVFHPDNFKQGENITAKIIDLDW
                     DKVKITLSIKEILPEPWSFINQFQIGNTYTGTVNKIIKNGAFVELKEGLEGFIPLSRM
                     SYSKKINDPHSVCSEGDTVQVTIIDIDESNKKITLELVTSEENPWLTINNTMTESIYT
                     ATIEEITSKGLVVNLPNQIRAFLPLEHCAIDKNADITKLYQNGNAIKVALLQVDADDR
                     RCLVSEKKAIEVEERSAYQQFAASNSSKGSTAFGQLFKKKYDELLDKGNKP"
     assembly_gap    10139..10238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10239..10622)
                     /locus_tag="PLX22_04045"
     CDS             complement(<10239..10622)
                     /locus_tag="PLX22_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT19102.1"
                     /translation="MSNININDENFSDLLEQSFIKGDSLSAGDVVKGTVILVTDESIF
                     VDIQAKSEAVLDAKEFIDDNGTKNIKAGDSIIAYVAAIKHGEIILTSAIGKGHISQDL
                     LYTAYKNHLPVYGVVKQSTNGGFIVS"
     gene            10843..11538
                     /locus_tag="PLX22_04050"
     CDS             10843..11538
                     /locus_tag="PLX22_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19103.1"
                     /translation="MRKSLYSIVVIVAVLIAANPGFSQEAKNTTLTSQNLTEESKQLD
                     QQILSICKRIEDTIATYKLMTIKDIRLLPFQVSYTQGNDFIYLEKHFFINDDIIPNKI
                     KLQKLKSIKIYTNGTTINKIESLIREENYYENSVTEVKIVDPSPNTLGTDDIVFTFVK
                     NNKPIVTDKKLSEVKNTLAFPIQNQLKNEFYIPHLMYFYDVLLNISETYYKGIKDIDS
                     TMTEFLKSSTKYY"
     gene            12052..13914
                     /locus_tag="PLX22_04055"
     CDS             12052..13914
                     /locus_tag="PLX22_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT19104.1"
                     /translation="MSLIVDRRDQEFVLYEMLGLEELFSTPRFADYSKDMFDMAIDLS
                     KRISEEEVLTSYMEGDREGAKLINGEVKVPHCYHHLHKTMNNSGLFTMAVSPEAGGQG
                     FPYVIDLAAREYYVFNMGFLLYPEAAVGAAHLIEVYGTEEQKNKYMYKMYEGKWGGTM
                     VLTEPEAGSDVGNLKTKAIPQPDGTYRIVGSKIFISGGDSDLFENIVHPVLARIEGDP
                     SGTAGISIFLVPKYLVNDDGTIGERNDVTITGIEHKMGLKGSATCSMSFGDNGNCYAE
                     LLGNPRQGMKIMFQMMNEARIGMGLQGLGTASIAYLHALNYAKERKQGADLMNMQNPE
                     APRVPIINHPDVRRMLLWMKSHVEGMRALVYLCALAIDRKEASEGDEAEKWHGIMELL
                     VPITKAYCTDMGFRVTELAIQIYGGYGYCQDYPVEQFMRDLKIGSIYEGTNGIQALDL
                     VGRKMAQKKGANFINFLGEINKTLTRYKDNPRLTDISKDIHDAVNILADMGMFFVQCG
                     KEGKFLIPISNAYSFLNLMGTVTLGWLLFWQSGIAYEKLDAIGKENHIDVNDANALAQ
                     LAKEHKEAAFYCGKIHSARYYIAHVLPHAQSYAKAIKSQDISMLHIPEESFATV"
     gene            14365..15807
                     /locus_tag="PLX22_04060"
     CDS             14365..15807
                     /locus_tag="PLX22_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOT19105.1"
                     /translation="MGYKDLKDFINTLEKAGQLKHIIPEVDAYLEITQIADRVSKDGG
                     PALLFEKVKGSQYPVLINAFGSYQRMQMALQCNSFDEIGEKIYSLITMQPPKNFMDKV
                     KALLTLKDIARIMPEVVGTAPCQKHVLMQGALLDALPILTCWPKDGGPFLTLPIVITK
                     DPETGLQNAGMYRMHKFSNTTTGMHWQYNKDGARHYRKYCQLQKQMPVAVALGGSPSI
                     IYAATAPLPPDVDEMLFAGFLGSEPVELVKAKTVDLLVPAHAEFIIEGYVNPGDETME
                     GPFGDHTGFYSAADIYPVFHVTCITTSDNPVYPATIVGKPPMEDCYMAKATERIFLPF
                     LKMVIPEIADIELPLEGVFHNCALVSIKKEYPAQAKKVINALWGLGQMASTKYVVVFD
                     DDVNLRDYSTVVWKLLNNVDPRRDLMIVEGPLDALDHSAPFANFGGKMGIDATRKTKE
                     EGMGRDWPDEITMRDDIIALVAKRWKEYGF"
     gene            15797..16642
                     /locus_tag="PLX22_04065"
     CDS             15797..16642
                     /locus_tag="PLX22_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOT19106.1"
                     /translation="MDFKKLSQLILIEQTAFALPFAYLGILFAGGGTLTTWIFASLAL
                     VAARTAGMCFNRLIDAEIDAKNPRTKERALPRGEVTKQEVLIVAVISCVVFIFSSYML
                     NMLCFYLSFLAIVLLVSYSYFKRFSSASHLYLGVVEAAAPIGGYLAATGEFTLIPFIL
                     GCAIMMWIAGLDVVYALLDVDFDRQEGLYSIPSRYGVKTALIISSLLYLLSFSSLIIA
                     GMLTNMKEAYWISVLCVGIIFIYQQMLARRKDIEPAIKEFFKVNSFIAPVLFLGTFID
                     VYFIV"
BASE COUNT         5247 a         2940 c         3283 g         5092 t
ORIGIN      
        1 aaccttaaaa attgcgacag gtttttcacc cggttttaaa aactgtcggt atgacttgct
       61 ccctgctata agctgtatcc attcaatata gtgcttttcc tccatgggat gttcaacgct
      121 accaatctga acttttattc catcagatgt tttttccgca accggcacgt gtttttcttt
      181 tgatgcatct acggtatttt caacctgcaa tttcatgggc tgtccgcagc atacaagctc
      241 gcctttacct tcatgcaata cctcaacgat attgccacaa atatcacatt tatacacttc
      301 taattgtttt gtagccattt actcctcctt gtaaaaataa tatttaacga aaaaatataa
      361 acctgctttg atacttcatt cactattcat tcagtaaatg atgataactg cactatcatg
      421 acactttccc tttaaaaacc ttatacacat aaaaagtata ggcaataact atgggcatac
      481 ctatcaacgc tatgatggac attatcttca aggtgtacac cgttgaagag gcattcacaa
      541 tcgtaatatt ataggcacct attgttgctc gtaccagatt gggataaaga gcagcgcctg
      601 ccattatcca cagtgcaata aatgcgaagg atgatcctct cagtgcattc actgctcgtt
      661 tatgcttatt tgacaaatat acatagctta atgctactgc ataaacaata gcacctatcc
      721 atccaatact acttgaaagg atgtcaggca gaaaaatgat agtgagaact atcgccagaa
      781 taaaaaatac ggcgttagca ataagtaatt tgtttagaat gctgtgtgca cgttttgcca
      841 cttcaccttc aattttatgt attgcccatg tgcatccctg cagaagtata gccgatagtc
      901 ctaacaaacc aataacaagt ggaaaagggc gcaacagtgt aaagaaattt ccggtaaagt
      961 tcatgttgat atcaataggg attccacata taacattacc aagggcaaca ccaaatagta
     1021 acgcaggtaa aaggctgcca gcagtaaaag cagttgccca tatttttctg cgcttttcat
     1081 catagtacca gaattccatt gaaacagctc taaatatgag agcaaacagc accagcatga
     1141 gtgctaaata aaaaccactg aaaacggttg cataagcaaa tggaaaagca gcaaataatg
     1201 atgcaccagc tgtaatgagc catacttcat taccatccca tacaggccat atagagtcca
     1261 taaccttttt ttgttcattt tcgtctttag ctaatacagg cataagactg gcaatgccta
     1321 aatcaaatcc gtcaagaatt gaataaacca ttaacaaaaa acctataatg ataaaccata
     1381 tattctgaaa tatatttaca tccatattca aacctccata ctcattagta tatttctgat
     1441 tgcggtccct ttttgataat tttagtcagt atcataataa acatcactat aagcatcaga
     1501 tagattaatg ataatataac caatgaaaaa gctacatgcg aagcgggaac aattattgat
     1561 actgcatctg ctgtttttaa tactttataa actgcccatg gttggcggcc aacctcagca
     1621 gcaatccatc caaactcatt ggcaatgatt ggtaaaggga ttgcaaaaaa caacacatac
     1681 cagaaccatc ttgcattgaa tagttttttt gtaagcagca acacaatact tattatccct
     1741 ataagcgcaa acagtgaacc caatccaatc ataatatggt atgaaaagta ggggagcacc
     1801 acaggtggga gttcatcatc agggaacgca tcccttcctt taacttcagc gttgggatca
     1861 aaataggcaa gtaaactaag aagtttggga attttaattt cataatatgt tttcttctgg
     1921 ctttccacag ggatgccaat tattgacatt ggtgcgccct gttgtgtttg ccacagcgct
     1981 tcaaaggttg ccatcttttc gggctgtatt ctggttacct gtactgaatg gatatggcct
     2041 gatgcaaact gcgccaatga catgatgcag aatataatca atgcagtttt gaggatatgt
     2101 tgagcttcat ttgtatgtaa tcgctttatt aaataccatg cagcaatgcc agcagcaaaa
     2161 agcgaaccgg taatccagcc ggctatgatt acatgggcaa accgcaccag cgtggaaggg
     2221 ttaagtgcag cagcccagaa gttggttaaa actgctctac caccttcaat ggcaaagcca
     2281 gcaggggttt gcatccatga attggcaatg ataatccata aacctgaaag atgcgagcca
     2341 aagaatacca gaaacgctga aagcaaataa accttttttg ataccctgtt gcgggcaaaa
     2401 agcagcaccc ccagaaatac tgattcaagg aaaaaagcaa atatcccttc tgctgcaagt
     2461 ggcgcaccaa atatatcgcc aaccattcgg gaatattcag cccaatttgt accaaatgaa
     2521 aattcaagta caataccggt tgccacacct aatgcaaaca caagccccag tattttaata
     2581 aaaaatgaac taatagtttt aaaaatgtca tcatctgttt tgtaaaactt atattcatag
     2641 ataagcacta aaagggtaag ccccagtgtt aatgaaggaa atataaagtg aacaccagcg
     2701 gccagtccaa attgtatacg tgaaagcatt acaacatcca tacataaacc tccagagaaa
     2761 tatttttata aatataacgg atatgcttac ctgagcatat cccgacagtt tacagtattt
     2821 taatccagcg gttcaaattg ctctttggtg gcaccacaaa tagggcaaac ccaatcatcc
     2881 ggcaaatctt tgaaagcagt tccagcttgt atgccgttgt cgggatcgcc ttcttcagga
     2941 tcataaatat aaccgcaaac cgtgcattcg tactttttac ccatataaat aattcctcct
     3001 tatattgtat tgtaaattgt aagatgaaaa atgcaaaatc taaagtgtta aatgcaaaaa
     3061 tgtaaagtaa ataaattgct catcgccatt caaaacacaa tcaatttatt gtgcaacagg
     3121 tgtatacccc gcatccttaa ttgctttgat aatttgattg atatcaagag aactatctac
     3181 tgttactgtt tttgagtcaa gattgcattg tacttttgaa gatttgtcaa tagcctgtat
     3241 ggactgggta atgtgcataa tgcaatgatt acacatcatg tcatgtaccg taaatgtata
     3301 cataaaaagc tcctataaac aataagagta ttgggcaaca ctattttaca tcatatcaca
     3361 catccaatca agtgaagcac aacaataaac cattgttaat gctgtgttct caatgacagt
     3421 ggcatcacaa tgttttggta aagctttaca gcatatgtca ctcatcactg cgatgaaatg
     3481 taaaaaatcg atacatttgc cagtaactat cgccacaaaa atactttaaa ttataattat
     3541 cacatagtta taataattac aaatattttt ttagtaaagc atattttttt gttgccataa
     3601 ctccttaatc tgccagacag tttttgcaaa taccacgtaa ggctatttga taaccttcaa
     3661 ttgtgtgccc ttcaatcgtt tgtggaaact ttacatatgg tgtgctatca atatcaaaaa
     3721 cgttaccgca ttgtgaacag gtcagatgtg cgtggggcat aacagtaatg tcataccgtg
     3781 cttctacatt atcaagcagc agcatctgta caagcccctg ttgaacaaaa aggctgacgg
     3841 tattataaac cgttgtttta gacagcgtag ggatgagcgg aagcagtgcg gaatagattt
     3901 catcaacggt tgggtgcgac ctgttctggt ctaaatattc cagtatcttt aatcgctggt
     3961 acgatggcga gattcctttt gattctaaat agtgtttgta ttgttccata gtggttaccg
     4021 ttataatggt attatgaact cattgcatgc ttttataaaa tacaaagttt tatgttaaaa
     4081 gtcaatttca ttaatttttt cataaacctc ctgggcgata gttaccagca attgtttttg
     4141 tggcacataa ttaactttaa tagcgggaag caatcgccag cccatctgct gcagtacgtc
     4201 ctctacctgc gcaacgctct ggccgcccca accatacgac ccaaatgcca gtcccacacg
     4261 gttctttggt gccagacctt tgcagtaggt taagaatgca gcaacggttg gcagcatgtt
     4321 attgttaagc gttggtgatc caacacagat atagtgtgcg tcaagtactt cggtcataat
     4381 gtctgaaata tgtatagttt tcaaatcata aagctgtacg ggaatatttt tttcctcaaa
     4441 ggcatttttt atagcgtggg caattaattt tgtggaattc cacatggtgt cgtagatgat
     4501 gagagctttg ttggttgttt tattggcagc ccagctccta tacatggtta ctatcgcctg
     4561 aacatgatct ttccatagta tgccgtgact tggtgcaatc atggcaatgt ctaacgtact
     4621 tacggcatca aggactttct gtgtttgctt gtcatagggc aaaacaatat tggcatagta
     4681 tttttttgct tcattcatga taatgtcaag gggatattct gtatcaaagc gttctgatgt
     4741 tgcaatatgc tgtccaaagg catcgttgga aaacaaaatt ttagtctcag gcatataaca
     4801 ggccatgttg tcaggccagt gaaccatctg ctcaaggata aattgcaagc ttgtttttcc
     4861 taatgatatg ctatcgccgg tcttcatgac ggtatagttc cagttttttt tgtagtgagc
     4921 ttccaatccc tttgcaccgt ttggcgaggt tataagcatg gcattggggc acaattccat
     4981 aagctgtgga agtccgcctg aatgatccat ttcaacatgg tttgatatga taatatcaat
     5041 cttagaaggg tcaacaatgc atgaaatgcg ttcaagcatc tcatccatga gataatattt
     5101 aacggtatca ataagggtaa tcttttcatc catgattaaa taggcattgt atgttgaacc
     5161 acgctgtgta aggtaaccgt gaaagtttcg caaatcccag tcgataccgc caacccagta
     5221 tataaagtca ttcagtgcaa ttggtttcat tgtacgctcc tttttattat actatggtaa
     5281 ttattgcaat agttaaattg atagcaactg tgctgaattg ttcaagaatt attttgtatg
     5341 aaatatattt ttcagtgatg caacccccct ggccctccct ttatcaaagg gatggtaacg
     5401 ttgtaaattg gctatccgtt agtaaaggga gggttaaaaa taactatcga acatatattc
     5461 ccattatact ataaaccaaa aactaaaatt atacttgcac attacagcta tcaatgattg
     5521 cattgattat gaaaatgtat tacttataga taatgtatat tataattatg aaggagttgt
     5581 atcatgacta ttataaccga tgtccatgca cgtgagattt tggattcacg gggcaatcct
     5641 actgtagaag ttgatgttga gctttcttca gggattgctg gcaggtttgc tgtgccgtca
     5701 ggtgcatcaa ctggcgaaca tgaggcaatt gaattaaggg atggcgacaa agcacgttat
     5761 aatggcaagg gtgtgcttaa ggcagtacaa aatgtcaatg aaattatatc aggcgctgtc
     5821 gttgagatgg atgcattacg tcagattgag attgataaaa cgttaataga tcttgatggc
     5881 acaccaaaca aagctaaatt aggggctaat gcaattttag gtgtatcaat ggcatgtgcc
     5941 aaagcggttg ctgaaacatt ggggatgcca ttatatcaat acattggcgg agttggagca
     6001 tgcgagttgc ctgtcccaat gatgaatatc ctgaatggtg gaaaacacgc tgataataat
     6061 gttgatattc aggagtttat gatagttccg gtaggtaccg atagtttcaa agaagcattg
     6121 cgcatgggtg ttgaaacatt ccacgctttg aaaaaagtat taaccaaaaa aggatataat
     6181 accggtgtag gtgatgaagg aggttttgca ccaaatttaa aatccaatgc cgaagctttt
     6241 gaggtaattc tggaagcaat aaaagaagca ggctttaaag ccgggcagga catttacctg
     6301 gctatagatg ctgcagcaag tgaattttat aaaaacggta aatatgtgat ggcagccgaa
     6361 gaaaaaccag aaaaaacatc tgaggagatg gtcaatgttt atcttgaatg gataaataaa
     6421 tatccaatta tttcaataga agatggtatg gccgaaaatg attgggatgg ctggaagctg
     6481 ttaactgata aagcaaagaa tacggtacag cttgttggtg atgatgtatt tgttaccaac
     6541 acatcaatct tccagaaggg tattgaaaaa aaggtagcca attccatact tataaaatta
     6601 aatcagattg gtacgttaac cgaaactatc gaagcaattg aaatggcaaa gcgtgctggt
     6661 tacactgctg ttatatcgca tcgttcaggt gaaactgaag atcctgctat tgctgattta
     6721 gcagttgcaa tgaatactgg attaattaaa acaggttcgg cttcccgtac tgaccgttta
     6781 gcaaaatata atcagcttct tcgtattgag gagcagcttg gtgtttttgc aaaatttaat
     6841 ggcagaaggg tattttattc cattggtaaa ccataacaca aaatttacat tgtttattgt
     6901 tatacttatc ctcttcagtg tgtacttctt tgtattgagc gatagtggca tactgaagag
     6961 agtaattctt aaacatgata tccagcatat acaggatcag atagctttga aacagcagca
     7021 gattaataag atcaatcggc tggacaataa taaaatgtat tcaatgtaca tgttacagga
     7081 aagcatcaat gctggttata tccccaaagg agcaaaggtt tatcagttca aggatatacc
     7141 agatagtaca aaacatagag aagactcatt atttcaaaca caacatgaga aatatttgca
     7201 atatatccag tatggacgaa tattctggat tacattttct gctttgataa tgttaggagt
     7261 gttgctccat catagaagaa aacctgtagt gctataatga atactatatg ataccataca
     7321 attctaaact taatcagcga gatttgcagg ttttacaatg gcagcttaaa ggaaccactg
     7381 atgctgtttc cggtatattt gaaaaatgca actttggatt tcctcaaatt attcttttat
     7441 cacctatagt taatgattta atagattatc aatcattgtc caatattctg tggcttacat
     7501 gcccctacct caatgatgtc atccaccagc ttgaaaataa aggatacata acaaaaattg
     7561 gaaatttaat caattcagat aatcagttaa aacaggaaat ggcaaaagca catgctcatt
     7621 tctacttttt aagaaagagt ttatacgaaa aatattttgg tactgatatt aatgatgact
     7681 atttaaatat attgaaaaga ggcgttggtg gattacagga tacaatacat ataaaatgct
     7741 tgcatttaca ttatgctcat tatagaattt gtgaaagtaa tgttgcggga aggattgtgt
     7801 atcatttatt acagcaaaag gtaaattgcg atgatgcaat ttgtagagtc tcgctgcaaa
     7861 aatgacgata tcattatcat aaaaaaattt aaaaaagatg gtagtatcga tagtgaagtt
     7921 ataaacaggg tatctcatat tttacttcaa aatggtattg ttgttcttcc tgttgattct
     7981 caatatgaga ttgttggtat gtccaacagt attgttgaac aaaaaatacg aaatttaata
     8041 aaatccagat caaaaaaatt tgtacgactc attacatcat ttaaaatgtt agaatcgctt
     8101 gcaacggtaa gtaagttgca atacgatttt cttcacagga tatggccagg ggaagtaaca
     8161 gttattgttc ctcaacggca aaatacagac aaagaaatag cattgcggta tcctaaaaca
     8221 aagtttgtac atgagataat tgaacgcaca gggcttccac tcattgctgt taatattatg
     8281 cacattacca gaggttcagc ccagcagcag gatataatca gcaattttca gaaaaaggtt
     8341 gatgctattg taattattga agagttatgt aaacgtcatc ctcgtccaac tctcatcaat
     8401 attgcaaatg gaaaacttaa aataataaga gttggcaagg tgtcatctga agaaatacag
     8461 tcatattttt atctgggaag cagtgatgaa gaaatttaaa gtgtctggtc ttttgttatg
     8521 tcaacatgtt tcaaataaat ataactatcg ccaaaaaggc gtacccttcc atattgtagc
     8581 gacgatgtgc tttgtgtcct tgttaacatt cgtgcaataa tatttttttg tgcacccagc
     8641 tttttcccga tagtatcata aaaataacca aaggtatctc ttgcataggt aacaagcaca
     8701 attgagcgcg aaggctgttc atcaagcctc agggggtcac gtatggtaat ggtgctgtta
     8761 tcaattgtag caacataaaa attttgtgaa agcgccttaa aatcattgaa ctgtgagata
     8821 tccataatac ggtaactatc aaataaaaca ataaagcaga tgttgttgcg tttaaacgtt
     8881 aaccatttgt aagcaaagtt tgccacatca atttcctcat ggttttttgt gggcgatagt
     8941 tccctgtata ggtctttttt tggcgtaagt gaagggaatg ttgcgatatg ctcttcctgc
     9001 aatgacagta tttttgagtg aatgtaatag agcgcagaaa gttgaatctc agaattttcg
     9061 atctgctgat atttctgtac aagtgatttt agctttaaac aatagtcata tcctccaagc
     9121 gacgtaaaac gcgagtagat gtttaataat atattaatat aactttcaag atgtacaaaa
     9181 cagtcgttta ttgaaatatg ttctttttca agttgcaaaa taagtttttc aattttttta
     9241 tttatctcgg ttatatattg aatattattt tcaagatatg acctgtaggc taaaagttca
     9301 ttgtgtgaat tgccatttcc catgataatt atggtttatt acctttatca agcagttcat
     9361 catatttctt tttgaaaagc tggccaaatg cggttgatcc tttgctggaa tttgaagcag
     9421 caaattgctg gtatgcactg cgttcttcaa cttcaattgc ttttttctct gatactaaac
     9481 atcgcctatc atcagcatcc acctgtagta aagcaacttt aatggcatta ccattctggt
     9541 acaattttgt aatgtctgca tttttgtcga tagcacagtg ctctagtggt aaaaatgctc
     9601 gtatctgatt tggcaaattc actacaagtc cttttgatgt aatctcttca atcgttgcag
     9661 tatatattga ttcggtcata gtattattaa tagtaagcca cggattttct tcactggtta
     9721 ccagttcaag tgttatcttt ttgttgctct cgtcaatatc aataatggta acctggacag
     9781 tatcaccttc gctgcaaaca ctgtgtggat cattaatttt tttactatag ctcatacggg
     9841 ataggggtat aaaaccttca agcccttctt tcagctcaac aaatgctcca tttttaatta
     9901 ttttgttgac agttcctgta taggtattgc ctatctggaa ttggttaata aagctccatg
     9961 gctctggtaa aatttccttt atactcaagg taatttttac tttatcccaa tctaaatcaa
    10021 taatctttgc tgtaatgttt tcaccctgtt taaaattatc agggtgaaat acccgactat
    10081 atgaacattc acttcgagga ataaaagcat caagtaagtc cagatgtacc aacagcccnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg atacaataaa tccgccattg
    10261 gtgctctgct ttacaacgcc atataccggt aaatggtttt tatatgctgt atacagcaaa
    10321 tcttgtgaga tatgtccttt gccaatagcc gaagttaaga ttatttcacc atgttttata
    10381 gcagcaacat acgctataat actatcacca gcttttatat tttttgtacc attatcatct
    10441 ataaattctt ttgcatctaa tactgcctca ctttttgcct gaatatcaac aaaaatactt
    10501 tcgtcagtca caagaatcac tgttccctta acaacatctc cagctgaaag gctgtcaccc
    10561 tttataaatg attgttcaag caggtcagaa aaattttcat cattaatatt aatgttgctc
    10621 attgctattt cctttcagca cttaaaattt cagtaatgta ttaaatgtat ggaatggcgt
    10681 ataaattgtc aatattgttt ttattggtaa ttattttctt cataaaaatt atattgacat
    10741 ttgataatag atggcagtat attagtaaaa attttatagt aagcaatttg acttacatta
    10801 tttagtgaga tatgttttca aattattcta cggaggatat acatgcgtaa atcgttatac
    10861 agtattgtgg tgatagttgc tgttttaata gctgctaatc ctgggttttc acaggaagct
    10921 aaaaatacta ctttgacatc ccaaaattta acagaagaaa gtaaacaatt ggaccagcaa
    10981 atccttagca tttgcaaacg gatcgaagac acaatagcta catacaaatt gatgacaatt
    11041 aaagatatac ggttgcttcc atttcaggtt tcgtacacgc aggggaatga ttttatttat
    11101 cttgagaaac acttttttat taatgatgat atcataccca ataaaataaa attacagaaa
    11161 ctaaaatcca ttaaaatata tactaacgga accactatta ataaaataga gtcacttatt
    11221 agagaggaaa attattacga aaatagcgtt acagaggtaa aaattgtcga tccatcccct
    11281 aatacattgg gaacggacga tatcgttttt acctttgtta aaaataataa accaatagtt
    11341 actgataaaa aactttcaga ggtaaaaaat actctggcat tccccatcca gaatcagtta
    11401 aaaaacgaat tttatatacc tcatctgatg tatttctatg atgttttgct gaatatatcc
    11461 gaaacatatt ataaaggaat aaaagatatt gatagcacca tgaccgaatt tttaaagagt
    11521 tcaactaaat attattaaca gtgttacaat attataaata aatagcaaaa cgaaaataga
    11581 aataaacctc acccctgtcc cctctccttc gcaaggcgag gggagcattg tttccaggtt
    11641 attctgatgg acgcagtccc gaagaatcca ttcgttcaca aaaagttgag atttttcact
    11701 ccgttcagaa tgacatgtca tcactcccgt cattctggac ttaacccgga atcaaaatat
    11761 gtaacgttag attcttaaat aagttcagga catgattcag gatgatgtcc ttgccattgt
    11821 cattctgagg gagcgccagc gactgaagaa tctgttcatt cacaaaacgt cgaaattctt
    11881 cgccttcggc tcagaatgac atgtttacca ttaccttatg agtggcggga tatccggcca
    11941 ttgaaacttt attgagagca cttataaaat tataataaaa tattgtgaat gtataattgc
    12001 taactatatt tatatatata aattaatata attataacaa tgaggttgta tatgagcttg
    12061 atagttgaca gaagagatca ggaatttgtg ttgtacgaga tgctaggatt ggaggagttg
    12121 ttttcaacgc ctcgttttgc cgattattca aaggatatgt ttgatatggc aattgacctg
    12181 tcaaaaagaa tatccgaaga ggaagtattg acttcgtata tggaaggtga cagggaaggt
    12241 gcaaaactga ttaatggtga ggtaaaggtt ccccattgtt atcaccattt gcataaaaca
    12301 atgaataata gtggactttt taccatggcg gtatcgcccg aagctggagg acagggcttc
    12361 ccctatgtaa tagatctggc tgcacgtgag tactatgtgt ttaatatggg ctttttgtta
    12421 taccctgaag ctgctgttgg cgctgctcac ctcatagagg tatacggcac tgaagagcag
    12481 aagaataagt acatgtataa gatgtatgaa gggaaatggg gtggtacgat ggttttaact
    12541 gaaccggagg caggttcaga tgtcggtaat ttgaaaacaa aagctattcc tcaacctgat
    12601 ggcacctaca ggatagtagg aagtaagata ttcatatcag gaggtgattc cgaccttttt
    12661 gaaaatattg ttcatcctgt actggcgcgt attgagggtg acccttcagg aactgcaggt
    12721 atttcaatat ttcttgtccc aaaatatctg gtgaatgacg atggaacaat tggggaaagg
    12781 aatgatgtta ctataaccgg tattgagcat aaaatgggat taaagggaag tgccacctgc
    12841 tcaatgagtt ttggcgacaa tggcaattgt tatgctgaac tgttagggaa tccacgtcag
    12901 ggcatgaaga taatgtttca gatgatgaat gaagcacgca ttggaatggg tttacaggga
    12961 ttgggtactg cttccattgc ctatttgcac gcattgaact atgcaaaaga acgcaaacag
    13021 ggtgcagatt taatgaatat gcaaaatccc gaagcaccac gagtacctat tataaatcat
    13081 cctgatgtac gcaggatgct gttatggatg aaatcgcatg tagaaggtat gagagccctg
    13141 gtatatttat gtgcattggc tattgaccgt aaagaggcaa gtgagggtga tgaagcagaa
    13201 aaatggcacg gcattatgga attgctggta ccaattacca aagcgtattg taccgatatg
    13261 ggatttagag taaccgagct tgcaatacaa atttatggcg gttatggcta ttgccaggat
    13321 tatccggttg aacagtttat gcgtgatttg aagattggat caatatatga aggtactaat
    13381 ggcattcagg cattagacct ggtgggaagg aagatggcac agaagaaggg tgctaatttt
    13441 atcaattttt taggtgagat caataaaaca ttaacacgtt ataaagacaa tccacgatta
    13501 acagatatct caaaagatat tcacgatgct gttaatatac tggctgatat gggtatgttc
    13561 tttgttcaat gcggtaagga aggcaaattt ttaattccaa taagcaatgc atattcattc
    13621 ctgaatctaa tggggacggt tactcttggc tggttattat tctggcagag tggcattgcc
    13681 tatgaaaagt tagatgctat cggtaaagaa aatcatattg atgtcaatga tgcaaatgca
    13741 ctggcacagt tagcaaaaga gcataaggaa gcagcatttt attgtggcaa aattcatagt
    13801 gcacgctatt atattgctca tgtgttgcct catgcacaat cgtatgcaaa ggcaataaag
    13861 agtcaggata tttcaatgtt acatattcca gaggaaagct ttgcgactgt ttaaacaggt
    13921 agttgtatgt atcataaaaa ggcgggcgct gtttgcttgc cttttttatt atatgaatcg
    13981 agtgagcaac aatgacatgc cccaattcga gtatgtggta aggctctgac cccacttttt
    14041 aaccatacta taattgtacc atataatgtt tatagggatt aatatgcgtg agggatgcgg
    14101 agggtggcaa agccagccac aggtgttgca ggcatagtat gcccaattgc aggggcgcat
    14161 cgtgatgtac aaggtagggg tatataatta tatgcattgt tatgtaaaac acctgtggcc
    14221 atagcccgca gcagcccgac cccgcgtcag cgggggcacg cccggatgac acagaattgt
    14281 ataacacact atacaatgaa aaaatacttg catttaacaa ttttgctata caatgaggtg
    14341 catttacata ataaagaggc aaccgtggga tataaagatc tgaaagattt tattaacacg
    14401 ttagaaaaag ccgggcagct taaacacatt ataccagagg ttgatgcgta tcttgaaatt
    14461 acccagattg ctgaccgcgt gagcaaagat ggtgggccgg cacttctttt tgaaaaggta
    14521 aagggttcac agtatcctgt acttatcaat gcatttggca gttaccagcg catgcagatg
    14581 gcgttgcagt gcaattcgtt tgatgagatt ggtgaaaaga tttattcgct cattacgatg
    14641 cagccgccta aaaattttat ggataaagtt aaagccctgc ttacattgaa ggatattgcc
    14701 aggattatgc cagaggtagt tggtacggcc ccctgccaga aacatgtact aatgcagggt
    14761 gcgttgcttg atgctctgcc cattttgaca tgctggccta aggatggcgg gccgttttta
    14821 accctgccga tagttataac aaaggacccg gaaaccggtt tgcagaatgc tggcatgtac
    14881 cggatgcata aatttagtaa cacaacaacc ggaatgcact ggcagtataa taaagatggt
    14941 gcacgtcatt accggaaata ttgtcagttg cagaaacaga tgccggtagc cgtggcactg
    15001 ggaggcagtc cttctataat ctatgctgct acagcaccgc tgccacctga tgtagatgag
    15061 atgctttttg ctggttttct ggggtcagag cctgttgagc tggtaaaagc aaaaacagtt
    15121 gatttgctgg tgcctgccca tgccgagttt atcattgaag gatatgttaa tcccggtgat
    15181 gaaacaatgg aggggccatt tggtgatcat acgggatttt actctgctgc agatatctat
    15241 ccggtgtttc atgtaacctg tattacaaca agcgataatc ccgtttatcc tgccaccatc
    15301 gtagggaaac cacccatgga agattgttac atggccaagg caaccgagcg tatattttta
    15361 ccatttttga aaatggtaat tccggagatt gcagatatag agcttcctct tgaaggtgtt
    15421 tttcataact gtgcacttgt atctataaaa aaagagtatc ctgcgcaggc aaagaaggtt
    15481 atcaatgcac tgtggggatt gggtcagatg gcatcaacaa aatatgttgt agtttttgat
    15541 gatgatgtta atctgcgtga ttatagtacg gtggtatgga aacttttaaa caatgttgac
    15601 ccgcgcaggg atctcatgat tgtggagggt ccgctggatg ctcttgacca ttcagcaccg
    15661 tttgctaact ttggcgggaa aatggggatt gatgcaaccc gtaaaacaaa agaagaaggt
    15721 atgggtcgtg actggcctga tgagataaca atgcgtgatg atatcattgc actggttgca
    15781 aagaggtgga aagagtatgg attttaaaaa actgtcgcag cttatattga tagaacaaac
    15841 ggcttttgca ttgccatttg cctatttagg cattttattt gcaggcggcg gaacgctaac
    15901 aacatggata tttgcttcat tagcactggt agcagcacgt acggctggaa tgtgttttaa
    15961 caggctgatt gatgcagaga tagatgcaaa aaatccacgc acaaaggaaa gagcgcttcc
    16021 cagaggtgag gtaaccaaac aggaggtact tatcgtagcg gtcatttcat gtgtggtatt
    16081 tattttttct tcgtatatgc tcaatatgct ttgtttttat ctttcatttt tggcgatagt
    16141 tcttctggta agttattcat actttaaacg gttttcatcg gcatcgcatc tgtatttagg
    16201 cgttgtagag gcggcagctc ctataggagg atatcttgct gcaacaggag agtttacatt
    16261 gatcccgttt atactgggtt gtgccatcat gatgtggatt gcaggtcttg atgttgtgta
    16321 tgcactgtta gatgttgatt ttgacaggca ggaaggattg tattctatcc catcacgata
    16381 tggcgttaaa actgctctta ttatttcatc gttgttgtat cttttatcgt tttcatcatt
    16441 aataattgca ggaatgctta caaacatgaa agaggcttac tggataagtg ttctttgtgt
    16501 tgggataatt tttatttatc agcagatgtt agctcgaaga aaggatatag aacctgcaat
    16561 aaaagaattt tttaaggtaa attcatttat agctcctgtt ctatttctgg gaacatttat
    16621 tgatgtctat tttattgtat aaaaaatata agctctgacc cc
//
LOCUS       DAOSOX010000033        16642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3499_length_16642_cov_8.597998, whole genome shotgun sequence.
ACCESSION   DAOSOX010000033 DAOSOX010000000
VERSION     DAOSOX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_16642_cov_8.597998"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLX22_04070"
     CDS             complement(<1..593)
                     /locus_tag="PLX22_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOT19107.1"
                     /translation="MMGKEHTITSQFIAWGEILLRLSPPGYSRLFQTNMFEAYFGGAE
                     ANCAVTLSQVGIPCRIVSALPDTVLGDAAKRYLQGFGVITDNIVMKQDTRMGLYFYEQ
                     GAGLRASRLIYDRNNSAFAQLQHEDINWNAIIPSNAWFHTTGITLALSNTICDESIKA
                     ASIVNEKKGIVSFDINYRQTLWKSVNDARDAFLRLMPS"
     gene            complement(667..1983)
                     /locus_tag="PLX22_04075"
     CDS             complement(667..1983)
                     /locus_tag="PLX22_04075"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOT19108.1"
                     /translation="MIENPPKGKQLLKVLGPGLVWSASAIGVSELVFATRAGALFGYL
                     LLWAPIVSLIMKYAIFEMAGRYTIATGENVLIAFTRVELDFKLFKLKTGWIIVLFWIV
                     FIASVSGMAGIALSVGSCIYGIFPAISMEVATVCSIVIVGIVLYVGSYTTLENASRIL
                     VLIMVLFLIYALAKTSPDLKEMADGLIPRAPFNTLRELVPLLGWSGAGAIGVVWYSLW
                     VEGSTRGKGNRTIDSDEDKSRIKKWIKINQIDLTINTIITALLTVMFLIAGVLILHPK
                     GMVPQGEKLALQISQIAGENFGRIGEIIFLVGIFGTLFSTLLANIDGLCRVASNSVWY
                     QRRKKDDTKLHYYRLFVIIYVLLTALFSVLFPAPVILLQISAVIDTIFLPLIIGLGIY
                     ICHKHLEAIYRPGKLLTITGVLSVLFFVFFIVLFFYAVIMKVDFSM"
     gene            complement(2047..2748)
                     /locus_tag="PLX22_04080"
     CDS             complement(2047..2748)
                     /locus_tag="PLX22_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19109.1"
                     /translation="MKIEFTLIGQATVCINLDGIVVMTDPWWGSFEFLRAVPLTVDPE
                     SIMPVHYMLVSHNHVDHWCKRAIDVAVKRNIPIIGSTSAVKRAVKHNATKVKAMTPGQ
                     SVECEGFTVIAVPAFHPMAKDAVGFIIKKDSMVLYFSGDTLYTEELKKALTPYTITIA
                     MVQVACSTYPIIGKDGMDLQQAAQFVRECKPTIVIPIHYQVKGKHLVDETLQQWDVEA
                     QKVILPHGKGIAVTV"
     gene            complement(2745..3725)
                     /locus_tag="PLX22_04085"
     CDS             complement(2745..3725)
                     /locus_tag="PLX22_04085"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT19110.1"
                     /translation="MSAICVTGCSGYLGRQVVKTLLEKNFAVTGIDLKDMNEPSVISN
                     KKFTFIRGDIVQKDTVHKACKGVQAIVHCAAALAQFVRDEKDMFAINVTGTKNVVEVC
                     KELKIPQLVFISSVEVYGIDVPVPCSEDVPLQPVCPYGRHKVEGERMCTQLAEKGYGV
                     SIVRPPTINGPGQNEPFLVAQMEAVYKGSPVILPGGGKTKLQMVDVRDVANAIYLCLE
                     KIKKGIIIANVSSDNVPTTRQLVEALFKHAGKKPKIISVPAWIARTIVKGLSAIKLSP
                     IEPQHLEIALRDYVFDNTRAKKLLGWQPEKSDIESTIDAYEYHINMRGWV"
     gene            complement(3743..4447)
                     /locus_tag="PLX22_04090"
     CDS             complement(3743..4447)
                     /locus_tag="PLX22_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOT19111.1"
                     /translation="MAPAYTHEQIIELCKRLEKTYTPAVCDTLDEMGFMHQAMCSGFT
                     PIMPGAAIAGPAFTMEEAKTRKSTRLNEYDPEFVAKVLEDLFGSMQKGQVAVVNVNEF
                     YEAGAWGELMSTTCKYYGGVKGAVVDGPIRDINRVIEVEFPVWARGNIPTDSVGRVDL
                     VGIGGPIWCGGVRVNPGDIIFADCDGVVVIPINDVDLKEVVEKAEEVVTAERRSREEI
                     RGGKSLVEIYKKYGKL"
     gene            complement(4461..7331)
                     /locus_tag="PLX22_04095"
     CDS             complement(4461..7331)
                     /locus_tag="PLX22_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19112.1"
                     /translation="MRYMYSIIFLIILFVSLPLQGYSAIDTFNNSNHDVTITPTPLVY
                     KKGTLLYRRVQVVFPSKETIQKLTIRTSVDGSISTMHTDNPDTSIFLDIKVPDLNTHK
                     AVFTMQCDTTTVKKKITIPAVKPWKIYCVPFTHVDIGFTQSQKNIRIQNYKNIDIELE
                     LVEKTKHYGKDASFKLFTEVSWAVQEYLSDATIEQDKKQKLIKALQKGDFELGAFLIS
                     HQNRFMSPDAIIASLQSTFNLAKKYNVTIHTACIHDVMDFSKVTKILCANDIPYLLVG
                     PNDTRYVLPPLFYGVSPDTSANVLVWHSAGLNGYGENFDLAMRLKLPFDEQAFTTMEN
                     SIALHLAQLEKGYPTAEIKQYFDYGNAHWDYPYDAYLLPYYPAQGGDNQPQNIVPSEI
                     AREWNKKMINPQIIIATPSQFFDYVRSRYNSVIPRIKGEMAPFWGEQIYLDFIQADPQ
                     RLLISYMFDRGMYQLASTVVDAMVHDKPINDSFHSIAESAYKAILLNNDHNPRPVPFG
                     KTHYTSDDVKDWMNTRSEWVKLPQILMHKAGYSEAFHDGRSWEKIKYKKNIIIIDSHF
                     YRVTIDQKKGRITGVYDKELQKEWIRNNAQYGFNQYVQTVRGENGARRDYYQCIEGFK
                     SPDVLVYRYHDDYRLIIEGTVKEYYKGMELIADFLDKSFHVSLPPWLLKVVYFFYQLF
                     TPSLTLTQEIVLPADEKKIELIQTFSGSQPQIAEHCFAYPLTSDALYYDAAFTPLLWE
                     SIYNGGNIIPAAKNTAPFKSINDTLYPFQWMYGLPPSFHCDAFTMVRDDAMYAVFMTK
                     DSKAIVPESSYSRDSNGFYHCCIGWTLWGKVGLGQSLKDKTVFRSTFTTFSADSITYA
                     LAKAYDKSFQEYGYTTSSPVTVSNPAIAVVWMYPITNKKFTVGLFETAGQSQSTHISI
                     KSIQPIAVYYSTCYGERKGDVADKQLLSLKPHELRFVTIEIQ"
     gene            complement(7352..8053)
                     /locus_tag="PLX22_04100"
     CDS             complement(7352..8053)
                     /locus_tag="PLX22_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOT19113.1"
                     /translation="MDALQPIKRVKLSESIVDMIIEKIKDGGFAPGTKLPPERDLMVQ
                     LGVGRSTLREAIQSLALMGILDVRPGEGTFIRLIRREDIISPHILAPLVDETSIGDFF
                     EARLLVEPKIASIAAKRRDEKDIARIQETLTTIDALIHKPDEVDRWSGEFHLRVAYAS
                     KNMVCARFVEAILSFLIGRRSNADINREFLQWEYDSHTIIFQSIKDGDEKKAYQNMHR
                     HIEDVVIWYKKLGII"
     gene            complement(8517..12227)
                     /locus_tag="PLX22_04105"
     CDS             complement(8517..12227)
                     /locus_tag="PLX22_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOT19114.1"
                     /translation="MEKIFKRDFKYREIPYNYTSFSDKEIIVKYFDAHTWDLVEALRE
                     KRRTGRSAKLLFENIGDIFIIDRNPYIQFDIIENPTKLKNLQKRHQRRLATVKEGANG
                     DGQVLELIDKIEKLDDTAFKKLKECKKQQEKILNKLKHITSEKNIQFSPFHRAAHVTD
                     ATDWRVEYPFVVVYPDTVEEVQELVKAAKKLGLVIIARGGGTGLTGGAIPLLPNTMVI
                     NTEKFNRIGRIEKTYLNGNEVPVITVDAGAVTEDVIQYCEHQNYIFATDPTSAWACTI
                     GGNIAENAGGKKCVMWGTCIDNVLSFDMVDHNGELITVKRVKHLYRKIFPNDEVEFEV
                     IKNNKVVKTITLSGLDIRKKGLGKDITNKALGGLPGLQKEGCDGIIVSATFVLYKPFK
                     FTRTVCFEFFGNNMINASKAIVEIVKTFENDPVVFLTALEHFDEQYVKAIQYKNKSLR
                     TEIPKAVLVVDIESDEEEHLEKATLDLVAKVKQYNTEGIIAKDAGMREKFWQDRKNLS
                     AIARHTNAFKLNEDVVIPLERLQDFSDFIEKLNIQKELENNIQVIAVLADYLQERIKT
                     EDDKLCLERCQSGVAQLKTMQSRYMDILDNIDTHAKDYFKYDSEYALRMDSVFQLLQN
                     NELSINFDADVIEPLSKLFYGYDDILNTIYQLQADTQKRRIIVATHMHAGDGNIHVNI
                     PVHSNDYLMMKDAEETAANVMQATVTFGGVVSGEHGIGLTKIRFIDDDTLAKFAEYNR
                     LADPEDLFNPLKLTRDFNLETVYTPSFNLLEGEAFILKATDLETVFNSIATCIRCGKC
                     KSVCNTHYPDGMVFYNPRNKILATALVMEAVIYDIQTSTSLSFKHFTNLREISNYCTI
                     CHNCQKPCPVSIDFGAITLKIRSILEARRKSTFKPVTSFTLFYLKQKGYYINKLFRVL
                     LLRWVYSLQRFGYYIVKPIPHVVQFISPYIAMMLKGRLPKSGHKSLRDLLQLKNTNTF
                     YVFRNKNKPVIKTVVYFPGCGSERMFPDISIATVALLYYAGVRVIIPPTYLCCGYPMR
                     ANGKLNQARIKTNENRVLFHRMADTFSYLDIGDIVVSCGTCYEMLLDYQLENVFRGAR
                     LIDVNEFIAREGLYSKTINETLIYHEPCHTPLKAMGYQKTFSLLLNTKPLSVPNCCGE
                     GGTLALSTPDISNSLRERKETNIRANVVRKNVMVLTTCPSCVQGLCKIQDTIKVTGKS
                     LVVYIAEQCLGKEWKRQFLKEIKAQCFDRYIY"
     gene            12390..12989
                     /gene="yedF"
                     /locus_tag="PLX22_04110"
     CDS             12390..12989
                     /gene="yedF"
                     /locus_tag="PLX22_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOT19115.1"
                     /translation="MKTIDARGLECPQPVLLTKSAIEDENPDELEVLVSSQTSAGNVT
                     RFAEHSGYRVQTSIKDDYFTIVLHKAIEAKPFASAKLQSSMNNGKNVVVFITSNEFGR
                     GDTELGQLLMKSFINTMNEANVCPTTIIFAHSGIFLTTEDSPVIQPLQQIEKKGVQIL
                     NCGTCLEYYKRTKLLKVGTISNMYTITETLMMADKLIKL"
     gene            complement(12991..14034)
                     /gene="ruvB"
                     /locus_tag="PLX22_04115"
     CDS             complement(12991..14034)
                     /gene="ruvB"
                     /locus_tag="PLX22_04115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT19116.1"
                     /translation="MKKKEENNNPGIISPLSLDEEDFEKKLRPQRLSEFIGQKQLTEN
                     LRVFIESAKLRKKPLDHVLLAGPPGLGKTTLAFIVANELGVNIKATSAPAIEKSGDMA
                     SLLTNLEAMDVLFIDEIHRLSPAIEELLYSAMEDGELDIILGQGVGAKSIKLQLAPFT
                     LIGATTRTGLLTSALRDRFGIPLRLDYYEIDDLETIALRTAAIIGCSIEEEAAQEIAR
                     RSRRTPRIVNRLVKRVLDFAIVHKKDMIDKQIALYALDKLHIDMLGLDDMDRKILSTI
                     IDKYDGGPVGIKTIAISIGEEITTLEDFYEPYLVQIGMLKRTPRGRVAARLAYEHLGL
                     AYKGIEDIQTLFE"
     gene            complement(14012..14494)
                     /locus_tag="PLX22_04120"
     CDS             complement(14012..14494)
                     /locus_tag="PLX22_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT19117.1"
                     /translation="MIIMILTDYLNKKNIIINPSVKDRWELIEAMLDMAIKNKSLKAE
                     HRDAIKKTLFEREKSMSTGIGKGVAIPHCSCPYVDDVVMIMSLSQKGINFDSIDNLPV
                     HIVILLIVPKDKISQHIKTLANIAKLMNDDSLRESLIGAKDVQDIIKILKEYEKKGRK
                     "
     gene            complement(15331..15951)
                     /locus_tag="PLX22_04125"
     CDS             complement(15331..15951)
                     /locus_tag="PLX22_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT19118.1"
                     /translation="MKKAATVLLFMAGLLIYAGCKAQQAEPQTVVNLKAAITGETTAS
                     ATYAAFAIKAKEENLAPIAVLFDAASRAEKIHANNHTRALAKLGKKMDAINPSVTVKS
                     TRENLETAIKGENYEVVSMYPDFIKKATDENVAEVLTTFKYAFEVEKIHYKLYTDALA
                     SLDKKKVAGMPVLYAVCPICGNTMSGNMPAVCGICGAPQNTFIIVK"
BASE COUNT         4795 a         3810 c         3032 g         5005 t
ORIGIN      
        1 gagggcatca gccgtaaaaa tgcatccctg gcatcattaa cacttttcca tagtgtctgg
       61 cgatagttaa tatcaaaaga aactattccc tttttttcat taacaatact tgctgcttta
      121 atgctttcgt cacagatagt atttgaaagc gccagggtta tacctgttgt atggaaccat
      181 gcattgcttg ggattattgc attccaatta atgtcttcat gctgtaattg tgcaaatgca
      241 ctgttgtttc tgtcatagat aagccgtgaa gccctgagtc cggcaccctg ttcataaaaa
      301 tacaacccca tgcgcgtatc ctgtttcatc acaatgttgt cagtaatgac gccaaacccc
      361 tgtaaataac gctttgcagc atcacccaga acggtatcag gcagagcaga aacaatcctg
      421 catggtattc ccacctgtga aagtgtcacc gcacagtttg cctctgctcc tccaaagtac
      481 gcttcaaaca tatttgtctg aaaaagcctg ctgtagcctg gaggtgataa tcgcagcagt
      541 atctcacccc aggcaataaa ttgacttgtg atagtatgct cttttcccat catccagcac
      601 catcatcagt aactatcgcc cacaacaatt ttttgcccta tcatccatgg gtaatagtgt
      661 acatggttac attgaaaaat ctaccttcat tatcaccgca tagaaaaata gtacaataaa
      721 aaaaacaaaa aatagcaccg acagcacccc tgttatggta agcagctttc ccgggcggta
      781 tatagcttcc agatgcttat ggcagatata gataccaagc cctatgatta acggtaaaaa
      841 tatagtatca atcaccgctg agatttgtaa aagaataacc ggcgccggaa ataataccga
      901 gaaaagagct gttaacagta catatatgat tacaaacaga cggtaatagt gtaactttgt
      961 atcatccttt ttacgacgct gataccatac ggaatttgaa gcaacacggc atagcccatc
     1021 aatgtttgca agcagggtgc tgaacagcgt accaaaaatc cccactaaaa agattatctc
     1081 ccctatgcgt ccaaagtttt cgccggcaat ctgtgatatc tgaagagcaa gcttttcacc
     1141 ctgcggcacc attcctttgg gatgtaatat caacacaccc gcaattaaaa acatcacggt
     1201 taacagcgca gtgataatgg tgttgatagt taaatctatc tgattaattt ttatccattt
     1261 tttgatcctg cttttatctt catcactgtc tatcgtccta ttgccctttc cccttgtgct
     1321 tccttcaacc catagcgaat accacacaac accaatggca ccggcacccg accatcctaa
     1381 aagcggtaca agctcacgca gggtgttaaa tggagctcgg gggattaacc cgtctgccat
     1441 ttctttcaga tcgggcgatg tttttgccag agcatatatc aaaaacaaaa ccattatcaa
     1501 cactaaaatt cttgatgcat tttccagggt agtgtagctt cccacataca atactatgcc
     1561 aacaataacg attgagcaaa ctgtagctac ctccatggaa atagcaggga atataccata
     1621 aatacagctt cccacggaca gcgcaatccc cgccataccc gaaacgcttg caataaacac
     1681 tatccagaat aatacaatga tccatcctgt ttttaattta aaaagtttaa aatctaattc
     1741 tacccgggta aacgcaataa gcacattttc accggttgcg atggtatacc gtccagccat
     1801 ttcaaatatg gcatatttca taatcaaaga aacaataggt gcccacagca ataaataccc
     1861 aaaaagtgcc cctgcacgag tggcaaaaac aagctcagac accccgatgg cgcttgccga
     1921 ccatacaagc cccggaccta aaacctttag taattgtttc ccttttggtg gattctctat
     1981 catggtagta tcccccctta taacgttttt taaagacgca gagagtatag tgttccctgc
     2041 ctttgcctat accgtaaccg ctattccttt tccatggggc aatataactt tttgcgcttc
     2101 cacatcccat tgttgcaacg tttcatcaac aagatgctta cccttaacct gataatggat
     2161 ggggatcaca atggttggct tgcattccct cacaaactgc gctgcctgct gaaggtccat
     2221 cccatctttg ccaatgatag gataggtaga gcatgccacc tgcaccatgg cgatagttat
     2281 tgtatagggg gtcagagcct tttttaactc ttccgtgtac agggtatccc ctgaaaaata
     2341 cagcaccatg ctatcctttt tgataatgaa tccaacggca tcctttgcca tagggtgaaa
     2401 ggctggaaca gcaatgacgg taaatccttc acactcaaca gactgccctg gtgtcatagc
     2461 ctttaccttt gttgcgttat gttttacagc ccttttgact gcactcgttg aacctatgat
     2521 tggtatattt cgtttaactg caacatcgat agcacgctta caccagtggt caacatggtt
     2581 gtgcgaaacc agcatataat gaacaggcat tattgattcg ggatcaacgg ttaagggaac
     2641 tgcccgtaaa aattcaaagg aaccccacca tggatcggtc ataaccacga tgccatcaag
     2701 gttaatacat accgttgcct gcccaataag ggtaaattca atcttcatac ccacccccgc
     2761 atgttaatat ggtactcata cgcgtcgatg gtactttcaa tatcactttt ttcgggctgc
     2821 cagcctaata atttttttgc acgtgtatta tcaaagacat agtcgcgtaa tgctatttct
     2881 agatgttgcg gttcaatggg cgatagtttt atggcagata atccctttac aatagtacgg
     2941 gcaatccatg caggaactga aattattttt ggctttttgc cggcatgttt aaagagcgcc
     3001 tctacaagct gtcgcgttgt tggcacatta tcactggaaa catttgcaat gattatacct
     3061 ttttttattt tttcaagaca caaataaatt gcatttgcaa catcgcgtac atccaccatc
     3121 tgtaattttg ttttaccacc acctggcagt ataaccggac ttcctttgta caccgcttcc
     3181 atctgggcaa ctaaaaatgg ctcattctgc ccgggaccgt tgatcgttgg tgggcgcact
     3241 attgaaacac catatccttt ctctgccagc tgcgtgcaca tacgctcccc ctcaaccttg
     3301 tgcctgccgt aagggcatac aggctgtaac ggaacatctt cactgcaggg gaccgggaca
     3361 tcaatgccat acacctctac cgatgagata aagacaagtt gcggaatttt taattctttg
     3421 catacctcta caacattttt tgttcccgta acattaatgg caaacatgtc cttttcatcg
     3481 cgcacaaact gcgctaaagc cgccgcacaa tggacaatag cctgtacacc cttgcatgcc
     3541 ttgtgcacag tatctttctg tacaatatca cccctaataa aggtaaactt tttattggaa
     3601 ataactgatg gttcattcat gtctttcagg tctatccctg ttacagcaaa gtttttctct
     3661 aacagggttt ttacaacctg tcgtcctaaa tagcccgaac atcctgttac gcatatcgca
     3721 ctcatggctc cctccagata atttatagtt taccgtattt tttatatatc tccaccagag
     3781 attttccccc tcgtatctct tctcgtgaac gccgttctgc tgttacaacc tcttctgcct
     3841 tttccaccac ctctttcaaa tcaacatcgt taatggggat taccaccacg ccgtcacaat
     3901 cagcaaatat aatatcaccc ggattaaccc tgacaccacc acaccagatg gggcctccaa
     3961 tacctacaag gtcaaccctc ccaacagaat cagtaggtat attgccacgt gcccatactg
     4021 gaaattctac ctctataacc ctgttaatat cacggatggg accatccacc accgctcctt
     4081 tcacaccacc ataatacttg caggttgttg acataagctc accccatgca cctgcttcat
     4141 aaaactcgtt aacattgacc actgccacct gacctttttg catgcttcca aaaagatctt
     4201 ccagaacctt ggctacaaac tcagggtcat actcattgag gcgggtgctt ttgcgggttt
     4261 ttgcttcttc catggtgaaa gcaggaccgg caatagcagc ccctggcatg ataggagtaa
     4321 atcctgagca catcgcctga tgcataaatc ccatctcatc aagcgtatca catactgcag
     4381 gcgtataggt tttctccaac cttttacaca gttcaattat ttgttcatgg gtatacgctg
     4441 gtgccatata aagcctcctt ttattgaatt tcaatagtaa caaaacgtaa ttcatgtggc
     4501 ttaagcgata gtaactgttt atctgccaca tcacctttcc tttccccata gcaggtagaa
     4561 taataaactg ctataggctg tatggatttg atacttatgt gcgtactctg tgattgtccg
     4621 gcagtttcaa atagtccaac ggtaaacttt ttattggtta tggggtacat ccacaccaca
     4681 gcaattgctg gattggacac tgttaccggt gaagaggttg tataaccata ttcctggaaa
     4741 cttttatcat acgcctttgc cagtgcatag gtgatgctat cagcgctaaa agttgtaaag
     4801 gtactcctga atactgtttt atccttcagg ctctgcccaa gtcctacttt tccccataat
     4861 gtccatccaa tgcagcaatg ataaaatcca ttactatccc tggaataact tgactcagga
     4921 actatcgctt tgctgtcctt tgtcataaat acagcataca ttgcatcatc ccttaccatg
     4981 gtaaacgcat cacagtggaa cgatggcggc aatccataca tccactggaa gggatataaa
     5041 gtgtcattga tacttttaaa cggtgcagta ttctttgcag caggaatgat attgccacca
     5101 ttataaatgg attcccagag taatggagta aatgcagcat catagtacag tgcatccgat
     5161 gttaaaggat atgcaaaaca atgttcagct atctgtggct gtgacccgct gaatgtctgt
     5221 atcaattcaa tctttttttc atccgctgga agtacaatct cctgggtaag ggtaagcgaa
     5281 ggtgtaaaaa gctggtaaaa aaaatacacc accttcaaca gccatggcgg caatgaaaca
     5341 tgaaatgatt tatccagaaa atctgcaatc agttccatcc ccttgtaata ctctttaacc
     5401 gtaccctcaa tgataagtct ataatcatca tgataacggt aaaccaaaac atcaggactt
     5461 ttaaatcctt caatgcattg ataataatcc cgccgtgcac cattttcgcc ccgcactgtc
     5521 tgtacatact gattaaatcc gtactgagca ttgtttcgta tccactcctt ttgcagctct
     5581 ttatcatata caccggtaat gcgtcctttt ttctggtcga tagttaccct gtaaaaatga
     5641 ctatcgataa taattatatt ttttttatac tttatttttt cccagctccg cccgtcatgg
     5701 aaagcctcac tatatccagc cttatgcatc aatatctgtg gcagctttac ccattcggag
     5761 cgggtattca tccagtcctt aacatcatcc gaggtataat gtgttttacc aaaaggaacg
     5821 ggacggggat tgtgatcatt attgaggagt attgctttgt atgccgattc agcaatacta
     5881 tggaaagaat cattaatggg cttatcatgc accatagcat caacaacagt tgaagcaagc
     5941 tgatacattc ctctatcaaa catgtagctt atcaaaagtc tttgggggtc cgcctgaata
     6001 aagtccagat atatctgctc gccccagaat ggtgccatct cacccttgat gcgcggtatt
     6061 acactgttgt agcgcgaacg cacataatca aaaaactgcg aaggtgtagc aatgataatc
     6121 tgtggattta tcattttttt attccattcc cgggctattt cagaggggac gatgttttgt
     6181 ggttgattgt cgccaccctg tgcaggatag taaggcaaaa ggtatgcatc gtagggatag
     6241 tcccaatgtg cattgccata atcaaaatac tgctttattt cagcggtagg gtaccctttc
     6301 tcaagttgtg ccagatgcag ggcaatgctg ttctccatag tggtaaatgc ttgctcatca
     6361 aagggcagtt ttagacgcat agcaaggtca aagttttcgc catacccatt aagtccggca
     6421 ctgtgccata ccagtacatt cgcactggta tccggcgaaa caccataaaa cagcggaggg
     6481 agtacatatc gtgtatcatt tgggccaacc agaagatagg ggatgtcatt tgcacacagt
     6541 atcttggtta cctttgaaaa atccatgaca tcatgaatac acgcagtatg tattgttaca
     6601 ttgtactttt ttgcaaggtt aaaggttgat tgcagtgaag ctatgattgc gtcagggctc
     6661 ataaatcgat tctggtgtga aataaggaat gctcccagct caaaatcgcc cttttgtaat
     6721 gctttgataa gcttttgctt tttatcctgt tcaatggtag catccgacaa atattcctga
     6781 accgcccagc taacctcggt aaaaagctta aatgatgcat ccttaccata gtgttttgtc
     6841 ttctctacca gttccagttc aatatcaatg tttttgtaat tttgtatacg aatattcttc
     6901 tgcgattggg taaacccaat atcaacatgg gtaaatggaa cgcagtatat cttccatggt
     6961 ttaacagcag ggattgtaat cttttttttg acagtggtag tatcgcactg catggtaaaa
     7021 acagctttgt gcgtgtttaa atcaggcacc ttgatgtcta aaaagatact ggtatccggg
     7081 ttatctgtat gcattgtgct gatagaacca tcaactgatg tccgtatggt cagcttttga
     7141 atagtttctt tactgggaaa tacaacctgt acacggcggt agagcagtgt acccttttta
     7201 tataccagcg gtgttggagt aatagttaca tcgtgattgc tattattaaa tgtatcaatt
     7261 gctgaatacc cttgaagtgg taacgataca aaaagtatta ttaaaaaaat aatgctatac
     7321 atgtatcgca tggcgccctc caaaaaaatt attaaataat acccagtttt ttataccata
     7381 tcacaacatc ctcgatatgc ctgtgcatat tctggtacgc tttcttttca tcaccatctt
     7441 taattgattg aaatatgata gtatgtgaat catattccca ttgcaaaaat tcacgattga
     7501 tatcagcatt ggaacgtctc ccaatcagaa aggaaagaat tgcctcaaca aatcgggcac
     7561 ataccatatt ctttgatgca taggcaacgc gcagatgaaa ctcccctgac catctatcca
     7621 cctcgtcggg tttgtgtatc aacgcatcta ttgtagttaa tgtttcctgt atcctggcaa
     7681 tatccttctc atcacgccgt tttgctgcta ttgatgctat tttcggttct accagtaagc
     7741 gtgcttcaaa aaaatcacca atgctggtct catcaacaag gggtgctaaa atgtgtgggc
     7801 ttatgatatc ctcacgccgt atcaagcgta taaaggtacc ttctccgggg cgtacatcaa
     7861 ggatacccat aagcgctaat gactggattg cttcacgcag ggtagagcgt ccaacaccca
     7921 gctgaaccat taaatccctt tccggcggca gttttgtccc tggcgcaaac cctccatcct
     7981 tgatcttttc aataatcata tccacgatag attccgagag cttcacccgc ttgataggtt
     8041 gtaatgcatc catattacct ctgatattct gatggtctga tgataagata taataatagc
     8101 cagtatttgt caagaaaata ttacaatttt tgcaccgata atcatgaggg catggtagat
     8161 attcgttctg gtaaatgtga atagcattca aaaatagatc gctatgtatg gcttataccg
     8221 ttagattgaa ccaatttaat taatcggttg gtcaaagatc aactgggata tcgctggaca
     8281 gtggtgtatg atctggagaa tgaccccggt gagacatata ctgttatcga tacgtatcca
     8341 gatatcgcac acaacctgta aaaaaaaatg ggagcgctgg aaagctgaaa ccagcaaaaa
     8401 tcctcgatat tttttcatta aatctatttg agaataccag caaagaccac aagcatgatt
     8461 gtattaccag ctaaaaggtt gccaatgaca tttttttgca ctgctggttt ccagtttcaa
     8521 taaatgtacc tgtcaaagca ctgtgctttt atttctttca gaaactgtct cttccactct
     8581 ttaccaagac attgttcagc tatatataca acaagcgatt taccggtaac ctttattgta
     8641 tcctgaatct tacataatcc ctggacacag cttggacagg ttgtgagcac catgacattt
     8701 ttcctgacaa catttgccct tatattggtt tctttccgtt cacgtaaact gtttgaaata
     8761 tcaggtgtgg ataatgctaa tgtcccccct tcaccacagc aattaggaac cgatagtggc
     8821 tttgtattga gcaacaggga aaatgttttt tgatatccca tagcttttaa cggtgtatga
     8881 cacggttcat gatatatcag tgtctcattg atggtttttg aatataatcc ttcacgggct
     8941 ataaactcat tgacgtcaat gagccttgct cctctgaaaa cattctctaa ttgataatcc
     9001 agaagcatct cataacaggt accgcaggat actacaatat ctcctatatc cagataggaa
     9061 aaagtgtctg ccattctgtg gaaaaggacc ctattctcat tggtttttat ccgtgcctga
     9121 ttcaatttac catttgctct cattggatag ccgcagcaca gatatgttgg cggaataata
     9181 acccttactc ctgcataata cagcagagca acagttgcaa tactaatatc agggaacatc
     9241 cgttctgaac cacagccagg aaaatatacc acggttttta taactggctt attcttattc
     9301 ctgaacacat aaaatgtatt tgtatttttt aactgtaaca gatcgcgcaa agacttatgc
     9361 cctgacttag gcaatctccc tttcagcatc atagcgatat agggcgaaat aaactgcacg
     9421 acatgaggga taggtttgac gatataatat ccaaaccgct gtaaagaata cacccatcgt
     9481 agcagcagca ccctgaatag tttatttata tagtaccctt tttgtttcag atagaagagc
     9541 gtaaatgatg ttacgggctt gaatgttgac tttcttcgag cctccagaat tgatcgtatc
     9601 tttaaggtaa tggctccaaa gtcaatagag accggacatg gtttttgaca gttatgacag
     9661 atggtacaat aatttgaaat ctcacgtaaa ttagtaaaat gcttaaaact caagcttgtg
     9721 gatgtctgta tatcatagat tacagcctcc ataacgagag ctgtcgccaa aattttgttc
     9781 cttggattgt aaaataccat accatcgggg taatgggtgt tgcataccga tttacattta
     9841 ccacatcgaa tgcaggtagc aatagagtta aaaactgttt ctaaatcggt tgcttttaaa
     9901 ataaatgctt caccctcaag aagattaaag gaaggtgtat agacagtttc cagattaaaa
     9961 tccctggtta attttaaggg attaaaaaga tcttcaggat cagctaatct gttgtactca
    10021 gcaaattttg ccaatgtatc atcatcaata aatcgtatct ttgtaagccc tatgccatgt
    10081 tcaccgctta ccactccgcc aaatgtaaca gtagcttgca taacattggc tgcagtctct
    10141 tcagcatctt tcatcataag ataatcattt gaatgaacag gaatattgac atggatatta
    10201 ccatcaccag catgcatatg agttgctact atgatacgac gcttttgtgt atcagcttgt
    10261 aactggtaaa ttgtgtttaa aatatcatca tatccataga aaagttttga cagtggttca
    10321 ataacatcag catcaaaatt aatgcttaat tcattattct ggagtaactg aaaaacagaa
    10381 tccattctca gagcatattc tgaatcatat ttaaaataat cttttgcatg cgtatcaata
    10441 ttatccagta tatccatgta ccgggactgc atcgttttta attgagcaac ccctgactgg
    10501 catcgttcca gacatagttt gtcatcttcg gttttaatac gttcctgcag gtaatctgca
    10561 agaacagcta tcacctgaat attgttctct aattcttttt gaatattaag cttttcaata
    10621 aaatctgaga agtcctgcaa ccgttccagt gggataacga catcttcatt caatttaaat
    10681 gcattggtat gtctggcaat ggcgcttaaa ttttttctat cctgccaaaa tttttcacgc
    10741 atccctgcat cttttgcaat tattccttca gtattgtact gtttaacctt tgcaactaaa
    10801 tctaaggtag ccttttctaa atgttcttct tcatcgcttt caatatctac cacaagtacc
    10861 gctttgggga tttctgtacg aagcgattta tttttatact gaattgcctt aacatactgc
    10921 tcatcaaaat gttcaagcgc tgttaaaaac acaacaggat cattttcaaa ggttttaaca
    10981 atctcaacaa ttgctttcga cgcattaatc atgttgttgc caaaaaactc aaagcatacc
    11041 gtccgggtaa atttaaaagg cttatacaaa acaaaggtag ccgaaacaat aataccatca
    11101 catccctctt tttgtaatcc tggcaagccg cccagtgctt tattggttat atccttgccc
    11161 aatccttttt ttcgtatatc aagtccggaa agtgttatgg ttttgactac tttattattt
    11221 tttataacct caaactcaac ctcatcattg ggaaatattt ttctatacag gtgtttaacc
    11281 ctttttacgg taataagttc tccattgtgg tcaaccatat caaacgacag cacattatca
    11341 atacaggttc cccacataac acatttttta ccgccagcat tttcagcaat gttcccgcca
    11401 atggtacatg cccatgcaga agttggatca gttgcaaaga tataattttg atgttcacag
    11461 tattgtatta catcctcggt aactgcaccg gcatcaactg ttataacagg aacttcgtta
    11521 ccgttcaggt atgtcttttc aatcctgcca atacggttaa atttttcagt attgatgacc
    11581 atcgtattgg gaagaagggg gatagcaccg ccggtaagcc ccgtccctcc tccacgagca
    11641 ataatgacaa gccctaactt ttttgcagcc tttaccagtt cctgtacttc ttctactgta
    11701 tcagggtaaa caacaacaaa gggatattca acccgccaat cagtagcatc ggtaacatga
    11761 gcagctcggt gaaaaggact aaattgtata tttttttccg atgtaatatg ctttaattta
    11821 ttcaatattt tttcttgctg ctttttacat tcttttaatt ttttaaaagc agtatcatcc
    11881 aatttttcaa tcttatcaat caattcaaga acctgcccat ctccattagc accttctttg
    11941 actgtcgcaa gccttcgctg atgtcgcttt tgcagatttt ttaatttcgt gggattttca
    12001 ataatatcaa actgaatata cgggtttcta tcgataataa agatgtctcc aatgttttca
    12061 aacagcaatt ttgcacttct gcctgtacgt cgtttttccc gtagtgcttc cactaaatcc
    12121 catgtatggg catcaaaata ttttactata atctctttgt ctgaaaatga ggtatagtta
    12181 taaggtattt ctctatattt aaaatctcgt ttaaaaatct tttccattac aattcctgta
    12241 aaaaaacgtt tgtaaacaaa acattaatta ataattgtat cacaattaca atgatttaca
    12301 atattggagc gatagttatt gtttatatca tatttccgta tgtattggtc aatagcaaaa
    12361 ttaaaaattt ttttaaaagg agcctttata tgaaaacgat agatgctcga ggactggaat
    12421 gcccacagcc ggtactattg acaaaatctg caatagagga tgaaaaccct gacgaactgg
    12481 aagtgttagt aagcagccaa acatcagcgg gcaatgttac ccgttttgcc gaacatagtg
    12541 gataccgtgt acaaaccagt ataaaagatg attacttcac tattgtattg cataaagcaa
    12601 tagaagccaa accgtttgct tcagcaaaac tacaatcatc aatgaataat gggaaaaatg
    12661 ttgttgtttt tataaccagc aatgaatttg gcaggggcga tactgaacta ggccagctcc
    12721 tcatgaaatc attcatcaac accatgaacg aagccaatgt gtgcccaaca accataatct
    12781 ttgctcactc gggcatattt ttaacaaccg aggattcacc ggttattcaa ccactccagc
    12841 agattgaaaa aaaaggggta cagatactca attgtggtac ctgccttgag tattataaac
    12901 gcacaaaact gcttaaagtg ggaactatct caaatatgta taccattact gaaacattaa
    12961 tgatggcaga taagcttata aagctataaa ctattcaaag agtgtttgta tatcctctat
    13021 ccctttatag gcaagcccta aatgttcata cgcaaggcgt gctgcaaccc ttcctcgcgg
    13081 ggtacgtttg agcatcccaa tctgcaccag atatggttca taaaaatctt ccagcgttgt
    13141 aatctcttca cctattgata tggctatcgt ctttatcccc accgggcctc cgtcatattt
    13201 atcaataatg gtggacaata tctttctgtc catatcatcc aatcccagca tatcaatatg
    13261 aagtttatcc agagcataca gggcaatttg tttatcaatc atatcctttt tatgaactat
    13321 cgcaaaatca agcacccgct taacaaggcg attgactatg cgtggtgtcc gacgcgaacg
    13381 ccttgcaatc tcctgtgcag cttcttcttc tatgctacac cctattattg ctgcggttcg
    13441 taaagcaata gtttccagat catctatctc gtaataatca agacgcagcg gtataccaaa
    13501 cctatcacgc aacgctgagg ttaaaagacc tgtgcgcgtt gttgcgccaa tcagggtaaa
    13561 gggggcaagt tgcaatttta tgctctttgc accaacaccc tgccctaaaa taatatcaag
    13621 ctcaccatcc tccatagcgc tatacaaaag ctcttcaatc gctggcgaaa gcctatgaat
    13681 ttcatcaata aaaagcacat ccattgcttc cagattggtt aaaagtgaag ccatatcgcc
    13741 agacttttct attgccggtg ccgaagtagc tttaatatta acgcccagct cattggcaac
    13801 aatgaatgcc agtgtggttt tgcccagtcc tggaggccct gcaagcaaaa catggtccag
    13861 tggctttttc cgtaattttg ccgattcaat aaatacacgt aagttttcgg ttaactgctt
    13921 ctgtcctata aactcactga gacgttgagg gcgtaatttt ttctcaaaat cttcttcatc
    13981 cagtgataat ggtgagataa tgcccgggtt attattttct tccttttttt tcatactctt
    14041 ttaaaatctt tataatgtcc tgaacatcct tagcaccaat gagcgattca cgtaaactat
    14101 catcattcat gagcttggca atattagcca gtgttttgat atgttgtgat attttatcct
    14161 tgggaacaat aagtaaaata acaatgtgaa ccggtagatt atctatagaa tcaaaattaa
    14221 tgcctttttg gctcaatgac atgatcataa caacatcatc gacataggga cagctacaat
    14281 gcggaattgc aacccccttg ccaatcccgg tactcatgga cttttcgcgt tcaaacaatg
    14341 ttttttttat tgcatcacga tgctctgctt tcagcgattt attcttgatt gccatgtcga
    14401 gcattgcctc aattaattcc catctatcct taacagatgg attgataata atattctttt
    14461 tatttaaata atctgttaaa atcataataa tcattggtcc tgcaaaaaaa atacacgcat
    14521 acagcaatct aaactaagat actacctgtg caagattttc gtcaagtgtt atatggtttt
    14581 ttactgtata tattttagag atagattttt acggaagttg ctcatggtac actaaagtac
    14641 acaaaccatt gagatcttct gttttgtata gatcatgaga tttaaaaaaa cagatagacc
    14701 tcaaaaattc tcattttcca ttgaaacgtt atgccgcaga atctccacca tagcagcgta
    14761 tccatcttcc agtactatag gagatgcctt ctcgattcag caacctcccc gaggtccgtt
    14821 tcatactgta gttcctcgaa cgccatcatt gccatccagc catgagcggt gcagcatcat
    14881 ggttttaggc tttgtttcct ccgcgcgaac atcagcatgc ggtgcatatc aatcctccgg
    14941 ccgcggaatc gttccacaat gcagatggat gatatggccg acctcagttg atcacggcga
    15001 aggcccgagt acaattataa aattgcatca tacacgattg ctgtggtact ttcaggacga
    15061 tgcggatccc cgcatgacac cgcgtacact cctacaaaaa ttacggtaca cgatgtgcgc
    15121 agaatatcat atgcttaaaa aatacggtct ctgtgatacc tgcatcatca tacgcccttg
    15181 aaaatatttg attaaatgaa agaatacata tttcccggcc ggaggacagc acgccatggc
    15241 tgcgccgttc gcaccaccta aactttccgc ccccgcgggg ccgctttata tatcacggtc
    15301 ccttgctgtt catgcttcgg caacaggaat ctacttcact atgatgaatg tgttttgtgg
    15361 ggcgccgcag ataccgcaca cggccggcat gttcccgctc atggtgttcc cgcaaatcgg
    15421 gcacacggcg tagagcaccg gcatccccgc cactttcttc ttatccagac tggcgagagc
    15481 atcggtgtaa agcttgtagt ggatcttctc cacttcgaac gcgtatttaa acgtggtcag
    15541 gacttccgcg acgttctcat cggtcgcttt cttgatgaaa tccgggtaca tgctgacgac
    15601 ctcgtaattc tcgcctttta tcgcagtctc gaggttctct cgcgtggact tcaccgtcac
    15661 cgaggggttg atcgcgtcca tcttcttccc gagcttcgca agagcgcggg tatggttgtt
    15721 ggcgtgtatc ttctcggccc tggacgccgc atcgaaaagc accgcgatgg gggctaaatt
    15781 ttcctctttc gcctttatcg cgaaagccgc gtaggtggcg ctcgccgtcg tctcacccgt
    15841 tatcgcggcc ttcaggttca ccaccgtctg gggctcagcc tgctgcgcct tgcacccggc
    15901 atagatcaac aatcctgcca taaacaacaa tactgtcgct gcctttttca tgcttccctc
    15961 ccatgatacc atcacgcaga atattccagc tcctggaatc ggaaatcacc gcaccacgag
    16021 caaacgcaat gcccctgctg acggcgagat gtccaaattc cgagcgtgga attgattgac
    16081 ctcattcaat aatctatcac ccatcatgtc aagtaaaaag ctctttccca atccgggggg
    16141 taaaataata gttcttcgtg catgtacgtc aatattttca acctgagata ttgctcatct
    16201 caaataccgt aagctctccg ttgtaacact cggatttcat cgcccaaccc atcgtcaaaa
    16261 cccaaccgaa ccttgttgaa gaatcacaat atggtggaat gccatttcaa taatagagta
    16321 cgtcccttat atgttatgct ggatatataa atagtatttc tgagtaatag cgaagaatct
    16381 tgctgtttcg ccgttatcaa gtgctctgac cccatttttt acgtattacg atgtcacccc
    16441 ctacgggatt ttacaataat taatgagtca gcgattataa aatgtcatcc ctatgggatt
    16501 ttaagaaaaa aatacgcata cagggaatta tggcgtcttt gaatgcgggc acaggataga
    16561 taataggatc acatatattt tactcctcgg taaaccatga gatagtggca caaagctccc
    16621 gctatgataa aaaaatgaaa aa
//
LOCUS       DAOSOX010000034        15368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3870_length_15368_cov_9.930647, whole genome shotgun sequence.
ACCESSION   DAOSOX010000034 DAOSOX010000000
VERSION     DAOSOX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_15368_cov_9.930647"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLX22_04130"
     CDS             <1..945
                     /locus_tag="PLX22_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT19119.1"
                     /translation="DRVVFITTEQVVELPPNPFFDDPFFRQFFGIPQQKSRKQKRSGL
                     GSGFVLSSDGYICTNYHVIANVDSVKVKVKNKEYEATIVGYDQRTDLALLKINPGDKL
                     EPVFLGDSDQVKVGDWAIAIGNPFGLDRTFTVGVISATSRKDVDMMGGSQSHIQTDAA
                     INPGNSGGPLVNINGEVIGINRMIYSQSGGYMGIGFAIPINTAKSILEQLKKYKKVKT
                     GYIGVQIVPLTEEYANELGLKNTEGALVGGIMENSPAEKAGIQPGDVIISVDDKNIKS
                     YEDLIEIVNKTPIGKTLKVGVWRNKQKIYLFVTVKERP"
     gene            966..1175
                     /locus_tag="PLX22_04135"
     CDS             966..1175
                     /locus_tag="PLX22_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19120.1"
                     /translation="MNKYTHYILIIAGIVAVGYITFRYIIPLLFKLISFIVGFLAYGA
                     AVVLAVAIVLIIIGYIVRMYKTHSQ"
     gene            1252..3654
                     /locus_tag="PLX22_04140"
     CDS             1252..3654
                     /locus_tag="PLX22_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT19121.1"
                     /translation="MKERLKACLNSFKQFIIKYMHDTRTWFYGIIVVSLIGGLLFGYI
                     VAQVQNCLGIDNLKRFQPSVPTKLYDVNGELIAELFQEKRDLVSFEELPKTLIRAFVA
                     TEDQDFYTHIGISPMAMTRAMVKNIISLKIAQGGSTITQQLAKRLFTSGERTFTRKAL
                     ELVLTLQIEKKFSKDEILEMYFNQIYLGHGCYGIASAAELFFNKEVRFLSLAEGSVLA
                     ALPSAPGRYSPFMNTRNAYIKNWDILNRMVDCGYLTNEKAKEVYTSFWPIFVDTIKTE
                     FPTKTAYSKIVDNAPFFTDYVRQILISRYGKDVVYNEGLQVYTTLNLKRQQAGEKYLV
                     EGLTRQNEISARANQYLSGAVDRGLFSVYQQLGLLFNLPRIIVKSDTETIFKKYMVDN
                     LLNEMELLALLCNVDAMHNDIEKFMIVTSGISSSLKVEGALIAIEPKTGYITTMVGGS
                     EFAVSNQYNRSTQARRQPGSAFKPFVYGAGIESKLINTAMALPDAPIVDIDAQGSTWS
                     PNNYEGEFMGMVHLRRALAASINVISVRIYDIIGADTIIDFASRVLKVSPSRFHPGPS
                     LALGSSELTPLEMATGYAIYANRGRDVIPFSIRYITDRDGNEIENIEEDVGNIIAAKE
                     ADGSIQIIPENVNYIMTSLMQTVAEGGTPTEALRLIAKFDKKCAGKTGTTQNWTDAWF
                     CGFTPDIATVVWVGYDKPFMSLGRHQAGAAVAAPIWGFYMKEVYNGMPDPQWPAQPKD
                     VFYGATCKYSGCAPGPNCHELISDLMIPGGTPRCICNGIHYNMKSVFEQYMEKEGLKN
                     ED"
     gene            3815..4294
                     /locus_tag="PLX22_04145"
     CDS             3815..4294
                     /locus_tag="PLX22_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT19122.1"
                     /translation="MFKIGDKIVYPLHGVGIINAIEKKVVLNKRNEFYLITIINSGMK
                     VMIPTAKAESIGLRKVIPKKDVTKVLNLLRQTDIETEDDWKIRYQNNVDKIKSGSIFA
                     VAEVARDLYKRGKDKELSIMERKLYENAYQLMIHEIALAKDIDVDEAGNIVSDALSS"
     gene            4420..5421
                     /locus_tag="PLX22_04150"
     CDS             4420..5421
                     /locus_tag="PLX22_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOT19123.1"
                     /translation="MIAIIRFLFIITSAVVTGLYFFQISLQSAIIAAVIASAIALILI
                     IAIEYISHTISTRMVIAALVGIFIGLIFAHLLVIAFKSLPLTMQPNTVNIISAIIYHI
                     IGFGSVLFFIINCEDIAIFDAIVPVKRDEARDGASYKILDTSVIIDGRISDICDTGFI
                     EGILVIPNFVLNELQMIADSSDSIKRNRGRRGLDILNKMQKDQRILVKISDVDFPEVS
                     EVDAKLVKLAKLMTAKIITNDFNLNKVAEFHGVKVLNINQLSNALRPIVLPGEEMSVS
                     LIKEGKDPNQAIGYLDDGTMVVVENGRSRLNSTVDVIVTSVLQTTAGRMIFARIKED"
     gene            5399..5986
                     /locus_tag="PLX22_04155"
     CDS             5399..5986
                     /locus_tag="PLX22_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOT19124.1"
                     /translation="MQESKKTNYDVVVLCGSPHKEGNTVAILQLLFKELSNITKAIHF
                     AYELHVHPCIDCGFCHNEFGCIFHDDMDMLYATIQQSTLMIVASPLYFSHLPSPLKAI
                     IDRCQVFWEYYKNNPSQKKQHAMAILIGGGNYYDMFMPGKITLRHYFNSLQYHYDEDN
                     FLLLPNINSKNDIENADIMKKVEQTIHYIHTVLQR"
     gene            6118..>7215
                     /locus_tag="PLX22_04160"
     CDS             6118..>7215
                     /locus_tag="PLX22_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19125.1"
                     /translation="MKLYITLTFRCDDKNFLLFIQKVIQTDCTIQSINYLSSEQNYKI
                     YKAELVYTDRRLFKEKILQWITKECTLIECSDIMNQGIDGGLLKVSSKIPFETPDEFE
                     GKVLGYFELIKDNTITKDTYRYTGISKNIGMIILNEEKNELQPDEIYNYLLIERDSLI
                     ISHCNNYNPQPLLLKYKSKEDIIKTLYSVSGNYALMRVAIMNNIEDISLFHQMSEVTP
                     PIINREFDEIPLYITVKIMNLLKLHNIKLSDSVVGFIGVTTSLLRCTRLLNSIGCSKV
                     LGFDPNEKNLYYFEKESGIATSTDNVFENADILLFVSDICENIDYYKLQKGQLVLSNP
                     AIHFDMLISEERGVKANEYLNIENSYLLLPGI"
     assembly_gap    7216..7315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7316..7806)
                     /locus_tag="PLX22_04165"
     CDS             complement(<7316..7806)
                     /locus_tag="PLX22_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19126.1"
                     /translation="MQNATKIIIKYELISNLTHLLIMDNGGNKYYLLEHEHITEMLNN
                     VTHKLYDDLIITNNAYQVVYSMQNKNYYGRRIFDISPQLQQCLMKCNYNDISICEPSE
                     YPVMSGNFNFCVVYPITSNERNKGFLIGFINKIKLYDVLPEDAIIVDKYGMVIIKPEK
                     SIL"
     gene            8172..8789
                     /locus_tag="PLX22_04170"
     CDS             8172..8789
                     /locus_tag="PLX22_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOT19127.1"
                     /translation="MKKLQFISLILLISLFSCTSIDVAYTNKQIIQKRMKRVAVLPFE
                     ITGADWGNEFADAISHQFFKSGKVATIEREAIEKILQEQQLQASGLIDEKQAIKLGKL
                     FGADVIIIGRGTALQAYRKNKYEPNCIDTFTLKAINTENGEILLTVRKEPGIAWDWRY
                     RAQYCCGLTLIWDTNDILVESSQYDDIAKQLVKKILLIMENDKNK"
     gene            8786..9565
                     /locus_tag="PLX22_04175"
     CDS             8786..9565
                     /locus_tag="PLX22_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19128.1"
                     /translation="MKYLLITFFYSLFFLFIAVPFSNLFSSEFDELDKPPEGMHKDQL
                     LILGAVSIGMPYGNLITAEEKFTKKSSYQLTPETSKLIYLSHLAFGITISGEYMPADY
                     IGIRSKLRRSIIIQNTNFGSDYKSWKKTLYSDYSIYLGPSFHMTSRKPWDIALSPLIG
                     IAFSQYNPTPIAKELLDGYDGPPSEQFTSLTYGVDLLLSIFFSGGLVIQLGGEWVRNN
                     ITFSSTPNAENPQTHQIFYNKKSAVIDSILFHVSAGYAFYN"
     gene            complement(9735..10460)
                     /locus_tag="PLX22_04180"
     CDS             complement(9735..10460)
                     /locus_tag="PLX22_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19129.1"
                     /translation="MRLLIVIILTLSSIAPAYAQYDKAVKQFEQGNYDAALAEIASNL
                     DVSQDFEPNSLNYNLRYLAGHIHAKKGNYESALAHLKRCAEIKKDTIDPIIDIAFIYI
                     DKKSYTDAITWARKAIQIKQSAIAYYILGLSFSGIQSYWQAKEYLEKAIAIDPEMYYA
                     YNELGNVLMHLGKLSQAQTAYSAAYALYPSSAYICNNLALCYAKSGDIKKAKEMIGKA
                     HTFDASNTIITANYNRIINVANK"
     gene            complement(10535..13249)
                     /gene="ppdK"
                     /locus_tag="PLX22_04185"
     CDS             complement(10535..13249)
                     /gene="ppdK"
                     /locus_tag="PLX22_04185"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT19130.1"
                     /translation="MTKRVYLFGGNVTEGTADMKNLLGGKGANLAEMARIGIPVPAGF
                     TITTEVCNEYYKNNQKLTESLKEEVTEALRQVEGIMGAKFGDHSNPLLVSVRSGARAS
                     MPGMMDTILNLGINEDVIKGLVKKTGNERFAWDSYRRFIQMYGDVVLGLKPESKDEHD
                     PFEVIMDELKEKRNVKNDLDLTVEDLKELVASFKKLIQEKLKVTFPDDPYEQLWGAIG
                     AVFRSWNNERARLYRKMNNIDDSWGTAVNVQAMVFGNMGNDCATGVAFTRNPATGAKE
                     FFGEYLINAQGEDVVAGIRTPLQITLEGSRRWARENNISEDERKNKYPSLEEYMPEAY
                     NELVNVYKTLEKHYRDMQDIEFTIQNKKLWMLQTRNGKRTARAAINIAVDMVEEGLIT
                     KDEAVLRVDPQSLDQLLHPTFDPKAQRKVIAKGLPASPGAATGKIVFNAEDAHDWKGR
                     GEKVILVRIETSPEDLKGMNAAEGILTARGGMTSHAAVVARGMGKCCVSGCGMLDIDY
                     TKRQIKVDTTILKEGDYISIDGSTGEVMLGKVATMEPEVTGKFGTIMQWADEIRKLKV
                     RTNADTPHDARVARNFGAEGIGLCRTEHMFFEGDRIKAVREMILAPTYEGRKKALDKL
                     LPIQREDFYGIFKAMEGLGVTIRLLDPPLHEFLPQDIETQKEMAKELGVTVDDVKAKI
                     EELHEFNPMLGHRGCRLGITFPEITEMQSRAIFEAACQLVKEGIDVKPEVMVPLVGHV
                     NELIMQKEIIIKTAEVVMKEMGVTVKYMVGTMIEVPRAAITADEIAKVAEFFSFGTND
                     LTQMTFGFSRDDAGKFLKEYVDKKILSNDPFRVIDRDGVGQLIKMACEKGRSVNPNLK
                     LGICGEHGGDPSSVEFCHVVGLDYVSCSPFRVPIARLAAAHAAIRFPK"
     gene            complement(13438..14376)
                     /locus_tag="PLX22_04190"
     CDS             complement(13438..14376)
                     /locus_tag="PLX22_04190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT19131.1"
                     /translation="MNKNWAVSIIIVNGLIFLVQLLLRNVFVPYDIAGNVVSIDIVTY
                     YLGLIPQLVVEKLYIWQIISYMFLHSTSSFAHILFNMYAVFIFGMPVERLWGSKKFII
                     YFLICGIGAGVAIFTIALITGGMGYYIPTIGASGAVFGLLLAFGVLFPNVEILLFFII
                     PIRARTLVILYGGLELFLELSGGADNISHIGHLGGLATGILYFIVTRKHAIRYKTKGF
                     VAAQMKKETIPDKPDVNYKREAELQRAIVQKIREHGVESLNDDEVQYIRYKEIMIDIE
                     EEEVPCDLQIFLDDNCNDCENRDACFIKIVKSFKKE"
     gene            complement(14412..14732)
                     /locus_tag="PLX22_04195"
     CDS             complement(14412..14732)
                     /locus_tag="PLX22_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOT19132.1"
                     /translation="MGKKECIRCGACCFVDMVAYATPEDFLRWEKEKRYDILHIIENE
                     NSIWAGDHLISTRDGHLIGRCPFLIMENNGLYSCMIYDTRPAVCKDFEPGVSIMCPLF
                     ALKE"
     gene            complement(14746..>15368)
                     /locus_tag="PLX22_04200"
     CDS             complement(14746..>15368)
                     /locus_tag="PLX22_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19133.1"
                     /translation="FLLNFEESTMAGTGVRENGKQRRLIMEKSQVLFWEKGYAETSMK
                     DIANECGFRPANIYNFFASKEEILFFILKEEMEEIVNPIRFLEYEENDPLQQLRFLIE
                     IHVKITLGEKRASKLLFDTELRNLSAERRKVILDLRDDYNRIGYSILKRGINKGLFRA
                     IDEKIALNLIASMIVRTRLWFSPEDWMTIEDLVEFIFQFALKGLSK"
BASE COUNT         5037 a         2702 c         2447 g         5082 t
ORIGIN      
        1 gacagggtag tttttattac aaccgaacag gtggttgagt tgcctccaaa cccttttttt
       61 gatgatccct ttttccgcca attttttggg attccccaac aaaaatcacg taaacaaaaa
      121 cgttccggat tggggagtgg ttttgttcta tcaagcgatg gttatatctg tactaactac
      181 catgtcattg cgaatgttga ttcagtaaag gtaaaggtta aaaataaaga gtacgaagct
      241 acaattgttg gatatgacca gagaacagat ttggcattac tgaaaataaa tcctggcgat
      301 aagcttgaac ctgttttttt aggtgactct gatcaagtaa aggttggaga ttgggcaatt
      361 gctattggaa atccctttgg gttggaccgt acatttactg ttggagttat tagtgctaca
      421 agccgaaagg atgtagatat gatgggtggt tcacagtcgc atatacagac tgatgcagca
      481 attaaccccg gaaattctgg tgggcctctg gttaatatta atggggaggt tataggcatt
      541 aaccgcatga tttattccca gagtggtggc tatatgggga ttggatttgc aatccctatt
      601 aatacggcta agtctatttt agagcagtta aagaagtata aaaaagttaa aaccggctat
      661 attggcgtac agatagttcc attgacagag gaatatgcaa acgaattggg gcttaaaaat
      721 actgaaggtg ctcttgttgg tggtattatg gaaaacagcc ctgctgaaaa agctggtata
      781 caaccaggtg acgtcattat atcagttgat gataaaaata tcaaatcata tgaagatctt
      841 atagaaattg ttaacaagac acctattggc aaaaccttaa aagttggcgt atggcgtaat
      901 aaacaaaaaa tctacttatt tgttacggta aaagaaaggc cttagatatt aaaagcatgc
      961 acagtatgaa taagtacaca cattatatat taataatagc gggaatagtt gctgtgggat
     1021 atataacctt tcgctatatt atcccgctat tatttaaatt aataagcttt atagtaggct
     1081 ttttggctta tggagcagca gttgttttag ctgtagcgat agtattaata attattggct
     1141 atattgttcg gatgtacaaa actcattctc agtaaataac ttgataccac tatccatttg
     1201 aatgctaaac taagctatag taatataata aataattgag gtcagaacaa cgtgaaagag
     1261 cgtttgaaag cctgcttaaa cagttttaaa cagtttataa ttaaatacat gcatgacaca
     1321 cgcacatggt tttacgggat tatagtagta tcactcatcg gcggtttgct ttttgggtat
     1381 attgttgctc aggtacaaaa ttgtcttggt atagataatc taaagcgttt ccagccaagt
     1441 gttcctacta aactgtatga tgttaacggt gaacttattg ccgaactttt tcaagaaaaa
     1501 cgtgatttag tatcatttga agaacttcca aaaacattga taagggcgtt tgttgcaact
     1561 gaggatcagg acttctatac gcatatagga ataagtccta tggcaatgac acgggcaatg
     1621 gttaaaaata ttatttctct taaaattgca cagggcggct caaccattac ccagcaattg
     1681 gcaaaacggc tttttacgtc aggcgaaaga acatttaccc gtaaagcatt agaattagtt
     1741 ttaacattgc aaattgaaaa gaaattctcc aaagatgaaa ttcttgaaat gtactttaac
     1801 caaatttatt taggacacgg atgttatggg attgcatctg ctgctgagct tttcttcaat
     1861 aaagaggtta gatttcttag ccttgccgaa ggaagcgttt tagctgcatt accttcagct
     1921 cctggtcggt actccccatt tatgaatacc cgtaatgctt atattaaaaa ttgggatatt
     1981 ttaaacagaa tggttgactg cggctattta actaatgaaa aagctaaaga agtatatacc
     2041 tctttctggc ccatttttgt tgataccata aaaactgaat ttcctactaa aaccgcatat
     2101 tcaaaaatcg tggataatgc cccatttttt acagattatg tacgtcagat acttatttcc
     2161 cgatatggca aggatgtagt gtataatgaa ggattgcaag tatataccac attaaacttg
     2221 aaacgacaac aagcaggtga aaaatatctt gtggaaggtt taaccaggca aaatgagata
     2281 tctgctcgtg ccaatcaata tctttcagga gctgttgata gaggattatt ctctgtatac
     2341 cagcaattag gacttctttt taatttaccc agaattatcg tcaaaagtga tacagaaaca
     2401 atattcaaaa aatatatggt tgataatctg ttgaatgaaa tggagctttt agcgttatta
     2461 tgcaatgttg atgcaatgca taatgatatt gaaaaattta tgatagtaac cagtgggatt
     2521 tcttcttcat taaaagttga aggggctctt atagctattg aacctaaaac tggatatata
     2581 acgacaatgg ttggtggatc agaatttgct gtgagcaatc aatataacag atcaactcag
     2641 gctcgccgtc agcctggatc agcatttaaa ccatttgttt atggtgcagg tattgagtca
     2701 aaattaatta atacagccat ggctcttcca gatgcaccta ttgttgatat tgatgctcag
     2761 ggaagcacct ggagcccgaa taactatgaa ggcgaattta tgggaatggt tcatttaagg
     2821 agggctcttg ccgcatcaat caatgtaatt tcagtacgga tatatgatat cataggtgca
     2881 gatactatca tagattttgc atcacgcgta ttaaaggttt ctccttcacg cttccatccc
     2941 gggccatcat tagctttagg aagttcagaa ttaacaccat tagaaatggc aacaggatat
     3001 gcgatttatg ctaacagagg gagggatgtc attccatttt ccattcgtta tattaccgat
     3061 agagatggca acgaaataga aaatattgaa gaagatgttg gcaatatcat tgcagcaaaa
     3121 gaagcagacg gttcaattca gattattccc gaaaatgtaa attatataat gacatcatta
     3181 atgcagactg ttgccgaagg aggtacacct actgaggctt tgcgcttaat tgcaaaattt
     3241 gacaaaaaat gtgcaggcaa aacaggcact actcaaaact ggacagatgc atggttttgt
     3301 ggttttactc ctgatattgc aacagttgtt tgggttggat atgataaacc ttttatgtca
     3361 ttgggacgac atcaggctgg tgcagcagta gcagcgccca tatggggatt ttacatgaag
     3421 gaagtttaca atggaatgcc ggatcctcaa tggccagctc aaccaaaaga tgttttttat
     3481 ggtgctacct gtaaatattc aggctgtgca cctggtccaa attgtcatga attaatcagt
     3541 gatttaatga tacctggcgg aactcctcgt tgcatttgta atggaataca ctataatatg
     3601 aaatctgttt ttgaacaata tatggaaaaa gaaggtttaa aaaatgagga ttaatagcta
     3661 ttgtcaaatt tcacaatcaa ataacaaact ctattactag ccgataatac tttcttaaaa
     3721 aaaattttct acttgatttt attaaaatta tgtgctaaaa ttattaattt atacgttata
     3781 ataaatgtgg gttaatttat tttgagggta tcctatgttt aaaataggtg ataagatagt
     3841 gtatccttta catggggtag gtatcataaa cgctatcgaa aaaaaggtag tattaaacaa
     3901 aaggaatgaa ttttatctta taacaatcat caacagtggc atgaaggtta tgattcctac
     3961 cgcaaaggca gagtccatag ggttgaggaa ggttattcca aaaaaggatg taacaaaagt
     4021 attaaatctg ttacgccaaa cagacattga gaccgaggat gactggaaaa ttcgttatca
     4081 aaacaatgtt gataagatta aaagtggatc aatatttgca gtggctgagg ttgccaggga
     4141 tctatataaa cgtggaaaag ataaagagct ttctataatg gagcgaaagc tttacgagaa
     4201 tgcttatcag ctcatgatac atgagatagc tttagcaaag gacattgatg ttgatgaagc
     4261 aggcaacatt gtatcagatg cactttcctc ataattgctc actacagttt ataatttttg
     4321 tatcagtcac tttatttata aaatatttaa ctttatggat ccataatgtt ttttttatca
     4381 aaattttatt ttaatatata taaaggagtc agtatatcca tgatagcaat tatacgtttt
     4441 ttatttatta ttacttcagc tgttgttacc ggtttatatt ttttccaaat ttcattgcaa
     4501 tcagccataa tagctgctgt gattgcatct gctatagcac ttatactcat tattgcaata
     4561 gaatatattt cacatactat ctcaacaagg atggttattg cagcgctggt tggcattttt
     4621 ataggactca tctttgcgca tcttttagtg atagcattta aatcattacc ccttaccatg
     4681 caaccaaaca cggtaaacat tataagtgcc attatttacc acattatagg atttggatcc
     4741 gtattatttt ttataataaa ttgtgaggat attgcaattt ttgatgcgat agttcctgta
     4801 aaacgtgacg aagcaaggga tggcgcatca tataaaattc ttgataccag cgtcattata
     4861 gatggtcgta tttcagatat ctgtgatact ggatttattg aaggtattct tgtgattccc
     4921 aattttgtat tgaatgaatt gcaaatgata gctgattcat ctgattctat taagcgtaat
     4981 agaggaagac gaggtttaga catactcaat aaaatgcaga aggatcagag aattttagta
     5041 aaaatttctg atgttgattt tcccgaagtt tcagaagttg atgcaaaatt ggtcaaatta
     5101 gccaagctta tgactgcaaa aattattacc aatgatttca atctaaacaa agttgctgag
     5161 ttccatggcg ttaaagtttt aaatattaac cagctttcaa acgccctcag gccaatagtg
     5221 ctaccgggtg aagaaatgtc tgtttcatta ataaaagaag ggaaagaccc caatcaagca
     5281 attggatatc ttgatgacgg gactatggta gtagtggaaa atgggagatc acggttaaat
     5341 tcaacggtcg atgtgatagt tacttcagta ctccaaacaa ccgcagggag aatgatcttt
     5401 gcaagaatca aagaagacta attatgatgt ggttgttctt tgcggaagtc ctcataaaga
     5461 aggaaatact gttgcaattt tgcaattact ttttaaggaa ctatcgaaca taacaaaagc
     5521 aatacatttt gcctatgaac tacatgtaca tccatgtata gactgtggat tttgccataa
     5581 tgaatttggc tgtattttcc acgacgatat ggatatgctt tacgctacca tacaacaaag
     5641 tacattaatg attgttgcat caccattgta tttttcgcat cttccttcac ctctaaaagc
     5701 gattatcgac cgctgccagg ttttctggga atactataag aataatccat cacaaaaaaa
     5761 acagcatgca atggctatat taattggtgg tgggaattat tatgatatgt ttatgccggg
     5821 gaaaattaca ttacgacatt atttcaattc attgcagtat cactatgatg aggacaactt
     5881 cttattatta ccaaacataa atagcaaaaa tgatatagaa aatgccgata ttatgaaaaa
     5941 ggtagaacaa acaatccatt atatacatac agtattacaa agatagtagt agttatgcat
     6001 tcccttttat aaggacactt tgatgggttt agctttactg aaagtagata aatgtttatt
     6061 attagcaatt ttttcccgat aaaacaatga taatgattgc ttttttttgg aggaaatatg
     6121 aaattatata ttactctaac cttccgatgt gatgataaaa attttttatt atttattcaa
     6181 aaagtcatcc aaacagattg taccattcaa agcattaatt atttatcatc tgaacaaaac
     6241 tataaaatat ataaagcaga acttgtttat actgatagaa gattatttaa agaaaaaata
     6301 ttacaatgga taacgaaaga atgcacactc atagaatgca gtgacattat gaatcaaggc
     6361 atagatggtg gtctgcttaa agtctcaagc aagataccat ttgaaactcc agatgaattt
     6421 gagggcaaag ttctgggata ttttgagttg attaaagata ataccataac aaaagatacc
     6481 tatcgataca caggtatatc aaaaaatatt gggatgatta tcctaaatga agaaaaaaat
     6541 gaattgcaac cagatgaaat atataattat ctcctgatcg aacgtgattc actcattatt
     6601 agccattgca ataattataa tcctcaaccc cttttgttga aatataaatc aaaagaagat
     6661 attataaaaa cactttattc tgtttcaggc aattatgcct taatgcgtgt tgccattatg
     6721 aataacatag aagatatatc attattccat cagatgagtg aggtaacacc ccctattatt
     6781 aatagagaat ttgatgagat accactttat attactgtaa aaataatgaa tctattgaag
     6841 ttgcataaca tcaaattatc agattccgtt gttggattca ttggtgtaac aacatcttta
     6901 cttcgatgta ctcgattgtt aaacagtatt gggtgtagca aagtattagg ttttgatcct
     6961 aatgaaaaaa acctttacta ttttgaaaag gaaagcggta tagcaaccag cactgataat
     7021 gtctttgaaa atgcagatat acttttgttt gtatcagata tttgtgaaaa tatcgattat
     7081 tataagcttc agaaaggaca attagtattg tctaatccag ccattcattt tgatatgtta
     7141 atatctgaag aaagaggtgt taaagcaaac gaatatctta atatagaaaa ctcatatctt
     7201 cttttgcctg gtattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttaa
     7321 aatactcttc tcaggtttta ttataaccat tccgtattta tccacaatta ttgcatcttc
     7381 tggtaaaaca tcataaagct tgattttatt aataaatccg ataagaaaac ctttatttct
     7441 ttcgttagac gtaataggat aaacaacaca aaagttaaaa tttcctgaca tcacaggata
     7501 ttcagatggt tcacagatag atatatcatt gtaattgcat ttcattaaac attgctgtaa
     7561 ttgaggcgat atatcaaata ttctgcgtcc ataataattt ttattttgca tcgagtaaac
     7621 aacctgatag gcattattag taattataag atcatcataa agcttatggg taacattatt
     7681 gagcatttcg gtaatatgtt catgttccag taagtaatat ttattcccac cattatccat
     7741 tataagcaga tgagtcaaat tggaaataag ttcatatttg ataataattt tagttgcatt
     7801 ttgcatatcg cacacataat gtgaaaaaaa agatacaact ggattgttat aattgttagt
     7861 tatttgccta ttagtacaag acgaaattaa agttataaga agtataatgg ttgcaactct
     7921 aatatattgt aaaatatggc attgtattct aatactcaaa tctatcataa tcctttcatt
     7981 atttgtattt ttttattata ctatacatat ttataatcgg ttttatcact gaatcttaca
     8041 ataaaaagga atcaagtaaa tagcatatta ataattaata atctaataaa ataatatttg
     8101 ccaaaattta tattcaaatg gtataattaa ataatttata aacttattta aataattcaa
     8161 aaggttctat tatgaaaaaa ttacaattta ttagcttaat acttttaatt tctttatttt
     8221 catgtaccag catagatgta gcatatacca acaaacagat cattcaaaaa agaatgaagc
     8281 gtgttgcagt tttacccttt gagataaccg gagctgattg gggcaatgaa tttgccgatg
     8341 ctatctccca tcaatttttt aaaagtggca aggtagcaac aattgaaaga gaagcaattg
     8401 aaaaaatatt gcaggagcag caattgcagg cttccggatt aattgacgaa aaacaggcta
     8461 taaaattagg aaaattattt ggtgctgatg taataataat cggtcgaggt acagcactcc
     8521 aggcatatcg taaaaataaa tatgaaccta actgtattga tacatttact ctcaaagcta
     8581 ttaatactga aaatggcgag attcttctta cagtgcgtaa ggaaccaggc atagcatggg
     8641 attggcgcta tagagcacaa tactgttgtg gacttacatt aatctgggac accaatgata
     8701 tattagttga aagttcacaa tatgatgata tagcaaagca actggttaaa aaaatactac
     8761 tcattatgga aaatgataaa aataaatgaa atatttatta ataacttttt tttattcatt
     8821 gtttttttta tttatagctg taccattttc caatttattt tcatccgaat ttgatgaatt
     8881 ggataaaccc cccgaaggga tgcataagga tcagcttctt atacttggtg ctgtttcaat
     8941 tggcatgcct tatggcaatc ttataacagc agaagaaaaa ttcacaaaaa aatcatcgta
     9001 tcaacttaca cccgaaacct caaagcttat ctatctttcc catctggcat ttggaataac
     9061 aatttctggt gaatatatgc ctgcagatta tataggtatc cgttctaaat taagaaggtc
     9121 tataataata caaaacacaa attttggttc tgactataaa tcatggaaaa aaacattata
     9181 tagtgattac tctatctatt taggtccctc ttttcatatg acatctcgga aaccatggga
     9241 tatagctctg tctcctttaa ttggtatagc attttcgcaa tataatccca ctcctatagc
     9301 aaaagaacta ctggatggtt atgacggccc accttcagaa caatttacca gcttaaccta
     9361 tggtgttgat ttactgttaa gcatattttt tagtggtggc cttgttatac aacttggggg
     9421 agaatgggtt cgtaataata taacattttc ttctacccct aatgcagaaa atccacaaac
     9481 tcatcaaatt ttttataaca aaaaaagtgc tgttattgat tctatacttt ttcatgtttc
     9541 tgctggatat gctttctata actgataatt gctctttctt tttgttatat aataggatgg
     9601 atacaaaaag caggatataa ataagtatag ttatttatta attcactatc aggaatacga
     9661 tatagaaaat acttaagtac ctaacctgtt attaattaca tttgataaac accgtaatac
     9721 acttgcaacg taaattattt gttcgctacg ttaataattc tattgtaatt tgccgttatg
     9781 attgtatttg atgcatcgaa tgtatgtgct ttcccaatca tttctttagc ttttttgata
     9841 tctcctgatt tagcataaca cagtgctaaa ttattacata tatatgctga agagggataa
     9901 agtgcatacg cagcagaata agcagtttga gcctgactta atttacccag atgcattaat
     9961 acattcccta attcattata tgcataatac atctcaggat caattgcaat agctttttct
    10021 aaatactctt ttgcttgcca atacgattga atccctgaaa acgataatcc taatatataa
    10081 taagctatag ctgattgttt tatttgaata gctttccttg cccatgtaat agcatcagta
    10141 taactttttt tatcaatata tataaaagca atatcaataa ttggatcgat ggtatctttt
    10201 ttaatttcag cacaccgttt taaatgtgct aaagcggatt catagttacc tttttttgca
    10261 tgaatatgtc cagctaaata acgcaaatta taattgaggg aatttggttc aaaatcttgt
    10321 gatacatcaa gattagatgc tatctcagct aacgccgcat cataattacc ctgctcaaat
    10381 tgctttacag ccttatcata ttgagcatat gccggtgcaa tactactaag tgttaaaatt
    10441 atcacaatta ataatcgcat agttatctga acctcctgca aactaataga acgtctatct
    10501 attataattc atagatagac gttctctaca aacatcattt tggaaaacgg atagcagcat
    10561 gagctgcagc taaccgtgca atgggaactc taaaaggtga acagcttaca taatccagac
    10621 caaccacatg acaaaactca acggaacttg gatcaccacc atgctccccg caaataccta
    10681 attttaggtt tggattcact gaacgacctt tttcacatgc catttttatt aactgtccta
    10741 caccatctcg atcaataacc ctgaatggat cattagataa tatcttctta tcaacatatt
    10801 cttttaaaaa ttttccagca tcatcacggc taaatccaaa ggtcatctgg gttaagtcat
    10861 tagttccaaa cgaaaaaaat tcagcaactt ttgctatctc atcggctgtt atagcagctc
    10921 gtggaacttc tatcattgta ccaaccatgt atttaactgt tactcccatt tctttcataa
    10981 ctacttcagc cgtttttatt attatttcct tttgcatgat aagttcatta acatgtccaa
    11041 ctaaaggaac cataacttcc ggtttaacat caataccttc ttttaccagc tggcacgctg
    11101 cttcaaatat tgcacgtgat tgcatttcag tgatttcagg gaatgttatc cccaaacggc
    11161 accctctatg tcctaacatt ggattaaatt catgaagttc ttcaattttt gcttttacat
    11221 catcaacagt aacacctaac tcttttgcca tctctttctg tgtttctata tcctgaggta
    11281 aaaactcatg tagtggtgga tcaagtaaac gtattgttac gccaagacct tccatagctt
    11341 taaatatacc ataaaaatct tcacgttgta ttggcaataa tttatcaaga gctttttttc
    11401 tgccttcata ggtaggggcc aatatcattt cacgaacagc ttttattcta tcaccttcaa
    11461 agaacatatg ctccgtacga caaagcccaa ttccttcagc cccaaaattt cgtgccaccc
    11521 tggcatcatg cggagtatct gcatttgttc ttacctttaa tttccttatt tcatccgccc
    11581 actgcataat agtaccaaat tttcctgtta cttcaggttc catagtagca acttttccaa
    11641 gcatcacttc ccctgttgac ccatcaattg agatataatc tccttcttta agaattgtag
    11701 tatcaacttt tatctgccgt ttagtataat ctatatccag cattccacac cctgatacac
    11761 agcatttacc catacctcta gctacaactg cagcatgaga agtcatgcca cctcgtgctg
    11821 ttaaaatccc ttctgcagca ttcattccct taagatcctc cggagatgtt tctattcgta
    11881 ccaaaataac cttttcacca cgccccttcc aatcatgtgc atcttcagca ttgaatacaa
    11941 tcttccctgt tgccgcccct ggtgacgcag gtagaccttt agcaatcact ttacgctgag
    12001 cttttggatc aaatgttgga tgtaatagct gatctaatga ttgaggatca actcttagta
    12061 ctgcttcgtc ttttgtaatt aaaccttctt caaccatatc aactgcaata ttgatagccg
    12121 cgcgagctgt tcttttgcca ttacgtgttt gcagcatcca caatttttta ttttgtatgg
    12181 taaattctat atcttgcata tcacgataat gtttttcgag ggtcttatat acattaacaa
    12241 gctcattata tgcttcaggc atgtactcct caagtgaagg atatttattc ttacgttcat
    12301 cttcagagat gttattttct cgtgcccaac gtcgcgaccc ttccagtgtt atctgtaacg
    12361 gtgttctgat accagcaacc acatcctcac cttgagcgtt aattaaatat tcgccaaaaa
    12421 attcttttgc tcctgttgct ggattcctcg taaatgcaac acctgttgca caatcatttc
    12481 ccatattacc aaacaccatt gcctgcacat tgacagcagt accccagcta tcatcaatat
    12541 tattcatctt ccgatagagt cgtgcacgtt cattgttcca tgatcggaat acagcgccta
    12601 tagctcccca gagttgttca taaggatcat cagggaaggt cactttaagt ttttcctgta
    12661 taagtttttt aaagcttgct acaagctctt ttaaatcttc aacagttaga tcaagatcat
    12721 tttttacatt tcttttttct tttaattcat ccattataac ttcaaatgga tcatgttcat
    12781 ctttactttc tggtttaaga cctaaaacaa catcaccata catttggata aatctgcggt
    12841 atgaatccca tgcaaaacgt tcatttcctg tctttttcac aagacctttt atgacatcct
    12901 cattaatacc cagatttaaa atagtatcca tcattccagg cattgaagca cgagcacctg
    12961 aacgaaccga aaccaataat ggattggaat gatcgccaaa cttagcaccc attatccctt
    13021 caacctgacg caatgcttct gtaacctctt cttttaaact ttccgtcaat ttttgattgt
    13081 ttttataata ttcattacat acttctgtag taattgtaaa gcctgccggc accggtattc
    13141 caattcttgc catttctgcc aggtttgcgc ccttaccacc caataagttt ttcatgtcag
    13201 cagtaccttc agtaacatta ccgccaaaca gatatacgcg ttttgtcatt gcaattctcc
    13261 cttctaataa aattttgtta ttatcattac aaaaatgata atcatatttt ttacattatc
    13321 cagcaagaag aatctttata taaatattaa ttttttaaaa cttgtacatt gttagtatac
    13381 tacaattttg ttgtaaagat taattttaca ttatcaaaat cattttattg gtgtattcta
    13441 ttctttctta aatgatttca caatttttat aaaacatgcg tctctgttct cacaatcgtt
    13501 acaattgtca tctaaaaata tttgtaaatc acagggtacc tcctcttcct ctatatcaat
    13561 cattatctct ttatagcgta tatattgaac ttcatcgtca tttaaacttt caactccatg
    13621 ctccctgatt ttttgtacta ttgccctttg tagttctgct tcccttttat aattaacatc
    13681 cggtttatcg ggaatagttt cttttttcat ctgtgctgca acaaatcctt ttgttttata
    13741 gcggatagca tgcttgcgag taacaataaa atacagaata cctgttgcaa gccctcctaa
    13801 atggccaata tgtgaaatat tgtctgcccc ccctgatagt tctaaaaata attccaatcc
    13861 tccataaagt atgaccaatg tccttgctct aatcggaata atgaaaaaga gtaatatctc
    13921 aacattaggg aataacacgc caaatgctaa aagtaatcca aaaacagcac ctgatgcccc
    13981 gattgtaggt atataatatc ccataccacc agttatgaga gctatcgtaa agatcgctac
    14041 gccagcccct ataccacata ttaaaaaata tattataaat tttttactac cccacaatcg
    14101 ttcaacaggc ataccaaaaa taaaaaccgc atacatatta aaaaggatat gagcaaaact
    14161 tgatgtgcta tgcaaaaaca tataagaaat aatttgccat atatataatt tttcaactac
    14221 caattgagga attagcccca gataatacgt tacaatatca attgacacaa catttccggc
    14281 aatatcatat ggaacaaaca cattacgtaa taacaattga actaaaaaaa ttaatccatt
    14341 tactataatt atggaaacag cccagttttt attcattttt ttatcctgct cagttattaa
    14401 ttggcaattt attattcttt taatgcaaac agtgggcaca ttatagaaac accaggttca
    14461 aaatccttgc atacagcagg tcttgtatca taaatcatgc agctatataa cccattgttt
    14521 tccattatca aaaaaggaca tcgtccaatt aaatgtccat cacgcgttga tattaaatga
    14581 tctcctgccc atatactgtt ttcattctct attatatgaa gtatatcata tcttttttct
    14641 ttttcccagc gtaaaaaatc ttctggtgtt gcataagcaa ccatatcaac aaaacaacat
    14701 gcaccacaac gtatacattc tttttttccc ataaaaaaat tataactact ttgaaagccc
    14761 tttaagagca aattgaaaaa taaattctac caaatcttca attgtcatcc aatcttcagg
    14821 ggaaaaccat aagcgtgttc tcactatcat agaagcaatc agatttaacg caatcttttc
    14881 atcgatggct ctaaacaacc ctttattaat tcctcttttt agtattgaat atcctattct
    14941 gttataatca tcgcgtaaat ctaatattac ttttcttctt tctgctgaaa gattcctgag
    15001 ttctgtatca aataataatt tggaagctct tttctcccct aaagtaattt ttacgtggat
    15061 ttctattaga aagcgtaatt gttgtaaagg atcattttct tcatattcca gaaaccgtat
    15121 aggattaaca atttcttcca tctcttcttt taaaataaaa aaaagtatct cttctttact
    15181 tgcaaaaaaa ttgtatatat ttgcaggtct gaaaccacat tcattagcaa tatctttcat
    15241 acttgtttcg gcatacccct tttcccagaa gagaacctga gacttttcca taattaatct
    15301 tctttgtttg ccattttctc taacacctgt accagccatt gtagactcct caaagtttaa
    15361 caaaaaaa
//
LOCUS       DAOSOX010000035        15296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3894_length_15296_cov_8.969359, whole genome shotgun sequence.
ACCESSION   DAOSOX010000035 DAOSOX010000000
VERSION     DAOSOX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_15296_cov_8.969359"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLX22_04205"
     CDS             complement(<1..441)
                     /locus_tag="PLX22_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT19134.1"
                     /translation="MESRNVLLIFNQNIMYKPIIYRIAKDFNVIFNVLEAKILPKQEG
                     RLILQLSGEQEDIDTCINYLINEEVIVDILADKIQRDEDRCVHCGACTAVCRTDALVI
                     NRETMMVEFYPERCVACGLCKLACPVKAMSGISIDVDSEELAIKV"
     gene            complement(453..1178)
                     /locus_tag="PLX22_04210"
     CDS             complement(453..1178)
                     /locus_tag="PLX22_04210"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOT19135.1"
                     /translation="MNNTLAYRALIQDTRFTTFEVCVETTSLFVCAHNNLGDRTLQLV
                     HWLRNDIKDYIAKHDNFLHALSPLEFDPSAPEVVRRMYSASAKASVGPMAAVAGTIAQ
                     MIGEELTAYSPEIIIENGGDIWAKVQEPAVIKVFAGRSSFSDDCGIVIQPHDTPLGIC
                     SSSGRFGHSFSFGKADCVTILASSASLADAVATATCNRVQTEEDCVHALEYACTISGV
                     TGALVIYWDTMAMKGCIELTQTS"
     gene            complement(1212..2402)
                     /locus_tag="PLX22_04215"
     CDS             complement(1212..2402)
                     /locus_tag="PLX22_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOT19136.1"
                     /translation="MSDIVIKKSYEAINNKIKKGEAVVVTADEMTSIVKSHGYKSAFK
                     KVDVVTTGTFGTMCSSGAFINFGHTRPKIKASKVWLNDVEAYAGLAAVDIYIGATQVQ
                     EDDPLNKVHPGRFKYGGGHVIEDLIAGKPVKLKAKSYGTDCYPLREYEATVTINDLRD
                     AFLYNPRNAYQNYNCAVNQSKKTIYTYMGILRPNMGNATYSSAGQLSPLLNDPYYWTI
                     GVGTKIFLAGSVGAVTWRGTQHSPNEPRSENGVVTSGAGTIAVTGDMKHMTTQFIKGA
                     SITGYGCSLMVGIGIPIPILNEDLAFFTGVSDDAIYCQVVDYGYDYPNAISRSIAQVS
                     YAQLKSGFVEIAGKRIPTAPLSSYPMAKKIADILKDWIVQRQFTVGEPQILLPTVPYQ
                     KHHE"
     gene            2701..3321
                     /locus_tag="PLX22_04220"
     CDS             2701..3321
                     /locus_tag="PLX22_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19137.1"
                     /translation="MPRLKPHARAEKEELRKTQITQSAYEVISAKGYNNFTLDDIAKN
                     AGLSKGGVLHYFKSKEDILLYLLEQIYIIIKKNINKRSSKYRTPERRLRAIVIAFIIT
                     AKRHPAFYTVLVDFWAQIPIHERVKYINTQIYKQLCDEVIKVIEDGIASGVFKQVHAD
                     NTAHAIVAMVMNVAIQWTFNHELYNIDHMAKTCMKMIMAYLEEKTI"
     gene            complement(3314..3967)
                     /locus_tag="PLX22_04225"
     CDS             complement(3314..3967)
                     /locus_tag="PLX22_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19138.1"
                     /translation="MAQATSQDIVHEILSAINRNQEIVFVSYSILDETEKNIQFALNK
                     ILERHNKSDLYTPVYSSLKELVANAIKANAKYILIEEGEIKDPNDIMEVVNKVRNILN
                     EESLLLYGIKAKQYRLSTRIYLKPQTTRLTIEVINNLPVPQKELERMRERIERSSYYD
                     SIADFYLENPDPTAEGMGLGLSMVVVLLKSVGIPYTNFTLYTDFKSKTVARIIIPLI"
     gene            4111..4974
                     /locus_tag="PLX22_04230"
     CDS             4111..4974
                     /locus_tag="PLX22_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOT19139.1"
                     /translation="MFNMLFPSVSSITVITLLSLVCGILLSLAKVLLKVEKDPRIEKI
                     VESLPGANCGACGFPGCVGYATHIIEKGASINLCPVGGPESAQKISQIMGIEAEIVAK
                     RIARVHCQGGINETKIRFMYDGPHSCTAAQLVMGGFKVCEYGCLGLGDCVRACLFDAI
                     HLDKRGLPLVDAEKCTGCGNCVEACPRNIISLLGENIDVYMMCRNKQKAPVMKLGCNV
                     GCTACRRCEKVCREEVFKDNPDVDTAIKVVDFLAVIDYELCINCGRCAEVCPQNVIDF
                     KKVMVIKRETN"
     gene            4961..5977
                     /gene="fliG"
                     /locus_tag="PLX22_04235"
     CDS             4961..5977
                     /gene="fliG"
                     /locus_tag="PLX22_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOT19140.1"
                     /translation="MKPIDQMSGAERAAALLVALGPEVASEIMKFLDDESIQKLASEI
                     AKIEVLSPQEKEDLIGQFLLELKKTRGITFGGQNVAKEILYAAFGQDKAQDILKRLTH
                     KDLEKGFGFLNDIDPELIVTFLHNEHPQTIAVTLAYLPAAKAAEVIKLLPTDIAKEVT
                     LRMAKMERTSPDAVLEISRVLRKKYDEYRYQGQSSSKAGGLNALIDIMSHLSGDQEKQ
                     LIDYFETTMPQIADEIRARIFTFENVITLTNTEMRILIDEINNDYMIARALKGAGDEI
                     RFKFFRNMSKNRATDIMNEMEAMGPVRLSEIQSARDNIIRVMRILHDNGVISLRKEKE
                     KYIE"
     gene            5970..6803
                     /gene="rsmA"
                     /locus_tag="PLX22_04240"
     CDS             5970..6803
                     /gene="rsmA"
                     /locus_tag="PLX22_04240"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT19141.1"
                     /translation="MNKKEILSWLQQYGLAPNKMYGQHFLFHEKYIEAIVTAISPCSE
                     DVILEIGPGLGVLTQRLSEHAKKVIAVEIDAGFVEVLQSRFANNSRVTIVHNDFLKTN
                     PYDECTVLTGNLPYNIASQIIFAAAHNFTAPHCFFLLQKEMAQRLIAKPKSAMYSAFT
                     VGIGLYFTVQTLFDIPPQAFYPEPKVYSTFVKLTRKNSVMLHTDEKAVFEQLVATAFW
                     ARRKMLKTALKRSPYTTYREAFISQAFGQLGFDEKVRGEELTLDEFVALAKYCAHYGN
                     K"
     gene            6825..9257
                     /locus_tag="PLX22_04245"
     CDS             6825..9257
                     /locus_tag="PLX22_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT19142.1"
                     /translation="MIPEETIYNLLTTVSKPARYAGGEWNAVVKPHARVKVGLCFPDL
                     YEVGMSNNGIQILYHAINGMQHAAAERVFAVEHDFENQLRQSNIPLYTLETYTPLCAL
                     DMLGFNAACELLYTNILQVIDLGTIPLYAKNRGDSYPLIIIGGEAMSNPAPMLPFADA
                     IFVGDGEEAIIRIAQVLIDKKDKHLLKDEVLTKIAAIEGVLVSRDVEYGYTGNAITAI
                     KAPAVYKRVARRFHDYEVTRPVVPSIRSVQDRVSVQLDRGCKNLCKFCHAGYWELPYR
                     KVEVSRVAQAIVTTLQSTGFNDVSLFSLSVGDYTNIVSLLNIIVPQLIEQGVSISLPS
                     LRVDMQSIAIIESISDLKKTALTFAVESASDALRYRAYKRLTIDEIMNIITELSRRGW
                     QRIKLYFMIGLPGCDDTDEAAAIIAFMKQACSIDKRMRYNVTISPFVPKAHTPFQYEK
                     QYDEHYCLEVVNTIKRNVPKNVTIKNHDVYASLIEGVLARGDSMLADAIAGAYNAGAR
                     LDLWGEHFDFALWDNALQTSCGNWRRYLNRRSDQELLPWSMIQTRYERLIEKKKDCTW
                     NGRGLPHGFKNEILNREAFAQAAQQFTMRYALAATLRMHYAKKGKARFIGHIDSMEIV
                     KRALRMAHIPVSYTQGYNKHERLITGPALPLGYESYSEFVDVQLYESCSAMSLQKLQV
                     SFPDGFEVVNLYHHDASTLPLKRIVFAEYLLQFTDNYRFTLFINRLESKNDIVKTTKK
                     GLMNLRFDDAIAGVIVADSSIRLVMALGEHAIRPDTLVADWCGVHNPLTIAAITKVAS
                     YYLHNDELKKFH"
     gene            9273..10037
                     /locus_tag="PLX22_04250"
     CDS             9273..10037
                     /locus_tag="PLX22_04250"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT19143.1"
                     /translation="MGSLSYNLDGKVAIVTGGTRGIGLAIAQELLKQHAKVVICARKQ
                     EGLDRVKEMLKAGDDLLTCVAHIAKEDDVNALVDAAVQKYSTVDILINNVGMNLMTGS
                     LVDVELSAWQKIIDSNLTGTFLVSKKVGAIMRQQKRGKIVSISSVAGRLASPGMNVYG
                     IAKAGVEMMTKNLAVELAPFNVQVNAVAPGMVRTDFSKPFWSNEAIHDMVVKNIPMGR
                     IAEIDEVVHPVLFLASDGARYITGQTIVIDGGASIL"
     gene            complement(10038..11702)
                     /locus_tag="PLX22_04255"
     CDS             complement(10038..11702)
                     /locus_tag="PLX22_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOT19144.1"
                     /translation="MNLTILLLGVNARYIHSSLPLYYLKQILQPLNYTVYLHETTINQ
                     PVDDTRNAIVTINPDVLCLSVYIWNATYIKKLLPMVHTALPNCIIILGGPEVSYTADE
                     WLKHFPFITTIVQGAGERSFEYAAQKQFLLDTKTVSLPNYHLNEIPFPYSESDINNVA
                     SRFIYYESSRGCPYACSYCISSREDQSIQFRDLSKVYAELDIFLQHRVRMVKFVDRTF
                     NCNPTHARSIFYYLLSHNNNHTHFHFEIHPDLLFDEDFTLLQQVPKNYFQFEIGIQSI
                     HPKTLSSINRKMNWEQAKENIAKLMELNTIHIHLDMICGLPYEDIEAIKESFNEIIEL
                     KPHYFQMGFLKILPGTKIASQKNEFGIEHDAEAPYTVIKNNWIPGHMMQALHCIERVV
                     DRFYNHHFKTTWMMLCHFITSGDSFKSFEMIAQFFYKKNFVLFAKDWEAIATMILTFI
                     KEEYPPVEELAIDCLRWDWFIQSNSKWVPACVRSKTNPNIVKEIIINNHKIAKDELLL
                     DGKRIPVRTIQHAQIFIAESKTFQQWHMAGHHYAIKLHRDTILYSA"
     gene            complement(11771..13090)
                     /locus_tag="PLX22_04260"
     CDS             complement(11771..13090)
                     /locus_tag="PLX22_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOT19145.1"
                     /translation="MTLKNTEIQKAEGKLGVVIPGLGAVSTTFIAGVMLIRKGLKSPK
                     GSVSMMGKLKIGKGDDAHYVPIKDFVPLANLQDIEFAGWDIFEDNAYEAAINAGVLQK
                     EDLEPIKDELSKVKPWKAVFFNEYVKKLHGTWTKKAPSHWELAQMVKEDILNFKKEKN
                     LSRVVMVWCASTEIYQEMKPGVHDTIENFEKALKENNKYIAPSMIYAYAALDLGIPFA
                     NGAPNLCNEIPALRQLAEKNKVPMGGRDFKTGQTLMKTIIAPGLKARMIGVDGWYSTN
                     ILGNRDGEVLDDPESFKTKEVSKLSVLDNIFQPDEYPELYDGYYHVVRINYYPPRRDN
                     KESWDNIDIRGWLDYPMQIKVNFLCRDSILAAPVVLDLVLFFDLAQRATMYGIQEWLS
                     FYWKSPMFEPGEEPIHDLFVQLMKLKNTLRWLMDVPPVTYLGSESYD"
     gene            complement(13382..14830)
                     /locus_tag="PLX22_04265"
     CDS             complement(13382..14830)
                     /locus_tag="PLX22_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HOT19146.1"
                     /translation="MNAPLLIAKGEKDLCIIPRMANRHGLIAGATGTGKTVTLQVIAE
                     NFSLAGIPVFMADVKGDLSGISKPGKPNDKLAQRLKKLKIDNHTFQGFPVVFWDLFGK
                     SGHPIRTTVTDMGPLLLSRILNLNETQTGTLNVIFKIADDNGLPILDIKDLRSMIQFA
                     GDNASKFNTEYGRVSTASIGAIQRSILELQNQGAELFFGEPALNIEDFIQTDETGRGY
                     VNILSAESLINTPKLYATFLLWLLSELFEILPEVGDRDKPKIAFFFDEAHLLFNDAPR
                     ALLEKIEQVVRLIRSKGVGVYFITQNPADIPQTVLGQLGNRIQHALRAFTPNDQKAVK
                     AIAQTFRENSKLDIEKAIIELEVGEALVSFLDEDGKPEIVERAFIVPPKGQIGPITPQ
                     ERNEIIDASPVKGIYDKTIDRESAYEMLKTRAQKAVEATHEKEDIPKQRAGRKSDTVV
                     EAMMKSAARSIGSSVGRQIVRGILGSLLGGKR"
BASE COUNT         4502 a         2953 c         3135 g         4706 t
ORIGIN      
        1 tacctttata gctaactctt ccgaatcaac atcaattgaa atgccgctca tagcctttac
       61 agggcaggct aatttacaca gtccgcatgc aacacagcgt tcaggataaa attccaccat
      121 cattgtttca cggtttatca ccagcgcatc ggttcggcat actgctgtac aggcaccgca
      181 gtgtacacat ctatcctcat cacgctgaat cttatcggct aaaatatcaa caattacttc
      241 ttcatttatc agataattga tacaggtatc tatatcttct tgttcgccgg aaagctgcaa
      301 tataagcctg ccttcctgct tgggcaatat ttttgcctca agcacattaa atattacatt
      361 aaaatcttta gcaatgcgat agataatggg tttatacatt atattctggt taaatattaa
      421 aagcacattt cgtgattcca tgataacctc gtttatgacg tttgagtaag ttctatgcag
      481 cctttcatag ccattgtatc ccagtatatc accagagctc ccgtaacgcc agatattgta
      541 caggcatact ccagggcatg gacacaatcc tcttctgttt gtactctgtt acaggttgct
      601 gttgcaaccg catcagccag tgacgccgac gatgcaagta ttgttacaca atcggctttg
      661 ccaaaactaa atgagtgccc aaaacgccct gatgacgagc aaatacccag tggcgtgtcg
      721 tgcggttgaa taacaatacc acaatcatct gaaaatgatg acctccccgc aaaaacctta
      781 atgacagcag gctcctgtac ctttgcccag atatcaccac cgttttcaat aattatctcc
      841 gggctatagg cagtcagctc ttcgccaatc atctgggcga tagttccggc aacggctgcc
      901 attggaccaa cggaagcctt tgctgaagca ctatacatcc tccgtactac ctccggtgca
      961 gatggatcaa attcaagagg tgatagcgca tgcaggaagt tatcatgttt tgcaatgtag
     1021 tctttaatgt cgttgcgcaa ccaatgaacc aattgcaggg ttctatcgcc aagattgtta
     1081 tgagcacata caaacaggct ggtggtttcg acacaaactt caaatgttgt aaagcgtgta
     1141 tcctgaatca gtgctctgta tgccaatgtg ttgttcataa ctgtatgctc atggtgctgt
     1201 aacagtttag tttactcatg atgtttttgg tacggaacgg ttggcaacag tatctgcggc
     1261 tcgccaaccg taaattgcct ctgcacaatc cagtctttta aaatgtcggc tatttttttt
     1321 gccattggat agcttgaaag cggagctgtt gggatacgct tgccggcaat ttcaacaaat
     1381 cctgatttta actgtgcata gcttacctgg gcaatagatc tgctgatggc atttggatag
     1441 tcataaccgt aatcaaccac ctggcagtaa atagcatcat ccgaaacgcc ggtaaaaaat
     1501 gctaaatcct catttaaaat gggtatgggt atgccaatcc caaccatgag actgcatccg
     1561 taaccggtaa tggatgcacc ttttataaac tgcgttgtca tgtgcttcat atcacctgtg
     1621 acagctattg tgccggcgcc tgatgtaacc acgccgtttt cgcttcgcgg ttcgttgggg
     1681 ctatgctgtg tgccacgcca ggtaactgca cccacactgc cagccagaaa tatctttgtg
     1741 ccgacgccaa tggtccaata gtacggatca tttaacagcg gactaagctg tccggcactt
     1801 gaataggttg cattccccat attgggacgc agtatgccca tataggtgta gatagtcttt
     1861 ttggattgat taactgcgca gttatagttt tgataggcat tgcgcggatt gtataaaaaa
     1921 gcatcgcgca gatcattgat ggttaccgtt gcttcatatt cacggagggg atagcagtcg
     1981 gttccgtaac tttttgcttt taattttaca ggtttgccgg ctattaaatc ttcaataaca
     2041 tgcccaccac cgtatttaaa tctgccggga tgaaccttgt ttaaaggatc atcctcctgt
     2101 acctgtgttg ctcctatgta gatatcaacg gcagcaagcc ctgcgtatgc ttccacatcg
     2161 ttaagccaca cctttgatgc ctttatcttg ggacgggtat gcccaaagtt aataaatgca
     2221 ccgctggaac acattgtgcc aaacgttccg gtggtaacaa cgtcaacctt tttaaatgct
     2281 gatttatacc catgggattt tacaatgctg gtcatttcat cggcagttac aacaacagcc
     2341 tcaccttttt ttatcttatt gtttattgcc tcataacttt tttttatgac aatatcgctc
     2401 attgcatggt tatccttttt gtaatattgg ggtgaatgaa cattatgctt acaatactat
     2461 gagcatgcaa gtaaaaatta tttttttaaa ttttatagca aaaatcccat gggtatggca
     2521 aatggcaaag aagtcctgag tcggttacaa atatctccgc attttttata aattttgtta
     2581 aaaaaacttt acaaataagt ttaaaatagc cataattgta taaacaatat atttgttaca
     2641 ttgtacagat tgttatgtac catgaaaaca tatggaatat aaaacaaaaa ggaaaattca
     2701 atgccacgat taaaacccca tgctcgtgct gaaaaagagg aattacgcaa aacacagatt
     2761 acccagtctg cttatgaggt tatatctgct aaagggtata ataattttac cctggatgat
     2821 attgcaaaaa atgcaggact ttcaaaggga ggtgtactgc actattttaa atctaaagag
     2881 gatatactgc tgtatcttct ggagcagatt tacatcatta tcaagaaaaa tattaataaa
     2941 cgctcttcaa agtatcgtac cccggaacgc aggttacgag caattgttat tgcttttatt
     3001 ataactgcta aacgtcaccc tgcgttttat acagtgctgg ttgacttctg ggcgcagata
     3061 cccatacatg agcgggttaa atatattaat acccagatat ataagcaact ttgtgatgaa
     3121 gtaatcaagg tcattgagga tggtatcgca tcaggtgtat ttaaacaggt acatgctgat
     3181 aatacagctc atgccattgt ggctatggtt atgaatgttg ccatacagtg gacgtttaat
     3241 catgaacttt acaatatcga tcatatggca aagacatgta tgaaaatgat catggcttat
     3301 cttgaagaaa aaactatata aggggtatta ttatacgggc aactgtcttt gatttaaaat
     3361 cagtatataa tgtaaaattg gtgtatggta tcccaacgct ttttaatagt actacaacca
     3421 tggatagccc aagccccatg ccttctgcgg tagggtcagg attttcaaga taaaaatcgg
     3481 ctatgctgtc atagtaagat gaccgttcaa ttctttcacg catgcgttcc agctcttttt
     3541 gaggtaccgg taagttgttg ataacctcaa tggtcaatcg tgttgtttgc ggctttagat
     3601 aaattcgtgt tgataacctg tattgttttg ccttaattcc atagagaagc aacgattctt
     3661 cattaagtat atttctaacc ttattaacca cttccataat atcattggga tctttaatct
     3721 ccccttcttc tataagtata tattttgcat tggctttaat tgcatttgca actaactctt
     3781 ttaaggagga gtaaacagga gtgtacaggt cagatttatt gtggcgttca agtatcttgt
     3841 ttagtgcaaa ctgaatattt ttttctgttt catcaagaat agaatagctg acaaaaacta
     3901 tctcctgatt tctgttgatt gctgataata tttcgtgtac tatgtcctgt gatgttgcct
     3961 gtgccatgat cgtggtataa ttaacttaga atttagttat actatcggta taatgctaat
     4021 aatgcaacaa ttttttaatt gcaacaatat tgacattttt atatactgtg ttacacaata
     4081 ccaccttata atactctgat gaggtacact atgttcaata tgctttttcc atctgtatca
     4141 tctattactg ttataacgct attgagtttg gtatgcggta tattgctttc actggcaaaa
     4201 gtactgctca aggttgaaaa ggacccgcgt attgagaaaa ttgtagaatc acttcccgga
     4261 gccaactgtg gcgcatgcgg ttttcctgga tgcgttggtt atgccacaca tataattgaa
     4321 aagggtgcat ccattaacct ttgccctgtc ggcggtccgg agagtgcaca aaagatatca
     4381 cagattatgg gaatagaagc tgagatagta gcaaaacgta ttgctcgtgt acactgccag
     4441 ggtggtatta acgaaacaaa gatacgattt atgtatgatg gtcctcatag ctgtacggct
     4501 gctcaactgg ttatgggcgg tttcaaggtt tgtgaatatg gatgtttggg gttgggtgat
     4561 tgtgtccgtg catgcctctt tgatgctatc catttagaca aacgagggtt gccattggtt
     4621 gatgcagaaa aatgtaccgg gtgcggaaac tgcgttgaag catgtccgcg taatattata
     4681 agccttttag gtgaaaacat tgatgtttat atgatgtgcc gcaacaaaca aaaagcgccg
     4741 gttatgaagt taggatgcaa tgttggttgc acagcatgcc ggcgttgcga aaaggtctgt
     4801 cgcgaggaag tttttaagga taatcccgat gtggatacag caattaaagt ggttgatttt
     4861 ttagctgtta ttgattatga attgtgcatt aactgcggca ggtgtgccga agtttgtccg
     4921 cagaatgtta tagattttaa aaaagttatg gtaataaagc gtgaaaccaa ttgaccagat
     4981 gtctggtgca gaaagggctg cggcacttct ggtagcatta ggtccagaag ttgcttctga
     5041 gataatgaag tttcttgatg acgagagtat tcaaaaatta gcttcggaga ttgctaaaat
     5101 cgaagttctg tcaccacagg aaaaagaaga tttgatagga cagtttttgc ttgagcttaa
     5161 aaaaactcgt ggtataacat ttggtggcca aaacgtagca aaagaaatat tatatgcagc
     5221 ctttggacag gataaagctc aggatatctt aaaacgatta acccataagg atctggaaaa
     5281 aggttttggc tttttaaatg atattgaccc ggagttaatt gtaacatttt tgcacaatga
     5341 acatccgcag acgattgctg ttacgctggc atacctgccg gctgcaaagg ctgctgaggt
     5401 tataaagctt ttaccaaccg atattgccaa agaggtaact ttacgcatgg caaagatgga
     5461 acgcacttca cctgatgcgg tgcttgagat atcgcgcgtg ctacgcaaaa aatacgatga
     5521 gtaccgatat caggggcaat ccagttcaaa ggcaggtggt cttaatgcgc tcattgatat
     5581 aatgtctcat ttaagtggtg accaggaaaa acagcttatt gattattttg aaaccaccat
     5641 gccgcaaatt gctgatgaga tacgtgcacg catatttaca tttgaaaatg taattaccct
     5701 taccaatact gagatgcgca tcctcattga tgaaatcaat aatgactata tgattgccag
     5761 agcattgaaa ggagcaggtg atgaaataag gtttaagttt ttccgcaaca tgagtaaaaa
     5821 ccgtgcaact gatatcatga atgagatgga agccatgggg cctgtgcgat tatccgaaat
     5881 tcaaagtgct cgcgacaata ttataagggt aatgcgcata ctgcatgaca atggtgttat
     5941 aagcctcaga aaagaaaaag agaagtatat tgaataaaaa agaaatcctt tcatggcttc
     6001 agcaatatgg gcttgctcct aacaaaatgt atgggcagca ttttttgttt catgaaaaat
     6061 atattgaggc gatagttact gcaatcagtc cttgcagcga agatgtgata cttgaaattg
     6121 gtccagggct gggagtgctt acgcaacgat tgagtgaaca tgcaaagaag gtcattgcgg
     6181 tggagattga tgctggattt gttgaggttt tacaatcaag gtttgctaac aattccagag
     6241 taactatcgt acataatgat tttttaaaaa caaatcctta cgatgaatgt actgtactaa
     6301 ccggtaatct tccttataat atagcttctc agattatttt tgcggcagca cataacttta
     6361 cagcacccca ttgttttttt ttgctgcaaa aagaaatggc acagcgtctt atagcaaaac
     6421 caaaaagtgc catgtattca gcatttacgg ttgggattgg attatatttt actgttcaaa
     6481 cattatttga tataccgccg caagcctttt accctgagcc taaggtgtat tcaacatttg
     6541 taaagcttac ccgaaaaaat tctgtaatgc ttcatactga tgaaaaagcg gtgtttgagc
     6601 agcttgtagc aactgcgttc tgggcacgcc gaaagatgct taaaacagcg ttgaaacgtt
     6661 cgccctatac aacctaccgc gaggctttta tttcacaggc ttttggccaa ttgggttttg
     6721 atgaaaaggt tcgtggcgaa gagcttaccc ttgatgaatt tgttgcactg gcaaagtact
     6781 gtgcacacta tggcaataaa taacaagcaa taagtaaatg ggatatgata ccagaagaaa
     6841 caatatataa tctgcttacc actgtgtcaa aaccggcgcg atatgctggt ggtgaatgga
     6901 atgcagttgt aaagcctcat gcacgggtaa aagtgggatt gtgttttccc gacctctatg
     6961 aggttggcat gtccaacaat ggcattcaga ttttgtacca tgccatcaac ggaatgcaac
     7021 atgccgcagc agaacgtgtc tttgccgttg agcatgattt tgaaaaccag ttgcggcagt
     7081 caaacatccc tctttacacg ctggaaacct atacgccgct gtgtgcgctt gacatgcttg
     7141 gctttaatgc agcatgcgaa cttttataca ccaatatttt acaggttatt gatttaggca
     7201 ctatcccttt gtatgcaaaa aataggggcg atagttatcc tttaatcatt attggcggcg
     7261 aggcaatgag caaccctgca ccgatgctac cttttgctga tgcaatattt gttggtgatg
     7321 gtgaagaggc aattatccgg attgcgcagg tgcttattga taaaaaagat aagcacttat
     7381 tgaaggatga ggtgttgaca aagattgctg cgattgaagg cgtactggta agccgcgatg
     7441 ttgagtatgg gtatacaggc aatgccatta ctgcaataaa agcgccagcg gtatataaac
     7501 gtgtggcacg gcgtttccat gattatgagg taacacgccc tgtagtgcct tcaattcgca
     7561 gcgtgcagga tagggtatcc gttcagcttg acagaggatg taaaaatttg tgcaaatttt
     7621 gtcatgctgg ttactgggaa ttgccctatc gtaaggttga ggtaagcagg gttgcacagg
     7681 ctattgttac aacactgcag tcaaccggtt ttaatgatgt atcgctgttt tcgctgagtg
     7741 tgggcgatta taccaatatt gtcagtctgc tcaatatcat tgtgccacaa ctcattgaac
     7801 agggtgttag catatcgtta ccgtcgctgc gtgttgatat gcaatccatt gctatcattg
     7861 aatcaatttc agatttaaaa aagacagcac tcacctttgc tgttgaatca gcatcagatg
     7921 cattgcgcta tcgtgcttac aaacggctta ccatagatga gattatgaac atcattacag
     7981 agctgtcacg gcgtgggtgg cagcgtataa aactttattt tatgataggg ctgccgggat
     8041 gtgacgatac cgatgaggcg gccgctatca ttgcatttat gaagcaagca tgttccattg
     8101 acaaaagaat gcgctacaat gtaactatct ccccatttgt gccaaaagct catactccat
     8161 ttcagtatga aaaacagtat gatgagcact actgccttga ggttgtcaat accatcaaac
     8221 gcaatgtgcc aaaaaatgtc acgattaaaa atcacgatgt gtatgcatcg ctcattgaag
     8281 gtgtcctggc acggggcgac agtatgcttg ccgatgccat cgctggtgca tacaatgctg
     8341 gtgctcgcct tgatttgtgg ggggagcatt ttgattttgc actgtgggat aatgctttac
     8401 aaacaagctg tggcaactgg aggcgctatt taaataggcg gtcagatcaa gagcttttac
     8461 cctggagtat gatacaaacg cgctatgaaa ggttaataga aaaaaagaag gactgcacat
     8521 ggaatggcag gggtttgcct catgggttta aaaatgaaat tcttaacagg gaagcgtttg
     8581 cacaagctgc acagcaattt accatgcgtt atgcgctggc agccacattg cgtatgcatt
     8641 atgcaaaaaa aggaaaagcc cgttttattg gacatattga tagcatggaa attgtaaagc
     8701 gagcgttgcg tatggcacat attcctgttt cgtatacaca gggatacaac aaacacgaac
     8761 ggttaataac agggccggcg ctgccgttag ggtatgaaag ttattcagag tttgttgatg
     8821 tacagctata tgaaagttgt agtgcaatga gcttgcagaa gttacaggta tcatttcctg
     8881 atggatttga agttgtcaat ctatatcacc atgatgcatc aacgctgccg cttaaacgca
     8941 ttgtctttgc tgaatacctg ctacagttta ccgataacta tcggtttacc ttgtttatca
     9001 accgtttgga aagcaaaaac gacattgtaa aaacaacaaa aaaaggattg atgaatttgc
     9061 gctttgatga tgcaatagcc ggtgttattg ttgctgatag cagtataagg cttgtcatgg
     9121 cgctgggtga gcatgcgata cgccctgata cgcttgttgc tgattggtgt ggtgtacaca
     9181 atccattaac tattgctgca attacaaagg ttgcatcgta ttatttgcat aatgatgaat
     9241 taaaaaaatt tcattaatat acgaggtatg ctatgggttc gctttcatac aacttagacg
     9301 ggaaggtagc tattgttacc ggtggtacac gaggcattgg attggctatt gcgcaggaat
     9361 tgcttaaaca acatgcaaaa gtagtcatct gcgctcgtaa acaggaaggg cttgacaggg
     9421 taaaggagat gcttaaagca ggcgacgacc ttttaacctg tgttgcacac atcgcaaaag
     9481 aggacgatgt caacgcactg gttgatgccg cagtgcaaaa atattccact gttgacattt
     9541 taattaataa cgtagggatg aaccttatga ctggtagcct tgttgatgtt gagctttctg
     9601 catggcaaaa gattattgat tcaaacctta ccggtacatt tcttgtttca aaaaaggttg
     9661 gggcaattat gaggcagcag aaacgcggaa aaatagtcag tatatcttca gttgccggca
     9721 ggcttgcatc gccgggaatg aatgtgtatg gtattgctaa agccggtgtt gagatgatga
     9781 caaaaaattt agcagtggaa cttgccccct ttaatgttca ggtaaatgca gttgctcctg
     9841 gtatggtgcg taccgatttt agcaagccat tctggtcaaa tgaagcaatt cacgatatgg
     9901 tggttaaaaa tattcccatg ggaagaattg ccgaaattga tgaggtggtg catcccgttt
     9961 tgtttttagc ctctgatggc gcacgctata tcaccggtca aactattgtg attgatggcg
    10021 gagcttcaat actatagtca tgcactatag agtatagtat cccgatgtag ctttattgca
    10081 taatgatgac ctgccatgtg ccattgctgg aaggttttgc tttcagcaat aaaaatctgt
    10141 gcatgttgta ttgtgcgtac gggtatacgc tttccatcaa gcaacaactc atccttggct
    10201 attttatgat tattgatgat tatttcctta acaatgtttg gattagtttt gctgcgtaca
    10261 catgcaggta cccatttgct atttgattgt ataaaccaat cccatcgcag gcagtctatt
    10321 gcaagttcct ctacaggggg atactcttcc ttaataaagg taaggatcat agttgcaatt
    10381 gcttcccaat cctttgcaaa caacacgaag ttttttttat aaaaaaattg cgcaatcatc
    10441 tcaaaacttt taaaactatc gccgctggtt ataaaatgac atagcatcat ccatgtagtt
    10501 ttgaaatgat gattataaaa cctgtcaacc acacgttcaa tgcaatgaag agcttgcatc
    10561 atatggcctg gaatccagtt attcttaatg acagtatagg gcgcttcagc atcatgctca
    10621 ataccaaatt catttttttg tgatgcaatc tttgtgccgg gtaaaatttt taaaaatccc
    10681 atctgaaaat aatggggctt taattcaatg atttcattaa agctttcttt gattgcctca
    10741 atgtcttcat acggcaaccc gcatatcatg tctaaatgga tatgtatagt gtttagttcc
    10801 ataagctttg ctatattttc ttttgcttgt tcccagttca tctttcggtt tatgctgctc
    10861 agtgtttttg ggtgaatgga ctggatacca atctcaaatt gaaaatagtt tttgggtacc
    10921 tgttgtaata atgtaaaatc ttcatcaaac agcaaatcag ggtgtatttc aaaatggaaa
    10981 tgagtatggt tattgttgtg cgatagtaaa tagtaaaaaa ttgatcgagc atgggtggga
    11041 ttgcaattaa atgttctatc aacaaacttc accatcctga ccctgtgctg tagaaaaatg
    11101 tcaagctcag catacacctt tgataaatct ctgaactgta ttgattgatc ctcccttgaa
    11161 gagatgcaat aactacacgc atacgggcaa cccctgcttg attcatagta tataaaacgg
    11221 gatgctacat tgttaatatc actttcagaa taggggaagg gtatttcatt aagatggtag
    11281 tttggtaatg atacagtttt tgtgtctaaa agaaattgct tttgtgcagc gtactcaaat
    11341 gatcgttcac ctgctccctg tacgatagtt gttataaatg ggaaatgctt cagccattca
    11401 tcagctgtgt agctcacttc aggtccaccc agtattatta tacagtttgg caatgctgta
    11461 tgtaccatag gaagcaattt ttttatgtaa gttgcattcc atatatacac tgataaacac
    11521 aacacatccg gattaatagt aactatcgca ttgcgtgtgt catcaacagg ttggttaatg
    11581 gttgtttcat gaagatatac cgtataattg agtggttgta aaatttgttt taaatagtaa
    11641 aggggcagcg aggaatggat atatcgtgca ttaacgccaa gaagtaaaat ggttaaattc
    11701 atataataaa aaataatttt gtaaagaaaa tcccggtggt gtcaccagga ttttcttctg
    11761 tattattata tcaatcatat gattctgaac caagataggt aaccggtgga acatccataa
    11821 gccagcgcag ggtattcttt aatttcataa gctgtacaaa caggtcatgg attggctctt
    11881 caccaggctc aaacatgggg cttttccagt aaaatgatag ccactcctga atgccataca
    11941 tggttgcacg ttgagccagg tcaaagaata gtacaaggtc aagtacaacc ggtgcagcca
    12001 gaatagaatc acggcataaa aagttaacct ttatctgcat ggggtaatcc aaccagccgc
    12061 gtatatcaat attgtcccag ctttctttat tgtcacgacg tggaggatag taattaatgc
    12121 gaacaacatg atagtagccg tcgtacaatt ccggatattc atcaggctgg aatatattat
    12181 caagaaccga aagcttgcta acctctttag ttttgaatga ttcaggatcg tccagtacct
    12241 cgccatcacg gtttcctaaa atatttgttg aataccagcc gtcaacgcca atcatccttg
    12301 cctttaaacc aggggcaata attgttttca ttaaagtctg ccctgtttta aaatcccgtc
    12361 cacccatagg caccttgttt ttctcggcaa gctgtcgtaa ggcaggaatt tcgttacaga
    12421 ggttgggagc gccgtttgca aaaggaatac ctaaatccag cgctgcatac gcgtatatca
    12481 tgcttggggc aatatactta ttattttctt tcagcgcttt ttcaaaattt tcaatggtat
    12541 catgaacacc tggtttcatt tcctgataaa tctcagtgct ggcacaccat accataacaa
    12601 cacgagataa gtttttttct tttttaaagt taaggatatc ttctttaacc atctgtgcta
    12661 attcccagtg tgaaggagcc tttttagtcc atgtcccatg gagtttttta acatattcat
    12721 taaagaaaac ggctttccac ggctttactt ttgatagttc atctttaatg ggttcaagat
    12781 cttccttttg cagtacacca gcattgatag ctgcttcata tgcattatct tcaaaaatgt
    12841 cccatccagc aaattcaata tcctgcaaat tggcaagtgg aacaaaatcc ttaatgggaa
    12901 cataatgtgc atcatcaccc ttgcctatct ttaactttcc catcatcgaa acagaaccct
    12961 tgggtgattt taatccttta cggataagca ttacacctgc aataaacgtg gtgctgacag
    13021 caccaagccc gggtataaca acccctaatt taccttcagc tttttgaatc tcagtatttt
    13081 taagtgtcaa ggtacgcctc gcttttaatt taaaaaattt atgtaaaaaa taataacatc
    13141 ctgcaataga acaaaaaaac atgctattta ttacaaatta ctaaacataa ctatcatatc
    13201 catagtcaag aaatttatcg aaaaaaggca gattattaat aacaataaaa caataatccc
    13261 agatggttgg taatatattt ttttgccatt aaacaaacat tattataaat gtcaatagaa
    13321 aaaaatgaca tgatgtttga aaagtaaaag gcatcatgaa aggtaaataa aacagtaata
    13381 actatcgttt acctcctaat agtgaaccta aaattcccct tactatctgc cgtccaacag
    13441 agctccctat tgaacgagca gcacttttca tcatagcttc aactacggta tcactttttc
    13501 taccagctct ttgtttaggg atatcttcct tctcatgagt agcttctaca gctttttgtg
    13561 cacgagtctt cagcatttca taagctgatt ctctgtctat tgttttatca taaatgccct
    13621 taaccggtga tgcatcaata atttcatttc gttcttgcgg cgtaatggga ccaatctgtc
    13681 cttttggtgg tacaatgaat gcacgttcca caatctctgg tttaccatct tcgtccagaa
    13741 atgacactaa cgcttcgcct acttctaatt ctataatggc tttttcaata tctaattttg
    13801 aattttccct gaatgtttgg gctattgctt tcactgcttt ttgatcgttg ggagtaaaag
    13861 cacgtaaagc atgctgaatc ctgtttccta attgacccaa tacagtttgt ggtatatctg
    13921 cagggttttg ggtaatgaaa tatacaccaa caccctttga acgtattaaa cgaactacct
    13981 gctcaatttt ttccagtaaa gctctgggtg cgtcgttaaa aagcagatgt gcttcatcaa
    14041 agaaaaaggc aatctttggt ttatcccgat cgccaacttc aggtaaaatc tcaaaaagtt
    14101 ctgataaaag ccatagtaaa aatgtagcat agagctttgg agtgtttata agcgattcag
    14161 cagatagaat atttacataa ccccgccccg tttcatcagt ctgaataaaa tcttcaatgt
    14221 ttagtgcagg ttcaccaaaa aataattcag cgccctgatt ttgcagctct aaaatactac
    14281 gctgtatagc tccaatactt gcagtggata cccgtccata ctctgtatta aattttgaag
    14341 cattatcgcc agcaaattga atcatggatc gtaaatcttt aatatccaga ataggcaatc
    14401 cattatcatc agcaatttta aatattacat tgagcgttcc ggtttgcgtc tcgttaagat
    14461 tcaagatacg gcttaaaagg agcggtccca tatcagtaac agtggtacga ataggatgtc
    14521 ctgatttccc aaacaaatcc cagaaaacaa cagggaatcc ttgaaaagta tgattatcaa
    14581 tcttaagctt tttaaggcgt tgtgcaagtt tatcatttgg ctttcctggt ttacttatcc
    14641 ccgaaagatc accttttaca tctgccataa acaccggaat gccagctaaa ctaaaatttt
    14701 cggctataac ctgtaacgta accgttttac cggtaccggt tgcaccagca attaatccat
    14761 ggcggtttgc catacgtgga atgatacaaa ggtctttttc acctttagca atgagtagtg
    14821 gggcattcat ttctttcctc caatgtgtct ttatcattgt ttgtagtatt acctgtacga
    14881 tagttactgt caattctatt ttgctgtttc acatatcata ggctgtaata attagatttt
    14941 gagggtgccc ctgccggaac aggtattgga aaatttttct tgttgatgta tgcaaccaaa
    15001 actttattta ttttctatct ttgtttcaca agagaaaaag ggtctttatg cgcctgcatt
    15061 caaagacgac gtgccttccg tggcacggct gtttattgtt gtgatgatgg cagtatcatt
    15121 ctgaaggagc gccagcgact gaaaaatcta tttttcaatt ttaaaaaaaa tcgagatact
    15181 aatcatccgg ctcaggatga cagacgttaa ctttttcaaa agggattaag atttttcact
    15241 atgctcaaga tgttatactc tgcactttta ttcaaaaaag catctccata tttttt
//
LOCUS       DAOSOX010000036        15040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_3987_length_15040_cov_8.585986, whole genome shotgun sequence.
ACCESSION   DAOSOX010000036 DAOSOX010000000
VERSION     DAOSOX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_15040_cov_8.585986"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2258
                     /locus_tag="PLX22_04270"
     CDS             <1..2258
                     /locus_tag="PLX22_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19147.1"
                     /translation="FAPFRWFFTAKAKGTQFSEKHALMLKERMSSVAVLFSLGLIGQW
                     TIGLAVFSITSALLYGLTFDQLLYLWFGGILSVGISIPFGSYFISKTIKRLTDDGLFD
                     EVKNVISVKPVTTFGSLIPILTGSTSMVNVLLVIVLVTFGIVLSHGYMKTMFAQNINS
                     ISNQAAHKLDDMIDGVIIDCKLVSLLPDRNMINRIVSLKKYYADYAIIENGNVTTSGT
                     GRYDAIIEKGMLSRKSLGQDGEFITKPIVYEKDKKAYVVIGDTINGRIYAGLIAVDAI
                     KEQIFKDVTIGRTGYIFVTYEDGSGLVHPQIDITSDALTHYEWSKKLINNHNTLIAYK
                     FNNDDNYGYCTKSGRGFLLIANIPKSELNEYSKLLALLSLGVGLFWCVVATVILYRTN
                     RGHLEPLKNAREVISKVAQGDLTQNLDIIKGDEVGILSSAVNILIKELREAIAHIIRI
                     SDELASSSTQMSQSITSFADNVQNEASTVEEISATVEEISAGMENVARRADGQNQSLS
                     LLINLLNNLSTTIQTVADTMNASVKEVELIADRSREGSQSLNQMNSTMQVLSKSSADM
                     KNIVKIINDISEQINLLSLNAAIEAARAGDFGRGFAVVADEISKLAEQTAQSIKEIDN
                     LIKQNSTEMTKGTQNITQAIQNIGFIINGVKDISTHIEHVASEMKEQLAINTNVQNMI
                     ADVQSGSEEIKNATEEQRVAILEISRSITNINDLSQSNAGAAEEMSANATNVESMAVQ
                     LKEVMAFFKIS"
     gene            2571..3620
                     /locus_tag="PLX22_04275"
     CDS             2571..3620
                     /locus_tag="PLX22_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOT19148.1"
                     /translation="MSLSLGIAGYQQSGKKTLLQLLTGNSFDMKKGLGVATIHDQRFR
                     TLVDIYKPRKVAPATINISLIGDIDDDIIKEGSIFNTIATMDALCVVIRAFTSDSVYH
                     IKGSVDPFRDYDKLISEFVLHDMMFAEMRINRLQQDKKKTAQQKEHEIALLNKFLQHL
                     ESGTLLNGLQLSNDEIAIIKSYPFITLKQLIIVFNTNDNDSGIINKFKAMFPEHAVYA
                     IAIPVKLENEINELASEKERMEFYASLNIDAPAINLLCNAMIHALGLQSFFTVGQDEV
                     KQWLIPEGITAPVAAGYIHSDIQRGFIRAELMHYDDFIVAGSEELLKKQGKFHLAGKD
                     YIMNDGDIVSFRFNV"
     gene            3635..>4344
                     /locus_tag="PLX22_04280"
     CDS             3635..>4344
                     /locus_tag="PLX22_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOT19149.1"
                     /translation="MNDILKELTHIRELYSETGAVDTIITNEFVTCIYNFFDSNYRNL
                     PWRNTSNAYYILVSEIMLQQTQVSRVIDKYKEFIKIFPTIHALADADLYDVLSAWQGL
                     GYNRRAKYLWECAKAIILQYNGLIPHDIMELQKLPGLGKATAASVAIFAYNIPAIYIE
                     TNVRTVYIYSFLYDTAKVSDAAIALLLQQTMDRLNPRKFYNALMDCGTFIKQHYNASR
                     HSEQYTIQSPFNSSLRKVR"
     assembly_gap    4345..4444
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4445..5164
                     /locus_tag="PLX22_04285"
     CDS             <4445..5164
                     /locus_tag="PLX22_04285"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOT19150.1"
                     /translation="RSWTYLDLTALLSPEWSWTLLSSHSYEFDRNNDPKSDVEKKTFF
                     YEVFTGPTYTMKFGNLTVKLPLWYYYQGFPIRSAVNPSDHDKYYYSHNIEFMPTLEYK
                     IGNFKFWNRVIFHNKVYSSLYDSSSKNKGYSLLIREYFRVEYNVTDQFAVMIGDEIFQ
                     GIIEDDDTKDITPSPLSPGFEYGGFTQNRLYIGFSYKCTPTIIITPMYMYQTQYFDAG
                     NELTEKDHYLFTTLTFIFKTY"
     gene            5315..6088
                     /locus_tag="PLX22_04290"
     CDS             5315..6088
                     /locus_tag="PLX22_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19151.1"
                     /translation="MSFDSLQKLFEFDALLHMVIEPASRFEPVNSRLVDCIEVYKPRV
                     IVKIGLGNPAYIESIMEHSKALVVVVEPSVTVLQSFYNAYGNKQWMNRLYTIAGNFSQ
                     FPVDYYKADMIICIDCLDLLDTGRAIDEFRRSLQFEGVFFYAGFVLPNEDAEGIFDEI
                     AHVINPLHTDYYLEDDLKTFMTLNEFGIVKLNTQKINMNILLLADYLQTRSSELEKLI
                     EEHKDTLTALYDLHSDGTVVLPYMIAAFIRQKPSQEEAL"
     gene            complement(6070..7368)
                     /locus_tag="PLX22_04295"
     CDS             complement(6070..7368)
                     /locus_tag="PLX22_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT19152.1"
                     /translation="MQSVSLVKYSKSTDSLKQAIELCDGFSKLKPGDKVLIKPNLVAW
                     DELFPPAPYGVFTTTRMVEDLIIILKEYGCNDITIGEGSVEVKKGVGTMAAFAGLGYT
                     DLASHYGVKLVDFNESKSEKCSIDASTHLMIAKEALECDFFINFPVLKTHGQTKISLG
                     LKNLKGCLKASSKKLCHHPQLNLEYCFPFVADYVKPELTIIDGIYALEKGALHFGNAY
                     KKDIIIASTDILAADIIGAKVMGYEPSDIAHFVTYATRHNKSLVLSDYAVKGEKLEDH
                     IQPLKWDWSWTADNTGPGAFAKMGIQGVALPKYDDTLCSGCSPIANMCNILVMSAFKG
                     QPLPKVEILNGKKMQARPGYDKTVLLGNCIIKANKNNPTIKESVEVKGCPPNFDDVVK
                     ALKTCGLEVNEMAYLGYMKQQSEKYNGKEGYDPLYYRASS"
     gene            complement(7401..7568)
                     /locus_tag="PLX22_04300"
     CDS             complement(7401..7568)
                     /locus_tag="PLX22_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19153.1"
                     /translation="MVQVDIVWSYAFGASFAAVAAHQLKIVKHFYKLWNCIFTFAIFK
                     KVLHIYHDYFL"
     gene            complement(7582..8403)
                     /locus_tag="PLX22_04305"
     CDS             complement(7582..8403)
                     /locus_tag="PLX22_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19154.1"
                     /translation="MHTCLKWYIPLFVIVVCIPVYDSAVSSQNQITCLHEDWTECASN
                     DIKSKWIEYAPDWKLLKKKDDVLLYTRKLPSGIVQVKAEKIMYVKPEVVEQLVRDVPA
                     YKEFMYKCFIAETIKIFNDEDMIIHNATAMPWPLKPRDGVVRTQVTKDWDYGRFEVLC
                     YGLLPPESEQYVPQVEGFVRMYEVSAYMIAHILQREKVKCFYIIHADPTGVPLFAVNH
                     LIDSYPYYTIIKFEEMLKKPEYIDRGKNSKYLAQIEAFCKQKQSALPKTNQHILP"
     gene            complement(8569..8757)
                     /locus_tag="PLX22_04310"
     CDS             complement(8569..8757)
                     /locus_tag="PLX22_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19155.1"
                     /translation="MKMNGITASFKTLYVILVIVTLTAGSIALSGCDNYDIDESAVQK
                     IDCPDEYSVLPWQWDLKI"
     gene            9038..10279
                     /locus_tag="PLX22_04315"
     CDS             9038..10279
                     /locus_tag="PLX22_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19156.1"
                     /translation="MKARISISLFLLGLGGQIAWAIENQFFNTFLYDRIIPDPKYVSI
                     MVALSAVTATVTAIIMGAFSDSIGKRKPFLLYGYLAWSVSIWVIPMAEWIKPVIVAAW
                     MVIILDSVMTFFGSTSYDAVYNAYLTDVTTDANRGKAQGLLSLATWIALLLVYGASGQ
                     IVESMGYFFFFGAAGALVLLSGVIGGIAAVEPQRAIVTDSKSIVKRIRGSLNMQALRH
                     NKNLLYVLLAIGFWGMAFNVFFPYLLIYLKHFLKIDIATSSVIIAVSIFIGGIAASIP
                     AGIIADKIGRKPVAIFAVILISFSLIAFSYARLPIAIGILATLWIIAQTIWTVATGAW
                     SKDLFPEERRGEFAGYFTLFYVAFTMIPGPLIGAIVADRWGIRATIDGKPGIIPTPEI
                     FVVSAVLILITIIPLLFAQEQ"
     gene            complement(10291..10914)
                     /locus_tag="PLX22_04320"
     CDS             complement(10291..10914)
                     /locus_tag="PLX22_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19157.1"
                     /translation="MKKFIFILSVVTLFTIALYFNVSLTHAQSNSIVFLSPEFTKFVC
                     DEWNRSELTQKLGTKAVGGNDWILTKNKYTGQVRNKQVFAMSRRDCDSMPKVQLTIEN
                     KDGRAICTYGGALVEEYKTVEWAFAPTTVQWYKFASGVWGYMQMPGIMKGFRGPMFVA
                     RANIDNFGIFWKVVGKAAKQINADYKAPCALSQDDIDAIEKYIKNTQ"
     gene            11394..12518
                     /locus_tag="PLX22_04325"
     CDS             11394..12518
                     /locus_tag="PLX22_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT19158.1"
                     /translation="MKRFIMYSIAGIILVIAVSISYLIITEYILIRFPVLVQTNPKIT
                     FIIGDSYFREDEKAQWKIAVVGQTLYQGYWVKTDPDSRMDIRFHDNTAIQLSESSEMA
                     IDSLTVKKLGLQIHTGSMYGKFDKLFKEHDLHVKTPTAFAAIRGTDLGFMVVPESYGN
                     DSLMGQQKKSAYNKGKNKGQTLKTKGATQNPDSGEADLQTLVYSLSGITEIYNPIFDD
                     QKVLLSYRNKVIIGEESTPTNPEELSEDEIKTLQEKLNSIHFDEVLLITDKITFNFGS
                     AEILPSSYPELDKIVEILKQKKVRVRIEGHTDDQGDAAFNQQLSEKRAEAIKNYLVTK
                     GLDPERFETVGFGESKPIADNKNEVGRSQNRRVEFLIIEK"
     gene            12576..12658
                     /locus_tag="PLX22_04330"
     tRNA            12576..12658
                     /locus_tag="PLX22_04330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12610..12612,aa:Ser,seq:tga)
     gene            12665..12751
                     /locus_tag="PLX22_04335"
     tRNA            12665..12751
                     /locus_tag="PLX22_04335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12699..12701,aa:Ser,seq:gct)
     gene            12753..13199
                     /locus_tag="PLX22_04340"
     CDS             12753..13199
                     /locus_tag="PLX22_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19159.1"
                     /translation="MGKLHYYSLQIAKYSKKLYAQFMHYYAVVKQFCFEHKQQIIASI
                     AYIPYIGWLYPLYACEGDGVIQKHAKRGLLYAGVFVFAALVLFLLSFILPRSWRLLSF
                     IVTIVLYINYFIYYAIVIIHIFNIWKGKDFSIPKFEIYLEKIEQYI"
     gene            13225..13298
                     /locus_tag="PLX22_04345"
     tRNA            13225..13298
                     /locus_tag="PLX22_04345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13259..13261,aa:Arg,seq:acg)
     gene            complement(13398..13886)
                     /locus_tag="PLX22_04350"
     CDS             complement(13398..13886)
                     /locus_tag="PLX22_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19160.1"
                     /translation="MNFETTTCISSNHIIVLKHYAKILDVSVSKLISYLIQYAAKKEK
                     RKTQVFKNISYRDRNTDIKWIRFHLCISWSEYEFLLDIKKIWKVSIAKAIEFCVDTVL
                     DEFFNYVIHRRKESLTDNYLSYYENRSYTFDFFKVKGIHCCQFYWGPPPEILKINYGD
                     LH"
     gene            complement(14078..>15040)
                     /locus_tag="PLX22_04355"
     CDS             complement(14078..>15040)
                     /locus_tag="PLX22_04355"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOT19161.1"
                     /translation="ISLHFTNYDFGGERYLKTGETLPEGAIDELKKHDAIFLGAIGHP
                     DVKPGILEKGILLALRFELDQYINLRPIKLYPNVETPLKDKGPEHIDYIVVRENSGDV
                     YTGIGGVTMKGTPHEEAMQIMIYNRFQVERCLRYAFEYARKRNKRKTLALVGKTNVLT
                     YVYDLWERAFHEIGQKEYPDIKRDYYHVDATCMWMVKNPEWFDVLVTGNMFGDIITDL
                     GAMTQGGMGIAAGGNINPEGVSMFEPIGGSAPKYTGKNVINPLAAICAAQMMMEHLGE
                     EKTAARIEKGVIDVLQRDMKSLAAGKMGLTTTQVGDKVAEYAVS"
BASE COUNT         4660 a         2630 c         2888 g         4762 t
ORIGIN      
        1 tgtttgctcc cttcaggtgg ttttttactg caaaagctaa aggaactcaa ttttcagaaa
       61 agcatgctct tatgcttaag gagcgtatga gcagtgtagc agtgctgttt tctctgggat
      121 tgataggtca atggacaatt gggcttgctg tatttagtat taccagcgca ttattgtatg
      181 ggcttacctt tgatcaatta ttatacctgt ggtttggcgg tatactatcg gttgggattt
      241 ccattccttt tggatcgtat tttatttcaa aaacaataaa acgattaacc gatgatggat
      301 tgtttgatga agtcaagaat gtgatcagtg taaaacctgt aacaacattt ggctcactta
      361 ttcctatact gacaggttct acaagtatgg tcaatgtttt gctggttatc gtgctggtaa
      421 catttggcat agtactatcg catggctaca tgaaaaccat gttcgcacaa aatatcaata
      481 gcatatccaa tcaagcggcg cataagctgg atgatatgat tgatggagtt atcattgatt
      541 gtaaactggt atcgttgtta cccgatagaa acatgataaa ccgtattgta tccttgaaaa
      601 aatattatgc tgattatgcc attattgaaa atggtaatgt tacaacatcc ggaacgggac
      661 gatatgatgc aattattgaa aaggggatgc tatcaaggaa atcattgggt caggatggtg
      721 aatttattac aaagccaatt gtatatgaaa aggataaaaa ggcatacgtt gttattggtg
      781 acacaataaa tggaaggata tatgctggtt tgattgcggt tgatgctatt aaagagcaaa
      841 tctttaagga tgttaccatt ggaagaactg gttatatatt tgttacctat gaggatggga
      901 gtggtttggt acatccacag attgatataa ccagcgatgc gttaactcat tatgaatgga
      961 gtaaaaaact cataaataat cataatactt taatagcata taaatttaat aatgacgaca
     1021 actatggata ttgcacaaaa agtggcaggg gctttttatt aatagcaaat atccccaaat
     1081 ctgagctcaa tgaatattca aagttgctgg cactgctatc attaggggtt ggtttattct
     1141 ggtgtgttgt tgctacagtt atattataca gaactaaccg tggccattta gagccattga
     1201 agaatgcgcg tgaggtcatt agcaaggttg cacagggtga tcttacacaa aatctggata
     1261 ttataaaggg tgatgaggtt ggcatattat catctgctgt aaatatattg attaaagaat
     1321 tgcgcgaggc gatagctcat attatcagga tatctgatga attggcttca tcatcaacac
     1381 agatgtcaca gtcaattaca tcctttgccg ataatgttca gaatgaagca tcaacggtag
     1441 aggagatttc tgctacagta gaagagatat ctgcggggat ggaaaacgtt gcccgcagag
     1501 ctgatggaca gaatcagagt ttatcattgc tcataaattt attgaataat ctgtcaacaa
     1561 cgatacagac agtagcggat actatgaatg catcggtaaa agaagtagag cttattgccg
     1621 atagatcccg cgaagggagc caatcgttaa atcagatgaa cagcacgatg caggtgttat
     1681 caaaaagctc tgcagatatg aaaaacattg tgaagattat caatgatatt tcggaacaga
     1741 ttaacctgct atcgttaaat gcagctattg aagcggcacg agccggtgat tttgggcgtg
     1801 gttttgctgt agttgcggat gaaatatcaa agttagcaga gcagacagct cagagcatta
     1861 aggagataga taatttaatt aagcaaaaca gcactgaaat gacaaaagga acgcaaaata
     1921 ttacacaggc tattcagaat attggattca tcatcaatgg ggtcaaggat atatcaacac
     1981 atatagaaca tgttgcatct gaaatgaaag agcaattagc tatcaacacc aatgtacaaa
     2041 atatgatagc cgatgtccaa tccggttctg aagagataaa aaatgcaact gaagagcaac
     2101 gtgttgctat acttgaaatt tcccgctcta ttactaatat aaatgatcta tcgcagagca
     2161 atgcaggtgc tgccgaagag atgtcggcaa atgctaccaa tgttgaaagc atggcagtac
     2221 aattaaagga agtaatggca ttttttaaaa tctcgtaatt ttattggttg ctcaaaagtt
     2281 gctactagcc gcattgatgt tgatccaacg gggtggaact atctggcgat agttttcgcc
     2341 gaactccgat tcaggatatc aagttaatga ttgagattcc ggatcaggtc cggaatgaca
     2401 gggtgataac atgtcattct gaggcgaagc caaagaatct cgtcgattgg agaaagaata
     2461 gattcttcgg tcgctaccgc tccctcagaa tgacaatggg gcaaaagttc ccttcgtctt
     2521 gcgaaaggga ggggttatgg gtaattcaat tttcaatgga gtgaaacaca atgagcttat
     2581 cattagggat tgcagggtat caacaaagcg gtaaaaagac attactacaa ctgttaacag
     2641 gtaattcgtt tgacatgaaa aaggggttag gcgttgcaac cattcatgac cagcgatttc
     2701 gtacacttgt tgatatatat aaaccccgta aggttgctcc tgcaactatt aatatttctc
     2761 tgattggtga tattgacgat gacattataa aagaagggag tatattcaat actatagcaa
     2821 caatggatgc attgtgtgtg gtcatacgtg cttttaccag tgatagtgta tatcacatca
     2881 agggtagtgt agatccattc agagactatg ataagcttat cagcgagttt gtgctgcatg
     2941 atatgatgtt tgctgaaatg cgcataaacc ggttgcaaca ggataaaaag aaaacagcac
     3001 agcaaaaaga acatgaaata gcattgctta acaaattttt gcagcatctg gaaagtggta
     3061 cactactgaa tggcttgcaa ttatcaaatg atgaaattgc tattataaaa agctatccct
     3121 tcattacctt aaaacaatta attatagttt ttaatactaa cgataatgat tcaggtataa
     3181 taaacaaatt caaagcaatg tttccagaac atgccgtgta tgccattgcc atacctgtaa
     3241 agctggaaaa tgagataaat gaattagcca gtgaaaagga acgcatggaa ttttatgcat
     3301 cgttaaacat agatgcgcct gccataaacc tgttatgcaa tgcaatgata catgctttag
     3361 gcttacaatc attttttaca gtagggcagg atgaggtaaa acagtggctt atacctgaag
     3421 ggataactgc accggttgct gcaggatata ttcatagcga tatacaacgg ggatttatac
     3481 gggctgagct tatgcactat gatgatttta ttgttgcagg ctctgaagag ttattaaaaa
     3541 aacaggggaa atttcattta gctggtaaag actatattat gaatgatggt gatattgtat
     3601 catttcgctt taatgtatag gcggcgcctg aatcatgaat gatatattga aggagcttac
     3661 tcatatacgg gaactatata gtgaaacagg cgcggtggat accattatca ctaatgaatt
     3721 tgtaacatgc atttataatt tttttgatag taactatcga aaccttccat ggcgcaatac
     3781 cagcaatgcg tattatatat tggtatcaga gataatgtta cagcaaactc aggtttcccg
     3841 tgtcatagat aaatataaag agtttatcaa aatatttcca acaattcatg cgcttgcaga
     3901 tgcagactta tatgatgtgc tttcagcatg gcagggattg ggttataacc gccgtgcaaa
     3961 atatttgtgg gagtgcgcaa aagccattat attgcagtat aatggattaa taccgcatga
     4021 tataatggaa ttgcagaaac tgcctggtct ggggaaagca acagcggctt cagttgctat
     4081 atttgcgtat aacataccag cgatatatat agaaactaat gtacggacgg tgtatatata
     4141 ttctttttta tatgatacag caaaggtcag tgatgcagca attgcgttgt tgctacaaca
     4201 aacaatggat aggctcaatc cgcgtaagtt ttataatgca ttgatggatt gtggaacttt
     4261 tataaagcag cattataatg cctcacgcca tagcgaacag tatacaatac aatcaccttt
     4321 caatagttca ttgcgtaagg ttcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnncgttcg tggacgtatt tagatttaac agcattactt tcgcctgaat ggtcatggac
     4501 attgttatca agccatagct atgagtttga tcgcaataat gatccaaaga gcgatgtgga
     4561 aaaaaagact tttttttatg aagtttttac cggtcctact tataccatga agtttggtaa
     4621 tttaacagtt aaattgccat tatggtatta ttatcaagga ttccctatac gttctgcagt
     4681 taatccatct gaccacgata aatattacta ttcacataat atagaattta tgccaacatt
     4741 ggaatataaa attgggaatt tcaaattttg gaacagagtc atttttcata ataaggttta
     4801 ttcgtcatta tatgattcaa gttcaaagaa caaaggatat tcgctactaa ttcgtgaata
     4861 tttcagagtt gaatacaatg ttaccgatca gtttgccgtt atgataggcg atgaaatttt
     4921 ccaggggatt attgaggatg atgataccaa ggatataaca ccttcgccac tttctcctgg
     4981 ttttgaatat ggtggtttta cacaaaacag attgtatatt ggattcagct ataaatgtac
     5041 accaaccatt attattactc ctatgtatat gtatcaaaca cagtactttg atgctggaaa
     5101 tgagttaact gaaaaggatc attatctgtt tacaacatta acgttcattt ttaaaacata
     5161 ctgatgcaat acagcccacg ggttaaaccg tgggctgtat tgctttacgg ttggttgtac
     5221 agcgtctata tcatttttat gcttgcattt taaatcacaa cagtagagtt tgttatacag
     5281 taactatcgc acaaaaaata aagggaagga ccccatgtca tttgattcat tacaaaagct
     5341 ttttgaattt gatgctctgt tgcatatggt gattgaaccc gccagccgtt ttgagcctgt
     5401 caatagccgg cttgttgatt gtatcgaggt ttataaacca cgcgtaatag tgaagattgg
     5461 cctgggcaac cctgcatata ttgaaagcat aatggagcat tcaaaggcat tagttgttgt
     5521 ggttgaacct tctgtaaccg tgctacagtc gttttataat gcatatggga ataaacagtg
     5581 gatgaaccgc ctgtatacta tagcggggaa tttcagccag ttccctgttg attattataa
     5641 agcagatatg attatatgca ttgattgtct ggatttactt gatacgggca gggcaattga
     5701 tgaattcaga cgttcacttc aatttgaagg ggtattcttt tatgccgggt ttgtacttcc
     5761 taatgaagat gctgaaggaa tctttgatga gatagctcat gttatcaatc cgctacatac
     5821 ggattattac cttgaagatg atttaaaaac atttatgaca ctcaatgaat ttggtattgt
     5881 gaagctaaac acgcagaaga ttaatatgaa tatattattg cttgctgatt acctgcaaac
     5941 aagaagcagt gaattagaaa agctcattga agaacataaa gacacactta ctgctcttta
     6001 tgatttgcac agcgatggaa cggtagtgtt accttacatg atcgcagctt ttatacgaca
     6061 gaaaccttct caagaagaag ctctatagta taatgggtca tatccttctt tgccattgta
     6121 tttttcactt tgttgtttca tatagcctaa atatgccatt tcgttaactt ctaatccaca
     6181 ggttttgagt gctttcacga catcgtcaaa gttgggcgga caccctttca cttccaccga
     6241 ttctttgatg gtgggattat tcttgttggc ttttatgatg caattgccca acaacactgt
     6301 tttatcataa ccaggacgcg cctgcatctt tttgccattt aaaatttcta cttttggaag
     6361 aggctgccct ttaaacgcag acatcaccag aatattgcac atattggcaa ttggtgagca
     6421 gcccgagcat agcgtgtcat cgtattttgg caacgcaacc ccttgaatac ccattttagc
     6481 aaatgcgcca gggccggtgt tatccgctgt ccatgaccaa tcccacttta atggctggat
     6541 gtgatcttcc agtttttctc ctttcactgc ataatcgctc agtaccagcg atttgttatg
     6601 tcgtgtcgca taggttacaa aatgagctat atcagatggt tcatagccca tgacctttgc
     6661 accaataata tcggcagcta aaatatcagt agaagcaatg ataatatctt ttttgtatgc
     6721 gttgccaaaa tggagggctc ctttctccag tgcatatatt ccatcaataa tggtaagttc
     6781 aggttttaca tagtcagcaa caaacgggaa acagtattct aaatttagct gtgggtgatg
     6841 acaaagtttt tttgatgatg ccttaagaca tcctttcaaa tttttcagcc ccagtgaaat
     6901 tttagtttgt ccatgagttt ttaatacagg gaagttgata aaaaaatcac actccaatgc
     6961 ttcttttgct atcattagat gagtggatgc atcgatggag catttttctg attttgattc
     7021 attaaaatca acaagcttta ctccataatg tgatgctaaa tcagtatatc ccaatcctgc
     7081 aaaggctgcc attgttccaa cacctttttt aacctcaacc gaaccttcgc caatggtaat
     7141 atcgttacat ccatattctt ttaagattat tataaggtct tcaaccatcc ttgttgtagt
     7201 aaatacacca tacggagcag gagggaataa ttcatcccat gccaccaggt tgggtttaat
     7261 taacacttta tcaccgggtt ttaacttgct aaaaccatca cataattcta tagcctgctt
     7321 taaagaatca gtacttttgg aatattttac caatgataca ctttgcatga tagcctccat
     7381 ttgattaaaa gagctactta ctataagaaa taatcatgat aaatatgcaa tactttttta
     7441 aaaatcgcaa aagtgaatat acaattccat agcttataaa aatgcttaac gattttaagt
     7501 tgatgggcag caactgcagc aaatgaagca ccaaatgcgt atgaccatac aatatctacc
     7561 tgtaccatag taaaaacctc cttatggtaa aatatgttga tttgttttag gtaaagcact
     7621 ttgtttttgt ttacaaaatg cttctatttg agctaaatat tttgagtttt tacccctatc
     7681 tatatattca ggctttttta gcatttcttc aaatttgatg atggtatagt atggatagct
     7741 gtcgattaaa tggtttacgg caaaaagtgg aacgccggta ggatctgcat gaataatata
     7801 aaagcatttt accttttccc tttgcaatat gtgagctatc atatatgctg aaacttcata
     7861 catcctgaca aatccttcaa cttgtgggac atattgttcg ctttcaggtg gaagtaaccc
     7921 atagcataat acttcaaatc ttccatagtc ccaatctttt gtaacctgtg tccgtaccac
     7981 cccatccctt ggctttaaag gccatggcat ggccgtggcg ttgtgtatta tcatatcttc
     8041 atcattgaat atttttattg tttctgctat gaagcactta tacataaatt ctttataggc
     8101 aggtacatca cgcacaagct gttcaactac ctcaggcttt acatacatga ttttttctgc
     8161 ttttacctgc acaataccag atggcaactt ccttgtgtac aataaaacat catctttctt
     8221 ttttaataat ttccaatcag gggcatattc aatccatttg ctttttatat cattactggc
     8281 acattcagtc caatcctcat gtaggcaggt tatttgattt tggctggata cagccgaatc
     8341 atagacggga atacatacaa ctattacaaa aagtggtata taccatttta agcatgtatg
     8401 cattgcaaac ccccataata caaaattgct attataatac atgataagaa atctcttgtc
     8461 atgtaaaaag tagaggttgc taaatttgtc caatcattaa agtatattat ttcctatagt
     8521 tgcttggttt tgattgtgta aattttttaa atgttctgtt aaatgtcttt agattttcaa
     8581 atcccactgc catggcaata cactatactc atccgggcaa tctatttttt gtactgcact
     8641 ttcatcaatg tcatagttgt cgcaacccga taatgcaatg ctgccggcag taagagttac
     8701 aataactaaa ataacataca gtgttttaaa tgatgcggta atgccgttca ttttcatggt
     8761 atgcagcagt acaggtattg atgaaaagca atatagtatt attttatata agctgtcaat
     8821 tgctattata tcttaatact aaaaatactg gtataacaaa agtaaatgct ttcaatagca
     8881 gtcgtcatct ccccatccat tgggggggat gatggaccat caaaaatttc cacggtagtt
     8941 ataataacta tatcttcttt attacaaagt aataatcttt attgacatta attatcagta
     9001 ctcttacaat cattggcata ttgctggagg atacacaatg aaagcacgaa taagcatctc
     9061 cctttttctt cttggtttag gcgggcagat agcatgggct attgaaaatc aattttttaa
     9121 taccttttta tatgacagaa taatccctga ccctaagtat gtttccatca tggtggcgct
     9181 ttctgcagtc acagcaacgg ttactgccat tatcatgggg gcgttctctg attcaattgg
     9241 caaacgcaag ccatttcttc tgtatggata tttagcatgg agtgtctcta tatgggtaat
     9301 accaatggcg gaatggatca agccggtaat cgtcgctgca tggatggtca tcatccttga
     9361 ttcagttatg acattttttg gttcaacatc gtatgatgca gtatacaatg cctatttaac
     9421 cgatgtaact accgatgcca accgcgggaa agctcaaggg ttactatcgc ttgcaacatg
     9481 gattgcgctt ttacttgttt atggcgcatc agggcaaatt gtagaatcaa tgggatactt
     9541 tttcttcttt ggtgcagccg gtgcgctggt attactatca ggtgttatag gcggcattgc
     9601 agcagtggag ccacaaaggg cgatagttac cgatagtaag tctatcgtaa aaagaataag
     9661 gggaagctta aatatgcaag cactgcggca caataaaaat cttttgtatg tgttgcttgc
     9721 tataggattc tgggggatgg cattcaatgt ttttttccca tacctattga tttacctgaa
     9781 acattttttg aaaattgata ttgctacgtc atcggttatt atagcagtat ctatctttat
     9841 tggcggaata gcagcttcca tccctgccgg cattattgct gataaaatag ggcgtaaacc
     9901 tgttgctatt tttgctgtga tattaatttc attttcgctt attgcatttt cgtatgctcg
     9961 tttaccgata gctatcggca tattagcaac cttatggata attgctcaga caatatggac
    10021 agttgctaca ggagcatgga gtaaagattt attccctgaa gagaggcgag gtgagtttgc
    10081 aggatatttt acattgttct atgttgcatt tacaatgata cctggtccgc tcattggggc
    10141 gatagttgct gacagatggg gtatacgggc aaccattgat ggcaaaccag gtattattcc
    10201 aacacctgag atatttgttg tatctgctgt gctcatttta attactatta tcccgctgtt
    10261 atttgcacaa gaacaatagt agtaattgtg ctattgggta tttttgatat atttttcaat
    10321 agcatcaatg tcatcttgtg acagagcgca cggtgctttg taatctgcat tgatttgttt
    10381 tgctgctttg ccaacaacct tccagaatat tccaaaatta tcaatatttg ctctggcaac
    10441 aaacataggt cctctgaatc ctttcatgat gcccggcatc tgcatatatc cccacacccc
    10501 cgaagcaaat ttataccatt gaacagtggt tggtgcaaaa gcccactcta cagttttata
    10561 ctcttcaact aacgcaccac cgtatgtgca tattgccctg ccatccttat tttcaatggt
    10621 aagttgaact tttggcattg agtcacaatc tcttcgtgac atggcgaaaa cctgtttgtt
    10681 cctcacctga ccggtgtatt tattttttgt caaaatccag tcattgccac caactgcttt
    10741 tgtgcccaat ttttgggtca gttcacttct gttccattca tcacagacaa attttgtaaa
    10801 ttcaggactt aaaaaaacaa tagaattgct ctgagcatgg gtcaatgaaa cattgaagta
    10861 aagagcaatt gtaaaaaggg taacaactga taaaatgaaa atgaattttt tcatgggtat
    10921 cacctcgtat taaaaattgt attggtatga attataagat atttagttat taaaatgtca
    10981 ataaaattat taaataataa taaactatat atctaaaata ttaaaaagaa ataattgtat
    11041 gtacgatagt tactatctat tataatacag tattaatttt tatatttgat ttgacatgat
    11101 tggttgcata cgattacata ttcaaaatga ggcagacagc acgacatggt gcaatgcatc
    11161 cagaaggcaa cacaatgtaa tttttttaaa aagtcatcct gaacaaagtg aagaatctcg
    11221 ctgatttgtg aacgaataga ttcttcggga atgcgtccct tagaatgaca atgtcgagaa
    11281 cgtcatcctg aacaatgtcc ttaactggct tcaggatcta acgttacaca tctggattcc
    11341 ggttcacgcc cggaatgcca ttgaagggat aatcatattt tagggaggct gtcatgaaac
    11401 ggtttatcat gtacagtatt gctggtatta ttcttgtgat tgcagtaagt atatcatatc
    11461 tgataataac agagtatata ttaattcgtt ttcctgtgct ggtacaaaca aatcctaaaa
    11521 tcacatttat aataggagat tcatattttc gtgaagatga aaaggcgcag tggaagatag
    11581 cggttgttgg tcaaacattg tatcaaggtt actgggtaaa gactgacccc gattcaagaa
    11641 tggatattcg tttccatgat aacaccgcaa tacagctatc agaaagttca gagatggcta
    11701 tcgattcatt aacagtaaaa aaattaggat tacagataca tacaggatcg atgtatggga
    11761 agtttgataa gttgtttaaa gaacatgatc tccatgtaaa aactccaacg gcatttgctg
    11821 caatacgggg aactgattta ggttttatgg ttgttccaga atcttatgga aatgatagtc
    11881 ttatgggaca acaaaaaaaa tctgcatata acaagggcaa gaataaagga caaacattaa
    11941 aaacaaaagg tgcgactcaa aatcctgatt caggtgaagc agacttgcaa acgctggttt
    12001 attctctttc ggggattacg gagatataca atccaatatt tgatgatcag aaggttttgc
    12061 tgtcatatcg caataaagtc atcataggtg aagagagcac accaacaaat ccagaagagt
    12121 tgagtgagga tgaaatcaaa acgttacagg aaaaactaaa ctcaatccat tttgatgaag
    12181 tattgttaat tactgataaa ataacattta attttggtag tgcagaaata ctgccttcat
    12241 cgtatcctga attggataaa atagtggaaa tcttaaaaca gaaaaaggtt cgcgtaagga
    12301 ttgaaggaca taccgatgac cagggtgatg cagcatttaa tcagcaactt tcagaaaaaa
    12361 gagctgaggc aataaaaaat tatcttgtta cgaaagggct tgatcctgag cgttttgaaa
    12421 cagtggggtt tggtgaatca aaaccaatag ctgacaataa aaatgaggtt ggcaggtcac
    12481 aaaatagacg agttgagttc cttatcatag aaaaataaaa aaaccgtgct caatttttat
    12541 ttgacaacaa tacaccttcc aatagtgttg ctatcggaag gttgtccgag tggtctatgg
    12601 aggcggtctt gaaaaccgtt gggtgaaagc cccgggggtt cgaatccctc accttccgaa
    12661 taacggagag atgcccgaga ggccgaaggg gacggtttgc taaaccgttg tactggaaac
    12721 ggtaccgagg gttcgaatcc ctctctctcc gaatggggaa attacactat tacagcttac
    12781 agatagccaa gtacagtaaa aaactctacg cacaatttat gcactattat gctgtggtta
    12841 agcaattttg ctttgaacac aaacagcaga ttattgcatc tattgcgtat ataccatata
    12901 tcggatggtt gtatccctta tatgcctgtg aaggagatgg agtaatccag aaacatgcaa
    12961 agcgcggatt attatacgca ggtgtatttg tttttgctgc tctggtttta tttttgttgt
    13021 catttatact gccacgatca tggcgcctgc tcagttttat agttaccatt gttttgtata
    13081 tcaattattt tatttactat gcgatagtta ttattcatat attcaatatc tggaaaggga
    13141 aagatttttc tataccaaag tttgaaattt atcttgaaaa aattgagcag tatatataaa
    13201 tattattgta ttgtttttat acatgcgtcc atagctcagt tggatagagc gtcggactac
    13261 gaatccgaag gccagaggtt caaatcctct tggacgcaca taagcgatgt ttcattacac
    13321 cttaattttt atgccaaata tatacgttgt taaatcttca gtatcaactg ctttttcagt
    13381 caattgtaat aacccgctca atgtaagtca ccatagttaa tttttaaaat ttcaggtggg
    13441 ggcccccaat aaaattggca gcagtgtata cctttaacct taaaaaaatc aaaagtatac
    13501 gaacgatttt cgtaatatga tagatagtta tcggttaatg attcttttct gcgatgaatt
    13561 acgtagttaa aaaattcatc cagcacagta tccacacaaa attcaatagc tttggcaatg
    13621 gaaaccttcc agattttttt aatatcaagt aaaaattcat attcactcca gctgatacat
    13681 agatgaaaac gaatccattt gatatctgta tttctatcac gataggagat gtttttaaat
    13741 acctgtgttt tccttttttc tttttttgca gcatactgaa tgaggtaact aataagtttg
    13801 ctgacggata catctaatat ttttgcataa tgtttgagta ctataatatg gttacttgat
    13861 atgcaggtag ttgtttcaaa attcataaaa cccctccaaa gttgtatgta gaaatttgct
    13921 gatactttta agattatttt tatgtgatta atgattaaaa acttttactg ctattttgct
    13981 attacaaatg cattgtaaaa ttttctacac agtctcttga tataattcaa aagactgtgt
    14041 aattaaaatt tatgtaaagt attaataaat taacttttta tgatacggca tattctgcaa
    14101 ccttgtcacc aacctgagtt gtagttaagc ccattttgcc ggcagcaagt gacttcatat
    14161 cccgttgcaa tacatcaata acaccttttt caatacgtgc agcagtcttt tcttcgccta
    14221 aatgttccat catcatctgg gcggcgcata ttgctgccag agggttgata acattttttc
    14281 cagtatattt tggagcagat ccaccaattg gctcaaacat ggaaacacct tcaggattga
    14341 tattacctcc cgcagcaatc cccatgcctc cctgtgtcat ggcgcctaaa tcggttataa
    14401 tatcgccaaa catattcccg gtaacaagaa catcaaacca ttcaggattt ttcaccatcc
    14461 acatgcaggt ggcatcaaca tggtagtaat cgcgtttaat atctggatat tctttctgtc
    14521 caatttcatg aaatgctctt tcccacagat catacacata ggttaataca tttgtcttgc
    14581 caacaagtgc taatgttttt cttttattac gtttgcgggc atattcaaat gcataccgca
    14641 aacatcgttc aacctggaag cgattgtaaa tcataatctg cattgcttct tcatgcggcg
    14701 ttcccttcat ggtaactccg cctataccag tatacacgtc gcctgagttt tcgcgtacca
    14761 caatatagtc aatatgttca ggacctttat ctttcaatgg tgtttcaaca tttggataaa
    14821 gcttgattgg acgtagattt atgtactggt ctaattcaaa acgaagtgcc agtaaaatac
    14881 ccttttctaa tatacctggt tttacatcgg gatgacctat tgctcctaaa aaaatggcat
    14941 catgtttttt taattcgtcg atagctcctt caggtaacgt ctcacctgtt ttaagataac
    15001 gctcgccgcc aaaatcataa ttggtaaaat ggagagatat
//
LOCUS       DAOSOX010000037        14965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4018_length_14965_cov_8.768142, whole genome shotgun sequence.
ACCESSION   DAOSOX010000037 DAOSOX010000000
VERSION     DAOSOX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_14965_cov_8.768142"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="PLX22_04360"
     CDS             <1..2040
                     /locus_tag="PLX22_04360"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT19162.1"
                     /translation="YSYFGFCAFGILFTMFLTYAFPDKLDLTLINKITQASTVLTFSA
                     LFVLSLIFPKTEKQVPFFIIVLILIPAYIFAYIAVFTDLNITAAYFKDGELVREFKPF
                     YTIYSVVSFVYILLGVINFIRKYITTKIDLYRKQMRFVFIGTGIAMTFAAVFSIIMPR
                     FFNFVDLYVLGPSLAAFIGVSSLFYAVISYQLMDIRTAIHKTTMYAIISAVIFLPIFA
                     LLQIYDINIYGLKNIPVFVIALVIVVVFMILSKYVQPVIDKAFKRKHYALQEYLDTFV
                     REISNLRDIDELLKRTVSTIHNTFFLKTTYILMYDNESRSYQINYSMGMAPVSPSIPR
                     LSPIVRWFNRNQTIVTIDNVYMDEASFKDVRDEFIQYFTVNNIVIMIPIYHRRVLVGA
                     LCLGEKDTFASYKPDEIELLKFLSSETNINISHAITFQEAKKEQLLQRTISVSSDLLS
                     KAVPKALPNITGIKFGAFYIPRQGGGIDYFDFIRPGLQGIGTIATDIAGIGIQSALYS
                     VLMRSAFHASLSDAHSSYNIIQTLNKVIYNYSQGKGILITAYYYFIDARNMRLIYTNA
                     GFPPMELYRVEKSAFSSLDTEGIPLGYDPTFNYGMGRTNILRGDIGILYSKALITSKN
                     SRGEVFTLERLQSIIKDNRMKMPSDIATIIQDEFKAFLGISSPESDIVVILFKAY"
     gene            complement(2041..2376)
                     /locus_tag="PLX22_04365"
     CDS             complement(2041..2376)
                     /locus_tag="PLX22_04365"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT19163.1"
                     /translation="MCKDITVYGDGTQYRDFIFVKDVVQVNMLALEKLDKGFHYYNVG
                     YGKATDINSLITILQNIYKKTIKVHYVEARPGDIKGSISDPAKVIRELGFSPQYSVEE
                     GLTLMAGVM"
     gene            complement(2369..2974)
                     /locus_tag="PLX22_04370"
     CDS             complement(2369..2974)
                     /locus_tag="PLX22_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOT19164.1"
                     /translation="MKICITGEAGFIGSHIVEECIKNHYDVIVFDNFSTGKEENLSFI
                     KQYPTARIKVIHGDVADWYALHEAVKGCDAVFHEAAVASVQQSIENPPATFESNAKGT
                     LHVLEACRQNGVKKVIFACSAAIFGDHPEIPKKENSPVFPKSPYGADKYISEVYLRLY
                     NELYGLTTVSLRYFNVFGPGQDPSSPYSGVISIFTNKDGYV"
     gene            3581..5023
                     /gene="purF"
                     /locus_tag="PLX22_04375"
     CDS             3581..5023
                     /gene="purF"
                     /locus_tag="PLX22_04375"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT19165.1"
                     /translation="MKTEYEYCDCKPREECGVFGIYNNPRAANLTYLGLYNLQHRGQE
                     SSGIASSDGEHIYRYAGMGKVVDVFTKEHINNLKGTIAIGHNRYSTTGSSFLRNAQPL
                     RVHSVLGPIVLAHNGNLVNYAILRRALEKEGAIFQTSIDSEVIVHLMARSGLTNFLDA
                     LIYALKAIKGAYSLLVMNNNTIYAIRDPYGFRPLVLGKLGNSMVVASETCAFDIIEAE
                     YIREIEPGEMVEISPNGMKSYFPFGSVKNSLCVFEYIYFSRPDSIIFGQSVHEMRLRM
                     GAVLAKQSPVKADIVVPIPDSSVCAALGYSRASGIPYEMGMIRSHYIGRTFIEPEQRI
                     RDFGVKIKFNIVRSAIEGKRIVVVDDSIMRGTTMRKIIKMFRNGGAKEIHVRISAPPT
                     RFPCFYGIDIPTRSELIASSHAIDEIQKYLRVDSLAYMTVETMLASLGVHHGKFCTAC
                     FDGNYPIEFQESEDYQKCLFDDIVACGEYY"
     gene            5070..5142
                     /locus_tag="PLX22_04380"
     tRNA            5070..5142
                     /locus_tag="PLX22_04380"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5103..5105,aa:Trp,seq:cca)
     gene            5158..5877
                     /locus_tag="PLX22_04385"
     CDS             5158..5877
                     /locus_tag="PLX22_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19166.1"
                     /translation="MTQTKIITFDTIAFPVDNSLLKLLHQKTDCQELSHSLHKGSDAY
                     INTINTFSGDSLSNNQLLSALPDSIYQLYVQAYHIAKPEAAIGKANAINSTIAIPDAI
                     VTSDRSYAFIFTLGIRYENWYISLPGLTHQYHASIIGMWLCNQYVQELLKKLFGTGGY
                     VWYPGSSKELPVTLNHAIYAMLKTQAQNIGIALNRHCMFQPLHTVAGFALPYGNSHSV
                     CSSCNLQQCQFRNILTELDNC"
     gene            6003..7151
                     /gene="alr"
                     /locus_tag="PLX22_04390"
     CDS             6003..7151
                     /gene="alr"
                     /locus_tag="PLX22_04390"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT19167.1"
                     /translation="MILTNPAYCNRPTRAEISIPNLLNNFAIVRSLISPQVKVMAMVK
                     ANAYGHGIVRISQELIKAGVNYLGVAYLEEALFLRQHGIHTPILVCGAINSEQIPEFI
                     INDVAITSSSIDKSKAIQTAAKALGKKALVHLKIDTGMERIGVHWYNAQKFIEETLQL
                     PDIQVVGIFSHLAKAESDSHFTQEQITRFESIVNYMDRQSILPPYVHLANSAAIINNP
                     ESHFTMVRPGIMLYGYNPNGYNPSIEFNRKKLLPVMTLKTKVSYFKVVPANTGISYNH
                     LYKTTTQTRVVTLPVGYGDGYSRLLSNQGEVIIRGKRYPVVGAICMDQLMVDIGYDGT
                     AYNGDDVLLFGQMDGYSIPLESLCEKIGTITYEVLTNISPRVPRIYIQ"
     gene            7691..8503
                     /locus_tag="PLX22_04395"
     CDS             7691..8503
                     /locus_tag="PLX22_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOT19168.1"
                     /translation="MIKDAIYYDSYKEDRDLILEYNQTSLADVNLVNILGIGIDNCTR
                     QQAVVKVLRMIEEGGVHHVMALNPYKLHRLITNNDLNLIASKADLKIAVGAGLPWAAS
                     FLGKPLKERIHFVSFLMDIIRIAEIKEITIFMVGAKPEIIEQAYANIKKSFPMIRIVG
                     RHGGYFNKERASNVIEAMRKSEAQIILVGLGFPKEDKWIYQIKNQFKNTVFIGIGGSI
                     DIISGQKHKAPAFFMEKGLDWFYRIITRPWRYLRLLRLLLFYIQVVWQKIRQ"
     gene            complement(8504..8719)
                     /locus_tag="PLX22_04400"
     CDS             complement(8504..8719)
                     /locus_tag="PLX22_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19169.1"
                     /translation="MEYVSVILCRNCGSRYVEVNEWTQDKKAVFHCRTCGKKEIVEWF
                     TLGRCQVTPSELQKTRDTKAKPGKYER"
     gene            complement(8736..9998)
                     /locus_tag="PLX22_04405"
     CDS             complement(8736..9998)
                     /locus_tag="PLX22_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT19170.1"
                     /translation="MTDSNNEQKYNVAYLERQLFNLSKLVEINRIINSTLDMAKLFTI
                     IMEIIKEIMETEASTLFLFDEKTNELVFKVALGEAGDKLQEKYRVAMGQGIAGWVAKE
                     RKGVYVNDVYLDERFDPNFDKQTGFTTNSIICVPLLYKGKLLGVIQAINPINRPDFTD
                     EDMNLFRAFADQAVLAVQNAIFFERAIEEARMRNELSAAHEIQHSLHPSIANDYVFCE
                     IAARYLPAREVGGEFYEIKPYDDAVAIAVCDVHDKGVPGALNAALVNGLLKGLMNVYG
                     RVVAPIFLNTLQVVASHHINNVSLFLGMIDNKTKTLTFMNTGEAYPVIIRDSIAHYLR
                     FSHSLYNNKKIVVKLKHEDMVVIVTDGIVNCKNRNARLFGLKQVMDTCSQFSTPESCI
                     AGLLDTVKNFMQGLTQREDISVIAVKVK"
     gene            10221..10718
                     /locus_tag="PLX22_04410"
     CDS             10221..10718
                     /locus_tag="PLX22_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT19171.1"
                     /translation="MTYLIDGYNLIYKFPHLEELMLQDNLIEARRELLDLLKMFAKLT
                     RKRIRIVFDGQKTSDIPISSEKVYTIDVYYSLHYSADFLIKEFIRKDLQPRNTTVVTS
                     DKDIIEYVSRFKTKTMKSEDFALFVNQTIEDFISAQTPEKEENPNLSQKEIEYWERLF
                     TSKKK"
     gene            complement(10719..11486)
                     /locus_tag="PLX22_04415"
     CDS             complement(10719..11486)
                     /locus_tag="PLX22_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT19172.1"
                     /translation="MLTLGMDIGSITAKAVVLNGGTIVGSKVIFTGFNAAKAGEKVVQ
                     ELLAELGLTLEQIERIVATGYGRKSVGFAHATVTEITCHARGAHFLDNSVRSIIDIGG
                     QDSKVIVIDETGRVKDFAMNDKCAAGTGRFLEVMARALEVDLDVFGELSLKANNPSKI
                     SSLCTVFAESEVISLIAKGESRDNIIAGIHQSIASRVMAMASRVGIASPVMMTGGVAK
                     NIGVVNAIEKLLNSPVKVSKDAQVIGALGAAILAAEN"
     gene            complement(11542..12057)
                     /locus_tag="PLX22_04420"
     CDS             complement(11542..12057)
                     /locus_tag="PLX22_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19173.1"
                     /translation="MKNFRAIVPFIIVLVALCCSKHGVRSGAMLCTMHNAHNFYSKAD
                     FARCMGIDENALEDYFIFSQSAKAFSDTASTESMLKSTVCDTIVDEVMLRIQTMFTNK
                     QFARWHFTEMSLPVQFRTFIQQGSIPEMMVVGLVKKEDLSARAIIYYLPLEYKMDIIG
                     REKENGKMPDW"
     gene            complement(12066..12935)
                     /locus_tag="PLX22_04425"
     CDS             complement(12066..12935)
                     /locus_tag="PLX22_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOT19174.1"
                     /translation="MKYKKPGTIVLIAITVIFIHATLVSDTRHGTWVIYNVPASPQSE
                     KTIPVKIYFPKSYSDSSRVIIAFHEYDGSMNSWQNNTSIAYYANQYNMVIVCPHMPRS
                     VYESYYFDQTTVKWNEMPALLWIGQLLLPYLNNTVGLNITKQKLGVCGFSIGARGALR
                     VGEVYHDFVGAIACLSGYYDMLSHTKNHMFTAVYGKYNDFQQRWAQTDNAIDEAKNLG
                     DVAVFIAHGNKDSRVPMEQAFMLALKLKQLQKEYKSGYNFTFVEKKYKMHDWNYCQSV
                     LPDMMAFFNENLK"
     gene            complement(13172..13846)
                     /gene="radC"
                     /locus_tag="PLX22_04430"
     CDS             complement(13172..13846)
                     /gene="radC"
                     /locus_tag="PLX22_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT19175.1"
                     /translation="MESKNDLPIQRCIKGEPLEALSDVELLAVLIGSGVKGKNVMECA
                     FYVIEQFGGMNGIYHAGIRELAHTDGIGFVKAIRIKAAFELGRRLIVPKNYEGSIDSP
                     RMVWEFIVGQIACNKQEEFWALILDNKNRLLKKTRVSIGTISESIVHPREVFRDAIRE
                     AASSIIIAHNHPSGLLKPSVNDIEITRRVAQVGAIVGIQLLDHVIVTDNDYLSLRETD
                     EKLFLA"
     gene            14212..14772
                     /locus_tag="PLX22_04435"
     CDS             14212..14772
                     /locus_tag="PLX22_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19176.1"
                     /translation="MKVSIDAILQSAKQLKNTNSVDEQKSGNKHKVSNDKVSLEHKVL
                     ARLDDLHNQIKDIQTTLSRYQVIDEGIRLIIEDNKNGGNNKQSIINSTLYNNSPVLKE
                     YLGDSSTNEYIVHKQEQVKNLITDSISHLNRLQVEFENIMASDVAHTPYIKDKVDAID
                     ALIPRNVKNPSQLHHVHSDSVLRLIQ"
BASE COUNT         4639 a         3087 c         2555 g         4684 t
ORIGIN      
        1 tactcgtatt ttggtttttg tgcctttggt atccttttta ccatgttttt aacctacgca
       61 tttcctgata aacttgatct tacccttatt aataagataa cgcaggcttc cactgtacta
      121 acattctccg cactatttgt attatcatta atatttccaa aaacagaaaa acaggtccct
      181 ttcttcatta ttgtattaat cttaatccct gcttatatct ttgcttatat agcagtattc
      241 accgatttaa atatcaccgc tgcatatttc aaggatgggg aattggtgcg tgaatttaag
      301 ccattttata ccatatattc agttgtttct tttgtttata tactattagg ggttataaac
      361 ttcatacgaa agtacataac tacaaaaata gatctctacc gtaagcagat gaggtttgta
      421 tttattggta caggtattgc catgacattt gctgcagtat tttcgattat catgccacgt
      481 ttctttaatt ttgttgacct ctatgtttta gggccatcgc ttgcagcatt cattggagta
      541 tcatctctgt tctatgcagt catttcatat caactgatgg atatacgaac cgccatacac
      601 aaaaccacca tgtacgccat catttccgcg gtcatattcc ttcccatatt tgcactgcta
      661 cagatctatg acattaacat ctatgggttg aaaaatattc ctgtatttgt gatagctctg
      721 gttattgttg ttgtttttat gatcctttca aaatatgttc agcccgtcat tgataaagct
      781 tttaagcgca agcactatgc attacaggaa taccttgata ccttcgtcag ggaaattagc
      841 aatctacgag atattgatga attattaaaa aggactgttt caaccatcca caacactttc
      901 ttcttaaaaa caacctatat tctgatgtat gacaatgaat cacgcagcta tcaaataaac
      961 tactctatgg gcatggcgcc ggtatctccc tccataccgc ggttatcgcc aattgtacgc
     1021 tggtttaacc gtaatcaaac gatagttact atagataatg tgtacatgga tgaagcatca
     1081 ttcaaagatg ttcgtgatga attcatccaa tattttacag taaataatat tgttatcatg
     1141 atacccatat atcatcgaag ggttttagta ggggcactat gcttaggtga aaaagatacg
     1201 tttgcatcat acaaacctga tgagatagaa cttttaaaat ttctttccag cgaaacaaat
     1261 atcaatattt ctcatgcgat aacatttcag gaagctaaaa aagaacaatt gttgcaaaga
     1321 acaatctccg tttcaagcga tctgctatca aaagctgtac caaaagcatt gccaaacatt
     1381 accggaatca aatttggtgc attctatata ccgcgccagg gcggtggtat tgattacttt
     1441 gacttcatac gacctggatt acagggtata ggaactatcg ccaccgacat tgccggaata
     1501 ggtattcaaa gtgcattgta ttcggtgctc atgcgttcag cattccatgc aagtctaagc
     1561 gacgcacatt cttcatacaa tatcatccag acattaaata aggtaattta taattattcg
     1621 caagggaagg gaattttgat tactgcttat tactacttta ttgatgcccg caatatgcgt
     1681 cttatttata ccaacgcagg tttcccgcca atggaactgt accgcgtcga aaaaagtgct
     1741 ttttcatcac tggacactga agggatacca ttgggatatg atccaacgtt taactacggt
     1801 atgggcagaa caaatatcct gcgaggcgat atcggaattt tatattcaaa agcattgatt
     1861 acatcaaaaa attcacgtgg cgaggtcttc acgctggaac gtctgcaatc tattataaaa
     1921 gataaccgaa tgaaaatgcc atcagatatt gcaaccataa tacaggatga gttcaaagca
     1981 tttttaggta taagctcccc tgaatctgat attgttgtga tactatttaa agcatattaa
     2041 ttacatgaca ccggccatga gtgttaatcc ttcctcaaca ctatactgtg gagaaaatcc
     2101 taactccctg attacctttg ccgggtcact tattgatcct ttgatatcac cgggtcttgc
     2161 ttcaacataa tgtactttaa tagttttttt ataaatattt tgcaagatag ttatcagaga
     2221 atttatgtca gtggctttac catatcccac attgtaataa tgaaaacctt tgtccagttt
     2281 ttccagagcc agcatattta cctgtacaac atctttcaca aatataaaat cacggtattg
     2341 cgttccatca ccatatacag tgatatcctt acacatagcc atccttattt gtaaaaattg
     2401 aaataacacc cgagtatggt gacgaaggat cctgccctgg accaaacaca ttgaaataac
     2461 ggagcgatac cgttgttaat ccatacaact cattatacag tcgaagataa acctccgaaa
     2521 tatatttatc tgcaccatac ggtgattttg ggaaaaccgg agaattttct ttcttgggaa
     2581 tttctggatg atcaccaaat atagccgctg aacaggcaaa tattactttt ttaaccccat
     2641 tttgtctaca tgcttcaaga acatgtaacg ttccctttgc attcgattca aatgttgccg
     2701 gaggattttc tattgattgc tgtaccgaag caacagcagc ttcatgaaac acagcatcac
     2761 agcctttaac agcttcatgc aacgcatacc aatcagcaac atcaccgtgt ataactttta
     2821 tcctggcagt gggatattgt ttaataaatg ataaattttc ctctttacct gttgaaaaat
     2881 tatcaaagac tatcacatca tagtggtttt ttatacattc ttcaactata tgtgagccaa
     2941 taaatcctgc ctcaccggta atacatattt tcatggtgat agctccctgg taagtttcct
     3001 acaaaaatag cattagtaac ctcagccctg cccatctcct tcgcaaggcg agggaaattg
     3061 acacagcgtt gtcattctga ggaagcggta gcaactgaag aatctctcct ttcaaaaaac
     3121 gacagtattc ttcactccgt tcagaatgac tccccacgtt tcaggatgac cgtcgccagc
     3181 ttattttcat tccatctgga taattataat accgccacgc acaactctct gttattgtcc
     3241 gtttgtcaat cattagcctt agattgtcta tcaatgcaat gaactattca ttagtaacta
     3301 tcgccgatgg tactgacact gtattatgat ttcaagcaga ttattgtaat ttttaaaata
     3361 gttaatttta ccatacatct gattatccac gtaccatagc aatactaact ttttaattta
     3421 attttttatt gattttatat aaaagccaat tatatttgta attgtgtatg aattaatagt
     3481 gacaatcagg ttattatttc tttttaggta cagtctattg tcagggtgtg ttataaccat
     3541 ttctcatcat aacttaatct aaccattttt caggaaaaat atgaaaacag aatatgagta
     3601 ttgtgattgt aagcctcgtg aagaatgtgg tgttttcggt atttacaaca accccagagc
     3661 agcaaatctt acctaccttg gactttacaa tctccagcat cgcgggcagg aatcaagcgg
     3721 tattgcctca tcagatggcg agcatatcta tcgctatgcc ggcatgggta aggttgtaga
     3781 tgtatttacc aaagagcata tcaataatct caaaggtact atagctatag ggcataaccg
     3841 ctactctacc actggctcat cgtttttacg caatgctcag ccacttcgtg ttcattcagt
     3901 gctgggaccg atagttcttg ctcataatgg caaccttgta aactatgcca ttctgcgaag
     3961 agctcttgag aaggaaggag ccatcttcca gacatccatc gatagcgagg taatagtcca
     4021 tttgatggct cgatcaggac ttaccaattt tctggatgcc cttatttatg ctctgaaggc
     4081 tattaaaggt gcttactcgt tgcttgtcat gaacaataac accatatacg ctatacggga
     4141 cccttatggt ttcagaccgc ttgttcttgg taaattaggc aactccatgg tagttgcttc
     4201 agaaacctgt gcatttgata ttattgaggc tgaatatatt cgcgagattg aaccaggtga
     4261 aatggtggag atatcaccca atggtatgaa atcatatttc ccgtttggaa gtgttaaaaa
     4321 ttcattgtgc gtttttgaat atatctactt ttcacgaccc gacagcatta tatttggaca
     4381 atcggtgcat gagatgcgcc ttagaatggg agcagtgtta gcaaagcaat cgccggtaaa
     4441 agcagatata gtggttccca tccctgactc ttcagtttgt gcagcactgg gttactcgcg
     4501 tgccagcggc attccctatg aaatgggaat gatacgcagc cattatattg gccgaacatt
     4561 cattgaaccc gaacagcgta tacgcgactt tggagtaaaa attaaattta acattgttcg
     4621 ctcagcaata gaaggtaaac gaatcgttgt tgttgacgat tctataatgc gtggtacaac
     4681 catgcgcaag atcatcaaga tgtttagaaa tggcggcgct aaagaaattc atgtccgtat
     4741 ctcagcgcct cccactcgtt tcccctgttt ctatggtatt gacataccaa cacgttcaga
     4801 actaatagca tcctcacatg ccattgatga gattcaaaaa tatctgcgtg tggattcgct
     4861 tgcatatatg actgttgaaa caatgcttgc gtctcttggc gtccaccatg gcaaattttg
     4921 tactgcctgt tttgacggca actacccaat tgaatttcag gaatccgaag attatcaaaa
     4981 atgtcttttt gatgatattg tcgcctgtgg tgaatattat taaaaacgct tgacataacg
     5041 aatataattt ttctttgttc ttaatataca ggtgactagc tcaattggta gagcagcggt
     5101 ctccaaaacc gcaggttggg ggttcgagcc cctcgtcacc tgcattttta tatgtctatg
     5161 acacaaacta aaatcatcac ctttgacact atagcttttc ccgttgataa cagcctgctg
     5221 aagcttttgc atcagaaaac tgattgtcag gaactatcgc acagcttaca caaaggcagc
     5281 gatgcatata ttaataccat aaatactttt tctggcgata gtttgtccaa taatcaattg
     5341 ctctcagcat tgcctgattc aatatatcaa ctttatgtac aggcatatca catagcaaag
     5401 cctgaagctg ctattggtaa agcaaatgca atcaacagta ccattgctat acctgatgcg
     5461 atagttacct cagatagatc atatgccttc atctttacct tggggatacg atatgagaac
     5521 tggtacataa gcttacccgg actaacgcat caataccatg catcaataat cgggatgtgg
     5581 ctttgcaacc aatatgtgca ggaattatta aagaagctgt ttggaactgg cggctacgta
     5641 tggtatcctg gctcatcaaa agaactgcct gtaacactca accatgccat atatgcaatg
     5701 cttaaaacac aggcgcaaaa tataggtatt gcattaaatc gacattgcat gtttcaacca
     5761 ctgcacaccg tagcaggttt tgcattacct tatggtaaca gtcatagtgt ttgttcatca
     5821 tgcaatttac aacaatgcca gtttagaaat atcttgacag aattagataa ctgctaaaga
     5881 agtatagctt catcataatt cttttttaat atttcatgat ttaaattttt gatggttaaa
     5941 atgattttat ctgtatatgg caaaatttta aaattaaaac tatacgctat agattttaaa
     6001 ctatgatact aacaaatcct gcatattgca accgaccaac acgagctgaa atatcaatac
     6061 ccaatttatt gaataatttt gcaattgtac gctcgctcat ttcaccgcaa gtaaaagtca
     6121 tggcgatggt aaaagcaaac gcatatggac atggtattgt acgcatctct caggaactta
     6181 taaaagctgg tgttaactat ttaggtgttg cctatctgga agaggcgctg tttttgcgcc
     6241 agcatggcat acatacgcca atacttgttt gcggcgccat taactcagaa caaatccccg
     6301 aatttatcat aaacgatgtt gcaataacaa gctcttccat tgataaatca aaagctatac
     6361 aaactgcagc aaaagccctg ggcaaaaaag cgctggtaca cttaaagatt gacaccggta
     6421 tggaacgcat tggtgtccac tggtacaacg ctcaaaaatt tattgaggaa acactgcaac
     6481 tgccagatat tcaggttgtt ggtatttttt cacatttagc taaagctgaa tccgacagcc
     6541 atttcactca agaacaaatt acccgctttg aaagcattgt taactatatg gacagacagt
     6601 caattctgcc gccatacgtg catcttgcca actctgcagc aataataaat aatccagaat
     6661 ctcacttcac catggtacgt cctggcatca tgctgtatgg ctataatccc aatggctata
     6721 atccatcaat tgaatttaat aggaaaaagc ttttgccagt tatgacatta aaaactaaag
     6781 tttcatattt taaggttgtt cctgctaata ccggaataag ctataatcac ctttataaaa
     6841 ccacaacgca aacaagggtt gtaacattgc ctgttggata tggtgacggt tatagccgat
     6901 tgctttccaa tcaaggcgag gtgattattc gtggtaaaag gtatcctgtg gtaggcgcta
     6961 tatgcatgga tcaactcatg gtggatattg gttatgatgg aacggcgtat aatggggatg
     7021 atgttctgct gtttggtcaa atggatggat attccatccc actggaatct ctgtgtgaaa
     7081 aaattgggac tatcacctac gaagtgttaa caaacataag ccctcgagtg ccacgaatat
     7141 atattcaata gtttttgcat aattattcat ttattgtggc aaccaattaa gtaacagtga
     7201 taaaaaacca taatatacgg caggaatttg atatccccct gatatggatt accgcttaat
     7261 aacaattttt cggaaagaaa tatatacatg cttaaaatgc aacagaaaat ttcaggatta
     7321 ttcagaacgg ttagcgacac tgaacaattt tgtgttatgg taagtttcat ttcaacagtt
     7381 aaaaaacagt attttagtat tattgatgca ctcgaacgta ttcttaaggg tgacaggttt
     7441 agcttgaatt tatttccgag cagctgaata gttactattt ttataataat aattttttat
     7501 tgacataatc catactctat cgtttatctt ctctaatact caacacagtg gccattttat
     7561 ggtatttggc tttataacta ctatcccaca ccctatatac cgttttgttc taatagaaaa
     7621 gacattctat tagtcaaaga agtgagggca gggaaaacca caaatatata ttattaagag
     7681 aggatagtta atgattaaag acgcaatata ttatgactcg tataaagaag atcgcgattt
     7741 aatactggaa tataaccaga cttcacttgc agatgttaat ttagtcaata tccttggcat
     7801 aggtattgat aattgtacgc gtcagcaggc agtggtcaag gtgttgcgta tgatcgaaga
     7861 aggcggtgtg catcatgtaa tggcgttaaa tccatacaaa cttcaccgtc ttattacaaa
     7921 caatgactta aatctcattg cctcaaaagc tgatttaaaa atagctgttg gggcagggct
     7981 cccatgggca gcatcatttt taggcaaacc tttaaaagag cgaatacatt ttgtgagctt
     8041 tttaatggat atcatccgca ttgcagaaat aaaagagatt acaatattca tggtaggcgc
     8101 aaaaccagaa attattgaac aggcatatgc caacatcaaa aagtcattcc caatgatacg
     8161 cattgtgggg cgtcatggcg gttacttcaa taaagaaaga gcttccaatg tcattgaggc
     8221 aatgcgcaaa tccgaagcac agattatact ggttggactt ggtttcccca aagaagataa
     8281 atggatttat caaattaaaa atcagtttaa gaatactgtg tttattggaa tcggtggaag
     8341 tattgatatt atctccgggc aaaaacataa agctcccgct ttttttatgg aaaaaggcct
     8401 tgactggttt taccgcatta taacccgtcc atggcgttat ctgcgtttat tgcgattgtt
     8461 attattttat atacaggtag tatggcaaaa aatacggcaa taactatctt tcatatttac
     8521 ccggtttagc ttttgtatca cgtgtttttt gtaattctga tggtgtaacc tgacatcggc
     8581 ccagcgtaaa ccattccact atttcttttt tgccacaggt acggcagtgg aatacagcct
     8641 ttttatcctg ggtccactca ttcacttcaa cgtatcggga accgcaattc ctgcacaaaa
     8701 tcactgaaac atattccatc tgatgcctct tttttttatt ttacttttac agcaatgacc
     8761 gatatatcct cacgttgagt taaaccctgc ataaaatttt tgacagtatc aagaagtcca
     8821 gcaatgcaag attccggagt tgaaaattga gaacatgtgt ccatgacctg ctttaatcca
     8881 aaaagtcgtg cattacgatt tttgcaattt acaattccat cagtgacaat gacaaccata
     8941 tcctcatgct taagttttac aacaatcttt ttattattat atagcgaatg tgaaaatcgc
     9001 aaataatgtg caatactatc ccgtataata acaggatatg cctcgcctgt attcataaat
     9061 gttaaagttt ttgttttatt atcaatcata cccaaaaata acgatacatt attgatatga
     9121 tgtgaagcaa caacctgcag cgtatttaaa aagattggtg ccacaactct accataaaca
     9181 ttcattaatc ctttcaacaa accattaacc aatgcggcat tcagcgctcc gggaactcct
     9241 ttatcatgta catcacatac tgcaattgca actgcatcat catatggttt tatttcataa
     9301 aattcaccac ccacctcacg cgcaggaaga taacgagcag ctatctcaca aaaaacataa
     9361 tcatttgcaa tagatggatg caatgaatgt tgtatctcat gagcagcaga aagctcatta
     9421 cgcatgcgcg cctcttcaat agcccgctca aagaatattg cattctgcac agccagcacc
     9481 gcctgatcag caaatgctct gaacaaattc atatcctcat cagtgaaatc aggacggttg
     9541 attggattga ttgcctgtat cacgcccaga agctttcctt tatacaaaag tggaacacat
     9601 attattgaat tagtggtaaa tccagtctgt ttgtcaaaat tggggtcaaa gcgttcatcc
     9661 agataaacat cgttaacata cacgcctttg cgctcttttg ctacccaacc tgcaataccc
     9721 tgccccatcg caaccctgta tttctcctgc aatttatcac cggcctcacc cagagccact
     9781 ttaaacacaa gctcattggt tttctcatca aacaaaaaca aggtgcttgc ttcggtctcc
     9841 attatctctt taatgatttc cattattatg gtaaaaagtt ttgccatgtc aagtgttgag
     9901 ttaataatcc tgttaatttc aacaagcttt gacaggttaa agagctgacg ttctaagtat
     9961 gcaacgttgt acttttgttc gttgttacta tcagtcatac tatccctttt tcaattgtta
    10021 tactgtatgt cggtgttaag aaagctgtac tatacctttt ttatattttt atacattgga
    10081 ttatgcatca gtcaactaaa ttatacaaaa attttatatg gcgatagtta cttaaaaaaa
    10141 atgcttgtat agtactatag tttgtatata gtgtgaaaat cataatttcc agttaataaa
    10201 ccagtttgca agagtgtgtc atgacgtatc tgattgacgg ctataacctc atatataaat
    10261 ttccccattt agaagaactc atgcttcagg ataatctcat agaagcacgc cgtgagcttt
    10321 tagatttact aaagatgttt gccaaactaa cacgcaaacg aatacgcatt gtttttgacg
    10381 gacaaaaaac cagtgatatt cctatctcaa gcgaaaaagt atatactatc gatgtgtact
    10441 attccctgca ttattctgct gactttttaa taaaggaatt tatacgcaaa gacttacagc
    10501 cacgcaacac aactgtcgta accagcgaca aagatataat agagtatgtt agccgtttta
    10561 aaacaaaaac tatgaaaagc gaagactttg ctctgtttgt taatcaaacc attgaagatt
    10621 tcatctctgc tcaaacccct gaaaaagaag aaaatcccaa cctgagtcaa aaagagattg
    10681 aatactggga aaggcttttt accagcaaaa agaaataact aattttcagc agccagtatg
    10741 gctgcaccaa gtgcaccaat aacctgggca tcctttgaaa cttttaccgg ggaattcagt
    10801 aacttttcaa ttgcattgac aacgccaata ttttttgcca caccgccagt catcataaca
    10861 ggactggcaa tccccacacg cgatgccatt gccattacac gagatgcaat tgactggtga
    10921 ataccagcaa taatgttatc acgcgactca ccctttgcaa taagtgaaat tacttccgat
    10981 tcggcaaata ccgtacataa gctgcttatc ttggacggat tatttgcctt gagcgatagt
    11041 tcaccaaaca catccagatc aacctccagt gcacgtgcca ttacttctaa aaaccgtcct
    11101 gtaccggcag cacacttatc gttcatggca aaatccttta ccctgcccgt ttcatcgata
    11161 actatcacct tgctgtcctg cccgccaata tctataatag atcgtactga gttatctaag
    11221 aagtgagctc ctctggcatg gcaggtaatc tcggttaccg tagcatgtgc aaatccaaca
    11281 cttttccgac catagccggt tgctacaatc ctttcaattt gttctagtgt tagccctaat
    11341 tcagctaaaa gctcctgtac caccttttcg ccagcttttg cagcattaaa ccctgtaaaa
    11401 attaccttac tgccaactat agtgccacca tttaaaacaa cagcctttgc agtaattgaa
    11461 cctatatcca tacccagtgt aagcatatac catccttttt ttataaaaca agcgttcgct
    11521 cattatttgt caagctttta ttcaccaatc aggcattttg ccgttttctt tttctcttcc
    11581 tattatgtcc atcttatatt ctaatggcaa ataatatata attgccctgg cagaaaggtc
    11641 ctcttttttt accaatccaa caaccatcat ttcagggata cttccctgct gtataaatgt
    11701 cctgaactgt accggcaatg acatctcagt aaaatgccat cgtgcaaact gcttattggt
    11761 aaacatagtc tgtatgcgca gcattacctc atctactatt gtatcgcaaa cagttgattt
    11821 tagcatactc tcggttgatg cagtatcact gaacgctttt gctgattgag aaaaaatgaa
    11881 ataatcctca agtgcatttt catctattcc catacatcgt gcaaaatcag ccttactata
    11941 aaaattatga gcgttatgca ttgtgcatag catggctcca ctgcggacac catgcttact
    12001 gcaacacaat gccaccaata caatgataaa aggaactatc gccctgaaat ttttcatata
    12061 tttttttatt ttaaattttc attaaagaat gccatcatat cgggaagcac cgactgacag
    12121 taattccaat catgcatctt atactttttt tccacaaatg taaaattata gccactttta
    12181 tattcctttt gaagctgttt caactttaac gccagcataa acgcctgctc catgggaaca
    12241 cgcgaatctt tattgccatg tgcaatgaac actgcaacat cgccaagatt ttttgcttca
    12301 tcaatagcat tatcagtttg tgcccatcgt tgttgaaagt cattatattt tccatagacg
    12361 gcggtaaaca tatgattttt tgtatgcgaa agcatatcgt aatatcctga caaacatgca
    12421 atagcgccaa caaagtcatg atacacctca cccacgcgca atgcaccacg cgcaccaatt
    12481 gaaaaaccac acacgccaag tttttgcttt gtaatgttta agccaacagt attatttaaa
    12541 tagggcagca gtaactggcc aatccacaaa agcgccggca tctcattcca tttaaccgta
    12601 gtttgatcaa aataatatga ttcgtaaact gagcgtggca tgtgtgggca cacaatgacc
    12661 atattgtatt gattggcata ataggcaatg ctggtgttat tctgccagct attcatagaa
    12721 ccgtcatatt catgaaatgc gataataaca cggcttgaat ctgaatagct tttaggaaag
    12781 taaatcttaa ccggtattgt tttttcggat tgtggtgaag cgggcacatt atagataacc
    12841 catgtaccat gcctggtatc agaaacaagt gttgcgtgta taaaaattac ggtaatggca
    12901 atcaacacaa tagtacccgg ttttttgtat ttcatatgtt ctcctgatta tttattcata
    12961 ggaccaaaat cccggacgct acatgctgga ttgctttcat agttggttct tccgggatgt
    13021 atggcaatac tcctatgact gttccataat aaagtcatta tttatggtat aaatatttaa
    13081 aactgctgtc aactgtttta ctatatcctt tctatgtaac tgtatgatta tattttaaca
    13141 aagcctactg accaccgctt tatatatagc attatgccaa aaataacttt tcatcagttt
    13201 cacgcaaact cagataatcg ttatcggtaa caattacatg atccagaagc tgaatcccaa
    13261 ctatagcacc aacctgtgct accctgcgtg taatctcaat atcattgacc gatggcttaa
    13321 gcaaccctga cggatgattg tgagcaatga taatggatga tgctgcttcg cggattgcat
    13381 ctctgaagac ttcccgagga tgtactattg attcagagat agtacctatg gaaactcttg
    13441 tttttttcag caatctgttt ttattatcca gtataagcgc ccagaattcc tcctgcttat
    13501 tgcatgcaat ctgtcccaca atgaattccc ataccatacg cggtgagtca attgaccctt
    13561 cataattttt tggtactatt aacctcctcc ctaactcgaa tgcagctttt atacgaattg
    13621 ctttcacaaa tccaatacca tctgtatgtg ccagttctcg tatccctgca tgatatatcc
    13681 cgttcatccc tccaaattgt tcaatgacat aaaacgcaca ttccataaca tttttccctt
    13741 ttaccccact gccaattagt actgctaaaa gctcaacatc gctcaatgct tcaagtggtt
    13801 caccttttat acaccgttgt ataggcaaat cattttttga ttccatagta tcctcctcag
    13861 aaaatcgaaa tattaacctt accccctgtc ctttgcaaaa cgagaggagc attgttcggt
    13921 ttgtataatt ttcatttttt atggatggca agatgcttac catcatgaat tccgtaaaac
    13981 ataatgttgt tattatttac attccaacta tttcaaaagt aactatcgcc catatataaa
    14041 aacgccggaa tggtaatttt taaaaaaaat ttttttttaa tttatatttt ttttcaatta
    14101 ccgatagtta tagtaagatt ttttatgtca cattaaaatt aactgttgac aaaaaattat
    14161 aggtagtgaa tactgatgac cataagggag tttgccatca gggagggata tatgaaagta
    14221 agtattgatg ctattctgca atcagcaaaa caattaaaaa ataccaacag tgttgatgaa
    14281 caaaagtcgg gtaacaaaca taaagtatcc aatgataaag tttcccttga gcataaagtg
    14341 cttgcacgcc ttgatgattt acacaatcag atcaaagata ttcaaactac actttccaga
    14401 tatcaggtta tcgatgaagg aatacggctt atcattgaag ataataaaaa tggtgggaat
    14461 aacaagcagt ccattattaa ttcaactctt tataacaata gtcctgtttt aaaagaatat
    14521 cttggtgata gttccactaa tgagtatata gtgcataagc aggaacaggt aaaaaatctt
    14581 attaccgatt ccatatctca tcttaacaga ttacaggtag agtttgaaaa tatcatggct
    14641 tcggatgttg cacatacccc ctatataaaa gataaggttg atgcaatcga tgcacttatc
    14701 ccaagaaatg tgaaaaatcc atcacagctt catcatgtac attccgattc agtgttgcgt
    14761 ttgattcaat aaaactgtta taccattaaa aaaacgttcg taacgtataa tgctctgacc
    14821 ccttttttta tacccattgt gcaatggagt aacgcttacg gtacgcctcc tcatactgca
    14881 aaaaacgtct cactcgttca ctgaaactgt ctcccagctg cagaaagtac tcatgatgat
    14941 ctatcggtac accaacatca gcacc
//
LOCUS       DAOSOX010000038        14928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4028_length_14928_cov_10.382371, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000038 DAOSOX010000000
VERSION     DAOSOX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_14928_cov_10.382371"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..772
                     /locus_tag="PLX22_04440"
     CDS             314..772
                     /locus_tag="PLX22_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19177.1"
                     /translation="MKRIVGVVAVVVVVFVFIAADKTPSAEELIDVLGLRHSKVFCKY
                     GVPVKMYVDRGPTKEEDYIIFDYLQFGLVFHHEMIRQILFYPEWKGGKIRKIDIGMSK
                     DEVIKIMGEPQEEVALSDGRTRITYLEPDKYFDVILSTSNVVNMIRFEVK"
     gene            800..2464
                     /locus_tag="PLX22_04445"
     CDS             800..2464
                     /locus_tag="PLX22_04445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19178.1"
                     /translation="MSFEVPVSLVQFLLPPMCFGLLIGGFILYVYMYWKYKNSLYFAI
                     AFFALLGTLFVGSETMILSFGGWLHNRSVSVHFHRLEQTAGIFYLFALPYSLNKLLIL
                     NKRFFKINTILAYIGLAYAVIATIGAFVFPDLFISLTKHKATWMYYEADYGRGQEGIL
                     YAIRDVLLGIYIIYSIVAVMIDMILHRNYRYLLYPALGLLFAFAGAAIDTLHVHTQVF
                     YDLFPNVYFSRFSVGITLAIMFFMSSVTSMFINYAQELEIAHKIISISEKKYRFLVEG
                     TNDCILSFDSNLNITTANRAAYEQLAVRRGLESATSFYDLVYLPEGESDLTLQIIKEK
                     INMLFTEGNPINLKIQLKSRGTLEPKEYTIRFEHIVIDDRNEILMKATSVLDENILKY
                     IEAEKLKLSIGNYLIAAEEICNRLVLNLPKYLDKKTTNTIKLGLREIIINAIEHGNLN
                     IGFEEKTQATNNGNYFEFVLSRQKDPNYKDKKVTIELLLNCNKVMYKIEDEGNGFNYR
                     DIIQKIQNTVDDDMLAHGRGLRMVYNIFDEVKFNNKGNQVLLVKNF"
     gene            2477..>2935
                     /locus_tag="PLX22_04450"
     CDS             2477..>2935
                     /locus_tag="PLX22_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT19179.1"
                     /translation="MKKPHVIVRRCDDYDMSAISAIINESVNDLKLKIPIQGNVFIKP
                     NVVTANKQYIFNSYTHPAVVEAMVTVLKDKSINNIIIGESGGYGIPSRLFLKEAGYFD
                     LAEKLNVPVVDLNEHPVVKVKLSKAKWHKEIELSKYIAEANFKIWMPKLKY"
     assembly_gap    2936..3035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3036..3668
                     /locus_tag="PLX22_04455"
     CDS             <3036..3668
                     /locus_tag="PLX22_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19180.1"
                     /translation="ILSNNPLACDVVASYIMNYRPQDVLHLQLAHERGYGSLQLKDIN
                     ITGDISIDKLRAKPKGQRKLFQVLQELDTPLTFYSGYAPNTDIICDGGCEAAIKGCLG
                     TIEKRRPGSLKKAKKGAIVTGIYKGDIVVPDGNVLLVGDCTKVDGKLVAKRVMRIKGC
                     PIGARNLFIPVPLLFGLPSPMFDIRDAFLFIVNSIQKVFNIVKYRWVLKQ"
     gene            complement(3755..4414)
                     /locus_tag="PLX22_04460"
     CDS             complement(3755..4414)
                     /locus_tag="PLX22_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT19181.1"
                     /translation="MKSNIKSKDYQNFLDDLKGYYQENEQKEEENIPVGTRIRQIRQM
                     QGVDSATLAKNAGIDHKYLEDVESLAIYPDLGTIIKITNALKVSTGSIFDLDSSRSYT
                     IIRKIDRKKIMRSISGVKEHPDYEYLSLSSGVISRHMEAFIVTLKSNVYYTEELSTHE
                     GEEFLYMLEGAMKIKLDDKEETLQQGDSIFYHSTIPHVLKSISPKPAILLAVLYTGYS
                     S"
     gene            4630..6042
                     /locus_tag="PLX22_04465"
     CDS             4630..6042
                     /locus_tag="PLX22_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT19182.1"
                     /translation="MVTIFKKQNLRIHMIGICGVGMSALAGMLQSQGHMVTGSDTDVY
                     PPISTQLEKLQIPVCKGYSSDTPGKIKPDIVIIGNAISRGNPEVELVLNNRIPYFSMA
                     GALYNFFLYDKEVIGVAGTHGKTTTTSLLAYILEQADMNPSFFIGGIPKNFETNYKVG
                     SGRYFVIEADEYDSAFFEKMPKFIMYRPVHCIMTSLEFDHADIYNNIDEIAVWFTRLR
                     NLIPANGYIVYNNAYSNLSTINNDALSSTVSYGKMSADCCYRYQNEVLTITMDDMAIN
                     VTPHIYGDFNHQNITAAVAMALTLQVPVGAIVMAINSFQGVKRRQEILFSNDYLTIIE
                     DFAHHPTAISNVLTQVSEHYSDAAIVAIYEPRSATSRRNVFQDELPLAFSKAHKVIMK
                     RPYRLDKVPAHEQIDIDRVINKLCQMQIPACVLGSVNEIVQEAFSGLSNKRTVMVVMS
                     NGGFDGIYELLLAHTKNVFA"
     gene            6333..7697
                     /locus_tag="PLX22_04470"
     CDS             6333..7697
                     /locus_tag="PLX22_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT19183.1"
                     /translation="MAKDSTKIQLPEREMPRQWYNITADLPKPLDPPISPATGKPVTP
                     DELHAIFPMGLIEQEVSSKQYIDIPEEILHIYRLWRPSPLFRARRLEAALGTPATIYY
                     KHGGVSPAGSHKLNTAVAQAYFNKKEGVKKLSTETGAGQWGSALALAGNFFGIDVHVY
                     MVRVSYEQKPYRKFMMETWGAKVTPSPSNITNSGREILKQDPESPGSLGIAISEAVEE
                     AAQSKDTNYSLGSVLNHVCLHQTIIGLETLKQFEMVDDYPDVVIGCVGGGSNFSGLAF
                     PFVRDKINGKDINIIAVEPSACPTLTKGIYTYDFGDVAKLTPIMPMYTLGHSFIPPSI
                     HAGGLRYHGMSPLVSYLYHEGLISAMALHQNECFEAAILFSKTEGIIPAPESSHAIRA
                     AIIEAQKAKEEGKKKTIVFNLSGHGHFDLASYDKFLSGQLEDYDYPRELIDKALHDLP
                     KVVK"
     gene            7886..9340
                     /locus_tag="PLX22_04475"
     CDS             7886..9340
                     /locus_tag="PLX22_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOT19184.1"
                     /translation="MKRIVFIILLCFMSCSQKQYQDYIPNTAQCIYDESHNNSWRFFD
                     HGYCGYSTIAQFLKNYGIPPSITIKHLDECIQQCYRSGYTNTLLILSVAKYQQFDSGE
                     VDALCNFVKKGGLLFVIAEHENMYNSAQFLNAVTTKLGIQINNDAAGNGSTPLQSIDN
                     LNNSAYSKVFTLKNVLHMLAASLSGMNKTNSSFEILLCDDTTDKVIAAGCIYGKGRML
                     VVGDSEMFWNGDGKVGINAGDNRAFFKSCVEWLLKKRLNKIVHHTTNVRCFDPENSMV
                     QIDNSCSGIQCFMDALKEKIPSITTDSNITIVVHPQPQFTVKEGKRTIVFIEPYQNLM
                     SSTVWGNQLLKAGAVNPSVYVSFAKSAKIQILPAFITNGDSNYYMVTIAHDKNSLVFN
                     SLAALLSHRSDGMLFKIPSAFWGEVAHPGLEVINDGIPMYQSSDYTDAGYMYADSKLL
                     IVGDADAIANQNNNTQTFSAIVTMVTNWIQKGSL"
     gene            9427..10191
                     /gene="hisF"
                     /locus_tag="PLX22_04480"
     CDS             9427..10191
                     /gene="hisF"
                     /locus_tag="PLX22_04480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT19185.1"
                     /translation="MKTITIMPCLDMKEGRVVKGVNFINIKDAGDPVENAVYYQHEGA
                     DELAMLDIAATVENRKTRLEWVKNVSGVITMPLTVGGGIRSIEDIELVLEAGASKISM
                     NSAAVKNPDSIDEAAKRFGTEKITIAIDGKRNTAMPSGFEVVVAGGTISTGKDVVAWA
                     IECQSRGASVILPTSMDGDGTLQGYDIPFTRAIAEAVNIPVVASGGAGKLEHFYEAVV
                     EGKASILLAASVFHFRTFTIKQVKNYLRGRGIAVNL"
     gene            complement(10199..10269)
                     /locus_tag="PLX22_04485"
     tRNA            complement(10199..10269)
                     /locus_tag="PLX22_04485"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10235..10237),aa:Gly,seq:tcc)
     gene            10572..11480
                     /locus_tag="PLX22_04490"
     CDS             10572..11480
                     /locus_tag="PLX22_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19186.1"
                     /translation="METTFYSLATKTNFNKALNHFYRVNDVVDEIESGRNFADAVNVM
                     LAEGDLTVDQVLPMIGAIIKDKYQYSYDSYNVPVTINDFSKIVEETSKWTALDIILVY
                     FTVNSEPFLINPKNMIHWERVRDIVRDQLMVIYVKKKKEPNPKVENDAINAIEEMMAG
                     KDVFINKEFIDETVAVREAKKQIAPAEAAAAGKKNMTPKYAVTVTNELFHNGNVEAWK
                     RIIESFKVSHPDLDVLIFHEGEKINDINSLFKWGKVKHGDPILFQVVGENIRNVSKLQ
                     KYLFEGASPRFEQFLKIAVGKVLNLF"
     gene            11519..14119
                     /locus_tag="PLX22_04495"
     CDS             11519..14119
                     /locus_tag="PLX22_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOT19187.1"
                     /translation="MENIVFFNKDFTGVDKKQYKELGIRGRRAADLAKMGLPIAPGFI
                     VDAELTMKLPKVNVKQLIKSYVDKIEKDMKKGYGDTEKPLLLKVVLSSDLNVPFFPSI
                     HNVGLNDAIVTAFAKFTGEDFAWGEYRFLLRSIASKIYGIDEKELDELEGKLPKNAKA
                     KEYKEVVTKYKKFLGDKFSEDVYDQLGLILKGAAARYCDSDIDVDDSLAITIQAMVYG
                     NFGQNSFAGNYFTRNIITGDALIQGDFLQNEFDVDRGTPKDIAKIDKKYYDKLVEIAK
                     KVEDNFKEIRNIKFTIEEGNFWLIEQREVDEKSTQAHIKTLLDLNTRKVVSSNFLIES
                     IKPSQLNELLHPVIDPRTTKNVKSIKGGIAGSTGAAIGRVFFSTDKLLEEYKRSVMHG
                     GDTNLILVMPATFAEDVKAIEVARGVITSEGGFSSHAPVVARSLGKVAMVQPEIKIRG
                     NSFTLAGKTVKEGDYVSINVPYYEPPTIYLDKVGLIEPDFEKNGLIDFLKIVQSFIKD
                     FDVRANADQPRDAKVAKMFFADGIGLCRTEHMFFNEKRIMKFREMILSETEVERRKAL
                     DELKPMQRSDFYELFKIMQGHPVTIRLLDAPLHEFLPRTDDSMKDFIQYMKSRKKGMS
                     DAEIRIRCEELSEMNPMLGHRGCRVAITYPEIYEMQCRAIFEAACMLKKEGIDVVPEI
                     MIPIVMSETEIKFIKNGKRIEGKTVKGIRDIKDEVVEEYGIDDLEYEVGTMIELPAAA
                     VGAGRIAQYAEFFSFGTNDLTQTTYGLSRDDINSFFPSYSLYDLIPNNPFKVLGREVK
                     ELIEMASTRGSLTRPDITMGLCGEHGADPDNIEFCMNVGLNYVSCSPYAIPLSKLAIA
                     QLNIKNAKSK"
     gene            complement(14570..14773)
                     /locus_tag="PLX22_04500"
     CDS             complement(14570..14773)
                     /locus_tag="PLX22_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOT19188.1"
                     /translation="MLTPEELELYQRQLIIKGWGKESQEKIKKTHIFIAGAGGLASTA
                     SLYCAVAGFGTITLCDYDTIIHS"
BASE COUNT         4748 a         2317 c         3110 g         4653 t
ORIGIN      
        1 atatggttat tgtattaata ggttttttta actgatgact aatgaacaaa gttctttaag
       61 ggtattgtac catcccacca tgggtactga tgattagtat atccaatcaa taaacgggcg
      121 attacattca ttatatcaaa aaattatata catatgctgc tgaaaactat tgtataccat
      181 aggtaaaatt attttaaaag cgacctgaaa aaaatataaa aaaattaata acttgcaatt
      241 ttccaacccc tgtgagtatg tttttatcat tattatcatt aatggtacaa tattatttca
      301 aaggaggctg tatatgaagc gtatagtagg ggttgttgct gtagttgtag ttgtttttgt
      361 tttcatcgcg gctgacaaaa caccttcggc agaggagctc atcgatgtgt taggactacg
      421 tcattcaaag gtattttgta aatatggagt tcccgttaaa atgtatgttg acagaggacc
      481 aacaaaagaa gaagattata tcatctttga ttatttacag tttggtttag tattccacca
      541 tgaaatgata cgacagatat tgttttatcc ggaatggaaa ggtggaaaaa tcagaaaaat
      601 tgatattggt atgagcaaag atgaggtaat aaaaattatg ggggaaccac aggaagaggt
      661 tgctcttagt gatggccgta cacgtattac ctatttagaa cccgataaat actttgatgt
      721 gattttgtca accagcaatg tagtaaacat gattcgcttt gaggtaaaat agtttagttt
      781 tattatagtg gggaagagca tgagttttga agtgcctgta agcctggttc agtttttatt
      841 accgccaatg tgttttggac tccttattgg gggatttata ttatatgtct atatgtactg
      901 gaagtataaa aattctttat attttgctat tgcttttttt gcactgttag ggacgctttt
      961 tgttggtagc gagacaatga tactgtcatt tggtggatgg ctgcacaatc gctcagtatc
     1021 tgttcatttt caccgattgg aacagacagc agggattttt tatctttttg ctcttcctta
     1081 ttcattaaac aaattactga ttttaaataa gcggtttttt aaaataaata ctattttagc
     1141 atatattgga ttagcttatg ctgttatagc tactatcggt gcatttgtat tccctgacct
     1201 ttttatatcg ctaacaaaac ataaagctac atggatgtac tatgaagctg attatggacg
     1261 aggacaggaa ggcatactgt atgctattcg tgatgttctt ctgggtatct atataattta
     1321 cagtatcgtt gctgtgatga tagatatgat tttgcacagg aactatcgct atttgttata
     1381 tcctgcgtta ggtttgttgt ttgcatttgc tggtgctgcc attgatacct tacatgtaca
     1441 tacacaggta ttctatgacc tcttccccaa tgtctatttt tcacggttca gtgttggtat
     1501 tacacttgca ataatgtttt tcatgtcatc tgttacaagt atgttcataa actatgcaca
     1561 ggaattggag atagctcata aaataataag catatccgaa aagaaataca gatttcttgt
     1621 tgaaggtacc aatgactgta tattatcatt tgattctaat ttaaatatta ctacagcaaa
     1681 ccgtgcagca tatgaacagc ttgccgtcag gagagggctg gagtctgcaa catcatttta
     1741 tgatctggtt tacttgccgg aaggtgaaag cgacctgaca ttgcagataa ttaaagaaaa
     1801 gataaacatg ctttttaccg agggaaatcc catcaattta aaaatacagc taaagtcacg
     1861 cggcacttta gagccaaaag aatatactat ccgctttgag catatagtga ttgatgaccg
     1921 aaatgaaata ttgatgaagg caacatcagt tctggatgag aatatattaa aatatattga
     1981 agctgaaaag ctaaaactat ccattggaaa ttatctgatt gctgctgaag agatatgtaa
     2041 ccggttggtt ttgaatttac caaaatatct tgataaaaaa actacaaata ctattaaatt
     2101 aggtttacgt gaaataatca tcaatgccat agaacatgga aatttaaata ttggctttga
     2161 agaaaaaacg caggcaacca ataatggcaa ttattttgaa tttgttttaa gcaggcaaaa
     2221 agatcccaat tataaagaca aaaaagttac tatagaatta ttactcaatt gcaacaaggt
     2281 tatgtataaa atagaagatg aaggtaatgg atttaattat agagatatta ttcaaaaaat
     2341 tcagaatact gttgatgatg atatgctggc acatggaaga ggtttgcgca tggtatacaa
     2401 tatttttgat gaggttaaat ttaataataa gggcaatcag gttttgctgg tgaaaaattt
     2461 ttaaggagct aattatatga aaaaaccgca tgttattgta cgaagatgtg atgattatga
     2521 tatgtcggca atttctgcta ttataaatga gtcggtgaat gatttaaaat taaaaatccc
     2581 cattcaggga aacgttttta ttaaaccaaa tgtggtaaca gcaaataaac aatatatctt
     2641 caactcgtat acccatccgg ctgtggttga agctatggtt actgtgctaa aagataaaag
     2701 cataaataat attatcattg gcgaatcagg tggatatggc ataccttccc gactattcct
     2761 taaagaagca ggatattttg atcttgctga aaagcttaat gttcctgttg tagatttgaa
     2821 tgagcatccc gttgttaaag taaaactttc taaagctaaa tggcacaaag agatagaatt
     2881 atcaaaatat attgcagaag ctaacttcaa aatatggatg ccaaagctta aatacnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatact atccaataat ccactggctt
     3061 gtgatgtggt tgcatcgtat attatgaact atcgcccgca ggatgttttg catttacagc
     3121 ttgcacatga acgcggatat ggctcattgc aattaaaaga cattaatatt accggggata
     3181 taagcattga taaactacga gcaaaaccaa aaggtcagcg taaactgttt caggtactgc
     3241 aagagcttga cacgcctctt acattttatt cagggtatgc acccaatact gacattatct
     3301 gtgatggcgg ttgcgaagca gctattaaag gttgtttggg aactattgaa aaacgaaggc
     3361 ctggttcatt aaagaaagca aagaagggcg caattgttac cggtatttac aaaggtgata
     3421 ttgttgtgcc cgatggcaat gttttgcttg tgggagattg cacaaaagtt gatggcaagc
     3481 ttgtggcaaa acgggtaatg cggattaaag gatgtcctat tggcgcacga aatcttttta
     3541 tacctgtgcc attgctgttt gggttgccca gcccaatgtt tgatatccgt gatgcattcc
     3601 tgtttatcgt taactctatt cagaaggtgt ttaacattgt taaatacaga tgggtgctaa
     3661 aacaataaac aatgctgggc gatagttact gcttattagt tttgcactga taaaataatt
     3721 acactgccat attacaattt gctgtatact aaagttatga agaatagccc gtatagagga
     3781 ctgcaagaag tattgcaggt tttgggctga tgcttttcag cacatgagga atggttgagt
     3841 ggtaaaaaat gctgtcgccc tgctgcaatg tttcttcttt gtcgtctaat tttatcttca
     3901 ttgctccttc cagcatatat agaaattcct cgccttcatg tgttgataat tcctctgtat
     3961 aataaacatt acttttcaag gtgacaataa aagcttccat atggcgggat ataacgccgg
     4021 aggacaatga aagatattca taatcagggt gttcttttac tccggatata cttctcataa
     4081 ttttttttct atcgattttt ctgattatgg tgtatgaacg acttgagtct aagtcaaaaa
     4141 ttgagccggt tgaaactttg agcgcattgg ttattttaat aattgttcct aaatcggggt
     4201 aaattgctaa tgattcaaca tcctctaaat atttatgatc aataccggca ttttttgcta
     4261 acgttgcact atcaacaccc tgcatctggc gtatttgacg aatccgtgtg ccaaccggta
     4321 tattttcttc ttccttttgc tcgttttcct gataataacc tttcagatca tctaaaaagt
     4381 tttgataatc tttgcttttt atattggatt tcattatgcc ctcactacta tatagtggat
     4441 tgaaatacta tacgttactg cagcattgtc aacaatattc ttacattttg gcaactataa
     4501 atgttttgca cctgtaaaac aagctttaca agcaacgatg tttactaatg attgtgttgt
     4561 acttataata agtggtttat atggtagcaa tttgttatgt aaattattta taaaagagga
     4621 tgttaatcaa tggttactat ctttaaaaaa caaaatttac gcatccatat gataggcatt
     4681 tgtggagtgg ggatgagtgc gcttgccggt atgctgcagt cacagggaca catggttact
     4741 ggctcggaca ctgatgttta tccaccaatt agtacacagc ttgaaaaatt acagataccg
     4801 gtatgcaaag gatacagctc tgatactccg ggaaaaatta aacctgatat cgttattata
     4861 ggtaatgcta ttagccgcgg taatcctgag gttgaactgg tgctcaataa ccggatacct
     4921 tatttctcga tggctggtgc attatataat ttttttcttt atgataaaga agtcattgga
     4981 gttgcaggaa cacacgggaa aacaaccaca acatcgttac tggcatatat acttgaacag
     5041 gctgatatga atccatcatt ttttattggc ggcatcccta aaaattttga aacaaattat
     5101 aaggttggca gcggcaggta ttttgttata gaggctgatg aatatgattc ggcatttttt
     5161 gaaaagatgc caaaatttat tatgtatcgt ccggtgcact gcatcatgac atcgcttgag
     5221 tttgatcatg ctgatatata taacaatatt gatgagattg cagtatggtt tacacggttg
     5281 cgcaatctca ttcctgcaaa tggatatatt gtttataata acgcctatag taatttaagc
     5341 acaataaata atgatgcgct atcctcaacg gttagttatg ggaaaatgag cgcagattgt
     5401 tgttatcggt atcaaaatga agttcttacc attacaatgg atgacatggc aatcaatgta
     5461 acgccacaca tatatggcga ctttaatcac cagaatataa ctgcggcggt tgctatggct
     5521 ctgacccttc aagttcctgt tggggcgata gttatggcaa tcaattcatt tcagggagta
     5581 aaacgcaggc aggagatact cttttcaaat gactatctaa caataataga ggattttgcc
     5641 catcatccta ccgctatcag caatgttttg acacaggtgt cagagcatta cagcgatgct
     5701 gccattgttg caatctatga gcctcgctcc gcaaccagcc ggcggaatgt atttcaagat
     5761 gagcttcctt tagctttttc aaaagcacat aaggtaatta tgaaacgacc ctatagattg
     5821 gataaggtgc cggcgcacga acagattgat attgataggg tcattaacaa actatgccag
     5881 atgcaaatac cagcctgtgt tttagggagt gttaacgaaa tagtgcagga agcatttagt
     5941 ggattgagca ataaacgcac ggtcatggtg gtaatgagta atggtggttt tgatggcata
     6001 tatgaacttt tgcttgctca cacaaaaaat gtatttgcat gagtaaattt taaaaaaaaa
     6061 atttcttgac attacataat tgttttatct aatgctatca agtcatttgt tgaaattgta
     6121 ttatatatga tgattaaaaa atgtaacatc gtgttttatg tggtatggtg gtggcgccag
     6181 gattacatat atccgggcga gtaaggaagt tttttacata tgcaaatcct tgaaggctac
     6241 ggatgctata tgttaaccgt agctttttta ttttatataa gaaaatgcat attttaaatc
     6301 tgattattta aatttattgt aaggaggatt tcatggcaaa agattcaacc aaaatacaat
     6361 tacctgaaag ggagatgccc cgtcaatggt ataatatcac agcagatctg ccaaaaccgt
     6421 tagacccacc cataagtcct gcaacaggga agccggtaac acctgatgaa ttgcatgcga
     6481 ttttccccat gggactgatt gaacaggaag taagctcaaa acaatatatt gatatacctg
     6541 aagagatatt gcacatttac cgactatggc gaccttcacc actttttaga gcacgaaggc
     6601 tggaggcggc attgggcacc ccggcaacta tctattataa acacggaggt gtttctccgg
     6661 ctggcagcca taagttaaat acagctgtgg ctcaagcata ttttaataaa aaagaaggtg
     6721 ttaaaaaact atcaaccgaa accggagcag ggcaatgggg tagtgctctg gcacttgccg
     6781 gtaatttttt tggtattgat gtacatgttt atatggtacg ggtaagctat gagcaaaaac
     6841 catatcgtaa atttatgatg gaaacatggg gtgccaaggt taccccaagt cccagtaata
     6901 ttaccaatag cggcagggaa attttaaagc aggatcctga aagccctgga agccttggca
     6961 ttgcaataag cgaggcagta gaagaagctg cacagagcaa ggataccaat tattcactgg
     7021 ggagcgtgtt aaatcatgtc tgcctgcatc aaaccattat aggtctggaa acattaaaac
     7081 agtttgaaat ggtagatgat tatcctgatg tagttatagg ttgtgttggt ggaggttcta
     7141 atttttcagg gttggcattc ccatttgtaa gggataagat caatgggaag gatataaata
     7201 tcatagcggt tgaaccgtcg gcatgtccaa cattaacaaa aggaatttat acgtatgatt
     7261 ttggggatgt tgctaaattg acgccaatta tgcccatgta tacattaggg cattccttta
     7321 taccgccatc aatccatgct ggcggattgc gttatcatgg tatgtcgcct ctggttagct
     7381 acttgtatca tgagggatta atcagcgcca tggcactgca tcaaaatgaa tgttttgaag
     7441 ctgcaatttt attctcaaag actgaaggta ttattcctgc tccggagtca tcccatgcta
     7501 tacgggcagc cattattgaa gcgcagaaag caaaggaaga aggcaaaaag aagactattg
     7561 tatttaatct aagtggtcat ggccattttg atttagcatc atatgataag tttttatcag
     7621 gacagttaga agattatgat tatcccagag aattaattga caaagcatta catgatttgc
     7681 caaaagttgt gaaataatga aagttataaa aatcagtgga tggtatagcg aggctgtttt
     7741 aaaacagcct tgttttttta tgaaccccgt aatgattgaa agtggaggca cattacaaag
     7801 cgcctgaaaa actttttgtt ttgacatttg cattctggat tttttattat gagttgttgg
     7861 acatattttt caatggaatg atagtatgaa aagaatagtt tttattattt tgctatgttt
     7921 tatgagctgt tctcaaaaac agtatcagga ctacataccc aatacagcgc aatgtatcta
     7981 tgatgaatca cataacaact catggcgttt ttttgatcac gggtattgcg gctattcaac
     8041 tattgcacaa tttttaaaga actatggcat cccaccgtct attaccataa aacatctgga
     8101 tgaatgtata caacagtgtt acaggtcagg atataccaac acgctattaa tactttcagt
     8161 tgcaaagtat cagcagtttg atagtggtga ggtagatgca ctatgcaatt ttgttaaaaa
     8221 aggtggattg ctttttgtca ttgctgagca tgagaatatg tataacagtg cacaattttt
     8281 aaatgctgta actacaaaac tgggcataca aatcaataat gatgcagcgg gaaacggttc
     8341 cacaccatta cagtcaattg ataatctcaa taatagtgca tactctaaag tatttacatt
     8401 aaagaatgtt ttgcatatgc ttgctgcttc attatccggg atgaataaaa cgaatagttc
     8461 gtttgaaatt ttattgtgcg atgacacaac tgataaggtc atcgctgcgg gctgcatata
     8521 tggtaaaggc agaatgttgg tggttggtga cagcgagatg ttctggaatg gcgatggtaa
     8581 ggttggtatc aatgcaggtg ataacagagc attttttaaa agttgtgtgg agtggcttct
     8641 taaaaaaagg ctcaataaga ttgtgcatca caccacaaat gtccgttgtt ttgacccgga
     8701 aaattcaatg gttcagattg ataattcatg ttccgggata caatgtttta tggatgcact
     8761 taaggaaaaa attccttcaa ttaccactga tagtaatata acaattgttg tgcatccaca
     8821 gccacagttt actgttaaag aaggtaagcg taccattgta tttattgaac catatcaaaa
     8881 tctcatgtct tcaacggtat ggggaaatca actgttaaag gctggagcag taaatccttc
     8941 ggtgtatgta tcgtttgcaa agtctgcaaa aatacaaatt ttgccggcgt ttattactaa
     9001 tggcgatagt aactattata tggtaactat cgcccatgat aaaaattcct tagtgtttaa
     9061 cagcctggcc gctttgctat cccaccgaag cgatggcatg cttttcaaaa ttccgtcggc
     9121 gttctggggc gaggtggcac atcccggttt ggaggttata aacgatggta tcccgatgta
     9181 tcaatccagt gattatactg atgctggata tatgtatgct gacagcaaac tattgatagt
     9241 tggtgatgct gatgctattg ccaatcaaaa taacaataca caaactttta gtgcgatagt
     9301 tactatggta acaaactgga ttcaaaaagg tagcctataa cagtttatat cttgactgaa
     9361 ttgtatgata cttcatacat gtgatatgag aagttgtata gtatgcaaaa taaaaggatg
     9421 gcacctatga aaacgattac cattatgccc tgccttgaca tgaaagaagg gagagttgtg
     9481 aagggtgtta atttcataaa catcaaagat gcaggcgacc cggttgagaa tgcagtgtat
     9541 tatcaacatg aaggtgctga tgagcttgcc atgcttgata ttgctgcgac agtggaaaat
     9601 cgcaaaactc gtctggaatg ggtaaaaaat gtttccggtg ttattaccat gcctttgact
     9661 gttggtggtg gaattagaag cattgaagat attgaactgg tattggaagc tggcgcatcc
     9721 aaaatatcaa tgaatagtgc tgcggtaaaa aatcctgata gtattgatga agccgctaaa
     9781 cggtttggta cagaaaaaat aacaatagcg attgatggta agcgaaatac cgctatgcct
     9841 tcaggttttg aggtagttgt tgcgggtggt accatatcta ccggaaagga tgttgtagca
     9901 tgggctattg aatgtcaatc gcgtggtgcc agcgttattt tacccaccag tatggatggt
     9961 gatggaacat tacaggggta tgatatacca tttacacgag ctattgctga agctgtgaat
    10021 attccggtgg ttgcttctgg tggtgcaggc aaattagaac atttttatga agcagtggtt
    10081 gaaggaaaag ccagcatttt gcttgcagca tcagtgtttc atttcaggac tttcacaata
    10141 aagcaggtga aaaattactt aaggggaagg ggaatagctg taaatttgta aataaatgag
    10201 cgggtgatgg gaatcgaacc cacgctatca gcttggaagg ctgaagttct accattgaac
    10261 tacacccgct ttaaaaccat atgcaccact gtggtgcgat cataccattt tgtcaagaat
    10321 ttaatacgat gataattgcc acctgttttc ttttcccact tatctttaga gcaattattt
    10381 acccttataa gattatacaa tgttaataag tccgaagcga ttgctcataa aataaaaaaa
    10441 atacttgtaa aaactatctt tataattaca atctatcaaa attctaaata attaacaatt
    10501 tattttaaat tatgtattat atgtagtgta tacttttttt attatcaata attcaaaaaa
    10561 gagggaattc aatggaaaca acattttatt cgttagctac aaaaactaat tttaataagg
    10621 ctttaaacca tttctacagg gttaatgatg tggtggatga gattgaatca ggtagaaatt
    10681 ttgcagatgc agtcaatgtt atgctggctg aaggggattt aactgttgac caggtattac
    10741 caatgatcgg tgcaataata aaagataaat atcagtattc ctatgattca tataatgtac
    10801 cggtaacaat taatgatttc agcaaaatag ttgaagaaac atcaaaatgg acagcacttg
    10861 atataattct tgtgtatttt accgttaaca gcgaaccttt tttaataaat cctaaaaata
    10921 tgattcattg ggaacgtgtc agggatattg ttagggacca gcttatggtt atttacgtta
    10981 aaaaaaagaa agaaccaaat ccaaaagtgg aaaatgatgc tataaatgca attgaagaga
    11041 tgatggctgg caaggatgtt tttatcaata aagagttcat agatgaaaca gttgcggtcc
    11101 gggaggccaa aaagcaaatt gctccagctg aagcggctgc agcaggaaag aaaaatatga
    11161 ctcccaaata tgcggtgaca gttaccaatg agttatttca caacgggaac gtggaagcat
    11221 ggaagcgtat tattgaaagc tttaaagtat cgcatcctga tttagacgtc ttgatattcc
    11281 atgaaggtga aaagattaat gatatcaact ccctctttaa atggggaaag gttaaacacg
    11341 gtgatccaat cttatttcag gtggttggtg aaaatatcag aaatgtatca aaattacaga
    11401 aatatctgtt tgaaggtgca tcacccaggt ttgaacaatt tttaaagata gcagtaggaa
    11461 aagttttaaa tctattttaa tgtaatgtgc gatagaacat tttaaaagag agggttgtat
    11521 ggaaaatata gtttttttta ataaagattt taccggtgtt gataaaaagc aatataaaga
    11581 attgggaatt agaggtcgtc gtgctgctga tttagcaaag atgggtttgc caatagctcc
    11641 tggatttatt gttgatgcag agttaacaat gaaattgcca aaggtgaatg ttaaacagct
    11701 tattaaaagc tatgttgata aaattgaaaa ggacatgaaa aaagggtatg gtgatactga
    11761 aaaaccactt cttcttaaag ttgtgttaag ttcagatctt aatgttccat tttttccatc
    11821 aatacataat gttggtttaa atgatgcaat tgttactgct tttgcaaaat ttaccggtga
    11881 agattttgca tggggtgagt ataggttttt actgagaagc atagcctcaa agatttatgg
    11941 tattgatgaa aaagaactgg atgaactgga agggaagctc cccaaaaacg caaaagcaaa
    12001 agaatataaa gaagtagtta caaaatataa aaagtttttg ggggataagt ttagtgagga
    12061 tgtatatgat cagttaggcc ttattttgaa aggggctgct gcacggtact gtgattcgga
    12121 tattgatgtt gatgattcac tggcaataac tattcaggca atggtgtatg gcaactttgg
    12181 ccagaattcc tttgcaggaa attattttac ccgcaatatt attaccggtg atgcacttat
    12241 tcagggcgat tttttgcaga atgagtttga tgttgaccgt ggcactccaa aagatattgc
    12301 aaagattgat aaaaaatact atgataaatt agttgaaatt gcaaaaaaag ttgaggataa
    12361 ctttaaagag atacggaata tcaagtttac tattgaagag ggtaattttt ggctcattga
    12421 acagcgcgag gttgatgaaa aatcaactca ggcacatata aagacactcc ttgatcttaa
    12481 tacacgtaag gttgtaagtt caaatttttt aattgaatct attaagccca gtcaacttaa
    12541 tgaactttta caccctgtta ttgatccccg aacaacaaag aatgtaaagt ctataaaagg
    12601 tggaattgcc gggtcaaccg gtgcagcaat aggacgagta ttcttttcaa ccgacaagct
    12661 gctggaagaa tacaaacgtt cggtgatgca tggtggggat accaacctta tacttgttat
    12721 gcctgcaact tttgcagaag atgtgaaagc tatcgaggta gcccgtgggg ttattacatc
    12781 agaaggtggc ttttcttcac atgctccggt tgtggcacga agcttaggta aggttgcaat
    12841 ggtgcagcct gagataaaga ttagagggaa ttcgtttaca ctggcaggga aaaccgttaa
    12901 agaaggtgat tatgtttcga ttaatgtccc ttattatgaa ccgcctacca tttatcttga
    12961 taaagttggc ttaattgaac ctgattttga aaagaatggt ttaattgact ttttaaagat
    13021 agtccaaagc ttcattaaag actttgatgt acgtgcaaat gctgatcagc ctcgtgatgc
    13081 aaaagttgca aagatgtttt ttgcagatgg gataggatta tgccgtactg aacatatgtt
    13141 tttcaatgaa aaacgaatca tgaaatttcg tgaaatgata ttatctgaaa ctgaagtcga
    13201 gcgaagaaag gcattggatg agttgaaacc aatgcagaga agcgattttt atgaattatt
    13261 taaaattatg cagggacatc cagttactat acggctttta gatgctcctt tacatgagtt
    13321 tctgccaaga actgatgata gcatgaaaga cttcatacaa tatatgaagt cgcgtaaaaa
    13381 aggcatgtct gatgctgaaa ttagaatcag gtgtgaagag ctatctgaaa tgaatccaat
    13441 gctgggacat aggggctgtc gtgtagcaat tacgtatcct gaaatttatg aaatgcagtg
    13501 cagagcaatt tttgaagcgg catgcatgtt aaagaaagaa ggaattgatg ttgttcccga
    13561 aattatgata cccattgtca tgagcgaaac cgaaattaag tttattaaaa atggcaaaag
    13621 aattgaaggg aaaacagtta aaggtatacg ggatataaaa gatgaggtag ttgaagagta
    13681 cggcatcgat gatttggaat atgaagttgg cacaatgatt gaattgcctg ctgctgctgt
    13741 tggagcagga cgaatagctc aatatgctga gttttttagt tttggtacca atgatcttac
    13801 tcaaactacc tatggattat cacgcgatga tataaattcg tttttcccaa gctattcgtt
    13861 gtatgacctg attcccaata atccgtttaa ggtattgggg cgtgaagtta aagagcttat
    13921 tgaaatggct tcaactcgtg gaagcctaac tcgtccagat atcaccatgg ggctttgcgg
    13981 tgaacatgga gctgacccgg ataatattga attttgtatg aatgttggtt tgaactatgt
    14041 ttcctgctca ccgtatgcaa taccattgtc aaagttagcg atagctcagt tgaacattaa
    14101 aaacgcaaag agtaagtaag ttatcgatag ttactgtaca gtaactatcg ctcatactaa
    14161 aataaaaagg ctgctttaca taagcggcct ttttattgct tacagatttt agaatgtggg
    14221 ttcttttata atgttatttt ggaaaagtgc atggaaaggc aatcaaataa aagaagctga
    14281 tttgctgtaa tggtgcctaa tcctgttaca agtttaattg cttcaagggc ttgaagtgtg
    14341 cccataatac aaggtgtaac gccagctact ggaattggct tcttgatgct tttctttggt
    14401 agaaaacacg ataagcatgg agtttggcct ggaagtatga ctgtaagctg gccgctgaat
    14461 ccttccactc cggcgtggat taatggcttg ttttgttgta cgcatgcctt gttgagaata
    14521 tggcgtgttt caaagttatc caggcagtcc agaatgatgt gtgctttttc tatgagtgta
    14581 taatggtgtc ataatcgcag agtgtgattg taccaaaccc ggctactgca cagtataatg
    14641 atgcggtact tgcaagccca ccagctcctg caataaagat gtgggttttc tttatttttt
    14701 cttgagattc tttgccccag cctttgataa tgagctgacg ctgatagagt tcaagttctt
    14761 caggtgttag cataacgaat ccttatgata tggattaaat aattgttaat gttttacatt
    14821 ctaaaattga tttgttactt aacgaaagta ttcaatataa gaaaagtcaa ttatatttta
    14881 aattctattt gaagaatcca catatcaatg acatggaaaa taaggtaa
//
LOCUS       DAOSOX010000039        14911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4037_length_14911_cov_8.770732, whole genome shotgun sequence.
ACCESSION   DAOSOX010000039 DAOSOX010000000
VERSION     DAOSOX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4037_length_14911_cov_8.770732"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..2353
                     /locus_tag="PLX22_04505"
     CDS             191..2353
                     /locus_tag="PLX22_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT19189.1"
                     /translation="MIKKFLKKILLYQIIVLPGLTVFTIFFIYAIVFNMSFSYMGNTS
                     KNVVDIIISQYILLIIISFIKILAAYLCIGLSLSIFFYYGITALTSLFNASLGNISKS
                     FLTAALTFIIAFLQLCKSIVMYPQLYLDTIFNKNIIYKNFQILLTNNFSPDFFMALQC
                     SIVVPFIAAFVFVVYRQLFNHMKIYFQQMASLRNKKKQMIVACVIVTIVTISLLPITI
                     AHNKTTTKPNIIILASDALRPDHFSGYGYYRKTTPTIDTLIQQGANFHNVYTVVPRTF
                     PAWVSIITSQLPMKHHISHMFPMAVTRKQNFITLPKILSHHGYYTSVVGDFAADIFPR
                     IDLGYETVKAPTFNMRVMIQQIILKNHTFLLPFLTNKAGFYCFPEIKEFAEFADPEFV
                     VQDIKTEISKSIKTKKPFFITSFFSISHFPYPACYPYYNLFTDRHYSGPFKYSKNRVV
                     SLNSEGEAHEYSPSENEVEYIRALYDGCVYAFDEAVGKVIQYCKDKNIYDNTIIIILS
                     DHGENLYEYDYGMGHGEHLRGKYSMVIPCIFAGPGIPYQHHRAVRSAIDIAPTILDLL
                     GIQKPESFDGTSLLEKHKRNFDAYCETGIWFDNTGNFFFQKKRIMYPDITGISTIDFH
                     YHDEITITPYYEYCITIAKHRCIIANNYKLIYMPTHYGIEYELYDLSNDSDERNNIFT
                     TSLQSEMLKKKFFSLFKDDKTFTLYNEYLLPVIDKPIF"
     gene            complement(2466..2867)
                     /locus_tag="PLX22_04510"
     CDS             complement(2466..2867)
                     /locus_tag="PLX22_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOT19190.1"
                     /translation="MYEKNNIDAGDSFKVQQEIDKYRAIYYAGASGDFNPIHIDAEFG
                     KMVGLGGAILQGLCTLGFTADAVTTWIGDPGRLKKLKCRFSLPVKMGDIITIEGLVTE
                     VRGDSGTVEIKVINQNGSDVLTNVQAEVVAK"
     gene            complement(2879..3316)
                     /locus_tag="PLX22_04515"
     CDS             complement(2879..3316)
                     /locus_tag="PLX22_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOT19191.1"
                     /translation="MTVDKKFIGKTWPTVIYEVGKEKIKEFAQAIKSNNPYYFNDEFA
                     LQSKYKTIVAPPTFAVVFGAKLIEPIFMDNELSLNIAMLVHGEQEFEFYTMVKAGDIL
                     YSNARIIGIENKEKLDVVTIEIMTKNQYNQDVCKGIYTFVVRK"
     gene            complement(3331..4500)
                     /locus_tag="PLX22_04520"
     CDS             complement(3331..4500)
                     /locus_tag="PLX22_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT19192.1"
                     /translation="MNYELNDIQKSIKENFKKFCENEIKPNASILDAGDEKAVELLKN
                     NIKKLGQLGYMGMIHEEAFGGTDQDLINFAIAGEEVSKACAATFLSAGASCGLCGMPI
                     RLFGNDKQKEKYLPGIISGDVIGSFGLTEPQAGSDATAIATTAKRKGAKWILNGTKTF
                     ITNAPIADVCLIMAYNDKEAGPGAGVTAFLVDTHTPGFSIGKPFDKLGYRGSPTSEIF
                     LEDCEVSDDAVLGELGKGFIQAMQTLEYGRIGMATVSLGVAVACMDEAVKYASVRKAF
                     GKPINRYQEISFKLADMMILTDLSRLLIYKAAWAKQMNDPEAAVLASCAKLFASEAAT
                     QIASWALQVHGGYGYIKEFPVERLYRDAKLGEIGEGTSEIQRILIARDILQKYGS"
     gene            complement(4526..5674)
                     /locus_tag="PLX22_04525"
     CDS             complement(4526..5674)
                     /locus_tag="PLX22_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT19193.1"
                     /translation="MEFSFSEEQLLFRDTIYKYAKNEIVPLCEEADLKSEFSFEIWQK
                     LARMGLLGLPFPEEYNGQGADVVTCCLASEALGHAGVDSGHLLALGAHTYLCTDTLFK
                     HGNEHQKKTYVHKLANGEWIGCMGLTEPGAGSDAAALQTTAVKKGDRWILNGSKTFIT
                     NAPVADVCVVYATVDKSKKHAGITAFIVEKNFKGFSTGKPFHKLGVRASTTSEIFLED
                     CEIPEDNVLGNIGEGFMYAHQTLEWDRSALLAPFVGAMQFMIEQCARYATERVQFDKP
                     IKSFQAIQHKLADMKMVMEAARLAVYRVASLKDAGYPINHLQASIAKAFVGDWGFKVA
                     SDAVQIFGGYGYIHDYPVERMIRDAKLAQIGGGTSEIQRLIISRLLTL"
     gene            complement(5664..6290)
                     /locus_tag="PLX22_04530"
     CDS             complement(5664..6290)
                     /locus_tag="PLX22_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19194.1"
                     /translation="MRVKQERNSIYDTRRLQLTAAAYNVVSKKGYYNFTIKDIADEAG
                     MSAGLVHYYFKNKQDLLLNLLRQINTNIRLALQNDLANIEDPVDKLSVFILRACNLII
                     EEKNYFYVLLDFWTQLNHNERMRKAIQKLYQSYRKVCSDILNEGIIKGKIKQIDVIYT
                     ATMIVSMVQGLIIQYVIEPEAFDYSYYVNKIKNQIIETIISKGVNNGI"
     gene            complement(6345..7136)
                     /locus_tag="PLX22_04535"
     CDS             complement(6345..7136)
                     /locus_tag="PLX22_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19195.1"
                     /translation="MSLIEVNNDMRVIMDREAFFVMLLAGTDKIRSGYGNGDSLLIDS
                     EHSIFAIADATERFPNASRILLSKIMGGLASIPDTSEQWRAYINAIYRQQEYHLKTTL
                     SLIALKQSHNGCEAFIVHGGDSSLKIFNRLNKKLLYKSTANMNFAGRSTSIDTIDSIN
                     LESNTRIIIHSDGFNDIMKIGAIDFDDIIAAPLFDCISLILSQVNAKNFNNEYDDVGL
                     LIVDVPEIFNVISGGILMGGTTHCEERCLQNNLTICDYSSLIKTF"
     gene            complement(7140..7604)
                     /locus_tag="PLX22_04540"
     CDS             complement(7140..7604)
                     /locus_tag="PLX22_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOT19196.1"
                     /translation="MENLEHIIIESGKAVQPFHYSVGMYGSKFFHELKNNRKFVGVRC
                     PKCKKVYVPPRQVCGECFMEMKDFIEVGPKGIISTFTILRFAFIDPETGLQKPVPYGY
                     GFIKLDGADTLFQHFIDVTEESKVKIGARVEPVFSDEKKGTIKDILYFRIID"
     gene            complement(7615..8778)
                     /locus_tag="PLX22_04545"
     CDS             complement(7615..8778)
                     /locus_tag="PLX22_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT19197.1"
                     /translation="MAKRRVAIVGTGQTNHKSHRPDVNGQELIHEAVVRALQDAELSI
                     NDIDAIIIGNMDHFEGINYVDCWSIDGSGGYHKPIMKLTTGGTTGSTLAIGGYHLVAS
                     GMFDKVLVIGWEKNSESDTTGAITTAFDPIWDRLVFAGAISGLAVEAQSYMAKYGATD
                     RDGARVSVRDRKHACNNPYAQLRKEVTLEEVLASPMLADPIHLLDVCPRTDGACAVVM
                     ASEDCAETITSTPDWIWATANRHTYSYLGDVNWGELDSIADASKELFKKSGISEPRKQ
                     IDVIEMYQPYSLAGIIWIEDMGIIPRGKGAEYIWSGATDMGGELPINPSGGVIACNPI
                     GATGLIRCAEAAMQVMGKAEGRQVPDVNFAVSTGFGGCMWTDMILHGKKKPNL"
     gene            complement(8788..9927)
                     /locus_tag="PLX22_04550"
     CDS             complement(8788..9927)
                     /locus_tag="PLX22_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT19198.1"
                     /translation="MDAAIIGIGMTTIEKNKIACNYADLAWEAVNKALADASITIDDI
                     DNVITTSSDFWDGRTISCMAVGDACAVRHKNCSAVEGDGTFGAAYGLSRTLSGDYKTT
                     LVTAHSKGSESISSIITNAAFDPIYERSLGLDMISASAIQAKQYMHVTGTTPEQLALV
                     SCKNHKNGKDNPYAQLPMDITVNDVLNSEMIAEPLHKLDCSPVSDGCCAIIIANAEFV
                     KKVKAKPVWIKGVAFCSDAYFMGDRDLTKTIALENAAKKAYMMAGITNPENEIDIAEI
                     YDAFTYQELMWIEGLGLAEYGNAGKLLEKGIFNKNGKLPVNPSGGLLSGHPVIAAGLY
                     RIAEIVLQIRGEAGARQVNAKRGIAHGVNGICGQSHCVWVLDKEK"
     gene            complement(9938..10399)
                     /locus_tag="PLX22_04555"
     CDS             complement(9938..10399)
                     /locus_tag="PLX22_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOT19199.1"
                     /translation="MGNIFHGIEPSVYKSQINVPYQWWAGETASKYFIALRDEKKILG
                     KQCPQCGKVYCPPKKTCPSCFTETNAWKEVGPKGKLISYTIVLRQLAALQKKVPVAFG
                     LICIDGADNAMLHYIGDVDVNNITIGMEVEAVFADDRNATINAISHFKPLT"
     gene            complement(10412..11179)
                     /locus_tag="PLX22_04560"
     CDS             complement(10412..11179)
                     /locus_tag="PLX22_04560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT19200.1"
                     /translation="MDYNAMELMICVAARNLEDGATVVVGTGAPCAAAMLSQKIYSPN
                     LCILFEAGGVAPILPTMPISVGDSRTFHKAIMASSMPEVMETCQRGMVDYTFLGGAQI
                     DMYGNINSTMIGDDYQKPKVRFPGSGGANDLASLCWRTMMMTVQDARRFTDKLDFITS
                     PGYLKGGRSRYDAGLPEGTGPYRIITNMAVMGFDDETKWMMVLSINPGYSRQDVQDNC
                     GFELKWAKKIDDTKPPADEELRILREEVDPHHYIIGR"
     gene            complement(11191..12207)
                     /locus_tag="PLX22_04565"
     CDS             complement(11191..12207)
                     /locus_tag="PLX22_04565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT19201.1"
                     /translation="MALGTRDNDTEGVMLSGKNELFTDPDPDKARAFFRNKSRSMTPK
                     LAQIKDVVRDMIKDGDYIAIGGFGANRIPTAIVHEIVRQHKKHLGFAGHTSTHDFQVF
                     IAGKCIDRCDVAYIIGLEARGLSPNARRAVESGTIKLTEWTNASLSWRLKAAAMGISF
                     IPTRNILGTDTFKHSAAKTITCPFTGKKYAAMPALFPDFAAIHVHEADIYGNCRVYGA
                     SVSDFDLAKASKRVVITTERIIPHDEIRREPEKTVIPYWCVDAIIEVPYGSYPGNMPY
                     EYFSDEEHLKTWLKVERDPEELEIFLQEQIYGVNDFYEYLNLNGGIEKMKKLRAIEYL
                     INNK"
     gene            complement(12285..14111)
                     /locus_tag="PLX22_04570"
     CDS             complement(12285..14111)
                     /locus_tag="PLX22_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT19202.1"
                     /translation="MEEKTINQVFRNRVQKYGSRIALEKKLNGKWESVSWEDYYKRAA
                     YVGLALNDMGLQKGDRVSLLSENRLEWLYADMGVLGAGGCIVPIYPTLIDDEVEYIVS
                     HSDSKFLIVENNAQLQKGLYTAKRYPGLKKIIVIDTTEVNVNPMIMDYNELLDKGKVL
                     YNKNPEAFASLADTIAQDDLATIVYTSGTTGVPKGAMITHKNIMAVIKALDTIEPHYA
                     YDSDQTVPFLPLSHVFERVAGHFYGMYVGITASYAESLDTLIQDIQEKRPTVVLAVPR
                     VLEKVYQQIISQVQEQSPFKQKVFYWGQKVGSKISELREQKKNPGFNLSLKYKIAYAI
                     IFKKLQNALGGRVRWMTASGAPTAREIILFFNSAGIMVIEGYGMTECCAPATMSNIAD
                     YRIGTVGRPLPGVDIKIADDGEILIKGDNVFKGYWKMPEETKDSFTQDGYFMSGDIGL
                     FDDAGFLMITDRKKDLIITSGGKNVAPQKIENLIKSDPLFTEAIVIGDKRKYLIALVN
                     ISQEQAERIAKEKGINVNSLHDLFNNPRFQTIVEEHIEKCNQKLARYETVKKVGIIKN
                     EFSKETGELTATLKVKRKAVQQKYQSIIDSLYEEDAKTIQIY"
     gene            complement(14129..>14911)
                     /locus_tag="PLX22_04575"
     CDS             complement(14129..>14911)
                     /locus_tag="PLX22_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT19203.1"
                     /translation="IMNDKCYYQIEKENVAVVYFNNPPINALDTETMESFEEIMRILI
                     TDDKVHVIVLTGKGSTFIVGADVNEVAKVNTFDKGVEITTKAQAIVSLCELSPKPVIA
                     MINGLCLGGGMEIALSCHIRIASNTAQMGLPEIMLGIIPAFGGTQRSPRLLGKAKALE
                     FMLTGMFINMQEALQIGLVNRVYAPDELIEESLKFASSIAKKGQIAVRSIVEAVMKGS
                     SMNLEDGLRLESTLFGRLAESHDKKEGIDAFLNKRKPIFKHC"
BASE COUNT         4453 a         3264 c         2185 g         5009 t
ORIGIN      
        1 atcaatattt aattatttac aattatacct tttgcttgaa atgtgctata tactcaataa
       61 cagagtatat ataaactatt atgaaaattt tgtaaatgat gcaagttcaa taattttatt
      121 acagtttagt actaacacac tgatatttta aacatgaaat tatatttcta ctttttttag
      181 aggaatagtg atgataaaaa agtttttaaa aaaaatattg ttataccaaa taattgtttt
      241 accaggctta acagttttta ctattttttt tatctatgcc attgttttta atatgtcatt
      301 ttcatatatg ggcaatacca gcaaaaatgt tgtagatatc attatttcgc aatatatatt
      361 gttaatcatc atatcgttta taaaaatttt agcagcatat ttatgtattg gattatcttt
      421 aagtatattt ttttactatg gaataacagc attaacatca ctgtttaacg catcactcgg
      481 caatatttca aaaagttttt taactgcggc attgacattt attattgcat ttttgcaatt
      541 atgcaaaagc attgtcatgt atccgcaatt atatctggat accatcttca ataaaaatat
      601 aatctacaaa aattttcaaa tacttttaac caataacttt tctcctgatt tttttatggc
      661 gttgcaatgc agtattgttg tgccattcat tgctgctttt gtattcgttg tgtatagaca
      721 attatttaat catatgaaaa tatattttca gcagatggca tcgttacgca ataaaaaaaa
      781 gcaaatgatt gttgcatgtg tgatagttac tattgtaaca atatctttat taccaataac
      841 tatcgcccat aataaaacca ccaccaaacc aaacattatt attttagcca gcgatgcatt
      901 gcgccccgac cattttagcg ggtatggtta ttatcgtaaa acaacaccca ctatcgacac
      961 gctaatacaa cagggagcaa attttcacaa tgtttacact gttgtaccgc gtacattccc
     1021 ggcatgggtt agtattataa catcacagct acccatgaaa catcatatta gccacatgtt
     1081 tccaatggcg gttacccgaa aacaaaattt tataacattg ccaaaaatat tgtcacacca
     1141 tggttactat acatcggtgg ttggcgactt tgctgctgat attttcccgc gtattgattt
     1201 gggctacgaa acagtgaaag caccaacatt taatatgcgt gttatgatac aacagatcat
     1261 cttaaaaaat catacgttcc ttttgccatt tttaaccaat aaagctggtt tttattgttt
     1321 tccagagata aaagaatttg ccgaatttgc tgaccccgaa tttgttgttc aggatataaa
     1381 aactgaaata agtaaaagta taaaaacaaa aaagccattt tttataacat catttttttc
     1441 aatatcgcat tttccctacc ctgcatgcta tccctactat aatttattta ccgacaggca
     1501 ctatagtggt ccatttaaat attctaaaaa cagggttgtg tcacttaatt ccgaaggtga
     1561 agcacatgaa tactctcctt ctgagaacga agttgaatat attagagcat tatatgatgg
     1621 ttgtgtctat gcgtttgatg aagctgttgg aaaggttata cagtattgta aagataaaaa
     1681 tatatatgac aacaccatca tcataatcct ttcggatcat ggtgaaaatc tttacgaata
     1741 tgactacggc atgggacatg gcgagcatct gcgtggtaag tatagcatgg taatcccctg
     1801 tatctttgca ggtccgggta taccatacca acatcaccgt gctgtacgaa gtgctataga
     1861 tatagcaccc actatccttg atctactggg tattcaaaaa ccagaatcat ttgatggcac
     1921 atccctttta gaaaaacaca aacgcaattt tgatgcatac tgtgaaacag gtatatggtt
     1981 tgataatacc ggtaacttct tttttcagaa aaagcgtata atgtacccgg atattaccgg
     2041 tatctcaaca attgattttc actaccatga cgagattacc attactccat attatgaata
     2101 ttgtattact attgcaaagc atcgatgcat aattgcaaac aattataaat tgatatatat
     2161 gccaacccat tatggcattg aatatgaact gtatgattta tcaaatgata gtgatgaacg
     2221 aaataatatt tttacaactt cactacaatc tgaaatgttg aaaaaaaagt tcttttcatt
     2281 atttaaggat gacaaaacct ttacactata taatgaatat cttttacctg ttattgataa
     2341 gcctattttc taagaatgaa atgatgcatt gcgatactat tttcaaaaga ttgtgaaacc
     2401 accttatcaa tagttttcct ggattaaaag aatagtttgc aaagatagca gcaataccat
     2461 acaatttatt ttgccactac ctcggcctgt acatttgtta aaacatcact cccattttga
     2521 ttaataactt ttatttccac tgttccagag tccccacgca cttctgttac caatccttct
     2581 atggttatga tatcacccat ttttaccggt aacgaaaatc ggcattttaa tttttttaat
     2641 ctgccagggt caccaatcca tgtggtaact gcatcagcag taaaacccag agtgcacagc
     2701 ccctgcaata ttgcaccacc caacccaacc attttcccaa attcagcgtc aatatgtatt
     2761 ggattaaaat ctcctgatgc accagcatag taaatagctc ggtatttatc aatctcctgt
     2821 tgtactttaa aagagtctcc agcatctata ttgttttttt catacattgt tttcatcctt
     2881 atttcctcac tacaaaagta tatattcctt tacatacatc ctgattgtac tgattttttg
     2941 tcatgatttc aattgtcact acgtctaact tttctttatt ttcaatacca ataatcctgg
     3001 cattggaata taaaatatca ccagctttta ccatggtata aaattcaaat tcctgctcac
     3061 catgcacaag catagctatg tttaaactaa gctcattatc cataaatatt ggttctatca
     3121 gttttgctcc aaacactaca gcaaacgttg gtggtgctac tatcgttttg tatttagatt
     3181 gtaaagcaaa ttcgtcattg aaataataag gattgttact ctttattgct tgtgcaaact
     3241 cttttatttt ttctttaccc acttcatata tcactgttgg ccatgtttta ccaataaact
     3301 ttttatcaac agtcatagat atctcctcca ttagcttcca tatttttgta aaatatctcg
     3361 tgctatgagt atcctctgta tctcagaagt accttcacca atttcaccca attttgcatc
     3421 ccggtataac cgttccacag ggaattcctt aatatagcca tacccaccat gaacttgcag
     3481 tgcccagctt gctatttgtg ttgctgcttc actggcaaac agttttgcac atgaagccaa
     3541 tactgctgcc tcaggatcat tcatttgctt ggcccaggca gctttatata tcagtaatcg
     3601 agataaatca gttaatatca tcatgtccgc cagtttaaat gatatttcct gataacggtt
     3661 tattggttta ccaaatgctt ttcgcacaga tgcatacttt accgcctcat ccatacaggc
     3721 aacagccact ccaagagaaa ctgtcgccat acctattctt ccatattcaa gagtttgcat
     3781 agcctgaata aatcctttac ccaattcacc gaggacagcg tcatccgaaa cttcacaatc
     3841 ctctaaaaat atctcagatg tcggcgaacc tctataccct aatttgtcaa atggctttcc
     3901 aatagaaaat cccggtgtat gcgtatcaac caaaaaagca gtaacccccg caccaggacc
     3961 ggcttcttta tcattataag ccattataag acatacatca gcaatggggg cattggttat
     4021 aaatgtttta gtaccattta aaatccattt agcacctttc ctttttgccg ttgtagctat
     4081 agctgttgca tctgaaccgg cttgcggctc ggttaatcca aagctcccaa ttacatctcc
     4141 cgatataata ccgggtaaat atttttcttt ttgcttatca ttgccaaata gccgaatagg
     4201 cataccacat agtccacaac ttgcaccggc tgataaaaaa gttgcagcac atgcttttga
     4261 aacttcttca ccagctattg caaaattaat aaggtcttga tcagttccgc caaatgcttc
     4321 ttcatgtatc atgcccatat aaccaagctg acctaatttt ttaatattat ttttcaataa
     4381 ctcaacagct ttttcatcgc ctgcatccag gatactggca ttgggtttta tttcattttc
     4441 acaaaatttt ttaaaatttt cttttattga tttttgtata tcatttaact catagttcat
     4501 ataatagcct cctgacctaa ctatattaca atgttaacag acgtgatatt attagccgct
     4561 gtatttcaga tgttcccccg cctatttgag ctaattttgc atcccgtatc atcctttcaa
     4621 ccggataatc atgaatatag ccatacccac caaaaatttg tacagcatca gatgctactt
     4681 taaagcccca atcaccaaca aatgctttgg cgatagaagc ctggagatga tttattggat
     4741 atcctgcatc ctttaatgaa gcaactctgt ataccgctaa ccgtgctgct tccataacca
     4801 ttttcatatc agctaattta tgctgtattg cctgaaagct ttttataggt ttatcaaatt
     4861 gaactcgttc agttgcatat cgtgcacatt gctctatcat aaactgcatt gcaccaacaa
     4921 agggagccag taaagcgctt ctatcccatt ccagcgtttg gtgagcatac ataaatcctt
     4981 cgccaatatt acccaatacg ttatcttcag ggatttcaca atcttctaaa aatatttctg
     5041 aagtagtaga tgctctgacc cccagtttat gaaaaggttt cccggtggaa aatcctttaa
     5101 aatttttttc cacaatgaat gccgttatgc cagcgtgttt ttttgattta tcaacagtcg
     5161 catacaccac acaaacatca gcaacaggtg cattggtaat aaatgttttt gaaccattta
     5221 atatccatct atcgcctttt tttactgccg ttgtttgtaa cgctgcagca tcagagccag
     5281 cacctggttc tgtcaatccc atgcatccta tccattcccc atttgctaat ttatgtacat
     5341 atgttttttt ttgatgctca tttccatgtt taaataatgt atcagtacat aaataggtat
     5401 gggctcctaa agccagaaga tggccgctat caacgcctgc atgacctaaa gcctctgatg
     5461 ccagacaaca tgtaacaaca tcagcaccct gtccgttata ctcttcaggg aatggtaatc
     5521 ccaataatcc catcctagct aatttttgcc agatttcaaa tgaaaactct gatttcaaat
     5581 cagcttcttc acatagtggt actatctcat tttttgcata tttatatatt gtgtccctga
     5641 ataaaagctg ttcctcagaa aaactaaatt ccattgttta ccccctttga aattattgtt
     5701 tcaataattt gattttttat tttatttaca tagtatgaat aatcaaaagc ttcaggttct
     5761 ataacatact gaatgattaa accctgcacc atggaaacaa tcatcgtagc tgtatagata
     5821 acatcaattt gcttaatctt gcccttaatt atcccttcat tgagtatatc cgagcatact
     5881 ttccggtaac tttgatataa cttttgtatt gctttcctca tacgttcatt atggttgagc
     5941 tgtgtccaaa aatctaaaag tacatagaaa taattttttt cttcaattat gagattacat
     6001 gccctgagta taaaaacact caatttatca actggatctt cgatattagc cagatcattt
     6061 tgcaatgcca atcgtatatt ggtatttatc tgacgcagaa gatttagcag caaatcctgt
     6121 ttatttttaa aataataatg aaccaaccct gcagacatcc cggcttcatc tgctatatct
     6181 ttaatagtaa aattatagta tcctttttta ctcacgacat tatatgctgc agcagtaagc
     6241 tgtaaacgcc gtgtatcata tatgctgttt cgttcctgct taactctcat cagtatcctc
     6301 tacattctga ttgaatgacc aatcaattat aaactatacc atattcaaaa agtttttata
     6361 agagaactat aatcacatat ggttaaatta ttctgcaaac atcgttcctc acaatgagtt
     6421 gttccaccca ttagaatacc accagatata acattaaata tttcaggtac atcaactatc
     6481 aataatccca catcatcata ttcattgtta aaatttttag catttacctg gcttaaaatt
     6541 aatgatatac aatcaaatag tggagcagca ataatatcat caaaatcaat agcgccaatc
     6601 ttcatgatat cattaaatcc atcactatgt ataattattc gtgtgtttga ttctaaatta
     6661 atggaatcaa tggtatcaat agatgtactt ctccctgcaa aattcatatt ggctgttgat
     6721 ttatataaaa gttttttatt taagcgatta aatatcttta aagaactatc gccaccatgt
     6781 actataaatg cttcgcatcc attatgtgac tgttttagtg ctatcaaact caatgtggtt
     6841 tttaaatggt attcctgttg tcggtatatt gcattgatat aggcacgcca ctgttcagaa
     6901 gtatcaggaa tgctggctaa acctcccata atttttgaaa gcaatatgcg tgatgcattg
     6961 ggaaatcgtt cggttgcatc agcaatagca aatatactat gctcactatc aattaacaaa
     7021 ctatcgccat taccgtatcc agatctaatc ttatcggtac cggccaaaag cataacaaaa
     7081 aaagcttccc tgtccattat tacccgcata tcattattta cttctatcaa actcatatgt
     7141 taatcaatga tcctgaaata taatatatct ttaatggtgc ctttcttttc atctgaaaaa
     7201 acaggttcta cacgtgcacc aatctttact ttagattctt ctgttacatc aataaaatgc
     7261 tggaatagcg tgtcagcacc atccagctta ataaatccat agccatacgg tactggtttt
     7321 tgcagcccgg tttctggatc tataaaagca aaacgcaaga ttgtaaatgt actgattatt
     7381 ccttttggtc ctacctctat aaaatccttc atttccataa aacattcacc acacacctga
     7441 cgcgggggaa catatacttt tttgcattta ggacatcgaa ctccaacaaa ttttctgtta
     7501 tttttaagtt catgaaagaa cttactgccg tacataccca ctgaataatg aaaaggttgc
     7561 acagctttcc cggattctat aattatatgc tcaaggtttt ccatactttc tctcctaaag
     7621 attgggtttc tttttaccat gtagtatcat atctgtccac atgcaaccac caaagccagt
     7681 gctaacagca aagttaacat caggaacctg ccttccttca gcctttccca tgacctgcat
     7741 ggcagcttct gcacagcgta ttaatcctgt agcgccaata ggattacatg caataacacc
     7801 acctgacgga ttgatgggga gttccccacc catatctgtt gctccgctcc atatatattc
     7861 agcacctttt cctcgtggga taatccccat atcttcaatc catataattc ctgccagtga
     7921 atacggctga tacatttcaa tgacatcaat ttgttttctt ggttcactga taccagattt
     7981 tttaaaaagc tcttttgaag catcagctat gctatccagc tcgccccaat tgacatctcc
     8041 taaatatgaa tacgtatgtc gatttgccgt tgcccaaatc cagtcaggtg ttgatgttat
     8101 tgtttctgca caatcttcag atgccataac aactgcacac gcaccatcag ttcgcggaca
     8161 cacatctaaa agatgtattg gatctgccag catgggcgat gctaatacct cctctaaggt
     8221 aacctccttt cgcaactggg catatggatt attgcacgca tgctttcggt cacgaacaga
     8281 aacacgcgca ccatccctgt cagtggcacc atactttgcc atgtaacttt gcgcttcaac
     8341 ggccaatccg gagatagctc ctgcaaatac aagcctgtcc catataggat caaatgctgt
     8401 ggtaatagca ccggtagtat ctgattcaga atttttttcc cagcctataa caagaacttt
     8461 atcaaacatt cctgatgcaa caagatgata tccaccaatt gcaagtgtac tccccgtagt
     8521 cccaccagtt gtaagtttca taataggctt atgataacca cctgaaccat ctatactcca
     8581 gcaatctaca tagttaatac cctcaaagtg atccatattc ccaataataa tggcatcgat
     8641 atcattaatg gacagttctg catcctgtaa tgctctaact accgcttcat gtattagttc
     8701 ctgtccatta acatcaggtc tatgactttt atgattagtt tggcctgtgc caacaattgc
     8761 aactctgcgc ttagccatac catcctccta tttttcttta tccagaaccc atacgcagtg
     8821 tgattggcca catataccgt taacaccatg ggcaatgcca cgtttagcat ttacctgccg
     8881 tgcacctgct tctccgcgaa tctgcaaaac aatctctgca attctataca aacctgctgc
     8941 aatgactgga tgtccactta acaatccacc cgatggatta accggtaatt taccattttt
     9001 attaaaaatt cctttttcta aaagtttgcc agcattccca tattctgcca acccaagccc
     9061 ttcaatccac atcaactcct ggtaggtaaa cgcatcgtat atttcagcaa tatcaatttc
     9121 attttctgga ttggttatcc ctgccatcat atatgctttt ttagcagcat tttccagtgc
     9181 gatagttttt gttaaatctc tgtcgcccat aaaatacgca tcactgcaaa aagccactcc
     9241 tttaatccac accggtttag cttttacttt cttaacaaac tctgcatttg ctatgataat
     9301 ggcacaacaa ccatctgaaa ccggagaaca atctaacttg tgtaatggtt cagcaatcat
     9361 ttcagaattg agtacatcat ttactgtaat atccattggc agttgcgcgt aaggattatc
     9421 ctttccattt ttgtgatttt tacaggaaac aagggctaat tgttcagggg ttgttcctgt
     9481 aacatgcata tactgctttg cctgtattgc actggcactt atcatgtcca gtcctaagct
     9541 tcgttcataa atgggatcaa atgcagcatt ggttatgatg ctggatatac tctctgaacc
     9601 tttactatga gcagttacca gcgtggtttt gtaatcccct gacaatgttc tgcttaatcc
     9661 atatgctgct ccaaaagttc catccccttc aactgccgaa caatttttat gccgtacagc
     9721 acaggcatct cctaccgcca tacatgaaat ggttctgcca tcccaaaaat cgcttgaggt
     9781 tgttatcaca ttatcaatat catctattgt tattgatgca tctgctaatg ccttattgac
     9841 cgcttcccat gctaaatctg cataattaca ggctattttg tttttttcta ttgttgtcat
     9901 tcctatacca atgatagctg catccatact tttcctccta agtcagcggt ttgaaatgcg
     9961 aaatggcatt aattgttgca tttctatcat ctgcaaaaac agcctctact tccatcccta
    10021 tagtaatgtt atttacatcc acatcaccaa tataatgaag cattgcatta tccgcaccgt
    10081 ctatacaaat taaaccaaaa gcaaccggaa cttttttctg taaagctgcc agttgtcgta
    10141 aaactatagt atatgaaatc aattttcctt taggaccaac ctctttccat gcattggttt
    10201 cagtaaagca tgaagggcag gttttttttg gtggacaata aactttaccg cattgtgggc
    10261 attgtttccc caatattttt ttttcatccc ttaatgctat aaaatattta gatgcagttt
    10321 cacctgccca ccactgatag ggaacattga tctgactttt atataccgat ggttcaatac
    10381 catgaaatat atttcccata tttttatcct tttatcttcc tataatataa tgatgaggat
    10441 caacctcttc acgtaatatc cgcaattctt catccgctgg aggtttggtg tcatctatct
    10501 tctttgccca tttcaattca aaaccacaat tatcctgaac atcctgtcgg ctataacctg
    10561 gattaattga cagtaccatc atccactttg tttcatcatc aaatcccatt actgccatat
    10621 tggtaattat ccggtaaggt cctgtacctt cgggcaagcc ggcatcatat cgtgatctgc
    10681 caccttttaa atagcccggt gaagttataa aatcaagttt atcggtaaac ctgcgagcat
    10741 cctgcaccgt catcatcatc gtacgccagc acagtgacgc taaatcattt gcaccacctg
    10801 agccgggaaa gcgcacttta ggtttttgat agtcatcccc aatcattgtt gagtttatat
    10861 tcccatacat atctatctgt gcaccaccaa gaaatgtata atcaaccatt ccacgctgac
    10921 atgtctccat aacctctggc attgaacttg ccataattgc tttatgaaat gttcgtgaat
    10981 cccccactga tatgggcatt gtaggcaata tcggagcaac acctcctgct tcaaacaaaa
    11041 tacacaaatt tggtgaatat attttttggg aaagcattgc tgctgcgcag ggagcacctg
    11101 ttccaacaac aactgtcgcc ccatcttcta aatttcgtgc tgctacacat atcatgagtt
    11161 ccatagcatt atagtccata gctcacctcc ctatttatta ttgatcagat attctatggc
    11221 tctgagcttt ttcatttttt caataccacc attcaaattg aggtattcat aaaaatcatt
    11281 cactccataa atttgctcct gcaaaaatat ttctaattct tcaggatctc gttcaacttt
    11341 cagccatgtt ttgagatgct cttcatcgct aaaatattca tacggcatat taccggggta
    11401 actgccatac ggtacttcaa taattgcatc aacacaccag taaggaatga cagtcttttc
    11461 aggttctctt cgtatttcat catgtggtat aatccgttct gttgttatta ccactctttt
    11521 tgaagctttt gccagatcaa aatctgatac tgatgctcca tataccctgc aattcccata
    11581 gatatctgct tcatgaacat gaatagcggc aaaatctgga aacaaggctg gcatggctgc
    11641 atattttttt cccgtaaatg gacatgttat cgttttggca gcactgtgtt taaatgtgtc
    11701 tgttcccaat atattacggg taggtataaa agatataccc atagctgctg ctttgagtcg
    11761 ccatgaaagt gatgcatttg tccattcagt aagcttaatt gtgccgcttt caacagcacg
    11821 acgtgcattg ggcgatagtc ctcgagcctc caatcctatg atgtaagcaa catcacagcg
    11881 atcaatgcat ttacccgcta taaacacctg aaaatcatgt gttgacgtgt gaccggcaaa
    11941 tccgagatgt tttttatgct gacgcacaat ttcatgaaca attgctgttg gtatacggtt
    12001 tgcaccaaaa cctccaatgg caatataatc accatcttta atcatatcac gtacaacatc
    12061 tttaatctgt gccagcttcg gtgtcataga acgggattta ttcctgaaaa aagctctggc
    12121 tttatcggga tcaggatcgg taaacagttc atttttcccc gatagcatta caccttccgt
    12181 atcgttatcc cgagtaccta atgccatagt aacctcctgc ttatcattca aaataaagca
    12241 tacattcata aattgattga acatctaatc aataatatac gcttttaata aatctgaata
    12301 gtttttgcat cctcctcata taaggaatca atgattgact gatatttttg ctgaacagct
    12361 ttacgcttta ctttaagagt agcggtaagc tcacctgttt ctttggaaaa ttcattcttt
    12421 ataatgccca ctttttttac tgtttcatat cgagcaagct tttggttgca tttttctata
    12481 tgttcctcaa ctatcgtttg aaatcgtgga ttattgaaaa gatcatgaag gctattaaca
    12541 ttaataccct tttcttttgc aattcgctct gcctgttctt gacttatatt aaccagtgca
    12601 attaaatact ttcgcttatc accaataact atcgcctctg tgaatagagg atcggattta
    12661 ataagatttt caatcttttg tggtgcaaca ttcttgccgc ctgaagtaat aattaaatcc
    12721 ttcttcctgt ctgtaatcat taaaaaacca gcatcatcaa ataaacctat atcaccactc
    12781 atgaaatatc catcctgagt aaatgagtct tttgtctctt caggcatctt ccagtagcct
    12841 ttaaaaacat tatcaccttt aataagaatc tcaccatcat cagcaatttt gatatcaact
    12901 cccggcaatg gtctgcctac cgttccaatt ctataatcag caatattgct catggttgct
    12961 ggagcacagc attcggtcat tccataccct tcaataacca taattccggc tgaattaaaa
    13021 aataaaatta tttctcttgc tgttggagcg cctgaagctg tcatccagcg gactcttccc
    13081 cctaatgcat tttgcaactt cttaaaaatt attgcatatg ctattttata tttcaaggac
    13141 aaattaaatc ctggattttt tttctgctct ctcaattcac tgattttact gccaactttt
    13201 tgcccccaat aaaatacctt ttgcttaaaa ggtgattgct cctgtacctg tgatattatt
    13261 tgctgatata ccttttcaag aacacgggga actgccagca caactgttgg ccgtttttcc
    13321 tgaatatcct gtattaacgt atctaaactt tctgcatatg atgcagttat acctacatac
    13381 attccataaa agtgccctgc tactctttca aaaacgtggc taagcggtaa aaatggtaca
    13441 gtttgatcac tatcatatgc ataatgaggt tcaatggtat caagtgcctt tataactgcc
    13501 atgatatttt tatgggtaat catagctcct ttaggaacac cggtagttcc tgatgtatac
    13561 actatggttg caagatcatc ctgagcaata gtatcagcta atgatgcaaa tgcttcagga
    13621 tttttattat ataacacttt tcctttatca agaagctcat tataatccat aatcatggga
    13681 ttaacattaa cttcagttgt atctataaca ataatctttt ttaaaccagg ataacgcttt
    13741 gcagtataca aacccttttg taattgagcg ttattttcta ctattaaaaa ttttgaatca
    13801 gaatgggaaa ctatatactc aacttcatca tcaattaaag taggataaat agggactatg
    13861 caacctccag ctcccagaac gcccatatca gcataaagcc attccaaacg gttttctgaa
    13921 agcaatgata ccctatcacc tttttgcaat cccatgtcat ttaacgccaa tccaacatat
    13981 gcagcccttt tataataatc ttcccatgaa acactttccc atttgccatt taattttttt
    14041 tcaagagcta tgcgtgaacc atatttttgc actctattcc taaaaacctg attaatcgtt
    14101 ttttcctcca tgcgttgcct ccaatttctt aacaatgttt aaatattggt tttcttttat
    14161 ttaaaaatgc atcgatacct tcttttttat catgtgattc agctaacctc ccaaacaggg
    14221 tactttccaa tcgtaatcca tcttccagat tcattgagct acccttcatg actgcttcta
    14281 caatagatcg tacagcaatc tgtcctttct ttgcaattga agaagcaaac tttaaacttt
    14341 cttcaattaa ttcatcggga gcataaactc tattaaccaa tcctatttgt aatgcttcct
    14401 gcatattaat aaacattcct gttaacataa actccagtgc tttagccttg ccaagcagtc
    14461 gtggactacg ctgtgttcca ccaaatgccg gaataatacc cagcattatc tccggcaaac
    14521 ccatctgagc tgtattactt gcaatcctga tatggcagga aagagctatc tccatcccac
    14581 cacctaaaca caacccatta atcattgcaa taactggttt gggcgatagt tcacataatg
    14641 aaactattgc ctgtgctttt gttgtaattt ctaccccttt atcgaaggta ttaacttttg
    14701 cgacttcatt tacatcagca ccaactataa atgtagagcc tttccccgtg agcacgataa
    14761 catgcacttt atcatcggtt atcaatatcc tcataatttc ttcaaacgat tccattgttt
    14821 cagtatctaa agcattaatt ggtggattat taaaataaac tacagcaaca ttttcttttt
    14881 ctatctgata ataacattta tcattcataa t
//
LOCUS       DAOSOX010000040        14829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4066_length_14829_cov_9.012860, whole genome shotgun sequence.
ACCESSION   DAOSOX010000040 DAOSOX010000000
VERSION     DAOSOX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_14829_cov_9.012860"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLX22_04580"
     CDS             <1..512
                     /locus_tag="PLX22_04580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19204.1"
                     /translation="AKRGWKSSGIDIAPDCINYATTQLHVDAYCGDFLTFNFTSTYHL
                     ITLWATIEHLHYPDRFLEKIFSLLHDDGLLYISTCRSSIWSPRILFGKQWRYYNFPHH
                     LYFFSYATLAKLLQLKGFTITRFATYGSGIGKAGSTLRTVADGLAKKLYLGDMMLIAA
                     KKQKRTFNC"
     gene            525..1394
                     /gene="panC"
                     /locus_tag="PLX22_04585"
     CDS             525..1394
                     /gene="panC"
                     /locus_tag="PLX22_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOT19205.1"
                     /translation="MEIIHTIDALRDAIKKERTNNKRIGFVATMGYLHQGHLSLIHIC
                     KQHTDFQVMSIFVNKMQFNDPRDYDTYPRNYDRDFALAKEAGVDLIFLPDDSEMYQNR
                     LTYIDIEMMTSFLCGSARPGHFRGVLTVVAKLFNIVQPDVSVFGQKDIQQAVIIQKMV
                     KDLNFPLAIIVAPIIRESDGLAMSSRNVHLKGDDRTNATCMYQSLKKAEELIQHGIIQ
                     ANTIIPAMTDIIAAGKPKKIDYISIVDYETLQPVDSLKDKSVIAVAAFFGTTRLIDNM
                     IVEKIGDSFTCVY"
     gene            1382..1834
                     /locus_tag="PLX22_04590"
     CDS             1382..1834
                     /locus_tag="PLX22_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19206.1"
                     /translation="MRILINLLLIPFIVACGTSKPLTPQQALQQLSDSYIGGDMAKVA
                     QLLSTNSKKRIQYAIQLIGAMNQSQLSSFAKHYRISAASLKNLTIPQYLSLQRTIASI
                     EGDDVILQALQQPVVDITINNAQATVTVYNGMTLIFVKEGAYWYFEYE"
     gene            complement(1838..2599)
                     /locus_tag="PLX22_04595"
     CDS             complement(1838..2599)
                     /locus_tag="PLX22_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT19207.1"
                     /translation="MAQLIQSLERGLRILEILGKSDKPLSLGEITQFFDIDKSSIFRL
                     LSTLTQNNFVYQDAETKKYMLGFKIMELSGAFGEQTKIETFLRPVLKAVCDKTRQNTH
                     LAILHGQEVVFIAVEQPRNGISMHISVGTREPVHATALGKVLIAFAQKNEQELLLQGI
                     SFTHYTPHTIVDGNEYRNHLISIVQNRYAVDDEEYRLGVVCIAAPVFNHRGEVEYSIG
                     ISGPKDQISPHIADYITVVQDAGAECSRLLGMSSR"
     gene            3040..3447
                     /locus_tag="PLX22_04600"
     CDS             3040..3447
                     /locus_tag="PLX22_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT19208.1"
                     /translation="MFIDFSTINGTTVGRPFERELKVILSPDTNRSINDFTFILSTLA
                     PNGGCTDFHVHDTSGELMIFMSGSGKAWFEGMEYKIKPGTALYAPPGSEHKTMNTGNE
                     PLMIACIFIPAIASEYIVKSAEEANNAKEASNE"
     gene            3422..5563
                     /locus_tag="PLX22_04605"
     CDS             3422..5563
                     /locus_tag="PLX22_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT19209.1"
                     /translation="MQKRHPMNNAGFDFKKLHNELIASGNVILPEIESKHIVSRYAIP
                     TPKGKFVTTIAQLKEASTLLTFPVVCKVSGRLLHKTEIGGVITGIKSYDELKECFSML
                     DSSMKQKNIAGYNGILVEEQCHGHIEMIAGYTNDPVFGPTVMLGSGGIYTDLFKDVVF
                     HHAPVTEEDVMSMLSRLRCYPLLMGYRNIPTIDCKPLIQCFMNLNRLICDGARYIQSI
                     DFNPIIVDEKSCVVADASFTFYHAPIPDPFIVEKPAVQHLHTFFNPASVAVVGASTVV
                     NKIGNVIVDTLINGGFLGDIYPINPHQKEVLGLTAYHSINELAKSPDVVIIAVDLQMV
                     PEILDQMHAKGCHNAIIISGGGKELGGTREKLEKEIEAKAKCYDIRIIGPNCIGVFNS
                     LSRFDSFFYHRDRFTRPPQGTISFITQSGTWGVTFMELAQTTGLHIMVSYGNRVDVDE
                     GDLISYLMDDAGTGVIGSYIEGLQKGKKYAEAIAHAIKKHKPVVVYKTGRSDISSQAA
                     ISHTGAYGGSYTLYHDMLQDAGAILTDSLHECFAACMALSMQPPAKGNRVALVSNGAG
                     PMVNALDLFPQKNLVLAPLSDTTKKTMREHFSFFYIVHNPVDVTGSATSDDYDFVLQQ
                     LMNDDGVDIIMPFFVFQNTPLDEAIIEKMKRHSDLQKKPIVCSCTEGTYSQRMNNRLI
                     HAGIPVFHEISHWVVAVYALMQWGKIIQHEV"
     gene            5565..6401
                     /locus_tag="PLX22_04610"
     CDS             5565..6401
                     /locus_tag="PLX22_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006091262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT19210.1"
                     /translation="MQKLIITAALTGGIHGKEANPALPEQPDEIIQAAYDCYNAGAAI
                     VHIHARDRDGNQTASKDIFATINNGIKSKCPVIIQNTTGGPGLPVEERIASLDAHPDS
                     ASLNCGSVVFFYQGKQLPFHNYRHEIEAFAQAMLQRTIKPELEVYNPSMFLEVENLIA
                     KNLLALPYYINLVMGVNGMGGYPGTPENFLTMLRHLPDNSICNVSGIGKYQLTMNTMA
                     ILTGCNVRTGLEDNVFYHKGKLATSNAQLVERVVRLAKELGRDIATPDEARTLLRIGG
                     AS"
     gene            6398..7024
                     /locus_tag="PLX22_04615"
     CDS             6398..7024
                     /locus_tag="PLX22_04615"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOT19211.1"
                     /translation="MKIAVINETSAADKNADIMQALQNRGHEIINAGMTISGQSPEIT
                     YIHTGLMAALLLNAKRVDLVVGGCGTGQGFLNAVMQYPAVMCGHIVTPLDAWLFAQIN
                     GGNCISLMLNQGYGWASEQNLTFIFDALFSVQWGNGYPAHRKESQQSSRQLLSAISNL
                     THASMAHIMQALPDEVVKPALEYPGFIDSMDVPSLDDELKHAIVARIS"
     gene            7190..9055
                     /locus_tag="PLX22_04620"
     CDS             7190..9055
                     /locus_tag="PLX22_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT19212.1"
                     /translation="MNTGIQQRAAAIARHSSLHKAVLQGTIAQYQDITVSEALVLGLL
                     QQGVTRYFGIFGHGSTAIAEVLRIYEYHGLVHTYNLRHETAASHAATMLNMLTGEVAA
                     VVTSIGPGALHAFAGSLASASNGAGVYHIYGDETTHDEGFNMQQIPKDEQALFLKLCS
                     VMGNAYALYEPWSVISALKRGAVCTKGLPFAQPFFLLAPMNVQPAVLTHFNLLQLPHA
                     HRHVPVVCYDDAVFEKATALAKNAKRVTIKIGNGARGCGSYIETLANLLDAAIVSGPS
                     SAGIVPYTNPRYMGVGGSKGSLSGNFAMEADCIIVIGARGVCQWDCSGTAWKNARSIV
                     NFNVNPFHAAHYNRCVSVVGDAQGNLQRWIEHLRANGFSMGDSHSEWFLALQEKKAQW
                     QALKQQRFDCPVLFDEMWKRDVLTQPAAIKTACDFADEHNYIKVFDAGDVQANGFQII
                     EDKHEKQTITETGASYMGFASSSVLVSGIVDKKMMAFCGDGSFIMNPQILIDGVEHGA
                     NATIIIFDNRRMAAITGLQYAQYGSEYKTSDSVAVDYVAMAHSVHGVQGIYGGYTPSE
                     LMQALQKADQYRGLSLIHVPVYYGQNELGGMGVFGSWNVGSWCSQVQEEYHRQGW"
     gene            complement(9153..10604)
                     /locus_tag="PLX22_04625"
     CDS             complement(9153..10604)
                     /locus_tag="PLX22_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT19213.1"
                     /translation="MKQYRWFRDNRCRVCGQCLSFCPVVSLPAGAARRDRIIAREGSI
                     NESLSLSYCTTCNACNVICPTQSHPYELVLERYDEAQRNKGLPGIARLVFPNEYLNIW
                     AITGALLTRKEKHFLKQWENNLHEKHSTIMLTGFYTNLVPFLLDHVMFQNSTIPIAGS
                     EGLWGCGGDAHKLGLVDITEKVALLLKNKLHSMKVKKVICFMEAEAAMLQEILPQYYG
                     IDYNITIIPIEEWLLQQIKQGDITISAPLAIRIAVHDNCMSRYLNGKPQKTMREIVKR
                     CGGLSVEMEHAKEKSLCCGWAATIPSLYRNASLFGIALYLLYSLDKRLCEGVDAKADV
                     MVTGCPACYIFLSVAQYVTRRKIKIMHLTQVVSLAAGQPHNAMMDRRVRDILAGIIFF
                     LLRWISSRSVREHFHPASPQTDDVQTIPVLNKSDAKTLQRIRSILDSPLLTNRITQWL
                     IAMMVRLAVRLYGLCLAFKRKQFIRKQCSKVSG"
     gene            complement(10607..>11809)
                     /locus_tag="PLX22_04630"
     CDS             complement(10607..>11809)
                     /locus_tag="PLX22_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT19214.1"
                     /translation="SFTSPFDISRCKECGECLHQCPVLQMKPDEAISERKKLNRGRVS
                     AKIMKRCTTCFACNLVCPQGCNPAQQIIDLWHNAYRSKGLPLRARYFDPNHSTNFRTY
                     VVDRLPYDERALIEKWKDTSECAEILYPGCNIITAPYLTQTSLLEGLEIRGSLDVCCG
                     ETYYRMGLIDEMYKVAERLERYFKKLGVRKMIIPCTAGRNMFTNVLPSSGVKFDFEVV
                     HILPWLWERMEKGEIVIKEKLNMTVTVQESCYGKIFGNDYMDIPRKILNSIGVQVIEE
                     KLIRSHALCCGIAGGFSVKSGYHPVDIAHETFRSLGLAKQTGADAIAVYCAGCLQMLT
                     VGQILYPFNRMPVYHILELLQMAIGEKPARLNKNRAQTLFAGVLKHQVPLLFSLNRHR
                     VDVKKLDF"
     assembly_gap    11810..11909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12139..13986)
                     /locus_tag="PLX22_04635"
     CDS             complement(12139..13986)
                     /locus_tag="PLX22_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT19215.1"
                     /translation="MNLKKLIRNYLSFVNFSTMIQLTMHWLWFRRKRVFFIFEGREYT
                     YNEVYEQSKRYATFFLSMRKKLIESGRLHDNQRLSFGVYMDNNPELIFASFGAGLSNS
                     ILFAINTGFRGETLAKVIEQANISFLIINESTVNEVEPIINSGTVIHADDVYFAGNNN
                     GYTAQGFHSLEEAVDHAQHDTIPKNYRTKIDNFSPVIVIYTSGTSGLPKGVPCTHIKL
                     VGAGFIVQFAVHLNKNDRGYISMPLFHSNAWYIGILPQLIVGGSFVLKRRFSASAFEE
                     DMLRYGVTFLNYVGQPLHYIIEALERKYGSGEAVEAALAHHPRNKFRKAYGNGATVTD
                     RIKLKRYLGMEHIYEIYGSTEAVITTANLPGDPIDSVGRVDSSIKILNEEGKECEPGI
                     VDENGALINYDKAVGEICRKVGDSNLRFEGYFGNEKATSQKFRDGWYHSGDLGHIRII
                     NGRRYLFFNGRTDDWIRKDGENFSAENVLDFAHRIPCVDIAIAYGVPSEVSDEKVMIA
                     IQLKDGASFNPDEVYRWFMQQQKEGGMDPKWMPDYIRIIDKFSVTNTQKIMVRPFKKE
                     NFNLDSNPTMEIYFRQRGDNTYRLLTPDEFVKIKEAFKANGREAVLSQN"
     gene            complement(14051..14191)
                     /locus_tag="PLX22_04640"
     CDS             complement(14051..14191)
                     /locus_tag="PLX22_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOT19216.1"
                     /translation="MAVISVRFNTKEEKIVKILKKHYNCDASTLIKKSLWELHEDIKI
                     KR"
     gene            complement(14182..14316)
                     /locus_tag="PLX22_04645"
     CDS             complement(14182..14316)
                     /locus_tag="PLX22_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19217.1"
                     /translation="MKGSFKRNYYRLRKEKFRAMFFIENDDFYIVYIGKREEVYDQWQ
                     "
     gene            complement(14313..14456)
                     /locus_tag="PLX22_04650"
     CDS             complement(14313..14456)
                     /locus_tag="PLX22_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19218.1"
                     /translation="MRRKFKKSLKKGDIPAEIMKRFHNAFLSLDLTKDLNLFDVNYLM
                     LKR"
     gene            complement(14539..>14829)
                     /locus_tag="PLX22_04655"
     CDS             complement(14539..>14829)
                     /locus_tag="PLX22_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19219.1"
                     /translation="EGIFYGFFYFSHKLAAACAGFITGIGLTLCGYLQPLLVGNTLTS
                     QMQAEGALTGIRLLSTIIPMLLIILGIVFIWLFPIDEKMHRKIVEEIGSGVM"
BASE COUNT         4188 a         3249 c         3114 g         4178 t
ORIGIN      
        1 tggcaaaacg cggctggaaa tccagtggca ttgatattgc acctgactgc atcaattacg
       61 caaccacaca gctccatgtt gatgcctatt gtggtgactt tttaacattc aattttacca
      121 gcacctacca cctgattacc ttatgggcaa ccattgagca cctccattac cccgataggt
      181 tccttgaaaa aattttttca ttgctgcatg atgatggctt gttgtatatc tcaacctgcc
      241 gcagcagtat atggtcgccc cgcattttgt ttggtaagca gtggcgatac tataatttcc
      301 ctcatcacct ttactttttt tcgtatgcaa cgcttgcaaa attgttgcaa ttaaagggct
      361 ttaccattac acggtttgct acctatggta gcggtattgg taaagcaggc agcacgctta
      421 gaaccgttgc cgatggattg gcaaaaaaat tgtatctggg tgacatgatg ctcattgctg
      481 caaaaaagca aaagcgtaca tttaattgtt gaggtaaaac aaccatggaa attatccata
      541 ccattgatgc attacgcgat gcaataaaaa aggagcgcac caacaataaa aggattgggt
      601 ttgttgcaac catgggctat ttacaccagg ggcatctgag ccttatacac atttgcaaac
      661 agcacacgga ttttcaggtt atgagtatct ttgttaacaa aatgcaattc aacgacccca
      721 gggactatga tacctatccg cgtaactatg accgggactt tgcactggca aaagaggctg
      781 gtgttgattt gatatttctt cctgatgata gcgagatgta tcaaaatagg cttacctata
      841 tagatattga aatgatgacc agctttttgt gtgggtcggc ccgccccggc catttcagag
      901 gtgttttaac cgttgttgca aaacttttca acatagtaca accggatgtt tcggtgtttg
      961 gtcaaaagga cattcagcaa gccgtcatta ttcagaaaat ggtaaaggac ttaaattttc
     1021 cacttgccat tattgttgca cccattatcc gcgaaagcga tgggcttgca atgagctcgc
     1081 ggaatgttca tttaaaaggg gatgacagaa ccaatgcaac ctgtatgtac caatcattga
     1141 aaaaagctga agagctcatt cagcacggta ttatacaggc taacacaatc ataccagcta
     1201 tgaccgacat tattgcagca ggtaaaccaa aaaagattga ttatatttcg atagttgatt
     1261 atgaaacatt gcaacctgtt gactctttaa aagataaatc ggtgattgct gtagcagcat
     1321 tctttggaac aacacgtctt attgataata tgatagtaga aaagatagga gattcattta
     1381 catgcgtata ttgataaatt tactgctcat tcctttcatt gttgcctgcg gtacatcaaa
     1441 accacttaca cctcagcagg cattgcaaca attatcagat tcatatattg gtggcgacat
     1501 ggcaaaggtt gcgcaactgc tttctacaaa tagtaaaaaa cgaatacaat acgcaataca
     1561 gctcataggt gcaatgaatc aatcgcaact gtcttccttt gcaaaacact accggataag
     1621 tgccgccagt ttaaaaaatc taaccatacc gcaatatctg agcctgcaaa gaacaattgc
     1681 atcaatagaa ggtgacgatg tcattcttca ggcattgcag caacctgttg ttgatataac
     1741 aataaacaat gcacaggcaa cggttaccgt ttacaatggt atgactttaa tctttgtaaa
     1801 agaaggtgca tactggtatt ttgaatatga gtgatagtta ccgggaagac atacccagta
     1861 atcgtgagca ttccgctccg gcatcctgaa caacagtgat gtaatcagca atatgcgggc
     1921 ttatctgatc ttttggtccg gatatcccta ttgagtattc aacctcgcca cggtggttga
     1981 ataccggtgc tgcaatacac acaacaccca atcggtattc ttcatcatca accgcatacc
     2041 tgttttgaac aattgatatt agatggttac ggtattcatt gccatcaact attgtatggg
     2101 gagtatagtg tgtaaacgat ataccctgta atagtaattc ctgctcattt ttttgtgcaa
     2161 aagcaatcag cacctttcct aaagcagtgg catgcacagg ttcgcgggtg ccaacggata
     2221 tgtgcatgct tatcccattg cgcggctgtt caacagcaat gaataccacc tcctgtccat
     2281 gtaatattgc aaggtgagta ttctgtctgg ttttgtcgca tacagctttt aaaaccggcc
     2341 gtaaaaacgt ttcaattttc gtttgctcgc caaacgcacc tgaaagctcc ataatcttga
     2401 aaccaagcat atattttttt gtttcagcat cctgatatac aaaattattt tgtgtcagcg
     2461 tgctcaataa tctaaaaatt gatgatttgt cgatatcaaa aaattgcgtt atttcgccca
     2521 gtgaaagcgg tttatcggat ttgccaagta tctctaaaat acgcaagccc cgttctaatg
     2581 attgaattaa ctgcgccaca ttccatctcc ttatgtttca tactatgcaa tgacaatgca
     2641 cgctatagtg tcaagtacta atttttttat gataaatgag caatttttat gctttctcca
     2701 aaaaagttgt tgacatttat gcaataatat tgcataatac acaatatcag caaggcatat
     2761 atgctatcgt tgaaattaaa ggaaacggtg ctgaaggaaa cctgtcatgg cgacagctca
     2821 taatgcagca tcacatatat gcatgtttcg cgcttcagaa gctatggtaa tggcaccatg
     2881 atatatccgg tcaggtctct ggatttacca taattgaaat tctgagatag tcattgtggc
     2941 aggtaaatgc ttatgatgtc gatgagtcga tgaagagtaa agccccctat agcagggagg
     3001 ttcatcatag cacattgtaa tgatggaggt acatggatta tgttcataga tttttcaaca
     3061 atcaacggta ccaccgttgg caggccattt gaacgggaat taaaagtgat actatcaccc
     3121 gatacaaacc gctcaattaa cgattttacc ttcatcctgt caacgcttgc tccaaacggc
     3181 ggctgtactg attttcatgt acatgataca tcgggcgagc ttatgatctt catgtccggt
     3241 agtggcaaag catggtttga aggcatggaa tacaaaatta aaccaggcac cgcactgtat
     3301 gctccgccgg ggtcagagca taaaacaatg aacacaggaa acgaaccgct tatgattgcc
     3361 tgcatcttta tccccgccat cgcatcagag tatattgtca aaagtgcgga ggaagctaac
     3421 aatgcaaaag aggcatccaa tgaataacgc cggttttgac tttaaaaagc tgcataatga
     3481 gcttatagca tccggcaacg taatcctgcc tgaaatagaa tcaaagcaca tagtatcacg
     3541 ctatgccatt cccacaccaa aaggaaaatt tgtaacaact atcgcacagc taaaggaagc
     3601 ttcaaccttg cttacattcc ccgttgtgtg caaggtaagc ggacgattgc tgcacaaaac
     3661 cgagattggt ggtgtcataa caggtattaa aagttatgac gaattaaaag aatgtttttc
     3721 catgctcgac agctccatga aacaaaaaaa catagcaggc tataatggga tacttgttga
     3781 agaacaatgt catggccata tagaaatgat tgccggctat accaatgacc ctgtgtttgg
     3841 acctaccgtc atgctgggtt ctggcggcat ttatactgat ctgtttaagg atgtggtgtt
     3901 tcaccatgcg ccggttactg aagaggatgt gatgtccatg ctttccagat tgcgatgcta
     3961 tcctcttctg atggggtata gaaatattcc aacaatagat tgcaaaccat tgatacaatg
     4021 cttcatgaat ttaaacaggt tgatttgtga tggcgcccgg tatatacaat ccatagattt
     4081 taatccaatt attgttgatg aaaaaagctg cgttgttgcc gatgcgtcat ttacctttta
     4141 ccatgcaccc atccccgatc ctttcattgt tgaaaaacct gctgtgcagc atctgcatac
     4201 tttttttaat ccagcaagtg ttgccgtggt aggcgcttca acggttgtca acaaaatagg
     4261 caacgttatt gttgacaccc ttatcaatgg gggctttcta ggagatatct atcccatcaa
     4321 tccccatcaa aaagaggtgc ttgggcttac cgcatatcat tcaatcaatg agcttgcaaa
     4381 atcacctgac gtcgttatca tcgcggttga tttacagatg gtacctgaaa ttcttgacca
     4441 gatgcatgca aaaggctgcc ataatgcaat cattatctcc ggcggcggca aagagctggg
     4501 gggcacgcgt gaaaagctgg aaaaagaaat tgaagcaaaa gcaaaatgct atgatatacg
     4561 cattattggg ccaaattgca ttggcgtgtt taactcactc tcacggtttg attcattctt
     4621 ttatcataga gacaggttta cccgcccgcc gcagggaacc atcagcttca taactcaaag
     4681 cggcacatgg ggagtcacat ttatggaact ggcacaaact acaggtttac atataatggt
     4741 aagttacggc aaccgtgttg atgttgatga gggtgatctt atttcgtatt tgatggatga
     4801 tgccggcacc ggtgttattg gcagctatat tgaaggtctg caaaagggaa aaaaatatgc
     4861 tgaagcgata gctcatgcaa taaaaaaaca caaaccggtg gtagtgtata aaacaggcag
     4921 gagcgacata tcgtcacagg ccgcgatatc gcacaccggg gcgtatggcg gaagttatac
     4981 gctgtaccat gacatgttgc aggatgccgg tgcgatactt accgatagcc tgcatgaatg
     5041 ctttgcagca tgtatggcgt tatccatgca gccgcctgca aaaggcaacc gtgttgcact
     5101 ggtttcaaac ggtgcgggac caatggtcaa tgcacttgac ctcttcccgc agaaaaacct
     5161 tgtgcttgcg ccgctgtcag atacaacaaa aaaaaccatg cgggagcatt tcagcttttt
     5221 ttacattgtg cataatcctg ttgatgtgac tggttcagcc acctccgatg attatgactt
     5281 tgtattgcag cagcttatga atgacgacgg cgtggatatc attatgccgt tttttgtttt
     5341 ccagaataca ccgcttgatg aggcaatcat tgaaaagatg aaaaggcatt ctgatttaca
     5401 aaaaaaaccc attgtttgca gttgcactga aggcacgtat tcacaacgca tgaacaatag
     5461 attaatacat gcaggcatac cggtgtttca tgaaatcagt cactgggttg ttgcagtgta
     5521 tgctttaatg caatggggaa aaattattca gcacgaggtg taatatgcaa aagcttatca
     5581 taaccgcagc cctgaccggc gggattcatg gcaaggaagc aaatccggca ttacctgaac
     5641 agcccgacga gattatacag gctgcgtacg attgctacaa cgcaggagcg gcaattgtcc
     5701 acatccatgc gcgtgacagg gatgggaacc aaactgcatc gaaagatatt tttgctacaa
     5761 tcaacaacgg tattaaaagc aaatgtcctg ttatcattca aaatacaacc ggcggtccgg
     5821 ggcttcctgt agaggagcgc attgcatcgc ttgatgcaca tcccgactcg gcttcgctca
     5881 attgcggctc ggtggtgttt ttttatcagg gcaaacagct ccctttccat aactatcgcc
     5941 atgaaattga agcctttgcg caggcaatgt tacagcggac cataaagcct gaactggagg
     6001 tttacaaccc ttccatgttc cttgaggttg aaaacctcat tgcaaagaat ctacttgctt
     6061 taccatatta tataaattta gtcatgggcg tcaatggcat gggaggatat cccggtacgc
     6121 ctgaaaattt tttaacgatg ctgcgtcatc taccagacaa cagtatttgc aatgtatccg
     6181 gtattggcaa atatcagctt accatgaaca ccatggcaat acttacaggt tgtaatgtta
     6241 gaacagggct tgaagacaat gtgttttatc ataaggggaa gcttgcaaca tcaaatgcac
     6301 agttagtgga aagggttgtg cggcttgcaa aagaacttgg cagagacatt gctacccctg
     6361 atgaggcacg tacactcctg aggataggag gagcatcatg aagattgcag ttataaacga
     6421 aacaagtgcc gcggataaaa atgctgatat catgcaggcg ttacaaaaca gaggacatga
     6481 aattatcaat gcaggcatga ccataagcgg acagtcgccg gaaattacct acattcacac
     6541 gggattgatg gccgctttgc tgttaaacgc aaaacgggtt gaccttgttg ttggagggtg
     6601 tggtaccgga cagggatttt taaacgctgt aatgcaatat cccgctgtaa tgtgcggaca
     6661 tattgtaaca ccgcttgatg catggctttt tgcgcagatt aatggcggca actgtatctc
     6721 gcttatgctt aatcagggat acgggtgggc atcagagcaa aaccttactt ttatatttga
     6781 cgcattgttt tcggttcaat ggggaaacgg ctatccagcg caccgtaaag aatcacagca
     6841 aagctcccgc cagcttttat ctgccatttc aaatctgacg catgcttcca tggcacacat
     6901 tatgcaggca ttgcccgatg aggtggtaaa gcctgcttta gaatatcctg gctttattga
     6961 tagtatggat gtaccatcgc ttgacgatga acttaaacat gccatcgtag caaggatatc
     7021 gtagttcacg atggctggat tatggacaat catacgggat aaaaatattt ggtgacagcc
     7081 atcctgaacc atattcctgg tttcaggcgc tctgttacct atctggattc cgggtcaagc
     7141 ccggaatgac attgatagaa taatgctatt tttcaaagga gctaccatca tgaacacagg
     7201 catacagcaa agagcggctg ccattgcacg tcacagctca ttgcacaaag ctgttttaca
     7261 gggaactatc gcacagtacc aggacatcac tgtttcagaa gctcttgtac ttggattgtt
     7321 acaacagggt gtgaccaggt attttggcat ctttggacat ggttcaaccg ccattgcgga
     7381 ggtcctccgt atttacgaat atcacggact ggtgcacacc tataacctca gacacgagac
     7441 ggcagcttcg catgccgcta ccatgctcaa tatgctcacc ggcgaagtgg cggcggttgt
     7501 tacgtcaata gggcccggtg cattacatgc atttgcaggt tcgctggcat cagcatcaaa
     7561 cggtgccggc gtttaccaca tatatggcga tgaaacaacc catgatgaag gcttcaacat
     7621 gcagcaaata ccaaaggatg agcaggcgct gttccttaag ctgtgcagtg tcatggggaa
     7681 tgcctatgca ctgtacgagc catggagcgt tatctctgcg cttaaacgcg gagcggtgtg
     7741 cacaaaaggg ctgccgtttg ctcaaccgtt ttttttgctt gcgcccatga atgtacagcc
     7801 ggcagtgctt acacacttta atcttttgca gcttccccat gcacatcgcc atgttcctgt
     7861 tgtgtgctac gatgatgctg tttttgaaaa ggcaaccgcg ctggcaaaaa atgcaaagcg
     7921 ggtaaccatt aagattggta acggcgcacg gggttgcggc agttatattg aaacgcttgc
     7981 caacctgctt gatgccgcta ttgtcagcgg tccctcaagc gccggcatcg ttccctacac
     8041 caatcccagg tacatgggcg tgggcggttc caagggttca ctgtccggca actttgcaat
     8101 ggaagctgac tgcatcattg ttataggtgc acgcggcgtg tgtcagtggg attgctcggg
     8161 cactgcatgg aagaatgcgc gttccatcgt caactttaat gtcaatccat ttcacgcagc
     8221 tcactataac cgctgtgttt cagttgttgg cgacgcacag ggtaacctgc aacggtggat
     8281 tgagcacctg cgggctaacg gcttttctat gggcgatagt cactctgaat ggtttttagc
     8341 attacaggaa aagaaagcac aatggcaggc attgaagcag cagcgctttg attgcccggt
     8401 actatttgat gagatgtgga agcgggatgt gctcacacag cctgcagcaa taaagacagc
     8461 atgcgatttt gcggatgaac acaattatat aaaagttttt gacgccggcg atgtgcaggc
     8521 taatggcttc cagattattg aagataagca cgagaagcaa accataaccg agactggagc
     8581 atcgtatatg ggctttgcat catcatcggt gcttgtttcc ggcattgttg acaaaaagat
     8641 gatggctttc tgcggtgatg gcagttttat aatgaatcca cagattttaa ttgatggtgt
     8701 tgagcacggt gccaacgcta ccatcattat ttttgacaac agaaggatgg cagccatcac
     8761 cggcttgcag tatgctcagt atggcagcga atacaaaaca tcggatagtg ttgctgtaga
     8821 ttatgttgcg atggctcatt cggttcatgg tgtgcagggt atctatggcg gttacacccc
     8881 ttctgaattg atgcaggcat tacagaaagc agatcaatac aggggactat cgctcataca
     8941 tgttccggta tattatggtc aaaacgagct tggcggcatg ggtgtatttg gcagctggaa
     9001 tgttggcagc tggtgttcac aggttcagga ggaatatcac cgacagggct ggtgaacagg
     9061 tggggacaac tcctgtccgg ttactccttg cagtaactgc tatttacgca atgcatccct
     9121 gtcactgccg ccatgagctt ttttaccggt acctatccag acacctttga gcactgtttg
     9181 cgaatgaatt gttttctttt gaatgcaagg catagcccat acaaacggac agccagccgt
     9241 accatcatcg caatcagcca ttgcgtgata cgatttgtta acagagggct atccagaatg
     9301 ctgcgtatcc tttgcagagt ttttgcatcg gatttgttaa gtacagggat tgtctggaca
     9361 tcatcagttt gaggcgatgc aggatggaaa tgctcacgga cagaacggga agaaatccat
     9421 cgcagcaaaa aaaatataat ccctgcaaga atatcccgga cacgcctatc catcatagca
     9481 ttatgcggct gccctgctgc taacgaaaca acctgagtaa gatgcattat ttttattttt
     9541 cgtctggtta catactgcgc aactgaaagg aagatgtagc aagcagggca gcctgtcacc
     9601 attacatcag cctttgcatc cacaccctcg cagagccttt tgtcaaggct atacagcaga
     9661 tacagggcaa ttccaaaaag tgaggcattg cggtaaagcg atggtattgt ggctgcccat
     9721 ccgcagcaca gcgatttttc ttttgcatgc tccatctcaa cagataagcc gccgcaccgt
     9781 tttacaatct cgcgcattgt tttttgcggt ttgccgttga ggtatcgtga catgcaatta
     9841 tcatgcacgg caatccgtat ggcaagggga gcacttattg taatatcgcc ctgttttatt
     9901 tgctgcaaaa gccattcttc tatcgggatt atagtgatat tataatcaat gccataatac
     9961 tggggaagta tctcctgaag catggctgcc tcggcttcca taaagcagat aaccttttta
    10021 accttcatgg aatggagctt gtttttcaac agcaaagcca ccttttcagt gatgtcaaca
    10081 agtcccagct tatgagcatc accaccacat ccccataacc cttcactccc cgcaatggga
    10141 atggtggaat tttgaaacat tacatggtct aaaagaaaag gaacaaggtt tgtataaaat
    10201 ccggttagca taatggtgct atgcttttca tggagattgt tttcccactg ttttaaaaag
    10261 tgtttttctt tccgagtcaa aagagcccct gttattgccc atatattcag atactcattg
    10321 gggaaaacca atcgtgcaat ccccggcagg cctttattgc gctgggcttc atcataacgt
    10381 tccagaacaa gctcgtatgg atgtgattgt gttgggcata taacgttgca agcgttgcag
    10441 gttgtacaat atgatagcga tagtgactca ttaattgagc cttcccgtgc aatgattcta
    10501 tccctgcgtg ctgcaccggc tgggagcgaa actactggac aaaaagaaag gcattgacca
    10561 catacacggc agcgattgtc acgaaaccag cggtattgtt tcatgcttaa aaatccagct
    10621 tttttacatc aaccctgtga cggtttaacg agaacagcag tggtacctga tgttttaaaa
    10681 cgccagcaaa caatgtttgt gctctgtttt tgttaagccg tgcaggtttt tcgccaattg
    10741 ccatctgcaa aagttccaga atgtgataca ccggcattct attgaatgga tataaaattt
    10801 gccccaccgt aagcatctgg aggcaacctg cacagtaaac tgcaatggca tcagcacccg
    10861 tttgtttagc taacccaaga gagcgaaacg tttcatgagc aatatcaaca ggatgatatc
    10921 ccgatttaac actgaagcct ccggcaatgc cacaacagag agcatggctt ctgatcaatt
    10981 tttcttcaat aacctgtaca cctatgctgt tgagaatctt tcgcggaata tccatataat
    11041 cattgccgaa aatcttgccg tagcatgatt cctgtactgt cacagtcata tttaattttt
    11101 ctttgattac tatctcacct ttttccatcc gttcccatag ccagggaagt atatgcacaa
    11161 cctcaaaatc aaatttaacg ccagatgatg gaaggacatt ggtaaacatg ttgcgccctg
    11221 ctgtacaggg gatgatcatc ttccgcacgc ctaacttttt aaaatatcgc tccagacgtt
    11281 ccgccacttt gtacatctca tcgataagcc ccatgcggta ataggtttcg ccgcagcaaa
    11341 catcaaggct tcctctaatt tccagccctt ccagaagcga tgtttgtgtt aaataagggg
    11401 cggtaatgat attgcagccg ggatacaata tttccgcaca ttcagaggta tccttccatt
    11461 tttcaatcag tgctcgctcg tcatacggca gcctgtcaac gacataggtg cggaagtttg
    11521 tactatgatt ggggtcaaaa taccttgctc tcagcggcag acctttactg cggtatgcat
    11581 tatgccatag gtcaattatc tgctgagcag gattacatcc ctgcggacac acaaggttac
    11641 aggcaaaaca ggtggtacac cgcttcatta tttttgcaga aaccctgcct ctgtttaatt
    11701 ttttcctttc gctgatagct tcatcaggtt tcatctgtaa aaccggacat tggtgcaagc
    11761 attccccgca ttctttgcat cgtgatatat caaatgggct tgtaaagctn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng caagcattcc ccgcattctt tgcatcgtga
    11941 tatatcaaat gggcttgtaa agcttcgcaa ttgttccata tccaattcct ttaccaatca
    12001 aggatgatga aactacacca tcatcgtaac attgtcataa acaatatgca acaaataaaa
    12061 aacgttactc cttcaccagt tccagaataa ggttcattaa aaaactacac aatcatttat
    12121 ccataatcac agattttttt agttttgtga taaaacagcc tccctgccat ttgccttaaa
    12181 tgcttctttg attttaacaa actcatcagg tgttaaaagc ctataggtat tgtcgcctcg
    12241 ctggcggaaa taaatttcca tggtagggtt gctatcaaga ttgaagtttt cttttttaaa
    12301 gggcctcacc ataatttttt gtgtattggt cacagaaaat ttatcaatta ttctaatgta
    12361 atccggcatc cactttgggt ccatcccgcc ttctttctgc tgctgcataa accaccggta
    12421 cacttcatca ggattaaaag atgcgccatc tttaagctgt atggcaatca taaccttttc
    12481 atcggaaacc tcgctgggaa cgccataagc aattgcaatg tcaacgcatg gaattctgtg
    12541 tgcaaaatca agaacgttct cagcagaaaa attttcgcca tccttgcgaa tccagtcatc
    12601 tgttcggcca ttgaaaaaca aatatcgcct gccattgatg attcgtatgt gccccagatc
    12661 tcccgaatgg taccacccat cacgaaattt ttgactggtt gccttttcat tcccaaaata
    12721 tccttcaaat ctaaggttac tatcgccaac cttacggcat atctcgccca cggctttatc
    12781 ataattaatc agagctccat tttcatctac tatgcccggc tcgcactcct tcccttcttc
    12841 attcaatatt ttgatagatg aatcaaccct gccaacagaa tcgattgggt cccccggaag
    12901 gttggcagtg gtaataaccg cttcggttga accataaatc tcgtaaatgt gctccatgcc
    12961 caggtagcgc tttaatttta tacgatcggt caccgttgca ccgtttccat acgctttgcg
    13021 aaatttattg cgcggatgat gtgccagcgc tgcttccacc gcttcaccgc tgccatactt
    13081 gcgctccagt gcttcaataa tgtaatgcaa tggctgccca acatagttaa ggaaggttac
    13141 tccatagcgc agcatatcct cctcaaaggc gctggcggaa aaacgccttt tcaaaacaaa
    13201 gcttccgcca acaatcaatt gcggcaaaat tccaatatac catgcattgg aatgaaacaa
    13261 cggcatggaa atataaccgc gatcattttt attgagatgg acggcaaatt gcacaataaa
    13321 tcctgctccc accagtttaa tatgggtaca gggaacgcct ttgggcagcc ctgatgtgcc
    13381 ggaggtatag atgacaatga cggggctaaa attatcaatt tttgttcgat agtttttggg
    13441 gatagtgtca tgctgagcat ggtcaacagc ttcctcaagg gagtggaacc cttgagcagt
    13501 gtaaccatta ttgttgcctg caaaatagac atcatctgca tgaatcaccg tgccactgtt
    13561 gatgattggt tcaacctcat ttaccgtact ttcattaata atgagaaaag agatgtttgc
    13621 ttgctctata acctttgcta atgtttcccc tctgaatccg gtattgatag caaaaaggat
    13681 ggagttgctc aaacctgcac caaatgaagc aaaaattaat tcgggattgt tatccatgta
    13741 aacgccaaac gaaagccttt gattgtcatg cagccttcca ctttcaataa gctttttgcg
    13801 catgcttaaa aaaaatgttg catagcgctt tgactgctca tacacctcgt tatacgtata
    13861 ttcacgacct tcaaagataa aaaagacccg ttttcgcctg aaccagagcc agtgcattgt
    13921 taattgaatc attgtgctga aattgacaaa gctcaaataa ttgcgtatca gttttttaag
    13981 attcatgtat aatgccctcc tgttataaac tttgtctttc cacccttttc acgtttttca
    14041 aaggcctcta ttatctcttt atttttatat cctcatgtaa ttcccacagg gattttttta
    14101 tgagcgtgga agcgtcacag ttataatgtt ttttaagaat ctttactatc ttttcttctt
    14161 ttgtattaaa tcgtacactt attactgcca ttgatcatat acctcctctc gctttcctat
    14221 ataaacaata taaaaatcat cattttctat aaagaacatt gccctgaatt tttctttccg
    14281 taatctataa taatttcttt taaaacttcc tttcatcttt ttaacatcaa ataattaaca
    14341 tcaaataaat tcaaatcttt tgtcaaatca agcgataaaa aggcattatg aaatcgtttc
    14401 attatctctg ccgggatatc acccttttta agtgattttt tgaacttcct tctcatacta
    14461 tatcgcatac tagagcatgt ttacgaggta tacattaaag acaatttgtc aattgttctg
    14521 atgattttca ataaattttt acataacacc tgaaccaatt tcttctacta tcttcctgtg
    14581 cattttctca tcaatgggaa aaagccatat aaacacaatg ccgagaataa taagcagcat
    14641 gggaataatg gtactcagca gtcgtatgcc ggtaagcgca ccttcagcct gcatttggga
    14701 ggttaatgtg ttgccaacaa gaagcggttg taggtagcca cacagcgtta gtccaatacc
    14761 ggttataaat cccgcacatg ctgctgctag tttatggctg aaataaaaaa atccataaaa
    14821 aatgccttc
//
LOCUS       DAOSOX010000041        14820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4070_length_14820_cov_7.534981, whole genome shotgun sequence.
ACCESSION   DAOSOX010000041 DAOSOX010000000
VERSION     DAOSOX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_14820_cov_7.534981"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLX22_04660"
     CDS             <1..432
                     /locus_tag="PLX22_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOT19220.1"
                     /translation="HVLIGNEEHLALFLLPNSEKKHDIHSTIQALFNTYANLSAIVIT
                     YREGTSASEVRVGAAAATRNHEVAMKTLEVPHIVDRIGAGDALAAGFIYSLIQQQSLE
                     TAVDTALALCALAHSVHGDNFIVGKADMDAIIQNKSSRLLR"
     gene            511..891
                     /locus_tag="PLX22_04665"
     CDS             511..891
                     /locus_tag="PLX22_04665"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOT19221.1"
                     /translation="MMLQVLDIFFLLFHTLFTLFNMTGWIFARTRKIHLVTMLLTAFS
                     WCVLGIWYGWGYCFCTDWHWQVRNALRKPIVYNSYIQFLLHEITGYTPDAHITDITVA
                     GVFIICLCFSVILNIKDYTAKRKA"
     gene            complement(895..1878)
                     /locus_tag="PLX22_04670"
     CDS             complement(895..1878)
                     /locus_tag="PLX22_04670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOT19222.1"
                     /translation="MIKIAELLPPHPSALWRMVKQCGIEHVVGGMQLYAGWETMPQDF
                     WPWSYNSLVHIKTTYEDAGFKLEVIESRPPMDAIKLGLPERDREIENVIMLIKNMGAL
                     GIPVWCYEWMPVFGWTRTSTTTPIRGGALATSYNHEYMEHAPLTEYGIITEEQLWKNL
                     EYFLKAVIPEAEKAHVKLAMHPDDPPVSPIRGVARIMRSIDNFQKLLDLYPSPVNGIA
                     LCQGNFALMTDNLPQVIHHFGKQGKIFFVHFRNVKGTPYKFTETFHDDGMLDMYQCMK
                     AYHDTGFDGVMRPDHVPTMEGDSNENPAYSSIGRLFAIGYMKGLLEAVSKA"
     gene            2096..2848
                     /locus_tag="PLX22_04675"
     CDS             2096..2848
                     /locus_tag="PLX22_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOT19223.1"
                     /translation="MKLIETLETKLDELIHIHGLVALKGGTEVEDMTHNELLFMKEIA
                     INIPVIVKIGGPEARNDIRYCQSIGIDGILAPMVESEYSLENFVSAMMDIYSNNTLPY
                     LAINVETITAYNNLPAIYSNPYFLYVNQVTVGRSDLSQSMKKAVDDNDVINATAHIVQ
                     CSKIAGKVTSVGGQINPHNAELVQKAIQPDRINTRHLVFDCAKTHNIATSIMVGLEFE
                     LDLYRAFSNVEPLKSTIYQKRIAVTTDRMAIV"
     gene            2975..3121
                     /locus_tag="PLX22_04680"
     CDS             2975..3121
                     /locus_tag="PLX22_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19224.1"
                     /translation="MHSIFVTIMVISSIIVLLIISKIGKNEAPGDVNNNWDGVWKERL
                     KIDN"
     gene            complement(3118..3750)
                     /locus_tag="PLX22_04685"
     CDS             complement(3118..3750)
                     /locus_tag="PLX22_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOT19225.1"
                     /translation="MVKLDNPYSQYRPTWKVFIPMLVMYPLLKVAIQYAYFSFLYQYS
                     IVLLLLIVIAQYCDKGLFTKERIFAFDLPFERKIFYLMVMVIILIPIRNIAIQFTQNS
                     QTLHFYQGQMSSLESMLVMLAIIAPCEELFFRGFVQYNLTFYFGKYGAIVIGALFYSL
                     VGIAAHSLYLAVIFFLIGITIGYIYSTVKSLLQSIVLHVITIIFMYIYPF"
     gene            3915..4889
                     /locus_tag="PLX22_04690"
     CDS             3915..4889
                     /locus_tag="PLX22_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19226.1"
                     /translation="MRRIIVIVCLLHTSLAYPQLLINEIACNVPAGDWVELFYCSEQR
                     LSMDISSLYVTMYYGTNEPLSQSPVTIYSYDRAETPWDDRFVVVHCTKPGIPDECDAT
                     GDTNGNGVLDVYCNNYINSLWNTDCVVAIDSDDEPSNGGIIDFVAYANRDGTLNQSIE
                     KYLKSAIDHNQWVGAYASEECMVDIGQKGILEYQSIIRKNGVDTNSADDFVITSYQTP
                     GRPNILPINHSKSEFIKIKRTIVISKKNMQPLNLFAIQPCKFRIRLFSSVGYKIYESP
                     LINALPGVQLLDWTNTIKHLTCGLYLGIAEAQDNANRESKKFYCIVVE"
     gene            4889..6130
                     /locus_tag="PLX22_04695"
     CDS             4889..6130
                     /locus_tag="PLX22_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19227.1"
                     /translation="MGSLLLTITMFIGAFENAYKVALPLHVQDPLLGKLYNNYPAFVC
                     SLSYANPFGFPQLKETNTNVAYKGKKYDGGIRWHYFGIPEYREDTLSTGLDWAIAHWI
                     KVGCRVHDYILSIKTQEFTTTKHSVDYGLLATVTPFQRLSCTLFTNNIQHYGDDEYIT
                     PTTIFSCDADIFDGCGIEYTLLYNGDIQQCIWIKGYITRHVSVLVGYSKELELSTAGV
                     NVAWGNILLYYMLQHHSFLGNTHRFGITYAGAGYVFPAHSEYKQNTAIKLNIQTCSQQ
                     ELIALGIAPDVLCQRIIKYREMFGAVSTRSLYQLGFTTQQIRQLQEVTCNYCDDDKDE
                     KKDYHKKYQGRTYISKEEQNKKIKRLFQSMVAADIPSDVAIVLAEEYQKAGIPGILRS
                     PLYRSLDTYQQKMVRAACGIQ"
     gene            6115..7671
                     /locus_tag="PLX22_04700"
     CDS             6115..7671
                     /locus_tag="PLX22_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19228.1"
                     /translation="MRNTIVVVLSMVYTVVFIHAVMALSTISVEYREDAMDYEADTQR
                     YLRSSISVTTDYAKVYATLIKTNNGKTTATWAFSNTGEYAAIVAGYYFIHNASGLLLG
                     KPAVYNPDPYSFPVRSSETGISLCKSGNPDYAMHGIVASLYDRLNKPQVRLRIGASLQ
                     ERFMRSDDVEGKLYPYSIGGLLSHDSRDGLYSEPVNIITYFANVTLQPFEYVVLQGCY
                     YHTGLYHENTKIVFDKDNENATAIQSFGGYSLYGRYQDNILTVFTEWAISQKDVKGNS
                     TSTSQYNNAHLYGVTVKKKGIQLCSIIQKTERYFYAPFSSSFGGNAPRDIYYYSAQIK
                     PIRWMLLSWTYMDQNYLVPSTYYAEYPHKRLHCIRTQFTINAFKIQTDYRFMTFYKAG
                     AEQRDSRYQTGMEWMVAKKVSLNMKYGLYTGEESAWYMRSGYTIQWGSLQQAMSVVYV
                     NSNGRKVYCAMLPLANSNILTEVCSSTAVYLAMRVGYRSKLCQLSLRLLSELSHIKNA
                     KGEFGATAWF"
     gene            7731..8534
                     /gene="larE"
                     /locus_tag="PLX22_04705"
     CDS             7731..8534
                     /gene="larE"
                     /locus_tag="PLX22_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOT19229.1"
                     /translation="MDEKKIEQVHQILKKLQPYIVAYSGGNDSTLLVAISMRWNLDFC
                     AVHCISEFTIPGETEYARYFAQHHRIPYHEITVSVMNNSQIIANTEMRCYHCKHEIFK
                     NMVAYAHANGFINVIDASNASDSNDYRPGKKALAELGILSPFVEAGITKDDIYSALAD
                     IQIPHKQFSNSCLATRIPYHTSITNIMLDSIAKAETFIAMLGFTAVRVRYHYPIARIE
                     IPEEEIAYFMDATLRNKVVAFIKELGFLYVTVDCEGFSSGNLNRMLPHE"
     gene            8527..9207
                     /locus_tag="PLX22_04710"
     CDS             8527..9207
                     /locus_tag="PLX22_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19230.1"
                     /translation="MSDILAAFVARNTNEIQTNLDNIQECPFPNMMPLIINDGVEIES
                     ASELADTGIHFVIHEEPLGYGGALVSALSYARDFSLHYLFIMPVEAFTGWQAIATYVQ
                     HFETSDILTGNRLPSSCSAPEGFYGEIVTCLNNGTGLHIVDPLSPVKGIAMKHCDSFE
                     ITEFPDTAFLQLLIQARHFHISIKEFQCSYPESLITSQMQDVEDLPNIKDFIAGELYL
                     YPYIQKGH"
     gene            9207..10286
                     /locus_tag="PLX22_04715"
     CDS             9207..10286
                     /locus_tag="PLX22_04715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT19231.1"
                     /translation="MTVQRIGLIIVLLLNVGLCCSAPEHRLPEQYSGLPSESSVLFVG
                     DMMFEWAVKDTMSKKGVDYPLQNIQSFLHSFDFVMANLETPLTNNSFGDSSFDKKYIF
                     KTTMDIAPLLHKAGIHAVTIANNHILDYGVGGLTDTLVALSSCNILYAGAGINETTAS
                     QPIFCKLGTMQMVIICYTQICSKDMIARDNPGVNFFELSKAIQDIQRYRFADFIVINL
                     HWGNEYFYYPSSKQIGIAHALIDAGADVIVGHHPHVYQGIEIYKNKPIIYSLGNFLFG
                     SMHEGINDTIACALYIAPGGKITRIRVYAIKGKFDDQIIQPEVLGADDATHLLHHFFQ
                     ISKPLDGNFVNRAYITNNYIEYNFR"
     gene            complement(10287..11228)
                     /locus_tag="PLX22_04720"
     CDS             complement(10287..11228)
                     /locus_tag="PLX22_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT19232.1"
                     /translation="MSYTAYCFHPHYLNHTTGDSHPESPKRLEQLDKYIRNNNNYSSL
                     LYVQPRKADPKVLTYAHDINYIQSIANAKPNGITCIDGYDTVICDETYDVSLWAVGGI
                     LEVCDYIMSGEAHNGFCAIRPPGHHAEIAQAMGFCIFNNVAIAARYLQYTYNIRKIAI
                     IDWDAHHGNGTQHIFEADPSVFYISLHQFPFYPGTGSVDEIGIGPGYGYTLNIPMNPG
                     SNDKTYKSAFTEIIIPALDHFQPEFILISAGFDAHKSDPLSSLNLSSSAYYMFTRMLL
                     DIAHKYSNKRVIVVLEGGYEINSMVESVDFVLKALME"
     gene            complement(11324..>11524)
                     /locus_tag="PLX22_04725"
     CDS             complement(11324..>11524)
                     /locus_tag="PLX22_04725"
                     /inference="COORDINATES: protein motif:HMM:NF021902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT19233.1"
                     /translation="YEVLTPPVGRNVLETIRQIHAFQLVRESKGTQATPSGWRPGKDT
                     LKPAPDLVGKVWEVWSVDKAFD"
     assembly_gap    11525..11624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11625..12043)
                     /locus_tag="PLX22_04730"
     CDS             complement(<11625..12043)
                     /locus_tag="PLX22_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT19234.1"
                     /translation="MATITEYITIATKGQGDIIDITMDAQAIVTNNTINNGLLCLFVP
                     GSTAAITTIEYEPGLQKDINIFLETIIPRNAHYNHHDTWHDDNGSSHLRAAMIGASIT
                     VPIVQGKLTLGTWQQIVLIECDTRKRDRKIVAQIVYS"
     gene            complement(12051..12542)
                     /locus_tag="PLX22_04735"
     CDS             complement(12051..12542)
                     /locus_tag="PLX22_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19235.1"
                     /translation="MKKLSPKSQKWLKAFHILCVSLWVGGAITVSGKQFFIQAKTDGE
                     LYGIVSTMDYVDLFIIIPGALGCFVTGLMYSTLTPWGWFKYRWIIVKWIICLYGIIFG
                     TYFLGPWLGKLVHIAQTQGLASYDNPQFNSTVILLMIFGTFQAATLIVACFISSLKPW
                     GKR"
     gene            13044..14102
                     /locus_tag="PLX22_04740"
     CDS             13044..14102
                     /locus_tag="PLX22_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOT19236.1"
                     /translation="MLERLLNIRLFNNTVKEYCVSLLIFIVGIIAIYIFKTIIIRLLK
                     SWARKTTTELDDIAVEAFEKKFIPLIYICSFYVSIIQLTLHPSLKKTLDVIVLILMTI
                     FGVMFVLNGIKIFISAIIQKEGNVTKQKALRALNPLIQILVWSFAIFILLENLGIKLT
                     PLIAGLGIGGLAVALAAQNILGDLFAYFVMLFDRPFEIEDFIIIDKDFMGTVEKIGIK
                     SSKIRGIGGEQIIISNTNLANARVRNYKRMNRRRVVFTIGVVYETGLEDLTLIPEIIK
                     EIIQNIDGTTFDRAHFFSYGDFSLNFEIVYYVNSNDYNVYMDIQQEINLKIKDEFEKH
                     NIEFAYPTSLIYVTKSKM"
     gene            14148..14294
                     /locus_tag="PLX22_04745"
     CDS             14148..14294
                     /locus_tag="PLX22_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19237.1"
                     /translation="MRLVIISNRLPVTVSEKNGRLIIKESVGGLSTGLKAYIDFIKNS
                     SSEN"
BASE COUNT         4750 a         2530 c         2933 g         4507 t
ORIGIN      
        1 catgtgttga tagggaatga agagcatctt gcactttttt tgttgcccaa ttctgagaaa
       61 aaacacgata tccatagcac catacaggca ctctttaata cgtatgcaaa tctttctgcg
      121 attgtgataa cctatcgcga aggtacttcg gcatctgaag taagggtggg cgcagcagca
      181 gcaacacgta accatgaggt ggcgatgaaa acattggagg taccacatat cgttgaccgt
      241 attggtgcag gtgatgctct cgctgcaggg tttatctatt cattgataca acagcaatcg
      301 cttgaaacag cagttgatac tgcccttgcc ttgtgtgccc tggcgcatag tgttcatggt
      361 gataatttta ttgttggtaa agcagatatg gacgcaatca tacagaataa aagcagccga
      421 ttgctgcgat agataatgag acaatgtgct attacattgc caatccgcac tctatttacc
      481 atgatgggat gatatacatg gttatgcact atgatgcttc aggtacttga tatatttttt
      541 ttacttttcc ataccctgtt tacactcttc aacatgacag ggtggatttt tgcaaggaca
      601 cggaagatac accttgtgac gatgcttcta accgcttttt catggtgtgt actgggtata
      661 tggtatggct ggggctattg cttttgtacc gactggcact ggcaggtacg gaatgcactg
      721 agaaaaccta ttgtatataa ttcatatata cagtttttac tccatgaaat aacaggctat
      781 actcccgatg cgcacataac tgatatcacc gtagccggtg tttttattat ttgtttatgc
      841 ttttcagtaa tcctcaatat caaagactac acagccaaaa gaaaagcata aacattatgc
      901 cttgctgaca gcttccagaa gtcctttcat gtacccaata gcaaaaagcc tccctatgga
      961 tgaatatgct ggattttcgt tgctatctcc ttccatggtg gggacatggt cagggcgcat
     1021 gacaccgtca aagcctgtat catggtatgc tttcatgcat tggtacatat cgagcatgcc
     1081 atcatcatga aaggtttcgg taaatttata gggagtacct ttcacatttc tgaagtgcac
     1141 aaagaatatc ttaccctgtt tgccaaagtg atgtataact tgaggaaggt tgtcggtcat
     1201 gagagcaaaa tttccctgac acagtgcaat accattgacc gggcttggat aaaggtcaag
     1261 aagcttttga aaattatcaa tagagcgcat aatgcgggca acgcccctaa tgggcgaaac
     1321 agggggatca tcaggatgca tggcaagttt aacatgcgct ttttcagctt cagggatgac
     1381 cgcctttaaa aaatattcta aatttttcca taactgctct tcggtgataa tgccatattc
     1441 cgttagcggt gcatgctcca tatattcgtg attgtatgaa gtagccagcg cgccgccgcg
     1501 tataggggtt gtggtggaag tacgtgtcca gccaaaaaca ggcatccatt cgtaacacca
     1561 caccggaatt cccagtgcac ccatattttt tataagcatg atgacattct caatctccct
     1621 gtccctttcg ggcaatccta attttatagc atccattggt gggcgcgact caatgacttc
     1681 aagcttaaaa ccagcatctt cataggtggt ttttatgtgg actaacgagt tatagctcca
     1741 tggccagaaa tcctggggca ttgtttccca gcccgcataa agttgcatgc cacctacaac
     1801 gtgctcaatg ccacattgtt taaccatacg ccacagtgct gatgggtgag ggggtaataa
     1861 ttcagcaatt tttatcatat acgattctcc ttgcataaat gaattgtaca actaatcatc
     1921 atatggtctg atgataatat attaaaagca tcaatcgatg tcaaggatgt tttatatatt
     1981 tgttcataaa taatgctcat accggtgaaa atgatacatg aaaaaagaaa ccgtgccgat
     2041 agtatgattg gatagtaagt tgccttcatc ttgcttatga aaggagaatg aaattatgaa
     2101 actcattgaa acattagaaa caaaactgga tgaattaata catattcatg ggcttgtagc
     2161 attgaaaggt ggtacagaag tagaagacat gacacataat gaactgttgt ttatgaagga
     2221 gattgctatc aatataccgg ttatagtcaa aataggagga cctgaggcac ggaatgatat
     2281 ccgctactgt caatcaattg gtattgatgg aattttagca ccaatggttg aatcagaata
     2341 ttcacttgaa aattttgttt cggccatgat ggatatttat agtaataata ctttaccata
     2401 cttagcaata aatgtggaaa ctatcactgc atataacaat ctgccagcaa tctatagtaa
     2461 tccatacttt ctttatgtta accaggtcac tgttgggaga agtgatcttt cacaatcaat
     2521 gaagaaggca gttgatgaca atgatgttat caatgcaaca gcacatattg ttcaatgttc
     2581 aaagatagcc gggaaggtaa cctctgttgg cggtcagatt aatccacaca atgccgaact
     2641 ggttcagaag gctattcagc cagatagaat aaatacacgc catttagtct ttgattgcgc
     2701 aaaaacacac aatatagcta catcaattat ggtaggacta gaatttgaat tggatctgta
     2761 tagagctttt tcaaatgtcg aaccattaaa aagtactatc taccagaaaa ggatagcggt
     2821 taccactgac agaatggcga ttgtgtagta taacacaaca acggtgctac tataacaaaa
     2881 attttataaa aagcttgacg aaaaacctta tttttaacga tatccttcta ctattgatac
     2941 tacttacagc tattgcctaa aaaggagtgt tgctatgcat tcaatatttg tcactataat
     3001 ggttatttca tcgattatag tgttattgat tatatcaaag attggtaaaa atgaagcacc
     3061 gggtgatgtt aacaataact gggacggtgt atggaaggaa cgcttaaaga tagataatta
     3121 aaaaggatag atatacataa aaataatggt tatgacatga agaactatcg attgcagcag
     3181 tgattttacc gtgctgtaga tatatcctat tgttatacca atgaggaaga agatgactgc
     3241 aaggtacagt gaatgtgcgg ctatccctac caaactatag aataatgcgc cgataactat
     3301 cgccccatat ttcccaaaat aaaaggtaag gttgtattgt acaaatcccc tgaaaaaaag
     3361 ctcttcacat ggtgcaataa tagctaacat gacaagcatt gactcaagcg atgacatctg
     3421 cccctgataa aagtgtaacg tttgagagtt ttgtgtaaat tgtatggcta tattacggat
     3481 agggatcagg ataattacca tgaccatgag gtaaaatatc tttcgttcaa aaggaaggtc
     3541 aaaggcaaaa atccgctctt ttgtaaaaag tcctttatca caatattgtg caatgacaat
     3601 taacaataaa agcacaatgc tatactgata taaaaaagaa aaatacgcat actgtattgc
     3661 tactttcaat aacgggtaca tcacaagcat tggaataaaa actttccatg tggggcgata
     3721 ctgtgaataa ggattatcaa gctttaccat ggcaaatact tttccattta aatttagttg
     3781 gtcaagcaat taattgatgc attggcatat tttaaaaaat ttttataaaa aaacaaaaaa
     3841 attcccccat tcttttaatt ttttaacagt ccggcgtatt aataaacaaa ccttacaaca
     3901 cggagatgct ttgtatgcgt cggattattg ttattgtgtg tttgcttcac acatcattag
     3961 cctatccgca acttctcatt aatgaaatag cctgtaatgt accagcgggt gattgggttg
     4021 agctatttta ctgctctgaa caaaggctat cgatggatat ttcatcgctg tatgtaacca
     4081 tgtattatgg aaccaatgaa cctctttcac aatctccggt tacaatctat agctatgata
     4141 gggcagaaac accatgggat gacaggtttg ttgttgtgca ttgtacaaag cctggaatac
     4201 cagatgagtg tgatgctact ggagatacca atggcaatgg tgtgcttgat gtttattgca
     4261 ataactatat caatagctta tggaataccg attgtgtggt ggcaatagat agtgatgatg
     4321 agccgtcaaa tggcggaata attgattttg tggcgtacgc caacagagac ggtactctta
     4381 accagtcaat tgaaaaatac ctcaaaagcg ctattgacca taatcaatgg gtgggtgctt
     4441 atgccagtga agaatgcatg gttgatattg gtcaaaaagg aattttagaa tatcagagta
     4501 ttattagaaa aaatggtgta gataccaata gcgctgatga ttttgtcatt acctcgtatc
     4561 aaacgccagg gcgccccaat attttgccaa taaatcattc aaagtccgaa tttataaaaa
     4621 ttaagcgcac gatagtcatc tctaaaaaaa atatgcagcc gcttaatctt tttgctatcc
     4681 agccatgcaa gttcaggata cgcctgtttt cctctgttgg ctacaaaata tacgaaagtc
     4741 cactgattaa cgcattgcct ggtgtccagt tacttgattg gacaaacact atcaagcatc
     4801 ttacctgtgg tttatatctg ggtattgctg aggctcagga caatgccaac cgcgagagta
     4861 aaaaatttta ctgtatcgtg gttgagtaat gggttcattg ctgctgacaa taaccatgtt
     4921 cataggcgca tttgaaaatg catataaagt agcattaccg ttacatgtgc aggacccatt
     4981 attggggaaa ttgtacaaca attatcctgc gtttgtgtgc agccttagtt atgcaaatcc
     5041 ttttggtttt cctcagctta aggaaacaaa tacaaatgtt gcctataagg gtaaaaaata
     5101 tgatggcggc atacggtggc attattttgg cattcctgaa tatcgtgaag atacactgtc
     5161 aacagggctt gattgggcga tagctcactg gataaaggtt ggctgcaggg tacatgacta
     5221 tatcctttca ataaaaactc aagagtttac cacaacaaaa catagtgttg attatggatt
     5281 gctggctacg gtaacaccct ttcaacgatt gtcgtgcacc ctatttacca acaatataca
     5341 gcattacggc gatgatgagt atattacgcc aactaccata ttttcatgcg atgctgatat
     5401 ttttgatgga tgtggtattg aatatacgct tttgtacaat ggggatatac agcaatgtat
     5461 atggataaaa ggatatataa ccaggcatgt aagcgtactg gttggataca gtaaggaact
     5521 ggaattatct acagccggtg taaatgttgc atggggcaat atccttcttt actatatgct
     5581 tcagcatcat tcatttctgg gaaacacgca tagatttggg ataacctatg caggtgcggg
     5641 atatgttttc ccggctcata gtgaatataa acaaaatact gcaataaagc tcaatataca
     5701 gacatgcagc caacaggagc ttattgctct gggtattgca cctgatgtct tgtgtcagcg
     5761 tattatcaaa tatagagaga tgtttggagc tgtcagcaca agatcattat atcagctggg
     5821 atttacaaca caacagatac gtcaattgca ggaggttacc tgtaattatt gtgatgatga
     5881 caaggacgaa aaaaaggatt accataagaa atatcaaggc agaacatata tatcaaaaga
     5941 agagcagaat aaaaaaataa aaaggctctt tcagtctatg gtagctgccg atataccgtc
     6001 ggatgttgcc atagtgctgg ctgaggaata tcaaaaggcg ggtataccgg ggatactgcg
     6061 ttcaccatta tatagaagtc ttgataccta tcagcaaaaa atggtcaggg ccgcatgcgg
     6121 aatacaatag ttgtagtttt aagcatggta tatacggtag tttttattca tgcagtaatg
     6181 gcattgagca ctatatcagt tgaatataga gaggatgcca tggattatga agctgatacg
     6241 caacgatatc tacgttcctc aataagtgtc acaacagatt atgcaaaggt ttatgcaaca
     6301 ttgataaaaa caaataatgg caaaacaacg gcaacctggg cttttagcaa tacaggggaa
     6361 tatgcggcga tagtagctgg atattatttt atccataatg catcagggct attgctgggt
     6421 aagcctgctg tttataatcc cgatccatac tcattccctg tacggagcag cgaaacggga
     6481 atatcactat gtaaaagcgg aaatcctgac tacgcgatgc atgggatagt tgcatcgctc
     6541 tatgatagat taaacaaacc gcaggtacga ttgcgtattg gagcatcatt gcaggagaga
     6601 tttatgcgat ctgatgatgt tgagggtaag ctgtatccct atagtattgg tggtttgctt
     6661 tcgcatgaca gcagggatgg attatattcc gaaccagtta acattataac ctattttgct
     6721 aatgtaacgt tgcagccctt tgaatatgtt gttttgcagg gatgctatta ccataccgga
     6781 ttatatcatg agaatacaaa aatagtattt gataaagata atgagaatgc aacagcaata
     6841 cagtcgtttg gagggtatag cctctatgga agatatcaag ataacatact aactgtattt
     6901 actgagtggg ctatctcaca aaaggatgta aaaggcaata gcacttcaac atcacaatac
     6961 aacaatgctc acctgtatgg ggttaccgtt aaaaagaaag ggatacaatt gtgcagcata
     7021 atacaaaaaa cagaacgata tttttatgct ccattcagca gttcatttgg cggtaatgcg
     7081 ccgcgtgata tttattatta ttcagctcag ataaaaccaa tcaggtggat gttgctttca
     7141 tggacataca tggaccagaa ctatcttgta ccatcaacct attatgctga atatcctcat
     7201 aaacggctcc attgtatacg aactcagttt accataaacg cctttaagat acaaacggat
     7261 tatcgcttta tgacatttta caaagctggt gcagagcagc gggattcgcg atatcaaaca
     7321 ggaatggaat ggatggttgc caaaaaagta tcactgaata tgaaatacgg gctatacacg
     7381 ggagaggaat cagcatggta tatgcgatca ggctatacta tacagtgggg ttctttacag
     7441 caggcgatga gtgttgtcta tgtaaacagt aatgggagga aggtgtactg tgcaatgctt
     7501 cctttagcaa acagcaatat actcaccgag gtttgctcca gtacagccgt ttatctggct
     7561 atgcgggttg gctatcgcag caagttatgt cagctgtcat tgaggttatt atcggaacta
     7621 tcgcacataa aaaatgctaa aggtgaattt ggtgcaactg catggtttta gataaattga
     7681 ttctatagag tagttgcact aaaaggtatt tgtggtataa tggttaccgt atggatgaaa
     7741 aaaagattga acaagtacat caaatactga aaaaactcca gccgtatatc gtggcatatt
     7801 caggaggtaa tgattcaacg ttacttgttg caatatccat gcgctggaat cttgattttt
     7861 gtgctgtgca ttgcatcagc gaatttacca ttccgggtga aacagaatat gcccgttatt
     7921 ttgcacagca ccatcgtatc ccctatcacg aaattacagt atcggtaatg aacaatagcc
     7981 agattatagc aaatactgaa atgcggtgct accactgtaa acatgaaatt tttaagaata
     8041 tggttgcgta tgcacacgct aatggtttta taaatgtcat tgatgcaagc aatgcttctg
     8101 atagtaacga ttataggccc ggaaaaaagg cgctggcaga attagggata ctatcgccgt
     8161 ttgttgaggc tggcataaca aaagatgata tttatagtgc tctggcagat atacagatac
     8221 cgcacaaaca attttccaac tcatgtttag ccacgcgcat accgtaccat accagcataa
     8281 caaatattat gcttgatagt attgcaaaag cagaaacttt tattgccatg ttgggattta
     8341 cagcggtacg ggtgcggtat cattatccca ttgcacgtat tgagatacca gaagaagaga
     8401 ttgcatattt tatggatgca accctccgta ataaagttgt tgcatttata aaggagcttg
     8461 gttttttata cgtaacggtt gattgtgaag gtttttcatc aggaaattta aacaggatgt
     8521 taccacatga gtgatatatt agcagctttt gttgcccgta atactaatga aatacaaaca
     8581 aatctggaca atatacagga atgtccattc cccaacatga tgccgcttat catcaatgat
     8641 ggtgtggaga ttgaatctgc atctgaactt gctgataccg gaatacattt tgtcattcat
     8701 gaagaaccgt taggatatgg aggagcactg gtcagtgcac tatcgtatgc acgggatttt
     8761 tcattacact acctatttat aatgcctgtt gaagcgttta ccggctggca ggcaatagca
     8821 acgtatgtcc agcattttga aacatcagat attcttacag ggaacagact accctcctca
     8881 tgcagtgccc ctgaagggtt ttatggggaa attgttacct gcctgaataa tggaacagga
     8941 ttgcatatcg ttgatcccct gtcacctgtt aaagggattg ccatgaagca ttgcgatagt
     9001 tttgaaataa cagaatttcc tgatactgct tttttacaat tattaatcca ggcacgtcac
     9061 ttccatatat caataaagga gtttcaatgc tcgtatccag aatcgcttat tacatcgcag
     9121 atgcaggatg ttgaagactt gcccaatata aaagatttta tagctggtga gctttatctg
     9181 tatccgtata tccagaaagg gcattaatga cagtgcaaag aattggattg attatagtac
     9241 tattgttgaa tgttggatta tgctgctcag ctcccgagca tcgcctgcca gagcaatatt
     9301 ctggattacc ttctgaaagc agtgttcttt ttgttggtga tatgatgttt gaatgggcag
     9361 ttaaagatac catgagcaaa aagggtgtgg attatcccct gcaaaacata caaagctttt
     9421 tacattcatt tgactttgtc atggcaaatc tggaaacacc tttgaccaat aatagttttg
     9481 gcgatagttc ctttgataag aagtatatct ttaaaacaac gatggatatt gcccccctat
     9541 tacataaagc aggcatacac gctgtaacca tagcaaataa tcatatattg gattatggcg
     9601 ttggtggatt aaccgacaca cttgttgctc tttcttcctg taacatattg tatgcaggag
     9661 caggtattaa tgaaacaaca gcttctcagc caatattttg taaattgggt accatgcaga
     9721 tggttattat atgctatacg caaatctgtt caaaagacat gattgccagg gataaccccg
     9781 gtgtaaattt ttttgaatta tcaaaggcta ttcaggatat tcaacggtat cgttttgcag
     9841 atttcattgt tatcaatctg cactggggta atgaatattt ttactatcct tcttcaaaac
     9901 agattggaat agcccatgcg cttatagatg ctggagcaga tgttattgtt ggtcatcatc
     9961 cgcatgttta tcagggtatt gaaatatata aaaacaagcc cattatttat tcattgggga
    10021 attttttatt tggatcgatg catgaaggaa tcaatgatac cattgcatgc gctttatata
    10081 ttgcacctgg gggaaaaatt accaggatac gtgtctatgc aattaaaggt aaatttgatg
    10141 atcagattat acaacctgag gtgctgggtg cagatgatgc aacacacctc ttgcaccatt
    10201 tttttcaaat atcaaagccg ctggatggca attttgtaaa cagagcttat attactaaca
    10261 attatatcga atataatttt cgatgattat tccatcagtg ctttcagcac aaagtcaaca
    10321 ctttctacca ttgaattgat ttcataacca ccttcaagca ctacaatcac tcttttattg
    10381 gaatatttgt gtgcaatatc taacagcatg cgtgtgaaca tgtagtatgc actacttgat
    10441 aaatttaatg aggataatgg atcgctttta tgggcatcaa aaccggctga tattaatata
    10501 aactctggtt gaaaatggtc aagtgcaggg attatgattt cagtgaatgc acttttatag
    10561 gttttatcat tgcttcccgg attcattggt atattaagag tatatccata tccaggtcca
    10621 atgccaattt catcaacact accggtcccg ggataaaaag ggaattgatg gaggctgatg
    10681 taaaaaacag aaggatcggc ttcaaaaata tgctgtgtac cgttgccgtg atgtgcatcc
    10741 caatcaataa tagcaatttt tcggatattg tatgtatatt gtaaataacg agcagctatt
    10801 gctacattgt taaatatgca aaaacccata gcctgagcta tctcagcatg atgaccggga
    10861 gggcgtatgg cacaaaatcc attgtgagct tcacctgaca taatatagtc gcatacttct
    10921 aaaatgccgc ccactgccca caatgataca tcatacgttt cgtcgcatat tactgtatcg
    10981 tatccatcaa tacaggttat tccattgggt tttgcattgg ctattgattg aatataatta
    11041 atatcatgag catacgttaa cacttttggg tctgcttttc gtggctggac atacagcaat
    11101 gatgaataat tgttattatt acggatatat ttatctaatt gttcaaggcg tttaggggat
    11161 tcagggtgag aatcccctgt tgtatggttt aaataatgtg gatgaaagca atatgctgta
    11221 tagctcataa ttattaaatg tttgatagtc gaattataat gtacacataa cttaataata
    11281 ttgtaatata atattaaaaa aaatcaagta ttttttaaaa attttagtca aaagctttat
    11341 caacagacca aacttcccac accttaccta ccagatcggg agctggtttt aacgtgtctt
    11401 tgcccggacg ccatcctgaa ggtgttgctt gagttccttt agattcacgg accaattgga
    11461 aagcatggat ttgtcggata gtttccagaa cattacgccc aaccggtggt gttaaaactt
    11521 catannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntatgaa tatacaatct
    11641 gagctactat cttcctatcc ctttttcggg tatcgcattc tatgagtact atctgctgcc
    11701 atgtccccag tgtaagctta ccctgaacta ttgggacagt gattgaagca cctatcattg
    11761 cagctctgag gtgcgaagaa ccattatcat catgccatgt atcgtgatgg ttataatgag
    11821 cgttgcgtgg aatgattgtc tcaagaaaaa tatttatatc tttctgtaag cctggttcgt
    11881 attcaatggt ggtgatagca gcagtggaac ctggcacaaa aaggcataat agcccattgt
    11941 taatagtatt attagtaact atcgcttgcg catccattgt tatatcaatg atatcaccct
    12001 gaccttttgt tgctatggta atgtattcag taatggttgc cataaactgc tcaccttttt
    12061 ccccatggtt taaggcttga tataaagcag gcaactatta atgtagcggc ctgaaaagta
    12121 ccaaaaatca tcaacaagat aaccgtgctg ttgaattgtg ggttgtcata actggcaagc
    12181 ccctgtgttt gtgctatatg aacaagtttg cctaaccatg gaccaagaaa ataagtacca
    12241 aaaataatac catataaaca tattatccat ttaacaataa tccatctgta tttaaaccac
    12301 ccccatggtg ttaatgttga gtacataagt cctgttacaa aacaaccaag tgcgccagga
    12361 atgataataa aaagatcaac ataatccatt gtagatacaa taccatatag ttcaccatca
    12421 gtttttgcct gtataaaaaa ttgcttacct gatactgtga ttgcaccgcc aacccataat
    12481 gaaacacata gtatatggaa agcttttaac catttttgac ttttaggaga tagttttttc
    12541 ataattttac cctgcattaa aagttttgta aattgaaata gttatagtat tagatttaat
    12601 tacgaatctt tacatagaaa tcaagtacaa tattttttgc aataaaacat aagaggctgt
    12661 aatcaatggt tacatacgtt tcatgaccat ggttcaaaga ttacaaaaca tataaggcaa
    12721 tagcgaggtg cgatagttac ttgaaatatg caaaaatgga tagatgaagt ttgaaaggag
    12781 tgccatatct gttgaccttg taattatgta tcacaacata aaacatgaaa aaagtttgac
    12841 aaagtataaa tttattatag atattcaata tggtttataa taaaacgaag tcatgaatat
    12901 tgaataaaag tattataata attatactat atgtataatt cacagatgaa catatctgct
    12961 tgaataattt ttttgcaata aattcttctc atttcataca ataactatta cctttgatta
    13021 catgataatg tgaggatata gctatgcttg aaaggttgct caatataagg ctatttaaca
    13081 atacagtgaa agagtattgt gtaagtttgt taatttttat tgttgggatt atagctattt
    13141 atattttcaa aacaataatt ataagattat taaaatcatg ggcacggaaa acaacaacag
    13201 aattggatga tattgctgtt gaagcatttg aaaaaaaatt tatccccttg atatatatat
    13261 gttcattcta tgtcagtatt atacaattaa cattacatcc ttctttaaag aagacattgg
    13321 atgttattgt attaatactg atgactatat ttggtgtgat gtttgttctc aatgggatta
    13381 aaatttttat ttccgctata atacaaaaag aaggcaatgt aacaaaacaa aaagcattaa
    13441 gagcattgaa tccgttgata cagattcttg tatggtcgtt tgcaatcttt attttattag
    13501 aaaatttagg gattaaactt accccgctga ttgccgggct tggcataggc gggttggctg
    13561 ttgcattggc tgctcaaaat atactgggag atctatttgc atattttgtt atgctctttg
    13621 atcgtccttt tgaaattgaa gattttatca ttattgataa agatttcatg ggtacagtag
    13681 aaaaaatagg tatcaagtcg tcaaagatac ggggtatagg cggtgaacaa ataattattt
    13741 ctaataccaa ccttgccaat gctcgtgttc gcaattataa aagaatgaat agaagaaggg
    13801 ttgtttttac cattggtgtt gtgtatgaga cagggcttga agatttaacg ttaatcccag
    13861 agattataaa agagattatc cagaatattg atggtacaac ctttgataga gcacattttt
    13921 tttcgtatgg tgattttagt ttaaattttg agattgtata ctatgtgaac agcaatgatt
    13981 acaatgtata tatggacata caacaggaaa tcaatcttaa gataaaggat gaatttgaaa
    14041 aacataacat tgaatttgcc tatccaacaa gtcttattta tgtaacaaaa agcaagatgt
    14101 agccgtatgg attattgtat aattaataaa tagataagta ggtgagtatg cgtttagtaa
    14161 ttatttcaaa caggttgcct gttacagttt ctgaaaaaaa tggcaggttg attattaaag
    14221 aaagtgtggg agggctttca accggattaa aagcatatat cgattttata aagaattcat
    14281 cctccgaaaa ttgaatatat aatatatatg gataggatgg gacattagta gggtttgaaa
    14341 atgtcctgat tattgatggt gaaaacggat tccttgttga tccctatgat attgatggat
    14401 gtgccaaaaa agtaattgag attcttaaaa atgaagagct tagaatcagt atggggaaaa
    14461 atggaaaaga acatgtaagg aagaacttcc tcattacaag agaacttatg gatcatctgg
    14521 acatgttaat tgatgtgtta ttgtagatgt atgtgttatg ataatagtaa agaccccctt
    14581 aaaaaataac agtagacatg taaaacttta atgttcattc ctgcaaaatc tcgtaaaaaa
    14641 gacttcaatc attacttatc ataatggtaa gcctggtaaa acgtcaatag caataccatt
    14701 aaaataaaat aataaaagta aaattacttg actattaaaa tatagctata acatatcgtt
    14761 aaagatgtaa tattgtatga taattatatg aggttgacat gaaaacattt ttttaccccg
//
LOCUS       DAOSOX010000042        14686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4122_length_14686_cov_10.148452, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000042 DAOSOX010000000
VERSION     DAOSOX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4122_length_14686_cov_10.148452"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..618
                     /locus_tag="PLX22_04750"
     CDS             337..618
                     /locus_tag="PLX22_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19238.1"
                     /translation="MAKEIDELRKIVQTYENVLKLNEQELANAYQIISMYENIVEYSR
                     TELKLATETAQARESASQLSRMELMQAFEKIQKLEEDNKKLRAKKENAS"
     gene            654..2006
                     /locus_tag="PLX22_04755"
     CDS             654..2006
                     /locus_tag="PLX22_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19239.1"
                     /translation="MLITYAIIFLFIIILVIFVLYFANIYIFPRKLEEIQQLIESGQT
                     KIAIRKLNDIIEKDDRNHYAHYLLALAYEKENNIQYAILEYRQVLKIGRYDEKVNEVN
                     IRNHLASIYKARNAVEEAKKEYLLLTKLDPANFEPFYELGIIYFNAGQIDKAIPYFKK
                     SIANNNKHGASFYYLGQAYYRMQNYPDAKQAFIEAIKVDPHDYKAHYFLGLVLRQLGD
                     YEWAIKEFETAQKSDDIKVKCFLAKGTCYLEREQLPKAVIEFERGLKFAKRGSDTELN
                     LRYFLAEAQEKMRDLHSAISNWEKIVAVNPTFRDVQAKLKSYAEFRQDDRIKDFMIAG
                     LAQFEHTCRKISESMGLTVMDVDIISDTEVEIIATDTEGKWRNTRRTNKIVRILRTTD
                     VVNDKLLRKLHEKLKEKNATRIIIITAGDFTQSAVDFSNTRPIELYGKNDLLRLLKQI
                     "
     gene            2011..3819
                     /locus_tag="PLX22_04760"
     CDS             2011..3819
                     /locus_tag="PLX22_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT19240.1"
                     /translation="MVNKAILLLNQVPIFKHLMADELTALSLLAKPHTVKKGSTFHCN
                     AQSIYIVEKGVFGVQKSSESIYLSRTSYFGQLPFCEKSTGVVRALSDASVLEFANTDL
                     YQFICNNFKALRGYCRILDHYRIPLSQTATTLLKNKACVYTIFSTDKHCGKTTLAISV
                     ATACSYYKRTILLDASYEGISVFDMCGKPLIPPLSQKNPDDVTGYKFIQQRIEKISET
                     MDIANIAYGSKVKINPDIINPLLFYLSFEYSYIVIDCSNSDIHLRDVIMKNSDSIINV
                     INEKSAINHQVVNDTLLTQGQQVYNVVNKHFVKIQKIDQPSYVFKTIPVETNETIFQA
                     VLKNKMYNDELFKNIVSDKRAMVLSHGMYETLYYSGVLESLREEKLFDVIYANGIPYA
                     VLMCYLATQTTEDYKKLLFDYFSDDSLHTFFDIAFPDKYLFKNKPLHNFASAISHIKR
                     IEWNAILPYTQLMHNNTQRFFSTGDAEKIISANLAFSPLFEGISIHGDAYFSAFPLYP
                     LIPESLLRTHINYIGYIAIANPYNPISVKSKIPGLYRKIVDFLPDCYLYACDNTFCCN
                     RLISLTLPDVTSTHDLIKQSLKNTDMFISMIKKMIK"
     gene            3860..4306
                     /locus_tag="PLX22_04765"
     CDS             3860..4306
                     /locus_tag="PLX22_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19241.1"
                     /translation="MRSKATNILQTGVLLTGIIYIIIGLLYGFSPIYFANIFGIEVNP
                     DWYNLIKYDTFIAPLYHFSRIFALLLAIVGISMLLPLFDPLKYRGMIYYHSILFPLVS
                     VPSLLINGIAYKHLPLTICGCLLLIIFLFVGFGLIITKKYAKMGLE"
     gene            4423..4761
                     /locus_tag="PLX22_04770"
     CDS             4423..4761
                     /locus_tag="PLX22_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19242.1"
                     /translation="MNLKTKKVGNIVVVYLQGRLDVHLSADIEKEINKLIKDDPASHL
                     LLNLAGVEYMSSSGLRIFVSTMRILKESNRKLKICSMNNAVKKIFEVVELMDMFEIYE
                     TEEEAIASFK"
     gene            4851..7031
                     /locus_tag="PLX22_04775"
     CDS             4851..7031
                     /locus_tag="PLX22_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19243.1"
                     /translation="MAIPLELSEGIKNFEIYVKHNYFSWPYWIAHYTNRENVRIQSLP
                     FILYNNHCRTWYTINSFLNSGAIRIDPCGMIHPDWAKWSVECMVYSDGAYIKFHDDTC
                     IHQQNERGVIKTSYQHHLFRFLQSVYIAHTNVDEAIVINKINIKKKNQSYFIIAIRPY
                     TTTTLSGINSIELLKNNLVKINDDHACSLIEKPDYILVGNEIIGDIDILQKDDKYRIS
                     SSSGMATMAFAYELKNTDHEYGIRIATGKNTKLSVSTLSVKSALNDYTSYVDSIMKQG
                     IAISIGRHTINEWTDFCKLSTLVHASTMASIKTTQLQRLKQYYYISSTLNRMGYLKQT
                     ELAIDTIKKNIKITDDYEGVLSSSFFCLIVGDCYRTIKDSSFVQNNYSLCKQYAYSIT
                     EFFKKIGDITKENNFKDNPQIAINAIINIAYIISALENVAFMSRSLGLFGEETKIFSD
                     IKRLQNVILKSIFTINSETNGEEEKLIEWTPKYSKPIRKQKIIVKDSLEFSHFVNVND
                     NRIISYSPLLYPYMKDIMQPYIVKNIINAIIHAGKGLPLYNTIISAVDIVNSLSLANA
                     MIYAHDKRAIDSINAIINLCNKQKFLPDFLDPVTGHAVVNDPVSTIAVSLLFQAIRNL
                     CFIDHPERLEIFPVPWEEWFNDCQMNIKNAHSRFGIISFTATSTSNEFVIQFTDIPRF
                     IPHDILVNLPFKAKIKKSDDFIVKYQTEKSWCINGWPSEIRFKR"
     gene            7101..8645
                     /locus_tag="PLX22_04780"
     CDS             7101..8645
                     /locus_tag="PLX22_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19244.1"
                     /translation="MISYLVISLLIISGFVYYLVYIRPKFNPLNKAEQFLKTNQLHDA
                     IIEYKKVIDEYPDNFVVHHRLAELYLTMGEIDQAVIHLEKILEIGKFNYEVDQLSVLK
                     KLAKSYYVRDEVEKAFKMYMDILKNFPADIDSLYHVSFILLSQEEFDIAQRYFERLLK
                     LKNDSYEILFGAGICNYQNMRIQEAIDNFRLALQLKPQSEIAQIALAFAFNRKRDYRQ
                     ALTYITKVAESAQDPDVQYLAKRFQAFLYMRLKRHDEGIKLFQDLLEIARKNANDDAE
                     ALALHDLGYALLKNEQSSLAYDVWNELYRKDRNYKNIQFLVTQLRKEMEADKFVKEES
                     ASITETITAWMEEAFPQNLLWDMCGLKGSKRYNLKDVVVTTRITFDDGKLSSTSDEII
                     QKYIALDTENFRIISNRLVSKVGFKVDQILQTYREADGVDFLAVNPETKDKVLVWVRR
                     WTKTNVGEITLRNFAQAINDMKVKQGILITTADLTDIAKETLKQLSKISVIYPDEIEK
                     LLRGLL"
     gene            8646..9575
                     /locus_tag="PLX22_04785"
     CDS             8646..9575
                     /locus_tag="PLX22_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOT19245.1"
                     /translation="MPFDNFTSTMIPLQSINYINDYYKISKPLSPELIKSIKQNGVLE
                     PVILIEGNGCYIPVCGHNRMLASIQTELANVPAKIINGLSEDDFMAIITLKLYNNLMG
                     TIGKLKSLFILKEYFKINKKQLIAIAHELSVPDALIETTSLKKIIGLPSSLMNYIDSK
                     NIPFKFIMPVLRLSDESIQLVAAIINACQLRVNYFRDIVVMIEDICRNQHYESVLKKI
                     TVLINKENRTDDEIYRNIYLLRYPQYSEYVEKINYLKNKFLSYGLKIEVPEYLEGNEV
                     NIIIQVKKNEVVHPLLQQDKEIRALIQSIIELL"
     gene            complement(9586..10563)
                     /locus_tag="PLX22_04790"
     CDS             complement(9586..10563)
                     /locus_tag="PLX22_04790"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOT19246.1"
                     /translation="MDYFANLEKYLNAHDHFIISTHESPDPDGLGAEIGFKEILLHLG
                     KHAIIINSDPTPTKFNFIDPDNEIIIFDEKMIIDNISNYTIFVLDTNDFNNIGKAYHY
                     LNNIVKDVFIIDHHEGANNKLETNYIKVEASSTSEIICTIMERYNIKPSFKAAQALYA
                     GIVFDTGCFKYPKTSSHTFTIAAQLISSGIKPYEVYQWIYENNTLEVFRLRALMLSSM
                     EVHFGGKLILMELTPEMIKATNAPFSEGEVNINLPLTINGVVASVVVKQDVDGPVKVS
                     MRTKGDLDVAAIAIANGGGGHKNAAGFKSPYNFETTKQKILQEMSAFFK"
     gene            complement(10563..11468)
                     /gene="glyQ"
                     /locus_tag="PLX22_04795"
     CDS             complement(10563..11468)
                     /gene="glyQ"
                     /locus_tag="PLX22_04795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOT19247.1"
                     /translation="MYFQDLIASCNSFWAKQGCIIAQGYDMEVGAGTFNPATFLRVLG
                     PEPWNVAYVEPSRRPTDGRYGDNPNRLQHYYQYQVIMKPSPLDIQDLYLDSLRNLGIK
                     LEEHDVRFVEDDWESPTLGASGLGWEVWLDGMEITQFTYFQQCGSIELDPIPVEITYG
                     LERICMYIQGVESVFDIQWNDTLTYRDVHHRSEFEFSHYNFSIADTALLFQLFDLYEK
                     ECLKILDYEPVQVILPAYDYVLKCSHVFNLLDARGAISVTERVGYITRVRNIARRCAE
                     RYVAIRESMGFPLCKYAKYASPEKA"
     gene            complement(11556..13076)
                     /locus_tag="PLX22_04800"
     CDS             complement(11556..13076)
                     /locus_tag="PLX22_04800"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT19248.1"
                     /translation="MEGTQQKSHYFCKPVDYKEALTRGEDVYLQFSLLTPEVEALLIR
                     VLSIYLSQLDLLYLKDMFINILKELVNNAIKANAKRLYFKRKNLDINKKEEYRNGMET
                     FKEDVFVGNPEFLEELGKSNFIVRVFFSITGDIHKIHVINNVPIIPEEMNKINARIKK
                     AYTYHGISDAFDDILDDSEGAGLGLIITLILMKNAGFPPEAFSMSTNGKITSATINID
                     ESFRNMDLKVKMAQEIINEVESLPSFPQNILEIQRLCANPESTIKAIADSIKRDPGLV
                     TAILKLANSAGYITSKRVETIEEAVKIIGTKGINTLLVASGVQKIIDSRYKKFETIWN
                     NSYKTAFYAQWIAMKYRKERLSEFAYLASLLSDIGLMVLLSIKPDEIQKIREIVGLKQ
                     LDNISILEEISLGVSHSTLGSLICQKWKFNDALVKAIEFHHRPHLAPSQYRDLIYIVY
                     LAYVFTSIESKNFRFEIADEDCLDYFKLNNKTNFDELHQLIKAKFEEYSQNNKITN"
     assembly_gap    13266..13365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13366..14513
                     /locus_tag="PLX22_04805"
     CDS             <13366..14513
                     /locus_tag="PLX22_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT19249.1"
                     /translation="DYTNTIRNCCASEVHMSVVYFTDLHMSSKENIVSKIKKLFYKLS
                     ANEIINEKDFVAIKTHFGEYGNIAYTPPVIIHTFVDCVKDLKANPFVTDSTTLYRGSR
                     SNAVDHLKNAAMNGFSLVSCGAPVIIADGLRGNDFKELPATGKWYSTIKVASAIHDAD
                     SMVVVSHVKGHELYGFGGAIKNIAMGCAPPSMKQSLHSDLKPKVKQTLCTSCGACIKR
                     CPAFAISFDANKKAFINSDKCFGCGECTVICVYNAIPVLWKTDHTTLYERTAEYVNAF
                     MKHKENKVLFFNFIMNVSPECDCCYWNDIPIVPNIGIVASTDPIAVDKASIDLINKAT
                     PMPDSRIAHKDSSKDNIEALYPVKWNCLFEYATSIGAGNMEYELVTI"
BASE COUNT         4999 a         2243 c         2543 g         4801 t
ORIGIN      
        1 acccctgacc tcttgaatgc cattcaagcg ctctcccaac tgagctacgc cccctgattg
       61 ttgtgcagtg taactttatc atttatttaa aggaataatt atcagaaaat ttgtcaatat
      121 attttaaata tattttaaat atattttaaa tatattttaa tagtacttat aaaaatttac
      181 tatctgtggt gatttattct tgcaaaaatg gtgattgctg tgatactata gctatacgat
      241 atgcatgata gtggggctga actattacaa cataaaatat tttgaggtta ggttatgtta
      301 tcagaaattg ataagcttaa aaatgaaaat gaaattatgg caaaagagat tgatgagctg
      361 cggaagatag tgcagacata tgaaaatgtt ttaaaactca atgaacagga attggccaat
      421 gcctatcaaa tcatatcaat gtatgaaaat attgtagaat attcccgaac cgaattaaag
      481 ttggcaactg aaacggctca ggcacgtgaa agtgcatcac agttaagcag gatggaattg
      541 atgcaggcat ttgaaaagat tcaaaagctt gaagaagata ataaaaaact cagagccaaa
      601 aaagaaaatg catcctgata aaattaagat gaaggtaatt gggtagtaaa aacatgttga
      661 ttacgtatgc tataatattt ttgttcatta taattctggt aatatttgtt ctttattttg
      721 ctaatattta tattttcccg cgtaagctcg aagaaatcca gcaacttatt gagtcagggc
      781 agacaaaaat agccatcaga aaattaaatg atattattga aaaagatgat cgtaatcatt
      841 atgctcatta tttgctggcg ttagcatatg aaaaagaaaa taatattcag tatgcaattc
      901 ttgaatatcg gcaggtttta aaaattggaa gatatgatga aaaggttaat gaagtcaaca
      961 tacgcaatca tttagcaagt atttataagg cgcgaaatgc agtagaagaa gcaaagaagg
     1021 aataccttct tttaacaaaa ttggatcctg caaactttga acctttttat gaacttggta
     1081 tcatttattt taatgcagga cagatagata aagctatccc gtattttaaa aaaagcattg
     1141 caaataacaa caagcatgga gcttcatttt actatttggg acaggcatac tatagaatgc
     1201 aaaactatcc tgatgcaaag caagctttta ttgaagccat taaggttgat ccacatgatt
     1261 ataaagctca ttacttttta ggtttggttt tgcgacagtt aggcgattat gaatgggcaa
     1321 ttaaagaatt tgagacagca cagaaaagcg atgacattaa ggttaaatgt ttccttgcta
     1381 aagggacatg ttatttagag cgtgagcaat tgccaaaggc agtgattgaa tttgaacgtg
     1441 gtttaaaatt tgctaaaaga ggtagcgata ccgagcttaa tttgcggtat tttctggctg
     1501 aagctcagga aaaaatgcga gatttacact cagccatatc aaactgggaa aagatagttg
     1561 cggtcaatcc tacgtttcgt gatgtacagg caaagcttaa atcgtatgca gaatttagac
     1621 aggatgatcg tattaaagat tttatgattg cagggctggc acagtttgag catacctgcc
     1681 gtaagatatc tgaatcaatg ggacttactg ttatggatgt agatatcatc agtgatacag
     1741 aagttgaaat catagccacg gatactgaag gaaaatggcg taatacacgc cgtactaata
     1801 aaattgtacg aatacttaga actaccgatg ttgtaaatga taaattgctg cgcaaactgc
     1861 atgaaaagct taaagaaaag aatgcaacac gtattatcat tattactgca ggtgatttta
     1921 cacaaagtgc tgttgatttt tcaaatacca ggcctataga gttatatggg aaaaatgatc
     1981 ttttacgatt attaaaacaa atataattgt atggtaaaca aagcaatact tttattgaat
     2041 caggtgccaa tcttcaaaca tttaatggca gatgagctaa cagctttatc gctattagct
     2101 aaaccacata ccgttaaaaa gggaagtacg ttccattgta atgctcagtc gatatacatt
     2161 gttgagaaag gtgtttttgg tgtacaaaag tcatctgaat caatatattt atcccgaaca
     2221 tcatattttg gtcagttacc attctgtgaa aagagcacag gtgttgtcag ggcattgagt
     2281 gatgcatctg ttctggagtt tgccaatact gatttatatc aatttatctg taataatttt
     2341 aaagcactgc gcggttattg caggatactt gatcattata gaatcccgct cagtcaaact
     2401 gctacaacat tattaaaaaa taaagcctgt gtatacacta tttttagtac tgataaacat
     2461 tgtggcaaaa caacactggc aatatcagtt gccactgcct gctcatacta taaaagaaca
     2521 attttgctgg atgcctcata tgaagggata tctgtatttg acatgtgtgg taaaccactt
     2581 ataccgccgc tgtcgcaaaa aaatcccgat gatgtcaccg gttataaatt tattcagcag
     2641 cgtatagaaa aaatttcgga aaccatggac atcgccaata ttgcctatgg gtcaaaagta
     2701 aaaataaatc ctgatataat aaatccgcta ttattttatt tatcatttga atattcatac
     2761 atcgttattg attgttcaaa cagtgatatc catctgcgcg atgtgataat gaaaaattca
     2821 gacagtatta taaatgtcat taatgaaaaa tctgccatta atcatcaagt agtaaatgat
     2881 actcttttaa ctcagggaca acaggtttac aatgttgtta ataaacattt tgtaaaaata
     2941 caaaaaattg atcagccatc ctatgtgttc aaaactattc cagtagaaac aaatgaaacg
     3001 atttttcagg cagtattaaa aaataaaatg tataatgatg agctatttaa aaatatagta
     3061 agtgataaaa gggcgatggt attgtcccat gggatgtatg aaacgctata ctattccgga
     3121 gtattggaat cattgcggga agaaaaactt tttgatgtta tatatgctaa tggtataccc
     3181 tatgcagtat tgatgtgtta tttggcaaca caaacaacag aagattataa gaaattattg
     3241 tttgattatt tttctgatga ttcattacat acattctttg atattgcgtt tcctgataag
     3301 tatctgttta aaaataaacc attgcacaac tttgcttcag ctataagtca tataaaaaga
     3361 attgaatgga atgcaatact tccttataca cagttaatgc ataataatac gcaaaggttt
     3421 ttctctaccg gtgatgctga aaaaattatt tccgctaatt tagcttttag tcccttattt
     3481 gaagggatat ctattcatgg tgatgcctat ttttctgctt ttccgcttta ccccctgatt
     3541 cccgagtcat tactgagaac gcatataaat tatataggtt acattgctat tgctaatccc
     3601 tataatccaa tttctgtaaa aagtaagatt cctggattat atagaaaaat tgttgatttt
     3661 cttcctgatt gttatttata tgcctgtgat aatacctttt gttgcaatag attaatttct
     3721 ttaacgttac ctgatgtcac atcaacacat gatctgatta aacaatcgct taaaaatact
     3781 gatatgttta tttcaatgat aaagaaaatg ataaagtaaa tgataaagta aatgataaag
     3841 taaccaatta ggagggtgca tgagatcaaa agctaccaat atcttacaaa caggtgttct
     3901 tttaacaggc attatttata tcattattgg attattgtat ggtttcagtc ctatatactt
     3961 tgctaatatt tttggtatag aggtaaatcc cgactggtat aatttaataa aatacgatac
     4021 atttatagct ccgctttatc acttttccag gatatttgct ttattattag ctattgtagg
     4081 cataagtatg ttgctaccat tatttgatcc attaaaatat cgtgggatga tatattacca
     4141 tagcatacta tttcccttag tttctgtgcc atctcttttg ataaatggta ttgcctataa
     4201 acatttgcct cttacaatat gtggttgcct tttattgata atttttctct ttgtaggttt
     4261 tgggttaata attacaaaaa aatatgcaaa aatgggactg gaataactat aataaatgct
     4321 tgctttttat aattgaacat gtattgattc actttaataa taaaaagaat ttttatgcag
     4381 ctttacttat gttatttcta atatttttat caggaggata tgatgaatct gaaaactaag
     4441 aaggttggga atattgtagt agtttatcta cagggacgat tggatgtcca tctttcagcg
     4501 gatatagaaa aggaaatcaa taaattaatt aaagatgatc ccgcttctca tctactgctg
     4561 aatcttgcag gtgttgaata catgagcagt agtggtttaa gaatttttgt ttctactatg
     4621 cgtatattaa aagaatcaaa tcgcaaatta aagatatgtt caatgaataa cgctgttaag
     4681 aaaatttttg aggttgtcga actcatggat atgtttgaaa tctatgaaac agaagaagaa
     4741 gctattgctt cgtttaaata atgtttatgc tgtcgtcttc aggtaagtcc aaaagttcca
     4801 aacaacagtt atttaaaaca ttgctatcgc aaaacgctga gtatgagact atggcaatac
     4861 ctttggaatt atctgaaggt ataaaaaatt ttgaaattta tgtaaaacac aactattttt
     4921 catggccata ctggatagct cattatacta atagggaaaa tgttagaata cagtctctcc
     4981 cgtttatttt atataacaat cattgcagaa catggtatac aataaatagt tttttaaaca
     5041 gtggagcaat aaggatagat ccttgcggta tgattcatcc tgactgggca aaatggtcag
     5101 ttgaatgtat ggtctactct gatggggcat atattaaatt tcatgatgat acatgtatac
     5161 atcaacaaaa tgaaagggga gttataaaaa catcgtatca gcatcattta tttcgttttt
     5221 tacagtcagt gtatattgct catacaaatg ttgatgaagc gatagttatc aataaaataa
     5281 atattaaaaa aaagaaccaa tcctatttca tcatagcaat cagaccttat acaacaacaa
     5341 cgttatccgg catcaattca atcgagttgt taaaaaataa tttagtgaaa atcaatgatg
     5401 atcatgcatg ttcattaatt gaaaaaccgg attatattct tgttggtaat gaaatcatag
     5461 gtgatattga tatattacag aaagatgata aatataggat aagtagttca agtggtatgg
     5521 cgacgatggc ttttgcatat gaattgaaaa atacggatca tgaatatggt attcgcatcg
     5581 ctaccggtaa aaatacaaaa ttatccgttt caacattatc tgttaaatca gctcttaatg
     5641 attataccag ctatgtagat agcataatga aacaggggat tgccatatca attggcaggc
     5701 atactataaa cgagtggact gatttttgca aattatcaac attggtgcat gcctctacta
     5761 tggcatctat aaaaacaact caactgcaaa ggttgaagca atattactat atttcttcaa
     5821 ccttgaacag aatgggttat ctaaaacaaa ctgagcttgc cattgatact atcaaaaaaa
     5881 atataaagat tacggatgat tatgaaggtg tcctatcttc atcatttttt tgtttaatag
     5941 ttggcgattg ctataggaca ataaaagatt cctcttttgt gcaaaataat tattccttat
     6001 gcaagcaata tgcttattcc atcactgaat tttttaaaaa aataggtgat ataacaaaag
     6061 aaaataattt taaggataat cctcaaatag ccataaatgc gattatcaat attgcatata
     6121 ttatatctgc tcttgaaaat gtagcattta tgtcgcgttc gttggggctt tttggtgagg
     6181 aaacaaaaat atttagtgat ataaaaagac tgcagaatgt aatattaaag tcaatattta
     6241 ccataaacag tgaaacaaac ggtgaagagg agaaattaat tgagtggact cctaaatata
     6301 gtaaacctat acgaaaacaa aaaataatag taaaagacag ccttgagttt tcccattttg
     6361 taaatgtaaa tgataacagg attatatcat acagcccctt gctttaccct tatatgaagg
     6421 atataatgca accgtatata gtgaaaaata taattaatgc tatcatacat gcaggtaaag
     6481 gattgccttt atataacact ataatcagcg cggttgatat agtaaattct ttaagtcttg
     6541 ctaatgcaat gatctatgct catgataaaa gagctatcga tagtattaat gcaattatta
     6601 atttatgtaa taaacaaaaa tttttacctg attttcttga tcctgtaaca ggtcatgccg
     6661 ttgtaaatga ccctgtttca actattgctg tttcattatt atttcaagct atccgtaatt
     6721 tatgctttat agatcatcct gaacgactgg aaattttccc ggttccatgg gaagaatggt
     6781 ttaatgattg tcagatgaat ataaaaaatg ctcattcacg gtttggtata attagtttta
     6841 cggctacatc cacatcaaat gaatttgtaa ttcaatttac tgacataccc cggtttatac
     6901 cacatgatat tttagttaat cttccattta aagcaaaaat aaaaaaatca gatgatttta
     6961 ttgtcaaata tcagacagaa aaatcatggt gtattaatgg ctggccttca gagattagat
     7021 ttaaacgtta attactgttc tatcgataat aaagtagtaa ttgcggtggc agttgtaatt
     7081 ttaatactgg aaaatgaaaa atgatatcat atcttgtaat atcactatta attatcagtg
     7141 gatttgtata ttatttagta tatatcagac caaaatttaa tcccttaaat aaggcagaac
     7201 aatttttaaa aacaaatcaa ttgcatgatg caattattga atataaaaaa gtcatcgatg
     7261 aatatcctga taattttgta gtccatcatc gtctggctga actttattta acaatgggtg
     7321 aaattgatca ggcagtgatt cacttagaga aaatattaga aataggtaaa tttaactatg
     7381 aggttgatca actttctgta ttaaaaaaat tagcaaagtc atattatgtc cgtgatgagg
     7441 ttgaaaaagc attcaagatg tatatggata tattaaaaaa ttttcctgct gatattgatt
     7501 ctctttacca tgtatcattc attttgttaa gtcaggagga atttgatatt gcacaacgtt
     7561 attttgaaag attattaaaa cttaaaaatg atagttacga gattttattt ggagcaggaa
     7621 tctgtaatta tcaaaatatg cgaatccagg aagcgattga taattttaga ttggctctac
     7681 aattaaaacc acaatcagaa atagctcaaa ttgcattggc ttttgccttt aaccgtaaac
     7741 gtgattaccg acaggctctg acgtatatta caaaagtggc tgaatctgca caggatcctg
     7801 acgttcagta tttagcaaaa cgattccagg cttttttata tatgcgcctt aaacgtcatg
     7861 atgagggtat taaactattt caggatttac tggaaatagc ccgaaagaat gctaatgatg
     7921 atgctgaagc tttagcttta catgatcttg ggtatgcttt gttaaaaaat gaacaatcat
     7981 cattagcata cgatgtgtgg aatgaactat accgtaaaga tcgaaattac aaaaacatac
     8041 aatttcttgt gacgcaattg cgaaaagaaa tggaggctga taaatttgtt aaagaagaat
     8101 cagcctctat aacagaaact attacagcat ggatggagga agcttttcca caaaatttac
     8161 tatgggatat gtgtggatta aaaggcagta aaagatacaa tttaaaagat gttgttgtga
     8221 caacacgtat aacttttgat gatgggaagc tatccagtac ttctgatgag attatacaaa
     8281 agtatattgc tttagatact gaaaatttca ggattatatc aaatagattg gtgtcaaaag
     8341 tgggatttaa agtagatcag attttgcaaa cataccgtga agctgatggt gttgattttt
     8401 tagctgttaa tcctgaaaca aaagataagg tgcttgtatg ggtcaggaga tggacaaaga
     8461 caaatgttgg tgaaatcacc ttgaggaatt ttgctcaggc aattaatgat atgaaagtta
     8521 agcaggggat attaataacc acggctgatc tgactgatat tgcaaaggaa actttaaagc
     8581 aactatcaaa gatatcagta atctatcctg atgaaataga aaaattactg cgaggacttc
     8641 tttaaatgcc atttgataat ttcacatcaa caatgatacc tttgcagtca attaattata
     8701 tcaatgatta ttacaaaatt tcaaaaccat tgtctcctga attaataaaa tcaataaaac
     8761 aaaacggtgt gctggaacca gttatcctga ttgagggtaa cggttgttat attccggttt
     8821 gtggtcacaa caggatgctt gcatcaattc aaacagaatt ggccaatgta ccagcaaaaa
     8881 taatcaatgg gctttccgaa gatgatttca tggctataat aacattaaaa ctatacaaca
     8941 atttaatggg aactatcggc aaattaaaaa gtttatttat actaaaagag tattttaaaa
     9001 taaataaaaa acaattgatt gcgatagctc atgaattatc agtcccagat gctttgattg
     9061 aaacgacttc tttaaaaaaa attattggtc tgccatcaag tttaatgaat tatattgatt
     9121 caaaaaacat accttttaaa tttataatgc ctgtccttcg cttatcagat gaaagtattc
     9181 aacttgttgc tgcaatcata aatgcctgtc aactgcgtgt aaattatttc agggatattg
     9241 ttgtgatgat agaagatata tgtcgtaatc agcactatga aagcgttttg aaaaaaatta
     9301 ctgttctgat caataaggaa aacagaactg atgatgagat atatcgaaac atctatttgt
     9361 tacgatatcc acagtatagc gaatatgtag aaaaaattaa ttacttaaag aataaatttt
     9421 taagttatgg tttaaagata gaagtacccg aataccttga aggtaatgag gtgaacatta
     9481 ttattcaggt gaaaaagaat gaagttgtac acccgctgtt acagcaggac aaagaaatac
     9541 gtgcgttgat tcaatcaatt attgaattgc tttaaattaa tggatttatt taaaaaatgc
     9601 tgacatctct tgaagaatct tttgtttagt agtctcaaaa ttgtaaggag atttaaaacc
     9661 agcggcattt ttatgtccgc ctccaccgtt tgctattgca attgctgcaa catctaaatc
     9721 gcctttagtt cgcatgctta ctttaaccgg accgtcaaca tcctgtttta caacaacact
     9781 ggcaactaca ccattaatag ttaatggtaa attaatattt acttcgcctt ctgaaaaagg
     9841 tgcattcgtt gctttaatca tctctggagt taattccatt aatataagtt ttccaccaaa
     9901 atgtacttcc atggatgaaa gcatcaaagc acgcaatcta aatacttcta acgtgttatt
     9961 ttcatatatc cattgatata cttcgtatgg tttaattcca gatgaaatta attgtgctgc
    10021 aatagtaaat gtatgtgatg atgttttagg atatttaaaa cagcccgtgt caaaaacaat
    10081 accagcatag agtgcctgag ctgccttaaa tgatggtttt atgttatacc gttccattat
    10141 cgtgcagatt atttctgatg ttgatgatgc ctcaacttta atgtaatttg tttctaattt
    10201 attgttagca ccttcatggt gatcaataat aaaaacatct ttaacaatat tgtttaaata
    10261 atggtacgct tttccaatat tattaaaatc atttgtatcc aatacaaaaa tagtataatt
    10321 acttatattg tctatgatca ttttttcatc aaaaataatt atttcattat caggatcaat
    10381 aaaattaaac tttgtgggag ttggatctga attaataatg atagcatgtt ttcctaaatg
    10441 tagtagtatt tccttaaatc ctatttcagc gccaagacca tcaggatcag ggctctcatg
    10501 tgttgatatt ataaaatgat catgggcatt taaatatttt tcaaggttgg caaagtaatc
    10561 cattaagctt tctcgggtga tgcatatttt gcatatttac aaagcggaaa tcccattgat
    10621 tcacggatag caacatatcg ctcggcacag cgtcgggcaa tgttgcgaac tcttgtgatg
    10681 taacctacac gttcagtaac tgaaattgca cctcgagcat caagtagatt aaatacatga
    10741 gaacatttta aaacataatc ataagccgga agtattacct gaaccggttc ataatcaagg
    10801 atcttcaggc attctttttc atagagatca aatagttgaa ataataatgc ggtatcagca
    10861 atagaaaagt tataatgtga aaattcaaat tcactgcgat gatgaacatc tcggtaggta
    10921 agtgtatcat tccattgaat gtcaaaaacg ctctcaactc cctgaatata catgcagata
    10981 cgttcaagac cgtaggtgat ttctaccggt ataggatcaa gctcaatgct tccgcattgc
    11041 tgaaagtaag taaactgtgt aatttccatc ccatcaagcc aaacctccca tcctaatcct
    11101 gatgcaccaa gtgtaggtga ttcccagtcg tcttcaacaa atctgacatc atgttcttca
    11161 agtttgatgc ctaagttacg taatgaatcc agatataaat cctgtatatc aagaggtgat
    11221 ggtttcataa ttacctgata ttgataatag tgctgcagtc tatttggatt atcgccatat
    11281 cttccgtctg ttggacgtcg tgatggttca acatatgcta cattccatgg ctcaggacca
    11341 agtacacgta aaaaagttgc aggattaaat gtacctgctc caacttccat atcatatccc
    11401 tgtgcaatga tacaaccctg ttttgcccaa aagctattac aactggcaat gagatcctga
    11461 aaatacatgc gatagtctcc tgaaaataga attatgttac aatgtcattc ttagggatgc
    11521 agtaaagaag aatttcctta acaatgattc cataactaat ttgtgatctt attgttttgg
    11581 gaatattctt caaactttgc ttttattaac tggtgcaatt catcaaaatt agttttatta
    11641 ttaagcttaa aatagtcaag acagtcttca tctgcaatct caaatctaaa gttttttgac
    11701 tcaatgctgg taaagacata tgccagatag acaatgtaga tcaaatctct gtactgcgag
    11761 ggagccagat gcggtctgtg atgaaattca atagccttca ccagcgcatc attaaatttc
    11821 catttctggc agattaaact tcctaatgtg ctatggctta ctcctaagga tatctcttcc
    11881 agtattgaga tattatcaag ctgttttaag cctacaattt cccgtatttt ttgaatttca
    11941 tctggtttga tagaaagtaa taccataagc ccaatatccg aaagcaatga tgccagataa
    12001 gcaaattcag aaagccgttc cttgcgatat ttcatcgcga tccattgtgc ataaaatgct
    12061 gttttatatg aattgttcca gatggtttca aattttttat agcgggaatc aattatcttt
    12121 tgaacaccac tggcaacgag cagtgtattg ataccttttg tgccaatgat ttttactgct
    12181 tcctcaatag tttcaactcg ttttgatgtg atgtagcctg ctgaattggc aagcttcagt
    12241 atggcggtaa caagccccgg gtcgcgcttg atgctgtcgg ctatagcctt tattgtagat
    12301 tcaggatttg cacataagcg ctgtatttct agaatatttt gtggaaatga agggagtgat
    12361 tcaacctcat ttatgatttc ctgtgccatt ttaaccttta gatccatgtt gcggaagctt
    12421 tcatcaatgt ttatggtggc actggtgatc ttaccatttg tggacatgct aaatgcctca
    12481 ggaggaaaac cggcattctt catgagtatc aatgtaataa tcaaacctaa tcctgctcct
    12541 tccgaatcat ctaaaatgtc atcaaatgca tcggaaatac catggtaggt atatgctttc
    12601 tttattcgtg cattaatttt gttcatctct tcaggtataa tgggaacatt gtttattaca
    12661 tgaattttat gaatatcacc ggtaatggaa aagaataccc gtacaataaa gttggatttt
    12721 ccaagctctt caagaaactc gggatttcca acaaacacat cttctttaaa tgtttccatc
    12781 ccgttacggt attcttcttt tttattgata tccagatttt ttcgtttaaa gtaaagccgt
    12841 ttagcgttag ccttgatggc attattcacc agttctttta gtatgtttat aaacatatct
    12901 tttaaatata aaagatcaag ttgtgacaaa taaatactca gcaccctgat taaaagtgct
    12961 tcaacctcag gggttaatag tgaaaactgt aggtaaacgt cttcacctct tgttagtgct
    13021 tctttataat caactggctt acaaaaataa tgagattttt gctgtgttcc ttccaatatt
    13081 tacctcgtct acaaaatata acaagataat acatggtatt gtgaatattg caagaaaatt
    13141 actatgaaac gtcaagtttt tattcaatta tacttttata tagtgtatcg gtttttttat
    13201 atttttatta atggaaaatg gtggcgatag taactctcaa ttttgttcat tattgtaaat
    13261 agactnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgat tatactaata
    13381 ctatcagaaa ctgttgtgca agcgaggtac atatgtctgt tgtttatttt acagatttgc
    13441 atatgagcag taaggagaat atcgtcagca aaattaaaaa gcttttttac aaattatctg
    13501 caaatgagat aatcaatgaa aaagattttg ttgcaatcaa aacacatttt ggtgaatatg
    13561 gcaacattgc ctatacgccg cctgtaataa tacatacatt tgttgattgt gttaaggatt
    13621 tgaaagcaaa tccatttgtt actgattcca ccacattgta tcgaggaagc agaagtaacg
    13681 ctgttgatca tctgaaaaat gctgctatga acggattttc tttagtttca tgcggtgcac
    13741 cggtgattat tgccgatgga ttacgaggaa acgattttaa agaactccct gctaccggta
    13801 aatggtattc aactattaaa gtagcttcag caattcacga tgctgattcg atggtagttg
    13861 tgagccatgt aaaagggcat gaactgtatg gttttggtgg tgctataaaa aatattgcta
    13921 tgggatgtgc gccaccttcc atgaaacaat cattgcattc tgatttaaag cctaaagtaa
    13981 aacaaacact atgtacctca tgtggtgcgt gcattaaacg atgtccggca tttgccattt
    14041 catttgatgc aaataaaaaa gcatttatca atagtgataa gtgttttggt tgcggagaat
    14101 gtacagttat ttgtgtttat aatgctatac cggtattgtg gaaaaccgac cataccacac
    14161 tgtatgagcg aacggctgaa tatgttaatg cttttatgaa acataaggaa aataaagttt
    14221 tattttttaa ctttatcatg aatgtatctc ctgaatgtga ttgttgctac tggaatgata
    14281 tccctatagt tcccaatata ggaatagttg cctcaaccga tcccattgca gttgataaag
    14341 catcaataga tctgataaat aaagcaactc cgatgccaga ttcacgaata gctcataagg
    14401 atagctcaaa agataatatt gaagcgttat acccggttaa atggaactgt ctctttgaat
    14461 atgctacatc tattggagcc ggtaatatgg aatatgaatt agtgacaata taacattgat
    14521 atttttataa aatggagtga ggttttatga aaagtccaca tgattttcct gcaatcaatc
    14581 ctaagagacc tgatggtata aaaccaaacg ggataccata taaggttatg gtaattgatg
    14641 ataaagagtt ccatcgcaag cagattattc aaatactgga atctga
//
LOCUS       DAOSOX010000043        14639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4145_length_14639_cov_10.211327, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000043 DAOSOX010000000
VERSION     DAOSOX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4145_length_14639_cov_10.211327"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..593
                     /locus_tag="PLX22_04810"
     CDS             6..593
                     /locus_tag="PLX22_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT19250.1"
                     /translation="MIFCEFEPGNDFYFIQSGRVEIVKIINNREKTLDILGAGDVFGE
                     MAILEEEPRSASAIAIEHVKLLRFRRENFDALLQGNPQLAYKLLVIFSKRIYDAKRRL
                     MILLLDDPQLKIMDVMNMLAEQDPHLDVQEPVTLRVTIQEIANWAGMQINDVQKLLTH
                     LNKLGKIELFNDRVIVRNIRDFQRMVNSRRKSMYA"
     gene            594..667
                     /locus_tag="PLX22_04815"
     tRNA            594..667
                     /locus_tag="PLX22_04815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:628..630,aa:Arg,seq:cct)
     gene            730..1809
                     /gene="prfA"
                     /locus_tag="PLX22_04820"
     CDS             730..1809
                     /gene="prfA"
                     /locus_tag="PLX22_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT19251.1"
                     /translation="MESRLQQLLDRYHEIEQAMADPKTVNDQNLFKSLTKEHSKLFPI
                     VQKYNEYTKIKKETEEVTNALKEENEPDMVALLEDEKIQLESKMNEIEEQITQLLLPQ
                     DPHEGKDIIIEIRAGTGGDEASLFVADLYRMYIRYADLKGWKVEFIDANQTELGGFKE
                     IIFSISGDDAYSCLKFESGVHRVQRIPVTESGGRIHTSAVTVAVLPEAEENDIQINPD
                     DIRIDVYRSSGHGGQSVNTTDSAVRITHIPTGIVVTCQDEKSQHKNRAKALRVLRARL
                     FEKFERERMEKETALRRSQVGSGDRSERIRTYNFPQSRVTDHRINLTIYDLESVLDGE
                     LDKIIIPLREYDMEQMLKASHVYHA"
     gene            1793..2662
                     /gene="prmC"
                     /locus_tag="PLX22_04825"
     CDS             1793..2662
                     /gene="prmC"
                     /locus_tag="PLX22_04825"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT19252.1"
                     /translation="MYIMHNNATIFDILSQAAHRLASISATPALDAEILMAHTLNIER
                     HTLPLHYSTHISKIIYKKFENLLLRRLQSEPIAYIIGYKEFYSLPFHVNPHVLIPRPE
                     TELIVDYALYYAHHNATILDIGTGSGAIAIAIKYNLTDCSVWAIDISADALRIAKKNA
                     ATLLGMNKIKFLQSDLFTSLPHVHFDVIVSNPPYINPEIYTTLQKDIFFEPKEALVAP
                     DHGFGIIKAIINDAYNYLTKDGVLIVEISDDLAHQIVNYTQSLPYTISIAKDYSGMQR
                     VAILHKTIKNISN"
     gene            2793..3704
                     /locus_tag="PLX22_04830"
     CDS             2793..3704
                     /locus_tag="PLX22_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19253.1"
                     /translation="MINGKEELLHDIRVANFIEEYRFSCISKRDKWYSFSNIKWDPIS
                     SEIKIEAIINLIDSSYTINQIIKQDTVPRKSIGNQRFKYTILEPLKRSKIFFKESDIT
                     GTILIQNYFPYYDIIHHSSISPNNVENMFNFYSDRCKAKVSVELHKKNNTTHKYFDCV
                     GFREHSWGLYPSHHISCDSRVVVHFRDQTISFRYIEYNGMSYSYGCISKKSGNLALVL
                     VELEFLSIKNSSFESSEFTYKDAQDDIDLIVSKPIKPHHIEVSKKLKKSHLHIVSFSD
                     FTVIGTNKKGYGIEEHLISYERLKNVL"
     gene            3714..5033
                     /locus_tag="PLX22_04835"
     CDS             3714..5033
                     /locus_tag="PLX22_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19254.1"
                     /translation="MTITLEQIRKLSIFLIILFLLLLYNIYSKLFFDWYGIDIIVRSY
                     SFLFSFFCIFNYTQIDIKQYKTIYQKRYRSYANFLLFFETRLIPFLFIYFVSTLHSLI
                     DTINKSNWPYAGYFGILDGRFTNIVFYSLILFIVLRYRLKPSLAIPIFIGASIAFYFL
                     DKAVYDTIIAGGRLIVFKTIKVAIITAVLLFEYFQLHILSVAAISLITGILLITGVMS
                     TYIMLYYQTEEPKTKNEISIKLLRYGIHWKLDNLKESIITSLNYRDFSIYSTYLTFLG
                     KPLDFTYDEWVRLLFTDTIEMSDDIASHLLKKEINIPFDSIIDYAYTASIIHNQKLQS
                     AQHLAQLAARFADGKENIIIDLFEKGNMSFKIWAMNVMQHHNKMIYIPPLLPHCTDIN
                     ETMSSTAYSTITKITGKNFSSQLNLPYNNPKVLRAIKDFYFQKNTIQ"
     gene            complement(4996..5631)
                     /gene="nth"
                     /locus_tag="PLX22_04840"
     CDS             complement(4996..5631)
                     /gene="nth"
                     /locus_tag="PLX22_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT19255.1"
                     /translation="MIDRGTSKKIITRLKKEYPDVAPPLKYANVYQLCIAVVLSAQTT
                     DNQVNRVTPILFKKYGDFATLAAANVHEVEHIIKPTGFYHTKAKHIIALSQQVIEKFN
                     GNLPADREQLMSLPGIGRKSANVILATGFGIPAIPVDTHVARVAHRIGYCSSKKVYEI
                     ETMLMKSIDESEWITAHFLFIYHGRNTCMARKPQCSHCPITVWCSFENKNL"
     gene            complement(5624..6154)
                     /locus_tag="PLX22_04845"
     CDS             complement(5624..6154)
                     /locus_tag="PLX22_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19256.1"
                     /translation="MSSKTLTIFCAGLVLIHLQCTTTKNVKHQHHWRFNDPYINQTII
                     SGWEHINKRHYEWAMLDFTRLIQKNYIDYDILFGAGLACYYTNDTVKAIDYCTGAIEQ
                     NPQHFEAYFYRAQCYLALNNKKKALDDLHTIVAMKLNQPFICGYYINNDDLADDAALQ
                     ERKKEAQKIIVAIVHD"
     gene            complement(6144..6749)
                     /locus_tag="PLX22_04850"
     CDS             complement(6144..6749)
                     /locus_tag="PLX22_04850"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOT19257.1"
                     /translation="MDVVVATRNNGKMKEIVAFDIPSVIFTSLDAYPEIGEIEETGAT
                     FTENALIKARYVCTVSGLPALADDSGLVIDALNGEPGVYSARYGNCATDDDRCRLVLS
                     KMINVPWDKRTARFICVMALVFPDGKEYIEEGICHGFIATRPAGDNGFGYDPIFYLPY
                     FDKTMAQLPLSVKNTISHRAKALQKVKNLLQTMVRQKSHEQ"
     gene            6995..7669
                     /locus_tag="PLX22_04855"
     CDS             6995..7669
                     /locus_tag="PLX22_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19258.1"
                     /translation="MEEKKQINILRIGVANLTELEELVKAFRLMNQYSKRRRFVVSRE
                     DLKDIYGNKIVEKAHDINISVVKLLQRNFKPDTVFKIFSSDEGIAIVTYSESMAGKDF
                     SMQLIETIGNIGGGIYKPFIDVVPNFHELFKLFEKGLSPKLVVVGYIPVKNVAQEVSM
                     YNEIKKFDPYIRLLDITNDDLKPKSFLKGAVLFHFDNTLKVWHRLLTKIINEYTKPYF
                     MEEISK"
     gene            7691..>8502
                     /locus_tag="PLX22_04860"
     CDS             7691..>8502
                     /locus_tag="PLX22_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT19259.1"
                     /translation="MDERKLQLFAPVILILTIITSISITIMLYLQKTNIIMIVLSCIL
                     SATLITLLIIFRLIHKVFFTNNIRKILSVVDNFKKGIFVQPSNEIKGNDLLSKMYGEL
                     LIASRHLDNIIKSQKNEIDKFRELYNNMMFSMNAYIMALDFNNKIIFTNELFLNKFQL
                     SPSEVNGKNLSDIFYFLTGTVLQAIEEIKSTHESIILEKAHLISHNRISIIADIKLSF
                     MISNNEPQIIMVMDDVTSKTKRDYQISLITRLSETIQKDDALDHILRTILTAV"
     assembly_gap    8503..8602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8603..9607
                     /locus_tag="PLX22_04865"
     CDS             <8603..9607
                     /locus_tag="PLX22_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19260.1"
                     /translation="FDLNEDTIFTQTLQSGETIHITDALHDQRVNDEIKNFIDVNEFV
                     IVPLIALNKIIGILIADNKFNQMPITQDSVDLLTVFAYQAALSIETYNNLELLKKEME
                     KIRHRQEAIVESEKLAAIGRIASHIAHEIRNPLVTVGGYSRRILQTANAQQIDIQKIK
                     KAAQIIMTETERLEKILANVMDFTRPSPFLLRFNNINEIIDDTASLLSNLFQERRVEL
                     SLQLQEVPLIKSDFNQMKQVMLNLLQNALDATPPYGIVEVKTYSDDKKVYIEVSDTGS
                     GIVEEDIYRIFEPFYTTKTTGVGLGLAIVKKIISDHNGDIKVINKERGAVFTISIPLP
                     "
     gene            9700..10059
                     /locus_tag="PLX22_04870"
     CDS             9700..10059
                     /locus_tag="PLX22_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT19261.1"
                     /translation="MKKILIVEDEQHQRELYAMELQEEGYEVDQATNGKEAVDKVKAN
                     KYDCVILDIRMPEMDGIEALGKILSRDKKVPIIIYTAYSNYKSNFMTWTADAYITKSS
                     NLKELKDKIKELVSTRS"
     gene            10059..11279
                     /gene="glgC"
                     /locus_tag="PLX22_04875"
     CDS             10059..11279
                     /gene="glgC"
                     /locus_tag="PLX22_04875"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT19262.1"
                     /translation="MDLQNVLKSTLVFILAGGQGERLYPLTKDRSKPSVHFAGIYRII
                     DFSLSNCLNSNLTRIIVLTQYKSLSLDRHIKTAWNLYFRESGNFIDVVPPQQRFDENW
                     YMGTADAIFQNIYIIQHEKPTYVIILSGDHIYKMDYQKMLKYHIEKNADMTIAACEMP
                     INEAHRFGVLETDSNLKVVGFEEKPQYPKPVPGTENAAFVSMGIYIFTTDKLVRYVSD
                     DHKSDSSKDFGKDIIPKIYETENVFAYNYVANEGERAYWRDIGTIASYYEAHRDIISI
                     SPILNLYDKEWPIRTYMEQWPPAKFIHNEEGRRGIAINSLIANGSIISGGYVEQSIIS
                     PGVKVNSFSEVRGSIIMHGSTIGRYAKIQNAIIDKDNHIPEHMQIGFDLEEDKKHFMV
                     SPEGIVIIPKSYVQ"
     gene            11399..13456
                     /locus_tag="PLX22_04880"
     CDS             11399..13456
                     /locus_tag="PLX22_04880"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOT19263.1"
                     /translation="MSCNFIFCLHNHQPVGNFDHVFQWAYNDCYRKTLDILYKYPEFK
                     FAIHNSGPLLEWIEDHDASYCDTLALMVERGQVEVIGGGFYEPIFSIISDNDIHGQLS
                     LMQSFCKKRFGVEPAGFWTAERVWDPEIPSLVYDYKLKYTLLDDIHFRYAGIPESGLY
                     GYYITERLLKPLYVFPIDKFLRYSIPFKLPHETIDYFKRQCDKYGHCAFVYGDDGEKF
                     GVWPQTFQWVFEEKWLQNFIEAILKEDFISLIHPGEYIQYNPPIDRVYLTQGSYYEMS
                     EWALPADSGEQLVQLHNEIKNAGREAEFYPFLKGGVWNNFLNKYPESNAINKRTLYLS
                     REINAIEIKTGKDMSQAKTELYRAQCNCAYWHGLFGGIYLTHLRSALYKHIAVCEDMI
                     CAEKKLNTPEIIQGDFRNEGHEQIFLRNQTCSIIVNPAFGATISEFTNRKKRTNVFDT
                     IARRKEAYHSILHNIKDDDKNATNVQSIHEMVTVKEKGLQKYLVYDSSRRYSCKDLLF
                     TSLPQPDDLMFNRIDYIDCSLHPFSCTMNDTTIQCDSSSDVLPAISKTFSIHATKPIL
                     TVHYDIEGYNGWFGLEFTINMLVPHSKECYYAIEGVAAENAYLDSMGTSSGITQVTMV
                     DTTRDVSFSLQTSKPFILVRYPVYTVSQSDSGFEKNYQGSCMVLCFETNNSLHLDCEL
                     TVQ"
     gene            complement(13527..13895)
                     /locus_tag="PLX22_04885"
     CDS             complement(13527..13895)
                     /locus_tag="PLX22_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HOT19264.1"
                     /translation="MDDQEKLPERILVADEVASILTGDNIVKALANCKVDTPEKEHAI
                     EFVKAHYNFIQEIVQNDIQRKIIRSDFEIKDLVAHVNALMQSKDEYLFTMIVMHSPKH
                     YQQIQKSLLQEINKKEKEKS"
     gene            complement(14054..>14639)
                     /locus_tag="PLX22_04890"
     CDS             complement(14054..>14639)
                     /locus_tag="PLX22_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19265.1"
                     /translation="DVGAECIIVGDVTTTDQSNVLQQYSANMKSKQAILNLKAIDVYT
                     GSILASTSTNMPGAHINAEAASKKAIQSALVKILGSTDEETGKFKSGPFMNQITKKFL
                     LAATHRMITLNIVGLDYNDLTKFRDILQNRIRGIQKVYVRGQAGKQSKIDVEFAGKTT
                     DLADELVGKASSIGFKIEVKETYPNKIMLIAQKL"
BASE COUNT         4842 a         2550 c         2507 g         4640 t
ORIGIN      
        1 acgatataat tttttgcgaa tttgagccgg gcaatgattt ttatttcatc caatcaggaa
       61 gggttgaaat tgtcaaaatt attaataaca gagaaaagac tcttgatatt ctgggcgctg
      121 gtgatgtatt tggtgaaatg gccattttag aggaagagcc tcgctctgca tctgctatcg
      181 ccatagaaca tgtaaaatta ctaagattca gaagggaaaa ttttgatgcc ctgctacagg
      241 ggaatcccca attagcatat aagttgctgg ttatcttttc taaacgcatt tatgatgcaa
      301 aacgccggct catgatactg cttctggatg atcctcaact caagattatg gatgttatga
      361 atatgctggc agagcaggat cctcatcttg atgtgcagga accagtaaca ttacgcgtta
      421 ctattcagga gattgcaaac tgggcaggta tgcagataaa tgatgttcaa aagctattaa
      481 cacatttaaa taaactggga aaaattgagc tgttcaacga tcgtgttata gttcgcaata
      541 tcagagactt ccagcggatg gtaaattcta gacgcaaaag catgtatgcg taggctcccg
      601 tagctcaggt ggatagagca gtagtttcct aaactatgtg ccagaggttc gagtcctctc
      661 gggagcagag atgaaaataa catatacatt agcgggctta tcccgcttct ttttttaggg
      721 gtattcacta tggaatcacg gttacaacaa cttcttgaca gatatcatga aattgaacag
      781 gcaatggcag acccaaaaac cgtaaatgac cagaacctgt ttaaatcttt aacaaaagag
      841 cattcaaaat tatttccaat agtacagaaa tataatgaat atacaaaaat taaaaaagaa
      901 actgaagaag ttaccaatgc gttgaaagaa gaaaatgaac cggatatggt tgccctttta
      961 gaagatgaga agatacaatt agaatcaaaa atgaatgaaa tagaagaaca aataacacag
     1021 ttactccttc cacaggatcc acatgaaggg aaagatatta tcattgagat acgtgctggc
     1081 acgggaggtg atgaagcttc tctatttgtg gctgatttat acaggatgta tatccggtat
     1141 gcagatctta aaggctggaa ggtagagttt attgatgcca atcaaactga acttggtggt
     1201 tttaaagaaa taatattttc aattagtggt gatgatgcat atagctgttt aaaatttgag
     1261 tcaggtgttc accgagttca aagaatacct gttaccgaat caggaggaag aatccacaca
     1321 tctgcagtaa ccgtcgcagt acttcccgaa gcagaagaaa atgatatcca gataaatcct
     1381 gatgatatcc gcattgatgt ctaccgttca tcagggcatg ggggacaatc ggttaatact
     1441 accgactctg ctgtacgtat aacgcatatt ccaactggca ttgtagttac ctgtcaggat
     1501 gaaaaatctc aacataaaaa cagggcaaaa gctctaagag tactgcgtgc gcgattgttt
     1561 gaaaaatttg aacgtgaacg catggaaaaa gaaacagctt tacgccgttc gcaggttgga
     1621 agtggcgaca gaagtgagcg tatacgaact tacaattttc cccaatcaag ggttaccgat
     1681 catcgtataa acttaactat ttatgattta gaatcagtac ttgatggtga actggataaa
     1741 atcatcatcc ctctcagaga atatgatatg gaacagatgc taaaagcttc ccatgtatat
     1801 catgcataac aatgctacta tttttgatat cctgtcacaa gctgctcaca ggttagcttc
     1861 aatttctgca acaccagccc tggatgctga aatattgatg gctcatacac tcaatattga
     1921 acgtcatact ctcccattgc attattctac tcacatcagc aaaataatat ataaaaaatt
     1981 tgaaaattta ctattacgca gattacaaag cgaaccaatt gcttatatta taggttataa
     2041 agaattttat tcacttcctt ttcatgtaaa cccacatgtc ctgatacctc gtccagaaac
     2101 tgaactcatt gttgattatg ccctttatta tgcacatcac aatgcaacaa tacttgatat
     2161 tggaacaggt agtggcgcaa ttgctatcgc aattaaatat aacctaacag attgttctgt
     2221 ctgggcaata gatatatctg cagatgccct gcgcattgca aaaaaaaatg ctgccacttt
     2281 attagggatg aacaaaatta aatttcttca aagcgatttg tttacatcgt tacctcacgt
     2341 acactttgat gtgattgtta gtaatccacc atacatcaat cctgaaattt atactacctt
     2401 gcaaaaagat attttctttg aaccaaaaga agcattagtt gcacccgatc atggttttgg
     2461 catcatcaaa gcaataatca atgatgctta taactatcta acaaaagatg gagtattgat
     2521 tgtagagata agtgatgatt tagcccatca gatagtcaac tatactcagt cattgcccta
     2581 tacaatttcc attgcaaaag attattcggg tatgcaacgt gttgcaattt tgcacaaaac
     2641 tataaaaaat ataagcaatt aagaaataat gataaattta aaataatttt attcgatact
     2701 gtaatataac tgatgtatca gcccaataag catggtagtg gagtaagcag accgctaaat
     2761 gagcattcca tctgaaaggg gaatcattgt gcatgatcaa tggtaaagag gaattactgc
     2821 atgatatcag agttgctaac tttatagaag aatatcgttt tagttgcatc agcaaacgtg
     2881 ataagtggta tagcttttca aatattaaat gggatccaat ttcgagtgaa attaaaattg
     2941 aagctatcat caatttaatt gattcttcat acaccatcaa tcaaattatt aaacaagaca
     3001 ctgtcccaag gaaatctatt ggtaatcaac ggtttaaata tacaatactt gaaccattaa
     3061 aaagaagtaa aatattcttt aaagaaagtg atatcacagg caccatactt attcagaact
     3121 atttccctta ttacgatatc attcatcatt catcgatatc accgaataat gttgaaaata
     3181 tgttcaattt ttactctgac cggtgcaagg ctaaagtttc agttgaattg cacaaaaaaa
     3241 ataatactac tcataaatat tttgactgtg taggatttag agaacactca tgggggttat
     3301 atccttctca tcatattagt tgtgatagca gagtagttgt gcatttcaga gatcaaacaa
     3361 tttcgtttag atatatagaa tataatggca tgagctattc atatggttgt atttcaaaaa
     3421 aaagtggcaa tcttgcattg gtccttgttg agttagaatt tctttcaata aaaaattctt
     3481 cttttgagtc atcagaattt acctacaaag atgctcagga tgatatagat ctgatagttt
     3541 caaaacctat aaaaccacac catattgagg tatcaaaaaa actaaaaaaa tcccatctcc
     3601 atattgttag tttttccgat tttaccgtta taggaacaaa caaaaaagga tatggtattg
     3661 aagaacatct tatttcatat gaacgattaa aaaatgtgct ataatgtgaa tttatgacaa
     3721 tcacattaga acagatcaga aagctcagta tctttttaat tatcctattt ttgttattac
     3781 tctataacat atattcaaaa ctattcttcg actggtatgg tattgacatt attgtccgtt
     3841 catattcttt tttattttca tttttttgta tatttaacta cactcaaatt gatattaaac
     3901 agtacaaaac catataccaa aaacgatatc gttcttatgc aaacttttta ttattttttg
     3961 aaactcgcct tattccattt ttatttattt actttgtatc cactcttcat tctttaattg
     4021 acactataaa taaaagcaac tggccatatg caggatactt tggcatttta gatggacgct
     4081 ttaccaacat tgtattttat tcattaattc tttttattgt tttacgctat agattaaaac
     4141 cttcattagc catccctata tttattggag catctattgc cttttatttt ttagataaag
     4201 cagtatacga tactattata gccggaggga ggcttattgt tttcaaaaca ataaaggttg
     4261 caattattac agccgtctta ctgtttgaat attttcaatt acatatactt tcagtagcag
     4321 ctatatcatt aattaccgga atactattaa taacaggggt tatgagcaca tatattatgt
     4381 tatattatca aactgaagaa ccaaaaacaa aaaacgagat atcaataaaa ttgctacgct
     4441 atggaataca ttggaaatta gataatctga aggaatccat aataacatca ctgaactata
     4501 gagatttttc tatatattct acatacctta cctttttagg gaaaccactc gactttacct
     4561 acgatgaatg ggtaaggctt ctttttactg ataccattga aatgtcagat gatatcgcat
     4621 cacatttatt aaaaaaagaa ataaatatcc cttttgattc cataattgat tatgcttata
     4681 ctgcttccat aatccacaac cagaaattgc aatcggctca acatttagca cagcttgctg
     4741 cacgttttgc cgatgggaag gaaaacataa tcattgattt atttgaaaag ggaaatatgt
     4801 cgtttaaaat ctgggcaatg aatgttatgc agcatcataa caaaatgatc tatataccac
     4861 ctttattgcc tcattgtacc gatatcaatg agactatgtc atcaacagca tatagcacca
     4921 taacaaaaat caccggtaaa aatttttcat cacagctcaa tcttccctat aataatccaa
     4981 aagtattaag agcaatcaaa gatttttatt ttcaaaagaa caccatacag taatggggca
     5041 atggctgcat tgaggttttc ttgccatgca ggtattcctg ccatggtaaa taaatagaaa
     5101 atgggcagta atccattctg attcatcaat gcttttcatt aacatggttt caatttcata
     5161 aacttttttt gaactgcaat aaccaatcct gtgagcaacc ctggctacat gcgtatccac
     5221 cggtattgca ggtataccaa atcctgtagc taaaatcaca ttagctgatt tccttccaat
     5281 gcctggcaaa ctcatgagct gctccctatc tgctggcaga tttccattaa acttttcaat
     5341 aacctgttgc gacaatgcaa taatatgttt tgccttggta tgataaaatc ctgttggttt
     5401 tattatgtgt tcaacttcat ggacatttgc agcagccagg gtagcaaagt caccatactt
     5461 tttgaacaat attggtgtta ccctgttcac ctgattgtcg gttgtctgtg ctgataaaac
     5521 aacagcaata cagagctggt atacattggc atattttaat ggcggagcaa catcagggta
     5581 ttcttttttt aaccgtgtga ttattttttt tgaagtgcct ctatcaatca tgaactatcg
     5641 ccactattat tttttgtgcc tctttttttc tttcctgtaa cgctgcatca tccgctaaat
     5701 catcattgtt gatgtaatac ccacaaataa atggctggtt taatttcatt gccacaatgg
     5761 tatgcagatc atccagtgct ttttttttgt tgttcaaagc caggtaacat tgtgccctgt
     5821 aaaaatatgc ctcaaagtgc tgtgggtttt gttcgatagc tcctgtacaa taatctattg
     5881 ctttgacagt atcattcgta taatagcaag ccagtccagc accaaacagg atatcataat
     5941 caatatagtt tttttgtatt agacgggtaa aatccagcat agcccactca tagtgccgtt
     6001 tatttatgtg ttcccagcca cttattattg tttgattgat atatggatca ttaaaacgcc
     6061 agtgatgttg gtgtttaaca ttctttgttg tagtacattg taaatgtatc aataccaaac
     6121 cagcgcaaaa aattgttaag gttttactgc tcatgggatt tttgccttac cattgtctgt
     6181 aatagatttt ttaccttttg taatgccttt gccctatggc ttatagtatt ttttactgac
     6241 agaggaagct gtgccatggt cttatcaaaa taaggaagat aaaatatggg gtcataccca
     6301 aacccgttat caccagcagg ccgtgtggcg ataaaaccat ggcatatacc ctcttcgata
     6361 tattctttac catcagggaa taccagtgcc atcacacaga taaagcgggc tgtccgctta
     6421 tcccacggaa cattgatcat ctttgaaagt acaagcctgc aacgatcatc atccgttgca
     6481 caattgccat accgggcaga atatacgcct ggctcgccat tcaatgcatc aatcacaagc
     6541 cctgaatcat cagcaagtgc tggcagacca ctaacagtac aaacatagcg ggcttttatt
     6601 aatgcatttt cggtaaatgt tgctcctgtt tcttcaattt ctccaatttc aggatatgca
     6661 tcaagagaag taaaaataac cgaaggaata tcaaaagcaa caatttcttt cattttccca
     6721 ttgtttcttg tagcaacaac aacatccatg gtgccccttc acttaatggt tatatgataa
     6781 acatagaata ataaataaat gttgcatgta ttggtaaagc ctgaacaaag tgttattggc
     6841 catttgattg gagtactatt attaaaaata attgcacggt atgcatttaa taatcaatgc
     6901 aatactatta tatgtttcat gtatatattt tcaatattta ttgttgcatt ttcattgaaa
     6961 aaattgttac tatgatagta tattgttgat agctatggaa gaaaaaaagc agattaatat
     7021 attacgtatt ggtgttgcaa atcttactga actggaagag ttagttaaag ctttccgatt
     7081 aatgaatcag tattccaaac gaagaagatt tgtagtttca cgcgaggatt taaaagatat
     7141 atatggcaat aaaattgttg aaaaagctca tgatattaat atatccgttg ttaaattgct
     7201 acagcgaaat tttaaacctg ataccgtttt taaaatattc agctctgatg aaggaatagc
     7261 aatagttacc tattcggaat caatggcagg caaggatttt tctatgcaac ttatcgaaac
     7321 aatcggtaac atcggaggtg gtatatacaa accttttatt gatgtagtgc caaattttca
     7381 tgagcttttt aaattgtttg aaaagggtct ttcgccaaag ttagttgttg ttggttatat
     7441 tccggtaaaa aacgttgccc aggaagtctc aatgtataat gaaataaaaa aatttgaccc
     7501 ttatattaga ttgctcgaca ttaccaatga tgatttaaaa ccaaaatcct tcttaaaagg
     7561 agcagtctta tttcattttg acaataccct gaaggtgtgg caccgactat tgacaaagat
     7621 tatcaatgaa tatacaaaac catacttcat ggaagagatt tcaaagtaat gatacgctgt
     7681 gatatagtgc atggatgaaa gaaaactaca actttttgct cctgtcatat tgatccttac
     7741 tattataaca agcattagta ttacaataat gctttattta cagaaaacaa acattatcat
     7801 gattgtactt tcttgtattc tctctgctac tttgataaca ttattgatta tcttcaggct
     7861 gatccataaa gtttttttca ctaataacat tagaaagata ttatcagttg ttgataattt
     7921 taaaaaaggt atttttgtcc aaccttcaaa tgaaattaaa ggtaacgatt tactttcaaa
     7981 aatgtatggt gaattactca ttgcctctcg ccatcttgat aatataatca aatcgcaaaa
     8041 aaacgagatc gataaatttc gcgagctcta taacaatatg atgttttcga tgaacgctta
     8101 tatcatggca cttgacttta acaataaaat aatttttact aacgagttat ttttaaataa
     8161 atttcaacta tccccttctg aagtaaatgg aaaaaattta tcagatattt tttatttttt
     8221 aacaggtacg gtattgcagg caattgaaga aattaaatcg acacatgaat caataatttt
     8281 agaaaaagct catctcatat cacataaccg tattagtatc atcgctgata ttaaattatc
     8341 ctttatgatt tccaacaatg aaccgcaaat tataatggtt atggatgatg tcaccagcaa
     8401 aacaaagcgc gactaccaga tatcactgat tacacggttg tctgaaacaa ttcagaaaga
     8461 tgatgctctt gaccatattt tgcgtactat tttgactgcg gtnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nntttgattt aaacgaagat actattttta ctcaaacact
     8641 tcaatcaggt gaaacaattc atatcactga tgctttacac gatcaacgag tcaatgatga
     8701 aattaaaaat tttattgatg ttaacgagtt tgttatcgta cctcttatag cacttaataa
     8761 aattattggt atattaattg cagataataa atttaatcaa atgccaataa cccaggatag
     8821 tgttgattta ttaacagtat ttgcttatca ggctgcactt tcaattgaaa cctataacaa
     8881 cctggaatta ttaaaaaagg aaatggaaaa aatcagacat cggcaggaag caattgttga
     8941 atctgaaaaa ttagcagcaa tagggcgtat agcttctcat attgcgcatg agatcaggaa
     9001 tccactggta accgttgggg gatattcccg aagaatatta caaactgcta atgcacagca
     9061 gatagatata caaaaaataa aaaaagcagc tcaaattata atgaccgaaa ccgaacgtct
     9121 ggaaaagatc cttgctaatg ttatggactt tacccgacca tcaccttttt tgctgcgctt
     9181 taacaatata aacgaaatca ttgatgatac tgcatcatta ctatcaaatc tttttcagga
     9241 aaggcgtgta gaactatcgc tacaattgca agaggtaccg cttattaaat ctgattttaa
     9301 tcagatgaaa caggttatgc ttaatctgtt acagaatgct ctggatgcaa ctccaccgta
     9361 tgggatagtt gaggtaaaaa catattctga cgataaaaag gtttatatag aagtaagcga
     9421 tacggggtca ggaattgtag aagaagatat atacagaatt tttgagccat tttataccac
     9481 aaaaacaaca ggcgttggtt tagggcttgc aatagttaaa aaaattatat ccgaccataa
     9541 tggtgatatc aaagttataa ataaagaacg tggagctgtt tttaccattt cgataccatt
     9601 accgtaaata ttattttttt acttttaaaa aaaatgtctt tgattttttt tgtagtcact
     9661 atacattgat tattagaaaa gtataattgg gaggttgtta tgaaaaaaat tcttattgta
     9721 gaagatgaac agcatcagag ggaattatat gcaatggaac ttcaagaaga gggatatgaa
     9781 gtagatcaag ctaccaatgg caaggaagcc gttgataagg ttaaggcaaa taaatatgat
     9841 tgtgtgatac ttgatatacg aatgcccgag atggatggca tagaagcact gggtaaaatc
     9901 ttatcacgcg ataaaaaagt acctatcatc atctataccg cctattccaa ttataaaagt
     9961 aactttatga catggaccgc tgatgcatat attaccaagt catcaaattt aaaagaactg
    10021 aaagataaga taaaagaatt ggtatcaaca cgaagctaat ggatctgcag aacgtattaa
    10081 aaagtacact tgtttttata cttgcaggcg ggcagggtga acggctttat cccttgacaa
    10141 aagatagatc aaagccatca gtacattttg cggggatata taggattata gatttctcat
    10201 taagtaactg tcttaattca aatttaaccc gtattattgt tcttacccag tataaatcgc
    10261 tttctctgga caggcatata aagacagcat ggaatcttta tttccgagaa tcaggaaatt
    10321 tcattgatgt tgttccacca caacaacgat ttgatgaaaa ctggtatatg ggtactgctg
    10381 atgcaatctt tcaaaacata tatattatcc agcatgaaaa acctacatac gttatcatac
    10441 tatcaggtga tcatatttat aaaatggatt accaaaaaat gcttaaatac catattgaaa
    10501 aaaatgccga tatgacaatt gctgcctgcg aaatgccaat taatgaagca catcgttttg
    10561 gtgttcttga aaccgatagt aacttaaaag tagttggctt tgaagaaaaa cctcaatatc
    10621 caaaaccggt tccaggaaca gaaaatgctg catttgtatc aatgggcatt tatattttta
    10681 ccaccgataa attagtacga tatgtttctg atgatcataa atcagatagc tctaaagatt
    10741 ttggcaagga tattattcct aaaatctatg aaaccgaaaa tgtttttgca tacaattatg
    10801 ttgctaatga aggtgagcgt gcatactggc gtgatatagg aactattgct tcatattatg
    10861 aggctcatag agatatcata tccataagcc cgatactgaa cctttacgat aaggaatggc
    10921 ccataaggac ttatatggaa cagtggcctc cagctaaatt tattcacaat gaagaaggga
    10981 gacgcgggat tgccattaat tccctcattg caaatggctc tatcataagc ggaggctatg
    11041 ttgagcaatc aattatatca cctggtgtaa aggttaacag cttttctgaa gtacgtggaa
    11101 gtattattat gcatgggtca actataggcc ggtatgcaaa aattcaaaat gcaattatcg
    11161 ataaagataa tcatatccca gaacatatgc agataggatt tgatcttgaa gaagataaaa
    11221 aacattttat ggtttcacct gaagggattg tcatcatacc taaatcatat gttcaataaa
    11281 tttatagtaa atcccatccc attgcgatac attgatgcac aataccatgt atgagggatt
    11341 tactattgct aatacttctt cagaatgata cagtacatct ctattgacgg aggtaggtat
    11401 gagctgtaat tttatcttct gtctccataa ccatcagcca gttggcaatt ttgatcatgt
    11461 attccagtgg gcatacaatg actgttaccg gaagacactt gatattttgt acaaatatcc
    11521 tgagtttaaa tttgcaattc acaactccgg tcctctttta gaatggatag aagatcatga
    11581 tgcatcatat tgtgatacat tagcgttaat ggttgagcgt ggtcaggtgg aagtaatagg
    11641 cggtggattt tatgaaccta tattttcaat tattagtgac aatgatattc atggacaact
    11701 atcgcttatg caaagttttt gtaaaaagcg ttttggtgtt gaacctgctg gtttctggac
    11761 agccgagcgg gtttgggacc ccgaaatacc cagtctggtc tacgactata aacttaaata
    11821 tacactgctt gatgatatcc atttccgcta tgctggaatc ccggaaagcg gattatacgg
    11881 ctattatatc accgaacgtc tgctaaaacc actatacgtt tttcctattg ataaattttt
    11941 gcgatattcc atacctttta aacttcccca tgaaactatt gactatttca agcgtcagtg
    12001 tgataaatat gggcactgcg cttttgttta cggcgatgat ggtgaaaaat ttggtgtttg
    12061 gccacaaaca ttccaatggg tatttgaaga aaaatggctg caaaacttta tcgaagctat
    12121 tttaaaagag gattttatat cactcattca tcccggagaa tatatacagt acaatccgcc
    12181 cattgacagg gtttatctta ctcagggcag ctactatgaa atgtccgaat gggcgttgcc
    12241 tgcggatagt ggtgagcagc ttgtgcaatt gcacaatgag ataaaaaatg caggaagaga
    12301 agccgagttt tatccttttt tgaagggcgg tgtatggaat aattttttaa acaaataccc
    12361 cgagtccaat gcaatcaaca agcgcaccct ctatttatct cgtgaaatca atgctattga
    12421 aataaaaacc ggtaaagata tgtcacaggc aaaaacagaa ctgtaccggg cacagtgcaa
    12481 ctgtgcttac tggcatgggc tttttggagg tatctatctt acgcatttgc ggagtgcgct
    12541 ttacaaacat attgctgtat gtgaggatat gatctgtgcg gaaaaaaaac taaacacgcc
    12601 agaaataata caaggtgatt ttagaaatga ggggcacgaa cagatttttt tacgcaatca
    12661 aacatgtagc attattgtca atccagcttt tggtgctact atttccgaat ttaccaatcg
    12721 taaaaaacgc accaatgttt ttgatacgat agcgcgccgt aaagaagcat accatagcat
    12781 attacacaat attaaggatg atgacaaaaa tgccaccaat gtacaatcta ttcatgagat
    12841 ggttacagta aaagaaaaag gattgcagaa gtatcttgtg tacgatagtt ctcgaagata
    12901 ttcctgtaag gatttattat ttaccagctt gccccaacct gatgacctta tgtttaacag
    12961 aatagattac attgactgct cgttgcatcc attttcatgt accatgaatg atactactat
    13021 ccagtgcgat agctcatcgg atgtattacc agcaataagc aaaacttttt caattcatgc
    13081 aactaaacct attttaacag tacactatga catagaaggc tataatggat ggttcggatt
    13141 agagtttact ataaatatgc ttgtccccca ttcgaaagaa tgttactatg ctatagaagg
    13201 tgtagcagca gaaaatgcct atcttgacag tatgggtact tccagcggca taactcaggt
    13261 tactatggtt gatacaactc gtgatgtatc attttcattg caaacaagca aaccgtttat
    13321 acttgtgcgc taccctgttt acactgtttc acaatctgac tcaggatttg agaaaaatta
    13381 tcaggggagc tgcatggttc tttgctttga aacaaataac tcactccatt tagattgtga
    13441 acttactgta caatgaaatt gaatcgcaat gtgattatgg atatttttat ttcacacttc
    13501 aggaaaatca atgagtaaag tacttatcat gatttctctt tttctttttt atttatttcc
    13561 tgcaacaatg atttttggat ctgctggtag tgttttggag aatgcatcac tatcatggta
    13621 aacaaatatt catctttact ctgcatgagt gcattaacgt gtgccactaa atcctttatc
    13681 tcaaaatccg aacgtattat ttttctttgt atatcattct gcactatctc ctgaataaaa
    13741 ttataatggg cttttacaaa ttcaatggca tgttctttct caggagtatc tactttacaa
    13801 ttggcaagcg ctttaacaat attatcgccg gtcaatattg atgcaacctc atcggctact
    13861 aatattctct caggcagctt ttcttgatca tccattataa actcccgcta caaaaacttt
    13921 tcaaatttaa tgtatccaac catgcaacat gtgtcaatca aaataaatgc cagagaaact
    13981 attctctggc atttttgtgt aacaaagcta ttgttactat aagaaagagt aataatgtct
    14041 atgaaaaacc ttcttacaat ttctgggcta tcaacataat tttattggga tatgtttctt
    14101 taacctcaat tttaaaccca atgctactcg ctttaccaac caattcatca gctaaatccg
    14161 ttgttttgcc agcaaactca acatcaatct ttgactgttt tccagcctgc ccgcggacat
    14221 ataccttttg aatgcctcgt atcctattct gtaatatatc acggaattta gttaaatcgt
    14281 tataatcaag ccctacaata tttaatgtaa tcatacgatg tgttgcagct aacaaaaatt
    14341 ttttagtaat ctgattcata aatggacctg atttaaactt acctgtttct tcatcagtac
    14401 tgcctaaaat ctttacaagg gcactctgaa tagctttctt tgatgctgct tcagcattga
    14461 tatgagcacc tggcatgttg gtactggttg aagctaagat gctaccggta tagacatcga
    14521 tagctttcag gtttaagatt gcctgtttag atttcatatt tgctgaatac tgctgtaaaa
    14581 catttgactg gtctgttgtt gtaacatcac ctactatgat acattcagct ccaacatca
//
LOCUS       DAOSOX010000044        14630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4151_length_14630_cov_8.008302, whole genome shotgun sequence.
ACCESSION   DAOSOX010000044 DAOSOX010000000
VERSION     DAOSOX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14630
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4151_length_14630_cov_8.008302"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PLX22_04895"
     CDS             <1..467
                     /locus_tag="PLX22_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19266.1"
                     /translation="TSEEIAIIPVEVAHKYYNPVGMPVSFLKIFRNVTKVNISRPITM
                     SHFSASEALAEDTKMMLRLKLRHDTPLYPHYFVAHSLNKNSGIADIRKISDDVDSELK
                     LRNLKNSYDVSKIVQDGLDFLELNGYVMIKNTLVSVADNNTEAIRYFAGYLL"
     gene            complement(469..966)
                     /locus_tag="PLX22_04900"
     CDS             complement(469..966)
                     /locus_tag="PLX22_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOT19267.1"
                     /translation="MRKKQIVIIGSSDDTVHYDTAYVIGAFIASNSWILITGGRTGVM
                     EAASKGAHDNGGIVVGILPFDTFNDANSYCDIVIPTGIGFARNSINVLSADVVISICG
                     KSGTLSELSYAWQYGKPIISCVFTGGWSQRIAEEPIDDRQNGSTLYAAYSLDDVFSFL
                     YKLIQ"
     gene            complement(982..1938)
                     /locus_tag="PLX22_04905"
     CDS             complement(982..1938)
                     /locus_tag="PLX22_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19268.1"
                     /translation="MTGAQKAVSLSTMKALKRYTMKNDYTTFLKEIQQTILSFSEGDI
                     SFDEALQRYNAYTEKLKNDNIGKQYEDYIINCLGTHNAYNFSIFSALVAYLPGEPCCK
                     ICSFYGPDTFITTINSMCNKDFFSSPPRFLLDYSVHDTVMQNFSFHSSLIVNSESFSI
                     IFIAITSSSFFSKDSFLQFAAQLKTLMSPLTVTDSMSLDIPNCTLQYIHQTCSKLLPA
                     PLTLYGIHLISPFSTLSHFGLYSLLEVSSIIKNRLIDIFGQYAFALSMNRYLCISTNP
                     DINTRLFTIVYKEISIPAKFYKKTIEKEEEITPTLIEILHFR"
     gene            complement(1944..3101)
                     /locus_tag="PLX22_04910"
     CDS             complement(1944..3101)
                     /locus_tag="PLX22_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT19269.1"
                     /translation="MDFNLSEEQQMVKDMCRKFADNELAPKAEHYDKTHEFPWEHVKK
                     LGDMGMMGVVYPEEYNGSGMDYLAYAIAVEEISRGCASTGVIVSAHNSLCLSPIYYFG
                     TDEQKKKVLPKLCTGEWIGCFGITEPNAGSDAAGTKTTAEFKNGKWILNGTKNFITNG
                     GVASVAVVMAITEKGVGHKGLSMFIVEKGTPGFSVGKTEDKLGICASSTTELVFENCE
                     LPEGNLLGKKGDGFKIAMHTLDGGRVGIAAQALGIAQAALDAAAKYALERVQFNQPIA
                     RQEAIQWMLADMATEIQAARLLTYQAAMLIGSGADRRRFSKNAAMAKLFASETSHRVT
                     HKALQIFGGYGYIKDYPVERFYRDSRITEIYEGTSEIQRLVIASNVLNEYQ"
     gene            3278..4360
                     /locus_tag="PLX22_04915"
     CDS             3278..4360
                     /locus_tag="PLX22_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19270.1"
                     /translation="MVIKDNGMIKKSIALLAFCVMTSCGYFRVTDLKSTKVCTIPSGT
                     ALHAISINYNESGLQDMSFGVVLADDKVYVKDNIQRKVLVYDRNGNCKIAIGNVSGIA
                     DEKDVKKTQVSFDAIGTMVIDSENNLYVQNRLTSVQRISAAGTQIDMSPSFILVFNKE
                     GNLQYTIGQTGMPTVPFYYIEYLEIDNNDRLFVIARTYDIWSVYRFDKRQRDYYMTFT
                     KTHFVDMVDANTSYTGKIENIKILSSGDGFVLSVAYYHNTRFKYRKIYSYDIKSSKPT
                     EILKISEPKNELFTVIQNKYIYLWNVEDTNDVKFLLYNFDGTIVGKIRCKFPQQVLFS
                     DIGISNEGKLYSYHAYASGLEIYEWE"
     gene            4446..6044
                     /locus_tag="PLX22_04920"
     CDS             4446..6044
                     /locus_tag="PLX22_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT19271.1"
                     /translation="MKVVTALQMQKIDRLTINEYGIHGEVLMAIAGKSVADYIKTYLC
                     ADNIAVLCGVGNNGGDGYVAAYFLSQYIKTDIFVAGATQKLTPSSRIYFELCKKTAIP
                     VYELTDTILQTGIFNNYDIIVDALTGTGFSGTPKGLLLQGIEAINKSCKAVVSIDMPS
                     GLPADGILQSEAIVKAHVTVTMGLPKVNLVTYPGKDYAGHVVVADIGFPEVLLKSNDI
                     KRQLIDDDYIISNYRPQKNYDAHKGTNGHLLCIGGFDGMEGAIMLVSEAAFAAGIGLV
                     TIATTPKARTIIAGKIKEAITKEIELDDYADVFIDEMTHEVLKQKQFIELTERITTIL
                     QQLITSTRCSACAIGPGLGRSIFSAAVVKSAIAIVKERAIPAIIDGDGLFHCAMLHEN
                     FSPDKTCIITPHFKEASRLLNVPVDDIKQDRLSFAEKLAGATGAITVLKGPASIVSDG
                     VHTTINTTGNPALATGGSGDVLTGIIGSLLANRITPLVAASLGVYIHGKAADIYCDDS
                     GYTIMQAGDIIQNIRKAYGCLPWM"
     gene            6216..6650
                     /locus_tag="PLX22_04925"
     CDS             6216..6650
                     /locus_tag="PLX22_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOT19272.1"
                     /translation="MIHEKNFLYHLSQIRQKLFFYLEKQMAHRGIHDIAPSYGDILYV
                     LDKKGVMTLQQLGSLTMKDKSTVSSVVTRLERLGYIQKKISNDDKRLTLISITRKGKK
                     YKPVLLELSRAMNKKCFYGISKDEKAMLFKILQKIYENADRL"
     gene            6849..>7197
                     /locus_tag="PLX22_04930"
     CDS             6849..>7197
                     /locus_tag="PLX22_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19273.1"
                     /translation="MLIATMYDDVTQIKMSREVDGKGIYWVAAYLVDGLLVDTGCMFT
                     AQELLDFLKDKDILQVVNTHFHEDHVGANKLLQEQRGVAIYAHPDSIPLIASKQFLYP
                     YQEFVWGYPEPSVT"
     assembly_gap    7198..7297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7301..8215)
                     /locus_tag="PLX22_04935"
     CDS             complement(7301..8215)
                     /locus_tag="PLX22_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19274.1"
                     /translation="MYTVIVNNITRRFGDFTAVNAISFSVSKGEIFGLLGPNGAGKST
                     TIKMLCGLLQPDDGVGTVAGYDIVKQQWEIKNSIGYMSQKFSLYEDLSIEENLKFYGT
                     IYGLGTAQLKQRMHYIMEIVDIISHRHTVVKNLPPGIKQRLALGTALIHDPQILFLDE
                     PTSGVDPLMRRIFWDEIYKLANEGKTVFVTTHYMDEAEHCNRIALIIAGKIIALDTPQ
                     NLKENLPYKIIKITTNKFLQAYDILGDIHEIIEVALFGTDIHIVVDKNFASTATIKAL
                     LLSKGIESKLMPILPTLEDVFVMHARTV"
     gene            complement(8224..9369)
                     /locus_tag="PLX22_04940"
     CDS             complement(8224..9369)
                     /locus_tag="PLX22_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19275.1"
                     /translation="MKLSLNNIKIMIRKEFTLVIRDTNFRAIIIVPPILMTLVFGYAV
                     NTDVNDVLFSVYDRDRTAQSRIFIQQFVASPYFALHSYIDSPKQGMTLIEKGDTDFYL
                     EIENGFSKALKTGKPVALQVILDGTDSSRASVIQSYINSITLSYIQEQFISIIKMKIL
                     LQSQIGLQQSFIELIPRYWFNQDLKSVNFYLPGVIAMLIALITIMLTSMSIVKERETG
                     TMEQIKVSPLKPQEYMIGKLVPFAIIAFIDIIMVTAIAIFWFGIPFRGSFVFLLLSGI
                     IYIVSTLAIGLFISSVSSTQQQAMLSSLMFFFPSILLSGVIFPIYSMPKVIQLVTYLN
                     PMRYFAEIVRGIFLKGSGLYYLWHNIFILLVMSIVILRISIWQFGKR"
     gene            complement(9374..10513)
                     /locus_tag="PLX22_04945"
     CDS             complement(9374..10513)
                     /locus_tag="PLX22_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19276.1"
                     /translation="MISLLRIIAIAQKEWIHIYRDIRSLILSLFTPALLVLIFGYALN
                     MDVKNVTFVIYDQNRTSFSRQFAEIFMHSEYLQYSGYVHSFHDIDAIINSNKAVAALV
                     IPFDFTKKLKSGKSSQVQLLLDASDSMSATIASGYFNAIISQYNLQLQHNALNSLGYG
                     NSITPISVETRIWYNEELESKNFVVPGIIVIVLAIISALITSLTISREWERGTMESLI
                     TTPVRSYQIIIGKLLPYIFIGIFDVVITYLVGYFVFDIPIRGSFAELMLVSTLFLIGT
                     CTFGLVISSATRIQVLSIQVAMIATYLPTILLSGFIFPIKNMPIILQAITYIIPARYM
                     VSLIKGIAIKGLAASLLSTQIIFMVIFALVMVIVGIKKINLRYIG"
     gene            complement(10516..11427)
                     /locus_tag="PLX22_04950"
     CDS             complement(10516..11427)
                     /locus_tag="PLX22_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19277.1"
                     /translation="MIQVKGLIKQFNSIIAVNDVSLQLEKGELVGIVGPDGAGKSTLL
                     RIMAGILRPDTGTTLFNDNPYHNAIYNVKEHIGYMPQRFGLYEDLTVLENIMFFGKIF
                     GLREKEIKKTLPVLWNFSNLEAFKDRLAGKLSGGMKQKLGLACCLVHKPAILLLDEPT
                     NGVDPISRREFWKILYDLCVEGVTIVVSTAYLDEAERCNRVALMYHGSFLKVDTPTGI
                     KAMINKPLLEIVTNRPVEAEQILKTNPEYMHALRSGNAVRVFVDNVAMSLPAIEKLLF
                     SHKISVLTIHKTTPSLEDIFVEAIAPR"
     gene            complement(11427..12224)
                     /locus_tag="PLX22_04955"
     CDS             complement(11427..12224)
                     /locus_tag="PLX22_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT19278.1"
                     /translation="MKNKRIIPVIVVVVIVVGVTIYFEMLRYSGKNDNRIEGSGVIEV
                     TEVEVASKIAGRVVELPFDEGEEVKQGALIVKLAYDELSAQKLSALANLTNAEKNLKR
                     ARELFASGSISQRELDNAVAAYNVAKGNYDFVMATIDQAVLYAPVAGVVLTRNLEVGE
                     LALPGSSIMTLADLTKAWIKIYVNERYIGRVKHGQKAFVYTDSYPDKPFPGKVVAIAN
                     KPEFTPKTIQTKEERVKLVFAVKITIDNSNKELKPGMPADAVIVTGE"
     gene            complement(12214..13608)
                     /locus_tag="PLX22_04960"
     CDS             complement(12214..13608)
                     /locus_tag="PLX22_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT19279.1"
                     /translation="MKMICITRLLSLIIILFISIPVNALQKLTLNQAIDIALKNNFEL
                     LTAQQRLKQANEIIYQVWGMLYPALESEADAIRQYAENGFLSLSDGQYDLKFIQIKFG
                     INPGNFYNTLTQSYSNYKLAKEDVKKITSTIEYNVIKSYFDVILADEVITLRKNSINV
                     LQSNLLVVQNLFNTGTVPKFELLQAQVQLQSQEPLLIEAANNYNTALDLFNYHVGAKT
                     GTYTIAYEGFDSTIKKPQPVAIVNLLVEKALLYRPEVLQLSLRKDMVKDSIEATQSVY
                     LWPTFSIAGYYGKTYLLPNTSDVPLPFPGQSLDLSTITGNKEWQTTWQIRFAATYRWG
                     SLVPVDTVQGVEREEKEKLKEIEERIKQVKQSIIIAVRTDYGKLITAYETIIAQNKNI
                     ETASEGLRIAKESYRAGVIKNSDLLNAELQLTSARMGYIKAINDFYCTIAQLKKDTGF
                     DCTEIIFTEEMYEK"
     gene            complement(13611..14216)
                     /locus_tag="PLX22_04965"
     CDS             complement(13611..14216)
                     /locus_tag="PLX22_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19280.1"
                     /translation="MKTSKDKIIHAAIIEFANYGLDGARVDRIAKKAKINKAMIYYHF
                     KNKEALYADIVKNLAQTIFDTVQGSLQKAIEAGNPIIVIEDYARHMSSLDKHYFLIIM
                     RELSSGGKFIRKIVAPTIIEPITELVGKFYQLGNNKNMLKDIHPQYTMISIAGAVIFY
                     NLMRQILEGTSVYQKYFQESNVEQYIANLLQLLAHGIVKKE"
BASE COUNT         4550 a         2618 c         2749 g         4613 t
ORIGIN      
        1 aaacatcaga ggagattgct attatccctg ttgaagttgc ccataaatat tataaccctg
       61 ttggtatgcc ggtttctttt cttaaaattt tccgtaatgt aacaaaggta aatatttcac
      121 gacccataac catgtctcat ttttcagcca gcgaagcgct tgctgaagat actaagatga
      181 tgttaaggct aaagttgcgc catgatacac cgctgtatcc ccattatttt gtagcacatt
      241 cattaaacaa gaatagtgga attgctgata taaggaaaat atctgatgat gttgatagcg
      301 agttaaaatt aagaaattta aaaaactctt atgacgtttc aaaaatagtc caggatggat
      361 tagactttct tgagttgaac ggctatgtaa tgataaaaaa caccctggta tcagtagcgg
      421 ataataatac tgaagcaata cgttattttg caggttattt gctatagctt actgtatcag
      481 tttatataag aaagaaaata catcatcaag tgagtatgct gcatagagag tacttccgtt
      541 ttgtctgtca tcgattggct cttctgcaat cctttggctc catcctccgg tgaaaacaca
      601 gcttattatt ggtttcccat attgccatgc atacgatagt tcgctcagtg ttcccgattt
      661 tccacaaatt gaaataacaa catctgctga taaaacattg atactgttac gtgcaaaacc
      721 tattccagtt ggaatgacga tatcacaata actattggca tcattgaatg tatcaaatgg
      781 caaaatacca actacaatac caccgttatc atgcgcaccc tttgatgctg cttccataac
      841 gccagttcgg ccaccggtaa taagtatcca gctattgctt gctataaacg ccccaatgac
      901 gtatgccgta tcgtaatgta cagtatcatc agatgatcct atgataacaa tttgtttttt
      961 tctcatgaaa aagctcttta tttatcgaaa atgaaggatt tctatcaatg ttggggtaat
     1021 ttcttcttcc ttttcaatgg tttttttata aaattttgcc gggatggata tttctttata
     1081 aacaatggta aataaacggg tgttgatatc gggatttgta ctgatacaca gatatcggtt
     1141 catagaaaga gcaaacgcat attgaccaaa aatatcaatt aatctgtttt ttattataga
     1201 tgaaacctct aaaagtgaat ataaaccaaa atggctcaga gtgctgaagg gtgatatcaa
     1261 atggatacca tacaatgtaa gtggagcagg cagtaatttg ctgcatgttt gatgaatgta
     1321 ctgcaatgta cagttgggta tatctaagga catactatcg gtaactgtca atggactcat
     1381 caacgtttta agctgagctg caaattgaag aaatgaatct tttgaaaaaa aggatgatga
     1441 tgttattgct ataaaaataa tactaaaact ttcagagttc actatcaatg aactatggaa
     1501 agaaaaattt tgcattactg tatcgtgtac gctgtaatca agtaaaaatc ggggtggtga
     1561 tgaaaaaaaa tctttattgc acatgctgtt aatggtagtg ataaaggtat cgggaccgta
     1621 aaaagaacaa attttacagc atggttcacc gggtaaatat gccactaaag cggaaaaaat
     1681 agaaaagtta taggcgttat gggtgccaag acaattgatg atataatctt catattgttt
     1741 tccaatgtta tcatttttca gcttttcggt atatgcatta taacgctgca atgcttcatc
     1801 aaatgatata tcaccttcag aaaacgaaag gatggtctgt tgaatttctt ttaaaaaagt
     1861 ggtataatca tttttcattg tataccgttt taaagctttc attgttgaca gggacacagc
     1921 tttctgtgcc cctgtcattg atattattga tattcattaa gaacatttga tgcaataaca
     1981 agccgttgaa tttctgatgt cccttcatat atttcggtaa ttcgtgaatc gcggtaaaat
     2041 cgttcaaccg gatagtcctt tatataaccg taaccgccaa aaatttgcaa agctttatgt
     2101 gttacacgat gcgaggtttc agatgcaaaa agtttagcca tcgctgcatt ttttgaaaat
     2161 ctacgtctgt ctgctccact tccaatgagc attgcagcct gataggttaa aaggcgagca
     2221 gcctgaattt cagtagccat gtcagctaac atccattgaa tagcctcctg ccgggcaata
     2281 ggctgattaa attgtactcg ctccagagca tatttagccg cagcatccag agcagcctgg
     2341 gcaataccta aagcctgagc agcaatgcca acgcgtccac catctaacgt atgcattgca
     2401 atcttaaagc catccccctt ttttcctaaa aggtttcctt caggcagttc gcaattttca
     2461 aaaacaagtt cagtagttga agaagcacaa atgccaagct tatcttcagt tttgcccact
     2521 gaaaagcccg gagtaccttt ttcaactata aacatactca atcccttatg gccaacaccc
     2581 ttttcagtaa ttgccattac tacagcaacg ctggcaacac caccattggt aataaaattt
     2641 tttgtgccgt ttaaaatcca tttaccattc ttaaattcag cagtagtttt tgttccagcc
     2701 gcatcagaac ctgcatttgg ttcggttatt ccaaagcagc caatccactc gcctgtgcat
     2761 aattttggta aaaccttttt tttctgttcg tcggtgccaa aataataaat tggcgaaagg
     2821 cacaatgaat tatgagcaga aacgataaca ccggtggacg cacagcctcg tgaaatttcc
     2881 tcaacagcta tcgcataggc aaggtaatcc attccggaac cattgtactc ttccggatat
     2941 acaacgccca tcatccccat atcacctaac ttttttacat gttcccatgg aaattcatgt
     3001 gttttatcat agtgctcagc ttttggggca agttcattat cagcaaattt tctgcacata
     3061 tcttttacca tttgctgttc ttcagataga ttaaaatcca taaaccgatc tccttattat
     3121 atctgtataa taatgtcttt ttattattgt gtaaaataaa ctatgttttt gtcaaggcaa
     3181 taacgaacag tgttgtaaaa tgggatgctt ttttatgttg aaaaaaagat tgctacttta
     3241 tataaatggt tataaaaaat tatagtgggc gatagttatg gtaattaagg acaatggtat
     3301 gataaaaaaa tcaatagcat tattagcttt ttgcgttatg acctcatgtg gctattttcg
     3361 tgttaccgat cttaaatcaa caaaagtatg caccattcct tccggtacag cattgcatgc
     3421 aataagcatc aattataatg aatcaggact tcaggatatg agttttggtg tggtactggc
     3481 tgatgataaa gtatacgtaa aggataatat tcagaggaag gttcttgttt atgaccggaa
     3541 tgggaattgt aaaattgcaa taggtaatgt ttccggtata gcagatgaaa aggatgtaaa
     3601 gaagacacag gtttcatttg atgccattgg aacaatggtt attgattcgg aaaataatct
     3661 gtatgtacaa aaccgattga cctcggtgca gcgtataagc gcagcgggaa ctcagataga
     3721 catgtcgcca tcatttatcc ttgtatttaa taaagaaggt aatttgcaat ataccattgg
     3781 tcaaacgggg atgccaacgg tgccatttta ttatattgaa tatcttgaga tagataacaa
     3841 tgacaggctt tttgttatag ccaggaccta tgatatatgg agcgtttacc ggtttgataa
     3901 aaggcagcgc gattactaca tgacatttac aaaaacacat tttgtggata tggtcgatgc
     3961 aaatacctcg tataccggaa aaattgaaaa tataaaaata ctcagtagtg gggatggttt
     4021 tgttctttcg gttgcctatt atcataatac acggtttaaa tacagaaaaa tatatagcta
     4081 tgatataaaa agctcaaagc caactgaaat tttaaagatt tcagaaccca aaaatgaact
     4141 ttttacggta attcaaaata agtatatcta tctatggaat gtagaagata ccaatgatgt
     4201 taaattttta ttgtacaatt ttgacggaac aatcgttggc aaaatcaggt gtaaatttcc
     4261 ccagcaggtg ttattttcgg atattggtat aagcaatgaa ggcaaactgt attcttatca
     4321 tgcctatgca agtggtctgg aaatatatga atgggaataa aaaactattt aaacgcagca
     4381 caatgatgga atagacaatg ctaataaata tgattatcat gtatatatac gggaagtgat
     4441 tgaacatgaa agtagtaacc gctttgcaga tgcagaagat tgatagatta accatcaatg
     4501 aatatggaat acatggtgaa gtgttgatgg ctattgccgg gaaatcagtt gctgattata
     4561 ttaaaacgta tttgtgtgct gataatattg ctgtactatg cggggttggc aataacggtg
     4621 gtgatgggta tgttgcagca tattttttat cgcaatatat aaaaacggat atctttgttg
     4681 ctggagctac tcaaaagtta acaccatcat cgcgtatata ttttgaactt tgtaaaaaaa
     4741 ccgcaatacc cgtttatgag cttactgata caatactcca aacgggtatt tttaataatt
     4801 atgatattat tgttgatgca ttgaccggaa cgggtttttc gggtactccc aaaggtttgc
     4861 tgctacaggg tattgaagct ataaataaaa gttgcaaagc agtggtgtcc atagatatgc
     4921 caagtggact gccagcggat gggatactgc aatccgaagc tattgtcaaa gcgcatgtta
     4981 cggtaacgat gggattgcct aaagtaaatc tggttactta tccgggaaaa gattatgcag
     5041 gtcatgttgt tgttgctgac attggatttc cagaagtttt gctcaagtct aatgacatca
     5101 aacgacagct cattgatgat gattatatta tcagtaacta tcgcccacaa aaaaattacg
     5161 atgctcataa aggtacaaac ggacatttgc tctgcattgg tggatttgat ggtatggagg
     5221 gtgcgataat gcttgtgtct gaagctgcat ttgcagcagg catagggctt gtgaccatag
     5281 caaccacgcc taaagctcgc actatcattg caggtaaaat aaaagaggcg ataacaaaag
     5341 agatagagct tgatgattat gctgatgttt ttattgatga aatgactcat gaagtactga
     5401 agcaaaaaca atttattgag ttaacggagc gtataactac aatactacaa cagttgataa
     5461 cttcaacgcg atgcagtgca tgtgccattg ggccaggttt aggtagaagt attttttcag
     5521 cagccgttgt taagtcagca attgccatag tgaaagaacg cgctatccct gccatcattg
     5581 acggcgacgg cttgtttcac tgcgccatgt tacatgagaa tttttccccg gacaaaacat
     5641 gtattattac accacatttt aaagaggcat ctagattgtt gaatgtacct gtagacgata
     5701 taaaacagga tcgtttatcc tttgctgaaa aattggcagg tgcaacaggc gcaataacag
     5761 tattaaaggg tccagcttca attgtttctg atggagtgca taccaccatt aataccaccg
     5821 ggaatcctgc gcttgcaacg ggtggttccg gagatgttct tacagggatt attggttcgt
     5881 tgcttgctaa caggattacc cctcttgttg ctgcatcgtt aggtgtatat atacatggca
     5941 aagctgctga tatatattgt gatgattcag gttataccat aatgcaggct ggcgatataa
     6001 tccaaaatat aaggaaagca tatgggtgtt taccatggat gtgagattgt gtaataacat
     6061 ggtattatgc tgatagcttt tttcccgata agtaaaaaaa tacatacact gcccaaaagc
     6121 aagtcacaaa aatattggca ataaattgat aattgatgtt gacaaaataa taatagtttt
     6181 gtacaaaact aattatcgat gttgagaata tcataatgat acatgaaaag aattttttat
     6241 atcatttatc gcaaatacgt caaaagctgt ttttttattt agaaaaacag atggctcacc
     6301 gcggtattca tgatatagct ccgtcatatg gtgatatttt gtatgttctt gataaaaaag
     6361 gggtgatgac attacagcag ttaggttcat taaccatgaa ggataaatca acggtatcat
     6421 cggttgtaac caggcttgag aggttaggat acatacagaa aaaaataagt aacgatgata
     6481 aacgtttaac cttaataagt ataacacgta aagggaaaaa gtataaacca gtgttattag
     6541 aactatcccg tgcaatgaac aaaaaatgtt tttatggtat cagtaaagat gaaaaagcaa
     6601 tgctttttaa aatattacag aagatatatg aaaatgcaga caggttataa ttacaacttt
     6661 ttgtattaat aaattgttgg atcagtgata atgtttccaa tgtattgttt tgatttcatg
     6721 atggccggtt tatggctgct cataggggat gaaaagtata caaaccggaa caaatgctcc
     6781 cctcgccttg cgaaggagag gggccggggg tgaggttaat atttctatgt tggtaagagt
     6841 ggtaatacat gttgatagca acaatgtatg atgatgtaac ccagattaag atgtcacgcg
     6901 aagtggatgg gaagggtatt tattgggttg cagcgtattt agttgatggg ctgctcgttg
     6961 atacgggatg tatgtttaca gcacaggagt tgcttgattt tttgaaggat aaagatatat
     7021 tgcaggtggt gaacacgcac ttccatgagg atcatgttgg tgcaaataaa ttattgcaag
     7081 aacaacgtgg tgttgctatt tatgctcatc ctgattctat tccactgatt gcatcaaagc
     7141 aatttttgta tccataccag gaatttgttt ggggctatcc tgagccatct gtcacggnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta ttacactgtt cgtgcatgca
     7321 ttacaaagac gtcttctaat gttgggagta ttggcattaa ttttgattca atcccttttg
     7381 acaaaagaag tgctttgatt gtagcagttg atgcaaaatt tttatctact acgatatgaa
     7441 tatcggttcc aaaaagagca acctcaatta tttcatgtat atcccctaag atatcatacg
     7501 cctgtaaaaa tttattggtg gttattttta ttattttgta tggcagattt tcttttaaat
     7561 tttgtggtgt atcaagtgca ataatcttac ctgcaataat cagagctatt ctgttacaat
     7621 gttctgcttc atccatgtag tgggttgtta caaatactgt tttcccctcg ttagcaagct
     7681 tataaatttc atcccagaat atcctgcgca taagaggatc aactcctgat gttggttcat
     7741 ccagaaataa aatttgtgga tcatgaatta aagctgtacc taatgcaagt cgctgtttta
     7801 ttccaggcgg aagatttttt accaccgtgt gtctgtgaga tataatatca actatctcca
     7861 tgatatagtg catgcgttgt tttaattgtg cagtgcccag tccataaatg gttccataaa
     7921 attttaaatt ttcttctatg cttaaatctt cgtacagtga aaatttttgc gacatgtatc
     7981 caatactgtt tttaatttcc cattgttgtt taacaatatc atagccggca acagtaccaa
     8041 cgccatcatc gggttgtaat aatccgcaca acatctttat ggtagtgctt ttaccggcac
     8101 cattaggtcc aagcagtcca aatatttcac cctttgaaac agaaaaactt attgcattca
     8161 ctgctgtaaa atcgccaaaa cgcctggtaa tgttgtttac gattactgta tacataaata
     8221 tggttacctt ttgccaaatt gccatatgct gatacgtaaa ataactatgc tcatcaccag
     8281 aagaatgaag atattgtgcc acagataata taaaccgctc cctttaagaa atattcccct
     8341 gacgatttct gcaaaatagc gcattggatt taaataagta acaagctgga tgactttggg
     8401 catagaataa atagggaata tcacacctga caataaaatt gacggaaaaa agaacatgag
     8461 tgatgataac attgcctgct gttgtgttga tgaaaccgat gatatgaaca gcccaatagc
     8521 taacgtagag acaatgtaaa taataccaga taataataaa aacacaaagc ttcctctgaa
     8581 tgggatgcca aaccagaaaa ttgcaatggc agtaaccatt ataatatcaa taaaggcaat
     8641 aatggcaaag ggaacaagct tgccaatcat atattcttga ggtttaagcg gtgatacttt
     8701 aatctgctcc attgttccag tttccctttc ttttactatg gacatggagg taagcataat
     8761 agtgataagt gctatgagca ttgctataac gcccgggagg taaaagttta cactcttcaa
     8821 atcctgatta aaccagtagc gtgggataag ttctataaag ctttgttgca aaccgatttg
     8881 tgattgcaat aatatcttca ttttaataat acttataaat tgttcctgta tatatgaaag
     8941 tgttatggaa ttgatatatg attgtataac tgatgcacgg ctggaatcgg ttccatcaag
     9001 gataacctgc aatgcaacag gcttcccggt ttttaaagct tttgaaaatc cgttttctat
     9061 ttcaagataa aagtctgtat cacctttttc aataagggtc attccctgtt taggcgagtc
     9121 aatataagaa tgcagggcaa aatatggcga tgcaacaaat tgctgaataa aaattctgct
     9181 ctgagctgtc ctatcccgat catatactga aaataaaaca tcattgacat ccgtattgac
     9241 cgcatagcca aataccaatg tcatcaggat gggtgggaca atgatgattg ctctaaaatt
     9301 agtatctcgt attacaagtg taaattcttt ccttatcata atttttatgt tattcagtga
     9361 tagtttcatg taactatcct atatatcgta agtttatttt tttaatgcca actatcacca
     9421 taactaatgc aaagatgacc atgaagatga tctgtgttga aagaagtgat gcagccagcc
     9481 cttttatagc tatacctttg ataagagaaa ccatgtaacg ggcaggaata atataggtta
     9541 ttgcctgtaa aatgatgggc atatttttta tagggaaaat aaatcctgat aaaagtatcg
     9601 taggcagata ggtggcaatc atagctacct gtattgaaag tacctgaatt cgtgttgctg
     9661 atgagataac aagtccaaag gtgcaggtgc caataagaaa gagagtagaa accaacatga
     9721 gctcggcaaa acttcctctg atgggtatat cgaagacaaa atatccaaca aggtaggtaa
     9781 taacgacatc aaagatgcca atgaaaatat aagggagtag tttgccaata ataatctgat
     9841 atgagcgcac cggggttgtt ataaggcttt ccatagttcc tcgctcccac tcgcgtgaaa
     9901 tggtgagtga tgttataaga gcactgatga tggcaagaac aatcacaata atgccaggga
     9961 caacaaaatt tttgctttcc aattcttcgt tataccatat gcgtgtttca actgaaattg
    10021 gtgttatact attgccatat cccaggctgt ttaatgcatt gtgttgtaat tgcagattat
    10081 attgcgatat aattgcatta aagtatcctg atgctatggt tgcagacatg gaatctgaag
    10141 catctaataa caactgaacc tgtgatgatt tgccgctttt aagtttttta gtaaaatcaa
    10201 aagggataac gagcgctgct acggctttgt tgctgttgat aatggcgtca atatcgtgaa
    10261 agctatggac atacccacta tactgcagat attcactgtg cataaatatt tctgcaaact
    10321 ggcgtgagaa cgatgttctg ttctggtcat agataacaaa tgtaacattt tttacatcca
    10381 tattcaaagc atagccaaat ataagcacta ataatgccgg agtaaaaagg gatagtataa
    10441 ggcttcgaat atcacggtat atgtggatcc attctttctg agctatcgca ataatgcgca
    10501 aaagacttat catgttcacc tcggtgcaat tgcttcaaca aagatgtctt ctaatgacgg
    10561 cgttgtctta tgaattgtga gcactgatat tttatgtgaa aataaaagtt tttcaatagc
    10621 aggcaaagac attgcaacat tgtcaacaaa cacacgcact gcatttccgc tgcggagtgc
    10681 atgcatatat tcaggatttg tctttagtat ttgttcagcc tcaacaggtc tattggttac
    10741 aatttcaagt aatggtttgt tgatcattgc tttaatacct gttggagtat ccacttttaa
    10801 aaatgaacca tggtacatca gggcaacacg gttgcaacgt tcggcttcgt ctaagtatgc
    10861 ggtgctgact acaatagtta ctccttccac acacagatca tatagtatct tccagaactc
    10921 acgccgtgaa atagggtcaa caccattggt tggctcatcc agcaatagaa tagcaggttt
    10981 gtgaacaaga cagcatgcca gccccaactt ttgtttcatg ccacctgaaa gctttccggc
    11041 taatctatct ttaaacgctt caagattgct gaaattccag agcaccggta atgttttttt
    11101 aatttctttt tcgcgcaatc caaatatttt gccaaagaac atgatgtttt caagtaccgt
    11161 taggtcttca tatagcccaa aacgttgtgg catgtaacca atatgctctt taacattata
    11221 gatagcatta tgatatgggt tatcgttaaa aagtgttgta ccggtatcag gtcgcaaaat
    11281 accagccatg attcgtagca atgtgctttt gccggctcca tcaggcccta caataccaac
    11341 aagctcacct ttttccagtt gtaatgaaac atcgttaact gcaataatgc tgttaaactg
    11401 tttaatgagt cccttaacct gtatcattat tcacctgtta ctataacagc atctgcaggc
    11461 attcctggtt ttaattcttt attgctgtta tcaatagtta tttttacagc aaagactaat
    11521 ttcacgcgtt cctcttttgt ctggatagtt tttggtgtaa actcaggctt attggcaata
    11581 gccaccacct ttccgggaaa gggtttgtca ggataactat cggtatacac aaacgccttt
    11641 tgcccatgct ttacccggcc aatatatctt tcattaacgt atatctttat ccatgctttt
    11701 gttaaatcag ctaacgtcat gatggaactg ccggggaggg caagctcgcc tacctctaaa
    11761 ttccgtgtaa gcacaacacc tgcaacaggt gcatacaaca ccgcctgatc aatagttgcc
    11821 ataacaaagt cataatttcc ttttgcaacg ttgtatgcgg caacagcatt atcaagttcc
    11881 cgctgcgaaa tagagccgct tgcaaaaagc tcacgtgccc gttttaaatt tttttcagca
    11941 ttggtaaggt tagccagtgc tgaaagtttc tgagcgctca gttcatcgta tgctaattta
    12001 acaataagtg ctccctgttt tacctcctcc ccttcatcaa aaggaagctc aacaactctg
    12061 ccggcaattt tagatgctac ctcaacctcg gttacttcaa taacgccgga accttctatg
    12121 cggttgtcgt ttttgccact atatcgcagc atttcaaaat agatggtgac gccaacaaca
    12181 ataaccacta ccacaataac aggaataatg cgtttatttt tcatacattt cctcggtaaa
    12241 aattatttca gtacagtcaa atcccgtgtc ttttttaagt tgggcgatag tacaatagaa
    12301 gtcattgatt gccttgatat aacccatgcg tgcagaggta agctgcaatt ctgcatttaa
    12361 caaatctgaa ttttttataa ctccagcacg atagctttct ttagcaatac gtaaaccttc
    12421 tgaagctgtt tctatatttt tgttttgtgc tatgattgtt tcatatgcag tgataagttt
    12481 gccgtaatct gtccgtacag caataataat actttgttta acctgtttaa ttctttcttc
    12541 aatttcttta agcttttcct tttcttcacg ctccactccc tgtacggtat caacaggtac
    12601 aagcgagccc cagcgatacg ttgctgcaaa tcgtatctgc catgttgttt gccattcttt
    12661 gttaccagta attgtagaca aatcaagaga ttgaccgggg aagggaagtg gaacatcgga
    12721 tgtgttgggt aaaaggtaag ttttaccata gtaccccgca atggaaaatg ttggccacag
    12781 atacaccgat tgggttgctt ctatgctatc ttttaccatg tccttacgta atgacagttg
    12841 cagcacctca ggccggtaca aaagagcctt ttcaaccaac aaattaacaa tggcaactgg
    12901 ctgaggtttt ttaatagtgc tatcaaatcc ttcatatgct atagtatatg ttcctgtttt
    12961 agcaccaaca tggtaattaa aaagatcaag cgctgtgtta tagttatttg ctgcttcaat
    13021 aagtagaggt tcctgacttt gtaattgaac ctgtgcctgc agcagctcaa attttggaac
    13081 ggtaccggta ttaaaaaggt tttgtacaac aagaaggttt gattgtaaca cattgataga
    13141 atttttacgt aatgttataa cctcatcagc cagtataaca tcaaagtagc ttttaatgac
    13201 attgtactca atagttgatg ttattttttt aacatcttct tttgccagtt tgtagttact
    13261 ataactttga gttaatgtat tgtaaaaatt ccccggattt atgccaaact taatctgaat
    13321 aaattttaaa tcatactgcc cgtcagataa acttaaaaat ccattttccg catattgccg
    13381 tatggcatca gcttcagact ctaaggccgg gtaaagcata ccccacacct gataaattat
    13441 ttcattggct tgctttaaac gctgttgcgc tgttaataat tcaaagttgt tttttaaggc
    13501 aatgtcaatt gcctgattta aagttagctt ttgcagagca ttaaccggaa tggaaataaa
    13561 aagtattatt atgagcgata gtaatcgagt aatacatatc attttcataa ttactccttt
    13621 ttcacaatac catgcgctaa aagctgcagc agatttgcaa tatattgttc aacattagat
    13681 tcctgaaaat atttttggta gactgaagtt ccttcaagta tctgccgcat gagattataa
    13741 aagataacag cgccggcaat ggaaatcatg gtatattgcg gatgaatatc ttttagcata
    13801 tttttattgt ttccaagctg gtaaaatttc ccaacaagct ctgttattgg ttcaataatg
    13861 gtaggagcaa ctatcttccg tataaattta ccaccacttg aaagttcacg cataatgatc
    13921 aaaaaatagt gcttatcaag gctgctcatg tgccttgcat aatcttcaat aactatgatt
    13981 ggattgcctg cttctatagc tttttgcaga cttccctgaa cagtatcaaa gatggtttgg
    14041 gctaaatttt ttacaatgtc agcatataac gcttctttgt ttttaaaatg gtagtatatc
    14101 attgctttgt ttatcttagc tttttttgca atacggtcaa cccgtgcacc atcaagacca
    14161 tagttagcaa actctattat agctgcatga ataatcttat ctttacttgt tttcatagta
    14221 actaaccgtt tagttaacaa catactgcta tataccataa aagtcaatat tttattatta
    14281 tttttcccct ttatataatg tgggtaggct ctgaccccat ttttttctca ttttctcatg
    14341 tgtaatccaa ttttcaacgt aatagttatg tacttacgca ggcacagaac ataggctccg
    14401 accccattaa tacccatata tgcgattttt tttacaaaat aaaaaaaaat gcaccatttc
    14461 ttttaatttt ttccatctcc ggcgttttat atagtagaga tttcaattat aataaaaaaa
    14521 cggaggaata ccaatggcac ggaaattgcg catacaactg cccgagcata cctatcacat
    14581 catgagccgc tgtatcgagt ggcgggatat gatgcaggag gagtacttta
//
LOCUS       DAOSOX010000045        14336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4253_length_14336_cov_8.252503, whole genome shotgun sequence.
ACCESSION   DAOSOX010000045 DAOSOX010000000
VERSION     DAOSOX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4253_length_14336_cov_8.252503"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLX22_04970"
     CDS             complement(<1..484)
                     /locus_tag="PLX22_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19281.1"
                     /translation="MIVARNIMIIVLFAVLFIFTFYTSCVQYEFPVYRVVVDPGHGGK
                     ALLPKDYHGDRFDILSMKYLDMFREGAMYRQYYEHIYTYEIAVRIEKILQLTLPDGDF
                     KKFCSIVQKYTNKPFERVCIQSFVSRQPSLNSQMLHKDPNAPYRLFDSVFPDGTLQEG
                     R"
     gene            complement(555..1286)
                     /locus_tag="PLX22_04975"
     CDS             complement(555..1286)
                     /locus_tag="PLX22_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19282.1"
                     /translation="MKKKGLLFILSVALIFSLQLSPIVNAQQAQPQQAEKSEFTEATR
                     PEENYLARFHALERVEYLMKSNIDKIYMLKIIVTNYKDQGWEKDYEACYNGYKKGMDY
                     YYKRDVIYAQVELEKNRKDIYELYKKIAEVYKQRTQALLNECASKILDLSLDERSKSN
                     PDRNKVLFNNTARLRIAYGQYDDAQNMMDDHVYDTAVKHFRVAKSYAILILENIDPEN
                     SKGKYDLDKADNMNRILNQPQTQAK"
     gene            complement(1433..2767)
                     /locus_tag="PLX22_04980"
     CDS             complement(1433..2767)
                     /locus_tag="PLX22_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT19283.1"
                     /translation="MPKKHKQRDDIKAIYTHAIAAVDPYSAVCKFLQRKGNTLIIKGD
                     SNLVIDCDTIDAIYVVGAGKATARMAKAVEDIIGERIVEGIVVVKYGYREDLQYIQQL
                     EAGHPVPDENGLKASQKIIDVLGKAGEHDLIISCISGGGSALLPYPVEPITLAQKQDL
                     TQKLLKSGASIKELNVVRKHVSRTKGGNLALAAYPATVINIMISDVVGDDMDIIASGP
                     FVCDTSTYADALAILEKYKLTGTIAHAIIEHLEKGAHGEIPETPKDENIFKNVHNIIV
                     ASNSIACLAAYKKASELGYTPLFLSSMIEGDTGEAACFHGAIAHEIIKTGNPVQKPAC
                     IISGGETTVVVKGSGLGGRNMEFAMVMATLIENTSIVAASVGTDGTDGPTDAAGAVAD
                     GNTLNKAGRMGLDINDYMNNNDSYHFFERLGDLIKTGPTNTNVMDIRIILVV"
     gene            3076..4359
                     /locus_tag="PLX22_04985"
     CDS             3076..4359
                     /locus_tag="PLX22_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19284.1"
                     /translation="MKKVRRAFFLSCLLMAMATSVVQAKTLLVVYPFCPDDLKNEATI
                     LFELLNKMTIENYSIENTKQIGSIDSFIKLSNEDSLSVIKKTARKRKNDYILFFEMSH
                     SVGQIFIESKLYSNTEGSFIATSFDWAYYHAIADIVKTIETKLSLIFQSKIIKITNVS
                     TLPDSTTNQITIHCETYGDNTSFAFVRSLFREGPYDTIATITTPSFTDNDVVPGIVYW
                     YRVQALYDSVVIDTSQPVSACIQAENKENLDLQKVLSLYKNPEEKPADDEQKETIKKH
                     LELLQPLYINSVKLRIIMQVVKSYVNKGQLIVLRNFDTTVMDTDNNCMYFIKKNSYVV
                     KLESKKPFNVLAASNFDLELLQRLSLNAIALGVPDNYIEITDDEGYTMQLPFYQAIGI
                     ATTYFTEYKHWASNTIMWNTSNKELNAKMKDYTNQ"
     gene            4423..4932
                     /locus_tag="PLX22_04990"
     CDS             4423..4932
                     /locus_tag="PLX22_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19285.1"
                     /translation="MLFKRKKKVLPDVYEFTDDDGKKWLQTSTKDLLSSKELNDIIKE
                     IEKEKEEGEIVIARIDFKKVNKEYYERVVELEDTLKKRTDLLKRIIKESRETIDRKNK
                     KLKELIEYIKKLHLILAYYKLSPEDFNKIDFTKAYAKPSEIPAPQPSIEIKEKVVEYV
                     PVEEQIMQD"
     gene            5340..>6094
                     /locus_tag="PLX22_04995"
     CDS             5340..>6094
                     /locus_tag="PLX22_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT19286.1"
                     /translation="MPGGIAKDSIAEYPPVTIYSCGPTVYSYAHIGNFRTFIFNDFLR
                     RYLKFRGYTVNHAMNITDVDDKTIAGALKEGVTLKEYTDKYTRIFFEDCKTLNIEPVE
                     HYPRATESIDAMIDIIAHLDKKGLVYEKDGSLYFSIAKFHKYGQLSNVTSMDIKAGAR
                     YDADEYTKEDVRDFALWKAPKENELYWETPFGKGRPGWHIECSAMVRKIFGTTIDIHT
                     GGVDLIFPHHENEIAQSEAAYDEPFVRYWLHVE"
     assembly_gap    6095..6194
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6195..6546
                     /locus_tag="PLX22_05000"
     CDS             <6195..6546
                     /locus_tag="PLX22_05000"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT19287.1"
                     /translation="SFIEAVDDDLNIAEGIGTFFDFLHDINTLIDKRMLGKADADTIM
                     HILNHIDTVFGFIFFHGAVTVNKEEIEALIEERNKARKEKNFTQADSIRDELYSKGII
                     VQDTREGTRWLIKS"
     gene            6528..7253
                     /gene="rlmB"
                     /locus_tag="PLX22_05005"
     CDS             6528..7253
                     /gene="rlmB"
                     /locus_tag="PLX22_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT19288.1"
                     /translation="MADQIIAGRKVVLEYLSSIEGDATLYISESAHGRILDVIADKAS
                     SLNIPINRVPRSWFDSHCQPHHQGVALKMSYRPHNTLQNRFHLPDIASSQGVIVACDG
                     ITDPHNIGSIIRTTEALGGNAVIVTKAHAPEITQTIIKASAGATAHIIVHYVANLASF
                     INDAKQSGFWIIGTSDSGTHPIHSIRQYKPAVLIIGSEGSGMRQLTQQLCDITVAIPL
                     RGKVSSLNASVAAGIVLWELLKE"
     gene            7503..8456
                     /locus_tag="PLX22_05010"
     CDS             7503..8456
                     /locus_tag="PLX22_05010"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT19289.1"
                     /translation="MNIRLLFRVCCCSLIFFIFFFCYILISCTGNPSATDVIEITKVN
                     HFITFDDRARGITATASTLPAVVISHETHSSASIQCKTCHHKQYNDERIKTCSFCHKG
                     TMGAEMYHTFCINCHTVKHKGPVTCNQCHKEKSSRLNEYNEIKKIYKKTFAFTENHHS
                     IHSKENIQCIQCHHNTKNNTKPESCSNCHAGHSKMVVLHNFCKDCHKKQGISISCEQC
                     HKGTQTNNIIKYETIILEKTGHRLPPIKFNHKAHIESYNTECIDCHHIGSLQKCSSCH
                     TKKDNKEIINLRSAFHQQCHECHRRSKGPLGCLRCHYEKVL"
     gene            8498..10774
                     /locus_tag="PLX22_05015"
     CDS             8498..10774
                     /locus_tag="PLX22_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19290.1"
                     /translation="MKISEYLKQLVNKDKSITKSIRYALILTVILISLSFLLITCFYT
                     ANQMEQSMVNNYHEILLKQFEFIEYWLENKVEHIEKLSTASIINEYIESKQINKLIEY
                     LHQVIADQGEYYAITIMNDDGDVLCTTNSTFSFTMSAIYNQIKSTNDTAICKTIKIPF
                     KGNSITALPISCPIGKSIHYVKTKSPYIICFVNMDILFDSMRMINVGSHGNVYLIDKD
                     AQVILAANNNKPKNSYFNDYSLQDDRHSKNFKPVNKDGQLIASVKKCLSDFRSGIAQY
                     DNAYNDKVIGLWKWLSYFQYMFLIEISYAELLKPIYHTIIVYVMVSIAFAAIAIFFSI
                     TFSKNIKNKLSLFIKSFGEASVGNLSSEYPVTFNKNNIEIFMKQGETFIPYTSSKHCF
                     FEIGSIAKKFGNDIHCKFILEGQFKSCLQCSIYQSNAANEIDLLGAWFNAFMDKLRNI
                     IEDVSQIIQETFTASDEVSQSTQDFSENSNTLASSSEEIMATMEQLGAGFEEISKSVN
                     QQDVSISTMIATFDSLTEIMNDLNKIIIDAKENSNKFQTKAKEGQDMLQTMHESMTSI
                     QKSSQKIIDIIAIIDTISDHINLLALNAAIEAARAGEAGKGFAVVASEVGKLAEQTAT
                     SLKDIEKLILSSDNEIKNGIVNIQNTVSTIQSIIEGFSMIGTMMYEISNKMEIQNNTK
                     NQFVEEMTKVKDISSIINNSVREQQTASDNVIKSVSIINESTQGIAARSEELAANSIN
                     LKDRISLLQREIQFFKKV"
     gene            complement(10782..12038)
                     /locus_tag="PLX22_05020"
     CDS             complement(10782..12038)
                     /locus_tag="PLX22_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT19291.1"
                     /translation="MEYLTAIRPYIIKRLEQIKKADVLIGIPCFNNEATIANVMTMVS
                     EGLFTYYNDVRPVLFVSDGGSTDDTRDIAQSIELKPWQERIIQIYRGVAGKGSAFRAI
                     FEAAQILDVDACIVVDSDLRSISPDWIKLLLSPVLEKDFEYVAPVYTRYKYDGTITNN
                     IVYNIIRAVFGKRIRQPIGGDFAISKNLAKIYLDKPVWTTDIARFGIDIFMTVNAIVS
                     HVKICQANLGVKIHDAKDPAQSLGPMFVQVIGTLFNLMEGYEPYWKNIERSKPVPTFG
                     DINASEPEPIEVNLERLVNEYKLGFSQFHSLYQEMFSSDVYNALLSSSKLDVIHFSIP
                     VEVWVKVVYELSAIYHTWKINKVRLINLMVPLYFGRIASFINETLSMNSFQAEEEVEK
                     QAQVFENYKPYLIEKWQLQGKDIPFA"
     gene            12259..12834
                     /locus_tag="PLX22_05025"
     CDS             12259..12834
                     /locus_tag="PLX22_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19292.1"
                     /translation="MNYRVVKQFWINTVDNFFNFFYKIVDFLKIWVDVFWAFMDIWYH
                     FFAIFGNLFLYIYYIFLFIIDKSTESGTNILLTSRLPKQVPFRPQKAYVKDSYNPIPA
                     MYGVKNVAETVASSITPMRTTPKGTRTSIVKKLFTIIRDGIDIIVGLFKKPVKAIGNV
                     AARRMKPVKEEPHKTGSLIEEYIKEYEQKRH"
     gene            complement(12847..14133)
                     /locus_tag="PLX22_05030"
     CDS             complement(12847..14133)
                     /locus_tag="PLX22_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOT19293.1"
                     /translation="MEDLKKAYRTIMDDHFPDTMTISFGNQTLIYKKRTWKINDDGQL
                     IEKGLRYGENPGQEAALYELVNGNLVLGDCEFISPGHSLVSGIDESMMLQFGKHPGKI
                     NLTDIDNALNIVKYLMKQPAAVIVKHNNPCGVAYGVTIAQAYNRANRADRIAAFGGCV
                     VCNRAVDKETAQLISQNYLEVLVAPDFEPGVMDILSKKKDLRIVRIQRIDRLEEYKQL
                     RFVDFKSLIDGGIIVQQSPVTIINSKDDLKPAQAEYKGQLYKIDRLPTPREYEDLLFG
                     WFVEQGVTSNSVLYVKDGVTVGIGTGEQDRVGVAEIAVQKAYTKYADMLCFDNYGISY
                     YQLALEVEKGLRKASDKIAIDEATKEAKGGLIGASMVSDAFFPFRDGVDVGIKQGISA
                     IVQPGGSIRDWESIMACNEANPKVAMVFTGQRAFKH"
BASE COUNT         4745 a         2712 c         2411 g         4368 t
ORIGIN      
        1 tcctgccttc ctgcaatgtc ccgtccggga aaacagaatc aaacagtcgg taaggcgcat
       61 tgggatcttt gtgtagcatt tgagaattta atgaaggttg ccggctcaca aatgattgta
      121 tgcagacacg ttcaaacggt ttattggtat atttctgtac tatggaacaa aactttttaa
      181 aatccccatc aggtaacgtt aattgtaaaa ttttttctat acgtacagca atctcgtatg
      241 tgtatatatg ttcataatac tgtctataca tagctccctc acggaacata tcaagatatt
      301 tcatggacaa gatatcaaat ctgtcaccat gataatcttt agggagtaga gctttacctc
      361 catgtccggg atcaacaaca actcgataga caggaaactc atactgaaca caagaagtat
      421 aaaatgtaaa aataaataga acagcaaaca acacgattat cattatgttt ctggctacaa
      481 tcatcagcaa tactatacca tcataaaaaa ggggtaatgc attacccctt ttttattatt
      541 atcgttactt gtaactattt tgcctgtgtc tgaggttgat tcagaattcg gttcatatta
      601 tctgctttgt ccagatcgta cttccctttt gaattttcag gatcaatatt ttccagaatg
      661 agtattgcat acgattttgc aaccctgaaa tgttttacgg ctgtatcata gacatgatca
      721 tccatcatat tttgtgcatc atcatattgt ccatatgcaa tccgcaaacg agcagtattg
      781 ttaaatagta ccttattcct gtcaggattt gatttgctcc gctcatcaag cgaaagatca
      841 agaatcttgc ttgcacattc gttaagcaat gcctgggttc tttgtttata aacttcagct
      901 atctttttgt ataattcata gatgtcttta cggtttttct ccagctccac ctgtgcatag
      961 attacatccc gcttataata ataatccata ccttttttat agccattata gcatgcttca
     1021 taatcttttt cccagccctg atctttatag ttggtcacaa ttatttttag catatatatt
     1081 ttatcaatat ttgatttcat taagtattcc acacgttcta acgcatgaaa ccttgccaga
     1141 taattttctt ccggacgtgt tgcttcggta aattctgatt tttcagcctg ctgcggctga
     1201 gcctgctgcg catttactat tggtgaaagc tgaagtgaaa atatcaatgc aaccgataaa
     1261 ataaaaagca aacctttctt tttcattgta ttactccttt aattgataat tgaaattagc
     1321 atgcattaaa gtattggcat ccttgcaaaa ccttatatta aaaattaatg atatcaagca
     1381 accttgtgtt tagcaagtat tttttttatt ttttttatca aataaaaaaa ttttacacca
     1441 ccagtataat cctgatatcc atgacattgg tatttgttgg tcctgttttt attaaatccc
     1501 ctaatcgttc aaaaaaatga tacgagtcat tgttgttcat gtaatcattg atatcaagcc
     1561 ccattcttcc tgccttattc agcgtattgc catctgcgac ggcacctgct gcatcggttg
     1621 ggccatccgt tccatcagtt ccaacactgg cagcaacaat cgaagtattt tcaataagcg
     1681 ttgccatcac catggcaaac tccatattgc ggccacccaa accgctaccc tttaccacca
     1741 cggttgtttc accgccactt ataatacagg caggcttttg cactggattg ccagttttaa
     1801 taatctcatg agctatcgca ccatgaaagc acgcagcctc cccggtatcg ccctcaatca
     1861 ttgatgacaa aaacagcggt gtatagccca attcgctggc ttttttatac gctgccaggc
     1921 aggcaatgct atttgatgca acaataatat tgtgaacatt tttaaatata ttttcatcct
     1981 tgggtgtctc gggaatttca ccatgtgccc ctttttcaag gtgctctata atggcatggg
     2041 cgatagttcc tgttaattta tatttttcta aaatagcgag tgcatcagca tacgtgctgg
     2101 tatcgcaaac aaaaggaccc gatgcgatga tatccatatc atcaccaacc acatccgata
     2161 tcataatatt gattaccgtt gccgggtaag ccgccagtgc caggttgccg ccttttgtgc
     2221 gcgatacatg tttgcgtacc acattcaatt ctttaatgga tgctccgctc ttcaaaagtt
     2281 tttgcgttaa atcctgtttc tgggctaatg taattggttc tactggatag ggcagaagtg
     2341 ccgaccctcc tcctgaaata catgaaataa taagatcatg ctcacctgct tttcccagta
     2401 catctataat cttttgcgat gccttaagtc cattctcatc aggtacaggg tgccccgctt
     2461 caagctgctg aatgtactgc aaatcttcac ggtatccata ttttacaaca acaataccct
     2521 caacaatacg ctctcctatg atatcttcaa cagcctttgc catccttgct gttgctttac
     2581 cagcacctac aacatagatt gcatcaatgg tatcacaatc aatgaccagg ttactatcgc
     2641 ccttaataat taatgtattt cccttccgtt gtaaaaattt gcaaacggca ctatagggat
     2701 caactgctgc aatagcatgg gtatatatgg cttttatatc gtcacgctgt ttatgttttt
     2761 taggcatggc aaatcctcca aaatagaatt attagcctca gccccggcct tgttttcctc
     2821 tctttattta aggagaggga ataagggtga ggtatgtata cttttcatcc attcttagtg
     2881 actaaacatc aaccattggt aattccctga aaaatgattg atgagctcat cactattttt
     2941 atacaacatg ccataaaatt tacttgcata caggcattac cattgagtat attgagtatg
     3001 atagttgcat aaccaattgt caaatatttt acaatgctat cagcaaatag caattataaa
     3061 aaattaggta tttatatgaa gaaagtaaga agggcatttt tcttatcatg tttattgatg
     3121 gcaatggcaa caagcgttgt acaggctaaa acacttttag ttgtttaccc tttttgccct
     3181 gatgacttaa aaaatgaagc taccatactt tttgaattat tgaataaaat gactattgaa
     3241 aactattcaa tagagaacac aaagcaaata ggttcaatag attcattcat aaaattaagt
     3301 aacgaggatt cgctttccgt aataaaaaag acagccagaa aaagaaaaaa tgattacata
     3361 cttttttttg aaatgtcgca ttcagttggg cagatcttta ttgaatcaaa gttatattct
     3421 aacacggaag gatcatttat tgcaacatca tttgactggg cttattacca tgccattgct
     3481 gatattgtta aaactatcga aacaaaatta tcattgattt ttcagtcaaa aatcattaaa
     3541 ataaccaatg tctctacgct acctgacagc accaccaatc agataaccat acattgtgaa
     3601 acctatggcg acaatacttc gtttgcattt gttcgttctc tttttcgtga aggaccatat
     3661 gatactattg ccacaataac aactccatca tttactgata acgatgttgt accgggaatt
     3721 gtttactggt atcgcgtcca ggctctttac gatagtgtcg tgattgacac ttcacagccc
     3781 gtatccgcct gtatacaggc tgaaaacaaa gaaaatctgg accttcaaaa ggtactatcg
     3841 ctctataaaa atcctgaaga aaagccagca gatgatgagc aaaaagaaac tatcaaaaaa
     3901 catttagagt tattacagcc actgtatata aactcggtta aactgcgcat cattatgcag
     3961 gtagtaaaat catacgttaa caaaggacaa ctcattgtgt taaggaattt tgatacaact
     4021 gtcatggata cggataataa ttgcatgtat ttcattaaaa aaaatagcta tgttgtaaag
     4081 ctggaatcta aaaaaccttt taatgtatta gctgcaagca attttgattt agaactttta
     4141 caacggttat cacttaatgc gattgcgctt ggtgttcctg ataattacat tgaaattacc
     4201 gatgatgaag ggtatactat gcaactgcca ttttatcagg caattggtat tgcaacaacc
     4261 tatttcacag aatacaaaca ttgggcttcc aataccatta tgtggaatac gtctaataaa
     4321 gagttaaatg caaaaatgaa agattataca aaccagtgat ttgattaaat aaaatgtata
     4381 aatagtcgat agtataataa ataataatct cacaaaatag ttatgttatt taaacgtaaa
     4441 aagaaagtat tgcctgatgt ttatgagttt accgatgacg atggcaaaaa atggctgcag
     4501 acatcgacaa aagatctttt gtcttcaaaa gaacttaatg atattattaa agagatagaa
     4561 aaagaaaaag aggaaggtga aattgttatt gcccgtattg actttaaaaa agtcaacaaa
     4621 gagtattatg aacgtgttgt tgaactggaa gatacgttaa aaaaaagaac tgatttgtta
     4681 aaacgtatca ttaaggaatc acgtgaaacc attgaccgca agaacaaaaa acttaaagaa
     4741 cttattgaat atattaaaaa actccatctt atattagcat actataaact ttcgccggaa
     4801 gattttaata agattgattt taccaaagct tatgcaaaac cctctgaaat accagccccg
     4861 cagccttcta ttgaaataaa agaaaaagtt gttgagtacg tccccgtaga agaacagata
     4921 atgcaggatt aatttagtta ttgacagcta tctttaccag ctatagttga catactatag
     4981 caattgaaag gctatcgacg gcatgcctgt caatggaaaa tgaatgttaa aactattgta
     5041 aacatttacc aatgacgcac actgtaacgc aaaaacagat taccaaacta cccggcatga
     5101 acactatgta taggtataaa aggctgggat tacccataaa aattaatcca actttgctat
     5161 tttttggaaa aattaaatct atgtttttag aattgaaact atatgacatc ttttttagaa
     5221 aacaaaataa ttagagcata ctgtgcacaa cagtatgtat acatactgtg cagaataatg
     5281 gaggctatat attggcgctg aaaatattta atacattaac acgacgttta gagccattca
     5341 tccccggtgg cattgcaaaa gatagtattg cagaatatcc ccctgttacc atatattcct
     5401 gcgggccaac ggtgtacagt tatgctcaca ttggcaattt ccgtacattt atatttaatg
     5461 attttttgcg gcgctatctt aaattcagag gatatacagt aaaccatgca atgaatatta
     5521 ctgatgttga tgataagaca atagccggag ctcttaaaga aggggtgaca ttaaaagaat
     5581 acaccgacaa gtatacacgg atattttttg aggattgtaa aacgttaaac atagaacctg
     5641 ttgaacatta cccccgtgcc acagaatcta ttgacgccat gatagatatt atagcacatc
     5701 ttgataaaaa ggggcttgtt tatgaaaagg atggctcact ttatttcagc attgcaaagt
     5761 ttcataaata tggacagttg agcaatgtca ccagtatgga tatcaaagca ggtgcgcgat
     5821 acgatgccga tgaatatacc aaggaggatg ttcgtgattt tgcactgtgg aaagctccta
     5881 aagaaaatga gctatactgg gaaacccctt ttggtaaagg cagacctggg tggcatattg
     5941 aatgctcggc tatggtgcgc aaaatttttg gcaccaccat tgacatacat accggtggtg
     6001 tagatttaat atttcctcat catgaaaatg aaattgccca gagcgaagca gcgtatgatg
     6061 agccttttgt acgttactgg ctccatgttg agcannnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnatcatt tattgaagct gttgatgatg accttaatat tgcagaaggg
     6241 attggtacat tttttgactt tttacatgat atcaacacat tgatagataa acgcatgctg
     6301 ggaaaagctg acgctgatac tataatgcat atccttaacc atattgacac cgtttttgga
     6361 tttatatttt tccatggcgc cgtaacagta aacaaagaag aaattgaagc tcttattgaa
     6421 gagcggaata aagcccgtaa agaaaaaaat tttacacaag ccgatagtat ccgtgatgaa
     6481 ctgtatagca aagggattat agtacaggat accagagagg ggacgcggtg gctgatcaaa
     6541 tcatagcagg acgcaaggtt gtccttgaat atctatcatc aattgaaggt gatgcaaccc
     6601 tctacatttc cgaaagtgca catggcagga tacttgatgt tattgcagat aaagcttcca
     6661 gtctgaatat ccccattaac agggttccca gaagctggtt tgatagccac tgccagcctc
     6721 atcatcaggg agtagctctt aagatgagct atcgcccaca caatacacta cagaatagat
     6781 ttcatttgcc cgatattgcc agttcacagg gggttattgt tgcctgcgat ggcattaccg
     6841 atccccataa tattggctcc ataatacgca ctaccgaagc gctgggtggc aatgctgtca
     6901 ttgtaacaaa agcccatgcc cctgaaataa cgcaaaccat cataaaagca tcagcaggtg
     6961 caactgctca tattattgtt cattatgttg ccaatttagc aagctttata aacgatgcaa
     7021 aacaatctgg cttctggatt attggcacat ccgacagcgg tacgcatccc atccatagca
     7081 tacggcagta taagccggcg gtacttatta ttggcagtga agggagcggc atgcgccagc
     7141 ttacacaaca actgtgcgat ataacggttg caataccatt acgaggaaag gtttcatcac
     7201 tcaacgcatc ggtggcggct ggcattgtgt tgtgggagtt attgaaggag taaaaaatat
     7261 gtatacacaa atttgattac gatagcaact atatatgcaa cattttttta attatattaa
     7321 tttacttctt gacaactacc gacgattaat agtatataga ttaatgttta atacgttctt
     7381 tgatatttgg ttttaaaaca atgaatgcaa agattgtcta ttgtgttaac atacaaaact
     7441 attgagcaat agagagaaat atagaagatt ttgtaatgtt atagcgtgaa ggaaatgaag
     7501 ggatgaacat acgattgtta tttcgtgttt gttgttgctc attaatattt tttatattct
     7561 ttttttgtta tatactcata tcatgtactg gtaatccttc agcaaccgat gttatagaaa
     7621 taacaaaagt aaatcatttt attacattcg atgacagggc aaggggaata accgctactg
     7681 catcaaccct tccagcagtt gtgatatctc atgaaacaca ttccagcgca tcaatacaat
     7741 gcaaaacatg ccaccataaa caatataatg acgaacgtat taagacatgc tccttttgcc
     7801 acaagggtac catgggtgca gagatgtacc ataccttttg tattaactgt catacagtaa
     7861 agcataaagg tcctgtaacc tgtaatcaat gccataaaga aaaaagcagc aggctgaatg
     7921 aatataatga aattaagaaa atatataaaa aaacctttgc gttcactgag aaccatcatt
     7981 ctatccatag taaagaaaac atccaatgta tacaatgtca tcataataca aaaaacaaca
     8041 caaaaccgga atcatgtagt aattgccatg caggacattc aaaaatggtt gttttgcata
     8101 atttttgcaa agattgtcat aagaaacaag gtatttcaat tagctgtgaa caatgccata
     8161 agggaactca aaccaacaac attataaaat atgaaactat tatacttgaa aaaacggggc
     8221 atagattacc acctataaaa tttaaccaca aagcccatat agaatcgtat aacaccgaat
     8281 gcattgactg tcatcatata ggttcattac aaaaatgctc atcctgtcat acaaaaaagg
     8341 ataacaagga aataatcaat ttgcgttcag catttcatca acaatgtcat gaatgccata
     8401 gaagaagcaa aggtccattg ggatgtttac gctgtcatta tgaaaaagtt ttatagagaa
     8461 caaattgaac aaaatttatt attaattgaa atggtatatg aagatatcag aatatttaaa
     8521 acaactggtt aataaggata aaagtataac aaaatctatc agatatgcgc ttatacttac
     8581 cgttatattg atatcgttat cttttttgtt aattacatgc ttttatacag ctaatcagat
     8641 ggaacaaagc atggtaaata attaccatga aatattgcta aaacaatttg aatttattga
     8701 atattggttg gaaaacaaag tagagcatat tgaaaaatta tcaacggcat ctatcattaa
     8761 tgaatatata gaatccaaac aaataaataa actcattgaa tatttgcatc aggttattgc
     8821 tgatcagggt gaatactacg ccattaccat tatgaatgac gatggggatg tactgtgtac
     8881 caccaatagc acattttcat ttaccatgag tgctatctac aatcaaataa aaagtaccaa
     8941 tgatactgca atttgtaaaa caataaaaat accgttcaaa ggtaatagta taactgcatt
     9001 gcctattagt tgtcctattg gcaaatctat acattatgtt aaaacaaaat caccttatat
     9061 aatttgtttt gtcaatatgg atatactatt tgattcaatg aggatgataa atgtgggtag
     9121 ccatggcaat gtttatctca ttgataagga tgcacaagta atcctggcag caaataacaa
     9181 taaaccaaaa aattcatatt ttaacgatta ttctttgcag gatgataggc acagtaaaaa
     9241 ttttaagcct gtcaataaag atgggcagct aattgccagt gtaaaaaaat gcctttctga
     9301 ttttagatct ggcattgcac agtatgataa tgcatacaat gataaggtca taggattatg
     9361 gaaatggctt tcatattttc agtacatgtt tttgattgaa atttcttatg ctgaattgct
     9421 aaagcctata taccatacaa taattgtata tgttatggtt agcatagctt ttgcagcaat
     9481 agccatattt tttagcatta ccttttccaa aaatattaaa aataaactat cactctttat
     9541 taaaagtttt ggcgaagcaa gtgttggaaa cctttcatct gaatatccag taacattcaa
     9601 taaaaacaat attgaaattt ttatgaaaca aggtgaaacc tttatcccct atacatcttc
     9661 aaaacattgt ttttttgaaa taggcagtat tgctaaaaag tttggtaacg acatacattg
     9721 caaatttata ttggaaggac aattcaaatc atgcttgcaa tgctcaatat accaaagtaa
     9781 tgcagcaaat gaaatagacc tactgggtgc atggtttaat gcattcatgg ataaattgcg
     9841 aaatattata gaagatgtaa gtcaaataat tcaggaaacc tttacagcat cagatgaagt
     9901 ttctcaatca acacaagatt tttctgaaaa cagcaatacg ttagcctctt cttcagaaga
     9961 aataatggca accatggaac aattgggggc aggatttgaa gaaatcagca aaagtgttaa
    10021 tcagcaagat gtaagtatct caaccatgat agctacgttt gattcattaa ctgaaataat
    10081 gaatgattta aataaaatta taattgatgc aaaggaaaac agcaataaat ttcagacaaa
    10141 agcaaaagaa ggtcaggata tgctccaaac catgcatgaa tctatgacct ccatacagaa
    10201 aagctcgcaa aaaataattg atattatagc aattattgac actatttctg atcatattaa
    10261 tctactggca cttaatgcag ccattgaagc agcccgtgcc ggtgaggctg gcaaagggtt
    10321 tgctgttgtt gcatcagagg ttggaaaatt agcagagcaa accgctacaa gtttaaaaga
    10381 tatagaaaaa ttgattttat ccagcgataa cgaaataaaa aatggtattg tcaatataca
    10441 gaatacggtt tcaacaattc aaagcattat tgaaggattt tcaatgattg gtacaatgat
    10501 gtacgaaata tcaaataaaa tggaaataca aaataacact aagaatcagt ttgttgaaga
    10561 gatgactaaa gttaaagata tttcttcaat catcaataat tccgtaaggg aacagcaaac
    10621 tgcatcagac aatgtaataa aatctgtcag tatcattaat gaatcaaccc agggaattgc
    10681 tgctcgttca gaagagctgg ccgcaaattc tataaattta aaagatagga tatcattatt
    10741 acaacgtgaa atacaatttt ttaaaaaagt ataatatgct attacgcaaa tggtatatcc
    10801 ttaccctgta actgccactt ttctattaaa tacggtttat agttttcaaa aacctgcgcc
    10861 tgtttttcca cctcttcctc agcctgaaac gaattcattg acaatgtttc attgataaat
    10921 gaagcaatcc tgccaaaata taatggcacc ataaggttta ttaaacgtac tttatttatc
    10981 ttccatgtat gataaatagc agaaagttca tacacaacct taacccagac ttcaacaggt
    11041 attgaaaaat gtataacatc aagctttgat gaagacaaca gcgcattata cacatcactt
    11101 gaaaacatct cttgatacaa tgaatgaaat tgtgaaaacc ctaacttgta ctcattcacc
    11161 agcctttcta aattaacttc tattggttca ggctcactag cattgatatc gccaaaggtt
    11221 ggcacaggtt tactgcgttc aatatttttc cagtacggtt catacccttc cataagattg
    11281 aaaagcgttc cgataacctg aacaaacata ggaccaagtg attgcgcagg atcttttgca
    11341 tcatgaattt ttacaccaag atttgcctga catatcttca catgactcac aatagcattg
    11401 acagtcatga atatatcaat accaaaacgg gctatatcgg ttgtccagac cggcttgtct
    11461 aaataaattt ttgcaagatt ctttgagatt gcaaagtcac caccaattgg ctgtcgtatc
    11521 cttttcccaa acactgcacg aataatgttg tagacaatgt tgttggtgat agttccatca
    11581 tatttatacc gtgtatacac cggtgcaacg tactcaaagt ctttttcaag tactggcgat
    11641 aacaacagtt taatccaatc aggagatata ctgcgtaaat ctgaatcaac aactatgcac
    11701 gcatctacat caagtatctg tgccgcctca aaaattgctc tgaatgctga acctttcccg
    11761 gcaaccccac gataaatctg tattatccgt tcctgccatg gcttaagttc tattgattga
    11821 gcgatatcgc gtgtatcatc agtagaacca ccatcagaaa caaacagtac cggccttaca
    11881 tcattataat atgtaaacag accttcgctt accattgtca taacattggc tatcgttgct
    11941 tcattgttaa aacagggaat gccaataagt acatccgctt tttttatttg ttctaacctt
    12001 ttaattatat atggccgtat agctgttaaa tattccatag tgtcaagtat ataatataca
    12061 acactattat caagattttt taaataaaat acacttataa aatacaatta atttaaaaaa
    12121 ctatcgaaaa caagctataa acaaaatagt acaaataata tagcagcctg caattgcaat
    12181 aatttaattg actcttttta cagggaattt actatatgtt tatagtcttc atttattagt
    12241 attgctgagg ttactgttat gaactatcgt gttgtaaagc aattctggat taataccgtt
    12301 gataattttt tcaacttctt ctataagatt gtagattttt taaaaatctg ggttgatgtc
    12361 ttctgggcat tcatggatat atggtatcac ttttttgcca tctttggaaa tttatttctc
    12421 tatatatatt atatattcct gtttattatt gataagtcaa ccgaatcagg gacaaacata
    12481 ttactaacaa gcagattgcc aaaacaggtc ccctttagac cacaaaaagc gtatgttaag
    12541 gatagctaca atccaattcc agcaatgtat ggtgtaaaaa atgtagcaga gacagttgcc
    12601 tcaagcatta ctcctatgcg tactactcca aaaggcacca gaacatctat tgtaaaaaaa
    12661 ttgttcacta taattcgtga tggtatagat attattgttg gactgttcaa gaaacccgtt
    12721 aaagccatag gtaatgttgc agcccggcgc atgaaaccgg taaaagaaga gccacataaa
    12781 acaggaagcc ttatagaaga atatataaag gaatatgagc aaaagcgtca ttgaacgcct
    12841 ttgcctttaa tgtttaaacg cacgctgccc ggtaaaaacc atagccactt ttggattagc
    12901 ttcattgcat gccattatac tctcccaatc gcggattgaa ccgccgggct gcacaatggc
    12961 agagataccc tgctttatac ccacgtctac accatccctg aatgggaaga atgcatccga
    13021 aaccatgctt gctccaatga gtccgccctt tgcttctttt gtagcttcat caattgctat
    13081 tttatcgctt gctttacgca atcctttttc aacctcaagt gctaactgat agtacgaaat
    13141 tccatagtta tcaaaacata gcatatcggc atatttggta taggctttct gcacggcaat
    13201 ttcggctacg cctaccctat cctgctcgcc ggtacctatg ccaacggtta ccccatcctt
    13261 aacatacagt accgaatttg aggtaacacc ctgctccaca aaccagccaa acaaaaggtc
    13321 ttcatattcc cggggggttg gcaaacggtc aattttataa agctgcccct tatattctgc
    13381 ctgtgcaggt tttaaatcat ctttagaatt aatgatagta accggtgatt gctgaactat
    13441 gatgccgcca tcaatcagcg atttaaagtc aacaaagcgc aattgcttat actcttcaag
    13501 cctgtcaata cgctgaatcc ttacaatgcg aaggtccttc ttctttgaca atatatccat
    13561 gactccgggc tcaaaatccg gagcaaccag cacctccaga taattttgtg aaataagctg
    13621 tgcagtttct ttatctacag ctctgttgca gacaacacat ccaccaaaag ctgcaatacg
    13681 gtcagccctg tttgccctgt tatatgcctg ggcgatagtt actccataag caacaccaca
    13741 gggattattg tgcttaacga taacagcagc tggttgcttc attaaatatt ttactatatt
    13801 cagagcgtta tcaatatcgg taaggttaat cttgccggga tgcttcccaa actgcagcat
    13861 catcgattca tcaatgccac ttaccagcga atgaccgggg cttatgaatt cacaatcccc
    13921 cagcacaaga ttgccattaa caagctcata gagtgctgct tcctgcccgg gattttcacc
    13981 ataccgcaat cccttttcaa tcaactgccc atcatcattg atcttccagg ttcgtttttt
    14041 atatatcagc gtttgattcc caaagctaat ggtcatggta tccggaaaat ggtcatccat
    14101 gatggttcga tatgcttttt ttaagtcttc catatttgaa aatctccata ctatctataa
    14161 attgcattca aaatcttaca ctgcttcacc atatcgcttc aggcactcat tttgtaaagt
    14221 taaaattcta tcccgtaaat cattcattgc cttctcgatg gtattaatag tatattcgtc
    14281 tgttggcatc tcctccccta tcataacata tacttttcca gggttaatat ccccgg
//
LOCUS       DAOSOX010000046        14246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4294_length_14246_cov_9.274117, whole genome shotgun sequence.
ACCESSION   DAOSOX010000046 DAOSOX010000000
VERSION     DAOSOX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_14246_cov_9.274117"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLX22_05035"
     CDS             <1..915
                     /locus_tag="PLX22_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19294.1"
                     /translation="PYRLFDSVFPDGTLQEGRISYINSLHPHMVVSIHFSLNASRYYR
                     GMNAVIAAPYSFMYKGLQYLQGNGGNRSFFYNSNYADWFTETEDKSSFFWYCNDVIQY
                     FMGYRMTNNYQPDIEAFRGYRYNMVQWIYNDPPGWAHIAKYHPAKTAYADNVKQFIPG
                     NAFFKREQSMYEKYRRDGGYEGYGGDNLYASNEIIRYVLYNLYLQGVRHKEQRLAPPY
                     ISIWSIPLHVNAINAYMELGYLARPYTRYILDNHLDDMAEGIAAGIYSLFAGIEVSNK
                     IPCAPYGKKIDLKKYTINKNNNYFTIVR"
     gene            937..2202
                     /locus_tag="PLX22_05040"
     CDS             937..2202
                     /locus_tag="PLX22_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT19295.1"
                     /translation="MKKLSIIAALIFFQTLVYGDNISQRFIAAADKAGKAVVSIQVFV
                     VEKGHYQKVGYASGTIISSKGLVVTNYHVVAKGTVYQVNLCDGTECDVEPFADGSLFK
                     ADEKTDIALLKVKHSAGLPLTPIEFEDKKVFPGQWVMAIGNPFGLQQSVTAGVVSSLG
                     RSDIGFADIEDYIQTDVPINPGNSGGPLISLDGKLIGINTAIRTQSGGYQGISFAIPA
                     FIVKQVVGELVQYGRVRRGWLGFLVQEKMINNNSPVHVEIISIVDGSPAYKAGIEVGD
                     IVREVDGIPIKKISDLMKAIHSKQIGSLVNIVIARDGQLLNFDFVLQEKSKISRVNYW
                     INVLQQSYGITIEDTLYGAVITGVNQFGIAYNVLKKGDIIQSINDKQVTSLDDIIAIV
                     KKTKGTIENCVVLRSGKVIRCYFNLDGTE"
     gene            2206..2643
                     /locus_tag="PLX22_05045"
     CDS             2206..2643
                     /locus_tag="PLX22_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19296.1"
                     /translation="MNKRIILFIIAAFMSMCIQSFSDEKKAEVILDNEFYTSLQNSDA
                     IKRIRTFDMLKDVIVKGTGNVVKVEVADVLKKKFRIVLSLNLHGIQILYYCYTDQDEY
                     FDLLQPDDLFEFSGQFVVATPLNVRFDTFIIDVLLEEGAVVVH"
     gene            complement(2646..3263)
                     /locus_tag="PLX22_05050"
     CDS             complement(2646..3263)
                     /locus_tag="PLX22_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19297.1"
                     /translation="MKNIAKTADIKTNTPVRVYKKGYGYALLTVIENNELFLLCKSSE
                     DFLSHVKENDTVECYLWPYPDGSYDFNTTILGKINAPFPIILLQHTDTIVYNPSRRCL
                     KAQVNIPFTFFTFSINTSRSFSSEEIQWHEGIVKELTDREALLQTTTPLEHYVKGHLT
                     LGTKKIDITGKVTASDDTLYDITFVGLDDADRISILDYVFTVYRE"
     gene            3591..3884
                     /gene="rpsF"
                     /locus_tag="PLX22_05055"
     CDS             3591..3884
                     /gene="rpsF"
                     /locus_tag="PLX22_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT19298.1"
                     /translation="MNKYELIVILRPTDKLDILKEKLRDILKKNQVVIESDEPWGVRA
                     MAYAIDRANDGYYEYFIVQIDPAQISAIIAEFRLQPDILRYLFVRLPNLSKTA"
     gene            3891..4313
                     /locus_tag="PLX22_05060"
     CDS             3891..4313
                     /locus_tag="PLX22_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT19299.1"
                     /translation="MPGDLNKVILVGRIVRDPELRYTPGGAAVVSFSIANNRIYVKNN
                     EKQEVVSFFNCVAWGKLAETVANYCKKGQQLALEGRLQQRSWDDKDGNKRSTVEVVAE
                     SLQFLGGKPHAETSPSVDSMETGDMGSTEPFSDDDIPF"
     gene            4350..4697
                     /gene="rpsR"
                     /locus_tag="PLX22_05065"
     CDS             4350..4697
                     /gene="rpsR"
                     /locus_tag="PLX22_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT19300.1"
                     /translation="MSEEINQGETIQAQENIIPEDTKSSEVARHSEDKALSAAVQVQR
                     VPKFKKKVCRFCEDPNVAIDYKNPDLLVRFITDRGKILPRRITGTCAKHQRHLARAIK
                     RARTIALLPFVVK"
     gene            4713..5561
                     /locus_tag="PLX22_05070"
     CDS             4713..5561
                     /locus_tag="PLX22_05070"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOT19301.1"
                     /translation="MELIVHLIAGIALCVITAIVVYKLKYKALAIALVMVLSALFLGL
                     SYFYNEMIGLELIITGAVFGYVAKRFSFQRYFITSVTIVWLLLLIIHYILYFVKGIDA
                     ISGNQKIIEEIINASSIPVTEKQVLSQQLEDFLPLMRVLMPFSYFFSSAAISILVYVF
                     VFAFFVRFKFLQTEDNTISLFRVDEWVVFLLIASWLVVLLGKELQTVWAIALNIGLVL
                     CVVYTIQGISVILFLFEKKQWPKLYGFLILFVTILLGFQVMFFVITMLMGIGIIDFWM
                     NFRKIA"
     gene            5587..6030
                     /gene="rplI"
                     /locus_tag="PLX22_05075"
     CDS             5587..6030
                     /gene="rplI"
                     /locus_tag="PLX22_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT19302.1"
                     /translation="MRVILQKDVQNLGEAGDIKEVAEGFARNFLLPKKLVIPYNESSA
                     KAIEHQKKLVKIKKEKRKKEGQKLAEALSSVALTIGAQVGEDDKLFGSVTAIDIADKL
                     LQMGYTIDKRKIVLPEPIKKAGEYEVKIKLEEGLTATIKVSVVKE"
     gene            6035..7369
                     /gene="dnaB"
                     /locus_tag="PLX22_05080"
     CDS             6035..7369
                     /gene="dnaB"
                     /locus_tag="PLX22_05080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT19303.1"
                     /translation="MLDVGMPPHDTEAEAACLSSILLSKDALLKVIEILQVEDFYIDA
                     HRMIYEAIRELEIKSVPIDLITIKQRLIDKKQFEKVGEDQALVNIYRTVSTSANAEYY
                     ANRVKELSLRRKLIETCTTIAERSYDTTLDTKVLLDDAEKDIFLITQRRITSDFISLA
                     NVLKETLENIGHMYETKRPVTGIAGGFTDLDAMLTGFHPSELIIIAARPSMGKTALAL
                     NIANHVALREKQPTLFFSLEMPATQLATRILCIESLIDMQHVRTGQISNEQLRKLVDI
                     SGRLEKSPMFIDDTPGLSIFELRAKARRFAQKQKVGLIVVDYMQLIVANSRLERHQQI
                     AEISNALKQLARELSVPVIALSQLSRAVESRTDQMPQLSDLRESGAIEQDADVVLFIY
                     REEKVKKETERLGIADIIVAKQRNGPTGRIELRFWDKYMKFGTVEKFREEAE"
     gene            7371..8156
                     /locus_tag="PLX22_05085"
     CDS             7371..8156
                     /locus_tag="PLX22_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19304.1"
                     /translation="MNNAEIIFLRPVMKFFESVGNHTLFFAQTVKWIFKPPFDTKNIL
                     LQMLEIGYKSLPVTCITLFFTGMVMALQIGRAMDSLMSGSSIFIGSAVSIAMFRELCP
                     VLTALLLSGRVGSAIAAEIGTMRVTEQIDALITLDANPIQYLSVPRFMACLVMTPVLV
                     VITDVVGSLGGAFIAYFNLGITIDKYIENVFNYLQVMDFLTGLLKSVFFGIEIAIIAC
                     YQGFNTFGGAEGVGKSTIRAVVASSMTILISDYFLTYLLQFIE"
     gene            8173..8817
                     /locus_tag="PLX22_05090"
     CDS             8173..8817
                     /locus_tag="PLX22_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOT19305.1"
                     /translation="MKSLKKRKEEFIKLLQKNRIPKRIVDAFAIVNQEDFFDEIYSDA
                     LYGTQPIPVAPGKTGDEPLTLARMIHYCGIKKDFHVLEIGTGSGYSTAIMAHLAQSVT
                     TVEIDEELALLAKKRFSRLKLTNIRVFAGDGTTIDESSGIFNAAVIFAGCTKRPLMLL
                     HVMKQDGVVVFPMGPAHQQQIALMLNHRDEKVEGVFPVVFKEFCTFMPIAGIYG"
     gene            complement(8818..9162)
                     /locus_tag="PLX22_05095"
     CDS             complement(8818..9162)
                     /locus_tag="PLX22_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19306.1"
                     /translation="MVEIILYILSFGAGIALFLYMLSLYHHLQKKTSQSAKKETNKAA
                     TVMPAKTVDKNFTPASMKERTCPVCGSKLSRYEALYASKIVKDSTKILIYGCRYCYKP
                     DEDPDKIKKSDI"
     gene            complement(9168..9890)
                     /locus_tag="PLX22_05100"
     CDS             complement(9168..9890)
                     /locus_tag="PLX22_05100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19307.1"
                     /translation="MNEYHHLAPYYDYMLQHVNYNEWYRYLRNVMCKYISNPRTVLEL
                     GCGTGKFGPKFSMDDYEIYGMDKSIAMLSIAKTRANKNFHIFCGDITCFALSKTVDFI
                     FSVHDTFNYLLTYDDFAKALRCAYNCMSYNSIFLFDITTQYNIMKNFHRRLFAYTVKG
                     TDITWYNEYDEKSKMVYTKLTFATQSRTIIEEHLQRIYTIDEIIPIIKKCGLLVVDIA
                     GDYTMKPVTDHTIMINVITKRA"
     gene            complement(9887..11017)
                     /locus_tag="PLX22_05105"
     CDS             complement(9887..11017)
                     /locus_tag="PLX22_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT19308.1"
                     /translation="MHDSSWISLNGGSTITTPHGAVTLFAHHHDSDSFPITTAQLYLD
                     DYCRIHHIEPLHLHECIFFDIETTSLTSGTGNYAFLLGMGYFANNVFTVEQLFMREYA
                     DEPALLYYFADRVNNYKAIVTFNGKTFDIPIIKTRFRINRIPGFPVSMPVIDLLKPAR
                     SIFKSIYASCSLKSLEELLLDVTRTDDIPGYLIPDVYFTYQQTGFDPRIINVIEHNRI
                     DITSMVLLLVFFNTLYQMLHAKQFHQVPSNLYTNIAKHLYSRNIQLFVELVEYAGFAL
                     LHNEHSLFKKYSTALKRLGYTDRALAFWEKHTNLFSLEELAKHYEHAVKDYEKALEYC
                     SRAIVIVNVSPDDNELNSRYKARFAHRINRLQKKLQWKHIAP"
     gene            complement(11031..13310)
                     /locus_tag="PLX22_05110"
     CDS             complement(11031..13310)
                     /locus_tag="PLX22_05110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT19309.1"
                     /translation="MNIQQFIEYLTNYEPFIKQVAAWHTIPAKEAEYALYPDNCDASI
                     IAVYKKRGIEKLYTHQRSACDAVQRKENIVVVTPTASGKTLCYNLPVLNHIVKHPDAR
                     ALYLFPTKALSQDQLSELQEIIGFAGLSVKTYTFDGDTPQSARKAIRTAGNIVITNPD
                     MLHSGILPHHTIWVKLFENLQYIVVDEIHTYKGVFGSHFANLMRRLKRICSFYGSNPQ
                     FICCSATIHNPKELAETIFELPFTIIDNNGAPRGKKHFVIYNPPVVDKALGIRASAIK
                     EAASIGSLCVKNNVSTIIFCRSRLRVEIIATYLKEKCNNYSIAAYRGGYLPNERRQIE
                     KGLREKKINAVVSTNALELGIDIGMLDCAISVGYPGSIASLQQQSGRAGRRGAESLAI
                     MVATSSPLDQYIARHPHFVFENNPETANINPDNLLILMDHIKCAAFEIPFSTDELFTH
                     HHLTTKEMLHYLASKNILKESGGKFHWMSDIYPANDISLRSASQDNVVIVHNDNHAEV
                     IGQIDYPSAPTMIHDDAIYIHQGQQYYIDKLDWERKIAYCHEVESDYYTDAEEKTDIH
                     VLEEFNNKSFSIATLSLGEVNVRTKAIMFKKIKFGSHENIGWGKINLPELEMHSTSVW
                     CSFTDECVQQFEREEAGEVFIGLSYILRTIAPLYTLCDYGDIRVVFENLSHFTQRPTI
                     FIYDAVPGGVGISERIFAIFGLIAQASKEVIAACTCTNGCPACIGPMITRTGIKEKII
                     ALLEILHNEFEGQTGHILH"
     gene            complement(13313..14161)
                     /locus_tag="PLX22_05115"
     CDS             complement(13313..14161)
                     /locus_tag="PLX22_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOT19310.1"
                     /translation="MAMEEQSLELSHKAILQPLLSQIQLPISEYSFANLYLFRKEHNY
                     SYISGDIVAVRGITYDGTSYIMPVKPLDCEYAEKLTQLLLPGSCLFPIADDWLHFFTE
                     KGLSYYYNDGDSDYVYTKEKLATYPGKKLHGKRNLLYQFTAKYSTTVLPLTSERINDA
                     LYVLDEWKKESGTSADVSACSEACMLLDELNLCGSIYYVDDKPAAFIIGEAITVDMFA
                     IHFAKAITSYKGIYQYLYNHCANALPANYKFFNFEQDLGVEALRFAKRSYQPDMIVTK
                     YRVSYP"
BASE COUNT         4246 a         2466 c         3020 g         4514 t
ORIGIN      
        1 ccttaccgac tgtttgattc tgttttcccg gacgggacat tgcaggaagg caggataagc
       61 tatatcaata gtttacaccc ccatatggtt gtatccatac acttttcgtt aaatgcttca
      121 cgctactatc gaggtatgaa tgcagttatt gccgccccct atagttttat gtataagggt
      181 ttacagtatc ttcagggtaa tggtggaaac agatcatttt tctataatag caactatgct
      241 gactggttca ctgagactga agataaatca tcctttttct ggtactgcaa tgatgttatc
      301 cagtatttta tggggtatcg tatgaccaat aactatcaac cagacataga agctttcagg
      361 ggataccgct acaatatggt tcagtggata tataacgatc ctccgggatg ggctcatata
      421 gcaaagtacc atccggcaaa aactgcatac gccgataacg taaagcaatt tataccaggc
      481 aatgcttttt ttaagcgtga acaatctatg tatgagaaat accgaaggga tggcgggtat
      541 gagggatatg gtggtgataa cctttatgca tcaaatgaaa ttatacgata tgtattatac
      601 aacctgtacc tgcaaggtgt gcggcacaaa gagcagcgcc ttgccccgcc gtatatttcc
      661 atctggtcaa tacccctcca tgtcaatgct atcaatgcat acatggaatt aggatattta
      721 gcaaggccct atacacggta tattctggat aaccatctgg atgatatggc tgaaggtata
      781 gcagcaggta tatactcact ttttgctggt attgaggttt caaacaaaat tccatgtgcg
      841 ccatatggaa aaaagattga ccttaaaaaa tatactatca ataaaaacaa taattacttt
      901 accattgttc gatagttact gtaaaggtgg cagatagtga aaaaattgag cattatagca
      961 gcattgattt tttttcagac acttgtttat ggagataata taagtcagcg atttattgct
     1021 gctgcagata aagctggcaa ggctgttgtc agcatccagg tatttgttgt tgaaaaagga
     1081 cactaccaga aggttgggta tgcttcggga accataatat catcaaaggg gcttgtggtt
     1141 accaattacc atgtggttgc aaaaggcaca gtatatcagg ttaatttatg tgatggaact
     1201 gaatgtgatg tggagccatt tgctgatggc agtttattca aagctgatga aaaaaccgat
     1261 atagcccttt tgaaggtaaa acattctgcg ggattgcctt taacacctat tgagtttgag
     1321 gataaaaaag tatttccagg ccaatgggtt atggcaatag gcaatccgtt tggtttgcag
     1381 caatcggtaa ctgctggtgt tgtatcatcg cttggcagga gtgatattgg ttttgctgat
     1441 attgaggatt acatccagac cgatgttcct attaatccgg gcaattcagg aggcccgctc
     1501 atttcacttg acggtaaact tatagggatc aatactgcta tacgtactca aagcggtgga
     1561 tatcagggaa taagttttgc tattcctgct ttcattgtaa aacaggtggt tggtgaactt
     1621 gttcaatatg gcagagtacg gaggggatgg ttaggatttt tggttcagga gaagatgatt
     1681 aataataatt caccggtaca tgtagagatt atttctattg ttgatggttc accagcatat
     1741 aaggcaggta ttgaggttgg cgatattgtt agagaggtgg atggtatccc cataaaaaag
     1801 atttctgatt taatgaaggc aatacattcc aaacaaattg gttctctggt taacatagtt
     1861 attgctcgtg atggacagtt attaaatttt gattttgttt tacaggaaaa aagtaaaata
     1921 tccagagtta attattggat taatgtatta cagcaatcat atggaattac tattgaagat
     1981 acgctgtatg gtgctgtaat taccggtgta aatcaatttg gtattgccta taatgtacta
     2041 aaaaagggtg acattataca atcaattaat gataaacagg ttacatcatt agatgatatt
     2101 attgcaatag tcaaaaaaac aaagggtaca attgaaaatt gtgttgtgct gcgctcaggt
     2161 aaggtgatac gatgttactt taatctggat ggaactgaat aatatatgaa taaacggatt
     2221 atacttttta ttattgcagc tttcatgtct atgtgtattc aatcattttc tgatgaaaag
     2281 aaagctgaag ttatactgga caatgaattt tatacatcgt tgcaaaatag cgatgctatc
     2341 aagcgtataa gaacatttga tatgcttaaa gatgtaatag taaaaggaac aggaaatgta
     2401 gtaaaagttg aagttgctga tgttttgaaa aaaaaattca gaattgttct gtcgctcaat
     2461 ctgcatggca tacaaattct ttattattgt tatactgatc aggatgagta ttttgactta
     2521 ttacaaccgg atgacctatt tgaatttagc ggccaatttg ttgttgcaac tccgttgaat
     2581 gtaaggtttg atacctttat tattgatgta ttgcttgaag aaggtgcggt tgtagtgcat
     2641 taatattatt cgcgataaac agtgaagacg tagtctaaaa tggatatcct atctgcatca
     2701 tctaacccaa caaaggtaat gtcgtacaaa gtatcatccg aagctgttac tttaccggta
     2761 atatcaattt ttttagttcc taaagtaaga tgccctttaa cataatgttc taatggtgta
     2821 gtggtttgca atagggcttc tctatctgtt aattctttga cgataccttc atgccattgt
     2881 atctcttcag atgaaaaact tctggacgta ttgatggaaa aagtaaaaaa agtgaatgga
     2941 atattgacct gtgccttcaa acatcgtcgt gagggattat atactattgt atcggtatgt
     3001 tgcagaagaa taattggaaa tggagcatta atttttccca gaatagtagt attaaaatca
     3061 tacgagccat caggataagg ccataggtag cattctacag tatcattttc tttcacatgt
     3121 gataaaaaat cttctgaaga tttacatagt aaaaaaagtt cattattctc aatcaccgtt
     3181 aagagagcat atccataacc ttttttatac actctgacgg gtgtatttgt tttaatatcg
     3241 gctgtttttg caatgttttt catgggatac ccctgaatat aaatatgtag tagcaattaa
     3301 taatgtaaat tgtatggtta ttgttacaat tactttctcc atttactcta tcactggaga
     3361 aacagattta aaatttaatt ttagtagcca ccttttatag aagcatggct gtgcagtcaa
     3421 gggtaaattt gttactatgc aataaaatag atatttttta aaaaattgaa aacctgacaa
     3481 aaaagaattg aaaaaaaatt gttgcaaatc atactatgtg caaaaatagt ggtattcctt
     3541 ctgctacata cactatgtaa tgtagctatt aataaccata gggggtaaca gtgaataaat
     3601 acgaattaat agtgatttta cgtcctactg ataagctgga tatactcaaa gaaaagcttc
     3661 gagatatctt aaaaaagaat caggtggtca ttgaatctga tgaaccatgg ggcgttcgtg
     3721 ctatggcata tgctatagac agagcaaatg acgggtatta tgagtacttt attgtacaga
     3781 tagatccagc tcagatcagt gctattattg ctgaattcag attacaaccc gatatcctgc
     3841 gttatctatt tgtacgattg ccaaatttaa gcaaaacagc gtaaattacc atgccaggcg
     3901 atctgaataa agttatactt gttggcagga ttgttaggga ccctgagttg cggtatactc
     3961 cagggggcgc cgctgttgtt tctttttcaa tagctaataa ccgcatatat gtgaaaaata
     4021 atgaaaagca ggaagttgta tcatttttca attgtgttgc gtggggtaaa ttagccgaaa
     4081 cagttgctaa ctattgcaaa aagggacagc aattagcctt agaggggaga ttacaacagc
     4141 gttcatggga cgataaagat gggaacaagc gttccaccgt tgaggtggta gcagaatcat
     4201 tgcaattttt aggtggaaaa ccgcatgctg aaacatcacc atcagtagac agcatggaaa
     4261 ctggcgatat gggttctacc gaaccattct ctgatgatga tattccattt tagtaaaatt
     4321 ataattttat tttttaaaga ggtatataaa tgagtgaaga aataaatcag ggtgaaacta
     4381 tacaggcaca ggaaaatata attccggaag atacaaaatc atctgaagtt gcaagacatt
     4441 cagaggataa agcattatct gcagccgtgc aggtgcagcg tgtaccaaaa tttaaaaaga
     4501 aggtgtgtcg tttttgcgag gatcctaatg tagctattga ttataaaaat ccggatttac
     4561 tggtcaggtt tattactgac cgtggcaaaa tattacccag acgtattact ggcacctgtg
     4621 caaaacatca gcgccattta gcacgtgcaa ttaagcgtgc aaggaccata gcactgttac
     4681 catttgtggt aaaataggtt gtatttgcta ttgtggaact tatagtacat cttatagctg
     4741 gtatagcgtt atgtgttatt actgctatag tagtttataa attgaaatat aaagctctgg
     4801 cgatagctct ggtcatggtt ctatcggctt tatttttagg tctatcatat ttttataatg
     4861 agatgattgg tcttgagctt attataacgg gtgctgtgtt tggatatgtg gcaaaacggt
     4921 tttcattcca gcgatatttt attaccagcg ttaccatagt atggcttttg cttttgatta
     4981 ttcattatat actctatttt gtaaaaggaa ttgatgctat atccggcaat caaaaaatta
     5041 ttgaggaaat cattaatgct tcatcaattc ctgttactga aaagcaggtc ctgtcacaac
     5101 aattggaaga ctttttacca ctgatgcgcg tgcttatgcc gttttcatat tttttcagtt
     5161 ctgctgcaat ttcaatttta gtctatgtat ttgtttttgc cttctttgta cggtttaagt
     5221 ttttacagac tgaagacaat acaatttcgc ttttccgtgt tgatgaatgg gtggtttttt
     5281 tgttgatagc aagctggctt gttgttctat taggtaaaga attacaaact gtttgggcga
     5341 tagctctgaa tattggactt gttttatgtg tagtctatac tatacagggt atatcggtta
     5401 tactgttctt gtttgaaaag aagcaatggc ctaaattgta tggattcctt atactttttg
     5461 ttacaatttt attaggattc caggtgatgt tttttgttat caccatgctg atgggtatag
     5521 gtattattga tttctggatg aatttcagaa agattgcata aattcaattt tcggaggaat
     5581 ttcactatga gagtaatatt acagaaagat gttcaaaatt taggtgaagc tggtgatatc
     5641 aaggaagtag cagaaggatt tgcccgcaat ttccttttgc ccaagaaact tgttatacca
     5701 tataatgagt caagtgcaaa agcaattgag catcaaaaaa aactagttaa aataaaaaag
     5761 gaaaaacgta aaaaagaagg gcagaagctt gcggaggcat tatcaagtgt tgccttaact
     5821 attggtgctc aggttggtga ggatgataag ctatttggtt cggttactgc tatcgacatt
     5881 gccgacaaac ttttacaaat gggatatacc attgataaac gaaagatagt gttgcccgaa
     5941 cccataaaga aagctggtga gtatgaagta aagattaaat tagaagaagg attaacagct
     6001 actataaagg ttagcgtggt aaaggagtaa tcgcatgctt gatgttggca tgcctcctca
     6061 tgatacagag gctgaagctg cgtgtttatc ttctatacta ttaagtaaag atgcattgct
     6121 aaaggttata gaaatcctcc aggtggagga tttctatatt gatgcacata gaatgatcta
     6181 tgaggcaatc agggagctgg aaataaaatc agttcctatt gacctcatta ctataaaaca
     6241 gcggctcatt gataaaaaac agtttgaaaa agttggcgaa gatcaggctc tggtcaatat
     6301 ataccgtact gtatctacct cagctaatgc tgaatattat gcaaacaggg tcaaagagtt
     6361 aagcttacgc cgtaaactta ttgaaacatg caccaccatt gccgaacgtt catacgatac
     6421 aacattggat acaaaagttt tactggatga tgccgaaaaa gatattttcc tcattacaca
     6481 gcgcagaatt accagcgatt ttataagttt agcaaatgtt ttaaaagaaa cccttgaaaa
     6541 tatagggcac atgtatgaga caaaaaggcc ggtcacaggt attgccggtg gatttactga
     6601 ccttgatgca atgcttaccg gctttcatcc ttcagagctc atcattattg ctgcacgtcc
     6661 ctcaatgggt aaaactgcgc ttgcgctgaa tattgccaat catgttgcat tgcgtgaaaa
     6721 gcaaccaaca ttgttctttt cattggaaat gcctgctaca cagctagcta cccgtatctt
     6781 atgtattgaa tcattaattg atatgcagca tgtccgaacc ggtcagataa gcaatgagca
     6841 gttgcgcaaa ctggttgata tttcgggcag acttgagaaa tcaccaatgt ttattgatga
     6901 cacccctgga ttaagcatat ttgagttgcg ggcaaaagca cgacgatttg cccagaagca
     6961 gaaggttggc ttaattgttg tagactatat gcagcttata gttgcaaata gccgtttaga
     7021 gcgtcatcag caaatagctg aaataagcaa tgcattgaaa caattagccc gtgagttatc
     7081 ggtaccggtc attgcactat cacagctttc acgtgcggta gaatcacgta ccgaccagat
     7141 gccacagttg tccgacctgc gcgagtcagg agctattgaa caggatgctg atgttgtgtt
     7201 atttatttac cgtgaagaga aggtaaaaaa agaaaccgag cgccttggca ttgcagacat
     7261 cattgttgcc aaacagcgta atggcccaac agggaggata gagctgcgat tctgggataa
     7321 atacatgaag tttggaactg ttgaaaaatt cagagaagaa gctgaatgat atgaataatg
     7381 ctgagattat attcctccgc cctgttatga aattttttga gtctgtaggc aaccataccc
     7441 ttttttttgc tcaaacggtt aaatggattt ttaaaccgcc gtttgataca aagaatattt
     7501 tattgcagat gcttgaaatt ggctataaat cactgccggt aacatgcata acgctttttt
     7561 ttacggggat ggtaatggca ttgcagattg gcagggcaat ggattcgctc atgtccggtt
     7621 catcaatatt tatcgggagt gcggtgtcca ttgccatgtt cagagaactg tgcccggtac
     7681 ttacagcgct cctattatca ggaagggtag gttcagctat tgctgccgaa attggcacca
     7741 tgcgtgttac cgaacagatt gatgctctca ttacgcttga tgccaatccc attcagtacc
     7801 tttcggtgcc acgctttatg gcatgccttg tcatgacacc ggtactggtt gtcatcaccg
     7861 atgttgtggg ctcgttaggg ggagcattta ttgcatattt taatttagga attactatcg
     7921 ataaatatat tgagaatgtt tttaattatt tgcaggttat ggatttttta acaggattat
     7981 tgaaatcagt tttttttggt attgagattg ccatcatagc ctgttatcag ggtttcaata
     8041 catttggtgg agcagaaggc gtcggcaaat ctactatccg cgcagtggtt gcatcatcaa
     8101 tgaccatact catatccgat tactttctta catacctttt acaatttatt gaatagaatt
     8161 aaagaattgc ctatgaaatc gctgaagaaa agaaaagaag agtttattaa gctattgcaa
     8221 aaaaacagga taccaaaaag gattgttgat gcttttgcga tagttaatca ggaagatttt
     8281 tttgatgaaa tatatagcga tgcactgtat ggaacacagc ccattcctgt agcccctggt
     8341 aaaacaggtg atgagccgct gacattagca cggatgattc attactgtgg cattaaaaag
     8401 gatttccatg tccttgagat tggcaccggt tcaggatatt caacagcaat tatggcacac
     8461 cttgcacaat ctgtaacaac tgttgaaatt gatgaagagc tggcattgct tgcaaagaag
     8521 cgattttccc gcttgaagtt aaccaatatc agagtttttg caggcgatgg taccactatt
     8581 gatgaatcat cagggatttt taatgcagcc gtgatatttg caggttgcac aaaaaggcca
     8641 ttgatgctgc ttcatgtcat gaagcaggat ggcgtagtgg tgtttcctat gggtccagct
     8701 catcaacagc agattgcact gatgcttaat cacagggatg aaaaggttga aggcgttttt
     8761 cctgttgtgt ttaaggagtt ttgcaccttt atgccgattg ctggcatata tggatgatta
     8821 tatatctgat tttttaattt tatccggatc ctcatcgggc ttgtaacagt aacggcaacc
     8881 atagattaaa atctttgtgc tgtcttttac aatttttgat gcatacagtg cctcatagcg
     8941 cgaaagcttt gagccacata cagggcatgt tctttctttc atggaagcag gggtgaaatt
     9001 tttatcaacg gttttagctg gcattacagt ggcagcttta tttgtctctt tttttgcaga
     9061 ttgcgaagtt tttttttgca gatgatgata cagcgaaagc atatataaaa ataatgcaat
     9121 acctgctcca aacgacagta tatataaaat aatctcaacc atggatgcta tgcccttttt
     9181 gttataacgt taatcataat ggtatgatcc gttactggtt tcatggtata atctcctgct
     9241 atatccacta ccaataatcc acattttttt ataataggaa ttatctcatc aatggtgtat
     9301 atgcgttgca gatgttcttc aataatggtg cggctttgtg ttgcaaatgt caatttagta
     9361 tataccatct ttgatttttc atcatattca ttataccatg ttatatccgt tccttttact
     9421 gtatatgcaa aaagcctgcg atgaaagttt ttcattatat tatactgcgt ggtaatatca
     9481 aagaggaata tactgttata cgacatgcaa ttgtatgcac aacgcagtgc ttttgcaaaa
     9541 tcatcatagg taagcagata gttaaaggta tcgtgtactg aaaatataaa atcaactgtc
     9601 tttgatagtg caaagcaggt aatatcacca caaaagatgt gaaaattttt attagcccgc
     9661 gtttttgcaa tggataacat tgctattgat ttatccatgc cgtatatttc ataatcatcc
     9721 atggaaaatt ttggcccaaa cttgccggtg ccgcagccca gttcaagcac cgtgcgtgga
     9781 ttggaaatgt acttgcacat aacgttgcgc aggtatcggt accattcatt atagttaaca
     9841 tgctgaagca tgtagtcata atacggtgct aagtggtgat attcgttcat ggggcaatat
     9901 gcttccattg taattttttt tgtaagcggt ttatccggtg agcaaagcgt gctttgtagc
     9961 ggctgttgag ttcattgtca tctggtgaaa cattaacaat aactatcgcc ctgctacagt
    10021 attcaagtgc cttttcatag tcttttactg catgctcgta gtgtttggca agttcttcaa
    10081 gtgaaaaaag gttggtatgc ttttcccaga acgccagtgc cctgtcagta taacccaatc
    10141 gctttaatgc tgttgaatat tttttaaaaa gcgaatgctc gttatggagt aatgcaaatc
    10201 ctgcatattc aacaagctca acaaacaact gaatgttacg gctataaaga tgtttggcaa
    10261 tgtttgtata aaggtttgat ggtacctgat gaaattgttt tgcatgcagc atttgataaa
    10321 gggtgttaaa aaaaacaagc agtaaaacca ttgaggttat gtctatacgg ttgtgttcaa
    10381 taacattgat aatacgcggg tcaaaacctg tttgctgata ggtaaaatat acgtcgggga
    10441 taaggtagcc agggatatcg tcggtacggg taacatcaag caaaagttct tccagcgatt
    10501 ttagcgagca gcttgcataa atagatttga atattgagcg ggctggtttt agcagatcaa
    10561 tgacaggcat tgatacggga aagcccggta tgcggttaat cctgaacctg gtttttataa
    10621 tgggaatatc aaatgttttc ccgttaaagg ttacaatagc tttgtagttg tttaccctgt
    10681 cagcaaaata gtagagcagt gctggttcat cggcgtattc acgcatgaat agttgctcaa
    10741 cggtaaagac gttattggca aagtatccca tgccaagtaa aaatgcgtag tttcctgttc
    10801 cgcttgtaag ggatgttgtc tcaatgtcaa aaaatataca ctcatggaga tgcaggggtt
    10861 ctatatggtg tatgcgacag taatcatcaa gataaagctg ggcggtagtg atagggaagc
    10921 tatcactatc atggtggtga gcaaatagcg tgacggctcc atggggagtt gttatggtgc
    10981 tgccgccatt gagtgatatc catgaactat cgtgcacaac agtttcctgc ttagtgtaat
    11041 atatgtccag tctgtccttc aaattcattg tgcaatattt caagaagtgc tattattttt
    11101 tcttttatcc ctgttcgggt aatcattggc cctatgcagg caggacagcc gtttgtgcag
    11161 gtgcaggcgg ctataacttc ttttgaggct tgagctatca aaccaaaaat agcaaaaatt
    11221 cgctcggaga taccaacgcc acctggaacg gcatcataga taaatatcgt tggcctctgg
    11281 gtaaagtgtg aaaggttttc aaacaccacg cgtatatcgc catagtcgca taatgtgtac
    11341 aacggtgcaa tggttcgcaa aatataggat agccctatga aaacctcccc ggcctcttcg
    11401 cgttcaaatt gttgcacaca ctcgtcggta aagctgcacc acactgatgt ggaatgcatc
    11461 tccaattcgg gaagattaat tttgccccag ccgatattct catgcgaacc aaattttatc
    11521 tttttaaaca tgatggcttt ggtgcgtaca ttgacctcgc ctaaactgag tgttgctatt
    11581 gaaaatgatt tattattaaa ttcctcaagt acatggatat cagttttttc ctctgcatcg
    11641 gtataataat cagattcaac ctcatggcag taggcaattt tacgttccca gtctaatttg
    11701 tcaatgtagt actgctgtcc ctgatgaata tagattgcat catcgtgaat catggtgggt
    11761 gctgatggat agtcaatctg accaatgacc tcagcgtgat tatcgttatg cactatcacc
    11821 acattgtcct gtgaagcact gcgaagcgaa atgtcatttg caggatagat atcgctcatc
    11881 cagtgaaatt ttccgccaga ttcttttaag atattttttg atgccaggta gtgcagcatc
    11941 tcttttgtgg ttagatggtg atgggtaaac agttcatcag ttgaaaaggg aatttcaaat
    12001 gctgcgcatt taatgtgatc cataagtatc agcagattgt cagggttgat attggcagtt
    12061 tccggattgt tttcaaaaac aaaatgagga tgtctggcaa tgtactgatc aagcggagag
    12121 cttgttgcaa ccataatggc cagtgattcg gcgccgcgac ggcctgcacg tcccgattgt
    12181 tgctgaagcg atgcaataga gcctggatag cctactgata tggcacagtc gagcatacca
    12241 atatcaatgc ccagttctaa agcgttggtt gaaactacag cgttaatctt tttttcacga
    12301 agtcccttct caatctgacg ccgttcattg gggagatatc caccacgata tgcagcaata
    12361 gaatagttgt tgcacttttc ctttaaatag gtagctatga tctccacgcg cagcctgctt
    12421 ctgcaaaaaa tgatagttga aacattattt tttacgcaca gcgagcctat gcttgctgcc
    12481 tcttttatgg ctgatgctcg tatacccagc gctttatcca caacaggagg gttataaata
    12541 acaaaatgtt ttttcccgcg cggtgcgccg ttgttgtcaa tgatggtaaa gggcaattca
    12601 aaaattgttt cagctaactc ttttggatta tggatggtag ctgagcagca gataaattgc
    12661 ggattgctgc cgtaaaacga gcatatacgt tttaaccgtc gcatgaggtt ggcaaaatgg
    12721 ctcccaaaaa cgcctttata ggtgtgtatt tcatcaacaa cgatgtattg aagattttca
    12781 aaaagcttaa cccatatggt atggtgaggc aaaattcccg aatgaagcat atctggattg
    12841 gtaatgacaa tattgcctgc tgttcgtatt gctttgcgtg cagattgtgg tgtgtcgcca
    12901 tcaaaggtat atgttttaac cgatagtccc gcaaagccaa tgatctcctg cagctcagaa
    12961 agctggtcct gtgaaagcgc ttttgttggg aaaagataca atgctcgtgc atcaggatgt
    13021 tttactatgt gattgagtac gggaagatta tagcacaatg ttttaccaga tgctgttggt
    13081 gttacaacaa caatgttttc ttttcgttgt actgcatcgc aggcgctacg ctgatgggtg
    13141 taaagctttt caatcccgcg ttttttatac actgctataa ttgatgcatc acaattatca
    13201 ggatataagg catattctgc ttctttagca gggatggtat gccatgcagc aacctgtttt
    13261 ataaatggtt cataatttgt aagatattct ataaactgtt gaatattcat tgttatggat
    13321 aactcacacg atattttgtt actatcatat ccggctggta tgaacgcttt gcaaagcgca
    13381 gagcttcaac acctaaatcc tgttcaaagt taaaaaattt atagttagct ggtaatgcat
    13441 ttgcacagtg gttgtaaaga tactggtata ttcccttata gcttgttata gcttttgcaa
    13501 aatgtattgc aaacatatca acggtaattg cttcgccaat aataaatgct gccggtttat
    13561 cgtcaacata atatatactg ccgcacagat taagttcgtc aagcaacatg catgcttcac
    13621 tacaggcgct aacatcagcc gaggtgcctg attctttttt ccattcatca agtacataca
    13681 gcgcatcgtt tatccgttcg gatgtcagtg gcaaaacagt ggtagaatat tttgctgtaa
    13741 attgatacag caggtttctt ttgccatgca gctttttacc ggggtatgta gccaattttt
    13801 cctttgtgta aacatagtcc gaatcgccat cgttgtagta gtagctcaac cctttttccg
    13861 taaaaaaatg aagccaatca tcagcaattg ggaaaagaca gcttccggga agtaacagtt
    13921 gcgttagctt ttcggcatac tcacaatcca ggggtttaac tggcatgatg tagcttgtac
    13981 catcataggt tatgccccgg accgcaacaa tatctccgct tatatacgaa tagttgtgtt
    14041 cttttctaaa cagatataaa tttgcaaacg aatattcaga aattggcagt tgaatctggc
    14101 ttaaaagcgg ttgtaaaata gctttgtgcg atagttccag tgattgttct tccattgcca
    14161 taactaatcc cttttatcaa tatgcctttg tccgggcata gtacggagta ttccttcttt
    14221 ttcaatctta tttttttatt ttataa
//
LOCUS       DAOSOX010000047        13886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4437_length_13886_cov_8.244306, whole genome shotgun sequence.
ACCESSION   DAOSOX010000047 DAOSOX010000000
VERSION     DAOSOX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4437_length_13886_cov_8.244306"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLX22_05120"
     CDS             <1..347
                     /locus_tag="PLX22_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19311.1"
                     /translation="DNYLKKAVTFYKKKNYDNALAILNSISTMSIPASYNYLKAKLLF
                     KNRHYEDAKQAILLALKEKPDHKEFNYLYGQICFALKDFNTALDIFADISMMQPYKKS
                     DEFIELIRKKIE"
     gene            complement(387..1634)
                     /locus_tag="PLX22_05125"
     CDS             complement(387..1634)
                     /locus_tag="PLX22_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOT19312.1"
                     /translation="MEREKVLELKQVIGKKLNRPMLYYLDLCTRCGLCYDTCHVYQGI
                     PKKEYSPVGRAEVVRRVFRKYFKPAGKFFPWWGDAHEFDENLMEELREAAFSCTGCRR
                     CTMFCPFGIDMPYILGIAKELLIAYGNAPEELVMLADGAVEKGKSIDMFKEGFRGVIK
                     DLEQEVQKLIGASSSDGLIPMEKHGANILFVGLSGAHSIIYPAAIFNAAKEDWTLSYF
                     EAVNFGYFAGDTEKSNFIANRIINEAKALGVKEIVIVECGTAYRIPKFLMGPLPFKVS
                     SIVEVIYRYIKEGRIKIKQGIVSEPVTWHDPCQLGRNGGIYEQPREILQIMANDFREM
                     NPNCEYNWCCGGGGGLVALDNEEFRIKSGKPKKEQIEKSGASMVVTACENCHLQLSTI
                     SQGYNMGVKVESLTKLVGENLII"
     gene            complement(1637..2359)
                     /locus_tag="PLX22_05130"
     CDS             complement(1637..2359)
                     /locus_tag="PLX22_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOT19313.1"
                     /translation="MSGYLSYFILIPMVYIAFATLIFGLIYRFYSIWKAPAPVGTGAI
                     FPKKGSKVSGLIYDAMLVPMAYAKQKGFWFIIMLFHIAFFFLFLGHLELVADFKFIQI
                     IPHRIFLGGGLVGIILMLTTLYFLFRRFGTPYREISIPEDFIILLVLFFCILFGSILH
                     LAERYSDWGAVLRVDINVYRNWLQSLLLFKPQLSYNITELSHYTILVLHVLFANIFLM
                     MFPFSKMIHSVFTFLAHYRKRN"
     gene            complement(2384..2623)
                     /locus_tag="PLX22_05135"
     CDS             complement(2384..2623)
                     /locus_tag="PLX22_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOT19314.1"
                     /translation="MSDIKADVTMDLKGLACPLPVVKVSQQIKKMQVGQVLMAETTDP
                     GALADFPAWAKTSGNEIIDTVKDGTTTKFFIKKLK"
     assembly_gap    2777..2876
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2877..3049)
                     /locus_tag="PLX22_05140"
     CDS             complement(<2877..3049)
                     /locus_tag="PLX22_05140"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT19315.1"
                     /translation="MDVNKTLESIQNQIKEIQDGRKDKIAMIVFSGDLDKLLASFIIA
                     TGSVAMGMEAVMF"
     gene            3371..3559
                     /locus_tag="PLX22_05145"
     CDS             3371..3559
                     /locus_tag="PLX22_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19316.1"
                     /translation="MNNKPVTNNEAHNDDNRLHFASTNEALYALNMYMNRFMWISTRE
                     IVSGISSWYYNDTKGEKL"
     gene            complement(3621..3836)
                     /locus_tag="PLX22_05150"
     CDS             complement(3621..3836)
                     /locus_tag="PLX22_05150"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOT19317.1"
                     /translation="MKKNVGTADRYIRVLIGVSLLINIFIVKPGAIGTIILLALGLAV
                     LYSAYSGYCWIYDLLKISTCKKSNDAE"
     gene            complement(4075..5010)
                     /locus_tag="PLX22_05155"
     CDS             complement(4075..5010)
                     /locus_tag="PLX22_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT19318.1"
                     /translation="MKCTIVTVYITTMKRYCIVIIIAIALVFNTIACYKQHIPSDGVY
                     TVWVHSDIQPTKKSERWHYARAIDDIMNIKPHPIFAIIAGDIVQHKEAEEDYQWFIEL
                     RKKAEINYWYEIAGNHEWKNMPAYNTYIGKPLRYTVLFGNIIFIFMSNERGRPPTYIS
                     DETFLWWKNIVITNQDKNIITVTHAPLKNSNLIGTVSKKHIIINSERFEKVLQQYHVD
                     IWISAHIHLPAWIGQNSKIIPEFNNTLFINVYGIRKDPTTNVESRIFFFTHGSKKVLI
                     RLRDHEAETYKSTYDINHKLHYPFTCNNCLPEMIQ"
     gene            5091..6359
                     /locus_tag="PLX22_05160"
     CDS             5091..6359
                     /locus_tag="PLX22_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT19319.1"
                     /translation="MDNNITIDTSRFQEISVSYLKKLSGDKLLTFDVLDQQGNTVFSA
                     GQALDDELLKNKSEEGIKFFIPRVTESIGVYDREIIDMKKLKAIADDTATMYTDIRKN
                     GKMTHAQFLQSHNHLAAIVDSLRPEDKTGGVLSLLKDIKDFDYTTYTHSVNVGLLAMI
                     LVYKEMSTGDKVKLAALAGYLHDIGKLRVEQSIIQKPGLLSQDEFKKVLEHTREGYRI
                     LDSVVDSTGKKVVPNSIKYVALFHHRKLQSPGYPFRETDKDKTLENQYSELPDLARMI
                     GICDMYDALTSATPFRIPMGVEKALRYILSLSNYLYSTDDIHRFIKVLGLSLNKGTPF
                     LKAGDFIMLESEKISMDTRKKVRVYEVARIEEISRINFMNPKVMIFYDTIKNKKVHNI
                     IVDLRYDSNRRVVRVFESTRIRNMLLRQLK"
     gene            6370..6738
                     /locus_tag="PLX22_05165"
     CDS             6370..6738
                     /locus_tag="PLX22_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT19320.1"
                     /translation="MNPILLIFDNDRFAEFIRFVLTTAGLKIELLADPLEIFEKVKEM
                     NPPLIVMDIFLKNIDGLYLLERLYSVKEFTKIPVFIVSSRNEPLAVFDALERGAADYM
                     AAPINEDMLIEKVNKLLKTN"
     gene            6883..7908
                     /locus_tag="PLX22_05170"
     CDS             6883..7908
                     /locus_tag="PLX22_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT19321.1"
                     /translation="MKKCMLLVLIAPIFLLQCSSQINLYDTSSNGQSNNTINAYTMAE
                     SLSFKEVWAYLMKGEEDKLTGEEKITDLCYFSAKVNGKGQITDDYAPPAIKAENRIRH
                     HIVIAILDNYALTHFIIHPDFKVRKGFIKDICRISQKFDGVQIDFESVASSDAHYFFS
                     FLSELRANLGKSKVVSIALPARRSRIADAYDYPSIAALVDRIIIMAYDQHWYGSNPGP
                     IAGLSWCREVGEFAKSFIPQQKLIMGLPLYGRCWQDKNFNKSMHYTHIEQVMQTSNIQ
                     PRYDPENGSYFEYEEKIKVRVYFNDERALYERLKLYSSMSIDNVAFWRIGQGPKGMWQ
                     GISTKSL"
     gene            7934..8467
                     /locus_tag="PLX22_05175"
     CDS             7934..8467
                     /locus_tag="PLX22_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOT19322.1"
                     /translation="MKKLSYDDIQSNRPDKNELFRLQRFPVVTILENIRSLYNVGSMF
                     RTADAARIAYMYLCGYTPHPPRKEIEKTALGSTESVPWEHSTKPIQIIRMLKNQGFVI
                     AALEHTTTGVAYTTYNYSYPLCIVVGNEVHGVSDDVLAMCDAAIEIPMFGIKHSLNVA
                     VAYGIVVYHAVMTYQMK"
     gene            8486..>8923
                     /locus_tag="PLX22_05180"
     CDS             8486..>8923
                     /locus_tag="PLX22_05180"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19323.1"
                     /translation="MQKVILKQGKEKSVLQRHPWVFSGAIATVDADDGAMVPLCSYDG
                     TVIAWGYYNSRTQIAMRLLTFSDTKPDDDLIARRLMNAYALRVESGVVKSTNAFRMVH
                     SEGDMLPGLIADWYNGHCVVQFLTSGMDRLKGTIVQIIQEKLQP"
     assembly_gap    8924..9023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9024..9455
                     /locus_tag="PLX22_05185"
     CDS             <9024..9455
                     /locus_tag="PLX22_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOT19324.1"
                     /translation="DSSGYALSIAQKNCELNNVGKRHAGVKADVFDYINTNDIPQNVI
                     ICDPPALIKSKSAIQQGLRGYKELNVKIFKKVPPLSFVLTCSCSRFVDMKLFQKIVFS
                     AARDAGREVTITGKYLQPADHPVSIYCPETEYLKAIMVFIS"
     gene            9625..10464
                     /gene="nifU"
                     /locus_tag="PLX22_05190"
     CDS             9625..10464
                     /gene="nifU"
                     /locus_tag="PLX22_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOT19325.1"
                     /translation="MWEYTDKVKELYTNPKNVGEIPDADVVVEVGSIVCGDALTLYLK
                     LDGDVIVDAKFKTFGCGSAIASSSALTEMIKGKTIAEAARITNKDIVDFLGGLPDQKM
                     HCSVMGQEALEKAIKKIKGETIEDEQEDEGAVICQCFGVTENVIRRVIRENNLATVED
                     VTNYTKAGGACGSCIPKIEDILKEELASAVAQPAATAMKPMSNIKRMQLVEETIHNVI
                     RPVLMRDGGDIDLVDIEGKKVYVALRRHCAHCVVSDVTMKNLVEDTLKEFVENDIEVI
                     EVK"
     gene            10464..11672
                     /gene="nifS"
                     /locus_tag="PLX22_05195"
     CDS             10464..11672
                     /gene="nifS"
                     /locus_tag="PLX22_05195"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOT19326.1"
                     /translation="MNTIIYLDNNATTMVAPEVLEAMLPFFTRRYGNPSSMHDFGGDV
                     EHDMEKARNQVARMLGAEYDYEIVFTSGATESDNMAILGTLQYYRDKKHIITTKVEHP
                     AVLNLCRQLEREGYSVTYVPVDREGNLDVDFLFDSVTDDTAIVSVMYANNETGVIFPV
                     ETIGAFCKQRGITFHIDAVQAIGKIPVDVNALQCDLLAISGHKFHAPKGIGVLYVRRG
                     TRLRPLLYGGHQEKARRPGTHNVPGIVGIGKAAELVMQHLSNSEEVANVKRLRDRLEN
                     GLLSQLSNAHLNGNKNNRVDNTTNIGFEFIEGEAILLYLNEKGIAASSGSACSSGSLE
                     PSHVLRAMGVTFTSAHGSIRFSLSRYTTEDEIQYTLQVMPEVVNRLLAISPYWDAMNK
                     KGKSMTELAH"
     gene            complement(11699..12535)
                     /gene="flgF"
                     /locus_tag="PLX22_05200"
     CDS             complement(11699..12535)
                     /gene="flgF"
                     /locus_tag="PLX22_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HOT19327.1"
                     /translation="MVRGLYTGASGMIAQEARLDAIANNLANVDKAAYKKDLTLFKAF
                     PDMIIRRLNDDGLGITPAGSYDTMPFVGKLGTGVEVNEVYTIHEQGSLMRTENPFDMA
                     MEGNGFFTVMTERGERYTRNGAFTLNKDGILVTHSGLPVLGQNGIIKLQKNNFMINER
                     GEILVNSALSLNPDDMIGLTQNNWEQPVVIDKLKIVDFENIRELKKEGESLYHETQYS
                     GPALPAEEYKVIQGFLEKSNVNAVREMVDMIEVQRSYEANQKAMLTHDQELGRLINEV
                     AR"
     gene            complement(12535..13803)
                     /gene="murA"
                     /locus_tag="PLX22_05205"
     CDS             complement(12535..13803)
                     /gene="murA"
                     /locus_tag="PLX22_05205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT19328.1"
                     /translation="MDFYIVKGGNKLNGTVTISGAKNAALPIIVASLLAEGTTVLHNV
                     PNLKDIQTILQVIECLGAQYQFDTNTNTLTIHVNSLKNAEVPYDLVKTMRASIYVMGP
                     LLARCGEADVSLPGGCAIGERPVDIHLSGFEALGTTITIEHGYIKARVEKLNGNRFIM
                     RKVSVGATANIMMAAVLADGQTILENCAMEPDVVDLGNFLITMGAKIEGLGTERIIIN
                     GVKKLKPVSYSIIPDRIEAGTFLIAGAITRGNVTVNNVIPEHVKACIDTCADMGFVIS
                     GNDSSVTIEPSKKLQGSMVRTLPYPGFPTDLQAPIMSLMCTIPGISVIIETIFENRYT
                     HVGELRRMGADIEIEGNTAIIKGGKQLQAAPVLMSDLRAGASLVLAALAARGKTEIRR
                     IYHTDRGYENFEQKLQALGADIVRVKGGKP"
BASE COUNT         4285 a         2485 c         2705 g         4211 t
ORIGIN      
        1 ttgacaatta tttaaaaaaa gcagttacat tctataaaaa gaaaaattat gacaatgctc
       61 ttgccatttt aaattcaatt tctacaatgt ccatacctgc atcatataac tatctcaaag
      121 caaagttatt atttaaaaat aggcattatg aagatgctaa acaggctatt ctgttagcgt
      181 taaaagaaaa accagatcat aaagaattta attatcttta tgggcagata tgttttgctt
      241 taaaagattt taatacagcg ctggatatat ttgctgacat atcaatgatg cagccatata
      301 aaaaaagtga tgaatttata gaattaatta gaaaaaaaat agaatgatat ataaggggca
      361 aatatctttg ccccttcatg gttacgttat ataattaagt tctcgcctac aagttttgtt
      421 aaggattcaa cctttactcc catattatat ccctgactta tagtagacag ttgtaaatga
      481 caattttcac aggcagttac aaccattgaa gcaccgctct tctcaatttg ctctttttta
      541 ggtttgccac ttttaatcct gaattcttca ttatcaagcg caactaaacc accgccgcca
      601 ccgcagcacc agttatattc gcagtttgga ttcatttcac gaaaatcgtt tgccattatt
      661 tgcaatatct cacggggttg ttcgtaaatg cctccattcc ttcctaactg acagggatca
      721 tgccatgtta ccggttctga tacaatgccc tgtttaattt tgattctgcc ttctttgata
      781 tagcggtaaa ttacctcaac gatgctgctt actttaaaag gcaatggccc cattaaaaat
      841 tttggtatcc tgtatgctgt gccgcattca acaataacta tctctttgac tcccagtgct
      901 tttgcctcat tgatgattct attggcaata aagtttgact tttctgtatc acctgcaaaa
      961 tagccaaagt ttaccgcttc aaaatagctc agcgtccagt cctctttagc ggcattaaag
     1021 attgcagcag gataaattat tgaatgcgcc cctgataagc caacaaaaag tatattggct
     1081 ccgtgttttt ccatgggaat taatccatca cttgatgatg caccaataag cttttgaact
     1141 tcctgttcta aatcctttat taccccacgg aagccttctt taaacatatc gatgcttttg
     1201 cctttttcaa ccgcaccatc agctagcata accagttctt caggtgcatt gccatacgct
     1261 atgagcaatt cttttgcaat ccccaatata tagggcatat ctataccaaa aggacaaaac
     1321 atggtgcaac ggcgacagcc ggtacatgaa aatgctgctt cacgcagttc ttccataagg
     1381 ttttcatcaa attcatgtgc atcaccccac catgggaaaa attttcctgc tggcttaaaa
     1441 tattttctga aaaccctgcg gacaacctca gccctgccta ccggtgaata ctcttttttt
     1501 ggtataccct ggtaaacatg gcaggtatca taacacaaac cgcatcgtgt acagagatca
     1561 agataataca gcatgggtct gttcaacttt tttccaatga cctgtttaag ttccagtact
     1621 ttttcccttt ccatggttag ttccttttac ggtaatgtgc taaaaaagta aaaaccgaat
     1681 gtatcatttt gctaaatgga aacatcatga ggaagatgtt ggcaaaaaga acatgcagta
     1741 ccagtattgt atagtgcgat agttcagtaa tattatacga tagctggggt ttaaacaata
     1801 gcaaactttg gagccagttt ctgtatacat taatatcaac cctgagcact gcaccccaat
     1861 cgctgtaccg ttctgctaaa tggagtattg aaccaaaaag tatacaaaaa aacaaaacaa
     1921 gcaggatgat aaaatcttcc ggtatggaga tttcacggta gggagttcca aatcgtctga
     1981 agaggaaata cagtgttgta agcataagaa tgatgcccac taaaccgcca cccagaaaaa
     2041 ttctatgagg gataatctgg atgaatttaa aatcagctac taattccaga tgaccaagaa
     2101 aaagaaaaaa gaatgcaatg tgaaaaagca ttatgataaa ccagaaacct ttttgcttag
     2161 cgtatgccat cgggaccagc atagcgtcat agatgagtcc ggataccttt gaaccttttt
     2221 taggaaatat agctcctgta cccacaggag caggagcttt ccatatacta taaaatctat
     2281 agataagccc aaaaatcaag gttgcaaagg ctatgtaaac cataggaata agaataaaat
     2341 atgataaata gcctgacatt gttcacctca cagcattact gtgttatttt agctttttga
     2401 taaaaaattt tgtagtagta ccatctttca cagtatcaat gatctcatta ccactggttt
     2461 ttgcccatgc cggaaaatct gcaagagcac cgggatcggt tgtttctgcc attaagactt
     2521 gtccaacctg catcttttta atctgctgtg atacttttac aacaggtaat ggacaggcca
     2581 atccttttaa atccattgtt acatcagctt taatgtcact cataatttcc tccttaatta
     2641 attgttacaa tactatatac tatatactat atactatata ctataacatt aacaatacaa
     2701 taacttcccg tcattcttac ttatgtataa agtaataaca tatataagac aagaataaat
     2761 ttaaaaaaat ttttatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa
     2881 acataacagc ttccatgccc attgctacag aacctgttgc aataatgaag gaagctaata
     2941 gcttatcaag atcaccactg aatacaatca tagcaatctt atctttcctt ccatcctgaa
     3001 tctccttaat ttgattctgg atggattcta atgttttgtt aacatccata cattccccct
     3061 ggattatagt tttttgataa tttaaaattt tataataaac tttaatacta attaaactat
     3121 actacaattt tgtaaataac aaaaattttt ttattgaaaa ttaatatttt atgattctac
     3181 aatagctatg gattgtacta tacagtatac gtatagtatg tgcgatagtt ccatacaata
     3241 aaagcttaac attaatattt ttttactcta tgaatgtgca ttgttgacag ttgttgtaag
     3301 aagatgtacg gtggtgattt tatgtagcga tgctgctgca caatagtata tattgaataa
     3361 agagcacaca gtgaataaca aacctgttac taataatgaa gctcataatg acgacaatcg
     3421 acttcacttt gccagtacaa acgaagcact ctatgctttg aatatgtata tgaatagatt
     3481 tatgtggatt tcaacaagag agatagtttc cggaataagc tcatggtact ataatgacac
     3541 aaaaggggag aaactataaa ataataaggg gtacaccatg tcagacccct tattatttta
     3601 ttttaatgaa actattgttg ttattcagca tcatttgatt ttttacatgt gcttatcttt
     3661 agaagatcat atatccagca atagccgcta tatgctgaat ataaaacagc cagtcccaat
     3721 gccagtaaaa tgattgtacc tatagcgccc ggttttacaa taaaaatatt aattaataaa
     3781 gaaactccaa taagaactcg tatgtacctg tctgctgtac caacattttt tttcataact
     3841 cacctcggtt aatcaatatt aaactataat gaaatatcac aaagtaaatc ttttaacatt
     3901 aagtaaatat ggcaagtatt tttttatata tctgttataa aagataattg caatattgca
     3961 atttatagta ttatgggtac cgctaaaact atattttcat tattaaaaaa tagttggaaa
     4021 caataataaa gccttttacc aatttaccaa tgcttgtgtc cacacaaaaa tacactattg
     4081 tatcatttcg ggaagacaat tattacaggt aaaaggatag tgcagtttat gattgatatc
     4141 ataagtactt ttatatgttt ctgcttcatg atcacgcaat cgtattaaaa ctttttttga
     4201 cccatgagta aagaaaaata ttcgtgactc aacattggtt gtcgggtctt ttcgtatacc
     4261 ataaacattg ataaaaagag tattgttaaa ttcagggatg attttactat tctggcctat
     4321 ccatgcaggt aagtgtatgt gtgcacttat ccatatatca acgtggtact gttgtaaaac
     4381 tttctcaaat cgttctgagt ttatgattat atgctttttt gatactgtcc ctattaaatt
     4441 gctgtttttt aatggtgcat gcgttactgt tatgatattt ttatcctgat ttgtaataac
     4501 aatatttttc caccataaga aagtttcatc ggaaatatag gttggtggcc tgccacgttc
     4561 atttgacatg aatatgaaaa taatatttcc aaaaagtaca gtataacgta atggtttgcc
     4621 aatatatgta ttatatgctg gcatattttt ccattcgtga tttccggcaa tttcgtacca
     4681 atagttaatt tctgccttct ttcgtaattc tataaaccat tgataatctt cttctgcttc
     4741 tttgtgttgt acgatatctc ctgctataat ggcaaagata ggatgcggtt taatattcat
     4801 aatatcgtct atagcccttg cataatgcca tcgttctgat ttttttgtgg gttgaatatc
     4861 cgagtgtacc cagacggtat atacgccatc ggaaggaata tgctgtttat aacaggcaat
     4921 ggtgttgaag acgagtgcaa ttgctattat gatgactata cagtatcgtt tcatggtggt
     4981 tatatataca gtaactatcg tacatttcat gaaaaataaa gaaaaaaaat attgcaacta
     5041 ttacaatagt gtatcataat tgattcaaat ttaaaatatt ttagcagact atggataaca
     5101 atattactat tgatacatcc cgttttcagg agatatctgt ttcgtattta aaaaaattaa
     5161 gtggtgataa gcttttaacc tttgatgtat tggaccagca ggggaatacg gtattttctg
     5221 ccgggcaggc gcttgatgat gagttgttaa aaaataaatc tgaagaaggc ataaaatttt
     5281 ttattccccg tgttactgaa tcaattggtg tttatgaccg tgaaatcatt gacatgaaaa
     5341 agttaaaggc catagcagat gatacggcaa cgatgtatac ggatatacgt aaaaatggga
     5401 aaatgaccca tgctcagttt ttgcaatcac ataatcattt agcagccatt gtggacagtt
     5461 tgcgtcctga ggataaaacc ggtggagtgc tatcgctttt aaaagatata aaagactttg
     5521 attataccac ctatacccat tcggtcaatg ttggtttgct tgcaatgatt ttagtatata
     5581 aggagatgtc aaccggtgat aaggttaaat tggcagcgct tgcaggatat cttcatgata
     5641 tcgggaaact gcgagttgag caatctatca ttcagaaacc aggtctgtta tcacaggatg
     5701 aatttaaaaa ggtgctggaa catactcgtg aagggtatcg aattctggac agtgtcgttg
     5761 attcaacagg gaaaaaggtt gttcccaata gtataaaata tgttgcacta tttcaccatc
     5821 gtaaattgca aagtccgggc tatccattca gggaaaccga taaggacaaa acccttgaaa
     5881 atcaatacag tgaattgccc gatttagccc gtatgattgg tatatgtgat atgtatgatg
     5941 ccttaacaag tgcaacacca ttcaggattc ccatgggtgt tgaaaaagct ttacgatata
     6001 tattaagttt atccaattac ctgtattcaa ctgatgacat ccataggttt attaaggtac
     6061 tggggctatc cttaaataag ggtactccat ttttaaaagc aggggatttc atcatgcttg
     6121 aatctgaaaa gataagcatg gatacacgca aaaaggtaag agtttatgag gtagcacgga
     6181 ttgaggaaat ttcacggata aacttcatga atcctaaggt tatgatattt tatgatacaa
     6241 taaaaaacaa aaaggtacat aatattattg tggatttacg gtatgacagc aatcgcaggg
     6301 tggtgcgcgt ttttgagagc acacgaatac gcaatatgct attgagacag cttaagtagg
     6361 gtagaaagta tgaatccaat tttattaata tttgataatg accgttttgc tgaatttata
     6421 cgctttgtat taacaactgc tggcttaaaa attgaattat tagccgatcc actggaaatt
     6481 tttgaaaagg ttaaagagat gaatccacca ttaatagtta tggatatttt tttaaaaaac
     6541 attgatggct tatatttact ggagcgatta tattctgtta aagaatttac aaaaataccg
     6601 gtttttattg tatcatcccg caacgaacct ttagctgttt ttgatgcatt agagcgagga
     6661 gctgctgatt atatggcagc accaatcaat gaagatatgt taattgaaaa ggtcaacaag
     6721 ttgttaaaaa ccaattgaaa gccaattggg atccaattga aagctaattg aaacaaaaca
     6781 gtattgaata aagccgtaat gtaccgacat atataatgaa gtgtttttgt aattttaatg
     6841 acaaatgggg agtttctact attctatttt cagggatttg ttatgaaaaa atgtatgcta
     6901 ttagtgctta tagcgcctat tttcttatta cagtgttcat cacaaattaa tctctatgat
     6961 acatcatcaa atggacaatc aaataacacc atcaatgcgt atacaatggc agaaagcctt
     7021 tcatttaaag aggtatgggc atatcttatg aaaggtgaag aggataagct gaccggtgag
     7081 gaaaaaatta ccgatttgtg ttatttcagt gctaaggtaa acggcaaagg acaaattaca
     7141 gacgattacg caccacctgc aataaaagct gaaaatagaa tacgccacca tatcgttata
     7201 gcaattcttg ataattatgc actaacacat tttataatac atcctgattt taaggtgcga
     7261 aaaggtttta ttaaggatat ttgccggatt tcacaaaaat ttgatggagt tcagattgat
     7321 tttgaatccg ttgcttcatc agatgctcac tattttttta gctttttatc agaattacgc
     7381 gccaatctgg gtaaaagcaa ggtagtatca attgctttgc cagcacggag aagccgcatt
     7441 gcggatgcgt atgattatcc ttctattgca gcactggtgg ataggattat catcatggca
     7501 tatgatcagc attggtatgg tagtaaccct ggtcctattg cagggctatc atggtgcaga
     7561 gaggttggtg aatttgcaaa gagctttata ccgcagcaaa agctaatcat gggtttgccg
     7621 ttatatggac gatgctggca ggataaaaat tttaataaat caatgcatta tacccatata
     7681 gaacaggtta tgcaaacatc caatatccag ccccggtatg atcctgagaa tggatcatat
     7741 tttgaatatg aagaaaagat taaagtgcgc gtatatttta atgatgaacg tgcattatac
     7801 gaaaggctaa agctgtactc atcgatgtct attgacaatg tggcattctg gcgtataggg
     7861 cagggaccaa aagggatgtg gcagggtatc agcacaaaaa gtttataaca atgttacttt
     7921 atgaaaaaaa gtaatgaaga aattatccta cgacgatata cagagcaatc gtcccgataa
     7981 aaatgaatta ttcagattac agcggtttcc ggttgtaacc attctggaaa atattagaag
     8041 cctgtataat gttggctcca tgttcaggac tgctgatgct gcgcgaattg cctacatgta
     8101 tctctgtggt tatactccac atcctccacg aaaagagatt gaaaaaactg cgctgggcag
     8161 taccgaaagt gtgccgtggg agcattctac caaaccaatc cagataatac ggatgttaaa
     8221 aaatcagggt tttgtgattg cagcactgga gcacacaaca acaggtgtag cgtatacaac
     8281 ctataattat tcctatccac tgtgtattgt tgttggcaat gaggtgcatg gtgtaagcga
     8341 tgatgtactg gctatgtgtg atgcggctat tgagatacca atgtttggca tcaaacattc
     8401 gctcaatgta gcggttgcgt atggtattgt ggtgtatcat gccgttatga catatcagat
     8461 gaagtagtag cgcagtataa tgactatgca aaaagtaatt ttaaaacaag gaaaagaaaa
     8521 atcagtactg caaaggcatc catgggtttt ttcaggggcg atagcaactg ttgatgcaga
     8581 tgatggtgca atggttcctc tgtgttcata tgatggtact gtgattgcat ggggatatta
     8641 caacagccga acacagatag ccatgcggct attaacattc agcgatacaa aacctgatga
     8701 tgatttgatt gcccgacgat tgatgaacgc gtatgcatta agggttgaat ccggggttgt
     8761 aaagagtacc aatgccttca ggatggtgca ttctgaagga gatatgctgc cgggactcat
     8821 tgctgactgg tataatgggc attgtgttgt acagttttta acatcaggga tggatcgctt
     8881 aaaaggaact atcgtgcaga ttatacagga gaagctgcag ccgnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnngatagct ccggatatgc tctttcaatt gcacaaaaaa
     9061 attgtgaatt aaataatgtt ggcaaacggc atgcaggtgt aaaggcggat gtttttgatt
     9121 atatcaatac caatgatatc ccccagaatg taatcatatg tgatcctcct gcgcttatta
     9181 agagtaaatc ggcaatacag cagggattgc gcggttataa agaattaaat gtaaaaattt
     9241 ttaaaaaggt gcctccgctg tcatttgtat taacatgttc atgctctcgt tttgttgata
     9301 tgaaattgtt tcagaaaatt gtgttttctg ctgcacgtga tgccggccgt gaggttacta
     9361 ttaccgggaa atatctacag cctgccgatc atcctgtcag tatatattgt cccgaaacag
     9421 aatacctcaa agccattatg gtatttattt catagtaact atcgcccgca gagtattttt
     9481 taaaattttt attgacaaac ataaaatata ctactatatt ggtagtaatt aaggattgag
     9541 caattggcga tagagagtaa caacgattgt gtgctgattt tgcgttgtga tgtattgtat
     9601 aatgcgcggt aacaggagat aaccatgtgg gaatataccg acaaagtaaa agaattatat
     9661 accaacccta aaaatgttgg tgaaataccc gatgccgatg ttgttgttga ggttggtagt
     9721 attgtgtgcg gcgatgctct gacactgtat ttgaagcttg acggcgatgt aattgtagat
     9781 gctaaattta aaacatttgg ctgcggcagt gcaatagcgt cgtcctcggc actaaccgag
     9841 atgataaagg gtaaaaccat tgcagaagca gcaagaatta ccaataagga tatagttgac
     9901 tttttaggtg ggcttccgga ccaaaaaatg cactgctcgg tcatggggca ggaagcactg
     9961 gaaaaagcca taaaaaaaat aaaaggtgaa accattgaag atgaacaaga agatgaggga
    10021 gcggttatat gccagtgttt tggtgttact gaaaatgtaa tacgtcgggt tatcagggaa
    10081 aataatcttg ccacggttga ggatgttacc aattacacca aagctggagg tgcttgcggt
    10141 tcatgtatac caaaaattga ggacattctg aaagaagaat tagccagcgc tgtagcgcaa
    10201 ccagcagcaa ctgccatgaa gccaatgtca aatataaagc gtatgcagct tgttgaagag
    10261 acaatacaca acgttatacg ccctgttttg atgagggatg gcggtgatat cgaccttgtt
    10321 gatattgaag gaaaaaaagt atatgtagcg ttacgcaggc actgtgctca ttgtgttgtt
    10381 tcagatgtta ccatgaaaaa tctggttgaa gatacattaa aagagtttgt agaaaacgac
    10441 atcgaagtga tagaggtaaa gtgatgaata ccattatata tctggataat aatgcaacga
    10501 ccatggttgc acctgaagtg ctggaagcta tgttgccgtt ttttacccgg cgctatggca
    10561 atccatcaag catgcatgat tttggtggtg atgttgagca tgatatggaa aaagctcgca
    10621 atcaggtggc acgcatgctg ggtgcggaat atgattatga gatagttttt acaagtggtg
    10681 caaccgaaag cgataacatg gcaattctgg gaacattgca gtactaccgt gataagaaac
    10741 acatcattac aactaaggtt gagcatccgg cagtattaaa tttatgcaga cagcttgagc
    10801 gcgaaggata tagcgttacc tatgttcctg tagacaggga aggcaacctt gatgtggatt
    10861 ttctttttga ttcggttacc gatgatactg ctattgtatc ggttatgtac gccaacaatg
    10921 aaacgggtgt aatcttcccg gtggaaacaa ttggcgcttt ttgcaaacag cgcgggatta
    10981 cattccatat tgatgcagta caggcaattg gcaagattcc tgttgatgtg aatgcattgc
    11041 aatgcgattt gctggcaata tcagggcata agtttcatgc acctaaaggg attggagtgc
    11101 tctatgttcg aagaggtaca cggctacgac cgttactgta tggtggacat caggaaaaag
    11161 cccgcaggcc tggcacccat aatgtgcccg gtattgttgg tattggcaaa gcagccgaac
    11221 tggtaatgca gcatctatca aattctgaag aggtggctaa cgttaaacgt ctgcgcgaca
    11281 gattagagaa tggcttgctg tcgcaattga gcaatgcgca tttgaacggg aataaaaaca
    11341 acagggtgga taataccacc aatattggct ttgaatttat tgaaggggag gcaattttat
    11401 tgtatctcaa cgaaaagggg attgctgctt cttctggctc tgcctgttca agcggttcac
    11461 ttgagccatc acatgtgttg cgtgcaatgg gggtgacgtt tacctctgct catggttcta
    11521 tcaggtttag cctcagccgt tataccactg aagatgagat acagtatacg cttcaggtaa
    11581 tgcctgaggt agtaaacagg ttattggcaa tatcgccata ttgggatgca atgaataaaa
    11641 aaggcaagtc catgacagaa cttgcccatt gatagtataa gattattaaa aatatagttt
    11701 accgtgcaac ctcattgata agccttccca gttcctgatc gtgggttagc attgcttttt
    11761 gattggcttc atagcttcgc tgtacttcaa tcatatccac catctcgcgg acggcattta
    11821 catttgattt ttcaaggaat ccctgtataa ccttgtattc ctcggctggc aatgccggtc
    11881 cagaatattg tgtttcatgg tataatgatt cgccttcttt ttttaactca cggatgtttt
    11941 caaaatcaac aatttttagt ttgtcaataa caaccggctg ctcccagtta ttctgggtta
    12001 gcccaatcat atcatcagga tttaatgata atgcagagtt aaccaggatt tcaccccgtt
    12061 cattgatcat aaaattgttc ttctgtaatt ttatgatgcc attttgaccc aggacaggca
    12121 atccactatg cgttaccagt attccatctt tattcagggt aaaagcgcca ttacgggtat
    12181 atcgttcgcc acgctcggtc atgacagtga agaatccatt accctccatt gccatgtcaa
    12241 atggattttc agtacgcatc agcgagccct gttcatggat agtatatacc tcgttcactt
    12301 caacaccggt acccaattta ccaacaaagg gcatggtatc atacgaacca gccggagtaa
    12361 tacccagccc atcatcgtta aggcggcgga taatcatatc agggaatgct ttaaaaaggg
    12421 taaggtcttt tttatatgct gctttatcaa catttgccag attattggca atagcatcaa
    12481 gacgtgcttc ctgtgcaatc ataccgctgg cacctgtata gagtcctctc accattatgg
    12541 ttttcctcct tttactctaa ctatatcggc acctaacgcc tgtaactttt gctcaaaatt
    12601 ttcatatcca cggtcggtat ggtaaatacg gcggatttcg gtttttcctc tggcagctaa
    12661 cgcagccagc accagagaag cgcctgcacg cagatcggac attaaaaccg gagcagcctg
    12721 caattgctta ccacctttga taatagcagt atttccttct atttcaatgt cagcacccat
    12781 tcgccgtagt tcgccaacat gggtataacg gttttcaaaa atggtttcaa taatgacact
    12841 tataccgggg atagtacaca taagcgacat gattggcgcc tgaaggtcgg tagggaagcc
    12901 agggtaggga agagtgcgta ccatactgcc ctgcaatttt tttgacggtt caatggtaac
    12961 ggagctatcg ttacctgaaa taacaaaacc catgtcggca caggtatcaa tacaggcctt
    13021 aacatgttcc ggaataacat tattaacggt tacattacca cgggtgatag ctcctgcaat
    13081 taaaaaggta cctgcttcta tgcggtcagg tattatggag tagcttaccg gcttcagttt
    13141 ttttactcca ttgatgatga tgcgctctgt ccccagtcct tcaatctttg ctcccattgt
    13201 tattaaaaaa ttgcccagat caacaacatc aggttccatg gcgcagtttt caaggattgt
    13261 ttgtccatct gcaagcacag cggccatcat gatgttggca gtggcgccaa cggaaacctt
    13321 ccgcattata aaacgattgc cgtttaattt ttcaacacgt gctttaatat agccatgctc
    13381 aattgttatg gtagttccca gcgcttcaaa acccgataga tgaatatcca ccggacgctc
    13441 gccaattgcg caaccgcccg gcaacgacac gtctgcctcg ccgcatcgtg ctaaaagcgg
    13501 acccataaca tagattgaag cacgcattgt tttcacaaga tcatagggca cttcagcatt
    13561 ttttaatgag ttaacatgaa tggttaacgt attggtattg gtgtcaaact gatattgtgc
    13621 gcccagacat tcaatgacct gtaatattgt ctgaatgtcc tttaaattag gtacattgtg
    13681 aagaactgtt gtaccttctg caagcaagct tgcgacaatt attggtaaag cggcattttt
    13741 agctccggat atggttaccg tgccattaag tttgttgccg cctttgacta tatagaagtc
    13801 cacagttatg cctctttttg tactggttta gtgatatgct attgataggt tcagtatgga
    13861 tggtttgcta aattgtcaat tcaatt
//
LOCUS       DAOSOX010000048        13848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4448_length_13848_cov_8.922279, whole genome shotgun sequence.
ACCESSION   DAOSOX010000048 DAOSOX010000000
VERSION     DAOSOX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_13848_cov_8.922279"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /gene="glgX"
                     /locus_tag="PLX22_05210"
     CDS             complement(<1..1612)
                     /gene="glgX"
                     /locus_tag="PLX22_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOT19329.1"
                     /translation="MQTTKQYPFVTLPGYPLPLGVHIDRHGAQFAIFSRHATAISILL
                     FESSETEEYIEIELDPSMNKTGDIWHIWIAGIREKQLYGLKVDGPYEPIQGHRFNKHK
                     LILDPYANAISGNHIWDFSKACGYIKGHPDMDLSFSTEKNYKESPKCLVVDTQFDWFD
                     RPINIPMEDTIIYELHVKGFTWHSSSHVANPGTYKGLTQKIPHLKELGITAVELMPIQ
                     EFDEFENININPFTGERLKNYWGYSTIAFFAPKSSYSSSGTMGQQVNEFKEMVRDFHQ
                     AGIEVYLDIVFNHTAEGDHLGPTINFKGLDNSIYYMLKEDRRFYHNFSGCGNTVNCNH
                     PLVRDFILDCLRYWVIDMHVDGFRFDLASILGRDQEGNILQNPPLIEKISEDPILRNT
                     KIIAEAWDAGGAYLIGHFPGRWAEWNGKFRDDVRHFWRGDNDTVPTFMQRIIGSPDIY
                     LRSNRSPLHSINFITCHDGFTMLDLVSYNEKHNLANGENNLDGDNNNISHNYGVEGHD
                     APQAVQRIRRQQIKNFFTTLILSQGVPMV"
     gene            1937..2739
                     /locus_tag="PLX22_05215"
                     /pseudo
     CDS             1937..2739
                     /locus_tag="PLX22_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2872..3780
                     /gene="pstC"
                     /locus_tag="PLX22_05220"
     CDS             2872..3780
                     /gene="pstC"
                     /locus_tag="PLX22_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT19330.1"
                     /translation="MLKERLKIALNIHTASDRIIRYIFAVVGFVSVAFLFLIMIFLFK
                     EGLPVFNSVSVKEFLFGTSWYPTSVPPKFGTLPLIVASLTVTFFAAAFAVPFSMAIAI
                     YLSELAPATLREIVKPIIELIASIPSVIIGFFGMVVVAPFLQDHFDIDTGLNVLNAAI
                     MLAFMAIPTIASISEDALSSVPASLKEASYALGANRWQTIVHITMPAALSGVWTAIIL
                     GISRVLGETMVVLMVAGGATIFPTSLFDPVRPLTANIAAEMAEASYRSDHYFALFAIG
                     IILFVLTLLFNVIANYLSMKYKFKWN"
     gene            3785..4651
                     /gene="pstA"
                     /locus_tag="PLX22_05225"
     CDS             3785..4651
                     /gene="pstA"
                     /locus_tag="PLX22_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT19331.1"
                     /translation="MATLQQKAKYYETSSFVIITILSYAIIGFLLFVLGYIFLQGYKA
                     LSWDFIVSFPKNEMREGGIFPAIIGTLYLMIGSSLVSVPIGIMTAIYLTEYVENVTVL
                     NFIRLGINNLAGVPSVVFGLFGMSVFVIFLDFGSSIIAGSLTLGILNLPLVIRSTEEA
                     LLTVPMSYREASLSLGATRWQTIYKIVVPTALPGILTGIMLSLGRAAGETAPIMFTCA
                     TFYTPFVPDSLFSEVMALPYHIYVLATAGTHIEATRPIQYGTAIVLIVLVLGLNLAGM
                     IMRYSYRKKLKG"
     gene            4752..4970
                     /locus_tag="PLX22_05230"
     CDS             4752..4970
                     /locus_tag="PLX22_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19332.1"
                     /translation="MKNANKIYMDVCSLCRPFDDQHYLRIRLETNAVNLILSKVKDSH
                     LELINSPVLIKEIESIDDDLERTELCNY"
     gene            5199..5396
                     /locus_tag="PLX22_05235"
     CDS             5199..5396
                     /locus_tag="PLX22_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19333.1"
                     /translation="MRASRYLSEKEMLQKGIDVLLDALGPVETVRFLNISRDKRIESV
                     KRHRMWQKSLKKDDFINEIVK"
     gene            complement(5475..7211)
                     /locus_tag="PLX22_05240"
     CDS             complement(5475..7211)
                     /locus_tag="PLX22_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19334.1"
                     /translation="MKRLKTKLVVVYTVALVIFISFLLMYINHHIHSAFIAFQEAELQ
                     QNIENIHIYLHSYHKPFNYVLFQNHDVVASLKNVSYKSSLRITIITDDGTVYFDSFAN
                     PELMENHLYRPEILQSRTKGVGSAIRYSKTVKSDMLYVAKYFDNYYIRTAKSLGAINI
                     LIGALTKNILIVGFAVLVLTIAANYIFATIFTKPIAESLQFIDQFFHGDLNTRILNYK
                     DDEMGYLQNSMNRLADNIQARINELTAQKNKLFMIIESIHDPIALIDDSKKITVYNKA
                     FIELFQFPETVTNTYYFSIIRHSQLNAKIHVALSQRQNFSFEETIGSNHFQIFIYPFP
                     QPDSGILLLMHDVTEQKRIQKLKSDLVGNLSHELKTPISIIRGYLETIKDISLDEKTA
                     GQFIDNALANLERQNAIINDMLKLNELETTAYAVTEYIDIKTIITRCIELLTPKIQKK
                     NLAIVMDIDAIPETIAGNAFLTEEIFFNIIDNAINYNNPNGAITIKSAVKTATISISI
                     ADTGVGIPQDEIPRIFERFYRVDKSRSRDTGGTGLGLSIVKHAAQILGWNVSVTSSMN
                     GTVFAITIPWAG"
     gene            complement(7240..7923)
                     /locus_tag="PLX22_05245"
     CDS             complement(7240..7923)
                     /locus_tag="PLX22_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT19335.1"
                     /translation="MSKIYVVDDEKDIREILKVNLQKNGYDVNTFASAEEVLKQLAIA
                     KPDLFILDIMMNGMDGLDLCKHIRASRELTTIPILFLSAKSAELDKVLGLELGADDYL
                     TKPFSIHELIARVKAIMRRSQTKQELSPKEILTYNAISLYPDKFQVTIDNQNIDLTKT
                     EFLILKLFMQYPGKIFSRDNIIDSIRGDNVYVIDRTIDVHIMNLRKKLGTYKDAIKTY
                     SGVGYGLKV"
     gene            complement(7927..8580)
                     /gene="phoU"
                     /locus_tag="PLX22_05250"
     CDS             complement(7927..8580)
                     /gene="phoU"
                     /locus_tag="PLX22_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT19336.1"
                     /translation="MSTHLEQELNELIRKILEMADIAIEAVEDSVQSLKNIDVKLAEK
                     VIQKDTALDTLEVFIDEECVRILVTKQPAAADLRLILALFKSNTDLERIGDFATNIAK
                     ETIRLEGKSLLKPLIDIPRMEKICIDMIKLAFRAITEKNIDYAKRCIDMDKEVDELNM
                     QINRELFSFMVENPATISPAFGLITVARALERIGDHATNIAERAVYYIQGEDIRHQE"
     gene            complement(8605..9369)
                     /gene="pstB"
                     /locus_tag="PLX22_05255"
     CDS             complement(8605..9369)
                     /gene="pstB"
                     /locus_tag="PLX22_05255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT19337.1"
                     /translation="MSEKEIKVQAKNLSFYYGSSQALFDIDLTVLANSVMALIGPSGC
                     GKSTFLRCINRMNDIIPYTHIAGDMTIDGESIYTIKYDVTQLRRRVGMVFQKSNPFPK
                     SIFDNIAYGLKINGIKDRYIIEKTVEDSLKRTALWDEVKDRLNDSAMSLSGGQQQRLC
                     IARTIAVQPEIILMDEPASALDPISTQKIEELIQDLKKQYTIIIVTHNMQQAARVSDY
                     TAFFYMGKLIEVNKTEVMFTKPDKQMTENYITGKFG"
     gene            9935..10762
                     /locus_tag="PLX22_05260"
     CDS             9935..10762
                     /locus_tag="PLX22_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT19338.1"
                     /translation="MKVKSFLVLMITAFVFSVSMGFSKGNDAKVVIKGSTTVLPIAQK
                     AVEVFYDKTKIIISLSGTGSGDGIKSLIDGSCDIANSSREMHEVELEMAKRKGKTIKE
                     VAIAYDMIVPIVHPSNPVKNLSLEQLKAIYEGTITNWKEVGGNNEKIVVVSRDSSSGT
                     FEVWESKVMKKAEVRKDALLQASNGAVLSVVARNKKAIGYVGYGYLDKTVKGIYVNNV
                     EPTIPNGKSGKYPIARKLYMYVNDVKIKPEAKQFINFILSNEGQIIVKEAGFIPIID"
     assembly_gap    10960..11059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11060..11281
                     /locus_tag="PLX22_05265"
     CDS             <11060..11281
                     /locus_tag="PLX22_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT19339.1"
                     /translation="YGYLNDTVKVLFVNSVEPTIPNGKSGKYPIARKLYMYVNDVKIK
                     PAAQNFIDFILSPEGQSIVKETGFIPIID"
     gene            11588..12391
                     /locus_tag="PLX22_05270"
     CDS             11588..12391
                     /locus_tag="PLX22_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOT19340.1"
                     /translation="MITYIAILFVYEDTAVSQEKAVKQIYLSSNLKKKGLVEVREGIA
                     FVKIPAGSFMQGGNKVKVNGFWMAMCEVTQQQYESIMGINPSYFKGNPDNPVERVNWY
                     NAVEFCNKLSIKAGCKPYYTIDKVHKDPENKNTGDTIKWTVTINGGNGFRLPESAEWE
                     YACRAGTTTRYYWGDNVNGDYCWYNANSDEQTHPVGTKLPNAWGLYDMSGNVWEWCFD
                     WDIDDYGVFRIIRGGSWNYDADLMESSVCNYGYPNDTFRILGFRPVRNL"
     assembly_gap    12495..12594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12718..13788
                     /locus_tag="PLX22_05275"
     CDS             12718..13788
                     /locus_tag="PLX22_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT19341.1"
                     /translation="MKNICTIQAGAKAYAIIKDEGIQKERIRCMLGAAGGPKWIILYG
                     LDKALYKSFLQKRKNPVYMIGSSIATWRFAALSLADGLQGIEAFKERYFAQKYSMKPD
                     REEITAETVAILEEFLPDSKVDEIVKHPTQRLCIITARSRHLLQYEDTFRLGAGLGIN
                     AITNMVSRKLVGLHFDRFAFYDAREFPSFIQWKNYHTQYIPLNNNNLKKAVLASGSIP
                     LVMNGVVFQHEGKTHILRDGGLVDYQQDLPLEPGDYIALYPHYSDRIIPGWFDKPLKY
                     RKPHHALENVCMVSPSKEAIASLRYKKIPDRKDFYTFAGDDSRRLLFWNETVALGQQI
                     AEEFMELVESGRIKDRVIPLAR"
BASE COUNT         4166 a         2314 c         2741 g         4427 t
ORIGIN      
        1 gcaccatggg gacaccctgt gacaatataa gtgtggtaaa aaaatttttt atctgctgcc
       61 ttcgtattct ttgaacagct tgtggcgcat catgtccttc aaccccatag ttgtgactga
      121 tgttgttgtt gtcgccatca aggttgtttt cgccatttgc aagattatgc ttttcattgt
      181 atgaaacaag gtcaagcatg gtaaagccat catggcaggt gataaagttt atgctgtgta
      241 aagggctgcg gtttgaccgt aaatagatat cggggctgcc aataatccgc tgcataaagg
      301 taggcacggt atcattatcg ccacgccaga agtggcgaac atcgtcgcga aatttgccat
      361 tccattcagc ccatctgcca gggaaatggc ctattaaata ggcacccccg gcatcccatg
      421 cctcggcaat gatcttggta ttacgtaaaa tgggatcctc ggatatcttt tcaatgagcg
      481 gtgggttttg cagtatattt ccctcctgat ctctgcccag tattgaagcc aggtcaaatc
      541 tgaaaccatc aacatgcata tctataaccc agtagcggag gcagtctaaa ataaaatccc
      601 gcacaagcgg gtgattgcag ttaacggtgt taccgcatcc ggaaaaattg tggtaaaatc
      661 gtctatcctc tttcagcata taatagatag agttgtccag ccctttaaaa ttgatggttg
      721 gaccaagatg gtcaccttcg gctgtatgat taaaaacaat gtctaaatat acttcaatcc
      781 ccgcctgatg gaagtcgcgt accatttctt taaactcgtt gacctgctgc cccattgtcc
      841 cgcttgatga atatgaagat tttggagcaa agaaagcaat tgtgctgtaa ccccagtaat
      901 tttttaaacg ctctccagtg aatggattaa tgttgatatt ttcaaattca tcaaactcct
      961 gtatgggcat aagctccaca gcggttattc ccagctcttt aagatgggga attttctggg
     1021 ttaatccttt gtatgttcct ggattggcaa cgtgtgacga tgaatgccag gtaaatcctt
     1081 ttacatgcag ctcatagata atggtatcct ccatggggat attgatgggt ctatcaaacc
     1141 agtcaaattg agtatcaaca actaaacatt tgggtgattc tttgtaattc ttttcggttg
     1201 aaaatgataa atccatgtca ggatgtcctt tgatgtaacc acatgccttg ctaaaatccc
     1261 atatatggtt accgcttatt gcatttgcat aggggtcaag gattaactta tgtttgttaa
     1321 agcggtgccc ttgtatcggt tcatacggtc catcaacctt taaaccgtag agttgttttt
     1381 cgcgtatgcc agctatccag atatgccata tatcccctgt tttgttcatt gacgggtcaa
     1441 gttcaatttc aatgtattct tccgtttcag atgattcaaa caaaagtatt gaaattgctg
     1501 ttgcatgacg gctgaagatg gcaaattgag caccatgcct gtctatatgt acacctaagg
     1561 gtaatgggta tcctggaagg gtaacaaaag ggtattgctt tgttgtttgc acgatagttc
     1621 ctgtcaatgt ggtcagtatt gtttgcgcta aagcttatga taaatagtat tgcatgcttt
     1681 tgtttagcgt caattaaatt atttaactta accatagaaa taatgctatt gctatggagc
     1741 aaaggtttgt tattgttcta tgcaatagaa aaacaaaaag tgctatagtg tcgttacaat
     1801 attgcattac agcgtggaaa ggcgcgagaa taaagaaaat acttttgcca ttatggttat
     1861 aacaatattt catttgacta aatgccctat tatttttata cttgaagaaa tagtaccata
     1921 agggctggct ttataaatgg ttactatata ttttcagcat caagggttaa aaattatcaa
     1981 tgatgatgtt ttgacgacaa catcaattga taatagtagt attgattgta ttgttacctc
     2041 accgccgtat aatgttgcca tacagtatgc aacccatagg gatacgctac aatacaatga
     2101 gtatcttgca tttagcagaa agtggttata acactgctat gcatggttaa aagatgatgg
     2161 aaggttgtgc ctcaatatac accttgataa gaataaaggt gtgcagcaaa gtgtgggtgc
     2221 agatttaacg gtaatggcca tggatattgg gtatcaatat cattcaacaa ttatcttgaa
     2281 tgaaggcaat atttcacgac gaaccgcatg gggttcatgg ttatctgctt ctgcgcagta
     2341 cgtgattgca cctgttgagc tcattgttat attatataaa aaaaatggaa aaagacatca
     2401 ggttctagaa agtcggatat aacaaaagaa gaatttatgg aatggacaaa tgggttatgg
     2461 acattttccg gtgaaagcaa gaaacgtata ggccatcccg caccatttcc aattgaatta
     2521 ccaaaacggt gcataaaatt attttcattt atcgatgata taattttaga cccatttatg
     2581 gggtcaggaa caacgcttat tgctgcctat aacttaaaaa gaaatgcaat agggattgat
     2641 ttagatactt catattgtgc attagcaaaa gatagaatca ttaaagaatg tactatatat
     2701 caggatgatc tatgtaaggc tgtaaattat gagaaatgac actatatctg atatcatttt
     2761 agaatatttt taacgttatc ttcataaaat atttataatt tctttacata gtggtgacta
     2821 atttaaagtt gattatcttg attttgatta taccatttac actataatat catgctcaaa
     2881 gaaagattaa aaatagcttt aaacatccat actgccagcg acaggattat acggtatatc
     2941 tttgctgttg tagggtttgt ttctgttgct tttttgtttt taattatgat atttcttttt
     3001 aaagagggat tgccagtatt caatagtgtg agtgtgaagg agtttttgtt tggtacaagc
     3061 tggtacccaa catctgtgcc accaaagttt ggcacattgc cattgattgt tgcatcgttg
     3121 acggtaacat tttttgcagc agcttttgct gtgccattta gcatggcaat agctatatat
     3181 ctttcggaat tagctccagc cactctccgt gaaatagtca aacccataat agagctcatt
     3241 gcatccatcc catcggtcat tattggtttt tttggcatgg tggttgttgc cccgtttttg
     3301 caggatcatt ttgatattga tacaggactt aatgtattaa atgctgctat tatgcttgcc
     3361 tttatggcca taccaactat cgccagtatt tctgaggatg cgttatcgtc cgtgccagca
     3421 tcactaaaag aggcatcgta tgcattaggt gctaacagat ggcaaacgat agttcatatc
     3481 accatgcctg cagcgctttc gggggtatgg accgctatca ttctgggtat ttcacgtgta
     3541 ttgggtgaaa caatggtggt actgatggtt gccggtggag ctactatctt tcccacatcc
     3601 ctttttgatc cggtgcgccc cttaacagca aacatagctg ccgaaatggc agaggcgtca
     3661 tatcgcagcg atcattattt tgcgctgttt gccattggta tcatactctt tgtactgaca
     3721 ttgctgttta atgtcattgc caactatctt tccatgaaat ataagtttaa atggaattaa
     3781 tataatggca acgttacaac aaaaagcaaa atattatgag acctcttcat ttgttatcat
     3841 tacaatactg tcatatgcaa tcattggttt tttactgttt gtattgggat atattttttt
     3901 gcagggttac aaagcgcttt catgggattt catagtaagc ttcccaaaaa atgagatgcg
     3961 tgaaggcgga atatttcctg ccattatcgg tacattgtat ttgatgatag gctcatcact
     4021 tgtatccgtt cccattggta ttatgacagc aatctattta accgagtatg ttgaaaatgt
     4081 aacagtactc aactttatac ggctgggtat taataattta gcaggcgttc cgtcagtagt
     4141 ctttggtctt tttggaatga gtgtatttgt tatatttctt gattttggtt catccattat
     4201 tgccggctcg ctaacgctgg gaatacttaa tcttccgctt gtcatacgct ccacagaaga
     4261 agctttactt actgttccca tgtcatatag ggaagcatca ttatcgctgg gtgccaccag
     4321 gtggcaaacc atttataaga tagttgtgcc aactgcactt cccggcatat tgaccggcat
     4381 catgctttca cttgggcgtg cagcgggaga aacagctccc atcatgttta cctgtgccac
     4441 gttttacaca ccgtttgtac cggattcgct ttttagtgag gtcatggcat tgccatacca
     4501 tatctatgtt ctggcaactg ccggcaccca catagaagct acccgcccta tccaatatgg
     4561 aacagctatc gtgctgatag tccttgtact tggacttaat cttgccggaa tgatcatgcg
     4621 ctatagctat agaaagaaat tgaaaggata aatgaaatat tttttattgt aagcatatac
     4681 ctgtattcca aaacagtgaa tgaagctaat tcattgttga cataattttt acttagtata
     4741 ttatgtttgt gatgaaaaat gcaaataaaa tatatatgga tgtttgctca ttatgtaggc
     4801 catttgatga ccagcactat ttgcgcatac gtttagaaac aaatgcagtt aatcttatat
     4861 tatctaaagt aaaagattca catcttgaat taattaattc ccctgtgctt ataaaagaaa
     4921 ttgaatcaat tgatgatgat ctggaaagaa ctgaattatg caattattaa ataaatatgg
     4981 taaaatctat aatttcaaag ctgatatatt gaaaacagaa ttgaaacatt aatatcattg
     5041 cgttttggta tagccgatgc cgcacatatt gcatttgctg aatattataa tgctatattt
     5101 attacatgtg atgacaaatt attgaataaa tgtaaaaaat atattaatat tatcccatca
     5161 tataatcctg ttatattttg cgagaaggag aaattaaaat gagagcatca aggtatttaa
     5221 gtgaaaaaga gatgttacag aaagggatcg atgttctgtt agatgcatta ggtccggttg
     5281 aaacagtgcg cttcttgaat atatctcgtg ataaaaggat cgaatctgtt aaacggcata
     5341 gaatgtggca gaaaagttta aaaaaagatg attttataaa tgaaatagta aagtaaattt
     5401 agtaagggca acatactcaa catactcaac atactattga aacaaagcag ggtataaact
     5461 aattcatgaa agaattaccc tgcccatggt attgttatgg caaacaccgt accattcatt
     5521 gatgaggtaa cgctaacatt ccatcctaaa atctgagctg catgtttaac aattgaaagc
     5581 ccaagccctg taccgccagt atcacgtgaa cggcttttat caacgcggta aaatcgctca
     5641 aagatacggg gtatttcatc ctgaggtatt ccaacacctg tgtcagcgat gcttattgaa
     5701 atagttgctg ttttaacagc gcttttaatg gtgattgctc cattaggatt attgtaattg
     5761 atggcattat caataatgtt aaaaaaaatc tcttcagtta agaatgcatt gccggcaata
     5821 gtttcaggga ttgcatcaat atccataact atcgccagat ttttcttttg tatttttgga
     5881 gttagcaatt caatacaccg ggtaataata gttttgatat caatatattc agtaactgca
     5941 tacgcagttg tttccaattc attgagcttc agcatatcat tgatgatagc attttgccgt
     6001 tcaagattgg caagtgcatt gtcaataaat tggccggcag ttttttcgtc cagggagatg
     6061 tcctttattg tttcaaggta accccggata atgctgatag gtgttttaag ctcatgtgac
     6121 aggttgccaa caagatcaga ttttaacttc tggatgcgtt tttgctcggt tacatcatgc
     6181 atcaaaagta agatgccact atcaggctgg ggaaaagggt agataaatat ctggaagtga
     6241 ttgctgccaa tggtttcttc aaaagaaaaa ttttgccgtt gtgatagtgc aacatgaatc
     6301 tttgcattga gctggctatg gcgtataatg gaaaaataat aggtattggt gacagtttcg
     6361 gggaactgga acagttctat aaaagcttta ttgtatacag taattttttt actatcatca
     6421 atgagcgcta ttggatcatg aatgctttca attatcataa agagcttatt cttttgggca
     6481 gtgagctcgt ttatgcgtgc ctgtatattg tcggctaatc tgttcatgga attttgtaga
     6541 tagcccatct catcgtcttt gtagttgagg atgcgggtgt ttagatcgcc atgaaaaaat
     6601 tgatcaataa attgcaatga ttcagctatt ggctttgtaa agattgtagc aaaaatataa
     6661 tttgcagcaa tggtcagcac aagcacagca aatcccacga tgagtatatt ttttgttaag
     6721 gctccaataa gtatattgat tgcaccaagt gattttgccg ttcgtatata gtagttatca
     6781 aaatattttg caacatacag catatcagat ttgacagttt tgctatagcg gattgcactt
     6841 cctacccctt ttgtacggga ttgcagtatt tccgggcggt aaaggtgatt ttccatcaac
     6901 tccggattgg caaaagaatc aaaataaaca gttccatcat ccgtaatgat agtaattctc
     6961 aacgaacttt tatatgatac attttttaat gaagcaacaa catcatgatt ttgaaaaaga
     7021 acataattga aaggtttatg ataggaatgt aaatagatat gtatgttttc aatattttgc
     7081 tgcaactccg cttcttgaaa tgcgatgaaa gcagagtgaa tgtggtgatt gatatacatc
     7141 aaaagaaacg aaataaagat taccagggcg acggtgtata ctactacaag ttttgtcttc
     7201 agccttttca ttactgttta ccaaaaaagt tttactcctt caaactttta gcccataacc
     7261 aacgcccgaa taggtcttta tagcatcttt gtatgttccc agttttttac gcaaattcat
     7321 gatgtggaca tctatggttc tgtcaatgac ataaacatta tcaccacgga tgctgtcaat
     7381 aatattgtcg cgactaaaaa tcttaccggg atactgcata aaaagtttta agattaaaaa
     7441 ttcggttttt gtaaggtcaa tattttgatt gtcgatagtt acctgaaatt tatcaggata
     7501 taatgaaatc gcgttatagg ttaatatttc ttttggcgat agttcctgtt tagtttgtga
     7561 acggcgcatg attgctttta ctctggcaat cagctcatga atgctgaagg gtttggtgag
     7621 gtagtcatcg gcacccagtt ccaatcccag caccttatcc agttcggcag attttgctga
     7681 aagaaatagg atgggtatag tggttaattc tcttgatgca cggatgtgtt tgcataaatc
     7741 cagtccatcc ataccgttca tcatgatatc caatataaat aaatcaggtt ttgcaatagc
     7801 aagttgtttc agtacttctt cagcagaagc aaaggtattg acatcatagc cgtttttttg
     7861 cagattcacc ttcaaaattt cacggatatc tttttcatca tccacaacat atatttttga
     7921 catcgtttac tcctggtgtc gtatgtcctc gccctgtata tagtataccg cacgctcggc
     7981 aatattggtt gcatggtcgc caattcgctc taatgctcgt gcaaccgtga taagcccaaa
     8041 tgctggcgat atggttgcag gattttctac cataaagcta aacagctcac ggttgatctg
     8101 catattaagc tcgtcaacct ctttatccat atctatacat cgctttgcat aatcaatatt
     8161 cttttcggtg attgctctaa acgccagttt tatcatatcg atgcaaattt tttccatacg
     8221 agggatgtca ataagcggtt taagcagtga tttgccctct aaccgtattg tctcttttgc
     8281 gatgttggtt gcaaaatcac ctattctttc taaatcagtg tttgatttaa atagtgctaa
     8341 aataagccgt aagtctgctg ctgcgggctg ctttgttaca aggattctta cacactcttc
     8401 atcaataaaa acctctaatg tatcaagggc agtatctttc tggataactt tttcagctaa
     8461 tttgacatca atatttttta gtgattggac tgaatcttca actgcttcga tggcaatatc
     8521 tgccatctcc agtatcttcc gtattagctc attaagttcc tgctctaaat gagtagacat
     8581 tgttatctcc tttaggtgca ataattaacc aaattttccg gtgatgtaat tttcggtcat
     8641 ctgcttatcg ggtttggtaa acatcacttc ggttttatta acttctatga gtttgcccat
     8701 ataaaaaaat gctgtgtaat cgctaacccg ggctgcctgc tgcatgttat gtgttacgat
     8761 aattatggta tattgctttt ttaaatcctg aataagctct tcaatctttt gtgttgaaat
     8821 tgggtcaagc gctgatgcag gttcatccat cagtattatc tcaggttgca ctgctatagt
     8881 gcgtgcaata cataaccgct gctgctgccc gccggataag ctcattgccg aatcattaag
     8941 cctatcttta acctcatccc acaatgcagt tcgttttaaa gaatcttcaa ctgttttttc
     9001 aatgatatag cgatctttta tgccattaat ctttaatcca tatgcgatgt tatcaaaaat
     9061 agattttggg aatggatttg atttttgaaa taccatgcca acacggcgcc tgagctgagt
     9121 gacatcatat tttatagtat aaatggattc accgtcaatg gtcatgtctc cagcaatgtg
     9181 cgtatacggt attatatcat tcattctgtt aatacaacgt aaaaatgttg atttaccaca
     9241 gcctgaaggg ccaatcaatg ccattaccga attagctaat acagtgaggt ctatatcaaa
     9301 aagcgcctga ctgctaccat agtaaaacga aagatttttt gcttggactt ttatttcttt
     9361 ttcagacata tatcaccgtt attattctta atattaataa taacccatct tattataaat
     9421 aatatattat gaaattgtaa agaaaatgta aagatttata actgttaatt ccaaaacgga
     9481 aaattatgta catataataa taaatgtaca ccccggcgcc gaagacggcg gggtgtacct
     9541 catttcaatt taaaattatt tttaagtaat aaaataaata ctgacaattt ataataaagt
     9601 catgtataaa agcatatatt ttttttaaaa ataaagacgt tattcttcta tagtagccct
     9661 tctggattgt gattggaaga tgtttatcca ttttcattag cataataggg tttctaaaat
     9721 agtaattatt catacaattg tatcagaaaa tagttgccaa ccattttttt caatgattaa
     9781 tcacatgaaa aggtggtttt ttacgatttg tcgataatac ataaaaaaac ataaaattaa
     9841 ttgattcttc ataaaaattt aacaatgcat gactatttat acaaccaatg gagattgcaa
     9901 aatcttctca ttaatagttt acagggaggc ttttatgaag gtcaagagtt ttttggtgtt
     9961 aatgataaca gcatttgtct tctcagtatc aatgggattt tctaaaggca atgatgctaa
    10021 agttgttatc aaaggttcaa caacagtatt accaattgca cagaaggcag ttgaggtgtt
    10081 ttatgacaaa acaaagatta ttatcagtct ttcaggtacg ggttcaggtg atggtataaa
    10141 gtcgctgatt gatggaagct gtgatatagc taattcatca cgtgaaatgc atgaagtaga
    10201 attggagatg gctaaacgaa aaggtaaaac aataaaggaa gtggcaatag cttatgatat
    10261 gattgttccc attgtgcatc cgtccaatcc ggtaaagaat ctttcactgg aacagctaaa
    10321 ggctatctat gaaggaacta tcaccaactg gaaagaggtt ggcggaaaca atgaaaagat
    10381 tgtggtagtg tcccgtgatt caagctcggg aacatttgaa gtatgggaat caaaggtaat
    10441 gaaaaaagct gaagtgcgga aggatgcgtt acttcaagcg tcaaatggag cggttttatc
    10501 ggttgtcgca aggaataaaa aagccattgg ctatgttggc tatggatatc ttgataagac
    10561 cgtgaagggt atttatgtaa acaatgttga gccaaccatc ccaaatggca aatcaggtaa
    10621 gtaccccatc gctcgtaaac tgtacatgta tgtaaatgat gtaaaaatta aacctgaggc
    10681 aaagcagttt attaatttta tcctttcaaa tgaagggcaa ataatagtga aagaagctgg
    10741 gtttattcca attattgatt gatagcttta caataattgg tattatgctg taaacgctat
    10801 attgatgcat attgatatag cgttttttgt ttaaaaaata tttgcaaagt aattgaattg
    10861 ttattatagt aatctattat gtgtagttaa aatagtgttt ggacaactaa gtcaatagaa
    10921 tttggcagaa tggttagaag atgtctgtga tgcattgacn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnt atggatatct taatgataca gtaaaagttt tgtttgtaaa
    11101 tagcgttgag ccaaccatcc caaatgggaa atcaggtaag taccccatcg ctcgtaaact
    11161 gtatatgtat gtaaatgatg taaaaattaa acctgcagct caaaacttta ttgattttat
    11221 tctttcccct gaagggcagt ctatagtaaa agaaacaggg ttcataccaa ttattgattg
    11281 atagttttac aataattggt attatgctgt aaacgctcta ttaatgacta taaatagagc
    11341 gttttctatt aaaaaaaata tttgtaaagt aattggattg ttattatggt acaacttgtt
    11401 ttccttaatt atttcttaaa attcttgtac gttttagctt gactttttag aaatccagct
    11461 tagagcattt ctattgttct atcaaagaat agtcaatttt attaatcaga aacagtcatg
    11521 agcaaaggag gatgcaatga ttttacctat aatcattaat agatggcaca tacttatggt
    11581 tgtggtgatg ataacatata tagccattct ttttgtttat gaagatactg ctgtaagtca
    11641 ggaaaaagcc gttaagcaga tatacctatc atcaaatttg aagaaaaaag gtctggttga
    11701 ggtaagagag ggtattgcct ttgtgaagat acctgcggga agtttcatgc agggtggtaa
    11761 taaagtaaaa gtgaatggat tctggatggc tatgtgtgag gttacgcagc agcaatatga
    11821 atcaattatg ggaataaatc caagttattt caaagggaat cctgataatc cggttgaacg
    11881 ggtaaactgg tacaatgctg ttgagttttg caataagcta agcattaaag caggatgtaa
    11941 accgtattat actattgata aggttcacaa agaccctgag aataagaata ctggtgatac
    12001 aataaaatgg acagtaacaa ttaatggagg gaacggcttt cggttgccgg agagtgcaga
    12061 gtgggaatat gcctgtcgtg cgggcacaac aacaaggtat tactggggtg acaatgtaaa
    12121 tggtgattat tgctggtata atgctaattc agacgaacag acgcatcctg taggcactaa
    12181 attgccaaat gcatggggat tatatgatat gagcgggaat gtatgggagt ggtgttttga
    12241 ctgggacatt gatgattatg gggtattccg gattattcgc ggtggcagct ggaactatga
    12301 tgcagacctt atggagtcca gtgtttgtaa ctatggctat ccaaatgata cgtttcgcat
    12361 cttaggcttc cgccccgtga ggaatttgta aatgccaatt atttttatta cgtacctgcc
    12421 atgatgtata cattttatta tcaataagat tatacttatc ataagaaaat ctttcaagtt
    12481 gtgagatgag ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntctaat
    12601 gagaaaacta tcagaaactt ccgatgacca tggatgtgta ctataaattt ttttcttgcc
    12661 tgcaggtaaa aatatgataa cagtgttatt tataattatt ttatcacaga ggttgttatg
    12721 aagaatatat gtacaattca ggcaggtgca aaggcatacg ccattattaa agatgaagga
    12781 atacaaaaag aaagaattag gtgtatgctt ggtgctgcag gtggtcccaa atggattata
    12841 ctgtacgggc ttgataaagc actgtataaa tcatttttac aaaaaaggaa aaatcctgtg
    12901 tacatgattg gctcatccat tgcaacatgg cgttttgctg cattgtcgct tgctgatgga
    12961 ttgcagggga ttgaagcatt taaagaacgc tactttgcac aaaaatattc aatgaaacct
    13021 gaccgtgagg agataactgc cgaaacggta gcaattcttg aagaattttt gcccgatagc
    13081 aaggtggatg agattgtaaa gcatcccaca caacgtcttt gtattatcac tgcgcgctca
    13141 cggcatcttt tgcagtatga ggatacattc cgtctgggtg ctggattggg aattaatgca
    13201 ataaccaata tggtgagcag aaagctggtt ggattgcatt ttgataggtt tgccttttat
    13261 gatgctcgtg aatttccatc atttatacag tggaaaaact atcatacgca gtacataccg
    13321 ttgaacaaca acaaccttaa aaaagctgta ttggcatccg gctccatccc tcttgttatg
    13381 aacggcgttg tgtttcagca tgagggaaaa acgcatatac tacgggatgg cggtcttgtt
    13441 gattatcagc aggacctgcc gcttgaaccg ggtgactata ttgccctgta tcctcattac
    13501 agtgacagga ttattccggg atggtttgat aaaccactaa agtatagaaa gccgcaccat
    13561 gcattggaaa atgtgtgtat ggtttcaccg tcaaaagaag ctattgcgtc gctgaggtat
    13621 aaaaagatac ctgaccgcaa ggatttctat acctttgcag gtgacgatag caggcgattg
    13681 ttattctgga atgaaacggt tgctttagga cagcagattg ctgaagagtt tatggaactg
    13741 gtggaaagcg gcaggataaa agacagggta attccattag cacgatagtt tgcgtgaggg
    13801 atacgcaagg tgcttgaaaa gcagccacgg gcgtttattt gtagagac
//
LOCUS       DAOSOX010000049        13786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4469_length_13786_cov_8.111791, whole genome shotgun sequence.
ACCESSION   DAOSOX010000049 DAOSOX010000000
VERSION     DAOSOX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_13786_cov_8.111791"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLX22_05280"
     CDS             <1..224
                     /locus_tag="PLX22_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19342.1"
                     /translation="KYTNITDQVIVLESKDDVNRFVDTMPVEKRESIRKLLKYLDSLF
                     DKLPEEVIKNFANSEYFDLYVKVLNELGV"
     gene            230..>2189
                     /locus_tag="PLX22_05285"
     CDS             230..>2189
                     /locus_tag="PLX22_05285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT19343.1"
                     /translation="MGLLEKALQYKQEINKKGKETLIDTITGPADTELVLKESNKSES
                     LDFQDDLLTELSREDLKTIEPVEDELFILPEDDKKTLMEEENTLHTVEEKKVSEYSDT
                     VTSINGKDEYLGPEDEPDLDVLQSEKQEDSTIPGSSKSQNNTKFRIVEPTNNIMDESL
                     KDENVKEQTLSEDISSKPDKKFQDFVVLYEIGKEIVKSKSLNELYDTILFSVMGQIGS
                     ASSTLLVLNPDEENSLIVADSRGITIKNKNIKFDTSRGLFKILMQSKGVADIEKYKQQ
                     TQYRDEYYQLIAIDARLFVPLRYNAIVFGALVLGDKITIGDYNEEEIDFIEQVSEFAA
                     IAYEKVKKIEYLESDIQRYLDDKNFWQSIEEYKQSLRKYPQHDYFKITVQNMMKANGI
                     LSFAVFVKNNKTMDFSLLLTEEDDVLLLNESVSIIKKESPFLDYIASLHLPTIIEDFD
                     RNKAIKEVFTESCMRKMTVFWIYPFQISDELLGFVCVFRLSDYQLKDLQFDNFRSLSD
                     VILTHYWSSRLIEYQYTNYIDVIVPVFNRISKELNNASKLSIPLTIVLFTIKNLKRYY
                     IVQGFNKTKELIDKVEDIIHNRLSEGDFTAKIDRHKFLIVLPGKNKKFAVPFANALKN
                     EIVQQFSQKELQLLVTFLMAEYPEDGNDL"
     assembly_gap    2190..2289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2434..3345)
                     /locus_tag="PLX22_05290"
     CDS             complement(2434..3345)
                     /locus_tag="PLX22_05290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19344.1"
                     /translation="MKLTTVYPVMVLCLIWFHNHSYAQYTIPVISELSRNSQSIMQIR
                     KDIRQSLYTIKSNRSVNELPTLSMYKYVVKGSENFWDIIAVTNLDMDTLISINSLSSP
                     SDIKKGISLFIPNMRGIIYQKNENESYESIAVNYRIPLPYILKINKITGYEHKPYIFI
                     PCGTISDLDRSLFLGTAFVSPLTTGKLSSNFGVRNDPIDHIHRFHAGVDIACPVNSPV
                     YASRDGIVIFAGYKGGYGNLVIIQHSRGYFTLYGHLNTIAVTKGNKVATGHVIAKSGN
                     TGRTTGPHLHFEIRKNYKPINPLLLLR"
     assembly_gap    3375..3474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3609..4382
                     /locus_tag="PLX22_05295"
     CDS             3609..4382
                     /locus_tag="PLX22_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19345.1"
                     /translation="MEEREEKARQIVRSIRALEGVLKTSPDPKQRERVKNDLKALKGK
                     LSELYPDVDLNELIEAIVTDDLVAPVADKSLLEAFEYLKDIEIEKISNFKDDSEINIA
                     ASILKHFSEKIWGVIADQYTKLDYSNSLERDTLYRKLDECQRALKIFQQTVDDIEKAN
                     SSAHISQLNLMRSRQGRLFLIDLFAFFKNVNDFITKILADLEFNGTMVLNADDIITYA
                     KYDVYKTFEGLTVKEALRHLKAFVKEVLIIIKVPDKTKF"
     gene            4455..4877
                     /locus_tag="PLX22_05300"
     CDS             4455..4877
                     /locus_tag="PLX22_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT19346.1"
                     /translation="MALAKSGGDVANNVIGEDSYFSGKFLINGSLRIDGRFEGKYLQA
                     DQLYIGPNGKVKTNINAVSVIIEGLVIGNINATNRVLLMPTAKIYGDIKTPELIIQNG
                     VILEGRCTIANDLKISAKDLIESEYEKNKLIPEEFLAE"
     gene            4867..5847
                     /gene="pdxA"
                     /locus_tag="PLX22_05305"
     CDS             4867..5847
                     /gene="pdxA"
                     /locus_tag="PLX22_05305"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOT19347.1"
                     /translation="MQNKVPVIAITIGDPAGIGPEVTLKALSNFSALLFVPLIICRTE
                     VLIKHYHDIMLPWDSIALNDLQRIHTIDKPCYVTVDFDGPVPNPSNGNELTGKESLCY
                     IDAAIDLWKKNIVDAMVTGPVNKSLIEKWVPFIGHTEYIAQRIHEPNPRMLMYSGRYN
                     VLLVTTHVPVYTLSYHINYESILTTVEICNKYLKKILHKQPVLAICGLDPHCGDKGAI
                     GAFDTDVTAKVVKDAVNNGILIEGPFSADTLFMPQRWQTYDCVIAFYHDQGLIPFKMV
                     AFDEGVNITAGLSIVRTSVDHGTAFDIAGKNCAQFNSMKRAIELACYLSK"
     gene            5861..6304
                     /locus_tag="PLX22_05310"
     CDS             5861..6304
                     /locus_tag="PLX22_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19348.1"
                     /translation="MNTTMLSTSMMMFSVIWGLSVVLLWMRPRIEILWKIAASGIYGF
                     YIWFFYKDLLKGFKQFQKDWYMVTIAFIKEFSGLLFANMFFLWPLALIIIFYKADDVG
                     AERLLKFMCIFTLLVWIVFTLYTFYSKQIDTFLFETLRQMIPDAQ"
     gene            6294..6782
                     /locus_tag="PLX22_05315"
     CDS             6294..6782
                     /locus_tag="PLX22_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19349.1"
                     /translation="MLNKIIITLLLLLQSDDSKFSSSLCNEAKMYAKNNNYNEAGELF
                     LKAASINPYYFYAHYGYGKVCLYTGDIKNAAKHLSIAVTLDASSANGWFYLGFAYFFS
                     KHYMNAVSCFKKAYTLDSSYIESLYNIGVIYEMMGNSFLSQNYYKKYYFAKNKKDTGI
                     LF"
     gene            complement(6774..7109)
                     /locus_tag="PLX22_05320"
     CDS             complement(6774..7109)
                     /locus_tag="PLX22_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19350.1"
                     /translation="MFIDTIKYDSILVITPHEDIIRENSEDIELAVKNNYNKSITDII
                     LDCNHVQYINSFSLGTLVKIHQNLQENNCKLHFLNVAPSIKTLFKITHVLDYFSILNS
                     IDEVKEKSK"
     gene            complement(7175..8212)
                     /locus_tag="PLX22_05325"
     CDS             complement(7175..8212)
                     /locus_tag="PLX22_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19351.1"
                     /translation="MKIKYLILLLPIIMIFSTVTFSQQATEENVIQPDVQSPVSPEVK
                     KEEIVTAALKAVSFNNGVNTFVNSKVKFELIAKDNIATDKILYKLDAGNELEYKEPFT
                     IYDEGKHTISYYSIDKVGNKEDMKTFKVIVDNTPPVVNVAVSAPVIKSGDKLYASKNF
                     TFTIDAKDALSGVQSLEYSFDKTNYQPYLTSFTIPVEGAITLSIKAMDNVANYTEKFV
                     MTAKDENGNPIDLTNGNVAVYVDNKAPLVEIRADKEFLEKDGKKIATVDYNYTVSASD
                     TESGLSSILVRMDGKGEFKAYTGTIKFDTNGEHMIEAKAIDKVGNISSTAVLSVFVDV
                     IAPETLIKSVE"
     gene            complement(8311..8721)
                     /locus_tag="PLX22_05330"
     CDS             complement(8311..8721)
                     /locus_tag="PLX22_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19352.1"
                     /translation="MIKSIYIIALLFLQPTSDFSITLSYHPQPFYHQEWFITLARSQS
                     KAYIATNNPQYLRKKRTITINEYATIVQQLNSLGIWHCKDRYIQGNYYMLTITNRNYR
                     QQCKIEYSPSIPGNNNYSQIIRIILNTANNYLNN"
     gene            complement(8731..9462)
                     /locus_tag="PLX22_05335"
     CDS             complement(8731..9462)
                     /locus_tag="PLX22_05335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT19353.1"
                     /translation="MYNKKRFDIFSSQYKPDIISIEILFAAIDSFADYVYPLFAGNNK
                     ITIIMFDSYYMKKWQVESRDTVSRQTVFSVEKLLCYHPQKKIKHDFYIVNNPDWVNIV
                     AVDKDGNVILVKQHRLGTDEYTIETVAGLIDPGEDPLQAASRELLEETGYKAETFILL
                     KKLAANPAIMNNYIYFYCALDVHKVASQALDIAEDIEIVTLQPGEVISSMKNGTINHS
                     IIVTALLLFFMSPYCSYPKEDIVIY"
     gene            9437..12319
                     /locus_tag="PLX22_05340"
     CDS             9437..12319
                     /locus_tag="PLX22_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19354.1"
                     /translation="MSKRFLLYIAIMMLCLTNRGVFGAGMFHPCYQWYSIKTQHFWIH
                     YHDGLQKQALHLAPIAEAVHETLTREIGLEPFFRTDVVLCDTTDMANGFSMPFPYNRI
                     QLFIVLPHVEDAGLSNNDDWLRLVFTHEYTHILTVDMIGGLPEATRYTLGRACFPNLL
                     MPLWAIEGYAVEQESKEKPWGRLHATYTMMVLRQEVYANNVKDISLASHFPREWPAGM
                     VPYLYGGLFADYVSRNYQTNDFSALFKENADNVLPFLMEKNFNDVYNTGAIVLWKKWQ
                     EELHTAVHNAVDSITKRGLSSYERITSTGYHTSMPRYYGNDIVFATVTGKQKPALMLY
                     STATKQCKKLASVNDPQSISVAGKSIIVSDIEYYQSFNLFRDVFVYDTNYRQKTKGLR
                     VQYIEALSNGYCAIVYSKGRYSLVRLNASFAINDYCIKDTPVQLAYLRVSPDSSQAVF
                     SIVNKGCSNIALYDFKTRGVVLLTDDRYTDIHPAFHPDGKRIVFSSDRDGVFNLYEIN
                     LSNKKISRITNVIGGAFFPDISSDGNTIVFSSYEANGYNIALMPYTTTLSGELSPKPF
                     TVPEEQLTDEHIGNPYNPLHSLIAPWWYPVWYTEELYTDKYDNHAGFLTSGNDTLYKH
                     SYMLEIDYALTQQQMEVKADYIYSGFYPDMFVSYKDDSIGFDDTFPWDPPSNVHYLRR
                     ESEQKITSGFAVPFIKFYNYNQLLLYYTYEKTRIDEYYYTAGTQRYREVLAGGHVALI
                     HDGTYVGSYSISPEDGMIVELYGDMYHTSVASDISYAKVRSGLYYFLRCFANDVLAFM
                     LRGGYAHNAPKYLYPYTIGRYEKGKRHSVPNDEDAYSMRGFTKDEYYGNRLAVAIVEY
                     RIPLVQKDMGYKLVPLLFRDLWLTPFAEYGNIWVGETDIHDFNYSFGSELHLRITAGY
                     HVDIEGFLGVVKGYGDYGETQVYFGVATVFEGALKQHTIIRDAIYN"
     gene            12338..13246
                     /locus_tag="PLX22_05345"
     CDS             12338..13246
                     /locus_tag="PLX22_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOT19355.1"
                     /translation="MQQNLYQALNQGLILERYTCIQRNIEKNKVLIAKWGGLQKVVSL
                     LSGKHVVVCGAGKSLDDATKVLKKYQHRNDLIIIAADMAYRPLIVNGINPHFTISCEA
                     MPVPYYAFLPTSSCHLLAFSGVNANTTGIWKGKISFYNWMIYDEPFNLLWKMAGEHLG
                     YVATASIVTTQAISLSLGCPIASLSIVGNDLAFNHSYYAGNTVVGERFLMGYNRFITA
                     NTQDYAMIQKKKMYIVKRRESIYYTDHQFLAARQWLEDLFSKVTIPVYDCSVPGCGEN
                     IVKKMTIAKVMNQLYPEKKPKKKRQS"
     gene            13243..13434
                     /locus_tag="PLX22_05350"
     CDS             13243..13434
                     /locus_tag="PLX22_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOT19356.1"
                     /translation="MIVVHKMNGDEIILNANHIETIESKPDTIITLVNEKKYLVKESK
                     DEVLSLIYDYFHKVLTGKT"
     gene            13528..>13786
                     /locus_tag="PLX22_05355"
     CDS             13528..>13786
                     /locus_tag="PLX22_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758731.1"
                     /note="Homolog of MotA, appears to be involved in motility
                     on surfaces and under different ionic conditions. With
                     MotS (a MotB homolog) forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOT19357.1"
                     /translation="MDIATIIGLIVGFTNIIMGIIFAKGNPLLFFDIPSVFITIGGSF
                     CALLISNPLSRTLGLWKVAKHAFFPPKYSPNELIITLVSFSE"
BASE COUNT         4385 a         2168 c         2641 g         4392 t
ORIGIN      
        1 aaaaatatac aaatattacc gatcaggtta tagttcttga aagtaaggat gatgtcaata
       61 gatttgttga tactatgcca gttgagaagc gcgaaagtat ccgtaaacta cttaaatatc
      121 tggattcatt atttgataaa ttacctgaag aagttataaa aaattttgcc aattcagaat
      181 actttgatct gtatgtaaaa gtgttaaacg aattaggagt ataaaaagta tgggattgtt
      241 ggaaaaagca cttcagtata aacaagaaat taataaaaaa ggcaaagaga cacttattga
      301 tacaatcaca ggccctgcag atacagagct tgtacttaaa gaaagcaata aatcagaatc
      361 tttagatttt caagatgatt tgctcaccga attaagcagg gaagacttga aaaccattga
      421 gcctgttgaa gatgaattat ttattttacc agaagatgat aaaaaaactt tgatggaaga
      481 agagaataca ttacatacag ttgaagaaaa aaaggtaagt gaatattcag atactgttac
      541 aagtattaat ggaaaagatg aataccttgg gccggaagat gaacctgatc tggatgtatt
      601 acaaagtgag aaacaggaag actcaactat accaggaagc agcaaatccc aaaataatac
      661 taaattccga atagtggaac caactaataa tatcatggat gaatcattaa aagatgaaaa
      721 cgtgaaagag caaacgcttt ctgaagacat atcaagcaag cctgataaaa aatttcagga
      781 ttttgttgtt ttatatgaaa ttggcaaaga aattgtaaaa tcaaaatcgc tgaatgaact
      841 gtatgataca atactttttt cggtgatggg gcagattggt tcagcttcat ctacgttatt
      901 ggtattgaat cctgatgaag aaaattctct tatagtagca gattcacgtg gtattactat
      961 taagaataaa aatattaaat ttgatacatc cagaggtcta tttaagatat taatgcaatc
     1021 aaaaggtgtc gctgatattg aaaagtataa acagcaaaca caatatcggg atgaatatta
     1081 tcagcttata gcgattgatg cccgtttatt tgtaccacta cggtataatg caattgtttt
     1141 tggtgctctg gtgcttggtg ataaaataac aattggtgat tataatgagg aagagataga
     1201 ttttattgaa caggtaagtg agtttgcagc gattgcttac gaaaaagtta aaaaaattga
     1261 gtatttagaa agtgatatac aaaggtatct ggatgataaa aatttctggc aatcgataga
     1321 agagtataaa cagagtttac gcaaatatcc tcagcatgat tattttaaaa taactgtaca
     1381 gaacatgatg aaagcaaatg gaattttaag ctttgctgtt tttgttaaaa ataataaaac
     1441 tatggatttc tcattacttt taactgaaga ggatgatgtt cttttattaa atgaatcagt
     1501 tagtataata aaaaaagaat ctccattttt agattatatt gcgtcattac acctgcccac
     1561 cattattgaa gattttgata gaaataaagc tattaaagaa gtttttacag agagctgtat
     1621 gcgaaagatg acggtattct ggatatatcc tttccagata agtgatgaac ttttaggctt
     1681 tgtttgtgtt ttccggttga gtgattatca attgaaagat ttgcaatttg ataatttcag
     1741 gtcgctgtca gatgttattt taactcatta ttggtcgtcg cggctcattg aatatcaata
     1801 caccaattat atcgatgtga ttgtgccggt gtttaataga atatcaaaag agttaaacaa
     1861 tgcttcaaaa ttgtcaatac cgcttactat tgttttgttt actataaaaa atttgaagcg
     1921 gtattatatt gtacagggtt ttaataaaac aaaagagctg attgataaag ttgaggatat
     1981 cattcacaat cgtctttcgg aaggtgattt tactgccaag atagatagac ataaattttt
     2041 gattgtatta ccaggtaaaa ataagaagtt tgctgtacca tttgctaatg cattaaagaa
     2101 tgaaattgtt caacagttca gtcaaaaaga attgcagttg ctggttacct ttttaatggc
     2161 ggaataccct gaagatggca atgacctgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnc agttgctggt taccttttta atggcggaat accctgaaga tggcaatgac
     2341 ctgtattcac ttcttgatgc aatagattga ttatgagtaa ctatcgcaca aataataatg
     2401 atttttgttt ttaactattt gcataaaggc ttactaccgc agtaataaca aagggtttat
     2461 tggcttatag ttttttctga tttcaaagtg tagatgcggt ccggtggttc tgcccgtatt
     2521 gcctgatttt gctattacat ggccggtagc aactttattt ccttttgtta ctgcaatagt
     2581 atttaagtgt ccatacagag taaaataacc tcttgaatgc tgtataatca ctaaattccc
     2641 atacccgcct ttgtacccgg caaaaataac tattccatcg cgtgatgcat aaactggtga
     2701 attgacaggg caggcaatat caacacccgc atggaacctg tgtatatgat caattggatc
     2761 atttcgtaca ccaaaatttg aacttagttt tcctgttgtt aggggagaaa cgaaagcagt
     2821 tcccaaaaat aaagaacgat ccagatcaga aattgttccg catggtatga agatgtaggg
     2881 tttatgttca taaccagtta ttttatttat ttttaagata tatggaagcg gtatgcgata
     2941 gtttactgca attgattcat agctttcatt ttcatttttc tgataaataa taccacgcat
     3001 attgggtata aacagggata ttcctttttt aatatctgaa ggtgatgaaa gtgaattgat
     3061 agatatcaat gtatccatat ctaaattggt tacagcaata atgtcccaga agttttcact
     3121 gccttttaca acgtacttat acatggaaag agtgggtaat tcattaacgc ttcgattact
     3181 tttgatagta tataaggatt gacggatatc ctttcttatt tgcattatac tctgactatt
     3241 tcgtgaaagc tcactgatga caggtatagt atactgggca taactatgat tatgaaacca
     3301 gataagacat aataccatga caggatatac agtagtaagc ttcatatagt tacatcatat
     3361 aatataaaaa ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcatat
     3481 actactttat aataattgtt aactatgata tgctttgtat tgactggatt ttgaaattat
     3541 gttgcaaaaa aaagataagt ttactatgat agtataaaat tctttgaata ttatatagaa
     3601 aaatatttat ggaagaaaga gaagaaaaag ccaggcagat agttcgttct atacgagccc
     3661 ttgagggtgt attaaaaaca tcccctgacc ctaaacagcg tgagcgagta aaaaatgatt
     3721 taaaggcact taaagggaag ctttctgaat tatatcccga tgttgattta aatgaattga
     3781 ttgaagctat agtgaccgat gaccttgttg ctcccgttgc ggataaatcc ctactggaag
     3841 cctttgagta tttaaaagat attgagatag agaaaatatc aaattttaaa gatgacagtg
     3901 agattaatat tgctgccagt attttaaaac atttttcaga aaaaatatgg ggagtaatag
     3961 ctgatcaata tacaaaatta gactattcaa attctctgga acgtgatact ttatacagaa
     4021 aattggatga atgccagagg gcattaaaaa tatttcaaca aacagttgat gatattgaaa
     4081 aagcaaacag cagtgcacat atttctcaat taaatttaat gcgttctcgt cagggaaggc
     4141 tctttttaat cgatcttttt gcctttttta aaaatgttaa tgattttata accaaaatac
     4201 tggctgatct ggaatttaac ggcaccatgg ttcttaatgc tgatgatata ataacatatg
     4261 ctaaatatga tgtatataaa acatttgaag gtttaacggt aaaggaagct ttgcgccatt
     4321 taaaagcttt tgttaaggaa gttttgataa tcattaaagt gcctgataaa acaaaattct
     4381 aagtaaaatt tcatcttgac attaatataa ttattattta ccagtgataa aaaaattgaa
     4441 gggtaggtgc attcatggct ttagcaaaat caggtggtga tgttgcaaat aatgttattg
     4501 gtgaagattc atactttagc gggaaatttt taatcaatgg atcattgcgt atagatgggc
     4561 gttttgaagg caagtattta caggctgatc agctctatat aggtccaaat ggtaaggtaa
     4621 aaaccaatat taacgcagta tcggtcatta ttgaggggct tgttattggc aatatcaatg
     4681 ctacaaacag agtactattg atgcctaccg caaaaattta tggtgatatc aaaactcccg
     4741 agcttatcat acaaaacggt gtgattctgg aagggcgttg cacaatagct aatgatctta
     4801 aaatttctgc aaaagatctt attgaaagtg aatatgaaaa aaataaatta atcccagagg
     4861 agtttcttgc agaataaagt acctgtaata gcaatcacta ttggtgatcc ggcaggtatt
     4921 ggaccggaag ttacgttaaa agctctaagc aacttttctg cattattgtt tgttcccctg
     4981 attatatgca gaacggaagt gcttataaaa cattatcatg atataatgct tccatgggat
     5041 agcatagcac tcaatgactt gcaaagaata cacactatag ataaaccctg ttatgttacg
     5101 gttgattttg atggaccggt acctaatcct tcaaatggta atgaactgac aggtaaagaa
     5161 agtttatgct atattgatgc ggctattgat ttatggaaaa agaatatagt cgatgctatg
     5221 gtaaccggcc cggttaataa atcgctgata gaaaaatggg tgccatttat tgggcatacc
     5281 gaatatattg cgcagcgcat ccatgaacct aatccaagaa tgctcatgta ttcaggaaga
     5341 tacaatgtgc ttcttgttac aacccatgtg ccggtatata cgctgtcata tcatatcaac
     5401 tatgaatcaa ttttaacaac agtagaaatt tgcaataagt atctcaaaaa gatattgcat
     5461 aagcaaccgg tgctggctat atgcgggctt gatccacatt gtggggataa aggtgctatt
     5521 ggtgcctttg acaccgatgt aacagcaaag gtagtaaagg atgccgtaaa taacggtatt
     5581 cttattgaag gaccgttttc tgccgataca ttatttatgc cacaacgatg gcaaacctac
     5641 gattgtgtta tagctttcta tcatgaccag ggactaatac cattcaagat ggtagctttt
     5701 gatgaaggtg tgaatataac agcaggacta tctatagtaa ggacctctgt tgatcatgga
     5761 acagcatttg atattgcagg aaaaaattgt gctcagttta atagtatgaa acgggcaatt
     5821 gaattagcct gctatctatc aaaatgagga tttgtttagc atgaatacaa caatgctttc
     5881 aacatctatg atgatgttct cagttatatg gggattatct gtagtgctat tgtggatgcg
     5941 tccacggata gaaatactat ggaagatagc agcatcaggg atatatgggt tttatatatg
     6001 gtttttttat aaagaccttt tgaagggatt taaacaattt caaaaagatt ggtatatggt
     6061 aactatcgcc tttataaaag aattcagcgg gctattattt gcaaatatgt ttttcctctg
     6121 gcctctggca cttatcatta tcttttataa ggctgatgat gtgggtgctg aacgcctgct
     6181 gaaatttatg tgcatattca cattgctggt atggatagta tttacattgt atacttttta
     6241 tagcaaacag atagatacat ttttgtttga aacacttcgc cagatgattc ctgatgctca
     6301 ataaaataat aataacatta ttattgctac tacaatctga tgattcaaaa ttttcctctt
     6361 cgttatgtaa cgaagctaaa atgtatgcaa aaaacaacaa ttataacgaa gcaggtgaac
     6421 ttttcctgaa agctgcttca ataaatcctt attattttta tgcacactat ggttatggga
     6481 aggtatgtct ttatactggt gatataaaaa atgctgccaa acatttatct atcgcagtaa
     6541 cgcttgatgc ttcatcggct aacgggtggt tttatttagg ctttgcatac ttttttagca
     6601 aacactatat gaatgcagtc agttgtttta agaaagcata tacccttgat tcatcatata
     6661 ttgagtcatt atataatata ggtgttatct atgaaatgat ggggaattcg tttctttcac
     6721 aaaactacta taaaaaatat tattttgcaa aaaataaaaa ggataccggg atattatttt
     6781 gatttttctt ttacttcatc aattgagttc aggattgaaa aataatcaag cacatgggtt
     6841 atcttgaaaa gcgttttaat ggaaggagca acattaagaa aatgtagttt gcaattattt
     6901 tcctgcaagt tctggtgaat ctttaccaga gtacctaatg aaaagctgtt tatatattga
     6961 acatgattac aatcaagtat tatatcggtt atacttttgt tataattatt tttaacagcc
     7021 aattctatat cttcagaatt ttctcgtata atatcttcat gaggagttat aacaagtatt
     7081 gaatcatatt ttatagtatc aataaacata gttcagtgcc tgaatataaa tgcaacacca
     7141 cagtgctgtg caccatggtg ttgctgtaat catattattc aacagattta attaatgttt
     7201 ctggtgcaat gacatcaaca aatactgaca atactgcagt tgaagatata ttgcctactt
     7261 tatcaatggc ttttgcttcg atcatgtgtt cgccgttggt atcaaactta attgttcctg
     7321 tataagcttt aaattcgccc ttaccgtcca tacgcacaag tattgaagaa agtcccgatt
     7381 ctgtatcgct tgcagaaaca gtgtagttgt aatcaactgt tgcaatcttt ttgccgtctt
     7441 tttctaaaaa ttctttatca gcacgtatct ctaccagagg tgctttgtta tcaacgtata
     7501 ctgcaacatt gccattggtt aaatcaatgg gattgccatt ttcatccttt gcagtcatga
     7561 caaatttctc agtatagttg gcaacattat ccatagcttt gatactcagc gtaatagcgc
     7621 cttcaaccgg aatggtaaat gaagtaaggt aaggctgata gttggtttta tcaaagctat
     7681 attccagtga ctgtacacct gatagagcat cttttgcatc aatagtgaag gtaaagtttt
     7741 tagatgcata cagcttatca cccgatttaa taactggtgc agaaacagct acattaacta
     7801 ccggtggtgt gttgtcaaca atcactttaa aagttttcat gtcttcctta ttgccaacct
     7861 tatcgatgct gtaatatgaa atggtgtgtt tcccttcatc gtaaatggta aaaggctctt
     7921 tgtattcaag ctcattgccg gcatctaatt tatacaatat cttatcagtg gcaatattgt
     7981 ctttggcaat cagttcaaat ttaacctttg aatttacaaa ggtgttaacg ccattattaa
     8041 acgatacagc tttaagggct gccgtaacta tttcttcctt ttttacttca ggagaaacag
     8101 ggctctgtac atcaggctgt ataacatttt cttcagttgc ctgttgagaa aatgttaccg
     8161 ttgaaaagat catgataatg ggtaatagca atatgagata tttaatcttc atataacctc
     8221 ctttttggtt aataatctta ttgtgttttt acgaatgtcg taaagataaa taaatctatc
     8281 catttgcaca atagtttgtc aaatgaattt ttagttattt aaataattat ttgcagtgtt
     8341 cagtattata cgaattatct gtgaatagtt attattgcca ggtattgaag gggaatattc
     8401 tattttgcat tgttggcgat agttcctgtt agtaattgtc agcatatagt aattcccctg
     8461 tatataccta tccttgcaat gccatattcc cagcgagttc aattgctgaa cgatggtagc
     8521 atactcattg atggtgatcg ttcttttttt ccgtaaatat tgggggttat tggttgcaat
     8581 gtaagctttt gattgtgaac gagccagtgt tataaaccat tcttgatgat aaaacggttg
     8641 tggatggtac gacagcgtta ttgaaaaatc agatgtgggt tgcaaaaata ataatgctat
     8701 gatgtaaata cttttaatca ttgccaattc ttaataaatg actatatctt cctttgggta
     8761 agagcaatag ggcgacataa aaaaaagaag cagtgctgta acaatgattg aatgattgat
     8821 agtgccattt ttcatactgc tgattacctc acccggttgt aatgttacta tttcaatatc
     8881 ctctgctata tcaagtgcct ggctggcaac cttgtgtaca tccagtgcac aataaaaata
     8941 gatatagttg ttcataatag caggattagc agccagtttt tttaaaagta taaatgtttc
     9001 agctttatag cctgtctctt ccagcaattc acggcttgct gcctgcaatg gatcttcgcc
     9061 cgggtcaata agacctgcaa cagtttcaat ggtgtattca tccgtgccaa gcctgtgctg
     9121 ttttaccagt attacattgc cgtctttgtc aacagcaaca atatttaccc agtcaggatt
     9181 gtttacaata taaaaatcgt gctttatttt tttttgggga tgatagcata aaagcttttc
     9241 aacagaaaaa actgtctgcc tgcttactgt atctctactt tctacctgcc actttttcat
     9301 ataataacta tcaaacatta taattgtaat tttgttattg ccagcaaata aaggatatac
     9361 ataatcagca aaactatcta ttgctgcaaa taaaatttca atagaaatta tatcaggctt
     9421 atactgtgag ctaaaaatgt caaaacgttt tttattatac attgctatta tgatgctatg
     9481 tttaactaac cgtggggtgt ttggtgcagg gatgtttcat ccttgctatc aatggtattc
     9541 aatcaaaacg caacatttct ggatacatta ccatgatgga ttgcaaaagc aggcgttgca
     9601 ccttgcacca atagccgaag cagtccatga aaccttaacc agagaaatag gcttggagcc
     9661 tttttttaga acagatgtgg tgctatgcga cactaccgat atggcaaacg ggttttccat
     9721 gccgtttcct tataacagga tacaactttt tattgtcctt cctcatgttg aagatgcagg
     9781 gctatcaaat aatgatgatt ggttacgcct tgtctttacc catgaatata cgcatatcct
     9841 gaccgtggat atgataggcg gattgcctga ggctacacgc tatacgcttg gaagggcatg
     9901 ctttcccaac ctgttgatgc cgctatgggc tattgaaggc tatgctgttg aacaggaatc
     9961 aaaggaaaag ccatggggaa gattacatgc cacctatacc atgatggtgc tgcgccagga
    10021 ggtttatgct aacaatgtaa aggatatatc acttgcgtca cattttccac gggaatggcc
    10081 tgcggggatg gtgccctatc tttacggtgg gttgtttgcc gattatgttt cacgtaacta
    10141 tcaaacaaat gattttagtg cgctttttaa ggaaaatgct gacaatgtgc tgcccttttt
    10201 aatggaaaag aattttaatg acgtgtataa tacgggggcg atagttctct ggaagaagtg
    10261 gcaggaggaa ttacatactg ccgtacacaa tgctgttgac agcataacaa aaagaggatt
    10321 gagcagctat gaacgtataa ccagtacagg ctatcacacc tccatgccac gctattatgg
    10381 aaatgacatc gtttttgcga cagtaaccgg caaacaaaaa ccagcattga tgctgtacag
    10441 tactgcaaca aaacagtgta aaaaacttgc cagcgtcaat gacccgcaga gcataagtgt
    10501 tgccggaaaa agcattattg tcagtgatat tgaatattac cagtcattca atctttttag
    10561 agatgtcttt gtatatgata caaactatcg gcaaaaaaca aagggtttgc gtgttcaata
    10621 tatagaggcg ctttccaatg gatattgtgc aattgtatac agtaagggaa gatattcact
    10681 ggtacggtta aacgcttcgt ttgctatcaa cgattattgt attaaagata caccggtaca
    10741 gcttgcatac cttcgtgtgt cgcctgatag ttcgcaggca gtgttttcca ttgtcaataa
    10801 aggttgcagc aatattgcac tctatgattt taaaacacgg ggggtagtat tgctaacgga
    10861 tgatcggtat actgatattc atcctgcttt tcatcctgat ggcaaacgaa ttgtattttc
    10921 ttcggacagg gatggtgtgt ttaatctata cgagataaac ctatccaata aaaaaatttc
    10981 ccgtataacc aatgtaatag ggggagcgtt tttcccggat atcagcagtg atggaaatac
    11041 catcgtgttt tcatcttacg aggctaatgg ttataacatt gccctgatgc catacaccac
    11101 aaccttatcc ggagaactat cgccaaaacc ttttaccgtc cctgaagagc agctaacgga
    11161 tgaacacatt ggcaaccctt acaatccctt gcattcgctg atagcgccat ggtggtatcc
    11221 tgtatggtat accgaggagc tatacacaga taaatatgac aatcatgctg ggtttttaac
    11281 atcggggaat gatacactgt ataaacacag ctatatgctg gagattgact atgcattgac
    11341 ccagcaacaa atggaggtta aagccgatta tatctattca ggtttttacc ctgatatgtt
    11401 tgtgtcgtac aaagatgata gcataggttt tgatgatacc tttccatggg atcccccatc
    11461 aaatgtacat tacctgcggc gtgaatctga acaaaagata acatcaggct ttgcagtgcc
    11521 ttttataaag ttttataatt ataatcaatt gcttctttac tatacctatg aaaaaactcg
    11581 cattgatgag tactattata ctgcagggac tcaacgatac agggaagtgc tggcaggtgg
    11641 tcatgttgca ttgatacatg atggaactta tgtcgggagt tattccataa gtcccgaaga
    11701 tggtatgatt gtggaattgt acggggatat gtatcacacc tcggtagctt cggatatttc
    11761 gtatgccaaa gtgcgttcag gactgtacta ctttttacgc tgttttgcca atgatgtgct
    11821 ggcgtttatg ctgagaggcg gttatgccca taatgcgccc aaatatcttt atccctatac
    11881 cataggtcga tatgaaaaag gcaaacgaca cagcgtacca aatgatgagg atgcctacag
    11941 tatgcgtggt tttacaaagg atgagtatta tggtaacagg ctggcagtag ctatagtgga
    12001 ataccgcata ccgttggtac agaaagatat gggttacaag ttggtgccac tgctgttcag
    12061 agatttgtgg ttgacaccgt ttgccgaata tggcaacatc tgggttggtg aaaccgatat
    12121 acatgatttt aactatagct ttgggtcaga gcttcacctg cgcattaccg ccggctacca
    12181 tgttgatatt gaaggatttt tgggagttgt gaaaggctat ggcgattatg gggaaacgca
    12241 ggtatatttt ggtgttgcta ctgtctttga aggagcattg aagcagcata ccattattcg
    12301 ggatgccata tataattaat gcagtaaggt tgtctccatg caacaaaatc tttatcaggc
    12361 acttaatcag gggcttatac ttgaacgcta tacctgtatt cagcgcaata ttgaaaagaa
    12421 taaggtgctc attgcaaagt ggggcggttt acaaaaggtt gtatcgttat tatccggaaa
    12481 acatgttgtt gtttgtggcg caggtaaaag tctggatgat gctacaaagg ttttaaaaaa
    12541 atatcagcat agaaatgatc ttatcattat tgcagcagat atggcgtata ggccacttat
    12601 tgtcaatggt attaatcctc attttactat ctcatgtgaa gcaatgcctg ttccatacta
    12661 tgcgttttta cctacatcat catgccatct tttagccttt agtggtgtca atgccaatac
    12721 cactggtata tggaaaggaa agataagctt ttataactgg atgatatatg atgaaccgtt
    12781 taatctttta tggaagatgg caggggagca tcttggatat gttgcaacag caagcattgt
    12841 gacaactcag gctatatcat tatcgttagg ctgtcccata gcatcgctgt ctattgtggg
    12901 gaatgatctg gcatttaacc atagctatta tgctggcaat actgtggttg gcgaacgctt
    12961 tttaatggga tataatagat ttatcacagc aaatacacaa gattatgcaa tgatacaaaa
    13021 aaagaaaatg tatattgtta aacgccgtga gtcgatatat tatactgatc atcaattttt
    13081 agctgccaga caatggctgg aggatctttt ttcaaaggta acaataccgg tgtatgattg
    13141 cagtgtacca ggttgtggcg aaaatatagt aaaaaagatg acaatagcta aagttatgaa
    13201 tcaactgtat cctgaaaaaa aaccaaagaa aaagaggcaa tcatgatagt agtgcataag
    13261 atgaatggtg atgaaattat tttaaatgcc aaccatattg aaacaataga atcaaagcca
    13321 gatacaataa taacccttgt taatgaaaaa aaatatctgg tgaaggaatc aaaagatgag
    13381 gttttatctt tgatctatga ttattttcat aaagtactta ccgggaaaac gtgatagtat
    13441 tttgatgaat tattttaaaa ttttaaccga aaattatata tacgatatac tgtattatgt
    13501 aaagggctat gaggagggtt gtttcatatg gatatagcta ccatcattgg actaattgtt
    13561 ggttttacaa atattatcat gggaataatc tttgcaaaag gtaatccgtt gctatttttt
    13621 gatatacctt cggtttttat tacgattggc ggttcgttct gtgctctgtt gatctctaat
    13681 cctcttagca gaacattggg attatggaaa gttgcaaagc atgctttctt tcctccaaaa
    13741 tatagtccca atgaactcat cataaccctt gtatcatttt ctgaaa
//
LOCUS       DAOSOX010000050        13572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4559_length_13572_cov_8.264186, whole genome shotgun sequence.
ACCESSION   DAOSOX010000050 DAOSOX010000000
VERSION     DAOSOX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13572
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_13572_cov_8.264186"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1228
                     /locus_tag="PLX22_05360"
     CDS             83..1228
                     /locus_tag="PLX22_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19358.1"
                     /translation="MTPIISSLLSVMLIFTQVPQRSVAGKEYIICGNNTQQKIYYTTS
                     SNYSKNHKGEKAIDNDTVTSWMSQHDGDQWIEIDFVVKRVITDIKVVAGYRDNYQTIT
                     YFILQFMYDGKWFDFARVDFTRKEKKGLFGTHSVYNPVAVAALGGIDASTFRIYIPQD
                     AIVNGYAAIAEIEVYAGSGKLQYFDKRLYNLCFPIKNGLFPENDAGYPNSPRGYRGGI
                     HYGLDILYYFNEDTYQPIPLDETTPVLACADGVVIRADYDYKPPAEEEWKKLSLYYQG
                     NPATFVQRSFGGRQIWIDHQNGVLSTYNHLSKIDNKVSAGSRVKKGQRIGWVGNSGLL
                     GEAQGKKWGQHLHFELWVDGIYLGYNMNINEIKRYLRWIFAIRDMEE"
     gene            1231..2400
                     /locus_tag="PLX22_05365"
     CDS             1231..2400
                     /locus_tag="PLX22_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19359.1"
                     /translation="MTDIKKRAYQFIIVFGIISLLGDIIYEGARSVNAQYLKGLGANA
                     LWVGIVAGLGEFVGYAIRLVSGYLSDKTRAYWLFTFIGYGLLITVPLLALTGIWQVAA
                     LLMIGERLGKALRSPAKDTILSQVTTQVGRGLGFGIHEAMDQIGAIAGPLMFTFIFAY
                     VVDYNLGYKIMWIPFILLMGVLIIAKMLVPHPEQFESTPHSNSSDALTPTFWLYNAFS
                     AFAIIGFASFPIIAFHLKSQNIVSDMAIPLLYAIAMGVDAVFAIIIGRLYDKKGLSVL
                     LVIPIASAIVPVMAFSFHWVAVTIGIILWGLVMAVHETIMRAAIADLTSLKKRGTGYG
                     IFNTAYGLCMFIGATAMGWLYEIDMRYIMAFSFINELIAVVLFVYIIKSIHSQMK"
     gene            2495..2857
                     /locus_tag="PLX22_05370"
     CDS             2495..2857
                     /locus_tag="PLX22_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19360.1"
                     /translation="MALEQKDMEFIEHYLREHLPRILAEQSLAKPPVVYEIELRERMV
                     RVEEELKHQRELMQKGFELMEKSFQMMERRFEQIDKRLELLENRFDNLLKRLFQFMIW
                     TFGMIISATGIIIAFMKM"
     gene            2910..3680
                     /gene="xth"
                     /locus_tag="PLX22_05375"
     CDS             2910..3680
                     /gene="xth"
                     /locus_tag="PLX22_05375"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT19361.1"
                     /translation="MKLKLISWNVNGIRAVYKKGFYDWFINNNADIVCLQETKATEEQ
                     FPEELLKIPHYHKYFSTAEKKGYSGVALFTRIQPVKVEKGFGIQCFDNEGRILIAYYD
                     AFVLYTIYFPNGKASKERLQYKMDFYDAFLEHAENVRKNGHSIIMCGDVNTAHTELDL
                     ARPKENETVSGFLPQERAWIDKFISYGYVDAFRLFVKEGGHYSWWDYKTKARERNIGW
                     RIDYFFISEDLKTKVKDAFIQKDIEGSDHCPVGIVIDL"
     gene            4064..4249
                     /locus_tag="PLX22_05380"
     CDS             4064..4249
                     /locus_tag="PLX22_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT19362.1"
                     /translation="MPVYEFKCKHCNAVFSELRKIGDTKAPECPDCHSDDTEKIFSVF
                     SSGAGVKPSCASSSGGG"
     gene            complement(4449..4697)
                     /locus_tag="PLX22_05385"
     CDS             complement(4449..4697)
                     /locus_tag="PLX22_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19363.1"
                     /translation="MHICLFKNKIILTIAFITSEKDGWVEIFIGHLPDSKWVEDKLVN
                     TGYNDDISLVVDAASYEKALNLLKDTSKPANSKQAKTY"
     gene            4880..5158
                     /locus_tag="PLX22_05390"
     CDS             4880..5158
                     /locus_tag="PLX22_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19364.1"
                     /translation="MKRTIAIVIVFLCIPPLYAEKKNYCELMRELVINISKEAKKHNP
                     EFIIIGQNALELLTINGKPTGKLANNYCHAMDAIGIEELFWGYSLNKR"
     gene            5118..5483
                     /locus_tag="PLX22_05395"
     CDS             5118..5483
                     /locus_tag="PLX22_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOT19365.1"
                     /translation="MKSFSGGIPLTNDDVHRLQKKPNGARRLVIAYMSVGEAEDYRYY
                     WKAGWEKSKPQFLEQENKLWKGNYKVRYWDKQWHVILYGNGNEELFGDSYPGRVIAAG
                     FDGVYMDVLDAAHYFQEKK"
     gene            5587..7626
                     /locus_tag="PLX22_05400"
     CDS             5587..7626
                     /locus_tag="PLX22_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19366.1"
                     /translation="MGSLKGKFKSLKVILSTIVGLIVTTAIIILIIISYNAAYSELEK
                     SYVNQIQNVNSGINMLVEQYFAQNIQVAELYASRDDVRESLVMGNFGPVTKELTAFLN
                     KYKELYENIFIATADSNPVILAAGGPGAVGTRYRNASYARAIDEALQGKVGLSKPDKS
                     PVTGQPVILIQVPIRENGRIVGLLGLPLELGKYSYKLVKDVKIGYTGYPYVTDIDGLV
                     FAHPEKENILKLEMSKLDFGQKLLSSPDNTVIKYKWEGKDKMLVGEKNKGYGFIVAST
                     MYMSDIANSARDMATTMVLFGLIMLAGAVIGIYYIIASRLNPLEKLKTIAGSLAEGNV
                     TMRYEGRVYSDEIGEMSTAINNTMENLEKLVAEVKVAVSNLTQAVNEIASGNENLSQR
                     TSEQASSLEEIAATIEQATATIKQNADHASRANQIAEKTSELAKNGNTLVGDAVVAIN
                     EINASSKRIGEILTLINEISFQTNLLALNAAVEAARAGEQGRGFAVVAGEVRNLAQRS
                     GSAAKEIGELIRDSLDKIDTGTTLVNKSGEALKEIMQSIQDLYKTITEIATASDEQKQ
                     GIDQINVAISDLDTMTQQNAALVEETASASEEMANQAQELADMMERFVIRDEVSQKIY
                     GTKHKELHLHAADSAKKPVTKKVDKQVKSKEEKKEEEKKEEGKNNKLIDDGFEEF"
     gene            7652..9733
                     /locus_tag="PLX22_05405"
     CDS             7652..9733
                     /locus_tag="PLX22_05405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT19367.1"
                     /translation="MAEDAIRQVFLAETKELLENLENDIVRYEETKDPELIHSIFRYV
                     HTLKGGSGMAGYDDVYQFTHALENVLDSVRNGIIPMDDLLADIVFASIDVIRLRLYTD
                     SDQQQMLEKFNLLQSKINDIETLLQKKEEAEPAEEPEEKKFIYNFFRVKAKFRDDIFK
                     FGIDPLIIMEDLSSLGKIVELRVNRNNVPLLQDIDPEQCYIAWDVILKTKSKAEALDD
                     VFIFVRDDNEITITDVTKNYVAEQVIEDVPKLGELLLSRGMLTENEFEDVLNEHEKTK
                     KKVGEIAVTKGYIEENDVSLALKEQDEIKKQISTSTLRVDAKKVDNLMNLLGELVIGF
                     SGIKRIAEEIEDEKAYRLNNALYSVDRIIREFQEHLMRVRMVPIGPIFEQYRRFIRDT
                     AKAHGKSIRLEITGGDTEIDKTVIERISDPLKHLIRNAIDHGIEAPHDREEKGKPREG
                     LVSLKAYHQEGNMYIEVSDDGAGIDKEKVIEKAVAKGLLSKNDEIPDSTLYGFLFEPG
                     FSTSESVGDLSGRGVGLDVVKTNIEALRGTVDILSQPEKGTTFIIKLPLTLAIIDGML
                     VRVAHETFIIPILSIIESIKPVKDDIKTIESQGEVVHIRGEYVPLLHLHQEFNLPQTI
                     QNPWDGLVVVVESQGRSIGLIVDELIGQQQIVIKSLDNQITTSRAISGAAILGDGTIA
                     LIIDVHGFINR"
     gene            9761..11491
                     /locus_tag="PLX22_05410"
     CDS             9761..11491
                     /locus_tag="PLX22_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19368.1"
                     /translation="MMNYPVKEQSFKSQHEYLKNLKSNVGSLFSEIIQQFTTINKRID
                     DFFTMIRPYIIYFYTTDEAIADTFLHDVIFSTKDEINGYIETIVKYMIADRETNREVE
                     EAINKTLTLSNSVEMILDAVEAIDLYAKNTMIIAIKAGEEGTTLTTIAVEMSHLADMV
                     NAVSSEFQEIIEQLHSLRTKFSETCMTVDVIVENYLTQLQVRLNNTINDIIRHQKNLS
                     LNVNSIKNFADQIKAVIQKILMEFQIEDIVRQDIEKILFAIEALEESINTDEKEQDVL
                     LWGIYKKLLSLHNDFVHLLDSTVRGINFTRESIVQINNDYNKNAINVYEGDEFAIEKV
                     YNSLEYLQNETIKYIDDILMRKKYLLDISKDVLVRLEKFQEFFRRIQDVVRKFDIVNM
                     LTRIELARHRELQKTIASSLSDISIMPKKIKMIVEESENLYKDVISTMESTYQLYQQA
                     MSIQDEILKNCVNNLKKVSLELYESKKYYVDISQQIERNIHSLQQVLDEKSEQIMAFH
                     EIGKRLKFLCDSMEDMDILKETPELSTIDKIIESINANYGNDYKGLMLISLLKEYSTL
                     QSEDTVIVFK"
     gene            11526..11780
                     /locus_tag="PLX22_05415"
     CDS             11526..11780
                     /locus_tag="PLX22_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19369.1"
                     /translation="MYIIEIDEFVTIKHIKQFLDEVKTVLMTEKDIVLDFSKAKRLDL
                     SIVQVILSLLKTAKAEGKTVKFRGVNDTIKKQLKLCGVIR"
     gene            11830..12201
                     /locus_tag="PLX22_05420"
     CDS             11830..12201
                     /locus_tag="PLX22_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT19370.1"
                     /translation="MKYIIIIDDSPTIRTSVEFTLKDIGFGLMHAENGIDALNKIQQL
                     SANGDEVAMCIVDINMPQMDGISFIKKFRENDKFAPVIVLTTEAEEEKIQEGKKAGAS
                     GWMIKPFKAEQLQSVVHKFLR"
     gene            12214..12705
                     /locus_tag="PLX22_05425"
     CDS             12214..12705
                     /locus_tag="PLX22_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT19371.1"
                     /translation="MNIENTTVENKLDTTSTEQFVTFIIGNETYGIEVLKVQEIIGMT
                     RITYVPNTLPYMKGVINLRGSVVPVIDMRKKIGMDEIAYNAFTVIIITEVKGKLIGMI
                     VDSVQDVVTIPIQKIQDTIHFTSQIATDYIKGIGQIDNNLVIILDVDRLLTADEIAYI
                     KNI"
     gene            12752..13315
                     /locus_tag="PLX22_05430"
     CDS             12752..13315
                     /locus_tag="PLX22_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19372.1"
                     /translation="MIRTTDATINKEVNILLPGEYYASSDSILSTIVGSCFVVCLYDP
                     LKKIGAMGHCLLPVLSNSQMKKNEIFQYNINFMEHIIGEMVKLGADRKTFVVKVFGGI
                     GKTFNLEYTTDFIKDYCKNENMLLEAIDVGGNYRRKLYYFCKTGKVHKYLVKQNDALS
                     EFITMEKEFIDKVLSDKVQYGNVIIFE"
     gene            13319..>13572
                     /locus_tag="PLX22_05435"
     CDS             13319..>13572
                     /locus_tag="PLX22_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheR"
                     /protein_id="HOT19373.1"
                     /translation="MNNIISYQQLNQRDFDYLRNIIYDEAGIKLSDMKKALVQSRLLR
                     RMREMGISSYDEYCHYLRENYDEEIENLINCITTNKTEFF"
BASE COUNT         4574 a         1957 c         2941 g         4100 t
ORIGIN      
        1 ataaaaaagt gctggatttt agcaaagttg tattagcatg tatgtcattt atttacaatt
       61 ttaaagtaaa gaatgatgat atatgacacc aataatttct tcactacttt cggttatgct
      121 tatttttact caggttcccc aaagaagtgt agcagggaaa gagtatatta tctgcggcaa
      181 taacacccag caaaaaatat attataccac cagcagcaac tatagcaaaa atcataaagg
      241 tgaaaaagca attgacaatg atacggtaac atcgtggatg tcacagcatg atggcgatca
      301 gtggatagaa atagacttcg tcgttaaacg cgtgataact gatataaagg ttgttgcagg
      361 ttatagagat aactatcaaa caattacata cttcattttg cagtttatgt atgatggtaa
      421 atggtttgat tttgccaggg ttgattttac cagaaaagaa aaaaaaggat tatttggcac
      481 acatagtgtc tataatccgg tggcggttgc tgcactgggt ggtatagatg ccagcacatt
      541 cagaatatat atcccacagg atgcgatagt taatggatat gctgccattg ccgagattga
      601 ggtgtatgca ggtagtggaa aactacagta ttttgacaag aggttatata atctgtgttt
      661 ccctattaaa aatgggttgt tccctgaaaa cgatgctggc tatcccaatt cgccacgagg
      721 gtaccgtggc ggcatacatt acggtcttga catattatat tatttcaatg aggataccta
      781 tcagccaatc ccgctggatg aaacaacacc ggttttagca tgtgctgacg gggtggttat
      841 acgagcagat tatgattata aaccaccagc agaagaggaa tggaaaaagc tttcgctgta
      901 ttatcaaggc aatcctgcaa catttgtaca acgatcattt ggtggcagac aaatctggat
      961 agatcaccaa aatggggtat tatctaccta taatcatctt tcaaagattg ataataaagt
     1021 aagtgctggc agcagggtaa aaaaaggtca acgcattgga tgggtgggaa attcaggact
     1081 gttaggcgaa gctcagggca aaaaatgggg gcagcacctt cactttgagc tgtgggtgga
     1141 tggtatttat ttagggtaca atatgaacat taatgaaata aaacgttatt tgcgatggat
     1201 atttgcaatt cgtgatatgg aggaataaca atgaccgata taaaaaagcg tgcataccag
     1261 tttattattg tttttggcat aataagcctt ctgggcgaca ttatctacga aggtgctcgc
     1321 agtgttaatg cacaatattt aaaggggctg ggtgccaatg cgctgtgggt tggcattgtt
     1381 gcaggattgg gggaatttgt aggctatgca atacggcttg tttcaggata cttaagcgat
     1441 aaaacaagag catactggct atttaccttt atagggtatg ggctgctcat cacggttcct
     1501 ttgctggcat taaccggcat atggcaggtt gcagctcttt tgatgattgg tgagcgttta
     1561 ggaaaggcgt tgcgaagccc tgcaaaggat acgattctgt cacaggtaac aactcaggtt
     1621 ggcagagggt tggggtttgg catacatgaa gcaatggatc agattggtgc tatagccggt
     1681 ccgctgatgt ttacgtttat ctttgcctat gtggttgatt acaacctggg gtataaaatc
     1741 atgtggatac catttatttt gcttatggga gttttgatca ttgcaaaaat gctggtacca
     1801 catcctgaac agtttgaaag cacaccgcac agtaattcgt cggatgctct gaccccaaca
     1861 ttctggctat acaatgcgtt ttctgcattt gcaatcatag ggtttgccag tttccccatt
     1921 attgcctttc acttaaaaag tcaaaatatt gtcagcgata tggccatacc tttgctgtat
     1981 gcgatagcaa tgggggttga tgctgtcttt gccataatta ttggcagatt gtatgataaa
     2041 aagggactat cagtactatt agttatacca atagcttcgg cgatagttcc ggtaatggct
     2101 tttagttttc attgggttgc agtgacgatt gggattatac tgtggggact tgtgatggca
     2161 gtacatgaga cgatcatgcg tgcagcgatt gctgatttga catcactgaa aaaacgcggt
     2221 accggctatg gcatattcaa taccgcctat gggctctgca tgtttattgg tgcaacagca
     2281 atgggctggt tgtatgagat tgatatgcgc tacattatgg ctttttcgtt catcaatgaa
     2341 ttgattgccg tggtgctttt tgtatacatt ataaaatcaa tacattcaca gatgaaataa
     2401 tgtattgaca ttactatgat tatgtactac attattatac aaaagcaata tatagtatat
     2461 gtttttgcta attttgtaat taagggatgg taatatggca ttagagcaaa aggacatgga
     2521 gtttatagaa cattatttaa gagaacattt accccgtatc ctggctgagc agagtctggc
     2581 aaaaccgccg gtggtatatg agatagagct tcgtgaacgg atggtgcgtg ttgaagaaga
     2641 gttgaaacat cagcgtgagt tgatgcaaaa gggttttgaa ttgatggaaa aaagttttca
     2701 gatgatggaa agacgatttg agcagataga taaacggtta gaattgctgg aaaatagatt
     2761 tgataatctt ttaaagcgat tattccagtt tatgatatgg acatttggaa tgatcatatc
     2821 agctacagga attattattg catttatgaa gatgtagtgg ttgaggtaat gcactgctgc
     2881 tactgcatat ataaaagaga aaaaacaata tgaaattaaa acttatttcg tggaacgtca
     2941 atgggattag agctgtttat aaaaaagggt tttacgattg gttcatcaac aacaatgcgg
     3001 atattgtatg cttgcaggaa acaaaggcaa cagaagaaca atttcccgaa gagcttttaa
     3061 aaattcctca ttatcacaaa tatttttcaa ctgccgaaaa aaaagggtat agtggtgttg
     3121 ctttatttac caggatacag ccggttaaag ttgaaaaagg atttggcatt caatgttttg
     3181 ataatgaagg acgcatactc atagcgtact atgatgcgtt tgttttgtat actatctatt
     3241 tccccaatgg caaagcatca aaggagcgcc tgcaatataa gatggatttt tacgatgcat
     3301 ttttagaaca tgctgaaaat gtgcgcaaaa atggtcatag cattatcatg tgtggtgatg
     3361 ttaacacggc ccatactgaa cttgatttag cccgtccaaa agaaaatgaa accgtttcag
     3421 gatttttacc acaggaacgt gcatggatcg ataagtttat atcctatggt tatgttgatg
     3481 catttcgttt atttgtaaaa gaaggtgggc actactcatg gtgggattac aaaacaaagg
     3541 ctcgcgagcg taatatagga tggaggattg actatttctt tatcagtgaa gatttaaaaa
     3601 caaaggtaaa agatgcattt atacaaaaag atattgaagg ttcagatcat tgccctgtgg
     3661 gtattgttat cgacctataa tcgatagagc gctttttttt ggtagctcca ttgtcacagc
     3721 ctttccatgc actttgcttg atgctattat aactgcgatt ccatgtttta agcgataatt
     3781 ccattatgca catcctttat cttatacaga attaatatgc tgttaatata aataaaaaat
     3841 gttttagcca atttattgtt actgtaaatt ttaaaaatgc ttaataaaaa atatacaaaa
     3901 caattatggg cgatagttac tgatgaacgg ttttattttc taaaaaattt tattgaaacc
     3961 aattgaatgt tagtatatag taaaatacta tacaacctgt tttgtgttat ggaagctaca
     4021 ttacttatta tttcatttca ttataacagt agaggattat tatatgccag tatatgaatt
     4081 taaatgtaag cactgcaatg ctgttttcag cgaattgcga aagataggtg atacaaaggc
     4141 gcctgaatgt ccagactgcc attcagatga tactgaaaaa atattctcgg tattttcaag
     4201 tggtgccggg gtaaaaccat catgtgcatc atcatcgggc ggtggatgag gtatataatc
     4261 tgagccagtt tggctcttta taaggctctg accccaatgt gtcaataaca gtacttattc
     4321 atgctcaacg tgttgtactg cactatttta agtgtcagct tctatagttt cttttatctt
     4381 ttctactgat tcattaaggg tagcaataga tagttcacca attatatttt gtttttctga
     4441 tagctcattc aatatgtttt agcttgctta ctattggcag gttttgacgt atcttttagt
     4501 aagttaaggg ctttttcata acttgctgca tcaacaacta aactgatatc atcattataa
     4561 cctgtattta ccaacttgtc ttcaacccat ttactatctg gtaaatgacc aataaaaatc
     4621 tcaacccagc catctttttc tgaggtaata aatgctatag ttagtataat tttattttta
     4681 aataagcata tatgcaattg aattgattgt caagaaaaaa cgggtggtga aacgctatgc
     4741 taatcaaacg ctgtaaaaaa gggtcagagg ggtcagagcc tatgtacagt taatcccgta
     4801 aaaaatttct tgcatatatt attgaatcga ttatcatttt attttgcact ttgatgatag
     4861 tgaaagggta tgccactcta tgaaaaggac tattgctatt gtcatagttt ttttatgtat
     4921 ccctcctctt tatgcggaaa agaaaaatta ctgtgagctt atgcgtgaac ttgttattaa
     4981 cattagtaag gaagcaaaaa aacataatcc tgaatttatc atcattggac agaatgcgct
     5041 ggagctgctt accattaatg gtaaaccaac gggcaagctt gcaaacaatt attgtcatgc
     5101 aatggatgca atcggcattg aagagctttt ctgggggtat tcccttaaca aacgatgatg
     5161 ttcacaggtt gcagaaaaaa ccaaacgggg cgcgcaggtt ggtgattgcc tatatgtcgg
     5221 ttggcgaggc ggaagattac cgctattact ggaaagccgg gtgggaaaag tcaaagccgc
     5281 aatttctgga acaggaaaat aagctatgga aaggcaacta taaggtacga tactgggata
     5341 aacaatggca tgtaatttta tatggaaatg gcaacgaaga actttttggc gatagttacc
     5401 cgggaagggt tattgccgct ggatttgatg gagtatatat ggatgtactt gatgctgcac
     5461 attattttca ggagaaaaaa taaatttttt gattttttca aactgattaa ttatatttga
     5521 ggaaggcttt ttataattta aatatgtatc acaacttgat tccacaataa tagcaaggag
     5581 ggcgttatgg gttcgctaaa gggtaagttt aaaagcttaa aggtaatact ttccacgatt
     5641 gttggattga ttgtaacaac ggcgattatt attttaatta ttatatcata taatgctgca
     5701 tattctgagc tggagaagtc gtatgtcaat cagatccaaa atgttaatag tggtattaat
     5761 atgctggtag agcaatactt tgctcaaaat attcaggttg cagagctata tgcatcaagg
     5821 gatgatgtcc gtgaatcgct tgtaatgggt aactttgggc cggtaacaaa ggaactgact
     5881 gcattcctta acaaatataa agagttgtat gaaaatatat ttatagcaac tgccgatagc
     5941 aatccagtga tacttgctgc aggtggtccc ggcgcagttg gtacacgcta tagaaatgcc
     6001 agctatgcaa gggcaattga tgaagcattg cagggtaaag taggattaag caagcccgac
     6061 aaatcaccgg tgacgggaca gccggttatc cttatacagg tgccaattag ggaaaatggc
     6121 aggatagttg gattattggg actgccactt gaattgggca aatattcata taaattagta
     6181 aaggatgtaa agattggata taccggctac ccctatgtca ctgatattga tggattggta
     6241 tttgctcatc cggaaaagga aaacatatta aagctggaga tgtctaagct ggattttgga
     6301 caaaagttgt taagcagccc tgataatacc gtcattaagt ataaatggga agggaaagat
     6361 aaaatgcttg tcggtgaaaa aaataaggga tatggtttta ttgtggcaag taccatgtac
     6421 atgtccgata tcgcaaattc tgcccgtgat atggctacaa ccatggtgct ttttggactg
     6481 atcatgttag ctggtgcagt aataggtatt tactatatca ttgcctcgcg tctcaatcct
     6541 ctggaaaaat taaaaactat agccggttca cttgccgaag gaaatgttac catgcgctat
     6601 gaaggcaggg tatacagcga tgaaattggt gagatgtcta ccgctattaa caatactatg
     6661 gaaaatttag aaaagcttgt agccgaggta aaggtagcgg taagcaattt aacgcaggcg
     6721 gttaatgaga tagccagcgg caatgaaaac ttatcgcagc gtaccagcga acaggcttca
     6781 tcactggagg aaattgccgc aacaatagaa caggctactg caactatcaa gcaaaatgcg
     6841 gatcatgcat caagagcaaa tcagatagcg gaaaagacaa gtgagctggc aaaaaatggc
     6901 aatacgctgg ttggcgatgc agtagtggca attaatgaaa taaatgcatc cagcaagcgg
     6961 ataggcgaga tacttacgct tatcaatgag atttcattcc agacaaacct gcttgcgtta
     7021 aatgcagccg ttgaagctgc acgagcggga gagcagggca gaggttttgc ggtggttgct
     7081 ggcgaagtgc gtaatttagc acaacgctca ggcagtgccg ctaaggaaat aggtgagctt
     7141 attcgcgatt cattagataa gattgatacc ggtacaacac tggtaaacaa aagtggtgag
     7201 gcgttgaagg agattatgca gtctatacag gatttgtata aaacaataac tgagattgca
     7261 acagcaagtg atgagcagaa gcagggaata gatcagataa atgtagcaat atcggatctg
     7321 gatacgatga ctcagcagaa tgcagccctg gtagaagaga cagcaagtgc aagcgaagag
     7381 atggcaaatc aggcacaaga attggcagat atgatggaac gttttgtgat tagagatgag
     7441 gtaagtcaga agatttatgg gacaaaacat aaagaattac atcttcatgc tgctgattca
     7501 gcaaaaaaac ctgtgacaaa aaaggtagat aagcaggtaa aatcaaaaga ggagaagaaa
     7561 gaagaagaaa aaaaggaaga aggcaaaaac aataagctta ttgatgatgg atttgaagaa
     7621 ttttaaattg aactatagtg cgaggtgaat catggcagag gatgccatac ggcaggtatt
     7681 tctggcagag acaaaagagt tgctggaaaa ccttgaaaat gatatagtac gctatgaaga
     7741 aactaaagat cctgaattga tacactctat tttcaggtat gtgcatacgt tgaaaggtgg
     7801 atcagggatg gccggatatg atgatgtgta tcaatttacc catgctcttg aaaatgttct
     7861 tgattcagta cgcaatggta tcatacccat ggacgatttg cttgcagata ttgtgtttgc
     7921 cagtatagat gtcatccgct tgcgtttata taccgatagt gaccagcagc agatgcttga
     7981 gaaatttaat ttgcttcaaa gtaagattaa tgatatagaa acacttttac aaaaaaagga
     8041 ggaagcagaa cctgctgaag aaccagaaga aaaaaagttt atctataatt ttttccgggt
     8101 aaaagctaaa ttcagagatg atatttttaa atttgggatt gatccactca taataatgga
     8161 agatttatct tcattgggaa agattgttga attgcgtgtt aatcgaaata atgttccact
     8221 gttgcaagac attgatccag aacaatgtta tatcgcatgg gatgtaattt taaaaaccaa
     8281 gagcaaggct gaggctctgg atgacgtttt tatatttgta cgggatgaca acgaaatcac
     8341 tattacagat gtaacaaaaa attatgtggc tgaacaggtt attgaagatg ttccaaaatt
     8401 aggtgagctt ttattatccc ggggcatgtt aacagagaat gaatttgaag atgttttgaa
     8461 cgaacacgag aagactaaaa agaaggtggg ggagatagct gttactaaag gatatattga
     8521 agaaaatgat gtatcgttag ctttaaagga acaggatgaa ataaaaaaac agataagcac
     8581 ctctactttg cgagtagatg caaaaaaagt agataatctc atgaatcttt taggtgagct
     8641 ggtaattgga ttttcaggta ttaaacgaat tgctgaagag attgaggatg agaaggctta
     8701 ccggttgaat aatgcattgt atagcgttga ccgtattata cgggaatttc aggagcatct
     8761 gatgcgtgtg cgaatggtgc caattggacc gatatttgaa caatacaggc ggtttatacg
     8821 tgatactgca aaggcgcatg gcaaatcaat acgtctggaa ataacaggtg gcgatactga
     8881 gattgacaag accgtgatag agcggattag tgatcctcta aaacatttga tacgcaatgc
     8941 tatagatcat ggaattgaag cacctcatga tagggaagaa aaagggaaac cgcgtgaggg
     9001 gcttgtctca ttaaaagcat accatcagga agggaatatg tatattgaag tgagcgatga
     9061 tggagcaggt attgataaag aaaaggtaat agaaaaagca gtggctaaag gactgctgtc
     9121 aaaaaacgat gaaattccag atagtacatt atatggtttt ctttttgaac cgggtttttc
     9181 aacctcagaa tctgttggtg atctttccgg cagaggcgtt gggcttgatg ttgtgaaaac
     9241 taatattgag gcattacgtg gtactgttga tattctcagt caacctgaaa aaggtacaac
     9301 atttattatt aaattgccat taacattagc tattattgat ggtatgctgg taagagttgc
     9361 ccatgaaaca tttattattc caatactttc aataattgaa agtattaaac ctgttaaaga
     9421 tgatattaaa acaatagaaa gtcagggtga agttgttcac atccgtggag aatatgtacc
     9481 tcttttacat ttacatcagg aatttaatct tccacaaacc atacaaaatc cctgggatgg
     9541 attggttgtt gttgttgagt ctcagggcag atcaattggg cttatagttg atgagctgat
     9601 tggtcagcag cagattgtca ttaaaagcct tgataatcag atcacaactt ccagggctat
     9661 ttctggtgct gcgatattgg gtgatggaac tatcgcactg ataatagatg ttcatggatt
     9721 tattaacagg taatatgcca ttgagtacca taaggaatct atgatgaact atcctgtaaa
     9781 agaacaatct tttaaaagcc agcatgaata tcttaaaaat cttaaaagta atgttggttc
     9841 attattttct gagataattc agcagtttac aaccatcaat aaacgaatag atgatttttt
     9901 tacaatgata cggccatata tcatctattt ttatacaacg gatgaagcta ttgctgatac
     9961 atttttgcat gatgttattt ttagtactaa ggatgagatt aatggttaca tcgaaactat
    10021 tgttaaatat atgatagctg atagggaaac caatagggaa gttgaagagg ctatcaataa
    10081 aacactcact ttaagcaaca gtgttgaaat gattttagat gctgttgagg ctatagattt
    10141 atatgctaag aataccatga tcatagcaat taaagccggg gaagaaggca caacattaac
    10201 tacaatagct gttgagatgt cacatttagc tgatatggta aatgctgttt ccagtgaatt
    10261 tcaggaaatt attgaacaac ttcattcatt gcgcacaaaa ttcagtgaaa catgcatgac
    10321 tgttgatgtt attgtagaga actatctgac tcagttacag gttagattaa ataatactat
    10381 aaatgatatt attcgacatc aaaaaaatct gtcactcaat gtaaattcta ttaagaactt
    10441 tgctgatcag ataaaagcag tgattcagaa aatcttaatg gaatttcaaa ttgaagatat
    10501 tgttcgtcag gatattgaaa aaatactctt tgctattgaa gcgcttgaag aatcaattaa
    10561 tacagatgaa aaagaacagg atgttttatt atgggggatt tacaaaaagt tattatcact
    10621 tcataatgat tttgttcatt tgcttgatag tactgtgaga gggatcaatt ttacaagaga
    10681 aagcattgta caaataaata atgattataa taaaaatgca ataaatgttt atgagggaga
    10741 tgaatttgca attgaaaaag tttataactc actggaatat ttacaaaatg agacaatcaa
    10801 atatatagat gacatattaa tgaggaaaaa gtatcttctt gatatctcaa aggatgtgct
    10861 ggtacgactg gaaaagtttc aggaattttt cagaagaata caggatgtag taagaaaatt
    10921 tgatattgtc aatatgttaa cgcgtattga attagcacgt catagagagt tacaaaaaac
    10981 cattgccagt tcactttctg atatcagcat tatgccaaaa aagattaaaa tgattgttga
    11041 agagtctgaa aatttatata aagatgtaat aagtactatg gaatcaactt atcagttata
    11101 tcagcaggcg atgtcaatac aggatgaaat tttaaaaaac tgcgtgaata atttaaaaaa
    11161 agtatctctg gaactgtacg aatcaaagaa atattatgtt gatatatccc aacagataga
    11221 aaggaatata cattcattgc aacaggtcct tgatgaaaag tcagagcaaa ttatggcatt
    11281 tcatgaaatt ggaaaaaggt tgaaattttt gtgtgatagc atggaagata tggacatttt
    11341 aaaagaaaca cctgagttgt ccactattga taaaattata gagagtatca atgcaaatta
    11401 tggtaacgat tataaaggtt taatgctgat ttccctgtta aaggagtatt caactttaca
    11461 atcagaagat actgtgattg tctttaaata acgtataaag tatgtgtttg taggaggtaa
    11521 taacgatgta tatcattgaa atagatgaat ttgtcactat aaagcacata aaacaatttc
    11581 tggatgaagt aaagactgtc ttgatgacag aaaaagatat tgtccttgat ttttctaaag
    11641 caaagcgttt agatttatca atagtacagg taatattatc gcttttgaaa acagcaaaag
    11701 ctgaagggaa aacggttaaa tttagaggag tcaatgatac tatcaaaaag cagttaaaat
    11761 tgtgtggtgt cattcgttaa ttaatttgat tacattatac ataatgaata tattacatga
    11821 gggtatagta tgaagtatat aattattatt gatgattcac ctaccatcag aacaagcgtt
    11881 gaatttactc taaaagatat tgggtttggg ctgatgcatg ctgaaaatgg tattgatgcg
    11941 ttgaataaaa tacaacagct atcggcaaat ggcgatgaag tggcaatgtg tattgttgat
    12001 ataaacatgc ctcagatgga tggaattagt tttattaaaa agttccggga aaatgacaaa
    12061 tttgctcctg ttattgtgct taccactgaa gcagaagaag agaagataca ggaagggaag
    12121 aaggctggtg cctcggggtg gatgataaag ccatttaaag cagagcaatt acaatctgta
    12181 gtccataaat ttttacgtta aggaggcaac tttatgaata ttgaaaatac tacggttgaa
    12241 aataaattag atactacatc aacagaacag tttgttacgt tcattatagg aaatgaaacg
    12301 tatggtattg aagtgttaaa ggtacaggaa attattggta tgacgcgtat tacttatgta
    12361 cccaatacgt taccctatat gaaaggagtt attaatttac ggggttcagt ggttcctgtc
    12421 attgatatgc gtaaaaaaat tggaatggat gaaattgcat ataatgcctt tacagtaatt
    12481 attatcactg aagttaaagg gaaactgata ggcatgattg ttgattcggt gcaggatgtt
    12541 gttactatac ctatccagaa gattcaggat accattcact ttacttcaca gatagcaact
    12601 gattatataa agggtattgg gcagattgat aataatttag ttattattct ggatgttgac
    12661 aggctattaa ctgcagatga gatagcatat attaaaaata tttgatatca atgtatatcg
    12721 aagtatatcg aaatgatata ttgtaaacat tatgatccga actactgatg caactataaa
    12781 caaagaagta aatattttgt tgccgggtga atattatgca tcatccgatt caatactttc
    12841 cactattgtt gggagttgtt ttgttgtttg tttatatgat cccttaaaaa aaattggtgc
    12901 aatgggtcat tgcttattgc ctgttttatc taattcgcag atgaaaaaaa atgagatatt
    12961 tcaatataat attaatttta tggaacatat tataggagaa atggtaaaat taggcgctga
    13021 tagaaaaacc tttgtggtta aagtttttgg cggcattggt aaaacgttta atcttgagta
    13081 tacaaccgat tttattaaag actattgtaa aaatgaaaat atgctgcttg aagcaattga
    13141 tgtaggtggt aactatagaa ggaaattata ttatttttgc aaaacaggga aggtgcataa
    13201 atatctggta aaacagaatg atgcattatc agaatttatt actatggaaa aagaatttat
    13261 tgataaggtt ctttccgata aagtgcaata tggtaatgtt attatatttg aataacatat
    13321 gaacaatata ataagctatc aacaattaaa tcagcgtgac tttgattact tgcgaaacat
    13381 catctatgat gaagcaggta taaagctttc ggacatgaag aaagcgctgg tacaatcccg
    13441 ccttttgaga aggatgcggg agatgggaat tagcagttat gatgaatact gtcattacct
    13501 gcgtgaaaac tatgatgaag aaatagaaaa tcttattaat tgtattacaa caaacaaaac
    13561 agagtttttc ag
//
LOCUS       DAOSOX010000051        13432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4614_length_13432_cov_8.826568, whole genome shotgun sequence.
ACCESSION   DAOSOX010000051 DAOSOX010000000
VERSION     DAOSOX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_13432_cov_8.826568"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1636
                     /gene="glpK"
                     /locus_tag="PLX22_05440"
     CDS             152..1636
                     /gene="glpK"
                     /locus_tag="PLX22_05440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002741576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOT19374.1"
                     /translation="MGKKFLIAIDLGTTGNRVFCFDEHGLPISSSYAEFTQYFPKPGW
                     VEHNAMEIWESVAQLIPDAIAKGNLNPNDAIAIGITNQRETSVLWNEETGKPIYNAIV
                     WQCRRTTDICNQLKEAGYEELFREKTGLVIDAYFSGTKVKWMLDNVKGARDLAKSGKL
                     LFGTIDTWILWKLTGGKSHKTDYTNASRTLMFNIKNKSWDLELLEILNVPRTILPEVR
                     ESASYFGETQGVPGLPDAITIGGIAGDQQAAMVGQNCVFEGTSKNTYGTGCFMLLNMG
                     NEWILSKHGLVTTLTCDNKGQPVFAFEGSVFIGGAVVQWLRDYMKFIEKSSDAEKVSL
                     SVNKEDEVVFVPAFVGLGAPYWKMDARGAIFGLTRDTTPEQIVRAAVKSTALQSYELL
                     EAMQSDSGKNIHELRVDGGATRDTYLMQYQSDILGIPLLLPEITESTALGAAYLAGIT
                     GNVYNTIDEVAKFNKIAKRYNPSMNETARQREINLWKNAVRRLL"
     gene            1700..1897
                     /locus_tag="PLX22_05445"
     CDS             1700..1897
                     /locus_tag="PLX22_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19375.1"
                     /translation="MAKPLLGEILVEDSVITKEQLNEALAIQKRDGGLIGIILMNLGY
                     INEPTLVKYLALQAERVIKSE"
     assembly_gap    2066..2165
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2166..3011)
                     /locus_tag="PLX22_05450"
     CDS             complement(<2166..3011)
                     /locus_tag="PLX22_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT19376.1"
                     /translation="MLSKQQYALLLDTFKSAQNAKNSDAFLSDIVDLLYNELKPISVS
                     VYTLVGDERLVLHHEAGKNFDELVVNIYNFSLIVRKAIKKKSVLFLPTSKPFQNDDIN
                     ISVAIIPLLHSDIVVGMICLVFPLEELQHIQNSTPFFTLIGKIAGTIRYYQTGLKVAK
                     ADTAEEEFEQKITSLRVLAQGVAHEFNNILAVIKGYAELLLMNEAVDESVKEAVAIID
                     KQTIRGSSLIDRLSVFVTGKEISFEYCSINDLIKEIVDVQRFTLEKESIECKLKLGKV
                     PEVFVD"
     gene            complement(3112..3420)
                     /locus_tag="PLX22_05455"
     CDS             complement(3112..3420)
                     /locus_tag="PLX22_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT19377.1"
                     /translation="MNFGLSNTIIEQICSVFSKHPSVEKVIIYGSRAKGNYKNGSDID
                     IALIGGEDLTVDVMFIIIEEIDDLFLPYTFDISLLRYINDHDVLDHIKRVGKVFYKKK
                     "
     gene            complement(3540..3842)
                     /locus_tag="PLX22_05460"
                     /pseudo
     CDS             complement(3540..3842)
                     /locus_tag="PLX22_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
     gene            complement(3876..4913)
                     /locus_tag="PLX22_05465"
     CDS             complement(3876..4913)
                     /locus_tag="PLX22_05465"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT19378.1"
                     /translation="MQTERTKSDLDWKNLPFGYTKTDYNIRYYYRDGKWSAGELSSEE
                     TIPIHIAAPGLHYGQQAFEGLKVFETVDKRIVAFRPEENARRMKVSCERICIPELPME
                     LFMEAVEKVVAANTKYIPPYGTGASLYVRPVIYGAGAKVGLGPASEYVFIVFATPVGP
                     YYKGGFKPVKALVVEQFDRAAPNGVGDCKVGGNYAAGLRGDEYAKKLGFPVPLYLDPK
                     EKKYVDEFGTSNFIAIKGNTYLTPESSSILPSITNKSLMDIAALLGMNVEKRPIAIDE
                     VEHFDEVGAVGTAAVVSPVCSIHYRDKIFTFCDEDKAGPVLTRLYEYLTKLQTGEIKD
                     TFGWLHEIVVK"
     gene            complement(5158..5910)
                     /locus_tag="PLX22_05470"
     CDS             complement(5158..5910)
                     /locus_tag="PLX22_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT19379.1"
                     /translation="MIALEHVQLLFNNHYVLQDVNLFVQQGSTYVILGKNGSGKTVLL
                     KTIAGLFPQFSGTIAINGISLHEYTQLPINEKIHKNITMGYVFQKGGLFDSMTVYDNV
                     AFGLQRRASTEDEIKNKVLSALEWVGLAGSENKLPSQLSGGMQKRVGLARALCLSPAI
                     ILYDDPTAGLDPVLTDSISELIVATQNKYAMTSILVTQDLAVAEKTAHRIGLLHNGMI
                     IVDCSADEFFSKTNPYAKQFIDGDSEGPIEII"
     gene            complement(5907..6839)
                     /gene="rsgA"
                     /locus_tag="PLX22_05475"
     CDS             complement(5907..6839)
                     /gene="rsgA"
                     /locus_tag="PLX22_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT19380.1"
                     /translation="MKGVVSKVMGLYCTVYCDAIYINCVMRGKLRQLIEAGSKKFSNI
                     IAVGDEVVITKLDDGSGVIEEILPRRNYFSRKEKGKHKKIDMIAANCDCVVAMQSFAL
                     PRLNLRFVDRIAVRCVEQNIPLVVCINKCDLAKKKDYEYVTNYYKSTDVTVIISSVNT
                     SYNIHALQDILHQKTSVIVGYSGVGKTSILNAMYPQLSLVVGEVSGSTGKGKHTTTNV
                     SMIIIDETTRIIDTPGLREFGIMDIEPHVLSKYFYEFDNYAPKCSFKPCTHDHEPDCE
                     IKRLVQKGIICEERYISYRNILYSLKDYYATMYS"
     gene            complement(6941..7441)
                     /locus_tag="PLX22_05480"
     CDS             complement(6941..7441)
                     /locus_tag="PLX22_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT19381.1"
                     /translation="MKNVLAVMSVFAFAFMVACSSTPAVQQPAGDPNVALVNAQNKQL
                     ERLPVTGFAYKSSVVPKQEWDKWAEAAAPVIKEVINKLPEGYVLQVTGHTDASGPEEA
                     VGNKPGNLKISTDRAKAVYNALKAKGIDSPRMTYKGVGSSELLPGVDPRDGANRRVTF
                     KVVPKK"
     gene            complement(7764..8126)
                     /locus_tag="PLX22_05485"
     CDS             complement(7764..8126)
                     /locus_tag="PLX22_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19382.1"
                     /translation="MKKIVIGVFLLLLTNSYAQSFLYELSDKIDDYKNKNITMIMKLK
                     YYDTLFQVITFYDAKNHDISFDCIDVMKHPWFLQQKNNLHPGMEYNVTFTVKGLGNMK
                     EIIAQLQYFEPVILQKIP"
     gene            complement(8878..10899)
                     /locus_tag="PLX22_05490"
     CDS             complement(8878..10899)
                     /locus_tag="PLX22_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT19383.1"
                     /translation="MVLLTRFFWDNNDMYSINVKAIKILMLNCIIVLISCSAHSPGVF
                     QSLEGTWKLATKNIEYDYSNKRLPEYVEINIPCSIKVPHTFENNNMQLPVKGYVWIQK
                     EIFIESLPANDLALQFGEIMNADVAYFNGKQIGASGRFPPDFKSAWGKFRHYTIPRDI
                     INKGANVIALKVYYDSEFWITPPVRLIDNVAGSKIASLLDYLRIQLMENIFILLILLS
                     FFFLMMFYFQREYMEYLLFGIACFTIAMPQALHFLENLYPDIGLTSDTILKLTQPGLI
                     LFPAFLNLFYRYHIGKQKKWLTIVYIAVPIVFSTIMVLQQTRAGILLYRNLSIIVSIV
                     YMIDLFILSGWQVLHKKKAGALIFIGLVPMVVLGIHDVLAFGLHIIPSYIVLYVYGLP
                     FLIFIIAIYLVNIFVKALKEAKDLNIQLQKLLLDAKRLAYLEKELEIAKAIQSQLLPS
                     KIPNIEGIDIAIKFQPAMKIGGDIYTIFQIKDGVVFFVADVVGHGIPAALISAMVNVL
                     LKMFQMLAIDPALLLRSLNVNICTMLPQTFVTAVCVYCNRSHNFMRIARAGHPPMIKC
                     SNDGNISMYMPSGRALGIQTQTRFKTVELTMEKGDRLIIYTDGLVEASLPGNSKDIYG
                     EQRLKNMIQQTINLPLDKAIEVVYNDVISYVGSPQYLTDDITVIMIEIL"
     gene            11084..12052
                     /locus_tag="PLX22_05495"
     CDS             11084..12052
                     /locus_tag="PLX22_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HOT19384.1"
                     /translation="MKTKEFISLKWSLYEKNPIIKYPFPSPIIADPSFITPDESKDGK
                     WHLFAHSLLGIHHYISMDGITYTKLKKQTLRRAMRPFLLYDNGMYYLYYEKTKPFILW
                     YSWLPHQWYSYIAMRSSKDCYTWSNEKIILKPSLPWHHNNLGYSISNPCVVKTKSTYY
                     LYYSAALAYIMDCGFNEPLHIGLAKAQSPEGPFECCSEPILSPSQYNRWCNLGCGSFK
                     VLAFDDGFIGLQNGIYWDYTINHSGSAILVYSSKDGKTFKTLLKDPLLKPDNINGSFM
                     KSHVYAFDAKPVDKEQWFLYFNARNDWHWSKGREHIGLLIGKKNKH"
     gene            complement(12049..12990)
                     /locus_tag="PLX22_05500"
     CDS             complement(12049..12990)
                     /locus_tag="PLX22_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOT19385.1"
                     /translation="MLKDISENGNYQKHLENIVKTLCQPQSYKKVYHRINHDVPMPSI
                     QQLEEIVEECKMVLFPGYFGNSEITPHNMQYYIGSAIDGIARKLTEQIKRGYCFSCSE
                     NETIDCIECEHRSRKIMFQFLDMLPEIRRMLAMDVQAAYEGDPAAKSHGETIFCYPSI
                     TAMIHYRIAHALYILDVPLIPRIITEMAHSQTGIDIHPGATISENFFIDHGTGVVIGE
                     TTIIGNNVRIYQGVTLGAKSFPLDENGNPMKGIPRHPIVEDNVIIYSGATILGRVTIG
                     KGSVIGGNVWLTESVPPKTHILQQKPSEMMFEHGGGI"
     gene            complement(13086..>13432)
                     /locus_tag="PLX22_05505"
     CDS             complement(13086..>13432)
                     /locus_tag="PLX22_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT19386.1"
                     /translation="EKIIIFMDEAFHLVEREKEYFHVLIDFWTQINHNERMRKANIKL
                     YQSYRAECAKIIQEGIDKNVFKDVDVQYTATMIVAFVQGLIIQYIIDNEAFDYTEYTK
                     KIKLQIVDNLMK"
BASE COUNT         4244 a         2680 c         2135 g         4273 t
ORIGIN      
        1 tatatactct atgttgtatc aatctcatga tgattaacca tcgttgaaac aagctatcag
       61 ctataaaatt tattacttgc attatagctt tttttaaaca atgattgcat accatgaact
      121 atcgtctaag aaaataccat cggagatcgc tatgggcaag aaatttctta ttgcaattga
      181 tttaggcact acagggaacc gtgtattctg ctttgatgaa catgggcttc ccatctccag
      241 cagttatgct gaatttacgc aatattttcc aaaaccaggc tgggttgaac acaatgccat
      301 ggagatatgg gaatcggttg cacaacttat ccctgatgct attgcaaaag gaaatttaaa
      361 tccaaacgat gctatcgcta ttggaataac aaatcaacgg gaaacttccg ttttgtggaa
      421 tgaagaaacc ggcaaaccaa tctacaatgc tatcgtatgg cagtgccgca gaactaccga
      481 tatatgcaat cagcttaaag aagctggcta cgaagaacta ttcagagaaa aaacagggct
      541 tgtcattgac gcatattttt cgggcaccaa agtaaagtgg atgcttgata atgttaaggg
      601 ggctcgtgat ttagcaaaaa gtggcaaatt gttatttgga actatcgaca catggatttt
      661 atggaaactt accggtggaa aatcacacaa aaccgattat accaatgcat cacgaacatt
      721 gatgtttaat atcaaaaaca aaagctggga cctggagctc cttgaaatac tcaacgtacc
      781 gcgcacaatt ttgcctgaag tgagggaatc tgcctcatat tttggcgaaa cacagggagt
      841 gcccggattg cctgatgcaa ttaccatagg tggcatagcc ggtgaccagc aggcagcaat
      901 ggtaggacaa aactgcgtat ttgaaggaac ttcaaaaaat acctatggta ctggctgttt
      961 tatgcttctt aacatgggaa atgaatggat actgtcaaaa cacggcctgg ttaccacatt
     1021 aacctgtgac aacaaggggc agccagtctt tgcatttgag ggctcagtat ttattggcgg
     1081 tgctgtagtc caatggcttc gtgattatat gaaattcatt gaaaaaagct ccgatgctga
     1141 gaaggtttca ctttcagtaa ataaagaaga tgaggttgta tttgtcccgg catttgttgg
     1201 attgggtgca ccttattgga aaatggatgc acgcggtgca atctttggct taacccgtga
     1261 tacaacaccc gagcagattg ttcgtgctgc ggttaagtcc actgctttgc aatcgtatga
     1321 actccttgaa gctatgcaaa gcgattcagg aaaaaacatc catgaactca gagtggatgg
     1381 aggtgcaacc cgtgacacat acctgatgca atatcagtct gacatactgg gtataccgct
     1441 cttgctaccc gaaattaccg aatcaacagc attgggagca gcatatttag caggaattac
     1501 cggcaatgtt tataatacca tcgacgaagt agcaaaattt aataaaattg caaaacgata
     1561 taatccatca atgaatgaaa cagccagaca gcgtgaaatt aatttatgga agaatgctgt
     1621 caggcgatta ttataattta cttatcggta taacattgaa tataatgtac atcaacattt
     1681 actcttgcaa ggaggcacta tggctaaacc actattgggt gaaatacttg tagaagattc
     1741 tgttataacc aaggagcagc tcaatgaagc tcttgctatc cagaaacgtg atggagggct
     1801 tattggtata atacttatga atttaggata tatcaacgaa ccaacattgg tcaaatattt
     1861 agcattgcag gcagaacgag ttatcaagtc agagtaaggg caggctttat tcctgtaaag
     1921 tgttgtatct gcgtgccata atctcattgg taagtttaat aattgtatct atcttgaacg
     1981 gttttcggag aacaccctct aagcccatca tcaatagttt gtcgatatca ggtattgaat
     2041 caactcctgt aataaataca acatcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnatcta caaatacttc cggaacttta cctaatttta atttacattc aattgattct
     2221 ttttccagtg taaatcgttg tacatccact atctctttga ttaaatcatt tattgaacaa
     2281 tactcaaaag aaatttcttt gccggtaaca aatacactaa gcctgtcaat gagtgaagag
     2341 ccacggatgg tttgcttatc aatgatagca actgcttcct ttacggattc gtcaacagct
     2401 tcattcatca gcaaaagttc agcatatcct ttaataaccg ccagtatatt attaaattca
     2461 tgtgccacac cctgcgccaa tacccttaag cttgttatct tttgctcaaa ctcctcttca
     2521 gcagtatcag ctttagctac tttaagccct gtttgataat atcgtattgt gcctgctatt
     2581 ttacctatga gtgtgaaaaa tggtgtgcta ttttgtatat gctgcaattc ctccaatggg
     2641 aacaccagac atatcatccc caccactata tcagaatgca aaagaggaat aattgcaacg
     2701 gatatgttta tgtcatcgtt ctggaatggt tttgatgttg gtaaaaataa tacgctcttt
     2761 tttttaattg ctttacggac aataagcgaa aaattataaa tattaacaac cagttcatca
     2821 aaattcttac cagcttcatg atgcaatacc aaacgttcat cgccaaccag cgtatataca
     2881 gaaactgaaa taggttttaa ttcattataa agtaaatcaa caatatctga aagaaaagca
     2941 tcactgtttt ttgcattttg ggctgattta aaagtgtcaa gcaataaggc atattgctgt
     3001 ttacttagca tatataattc cgggtacaat tatttctata agaatctatt agagactata
     3061 gcacacaatc tatttttatt caacaaatgt aggaaaattg cggtaagggt tttatttctt
     3121 tttgtaaaat actttaccaa ctcttttgat atgatccagc acatcatgat cattaatata
     3181 tcgtaataat gatatatcaa atgtataggg taaaaaaaga tcatcaattt cttctataat
     3241 aataaacatg acatcaaccg taagatcttc tccaccaata agggcaatat caatatctga
     3301 accattttta taattacctt ttgccctgga accatatata ataacttttt ccacactggg
     3361 atgtttagaa aaaacagaac aaatttgttc tattatagta ttgcttaatc caaaattcat
     3421 gatatctggt tgttatatag taatttaatt ttttctataa aacgtttcaa ctcctcgaaa
     3481 taactttctt ttatcctctt tattatctcc tgagcaagtt cactatgata ggtacgttat
     3541 gtcaaatttc tactttttat catatccatc cagatttcac cattctcaat taatcctcgc
     3601 ctgaaggctt ctctggttgt atcttttgaa ccatataaag tctgaatacc ctgctcttca
     3661 aggaaatctt taaacacatt ccatgccagc tcatgcgtat attcaaaact ttgtatcaag
     3721 ccttgctcct ccaattctgt taatgaacgc tgattagcaa gatgaattgc tttttctaat
     3781 tgaagaaacg cttgcatgag atgtttaaac ctttgtatcc agcagatatc ttgattattc
     3841 atgctatcta tgctccagaa tgattaaaaa agactttact taactacaat ctcatgcaac
     3901 cagccaaagg tatcttttat ttcacctgtc tgcaatttag tcagatattc ataaagcctt
     3961 gttaataccg ggccagcttt atcctcatcg caaaatgtaa atattttatc acgataatga
     4021 atactgcata ccggtgatac taccgctgct gttccaacag cgccaacttc atcaaaatgc
     4081 tctacctcat caatcgcaat tggtcttttt tcaacattca tccctaacag cgcagcaata
     4141 tccattaaac ttttattggt tatactgggt agtatcgaac ttgattcagg agtaagatag
     4201 gtattacctt tgatcgcaat gaagtttgat gttccaaatt catctacata tttcttttct
     4261 ttggggtcaa gatacagcgg aacaggaaat cctaattttt tagcatattc atcgccacgc
     4321 aaaccagcag catagtttcc tccaacctta caatcaccaa caccattggg agcagctctg
     4381 tcaaattgtt ctactaccaa tgctttcact ggcttaaaac cgcctttata atacggtcca
     4441 acaggtgtag caaatactat aaaaacatac tcacttgccg ggccaagccc tacctttgca
     4501 ccagcaccat atataaccgg gcgcacatac aaactggccc ctgtcccata cggcggaata
     4561 tattttgtat tagcagcaac aactttctcc acggcttcca taaacaattc catagggagt
     4621 tcaggaatac atatacgttc gcagctaacc ttcatacgtc tggcattttc ctcaggtctg
     4681 aatgctacaa tacgcttatc aactgtttca aataccttta acccctcaaa agcctgttgc
     4741 ccatagtgaa gtcctggagc agcaatatga attggtatag tttcctctga tgacaactcg
     4801 cctgcagacc atttaccatc ccggtaataa tatctgatat tatagtctgt tttagtatat
     4861 ccaaatggga gattcttcca gtcaagatca gattttgtac gttcagtttg catcttttta
     4921 acccccataa aaaataaaaa ccatacataa tgatatggct aaaaatataa tacgccatta
     4981 ttattcaatg tagcaattaa tgagcatgct gtcaaatata ttttgttcat aaatctaact
     5041 atcaatcact tccattcatt gtttcatagt tcatcaaata acgattgaag agcgtaccac
     5101 atcaacaatt acatcactgc gacgatgtac attcacatta tagtagcacc atgctgttta
     5161 tataatttct attggtcctt cgctatcgcc atctataaat tgttttgcat aggggtttgt
     5221 tttactaaaa aattcatctg cactgcaatc aacaataatc atgccgttgt gcaataaacc
     5281 tatcctgtga gctgtttttt ccgcaactgc aagatcctga gtaactagaa tagatgtcat
     5341 tgcatattta ttctgtgtgg caacaataag ttcggatatg gaatctgtta gtaccggatc
     5401 aagaccagca gttggatcat catacaatat aattgcaggc gacaaacaca atgctcgtgc
     5461 aagccctacc cttttttgca taccaccgct aagctgtgaa ggaagcttat tttctgaacc
     5521 ggccaatcca acccattcca atgctgacag cactttattt tttatttcat cttcagtgct
     5581 cgctcgtctt tgcaaaccaa atgcaacatt atcataaacc gtcatcgagt caaacaaccc
     5641 gcctttctgg aatacatatc ccatggtgat attcttatga atcttttcat tgataggcaa
     5701 ctgtgtatat tcatgaagcg atattccatt tatggcgata gttcctgaaa attgggggaa
     5761 cagcccggca attgttttta acagcacagt tttaccactg ccatttttac caaggataac
     5821 atacgtgctc ccctgttgaa caaacagatt tacatcctga aggacataat gattattgaa
     5881 caatagctgt acatgctcta atgctatcat gagtacatcg ttgcataata atcttttaat
     5941 gaatagagaa tattacgata cgaaatatat cgttcttcac atataatacc cttttgtacc
     6001 aaacgcttta tttcacaatc aggttcatgg tcatgggtgc acggtttaaa agaacattta
     6061 ggcgcatagt tatcaaattc atagaaatat ttgctcaata catgtggttc aatatccata
     6121 atgccaaatt cacgcaatcc cggagtatca ataattcgtg ttgtttcgtc aatgataatc
     6181 attgaaacat tagtcgttgt atgcttcccc ttgccggtgc ttccagaaac ctcacccact
     6241 accagtgata attgtggata catagcattc aggatgcttg ttttaccaac acctgaatat
     6301 ccaactatta cagaagtttt ttgatgcaaa atatcttgta gtgcatgtat attatagctg
     6361 gtattgacac tggaaatgat tacggtaaca tcagtacttt tatagtagtt agtaacatat
     6421 tcataatcct ttttttttgc cagatcacat ttattaatac ataccaccag cggaatattc
     6481 tgctcaacac atcgtactgc aatcctatcc acaaaccgca aatttaatct tggcaatgca
     6541 aacgactgca ttgccaccac acaatcgcag tttgcagcaa tcatatctat ttttttgtgt
     6601 ttcccttttt ccttccgtga aaagtaattc ctccgtggta atatttcttc tattacaccg
     6661 ctaccatcat caagttttgt tattacaact tcatcaccaa ctgcaatgat atttgaaaat
     6721 tttttacttc ctgcttcaat taattgtcgc aatttaccgc gcataacaca atttatatat
     6781 attgcatcgc agtaaactgt acaataaagc cccattactt tggaaacaac acctttcatg
     6841 aactatcata ccagaattaa ataatataaa caaatagagc cagttttatc ctggctctat
     6901 atttttttag taatttataa acacataaat ggttaaaacg ttatttcttt ggaacaacct
     6961 taaaggtaac acgtctgtta gcgccatcac gcgggtcaac gcctggtaaa agttcggatg
     7021 aaccaacacc tttgtaggtc atcctgggtg aatcaatgcc ttttgctttc agagcattat
     7081 atacagcttt tgccctgtca gttgaaatct ttaaatttcc aggtttattc cccactgctt
     7141 cttctggtcc tgaagcatca gtatgacctg ttacctgtag cacataccct tctggtaatt
     7201 tatttatcac ctcttttata accggagcag cagcttcagc ccatttatcc cactcctgtt
     7261 ttggtactac tgaactttta taggcaaaac cagttaccgg taatctttca agctgcttat
     7321 tctgtgcatt aaccaatgct acatttggat caccagcagg ttgttgtaca gcaggtgtac
     7381 tgctacacgc caccatgaat gcaaatgcaa atacactcat tactgccaat acatttttca
     7441 ttgtaacact ccttcttata atttttttta ttaataaaaa tgcaacctct ttttctttat
     7501 aaaagataca tgttttatac atactgtgtc aagtagtttt ttaatagcta aaatattttt
     7561 tatttaagat tttattaaac cttaccgata ataataactg ataagggcac aagagtaaat
     7621 ccctcccgat cactactttt tgtgctcaag gcactgcgca tacgtttacc cattcagggg
     7681 aaggcaggat agccgcccag ctatcttgcc tttcgttttt ttagtaaata ggcctatctc
     7741 attgtctttg catcaatgta ttctcaagga attttttgta aaatcactgg ttcaaaatat
     7801 tgtaactgag caatgatttc tttcatgtta ccaagacctt ttacagtaaa tgtaacatta
     7861 tattccatac cgggatgcaa attatttttt tgctgtaaaa accatgggtg tttcattaca
     7921 tcaatacaat caaatgatat atcatgattt tttgcatcat aaaaagtaat aacctgaaat
     7981 aatgtatcat agtattttaa tttcataatc atagttatat ttttattttt ataatcatca
     8041 atcttatcag atagttcata taaaaaactt tgagcataac tatttgttag taaaagtaaa
     8101 aaaacaccga tcactatctt cttcatagtc aatcctccag caacgtatta taaaaacccg
     8161 cttattctta tcgatagtat aatctcaata atgtcttaaa ccattcgtca atcacaatat
     8221 atctatagta tttatacaat gaagagtaaa ttgcccctcc aaaaaaatag aattattcct
     8281 cattgtcatt cagggtttga tcatgaatcc ccttttttaa ccttctgcca ttctggacct
     8341 gttccagaaa ccacctatat acgtgagatc ctgaatcgtg gctcagaata acaagccacc
     8401 acatgttcat cccgtgattg gcaaaccagc caaaccatga gttaccacaa acgggatacc
     8461 ggcaattcct taaaaaaact gcagccacta aagctgcagt taaaaataaa aactgggatt
     8521 gattgttatg aataaacgga ttgtatccta tcggataagc tttgattaat gccccctctt
     8581 atgactgcga taagcaatat attcaataat aacccaaaga agcacacata cccagaaaag
     8641 agcaaaaaac tgtaaaattg gtatattcat catggttatg ctcctccaac gttctattta
     8701 ttatacaagc aattatcatg ccaacttaaa atatttttta caaatggaaa tgctggaaaa
     8761 ttcagggttt ttgtttttgg cgatagtaac tgataataga ttttttttga ttattgccac
     8821 accgtgtcaa atttgcaaca tcaatgacta actattgatt tagccgacac tcagtattta
     8881 cagtatttct atcatgatga ctgtaatgtc atcggtaaga tactgagggg aaccaacata
     8941 tgatatgaca tcattatata ctacctcaat agctttatcc agaggaaggt ttatggtttg
     9001 ttgtatcata ttttttaatc tttgttcacc atatatatcc ttgctatttc ccggtaacga
     9061 tgcttcaacc agaccatcag tataaataat taatctgtcc cctttctcca ttgttaattc
     9121 aacagtttta aatcgcgttt gtgtttgtat acctaatgct ctccctgatg gcatatacat
     9181 agaaatattc ccatcattac tgcactttat cataggagga tgtccggcac gcgctatgcg
     9241 cataaaatta tgtgatcggt tacaataaac acataccgca gttacaaatg tttgagggag
     9301 catggtgcat atatttacgt ttaagcttct tagcaaaaga gcaggatcta tagcaagcat
     9361 ctgaaacatt ttaagcagca cattaaccat tgctgaaatc aatgccgccg ggatgccgtg
     9421 tccaacaaca tcggcaacaa agaacactac gccatccttg atctgaaata tagtatagat
     9481 atctcctcca attttcatag ccggttgaaa cttaatagca atatcaatac cttctatatt
     9541 tggaattttt gatggtaaca attgactttg tatagctttt gctatctcca gctccttttc
     9601 cagatacgcc aatcgttttg catcaagcaa tagcttctgt aactgtatat ttaaatcttt
     9661 tgcttctttt aaagctttta caaaaatatt tactaaataa attgcaatga taaatatcaa
     9721 aaatggcaat ccgtaaacat ataacactat atacgaaggt ataatgtgca gtccaaatgc
     9781 taaaacatca tgaattccaa gaactaccat gggcactaaa ccaataaaga tgagcgctcc
     9841 cgccttcttt ttatgcaaca cctgccatcc tgataatata aacaaatcta tcatataaac
     9901 aatggatact atgatcgata gatttctata caataatatt cctgcccttg tttgctgcag
     9961 caccataata gtgctgaata ctatcggcac agcaatataa actatagtta accatttttt
    10021 ttgtttcccg atgtgatacc ggtaaaacag attaaggaaa gccgggaata atatcaatcc
    10081 aggctgtgtt agcttcagaa ttgtatcaga cgtcaaccca atatcaggat acaaattttc
    10141 taaaaaatga agtgcctgtg gcatagcaat ggtaaaacac gctatgccaa ataataaata
    10201 ttccatgtac tcccgctgga agtaaaacat catgagaaaa aaaaatgata atagtattaa
    10261 caaaatgaat atattttcca tcaattggat tcttaaataa tccaacaagc tggcaatttt
    10321 actccctgca acattgtcaa tcaacctgac aggaggggtg atccagaatt ctgaatcata
    10381 gtaaactttt agtgcaataa cattagcgcc tttgtttata atgtctctgg gtatagtata
    10441 atgacggaat ttaccccatg cagatttaaa atccggcggg aaccgtcctg acgcccctat
    10501 ttgtttccca ttgaagtaag caacatcagc attcatgatt tcaccaaact gcaatgccaa
    10561 atcattagct ggcaaagatt caatgaagat ttctttttga atccatacat atcctttaac
    10621 aggcagctgc atattgttat tttcaaatgt atggggtact tttattgagc agggaatatt
    10681 aatttccaca tattcaggca accttttatt ggaataatca tactctatat ttttagtagc
    10741 aagcttccat gtgccttcca gcgactggaa cacaccaggt gaatgtgctg aacatgatat
    10801 caaaactata atgcagttaa gcattaaaat ttttatcgcc tttacattaa tggaatacat
    10861 atcattattg tcccaaaaaa aacgtgttaa tagtaccata tcaccagcat tatgtcaaag
    10921 gcttttcata ttcaggtctt aaatatatgc gacagttttt ttaatataca attattataa
    10981 taaatgcact tgactaaatg gaatacaaaa gttaccaata atagtaacta tcgaacatac
    11041 agcattaaaa aattgcagtg cattcacaat gaaaggaaca gttatgaaaa caaaagaatt
    11101 tataagtttg aaatggtcgt tgtatgaaaa aaatccaatc ataaaatatc ctttcccatc
    11161 gccaattatt gctgacccat catttattac tccagatgaa tcaaaggatg ggaaatggca
    11221 tctttttgct cactctttgc ttggcattca ccactatatt tctatggatg ggattaccta
    11281 tacaaaatta aaaaagcaga ctcttcgcag agctatgcgt ccgtttttgc tttatgataa
    11341 tggcatgtac tatttatact atgaaaaaac taaacccttt attttatggt actcatggct
    11401 cccccatcaa tggtactcat atatagcaat gcgctcttca aaagattgtt atacatggag
    11461 taatgaaaaa attattttaa aaccatcatt accatggcat cacaacaatc ttggatattc
    11521 aataagcaac ccctgtgttg ttaaaactaa gagtacatat tacctctact atagcgctgc
    11581 attagcatat ataatggact gtggctttaa tgaaccgcta catattggat tggcaaaagc
    11641 acaaagcccg gagggcccct ttgaatgctg cagcgaaccc attctatcac cttcgcaata
    11701 taatcgatgg tgcaatctgg gatgtgggtc atttaaagtt ttagcatttg atgatggatt
    11761 tataggtttg caaaatggca tatactggga ttatactatc aatcattcgg gttcggctat
    11821 acttgtctat tcttcaaagg atggcaaaac atttaaaact ttattgaaag acccgttact
    11881 gaaaccggac aatatcaatg gatcatttat gaaaagccat gtatatgcct ttgatgcaaa
    11941 gcctgtggat aaagaacaat ggtttttata ttttaatgcc cgcaatgatt ggcactggag
    12001 taaaggcaga gagcatatcg gacttttaat tggtaaaaaa aacaaacatt aaataccacc
    12061 accatgttca aacatcatct ctgatggctt ttgttgcaat atatgggttt taggtggaac
    12121 gctttcggta agccacacat tgcctccaat gaccgatccc ttcccaatgg taacccttcc
    12181 caatattgtt gctcctgaat aaataatgac attatcttca acaataggat ggcggggaat
    12241 tcctttcatg gggttaccat tttcatcaag cggaaaactt tttgccccca atgtaacacc
    12301 ctgataaatt ctaacattat taccaatgat agttgtttca ccaataacaa caccggtacc
    12361 gtgatctata aagaaatttt cgctgattgt tgcaccagga tgtatgtcta tcccggtttg
    12421 cgagtgagcc atctcggtga ttatacgcgg gataagcggg acatccagta tatagagtgc
    12481 atgggctatc ctgtaatgta tcatggcggt tatactggga tagcaaaata ttgtctcacc
    12541 atgacttttt gctgctggat ctccctcata cgctgcctgc acatccattg ccagcatgcg
    12601 acgtatttcg ggtaacatat ccagaaattg aaacattatc ttccgtgaac gatgctcgca
    12661 ttctatgcaa tcgatagttt cattttccga acatgaaaaa caatagccac gttttatctg
    12721 ttcagtaagt tttcgggcaa tcccgtcaat agctgaacca atataatatt gcatattgtg
    12781 aggagtaatt tctgaatttc caaagtagcc aggaaaaagc accattttac attcttcaac
    12841 aatctcttct aattgctgaa ttgaaggcat gggaacatcg tgatttatcc ggtgatatac
    12901 ctttttatat gactgaggct ggcataatgt ttttactatg ttttctaaat gcttctggta
    12961 atttccattt tctgaaatat cttttaacat ataattctgt aatcctgata taccattacg
    13021 accatgtttg gtcagtaagt ttatcgcaat atagcataca aatcaaaatt ttgcaacacc
    13081 cttgattact tcatcaagtt atccactatc tgcaacttaa tcttctttgt atattcagta
    13141 taatcaaatg cttcattatc aataatatat tgtataatca gtccctgaac aaaagcaaca
    13201 atcatcgttg cggtatattg cacatccaca tctttaaata catttttatc aataccttcc
    13261 tgtattattt tagcacattc tgccctatag ctctggtata atttaatgtt tgcttttctc
    13321 atacgctcat tatgatttat ctgtgtccag aaatcaataa ggacgtggaa atattctttt
    13381 tcacgctcta caaggtgaaa cgcttcatcc ataaagatta ttattttttc tt
//
LOCUS       DAOSOX010000052        13276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4698_length_13276_cov_8.647001, whole genome shotgun sequence.
ACCESSION   DAOSOX010000052 DAOSOX010000000
VERSION     DAOSOX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_13276_cov_8.647001"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1187)
                     /gene="hflX"
                     /locus_tag="PLX22_05510"
     CDS             complement(15..1187)
                     /gene="hflX"
                     /locus_tag="PLX22_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT19387.1"
                     /translation="MDASVNRIPISEKERAILIGVLLPEADESEISHSLDELTQLVHT
                     AGAVVVTRYIVKRMAFDPAYLIGMGKLTEIKNLIAEQSIKLVVFDLNTIRPVQIRNLE
                     NYLHCRVIGRTEVILDIFARRARTTEAKIQVELAQLNYILPRLKGLGGVLSRLGGGIG
                     TRGPGEKMLETDRRHILRRITTLKKKLIHISHRRDLQRQQRLHNEIIGAVVGYTNAGK
                     STLVNILAKDDLFVEDRLFATLDPYTRSVYLDNQHNVLLTDTVGFINNLPSHLIESFK
                     STLEEIKYSDFIIHVVGLDINIDRAVAVVAHELQLLDSNNKPNILFFNKCDIVSPDVI
                     HHVRLLYPDSIIGSCLTGEGIINLKERMIKIIETTHHKKQQSIINKNIELKYHHLN"
     gene            complement(1168..1611)
                     /locus_tag="PLX22_05515"
     CDS             complement(1168..1611)
                     /locus_tag="PLX22_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOT19388.1"
                     /translation="MIYGYAFLTNHSGYIYANDKRHCIITIVNTLNEKIQCNVEIALT
                     DEEHATGLMNRTALCDDCGMLFVFADETYRTFWMKDTAIPLSIAFIDANGIINDMHDM
                     NPFQTFPEYSSKYPARYALEVNKGWFKKNAISVGSKVIMHGCISK"
     gene            complement(1702..2433)
                     /locus_tag="PLX22_05520"
     CDS             complement(1702..2433)
                     /locus_tag="PLX22_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19389.1"
                     /translation="MQKLIMIIMCILCFDVAYAQYYVRNTTVIDTPTAYTIARGTYQL
                     SFLGYDNGGVELKAFLGLHNNFYLGASFDCQSAIGKDDPDPNVPGVVGKIKFTDGWEM
                     FPISFAIGYDSFYIGEEGKTYNSKNDLDQVIYGPYFVITKPIYLFDDEQHIHFGMRMP
                     TQPDYVPEDTSYFLSFDIPLGDMFVFKAEMERVYYNFSNPEDWLYNVGMRYSYMQRLG
                     IEFDVLFQKHENPNRIIRIEYINEF"
     gene            complement(2500..3021)
                     /gene="rph"
                     /locus_tag="PLX22_05525"
     CDS             complement(2500..3021)
                     /gene="rph"
                     /locus_tag="PLX22_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT19390.1"
                     /translation="MLPYATVPRTERNRIKPDGRSVEIQRLIGRCLRTVVDLTALQNI
                     ALVVDCDVLQADGGTRTAAITGAYIALQIAIQKLIHRNVIDVNPVIAMVAAISAGYIG
                     DSLLLDLDYAEDSQCDVDMNLVMDSNYQIIEIQGAGEKRSYAFHELDSMITIAKKGIE
                     KLFQIQNEILKLC"
     gene            complement(3036..3233)
                     /locus_tag="PLX22_05530"
     CDS             complement(3036..3233)
                     /locus_tag="PLX22_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19391.1"
                     /translation="MRKNKRQYNEMREVTVYTDYCPNALGSVLYQCGNTQVICAVSCS
                     NQVPQHALDKGTGWLTAEYNG"
     gene            complement(3205..>3750)
                     /locus_tag="PLX22_05535"
     CDS             complement(3205..>3750)
                     /locus_tag="PLX22_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19392.1"
                     /translation="GVSYKMSHFFGINVDFIENAINSSDLYDFTSGFNESFRVNTGIE
                     LNYLDILFFRTGYRFNDAGTYTFGMGFNYAIKNVAFVIDASYQDAGVFGPIYSFTVTF
                     KLIPRVITIEDKLKAEAHYQRGIKYFITDDIDGALEEFKKASDYDPYHKNVDKKIKDL
                     EELKELKRQNELLEKEQKTVQ"
     assembly_gap    3751..3850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3851..4200)
                     /locus_tag="PLX22_05540"
     CDS             complement(<3851..4200)
                     /locus_tag="PLX22_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HOT19393.1"
                     /translation="MINYKKYILYTLSLLLLILFIIINAINTIFAAGSGGTSGADFLE
                     IGVGSRPLGMGEAFTAEVNDINAIYYNPAGLATLKFPVMSIMHQELILDSRFENISLA
                     LPLYGGFFGFSTSV"
     gene            complement(4448..5563)
                     /gene="prfB"
                     /locus_tag="PLX22_05545"
     CDS             complement(4448..5563)
                     /gene="prfB"
                     /locus_tag="PLX22_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_120190971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT19394.1"
                     /translation="MIETKDLLRELSQIKNDIGELYTTLNIDDKKKQLSLLIEETFKE
                     NFWQDQAHCTQIQQQITQFKRTIEPLVALKQEVEDSIVLLEMAIDEKDNDVMKDIGSK
                     IEQFRNRFEQLETYELLSGADDDKNAIMTIHPGAGGTESQDWASMLMRMYMRWAEDND
                     FEVEVLDYMPDIEAGIKEATLLIKGQYAYGFLKGESGIHRLVRISPFDSNQRRHTSFA
                     SVDVIPEAPDNIDIDIKESDLRIDTYRASGSGGQHVNRTDSAVRITHIPTGIVAQCQN
                     ERSQHKNKAYAMKILKSRLYERKRQELEKEKMEKFGEKKDIAWGNQIRSYVFHPYTMV
                     KDHRTGEETGSVEYVMDGEINKFIYAYLKSLKNINRK"
     gene            complement(5652..6782)
                     /locus_tag="PLX22_05550"
     CDS             complement(5652..6782)
                     /locus_tag="PLX22_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19395.1"
                     /translation="MRTINEIQYLVYPHKNINDEIVKRFYSTAIPHKIPYKNEDDLVL
                     IVNELKDSCIDSKDVIIIKPFLGRFYQVCPGSPNMICCRYRVINTCFNCLYNCTYCYM
                     HIYLNSYGIIQFNNTDRLFAELNNFVTTCSSSMVYRIGTGEFTDSLMMDEITNLGRQL
                     IQFFSVHDTIFFELKTKSSSVDHLLDVQTKGNAVLAWSLNTSANIALYEHGTASLTER
                     INAAVKAIDAGYYIAFHFDPIIMYDNWEEDYYNIIALLKQYINPQRVVWISLGGFRYH
                     LHFREILRNKFNHEMMTTGEMFPGRDGKYRYFKPIRVKMYHFMYKHLQELFHKTYIYL
                     CMETDDVWHQITGKYFNSAEELESDMANHLYKHFVQKKTILD"
     gene            6878..7222
                     /locus_tag="PLX22_05555"
     CDS             6878..7222
                     /locus_tag="PLX22_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOT19396.1"
                     /translation="MGCLFCDIITGKTPSNKVFENDKVYAFRDISPQAPFHVLVVHKE
                     HIKNIDELTPKNSYIINDIFLAIKKIAEQEKLDKGYRVIINNGSAAGQVIWHLHVHIL
                     SGKDDLGPMLVQ"
     gene            complement(7206..8474)
                     /locus_tag="PLX22_05560"
     CDS             complement(7206..8474)
                     /locus_tag="PLX22_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT19397.1"
                     /translation="MNIYIGTQIIILIFILFSAFFSATETALVSANVLYIQNLINKGN
                     KKAKDILYLIENPDKALTMLLIGNNIANIGATAFITFFASRALLIHDSKLFIVTIVQT
                     IFFLIFCELFPKIYSRSKSEKILFFNYWLIKIFNILFMPLISLIIFITDFVKKIININ
                     DTQLLPISSRDEIDTLFRAVEKEGIIDSQHYIYFNEILSFKDVRANEVMTPLIDIISI
                     EKNQSIKLCITLIEKTRFSRIPVFEERVDNIVGYIYYQDILRNPLIKNLQQIMHPPHF
                     VPSTKKIYDLIKEMEANNVAMVFVVNEYGAVEGLLTDEDIAEEIVGEIQTRDHPQDES
                     IKEIAPWKYSIAGNLDIEFFQRRFGIKIEKHDFETVAGFLLYQMGYIPKPGEKIRYMD
                     ITFQIERATDRAIERVIVTIPKRKTTIAQA"
     gene            complement(8474..9727)
                     /locus_tag="PLX22_05565"
     CDS             complement(8474..9727)
                     /locus_tag="PLX22_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT19398.1"
                     /translation="MTLSIHLQFVIILVCLFFSAFFSGSETVLFSFKKSELIRFSFSE
                     NKLENQIAKFLSYPHNILITILTGNLFVNILISSLSTSFLLTVWPQYGHIIAILFVTP
                     VIILFVEILPKIVSLYSYKTIVYYIMPAILFFHKVLLPVRYVLLFISNTFINVFKLRI
                     DSSFDISEDDLSMAIYYGEKFGFLSQDEKQFIHNLLRFTKKTAENAMIHRSKTIFIPY
                     NATISEVISIVKETGIVRAPVYKNNLDTIVGLIDSRDIVSYATGFKKGTSIKKIIYPI
                     YHYPASRALGELLEEFLRNKIQIAVIVDEYGGTLGIVTLKRIIAELFGRGYTLWEDTP
                     KPEIKKEKGAVIIPGDMQIDEFNMMFNVSIESSESETIGGYIIEKLGYLPKRRASIMI
                     DDYIVTVRYVAKNRIQSIEVKRAES"
     gene            complement(9751..10155)
                     /locus_tag="PLX22_05570"
     CDS             complement(9751..10155)
                     /locus_tag="PLX22_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT19399.1"
                     /translation="MILFFRRKKNIKQVDMAKALNVSPSYLCKIERGVQEPTEEFKRM
                     CARYLNISVSKLFPERVKNSKDVINDIVTAKNKIWNIRMAKGIKQYELAKLLHCSPSY
                     LSKVEKGLQEPTEEFKKVCAKVLKVKEIELFT"
     gene            complement(10198..11064)
                     /locus_tag="PLX22_05575"
     CDS             complement(10198..11064)
                     /locus_tag="PLX22_05575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19400.1"
                     /translation="MTKSIHLSVTIAIIVVSLLSVSFQLPVQNGTIHSTFGEPRSDHF
                     HNGVDISSIDKKVYPVKDGKLIFLWDKSLFPLEQYPGVGNYCILEHNKQEKSLYVHLD
                     NGISMSDNYTAGDSFAIMGNTGSSYGTHLHFGIIKNGRISINPFVVLPHVDDVKTPVI
                     EAIYIKVEDKYTNIHEGSTIRLTKHHPIAIQCFDAVKKNERCGIYKLKVIVNNDIVAD
                     YKFDSIVLNDKNKLTIDGFAFENLYDAKGNYLVWSINYKQGINTINVIAEDFAGNQTS
                     KSISFMVVLDFK"
     gene            complement(11075..>13276)
                     /locus_tag="PLX22_05580"
     CDS             complement(11075..>13276)
                     /locus_tag="PLX22_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT19401.1"
                     /translation="GIPLICCFLIILYTSVVYIEWLHDKQNALDKLFKYKQLIDRTEE
                     LKGGYSYTYSEIDVSAKVVDIPTRIYDRNNEIIGEFFEQKREIVPYSYFPKWLIKAVI
                     ASEDRNFYNHKGVNYKGIARAFITNIIHFRVVQGGSTITQQLAKVLFTDMERSIKRKI
                     YEIFCAFEIEKHYDKQDILSMYLNYIYFGNGAYGVEAASKMFFGKSIVDCNETECAMI
                     VATISNPLIYSPLNNLDLSLKKTKRIMQSMIDAGFISSDQAQLQYRNFIKQWEVKFSK
                     DTIAESSLIGSFIFSLYRINRAPFFNEYIRRILVNKFSDEVVKKGGLTVYTTIDAKKQ
                     DIAITALRKGIQLQREYHEKIAKALKNPDRALMEMEKANNIEGAFVSLNPFTGEIISY
                     AGGYNFSYQNQLDHVQQIRRQPGSSFKPIIYVAAIQDKVITPSSVFDDIPMTFKGGYA
                     PKNYDGFYSGKIIIREALKKSVNIVAVQVLEKEGYSRVMSIIQKGLNLTNSELDSRFY
                     KTLSLALGTYELSPLENAVLHSVIVNGGDFITPYGIRYVKDYNGNIVWNNEEEVLLQM
                     QEKRKEFGKIIDPSACAIVVSMLKGVFEEGGTASWILKNRSIPVEIAGKTGTSSNFVD
                     AWFIGYTPDSVTAVWIGNKAGAISLGKGRSGGVLAAPVWSDYIFAVYKDAENKSFAIP
                     ADGITKQTICLDTGLVPRGEGLCPRIARDELFYTGTEPGEYCTMHTVPVLK"
BASE COUNT         4298 a         2443 c         1901 g         4534 t
ORIGIN      
        1 gttacatgaa ttaatcaatt taaatggtga tatttcagtt caatgttttt gtttatgatt
       61 gattgctgct ttttatgatg agtggtttca attattttta tcattctttc ttttaaattg
      121 atgatacctt caccagttaa acatgaacca ataattgaat cagggtataa cagccgtaca
      181 tgatgtataa catcgggcga aacaatatcg catttattaa aaaataaaat attgggttta
      241 ttattagaat ctaacaattg cagttcatgg gcaacaactg ctactgccct atcaatattt
      301 atatccaatc caacaacatg aataataaaa tcagaatatt ttatttcttc aagagttgat
      361 ttaaatgatt caataagatg tgacgggaga ttgttaataa atccaacggt atctgtaagt
      421 aatacattgt gttggttatc taaataaaca cttcttgtat atggatcaag ggtagcaaat
      481 agtctatcct ctacaaacaa atcatcttta gccaatatat ttaccaatgt ggatttacct
      541 gcatttgtat aacctactac agcaccaatt atttcattat gtaaacgttg ctgcctttgt
      601 agatctcttc tatgtgatat gtgaattaat ttttttttca gtgttgttat tcttcgtaat
      661 atatgtctcc tgtcagtttc caacatcttt tctccgggtc ctctggtgcc tattccgcca
      721 cctaaacggg ataaaactcc acccaaaccc ttgagacgcg ggaggatata gttaagttgt
      781 gcaagctcta cctgaatttt tgcttctgtt gttcttgctc gtcttgcaaa aatgtctaaa
      841 ataacctcag ttctacctat aactctgcag tgtaaataat tttctaaatt gcgtatttgt
      901 accggcctga ttgtatttag atcaaaaaca acaagtttta tagattgttc tgcaataagg
      961 ttctttattt cagtaagttt acccattcca atcaagtatg ctggatcaaa agccatgcgc
     1021 ttaacaatat agcgcgttac tacaactgca cctgctgtat gcaccaattg tgttaattca
     1081 tccagtgaat gtgatatttc tgattcatca gcttcaggta gcaacacacc aataaggatt
     1141 gctctttctt tttctgaaat aggaatccta tttactgatg catccatgca ttattacctt
     1201 tgaaccaact gatatggcat tttttttaaa ccaaccttta ttgacttcca atgcataacg
     1261 agcagggtat tttgatgagt attctggaaa agtctggaat ggattcatat catgcatatc
     1321 attgataata ccattggcat caataaaagc aatcgataag ggaatagcgg tatctttcat
     1381 ccagaaagtt cggtaagttt catctgcaaa aacaaacagc ataccgcaat catcacataa
     1441 tgcagttcta ttcatcaatc cagttgcatg ctcttcatcc gtgagagcta tctcaacatt
     1501 gcattgtatt ttttcattta atgtattaac tattgttatg atacaatgcc gtttatcatt
     1561 agcatatata tatccactat gattggtaag gaaagcataa ccataaatca ttgaacagaa
     1621 aactactata ctactcatat atattaatat aatttttttt ctcatttttt caataagtca
     1681 tttaaattga tgtcgctata attaaaactc attaatatat tcaatacgta taatcctgtt
     1741 tggattttca tgcttctgga agagtacatc aaattcaatg cccagtcgct gcatgtatga
     1801 atatcgcatg cccacattat aaagccaatc ctcgggatta ctgaaattat aataaacacg
     1861 ttccatctct gctttgaaaa caaacatatc acccagtgga atatcaaacg ataaaaaata
     1921 tgaggtatct tcgggaacat aatcgggctg tgttggcatt cgcataccaa aatgtatatg
     1981 ctgttcatca tcaaaaagat atataggttt tgttattaca aaataagggc cataaattac
     2041 ctggtcaaga tcatttttgc tattatatgt tttaccctct tcaccaatgt agaatgaatc
     2101 atagccaatg gcaaatgaaa tggggaacat ctcccatcca tcggtaaact ttatcttgcc
     2161 tacaacgcct ggaacatttg gatcaggatc atctttccct attgcacttt gacaatcaaa
     2221 tgaagcacca agataaaaat tattgtgcag tccaagaaat gcttttagtt caactccacc
     2281 gttgtcataa cctaaaaatg ataattgata ggtgccacgt gcaatagtat aggcagtagg
     2341 agtatctatt accgttgtat tgcgaacgta atattgagca tatgcaacat caaagcataa
     2401 aatacacatt ataatcatta ttaatttttg cattattgta ataccatcca ggttattttg
     2461 aaataggata tttaattata tatttatatt cggctaatat cagcacaatt tgagtatttc
     2521 attttgtatc tggaagagtt tttctatacc ctttttggcg atagttatca tggaatcaag
     2581 ttcatggaaa gcatatgaac gtttttcacc agcaccttga atttcaataa tctggtaatt
     2641 gctgtccata acaagattca tgtctacgtc acactgtgaa tcctctgcat agtctaaatc
     2701 taacagtaag ctatcaccta tatatccagc tgatattgca gcaaccatgg caattacagg
     2761 gttaacatct attacattgc gatgtatcaa tttctggata gctatctgta aagcaatgta
     2821 tgcaccggta atggctgcag tacgtgtacc accatcagcc tgtagaacat cacagtcaac
     2881 aaccaatgca atattttgta atgcagttaa atcaaccact gttcgcaagc agcgtcctat
     2941 cagacgctgt atctcaaccg atcttccatc aggttttatc ctatttcgtt ctgttcttgg
     3001 aacagtagca tagggaagca atgtatactc tgctgttaac cattatactc tgctgttaac
     3061 catcccgtac ctttatccag tgcatgctgg ggcacctggt ttgagcatga tacagcacat
     3121 ataacctgag tgttcccgca ttgatacagt actgaaccca gggcattggg gcaatagtct
     3181 gtataaactg tcacttcacg catttcattg tactgtcttt tgttctttct caagtaactc
     3241 attttgcctc ttcaactctt taagttcttc cagatccttt atttttttat caacattttt
     3301 gtggtatgga tcataatcac tggctttttt aaactcttca agggcaccat ctatatcatc
     3361 cgttataaag tatttgatac cacgttgata atgtgcctca gcttttagct tatcctctat
     3421 tgtaattacc ctgggaataa gtttaaatgt tactgtgaac gagtagattg gtccaaatac
     3481 tccagcatcc tgatatgacg catctatgac aaatgcaaca tttttaattg catagttaaa
     3541 acccatacca aaagtatatg ttccagcatc attaaaccta tagcctgttc tgaaaaataa
     3601 gatatcaaga taatttaact caataccggt atttactcta aagctttcat taaatccgga
     3661 ggtgaaatca taaagatccg atgaattaat ggcattttct ataaagtcaa cattaatccc
     3721 aaaaaaatgc gacattttat aggaaacccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn aaaacagatg ttgaaaatcc aaaaaaacca ccgtaaagag gtaaagctaa
     3901 tgatatattt tcaaaccgtg aatccagtat caattcctga tgcattattg acattacagg
     3961 aaattttaat gtagcaagac ctgcaggatt gtaatatatt gcgtttatat cattaacctc
     4021 tgcagtaaaa gcttcgccca ttcctaaagg acggctccca accccaattt ccagaaaatc
     4081 tgcacctgaa gttcctcctg aaccagcagc aaagatagta tttattgcat taatgatgat
     4141 aaataatata agtaacaata gggaaagtgt atatagtata tattttttat aatttatcat
     4201 ataattgaat aattacattt ttacgaatat atatcactca tgctttcgta ctattgttga
     4261 tgcataatga aattataaaa gatattccaa caactaataa ctaaaaacta tagagtatat
     4321 attcgtattt attataataa gtacatgact aattataatt tatgcaataa aaaaatagtc
     4381 aagcttagat attttaaata tccaataatg cattgaatac aaataagcaa agtgaagaat
     4441 tgctttttta tttacgatta atatttttta agctttttaa ataagcatat ataaatttat
     4501 taatttctcc atccataaca tattcaaccg aacccgtttc ttcccctgtt ctatgatcct
     4561 taaccattgt gtatggatga aaaacatatg aacgtatttg atttccccat gcgatatcct
     4621 tcttttcacc aaatttttcc attttttctt tctctaattc ctgtcttttc cgttcgtaca
     4681 atcttgattt taaaattttc attgcatatg ctttattctt atgttgtgaa cgttcattct
     4741 gacattgagc aacaattccg gttggaatat gagtaatacg cactgctgaa tcagttcggt
     4801 taacatgttg accgccagat cctgatgctc ggtaagtatc tatccgcaaa tcagattctt
     4861 ttatatcaat atcaatattg tctggtgcct cagggataac atctaccgat gcaaatgacg
     4921 tatgtcgcct ctgattacta tcaaatgggg aaatacgaac caacctgtgg ataccgcttt
     4981 ccccttttaa aaaaccataa gcatactggc ctttaataag caaggttgct tctttaatac
     5041 ctgcttcaat atcaggcata taatccagca cctcaacctc aaaatcatta tcttcagccc
     5101 atcgcatgta catgcgcatc aacatacttg cccaatcctg agattcggtc ccacctgcac
     5161 ccggatgtat cgtcataatg gcatttttat catcatctgc acctgataac aattcatatg
     5221 tttccagttg ctcaaatcgg ttgcgaaatt gctcaatctt gctgccaata tccttcatta
     5281 cgtcattatc tttctcatca atggccattt ccagcaaaac aattgaatct tctacttctt
     5341 gttttaaggc aaccaatggt tcgatagttc ttttaaattg agttatctgc tgttgaatct
     5401 gtgtgcagtg cgcttgatcc tgccaaaaat tttctttaaa cgtttcttct attagcaggc
     5461 ttaattgctt ttttttatca tctatattca gtgtagtata aagttcacca atatcattct
     5521 ttatttggga caattcccgt aacagatctt ttgtctcaat tatttccaat gtatgtactc
     5581 ccatttaata attacataaa atacaatgac aatctttatt aattacaata aatatgcaat
     5641 atagcacacg gtcaatctaa aatggttttt ttttgcacaa agtgtttata taaatgatta
     5701 gccatatctg attccaattc ctcggctgaa ttaaaatatt ttcctgttat ctgatgccat
     5761 acatcatcag tttccataca tagatatata tatgtcttat gaaatagttc ctgtaaatgc
     5821 ttatacataa aatgatacat tttcactcgt ataggtttaa aataacgata tttaccatcc
     5881 cttcccggaa acatttcacc tgttgtcatc atttcatgat taaatttgtt ccgtaaaatt
     5941 tctctaaaat gtaagtgata tctaaaacct cctaacgaaa tccatacaac ccgctgtgga
     6001 ttaatatact gtttcaataa tgctattata ttgtaataat cctcttccca attatcatac
     6061 ataataatgg gatcaaaatg gaaagcaata taataacctg catcaatagc cttaacagct
     6121 gcattaatcc tttcagtcaa cgatgctgta ccatgctcat acagtgctat attagcactg
     6181 gtatttaagc tccatgctaa tactgcattg ccttttgttt gaacatcaag taaatgatca
     6241 acgctggatg attttgtctt taattcaaaa aagatagtat catgaacaga aaaaaattgt
     6301 atcaattgcc ttcccaaatt ggttatttca tccatcataa gtgaatcggt aaattccccg
     6361 gtaccaattc tatagaccat tgatgatgaa caagttgtta cgaaattatt caattcagca
     6421 aaaagtcgat cagtattatt aaactgtata ataccataac tgttcaaata gatatgcata
     6481 tagcaataag tgcaattata taaacaatta aagcaggtat taataaccct gtacctgcaa
     6541 cagatcatat ttggcgaccc aggacaaacc tgataaaatc tgcctaaaaa cggctttatg
     6601 ataataacat cctttgaatc aatacaacta tctttcaact catttacaat taatacaaga
     6661 tcatcttcgt ttttatacgg tattttatga ggtattgctg tactataaaa tcttttaaca
     6721 atttcatcat taatattttt atgaggataa acaagatact gtatttcgtt aattgttctc
     6781 attttctaaa atttttcttg aatttttttt agtttacaat ctattttatc aacacatggt
     6841 ctatagtaaa tataaatttt taatggagga aatatttatg ggatgcttat tctgcgatat
     6901 tattacaggg aaaacacctt caaacaaagt atttgaaaat gataaggttt atgcattcag
     6961 ggatataagc ccgcaagctc cctttcacgt acttgttgta cataaagaac atataaaaaa
     7021 cattgatgaa cttaccccaa aaaacagcta tatcatcaat gatattttcc ttgcaataaa
     7081 aaaaatagct gagcaggaaa aattggataa agggtatcgc gttatcatta ataatggatc
     7141 tgctgcaggt caggtaatat ggcatctgca tgtacacatt ctgtcaggta aagacgattt
     7201 gggtcctatg cttgtgcaat agtagttttg cgttttggta tagttacaat aaccctttca
     7261 attgcacgat cagttgctct ttcaatctgg aatgttatat ccatgtagcg gattttttct
     7321 cctggctttg gtatatatcc catttgatat aataaaaatc cagctaccgt ttcaaaatca
     7381 tgtttttcta tttttatacc aaatctgcgc tgaaaaaatt caatatctaa attaccggca
     7441 atactgtatt tccatggagc tatctcttta attgattcat cctgaggatg atcccttgtt
     7501 tgtatttcac caactatctc ttctgctata tcctcatcag taagcaatcc ttctacagcg
     7561 ccatattcat ttaccacaaa aaccattgca acgttatttg cttccatctc ctttattaag
     7621 tcatatattt tttttgttga agggacaaaa tgtggtggat gcattatctg ctgaagattt
     7681 tttattaatg ggtttcttaa tatatcctga tagtagatgt agcctacaat gttatcaaca
     7741 cgttcttcaa aaacaggaat tcgtgaaaat cgtgttttct caattaacgt aatacatagt
     7801 tttatcgatt gattcttctc tattgagata atatcaatca aaggagtcat aacctcattg
     7861 gcgcgcacgt ctttgaatga aagtatctca ttaaaatata tataatgttg tgaatcaata
     7921 atgccttctt tctcaacagc tcgaaacaat gtatcaattt catctcgcga acttataggc
     7981 agcagttggg tgtcgtttat gttaataatt ttttttacaa aatcagtaat aaaaataatg
     8041 agcgatatta atggcataaa caaaatgtta aaaatcttaa ttaaccaata gttgaaaaat
     8101 aaaatctttt ctgactttga acgtgaataa atctttggga aaagttcgca aaagattaaa
     8161 aagaatattg tttggacgat agttactata aataattttg aatcatgtat aagaagagct
     8221 ctgcttgcaa aaaaagtgat aaacgctgta gctccaatat tggcaatatt attacctatc
     8281 agaagcatgg ttaatgcttt atcaggattt tcgattaagt aaagaatatc ctttgctttt
     8341 ttattgcctt tgttgataag attttgaatg tataaaacat ttgcagaaac caatgctgtt
     8401 tcagtagcag aaaaaaaagc agaaaaaagt ataaatatca atattataat ttgtgtacca
     8461 atatagatat tcatcatgat tctgctcgtt tcacttcaat ggattgtata cggttttttg
     8521 caacataacg aactgtcact atataatcat caatcattat ggatgctcta cgtttaggta
     8581 agtatcctaa cttttcaata atataaccac caatcgtctc gctttctgat gattctattg
     8641 aaacattaaa catcatatta aattcatcaa tttgcatatc acccggtatt atcacagcac
     8701 ccttttcttt ttttatttca ggtttagggg tatcttccca taatgtatac ccacgcccaa
     8761 ataattcggc tataattctt ttcaaggtaa caatccccag cgtaccacca tactcatcaa
     8821 ctataactgc tatctgaatc ttattgcgca aaaattcttc taaaagttca cccagcgctc
     8881 ttgaagcagg ataatgataa attggatata tgatcttttt tattgatgta ccttttttga
     8941 aaccagttgc atatgaaaca atatctcttg aatcaattaa tcccacgatt gtatcaagat
     9001 tattcttgta tacaggggct ctcacaatac ctgtttcttt aactatgctt ataacttcag
     9061 aaattgttgc attatagggt atgaaaattg ttttgcttct atgtatcata gcattttcgg
     9121 cagttttttt tgtaaaacgt aatagattat gtataaattg cttctcatct tgtgataaaa
     9181 atccaaactt ctctccataa taaatagcca tagataaatc atcttctgaa atatcaaagg
     9241 atgaatctat tcttaatttg aatacattaa taaaagtatt tgatatgaaa agtaaaacat
     9301 atctgacagg tagtaacacc ttatggaaaa ataatatcgc aggcataata taataaacaa
     9361 tggttttata gctatagagc gatacaattt ttgggagtat ttcaacaaat aaaatgataa
     9421 caggagtaac aaaaagaatt gcaataatat gtccatactg tggccataca gttagcaaaa
     9481 atgaagttga cagggaagat attaaaatat ttacaaataa atttccagta aggattgtta
     9541 ttaaaatatt atgcggatac gataaaaatt ttgcgatttg attttctaat ttattttctg
     9601 aaaatgaaaa ccgtataagc tcgctctttt taaaagaaaa caatactgtc tctgatccgg
     9661 aaaaaaatgc tgaaaaaaac aaacacacaa gtatgataac aaattgtaag tgtatcgaga
     9721 gagtcatagg tattcattaa acctgatacg ttaagtaaat aactctattt cttttacttt
     9781 taaaactttt gcacatactt ttttaaattc ttcggtaggt tcttgcaacc ccttttcaac
     9841 ctttgataga tatgatggag aacaatgcaa taattttgct aattcatatt gtttaatacc
     9901 ttttgccatg cgaatattcc atattttatt tttggcagta acaatatcat ttattacatc
     9961 tttagaattt tttacacgtt caggaaataa ttttgataca cttatattta aatatctggc
    10021 acacatcctc ttaaattcct ctgtaggttc ctgaacccca cgttctattt tacataaata
    10081 tgatggtgaa acatttaaag ctttagccat atcaacttgc ttgatatttt ttttccttcg
    10141 aaaaaataga atcatgttcg acacaaagat acccctcctt gctatgaaag ctctttatta
    10201 tttaaaatca agcaccacca taaagctaat tgattttgag gtttgattgc cagcaaagtc
    10261 ttctgcaata acatttattg tattgatacc ctgtttataa ttaatagacc acactaaata
    10321 attacctttt gcatcatata aattctcaaa tgcaaagcca tcaattgtca acttattttt
    10381 atcatttaaa acaatagaat caaatttata atcagccact atatcattat taactatcac
    10441 ttttaattta taaattccac atcgctcatt ttttttaaca gcatcaaaac actggattgc
    10501 aataggatga tgctttgtta gccgtatagt gctgccctca tggatattgg tatatttatc
    10561 ttcaacttta atgtatatgg cttcaatgac aggtgttttg acatcgtcaa catgtggtaa
    10621 tacaacaaaa ggattaatcg aaatccttcc attttttata atcccaaaat gaagatgtgt
    10681 accatatgag ctgccagtat tgcccattat tgcaaaacta tcacctgccg tataattatc
    10741 actcatacta ataccattat ctaaatgaac atacaaagat ttttcttgct tattatgctc
    10801 aagtatgcaa taattgccga caccgggata ctgctcaagg ggaaacaatg atttatccca
    10861 caaaaaaatc aattttccat ctttaactgg ataaactttt ttatcaattg aggatatatc
    10921 aacaccatta tgaaaatggt cagatcgagg ttcaccaaat gttgaatgta tagtaccgtt
    10981 ttgtactggc aattgaaatg aaaccgacaa caatgatacg acaattatgg cgatagttac
    11041 tgataaatgg attgattttg tcatagtatc accatcactt taatacaggc actgtgtgca
    11101 tagtgcagta ttcacctggt tcagtgcccg tataaaataa ttcatccctt gcaattcgag
    11161 ggcacagccc ttctccccgt ggaactaatc cggtatcaag acatatagtt tgctttgtaa
    11221 tgccatcagc aggaattgca aacgatttat tttctgcatc tttataaaca gcaaagatat
    11281 aatcagacca aactggcgcg gctaaaacac caccgcttct tccttttcct aatgatattg
    11341 cacctgcttt attaccaatc cagactgcag tcacggaatc gggagtatac cctataaacc
    11401 atgcatcaac gaagttactg cttgtacctg ttttacctgc aatttcaacc ggtatacttc
    11461 tatttttcag tatccatgag gcagtaccac cttcttcaaa aactcccttc aacattgaga
    11521 caacaatggc acatgcagac ggatctataa ttttaccaaa ctctttccgt ttttcctgca
    11581 tttgaagcaa tacttcttct tcattattcc atacaatatt cccattataa tctttaacat
    11641 atcgtattcc atatggtgtt ataaaatcac caccattaac aataacagaa tgtaacactg
    11701 cattttccag cggagaaagt tcataggtgc ctaatgctaa tgataatgtt ttataaaatc
    11761 gtgaatccaa ttcactattg gtaagattca accctttttg aataatagac ataacccgtg
    11821 aatatccttc tttttcaagt acctgcactg caacaatatt gactgacttt tttaatgcct
    11881 cccgaattat tatcttcccg gaataaaaac catcataatt ttttggagca tacccaccct
    11941 tgaaggtcat tggaatatca tcaaaaacag atgaaggagt gattacttta tcctgtattg
    12001 ccgcaacgta aataatgggt ttaaatgatg aacctggttg ccgtcgtatc tgctgtacat
    12061 gatccaactg gttctgatat gaaaaattgt agccaccagc atatgatata atttcacccg
    12121 taaaaggatt caacgaaaca aatgctccct caatattatt ggctttctcc atttccatca
    12181 atgctctatc aggatttttt aatgctttag ctattttctc atgatattca cgttgaagtt
    12241 gaataccttt tcgcaatgct gtaatggcaa tatcctgttt tttagcatcg atagttgtat
    12301 atactgttaa accacccttt ttaacaactt catctgaaaa tttattaacc agtatacgcc
    12361 gtatatattc attaaaaaac ggcgccctat tgatacgata caggctaaag atgaaacttc
    12421 ctattaaaga agattcggcg atagtatcct ttgaaaactt gacctcccat tgcttgataa
    12481 aatttctata ttgtaactga gcctggtcgc tacttataaa tccagcatct atcatggatt
    12541 gcatgatccg tttggtcttt ttaagtgata aatcaagatt attaagtggt gagtaaatca
    12601 acgggttaga aatggttgca acaatcattg cacattctgt ttcattacaa tcaactatcg
    12661 attttccaaa aaacatcttt gatgctgctt caacaccgta ggccccatta ccaaagtaaa
    12721 tataattaag atacatggat aagatgtcct gcttatcata gtgtttttca atttcaaatg
    12781 cacagaatat ctcatatatc tttcttttta tacttcgttc catatcagta aacaatactt
    12841 ttgcaagttg ctgggtaatg gtgctgcctc cctgtacaac ccgaaaatga atgatgttag
    12901 taataaatgc acgtgcaata cctttgtaat ttacaccttt gtgattataa aaatttctgt
    12961 cctcgcttgc aatgacagct ttaataagcc actttggaaa gtatgaataa ggaactatct
    13021 cccgtttttg ttcaaaaaac tcaccaataa tttcgttatt tctatcataa atgcgggtag
    13081 gaatatctac tacttttgcc gaaacatcta tttcgctata ggtatacgaa taacctcctt
    13141 ttagctcttc agttcggtca ataagctgtt tatatttaaa gagtttatcc agtgcgtttt
    13201 gtttatcatg cagccattct atataaacaa ccgatgtata cagaataatg agaaaacaac
    13261 atatgagtgg aatacc
//
LOCUS       DAOSOX010000053        13185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4730_length_13185_cov_6.985834, whole genome shotgun sequence.
ACCESSION   DAOSOX010000053 DAOSOX010000000
VERSION     DAOSOX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4730_length_13185_cov_6.985834"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..556
                     /locus_tag="PLX22_05585"
     CDS             278..556
                     /locus_tag="PLX22_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19402.1"
                     /translation="MPKEKFFCANCMNCIVIRQYESDPERFVLRVRCTKKQWTKRSGE
                     EKLYKYFTVSRRTVDNCQYYNEAGDLYPYIKNLRRDLPIKDEIYSTRE"
     gene            560..1879
                     /gene="rsxC"
                     /locus_tag="PLX22_05590"
     CDS             560..1879
                     /gene="rsxC"
                     /locus_tag="PLX22_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT19403.1"
                     /translation="MIVHSFHGGIHPPDNKHFTNRIPFKYLSIPQKSFIPLQQHIGKP
                     ATPVVEAGQLVEEGQLIGQANGLISANVHATVPGKVVEIAEYHTPYSEHGLCVVIESD
                     GSFNTSGKVRQKNDFLSLDKEALIEKVQSAGIVGLGGAAFPTAVKLNPPANRTIDTLI
                     INGSECEPYLTVDDMLMQTHPEEIIEGTQIAMKILGINKAYIGIEKNKPLAIKALQTA
                     AANVETHEITIIPLNMRYPQGAEKQLIYAITGREVPSGGLPMDVHVVVQNVGTIYAIR
                     EAVLFDKPLIERYITVTGKIVRKPGNYKVRFGMRVSDILEECGGLVENPSKIIMGGPM
                     CGLALNHMDIPVVKGTSGILFLAKNETKVTPFRSCIRCGRCVSVCPMNLIPCDLGNAA
                     EKQRYDIAEKLHPFDCIMCGSCSYICPSKRPLSHFIKVVQTTLSAKR"
     gene            1916..>2325
                     /locus_tag="PLX22_05595"
     CDS             1916..>2325
                     /locus_tag="PLX22_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT19404.1"
                     /translation="MEIKDADKKRLLEDLVLSSPPHIKDTQSVPLVMWLVVLSLMPAT
                     IFSCYLYGLYVALVIVTSIISAIVSEIIMQYLLKRPITVNDGSAVITGLLLAMNLPPH
                     VPLWIPAIGSAFAIIIVKQLFGGLGYNIFNPALAG"
     assembly_gap    2326..2425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2426..3153
                     /locus_tag="PLX22_05600"
     CDS             <2426..3153
                     /locus_tag="PLX22_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT19405.1"
                     /translation="KQLFGGLGYNIFNPALAGRAFLLASWPVEMTTLWHRFPNGYVIA
                     QNPIFNAAMPQQLIDSISGATPLGALKEAPKLLQELSLQAQPLYDFLFSNAMIKSLFI
                     GNIGGCIGETSAILLLLGALLLLWKKVITWHIPLSYIATVAACMGIYYYFTGVPNLHY
                     AILFHVLSGGLILGAFFMATDMVTSPVTKTGMIIFGIGCGILASIIRLWGGYPEGVSY
                     SILIMNGITPLIDKLTRPKVFGA"
     gene            3202..3882
                     /locus_tag="PLX22_05605"
     CDS             3202..3882
                     /locus_tag="PLX22_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT19406.1"
                     /translation="MNTFQMIKAILVLTIIGFIAAFSLSFMNKLTSEPIAKQDQEKKL
                     LSLSLVLPSYTIQKENTVTIDGQPFTYWEASKEENSVIKKAYAFITEEKGYGGTIQSI
                     VGIDDTGKILGFTILSQSETPGLGARCTEITSNETFFDHFFKSNITNTEAIQWFQVQF
                     QGLDTNKPINILKKGDWNNTIKDALLQQNAITAITGATITTKAVTLSIEKGAQKLKKA
                     LTLHEVKQ"
     gene            3879..4490
                     /locus_tag="PLX22_05610"
     CDS             3879..4490
                     /locus_tag="PLX22_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOT19407.1"
                     /translation="MNNYFKEIFKGVWEKNPTFVLGIGMCPTLAVSTSVSNAIWMGIA
                     TTFVLLCSNILISTIREYVPSHVRIPIFITIIASFVIIVELTLKAYQPSVYKALGIFI
                     PLIVVNCIILGRAEEFASKNTILHSILDAIGMGLGFTLNLTVLSAIREILGANKLLGY
                     QLIPGMKPALLMVLAPGAFFTLGLLLWGMNVIKARAALFKRTE"
     gene            4510..5112
                     /locus_tag="PLX22_05615"
     CDS             4510..5112
                     /locus_tag="PLX22_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOT19408.1"
                     /translation="MEIKILFTILISTIFINNYVLSRILGLCPYLGVSKKLDSAIGMG
                     LAVIFVMTMASFFTFIIYKLLLLPYHVEYLRTIAFIIVIATLVQFVEMIIAKISPSLY
                     QALGIFLPLITTNCAVLGVAVLNIDSGYITQQYGLLYSVTQGFGGGVGFTLALIIMAG
                     IRERLELAFIPENLKGLPITFITAGLMAISFLGFSGMKLF"
     gene            5187..5828
                     /gene="thyX"
                     /locus_tag="PLX22_05620"
     CDS             5187..5828
                     /gene="thyX"
                     /locus_tag="PLX22_05620"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT19409.1"
                     /translation="MEIYSSTVTLLAITPNAEKLIEEAGRTCYLSMDRITEGSEANFI
                     RRCIINGHHSILEHASASFRVLGASRAFTHQMVRHRLASFSQQSQRYVNEEEFNYIIP
                     PSIADNGESKELYMQFMDNARKTYKKLRSMHIKKEDARFVLPNALESQIVISANFREW
                     RHVFSLRLEKAAQWEIRNVCIQMLAILQKEAPSVFGDFIIDEATCTARSNIVK"
     gene            5890..6219
                     /locus_tag="PLX22_05625"
     CDS             5890..6219
                     /locus_tag="PLX22_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19410.1"
                     /translation="MSISSSPSVYTSDNNIQISNAPFTDGNSPTTPTLVMFIALPAFT
                     LIYGVSTWDWGDQKSFHVNRKGFFGENTNAGGANKTGHAFSHYLAFRALYNWKQFAFL
                     LMDSLQK"
     gene            complement(6247..8124)
                     /locus_tag="PLX22_05630"
     CDS             complement(6247..8124)
                     /locus_tag="PLX22_05630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT19411.1"
                     /translation="MQLKKMHNDIYQSKKINFNKLYEIIKPGSRLFLSSGPAMPICTV
                     EQMIKSDATSLQDLEIIQLITLGDYLKTGEHQYKYRLKTFNTGESISKDIEQGKADFV
                     PAHLVAIPFIFSSRAIGVDVAIIQTSPPDHRGFMSLGIAIDIANIVIENAKIVIAEVN
                     PHVPITMGDTAIHIDAIDYILESDVPLIERELKQYDPMMDKIGWHVANLIDDGSVVAL
                     HVGRQFDAIAQHLHARRELKVYSHVVSDWIIDLIESKALKHGRGINNLAPVTLSYCYG
                     TRMLYDYVDHNPNFEFLPLMRLTYPTILQRIPKLVSVMNVKKIDISGESVVFYSGDNL
                     LSGYESKLNFAVGAAFSRGGKAIVALRSVDQHGDSNIVIRHTETEQVRSMLGVTRYVV
                     TEYGVANLFGKSIRERALALIDIAHPNHRIKILNQAKEAGLVYPDQIYVIKNIINYPI
                     TLETMKTFKDGLVVKFRPIKPSDEDMMRRLFYQFSDESKYLRYFARIRTMPHKQMQSY
                     VNIDYDKILSIVGLIEHAGTERIIAEARYAYDENDKSYEMAFIVDEEFQGKGIATFLV
                     RYLMKIAKERKIERVTANVLPQNDKMIKVFEKCNVPYTQRYDDGVMHFDFHLKKAEIV
                     L"
     gene            8410..8709
                     /locus_tag="PLX22_05635"
     CDS             8410..8709
                     /locus_tag="PLX22_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19412.1"
                     /translation="MEINLEKKGNTIDIKVKGDIEMMTIKEFKEKLLSIGEENDIDIT
                     LDLSEVDYIDSSGVGVLISLNKIQKKKGKKLELAKVSPKVLNVLKLSSLSEVFNL"
     gene            8746..9240
                     /gene="ispF"
                     /locus_tag="PLX22_05640"
     CDS             8746..9240
                     /gene="ispF"
                     /locus_tag="PLX22_05640"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT19413.1"
                     /translation="MNYQNYRSGIGFDAHRLIEGRKLILGGVHIPFDFGLSGHSDADV
                     VTHAIIDALLGASSMGDIGRLYPDTSQQFKDTYSIDLLHNTYLLLLKNNITIINIDSI
                     ILCQKPKIVPYIEEMKNKLSMACGNMPVANISMKGKTTEGMGFTGTGEGIAALATCLI
                     YCNK"
     gene            9684..10901
                     /gene="tyrS"
                     /locus_tag="PLX22_05645"
     CDS             9684..10901
                     /gene="tyrS"
                     /locus_tag="PLX22_05645"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT19414.1"
                     /translation="MIKNDIELLKRGVEEIIPMDEFEKKIEHSEKENKPLIIKAGFDP
                     TAPDIHLGHTVLLRKMRHFQKMGHTVIFLIGDFTAMIGDPSGKSQTRKRLTREEVLKN
                     AETYKKQVFKILDPEKTIIDFNSRWCMPMTFADVLDLASRYNVARMLERDDFAKRYKE
                     GIPISILEFLYPLIQGYDSVALKADIELGGTDQKFNLLVGRDLQREYGQEPQVILTLP
                     LLVGLDGQQKMSKSLNNYIGIDEDPQQIFGKVMSISDELMFLYFELVTDVPELEIDGF
                     KKGIQDGSVHPKDVKVRLAKEICAQFYNEKIAEEAKLEFDKIFVQKDLPSDMPEYRIP
                     ESEKKNNTIWIVKLLVLSGMAKTNGEARRLVEGGGVYIDGNKVENPDAEILCDNPFII
                     KVGKRRFVKIILS"
     gene            10949..11512
                     /locus_tag="PLX22_05650"
     CDS             10949..11512
                     /locus_tag="PLX22_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT19415.1"
                     /translation="MSEYSDNELIDRVIAGDTNAFTDIVKRYQNKIFRYIYTRVYNYD
                     EACDMTQDVFVITMESLKTFRRESQFSTWLFSIAVNYCKNYRRKHRHKIYSINNADDE
                     MEFQIPDLRQNQEEMAITNESMQILLEELHKLPDDYRDILILRDIEGESYTTIAQMLQ
                     LTLANVKVRIHRGREMLKSRLEKRGLI"
     gene            11509..12165
                     /locus_tag="PLX22_05655"
     CDS             11509..12165
                     /locus_tag="PLX22_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT19416.1"
                     /translation="MNHIPIYKLSEFYDDILDAHDKEEIKEHLAQCPECQKHFEQLHT
                     MVNLLSCLQTISLKRESFALDTIQKIKKHHAHKRYYAAMRGSFIAAIILLAIITFLPE
                     SIKHDTSFENIAKISNSQNDQLKLDCIIPVTMDFNTALLLLQQNNARILHSSESYIIA
                     ESDVNSFSSIRQMINGSDSYRKNILSDVAVNHTNKYNKHYPQLTHANKKKYIFRLQLR
                     "
     gene            complement(12266..12805)
                     /locus_tag="PLX22_05660"
     CDS             complement(12266..12805)
                     /locus_tag="PLX22_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19417.1"
                     /translation="MAIQRKQYLIDRPIQITISLKFLIIPLLILVIFAGILLSFVLTN
                     GDYIAQIAGNQDSLIEMFITTPTLMDTNNEIVITGEKTFRENLGLLQAIKVNNYTLLY
                     LAIGLTILQAIGLFVYGIYITHKVAGPIYVMRHYCKEIQNGNTPQFRKLRGGDLLVDF
                     YNDFITAINTLQDKDASKS"
BASE COUNT         4216 a         2315 c         2376 g         4178 t
ORIGIN      
        1 tgatttgcat gcattggctg attatagcaa gcaacactct cactgtcggc gttaactttt
       61 cttcaggtat ttcaggtatt tgtatcttta tgtccatggg gacatgataa tagtacccat
      121 taaaaattaa tagtcttttt ttgtatttac ctacgttttt tgagcagtta cataattttt
      181 tacatataaa ctaaaaaaat aattgatttt gttttaatct catagcgata gtctttttta
      241 cattgttttc ttaaatatta aattatagga gagcgcaatg cctaaagaaa aatttttttg
      301 cgctaactgt atgaattgta ttgttatcag gcaatatgaa tctgatcctg agcgttttgt
      361 actgcgggtg cgctgtacta agaaacaatg gaccaaacgt tcaggggagg aaaagttata
      421 caaatatttt acagtatcca ggcgcaccgt tgataattgt caatattata atgaagctgg
      481 cgatctttat ccctatatca aaaatttaag gagggatctc cccattaaag atgaaatcta
      541 ttctacaagg gaataatcta tgatagtcca cagtttccat ggaggaattc atcctccaga
      601 taataaacat ttcaccaacc ggattccatt taaatatctt tcaattccac aaaaaagctt
      661 tataccccta caacaacata ttggtaaacc agcaacaccc gttgttgaag caggtcaact
      721 tgttgaagaa gggcagctca ttggtcaggc aaatggactt ataagtgcta acgttcatgc
      781 aactgtacca ggcaaggttg ttgagattgc tgaataccac actccttaca gcgagcatgg
      841 gctatgtgtt gttattgaat cagatggatc gtttaataca tcaggcaaag tacgccaaaa
      901 aaatgatttc ctgagccttg acaaagaagc actgattgaa aaagttcaat ctgctggcat
      961 tgttggatta ggaggcgctg cattccccac agcagttaaa ttaaatccac cagctaaccg
     1021 tactattgat accttaatta ttaatggttc tgaatgcgag ccatacctta ctgtagacga
     1081 tatgcttatg caaacccatc ctgaagaaat tattgaagga actcagattg caatgaaaat
     1141 cctgggtata aacaaggcat atataggtat tgaaaaaaat aagccgctgg caattaaagc
     1201 attacaaact gcagcagcaa acgtagaaac tcatgagatt actataatac cattaaatat
     1261 gcgatatccg cagggtgctg aaaaacagct catctatgct ataacagggc gtgaagtgcc
     1321 atcaggcggg ttacctatgg atgtccatgt tgttgtacaa aatgttggaa caatttatgc
     1381 cattcgagaa gcagttcttt ttgataaacc attaatagaa cggtatataa cggtaacggg
     1441 aaaaattgta cgtaaacccg gcaactataa agtacgattt ggtatgcgtg tcagtgatat
     1501 tctggaagaa tgcggtggac ttgttgaaaa tccatccaag attattatgg gcgggccaat
     1561 gtgtggactt gctctgaacc acatggatat acctgttgtt aagggaacat ccggaattct
     1621 ttttcttgca aaaaatgaaa ctaaggttac accattcaga tcatgtatcc gatgtggacg
     1681 ttgtgtttca gtatgtccta tgaatcttat accctgtgat ttaggaaatg cagccgagaa
     1741 acaacgctat gatattgccg aaaagcttca tccatttgat tgcattatgt gtggttcatg
     1801 ttcatatata tgtccgtcaa aacggccgtt atcacacttt atcaaggtag tacaaacgac
     1861 actgagtgca aaacgataat tgaggaaaca tattttcact atagaaggat cacctatgga
     1921 aataaaagat gctgataaaa aacgcctatt agaagattta gtgttatcaa gtccccctca
     1981 catcaaggac actcagtcag taccacttgt gatgtggctg gtagtgcttt cgcttatgcc
     2041 tgcaacaata ttttcatgct atttatatgg actctatgtt gcattagtta ttgtaacatc
     2101 cataatttca gcaatagttt cagaaattat aatgcaatat cttttaaaaa ggccaattac
     2161 tgtcaatgat ggttcggcag ttattactgg actacttttg gcaatgaacc tgccaccaca
     2221 tgtgccgcta tggattcctg ctattggttc agcttttgcg ataatcattg tcaaacagct
     2281 atttggcgga ttgggataca acatatttaa tccagcactg gcaggnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnntcaaa cagctatttg gcggattggg atacaacata
     2461 tttaatccag cactggcagg acgagcattt ttacttgctt catggccggt ggagatgaca
     2521 acactgtggc acagatttcc caatggttat gtcatcgcgc aaaatccaat ctttaatgct
     2581 gcaatgcccc agcaactaat agatagtatc agtggcgcta caccgctggg tgcattaaaa
     2641 gaagctccta aacttttaca ggaactatcg ctacaggcgc aacctcttta tgatttcctt
     2701 ttttcaaacg ccatgataaa gtcattgttt attggtaata ttggcggctg cataggcgag
     2761 acatcggcta tccttttatt gttaggtgca ttgctactat tatggaagaa agttattaca
     2821 tggcatatcc cgctatccta tattgcaacc gtagctgctt gtatggggat atactattac
     2881 tttacaggtg ttccaaacct ccattatgct atactcttcc atgtattatc aggagggctt
     2941 atactgggag catttttcat ggcaaccgat atggttacct cgcctgttac taaaacaggg
     3001 atgattattt ttggtattgg ctgtggaata ctggcttcaa ttatacggct gtggggtggt
     3061 tatcctgaag gcgtttcata ttcaatatta attatgaatg gcattacacc cttaattgat
     3121 aaattaacca gacctaaagt atttggagca tgataaggac atagttatga tagataatca
     3181 tttactagtg gagaactatt gatgaacacc tttcaaatga taaaagcaat attggtatta
     3241 acaattatag ggtttattgc tgctttttcg ctttcgttta tgaataagct taccagtgaa
     3301 cccatagcaa agcaagatca agagaaaaaa ttgctttccc tttcgttagt attaccatcc
     3361 tatactattc aaaaagaaaa cactgttact attgatggtc aaccttttac ctactgggaa
     3421 gcatcaaagg aagaaaacag cgtaataaaa aaagcatatg cattcattac tgaagaaaaa
     3481 ggttatggcg gcacaattca atcaattgtt gggattgatg atacaggaaa aattttaggt
     3541 tttacaattt tgtcccagag tgaaactccg gggcttggtg ctcgctgtac tgaaatcaca
     3601 tcaaatgaaa cattttttga tcattttttt aaaagcaaca tcacaaacac agaagctatc
     3661 caatggttcc aggttcaatt tcaggggctt gacaccaata agccaatcaa tattttgaaa
     3721 aaaggagatt ggaataatac aataaaagat gctctgttac agcaaaacgc tataacagca
     3781 attaccggtg caactattac taccaaagca gtaaccctga gtattgaaaa aggtgcgcaa
     3841 aagcttaaaa aagcattaac tctgcacgag gtaaaacaat gaataactac tttaaagaaa
     3901 tattcaaagg agtatgggaa aaaaatccca catttgtatt gggtattggc atgtgtccca
     3961 cacttgcagt atcaacttct gtttcaaatg ctatctggat gggaattgct acaacattcg
     4021 ttcttctatg ctctaatata ttaatatcta ccatacgaga atatgttcct tcgcatgttc
     4081 ggattccaat atttattaca ataatcgcaa gctttgttat tatagttgaa ctgactttaa
     4141 aagcatatca accatcggta tataaagctt taggcatatt cattccatta atagttgtta
     4201 attgcataat tttagggcga gcagaagagt ttgcttcaaa aaatactata ttacattcaa
     4261 ttcttgatgc aattggaatg ggattgggct ttactctgaa tttaactgtt ctgtcagcaa
     4321 ttcgggagat tttaggggca aataaattat taggatacca attgataccc ggtatgaaac
     4381 ctgccctgtt aatggtatta gcaccaggtg cattttttac attaggttta cttttatggg
     4441 gaatgaatgt cattaaggca cgcgctgcac tttttaaacg aaccgagtaa ccagaagtga
     4501 gggacaaata tggaaattaa aatattattc accattttga taagtacaat ttttatcaat
     4561 aactatgttc tgtcacgaat attgggacta tgcccttatt taggtgtatc aaaaaaactg
     4621 gattcggcta taggtatggg gcttgcagtt atctttgtta tgaccatggc ttcttttttt
     4681 acgtttatta tatataagtt attgttactt ccttatcacg ttgaatatct ccgtacaata
     4741 gcttttatta ttgttatcgc tacgctggtt cagtttgttg aaatgataat tgcaaaaatt
     4801 tcaccgtcac tatatcaagc attaggaatc ttcttaccac ttataacaac caattgtgct
     4861 gttttaggtg ttgctgtttt gaatattgat tctggatata ttactcaaca atatggctta
     4921 ctttattcag taacacaggg ttttggtggt ggtgtgggat ttactttagc gcttattatt
     4981 atggcaggaa ttcgagagcg gcttgaactt gcgtttatac ctgaaaatct caaaggattg
     5041 cctattacct ttattaccgc cgggcttatg gcgatttcat ttcttggatt ttccggaatg
     5101 aagctctttt aatagttttt attatttttt tcatgttttg catacgtttt ttagcgtatt
     5161 tttatattaa attttgatag atagctatgg aaatttattc atcaacagta actcttctgg
     5221 caataactcc caatgctgaa aaactcatag aggaggcagg caggacatgt tatctttcca
     5281 tggataggat aaccgaaggc agcgaggcta attttatacg tcgatgtatc ataaatggac
     5341 atcactcaat actggagcac gcatcagcct cttttcgagt attgggagca tcgcgagcat
     5401 ttacccatca gatggtgcgt caccgccttg caagcttctc acagcaatca cagaggtatg
     5461 ttaatgaaga agaatttaat tatattatac caccatcaat agctgataat ggcgaatcaa
     5521 aagaactcta tatgcaattc atggataatg ctcgtaaaac gtataaaaag ttaagaagca
     5581 tgcatataaa aaaagaggat gcacgctttg ttctccccaa tgcactggaa agccagattg
     5641 tcatttcggc aaacttcagg gaatggcgcc atgttttttc attgcgtctt gaaaaggctg
     5701 cccaatggga aataagaaat gtttgcatac agatgcttgc aatattacaa aaagaagcgc
     5761 cttctgtttt tggtgatttt attatcgacg aagctacctg tacagcgcgc tcaaatattg
     5821 ttaaataaac tatttttttt tcaatatcca taaccattcc acactcattt ttaaggacat
     5881 ttaccgtgga tgagcatctc atcttcgcct tctgtttata catctgacaa caatattcag
     5941 atcagcaatg cgccctttac tgacggcaac agtccaacaa ctcctacatt ggttatgttt
     6001 attgctttac cagcatttac attaatatat ggagtgtcaa catgggattg gggcgatcag
     6061 aaaagttttc atgttaatcg caaaggtttt tttggcgaga acaccaatgc tggtggtgct
     6121 aataaaacag ggcatgcctt ttcacattat ctggctttca gggctctata taactggaag
     6181 caatttgctt ttttattgat ggattccctg caaaaatgac atgagcaaca tattgattta
     6241 ttgtatttaa agtactatct ccgctttttt taaatgaaaa tcaaaatgca taaccccatc
     6301 atcatatctc tgtgtatagg gaacattaca tttttcaaaa acctttatca tcttatcatt
     6361 ctggggcaat acgtttgcag ttactcgttc tatcttacgc tcttttgcaa tcttcatgag
     6421 gtaccttact aaaaatgtag caataccttt cccctgaaat tcctcatcca caataaatgc
     6481 catttcatac gatttgtcat tttcatcata ggcatatcgt gcttcagcaa taatacgctc
     6541 agtaccggca tgctcaataa gaccaactat cgacaaaatt ttgtcataat caatattaac
     6601 atatgactgc atttgtttgt gaggcatggt ccttatgcgt gcaaagtatc gtaaatattt
     6661 tgattcatca gagaactgat agaagagacg gcgcatcata tcctcatcag atggcttaat
     6721 aggcctgaac ttaacaacca gcccatcttt aaacgttttc atagtttcaa gtgttattgg
     6781 atagttaatg atatttttaa ttacatatat ctgatcagga tataccaatc ctgcttcttt
     6841 tgcctgattt aaaattttaa tcctgtgatt gggatgagca atatcaataa gagctaatgc
     6901 tcgctcacga atagattttc caaaaagatt agcaacacca tattcagtca caacatatcg
     6961 tgttactccc agcatggaac gaacctgctc ggtttcagta tgcctgatta caatgttact
     7021 atcgccatgc tgatcaactg aacgaagtgc aacaattgct ttgccaccac gggaaaatgc
     7081 tgccccaaca gcaaaattaa gtttgctctc atatcctgat aaaagattgt cacctgaata
     7141 aaacacaacc gattcacctg atatatcaat tttctttaca ttcatgacac ttaccaattt
     7201 gggtatgcgc tgcaaaatgg ttggataggt aaggcgcatc agaggcaaaa attcaaaatt
     7261 agggttatgg tctacataat catacaacat gcgtgttcca tagcagtagc tcaatgtaac
     7321 tggagctaaa ttattgatcc ctctgccatg cttcaatgct tttgattcga ttaaatcaat
     7381 aatccaatca gaaacaacat gactataaac tttaagctct cgcctggcat gtaaatgttg
     7441 tgcgatagca tcaaactgtc gtccaacatg gagagcaact actgaaccat catcaataag
     7501 gtttgcgaca tgccaaccta tcttgtccat cattgggtca tattgcttaa gctcacgttc
     7561 aattaatggt acatcgcttt ccagtatgta atcgattgca tcaatatgta tagctgtgtc
     7621 acccattgtt attgggacat gagggtttac ctcagcgatg actatctttg cattttctat
     7681 aacaatattt gcaatatcaa ttgctattcc taaactcata aatccacgat ggtctggtgg
     7741 tgaagtttga ataattgcca catcaacacc aattgcacgg ctggaaaaaa taaatgggat
     7801 tgcgaccaga tgagcaggta caaaatcagc tttaccctgt tcaatatctt tactgatact
     7861 ttctcctgta ttaaatgttt taagtctgta tttgtattga tgttcaccgg ttttaagata
     7921 atcacccaat gtaatgagct ggattatttc cagatcctgt aagctggttg catctgattt
     7981 aatcatttgc tcaacagtac agataggcat tgcaggacca ctgcttaaaa ataatcgaga
     8041 ccctggtttt ataatctcat atagtttatt gaaattgatt ttttttgact gataaatatc
     8101 attatgcatt ttttttaatt gcattaaatt acctcgtaat ggtagtataa ccaatagtat
     8161 attttatatt tttttcgtat tttgtcaaca atttgatgta atgcattcat gcaaagagtt
     8221 gcacggtgtg ggaaccttta ccaataacgt tttatgggct taccatttga aaaagcttta
     8281 gcaccataac tttgttctca caatggtatt aaaaggtata gttttcaatg ttcattttat
     8341 ttattgacac atttatttat gtagtattta attagtctat atagacttta ttttatagag
     8401 gagttaacca tggaaatcaa ccttgagaaa aaaggaaata caattgatat taaagttaag
     8461 ggcgacatcg aaatgatgac cattaaagag tttaaggaaa agctcctgtc aattggtgaa
     8521 gaaaatgata ttgatatcac acttgacctt tcagaggttg attatatcga ttcgtctggt
     8581 gttggagtac ttataagcct taataaaatt caaaagaaaa agggaaaaaa attagagctt
     8641 gcaaaagtta gccctaaagt acttaatgtt ttaaagctta gttcactatc tgaagtattt
     8701 aatttatgat atgataaagc ctgaacaatc tattttttat tccagatgaa ctatcaaaac
     8761 tacagaagtg gcataggttt tgacgcccac cgtctaatag aagggcgcaa gcttatttta
     8821 ggaggagtcc atatcccatt tgattttggg ctctcaggac attcagatgc tgatgtagtt
     8881 acccatgcaa ttattgatgc gttattaggt gccagcagca tgggcgatat aggaaggtta
     8941 tatcccgata ccagccagca attcaaggat acatatagta tcgatttgct gcataatacc
     9001 tacctccttc ttttaaaaaa caatataaca attattaata tagattcaat cattctatgc
     9061 caaaaaccaa aaatagtgcc ctatattgaa gaaatgaaaa ataaattatc aatggcatgc
     9121 gggaacatgc ctgttgcaaa tatatcaatg aaggggaaaa caaccgaagg catggggttt
     9181 accggtactg gagaaggaat agctgcatta gcaacctgtc ttatttactg taacaaataa
     9241 tgatacgccc ttactaataa atcaacaaat acatgttgac agacagttat ccatcaatat
     9301 tgattgttca tttggagaat ggatttttgc cattgttatt atattttcaa cggtcgttgc
     9361 agacactctt ccagatacta acagtacaat gctatgagcg caaacagcat tgtttggcga
     9421 tagttatcct gaatcatgtc ctgaatttta tttcaggatc tcatggtgaa aagagtttcc
     9481 ggatcaagcc cgaaatgaca gtggtgatga cctgtcattc tgaagcgaag tcgaagaata
     9541 tcgtcgtttt gtgaacgaat agattcttcg gtcgctactg ctccatcaga atgacaatac
     9601 tgcgataatg cttctctcgc cttacgaagg gcaggggtcg ggaatgaggt aaataattct
     9661 attttctgag gaggatatat actatgataa aaaatgatat tgaattgctg aaacgaggtg
     9721 ttgaagagat cattccgatg gatgaatttg aaaaaaaaat agagcattca gaaaaagaaa
     9781 acaaaccttt aatcataaaa gctggttttg atccaaccgc acctgatatt cacttaggcc
     9841 atactgtttt actcagaaag atgcggcact ttcagaagat ggggcatact gttatttttc
     9901 tcattggcga ttttactgca atgatcgggg acccatctgg aaaatcacaa acacgaaaac
     9961 gattaacccg tgaagaggta ttaaaaaatg cagaaaccta caaaaaacag gtttttaaaa
    10021 ttcttgatcc tgaaaaaact attattgatt ttaactcacg atggtgtatg cccatgactt
    10081 ttgctgatgt tttggatctg gcttcgcgct acaacgttgc ccgtatgtta gagcgtgatg
    10141 actttgcaaa acgctataaa gaaggcattc ctatttccat actggagttt ttatacccat
    10201 tgattcaggg ttatgattca gtagcattaa aggctgatat tgaattagga ggtaccgatc
    10261 agaaatttaa tcttttagtt ggaagagacc tccagcgtga atatggtcag gaaccgcagg
    10321 ttatcctgac attgccactt ttagtaggac tcgatggtca acagaagatg agtaaatctt
    10381 tgaataatta tattggcatc gatgaagacc ctcaacagat atttggcaag gttatgtcaa
    10441 tctcggatga gctgatgttt ttatattttg aactggttac tgatgtccca gaacttgaga
    10501 tagatggttt taaaaaaggc atccaggatg ggtcggtaca tccaaaagat gtcaaagtac
    10561 ggcttgcaaa agagatatgt gcacaatttt ataacgaaaa gattgcagag gaagcaaagt
    10621 tggaatttga taagattttt gttcaaaagg atcttccatc tgatatgccg gaatatagaa
    10681 tacctgaatc agaaaagaaa aataatacta tctggattgt aaaattgctt gttttatcag
    10741 gcatggctaa aacaaatggc gaagcacgac gacttgttga aggtggcggt gtttacattg
    10801 atggtaacaa agtggaaaat cccgatgctg aaatattgtg cgataatcct ttcattatta
    10861 aagttggcaa acggcgtttt gtaaaaataa ttctttcata actatcgtgt aaataattaa
    10921 tttttttggt aacattgtaa ccataactat gtctgaatat agtgataatg aactcattga
    10981 tagggtaatt gcaggtgaca ccaatgcatt tacggatatt gtaaagcgat accagaataa
    11041 aatattcagg tatatctata cgcgtgttta taattacgat gaagcatgtg atatgacaca
    11101 ggatgtattt gttattacca tggaatcgtt aaaaacgttt agacgcgagt cacaattttc
    11161 aacatggctt ttcagcattg ctgttaatta ctgtaaaaat tataggcgaa aacacaggca
    11221 taaaatttat tcaattaata acgcagatga tgaaatggaa tttcagattc ctgatttacg
    11281 gcaaaatcag gaggagatgg caatcaccaa tgaatcaatg caaatacttc ttgaagagct
    11341 tcataaactt cccgatgatt accgggatat acttatattg cgcgatattg aaggcgagtc
    11401 atacactacc attgcacaga tgttacagct aacattagcc aatgtaaaag taaggataca
    11461 caggggacgt gaaatgctaa aatcacgcct ggaaaaaagg gggcttatat gaatcacata
    11521 ccaatatata agttatccga gttttatgat gacatactgg atgcccatga taaagaagaa
    11581 attaaggaac atcttgcgca atgcccggaa tgccagaaac attttgaaca attgcatact
    11641 atggtaaatc ttttatcatg tttacaaact atatccctaa aacgggaaag ctttgcgctt
    11701 gataccattc agaaaataaa aaagcaccat gcccacaagc gctattatgc cgcaatgcgg
    11761 ggatcattta ttgcagctat cattttatta gcaataataa cattcctacc cgaatcaata
    11821 aaacatgata catcatttga aaacattgct aaaattagca attcacaaaa cgaccaatta
    11881 aagctggatt gcattatccc tgttaccatg gattttaata ctgccctatt gctgttacaa
    11941 caaaataatg cacgtatact tcactcatcc gaatcatata tcattgctga gtcagacgtt
    12001 aatagctttt cttcaatcag acaaatgatt aatggtagcg atagttatcg taaaaatatc
    12061 ctttcagatg ttgcagtcaa tcatactaac aagtataata aacactatcc acagcttacc
    12121 catgcaaata agaaaaaata tattttccgt ctacaattgc gttagtattt tgttttggta
    12181 tatttaaaaa aattatttta ttgcatcaac aatagcagta aataatcaac aatgcagttt
    12241 ttcaaaaatt tcatataatg aaaattcagc ttttagatgc atccttatct tgtaacgtgt
    12301 ttatagccgt tataaaatca ttataaaaat caacgagtag atcgcctcct cttaattttc
    12361 taaattgagg cgtgttacca ttttgaattt ctttacagta atgacgcata acataaattg
    12421 ggcctgccac cttatgggta atgtatatcc catatacaaa cagccctatt gcctgaagta
    12481 ttgttagccc aatggcaaga tacaataatg tataattatt tacttttata gcctgtaata
    12541 aacctaaatt ttccctgaac gttttttcac cagttataac tatttcatta ttggtatcca
    12601 tcaatgttgg tgtcgttata aacatctcta ttaaactatc ctgattacca gctatttgtg
    12661 caatataatc accgttggta agcacaaaag ataataatat ccctgcaaat atgacaagaa
    12721 ttaaaagcgg aattatcaaa aacttaagcg atattgtaat ctggataggt cggtcaatca
    12781 aatactgttt tctttgtata gccatatata acccctttag taatttgata tagtttgtgc
    12841 tgcatatggc gctattatat attggcaatg ataactcagg atacttgtaa tatatactac
    12901 ttttttttaa cctatcgatg ttttgttatt acgtcaacaa agatttatat cattaacatt
    12961 taaacttgac aattgtgtaa tacacaaata tactatataa aaatacatac aaaagggcta
    13021 tgtatatgat aaccttaagt ttaataggtt cgtttttagc atcatgtatg ctaatattta
    13081 ctattgttta cggtttatat aaaaactttc aagatagggt aatgcgctac tactcgtatt
    13141 ttggtttttg tgcctttggt atccttttta ccatgttttt aacct
//
LOCUS       DAOSOX010000054        13060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4784_length_13060_cov_8.782930, whole genome shotgun sequence.
ACCESSION   DAOSOX010000054 DAOSOX010000000
VERSION     DAOSOX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4784_length_13060_cov_8.782930"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLX22_05665"
     CDS             <1..810
                     /locus_tag="PLX22_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT19418.1"
                     /translation="LQVYNLIIRILLMKLMNRKMVITAVVSCMVIAVSTFTIYNNNRK
                     TVKPRVTSVVDAVYALGIVTPYKEYSLRVGMSTRIEKLYIWEGDRVQAGSPLVQLDGG
                     ILLRAPFSGVVATLNRKESEIVSAQESIMTIVDPATMYIRLSLDQESIINVRKKQVAE
                     ISFENLRNKNIRGVVSRIYPSEGEFVVAIDVDSFPEGVLPYMTCDVAIKVSEKNNALL
                     LPKNAVHNGAVTVIRNGRKQLVNVSTRAHDSGWVEIVQGNITPDDTVVVMR"
     gene            853..2064
                     /locus_tag="PLX22_05670"
     CDS             853..2064
                     /locus_tag="PLX22_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19419.1"
                     /translation="MKYIISRKVQSLLMLIGVTLGCGGYIIFTSVQLGFQTFMKERLI
                     ENNGHITIESREEDITRESMEGVFFKGSTVYWISGPSGRRSNEYLQSAWHWQKRLADI
                     PFVVASTRRVEAQVTCARGAFYRSVTLRGIDPFSEPKVTNIAEDIVVGNMNMLNSGNN
                     LVIVGVELLKFLGGKLNDTITVVNSNGNVYPVKVVGIYSSGDRRNDRTLILASITTVQ
                     NILESPGRITKIVVKTVNADMASTFAHELSQGTYDKVESWDEANINFLSMTRQQSFIR
                     NITIFTFILVVSFGIYNVLNMLVHQKQREIAILRSIGYTRNDTIVLFLLQGAILSVAG
                     GIIGILIGYGACSYIETIKMPGRPMHVAWDISIYMWGFMLVVISALFASFLPAKTAGS
                     LSPIEIIRRTE"
     gene            2064..2735
                     /locus_tag="PLX22_05675"
     CDS             2064..2735
                     /locus_tag="PLX22_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19420.1"
                     /translation="MVAIEARNVVKAFGDFTALNSISLTIARGEFVAITGKSGSGKST
                     LFYSLSGLDSINNGTVMMLGYAIHSLPEDEVHRIRNTKMGFVFQFHYLISELTALENI
                     LMPAKKIKMHKEKMQYAQYLLDYFDIKHCKDKLPSQMSGGERQRVAVARALIMNPDII
                     FADEPTGNLDSINSEAVMRIFKEVNKQNGTTVILITHDDEYAHMAGRQIHLKDGQIEY
                     DIYNK"
     gene            2935..4365
                     /locus_tag="PLX22_05680"
     CDS             2935..4365
                     /locus_tag="PLX22_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT19421.1"
                     /translation="MKNKNITAYIVLFMVVSVHGFAQNNDAILSYDDYVSIMIKQLPQ
                     IKNNIIQVKKAQVNVEKAKSVDDTTLVAQGQYTHQKQYTTGKNLFEPDYSETYYGYAG
                     IERIIAPTGTRLSAGFEYDASSVKGSRTIPAQAMSIDEYQPSIKATITQPLLKNAFGI
                     ADRYGKNNAEYTLQIQKLQQLEDDNSVMNYYKKLYFTWIFYKQAIDILAESMANAKNL
                     VVTVERKAKAGLAENDDLQRAYSSLYSYQADYQQYQITYRALLDELWLYINKEATPDS
                     AYLQKYFDEIVTMDIQEIPFEKTRAYAIVAKSIDSMHYTIDVANNDTLPQMDLVLSAS
                     RKNYSQNQSDALSKLPDTDYSIGLQFSYPLGNHTSQSQLQEYELSLKELEHNLNISKN
                     DYYKNIRTILQSLKGYKDLIEINTKNLYALVSQRQTERKKYEQARLDLKYLIETENSI
                     FSQQLKLLQLKVSFIDYFIDYNDLIQ"
     gene            4451..5332
                     /locus_tag="PLX22_05685"
     CDS             4451..5332
                     /locus_tag="PLX22_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT19422.1"
                     /translation="MYQIISSSFQSNDCLLDARLYLPESNQKLPIIIMAHGFGARMDF
                     GLHPFADVFANMGFAVLMFDYRGFGKSKGSQQQLVYHKYHIEDYHSAIHYVKTTPNID
                     GAKIVLWGTSYSGGHVLTVASQDTTIAGVIAHVPFVDGIATAFHLPIKNIIIGMYSGV
                     RDMVTHIVNSKPYTIPIVSPPDSFAAMNTADSYQGYMNLVPAEVTDENWCPARVCLTL
                     PLYRPTTYVKKIMCPVCIIAAEYDSLIPLSAVKKAAGNIKNIDFHILPCGHFEPYTGT
                     MFEKSITIQKRFLERLL"
     gene            complement(5349..6935)
                     /locus_tag="PLX22_05690"
     CDS             complement(5349..6935)
                     /locus_tag="PLX22_05690"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOT19423.1"
                     /translation="MNATDIITSIGGIGLLLLGIALMSESIQKLAGSRLRIILTNLTG
                     GPGKALLTGMLITTIIQASSATAIATIGFVSAGLITMSQALAVIFGANVGSTVTGWLV
                     AIFGVQYSIGNISLILVAIGSILKILYTDKKSDIGMIMAGFGLFFFGISLMQQGMSQL
                     SSIINFSNIAGEGIFDRLILFAIGFVLTILMQSSSASVAIALIALNTGTIHLFQAQVL
                     VIGTNAGKSIPILFAGIGATPDTKRIIAADFLFNIITAIILFIIIPWLSPLLAFLFTT
                     IYPMPYEIQLALFHTLFNCIGILLFTPFITPFIRFLRTFFIDKQLYLTQHLDDAIAKN
                     PPIAVEAARRAVTDIALFIIHTLKEILQDKEDDFQRRLRFAANALNETKLFMSKVKTN
                     PHESKLYNEHISILHSIDHLNSIIEACYEKQAIDTLKNSDLFKDMLSNFSNELDESEN
                     ALQQKSSLDNVEKIQELSQYLANIRKLERIEVLKKTAMGSLDSHTALEFIEAIRFIDR
                     LGYHIWRVVRHCILPGESNY"
     gene            7549..8076
                     /locus_tag="PLX22_05695"
     CDS             7549..8076
                     /locus_tag="PLX22_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19424.1"
                     /translation="MRATHLIVTITFVLMSYVSVQAKTVLDYYCLIPDEFFQCEDDVK
                     ITRNYKLNQIKYKNIKAGYIRAESERNPMEVALFKDIKSNKDVVAVNIKCGPGCMCNR
                     FALLQFDANGKAINITASIFPNENLIWKKIKRTDVDAYEFILPEVGTTIKVVHADTGE
                     ELAQIFWRDGKFVLQ"
     gene            8282..8464
                     /locus_tag="PLX22_05700"
     CDS             8282..8464
                     /locus_tag="PLX22_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19425.1"
                     /translation="MKVKKLRCEHCSLRATFDRSPRSLIGRLWRWHITWCPGWKAYSN
                     SLTEEERGELIAKYSL"
     gene            8770..10041
                     /gene="aroA"
                     /locus_tag="PLX22_05705"
     CDS             8770..10041
                     /gene="aroA"
                     /locus_tag="PLX22_05705"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT19426.1"
                     /translation="MQFKVLPSSLKGEVVIPGSKSHTIRACVCALLANGTSTIKMPLD
                     SSDTRSCLGMIQRFGATVTQHNDEWVVTGCGIPAVPDDIIDIGNSGTSLYFGIGVASL
                     VNGITVFTGDNQIRNRPAQDLLTAITELGGWAVSTRSNGKPPVIVQGPITGGHVAIRA
                     ITSQYVSSLLIATPMAKNNTIIDVPLLNEAPYVGMTLWWLNELGIEYHANGYDQFIIR
                     GGQTYKPFSKYIPADFSSATFFMVAAAITGSTVKLKGLDINDTQGDKEVVTILKQMGA
                     DIAILPDAIVITGKPLKGGEFDLNAIPDALPALSVCGCFAEGETRLVNVPQARVKETD
                     RIAVMCAELKKMGADIEELPDGLVIRQSQLKGAPVQGHHDHRVVMSLAVAGLAAQGHT
                     VIDTAQAVDVTFPNFVELMLQLGAAIEREES"
     gene            10041..10433
                     /gene="aroH"
                     /locus_tag="PLX22_05710"
     CDS             10041..10433
                     /gene="aroH"
                     /locus_tag="PLX22_05710"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT19427.1"
                     /translation="MLRGVRGAITVDENTKEEIVSRTKELLQAIVTVNDIHTDDIASA
                     IFTVTDDLNAEFPAVAARTIGWIHIPLLCSREIPIPGSLGMCVRVLLHINTNKKQDEI
                     VHVYLRDAKKLRPDIGTPEQDKYYLSHD"
     gene            10488..11264
                     /locus_tag="PLX22_05715"
     CDS             10488..11264
                     /locus_tag="PLX22_05715"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOT19428.1"
                     /translation="MKQIKYLVVSIICYGVVVSTNAILYAQAEREIELTAQEILARVD
                     RIMQYPDGILKGYIMYITPDGSSQKLQITGYIANNQFLFDVSKPARGSMQKILYRIGG
                     EDLWVYDMAAKALYHKMGVDKFDDILATNFFYTDLSNADLQSNYVATIDGSVIIKNND
                     CYKLTLDPIDKTGIYGKLTLYVVKKTYIPLRIDYHDRDKVIFKTLSITKVAEKDEKSF
                     PVRYDMLDIKKGTMTILEFNSIEDGVRVDAELFRPEKLGD"
     gene            11267..11989
                     /locus_tag="PLX22_05720"
     CDS             11267..11989
                     /locus_tag="PLX22_05720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT19429.1"
                     /translation="MAQFFVDSAGSDNSIIITGKDFYHLTTVRRASIGDAIQIIDNNG
                     MQYHATITQIDDGKIVATITEKLHYPDIHVQCTLCAALLKGKKFDLVIQKAVEIGVKQ
                     IIPVVTQHTIPDVSGKEDKKVERWQSIASEASKQCLRKEVPAVHNVMPFEDVIRLPKE
                     IGIMAHADPKAINLRNFLKEQVKHNNVMLLIGPEGGFSKKEVEIARQYDWFVLYSGAT
                     QLRAETAAIVLPAIILYEWGYE"
     gene            12002..12199
                     /gene="thiS"
                     /locus_tag="PLX22_05725"
     CDS             12002..12199
                     /gene="thiS"
                     /locus_tag="PLX22_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOT19430.1"
                     /translation="MKIRVNGIDIEFEKKTLKELLDLYRFNPSKIVVEKNGRIIDKEQ
                     YATEVLKDGDVLEIARFVGRG"
     gene            12196..12708
                     /locus_tag="PLX22_05730"
     CDS             12196..12708
                     /locus_tag="PLX22_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19431.1"
                     /translation="MNDKFHALTESLLTQEELQHCILLLFCLGDSSCDDPLLEELQIR
                     GYIVKEKDNRIITQKGKQYLSGLMQKLAQFHNAGEIIFQHYVDRELSPIKKEIHEIRM
                     QWRAVRDKRLARKAELQKMGKDKSALQKDSIYKALQKEQNALRTHLKHTEKKYHKKLA
                     NHVKNVGMLP"
     gene            12789..>13060
                     /locus_tag="PLX22_05735"
     CDS             12789..>13060
                     /locus_tag="PLX22_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19432.1"
                     /translation="MKKALMAFCDSDGAIYKHPAAIAAFRSGRRFVAVEQEELMPLPD
                     GSTLYVLPGRFPVEGRSFKSVSNFNGNEILAVAAFLSSGYLRTYLPA"
BASE COUNT         4188 a         2169 c         2829 g         3874 t
ORIGIN      
        1 ttacaggtgt ataatttaat tatcaggatt ttattaatga aattaatgaa tagaaagatg
       61 gttattacag cagtggtttc atgtatggtg attgcagtaa gtacttttac catttacaac
      121 aataatagaa aaaccgttaa accaagggta acctcagttg ttgatgcggt atatgcgtta
      181 gggattgtta ccccctataa agaatatagc ctgcgtgttg gcatgagtac caggattgaa
      241 aagctctata tatgggaagg tgatagggta caggcaggaa gccctttagt gcaacttgat
      301 ggaggcatac tattgcgtgc tcctttttcc ggggttgtgg ctaccttaaa ccgcaaggaa
      361 agtgagatag ttagtgctca ggaatccatc atgacaatag ttgatcctgc aacaatgtat
      421 atcaggcttt cacttgacca ggaatcgatc attaatgtta gaaaaaaaca ggttgctgag
      481 attagctttg aaaacctgcg caataaaaat atacgtggcg tggtaagcag gatatatcca
      541 tcagaagggg agtttgttgt tgctattgat gttgattcat ttcctgaggg cgtattgcct
      601 tacatgacgt gcgatgtggc aattaaggtt agtgaaaaaa acaatgcgct attactgcca
      661 aagaatgctg tccacaatgg tgctgtaaca gtaattcgta acggcagaaa acaattggtg
      721 aacgtcagca cacgtgcaca cgacagtgga tgggtggaga ttgttcaggg caatataacc
      781 cctgatgata ctgtggttgt tatgcgatga gctattgcct aaaaaaggaa tgcggaaatg
      841 tttgagttag caatgaaata tattatctcg cgtaaggttc aatcgttgct tatgctaatt
      901 ggtgttacac tgggatgtgg cggctatatt atctttacct cggttcaact tggtttccag
      961 acatttatga aggagcgcct tatagaaaat aacgggcata ttaccattga aagcagggaa
     1021 gaagatatta cccgtgagtc aatggaaggt gtttttttta aagggagcac tgtttactgg
     1081 ataagcggtc caagcggcag gcgctccaat gagtatttgc aaagcgcatg gcactggcaa
     1141 aaacgcctgg cagatattcc atttgttgta gcaagtacaa gacgtgttga ggcacaggtt
     1201 acctgcgcac ggggagcttt ttaccgttcg gttacactgc gtggtattga tcccttttct
     1261 gaaccaaagg taaccaatat tgctgaagat attgttgtgg gcaacatgaa tatgctcaac
     1321 agtggaaaca accttgtcat tgttggagtt gaactcttga agtttttggg aggtaagctc
     1381 aatgatacca ttactgttgt gaattctaat ggcaatgtgt acccggttaa ggttgttggt
     1441 atctattcat caggtgacag aagaaatgac agaacgttga tactggcttc cattacgact
     1501 gtacaaaata ttctggaaag ccctggcagg ataaccaaga ttgtagtaaa gacggtaaat
     1561 gcggatatgg catcaacatt tgctcatgaa ttatcgcagg ggacgtatga taaggttgaa
     1621 agctgggacg aggccaatat caattttttg agcatgacac gacagcaatc gtttatacgg
     1681 aatattacca tatttacctt tatactggtg gtttcatttg gtatatacaa tgttctcaat
     1741 atgcttgtac atcaaaaaca gcgtgagata gcaatactgc gttctattgg gtatacccgt
     1801 aatgatacga tagtgctatt tttattgcag ggagcaattt tgagtgttgc tggcgggatt
     1861 atagggattt tgattggcta tggtgcatgc agctatattg aaacaattaa aatgccaggc
     1921 agaccaatgc atgttgcatg ggatatctct atttatatgt ggggatttat gcttgttgtt
     1981 atatcagcgc tttttgcaag ctttttacct gcaaaaactg ctggttcact ttcaccaatt
     2041 gagattatac ggagaacgga ataatggtgg cgatagaagc caggaatgta gtcaaggcat
     2101 ttggtgattt tactgcctta aattcaatct cgctcactat cgccagaggt gagtttgttg
     2161 ctataaccgg taaatccggt tcgggaaagt ccaccctgtt ttattcgctc agcgggcttg
     2221 atagcattaa taatggtacg gtgatgatgc tgggatatgc cattcactcg ctaccggaag
     2281 atgaggtgca caggattaga aacacaaaga tgggttttgt atttcagttc cactatctta
     2341 tttcagaatt aaccgctctt gaaaatattt tgatgcctgc aaaaaaaatt aaaatgcata
     2401 aagaaaagat gcagtatgcc cagtacctgc tggattactt tgatattaaa cactgcaagg
     2461 ataagcttcc ttcgcagatg tcaggtggtg agcgacaacg ggttgctgtt gcccgtgcat
     2521 tgattatgaa tcctgatatt atctttgcag atgagccaac aggaaacctg gattctataa
     2581 acagtgaagc agtcatgcgt atttttaaag aggtgaacaa acaaaatgga acaacggtga
     2641 tactgattac tcatgatgat gagtatgcac atatggctgg ccgacagata catttaaaag
     2701 atggacagat agagtatgat atttacaata aataatatca tgatatcatt gcatttttaa
     2761 agagttacca atagttgcgt tgttccatga aaatagtggc aatggcatgc tgaaacaaaa
     2821 cctatagtca ttctgcggct tgattcagga tctatgagaa ttatttagat tccgtgtcag
     2881 gccatgaatg acattgatgg gataatgcta ttttttaaag gagtattgct aaggatgaaa
     2941 aataaaaata taacagcata tatcgtattg tttatggttg taagtgtgca tggttttgct
     3001 caaaacaatg acgcaatcct ttcgtatgat gactatgtat caatcatgat taaacagttg
     3061 ccccagatta aaaataacat cattcaggtt aaaaaagcac aggtaaatgt tgaaaaagct
     3121 aaaagtgttg atgatacaac actggttgct caaggacaat atacccatca aaaacagtat
     3181 acaaccggta aaaacctttt tgaaccggat tatagtgaaa catactatgg ttacgccgga
     3241 attgaacgta tcattgcacc aacaggaaca cgattatcag cagggtttga atatgatgct
     3301 tcgtcagtaa aaggtagccg tactatcccg gcacaggcta tgtcaattga tgaatatcag
     3361 ccatccatca aggcaactat cacacaaccg ttgttaaaaa atgcatttgg tattgctgat
     3421 agatatggaa aaaacaatgc tgaatatacc ctgcaaatac aaaaattgca gcagcttgag
     3481 gatgataact cggtcatgaa ctactataag aagctgtatt ttacatggat tttttataaa
     3541 caggcaatcg atattttagc ggaaagcatg gcaaacgcta agaatcttgt tgtgactgtt
     3601 gagcgtaagg ctaaagcagg gctggctgaa aatgatgatc ttcagcgggc atactcttca
     3661 ttatacagct atcaggcaga ctatcagcaa tatcagatta cctatagagc tcttttagat
     3721 gagctatggc tttatatcaa taaggaggca acacctgata gtgcatatct tcaaaaatat
     3781 tttgatgaga tagttactat ggatatacag gaaatccctt ttgaaaaaac acgtgcctat
     3841 gccattgttg ctaaaagtat tgatagcatg cactatacca tagatgttgc caataatgac
     3901 acgctgccac agatggattt agttctttca gcttcacgaa aaaattatag ccagaaccag
     3961 agtgatgcgt taagcaaact tcctgatacg gattactcaa tagggttaca gtttagctac
     4021 ccgttgggca atcatacgtc ccaatcacag ttacaggaat atgagctatc ccttaaagaa
     4081 ttagaacaca acctgaatat atcaaagaat gattactata aaaatatccg taccatctta
     4141 caatcgctga aaggatataa agatttaata gaaataaaca caaaaaattt atatgcattg
     4201 gtatcccagc ggcagactga aagaaaaaag tatgagcaag caaggctgga tcttaaatat
     4261 ttaattgaaa ctgaaaattc aattttttct cagcagctaa aattattaca gttgaaggtt
     4321 agttttattg attattttat tgattataat gatttaatcc agtaatagta tatgtctgat
     4381 tgtatttaaa tagttaaaca tgtcattgtt accgatatta tatgacattc taatgtttca
     4441 ggagattgtt atgtatcaga ttatttcttc atcgtttcaa agcaatgatt gtttattaga
     4501 tgcccgcctt tacctgccag agagcaatca aaaactgccc atcatcataa tggcgcatgg
     4561 atttggagcg aggatggatt ttggactgca tccgtttgcg gatgtctttg caaatatggg
     4621 ttttgctgtg cttatgtttg actacagggg ctttggcaaa agcaagggga gtcaacaaca
     4681 attggtgtat cacaagtatc atattgagga ttatcatagt gccatacact atgtaaaaac
     4741 cacgccaaat attgatggtg caaagatagt attgtgggga acatcgtata gcggcgggca
     4801 tgttttaacg gtagcatcac aggatactac tattgctgga gttatagcgc atgttccatt
     4861 tgtggatggt attgccacag catttcatct gcccataaaa aatataatca ttggtatgta
     4921 ttcaggggtt cgggatatgg tgacacacat tgttaacagc aagccctata ctatacccat
     4981 tgtatcgccg ccagattctt ttgcggctat gaatactgct gatagctatc agggttatat
     5041 gaatttagtg ccagcagagg taactgatga gaattggtgc cctgcacgtg tatgtttaac
     5101 cctgccattg tacaggccaa caacatatgt aaaaaaaatt atgtgcccgg tttgtatcat
     5161 tgctgcagaa tatgattcgt tgattccttt atctgctgtt aaaaaagctg ctggtaacat
     5221 aaaaaacatt gattttcata tattgccgtg tggtcatttc gagccgtata caggaacaat
     5281 gtttgaaaag agtataacca tacagaagag gtttttagaa cgtttattat aatgctctgc
     5341 cccctttttt aatagttact ttcgccaggt aaaatacaat gacgtaccac gcgccaaata
     5401 tgatatccca atctgtcaat aaaacgaatc gcttcgataa actcaagagc agtatgtgaa
     5461 tcaagtgatc ccatggcagt tttctttaat acttctattc tttctaattt acgaatgtta
     5521 gctaaatatt gcgatagttc ctgtattttt tcaacattat ccaatgaact cttttgttgc
     5581 aatgcatttt ctgattcatc aagttcattg gaaaagtttg atagcatatc tttaaagaga
     5641 tcactatttt tgagtgtatc aattgcttgt ttttcgtaac atgcttcgat aatactgttt
     5701 aaatggtcaa ttgaatggag tatggagata tgttcattgt aaagtttact ctcatgtgga
     5761 ttggttttaa ccttgctcat aaagagcttg gtttcattga gggcatttgc agcaaaacgg
     5821 agtcgacgct ggaaatcatc ttctttatct tgtagtatct cttttaatgt atgaataata
     5881 aaaagggcaa tgtcagtgac agctcgtcgt gctgcttcta ctgctatggg tggattttta
     5941 gcaatagcgt catctaagtg ttgtgttaaa tataattgtt tatcaatgaa aaaagttcta
     6001 aggaaacgta taaatggtgt aataaagggt gtaaacagca atatgccaat acaattgaat
     6061 aatgtatgga acaatgcaag ctgtatttca taaggcattg ggtagatagt agtaaataga
     6121 aatgcaagca atggcgataa ccatggtatt ataataaata atatgattgc tgttatgata
     6181 ttaaaaagaa aatcagcagc gataattcgt tttgtatcag gtgttgcacc aatgcctgca
     6241 aacagtattg ggatagattt ccctgcattc gttccaatga caagaacctg tgcctgaaaa
     6301 agatgaatgg tgcccgtatt taaagctatt agtgctattg caacagaagc acttgaggat
     6361 tgcattaaaa tggttagtac aaagccaata gcaaaaagta ttaatcggtc aaaaatgcct
     6421 tctccagcaa tgtttgaaaa gtttatgatt gatgacagtt gtgacatgcc ctgttgcata
     6481 aggctgatac caaaaaaaaa caatccaaaa cctgccatga tcatgccgat atcgctcttt
     6541 ttgtcagtat aaagaatttt taatatgctg cctatagcaa ctaagataag agatatattc
     6601 cctatactat attgcacgcc aaagattgcc acaagccagc ctgttactgt tgagccaaca
     6661 tttgcaccaa agattactgc aagtgcctgt gacatggtaa tgagtccagc acttacaaaa
     6721 ccaatagtgg caatggctgt agcactggag gcttgaataa tggttgtaat gagcataccg
     6781 gtaaggagtg ctttgcctgg acctccggta aggttggtaa ggataatacg cagccgtgaa
     6841 cctgccagtt tctgtatgga ttcggacatg agagctatac ctaaaaggag cagtcctata
     6901 ccgccaatgg atgttattat atcagtcgca ttcatacggt aactcttttt actatagtat
     6961 cggcaattat aggatagtta tatgaaagat agtatttaga gcaagattta ttttttataa
     7021 tgttatattt attgttcaag aatatgaatg cactcataat taagatgtaa acaaggcttg
     7081 aaagagatga aaatgcttaa aaaatgcagt tgagattatt gacaacacaa gatataactt
     7141 tgtgaaaaat gggcaacata agaaattact ctcgatacta taatcaggtg tgtttgaagt
     7201 tacgcaatta taaataaaaa tttgaaaaaa ttccttgact tccgcttcat ttttaatctt
     7261 atattaaaat caaataagaa attctatgtg ataaatgcag tttggaaatc aagagataaa
     7321 attcagtgaa cctgattatt attgggtcga tatgttggtg attattaacg aagattattg
     7381 gtaaaaatga gggaagccag atcaacgatg tctttctaaa gattgtatat actgttataa
     7441 gcaatcatat cgtgaacata tttttactca atagaacaat gttttttgaa taagcttttt
     7501 atatttttga ttcaaaatat agattaatta aaatggagga aacgaaaaat gcgtgcaact
     7561 catcttattg ttacgataac atttgtactt atgagctatg tttctgttca ggctaaaaca
     7621 gttctggatt attattgctt aataccagac gagttttttc aatgtgagga tgatgtcaag
     7681 ataacccgca attataagct caatcagata aaatacaaga atatcaaagc gggttacatt
     7741 cgcgctgagt cggaaagaaa tcccatggaa gtggcattgt ttaaggacat aaaaagcaac
     7801 aaggatgttg tggctgtgaa cattaagtgc ggaccaggtt gcatgtgcaa ccggtttgct
     7861 cttttacagt ttgatgcaaa tggcaaggct atcaatatca ctgcttctat ttttcccaat
     7921 gaaaatttga tatggaagaa aattaaaagg accgatgtgg atgcatacga atttattctt
     7981 cccgaagttg gtaccaccat taaagttgta cacgctgata cgggtgaaga attggcacaa
     8041 attttctggc gtgatggtaa atttgtgtta cagtagaaaa acaaataaaa aagttatggt
     8101 taagtattaa gtacaacacc ttgtgttaag ggtgttgcac tgggcgtata acaatcaggg
     8161 gagataatct tctctgaggg gcgaaaacac ctcaattgtt gccaatcagc tcaatgaaat
     8221 gtactcaatc aatagtcagt aactatcgct cttaattata atggaacaac tggagaaaac
     8281 gatgaaagta aagaaattac gatgtgaaca ctgctcgtta cgagcaactt ttgacagaag
     8341 tcctcgttca ctgattggca gactatggcg ctggcatatt acatggtgtc ccggatggaa
     8401 agcgtatagt aattctttaa ctgaagaaga acgaggagag ttgatagcaa aatattcatt
     8461 ataaatataa aatagggaaa catattgcct atagttttta tacgttgcac atccatggat
     8521 ttttgaagaa gcagtctttt ttaattattg tgatgtttat ggtgaaataa caattgattg
     8581 agctttcaca gtgtcttcgt caaaatctga caggataagt attactatac cattattcac
     8641 atttaagata aaaaatactg gtaaaatcct cactatttaa caattattca ataaaatcta
     8701 ttgacaaaat ttggtttgga ttaattaaat tgtcacttac aaatcaataa aatgaaataa
     8761 gtgtaaagta tgcaatttaa agttttgcca tcttcattaa aaggtgaagt cgttataccc
     8821 ggttctaaat cgcataccat acgggcctgt gtatgtgctc ttcttgctaa tggtacttca
     8881 accataaaaa tgccccttga ttcaagtgat acacgttcct gccttggtat gattcaacgg
     8941 tttggggcaa cggtaacaca gcacaacgat gaatgggttg ttaccggttg cggcattcct
     9001 gccgttccgg atgatatcat tgatattggc aattcgggga catcgcttta ttttggaatt
     9061 ggtgttgcat cgcttgtcaa tggtatcacc gtattcaccg gcgacaatca gatacgcaac
     9121 aggcctgcgc aggacctgct tactgcaatt acagaattag gaggctgggc agtttcaacg
     9181 cgtagtaatg gcaagccgcc ggttattgtg cagggaccta taacaggagg gcatgttgca
     9241 atcagggcaa ttacctcaca gtatgtatca tcactgctta tagcaacacc gatggcaaaa
     9301 aacaatacta tcattgatgt gcccctgctc aatgaagctc cctatgttgg catgacattg
     9361 tggtggctta atgagcttgg tattgaatat catgctaatg ggtatgacca gtttattata
     9421 cgtggaggtc aaacctacaa accattttca aaatacatcc ctgctgattt ttcatcggca
     9481 acgtttttta tggttgcggc tgccataaca ggtagcacgg taaaacttaa ggggttagat
     9541 attaatgata cccagggcga taaagaggtg gttacaattc taaagcagat gggcgctgat
     9601 attgcaatac ttcccgatgc aattgttatt accggtaagc cacttaaagg tggcgagttt
     9661 gaccttaatg caattccgga tgctcttcct gcactgtcag tatgcggatg ttttgctgag
     9721 ggcgaaacgc ggctggtgaa tgtaccacag gcacgggtta aggagacaga ccgaattgca
     9781 gtgatgtgtg ctgaattaaa aaaaatgggt gctgatatag aagagcttcc tgatgggctt
     9841 gtcatcaggc aaagtcaatt aaaaggtgct cccgtacagg gacatcatga tcatcgtgtt
     9901 gtgatgtcgc ttgcagttgc cggattggct gcacaaggtc ataccgttat tgatacagcg
     9961 caggcggtgg atgttacctt ccctaatttt gttgaactta tgctgcaact gggtgctgca
    10021 attgaaaggg aggagtcatg atgctcaggg gagtgcgtgg agcaataacc gttgatgaaa
    10081 atacaaaaga agaaatagta tcaaggacca aggagctttt gcaggcgata gttactgtca
    10141 atgatatcca tactgatgat atagcatcag ccatatttac tgtcactgat gatttgaatg
    10201 ccgagtttcc tgcagttgct gcccgtacta ttggctggat tcatataccc cttttatgtt
    10261 cccgagagat accaatccct ggttcactgg gcatgtgcgt acgggttctt ttgcatatca
    10321 ataccaataa aaaacaggat gagattgtgc atgtatatct gcgcgatgct aaaaagttgc
    10381 gaccggatat tggtacacct gagcaggata aatactatct atcgcacgat tgagaagaat
    10441 tgtgcagatg aatactaaat aaaaataata tgtaccgggt acattcaatg aagcaaatca
    10501 agtatttagt agtgtcgatt atatgctatg gagttgttgt aagcactaat gctattctgt
    10561 atgcacaggc tgaacgtgaa attgaactga ctgctcagga gatattggca cgagttgacc
    10621 gaataatgca atatcctgat ggcattttaa aaggatatat catgtatatt acacctgatg
    10681 gttcatcgca aaaattacag attacagggt atattgcaaa taatcagttt ttatttgatg
    10741 tcagcaaacc tgcacgcggt agcatgcaaa aaatattgta ccggattggc ggtgaggatt
    10801 tgtgggtata tgatatggca gcgaaagcac tataccataa aatgggtgtt gataagtttg
    10861 atgatatact ggcaaccaac tttttttata ctgatctgtc caatgctgat ttgcagagca
    10921 attatgttgc aacgattgat ggctctgtga ttataaagaa taatgattgt tacaaactaa
    10981 cccttgatcc aattgataaa accggtatct atggaaaatt aaccttatat gtggtaaaaa
    11041 aaacctatat cccattgcgt atagattacc atgataggga taaggttatt tttaaaacac
    11101 tgtctataac aaaagtagcg gagaaggatg aaaaatcatt ccctgtacga tatgatatgc
    11161 ttgatatcaa aaaaggtaca atgacgatac ttgaatttaa tagcatagaa gacggtgtca
    11221 gggttgatgc agagttattc agaccggaga agttaggaga ttgatggtgg cacagttttt
    11281 tgtcgattct gctggcagtg ataatagcat catcatcacg ggaaaggatt tttatcatct
    11341 aacaacggtg cgccgcgcaa gcataggtga tgctatacag ataattgata ataacggtat
    11401 gcaataccat gcaaccatca cacaaataga tgatggaaag atagttgcaa caataacaga
    11461 gaaacttcac tatcctgata ttcatgtaca gtgtacattg tgtgctgcac tgttaaaggg
    11521 caaaaagttt gaccttgtta tccagaaagc tgttgaaata ggagtaaaac aaataattcc
    11581 tgttgttacg cagcacacca taccggatgt ttcgggcaaa gaagataaaa aggttgaacg
    11641 ctggcagtca attgcaagcg aagcctcaaa gcaatgctta cgcaaagagg tgccagcggt
    11701 tcataatgtt atgccttttg aagatgtcat aaggcttcca aaagagattg gtattatggc
    11761 acatgctgac cccaaagcga taaacttacg gaatttttta aaagaacagg taaaacataa
    11821 caacgttatg ctgctcatag gacctgaagg ggggttttca aaaaaagagg ttgaaattgc
    11881 acgacaatat gattggtttg tactatacag tggtgcaaca cagctcagag ctgaaactgc
    11941 agctatcgtt ttgccggcaa tcattttata tgaatgggga tatgaataac aggatagtag
    12001 tatgaagatc agggtaaacg ggatagatat tgaatttgag aagaaaaccc ttaaagagct
    12061 tcttgatctt tacaggttta atccatcaaa aatcgtggtt gagaaaaatg ggcgaatcat
    12121 tgataaagaa cagtatgcaa ctgaagtttt gaaagatggc gacgttcttg aaattgcgcg
    12181 atttgttggt agaggatgaa tgacaaattc catgctctga cggaatcttt gcttacacaa
    12241 gaagaactgc aacattgcat tttgttgttg ttttgcctgg gcgatagctc atgtgatgac
    12301 ccattgcttg aagaattgca aattcggggc tatatcgtca aagaaaaaga taatcgtatc
    12361 attactcaaa agggcaaaca atatctatca gggcttatgc aaaagctggc acaatttcat
    12421 aatgcaggtg aaattatttt ccagcactat gttgacaggg aactatcgcc aataaaaaaa
    12481 gagatacatg aaatacgcat gcagtggcgc gctgttcgtg ataaacgatt agcgcgtaaa
    12541 gcggaattgc aaaaaatggg gaaggataaa tcagctttgc aaaaggatag tatttataaa
    12601 gcactgcaaa aagagcagaa tgcattgcgc acacacctga aacataccga aaaaaagtat
    12661 cataaaaaat tagcaaatca tgtaaaaaat gtaggaatgc tcccctgaaa tacatcatgc
    12721 aagggtgcat actcaatggt gatgcagtga tactattaat attattgaat gcggtgtaaa
    12781 acgattgtat gaaaaaagcg ttgatggcat tttgtgatag tgatggtgct atctataagc
    12841 atcccgctgc cattgcagca tttagaagcg gcaggcggtt tgttgccgtt gaacaggaag
    12901 aactcatgcc gcttcccgat gggagtacat tgtatgtatt gccgggacgg ttccccgttg
    12961 aaggaaggtc gtttaagtct gtgagcaatt tcaatggcaa tgaaattttg gctgtggctg
    13021 cttttttaag ctcgggctat ttaaggacat atctaccagc
//
LOCUS       DAOSOX010000055        12848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4865_length_12848_cov_8.545689, whole genome shotgun sequence.
ACCESSION   DAOSOX010000055 DAOSOX010000000
VERSION     DAOSOX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4865_length_12848_cov_8.545689"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLX22_05740"
     CDS             complement(<1..323)
                     /locus_tag="PLX22_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HOT19433.1"
                     /translation="MNYWQEYNAKLTTPDNAVKVVDCGDVVDYGFFNGKPVILDQALA
                     KRAPELKDVVIYTAVTLPPVPEVIKYPQSFTYIDWQWSKLTRLLHTQVEAAYYCPILY
                     HRAPF"
     gene            753..6305
                     /locus_tag="PLX22_05745"
     CDS             753..6305
                     /locus_tag="PLX22_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOT19434.1"
                     /translation="MKKTSKMLISTVIGIFMVSIVFAAALVIESNEIKLVIDYQVEKP
                     YTEMALSFSKDIFVLSDVDSMVKDVNDKLHITFKPDIEYTIKPLDTRSCIVEIYNPKP
                     ANEYHFTITVKGYDKKAITITCNNSEIEQGKDYYFRTPLLKVVEYHRSSEMINTPIVL
                     RFNFPVSLEVLKQKLSIDPKISVAVEYDKDDTTKKTVIVYPANEEKAQKYSLTINKSM
                     VPVGGVLGMESHFSFTYNTFYPLELMKVESAYGRSGSFYPDYPVEFTFNNEIAIPEGH
                     SAAEYVTVSPNPGGVQVSVYGDTLSISGNFIGNERYTVTIAKDIQDIYGQKLDEPIVE
                     EITFEHAFSHFTCPENYMLVESYMEKIVPMHMVNVSSFSCKYLSLVDERDIRKYLENP
                     KEYFKEYATVKHYTVNWAWDVYKTVKFDFSKLHAANHGFFVYEITPSMENPYNSESNT
                     FHGVMQLSDIGVTVKKTKNNVFVHVRSLLHNTPLQNASVYCTGDSPIGKTDKKGVAII
                     KNTGCSVYTIKHEQSIFYYSMSNSSYHDEDNSNTAIQLPEKHYGSSYFINTPKIQLFT
                     DRYLYKPGEIVHVKGIFRYRNNDQWSLNPPLSPVKDIIVTVYNSRHEEIETYTKSLSP
                     TGGVQWDIPIKGNYPTGYYRITITAKNKDYNINGGIHFQVEEFKPAKAEMKIIPEKLK
                     YHWGEMLAMDVFGWYLFGAPVNNDVEYTVSLTPVIYRSESYPDYSFSTQLYYYDEYDE
                     YTPSSTTIATGRVKVNEKGVARVFVPLKAMVNVPNADIVITAKTYLSDDSPVFGAKAG
                     LQVFEPVHVGIRVPRYFNNTDSPVTVQLVALNSSDKVVEGNTVKLVVKKHEWKSFQIA
                     GVNGRLQWEYKNIATDVYAHNYVIGKKEVAVAINDPGYYTAEVYDSSGKRKFAVKSFY
                     SIGKGEAGWRVNDDESVDVETDKSSYNVGDTAMLLVKNPFEKASAMVTVEREKFFDYF
                     EVPATTSIVMIPIKITEEHIPNIYVSVMVYTPRTGFNKVKHGEDLAKPKCYIGYASIK
                     VVPREKKLQVKVKSDKKQYKPGEETTIEINVTNYKGQPINGEATISVADRGVLNLVNY
                     TLPNPLDFFYAPRSLAVTTFDVRQIILGQRYIKEKGELIGGDGGIASMGMIVPRANIR
                     FSAYYNATVKIEKGVARIKFKLPDNLTSFKSMVVVHTNDSRFGYGETTFEVKTPLMVL
                     PTFPRFARVGDAFDAGALVFNYSGIKQTITVSMELSGGIKCQDGKLSSIKEIDLNNGQ
                     STELTFPIVVDNANQSTFVIKAKADKHTDGIKDSIAIKAPNIYETVAQYGRITKADDS
                     AKHTIDISKNIIPYVSSVNVFVSPSAFSELKGSLDYLVEYPYGCLEQKASRILPLIIG
                     EDIIIQKNLLKIKSKEDLKNTVATILKEFPGYFKNDGFSYWQDSSYTSSYLTVYATFI
                     MTMAKKQGYTVDAVTFDKALATVKRFATKGVIFSNEESFFSTYYKYLTLSFAHYVAAL
                     NGVTSENQLKLVYSVMNSKYEDMIAGYAYLLKAVALYPDSNFKQSMTRMIAQKFFSAM
                     RTESGYVYFKDASDWGYFYYDDVIVTSLILQSLLEAGVPFDNDYKVVNYLLKQRKGYA
                     WLNTHENAIVWWALNTYFTKYEKGKPDFAVTVLFNAKELLNALITDTSTTYSGLYAIT
                     HQDSGSRSLQLSKKGEGVLYYTFRYTYIPVYDKMISKNMGFLITKRFKDVKTGNEVTA
                     FKKGSEYLVEIEVYTPKERGITVLDDQLPAGFEPVNITFATEEKMTAEKRNAYYADWG
                     TFNHYEQYNDRVVYYADFMHTGNHTVVYKVRATNSGVFKLPACKVEEMYNPEVFGIMY
                     YTNAVQVQ"
     gene            6302..8590
                     /locus_tag="PLX22_05750"
     CDS             6302..8590
                     /locus_tag="PLX22_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT19435.1"
                     /translation="MIAMKPLLTLECRYRIKKIIKLSSGVIFLAIVIYIVFPYKRDYL
                     ASSNKLPVVIYDRNGTVLMEVAKGKSGLCQAISIQQLPEDFISLLLFSEDRNFYHHWG
                     ISLKSIARALYQNGKAMRIVSGGSTITQQVVKSKKEILRNTVLTKMLEFIEAVRMELH
                     FSKDEILQGYINEVYLGNNIYGFEKAAQIYFGKHINECNLLEAGFLIAIVKAPERYNP
                     YQNPETVVQSAKRLLRNACHMGYVKISPFELEVYTNSRIALQYNEKKVIAPLFCLYAL
                     SQARRLFPDKDITHIYTTLDATIYSNVLTVMQNSMSMLKDNNALHAALVMIDNTTMEI
                     VAMIGSVDFFDYKKGQVDATLIKRQAASTMKPFAYALALETGMFHTSSIVPDVYTQFP
                     SKVGNYIPKNFSDSYHGPVRFAKALGCSYNIPAVYVVSKIGLVSYYNLLKKIGFDSIT
                     RSPSYYGLGLVLGNADVSLLELANAYTVFPRQGLFSKTIAIKKIRDAHGKEYTVQSAG
                     SARVLKQQTCYLISHILSEYKYKVDAFGLCSSINFPFPFAVKTGTSKDFKDNFIAGYN
                     KQYTFAVWVGNLYNESLCDLPAVSGAGIVLKDVLLYLWNAGYPFERFTMPENIRKVKI
                     CTLSGMVADTYCHSTDYELYSGNNQPCNKCNWHTKNATIVPSQFKEWARNKNYNVAHD
                     ASVQIVFPGNGVVFKIEKTVRKNLQAIPLKATANSNIQWYVDNRYIGEGSELIWQLSA
                     GHHVISIQAGNQRDAVNITVLE"
     gene            8772..10865
                     /locus_tag="PLX22_05755"
     CDS             8772..10865
                     /locus_tag="PLX22_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT19436.1"
                     /translation="MMHKLFYPDSIAIVGLSSRPNNIPRLTLENTLRWGYSGRIFGVN
                     PKSDDEQVDGIRMYKSIGALPEIPDLVYALVPAKFVPGIVEECGQKGVQWMAIPSGGF
                     SEYSEEGKSLAQKTVDTAKKYGIRFVGPNGLTVANVENGLCLPFVPILRPQFGGMSIV
                     SQSGGVALMMWNVIMDENIGIAKFASIGNKLDLDEVDFLEYLGSDPATSIICMYLESI
                     PRGSDFIKVADRINKPIVVYKSNTTQAGKKAAMSHTAAMSNDEDIINAAFEKAGIIRI
                     YNYHDFFAVAKAFKLPPMKGKRVMVMSPAGGFSVMTADLCEQAGFKFANPGKEFYESL
                     QKFSNAGVIQFSNPLDMGDIYDPAFTAHVLYSVMHNNNVDGAMYVSQRPQMPDRKNVF
                     SQMFLSDLSKEAWGAIVSSGKPLAICLLGMAKLMAQIKRSVNFPIFNSAEEMVRAMKF
                     QMKYWLRRSTPQMHESYPMSLDTVLRWITNHHGDIGEDAMELLEFIDIPVPQSGIAKS
                     EKEAVTVAKQLGYPVVCKVISRDILHKSDVGGVIVNVNGDEGVKQAYAAIQSSVLSHK
                     PDAHIEGIRISTMAPDGVDMFVGSKYDQSFGQVIIFGFGGIYVEVFKDIAYCLCPATK
                     EEIKQKLTSLKSYALLKGARGKSALDVDAYVDIIWKVSHLLASASHIKELDLNPVRVF
                     EHGCQVLDVRMRIVH"
     gene            10903..12048
                     /locus_tag="PLX22_05760"
     CDS             10903..12048
                     /locus_tag="PLX22_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT19437.1"
                     /translation="MAMVYLDNAATSFPKPDTVIKAVTNCLTNYCANPGRSGHSLSIE
                     AARKVFETRETVAELFHAKDSRYVIFTANATMAINLVFHALLQKGDHVVVSSMEHNAV
                     MRPLRHWESQGIITLSLANADGNGFVDDDIGAFIQPATRLCVINHASNVNGVVQDIAS
                     IAGRIKEINPDVAVLIDASQSTGLIPIDIQAIKCDFVAFTGHKALYGPQGIGGLVINT
                     AKHLQPFIMGGTGSKSESQFQPQFLPDKFESGTLNLPGIAGLKAGIDYVKSRMDEIHH
                     NELLHIKTIVEGLSSIKDLKLFWSRSLHNQIGVISVAHNHIPCSEIGQRLNDEFSVFV
                     RTGLHCAPLAHETLGTYPNGTLRVSVGLFTNKSDVDVFCKAIEIISK"
     gene            12054..12305
                     /locus_tag="PLX22_05765"
     CDS             12054..12305
                     /locus_tag="PLX22_05765"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOT19438.1"
                     /translation="MDYCYLLLPSVHDAMKAEKLLLQSGCAIQVIATPREISHNCGVV
                     IKFSCAEINTVLLSCKELSAEQKIFKKNSNNVYYEIIPE"
     gene            complement(12292..12438)
                     /locus_tag="PLX22_05770"
     CDS             complement(12292..12438)
                     /locus_tag="PLX22_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19439.1"
                     /translation="MIQNSFDMKQWKMEQIKYFKKYQSTFPKNSKEYKILAESIRKLE
                     STLG"
BASE COUNT         4054 a         2205 c         2754 g         3835 t
ORIGIN      
        1 tagaatggtg ctctgtggta aagaatagga cagtaatagg cagcctctac ctgagtatgc
       61 aatagtcgtg ttagtttgct ccactgccag tcaatatagg taaaactttg aggatattta
      121 ataacctcgg gaaccggcgg taaggttacc gctgtatata taaccacatc ttttagctca
      181 ggtgctcttt ttgccagtgc ctgatctaaa attacaggtt tcccgttgaa aaaaccataa
      241 tcgacaacat caccgcaatc aacaactttt actgcgttat caggagtagt taattttgca
      301 ttatattcct gccagtaatt cataaatacg cctccatttt ttaaaaattt taattcaaaa
      361 tattataata atacaatatt atattgtcaa taaaaatatt tagtattatt aaaaattata
      421 ttaataataa attatatata tacgatatta ttataatgtt gtaatatata tatctggcat
      481 tcaattattg aattaatccc caataatata ttatttgctt tacacagcag atgtaactat
      541 ggtaacatta tctacttaat aatacaaatg atgatgtttt aatagaagaa gttgcctgtg
      601 gacgatgtat agcaagccat agaggatgaa aatagtgctt ttactttttt taatacggtg
      661 gcatgctgct ttacttccat ggggaaggaa aaaattatat gatagccgat accataccat
      721 agtattccac aaatgaaaag gagaaaattg ttatgaaaaa aacatctaaa atgcttattt
      781 caacagtcat tggtatattc atggtcagca ttgtttttgc tgcagccctt gttattgaga
      841 gtaatgagat aaaacttgtt attgattatc aggttgagaa accatatacc gagatggcat
      901 tgagcttttc aaaggatatt tttgtgctca gcgatgtcga ttctatggtg aaagatgtca
      961 acgacaagct ccatataacg tttaagcctg atatagaata taccataaaa ccacttgata
     1021 cacgcagttg tatcgttgaa atctacaatc caaaacctgc caatgaatat cattttacta
     1081 ttacggtaaa aggctatgat aaaaaagcta taaccataac ctgcaacaat agtgaaatag
     1141 aacaggggaa ggactactat ttcagaacac cattgttgaa ggttgtagag taccatcgaa
     1201 gcagtgaaat gataaatacc cccattgtgc tacgatttaa ttttcctgtt tcattagagg
     1261 tgctgaaaca aaaattgtct attgatccca aaataagtgt agcagtagag tatgataagg
     1321 atgatacaac aaaaaaaaca gttattgtgt atcctgcaaa tgaagagaaa gcccaaaaat
     1381 attctctcac aataaataaa agtatggtac cggttggcgg tgtgcttggc atggaatcgc
     1441 atttttcatt tacctataac accttttatc cattggaact gatgaaggtt gagtctgcct
     1501 atggacggag tggatctttt tatccagatt atcctgtaga gtttactttt aacaatgaga
     1561 ttgctatccc ggaaggtcat tcagctgcgg agtatgtgac agtaagtccc aatcccggcg
     1621 gcgtgcaggt cagcgtgtat ggtgatacat tatcaatcag tggtaatttt ataggcaatg
     1681 aacgatatac agtaactatc gccaaagata ttcaggatat ttatggtcaa aagctggatg
     1741 aacctattgt cgaagagatt acctttgagc atgcattttc gcattttacg tgtccggaaa
     1801 actatatgct ggtggaaagc tatatggaaa aaattgttcc gatgcatatg gtcaatgttt
     1861 catcgtttag ctgtaaatat ctttcacttg ttgatgaaag ggacatcaga aagtatcttg
     1921 aaaatcctaa agaatatttt aaagaatacg ctaccgttaa acattatact gtcaactggg
     1981 catgggatgt ttataaaact gttaagtttg atttttcaaa gcttcatgcc gctaaccatg
     2041 gattttttgt gtatgagatt acaccgtcta tggaaaatcc ctataacagt gagtctaaca
     2101 catttcatgg cgttatgcag ctttcagata ttggggttac ggtaaaaaaa actaaaaata
     2161 atgtttttgt ccatgtgcgc agtttgcttc ataatacacc gttacaaaat gcctctgtgt
     2221 attgcacagg tgatagcccc attggtaaaa ctgacaaaaa aggtgtggca atcattaaaa
     2281 atacggggtg ctctgtttac accattaagc atgagcaaag cattttttac tatagcatga
     2341 gtaatagttc atatcatgac gaagacaata gcaatactgc gatacagctt cctgaaaaac
     2401 attatggctc aagttatttt atcaatacgc cgaagattca gctttttacc gatcgctacc
     2461 tttataaacc aggtgagata gttcatgtta aagggatttt cagatatcga aacaatgacc
     2521 agtggagttt aaatccgccg ctatctccag tgaaagatat tattgtcacg gtatataatt
     2581 ctcgtcatga ggagattgaa acgtatacaa agtcactatc gccaacaggt ggggtgcagt
     2641 gggatatacc aataaagggt aactatccta caggatacta tcgtataact attacagcta
     2701 aaaataaaga ttataatatt aatggtggta ttcactttca ggttgaggag tttaagcctg
     2761 caaaagccga gatgaagatt attcctgaaa agttaaagta tcattggggt gagatgcttg
     2821 caatggatgt gtttgggtgg tacctgtttg gtgcgccggt taacaatgat gttgaatata
     2881 cagtttcgtt aacgccggtt atatacagga gtgaatcata tccagattat tcgttttcta
     2941 cacagttgta ctactatgat gaatatgatg agtacacccc atcatcaact actatagcta
     3001 caggcagggt aaaggtaaat gaaaaggggg ttgccagggt atttgttccc cttaaggcaa
     3061 tggtaaatgt tcccaatgct gatattgtta taactgcaaa gacctacctt tctgatgatt
     3121 caccggtgtt tggagctaaa gcgggattac aggtgtttga gccggttcat gttggtatac
     3181 gggtgccacg ctattttaat aataccgaca gtcctgtaac agtacaactg gtagccctga
     3241 atagcagtga taaagtggtt gaaggaaata ctgtcaagct tgtggtgaaa aagcatgagt
     3301 ggaaatcatt tcagattgcc ggtgtcaatg gcagattgca gtgggaatat aaaaatatag
     3361 cgacagatgt atatgcacac aattatgtca tcggcaaaaa ggaggtagca gtagcaataa
     3421 atgatccagg ctattatacc gccgaggtgt acgatagttc cggcaaacga aaatttgcag
     3481 tgaagagctt ttacagtata gggaagggtg aagctggctg gcgcgtcaat gacgatgaat
     3541 cggttgatgt tgaaaccgat aaaagcagtt acaatgttgg tgatactgcc atgctgcttg
     3601 ttaaaaatcc ctttgaaaag gcgtctgcca tggtaacggt tgagcgtgag aaattttttg
     3661 actattttga agtccctgct actaccagca ttgtcatgat accaataaaa ataaccgagg
     3721 aacatatacc caatatatat gtcagcgtta tggtatatac gccacgaaca ggttttaata
     3781 aggtaaagca tggagaggat ttagcaaaac caaaatgcta catagggtat gccagtataa
     3841 aggttgttcc ccgcgaaaaa aaattacagg ttaaggtaaa atcagacaag aagcaataca
     3901 aacctggtga agagacaacc atagagataa acgtaactaa ttataaggga cagccaatca
     3961 atggtgaggc aaccatttca gtagccgacc gcggtgttct taacctggtt aattacacgc
     4021 tgcccaatcc gctggacttt ttttatgctc ctcgcagtct tgctgttaca acatttgatg
     4081 tgaggcagat tattctgggg caacgatata taaaagaaaa aggagagctc attggtggtg
     4141 atggcggtat tgcctcaatg gggatgattg taccgagagc aaatatacgg tttagcgctt
     4201 attacaatgc aacagttaag atagaaaaag gagtagcccg gataaaattt aaactgcctg
     4261 ataaccttac aagctttaag tcaatggtgg tggtgcatac aaacgattca cgatttggct
     4321 atggtgaaac aacatttgag gttaaaaccc cgttaatggt actgccaaca tttccgcgtt
     4381 ttgcccgtgt tggtgatgca tttgatgctg gcgcacttgt gtttaactat tcaggtataa
     4441 aacaaacaat aacagtaagc atggagcttt ctggaggtat taaatgccag gatggaaaat
     4501 tgagcagcat caaagagatt gatcttaaca atggtcagtc aactgagctt accttcccta
     4561 ttgttgttga taatgccaat caatccacat ttgtcattaa ggcaaaggct gataagcata
     4621 ctgatgggat aaaggacagc attgccataa aagcacccaa catctatgaa acggttgcac
     4681 agtatggaag gattaccaaa gcggacgata gtgcaaaaca tactattgat atttcaaaaa
     4741 atatcattcc gtatgtttct tcggtaaatg tgttcgtttc gccatctgca ttttctgagt
     4801 taaagggaag tcttgattat cttgtagaat atccctacgg ctgtctggag cagaaggctt
     4861 ccaggatatt accactcatt ataggtgagg atattattat tcaaaaaaat ttactgaaga
     4921 taaaatcaaa agaggattta aaaaatactg tcgcaacgat attaaaagaa tttccaggct
     4981 attttaaaaa tgatggtttt tcatactggc aggacagttc atataccagt agctatctaa
     5041 cagtatatgc tacctttatt atgacaatgg caaaaaaaca agggtacacc gtagatgccg
     5101 taacctttga taaagcgtta gcaaccgtga aacggtttgc aacaaaaggg gttattttta
     5161 gcaatgagga aagctttttt tcaacctact ataaatatct aacattgagc tttgcccatt
     5221 atgttgccgc actcaatggc gttaccagcg aaaatcagct taaacttgtt tacagcgtca
     5281 tgaacagcaa atacgaggat atgatagcag gctatgcgta tctattaaaa gctgtagcgt
     5341 tgtatcctga ttccaatttt aagcagtcca tgaccagaat gatagcgcaa aaattctttt
     5401 cggcgatgcg tactgaatca ggttatgttt atttcaagga cgcaagtgat tggggatatt
     5461 tttattatga tgatgttata gtgacatcgc tcattttgca gtcgttgctg gaggcagggg
     5521 tgccatttga caatgattat aaggtggtca attatctgct caaacaacgg aaaggatatg
     5581 cttggcttaa tacgcatgaa aatgctatcg tctggtgggc acttaatacg tatttcacaa
     5641 agtatgaaaa gggaaaacca gattttgcgg taacggtatt gtttaatgct aaagaattgc
     5701 tcaatgcatt gattaccgat accagtacaa cctattccgg attgtatgca attactcatc
     5761 aggatagtgg ttcacggagt ttacagcttt caaagaaggg tgaaggcgtt ctctattata
     5821 cttttcgata tacctatatt cctgtgtacg acaaaatgat atcaaagaat atgggctttt
     5881 taataacaaa gcgtttcaag gatgtgaaaa ccggcaatga ggtaacagct tttaaaaaag
     5941 gttcagagta tctggttgaa atagaggtat acacgccaaa agagcggggg attactgtcc
     6001 tggatgatca gttgcctgca ggatttgagc cggtgaatat tacctttgcc actgaggaaa
     6061 aaatgacagc ggaaaaaagg aatgcttact atgcagattg gggcacattc aaccactatg
     6121 aacagtataa cgacagggtt gtatactatg ctgacttcat gcatactggc aatcacactg
     6181 ttgtatataa ggtgcgagca accaattcag gggtatttaa gctgccggca tgcaaggtgg
     6241 aggagatgta taatcccgag gtgtttggca ttatgtatta tactaacgct gtgcaggtgc
     6301 aatgatagct atgaagccgt tgctaactct ggaatgcaga tatcgcataa aaaaaataat
     6361 aaagctcagt agcggcgtca tctttttggc gatagttatc tatatagtgt ttccctataa
     6421 aagagattac cttgcgtcat caaataaact cccggttgtt atctatgaca ggaatgggac
     6481 ggtccttatg gaggtagcaa aaggcaaaag tggtttgtgc caggctatca gtatacaaca
     6541 acttcccgag gattttattt cattactgtt attttccgag gacaggaatt tttaccacca
     6601 ttggggtata tcgttaaaga gcattgcacg ggcactgtat caaaatggca aagctatgcg
     6661 cattgtttcc gggggatcaa ccatcaccca gcaggttgta aaatcaaaaa aagagatact
     6721 gcgcaatacg gtgttgacaa aaatgctgga gtttattgag gcggtacgca tggagcttca
     6781 ttttagcaag gatgagattc tgcaggggta catcaatgag gtgtatttag ggaataacat
     6841 ctatggcttt gaaaaggcag cccagatata ttttggtaag catataaatg aatgcaatct
     6901 gctggaggca ggttttttaa ttgcaatagt aaaagcaccg gaacgatata atccatatca
     6961 aaatcctgaa acagtggttc aaagtgcaaa gaggcttttg cgcaatgcct gtcacatggg
     7021 ctatgtaaaa atttctccct ttgaactgga ggtttatact aatagcagga ttgcattgca
     7081 gtataatgaa aagaaggtta ttgctccatt attctgcctg tatgcattat cgcaggctcg
     7141 tagattattt ccggataagg atatcacgca tatctatacc acgctggatg caaccatata
     7201 tagcaatgta ttaaccgtga tgcaaaattc aatgagcatg ctgaaggata acaatgcgct
     7261 gcatgctgcg ctggtgatga ttgacaacac aaccatggag attgttgcca tgattggaag
     7321 tgttgatttt tttgactata aaaaagggca ggttgatgct actcttataa agcgtcaggc
     7381 tgcatccacc atgaagcctt ttgcgtatgc tctggcactt gaaacaggta tgttccatac
     7441 atcaagtatt gtgccggatg tatatacgca attcccctct aaggttggaa actatatacc
     7501 aaaaaacttc agtgattcct accatggtcc ggtacgattt gcaaaggcgc tgggttgttc
     7561 atataatata cctgctgtat atgtagtaag taagattggg cttgtttctt attataatct
     7621 tttaaaaaag ataggttttg attcaataac acgctcgcca tcgtactatg gactgggttt
     7681 ggttctgggc aatgctgatg tcagcctgct ggaacttgca aatgcctata cagtttttcc
     7741 acggcaggga ttattttcaa aaactatcgc tataaaaaaa atacgagatg cgcatggcaa
     7801 ggaatataca gtgcagtctg ctggcagcgc aagggttttg aagcaacaaa catgctattt
     7861 gatttcgcat atattgtccg aatataaata taaagttgat gcctttggat tgtgctcatc
     7921 cattaatttt ccctttccct ttgcagtgaa gactggaaca tcaaaagatt tcaaagataa
     7981 ttttattgca ggatacaaca agcagtatac ctttgcggtc tgggttggaa atctatataa
     8041 tgagtcactg tgtgatcttc cggcggtatc gggggctggc attgttttaa aggatgtact
     8101 gctgtatcta tggaatgcag ggtatccatt tgaaaggttt accatgcctg aaaatataag
     8161 aaaggtaaaa atatgtacac tgtccggcat ggttgccgat acctattgtc atagtactga
     8221 ttatgagcta tattccggca ataaccagcc ttgcaataaa tgtaactggc acacaaaaaa
     8281 tgcaacaatt gtaccatcac agtttaaaga atgggcaagg aataaaaatt ataatgttgc
     8341 tcatgacgct tctgtgcaaa ttgtttttcc cgggaatggt gtggtgttta aaatagaaaa
     8401 aacagtccga aaaaatttac aggctatacc attgaaggca actgctaata gcaatattca
     8461 atggtatgtt gataatcggt atatagggga aggcagtgaa cttatatggc agttatcggc
     8521 agggcatcat gttattagca tacaggctgg caatcagcgc gatgctgtca acatcaccgt
     8581 tctggaataa tgttcatgaa ttcaatatgt cagtagtatt gtaaacaaat acaatgcgat
     8641 atcattggct atcgtttaaa aattgtgtac tggaatatac tgaatataac catataaaag
     8701 aataaaaata attgccataa aataacatat tgttatacat gtcataaata gagaaaaaaa
     8761 ggagtgatac gatgatgcat aaactgtttt accctgattc gattgctatt gtaggtttgt
     8821 cctcgcgtcc aaacaacatt ccacggctta cccttgaaaa taccttgcga tggggttata
     8881 gtggaagaat ttttggggtt aatccaaaaa gcgacgatga gcaggttgac ggtatcagga
     8941 tgtataaatc cataggagcc ttgcctgaaa ttcctgatct ggtgtatgca ctggttccgg
     9001 ctaaatttgt tccaggtatt gttgaagaat gtggtcaaaa aggtgtacag tggatggcaa
     9061 tccccagtgg tggattttct gagtatagcg aagaagggaa atcacttgca cagaaaacgg
     9121 ttgatactgc aaaaaaatat ggaattcgtt ttgttgggcc caatgggttg acggtagcta
     9181 atgttgaaaa tggattgtgt ttgccatttg ttcctatttt acgcccgcag tttggtggga
     9241 tgtcgatagt atctcaaagc ggtggtgtag cattgatgat gtggaatgtc ataatggatg
     9301 aaaatattgg catagcaaaa tttgccagta ttggtaataa attagattta gatgaggtgg
     9361 attttctgga atatttaggc tctgaccccg ccacatcaat tatatgtatg taccttgaaa
     9421 gtattccacg aggttctgat tttataaaag ttgctgatag gataaataaa ccaatagttg
     9481 tatataaatc gaacacaacg caggctggaa aaaaagctgc aatgagtcat acagctgcta
     9541 tgagtaatga tgaggatatt attaacgctg catttgaaaa ggccggtatt atccgtatat
     9601 acaattacca tgactttttt gctgtggcca aagcatttaa attgccacca atgaagggga
     9661 agcgtgtgat ggtgatgagc ccagctggtg gttttagtgt tatgactgca gatttatgtg
     9721 aacaggcagg atttaaattt gcaaatcccg gcaaagaatt ttatgaaagc ttgcagaaat
     9781 tttccaatgc tggtgttata cagttttcaa atcctcttga tatgggcgat atttatgatc
     9841 ccgcttttac cgcgcatgta ctttattcgg ttatgcataa caataatgtt gatggtgcca
     9901 tgtacgtaag ccagcgtcca cagatgcccg acaggaaaaa tgtattttca cagatgtttt
     9961 tatccgacct ttcaaaagaa gcatggggtg ccattgtttc ttcggggaaa ccactggcaa
    10021 tttgcctgtt aggtatggca aagttgatgg cacagataaa acgctcggta aacttcccaa
    10081 tatttaacag tgccgaggag atggtgcgtg caatgaaatt ccagatgaaa tactggttgc
    10141 gacgttctac acctcaaatg catgagtcat acccaatgag ccttgatact gtattacgat
    10201 ggataaccaa tcaccatggc gatattggtg aagatgccat ggaactactt gagttcattg
    10261 atattcccgt gccacagtca gggatagcaa agtcggaaaa ggaagcggta acagtagcaa
    10321 aacaacttgg ctatcctgtt gtatgcaagg tcatttcccg cgatatttta cacaagtcag
    10381 atgttggtgg ggtgatagtt aatgtgaatg gagatgaagg tgtaaagcag gcttatgcag
    10441 caatacaatc cagtgtacta tcgcataaac ctgatgcaca cattgaaggt atacgcattt
    10501 caacaatggc acctgatgga gtggatatgt ttgtggggag caaatatgat cagtcatttg
    10561 gacaggttat tatctttggt tttggtggca tatatgttga ggtgtttaaa gacatagcct
    10621 attgtttatg cccggctaca aaggaagaaa ttaagcaaaa gcttacttca ttaaaatcgt
    10681 atgcactgct taaaggtgcg cgtggcaaaa gtgcactgga tgttgatgcc tatgttgaca
    10741 ttatatggaa ggtatcacat cttttagcca gtgcatccca tattaaagag ctggacctta
    10801 atcctgtgcg ggtgtttgaa cacggctgtc aggttttaga tgtgaggatg agaatagttc
    10861 attaaatttt aatttgcagg ttgttgtggc actgctatac ttatggctat ggtatatctt
    10921 gataatgcag caaccagttt tcccaaaccg gatacggtaa taaaggctgt aaccaattgc
    10981 ttaacaaact actgtgctaa tccaggccgt tcagggcatt cactttccat tgaagcagcg
    11041 cggaaagtat ttgaaacgcg tgaaactgtt gccgaattgt ttcatgcaaa agattcacgg
    11101 tatgtcatct ttactgccaa tgccacaatg gcaatcaatc tggtttttca tgcacttttg
    11161 caaaaaggcg atcatgtcgt cgtctcatca atggaacaca atgcagtgat gcgtcctctc
    11221 agacattggg aatcacaggg tatcatcaca ctttctttgg caaatgccga tggcaatggt
    11281 ttcgttgatg atgacattgg tgctttcata cagccagcca ccagattatg tgttattaat
    11341 catgcatcca atgttaatgg tgtcgtacag gatatagcat ctattgcagg cagaataaaa
    11401 gaaatcaatc ctgatgttgc agtgcttatt gatgcttcac agagcaccgg gcttatacct
    11461 attgatatac aggcaatcaa atgtgatttt gtagcgttta ccgggcataa agcattatat
    11521 ggacctcagg ggattggtgg acttgtaata aatacagcaa agcacttaca gccgtttatc
    11581 atgggtggta ccggcagtaa atctgaaagc caattccaac cgcagttttt acccgataaa
    11641 tttgaaagtg gtacgcttaa tcttccgggt attgctggac ttaaagcagg gattgactat
    11701 gtaaaatccc gtatggatga aatacaccat aatgaactat tgcatataaa aaccatagtt
    11761 gaagggttgt catccattaa agatttgaag cttttctgga gcagatcgtt gcataatcag
    11821 attggtgtta tatcagtagc tcataatcat ataccctgca gcgagattgg acaaaggctc
    11881 aatgatgaat tttcagtttt tgtacgcacc ggtttgcatt gtgcaccgct ggctcatgaa
    11941 acactgggta cctatcctaa tgggacgtta cgggtgtcag taggtttatt taccaacaaa
    12001 agtgatgtcg atgtattttg caaagcaata gaaatcattt caaagtgaag aatatggact
    12061 attgttattt attattaccg tcagtacatg atgcaatgaa agctgagaag cttttattac
    12121 aatcaggttg tgcaatacag gttattgcca ccccgcggga aatatcgcat aactgcgggg
    12181 tggttataaa atttagctgt gctgaaatca atacggtact actatcttgt aaggaactgt
    12241 cggcagaaca aaagatattc aaaaaaaata gtaataatgt gtattacgaa attatcccag
    12301 agtagattct aacttcctga tgctttcagc aagtatctta tactcttttg aattttttgg
    12361 aaatgtgctc tggtactttt taaagtactt tatctgttcc atcttccact gtttcatatc
    12421 aaagctgttt tgaatcataa ataatccccc ttgtacaata ctgtactata attgtcgtaa
    12481 aaatgatcta ttccattgag aagaaaattt ttcgcctgca ttttttcaac actgcaaggc
    12541 attctatggc ttacaatgcc ttcacaataa ctatcgtaca tgcatcatat actgggcgat
    12601 agttactatg tattatgtag tgttttgcaa ataagtgcaa gctacagcag gctatatttt
    12661 tttgattacc ggtgttattg atgtactctg atgaagatat atttttttac aataaaaaaa
    12721 agctgctcaa atcagagcag ctttttttat taaaatgtat tcgtaattac cacttttcaa
    12781 ctaattcaaa caatgcagca gcacccatac cgccgccgat acacattgac tcaataccgt
    12841 atttaata
//
LOCUS       DAOSOX010000056        12754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_4913_length_12754_cov_7.998740, whole genome shotgun sequence.
ACCESSION   DAOSOX010000056 DAOSOX010000000
VERSION     DAOSOX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4913_length_12754_cov_7.998740"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLX22_05775"
     CDS             <1..381
                     /locus_tag="PLX22_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19440.1"
                     /translation="EHNIKPLLHKGYVVIVDRYFYSTAAYQAGDGNSPHEIVRMNFEK
                     GFPVPDRVYFIDIEPEIALTRIAMRSGNNRDIFEKLSELTRIRSNYLSIADDRFCIIN
                     GNNSKEAIFDAIQKDFIQHFSDVT"
     gene            389..1684
                     /locus_tag="PLX22_05780"
     CDS             389..1684
                     /locus_tag="PLX22_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT19441.1"
                     /translation="MKLLRQLIIQCFNVCFIAILLLMGWMLYNVHIAGDLLIIFLIIV
                     TVTVFPLYTYWIEHIHKKILPFRYEDLYLQTVDMFLSLQSYDDIIKVIFERVLRFIGV
                     RSGLLIFYSHATDDYTIYYQKQQKQKIIRKARIDKNNIILKIIQSGDDILIKSKLDPS
                     LHFQRSIIFEMEKLGSEIIIPIYYHDIFLGLMVIGEKKTRISRKDILMLKALASKIAT
                     VTVNSFFMHELIKNKEIEKEYELSFKVLKKFLPPDEGFIHNTHYRVIRHPGNEAHYFF
                     SIYNCSDSISYVIICSLRANIPLLSLLIPAISVLFQSYARLGYYPLTIIEKINAVIKK
                     KELIEKDLSLLVLKKCNNNYFIGKSRTSDIKLFVYNGSTVKPVMSNDDSAMIRNPVRL
                     IIARHDMAAIKKNPELLDNTVSLSNTIGENEFIMILTRA"
     gene            1684..3996
                     /locus_tag="PLX22_05785"
     CDS             1684..3996
                     /locus_tag="PLX22_05785"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT19442.1"
                     /translation="MKKHYRIYACIVFYIVFFILIVPQSYHNALQYTFDRLVIGKTFY
                     YPISSFPKAMFQYHIISCRQLATTSNDDAGLECTMAGNNEMLQQQFTNNNRTAVWIVL
                     IVSISSIVIVLWGIGVYLARHDIFSQLFMIVAVSIALCLLMYIDVFFTGKLFIMFPVV
                     NILVGWSLLHVTYTINDYSKHGKKVWYTAAACIALVLSIISLVSDIAFETIYMLTIIM
                     TLLLFLASIMVLTVTINHKLSFVLRRNLVIAAAIFLGGVVPYAILLTGTIADMYPGIL
                     FALFFAIATPVSAGNGLLASSSLASMANLQKNFIAMVMDIIIAVVLAFSLFYIYNFYS
                     ILSGMYYGIIIFVIAMIILLHLRMLIVRTFDDIIFLNNDEYALSLQRIAEIITSPRSL
                     KDKLDSIQSELHTLIRVEGIQYAIIDDYEYGDSRIINVKSDSDIVTFFKKRKETITMT
                     SSFTNNEYERALQRYCKHDHISSCIPVYIDGILQGVLLIKGKEGDYFTNPELSFLAML
                     SLQVHQLIVNSKALAEYIASRDYETEMDLASYIQLRLFPKKSPVDCGIQFAIYARPYL
                     KVTGDYYDIVTIDKNKVAVVIADISGHGLSAAMILSATSAIINGMLKEKKNISNVVAE
                     LNHFLTMRYQGFELITLFIGLFNKKTRSMEYINAGHIAPVVLSHDKKFYRLEGRSKIL
                     GVDPAAQFHASRYSFKAGDEMILYTDGLTDLYDARNDVSFGDEGLEEVLQQTIMKPIE
                     EKIHAITEAIEAFGQEHIKDDITIVGMYID"
     gene            3914..6412
                     /gene="sppA"
                     /locus_tag="PLX22_05790"
     CDS             3914..6412
                     /gene="sppA"
                     /locus_tag="PLX22_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT19443.1"
                     /translation="MQLLKQLRLLDRNISKMILPSLACILISIFWSNLAYAEMKVQPS
                     DGVFPGVTASVPSLPAYSYSNPVYADINEGFSLQYRYAYIDPVGVGTHYVATTFYGFT
                     VGYNTLEIEIDDKEITRTDGNSWYFAKGIMLTDWFGFGILYNRYTFDGETVKAWGYSF
                     LLQPSKYLSFGCVIDHANEPSPGLLSLRQREWYSMSLRPFGNMFSLSWDIKRYEGEEW
                     GDVLHIFTLSGMMWHDILYAVQYDSDKNISFSLAVPFDISTRTGASSIYDFGYTNFNK
                     QESHAYTFGITLTDERFRNAIGPKQRFLSIVLQEPVKEIKERGIFERSRASFFEIVRS
                     LREATYDESIKGVVLQIDAPVLTFAQAQEIREEIKRCKGNGKPVYCIVTSVGNLEYYV
                     ASSATKIYYTPNQPFGITGLKAEVYFFKGLLDKVGIRFEEVKKGQYKSFGESFTRQHM
                     SDAARENLIEVLKDLNQQFISDIASDRKLTVEDVEKCMAGGILTPQQAQAQGFVDVVA
                     RPMEVLQSLGVERYGLSYMVQCDDYVEEKMYAYDWGPKPQIAVIYVEGNIIRGTAKDN
                     NFLSPAMIGDVNYKKFLEKVFADSSVKAVVIRINSGGGSAIASDLMWHSLVALKKRYN
                     KPVIISFGNMAASGGYYIACTGDTIFTSKSTITGSIGVLFGKLNLEELYAKLGISKET
                     IKMSEFADIFSESRAMTEKEKQLIQKNVDFTYNEFIQKVEMGRNIPADAIPQVAEGKI
                     FTGSQALNNGLADTQGGLIVALSYAASLANIKGEFKVIQLPEKDIDLRKILKGTSFQT
                     ALSDMMQYMYNMLIFESLNKEEVLLLYPYEIVIK"
     gene            6475..7956
                     /locus_tag="PLX22_05795"
     CDS             6475..7956
                     /locus_tag="PLX22_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19444.1"
                     /translation="MKRVSMKKLQLFLCMVFILASTVLYAKPKAGFGLIRGFSSEEMF
                     LATLLRQHIINIAASLQIFDSVNPENLARQQQRYACYDEQCMLLFTREMGLSLFVIGT
                     VSQTASGYRVHIAAYGLDIPYNGKEVCSYTATVNVTIMDKSDREASYIAEEIASQFIV
                     LLCQRLQVPAYISHNTVKSDFTINGTFTCYTVETLHDDFVSLKPMDNVTIVNNIVAGN
                     GVYDDCMVLIDFKKEAEAIQQFYYGRKQEIVFKKAAWDDSCSLIAFTIPASATMPVVV
                     PFFGYYASEDYEGLLLWIGNVVPYTGIQMWGFTHKPDTLKKHHEDISRHDRALYYFGW
                     YMLFVGGMPSFVDAFSHSYLEQARYYKGNVPTMGDKKNEVYLSLLGGGSGMFYKGYRA
                     WGYWYFHITNLCVYGLLYNYARPSEWDSANHRYRTHDANKSASYAFLSLLSVIKIVEM
                     VHTLSVPYTISVVEQEDARIEVLPVFAVNDYEKITGIACAMHF"
     gene            7938..9377
                     /locus_tag="PLX22_05800"
     CDS             7938..9377
                     /locus_tag="PLX22_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19445.1"
                     /translation="MRNAFLIVWCMVYSVIGTAAYADDIPIGVIIVNTSVDTPMIKAL
                     GSITSEAIKEAFKNGRHLVPYDAQTFLQITNTKSADEIVGKAHLYGVSLIVYMQVFLV
                     GPVYTCNLTFKPLEAMSTFKEETISIRSTIAKNIPLKAAKEILKRHSDVLPVTIKQKI
                     NNYTYIINAGQWHGLQQGRYTLTDGAECAIVSVKRYSALVTIHADYNEAQQITMSVGN
                     KITKLIKRIDAQIHENINQEYSGKKLLKGDSDGKRAIEATCVVNPSGNILLPGYGAFL
                     STHYLGFSHSEPDWPGIYVALGAVATQLMLVPALGNWDVNFFPWVNGNKTNAQYRLGI
                     YLWATLPVTYTVAFFNQLSYAYQKQKVLPPFFLHDDVTAFVASAIVPGGGLFYKGYRL
                     WGHAYWLTECTLGGLLTYHWEDGGKRAVFAGALIGIKLIELIHAVLASPAYSVYTYEL
                     SNNENVHVDVGMAPFQNEPCFYAYFFKNY"
     gene            9431..10516
                     /gene="carA"
                     /locus_tag="PLX22_05805"
     CDS             9431..10516
                     /gene="carA"
                     /locus_tag="PLX22_05805"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOT19446.1"
                     /translation="MQKRAYLLLEDGTIFEGYSFGYEKDAIGEAVFNTSMSGYQEVLT
                     DPSYSGQIVAMTYPMIGNYGVNNDDVESSKVQVAGFVVKEYSKAYSNHRAIRSLDEYL
                     KDYKIPGIEGIDTRRLTRHIRDKGAMRAGICMDTSGAMERILAHPAMAGLDLTCNVMC
                     NKPYSFGKDRSEAPTIAVFDFGVKMNILRLLHEQGCNVVVYPGNMALSQVLKDRDIKG
                     VFLSNGPGDPDAVEYAKILVKDIMKAHIPCFGICLGHQLIALGMGAKTYKLKFGHRGT
                     NQPVKNLLTQKIEITSQNHGFAVDYESIKHNSDVEITHINCNDTTVEGLRHKKLPLFS
                     VQYHPEANPGPHDSRYLFQQFYELVTH"
     gene            11136..>12754
                     /locus_tag="PLX22_05810"
     CDS             11136..>12754
                     /locus_tag="PLX22_05810"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOT19447.1"
                     /translation="MDIWNDIVIDSDREPNLTNNAQIVLEKRYLAKDEEGKVIETPRQ
                     MFERIAKYIASADFLYGRDSKHVSQVATTFYDMMVDLLFIPNSPTLMNAGRPLGQLSA
                     CFVLPVEDSMDAIFDAIKYMALIHKSGGGTGFSFSKLRPKNDVVQTTKGVSSGPVSFM
                     NIFNTATETIKQGGTRRGANMAILHVTHPDILEFIKAKEKNDALTNFNCSVALSDAFM
                     NAVENDDDYELINPRNNRVSGTLKAREVFNLIVRMAWKNGEPGIVFIDRINEKNVLKK
                     VGLIESTNPCGEQPLLPYESCNLGSINVARCVVNNGKSVRVDYELLNDITYKAVHFLD
                     NVIDVNNYPLPIIGEKTKANRKIGLGIMGFADMLIALGIPYDSEAALGVAKNIMSAIQ
                     DASKEASRALAKERGAFPNFPLSEYAHNGDEPLRNATTTTIAPTGTISIIAGTSSGVE
                     PIFALAYVRNVLDNSRLIESNPLFVEYCKERGLYTDELMQKVAQTGSVAHIEELPEDV
                     RALFVTAHDIAPEWHVRIQAAFQEYVDNAVS"
BASE COUNT         3885 a         2169 c         2802 g         3898 t
ORIGIN      
        1 gagcacaaca tcaagccttt gttgcataaa ggatatgtgg tgatagttga tagatatttt
       61 tattcaacag cagcgtatca ggcaggtgat ggtaacagcc ctcatgaaat tgtccgcatg
      121 aattttgaaa aaggttttcc ggtccccgac agagtatatt ttattgatat tgaaccggag
      181 attgctctta caaggattgc tatgcgatca gggaataatc gggacatttt tgaaaaatta
      241 tcagagctaa caagaatccg cagtaactat ctgtcaatag ccgatgacag gttttgtatt
      301 atcaacggca ataacagtaa agaagcaatt tttgatgcaa tacaaaaaga ttttatacaa
      361 cattttagtg atgtaacgta acaataccat gaaattatta cgtcagctga taattcaatg
      421 ttttaatgtt tgttttattg ccattctttt attaatggga tggatgcttt ataatgtcca
      481 tattgctggt gatctgctta tcatctttct tattattgtt acagttactg ttttcccgct
      541 gtatacctat tggatagaac atatccataa aaaaattttg cctttcaggt atgaagatct
      601 ttatttacaa accgttgata tgtttttatc gttacaatcg tatgatgata ttataaaagt
      661 tatatttgaa agggtgttgc gctttatagg ggtgcgctca ggattattaa tattttattc
      721 acatgccacc gacgactaca ccatatacta tcaaaaacag caaaaacaga agataatccg
      781 gaaagcccgc attgataaaa ataatattat attgaagatc atccagtctg gtgatgatat
      841 actgataaag agtaagcttg atccctcgtt gcattttcaa cgttctatta tctttgaaat
      901 ggaaaagctt ggcagtgaga tcatcattcc gatatattat catgatatat ttttaggact
      961 tatggttatt ggtgaaaaga aaaccaggat ttccagaaag gatatattga tgttgaaggc
     1021 acttgcatca aagattgcga cagttactgt taatagtttt tttatgcatg aactgataaa
     1081 aaataaagag atagaaaaag agtatgaact atcattcaaa gtattgaaaa agtttttgcc
     1141 gcctgacgaa ggtttcatac acaatactca ttacagggta atacgccatc ccggaaatga
     1201 ggcgcattat ttctttagca tctacaattg tagcgatagt atatcgtatg tcatcatctg
     1261 ctcattaaga gcaaatatac cgttgctatc gctgcttata cctgccatta gtgttttatt
     1321 ccagagttac gcacgtttag gttattatcc ccttactatt atagaaaaga tcaatgccgt
     1381 aataaagaaa aaggaactta ttgaaaagga tctttctttg ctggtactaa agaaatgcaa
     1441 caataattat ttcattggta aatcacgcac ttcagatata aaactttttg tttataatgg
     1501 atctaccgtt aaacctgtta tgagtaatga tgacagtgct atgataagaa atcctgtacg
     1561 tttgattata gccagacatg acatggcagc aatcaaaaaa aatccggaat tgctggataa
     1621 tacagtatcg ttaagcaaca ccataggtga aaatgaattt attatgatac ttacgagagc
     1681 ataatgaaga aacattatag aatatatgct tgcattgttt tttatattgt tttttttata
     1741 ctgatagttc cacaaagcta tcacaatgca ttgcagtata cctttgatcg gctggtaatt
     1801 gggaaaacgt tctattatcc aatttcatcg tttcctaaag ctatgtttca atatcatatc
     1861 atttcgtgca ggcaattggc cactaccagt aatgatgacg ctggccttga gtgtaccatg
     1921 gcagggaata atgagatgct gcagcagcaa tttactaata ataacaggac tgcagtatgg
     1981 atagtattga ttgttagtat ttcatcgatt gtgatagtgc tatggggaat aggggtttac
     2041 ctggcacggc atgatatatt ttcacagtta tttatgattg tagccgtgtc tattgcttta
     2101 tgtttattaa tgtatataga tgtttttttt acagggaagt tgttcatcat gttcccggtg
     2161 gtcaatattc tggtgggatg gtcgttgctg catgtaacat atactatcaa tgattatagt
     2221 aagcatggta aaaaagtgtg gtataccgct gctgcctgca tagctctagt gctaagcatt
     2281 atatcattag ttagcgatat tgcttttgaa acaatataca tgcttactat catcatgact
     2341 ctactgttat ttcttgcttc gattatggtt ttgacggtta ccattaacca taagctatca
     2401 tttgtattac gacgaaatct tgtcattgct gctgctattt ttcttggagg tgtagtgccc
     2461 tatgcaattt tattaacagg aactatcgcc gatatgtatc ctggcatatt atttgctctt
     2521 ttttttgcaa ttgccacccc tgtcagtgct ggcaatggac ttcttgcaag ctcaagtctt
     2581 gcctccatgg caaatctgca aaaaaacttt atagcaatgg taatggacat tattattgct
     2641 gttgtgctgg cattcagttt attttatata tataattttt attccatttt atccggcatg
     2701 tattatggta tcattatttt tgttattgcc atgattattt tgttgcacct gaggatgctt
     2761 attgtacgga cttttgacga tatcatattt ttaaacaatg atgaatatgc actatcctta
     2821 caaagaattg ccgagattat tacatcgcca agaagtttga aggataaatt agattcaata
     2881 cagtcagagt tgcatacctt aatcagggtt gaagggatac agtatgccat cattgatgat
     2941 tatgagtatg gtgatagtag aattataaat gtaaaatctg actctgatat agttaccttt
     3001 tttaaaaaaa gaaaagaaac catcacaatg acaagttctt ttaccaataa tgaatatgaa
     3061 agggcattgc aaaggtattg taaacatgat cacatatcat catgtattcc agtttatatt
     3121 gatgggattt tacagggggt tcttttaatc aaaggaaaag aaggtgatta ttttacaaac
     3181 cctgagctaa gttttttagc aatgttgtct ttgcaggtac accagttgat agttaattct
     3241 aaagctttag ccgaatatat tgcatcacgg gactatgaaa cggaaatgga ccttgcatca
     3301 tatatacagt tgaggctctt ccccaaaaaa tcacctgttg attgtggcat tcagtttgca
     3361 atatatgcac ggccatacct taaggtaacc ggtgattatt atgatattgt tactattgac
     3421 aaaaataagg tggcggtggt cattgctgat atttcagggc atggtttgtc agcagcaatg
     3481 atattatcgg caacaagcgc catcattaat ggtatgttaa aagaaaaaaa gaacattagc
     3541 aatgttgttg cagaattaaa tcatttcctt accatgcgct atcaggggtt tgaactcata
     3601 acattattta ttggactttt taataagaag acacgatcaa tggaatatat taatgcaggt
     3661 catatagctc cggtggtact atcacacgat aagaaatttt atagattgga agggaggtca
     3721 aagattttag gggttgatcc tgcggcacaa tttcatgcat cacggtactc tttcaaagct
     3781 ggtgacgaaa tgatactgta taccgatggg ttgacagatt tatacgacgc cagaaatgat
     3841 gtatcttttg gggatgaagg attggaagaa gttttgcaac aaacaattat gaaacctatt
     3901 gaggagaaga tccatgcaat tactgaagca attgaggctt ttggacagga acatatcaaa
     3961 gatgatatta ccatcgttgg catgtatatt gattagcata ttctggagca atttggctta
     4021 tgcagaaatg aaggtgcagc catccgatgg tgtattccct ggtgttaccg catcggtacc
     4081 ttcgttgccg gcatatagct atagcaatcc agtgtatgct gatattaatg aaggattttc
     4141 tttgcagtat cgatatgcgt atatagaccc ggtaggtgtt ggaactcact atgttgcaac
     4201 tacattttac ggctttaccg ttgggtataa tacactggag atagaaattg atgataaaga
     4261 aattaccagg actgatggca attcatggta ttttgcaaaa ggcattatgc ttacagactg
     4321 gtttggtttt ggaatattat ataatagata tacttttgat ggtgaaacgg tcaaagcgtg
     4381 gggatatagc ttcctgctgc aaccttcaaa gtatctatca tttgggtgtg ttatagatca
     4441 tgcaaatgag ccatcacctg gtttgctttc cttacggcag cgtgaatggt attcaatgag
     4501 ccttcgtcct tttgggaata tgttttcgct ttcatgggat atcaaacgat atgaaggtga
     4561 ggaatggggc gatgtgttgc atatatttac cctctcaggg atgatgtggc atgatatcct
     4621 gtatgctgta cagtatgatt cagataaaaa tatttctttt tcactggctg tcccctttga
     4681 catttcaacc agaacaggcg cgtcatctat ctatgatttt gggtatacta attttaacaa
     4741 acaggagtca catgcgtata cgtttggcat tacccttact gatgaacgat ttagaaatgc
     4801 aataggtcca aaacaaaggt ttttgtcgat agttttacag gagcctgtta aagagataaa
     4861 agaaagaggg atatttgagc gctcacgagc atccttcttt gaaattgtcc gttcattgcg
     4921 cgaggcaacg tatgatgaaa gtataaaagg tgtagtacta cagatagatg cgccggtgct
     4981 aacgtttgca caggcacagg aaattcgtga agagataaaa cggtgtaaag gaaatgggaa
     5041 acccgtatat tgtattgtaa cgtcagtggg taatttagaa tattacgtag catcgtctgc
     5101 aaccaaaatt tactatactc ccaaccagcc atttggtatt accggactaa aggctgaggt
     5161 gtattttttt aaaggtcttc tggataaggt tggtatcagg tttgaagagg tcaaaaaagg
     5221 tcagtataaa tcatttggcg agtcatttac ccgccagcac atgtcagatg cagcacgtga
     5281 aaatcttata gaagtattga aagaccttaa ccagcagttt ataagcgata ttgccagtga
     5341 ccgaaagctg accgtagaag atgttgaaaa gtgcatggca ggtggcattt tgacaccgca
     5401 gcaggctcaa gcacaggggt ttgttgatgt agttgcccgt ccaatggaag tgttgcagag
     5461 ccttggcgtg gagcgatatg gcttatcata catggtgcag tgtgacgact atgtagaaga
     5521 aaagatgtat gcatatgatt ggggtccaaa acctcagatt gcagttatct atgttgaagg
     5581 aaatattata cgtggaactg ctaaggataa taattttcta tcgccagcta tgattggtga
     5641 tgtaaactat aaaaaatttt tagagaaggt ttttgccgac agctcagtta aggcggttgt
     5701 aattcgtata aactcgggtg gtggttcggc cattgcatct gatttgatgt ggcattcgct
     5761 tgtagcattg aaaaaacgct ataataaacc ggtgatcatc tcgtttggca atatggcagc
     5821 ctcaggtggg tactacattg cctgtacagg cgataccata tttaccagta aatcaactat
     5881 caccggctca attggtgtac tattcggcaa gctgaacctt gaagagttat atgcaaaatt
     5941 aggcataagc aaagagacaa tcaaaatgag tgagtttgct gacatctttt ctgaatccag
     6001 ggcaatgacc gagaaggaaa agcagcttat acaaaagaat gttgatttta cctataatga
     6061 atttatccag aaggtggaga tgggcaggaa tataccagcc gacgctattc cacaggttgc
     6121 agaagggaaa atttttactg gttcgcaagc tctgaacaat ggacttgctg acacacaggg
     6181 cgggcttatc gttgcgttgt cgtatgcagc aagtttagca aatataaaag gtgagtttaa
     6241 ggttatacag ttaccggaaa aggatattga tttacgaaaa atcttgaagg gaacaagttt
     6301 tcagacagct ctttctgata tgatgcagta tatgtacaat atgctgattt ttgaatctct
     6361 caataaagaa gaggtgttgt tactgtatcc ttatgagata gttattaagt aggggcaggg
     6421 tgttttccca tcttatccat attctcaata aaaatctttg ctggcagggc atggatgaaa
     6481 cgagttagca tgaagaaatt acagttattc ctgtgtatgg tgtttattct tgcttcaacc
     6541 gtgctgtatg caaaaccaaa ggcaggattt gggcttattc gtggtttttc ctcagaggag
     6601 atgtttctgg caacccttct gcggcaacat attatcaaca tagcagcttc attgcagatt
     6661 tttgatagtg ttaatcctga aaatttagca cggcaacagc agcgctatgc atgttatgat
     6721 gagcagtgta tgctactttt taccagagag atgggactgt cgttatttgt cattggtaca
     6781 gtaagccaga cagcaagcgg ctaccgggtg catatcgcgg catatgggct ggatataccc
     6841 tataatggca aagaggtatg tagctataca gcaactgtca atgtcactat catggataaa
     6901 tccgatcggg aggcaagcta tatagccgaa gagatagctt cgcaatttat tgttttatta
     6961 tgccagcgat tgcaggtgcc ggcctatata tcgcataata ctgtgaaaag cgattttacc
     7021 atcaatggaa catttacctg ctataccgtg gagacactgc atgatgattt tgtttccctt
     7081 aaacctatgg ataacgttac catagtcaac aatatcgttg ctggtaatgg tgtatacgac
     7141 gattgtatgg tactcattga ttttaaaaaa gaagcagaag ccatacaaca attttattat
     7201 ggccgaaaac aggagattgt ttttaaaaaa gcagcatggg atgatagctg ttctttaatt
     7261 gcttttacta ttccggcaag tgctaccatg ccggtggttg taccgttttt tggatattat
     7321 gccagtgaag attatgaagg attgctgtta tggattggca atgttgtccc ctatactgga
     7381 attcagatgt gggggtttac ccacaaacct gatacattga aaaaacatca tgaggatatc
     7441 tcgcgtcacg atagagctct atactatttt ggctggtata tgctctttgt tgggggcatg
     7501 ccttcgtttg tcgatgcatt ttctcattca taccttgagc aagcacggta ctataagggt
     7561 aatgtcccca ccatgggcga taaaaaaaat gaggtttacc tgtcactgct tggcggagga
     7621 agcggcatgt tttataaggg atatcgagca tggggatact ggtattttca tattaccaat
     7681 ctttgtgttt atggattact ttataattat gccagaccgt cagaatggga cagtgcaaat
     7741 cataggtata gaacacatga tgcaaataaa agcgcatcat acgctttttt aagcttgctg
     7801 tctgtcatta agatagttga gatggttcat acattatccg tgccgtatac tatatcagtt
     7861 gtggagcagg aagatgcacg cattgaagtg ttgccggttt ttgcagtcaa cgattatgaa
     7921 aaaattacag gtatagcatg cgcaatgcat ttttaatagt atggtgcatg gtatatagtg
     7981 ttatcggcac ggcagcctat gctgatgata taccaatagg tgttattatc gttaatacca
     8041 gtgttgacac ccccatgata aaagctctgg gcagtattac atcagaagca atcaaggaag
     8101 catttaaaaa cggcaggcat ctggtgccct atgatgcgca aacattttta cagataacaa
     8161 acactaaaag tgctgatgaa attgttggga aggctcatct gtatggtgtt tcccttattg
     8221 tgtatatgca ggtatttctt gttggacctg tttacacctg caacctgaca tttaaacctc
     8281 ttgaagctat gagtactttt aaagaagaaa ccataagcat acgcagcacc atagcaaaaa
     8341 atattcctct gaaagcggca aaggaaattt taaaacgcca ttctgacgtt ttaccggtaa
     8401 ctatcaaaca aaaaataaat aactatacct atatcataaa tgcagggcaa tggcatggat
     8461 tacagcaggg acggtatacg cttacagatg gagctgaatg cgctattgtg agcgttaagc
     8521 gttatagcgc tctggttacc atacatgccg attacaacga agctcaacaa attaccatga
     8581 gtgttggaaa taaaattact aagcttatta aacgtattga tgctcagatt catgagaaca
     8641 tcaatcaaga atattcgggt aaaaaactgc tgaaaggtga cagcgatggt aagcgcgcga
     8701 tagaggcaac ctgtgttgtt aatccttctg gcaatatact gctgccgggg tatggagcat
     8761 tcctttcaac acactatctg ggattttcgc acagcgaacc cgattggccg gggatatatg
     8821 ttgcgttagg tgcggttgca acacagctta tgcttgtccc ggcacttggc aactgggatg
     8881 tcaatttttt cccatgggtt aacggcaaca aaacaaatgc tcaataccgc ctgggcattt
     8941 acctgtgggc aacgcttcct gtcacctaca cggttgcttt ttttaatcag ctttcgtatg
     9001 cgtaccagaa acaaaaggtt ttgccaccat tttttttaca tgatgatgtt acggcttttg
     9061 ttgcttcggc gatagttcct ggtggaggtc ttttttataa agggtacagg ctgtggggac
     9121 atgcttactg gcttacggaa tgtacgttag gcgggttgct gacctatcac tgggaggatg
     9181 gcggaaagcg ggctgttttt gcaggagcct taatcgggat aaagctcata gagctgattc
     9241 atgcagtgct tgcttcgcca gcatattcgg tatataccta tgaactatca aacaatgaaa
     9301 atgtgcatgt tgatgttggt atggcaccgt ttcaaaacga accatgtttc tatgcatatt
     9361 tttttaaaaa ttattgaatc acagcgagcg tgctatataa ttgttaaaca gctaaaaaca
     9421 aaggatgcct atgcaaaaaa gagcatattt actgttagag gatggaacca tttttgaagg
     9481 gtatagcttt ggctatgaaa aagatgcaat aggcgaggct gtattcaaca catcgatgag
     9541 cggatatcag gaggttttaa ccgatccttc atatagcgga cagatagttg ccatgaccta
     9601 tccaatgatt ggtaattatg gtgttaacaa tgacgatgtg gagtcttcta aagtgcaggt
     9661 ggcaggattt gtggtcaaag agtacagcaa ggcatattcc aatcaccggg ctattcgctc
     9721 acttgatgaa tatcttaaag attataaaat acctggcatt gaaggaattg atacgcgcag
     9781 attaacgcgc catattcgcg ataaaggtgc catgcgtgca ggaatatgta tggacaccag
     9841 tggagccatg gaaaggattt tagctcatcc tgctatggca ggattggatt taacctgtaa
     9901 tgtgatgtgt aataaaccgt actcatttgg taaagataga agtgaagcgc caaccattgc
     9961 cgtatttgac tttggtgtaa agatgaatat tctacggctg ctccatgaac agggatgtaa
    10021 tgttgtggtg tatcccggga atatggcgtt atcgcaggtt ttaaaggata gggatatcaa
    10081 aggggtgttt ttatccaatg gtcctggcga ccctgatgct gtggagtatg caaaaatcct
    10141 ggtaaaagat ataatgaagg ctcacatacc ctgttttggg atatgtctgg gacatcagct
    10201 tatagcattg gggatgggag caaaaaccta taagcttaag tttggacacc ggggcacaaa
    10261 ccagccggta aagaatcttt taacacaaaa gattgagatt acatcgcaga accatggatt
    10321 tgctgttgat tatgaatcaa taaaacacaa cagcgatgtg gagataacgc atattaactg
    10381 caatgataca actgttgagg gattgcggca taaaaagctg ccactattca gcgttcagta
    10441 tcatcccgag gctaatcccg gccctcatga ttcgcgctac ctgtttcagc aattttatga
    10501 attggtgaca cactagctgc aaggtagaga agcaatctat tcatgagtgc aaactttttc
    10561 aggataattt ttttatctga tgaatcatcg ataaaaaatg ttattactgc gttatagtaa
    10621 caggatagta gatagtaaag aataatcttt ttcatcttcc tttaacggca tgtacataca
    10681 ttgcaaatga tgatcattat tatactggat gctttcatac ttacattttt tatgatattg
    10741 tatgggcgca ggtacgggag aaatagtgta ttgtagcgct accaatgatg tgaataatga
    10801 gaattctgat ttttccatgt gcccataaaa aagaattgct tctttcaaaa tgattcacaa
    10861 gtttggtatt tgattaatgc cggataattg tatatactct atatagtatt tattttttag
    10921 ttaaactact gatggctgga ttgtgaacaa ccatagagaa tctcatggtg gggaaggatt
    10981 ccggaacatg tccggaatga cggaatgacg agagcggcaa atgtgttccg ggttaagccc
    11041 ggaacgacga tacagaaaac gtgaatatgg attaagccag taatgacaat ggcaaataat
    11101 tatatgttca atggaggttt tttgtgagta caaagatgga tatctggaat gatatcgtta
    11161 ttgattcgga tagggaacca aatcttacaa acaatgcaca gattgtattg gagaaacgct
    11221 atctggcaaa agatgaagag ggtaaggtca ttgaaactcc tcgccagatg tttgagcgaa
    11281 tagcaaaata tatagcatcg gctgactttt tatacggaag agacagcaag catgtttcac
    11341 aggttgctac aaccttttac gatatgatgg ttgaccttct ttttatacca aacagcccaa
    11401 cacttatgaa tgctggcagg cctttaggac aactatcggc atgctttgta ttgcctgttg
    11461 aagattcaat ggatgctata tttgatgcaa taaaatatat ggcattgatt cataaaagcg
    11521 gcgggggcac aggattttca ttttcaaaat tacggccaaa aaatgatgtt gtgcaaacaa
    11581 caaaaggtgt ttcaagtggg cctgtttcat ttatgaacat atttaataca gcaactgaaa
    11641 ccataaagca gggtggtacg cgacggggcg ccaacatggc aatactgcat gttacccatc
    11701 ctgatatact ggagtttata aaagcaaaag aaaagaatga cgcgcttacc aatttcaact
    11761 gttcagttgc cctgagcgat gcctttatga atgcagtgga aaatgatgat gactatgagc
    11821 tcatcaatcc tcgaaataac agggttagcg gtacgcttaa agctcgtgaa gtttttaacc
    11881 tcatcgtccg tatggcatgg aaaaatggtg aaccggggat agtatttata gacaggatta
    11941 atgaaaaaaa tgttttaaaa aaagtgggtc tcattgaaag caccaacccc tgtggggaac
    12001 aaccgcttct tccgtatgaa tcgtgcaatc tgggatcaat taatgtagcg cgatgtgtgg
    12061 taaacaatgg caagtcggta cgtgtggatt acgaactgtt aaacgatatt acctataaag
    12121 cagttcattt tctggataat gtcattgatg ttaacaacta tccgctgcca ataataggag
    12181 aaaaaacaaa agcaaaccgc aagataggac ttggtatcat gggctttgct gatatgctca
    12241 ttgcgttagg cataccctat gattccgaag ctgcgctggg tgttgcaaaa aatattatgt
    12301 cagccatcca ggatgcttca aaagaggctt cgcgtgcact tgctaaagag cggggagcat
    12361 ttccaaactt cccgctttca gagtatgcac acaacggtga tgagccgctg aggaatgcta
    12421 caacaacaac tatcgccccg acaggtacta taagcatcat tgccggtaca tcaagcgggg
    12481 ttgaacccat ctttgcactg gcgtatgtgc gcaatgtcct tgataacagc agattgattg
    12541 aatcaaaccc attatttgtg gaatattgta aagagcgtgg attgtatact gatgagctta
    12601 tgcaaaaggt tgcgcaaacc gggtcagtgg cacatatcga agaactccct gaagatgtac
    12661 gtgctttgtt tgtaacagca catgatattg caccggagtg gcatgttcgt attcaggcag
    12721 cgtttcagga atatgttgat aatgcagttt ccaa
//
LOCUS       DAOSOX010000057        12554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5011_length_12554_cov_6.887111, whole genome shotgun sequence.
ACCESSION   DAOSOX010000057 DAOSOX010000000
VERSION     DAOSOX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_12554_cov_6.887111"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLX22_05815"
     CDS             <1..942
                     /locus_tag="PLX22_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19448.1"
                     /translation="GNPPYINVYCFKGEVNTLFDTGISWTWRLLQKALHEIGLQFKDI
                     HQIIPSHGHVDHYGAANAIVKRSNNNIQVIAHNADAMRIEKGVYANPLVMLRFMQSMG
                     VPKALIVPLAILDVAFKSMGHNVTITHKIIDEVYTLRVGDYNAKLILTPGHSKGQMCL
                     FIEKESLLFSGDHIVPGVKPIILAGFEPNTSFPVSQSHAIFHTALKKITKLHPRVTFP
                     AHGEPFDGKLDRLINTYHKIYQLRKQRIFSILLNNDYTVYSLARKVFKSLKGINLVLD
                     MYLSLSETYTHLEAMCIEGEVIKYSFDNVIYYTAALY"
     gene            1405..2604
                     /locus_tag="PLX22_05820"
     CDS             1405..2604
                     /locus_tag="PLX22_05820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT19449.1"
                     /translation="MRLISIAIPIIFIFVISCFNTVESKNNNTGLTVSFTGDVIMHGP
                     VKSFAFRNNIIDIKTKRTINNGGFDILFEHIKPEFENSDYVVTNMEFPVAPPFTSKPF
                     IFNCTPAVLDAFVSNHINIVTLANNHILDQQYEGLLSTIKILESKNINYIGAGTLINT
                     TKDSINGIVVGDSIKIGIIAYTGVFNYELSRKLKKDIYINDFYSINKVLNDIARIKQK
                     CDFLVMVAHIGEEYKTQPAKKDRDIIHTYCEAGVDCVIGHHPHVIQPVEQFKTNDGRF
                     CTIFYSLGNFIANQSSVHIDAISSVECSTRETIIVQLTVTKNTENNHHYTVIPVMIIN
                     IPDTESKYKYGKRIQPIAIMQYINLLKKENNNSEYINYLINRKNELIKHLFLYDNYQN
                     IKIKDNY"
     gene            complement(2610..3731)
                     /locus_tag="PLX22_05825"
     CDS             complement(2610..3731)
                     /locus_tag="PLX22_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT19450.1"
                     /translation="MSNGISTDNLDALINNCIQTLNINKTKFDNLPDYERALAYYSIY
                     HTNKIHLLLVSNTYKGLATTFTQTGKVFEELAYHYFDTLPTMLTTIHCLELIKSIADD
                     NKKIFLLNHIIDAKKPFAFCLTEENAGSDITAITTVATKNNNQYIINGTKDIVINSGI
                     ASFYIVFARSGKKGRAGLNAFLIPENAKGLCTNKLMLDSFYNSVLGTVTLENVTIDEE
                     YQIGEDGSGYFLLTEMLDKGRPLVAACCVGGSQRAFDIILEHTKKRKQFSKTLYNFQG
                     INFTIAEFETRIHAARLLYQDACKKIDNGEPFSKESSIAKYFASRLFLDITTFGMDVL
                     GYRSIFNASEIKHLNNCAHTMMFIDGTENVQKMIIASLL"
     gene            complement(3724..4068)
                     /locus_tag="PLX22_05830"
     CDS             complement(3724..4068)
                     /locus_tag="PLX22_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT19451.1"
                     /translation="MAFKKIGKKIQIAREEKGITQIELSKMLGITQAALSNYELGKRR
                     LYLQQIEEIAKHLEKPVSYFLDEESSSNIDDELSDITINSITDKIKNLTHYEINELNQ
                     FIDYLLWRRNHE"
     gene            4469..5716
                     /locus_tag="PLX22_05835"
     CDS             4469..5716
                     /locus_tag="PLX22_05835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT19452.1"
                     /translation="MFDYIMNDEQLKLRDEARDFAKWVPREMILDMDAEKIHFPKEFL
                     KEAGKRNLLGIRLPEQYGGRGLNWVDDCIVAEEIGVASYSLACLWGVGADIVCEAIVE
                     FGSEELKQEIVVPLLKGDVYAAECLTEPRGGSDFFGASTVAKKDGSDWIITGQKRFIV
                     GAEGADWFMVYAVTNPEGKAHQRLTCFMVPRTKGVETKYIYGLMGVRGGGAGRLILNE
                     VRVPERYALNGINGGFDVFVRNMLPERLGTAAMTIGSVRPAVEIATRYTSMRKAFGQP
                     IAYFEAVGFKVADSVMLLDATRSLVYSTALAIDSGKVHPGRVRRMVSESKKFVTESAW
                     TIANNCMQVMGGIGYTNVYPLERIVRDIRLSMIWVGTNEIMDLIIQNEWYKEYFKVIS
                     KLNLRDVEQDAPGADQVEEKVYE"
     gene            complement(5741..7342)
                     /locus_tag="PLX22_05840"
     CDS             complement(5741..7342)
                     /locus_tag="PLX22_05840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19453.1"
                     /translation="MKIEFVGGARTVTGSSFIVKNNNFTVMIDCGMFQGTRELRERNY
                     LNLIYAPSQIDSLLLTHAHIDHSGLIPKLVKEGFTQPIYATKATCELCSIMLPDSAHI
                     QEQDAEIINKKRKKLGREYIEPLYTSEDAERCMQYFNPVRYHQTVSIHPDITVSFKDA
                     GHILGSSFIEMNIKDNGKQNAIVFSGDIGQKNQAIIRDPELPDYADILLIESTYGDRL
                     HKSKEDTYNELKKVILESYNKHGNIIIPAFAIERTQELLYTIAQLVKKGEIPPIPVYL
                     DSPLATSATHIFLNNQDCFDDAINNLLRSGDSPLDFPNLHFVKTVEESKWLNKNAKGA
                     IIISASGMCTAGRVKHHLLNNLYKPESAVVFVGYQAEGTLGRRIVDGAHMVKIYGENV
                     AVRAKIYTIGGFSAHADKEGLLDWMSTIKNPKLKVFVVHGEENASALLAEEIRNRFGY
                     TVYVPHWGEIVDLETMKSEYAKYSPFEIADNVESELEHVEKLVQTLKARYKKAKEEGR
                     RINWNQIENDISDIRELVSMINDEL"
     gene            complement(7376..>7975)
                     /locus_tag="PLX22_05845"
     CDS             complement(7376..>7975)
                     /locus_tag="PLX22_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19454.1"
                     /translation="INKGEIVSIVGPSGAGKSTLLNIIGCLDTFQHGYVQVLDQDISP
                     FSYDELATFRNKHIGFIFQMHNLMPEFTALENTMMPLLIRRVSKKEAKHKALELLEHF
                     KIVDRAGHKPAELSGGECQRVAVARALITQPDIILADEPTGNLDSKNSDILIELLFKN
                     CKDKGSTAVVVTHDQSIAAKTQRIITLIDGMIQSETMNL"
     assembly_gap    7976..8075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8081..9385)
                     /locus_tag="PLX22_05850"
     CDS             complement(8081..9385)
                     /locus_tag="PLX22_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19455.1"
                     /translation="MRLYELFIGWRYLKAKKSQGFISFNTFLSLFIVAIGVFVIIVVM
                     SVMTGFQSQIRDKILDVDAHITVSRFFVDNNSEGLPDYKELSNKIKTIKGIRSIEPFV
                     QGQGLVRVKSYIFPVAIRGYGIPGMVPDDIKKFVTAGDKEFSKPTTIAIGEEMAFNYN
                     LRLGDYLEIIVPKGRLTAKEGITPGIGKYRIATYFKTGYYDFDTKLIIMTIPQTQQLY
                     GIGNIVSGLALKLDDIYDMDKIASTVQGLIGYDYQVTTAEQQNQNLFYALRLEKLIMT
                     IILFLVIISASFTIMGTLVMVVMEKRKSIGILKTMGAHPYSIMTIFVLEGFFIGLIGA
                     IVGVITGLVTAINLESIIHWIERTINGVMSWIFITFDLGIFYPVSLVPKNVYYIDSLP
                     TQIQPEFVMIVAILAIFIATVAAILPSWYASRLHPVESIRYE"
     gene            complement(9493..10410)
                     /locus_tag="PLX22_05855"
     CDS             complement(9493..10410)
                     /locus_tag="PLX22_05855"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HOT19456.1"
                     /translation="MKQFCHYGIAIMLLFCLYSIAFANEDHDFYITTALGCGFSQGVL
                     GEYVYDNGRKVSQLHWNIDHLYYYFYEVALYYGWFTTGLSLQNSYDHSIGTINDSDWT
                     DNNNPYVKTNFSHHDNYLEYMHNYNVYCGFLVVNNKDFQLIMKAGYIYKNIKMSARDG
                     YVEYPPGSPKVSMYGIGIIYQQEYSIPYIAIEAMYTMYNLSLSGGLSCGIFADADDVD
                     NHVKRDIDFHDDFDGILYLCGKAGIQYSFTHFIIGGKVIYEYVPVTKGDEYYINNSNG
                     SKSPVYNNCGGCKLAVMSIELFISIKVEL"
     gene            complement(10474..11133)
                     /locus_tag="PLX22_05860"
     CDS             complement(10474..11133)
                     /locus_tag="PLX22_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19457.1"
                     /translation="MMRKGIVSFVCIMIVAMSNIFYTTYLFAVDIALNYSPCSLLADY
                     KENSRTINIKDDSVDYFDKWGYLSKASLLFLFPNNDKEHPYMTGYFIDILHKSVSIEP
                     VDIKYFEKGGDIHIVSGIAGIMFRGPVKTTNIYFDFGFGIGYTAIFKDSYGAHSYGND
                     TDYTFKLGAGYKVNLSHSIACNFGVDWIAPGLSSYHKLNHGGYVNLYYGGIYPYIGVA
                     F"
     gene            complement(11307..12200)
                     /locus_tag="PLX22_05865"
     CDS             complement(11307..12200)
                     /locus_tag="PLX22_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19458.1"
                     /translation="MNESDRELIDGQLTIIEDELSAFMTRNFRAECYDLSSKSAMAAE
                     IMVNGNISQAMYTLAEKDVLIQFSFHEMLYDNIKLNNGQDKTIKSNKYIVYEFKNNST
                     NQERAWENKEISVREVSNVNMTDTNTTDTDIENNQIELSLPMEDSNLAVEFETELYQN
                     LETKQGVEVNGNEDTNVISLDNGVEITVEYETISNTEKGLVKKIKKAAHKVKHSVSNA
                     WDKYGPQIYGTLCIISGVVILAEYYGAAFMAGITSGFAAIPLIYGAHPFATAIGTGLI
                     TSGCVSIIKNKYYIGFSYSYK"
BASE COUNT         4114 a         2128 c         1996 g         4216 t
ORIGIN      
        1 ggtaatccac cttacattaa tgtttattgt tttaaaggcg aagtaaacac cctttttgat
       61 accggaatat catggacatg gagattattg caaaaagctt tgcatgagat tggattgcag
      121 tttaaggata ttcatcagat tataccatca catggtcatg tggatcatta tggtgcagca
      181 aatgcaatag tgaaaagaag caataataat attcaggtga tagcccataa tgctgacgca
      241 atgcggatag aaaaaggagt ttatgccaat ccattggtaa tgttgcgatt tatgcaatct
      301 atgggtgtac caaaagcgtt gattgtgcca ttagcaatcc ttgatgttgc atttaaatct
      361 atgggtcata acgttaccat cacccataaa ataattgatg aagtttatac attacgagta
      421 ggagattata atgcaaaatt gattttaaca ccaggacatt caaaagggca gatgtgttta
      481 tttattgaaa aggagagttt gctattttct ggagatcata ttgttcctgg agtaaaacct
      541 attattttag cgggctttga accaaataca tcattccccg tttcacagag tcatgctatt
      601 tttcatactg cattgaaaaa aattacaaag ctacatcccc gtgtaacatt tcctgcacac
      661 ggtgaaccct ttgatggaaa acttgacagg ttaatcaaca cctatcacaa gatataccaa
      721 ttacggaagc aacgaatatt ttccatacta ttgaataatg attacacagt ctattcgctt
      781 gcacgaaagg ttttcaaaag tcttaaggga ataaatttag tgctcgatat gtatctttca
      841 ctttcagaaa catacactca tttagaggca atgtgcattg aaggtgaggt catcaaatat
      901 tcatttgaca atgtaatcta ttatactgca gctttatatt aattaaggtt aaactactat
      961 actatctggt tccatgtttc atggtatatc atgttcattt tttatgcgat agttactgct
     1021 tatgaatatt ttcgtaaata acgtattaac agcagcatta caatcaatgc caaaaaattt
     1081 atttctgttg atacctatga cacttaaatt atacggagag caggttcacg gtatgctgtc
     1141 ggtttgttac atttggtggt gatagttact attatagagt aattggaatg tgtttaatgt
     1201 ttttatgaat acaatttcta atgtgacata atatatctta tcagaagtaa tgaaagttca
     1261 aatggaagtt cagtatctat ttttattaga ttacgataaa tgaatagaca aatttaaacc
     1321 tgtataaaat atcttttaaa agatattgca taatggatat gtactttata atgagccaat
     1381 aaaccatagg gactatcata tagtatgaga ttaataagca ttgcaatccc aataatattt
     1441 atatttgtta tttcctgttt taatacggta gaatctaaaa acaataacac cgggttaaca
     1501 gtatcattta caggagatgt tattatgcat ggtcctgtaa agtcgtttgc tttcagaaat
     1561 aatattattg atataaaaac aaaacgaact atcaataatg gtggttttga tatactattt
     1621 gaacatataa aaccagaatt tgaaaattca gattatgttg ttacaaatat ggagtttccc
     1681 gttgctccac cgtttacaag caaacctttt atattcaatt gcactccagc agttttagat
     1741 gcatttgtga gcaatcatat aaatattgtg acacttgcaa ataaccatat tcttgatcaa
     1801 caatacgaag gtttactgtc caccataaaa atacttgaat caaaaaatat taattatatt
     1861 ggtgcaggta cactgataaa cacaacaaaa gattcaataa atggcattgt agttggtgat
     1921 agtatcaaaa taggcattat tgcctataca ggagttttca actatgaact atcacgaaaa
     1981 ctaaaaaaag atatttatat aaatgatttt tatagcataa acaaagtatt gaatgatatt
     2041 gctcgtatta aacaaaaatg tgattttctt gtaatggtgg ctcatattgg tgaagaatat
     2101 aaaacacaac ctgcaaaaaa agatagggat attatacaca cctattgtga ggcaggcgta
     2161 gattgtgtta taggacacca tccgcatgtg attcaacctg ttgaacagtt taaaacaaat
     2221 gatggacgtt tttgtaccat attttacagc cttggtaatt ttatagccaa ccagtcttca
     2281 gtccatattg atgccataag cagtgttgaa tgcagtacac gtgaaacaat tattgtacag
     2341 cttactgtaa caaagaatac tgaaaacaat caccactata cagtaattcc cgtaatgata
     2401 atcaacattc ctgacactga atcaaaatat aaatatggga agagaattca acccatcgct
     2461 attatgcagt atataaacct gcttaaaaaa gaaaacaaca acagtgaata tataaactat
     2521 ttaataaaca gaaagaatga gttgataaaa catctgtttt tatatgacaa ctatcaaaac
     2581 ataaaaatta aggataacta ttaatacatt tatagtagcg aggcaatgat cattttctgg
     2641 acattttccg tgccatcaat aaacatcatg gtatgcgcac agttgttaag atgttttatc
     2701 tcgcttgcat tgaaaatgga acggtagcct aaaacatcca taccaaaagt tgtaatatct
     2761 aaaaataatc gtgatgcaaa atattttgct atagaggatt ctttgctaaa aggttcacca
     2821 ttatcaattt ttttacatgc atcctgatat aataatcgtg ctgcatggat acgggtttca
     2881 aattcggcaa ttgtaaaatt tattccctga aaattgtaaa gagtttttga aaattgtttt
     2941 cgtttctttg tatgttctaa tattatatca aaggctcttt gtgatccacc aacacaacaa
     3001 gcagctacca atggccttcc cttatcaagc atttcagtta acaaaaaata accacttcca
     3061 tcttctccta tttgatactc ttcatctata gttacgtttt ccagggttac cgttccaagc
     3121 acactattat aaaaactatc taacattaat ttgtttgtgc ataatccttt agcattttcc
     3181 ggaattaaaa atgcatttaa acctgccctg ccctttttac cgctccgtgc aaaaacaata
     3241 taaaacgatg caatgccgct gtttatgaca atgtcctttg taccattaat aatatattgg
     3301 ttattatttt ttgttgcaac tgttgttata gctgtaatat ccgagccagc attttcttcg
     3361 gttaaacaaa atgcaaaagg cttttttgca tcgatgatat gattaagtaa aaatattttc
     3421 ttattatcat ccgcaataga ttttattaat tccagacaat gaatcgtagt taacatggtt
     3481 ggcaaagtat caaaataatg atacgcaagt tcttcaaata cttttcccgt ttgagtaaat
     3541 gttgtagcaa gacctttgta tgtattactt accagcagca gatgtatttt attagtatgg
     3601 tatattgaat aatatgccaa agccctttca taatcgggta aattatcaaa ttttgtttta
     3661 ttaatattta atgtttgtat acaattattt attaatgcat caagattatc agttgatatc
     3721 ccgttactca tgatttcttc tccatagcaa ataatcaata aattgattta gttcatttat
     3781 ttcataatgt gtaagatttt tgattttatc agtgatagag ttaatggtaa tatcagatag
     3841 ttcatcatca atgtttgaag aagattcttc atcaaggaaa tacgatacag gtttttcaag
     3901 atgttttgca atctcttcta tttgctgtaa atacaatctg cgtttgccta attcatagtt
     3961 tgataatgca gcctgtgtaa tgccaagcat ctttgacaat tcaatctgcg ttatgccctt
     4021 ctcttcacga gctatttgaa tctttttgcc aatcttttta aatgccataa catatactca
     4081 ttttttatag taccatcgat gtcatagaaa tagtgaaaaa tataagtttt tatacttctc
     4141 tgaaaacata ccatgatagt ctcgcatctt acatttagca gacaggtagt atactgtgtt
     4201 acgcgattat taatgcatat ttactttttt atgacaataa aaaatttttt ttgatatttg
     4261 tattgacagt ataacagcta taacactaat ataacaatat gttatatttc ataggtaact
     4321 atcgtacaaa aaattgttga atatagtttt gagtaatagc atcataatta tcagcatata
     4381 ttgttatcga tagttgttat cgcaataatg aactgaacct gataaatgaa tgtagcaatg
     4441 aataccatat atatgtgagg aggcatatat gtttgattat atcatgaacg atgaacaatt
     4501 gaagttgagg gatgaagctc gtgattttgc aaaatgggta cctcgtgaaa tgattttaga
     4561 catggatgca gaaaaaatac atttcccaaa agaattttta aaagaggctg gaaaaaggaa
     4621 tttgctgggc atacgattgc cagaacaata tggcggcaga ggactaaatt gggtagatga
     4681 ttgtattgtt gcagaagaga ttggcgttgc cagttattca ttagcttgtt tatggggcgt
     4741 tggtgctgat atagtgtgtg aagcaatagt tgaatttgga agcgaagaat taaaacagga
     4801 aatcgttgta ccgttattaa aaggtgatgt ttatgctgca gaatgcctga cagaaccacg
     4861 cggcggttca gatttttttg gagcatcaac agttgccaag aaagatggca gtgattggat
     4921 tattaccggt caaaaacgat ttattgttgg agctgaaggt gctgattggt ttatggtata
     4981 cgcggtaacc aatcctgaag ggaaggctca tcagcgttta acctgcttta tggtgccacg
     5041 aacaaaaggt gttgaaacaa aatatatcta cggacttatg ggagttcgtg ggggtggtgc
     5101 tggtcgctta attttaaacg aggtacgagt ccctgaacgg tatgctttga atggtattaa
     5161 tggtggtttt gatgtttttg tcagaaatat gttgcccgaa cgtttaggta ccgctgcaat
     5221 gactattggc agtgtgcgcc ctgcagttga gatagcaacc cgatatacct ctatgcgtaa
     5281 agcatttggt caacccatag catattttga agcagttggc tttaaagtgg cagatagcgt
     5341 catgttactg gatgctaccc gatcactggt atacagtaca gcgctggcta ttgattcagg
     5401 caaagtacat ccaggaagag tgcgaaggat ggtgtccgaa tccaaaaagt ttgtaaccga
     5461 atccgcatgg actatcgcca ataactgcat gcaggtaatg ggtggtattg gctataccaa
     5521 tgtttatcca cttgaacgta ttgtaaggga tatcaggtta tccatgatat gggttggtac
     5581 caatgagatt atggatttaa tcattcagaa tgaatggtat aaagaatact ttaaggtaat
     5641 ctcaaaatta aatttgcgag atgttgaaca ggatgctcct ggtgcagatc aggttgaaga
     5701 aaaggtttat gagtgatagt tattaaagat tgggcatgcg ctacagttca tcgttaatca
     5761 ttgatacaag ctcgcgtata tcagatatgt cattttcaat ttgattccag ttgatgcgcc
     5821 tgccctcttc ttttgctttt ttatatcgtg cttttaaagt ttgcaccaat ttttcaacat
     5881 gctcaagctc agattcaaca ttatcagcaa tttcaaatgg actatatttt gcatattctg
     5941 atttcattgt ttcaagatca actatctcac cccaatgagg cacataaact gtatagccaa
     6001 atctgttacg tatttcttcc gctaacaatg ctgatgcatt ttcttcgcca tggacaacaa
     6061 agacttttaa tttaggattt ttgatagtac tcatccaatc aagcagtcct tctttatcag
     6121 catgagcaga aaatccacca atagtataaa ttttggctct aacagcaaca ttttcgccat
     6181 atattttgac catgtgtgca ccgtcaacaa ttcgtcttcc cagagtacct tctgcttgat
     6241 atccaacaaa tacaaccgct gactcgggtt tatataaatt attcaacaga tgatgcttta
     6301 ctctccctgc agtacacatc ccacttgcag atatgattat ggcacctttt gcattcttgt
     6361 ttaaccattt tgactcttcc actgttttaa caaaatgaag attaggaaaa tccagaggac
     6421 tatcgcccga acgcaataag ttatttatag catcatcaaa acaatcctgg ttattcaaaa
     6481 aaatatgagt agctgatgtt gctaaaggtg aatctaaata aacaggtatt ggggggattt
     6541 cccccttttt cacaagctgt gctatggtgt ataataattc ctgggtacgt tcaatagcaa
     6601 atgctggaat aattatatta ccatgtttat tatatgattc aagtataact ttttttaatt
     6661 cattataggt atcttctttg cttttatgaa gtctgtcacc ataggttgat tctataagca
     6721 atatatcagc atagtcggga agttcaggat cacgaattat tgcctgattt ttttggccaa
     6781 tatcaccaga aaatactata gcattttgtt tgccattatc tttgatattc atctctataa
     6841 acgatgaacc caatatatga ccagcatcct taaaacttac tgtaatatca ggatgaattg
     6901 ataccgtttg atgatatctc acaggattaa agtattgcat acaacgctct gcatcttcag
     6961 aggtgtatag tggttcaata tattcgcgtc ccaatttttt tcgcttttta ttaatgattt
     7021 cagcatcctg ctcctgaata tgggcactat cgggcaacat aatactacaa agttcacagg
     7081 ttgcctttgt ggcatatatt ggctgagtaa aaccttcttt tactaatttt ggaataagtc
     7141 cactgtggtc aatatgtgca tgggttaaaa gtaaactgtc tatctgtgaa ggtgcataga
     7201 tgagattaag ataatttcgt tcccttagct cacgtgttcc ctgaaacata ccgcaatcaa
     7261 tcataacggt aaaattatta ttcttaacaa taaatgatga accagttacc gtgcgtgctc
     7321 caccaacaaa ttcaattttc ataacaccat aattcctttt tcattttatt attaattata
     7381 aattcattgt ttcactctgt atcatcccat ctattaatgt aatgattctt tgtgtttttg
     7441 ctgctatgct ctgatcgtga gtaacaacca ctgctgttga gcctttatcc ttacaatttt
     7501 taaataaaag ttcaattaaa atatccgagt ttttactgtc cagattacct gttggctcat
     7561 cagccagtat aatatcaggt tgcgttatga gtgctcgtgc aacagcaaca cgctgacatt
     7621 caccacccga caactcagca ggtttatgcc ctgctctatc aactattttg aagtgctcta
     7681 acaactcaag tgctttgtgt tttgcttcct tttttgatac tcttcgtatt aaaagaggca
     7741 tcatggtatt ttccagtgcg gtaaattcgg gcatcaggtt gtgcatctgg aagataaaac
     7801 caatatgctt atttctaaat gttgccagtt catcatacga aaatggtgaa atatcctgat
     7861 ctaatacctg cacatatcca tgttgaaagg tatcaagaca gccaataata tttaacaatg
     7921 ttgattttcc tgcacctgat ggaccaacta tcgaaactat ctcaccttta ttaatnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaataa ttattcatag cgtattgatt
     8101 caacaggatg caatcgtgat gcataccatg atggcagaat ggcagccaca gtggcaataa
     8161 agattgccag tatagcaaca atcataacaa attcaggttg tatttgcgtg ggaagcgaat
     8221 caatataata tacattcttt ggcaccagcg aaacaggata aaaaatacct aaatcaaaag
     8281 ttataaatat ccatgacatt acaccattaa tagttctctc aatccagtgt atgattgatt
     8341 ccaaattgat ggcggtaaca agcccggtaa taacgccaac aattgcacca atgagcccga
     8401 tgaaaaaacc ttcaagcaca aagatggtca tgatggaata tggatgcgcc cccattgttt
     8461 ttaatatgcc aatagattta cgtttttcca ttaccaccat gacaagtgta cccattatgg
     8521 taaatgaagc tgagattatc actaaaaata aaataatagt cataattaac ttttcaagac
     8581 gcagtgcata gaataaattt tgattttgtt gttcagcagt tgttacctga taatcatagc
     8641 caatgagtcc ctgaacagtt gaagctattt tatccatatc atatatatca tcaagtttca
     8701 aagcaagtcc gcttacaata ttaccaatgc catagagctg ttgcgtttgc ggtattgtca
     8761 tgatgataag cttggtatca aaatcgtaat atcccgtttt gaaataagtt gcgatgcggt
     8821 atttcccgat gccgggcgtt atcccttctt ttgcggttaa ccttcctttg ggaacaataa
     8881 tctctaaata atcgcctaag cgcaaattat aattaaatgc catctcctcg ccaatagcta
     8941 ttgtagtagg tttactaaat tctttatcac cggcagtgac aaattttttt atatcatcag
     9001 ggaccatacc gggaatgcca taaccacgga ttgctacagg aaaaatatat gacttaacac
     9061 gcactaaacc ctgtccctgg acaaatggtt ctatagagcg tattcctttt attgttttta
     9121 tcttgttcga tagttcttta taatcgggta aaccttcgct attattatct acaaaaaagc
     9181 gactcactgt tatatgcgca tcaacatcca gaattttatc gcgaatctga ctctggaagc
     9241 ccgtcattac tgacataacc acgataatga caaatacgcc aatagcaact ataaacagcg
     9301 ataaaaatgt attaaaagag ataaatccct gagatttttt tgccttaaga taccgccatc
     9361 ctatgaacag ttcatataat cgcatagata ttccttgaaa aaaattataa cgaatgtatt
     9421 aaattatagt attttgtcaa atgtatttat ttatatgaag atttcagaaa atgtttatat
     9481 aaaatgcagc atttataatt ctacttttat tgaaataaaa agttcaatgc tcattaccgc
     9541 aagtttacag cctccacaat tattatatac tggagatttg cttccattgc tattattgat
     9601 ataatattca tcgcctttgg ttaccggtac atattcatat atcactttgc ctccaatgat
     9661 aaaatgggta aatgaatact gtatgccagc ttttccacaa agatagagta tcccatcaaa
     9721 atcatcatgg aaatcaatgt cgcgtttaac atgattgtca acatcatcag catcggcaaa
     9781 tataccgcag gatagtcctc cagatagtga taaattatac atagtataca tagcttctat
     9841 tgcgatatag ggaatggaat actcctgttg atagataata cctattccat acattgatac
     9901 ctttggcgaa ccgggtggat attctacata gccgtcacgt gccgacattt ttatattttt
     9961 atagatgtat ccagctttca ttataagttg aaaatcttta ttattaacta ctaaaaatcc
    10021 acagtaaaca ttataattat gcatatattc cagataattg tcatgatgcg aaaagttagt
    10081 ttttacatat ggattattat tatcagtcca atccgaatca tttattgttc caatagaatg
    10141 gtcataacta ttttgcaatg ataatccagt ggtaaaccag ccatagtata atgctacctc
    10201 ataaaaatag tagtaaaggt gatcgatatt ccaatgtaat tgacttactt ttctaccatt
    10261 atcatacaca tattcaccaa ggacaccttg tgaaaagcca catcctaatg ccgttgttat
    10321 atagaaatca tggtcttcat tagcaaaagc tatagaataa agacaaaata ataacattat
    10381 agctatgcca taatggcaga attgcttcat cactttattc accatgcaaa tttttacaaa
    10441 tcaaaatttg cgcatatact ataatagttt acattaaaat gcaacaccaa tataaggata
    10501 aattcctcca tagtataaat tcacataacc gccatgattt aatttatggt agctacttaa
    10561 tccaggtgct atccaatcaa caccaaaatt gcatgctata gaatgtgaaa ggttaacctt
    10621 atagcctgcc cctaatttga atgtgtagtc tgtatcattg ccataagaat gcgcaccgta
    10681 actatcttta aaaatagcag tatagcctat tccaaaacca aaatcaaaat aaatatttgt
    10741 tgttttaact ggacctcgga acataattcc agcaataccc gatacaatat ggatatcacc
    10801 acctttttca aaatatttta tatcaaccgg ttcaatggaa actgatttat gcaatatatc
    10861 aataaaatat cctgtcatat agggatgctc tttatcattg ttgggaaata aaaacaatag
    10921 tgatgcttta ctcaagtaac cccatttatc aaaataatct acactgtcat ctttaatgtt
    10981 tatagttcgc gaattttcct tataatcagc taaaagagag catgggctat aatttaatgc
    11041 aatatcaaca gcaaaaagat aagtagtata aaaaatgttt gacatagcta ctatcattat
    11101 acaaacaaat gatacaatac ctttcctcat catagtacac cccttttatt atcctttaca
    11161 ttatataatc ttactcaaaa tcattatata accttatata agagcaccaa acaaatagca
    11221 aggtgccctg atatctactt acactaaaac gattatttta ttattgtaaa aaattaaata
    11281 tttagctcat ctaatatgta ctattactat ttatagctat aactaaatcc tatatagtat
    11341 ttattcttta taatagatac acagcctgaa gtaatcaaac ctgtaccaat tgcagttgca
    11401 aaaggatgag ctccgtatat taaaggaatt gcggcaaaac cactagtaat accagccatg
    11461 aaagctgcac cataatattc agctaatata acaacacccg atataataca taatgttcca
    11521 tatatctggg gcccatattt gtcccatgca tttgatactg aatgcttaac cttgtgtgct
    11581 gcttttttga tttttttaac taatcctttc tctgtatttg atattgtttc atattctaca
    11641 gttatttcca cgccattgtc caatgatatt acattagtat cttcatttcc attaacttct
    11701 acaccttgct ttgtttctaa attttggtat aattctgttt caaattcaac tgctaaattt
    11761 gaatcttcca ttggtaatga taattctatt tgattatttt caatatctgt atctgttgta
    11821 ttggtatctg tcatattaac atttgatact tctctaacag atatttcttt attttcccaa
    11881 gccctttctt gatttgtgct attattttta aattcatata ctatgtattt attactcttt
    11941 attgttttat cttgtccatt atttaacttt atattatcat acaacatctc atgaaatgaa
    12001 aactgaatca aaacatcttt ttctgctaat gtatacattg cttgactaat atttccattt
    12061 accataatct cggctgccat agcagatttt gaagataagt cataacattc tgccctaaaa
    12121 tttcttgtca taaatgctga taattcatct tcaattattg ttaattggcc atcaataagt
    12181 tctcggtcag attcgttcat agatcgcatg gtatcttctg taacaattgc tttaaaataa
    12241 taatcatatt tctttgcaat gtcattggat gtaggagtat aattgctaca cgaaacaata
    12301 taagtaaacg atattatagt aataaataaa catgtttgta acaataattt ttcagttatg
    12361 agggaatgtt ttatttgaga tttgagattt gagatttgta atacatattt aatcctcgtg
    12421 tttatataat atattttttc tttaagttat aatagattat ttgtcaagta tatttttatt
    12481 atttaataga aaatatatag taactactca gaaactgtag gaaaaatcaa gcaggaaatg
    12541 atagaggagt aaga
//
LOCUS       DAOSOX010000058        12523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5022_length_12523_cov_8.512432, whole genome shotgun sequence.
ACCESSION   DAOSOX010000058 DAOSOX010000000
VERSION     DAOSOX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5022_length_12523_cov_8.512432"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..567)
                     /locus_tag="PLX22_05870"
     CDS             complement(163..567)
                     /locus_tag="PLX22_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOT19459.1"
                     /translation="MKIVLDTNVILSAFITQGLSSRVIDICIDKHQLFISQWIVNEVI
                     EKLNTKFKTPKKELKRVKDFLLTAFRFIQPEGEMPDLCKDKDDNNILYLAQYIDADLI
                     ITGDKDLLELKIIKKSRIINPRTFMEKYHNMH"
     gene            complement(564..782)
                     /locus_tag="PLX22_05875"
     CDS             complement(564..782)
                     /locus_tag="PLX22_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT19460.1"
                     /translation="MREILTISIDSKLKEKVEKAAEKFNVSKSELVKKAIEKYILHEE
                     FQEIRSMLIPFGEKAGYFTDEDIFKDFS"
     gene            complement(1022..2152)
                     /locus_tag="PLX22_05880"
     CDS             complement(1022..2152)
                     /locus_tag="PLX22_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT19461.1"
                     /translation="MAESILHFFMFYGAAFSVIIAIGQLLQKKSETINRIYAFSFLGL
                     GIWLLQISIFSSGLFDGTAVGYYVCIFSIPIVFTVPPLMVIRYTWIMSSLFNFQKKYY
                     LLFSPVVVSLLYIMIQFFIDSDCGYTCFPALPILSHTFNELSLYDTILYLFFIFPNIY
                     LISFMSQNLFMTSYIWKKSNWNKVPGVSRWGYIFAMSIVISNIVYVIGVFISLRVVEW
                     AVILANTAMTFVYLVTQRLPDYNKLLKNEALKARYERSRIKGLDVDSVIQRLYEIMVD
                     EKAFADEELSLPDLARDLGITVHQLSEILNEHIKKNFNTFVNEFRVKEAQKLLVDEPD
                     RSIISIGFAVGFNSNTTFCTVFSKMTGVSPNQFRKDNIKKDE"
     gene            2319..4160
                     /locus_tag="PLX22_05885"
     CDS             2319..4160
                     /locus_tag="PLX22_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT19462.1"
                     /translation="MAQHENVNYEKEFEEYLKRQKHLGLMALDRAEKFGDSKIAVRHK
                     AYGTWEAFTWKQFGDLIKAAAKGLLSLGVKEFEFVGIFSNNRVEWAVVDYACFTIRAC
                     SVPVYATNSAKELEYIVNDCGIRILFVEKQEHYDKAMSVFDNCKTLEKIIVFDRNTNI
                     VNSDKVMLFDDFIQLGKNSGLDAQLQERLSKVQSDDLSTLIYTSGTTGEPKGVMLTHK
                     NWFAMLFGTGYHITVEPTDVNLAFLPLSHVFERAWSYFILCNNAQVDYCHDTKALLDF
                     LKESRPHFMCSVPRMWEKIHATIMDGIKNSPPAKQKIFNWALEVGGNYQYLKKDNKSI
                     PLGLRLKHAIATKLVLHKIRDIFGGRNKVFNCGGSAFSGEISEFFFKAGVFILQGYGL
                     TECFVICVSNPHRNKFGTCGPVVPLMQVRISDEGEIQAKGPSMTQGYWNKPELTKALF
                     TPDGWVKTGDVGFIDSDGFITVTDRLKDLFKTSGGKYIAPQQIETLLNEDFYFEQVAA
                     IGDGRKFVSALIVPAFEPLKNWAKSNNIPFTSHEDLIKHPAVIQFYRERIDELTKNLG
                     QVEKIKKFTLLPNEFTQEKGEITPTQKIRRKVINEHYKDIIEQMYKD"
     gene            4312..5184
                     /locus_tag="PLX22_05890"
     CDS             4312..5184
                     /locus_tag="PLX22_05890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19463.1"
                     /translation="MHINIYTLDEITKTYENIDDHLSTRQIIRKFSQNKNDIRLLSLE
                     LCNLSHYNNILDLGCGYGFFTEALNNRIASGACITGVDVIGGENKRMFLSTCVQSGYN
                     AQFIEDSAHIICNIPDKTYDCIIASYSLYFFPDLINHIARILTNDGTFIALTHSELTL
                     SQITSIFIESIHHVIPLSDSSLHIAKLFKSFSAENGFVQLQPYFDYIMYLRYPNNLIF
                     KSSDIDHCISYIHKKQYLFFKDIALLDRDEYDALINDFYNRLRWHVSSYKDFIVNKDD
                     AIFIARNPVKHINE"
     gene            5292..6293
                     /locus_tag="PLX22_05895"
     CDS             5292..6293
                     /locus_tag="PLX22_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT19464.1"
                     /translation="MKKKRIYCSFCGAPITARFIDEKYRDHCDNCNTTFYENPLPVAS
                     CIVINKNREVLLVLRKNEPYKDMWCLPIGFAESGESIEQAALRELQEEAGVNGEIVRI
                     IDVDTVSNYFYGDLAIITFEVQQLSETYKPGDDAADAKFFSLDNYPQLAWESNEKALH
                     KFIDLYKDIWTMIDSLKTIQPDITTHHDIPREKSKQYTFIANIIASMIDDEIELFTQQ
                     WKREMPNVNNNIFHFLIFIHQMALHTIKLWLTGNRIWKNFREFYIMGKQLKKENISLK
                     DILSAIALSRKCIWIQAIEKNILHSPLEVYTMLEINNRIILFYDKISYFLIKGYEHS"
     gene            complement(6341..8419)
                     /gene="fusA"
                     /locus_tag="PLX22_05900"
     CDS             complement(6341..8419)
                     /gene="fusA"
                     /locus_tag="PLX22_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT19465.1"
                     /translation="MPLRNIRNIGIAAHIDAGKTTVTERILYFTGVTRKLGEVHDGQA
                     VMDFMKQEQERGITISSASISCQWNDHNINIIDTPGHVDFTVEVERSLRVIDGLIAVF
                     CAVGGVEPQSETVWNQADRYKVPRIAFINKMDRVGADFYEVVSQMDRYLDANPVPFQI
                     PIGVEDSFEGCIDLVEQRAIVFRENERLIVDIPDDYKGKAKEARKHIIEKLADFDDEI
                     MELYLAEKEMPSELIKKTAREATLKMLITPVFCGAAYKNKGIQLLLDAVVDYLPSPVD
                     SGAIVGIDVDYPEKTHTRHPSTHEPFAALAFKLINDLYVGQQTFIRIYSGKLQSGMQV
                     YNSTKGKYERIGRIMRIHAKEREEISEAGAGEIVALIGLKFTKTGDTLCDTNNPLLLE
                     SIYVPPSVIDLQVMPASKKEEEKLGIALHKLATEDPSFNVRVDEETGETIISGMGELH
                     LEIMVDRLKEEFGLNVQVGEPSVAFRETITAEAEQETKFVKQTGGKGQYAHCVMRIEP
                     NPGKGYEFIDHIKGGAIPNEFIPSIQKGIHAAMEEGILADFPVVDVRVVLLDGSFHPV
                     DSSDMAFRTCASICFKEAFMKAKPILLEPQMKIEVNTPDEYIGDVIGDLNRRRGKVES
                     MRRFRKGAQKVNGFVPLMEMFGYATTLRSLSSGRANYAMEFDKYVALPKEMQEKVLAK
                     IKEKKRRQSA"
     gene            8539..8706
                     /locus_tag="PLX22_05905"
     CDS             8539..8706
                     /locus_tag="PLX22_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19466.1"
                     /translation="MVGHGYKDSKHVNILKIFFIFFNKTYHFYKITNLYGILTTILVA
                     IVTLNEPFLMQ"
     gene            8976..10427
                     /locus_tag="PLX22_05910"
     CDS             8976..10427
                     /locus_tag="PLX22_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19467.1"
                     /translation="MDNNDRSLSIFGNPIPKHTYNKAMKQKARFAKKFGFNPNDRYEL
                     SALPNPILKKYCNLYTVSQEEGSEIAKSKSIIIGTIRMGYGHYRIAMAIASAAHSMGI
                     VPYWFDLLSFDTAGAKIIKHMDNLYSFGSRLSQQFYLFNKLYWEHLTAIGFKKLTYNA
                     TDQAMTQLFANIYQNLPQSVPFVATHAWASQAAVHAGTKKVINMIPDNWPLALHLSEG
                     AIHTVQTPSAYFGYRTLKNMGKKNQILNPMPKDDIYYTGHYVDHELVANVEKDCNARL
                     TRLKNKKPRRFLLSIGGAGAQQKLCMDIIMHCIPLLENEKLTLIINTGDHKAIFDMIL
                     NTGFSPRVPKTSYTQWAETEKLVNTLTTRDVPGLHIVYNEDIFSAVYATNILMRVCDC
                     LITKPSELAFYPVPKLFVAHVGGHEMWGAIRGAEIGDSTVECETVQHTLQAMDLLIYD
                     DDLLTLYCNNIIKAKSIGIYNGAYEVIKLAFGK"
     gene            complement(10492..11286)
                     /locus_tag="PLX22_05915"
     CDS             complement(10492..11286)
                     /locus_tag="PLX22_05915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT19468.1"
                     /translation="MGNRSDKPMNPLELLAYVLAKEIKDHEVVYIGTGLPMVAGILAR
                     KTHAPNIVLVYESGAQDPMEGGMPWSVGGPATWRKSPLILEMSYSFGQIYNGYVDKAF
                     LGFAQIDMYGNVNTHMIGKDFLKPDHRLTGSGGNNDLSSLAENMILVGLQSPQKFVPR
                     VDFITSPGFIDGGDSRQKAGLLGKGPVTVITQAAVFDFEPETKRMRVKSLHPGVSFDL
                     CQLACGFELVKPAGDIPVTEVPSKEILEIIRNDVDPRGVFTSIPGM"
     gene            complement(11335..12444)
                     /locus_tag="PLX22_05920"
     CDS             complement(11335..12444)
                     /locus_tag="PLX22_05920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT19469.1"
                     /translation="MGKFSFNEEAVKNLKITDTSVLERLIDFEHARKWHLSKDRSRKD
                     KRMSLKEAVAEYVHDNDIMTDGGFCYVRTSIQSYFEVIRQQKKNLQFCSSPNTNHSYM
                     INAGCARYSHNSYLGAEMRGIDRNYTRQLKAGKVTILSEWSHGTMAQGFKAAQLGLPG
                     VFSKQLLGSDMIKYNPYVKVMQNPMRSDPDPVAYIPAIYPDVSIIHVHAADKKGNARF
                     FGPAINDIAMAAATRKLIITAEEIVPESDIRNNNKGVSIPAMYVDAVVELPYGAVPGQ
                     MPGCYYWSRQWWEMAFRFGMKSDENFQKFLDYWVFGVKDQYEFIEKLGGSKWIAESRR
                     LTKAEEYDNEDEGVDFNYEEFQGDWPEDITKMKFY"
BASE COUNT         4089 a         2446 c         2045 g         3943 t
ORIGIN      
        1 ctcttatttt tctattgcaa gcattttttt aaagatttgt tcagaggtct caattgtaaa
       61 cagcaggtat aacatcaatt cttctgtttg aaatgaaaat catttatgat tcccctgcat
      121 taaaaagctg atgtcaagca atttgagcgc ccatcatcat aattaatgca tgttatgata
      181 cttttccata aatgtccgag gattaattat cctggacttt ttaatgattt ttaattccaa
      241 caaatcttta tcacctgtta taattaaatc agcatcaata tattgagcaa gatataaaat
      301 attgttatca tctttatctt tacataaatc tggcatttca ccttcaggtt gaataaaacg
      361 aaatgcagtt aataaaaaat cctttactcg cttaagttct tttttaggag ttttaaattt
      421 cgtatttaat ttttcaatta cttcattaac tatccattgg gagatgaaaa gttgatgctt
      481 atcaatacaa atatctataa ctcgagaaga taatccttga gtaataaaag cagataaaat
      541 gacattagta tctaatacaa ttttcatgaa aaatctttaa atatgtcttc atcagtaaaa
      601 taacctgcct tttcgccaaa gggtatcaac attgatctaa tttcttgaaa ttcctcatgt
      661 aagatatact tttcaattgc ttttttgact aattcactct ttgaaacatt aaatttttca
      721 gcagcttttt cgactttttc ttttaatttt gagtcaatac ttattgtcaa tatttctctc
      781 atatacttca ccagtttaaa taatttatag ttcagtgtaa taaataatat tacagaagtc
      841 aataaatatt ttgtttttga agacattata gctggtttca ccattttact gtgtggcatt
      901 ggcattcagt ttttaggatt ataaaagttg taatacatcc cggttataat cttagaaaat
      961 ttttttccaa acagcataaa acatcttgtc aggaaacaaa tgaacatgaa aaggtatact
     1021 attattcatc tttttttata ttatcctttc tgaattgatt tggcgaaaca cctgtcatct
     1081 tagaaaaaac cgtacaaaaa gttgtattgg aattaaaccc aactgcaaaa ccaatcgaaa
     1141 tgattgaacg atctggttca tcaactaaaa gcttttgagc ttcttttacc ctgaattcat
     1201 tcacaaatgt attaaaattc ttcttaatat gctcatttaa aatttcagaa agctgatgta
     1261 ctgttatacc taaatctcgt gccaggtcag gcaggctaag ctcttcatca gcaaatgcct
     1321 tttcatctac cattatctca taaagccgtt gtataacact atcaacatcc aatcctttga
     1381 ttcgtgaacg ctcataacgt gcttttaagg cttcattctt tagcaactta ttataatcgg
     1441 gaagacgctg tgttaccaga tatacaaacg tcatggctgt attggctaaa attactgccc
     1501 actcaaccac ccttaacgat ataaacactc ctataacata tacaatattt gaaataacta
     1561 ttgacatcgc aaagatgtat ccccagcgtg aaacgcctgg tactttgttc caattgctct
     1621 ttttccatat atacgacgtc ataaaaaggt tctgcgacat aaatgaaatg agatagatat
     1681 tggggaagat aaaaaacagg tacaatattg tatcatagag cgatagttca ttaaatgtat
     1741 ggctaagaat gggcaatgca ggaaaacagg tatatccaca gtcactatcg ataaaaaatt
     1801 ggatcattat atatagtaac gaaaccacta ctggtgaaaa taagaggtaa tatttttttt
     1861 gaaagttaaa caaacttgac attatccatg tatagcgtat taccataagc ggcggaacgg
     1921 taaatacaat gggtatggaa aagatacaca catagtaacc aacggcagtg ccatcaaata
     1981 accctgaaga aaatatggaa atctgtaaca accaaatccc cagccctaaa aatgaaaatg
     2041 cataaatcct gttgatggtt tcacttttct tttgtaaaag ctgacctata gcaataatca
     2101 ccgaaaacgc agcgccataa aacataaaaa aatgtaaaat agactcagcc atatattact
     2161 caaaatttta tagttataac attgaaatat agcgaacttt caattgtata cacttgaaag
     2221 atttcaggca taatatttga tataatcata acagttattg aataccatcc agtcgaaata
     2281 attgatatgg atgagatgaa actatcggag gtaattatat ggctcagcat gaaaatgtca
     2341 attatgaaaa agaatttgaa gaatatttaa aacgccagaa gcaccttgga ctcatggcac
     2401 ttgatcgtgc tgaaaagttt ggtgattcaa agattgcagt gcgtcataaa gcgtatggca
     2461 catgggaagc atttacatgg aagcagtttg gtgatttaat aaaagctgct gcaaaaggtt
     2521 tgctatctct gggcgttaag gagtttgagt ttgttggtat tttttcaaac aacagagttg
     2581 aatgggctgt tgtagattat gcatgcttta ccatcagggc atgttctgta ccggtgtatg
     2641 ctaccaactc agccaaagaa ttggaatata ttgtcaacga ttgcggcatc cggattcttt
     2701 ttgttgaaaa acaggaacac tatgacaagg caatgagtgt atttgacaat tgcaaaacgc
     2761 tggaaaagat tatagtgttt gatagaaata ctaacatagt caacagtgat aaagtaatgc
     2821 tctttgatga ttttattcaa ttagggaaaa attctggatt ggatgctcag ctacaggagc
     2881 gattatcaaa agtacagagc gatgatcttt ccaccctgat ttatacatca ggaactaccg
     2941 gcgagccaaa aggggttatg cttacccata aaaattggtt tgcaatgctg tttggtacag
     3001 gataccatat taccgttgaa cccactgatg taaaccttgc atttttacct ttatcacatg
     3061 tttttgagcg tgcatggagt tattttatat tgtgcaacaa tgcgcaggtt gattactgcc
     3121 acgatacaaa agcattactg gattttctga aagaatctcg tccccatttc atgtgcagcg
     3181 ttccacgaat gtgggaaaaa attcatgcta ccatcatgga tggtattaag aattcaccac
     3241 ctgctaaaca aaagatattc aactgggctc ttgaggttgg cggcaactat cagtatttaa
     3301 aaaaagataa taaatccatc ccactgggat taaggctaaa acatgccata gccaccaaac
     3361 tggtactgca taaaatcaga gatatttttg gtggaaggaa taaagtattt aattgcggtg
     3421 gttctgcctt ttctggtgaa atcagcgaat ttttctttaa agcgggtgta ttcattttac
     3481 agggatatgg tttaaccgaa tgttttgtga tatgtgtatc caatccacat cgcaataaat
     3541 ttggcacctg cggcccggtt gttcccctga tgcaggtacg aatctcagac gaaggtgaaa
     3601 tacaggctaa gggccccagc atgacacagg gatattggaa taaaccagaa ctcacaaaag
     3661 ccctttttac accggatgga tgggtaaaaa ctggagatgt tggatttatt gacagcgacg
     3721 gattcattac cgtaaccgac cgacttaaag acctattcaa aacatcaggt ggtaaataca
     3781 tagctcctca gcaaattgaa accttactca atgaagattt ttattttgaa caggttgctg
     3841 caattggtga tggcagaaaa tttgtatctg cattaattgt tcctgccttt gagccactta
     3901 aaaattgggc taaaagtaat aatattccat tcacatcaca cgaagacctt atcaaacatc
     3961 ccgcagttat acaattttat cgggaaagga ttgatgaatt aacaaaaaat ttgggtcagg
     4021 ttgaaaaaat taagaagttt actttacttc ccaatgaatt tactcaggaa aaaggcgaaa
     4081 taaccccaac acagaagata cgcagaaaag taatcaatga gcattacaaa gatatcattg
     4141 aacagatgta taaagattaa atcatcttta aaaaagctcc tgtgcacgca ggggcttttt
     4201 tattataaaa ataccagata tcctgataca gtataaatca ttataaatac attgacatat
     4261 ttattgtttt actgtataat tattgacatc ttaacaatct ttcataatgt tatgcatatt
     4321 aatatttata cactggatga aattacaaaa acctatgaaa atatagatga ccatctctcg
     4381 acacgacaga ttatccgtaa attttcacaa aacaaaaatg atatacggtt actatcgctt
     4441 gaactatgta acctatcaca ctacaacaat attttagatt tagggtgtgg ttacggcttt
     4501 tttaccgaag cgctcaacaa ccgcatagct tcaggcgctt gcataacagg tgttgatgtc
     4561 attggcggtg aaaacaaacg catgttttta tcaacctgtg tacaaagcgg atacaatgca
     4621 caatttatag aagattctgc acatataatc tgcaatattc ctgataaaac atacgattgc
     4681 attatagcaa gttattcact gtactttttc cctgatttaa taaaccacat cgcacgtatc
     4741 cttacaaatg atggaacatt tatagcactt acccatagtg aattaaccct ttcacaaatc
     4801 acatctattt ttattgaaag tattcaccat gttataccgc taagcgatag ctctctccat
     4861 atagcaaaat tatttaaatc attctctgct gaaaatggtt ttgtgcagct tcagccatac
     4921 tttgattata ttatgtatct gcgatatccc aataatctta tatttaaatc ttctgacatt
     4981 gaccactgta taagctatat acacaaaaaa caatatttat ttttcaagga tattgctttg
     5041 ttagataggg atgaatatga tgcattaatc aatgattttt ataatcgttt acgatggcat
     5101 gtatcatcat ataaggattt tattgtcaac aaggatgatg caatatttat tgctcgcaat
     5161 cccgttaagc acattaatga ataattttat tgatggtaca atacgtgtaa aattgatagt
     5221 gaataaaaag caataaaata ggtattatac catggtgaag tatattttgt tgttttataa
     5281 ggaactcaat tatgaagaaa aaacgcatct actgcagctt ttgcggtgca cccataacgg
     5341 cccgttttat tgatgaaaaa tatcgcgatc actgcgataa ttgcaacact acattctatg
     5401 aaaatccgct tcccgttgca tcatgtattg tcattaataa aaaccgtgaa gtacttcttg
     5461 tgctaaggaa aaacgaacca tataaagata tgtggtgttt accaattggg tttgctgaat
     5521 ccggagaatc aattgaacag gcagctctgc gcgaactaca agaagaagca ggtgttaatg
     5581 gtgagatagt tcgtattatt gatgttgata ctgtttcaaa ctacttttat ggtgatttag
     5641 caatcataac atttgaagta caacaacttt ctgaaacata taaaccgggt gatgatgctg
     5701 ctgatgcaaa atttttttcc cttgataatt acccgcaact ggcatgggaa tccaacgaaa
     5761 aagctttgca taaatttatt gatttatata aagatatatg gacaatgatt gattcattaa
     5821 aaactatcca acctgatata accacccatc atgatatccc cagagaaaaa tcaaaacaat
     5881 atacatttat tgccaacatc attgcatcaa tgattgatga tgaaattgaa ttgtttaccc
     5941 agcagtggaa acgtgagatg cccaatgtta ataataatat ttttcatttt ttgattttta
     6001 tccatcagat ggcgttgcat acaataaaac tatggctaac cggcaatagg atatggaaaa
     6061 atttcaggga attttatatc atgggaaaac agttaaaaaa agaaaatata tccttaaagg
     6121 atatattgag tgccatagct cttagcagaa aatgtatctg gattcaggca attgaaaaaa
     6181 atatattgca ttcaccactg gaagtgtaca caatgctgga gattaataac aggattattt
     6241 tgttttatga taagattagc tattttctta tcaagggata tgaacattca tagcctatta
     6301 ttcacagggc atcccatgag gatgccctgt gttatgtatc ttatgctgat tgtcttcgct
     6361 ttttttcttt gatttttgca agcaccttct cctgcatctc tttaggcagc gcaacatatt
     6421 tatcaaattc cattgcataa tttgcccttc cacttgaaag gcttcgcaat gttgttgcat
     6481 acccaaacat ctccatgagt ggaacaaaac cattgacctt ttgggcaccc tttctaaaac
     6541 gacgcattga ctctacttta ccccttcgcc tattcaaatc accaataaca tcgccaatat
     6601 attcatcagg ggtgttaacc tctattttca tctgtggttc aagcaatata ggtttagctt
     6661 tcataaatgc ttctttaaaa catatcgatg cacatgttct aaatgccata tcagatgaat
     6721 caaccgggtg gaaactacca tcaagcaaca caacccggac atcaacaaca ggaaaatcag
     6781 ccaatatacc ttcttccatt gctgcatgta ttcccttctg aattgaagga ataaattcat
     6841 tgggtattgc tccacctttt atatgatcta taaattcata tcctttcccg ggatttggtt
     6901 ctattcgcat aacacaatgt gcatactgac ccttaccacc ggtttgctta acaaattttg
     6961 tttcctgttc tgcttcagca gttattgttt ctcggaatgc aacggaaggc tcgccaacct
     7021 gtacattaag cccaaattct tctttgagcc tatcaaccat aatctctaaa tggagttcac
     7081 ccatccctga tattatcgtt tcacctgttt cttcgtcaac gcgcacatta aatgatggat
     7141 cttcagtggc taatttatgg agagctatcc ccagcttctc ttcttctttt tttgaagcag
     7201 gcataacctg taaatcaata actgatggtg gaacatatat cgattcaagc aatagcggat
     7261 tatttgtatc acagagggta tcgcctgttt tggtaaattt aagcccaatt aatgctacta
     7321 tctcacccgc tcccgcttct gaaatttctt ctcgttcttt tgcatgtatt cgcatgatac
     7381 gcccaatacg ctcatattta cccttggtag agttatatac ctgcatgcca ctttgtaatt
     7441 ttccagaata tatacgtatg aatgtttgct gaccaacata gagatcgttg attagtttaa
     7501 atgccaatgc agcaaatggt tcatgtgtcg aagggtgacg cgtatgagtt ttttcaggat
     7561 aatcaacatc aataccaact atcgctccac tatcaacagg agatggaaga taatcaacaa
     7621 ctgcatcaag caaaagttga atacctttat ttttatatgc agcaccacaa aatacaggcg
     7681 tgataagcat ctttaatgta gcttctcggg ctgtcttttt tatcagttca gatggcatct
     7741 ctttttctgc aagataaagc tccataatct catcatcaaa atcagccagc ttttcaataa
     7801 tatgtttacg cgcttccttt gcttttcctt tataatcatc cggtatatcc acaatcaacc
     7861 gctcgttttc cctgaaaact atcgcccgtt gctcaacaag atcaatgcag ccttcaaagc
     7921 tgtcttcgac accaatggga atctggaatg gaacagggtt tgcatcaaga tatctatcca
     7981 tctgagaaac aacttcataa aagtcagcac caactctatc cattttattg ataaaagcaa
     8041 ttcgcggaac tttatatcta tcagcttgat tccatacagt ttcactctga ggttctacac
     8101 cgccaacagc gcaaaataca gcaataagcc catcaataac acgcaatgaa cgttcaacct
     8161 caaccgtaaa atcaacgtgt ccgggggtat caataatatt tatgttatga tcgttccatt
     8221 gacatgatat ggaagctgaa gaaatggtaa taccacgctc ctgctcctgc ttcataaaat
     8281 ccattacagc ctgcccatca tgaacctcgc ccaattttct ggtaacaccc gtaaaatata
     8341 aaatacgctc ggtgacggtt gttttacctg catctatatg agcagcaatt ccaatatttc
     8401 taatattacg caacggcata ttttactcct tataaactat aaaatatctg tcagcaaaat
     8461 aattttaagc tgaccatgaa aaccagtagt aaataaattg atagcttttt atttaaccag
     8521 aggaagtacc actacactat ggttggtcat ggttataaag atagcaaaca tgtcaacatt
     8581 ttaaaaattt tttttatatt ttttaataaa acttaccatt tttataaaat taccaacctg
     8641 tatggcattt taacaacaat actggtggcg atagttactc ttaatgaacc ttttttaatg
     8701 caataacttc tattttataa aacaacacaa ttttttttaa aatgcgatgg caaccctgta
     8761 tctgcttaat aatatagtca cagtaaccca ctcacccttt tagaatatcc tgatatgaat
     8821 acaaaggttt tgagcgatac agtgcataac cctgtcaata aatttatagc aaaacatttt
     8881 tctgcttgaa atattttagc cgtgtaacta aaaacttatc atttcataac agcagtaaag
     8941 tatacccaac atcacctttt caggagggaa catatatgga taacaacgat aggtcacttt
     9001 ccatctttgg aaatcccatc cccaaacaca cctataataa agcaatgaaa caaaaagcac
     9061 gctttgcaaa aaaatttggc tttaacccca atgatcgcta tgaattgtcg gcactaccca
     9121 atcctatctt aaaaaaatat tgtaatctat acactgtttc acaggaagaa ggttctgaaa
     9181 tagcaaaatc aaaatctatt attattggta ccatacgcat gggatatggt cattatcgta
     9241 ttgctatggc aattgcttca gcagctcatt caatggggat tgtcccatac tggtttgatc
     9301 ttttatcatt tgatacagcc ggtgcaaaaa tcatcaaaca tatggataac ctgtattcat
     9361 ttggttcacg cttgtcacag caattttatc tttttaacaa gttatactgg gagcatctta
     9421 ctgccattgg atttaaaaag ctaacatata atgctactga ccaggcaatg actcagctat
     9481 ttgccaatat ataccaaaat ttgccgcaat ctgtaccatt tgttgcaacg catgcatggg
     9541 catcgcaggc tgctgtccat gccggtacaa aaaaagtcat taatatgatc cccgataact
     9601 ggcctcttgc acttcatctt tccgaaggtg ccatccatac agtacaaact ccatcagcat
     9661 actttggtta tcgcaccctc aaaaatatgg gtaaaaaaaa ccaaatactc aatccaatgc
     9721 caaaagatga tatttattac accggtcact acgttgatca tgaactggtt gccaatgttg
     9781 aaaaggattg caatgctcgt ttaaccaggc ttaaaaataa aaagccgcga cgatttttat
     9841 taagcattgg aggggctggc gctcagcaaa agttatgcat ggatattatc atgcactgca
     9901 tcccacttct tgaaaatgaa aaattaacac tcatcatcaa caccggtgat cacaaagcta
     9961 tttttgatat gatacttaat accggattta gtcccagagt tccaaagact tcctatacac
    10021 aatgggcgga aactgaaaaa ctggtcaaca ctttaaccac aagggatgta cccggattgc
    10081 acattgtata taatgaagat attttttcag ccgtttatgc caccaatata ctcatgagag
    10141 tatgcgattg tcttataacc aagccaagtg agctggcatt ttatccagtt cccaagctgt
    10201 ttgttgccca tgtaggtggg catgaaatgt ggggagcaat acgaggcgct gagattggcg
    10261 atagtactgt ggaatgtgaa accgtacagc atacattgca agcaatggat ttattaatct
    10321 atgatgatga tttactgaca ctgtattgca ataatattat caaagcaaaa agtattggta
    10381 tttacaatgg cgcttacgag gtaatcaaat tggcttttgg aaaataggca atataataaa
    10441 aatagagtac agttgcagga ttgtaaacta atcctgcaac tgttatggta attacatacc
    10501 gggaatagag gtaaatactc cccgggggtc tacatcattt cgtattattt ccagaatttc
    10561 ttttgatgga acttcagtta ccggtatgtc acctgctggt ttaacaagct caaatccaca
    10621 tgcaagctga cataaatcaa aagatacacc aggatgcaat gattttacac gcattcgctt
    10681 ggtttctggt tcaaaatcaa acactgcagc ctgcgtaatt actgttacag gacctttgcc
    10741 cagtaaacct gctttctgcc tgctatcacc accgtcaata aatcctggcg aagtaataaa
    10801 atcaaccctt ggaacaaact tttgaggtga ctgcaatcca acaaggatca tgttttcagc
    10861 aagcgaagaa agatcattat tgccgcctga acctgttaat ctatgatcag gttttaaaaa
    10921 atctttccca atcatgtgag tgttaacatt cccatacata tctatctgtg caaagcctaa
    10981 aaatgccttg tcaacataac cattataaat ctgaccaaat gaatacgaca tctccaatat
    11041 taacggtgat ttgcgccatg ttgcaggacc acccaccgac catggcatgc caccttccat
    11101 ggggtcctga gcacctgatt cataaacaag tactatattg ggagcatgag ttttgcgagc
    11161 cagaataccc gctaccattg gtaatccagt tccaatatac acaacttcat gatccttaat
    11221 ctcttttgct aatacatatg ctaacaattc cagcggattc attggtttat ctgatctatt
    11281 acccataatt cctccttatg ttcaaatagt aatagtttat agttattata cattttaata
    11341 aaatttcatc ttggtgatat cttctggcca atcaccctga aattcttcat aattgaaatc
    11401 aacaccttca tcttcattat catattcttc ggctttggtt aatcttcgtg attcagcaat
    11461 ccactttgat ccacctaact tttcaatgaa ttcgtattga tctttaacgc cgaataccca
    11521 gtaatcaaga aatttctgga aattttcatc tgacttcatt ccaaacctga aagccatttc
    11581 ccaccactgt cgtgaccagt aataacatcc aggcatctgc ccggggacag caccatacgg
    11641 aagttcaacc actgcatcaa catacatagc aggaatagat acgcctttat tattattcct
    11701 gatatcactt tctggaacta tctcttcagc ggtaattata agcttgcgag tagcagctgc
    11761 cattgcaatg tcgtttattg ccggaccaaa aaaccgtgca ttcccttttt tatctgccgc
    11821 atgtacatgt ataatagata catcagggta tattgcggga atatacgcaa cagggtcagg
    11881 atcactgcgc attggatttt gcattacctt tacataggga ttatatttta tcatatcaga
    11941 gcctaaaagc tgcttgctga aaacgcccgg caatcctaac tgcgctgctt taaacccttg
    12001 agccattgtg ccatgactcc attcagataa aattgttacc ttacctgcct ttaactgccg
    12061 tgtgtaattt ctgtcaatcc ccctcatctc agcgccaagg tacgaattgt gcgaatatcg
    12121 cgcgcatcct gcattaatca tgtacgaatg attggtattg ggtgaactac aaaactgcaa
    12181 attcttcttt tgctgtcgta taacctcaaa atatgattgg attgaggttc gtacataaca
    12241 aaaaccacca tcagtcatga tatcattatc atgtacatac tctgcaacag cctctttcag
    12301 tgacatccgc ttatctttac gtgatcgatc cttgcttaga tgccactttc gagcatgctc
    12361 aaaatctatc aacctctcaa gaacagaggt atctgtaatt ttgagatttt taactgcttc
    12421 ttcattaaag ctgaattttc ccatttacat cctcctgcaa atataaagta tttcaaaaat
    12481 ccattatata tcaataatta taacatacaa ttttatatat tac
//
LOCUS       DAOSOX010000059        12311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5132_length_12311_cov_7.871328, whole genome shotgun sequence.
ACCESSION   DAOSOX010000059 DAOSOX010000000
VERSION     DAOSOX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_12311_cov_7.871328"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLX22_05925"
     CDS             complement(<1..200)
                     /locus_tag="PLX22_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19470.1"
                     /translation="MKKVLATLVSLLFAASVFAQTGVQTKEAKKNVGQEKKIEVQQKV
                     EVKKEVKKEEKAQKQEQKKEAK"
     gene            321..833
                     /locus_tag="PLX22_05930"
     CDS             321..833
                     /locus_tag="PLX22_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT19471.1"
                     /translation="MIERETLINFYNLYSKEIFNYCYHLTGVYEAAEDVLQDCFINLI
                     EYSKKNVINSETVKSFLYKTAHNLAINYLKKNDKIFYLDDDNNYSSVDTIAETIANEE
                     MNNEILVAIQNLDVKTRSIFILKRDNNYTNEEIAKILNISERTVRRKLQQALSHIMVH
                     LKNKGFYDIL"
     gene            891..1988
                     /locus_tag="PLX22_05935"
     CDS             891..1988
                     /locus_tag="PLX22_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT19472.1"
                     /translation="MKHINYSTLLSYIKGELKHNDDDAIKLHVMQCQLCANNLKALGM
                     ITKTQRQYSPSPKILSNILDYYHQSLLTKSTFFDTFKAFIALHRRVFALATIIIAAGI
                     ALVLLSYRSNPITIPHVLYMADKSGHYIKSDTIKEGRRITIPETKWAMLVANNDIVFQ
                     LAGGVDMVIMRSHYSKNAGTKKFHYSLEKGAINIKTNHHHTSMHYEVKTPDANIQPLG
                     TEFYINVSPEGTHIYIVEGKVLLTNTATKVTIETEVGKLYTISQTQISSFDIEKYSLQ
                     WINDIDARFNNVAITDEDLGSSSILTSDVNNNETTTYQSQIDNTEKSNTQSSALNSQD
                     KEDKGNAKEMRNDMKHDEIKVMRKEAKQKHR"
     gene            complement(2014..2985)
                     /locus_tag="PLX22_05940"
     CDS             complement(2014..2985)
                     /locus_tag="PLX22_05940"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT19473.1"
                     /translation="MLYINIIKERSVKSLKENLVGALTVMLIVINTLFWACFLFSVAV
                     LKIIPLRFTKKYINTILDTIARLWIYCNNAGLKITRKIVWDVEGDNNLTTNSWYLVIA
                     NHQSWSDIVVLQKILTGKIPFLKFFLKKELIGVPVLGLAWWALDFPFMRRYSQSFLLK
                     YPNLKGKDIEITKKACQKFKDIPVSVMNFVEGTRFTKEKHTKQGSPYSNLLKPKSGGI
                     GFVFSTMGDYITSILDVTIVYPEGNKSFWDFLCGRITMIKVQIDEIPVTEALRGDYID
                     DPVFKRKFQQWLNNVWYEKDMRINIMKGKSFVPHNMHTEEDEAIIIN"
     gene            complement(3512..3808)
                     /locus_tag="PLX22_05945"
     CDS             complement(3512..3808)
                     /locus_tag="PLX22_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19474.1"
                     /translation="MKRITMIIIITVLSTVLLAKNAFFKGRVIFTNKDYIEVKYGKTE
                     KQFYVNDKSVFKKGGTNVTFADIKPCQIVKVVYAVADKTMYITTCEIIKESDCE"
     gene            4188..5399
                     /locus_tag="PLX22_05950"
     CDS             4188..5399
                     /locus_tag="PLX22_05950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT19475.1"
                     /translation="MELAHRLKKIKPSATLAVTALANSLKAQGVDVIGFGSGEPDFDT
                     PQSIKDAAIKALVDGKTKYTPSGGIPELKKAIVQKFKRDNNLEYKLSEVTVNCGGKHS
                     FYNLMQVLLNSGDEVIIPAPYWVSYPDIVLLADGTPVVIHTDELTNFKITPEMLKKHI
                     NKKTKAVVINSPSNPTGSMYTLDELKALGELLKNYDICIITDDIYESIVYNGNKFYNI
                     LNAIPELKSKTMVLNGVSKTYSMTGWRIGYMAGDETIIKQVEMVQSQSVSNPTSIAQW
                     AAVEAIMGDQSFINEMLQAFTRRKEMIVKGLNDINGVECHNPDGAFYVFPNVKKIYQC
                     NGFKHIIDNYKGDSLSSKLSSYLLEEARVAVVPGVAFGSDNNVRLSFATSDKSIIEGL
                     KRIQEAIEKLV"
     gene            complement(5441..5839)
                     /locus_tag="PLX22_05955"
     CDS             complement(5441..5839)
                     /locus_tag="PLX22_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOT19476.1"
                     /translation="MEEILQKANELGLMIKGSDIFKRYEELVKKIEADSNARTLLDEY
                     IKMSEEIYKKESSGGVVEVEEKQKLQDLSQKVSENQLIKEFIATQSYYVNMMMQIQNT
                     ISNPKGDPIGPSKIIKPGAGTTGKIVTGNF"
     gene            6235..6585
                     /locus_tag="PLX22_05960"
     CDS             6235..6585
                     /locus_tag="PLX22_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT19477.1"
                     /translation="MVEQKEIIEDENKIATVIASDISFRGTLKFKSSLKIKGSLEGKV
                     QTDGTLIIGDDARVSAEITAATVSISGKCQGKIKASKLIEIFEHSNMSGDIVTPDLYV
                     ENGAKFNGTCIMPK"
     gene            6614..7264
                     /locus_tag="PLX22_05965"
     CDS             6614..7264
                     /locus_tag="PLX22_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT19478.1"
                     /translation="MIFQKNLLKVSCVIVVFISSTLAIAQNNYSNYSFGFSLSGGADD
                     VWHKSGKDDGKDITWNQGIMAGAGLFFENMFTNSFGIHAGINYSYLESELVFQDSTDE
                     KLKSKNHSIIIPLYLLSSFGNTIRIDILYGLSYMHIFHNTMSSNNQHTDGIRYINYNQ
                     FGAGLMLRLVFAFNRFTYFYIAPHGQFYFTNVIEDTSWRDYLYTYQLEIGMIFKTF"
     gene            complement(7338..8114)
                     /locus_tag="PLX22_05970"
     CDS             complement(7338..8114)
                     /locus_tag="PLX22_05970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOT19479.1"
                     /translation="MRYIIDNKNPNTRKQQIRTMFDSIVEGYDRANRILSLGIDTIWR
                     KKMIQQIDCKQNDVVLDVCCGTGDLSKLIRNTGAKVISLDFSSAMINKGKKNGNIDDM
                     AVVADASRLPFKEATFSKVCVSFGIRNIPDIDIFLGEVFRVLQPGGMFVALEFTRPRN
                     PIVRLIYFLYLYTFLPMIGGIIAGNIKAYHYLSKTIHTFIDPPSLLAIQEKHGFVEIK
                     CNSMTLSIVTAYSSIKPKSLSSPLSLQHLNNLQHNRVIIH"
     gene            complement(8111..9181)
                     /locus_tag="PLX22_05975"
     CDS             complement(8111..9181)
                     /locus_tag="PLX22_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOT19480.1"
                     /translation="MNTVDSIINAAYNNKRLEPHEAVLLFDCDVATLGVLANYRREQI
                     VSGNEVGFVIDRIINYSNRCQAMCKFCAFHAQAGKIQSYDLTVDDILQKIDELVHIGG
                     TQVMLQGGLHPDYTLQDYVAMIKTIKQHYPDIYLHSFSPSEIVHIAKRSGSTIDAIID
                     TLQQAGLDSIPGASDLLVDDIRKKVSPRKATVAQWCEVVRSIKKHGMHTTATMTYGMG
                     ETKLQRIEHLDVIRKMQDETGCIMAFIPWSFAPKGTNMDTIIPATGIDYLKTVAISRI
                     FLDNVPYLHAGWLTEGMALAQIALAMGANDMGGILMEEKVVKATGVTTTTNIPQLCDV
                     IINAGKIPVHRDSMYTVLRRMV"
     gene            complement(9191..10000)
                     /locus_tag="PLX22_05980"
     CDS             complement(9191..10000)
                     /locus_tag="PLX22_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOT19481.1"
                     /translation="MKLGYINYLNCYPFYFNMFEKKPLPDVEVIPAYPSQLNAMIKEG
                     KLAMSPISAAAYAEGYNDLVLLPDFCLSSIGYVRSVILVSKKPIENLNHSTVGLSSAS
                     QTSVVLLKILLQKYYGLVPNYIPSDPYATLHHVDAKLVIGNEAMMHETEPLPYVYDLG
                     DLWLNKTGYPVVFAVFAVRNDALDSNEKTINAVCQSYHTSLLQLKNNAPELIACASLR
                     YPDIAYDIATYYTLLKFEFANKLKEALMFYFTSAHELGLLPYIPSLQFYGR"
     gene            complement(10013..11098)
                     /locus_tag="PLX22_05985"
     CDS             complement(10013..11098)
                     /locus_tag="PLX22_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOT19482.1"
                     /translation="MKNIHDEKLALIYNKVIAGVPVSNDDAYTMLTTNDIMGLGFIAN
                     TVKKEMHGNIIYYGVNMNLNYTNICLLRCPLCAFSRDKDDHDAYCLSHDEIVHKIKNA
                     ADIDEVHIVGGIHPDLPFSYYEEMIALIKSTRPDIHIVAFTAVEYDYMAKKFNIPLDE
                     LFQRLKKAGIDSIPGGGAEILANHIRTTIAPKKISGQRWLEVMATAHRNGMKSNATML
                     FNHIEDTADIVDHMCKIRDLQNTTGGFKTFVPLVFHEENTGVRARRKDPTGYDIIRIY
                     ATARIYLHNIPHIKALWMYVGEKLAQVLLQCGVDDIGATYYYEKVVHSAGAKTPAWGS
                     EPFLQHLIYDAGFKPVRVNASYEAIKK"
     gene            complement(11079..11681)
                     /locus_tag="PLX22_05990"
     CDS             complement(11079..11681)
                     /locus_tag="PLX22_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOT19483.1"
                     /translation="MIIVGITGASGALLGIRCIEELVYHNKQIAVIVSKSAWPIIHHE
                     MQTDCTSIISIVKQRTMQPELLSNIIEYSPDDFFAPIASGSNQFEATIVIPCSMKTVA
                     AIAHGYADNLIHRVCDIALKEKRQLVIVPRETPLSALHLYNLYTLSMYGASVVMPIPA
                     FYNFPETVDDVVNFIIGRVFDQLHIQHNLYRRWHYEEHTR"
     gene            complement(11764..>12311)
                     /locus_tag="PLX22_05995"
     CDS             complement(11764..>12311)
                     /locus_tag="PLX22_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT19484.1"
                     /translation="SRCIEWRDMMQEEYFKACFVDILNRTKQKYDFKLIAYCIMDNHI
                     HIVIHTTSTGAPIARIVQYIKARFAEMYNKITGRTGPFWNERYKDIIVQFAHDAFHYL
                     LWLLWYIAFNPVRKRRCDSPVKYRYSSIQCYVQEGADVGVPIDHHEYFLQLGDSFSER
                     VRRFLQYEEAYRKRYSIAQWV"
BASE COUNT         3945 a         2267 c         2040 g         4059 t
ORIGIN      
        1 tctttagctt ctttcttctg ttcttgcttc tgtgcttttt cttctttctt tacttctttc
       61 tttacttcaa ctttctgctg aacttctatc tttttttctt gcccaacatt ctttttagct
      121 tcctttgtct gcacacctgt ctgtgcaaaa actgaagctg caaacaacaa tgatactaat
      181 gtagctaaaa cctttttcat aaaatcctcc tgattatgaa tttgttataa aattttagtt
      241 gatgaaaata ttgacttact aactatttga tatatattac aatctgatac aaaaataacg
      301 gacatttttt tttatatttt atgatagaac gtgaaacatt aataaatttt tataatctat
      361 attcaaaaga gatttttaat tactgttacc atttaacagg tgtttatgaa gctgcagaag
      421 atgttttaca ggattgcttt atcaatttaa ttgaatattc aaagaaaaac gttatcaaca
      481 gcgaaacagt aaaatcattt ttatataaaa ctgctcataa tttagccatc aactatctaa
      541 agaaaaacga caaaattttt tatttagacg atgataacaa ttattcatca gtagacacaa
      601 ttgcagaaac tatcgccaat gaagaaatga ataacgaaat attagtagca atacaaaatt
      661 tagatgtaaa aactcgatca atatttatct taaaacgtga caataattac acaaacgaag
      721 aaattgcaaa aatactcaat atctctgaac gtacagttcg cagaaagtta caacaagcat
      781 tatcgcatat catggtacat cttaaaaata aaggatttta tgatatttta tgaatcatat
      841 gaccattttg cttattttaa ttgtattata attaaggggt tattaatgcc atgaagcata
      901 tcaattatag tacattgctg tcatacataa aaggtgaatt gaagcataac gatgacgatg
      961 caataaagct tcatgttatg caatgtcagc tatgtgctaa caacctcaaa gcattaggta
     1021 tgattacaaa aactcaaagg caatattccc cttcacctaa aatattatca aatattctgg
     1081 actattatca ccagtcatta ttaacaaaat ctaccttttt tgatacattc aaagcgttca
     1141 ttgccttgca tagaagagta tttgcactgg caaccattat tatagctgca gggatagcac
     1201 ttgtattgtt aagttatcga tcaaatccca ttactatacc acatgtatta tatatggcag
     1261 ataaatcagg gcattatata aaatctgata caatcaaaga aggacgtaga attaccatac
     1321 ctgaaactaa atgggctatg cttgttgcta acaatgatat tgtttttcag ttagctggtg
     1381 gagttgacat ggtcataatg agatcgcatt atagtaaaaa tgctggtact aaaaaatttc
     1441 attattcact tgaaaaaggt gctataaaca taaaaactaa tcatcaccat acatcaatgc
     1501 attatgaagt gaaaactcct gatgccaata ttcagccatt gggtactgaa ttttatatta
     1561 atgtttcgcc tgaagggaca catatttata ttgttgaagg taaagtacta ttaaccaata
     1621 ctgcaacaaa agtaacaatt gaaaccgaag ttggtaaatt atacaccata tcgcaaactc
     1681 agattagttc atttgatatt gaaaaatact cattacaatg gatcaatgat attgatgccc
     1741 ggtttaataa cgttgccata acagatgaag atttaggttc atcatcaata ttaacatctg
     1801 atgtaaataa taatgaaact acaacgtatc aatcacaaat tgataatact gagaaatcaa
     1861 acacccaatc ttcagcatta aacagtcaag ataaagaaga taagggtaat gcaaaagaaa
     1921 tgcgaaatga catgaaacat gacgaaataa aagttatgcg aaaagaagca aaacaaaaac
     1981 acagataata ttagctatat ttaatctgta tggttaattt ataatgatcg cttcatcttc
     2041 ctcggtatgc atattgtgtg gcacaaatga tttacctttc ataatattaa ttctcatgtc
     2101 cttttcatac catacattat tgagccattg ctgaaatttt ctcttaaaaa ccggatcatc
     2161 tatataatca cctctcaatg cctctgtcac tggaatttca tctatctgaa cttttatcat
     2221 agtaatccta ccacataaaa aatcccagaa tgatttgtta ccttcaggat atacgatagt
     2281 aacatcaaga attgatgtaa tgtaatcacc cattgttgaa aaaacaaaac caatgcctcc
     2341 tgatttaggt tttaaaaggt tactatatgg tgatccctgc tttgtatgtt tctcttttgt
     2401 aaaacgtgtt ccctcaacaa aattcataac cgatacaggt atatccttaa acttttggca
     2461 ggcttttttt gttatctcaa tatctttgcc tttcagattg ggatatttta ataaaaatga
     2521 ttgcgaataa cgtctcataa acggaaaatc taaagcccac catgcaagtc ctaaaactgg
     2581 aactccgata agctcttttt ttaaaaagaa ttttaaaaat ggaattttac cggttaatat
     2641 tttttgtaac acaacaatat cgctccatga ctgatgatta gctattacta aataccagct
     2701 atttgttgtg agattattat caccttcaac atcccatact atttttcttg taatttttaa
     2761 accagcgttg ttacaataaa tccataaacg ggctatggta tccaatattg tattaatata
     2821 cttttttgta aatctcaacg ggatgatttt tagcacagca actgaaaata aaaagcaagc
     2881 ccagaataat gtgtttatta caatcaacat aactgttaaa gcaccaacaa gattttcttt
     2941 taagctcttc accgatctct ccttaattat atttatatat aacattattt gtaataattt
     3001 actggtttac tgttatacca ttctttataa gtagtagaac caaagtgtat agtaaagaca
     3061 gcagtatggg tattttggtt gcaaatttat ttttaaacta taaaaatttt acattatttt
     3121 ggataatgtc aaatggtatc atgttcttgc gaatagcatg cacctgtaat tatttcatgt
     3181 cttgtcattg gattgttgtg gcaatactac aaacaccatg cttatcctct atttttatta
     3241 catgggggcg atagctcctg tgaaacaata tcagcatatc atggcaacag tgagcataca
     3301 tcatctgcat tccgcttgca cattgacaaa acgataacct tttttgtgca tatcaacatt
     3361 ctgacatgtc atgcaattca aatattttga gcgataatta tatccctgcg gcatatactg
     3421 taaaattgat tactggcagg cacttatcaa tgcaaaatag attgttatga agtcactatc
     3481 gccaaaaaaa atagcctatg agattataca atcattcgca atcgctttct ttgattattt
     3541 cacatgtcgt tatatacatt gttttatcag cgacagcata aactactttt actatctgac
     3601 atggtttgat atccgcaaac gttacatttg ttccaccttt tttgaataca cttttatcat
     3661 tgacataaaa ctgtttttca gttttgccat acttaacttc aatataatct ttattggtaa
     3721 atattacacg acctttaaag aatgcatttt ttgctaataa cacagttgac agtactgtaa
     3781 ttatgattat cattgttatc cttttcatat catttacccc ctcgtattga taattgcacc
     3841 caattacctc taaatagttt tgcaatcaca gcactattac tatagatgtc atataaacaa
     3901 tatgcaacta tattttatga acataatcac cgtgctatct caggtcaaag tgtaaacaaa
     3961 tatactatat atgtcaatga ctatttatta taagtttttt tgaatttact taaattggta
     4021 atttttaaat atttatattg tttttaatgc ataaatttct tgacaaaata agattatagc
     4081 tttgttgttc tttttgatat aaatcagatc tcccacatac tacgtttttt ctactccctt
     4141 tttttatcaa atattgtgat attctatata ccatgaaagg agaagttatg gagttagctc
     4201 atagattaaa aaagattaaa ccttcagcta ctttagcggt aactgcgctt gctaattctt
     4261 taaaagcaca gggagttgat gttattggat ttggttccgg cgaacctgat tttgatactc
     4321 cccagtcaat taaagatgca gcaataaaag cattagtgga tggaaaaacc aaatacacac
     4381 cttccggagg tattcctgaa ttaaaaaaag ccatagttca aaaatttaaa cgggataata
     4441 acttagaata taaattatct gaggtaactg ttaattgcgg cggaaagcat tcattttata
     4501 acctcatgca ggtgttgctc aatagtggtg atgaggtgat tatacccgca ccctactggg
     4561 tttcatatcc cgacattgta ttgttagctg atggaactcc cgttgttata cataccgatg
     4621 aactaacaaa ttttaaaatt acccccgaaa tgttaaaaaa acatatcaac aaaaaaacaa
     4681 aggcggttgt tatcaattct ccttcaaatc ctactggctc catgtataca ttagatgaat
     4741 tgaaagcatt aggtgaattg cttaaaaatt atgatatatg tatcataact gatgatatct
     4801 atgaaagcat tgtatacaat ggtaacaagt tttataatat tctcaatgca atacctgaat
     4861 taaaatccaa aactatggtg ctcaatggcg tttcaaaaac ctattccatg acaggctggc
     4921 ggattggtta catggcagga gatgaaacca tcattaaaca ggttgaaatg gtacaaagcc
     4981 aatccgtttc caatccaaca tccattgcac aatgggcagc agtagaagct attatgggcg
     5041 accaatcatt tattaatgaa atgttacaag catttaccag aagaaaagag atgattgtca
     5101 agggtcttaa tgacattaac ggtgtggaat gccataatcc ggatggagca ttttatgtat
     5161 tccccaatgt aaagaagata tatcaatgta atggctttaa acacattata gataactata
     5221 aaggtgattc gttatcgtca aaattatctt cttatttact tgaagaagcc agagttgcag
     5281 ttgtacctgg cgttgcattt ggttcagata ataatgtaag gttatcgttt gcaacatctg
     5341 ataaaagtat catcgaagga ttaaaacgga tacaagaagc tatagagaaa ctggtataat
     5401 aacaaccacg cagagacgac agatatggtt gtctctgctg ttaaaagtta ccagtaacta
     5461 tcttcccggt agttccagca cccggtttta ttattttaga tggccctatt ggatcacctt
     5521 ttggattgct aatggtattc tgaatctgca tcatcatgtt aacataatag ctctgagttg
     5581 caataaactc tttaataagc tgattttctg ataccttctg gcttaagtcc tgcaactttt
     5641 gtttttcctc aacttcaact acaccacctg atgattcttt cttatatatc tcttcactca
     5701 tttttatata ttcatctaac aatgtgcgtg cattactatc agcttcaatt ttttttacca
     5761 attcttcata ccgcttaaaa atatcactgc ccttaatcat tagccctaat tcatttgctt
     5821 tttgtaaaat ttcctccata caatatttct ccttcattat tatcgcttca atagtttttg
     5881 ataaaattgt tattctctat agagcagatt tactatacaa tacattatag catatattac
     5941 actttctttc tataataatc tatgatggat ttatgcaagg attttttttc ttttttttta
     6001 aaaatatgct atttctgata aattgaattg acattacagc agtaatgata aaatatttta
     6061 attttaatga gtttacaatg tatagcattg tgcctgagaa attgggatac atatatttgg
     6121 cgacagtcat ttgaattccg gaacaagtcc ggaatgacga aggcggcgaa agcagattcc
     6181 gggtaacgcc tggaataaca ttgagaggat aattctattt tcggaggata caatatggta
     6241 gaacaaaaag aaattataga ggatgaaaac aagatagcta cagtaattgc aagcgatatt
     6301 tcattccggg gtacattgaa gtttaaatca tcattaaaga ttaaaggttc attagaagga
     6361 aaagttcaaa ctgacggcac gctcattata ggagatgatg ccagggtaag tgcagaaatt
     6421 accgctgcta ctgtttcaat aagtggtaaa tgtcagggta aaatcaaagc ttctaaactt
     6481 attgaaattt ttgaacacag caatatgtca ggtgacatcg taacgcctga tttgtatgtt
     6541 gaaaatggcg caaagtttaa tggcacctgt attatgccaa aatagtataa aacccataat
     6601 aggcaactaa aacatgattt ttcaaaaaaa tttgctgaaa gttagttgtg ttatagttgt
     6661 ttttatatca tctactttag ctatagcaca gaataattat tcaaattatt cctttggatt
     6721 ttctttatcc ggtggagctg atgatgtatg gcataaaagt ggcaaggatg atgggaagga
     6781 tattacatgg aatcaaggta ttatggctgg tgctgggtta ttttttgaaa acatgtttac
     6841 gaatagcttt ggcatccatg ctggtataaa ctattcatac cttgaaagtg aattggtatt
     6901 tcaggatagt actgatgaaa aattaaaatc aaaaaatcat tctataataa taccattata
     6961 tttgctatca tcatttggca atacaattcg tattgatatc ttatatggat tgagttacat
     7021 gcacattttt cataatacca tgagcagcaa caatcaacat accgatggga ttagatatat
     7081 caattataat cagtttgggg caggattaat gttgcgattg gtatttgcat ttaaccgttt
     7141 tacctatttt tatatagcac cacatggcca attttacttt accaatgtca ttgaagacac
     7201 aagctggcgt gattaccttt atacctatca acttgaaatt ggtatgatat tcaaaacatt
     7261 ttgatcctga ttcccagact ttatctacta attgaatggt tactgataaa aaaacactga
     7321 tgaaacatca attctatcta atgtattatc accctgttgt gctgtaagtt gtttaaatgc
     7381 tgtaagctta aaggcgatga tagggacttt ggttttatac ttgaatatgc ggtaacaata
     7441 ctcagtgtca tggaattaca ctttatttca acaaaaccat gtttttcctg aattgccagt
     7501 aatgatggcg gatcaataaa tgtatgtatg gtctttgata gatagtgata tgcttttatg
     7561 ttccctgcga taattccacc aatcataggc aggaatgtat agagatataa aaaatagatt
     7621 aatcgcacaa taggattgcg tggacgggta aactccaatg caacaaacat ccctccgggt
     7681 tgtaacaccc tgaatacttc gcccagaaat atatcaatat ccggaatatt gcgtatgcca
     7741 aaggatacac ataccttgct aaatgttgct tctttaaacg gaagccttga agcatcagct
     7801 actactgcca tatcatctat atttccattc tttttgccct tgttaatcat ggcagatgaa
     7861 aagtctaatg atatgacctt tgctcctgta ttgcggataa gttttgacag atcacctgtt
     7921 ccacagcaaa catccagaac aacatcgttt tgcttacaat caatttgctg aatcattttt
     7981 ttacgccaga ttgtatcaat ccctaatgat aaaatcctgt ttgccctatc gtaaccttca
     8041 acaattgaat caaacatggt cctgatttgt tgtttgcgag tgttgggatt tttgttatca
     8101 atgatgtatc tcataccatt ctccgcagta cagtatacat ggaatctctg tgaaccggaa
     8161 ttttgcctgc attaatgata acatcacaca actgcggtat atttgttgtg gttgttacac
     8221 cggtagcctt tacaaccttc tcttccatca atatgccacc catatcattt gcacccattg
     8281 ccagcgctat ctgtgccaga gccatccctt cagttagcca tcccgcatgt aaataaggta
     8341 cattgtccag aaatattcgt gaaatagcaa cagtttttag ataatcaatc cctgttgcag
     8401 ggattatagt gtccatattt gttccctttg gtgcaaatga ccatggaata aatgccataa
     8461 tacaacctgt ttcatcctgc attttccgta taacatcaag atgctctatc cgttgaagct
     8521 ttgtttcacc catcccatag gtcatggtag ctgttgtgtg cataccatgt ttttttattg
     8581 aacggactac ctcgcaccac tgtgctactg tagctttgcg cggacttacc tttttacgga
     8641 tatcgtcaac cagaagatct gatgcgccag ggattgaatc caaccctgcc tgttgtaaag
     8701 tatctataat agcatcaatt gtactgccgc tgcgctttgc aatatggact atctctgatg
     8761 gtgaaaatga atgaagataa atatcaggat aatgctgttt gatagttttt atcatggcaa
     8821 cataatcctg taatgtatag tcaggatgca atcctccctg cagcattacc tgagtgccac
     8881 ctatatgtac cagctcatca attttttgca aaatatcatc aaccgtcaga tcatatgact
     8941 gtatcttccc tgcttgtgca tggaatgcac aaaatttaca cattgcctga cagcgattag
     9001 aataatttat aatccggtct ataacaaagc caacctcatt gccggagact atttgttctc
     9061 tgcgataatt ggctaaaact cctaaggtcg caacatcgca atcaaaaagt aaaacggctt
     9121 catgaggctc caggcgtttg ttgttatatg cggcattgat tattgagtca acggtattca
     9181 tatacagtaa ctatcgccca taaaattgta acgaaggaat atatggcagc aagcccaatt
     9241 catgagcact ggtaaaataa aacatcaacg cttctttgag cttgtttgca aattcaaatt
     9301 taagcagtgt atagtatgtt gcgatatcat acgctatatc aggatagcga agactggcac
     9361 atgcaattaa ttcgggtgca ttgtttttaa gttgtaaaag cgatgtgtgg tacgattggc
     9421 aaactgcatt gatagttttt tcattgctat ccagtgcatc attgcggact gcaaatacag
     9481 caaacactac aggatatcct gttttgttaa gccataaatc gccaaggtca tatacatagg
     9541 gaagcggttc ggtttcgtgc atcattgcct cgttgccaat aaccagtttg gcgtcaacat
     9601 ggtgcagtgt tgcataagga tcagatggaa tatagtttgg gaccagcccg taatattttt
     9661 gtaataaaat ttttaacagc actaccgatg tttgcgatgc actcgataac ccaacggtgc
     9721 tgtggtttaa attttcaata ggttttttgc tcaccagtat gactgagcgc acatacccaa
     9781 tcgaacttaa acagaagtca ggtaacagta caaggtcgtt atagccttct gcatacgctg
     9841 cagcagatat aggactcatt gcaagcttcc cttcttttat cattgcgtta agctggcttg
     9901 gatatgccgg aataacctca acatcaggaa gtggtttttt ttcaaacatg ttaaagtaaa
     9961 aaggataaca gtttaaataa ttaatatacc ctaatttcat ggtacctcgt tgctattttt
    10021 ttattgcttc atagctggcg ttaacccgca caggtttaaa accagcgtca taaattaagt
    10081 gctgcaaaaa aggctctgac ccccatgcag gcgtttttgc acctgcactg tggaccacct
    10141 tttcataata atatgtagca ccaatatcat ccacaccgca ttgcagtaac acctgtgcaa
    10201 gcttttcacc tacatacatc cacagcgcct ttatgtgggg aatattatga agatatatgc
    10261 gcgcagtagc ataaatacgg atgatgtcat agccggttgg atctttccgt ctggctctga
    10321 cccctgtatt ttcttcatgg aatacgaggg gcacaaaggt tttgaatcca ccggtagtat
    10381 tctgcaaatc acgaattttg cacatatgat caacaatatc agcagtatct tcgatatgat
    10441 taaaaagcat ggttgcattg cttttcattc catttctatg tgctgtcgcc ataacctcca
    10501 gccaccgttg tccacttatt ttttttgggg cgatagttgt tcggatatga ttggcaagta
    10561 tttctgcacc tccccctggt atggaatcaa tgccagcttt ttttaatcgc tgaaatagct
    10621 catcaagcgg aatattaaat ttctttgcca tatagtcata ttcaacagca gtaaaagcaa
    10681 caatgtgaat atcaggcctg gtagatttta tcaatgcaat catctcttca tagtatgaaa
    10741 aaggcaaatc gggatgtatc cctccaacga tgtgcacctc atcaatgtca gcagcatttt
    10801 ttattttatg aactatctca tcatgtgata aacaataggc atcgtggtca tccttatcgc
    10861 gtgagaaagc acataaagga cagcgcagca aacatatatt ggtgtaattg agattcatat
    10921 taacaccata gtagatgata ttgccatgca tttccttttt tactgtatta gcaatgaagc
    10981 caagtcccat gatatcattg gtggttagca ttgtgtatgc gtcatcgtta ctgactggta
    11041 ctcccgcaat aactttatta taaataaggg caagtttttc atcgtgtatg ttcttcataa
    11101 tgccatcgcc tatagaggtt atgctgaata tgtaactggt caaataccct gcctatgata
    11161 aaatttacta catcatcgac ggtttcagga aaattataaa aagctggtat gggcattacc
    11221 actgaagccc catacattga taatgtatag agattataaa ggtgtagtgc actcaaaggg
    11281 gtttcacggg gcactataac aagctgccgt ttttccttca gtgcaatatc acatacccta
    11341 tggattaaat tatctgcata tccatgagcg atagcagcca ctgttttcat tgagcagggt
    11401 ataactattg tagcttcaaa ttgattactg ccacttgcta taggtgcaaa aaaatcatca
    11461 ggactatact ctataatatt acttaacaat tccggttgca tagttcgttg tttaacaatg
    11521 cttataatcg aggtacaatc tgtttgcatc tcatggtgta tgataggcca tgcgctcttg
    11581 gatactataa cagcaatttg tttattatga tataccagct cttcaataca gcgtatgcct
    11641 aatagtgccc cgctagcacc tgttatacct acaattatca tgtgattaat tgtatcacga
    11701 agctgtgtat aaataaagat taattttacg gttgaggtat aaggctctga cccctttttt
    11761 attttatacc cactgtgcaa tggagtagcg cttacggtac gcctcctcat actgcaaaaa
    11821 acgtctcacc cgttcgctaa aactgtcccc cagctgcaga aagtactcat gatggtctat
    11881 cggcacacca acatcagcac cctcctgtac atagcattgt atgctgctgt agcgatactt
    11941 gacaggacta tcacaccggc gcttccgtac tggattgaac gctatgtacc acagtaacca
    12001 caacagataa tgaaacgcat catgggcaaa ctgtacaatg atatccttat agcgctcatt
    12061 ccagaatgga cccgttctac cggttatttt attatacatc tcggcaaacc gtgccttgat
    12121 atactgcaca atgcgtgcta tgggcgctcc tgtgctggtt gtgtggatga caatatggat
    12181 gtgattgtcc atgatgcagt aggctatcag cttaaagtca tatttttgtt ttgttctgtt
    12241 caatatgtcg acaaagcagg ctttaaagta ctcctcctgc atcatatcac gccactcgat
    12301 acagcggctc a
//
LOCUS       DAOSOX010000060        12300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5141_length_12300_cov_8.267129, whole genome shotgun sequence.
ACCESSION   DAOSOX010000060 DAOSOX010000000
VERSION     DAOSOX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_12300_cov_8.267129"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLX22_06000"
     CDS             complement(<1..1302)
                     /locus_tag="PLX22_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT19485.1"
                     /translation="MNSPDRYFLQPYQNNDIVLRLKAWAALIFNFSVIFAVVIMGTFF
                     AVRGIKDPGILYPFTGTIFSAIISLALLRKGKFQFAANLSFASVQIALWLVIFFESGS
                     LLQRMDSIVLISASLAYTPLLFVDRRRGIWFYYTANMLLLIIFVLYVQAALGLERWEV
                     IEYFMDSALALTFVTAISYQIFSIHKLGIINAYKAQNKIQKQYEELSATAEELEAMNE
                     ELTAINEELENTQQELLISNSNLQKETAQLQTILSSITDGIITTDDKGTITSINYAAL
                     KLFGFKDDVIGKQFASFCILYNAKTGIPYEDPVTQVLRQGKPVSLYRNTMIRNNGDTY
                     YVYIVCNPLYIANKIAGCIVVIRDITDLVRYEDEMIKTAKYESLSIFAGGIAHDFNNM
                     LNAILGNISIIEHLSPENERIIKVAGRAQKAIFKARDLTTQL"
     gene            1589..2011
                     /gene="umuD"
                     /locus_tag="PLX22_06005"
     CDS             1589..2011
                     /gene="umuD"
                     /locus_tag="PLX22_06005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOT19486.1"
                     /translation="MCPLPVINCSSRSPIAPLYATAIKAGFPSPADDYIETVLDLNEY
                     LIRHPAATFFVRVSGDSMTPGICHGDILVVDRALTPVHNNIVIAAVNGELTVKRLVIK
                     NGAALLVPDNDTYEAMALTPEMDCTIWGVVIHIIRTVG"
     gene            2014..3270
                     /locus_tag="PLX22_06010"
     CDS             2014..3270
                     /locus_tag="PLX22_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOT19487.1"
                     /translation="MYALVDCNNFYASCEKVCNPALWGKAVVVLSNNDGNVIARSPEA
                     KALGIAMGAPFYQCTDIIRKHHVAVFSSNYALYGDMSRRVMDTLATCAPYMEVYSIDE
                     AFLLCNVPPCHAEGYALQVKHTVLQWTGMPVSVGMGATRTLAKLANHIAKKRTQSGVF
                     VFVGDSSLYKNLLVHIDVQEVWGIGGRYARMLRRHGIDTVWQLICADDSFIKKTMTIV
                     GLRTVYELRGIPCISTEDAPPPKKCIVSSRSFGKPVTECNELQEAAAHYASIASQKLR
                     KQHGAAGVVTVFVSTNPFKDEPQYSSAATITLDMHSCYTPDIIAAAQTALHRLFRSGY
                     RYKKVGIMLTDIVDAQELSPTLFSPAQSCRKRYNLMQTLDSVNAARGRGTVYFASEGV
                     QRQWAMRRRYLSPRYTTHWDEIPLVR"
     gene            3365..3802
                     /locus_tag="PLX22_06015"
     CDS             3365..3802
                     /locus_tag="PLX22_06015"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT19488.1"
                     /translation="MMHYEPIPDTVMSYIPDITKILQDEAIIFAYLFGGLARGFYTPL
                     SDIDIAIYIDDVPDVADYTFNLFCKLAEAVKTSEIDIVILNTAPESITGRIVLNKVIL
                     TDKEPLKRYIYESLTLRKYFDFKMKEEQILFRRYNIGRYTINS"
     gene            3777..4040
                     /locus_tag="PLX22_06020"
     CDS             3777..4040
                     /locus_tag="PLX22_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT19489.1"
                     /translation="MVDIPLILKKLSKLEEYYKQIEEFKGVTLNEYSNDWKIQRIIER
                     TLQIMIETCVDIANHIIADNKLRIPESYADTFKVLHENTISIR"
     gene            4054..4230
                     /locus_tag="PLX22_06025"
     CDS             4054..4230
                     /locus_tag="PLX22_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOT19490.1"
                     /translation="MNKMVKFRDVVVHQYSNVNAEIVIAILKKHREDFMKYRDAIVMY
                     LKNHGFNEYHQNNA"
     gene            4242..4529
                     /locus_tag="PLX22_06030"
     CDS             4242..4529
                     /locus_tag="PLX22_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19491.1"
                     /translation="MIDITVIIILVVIATVLLWTVVLFKIVMSPFAIRDKWKLLGVLL
                     IFALCYSILPSWATKTLHLKSYTIPCIIVSILFVGLVILYTMINARLKKYK"
     gene            4671..5813
                     /locus_tag="PLX22_06035"
     CDS             4671..5813
                     /locus_tag="PLX22_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19492.1"
                     /translation="MILERKYDIEEIQKRIRYYPVTAIIGPRQSGKTTLAGMLRPDMI
                     FDLENPVDMAALENPVLQLDKEKGLIIIDEIQLKPELFPVLRYLCDKNPECRFCILGS
                     ASPDIIKQSSESLAGRISFYVLMGFRKEDLPHSLLEHFHFRGGLPRSLLAPDDTTSVM
                     WRKDYVKTFLERDIPLLGIRIPPYTLLRFWQMLAHYHANVVNFSEIGRSLEISDTQVR
                     RYIEILAGTFMVRLLQPWYANIGKRLVKRPKLYIRDSGILHSLLMIESKDALLRHPKL
                     GASWEGFALEAVVRSIKKNDDEIFFYGVHSGAELDMVWQHGGYAWGCEFKFSDAPVLT
                     KSMHNAVQDLQLKKAWIVYPGQKKYKVKNTIEVIPFDDIPDVWVYD"
     gene            6146..7270
                     /locus_tag="PLX22_06040"
     CDS             6146..7270
                     /locus_tag="PLX22_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19493.1"
                     /translation="MKKLIVVLVIAGVVIAQPLFAATITGTAPQLRVYVGGSWNTIDV
                     STEFNQAILDANAELAKYDDQDKLATGFANANMYSTNAATQQGYPNYSLFAITTGVMV
                     GAQAPTTNVDYYTNGKIEDDIENKGDLYAGVAGSAAVNVGINAGMIIPGLYINVKFGK
                     FDSTWVYDDDDFSCDTMIIGGGITYSLFDEAGTGLLRWRGLTIGTGLVYQTTDVSYRV
                     DLDSKTDSFTVDPGTGTDVTGSVVVDPSFDITLDMYTLSVPFEINTALQFLWLFNINL
                     GVGIDLVSGNADLELQSVSDATVKNIAYGGTPLPENEYIVTPGKVTIDGKTLDKKPSW
                     ARARIMTGIGLNFGAVKIDVPIIYYLKSGAAVGVTAGIIW"
     gene            7929..8189
                     /locus_tag="PLX22_06045"
     CDS             7929..8189
                     /locus_tag="PLX22_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19494.1"
                     /translation="MKKIMIFIMVILLSGVCFNVSHCIAESALDQAKDAAESSQKAKE
                     DADKGKYEEAREKAGGQFDDEYDREKDQEYPIPEPEISNEDD"
     gene            8447..9670
                     /locus_tag="PLX22_06050"
     CDS             8447..9670
                     /locus_tag="PLX22_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT19495.1"
                     /translation="MRIYTMKLISFFTWLFIFTGYCFSQELSPITIVATGNNASGFAL
                     FKQIDGKQKDKNIFISPFSIAMAFGMAYEGASGNTAREIASVFAFIPDDKDRWNAFST
                     IYKTLNPDNAKYKLAIANAFWVQQDYPLLKTYTDILKNYYHSEAYNVDFTHDDKRKEA
                     VKKINAWAANNTYDKIREAITLDDVKPLTRLILTNAVYFKANWLYQFDKNQTKVDNFY
                     IDSNKKIKTPMMMQRNVTIKYMENNNIQMIELPYEGKALSMFVILPKKDIKSIMPLLN
                     DKNFESWRQNLIQEEVDVYLPKFKVECGYMLVEPLIKMGIKDAFNEMKADFSKITGGK
                     DLYISKVIHKTFCDVNEEGTEAAAVTAIVMNTKSMVSFEKKIFKADRPFIYAIVDNNT
                     GVILFLGKMIKPEYK"
     gene            complement(9777..10160)
                     /locus_tag="PLX22_06055"
     CDS             complement(9777..10160)
                     /locus_tag="PLX22_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19496.1"
                     /translation="MKTVKKQIIAIFIATVWISVSEFARNEFIVKSYWIQHYNKLGLV
                     FPSQPINGAVWGIWSLCFAIAIFFIAKKFSLIQTTFLSWFAGFILMWLVSWNLNVFPI
                     AILPFAIPLSLLEAFLASFIVKKLT"
     gene            complement(10298..10516)
                     /locus_tag="PLX22_06060"
     CDS             complement(10298..10516)
                     /locus_tag="PLX22_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19497.1"
                     /translation="MLHIPIGQKLSLDEIVSLNVTIAIMYNRLSKRTLMRDVKELIDL
                     ELLLFDNNTYYANIDLLKKNISHRKKKR"
     gene            10914..11537
                     /locus_tag="PLX22_06065"
     CDS             10914..11537
                     /locus_tag="PLX22_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19498.1"
                     /translation="MMKKITTILIVAFFSIYFMGNSLYSYQKKYIFKDQTIIDEKTGI
                     TIMTNETKDLIIVTNYILQYAIIFPYNNQWEFDINDKYAIIGNCGTINISLQIHENNN
                     KTTEEFLSDLMKIYTENKSKCGVIDAKLIQYNKDLVLRTVVDPTIIYKDDTFKNVKHI
                     NYYSTKTFNNIRYVYHSSVIVDNDKKINEEDFLNFATRGFFIDFGRK"
     gene            11865..>12300
                     /locus_tag="PLX22_06070"
     CDS             11865..>12300
                     /locus_tag="PLX22_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19499.1"
                     /translation="MENDLKALKRKAKALLLSKDIPAGKMDIVRALLANDRVLEEEKY
                     RAIIDLVKNYPDKPVMPVSPGKVEVKGADEFKKTIQYDSKNTNVLYHKYKHTRLFKKR
                     LLVHANNKFGIGFLRRLIPSQKFLKVSQRLFDLQSALRQRLYK"
BASE COUNT         3903 a         2042 c         2638 g         3717 t
ORIGIN      
        1 taactgtgtt gttaaatcac gtgctttaaa tattgctttt tgtgctcgtc cggctacctt
       61 aataatacgt tcattttcgg gagaaaggtg ttcaataatt gaaatattac caaggattgc
      121 attgagcatg ttgttaaagt catgagcaat tcctcctgca aaaatagaaa gcgactcata
      181 ttttgctgtt tttatcattt catcttcata gcggacaaga tcggtgatat cacgtataac
      241 aacaatgcac cctgcaattt tattggcaat atagagagga ttgcaaacaa tataaacata
      301 ataggtatca ccattgttac gtatcatggt gttgcggtac aaagaaacag gcttcccctg
      361 tcgcagcacc tgagtaaccg ggtcttcata gggaattcct gtttttgcat tgtacaggat
      421 gcaaaaactg gcaaattgtt ttccaataac atcgtcctta aagccaaaaa gttttaatgc
      481 ggcatagttt atactggtga tagttccctt atcatcagtt gtgattattc cgtcagtaat
      541 tgaagaaaga atggtttgaa gctgtgcagt ttctttttga agattgctat tgcttatcaa
      601 aagctcctgc tgagtatttt caagctcttc atttattgcc gttaattcct cattcatagc
      661 ttctaattct tcggctgtag ccgacagctc ttcatactgc ttctgtatct tattttgagc
      721 tttatatgca ttgatgatac ccagcttatg aattgaaaag atttgatacg agatggcagt
      781 tacaaatgtt aatgctaatg cagaatccat aaaatattca ataacttccc atcgttcaag
      841 ccccaacgcg gcttgtacat acaatacaaa tataataagc aaaagcatat ttgcagtata
      901 gtaaaaccat atacctcgtc ttcgatcaac aaaaagaagc ggtgtatagg caaggcttgc
      961 agaaatcagc acaatggaat ccatacgttg cagcagagac ccggactcaa aaaatataac
     1021 aagccacaac gctatctgca cacttgcaaa tgagaggtta gccgcaaatt gaaattttcc
     1081 tttgcgaagc aatgctaatg aaataattgc agaaaaaatg gtacccgtaa aggggtaaag
     1141 gatgccgggg tcctttatgc cgcgaacagc aaaaaaggtt cccattatta ctacagcaaa
     1201 aataactgaa aaattaaaga tgagcgctgc ccacgctttc aggcgcaaca caatatcgtt
     1261 attttggtat ggttgtaaaa aatatctatc aggggaattc atgctggcac ctctcatttt
     1321 ttaacggcat gttctttgca aaacattaca actttgttca atcatgggat ggttgtctgt
     1381 tattgtttag tatttttaaa tgccgggcga tagttatcgg taaaaatttt ttcagtaaaa
     1441 tacaaaactt gtgttgactt aatttatgta tagtatatca ttgttgctat cgcactggca
     1501 gtgtgcggta gtattgcagt tgcacgctat ggattgtgaa aggaacaacc gtaacatcag
     1561 gatacatata tatgattgga gtattgtcat gtgcccatta ccagtaatca attgttcatc
     1621 gcgctcgcct atcgcaccgc tctatgcaac agccatcaag gcaggattcc catcgccggc
     1681 ggacgattat atagaaacgg tgctggattt aaacgaatat cttatacggc atcctgctgc
     1741 aacctttttt gtgcgtgtgt caggggattc catgacaccg gggatatgcc acggcgatat
     1801 ccttgtggtg gacagggctc tgaccccggt gcacaacaat attgtcattg ccgcagttaa
     1861 cggcgagctt accgtaaagc gtcttgtcat aaaaaacggc gccgccctgc ttgtgcccga
     1921 caatgacacc tatgaagcta tggctctgac ccctgagatg gattgtacca tatggggtgt
     1981 tgttattcat atcatccgga cggttggctg attatgtacg cgcttgttga ctgcaataac
     2041 ttctatgcct catgcgaaaa ggtgtgcaac cccgccctgt ggggtaaagc ggtggtggta
     2101 ctgtcaaata acgatggcaa tgtcatagcg cgaagccctg aggcaaaagc gcttggcatt
     2161 gcaatgggag cgccatttta ccagtgcact gatatcatcc gcaaacacca tgttgcagtg
     2221 ttttcgtcta actatgcgct gtatggcgac atgtctcggc gggtaatgga tacgcttgca
     2281 acctgtgcgc cgtatatgga ggtgtattcc attgacgagg cgtttttgct gtgcaatgtc
     2341 ccaccgtgcc atgcagaagg ctatgcgctg caggtaaagc atacggttct gcaatggaca
     2401 gggatgcctg tttcggtggg catgggcgcc accagaacgc tggcaaagct ggcaaaccat
     2461 attgcaaaaa aacgcacaca aagcggtgtg tttgtttttg tgggcgatag ttccctgtat
     2521 aaaaatttat tggtgcacat tgatgtacag gaggtatggg gcataggcgg gcgctacgca
     2581 cgtatgttac gcagacatgg catagacacg gtatggcagc ttatatgcgc tgatgacagt
     2641 tttataaaaa agaccatgac cattgtggga ctcagaaccg tgtatgaatt gcggggcatt
     2701 ccctgcatca gcacagagga tgcgccgccg ccaaaaaagt gcattgtaag ctcgcgctca
     2761 tttggcaaac cggtaacgga atgtaatgag ttgcaggagg cggcggcgca ctatgcatcc
     2821 atagcatcgc agaagttgcg gaagcaacat ggtgcagcgg gcgttgtcac ggtgtttgtg
     2881 tcaacaaacc cgtttaagga tgaaccacag tacagcagtg ctgccaccat tacgctggat
     2941 atgcactcgt gttatacgcc ggatatcatt gctgcggcac aaaccgcttt gcacaggctt
     3001 ttccggagcg gctatcgcta taaaaaggtt ggtatcatgc ttacggatat cgttgatgca
     3061 caggaactat cgcccacact tttttcacct gcgcaaagct gccggaaacg ctacaacctt
     3121 atgcaaacgc ttgacagcgt taatgccgca cgtggccgtg gcacggtgta ttttgcttca
     3181 gagggtgtgc agcggcaatg ggcaatgcgc cggcggtatc tgtccccgcg ctatacaacg
     3241 cactgggatg agataccgct tgtcaggtga taacggtatt gttgttgaca ataagcaaaa
     3301 atgtggcatt atttacccat gtcattgaga ctgcgataga tagtttattg taaagtttat
     3361 agttatgatg cactacgaac ctattcctga cacagtaatg tcatacattc ctgatataac
     3421 aaagatattg caggatgaag ctatcatttt tgcatatctt tttggtggat tagcacgtgg
     3481 tttttacaca cctctttcgg atattgatat tgctatttat attgatgacg taccggatgt
     3541 tgctgactat acattcaatc ttttttgcaa acttgcagaa gccgtaaaaa catctgagat
     3601 tgatattgtt atattaaata ctgcaccgga gagcattacg gggagaatag tattgaataa
     3661 agtaatctta acagataagg agccattaaa gcgatatata tatgaatcac tgacattgag
     3721 gaaatatttt gattttaaaa tgaaagagga gcagatttta ttcaggaggt ataacattgg
     3781 tagatatacc attaattctt aaaaagcttt cgaagctgga agaatattat aaacagatag
     3841 aagagtttaa aggtgttaca ttgaatgaat atagcaacga ctggaagata cagcgaataa
     3901 ttgaacggac attgcagata atgatagaaa cctgtgttga tatagcaaat catattattg
     3961 ctgataataa gttacgtatt ccggaaagct atgctgatac ttttaaggta ttacatgaaa
     4021 atactataag tatcagatga tttatttaat acaatgaaca aaatggtaaa attcagggat
     4081 gttgtggttc atcaatacag taatgttaat gccgaaatag ttatagcaat attaaaaaag
     4141 catagagaag attttatgaa ataccgtgac gcaattgtga tgtatttaaa aaatcatggg
     4201 tttaatgaat atcaccagaa taacgcataa agggatttgc tatgattgat ataaccgtca
     4261 taataatatt ggttgttatt gccacagttc ttttgtggac ggtggtgcta tttaaaattg
     4321 ttatgagccc gtttgcaatc agggacaaat ggaagctatt gggtgtgctg ctcatttttg
     4381 cgctatgtta tagtatcctg ccgtcgtggg caacaaaaac attgcacctc aaaagctata
     4441 ctataccctg tatcattgtg agcattcttt ttgttgggct tgtgatcctg tacacaatga
     4501 tcaatgcacg gttaaaaaaa tacaaatagg gttgcgatag atagtttata ggtaacatgg
     4561 ttttgccaat tccaaaacgc ggataatgcg attattggtt gacaggtatc caatacccgg
     4621 gtagaatacc atgataaatc agcaactcat aaagaaattt caaggaaacg atgattttag
     4681 aaagaaaata tgatattgaa gaaatacaaa aacgcatacg ctattaccct gtaacagcca
     4741 ttattggtcc acggcaatca ggtaaaacaa cattggcagg gatgttacga cctgatatga
     4801 tatttgatct ggaaaatcct gtggatatgg ctgcccttga aaatcctgtt ttacaacttg
     4861 ataaagaaaa gggattgata atcatagatg aaatacagct aaaaccagaa cttttcccgg
     4921 tattgcgata tctgtgcgat aaaaatcctg aatgcaggtt ttgtatatta ggaagtgctt
     4981 ctcctgatat aatcaaacaa tcatcggagt cattggcggg aagaatatca ttttatgtgc
     5041 tcatgggatt tcgtaaagaa gatttaccac acagtttgct ggagcatttt cacttcagag
     5101 gtggattgcc tcgttcattg ttagctccgg atgatactac aagtgttatg tggcgtaaag
     5161 attacgttaa aacattttta gaacgagata ttccattgct tggcatacgt attccgccct
     5221 ataccttgtt gcgattctgg cagatgctgg cacattatca tgcaaatgtt gttaattttt
     5281 cagagatagg caggtcatta gagatttcgg atacgcaggt caggcgttat attgagattt
     5341 tagcaggtac ctttatggtt cgcctattac agccgtggta tgccaacatt gggaagcgtt
     5401 tggtaaaacg tccaaaatta tatatcagag attccggtat actgcatagc cttcttatga
     5461 tagaaagcaa ggacgcgttg ttgcgccatc caaagttagg agcctcatgg gaaggatttg
     5521 cacttgaagc ggttgtgcgc tcaataaaaa agaatgatga tgaaattttc ttttatggtg
     5581 tccacagtgg tgctgaactg gatatggtat ggcaacatgg tggatatgca tggggttgtg
     5641 agtttaaatt ttctgatgca ccggtgctga caaaatcaat gcataatgca gtacaggatt
     5701 tgcagttaaa aaaggcatgg atagtatatc cgggtcaaaa aaaatataaa gtaaaaaata
     5761 ccattgaggt gataccattt gacgatatac cggatgtatg ggtatatgat taacaaataa
     5821 tgatataaaa aattttgcac tttgtaaagc aaagcaggta tgtctaaagt aactataatg
     5881 gaagatgaag taatgaatgg tgtttgccat gggcgatagt cactcttaat cgataatgcc
     5941 ggtggcgagg acagtcaaag caaagaatct tgttttgttg tattaaaaga atagattctt
     6001 tggggacggt gtttattaaa atgacaatgg tgggtatgtc atcctgagcc gaaggcgaag
     6061 aatctatttg ctaaaacgac atattcttgt gttgctttgc ttcttctgag tgacgatggc
     6121 aaatatttta ttgcaagggg gacttatgaa aaagcttatt gtagtgctgg ttatagcagg
     6181 tgtggttatt gcacagccac tgtttgcagc aaccattaca ggtactgcac cacaattacg
     6241 tgtatatgta ggtggatcat ggaacacaat tgatgtatca acagaattta atcaagccat
     6301 acttgatgcc aatgccgaat tggcaaagta tgatgatcag gataaactgg ctactggttt
     6361 tgccaatgcc aacatgtatt caaccaatgc cgccacacag cagggctatc ccaactatag
     6421 cctgtttgcc atcaccaccg gcgttatggt tggcgctcag gcacctacta ccaatgttga
     6481 ttattatacc aatggcaaga ttgaggatga tatagaaaac aaaggggatt tatatgctgg
     6541 tgttgcaggg agtgcagccg ttaatgtagg gataaacgca gggatgatta ttccggggct
     6601 ttatatcaat gtaaaatttg gtaagtttga cagtacctgg gtatatgatg atgacgactt
     6661 cagctgtgat accatgatta ttggtggcgg cattacctat agcctgtttg atgaagcagg
     6721 cactggctta ttgcgctggc gtggcctgac cattggcaca ggcttggtat atcaaacaac
     6781 cgatgtctca taccgggttg atttagactc aaagacagat tcctttacag tggaccctgg
     6841 tacaggtacc gacgttactg gcagtgtcgt tgttgaccct tcatttgaca ttacgcttga
     6901 tatgtataca ttaagcgttc cctttgagat caataccgct ttacaatttc tatggctctt
     6961 caacatcaat ctgggtgttg gcattgatct ggtaagtggt aatgccgatc tggagttaca
     7021 atcggtgtct gatgcgacgg ttaaaaacat tgcttatggt ggtactccgt tacctgaaaa
     7081 tgagtatatc gttactccgg gcaaggttac cattgacggt aaaacactgg ataaaaagcc
     7141 atcgtgggca cgggcgcgta ttatgacagg gattggttta aattttggag cggttaagat
     7201 tgacgtgccc attatctatt atttaaaaag tggtgcggct gttggggtga cagcggggat
     7261 aatatggtaa aatagggaaa aggggttttt gtcaaaaccc cttttatttt atagtggcgc
     7321 tgctgttgga atgacagcga tacgatgatg caatagagga aaaagttttg gcaaaatctc
     7381 atttgtttgt atagactgta ctggtgttga tgtaaaacag cgatatggta gtggtataca
     7441 agggaaaagc tttttagcaa attctttttg tttgtataac ggatactgct gttggggtaa
     7501 tgacagcgat atggtagtgg aataactttt ttaacagtgt atgaaatcta atttagtacg
     7561 aagtaatgac aacgatatgg tagtggaata acatgtcatt ctgaacgaag tgaagaatcg
     7621 tgttgttttg tgaaaggata gatttaacgg gactgtgttt ctcagaatga caatcataaa
     7681 gtttcaatgg ggaatgagaa tttttgtgga aaaaatagag agtggttccc ttataatata
     7741 accaaacgag ggaaaaaatc acgtttaagg tcaggcaaac gtacagtccg gaaaagtaag
     7801 aaatgatgat atttaaagag ttagcatatc acgatatagt ggtataatga ttttcaactt
     7861 gacagtgtat ttttttgatg ataggcagta ttttattaat cactatttaa tttcaatgag
     7921 gaaaagcaat gaagaaaata atgattttta ttatggttat cttgctatcc ggtgtttgtt
     7981 tcaatgtatc acattgcatt gctgaaagtg ctctggatca ggcaaaggat gctgcagaat
     8041 cgtctcaaaa ggcaaaagag gatgctgata agggtaaata tgaggaagca agagaaaaag
     8101 caggtggaca gtttgatgat gagtatgaca gagaaaaaga tcaagaatac ccaatacctg
     8161 agccggagat atcaaatgaa gatgattgat tagggaccat gtaatagttt tattcatttc
     8221 aggatttttt gtgatctctg gatacatata gagattctgg ttcaaccccg gaatggcgaa
     8281 ggtggtatgt cattctgaac gaagtgaaga atcgtgttgt tttgtgaaag gatagattta
     8341 acgggactgt gtttctcaga atgacggagg tggcaaaagg gattatgggt caagcccgga
     8401 ataactgtga gcaataaatc tatttttgtg gaaggagggc tcgtatatgc ggatatatac
     8461 aatgaaattg ataagctttt ttacatggtt atttattttt acaggctatt gctttagcca
     8521 ggaactatcg cccataacca tagtagcaac aggtaataac gcatcggggt ttgctctgtt
     8581 taagcagata gatggtaaac aaaaagataa aaatatcttt atctcgccat ttagcattgc
     8641 tatggcattt ggtatggcgt atgaaggggc aagcgggaat accgccagag agatagcttc
     8701 ggtttttgcg tttattcctg acgacaaaga caggtggaat gctttttcaa cgatatataa
     8761 aacacttaat cctgacaatg caaaatataa actggcaatt gccaacgctt tctgggttca
     8821 acaggattat cctttattaa agacgtacac tgacattttg aaaaattact atcactcaga
     8881 ggcttacaat gtagatttta cccatgacga taagcgaaaa gaggctgtaa aaaagatcaa
     8941 tgcgtgggca gcaaacaata cctatgataa aatacgcgaa gccataacat tggacgatgt
     9001 aaaaccgtta acaaggctta tacttaccaa cgctgtgtat tttaaagcta attggctcta
     9061 tcagtttgac aaaaatcaaa caaaagtaga taatttttat atcgatagta acaaaaaaat
     9121 aaaaacgccc atgatgatgc aacgcaatgt tacaattaaa tatatggaaa ataacaatat
     9181 ccagatgatt gaactgccct atgaggggaa ggctttgtct atgtttgtta ttttgcctaa
     9241 aaaggatatc aaaagtatta tgcctctgtt gaatgataaa aactttgaaa gctggcgtca
     9301 aaatttaata caggaagagg tggacgtgta tttgccaaaa tttaaggttg aatgcgggta
     9361 tatgcttgtt gagcctttaa taaagatggg cataaaggat gcatttaatg aaatgaaagc
     9421 cgatttttct aagattaccg gaggaaagga tttatatatt agcaaggtta ttcacaaaac
     9481 attctgtgat gtaaatgagg aaggaaccga ggcggctgca gttacggcta ttgttatgaa
     9541 tacaaaatcc atggtctctt ttgaaaagaa gatatttaaa gctgatcgtc catttatcta
     9601 tgctattgtg gacaataaca ccggagtaat attgtttttg ggtaaaatga taaaaccaga
     9661 gtataagtga tgatgtggat gaggagtcaa tttctgatta tgagtaaata gtattgaatg
     9721 ttttaggtta catgtgatgt ttaaccctgt ctatcttttg ttaacgtcta tcaggttcaa
     9781 gttaattttt ttacaataaa actagccaga aatgcttcca gcaaacttaa agggatggca
     9841 aaaggtagta ttgctattgg aaacacattc agattccagc ttacaagcca cattaatata
     9901 aatccagcaa accatgacag aaaagttgtt tgaatcaatg agaatttttt tgctataaaa
     9961 aatattgcga ttgcaaaaca caaagaccag ataccccata ctgcaccatt aattggttgg
    10021 gaagggaaca ccagaccaag tttattgtag tgctggatcc aataggattt tacaataaat
    10081 tcatttcgtg caaattcgga aacactaatc cagactgttg caataaaaat tgcgataatt
    10141 tgttttttta cggttttcat acaatactcc tgttgtttga atgggcattc cttgtatatc
    10201 attaaagggt gaatggatta atccaattgg tttgtatact atggttccca taatttactc
    10261 attttttatg aagacaattg aatgttagat gcaaaaatca cctcttcttt tttctgtgtg
    10321 aaatattttt ctttagcaaa tcgatattag cataatacgt attgttatca aaaagcaata
    10381 attctaaatc aattaattct ttaacatcgc gcattaaagt tctttttgac agccgattat
    10441 acattatagc tatggtaacg ttgagcgata ctatttcatc tagtgagagt ttttgtccta
    10501 ttggaatgtg caacattaaa tcacgtcgtc ttttattaag attttctgat tttcctctgg
    10561 tctgtttaat ctggaatgtc tcattctttt aacgaccatt ggcgtttgaa ggaaatgtat
    10621 ggataatttt tataaagttc ttccgtggaa taatctctaa catttagggt gcatattcac
    10681 tataatattt gtcatcagat atgtcaatgt aatattctgg aaaagtgact tcatcatata
    10741 tcaaaaatta cttgcattat ttaatgtttg agtgtttgtt ttttactaaa aatagggcat
    10801 ggtataattt ggcaccttgt agcaaaaaat attttaacta cagggaatag gcaaaattgc
    10861 atgtgaaatt atattaaacc tttattggga aaatattttt aaaggggaac aaaatgatga
    10921 agaaaattac tacaatcctg attgtggcgt ttttttctat ttattttatg ggcaatagtt
    10981 tgtatagcta ccagaaaaag tatattttta aagatcaaac tattatagat gagaaaaccg
    11041 gcataacaat aatgacaaat gaaacaaaag atcttattat tgttacaaat tatatacttc
    11101 aatatgcaat aatattccca tataacaatc aatgggaatt tgatatcaat gacaaatatg
    11161 ccattattgg caattgtgga acaataaata tttcactaca aattcatgaa aacaataata
    11221 aaacaactga agaatttctg tcagacctta tgaaaatata cactgaaaac aaatcaaaat
    11281 gtggagttat tgatgcaaag ctcatacaat ataataaaga tttagtacta cgaacggttg
    11341 ttgatccgac tatcatttat aaggatgata cctttaaaaa tgttaaacat atcaattact
    11401 actcaactaa gacattcaac aacattaggt atgtgtatca ttcttcggtt attgttgaca
    11461 atgataaaaa gattaatgaa gaggattttt taaattttgc aaccagagga ttctttattg
    11521 attttggacg aaaataaaac tttaaaatac tttaaaaagt ggtatgtagt ctaaatgccc
    11581 ctatccagat gcagatgagg ttattgcggg gtttggtgtc tataattgga aattaattga
    11641 gtatataatc acccatggtt gcattggcaa gatgataata aatactttat tatgttgcag
    11701 ttagacaaca tactacgcac caaaataata aacgtgcatg gtactggctc ttggcatgga
    11761 ttttcttgcc tgccaaaacc caacatcctc cagtaatgta ttaatcttga cagcgtaatc
    11821 tattatttgt aatgtaaaag caaatttttg aatagacata gtatatggaa aacgatctaa
    11881 aagcgttaaa acgtaaagct aaagcactgt tactatcaaa ggatattcct gcaggcaaaa
    11941 tggatattgt acgtgctttg cttgccaatg atcgagtatt agaagaagaa aagtacagag
    12001 caattattga tctcgttaaa aactatcctg ataagcctgt tatgccggta tcgcccggca
    12061 aagttgaagt taagggcgcg gatgaattta aaaaaacaat acaatatgat tcaaaaaata
    12121 caaatgtttt gtaccataaa tataaacata ccagactatt taaaaaacgc ctgctggtgc
    12181 atgcaaacaa caagtttggt ataggctttt tacggcggct tattccatcg caaaaatttt
    12241 taaaagtaag ccagcggtta tttgatctgc aatctgcact gcgccagcgt ctgtacaaaa
//
LOCUS       DAOSOX010000061        12296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5143_length_12296_cov_9.177682, whole genome shotgun sequence.
ACCESSION   DAOSOX010000061 DAOSOX010000000
VERSION     DAOSOX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12296
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5143_length_12296_cov_9.177682"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLX22_06075"
     CDS             complement(<1..792)
                     /locus_tag="PLX22_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19500.1"
                     /translation="MQAIVTIENLTIAFTSMYGKNYAVRDLSLQIPRAKTLGLVGESG
                     CGKSVTAYSLLQLVPKPGVIEKGAIMYNTLNLLQCPDATLQSIRGKNISMIFQEPMTS
                     LNPVFTIGYQVQEAVELHLHLQKAEAKDYTIALLGKAGIPSPSSRYYSYPHELSGGLR
                     QRAMIAMALAAKPELLIADEPTTALDVTIQSQILDLLLTLQKSQHMSMLLITHDLGIV
                     ANVADVIAIMYAGEIVEIAPTREIFNNFLHPYTQGLMSCIPSLHKK"
     gene            complement(792..1073)
                     /locus_tag="PLX22_06080"
     CDS             complement(792..1073)
                     /locus_tag="PLX22_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19501.1"
                     /translation="MQREILFDSIHEFINTLRTNDITKVAFTQINEKRAREVAPGTLQ
                     VVDVATAEILAYKDATIYKYKRDNADLPQLYAMLQSQGFELIKRSRNIV"
     gene            complement(1088..1825)
                     /locus_tag="PLX22_06085"
     CDS             complement(1088..1825)
                     /locus_tag="PLX22_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19502.1"
                     /translation="MQKNITTQPIRFVIINNTSPGSPYAAYDAQLATIIQSINNENPV
                     FTIHSGNIVHGGTIAMGIHEKDIETQYKQFYRYCSKLNSILFTACGPQDTYNNSTRLY
                     TRFTQKQAWYSFNYGSHHFIVLYSEDNNVLKLPQQELQWLHDDVKQNQHASKVVFCYF
                     LPAIHKNNSQTFIHNDYQHLHTMLKQYNVIAFIGVTGISSPVTIDTIQYIPVPCDNTF
                     TNKYKKAIRYYIVDFNGNYIKLVPRYL"
     gene            2625..4526
                     /locus_tag="PLX22_06090"
     CDS             2625..4526
                     /locus_tag="PLX22_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT19503.1"
                     /translation="MADRWYITQQTFPELLNRNVIKYGTRRAQWWKRGDTTHSINYAE
                     LGFIVKQLTAGLMSLGIQKGDRVAIASHTAPQWMWADYSILCAGGITVCIYPTLSAKE
                     MSFIINDSGSKILYVQDEEILQRALSVADEMPTLQKCIIMKDDYKGSDSRVLTLPQVR
                     ELGIQLLAKDKLAYESRWRSVDILDPMTIVYTSGTTGRQKGVVHTHFSINAACCRDLR
                     IVPEYREDDIMLSFLPLAHTYERECGHGTAMMAAVTIAYSSPMTLIEDLKVFKPTVFM
                     SVPRIYERVFMAMRDMASKSPVTKKIFEFAIKTGLQLTEARSDENGFVDMSEGINFTE
                     GVGKWLAFKYKLVDKIIFSKVRETLGGRFRFAFSAAGSLSADLCKVFMAMGVRIYEGY
                     GATETCNTINLNLPHKVLPGYVGPVCNGVEGRIASDGEWQVRGNNIFTHYWNNPEATN
                     ETFTEDGFYKTGDIVQMGPDGYIKIVDRKKGIMVLDTGKNVPSAKIESLFSVSRYVDI
                     VFPIADDKPYVGALVVPKYDVFIELFKKEGIPYDQSKLVFFEEGATRTCVAVGDDFIQ
                     NELLRKLVDEDIQQANKELEEYERIKKYVIVNRKFSESNGEMTPTLKVKRNVVLKNFE
                     KEIQELYKK"
     gene            complement(4597..5454)
                     /gene="argB"
                     /locus_tag="PLX22_06095"
     CDS             complement(4597..5454)
                     /gene="argB"
                     /locus_tag="PLX22_06095"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOT19504.1"
                     /translation="MKQYIEKVNTLIESFPYIKEFYGKTIVIKYGGHAMETLEGKRSF
                     ALDMVLLKYVGINPVIVHGGGPQINTLLEQVGVQSAFVGGMRVTDASTMEIVEMVLVG
                     KVNKEIVNCINLAGGRAVGLSGKDGNLLIAEKIYHTANGEKKDIGQVGTITKVNPLII
                     ETLDREKFIPVIAPIGCDNNGITYNINADIAAGKLAEALKAEKLILLTDVEGVKIDGK
                     LISTLRKDEVDNLIISNKISGGMIPKVNCCLGALKEGVGKTHIIDGRVEHAVLLEIFT
                     DAGIGTEIV"
     gene            complement(5451..6830)
                     /locus_tag="PLX22_06100"
     CDS             complement(5451..6830)
                     /locus_tag="PLX22_06100"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOT19505.1"
                     /translation="MKIHYPGYETVINNVYDSGNEHVFAYWDTLNPDQKKHLLEELST
                     IHFETLQQLFELTKRNLPPLDFKPAPYTPLPETDEEKKEFEKAKQIGIATIKAGKVAA
                     FVVAGGQGSRLGFEGPKGMFPIGPVSKKSLFQIHAEKIRAYEIKYNVTIPWCIMTSEA
                     NHDATVAYFEQSNFFNLNKEKVFLFKQNMIPSLDENGKLILESPCSIFRNPDGHGGSL
                     TALYQSGTLDTLQALTIEILSYFQVDNPLVNIIDPVFIGFHRMHSAEVSSKALVKAYP
                     EEKIGVFVQFSNNRIGVVEYSDLPPEKQYEKDEQGQLRYAAGSIAIHLFNTQFIASVT
                     GSNVTLPFHVAKKSIPKYTDKGIQHITGYKFEKFVFDALPLTQHNFILETKREEEFAP
                     VKNKTGVDSVESAQELMIHLHKRWLTQKGIAIPTMLKKIELSPLFAVEKDDIPQSLII
                     PDKEEVYIE"
     gene            complement(7216..7905)
                     /locus_tag="PLX22_06105"
     CDS             complement(7216..7905)
                     /locus_tag="PLX22_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19506.1"
                     /translation="MKYVYCIGIVIISMCMVLPVYADDLGIGISAWYADWEMDNSSNP
                     KKTMDPVLCIGPSLSYQFSETLSVTLVALFTPKKYEMPDEGSEDSKLRRYDSDLALNY
                     QLNRYIKLFAGAKYLGFTFENGKHHGAGPGMGIGFTIPIVYNFYFLGNASGLYLYGNH
                     NDNSNDTNYNEYGYNLAGQLAYYFPSIGLTTSLGYRYQYIKSDYNTNNMADDEHTFKG
                     FTCLVVKSFHL"
     gene            complement(8269..9456)
                     /locus_tag="PLX22_06110"
     CDS             complement(8269..9456)
                     /locus_tag="PLX22_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT19507.1"
                     /translation="MKLHVVTSNNVDNLLSDNVITLPPSGIREFFDIVYNTPDCISLG
                     VGEPDFVTPWRISDAGIYAIKDGNTHYTSNKGLQELRQHIAAIISKKHNVLYDPETQI
                     LVTNGVSEGLDIALRSIINPGDEVIYFEPCYVSYSATIKLAYGKAVAIPTHFDNGFAI
                     DIDQLQKTITHKTKAIMLNYPANPTGASIDKQTLEAIASIAIHHNLIVIADEIYDEIT
                     YNGNHFSIISIPGMPERTIYLNGFSKAYAMTGWRLGYACGSESIISAMNKIHQYTALC
                     APSIAQYAAIEAITHGQRDVATMRSHYVKRRNFISSRLQEIGLPCYPPRGAFYVFPSI
                     AKTGLSSREFAVRLLKSHKVAVVPGTAFGSWYDNHIRCSYATSIEQLKEAIDRIEDFV
                     NRL"
     gene            complement(9522..9743)
                     /locus_tag="PLX22_06115"
     CDS             complement(9522..9743)
                     /locus_tag="PLX22_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19508.1"
                     /translation="MEIVSYILNNTTMFVIIAGLLIACAGFLLLRLRIVSIVCIILAA
                     LIIYVLLYKTSPIPTQPTETREIDRIDKL"
     gene            complement(9836..10786)
                     /gene="glpX"
                     /locus_tag="PLX22_06120"
     CDS             complement(9836..10786)
                     /gene="glpX"
                     /locus_tag="PLX22_06120"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOT19509.1"
                     /translation="MDRNLALEVVRVTEAAALWAARYYGRGNEDLAGKNAVEAMSKVF
                     STVSFKGHIICGIDNPDSSLCTGKIIGNAQHPEVDIALTPIDGHATLAQGGYNTISVI
                     ALAEYGSMLNAPPIYMEKIAVGKDARGVIDITQSPEVNIKRVARAKGKYIDDITVCIL
                     DRARHEELVSRIRALGARIKLIKDGDISGAVATALDDSPIDMLMGVGGAREGILSAAA
                     LKCLDGDMQARFIYRDEKDKELVRNMGITDIDKIYTLSDLAKGENIMFSATGISDGEL
                     LPGVRFMAGGAKTHSIVMRYKTHTIRYITAIHRFDYKPVY"
     assembly_gap    10946..11045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11046..12080)
                     /locus_tag="PLX22_06125"
     CDS             complement(<11046..12080)
                     /locus_tag="PLX22_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19510.1"
                     /translation="MHYIFSAIAILCLTTTAFAKGIPLDAVYMDKNSSEYQKLFFARL
                     NNYAQMGAQFIDSNIVYSAWCSGKEIVYIKESGINNTIVVCAITTRKKNPLYTFKGTV
                     ISARTSSNGRYIAVKYFTNQRIPQPALFIIDCIGGTVKLLQTLSAGNDFSFSDDGNIF
                     YYKKNNCIVAYRCDSSMEKAFLCSSDAPQWDEICTIIFNSNQLFVSGSSGNYTVYIKQ
                     KLWKKICNAVTPAEVFSDSFAIYYTGGYPGNYTVHRYSVKTQKHETLMQQSLNPCLQV
                     SRNHAIFLKNAFIMMYDSINGRLFTTTVESDEAIISPNGFYVASTIYNRLFIIPYATV
                     VTINPKAGRYL"
     gene            complement(12084..>12296)
                     /locus_tag="PLX22_06130"
     CDS             complement(12084..>12296)
                     /locus_tag="PLX22_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT19511.1"
                     /translation="DCLWRIAMKVYNNARLWPLIYIANKDQIKDPDLIFPGQRFVIPP
                     MEKEKQPIEESQSKTMDDKKPQDVKE"
BASE COUNT         3655 a         2266 c         2387 g         3888 t
ORIGIN      
        1 ttttttgtgc agtgaaggta tacagctcat aagcccctgt gtataggggt gtaaaaaatt
       61 attaaatatt tctctggttg gtgctatctc tacaatttct ccggcataca ttatggcaat
      121 gacatcggca acgtttgcaa ctatccctaa atcatgtgtg atgagcaaca tggacatatg
      181 ctggcttttt tgcagtgtca gcagcaggtc aagtatctgt gactgaatgg taacatcaag
      241 cgctgttgtt ggttcatcgg caattaaaag ttcaggcttt gccgcaagcg ccatggcaat
      301 cattgcacgc tgtcgcaaac cacctgatag ctcgtgagga tacgagtaat atcggctgga
      361 agggctggga attcctgctt ttcccagtaa tgcaatggtg taatcttttg cttcagcttt
      421 ctgtaaatgt aaatggagct caaccgcttc ctggacctga tatccaatag taaatacagg
      481 attaagcgat gtcattggtt cctgaaatat cattgaaatg tttttgccgc gtatggactg
      541 caatgttgca tccgggcatt gcaaaaggtt cagggtattg tacatgattg cacctttttc
      601 aatgacaccg ggctttggaa ctaactgcaa caatgaatat gcagttaccg attttccgca
      661 tcctgattcg ccaacaagcc ctaatgtttt tgctctgggt atttgcaggg agagatcacg
      721 aacggcatag ttttttccgt acatgctggt aaatgcaatg gtaaggtttt ctatagtaac
      781 tatcgcttgc attatactat atttctgctt cgttttatta attcaaatcc ctgactctga
      841 agcatggcat acagttgcgg taaatcagcg ttgtctcttt tatatttata gatagttgca
      901 tctttatagg caagaatttc tgctgttgca acatcaacca cctgtaacgt gccgggtgca
      961 acctctcgtg cgcgcttttc attgatttgc gtaaaggcaa cttttgtaat atcgttggta
     1021 cgtaatgtgt ttataaactc gtggatggaa tcaaacaata tttcgcgctg cataacccac
     1081 cttactgtta caaatatcgt ggaacaagtt taatgtagtt gccgttaaag tcaacgatat
     1141 aatagcgtat agctttttta tatttattgg taaaagtatt atcgcacgga acaggaatat
     1201 attgaatggt gtcgatagtt accggtgatg aaattcctgt gacaccaata aaggcaataa
     1261 cattgtactg tttcagcatg gtgtgtaaat gctgataatc attatgtatg aatgtttgtg
     1321 agttgttttt atgtatggct ggcagaaaat agcaaaaaac aactttactg gcatgctgat
     1381 tttgttttac atcatcatga agccactgaa gctcctgttg aggaagttta agcacattgt
     1441 tgtcctcact gtacagtaca ataaagtgat ggctgccata gttaaatgaa taccatgcct
     1501 gcttttgtgt aaagcgtgtg tacaaccggg tggagttatt gtaagtatcc tggggtccac
     1561 aggcggtaaa aagtatactg ttgagtttac tgcaatagcg gtaaaactgt ttatattgtg
     1621 tttctatatc tttttcatgt attcccatag cgatggtacc gccgtgtaca atgtttccgg
     1681 agtgtatggt aaataccgga ttttcgttat tgatggattg aatgatggta gcaagttgtg
     1741 catcataggc tgcatagggt gaaccgggcg aagtattgtt aatgatgaca aaccgtatag
     1801 gttgcgtggt tatatttttt tgcacactgc atgctgttac taataaaagc aacaatgtac
     1861 ataagcaatt taatgggtat agtaatcgtt tcatgcttac cattgttggt taccatatat
     1921 tttttacaag tttttattgg tattgccatt aaaaatgtat taacaatata ctcgataggg
     1981 cacacaatcg agttgctgtt gccatgcgat aagggtacat cgtcatgcaa atcgaggcaa
     2041 ccagtataaa aaatcgcatg gcaccatacc aggtataaaa gaatgtatta acggtaatga
     2101 atttttttac atcctgagtt ctcttgacaa gtttaaaaac aatgtgatta tacgacctta
     2161 tttttacacg ccttgagctt taacaccagg gacaatccca tagtggtgat tctatcgtgt
     2221 caatgaatta ttttatgttt acaaatcccg ttagacttga cgttgttata tgagcaggat
     2281 tatattataa gaaatttttg ctagtattaa tacaatggtt aatatcatta atcagtgagt
     2341 tatataacgc tgtatgaatg ctaaaatggg taggtactgt ctatcttttg atgccaacaa
     2401 ttgttatcgg gtatagagca ttgtaatggc taaatactga taaatgtgca aaatttgtcc
     2461 cacgttatat attgattaga catttaatag cattgcctgc agactaaatt tttacataaa
     2521 aatttaaaaa aatgatatat aacttgacat atacaaatta gaataccatt gtattataag
     2581 gtattaccaa tagtgtttac aaacaatttt tataggaggt atttatggct gatagatggt
     2641 atataacaca acaaacattt cccgagttgc ttaacaggaa tgtaataaaa tatggcacca
     2701 gaagggcaca atggtggaaa agaggggata ctacacattc aataaactat gctgagcttg
     2761 gatttattgt taaacagtta accgccggac tcatgagttt aggaatacag aaaggtgatc
     2821 gcgttgctat agcatcgcat actgcacctc agtggatgtg ggcagattac tcaatactat
     2881 gtgctggcgg tatcactgta tgtatctatc caacgctttc tgcaaaagag atgagcttta
     2941 tcatcaatga ttctggatca aaaattttat atgtacagga tgaagaaatt cttcaaagag
     3001 ccctgagtgt agctgatgaa atgccaacac tgcaaaaatg tattattatg aaagatgatt
     3061 ataagggttc tgactctcgt gttcttactc tgcctcaggt aagagaattg ggtatacagc
     3121 tattagcaaa agataaatta gcgtatgaaa gtagatggcg ttcagttgat atacttgatc
     3181 ccatgactat tgtgtataca tcgggaacta ctgggaggca gaaaggggta gtccatactc
     3241 atttcagcat taatgcagca tgttgccggg atttgcgtat tgtcccggaa tatcgagagg
     3301 atgatattat gctttcattt ttgcctcttg cacacaccta tgaacgtgaa tgtggtcatg
     3361 gcactgccat gatggctgcg gttaccattg catactcttc accaatgaca ttgattgaag
     3421 atttgaaggt gtttaaaccc acggttttta tgtctgtacc aagaatatat gaacgtgttt
     3481 ttatggctat gcgcgatatg gcatctaaat ctcctgttac taaaaaaatc tttgagtttg
     3541 caataaaaac tggtttgcaa cttactgaag caaggtctga cgaaaatgga tttgtagata
     3601 tgtccgaagg tattaatttt actgaaggcg taggcaaatg gctggcattc aaatataaac
     3661 tggtggataa aattattttt agcaaagtta gagaaacact gggtggacgt ttcaggtttg
     3721 ctttttctgc tgccgggagt ttatctgctg atttatgcaa agtttttatg gcaatgggag
     3781 ttagaattta tgaaggatat ggagctactg aaacatgtaa taccatcaat cttaatctgc
     3841 cccataaggt tttgcccggt tatgttgggc ctgtatgtaa tggcgtagaa ggaagaatag
     3901 caagcgatgg tgaatggcag gtacggggta acaatatatt tactcattac tggaataatc
     3961 cagaagcaac aaatgaaact tttactgaag atggatttta taaaacaggt gatatcgtac
     4021 agatgggacc tgatggatat atcaaaattg tagacagaaa gaaaggtatt atggtccttg
     4081 ataccgggaa aaatgttcct tcggcaaaga ttgaaagctt attttcagta tctcgatatg
     4141 tggatattgt attccctata gctgacgata aaccgtatgt tggcgcactt gttgttccaa
     4201 aatatgacgt tttcattgaa ctgtttaaaa aggaaggtat cccatacgac caatctaaat
     4261 tggtgttttt tgaagagggt gcaacacgca catgtgttgc cgttggagat gattttattc
     4321 agaatgaact gttgcgcaaa ctggtagatg aagacataca gcaagccaat aaagaattgg
     4381 aagaatatga aagaattaaa aaatatgtaa tagtgaacag aaaatttagt gaatcaaacg
     4441 gggagatgac cccaacactt aaggttaagc gcaatgtagt tctgaaaaat tttgaaaaag
     4501 agatacagga gctttataaa aagtaaattt attgtttatt ataccatagt actgatatat
     4561 aggcagctaa tgggatgttt taaggctgcc tatatatcac actatttcag tgccaatacc
     4621 tgcatcagta aagatttcca gtaatactgc atgttccacc ctaccatcaa tgatatgcgt
     4681 tttgccaacg ccttctttga gcgcaccaag acaacaatta actttgggta tcatacctcc
     4741 gctaattttg ttactgataa tgagattatc aacttcatcc ttacgcaatg ttgaaataag
     4801 tttgccatca atttttacac cttcgacatc tgttaaaagg attaattttt ctgcctttaa
     4861 agcctctgcc agcttgccag cagctatgtc agcattaatg ttataggtta taccattatt
     4921 atcacagcca ataggggcta tgacggggat aaatttttcc ctgtccaatg tttcaatgat
     4981 aagaggattt acttttgtta ttgttccaac ctgcccaata tccttctttt caccattggc
     5041 agtgtgataa attttttcag caataaggag attgccatct tttccggata aaccaacagc
     5101 tctgccacca gccagattaa tacaattaac tatctcttta ttaacttttc caactaaaac
     5161 catttcaaca atttccatgg ttgaagcatc ggtaacacgc atacctccta caaacgcgct
     5221 ctgtactcct acctgctcca acagtgtgtt aatctgaggt ccgccgccat gaactataac
     5281 aggattaatg ccaacatatt tcaaaagtac catgtccagc gcaaatgaac gcttaccttc
     5341 aagggtttcc atggcatgac caccatattt tataacaatg gttttaccat aaaactcttt
     5401 aatatagggg aatgactcaa taagcgtatt aactttttca atgtattgtt tcattctata
     5461 taaacctctt ccttatcagg gattatcaaa gattgtggga tatcgtcttt ttcaacagca
     5521 aacagtggcg atagttctat ttttttaagc atagtgggga tagcaatacc tttttgtgtt
     5581 aaccatcgtt tatgtaaatg tatcataagt tcctgtgccg attcaactga atcaacgcct
     5641 gttttatttt taaccggtgc aaactcttcc tcacgttttg tctccaggat gaaattatgc
     5701 tgtgttaacg gaagtgcatc gaagacaaat ttttcaaact tataaccggt aatgtgttga
     5761 atacctttat cagtatattt gggtattgat ttttttgcta catggaatgg cagcgttaca
     5821 ttgctgccag taacagatgc aatgaactgt gtattaaaaa gatgtattgc aatgcttcct
     5881 gctgcataac gcagttgtcc ctgctcatct ttttcatatt gcttctccgg cggcaggtcc
     5941 gaatattcta caactccaat cctgttattt gaaaattgta caaacactcc aatcttttct
     6001 tcaggatatg cctttaccag agctttactt gaaacttcag cactatgcat tctatggaat
     6061 ccaataaata caggatcaat aatgttaacc agtgggttgt ccacctgaaa gtatgaaagt
     6121 atttctatgg ttaatgcctg caatgtatca agtgtgcccg actgatataa cgctgttaaa
     6181 cttccaccat gcccgtcagg atttctaaaa atagagcagg gcgattccag tataagcttt
     6241 ccattttcat ccagtgaagg aatcatgttt tgcttaaaga ggaagacttt ttctttgttg
     6301 agattaaaaa aattgctttg ttcaaaatat gctactgttg catcatgatt ggcttctgag
     6361 gtcatgatgc accatggtat ggtaacatta tactttattt catacgccct tattttttct
     6421 gcatggatct gaaacaatga ttttttgctt acaggtccta ttggaaacat cccctttggg
     6481 ccttcaaatc ccaagcggct tccctgccca ccagctacca caaatgcagc caccttgcct
     6541 gccttaatgg ttgcaatccc aatttgtttt gctttttcaa attctttttt ttcttcatcg
     6601 gtttctggca aaggcgtata cggcgctggt ttaaaatcca atggaggcag gtttcttttt
     6661 gtaagttcaa atagttgttg cagggtttca aaatgtatgg tcgatagttc ctctaaaagg
     6721 tgttttttct gatcaggatt taatgtatcc cagtaagcaa atacatgttc attcccggaa
     6781 tcatatacat tgttaataac ggtttcatag ccaggatagt gtatcttcat agagttcccc
     6841 ttggtcaatg caatgctttc ctataatctg gagcatggag taaatttgta tgcatacagt
     6901 gaaatgcttt ttaacaacta ttctgcatat catgaattat cctgcaagat attattaaaa
     6961 taaaaccaat caggataaaa accagtgtat cacaaaaatt attaccaatc aaatgattaa
     7021 ggatatcggc tcatatccag aaacacatgc tgctgcgttt tgggtgcatc acaattcgcc
     7081 cctgcataat gtcatcataa tgaatacaaa gatatattcc atcacaattt aaaaacgact
     7141 atttttttat ttcaacagaa aattaataaa ggcagtataa aaataaaatg atatgtctac
     7201 aaacaatgtc tgcttttata aatgaaatga tttaaccacc aggcaggtaa atcccttaaa
     7261 agtatgctcg tcatcagcca tattattggt attatagtca gatttgatat attgatagcg
     7321 gtatcctaat gatgttgtta aaccaatgga tggaaagtag taggctaact gcccggcaag
     7381 attataacca tattcattat aatttgtatc attagaatta tcgttgtgat tgccatagag
     7441 atataaaccg gatgcattgc ctaaaaaata gaaattatag acgatgggga tggtaaagcc
     7501 aatacccatg cccggacctg ctccatggtg cttaccgttt tcaaaggtaa atcctaaata
     7561 tttagctcca gcaaatagtt taatatagcg gtttaattga taattgagcg ctaaatcaga
     7621 atcgtaacgc ctgagttttg aatcttctga cccttcatca ggcatttcat actttttggg
     7681 agtaaagagg gctacaagag ttacacttaa agtttctgaa aattgatacg aaagtgaagg
     7741 gccaatacat aaaacagggt ccatggtctt tttgggattg ctactgttgt ccatctccca
     7801 atcggcatac catgctgaaa tgccaatacc taaatcatca gcatatactg gcaaaaccat
     7861 acacatgctt attatcacta taccaataca gtatacatac ttcatacatt atcctccgaa
     7921 aatggaatta ttatcctcac tctctgctcc acctccttta taaggcgagg ggagttttcg
     7981 caccattgtc attatgaggg agcgttagtg accgaagaat ctatgctttt aacgacgaga
     8041 ttcttcactt tgttcagaat gacatgtcac agcaagattc tttgcctaac gctcaggata
     8101 acaggtgcta tcgcttttga ataacagttt gctgatttct cttacctatc atcatgctaa
     8161 acgtatttca gcagcaattg tttgcaattg cactatgtag aggagataat ggcaagaatt
     8221 ttttaattat atgataacta tcgctaaaaa ataaggattt ttgatcgttt acaatctatt
     8281 cacaaaatcc tcgatcctgt ctattgcctc cttcagttgc tcaatagatg tggcgtatga
     8341 acatcgtatg tgattgtcat accatgaccc aaacgctgtg ccgggaacta ctgcaacctt
     8401 gtggcttttt aaaagtcgta cagcaaattc acgcgaggat aaccctgtct ttgctatgct
     8461 gggaaaaaca taaaatgcac ctcggggagg ataacatggc agtccgattt cttgtaaccg
     8521 tgatgatata aaattacgcc gtttaacata gtgcgaacgc attgttgcaa cgtcacgctg
     8581 tccatgggta attgcttcaa tagcagcata ctgtgcaatt gatggagcgc acaatgcagt
     8641 atactgatgg attttattca ttgcagaaat aatactttcc gagccacatg cataccccag
     8701 tcgccagcct gtcatggcat aggcttttga aaatccattg aggtaaatgg tgcgctcagg
     8761 catgcccggt attgaaataa ttgaaaaatg atttccatta taggtaattt catcataaat
     8821 ctcgtcagca atgacaatga gattatggtg tatggcaatt gaagctattg cttccaatgt
     8881 ttgtttgtca atggaagcac cagttgggtt agcaggataa ttaagcatga tagcttttgt
     8941 tttatgggtg atagtttttt gtaattggtc aatatcaata gcaaatccgt tgtcaaaatg
     9001 agttggtatt gcaacagctt tcccatacgc taatttgatg gttgcagaat aggaaacata
     9061 gcatggctca aagtagatga cctcatcacc gggattgatg atactgcgca aagcgatatc
     9121 aagcccttcg ctgacaccat tagttacaag aatctgagtt tcgggatcat atagtacatt
     9181 gtgttttttg ctgatgatag ctgctatgtg ttgccgcagc tcttgaagtc ccttatttga
     9241 ggtatagtga gtattgccat ctttaatagc ataaatacct gcatcagata tgcgccatgg
     9301 agtaacaaag tcgggttcgc caactcccag tgatatacag tcaggggtat tatatacaat
     9361 gtcaaaaaat tcacggatac ctgatggcgg cagggttata acattgtccg ataaaagatt
     9421 atcaacattg tttgatgtaa caacatgcag cttcatagtc ttctccaatc gtaagtacaa
     9481 aatataatat acaatattgt attcatttta acaataaaat tttatagttt atcaatcctg
     9541 tcaatctccc ttgtttcagt gggttgtgtc gggattggtg atgttttgta cagcagtaca
     9601 taaataatca aagccgccag tattatgcaa actatcgaca caattcgtag ccttagtaac
     9661 aaaaatcctg cgcatgcaat taatagacca gcaatgataa caaacattgt agtgttgtta
     9721 agaatatatg atacaatttc cataactcta ctcctgcatg gttgccatgt tcatacagtt
     9781 atgcctgtca ttgttaagaa aaggtgctgc atcactgtat gaataactca ataaatcaat
     9841 ataccggttt gtaatcaaag cggtgtattg cggtgatata tcgtatggta tgtgttttat
     9901 agcgcattac aatagaatga gtttttgctc cccctgccat aaatctgacc ccgggcaaca
     9961 gttcaccatc tgagattccc gttgccgaga acataatatt ttcgccttta gccagatctg
    10021 agagggtata aattttatca atatcggtaa tccccatgtt gcgcaccagt tctttatcct
    10081 tttcgtcacg gtaaataaaa cgcgcctgca tatcgccgtc cagacattta agcgctgctg
    10141 ccgataaaat accttcacgg gcaccgccaa ctcccatgag catatcaatg ggactgtcat
    10201 caagtgcagt agcaacagca ccggaaatat caccatcctt aattaatttg atacgagcgc
    10261 ctaatgcccg tatacggcta accaattctt cgtgacgtgc tctgtcaaga atgcagaccg
    10321 ttatatcgtc gatatatttt cccttggcgc gtgcaacacg tttaatatta acctctggtg
    10381 attgtgttat atcaataaca cctcgtgcat ctttaccaac tgctatcttt tccatatata
    10441 tgggcggggc attgagcatt gagccatatt cagcaagtgc aataacggag atggtgttat
    10501 agccaccctg agctaaggtt gcatgcccat ctattggcgt aagagcgatg tcaacttcgg
    10561 gatgctgagc attaccaatt attttgccag tacaaagtga tgaatcagga ttatcaatgc
    10621 cacagataat atggccctta aatgaaaccg ttgaaaaaac ctttgacatt gcttccacag
    10681 catttttgcc agcaagatct tcattgccac ggccatagta acgcgctgcc cagagtgctg
    10741 cagcttcggt aaccctgaca acctcaagtg ctaaattgcg atccatacaa cttcccttaa
    10801 taattctaat ttttccttct gagtatggct acccaatgga ttgcttttgc agggagtatg
    10861 aaaatccctc cagcttccaa ataaaacagt ttaacacata ctattgttat aaaaattgtc
    10921 aaattaatat tttttaaaaa tgacannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnaaggt atcgccccgc ctttggatta atggtaacaa cagttgcata ggggataata
    11101 aataatcggt tgtagatagt tgatgcaaca taaaatccgt tgggtgaaat aattgcctca
    11161 tcactttcca ctgttgttgt aaacagtctc ccgttaatac tgtcgtacat cataataaat
    11221 gcatttttaa gaaagattgc atgatttcgg ctgacctgta aacaggggtt aagtgattgc
    11281 tgcatgagcg tttcatgctt ttgcgttttt actgaatagc gatgtaccgt ataatttccg
    11341 ggatatccgc ctgtataata gatggcaaaa ctatcgctga agacctccgc cggggtaaca
    11401 gcattgcaaa tcttcttcca tagcttttgt tttatataga cagtgtagtt gcctgatgaa
    11461 ccgcttacaa acagttgatt gctgttaaaa atgatagtac agatttcatc ccactgcggt
    11521 gcatcgctgc tgcaaagaaa agctttttcc atggaactat cgcaccggta tgcaacaatg
    11581 cagttgttct ttttgtaata aaaaatgttg ccgtcatctg aaaatgaaaa gtcgttgcct
    11641 gctgataagg tttgtagcag tttgacggta ccgccgatac aatcaatgat gaaaagtgca
    11701 ggttgaggta tcctttgatt ggtaaagtat ttaacggcta tgtagcggcc gttggatgat
    11761 gttcgcgctg agataaccgt tcctttgaat gtgtacagtg gatttttttt acgtgttgtt
    11821 atggcgcata caacaatagt gttgtttata cctgattcct taatataaac aatttcttta
    11881 ccgctgcacc atgcactgta aacaatatta gagtcaataa attgagcacc catctgggca
    11941 tagttgttaa gtcgtgcaaa aaaaagcttt tgatattcag atgaattttt atccatatat
    12001 acagcatcaa ggggaatgcc ctttgcaaaa gctgtggtcg ttaaacaaag tatggcaata
    12061 gcgctaaaga tatagtgcat tgattattct tttacatcct gaggtttttt atcatccatt
    12121 gttttagatt gtgattcctc tatcggttgt ttttccttct ccatgggtgg aataacaaac
    12181 cgctggccgg gaaatatcaa atcaggatct tttatctgat ctttgtttgc aatataaatg
    12241 agcggccata accgtgcatt gttatatact ttcatagcaa tccgccataa acaatc
//
LOCUS       DAOSOX010000062        12162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5210_length_12162_cov_6.902784, whole genome shotgun sequence.
ACCESSION   DAOSOX010000062 DAOSOX010000000
VERSION     DAOSOX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5210_length_12162_cov_6.902784"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLX22_06135"
     CDS             complement(<1..910)
                     /locus_tag="PLX22_06135"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOT19512.1"
                     /translation="MEKHLIQLVTITLLFFSPCSVFAQENTSTTNADASWVPFTEGNW
                     PTTPTLAMFIAVPVAITIYGTVTWDWGSQKNPHFGDEGFFGKNTYSGGADKLGHAFSH
                     YVMFRALYNYYDWSENGKSTRWFYSITTASFVGILIEVGDAFTGEYGFSYEDVVSDMT
                     GIGLGILLEYSPTLDSLIGFSWQYWPTERYKDRWKSKPFNMTTDYNGAKFVLNFRLAG
                     LKNLGLQLPNFLRYIQLDFGYFTRNYIHRKETDAPPYRSLYFGISLNFMEVVKDFFDN
                     PESRTCKILQQPFKYYHIPAGYSGDYK"
     gene            1212..2525
                     /locus_tag="PLX22_06140"
     CDS             1212..2525
                     /locus_tag="PLX22_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT19513.1"
                     /translation="MKYNVALTLYEGKGSVAKNIESIHALKNLKPSDKVFIKPNIVFW
                     ATVPFPKWGVITTSSVLCEVVELLKDYGVSDITIGEGIVTLKPNDKETAAHAFEYLGY
                     NTLAKRYGTKIINVFDSSFEKVKLDDDIEINVSSDALHSDYLINIPVLKTHAQTVVSL
                     GLKNLKGLLNIPSRKKFHNPDETKNLHYMISRLYKLLPKGTTIIDGMYTLERGPGFDG
                     KPHRSNIIISSEDTLSADMVGAKVLGHQPDSIPYLAYAAAEEHRDADLSGINIIGTPV
                     TDVAHYHEYTFPYTDDGTLPQKLHAMGIQGLSYPKYDLTMCTYCAALNGAVLTAITLA
                     WQGKPWDEVEVLTGKKMQPGGNKKHTILLGQCMVNLHKHNTAINAIPVKGCPPNPMET
                     VTALHKAGIEVNPLIFEHLDSAPAYFMERYKNKPEFEEDFFTIKG"
     gene            3001..4221
                     /locus_tag="PLX22_06145"
     CDS             3001..4221
                     /locus_tag="PLX22_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOT19514.1"
                     /translation="MKTSFKVLSIAAMVLFAVANAFATNGMRMIGFGPVQRSMGGIGV
                     ATTLDAASIITNPAGIADLKGRIDFGASYFSPTVKHKASGPMIGIMAKDGKTVESDAG
                     ASPVPAFGLVIPLTEKLHFGIGAYGVSGMGVDYEKNVYQSILKTMYSQMRFAPGLSYK
                     INDMISVGAVANIMYATMEFSAATNASVIGMSDQEEHMNSAAFGYGATIAINIKPVDM
                     LTIGVAYETKSNFKDFEFNTAQGKDKLEFDQPQSVTAGVSVTPLKDLTVAFEVQWIEW
                     SETNGKNKPEFSKNSSNATPWNMNWDNQMVYKIGAQYAINPMFTVRAGFNYGKMPLDE
                     DRAFENVAFPAIAEYHYTAGIGITFDNNLTLNIGGMYSPEAKIDGANMSQAFAHYETK
                     MSQYSLDMGIAYVF"
     gene            4530..5405
                     /gene="rfbA"
                     /locus_tag="PLX22_06150"
     CDS             4530..5405
                     /gene="rfbA"
                     /locus_tag="PLX22_06150"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT19515.1"
                     /translation="MKGIILAGGSGTRLYPITKATCKQLLPVYDKPLIYYPLSVLMLS
                     GINDILIISTPQDSERFYNLLGDGSWLGIKLSYAVQEKPNGIAEAFLIGKNFIGNDTV
                     ALVLGDNILFGHGLIAHLLDSKTIVHTDNHAVIFAYYVKDPQQYGVVEFDEKLNAISI
                     EEKPAKPRSNYAVIGLYFYPNDVVKKAKSITPGNRGELEITSINQLYLDQNNLKVKVM
                     GRGFAWLDTGTHDSLLEASNFVKIIEERTGLKIGCVEEIAFKNGWITKEQVLLIAKSL
                     SKTDYSQYLITIAGK"
     gene            5406..6446
                     /gene="rfbB"
                     /locus_tag="PLX22_06155"
     CDS             5406..6446
                     /gene="rfbB"
                     /locus_tag="PLX22_06155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOT19516.1"
                     /translation="MDSILITGGCGFIGSNFIEYCIDKYDYHIINIDALTYAANPDNV
                     KEVANHKRYTFIHGNICDRELLQSIFHKYTIKGVIHFAAESHVDNSIKDAENFITTNI
                     VGTHALLQCAYRSWMEKPFVYKHDYRNCRFHNISTDEVYGSLGSSGYFTELSPYAPNS
                     PYSASKASGDMLVRAYHHTYGLNTVITNCSNNYGPKQHDEKLIPTIIRNALHGNPIPI
                     YGDGKNVRDWLYVLDHCKGIDVVFHKGKAGQTYLIGGHNEHDNNAIAKKICSLLDSMY
                     PVHNNSIINEGKRTISSYADLIHYVEDRPGHDKRYAIDPTKIETELGWKARENFEDSL
                     FTTVQWYITKYV"
     gene            6472..6990
                     /locus_tag="PLX22_06160"
     CDS             6472..6990
                     /locus_tag="PLX22_06160"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT19517.1"
                     /translation="MQTKKIIDHIHDAVYTRKSNLPFTVIATAMFGSYATDRQNIHSD
                     IDLLVVAEGIHPQLHRRASQIVLLKNMLSIGMPSDIILLTNDECISNFTNHNPLFLDI
                     ACDGIIIVDQDSFLSSLIDETKMYITNNKLQKLVDGWRFPVQYRRTTFLSSVSNKDFS
                     RAMLVNKNQKLL"
     gene            7083..8015
                     /locus_tag="PLX22_06165"
     CDS             7083..8015
                     /locus_tag="PLX22_06165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT19518.1"
                     /translation="MKNVYISVVTPVYKCAECLDELYTRLIASLHSITNNFEIIMVDD
                     GSPDNAWQIITSLATKDKRVNGIKLSRNFGQHYAITAGLDFAKGRWVVVMDADLQDQP
                     EEIVKLYNKAQEGYDIVVGKRVERKDTFIKKITSKLFFTIYSYLTDTNIDGSISNFGI
                     YSHKVINSIKSFKEHTRSFGLFAIWVGFKRIEIAIEHSNRKKGKSSYSIKKLFTLALD
                     SIISHSDKPLKLFLTSGFMLSFLSFLYAIFLVIQFYIFSIPVTGWTSLMVSIFFLGGI
                     IIACMGIIGLYLNKIYDEAKNRPLYIIAATTFKE"
     gene            8020..9159
                     /locus_tag="PLX22_06170"
     CDS             8020..9159
                     /locus_tag="PLX22_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT19519.1"
                     /translation="MDTTFTIGFIGGGLNSAVGITHKIASQMDGHFKLVAGCFSRHND
                     INIQTAEYFNMDKKRLYFNWKDLLENEKDKIDAIVILTPTPSHYEILEETIKNQYAII
                     CEKALVSSLEEGKKIKQLIHKYKNFLTVTFNYTGYPMIRELKHMINKGILGDINQIHI
                     EMPQETFIKVDKNGSPIYPQPWRQKDYDIPTVSLDLGVHLHSLIYFLTQKKPLEVVAV
                     ENSFGHFKQVVDNVISIIKYSDNMICNMWYSKSACGHRNGLRLRIFGTYGSAEWYQME
                     PEKLIVCDNKGNIQIVDRVSNLVEVTCLPRYNRFKAGHPAGFIEAFANLYEDIADTLQ
                     HYQQHKDTPVNYTFDIDIALEGLNLLNTINLSAKEKAFIKLEEVI"
     gene            9156..10223
                     /locus_tag="PLX22_06175"
     CDS             9156..10223
                     /locus_tag="PLX22_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOT19520.1"
                     /translation="MIPVHKQNINYEVIDFCEKFKHSKQPKLIFGINEFANSIAEAMD
                     IDGFIDDYTTTTVHLGKPIYKTETIPKNALVVNVVILGRPLTAKNKLNEQGVEHLDYF
                     AFYKYSRLKLPNVTFWDGFIQDFDNNRSKYEWVYSILSDEQSKQEFEKIINFRLSYDL
                     SNMHGFKNIQDQQYFDAILNIATCDVFVDIGGFDGFTSREFIKRNPHYKHIYFFEPDI
                     TNMEIAKELLKDFSGIQYYEMGLSNAKGNTNFCINGSSSKIDSSGTKIIYVDRLDNII
                     FEPIQYIKMDVEGYESSVLEGCEKIIKNYQPSLAVCAYHKPDDLWKIPEQVLSINKSY
                     KVYLRHYTEGITETVLYFLPD"
     gene            10268..11014
                     /locus_tag="PLX22_06180"
     CDS             10268..11014
                     /locus_tag="PLX22_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19521.1"
                     /translation="MIINKLLSHLNNLSLSQLPDFINKMIDPDTFKRKEFIYKCSQES
                     PEGSYILDAGAGQCHYKGFFNKQKYIATDLSVGDINWDYSKLDVLSNLETLPFKNNVF
                     HSIICINVLEHVKEPFNVISEFYRVLKPGGSLYLTVPQGWWLHQEPYDYFRYTNYGIS
                     YLLEKAHFFINDIKPTSGYFSYLANRITFLPKALFWTIKSKIIRILLLPFEIISYLLF
                     VLLIPIILNCIDGYDKQNKFTLHYMVKASK"
     gene            11020..>12162
                     /locus_tag="PLX22_06185"
     CDS             11020..>12162
                     /locus_tag="PLX22_06185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HOT19522.1"
                     /translation="MYTEWTKKSWQNFNYYQKANWYDTGLLADVLQELSKLPALVFAG
                     ETRSLKKDLIDISRGDAFLLQCGDCSESFVDCQGPRIHNLIRIILQMSMIITYITNKR
                     VVKIGRIAGQYAKPRSNEFEIVDNVQLPIYRGDMINSFLPAQEYRKHDPKRILEAYYK
                     SAATLNLIRAFTKGGYASLQSITDWQKHYFKNFPIMDKYSEIVINITKAINFMKALGL
                     DVNNPQFNEVNFYTSHEALILEYEEALTRIDTTTGDWYDTSAHMLWLGNRSRHCDSSQ
                     VEFLSGINNPIGIKVGPDYNDEDLICIIDKLNPANEQGKICIITRFGIPTINKQLPVL
                     IKHIKRKKNNVVWICDPVHGNTYVNKYNRKVRRFEDIVKEIESFWEI"
BASE COUNT         4116 a         2099 c         2276 g         3671 t
ORIGIN      
        1 ttttataatc gccgctatat cctgccggta tatggtaata tttaaacggt tgctgtaaaa
       61 ttttacaggt tctgctttcg gggttgtcaa aaaagtcttt taccacttcc atgaagttta
      121 atgatatgcc aaagtacagg cttcggtatg gtggagcatc cgtttccttc ctgtgaatat
      181 agttgcgggt aaaataacca aaatcaagct gtatatagcg caaaaagttg ggcaactgta
      241 aacccagatt ttttaaaccc gccaatctaa agtttaggac aaattttgca ccattgtaat
      301 cggttgtcat attaaaaggt tttgacttcc acctgtcctt gtagcgctcg gtgggccagt
      361 actgccagct aaatccaata agcgagtcaa gtgttggtga atattctaat agtatcccca
      421 gcccaatgcc tgtcatatca gagacaacat cttcatacga aaagccgtat tcgccggtga
      481 atgcatcgcc aacttctatt aagatcccta caaacgatgc tgttgtaatt gagtaaaacc
      541 acctggtact tttaccattt tcagaccaat cataatagtt atacaaagcc ctgaacatca
      601 catagtgcga aaaggcatgc cccaatttat cagcaccccc cgaataggtg tttttgccaa
      661 aaaaaccttc atcaccaaaa tgaggatttt tttgactgcc ccaatcccat gttaccgtac
      721 catagatagt gatagcaact ggaacagcaa taaacatggc aagggtagga gttgttggcc
      781 agttaccttc agtaaatggc acccagctgg catctgcatt agtcgttgat gtattttcct
      841 gtgcaaatac tgagcatgga gaaaaaaaaa gaagtgtaat agttactaat tgtatgagat
      901 gtttttccat gtaaatgttc ctcaaaatga atacaatatg acaaggaata acaaaaaaat
      961 attatgatat acaataccaa tcatactgtt aaaagtcaac ataattataa ctattttgat
     1021 atttttacaa atagcatatt ccatgcaata tgaaagcaaa actatttatt atacgataca
     1081 gttcaaactt atatttgcac atttgacctt ctccattatt atttttttaa aaaataaata
     1141 gcaattttac agggttgggc gatagttgtt tttaatttat tatcatttga aaatagtatg
     1201 tgaggtttat catgaaatac aatgttgctc tgaccctgta tgaaggcaaa ggttctgttg
     1261 caaaaaatat tgaaagcatc catgcactga aaaatcttaa gccgtcggac aaagttttta
     1321 taaaacccaa catagtattc tgggcaacgg ttccatttcc aaaatggggg gttattacca
     1381 catcatcagt gctttgcgag gttgttgaac ttctcaagga ttacggtgtt agcgatataa
     1441 ccataggtga aggtatcgtt accctgaaac caaacgacaa agaaacagca gcacatgctt
     1501 ttgaatattt aggctacaac acgctggcta aacgctatgg aacaaaaata ataaatgtgt
     1561 tcgatagttc ctttgaaaag gtaaagcttg atgacgacat tgaaattaat gttagcagtg
     1621 atgcactgca cagtgactat ctcataaata ttcctgtttt gaaaactcat gcacaaaccg
     1681 ttgtgagttt gggcttaaaa aatttgaaag gacttcttaa catcccatca cgaaaaaaat
     1741 ttcacaaccc ggatgaaaca aaaaatcttc actacatgat atctcgctta tataagctgc
     1801 tgccaaaagg aacaaccatc atcgatggca tgtatacatt agagcgcggg ccaggctttg
     1861 atggcaaacc acaccgaagt aatattatca tctcatcaga ggatacgctt tcagccgata
     1921 tggttggtgc aaaggtttta ggtcaccagc cggatagcat accttattta gcctatgcag
     1981 cagcagagga gcatagggat gctgaccttt ccggtatcaa catcattggc acgcctgtaa
     2041 ccgatgttgc acactaccat gagtatacat tcccctatac agacgatgga acactccctc
     2101 aaaagctgca tgcaatggga atacaggggc tgtcataccc taaatatgat ttaaccatgt
     2161 gcacctattg tgctgctctc aacggagctg tgctgacggc aattaccctt gcatggcagg
     2221 gcaaaccatg ggatgaagta gaggtgttaa ccggtaaaaa aatgcagcca ggcggcaaca
     2281 aaaagcacac catattgctt ggacaatgta tggtaaatct gcataagcat aacactgcaa
     2341 ttaatgcaat accggttaaa ggctgtcctc ccaaccctat ggaaaccgtt acggcattgc
     2401 acaaagcagg cattgaggtt aatccgctta tctttgagca tcttgatagt gcacccgcct
     2461 attttatgga acggtataaa aacaaaccag agtttgaaga ggattttttt actataaaag
     2521 gataatcagt cttaaaaaaa tttactggca cgcgccgcat cctttgattc agagtttacc
     2581 aattcaatga tgattgatca tgatattgca atgatgatga aagaaacagt atcgtcataa
     2641 aaagatggag agcccctata aaaacaacaa ccgttattga tgacatcgtg taatcatgaa
     2701 agtagccctt ccatagtaag atagagtaag atagtgagta aaaacaataa atgaaccggg
     2761 tgatgcactc tgaacaattg taatagtgag agcgtacatt tgtgacttga atccaagact
     2821 gtcaataatc attcattttc ccaattatta agtattatag tataatttaa ttatacaaaa
     2881 tttttattta ataatattat aaaattcttg acatttaaac aaccccttgc tacattgatt
     2941 gtatctaaaa aatctattat aataacaata aagtttaaat aacttcgtga ggagggtttt
     3001 atgaaaacca gttttaaggt tttatctatt gcagccatgg ttttgtttgc agttgcaaat
     3061 gcatttgcca ccaacggcat gcgcatgatt ggttttggtc cggtgcagcg ttctatgggt
     3121 ggtattggag ttgccaccac gcttgatgcg gcttccatca ttacaaatcc agcagggata
     3181 gctgatttaa aagggcgtat tgattttggc gcttcatatt tttcaccaac ggtaaagcat
     3241 aaggcttccg gtccaatgat agggataatg gcaaaagacg ggaaaaccgt tgaatcagat
     3301 gcaggcgctt caccagtacc ggcatttggg cttgttatac cgctaaccga aaagttacat
     3361 tttggtattg gcgcctacgg tgtttccggt atgggcgttg actatgaaaa aaatgtttac
     3421 cagagtatct taaaaaccat gtatagtcag atgcgctttg ctccagggtt atcctacaag
     3481 ataaatgata tgatatcggt tggcgctgtt gcaaacatta tgtatgcaac aatggaattt
     3541 tcagcggcaa ccaatgccag tgtaatagga atgtctgacc aggaagagca catgaattct
     3601 gccgcatttg ggtatggagc aaccattgct atcaacatta aaccggttga tatgcttact
     3661 attggtgttg cctatgaaac aaagagcaat tttaaggatt ttgaatttaa taccgcccag
     3721 ggcaaagata aattggaatt tgaccagcct cagagtgtaa cagcaggtgt ttcagtaacc
     3781 ccattgaaag accttactgt agcgtttgag gttcagtgga tagaatggtc cgaaacaaac
     3841 ggcaaaaata agcctgaatt cagcaaaaac agcagcaatg caacgccatg gaacatgaac
     3901 tgggacaacc agatggtgta taaaataggt gcccagtatg caatcaatcc aatgtttacc
     3961 gtgcgagcag gctttaacta tggcaagatg ccgttagatg aagacagagc atttgaaaac
     4021 gtggcattcc ctgctatagc cgaatatcac tataccgctg gtattggcat aacatttgat
     4081 aacaacctta cgcttaatat aggcggcatg tattcacctg aagctaaaat tgacggtgct
     4141 aatatgtctc aagcttttgc ccattatgaa actaaaatga gccagtattc acttgatatg
     4201 ggcatagcgt acgtattcta aaaagattta tactgttacg taatcccatg aaatgagagg
     4261 gctatccctc tcattttttt attacctaca agtcctatga aggcgaggaa acaggggtga
     4321 ggtttcccct tgccttacca gtgagatggg ccgtggttta tctttaggtt tttaaaatcc
     4381 ctttcaatac ttattataaa acgcttaata gttatataat tttccctaac cagcataaaa
     4441 taataattga cacaaacaat ttttttcgta aacatttatc agtaataaca ttcgttaatt
     4501 ttaataaaat aaagagtaat ttcaccaata tgaaggggat tatattagca ggcggttccg
     4561 gaacacgcct gtatccaatt acaaaagcaa catgcaagca gctattgcct gtttatgata
     4621 aaccccttat ctattatcct ctttcagttt tgatgctttc cggtattaat gatatactca
     4681 taatttcaac acctcaggac agtgaacgtt tttataatct tctgggtgat ggcagctggc
     4741 tgggaataaa actatcgtac gctgtgcagg aaaaacccaa tggtattgca gaggcttttt
     4801 taattggtaa aaattttata ggcaacgata ctgttgccct ggtattgggt gacaacatac
     4861 tctttggaca tgggctcatt gcacatttac tggacagcaa aacaatagtc catactgaca
     4921 accatgctgt catctttgcg tattatgtta aagaccctca acaatacggt gttgttgaat
     4981 ttgatgaaaa gctcaatgcc atctcaatag aggaaaaacc agcaaaaccc aggagcaact
     5041 atgctgttat tggactgtac ttttacccca atgatgtagt aaagaaagcc aaaagcataa
     5101 caccaggcaa ccgtggcgag cttgaaatta ccagtattaa ccaattatat ttagatcaaa
     5161 ataatttgaa ggttaaggta atgggaagag gttttgcatg gcttgatacc ggtacccatg
     5221 atagccttct ggaggcaagt aactttgtaa aaattattga ggagcgtacc gggctaaaaa
     5281 ttggctgtgt ggaggagata gcatttaaaa acggctggat tacaaaagag caggttcttt
     5341 tgattgcaaa gtcactatcc aaaacagatt atagccaata tctaattact attgcgggaa
     5401 aataaatgga ttctatttta attaccggtg gctgtggttt tattgggagc aattttattg
     5461 aatattgtat tgataagtat gactatcaca ttatcaatat tgatgcgctg acctacgctg
     5521 caaaccccga caatgtaaaa gaggtagcta accataaacg ctataccttt atccatggta
     5581 acatctgtga cagagagttg ctgcagtcca tctttcataa atatactatc aaaggtgtca
     5641 tccactttgc tgctgaaagt catgtggaca actccatcaa agatgccgag aactttatta
     5701 ccaccaatat cgttgggacg cacgctcttt tacagtgtgc ctatcgctcg tggatggaaa
     5761 aaccttttgt ctataaacat gattaccgga actgtcgctt ccataatatt tcaaccgatg
     5821 aagtgtatgg ctcgcttggc agtagcggct attttacaga actatcgccc tatgccccca
     5881 acagtcctta tagcgccagc aaggcatccg gtgatatgct ggtgcgagcc tatcaccaca
     5941 cctacggatt aaataccgtt attaccaatt gctcaaacaa ctatggtcca aagcagcacg
     6001 atgaaaagct tattcccacc atcatacgaa atgcgttgca tggaaatccc atacccatat
     6061 atggcgatgg caaaaatgtc agggattggt tatatgtgct tgatcattgt aaagggattg
     6121 atgttgtttt tcataaaggc aaagcgggac aaacatacct cattggcggg cataatgagc
     6181 atgacaacaa tgccattgca aaaaaaatat gttcattgct tgacagcatg tatccagtgc
     6241 ataacaattc aataatcaat gagggtaagc gtaccatatc ctcgtatgcc gatttaatac
     6301 actatgttga agaccgtccg gggcatgata agcgctatgc tattgacccc acaaagattg
     6361 aaacagagct tggctggaag gcacgtgaaa attttgaaga tagtttgttt actaccgttc
     6421 aatggtatat tacaaaatat gtttaagcga tagtaactat atagtattac catgcaaact
     6481 aaaaaaatta ttgatcacat tcatgatgca gtgtacaccc gaaaaagcaa tcttcctttt
     6541 acggtaattg ctactgcaat gtttggttct tatgctaccg acaggcaaaa tattcattcg
     6601 gacattgacc ttctagttgt tgccgaaggc attcatccgc aactgcatag acgggcaagt
     6661 cagattgttt tattaaaaaa catgctatca ataggcatgc catcagatat tatactgctt
     6721 acaaacgatg aatgtatttc taactttacc aatcataatc cgcttttttt agacattgcc
     6781 tgtgatggaa tcattattgt tgatcaggat agctttttgt cgtcgttaat agatgaaact
     6841 aaaatgtata ttacaaacaa taaattacaa aagctggttg acggatggag attccctgta
     6901 cagtatcgtc gcacaacctt tttgtcatca gtcagcaata aagatttttc cagagcaatg
     6961 cttgtcaata aaaatcaaaa attgttatag cagttgctta taactttgtt acatggtggt
     7021 ttgttgatta acgcagtatg tattttttta tgatgtaatt ttaatgatat aaggttttgt
     7081 ttatgaagaa tgtttacatc tcggtggtta ccccggttta caaatgtgca gagtgtctgg
     7141 atgagctgta cactcgttta attgcatcat tacactctat tacaaataat tttgagatta
     7201 tcatggtgga cgatggcagt cccgacaacg catggcaaat cataacatcg ctggctacaa
     7261 aggataaacg ggtaaatggt ataaagcttt cacgcaattt tggacagcat tatgccatta
     7321 cagcagggct tgattttgca aaaggcagat gggtggtggt tatggatgct gatttacagg
     7381 accaacccga agagattgtt aaattgtata ataaggcaca ggaaggatac gatattgtag
     7441 tgggaaagag agtggaaagg aaagatacat ttataaaaaa gattacctca aaactatttt
     7501 tcacaatcta ctcgtatcta actgatacaa atatagatgg aagcatcagt aattttggca
     7561 tatactccca taaagttatt aatagtataa agagttttaa agagcatacc cgttcatttg
     7621 gtttgtttgc aatctgggta gggtttaaaa ggattgaaat tgctatagag cattccaaca
     7681 gaaagaaagg taaatcaagc tatagcatta aaaaattatt tacattagct cttgacagca
     7741 ttatatcaca ttctgataaa ccattgaaat tgtttttaac atcaggcttt atgttatcgt
     7801 ttctctcatt tttatatgct atttttcttg ttatacaatt ttatatcttt tcaatcccgg
     7861 taaccgggtg gacaagcttg atggtttcaa ttttttttct tggtggtatt attattgcct
     7921 gcatgggtat tattggttta taccttaata aaatatatga tgaagcaaaa aacaggcctt
     7981 tatatataat agcagctaca acatttaagg aataattaca tggatacaac ctttactatt
     8041 ggatttatag gcggtggact aaattcggca gttggtatta cccataagat agcatcacaa
     8101 atggatgggc actttaaact tgtggcgggc tgttttagca gacataatga tataaatata
     8161 caaacggctg aatactttaa tatggacaaa aaaagattat attttaactg gaaagatctt
     8221 ttagagaatg aaaaagataa aattgacgcc attgttattt taaccccaac cccatcgcat
     8281 tatgaaattt tagaagagac aataaaaaat cagtatgcaa taatatgtga aaaagcactt
     8341 gtttcttcgc tggaagaagg taaaaagatt aaacagctaa tacataaata taaaaacttt
     8401 ttaacagtaa cctttaatta cacaggttac cccatgatac gagaattaaa acatatgata
     8461 aacaagggaa tattagggga tattaaccaa atacacattg aaatgccgca ggaaaccttt
     8521 ataaaagttg ataaaaatgg tagcccaata tacccccaac catggcgaca aaaagattat
     8581 gatataccta ctgtatccct tgacctggga gtacatctgc atagtttaat ttatttctta
     8641 acacaaaaaa aaccattaga ggttgttgcc gttgaaaatt cttttggtca ttttaaacag
     8701 gtagttgata atgttattag cattattaaa tattcggata acatgatatg caacatgtgg
     8761 tacagtaaat ctgcctgtgg acacaggaat ggattgcgat taagaatttt tggcacttat
     8821 ggttcggctg aatggtatca aatggaacct gaaaagctta tagtatgtga taacaagggg
     8881 aacatacaga tagttgatcg tgtaagcaac cttgttgagg taacatgttt accccggtat
     8941 aaccggttta aggcaggaca tcctgcaggt tttattgaag cttttgcaaa tttatatgaa
     9001 gatattgctg atacattgca acattatcaa cagcacaaag atactcctgt aaattatact
     9061 tttgatatag atatcgcact tgaaggatta aaccttttaa atacgataaa cctttcagca
     9121 aaagaaaaag cattcataaa attggaagaa gttatatgat tccagtgcat aaacaaaata
     9181 tcaattatga agtaatagat ttttgtgaaa agtttaaaca ttcaaagcaa ccaaagttga
     9241 tttttggaat taatgagttt gccaattcga ttgcagaagc aatggatatt gacggtttta
     9301 ttgatgatta tacaacaact acagtacatt tagggaaacc aatttataaa actgaaacaa
     9361 taccaaaaaa cgctttagtt gtaaatgtag taatattagg aaggcctctg acagccaaaa
     9421 ataaattaaa tgaacagggt gttgaacatc ttgattattt tgccttttat aaatactcgc
     9481 gtttaaagct cccaaatgtt accttctggg atggttttat tcaagacttt gacaataata
     9541 gatcaaagta tgaatgggta tattctattt tatctgatga acaatccaaa caagaatttg
     9601 aaaagataat aaatttcaga ctgtcgtatg atttatctaa tatgcatggt tttaaaaaca
     9661 ttcaggatca acaatacttt gatgcaatac tgaatattgc aacatgtgat gtatttgttg
     9721 atataggtgg atttgatgga tttacaagcc gtgaatttat aaaaagaaat cctcattata
     9781 aacatatata tttttttgaa cctgacataa caaatatgga aatagcaaaa gaactgttga
     9841 aagatttttc aggaatacag tattatgaaa tgggattgtc aaacgctaaa ggtaatacaa
     9901 atttttgtat taatgggtca agttcaaaga tagattctag tggtacaaaa ataatttatg
     9961 tagataggct tgacaatatc atttttgaac ctatccagta tattaaaatg gatgttgagg
    10021 gttatgagtc aagtgtactg gaaggttgtg aaaaaattat taaaaattac cagccatcat
    10081 tggcggtgtg cgcatatcac aaacctgatg atttatggaa aattcctgag caagttttat
    10141 ccattaataa atcatataaa gtatatttac gccattatac cgaagggatt accgaaactg
    10201 ttttatattt tttaccagat taatttgtaa caacaatgtt attattaaaa ttcaaagttg
    10261 gcttgctatg attattaata aattactatc acacctaaac aatctatctt tatcacagct
    10321 tcctgatttt ataaataaga tgatcgatcc tgacaccttt aaacgaaaag aatttattta
    10381 taaatgctca caggagtcac cggaaggatc atatatcctt gacgcaggtg ccgggcagtg
    10441 ccactataaa ggcttcttta ataaacaaaa atatattgcc actgacctta gtgtaggcga
    10501 tataaactgg gactatagca aattagatgt attgagtaat cttgaaacat tgccatttaa
    10561 aaataatgtc ttccacagta ttatttgtat aaatgttctg gagcatgtaa aagaaccatt
    10621 caatgtaatt tctgaatttt atagagtttt aaaacctggc ggttcattgt accttactgt
    10681 accgcaggga tggtggttgc atcaagaacc atacgattat tttcgctata caaattacgg
    10741 gatatcatac ttgctggaaa aggcccattt tttcattaac gatatcaaac caacatcagg
    10801 ttatttcagc tatctggcaa acagaattac ctttttgcct aaagctttgt tctggacaat
    10861 aaaaagcaaa ataatacgaa ttttactgct accttttgaa atcatttcat atcttttatt
    10921 tgtactgctt atcccaataa ttcttaattg tatcgatggt tatgataaac aaaataaatt
    10981 tacactacac tacatggtaa aagcatctaa ataaaaagta tgtatactga atggacaaaa
    11041 aaatcatggc aaaattttaa ttattaccaa aaagccaatt ggtatgacac cggactttta
    11101 gcagatgttt tacaggaatt aagcaaactg ccggcattgg tatttgctgg tgaaaccaga
    11161 tctctgaaaa aagacttaat tgacatatca aggggagatg catttttgct acaatgcggt
    11221 gactgttctg aaagttttgt agattgtcag ggacccagaa tccataatct catacgtatt
    11281 atcttacaga tgtccatgat tataacttac atcacaaata aaagagtggt taaaattgga
    11341 agaattgcgg gtcaatatgc aaaaccacgt tcaaatgaat ttgaaatagt ggataatgtt
    11401 caattgccaa tttacagagg tgatatgatc aatagctttt tgcccgcaca ggaatatcgt
    11461 aaacatgacc caaaaaggat attggaagct tattataaat cagcagcaac attaaactta
    11521 ataagagcat ttacaaaggg aggatacgca tcccttcagt caatcacaga ttggcaaaag
    11581 cattacttca aaaattttcc tataatggat aaatattctg aaattgttat aaacataaca
    11641 aaagcaatta actttatgaa agcattgggg ctggatgtaa ataatcctca atttaatgaa
    11701 gtaaactttt atacatcaca cgaagcttta atacttgaat atgaagaagc acttacaagg
    11761 attgatacaa ccaccggtga ttggtatgat accagtgctc atatgttgtg gttaggcaac
    11821 agaagccgtc attgcgatag ttctcaggtt gaatttttga gtgggatcaa taatcctatt
    11881 ggtattaaag ttggaccgga ttataacgat gaagatttaa tttgtataat tgataaactt
    11941 aatcctgcta atgaacaagg aaaaatatgc attattactc gctttggtat acctacaata
    12001 aacaaacaac ttccagtttt aataaaacat attaaacgca aaaaaaataa tgtggtatgg
    12061 atatgcgatc cagttcatgg taatacgtat gttaataaat ataatcgcaa agtgcggcgt
    12121 tttgaagaca tagtaaaaga aattgaatca ttctgggaaa tt
//
LOCUS       DAOSOX010000063        12161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5211_length_12161_cov_8.421609, whole genome shotgun sequence.
ACCESSION   DAOSOX010000063 DAOSOX010000000
VERSION     DAOSOX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_12161_cov_8.421609"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLX22_06190"
     CDS             <1..542
                     /locus_tag="PLX22_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT19523.1"
                     /translation="INKNEHGLTLHCTSGRTIDAGTVIFSMGIKARTDLAKNAGLTVN
                     RGIIINNYLQTSDPYIFAAGDPSEHNGICYGIWTASREQGKLAGLNMAGVNNEYNGTL
                     LSVVLKITGIDLYSAGDFTRKDCTVLSQRDTSRYVKFLYKEKTPVASIVIGDMNIIKL
                     ARNVMEGKTSIDEFKALFT"
     gene            complement(712..1869)
                     /locus_tag="PLX22_06195"
     CDS             complement(712..1869)
                     /locus_tag="PLX22_06195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT19524.1"
                     /translation="MSYQTMISQRANEFTSFIVMDVMAKAEELERKGEHVIHLEVGEP
                     DFQTPEVIEQAAIEALKKQKTKYTHALGLLELREAISEYYYNEYNVSVSPDRILVTTG
                     TSPALLFVMLAMLNHGEDIIVSNPRYPCYQNFIKAAGGRVNEIVTYPEEGFQYRPEDI
                     NRAITTKTKAIMINSPCNPTGIVMDEHALKNIAQFQNQFIISDEIYHGLVYNNKAHSI
                     LEFTNKAFVINGFSKLYAMTGWRLGYCIFPPEFTMVMQRIHQNFMISANNFIQWAGIA
                     ALKYAKNDVKAMQTIYNERRQYMIQGLKRLGFIVHVEPTGAFYVFADARTFCSDSYSE
                     AFNIIETVKVGVTPGIDFGSAGEGFLRFSYATSIENIEEGLKRIEQYLKNR"
     gene            2148..2639
                     /locus_tag="PLX22_06200"
     CDS             2148..2639
                     /locus_tag="PLX22_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT19525.1"
                     /translation="MVQVLILYYSRSGNTHELAKAIAEGVKQVENAQAIVRSTSEVTK
                     DDFLNSHGIIAGSPVYFGTMAAELKHLFDEYVIIRRKMENKIGAAFATSSDPSGGKET
                     TLLSILQAMLIYGMIIVGDPMEATGHYGVSCTGSPDEKTKKNAAALGKRVASLAKRIW
                     EQQ"
     assembly_gap    2712..2811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2812..3489
                     /gene="ispD"
                     /locus_tag="PLX22_06205"
     CDS             <2812..3489
                     /gene="ispD"
                     /locus_tag="PLX22_06205"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT19526.1"
                     /translation="LAGGLGQRMDSAVPKQFLALNDKPIIIWAIETFYTSSLFDAIII
                     AIHPDYHKTLQSLLCDYNIQTTVIITNGGKTRQQSCYNALCSLQCNDNDIVLIHDAAR
                     PFITQTMIQNCINTTVQYGACGIYTPVKETIAMIKDDSVVEVLPRHAICAAQTPQCFY
                     YSLIMDAHKKALAHSINDATDDISLVLIAGHPVKAITGNYYHNIKITTIEDIAIAQAI
                     INNNLYH"
     gene            complement(3759..4463)
                     /locus_tag="PLX22_06210"
     CDS             complement(3759..4463)
                     /locus_tag="PLX22_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT19527.1"
                     /translation="MNVVIDSVTGLDIVHPFTVLMRILCALVAGFIIGFERERNYQPA
                     GLRTHMVLSLGACTVMILSYFIPYAYSPQNADPARLSAQVISGIGFLGAGAIFKYGFN
                     IKGLTTAATIWTTSGIGMVFGAGFYFLGVIATLFLVITLQLFEKVEMWLVEQKKTRIL
                     QVTYYSDAISGKEILTAIKKHTGDIDVRQVSVTEDVENKTSDFVVNCRIDEDFSLRLL
                     FDEIKQLGHIKKIKID"
     gene            complement(4447..5838)
                     /gene="argH"
                     /locus_tag="PLX22_06215"
     CDS             complement(4447..5838)
                     /gene="argH"
                     /locus_tag="PLX22_06215"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT19528.1"
                     /translation="MGKKKLWGGRFSNAPSKAAEEIATSLHYDMRLYKHDIRGSLAHA
                     KMLCKQKIISQKDCDDIQKGLSIIEQEIENGKFPFNETLEDIHMNIEARLTELIGDAG
                     KRLHTARSRNDQVITDVRLYLKDEMYEIKKLLIQLIVTIVQKAEETKHIVMPGYTHLQ
                     IAQPVRMAHHLLAYAWAMLRDVQRLEFAYVQTDELPLGSGALAGVNYTTDRQFLADEL
                     HFGAIAHNSMDAVSNRDFIADFLYFSSIMGMHCSRLAEDIILWSTAEFNFITLSDSVT
                     TGSSIMPQKRNPDVAELVRGKTGRLYGNLITLLTLLKGLPMTYNRDLQEDKEPLFDAI
                     DTVKLIASGMNEVITTMHVNGEVARKAIYKNFSTATDIADYLVMKGTPFRQAHEIVGS
                     IVKYCEQHNVDFFTLPLEALKKFSEQFDNDIYDYINPEKSTERKQSYGSTSLKSVEHQ
                     IMVIREKLHECGY"
     assembly_gap    5855..5954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6320..7303)
                     /locus_tag="PLX22_06220"
     CDS             complement(6320..7303)
                     /locus_tag="PLX22_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19529.1"
                     /translation="MQLLINDHPVDFAVEKETTVKDIMGSILNWASERSLVFSGAIVD
                     GTVYQLDNAPDISIEKVNNINCYVQSVSDIVIDSIKEGIAYCNRACQHLENKSNTSLS
                     ADELLQLQNGFEWIVSVLQSVLHMLGLERDDLRFKDMTVQEFIGSVYSFGHNISSSTD
                     INLLTTANNILHDTMTLFRVLLCSDALKSLVVQSVDSPDVLFQTMVDLKGMVGKQVKN
                     LEEIAGLYQKGNDSEALEIIHTLVDFLYLYMRTCYQVSPVFNINIAEFIYNGQSLEEL
                     NIKIQDMLTEIVSVMENNDVVSLTDILEYELKPLINEIDHYIDILLQNIKS"
     gene            complement(7317..9341)
                     /gene="fliD"
                     /locus_tag="PLX22_06225"
     CDS             complement(7317..9341)
                     /gene="fliD"
                     /locus_tag="PLX22_06225"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOT19530.1"
                     /translation="MPVTLGGVASGMDTDQIIQKLVEVEARPILQWQEEKDRYSLRKE
                     ALRQLQTKLTTLNTATKDLYGFRSPFKEKGVRISDPEVLQAQASRFAENGTHTLEVVN
                     LASTQKISTDQIKEDEILPPGTFSIEVDGNSYTIKFRGGNLKSLSDKINEIASSVVSA
                     TTIRTVDDKGILSIESKISGKKGELKLRGDEEYLKKIGLVKGEKGAEKEFQKIIFDTK
                     YFTAYTGEKPIEDEDGVLSVDKEGKSVIIDAKLWREYILPVEYQTKKQSVLEVNVTYS
                     KEKSEEEVIPYRLEVGPDETTVIKGIELKGYNVSRERPLEKKQKKEIADIIGVGVISV
                     EDGKRSERIYPIDPNKQGKQEIPIGQDFGEKKITKILFYCNDGKAIFQDAALVTEIKG
                     EGILEPKNVITQADDAKLKLNGIEIVRQKNSGIDDVLKGVTINLMKPSTKPLTITIKQ
                     NTEAAIEKIKKFVEAYNDYLTFNNELTKAVKSDKLGDYQKDKYKNGIFMGDMSLMRLQ
                     NALKTAVTEAYPSNVEKPVKMLYQMGVSTGEINAKWETIKEGKLIIDEAKLVEILSEN
                     PEGVQEFFGSDNDGDNRIDNGFAYSIESILDPYVSAGKNIIVSKMDLEDASIKATNNR
                     IERKQEHLKTYEQKLRQKFASMEKAISGSKAQSNWLDMQMKNLPKDNDNK"
     gene            complement(9452..9781)
                     /locus_tag="PLX22_06230"
     CDS             complement(9452..9781)
                     /locus_tag="PLX22_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOT19531.1"
                     /translation="MDISKVTGNMSVNTFKVKQQEYKNEEKRLYEESKFTRENLDDAV
                     EMLNATAKVINNRIAFSVDEKTDRVVMRVVDVDNNEIIREIPPKEMIRLAAQIKDMIG
                     MFVDESR"
     gene            complement(9895..10737)
                     /locus_tag="PLX22_06235"
     CDS             complement(9895..10737)
                     /locus_tag="PLX22_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT19532.1"
                     /translation="MRINHNLSAIFANRQLKIVSSRMDKSIERLASGQQINRAGDDAS
                     GLAVSEKMRTQINGLFQAGRNAENGLSFIQVAEGSLQQINDILQRIRMLSVQAAHGIY
                     SNSDRAQIQVEVSQLIDEIDRISTSAEFNRMKMLTGIYARNSRTGSIFFHVGPNQGQR
                     IRAYITTMSAKALNLYDGNRKRSISTVGQANAMIGYIDAALDKLNRQRADLGGYYNRM
                     ENTIYALAMSYENMLAADARIRDADMAAEMVEFTKDQILVQSGVAMLAQANFKPQLVL
                     HLLG"
     gene            complement(11047..11895)
                     /locus_tag="PLX22_06240"
     CDS             complement(11047..11895)
                     /locus_tag="PLX22_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT19533.1"
                     /translation="MIINHNMSAVNANRTLKFTQWSVNRSMEKLSSGERINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLAQTAQIIQRIRVLAVQAANGIY
                     TNADRQLIQVEVSALVDEVDRIASQAEFNRFKILTGEFSKVNPKSSMWFHMGPNANQR
                     VRVFIGTMTAQAFRMKDATGKVAITLTSPGGANASIGIMDAALQKLSKQRADLGAYYN
                     RLEHTAKGLMTAYENVQASESRIRDTDMAEEMVDFTKNTVLVQSGTAMLAQANLQPQS
                     VLRLLV"
BASE COUNT         3524 a         2582 c         1990 g         3865 t
ORIGIN      
        1 caatcaataa aaatgaacac gggctaacac tgcactgtac atcgggaaga actattgatg
       61 caggcacggt catattctca atgggtatta aggcaagaac tgatttagct aagaatgcag
      121 ggcttactgt aaaccgtgga attatcatca ataactatct gcaaacatcc gatccatata
      181 tttttgctgc tggtgatccc agcgagcata acgggatatg ctatgggata tggactgcat
      241 cacgcgaaca gggtaaatta gcagggctca atatggctgg cgttaacaat gaatataacg
      301 gcacgctgct atcggtagta ttaaaaatta ctggtattga tctctattcg gctggtgatt
      361 ttacccgcaa agactgcaca gtgctttcac aaagagatac atcacgatat gtaaaatttt
      421 tgtataaaga aaaaactccg gttgccagca ttgttattgg cgatatgaac atcattaaac
      481 ttgcccgcaa tgttatggaa ggcaaaacca gcatagatga atttaaagca ctgtttacat
      541 agaggcagta tatatattgt atagtatatc gtgtatcgcc tgcttaaaaa ttttttttaa
      601 tcacatctca ccgcagtgct gtcatttcat aattaaaaat tttctatgcg atagttactg
      661 taaatatatt gagcaattaa aaaaataaac tgtcagcatc aatgatcagc attacctgtt
      721 ttttaaatat tgttctatcc tttttaatcc ttcttcaata ttttcgatag atgttgcata
      781 agaaaaacgt aaaaagccct cgcctgcact accaaaatca atgccgggag taacaccaac
      841 tttcaccgtt tcaattatgt taaatgcttc actatatgaa tcactgcaaa atgtacgagc
      901 atcagcaaaa acataaaacg cgcccgttgg ttcaacatgg actataaagc ccagcctttt
      961 taaaccctgt atcatatact gccttcgctc attataaata gtttgcatgg ctttaacatc
     1021 atttttagca tacttcaatg cagctattcc tgcccattga ataaagttgt ttgccgatat
     1081 cataaagttt tgatgtatgc gctgcataac catggtaaat tctggaggaa atatacagta
     1141 acctaaacgc catcctgtca tggcatacag cttggaaaat ccattaataa caaacgcttt
     1201 attggtaaac tccaatattg aatgtgcttt attgttatag acaagtccgt gataaatctc
     1261 atcggatatg ataaactgat tttgaaactg tgcaatattt ttcaatgcat gctcatccat
     1321 tacaatccct gttggattgc atggcgaatt tatcatgata gcttttgttt ttgttgttat
     1381 agccctgtta atatcttcag gcctgtattg aaatccttct tcagggtaag tgactatttc
     1441 atttaccctt cctccggctg ctttaataaa attttgataa cagggataac gcggatttga
     1501 tactataata tcttcaccat gattaagcat ggctaacatt acaaaaagta atgcaggtga
     1561 cgttccggta gtaaccagta tccggtcagg cgaaacagaa acattatact cattataata
     1621 gtattcactg attgcctcac gcaattccag caaacccagc gcatgggtat attttgtttt
     1681 ttgttttttt aatgcttcaa ttgcagcctg ttcaatcacc tccggtgttt gaaaatcagg
     1741 ctcaccaacc tcaagatgga taacatgttc gcctttgcgc tccaattctt ctgcctttgc
     1801 catcacatcc attactataa aggatgtaaa ttcatttgcc ctttgtgata tcattgtttg
     1861 atagctcata gttatttttc tcattcgata acaattgtat gtgcacatac acactgtaaa
     1921 acatacttgc attgcaggtg tgatagtgtt tgtaatgaca ctgaaaagct atataaacat
     1981 ctataatagt agctgacaag ctaaatttta cagtaactat cgcaaaaaaa acacaggtaa
     2041 ctgttgacaa aattaaaaat actattatta tttatatagt ataaatcata tacagtaaat
     2101 taatttccaa taattgtatt tcattacagt attatacgag gtataacatg gtgcaggttt
     2161 tgatattgta ttactcacgt tcaggaaaca ctcatgaact tgcaaaagcc attgccgaag
     2221 gagtaaaaca ggttgagaat gcacaggcta tagtgcgttc aaccagcgag gttaccaaag
     2281 atgatttttt aaattcccat ggtattatag ccggttcacc tgtttatttt ggaacaatgg
     2341 cagccgagtt aaaacatctt tttgatgaat atgtcattat ccgaagaaaa atggaaaata
     2401 agattggagc ggcatttgct acatcaagtg atccatctgg agggaaagag acaacactgc
     2461 tttcaatact gcaggcaatg ctcatctatg ggatgataat agttggcgat cccatggaag
     2521 caaccggtca ttatggagta tcatgcactg gttcgcctga cgagaagaca aaaaagaatg
     2581 ctgcagcact gggaaagcgg gttgcatcac tggcaaaacg catctgggaa caacaatgaa
     2641 acactatgca attatattag ccgggggact gggacagcgg atggatagtg ctgtcccaaa
     2701 acaatttttg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttagccggg
     2821 ggactgggac agcggatgga tagtgctgtc ccaaaacaat ttttggcact gaatgacaaa
     2881 ccaattatta tatgggcaat agaaaccttt tatacatctt cgctgtttga tgctattatt
     2941 atagccattc atcctgacta tcacaaaaca ctccagtcac ttttgtgcga ttacaatatt
     3001 caaaccactg tcataataac caatggaggt aaaacccggc agcaatcctg ctataatgcg
     3061 ctctgttcac ttcaatgcaa tgataacgat attgtgctca ttcatgatgc tgcacgccct
     3121 tttattacac aaaccatgat tcagaactgc attaatacta cagtgcaata cggcgcctgc
     3181 ggtatctaca caccggtcaa ggaaactatt gccatgataa aagatgacag cgtggtagaa
     3241 gttttacctc gacatgcaat ctgtgcagca caaacgccac aatgctttta ttattctcta
     3301 atcatggatg ctcataaaaa agcacttgca cattctatta atgacgctac tgatgatatt
     3361 tcattggttt tgattgccgg acatcctgtc aaagctatca ccggtaatta ttatcacaac
     3421 attaaaatca caacaattga agatattgct atagcacagg caataattaa caataatctt
     3481 tatcactgat acagcatgca gtagtatata aaatcttatt tctttttttc aaaatagttt
     3541 acattgggta aatccttgaa atgaacatca tgagtccacc cggaagaatc cacgatattc
     3601 ataatctgta gtacaatttg aaatttacag aaaacaatta caaaatccct atttacctca
     3661 ttcgattaaa tcttttctaa ggtaactatc tataaggaca ttaccagcaa gaattatagt
     3721 tatttgcaaa ctatggatta attgtataat tgtatatttt aatctatttt tatctttttg
     3781 atatgtccta actgttttat ctcatcgaac agtaaccgca ggctaaaatc ttcatctatt
     3841 cggcagttta ccacaaaatc cgacgtttta ttttcaacat cttctgttac cgaaacctgg
     3901 cgcacatcta tatcaccggt atgttttttt atagctgtaa ggatctcttt gccacttata
     3961 gcatctgaat aatacgttac ctgcaaaatc cttgtctttt tttgctcaac aagccacatt
     4021 tcaacctttt caaacagttg taatgttatt accaaaaaca gggtggctat cactcctaaa
     4081 aaataaaaac cggcaccaaa aaccatgcca atgcctgatg ttgtccatat tgtagcggca
     4141 gtggtaagcc ccttaatgtt gaatccatat ttaaaaatgg ctcctgcacc taaaaagcca
     4201 atgccgctta taacctgagc cgacagtcgt gcaggatcag cgttttgcgg tgaataagca
     4261 taaggaataa aatatgacag gatcatgaca gtacatgcgc ccagtgacaa taccatatgt
     4321 gtgcgcaatc ctgccggctg ataatttcgt tcacgttcaa agccaataat aaaacctgca
     4381 accagtgcac agagtattct catcaaaact gtaaatgggt gtactatatc taatcctgtt
     4441 acactgtcaa taaccacatt catgcagttt ttcccggatt accatgattt gatgttcaac
     4501 actttttagc gaggtactgc catacgattg ctttcgttct gttgattttt caggattgat
     4561 ataatcataa atatcattgt caaattgctc agaaaacttt tttaatgctt caagtggcaa
     4621 tgtaaagaaa tccacattat gttgttcaca atatttaact attgaaccta ctatctcatg
     4681 cgcctgtcta aaaggtgtgc ctttcatgac cagataatct gcaatatcgg tagctgtaga
     4741 aaaattttta tagattgcct tacgtgctac ttctccatta acatgcatgg tggttatcac
     4801 ctcattcata ccgctggcaa taagctttac cgtatcaatg gcatcaaaca atggctcctt
     4861 gtcctcctgc aaatcgcggt tataggtcat gggcaaaccc tttagtagcg taagcagagt
     4921 aatcaaattg ccatacaacc tgcctgtttt tccacgaacc agctcagcaa catcaggatt
     4981 gcgtttctga ggcattattg aagagccggt agtaacgctg tcagataatg ttataaaatt
     5041 aaactcagca gttgaccaca aaatgatgtc ttccgctaac cgtgaacaat gcatccccat
     5101 tattgatgaa aaatataaaa aatcagcaat aaaatcacga ttgcttactg catccattga
     5161 attatgagcg atagctccga aatgaagctc atctgccagg aactgtcggt cagtagtata
     5221 attaacccct gccagcgctc cgctccccag aggaagttca tccgtttgca cataagcaaa
     5281 ttcaagccgc tgtacatcgc gaagcattgc ccatgcatat gccagcagat gatgagccat
     5341 cctgacaggt tgtgcaatct gcaaatgtgt atatcccggc ataacaatat gttttgtttc
     5401 ttctgctttt tgtacgatag taactattaa ttgaattaac agttttttta tttcatacat
     5461 ctcatcttta agatataacc gcacatcagt gatgacctgg tcattccgtg agcgtgccgt
     5521 atgcaaacgt tttcccgcat caccaatgag ctcagtcagg cgtgcttcaa tattcatgtg
     5581 tatatcttca agcgtttcat tgaatgggaa ttttccgttt tcaatctcct gttcaatgat
     5641 ggaaagcccc ttttgtatgt catcacaatc tttttgtgat ataatttttt gcttgcataa
     5701 catttttgca tgggctaatg agccacgaat gtcatgttta tataatctca tatcatagtg
     5761 gagcgatgtt gcaatttcct ctgctgcttt tgatggtgca ttagaaaaac gcccgcccca
     5821 taatttcttt tttcccaacg cattactcct caaannnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnattaga aaaacgcccg ccccataatt tcttttttcc caacgcatta
     6001 ctcctcaaag aaagataaaa ttattcatca atattactat gaatagaaaa gcaaccaaag
     6061 aatttctcca ccattttgtc attctgagga acgtagtttt taagaatcta ttcgtttcat
     6121 aaaacaccga gattcttcac tttgttcaga atgacatgcc atgtttccag aataacatac
     6181 tatctcagtg ccataccagg tttgatttca aaatctgctc gttaacttac aatttttata
     6241 gtatactatc acccatacca gggttttaat aattgggatt attcattgct ataatagtgc
     6301 acccatatag gggtagcctt tatgatttta tattttgcaa tagaatatcg atgtaatgat
     6361 caatttcgtt aatcagtggt ttcaactcat attccagtat atcagtaagg ctcaccacat
     6421 cattgttttc cataacggag acaatctcag taagcatgtc ctgaattttt atattaagct
     6481 cttccagcga ttgtccatta tatataaatt cggcaatatt gatattaaat accggtgaaa
     6541 cctgataaca tgttcgcatg taaagatata aaaaatcaac aagtgtatgg ataatttcaa
     6601 gtgcttcgct atcattgcct ttttgatata atccggcaat ttcttctaaa tttttaacct
     6661 gctttcctac cattccttta aggtcaacca ttgtttgaaa aagtacatcc ggggaatcaa
     6721 cactctgtac taccagactt tttaaagcat cactgcatag caaaactctg aaaagcgtca
     6781 tggtatcatg taaaatattg ttagctgtgg taagcaaatt tatatcggtg ctactggaaa
     6841 tgttatgtcc aaatgaatat actgacccaa tgaattcctg caccgtcata tccttaaatc
     6901 gtaaatcatc cctttctaaa cccagcatgt gcaacaccga ttgtaatacc gatacaatcc
     6961 attcaaaacc attttgcaac tgaagcaatt catcggcgga caatgatgta ttgcttttat
     7021 tttctaaatg ctgacatgcc ctattacagt atgcaatacc ttcttttata gagtcgataa
     7081 caatatcact caccgactgt acataacaat ttatattatt aactttttct atggaaatat
     7141 ccggtgcatt atccagttga tatactgtcc catcaactat cgctcccgaa aaaacaaggc
     7201 ttcgttctga tgcccaattg agtattgaac ccattatatc tttaactgtg gtttcttttt
     7261 ctacagcaaa atcaacagga tgatcattaa ttaaaagttg catagtgtac ctcatgtcac
     7321 ttattatcat tgtctttggg tagatttttc atctgcatat caagccagtt actttgtgct
     7381 tttgatcccg atatggcttt ttccatagaa gcaaattttt gtcgtaattt ttgttcatac
     7441 gtttttagat gctcctgctt ccgttcaatt ctattattgg tagctttaat tgacgcatcc
     7501 tctaaatcca tctttgaaac aataatattt ttccccgcac ttacatacgg atcaagaata
     7561 ctttcaatgc tgtaagcaaa tccattatca atacgattat ccccatcatt atccgaacca
     7621 aaaaattcct gaacaccttc gggattctct gataaaattt caacaagctt tgcttcatca
     7681 ataatgagtt taccttcttt aattgtttcc cattttgcat taatttcacc tgtagaaaca
     7741 cccatctgat ataacatctt aacaggcttt tcaacatttg atggatatgc ttcagttact
     7801 gcagttttca acgcgttttg caatcgcatt aacgacatat cacccataaa aattccattt
     7861 ttatatttat ccttctgata atcgcctaat ttatcacttt taacagcctt tgtcagttca
     7921 ttgttaaatg ttaaataatc attatacgct tccacaaatt ttttaatttt ttcaattgct
     7981 gcttcggtat tttgtttgat agttattgtt aatggtttgg tcgatggttt cattaaattg
     8041 attgttacac cttttagcac atcatctatg ccggaatttt tttgccgtac tatctctatg
     8101 ccatttaatt ttaattttgc atcatcggcc tgagtgatga cattttttgg ctcaagaata
     8161 ccttcacctt tgatttcagt aactaacgct gcatcctgaa aaattgcctt gccatcattg
     8221 caataaaata atatctttgt tatcttcttt tctccaaagt cctgtcctat tggtatttct
     8281 tgtttgcctt gtttgtttgg atcaattgga tatatcctct cagagcgttt cccatcttca
     8341 acggaaataa ctcctacacc tattatatcg gcaatttctt ttttttgttt tttctcaagc
     8401 ggtctttcgc gtgatacatt atatccttta agttctatcc ctttaataac agtagtttca
     8461 tcaggcccaa cttctaaccg gtacggtata acttcttctt ctgatttttc ttttgaatag
     8521 gttacattga cttctaatac agattgtttt tttgtctgat attcaacagg aaggatgtac
     8581 tcgcgccaca atttagcatc aataataact gatttacctt ctttatcaac cgataaaacg
     8641 ccatcttcat cttcaattgg tttttcgcca gtataagccg taaaatactt ggtatcaaaa
     8701 ataatttttt gaaattcttt ttctgcacct ttttcgcctt ttaccaatcc tatctttttt
     8761 aaatactctt cgtcacctct tagttttaac tcaccttttt tccctgatat cttagattca
     8821 atgcttaaaa tacctttatc atcaaccgtt ctgattgttg ttgccgatac aacacttgaa
     8881 gcaatttcat ttatcttatc actcaacgat tttaaattac caccacggaa tttgatagta
     8941 tatgaattac catcaacttc tatactgaaa gtcccgggtg gcaagatttc atcttcttta
     9001 atctggtcag ttgatatttt ctgggttgat gctaaattaa caacttccag cgtatgagtt
     9061 ccattttcag caaaccgtga agcctgtgcc tgcagcacct caggatccga aatgcgtaca
     9121 cccttttcct taaaagggga tctaaatcca tataaatctt tggttgcagt attcaaggtg
     9181 gtaagcttgg tttgcaattg gcgcaaagct tccttacgga gggagtatcg gtctttttct
     9241 tcctgccact gcagtattgg ccttgcttct acctcaacca gcttttgaat aatctggtca
     9301 gtatccatcc ctgatgctac gccgcctaaa gtgactggca tacatgtatc ccttcatgta
     9361 agataatagt aaagatatat ccatacccat actatttcaa tacattatcg gcaaaaatca
     9421 tcaatcacta aagtgacata ttatggtaat actatcttga ttcatctaca aacatgccta
     9481 tcatatcttt aatctgagca gctaatctga tcatttcctt gggtggaatc tctctgatta
     9541 tttcgttgtt atctacatct actacacgca taactactct gtcggttttt tcatccaccg
     9601 aaaaggctat acggttattt ataacttttg ctgtagcatt gagcatttcc acagcatcat
     9661 ctaaattttc ccgtgtaaat ttagattctt cgtatagcct tttctcttca tttttatact
     9721 cttgctgctt caccttaaag gtgttaacag acatattacc tgtaacttta cttatatcca
     9781 tagttaatac ctccttgtag gtacatgata acatccttgt gaaaaattgt tgccctctcc
     9841 tcttttatgg agagggcaaa atccggctgg cactgcgaaa accgggtaat accctcatcc
     9901 caataaatgc aatactaact gtggcttaaa attagcctga gccagcatcg caacaccact
     9961 ctggacaagg atctggtcct ttgtaaactc aaccatctca gcagccatat ccgcatccct
    10021 gatacgggca tcggcagcta acatattttc ataggacatt gccagagcat agatggtatt
    10081 ttccatcctg ttgtagtagc caccaaggtc tgccctttgt cgattcagct tatccagtgc
    10141 tgcatcgata taccctatca tggcgtttgc ctgaccaaca gtggaaattg aacgctttcg
    10201 attaccatca taaaggttta aagctttggc actcatagta gtaatataag ctctaatcct
    10261 ttgtccctgg tttggtccta catggaagaa tatacttcct gttcgcgaat ttcgtgcata
    10321 aatgcctgtt agcatcttca tccgattgaa ttctgctgat gttgaaatcc tgtcgatttc
    10381 gtctatcagt tgtgaaacct caacctgaat ttgtgcacgg tcggaatttg aataaatacc
    10441 atgtgcggcc tgtacagaaa gcatcctgat cctttgcaga atatcattaa tctgctggag
    10501 agacccttca gcaacctgaa taaacgaaag cccattttca gcatttcgtc cagcctggaa
    10561 caaaccatta atctgtgtcc tcatcttttc agacacagca agccctgaag catcatcccc
    10621 agccctgtta atctgctggc cggatgctaa ccgctcaatg gatttatcca ttcgcgatga
    10681 tacgatctta agctgcctgt tagcaaatat agcgcttaag ttgtgattga ttctcataga
    10741 ctctccctcc cttgaacttt aacaggatat cccactgttt atgttgagat atctcaggtt
    10801 tttaaacatg ttcatcatga acatgtatgg tcatttttag accaggggtt tactccccat
    10861 gttgcccgga ctgcccatca tccagacatc atcggccacc atatctctgg cagccggtga
    10921 cgccctccct tcatcaccgg ccgccagaac aagcagccga attgaaagga cagccccggg
    10981 aaaacagtcc agggagagtg agaatcatca caaatgtttt caaagaacca taacgtgtct
    11041 actaagctac acaagtaatc gtaacaccga ttggggttgt aaattagcct gtgccagcat
    11101 tgcagtgcct gactgcacaa gtaccgtatt cttggtaaaa tccaccatct cctcagccat
    11161 atcagtatcc cgtatacggg attctgatgc ctgcacattt tcataggctg tcataagccc
    11221 tttagcagta tgctcaagcc tgttgtaata agcacctaaa tctgcacgct gttttgagag
    11281 cttttgcaat gctgcatcca tgataccaat ggaagcattt gctccacctg gtgacgttaa
    11341 ggtaatagca actttaccgg ttgcatcttt catcctgaaa gcctgagcag tcattgttcc
    11401 aatgaatacc ctcacgcgtt gattagcatt aggacccatg tggaaccaca tacttgactt
    11461 tggattaact ttggagaatt caccagttaa tattttaaac ctgttgaatt ctgcctgaga
    11521 tgcaatacgg tcaacctcat caaccagggc agacacttca acctgaataa gctgacggtc
    11581 tgcattggta tagatgccat tggcagcctg aaccgccaga acccttattc tctgaattat
    11641 ctgggcagtc tgtgccagat acccttctgc agtctgcaca aacgacatac catcttcggt
    11701 attccgttct gcctgcctca aaccctgtat ctgggtgcgc atcttttcag acactgccag
    11761 tccagaagca tcatcgccag ctctatttat acgttcgcca gacgacagct tctccatgct
    11821 cctgttcaca ctccattgcg tgaacttcag agttctgttt gcattcacag cactcatgtt
    11881 gtggttgata atcattttac actcctttgc acataagatt tggcttccct gccatatatt
    11941 acatagcacc gggcagacag cgcaatgtaa tgtgttagca gcatttgtaa tgctgctatg
    12001 ttccaggctt gtatctgggc aaatacaaga ccagaaccac aaaggatcaa accttcattg
    12061 ttgatatcaa accactaccc caaaaggtgt tttcaaagaa caatgtcaga agacacttat
    12121 ccctgctcga cttttcgtca ccgtaggtag aaaacatgaa t
//
LOCUS       DAOSOX010000064        12012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5276_length_12012_cov_7.679686, whole genome shotgun sequence.
ACCESSION   DAOSOX010000064 DAOSOX010000000
VERSION     DAOSOX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12012
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5276_length_12012_cov_7.679686"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..838
                     /locus_tag="PLX22_06245"
     CDS             296..838
                     /locus_tag="PLX22_06245"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HOT19534.1"
                     /translation="MESKKIFSRTIDIVSYQDGPDSVYIKGEIKDTRLCQNYLVTGEK
                     QNPGVLHNMIITMRVSGSNLVISDIQVTMKQYPREECAEANYNIQKLNGISITKGFTK
                     AVKDAIGGTKGCSHVTNCILAMGPAAVQSYWAMKAQQPVSKEFDSGGSMSQYLIDTCH
                     VWRKDGPMVKNLAEIINKLK"
     gene            complement(846..1850)
                     /gene="thiL"
                     /locus_tag="PLX22_06250"
     CDS             complement(846..1850)
                     /gene="thiL"
                     /locus_tag="PLX22_06250"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT19535.1"
                     /translation="MKLNDIGEFGFIKRITPDTIYDDRNVLRAIGDDAAVFTVSHDEC
                     IVLTTDLLVERIHFIKETIAGYQLGYKSLAVNLSDIAAMGAKPQHAFVSIGIPIETPL
                     AYLDELYRGMKQLAQLYSVNILGGDTTSSKTDLIINVAVTGVAKKNSILYRNTAKAGD
                     AIYCTGFLGDSRAGLDFILQNKNPQHDYEKKLLHAHTLPRPHVEEGLFLSNTGAVHSC
                     IDVSDGLSSDLLHIAEESNVGFILDEKSIPISPQLQQYCDDNALNATDYALSGGEDYV
                     LVFTVDENASLQLEKNFYNTFNRAIYKIGIITPDKKYVMRKIDGSLINIKAGGWDHFV
                     "
     gene            complement(1837..2487)
                     /gene="thiE"
                     /locus_tag="PLX22_06255"
     CDS             complement(1837..2487)
                     /gene="thiE"
                     /locus_tag="PLX22_06255"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT19536.1"
                     /translation="MKRFTSSLYLVTDRGLAKKPIEILVEEAIRGGVDCVQLREKNIA
                     TKHFVALAKTVKNITIRYNVPLIINDRIDVALAVDADGIHLGQDDMPLTNARKLLPHK
                     MIIGITVSTINEAIEAQKGGADYIGASPVFATPTKTDTAAPLGLEGLQEIKDAVTIPV
                     VAIGGINLHNAEDVLLHGADCLAVVSAIVCADNPCQAAANLAAIIKKLRKSNHETE"
     gene            complement(2504..3304)
                     /gene="thiM"
                     /locus_tag="PLX22_06260"
     CDS             complement(2504..3304)
                     /gene="thiM"
                     /locus_tag="PLX22_06260"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOT19537.1"
                     /translation="MNLHEYAGETLKKIRAQKPLIHNITNYVVMNYTANALLACGASP
                     VMAHAYNEVRDMVGIADALVINIGTLEEEWIRSMIIAASEANKKGIPIILDPVGAGAT
                     KFRTTAALRILRAAKISVIRGNASEILALTDSSSQTKGVDSQHGVDEATDVAVELSLR
                     YNAVVAVTGVTDVVTDGNAAIKVSNGHSMMRYVTGTGCAATALIGTFCAVESDHLKSA
                     ASALAYFGIAGELAAKEAMTPGSFMIALLDKLYAITPVELEQYAKFAI"
     gene            complement(3419..4723)
                     /gene="thiC"
                     /locus_tag="PLX22_06265"
     CDS             complement(3419..4723)
                     /gene="thiC"
                     /locus_tag="PLX22_06265"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOT19538.1"
                     /translation="MTQLDYAKQGIITDAMKQAAAFEGMKPEELLQLMAKGWAVIPKN
                     IKRNFNVRAIGKGLLTKVNANIGTSTDHGDIDEECKKLETAVYYGADSVMDLSTGGNL
                     TAIRTMILQNANVMVGAVPIYRVAAELAANNRSILHMSHNQLFEAIEEQCQNGIDYMT
                     VHCGVTQKSMELASRQRRVMGIVSRGGSLLSAWMKHHKRENPLYEDYEKLCAIAHKYD
                     VTLSLGDGLRPGATADATDAAQIEELILLGDLVQRARDAGVQVIVEGPGHVPMEQIAA
                     NIVLQKRICDNAPFYVLGPLTTDIAPGYDHIVGAIGGAIAAYAGADFLCYVTPAEHLC
                     LPSVDDVRLGVIGTRIAAHSADIAKGIPKALQQDLEMSQFRKSLNWEGMISRAIDPDM
                     AKQRRSNSEDYNEKVCTMCGKLCAITIHNECELLHHDGDVAK"
     regulatory      complement(4794..4895)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4958..5299)
                     /locus_tag="PLX22_06270"
     CDS             complement(4958..5299)
                     /locus_tag="PLX22_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOT19539.1"
                     /translation="MMKTKTLQDTLQFAQMVAGKHGWVLHPDKEFLDILVEGLTTNYN
                     RYGYFSCPCRDADGIREKDKDIICPCEYCAPDIQEYGSCYCGLYNDPLFIKNGGYPES
                     IPERRQFKNFI"
     gene            complement(5306..5608)
                     /locus_tag="PLX22_06275"
     CDS             complement(5306..5608)
                     /locus_tag="PLX22_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOT19540.1"
                     /translation="MIENITFTYKDGLLSPGDITVYALSTCGHCKRALKFLDDNSVKY
                     KFVYVDLLPSEEKNNIKDYLAKKSNMYVAFPFLVIDDTTFFTGFIEEEWRRLLKME"
     gene            complement(5622..7085)
                     /locus_tag="PLX22_06280"
     CDS             complement(5622..7085)
                     /locus_tag="PLX22_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19541.1"
                     /translation="MKCKKCGNKIPLLIEICPYCGYEFHATHHKNVHNDNANTAEDAV
                     HDDRADDTQKILNDIKNIKNYESNNIGSIAILIISILLFFSIGYAKHPLKDTIIVIIV
                     LLLHEFGHLLGMMIFKYKNIKMFFIPFFGAAVSGLKLNKDGFNEAIISLLGPMPGILI
                     AIACMFFFKNNDNLNQIAASLIFINGFNLLPIYPLDGGRIMDIIFQRSMVAQLLFRIV
                     TSLALIYLAVFSHTLLLAFVGIIIILSIPDNYRTNKAVKALKADIPATVSIENEDKVL
                     QDNIGIIYATVKAIFPKVKGKRYANIILAVWDKLTFKAPNFIKSLIITIIYGMSIAML
                     VVSTALFYFNYSRSHSRLESSIVSMVDDSGNHYQLQQIKYHNKLLYEIQVHNDLYHGF
                     CKEYSPITNKVVTEGYWYKGKWDKEWKFYNSKGEVYRIDFFDRGTFKFRSEFINGTLQ
                     IIKFEELNNNDKMKFKNHEQAKPLGPNNVHVSTDIVQ"
     gene            complement(7157..7339)
                     /locus_tag="PLX22_06285"
     CDS             complement(7157..7339)
                     /locus_tag="PLX22_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19542.1"
                     /translation="MDELILYLISEYHTAVMHTLTIISVMAVWVVICGCIRFGIAVYR
                     RAKGIYPPAESLREGH"
     gene            7739..8425
                     /locus_tag="PLX22_06290"
     CDS             7739..8425
                     /locus_tag="PLX22_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT19543.1"
                     /translation="MSIVDNYHYIQSEIKNIALRCNRNPDDITLIAVSKTFPPEIIQE
                     AIDNGITLFGENRVQEAKAKIPQLKGNFTFHLVGHLQSNKAKDAVKLFDCIHSIDRLS
                     TAIEVDKEAKKIDTIQKILIEVNTSGEESKFGVVPSQAIALCKSILDLHNIQCHGFMT
                     VGPLTDDRSAIRNAFALLRTLRDEINIKLGTSLHELSMGMSGDYDIAIEEGSTMLRIG
                     TAIFGKRISQ"
     gene            8422..9225
                     /gene="proC"
                     /locus_tag="PLX22_06295"
     CDS             8422..9225
                     /gene="proC"
                     /locus_tag="PLX22_06295"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT19544.1"
                     /translation="MNIQKTTIGIIGLGNMGGAIGTGLSRIIDPKNIIGYDISSQAMT
                     SLTSTLSISTASSIQELSHKSDVIIIAVKPAIVASVASELKSTTKNKLIISIAAGITL
                     HKLSEWLGDSFRIIRCMPNMPALVGEGMTVLSTTSANNDDMEIAQQIFGALGRVMVVS
                     EQLMDAVTAVSGSGPAYVFTLIQAMADAGVKSGLTRDNAVTLAAQTVLGSATMVLQGE
                     EPIVLRGKVASPAGTTIDAIHVLEKHGFSGIIMDAIEAAFLKSRTLGGK"
     gene            9226..10182
                     /locus_tag="PLX22_06300"
     CDS             9226..10182
                     /locus_tag="PLX22_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOT19545.1"
                     /translation="MLTNYHVWQIKKAIKVLIPVMVFIFIAGNIICYFVKEPVKIPDD
                     AVYKKNGFLLFKDADAYITILKKYPYNPGVTLTIYTMREGESYWDVIQRYRISIDTFI
                     GANPHLDSLLAKAGTQVVIPHQDGLLIPCRHFYDAWLAKLSINGTTNIKGHYLHHPFE
                     LLAMDDIRFAFIKHAKPIAVNDKIEKLYTLRQKFQSPINGRYTSMYGDRVDPLEHGMA
                     FHNGIDICAPMGTPINPVRNGFVNFTGWLDGYGFTIIIQHDEGYESWYGHCQKIFAQK
                     GDFVTNNSVIGTVGSTGRSTGPHLHFMLLQHGKMLNPIMFIW"
     gene            complement(10193..10924)
                     /locus_tag="PLX22_06305"
     CDS             complement(10193..10924)
                     /locus_tag="PLX22_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT19546.1"
                     /translation="MRAIVLAAGTGSRLGEMTKNKTKGMVRVNGKPLIDYLFEFFELD
                     FFDEIIVVGGFAFDELNSHLSGKNIDNIKILENKDYLKGNIFTLITALKTFAHDSFLI
                     TNVDHIYPKSMFQRMKQSLYKITAMCDFDRTLGLDDMKVKLLADKKTIAHISKQLTDF
                     DCGYIGMTYVDKSMEIAYRDAAYKAIERYGEKAVVENIVQILAENPKVAPVICDLSGF
                     GWYEVDDENDLIKAEKGLLQNQNFN"
     gene            complement(10912..11550)
                     /locus_tag="PLX22_06310"
     CDS             complement(10912..11550)
                     /locus_tag="PLX22_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT19547.1"
                     /translation="MKTRALLFDFDGTLVDTMEGFADIAGEVIHQFHPAISYEEARRR
                     YLETSGVPFFQQLEIILPGDPTNSKKAAIFEETKKEGFFRSTFSEEVRFVITQLRGKG
                     ILVGVSSNNFQYLIEQFIEREGLQFDIILGFKDGFEKGKHHFEYVLQHFQLKKDELTF
                     VGDSLKDAEKAISNDISFIGLCGTFTKEQFLDKYPNIITINSLKELLDICER"
BASE COUNT         3723 a         2431 c         2039 g         3819 t
ORIGIN      
        1 taaaaattat taccatacat tgtcaaccaa ttatttatat ataacatttt tattcattta
       61 ttgatatata actacaaaac atatttatat atatataata atttttatag gaaaagcaac
      121 ttatagcttc caataacttt aaatttgttt ctcacattat agtagcgatt tcgtcgtctg
      181 tattgagtca tcaatggaca ttatgcaata actttccatg gaattttaat tgaaaaatta
      241 tttgtttttt attacatagc agtaagctta ttacttatta taggagaaag ttactatgga
      301 aagcaaaaaa attttttcta gaactattga tatagttagt tatcaagatg gacctgattc
      361 agtttatatt aaaggtgaaa tcaaagacac acgcctgtgc caaaattatc tggtaactgg
      421 agaaaagcaa aacccgggtg tactgcacaa catgatcatt acaatgcgtg tcagcggcag
      481 caacctggtg atcagcgata tacaggtaac aatgaaacag tatccacgcg aggaatgtgc
      541 agaagcaaat tataatattc aaaaattaaa cgggatttct ataacaaagg gatttacaaa
      601 ggctgtaaaa gatgctattg gcggtacaaa aggttgttcg catgtaacaa attgtattct
      661 tgctatgggg cctgccgctg ttcagagtta ctgggctatg aaagctcaac aaccagtttc
      721 aaaagaattt gatagcggcg gctcaatgtc tcaatatttg atagatacct gccatgtatg
      781 gcggaaagat ggtcctatgg taaaaaattt agctgagata atcaataaat taaaataata
      841 ttttattata caaagtggtc ccatccacct gctttgatat taattagtga accatctatt
      901 tttctcatta catatttttt atctggtgtt attattccta ttttgtagat tgctctatta
      961 aatgtattat aaaaattttt ttctaattgc aatgaagcat tttcatcaac agtgaaaacc
     1021 agcacataat cttcgccgcc cgacaatgca tagtcagtag cgtttaatgc attatcatcg
     1081 caatactgtt gcaattgggg tgaaattggt attgattttt cgtccagtat aaatcccaca
     1141 ttactttcct ctgcaatgtg caatagatca gagcttaacc catcacttac atctatacag
     1201 gaatggactg cacccgtatt gcttaaaaac aaaccctctt caacatgagg tcgcggtaat
     1261 gtatgagcat ggagcaactt tttttcatag tcatgctgtg gatttttatt ttgtaaaata
     1321 aaatccaatc ccgccctgct atcgcccaga aagcctgtac aatagatagc atcaccagct
     1381 tttgcggtat tacgatataa tatgctattt ttttttgcaa ctcctgtaac tgcaacatta
     1441 atgattaaat cagtttttga agatgttgtg tccccaccca gtatattgac actatacagt
     1501 tgagctaatt gtttcatccc cctgtatagt tcatccagat acgcaagcgg tgtttctatt
     1561 gggattccta tgcttacaaa ggcatgctga ggtttagctc ccatagctgc aatatcactc
     1621 aggttaaccg ccagcgattt gtagcccagc tggtaacccg caatggtttc ttttataaag
     1681 tgaattcttt ccacaagcaa atcagtggta agcactatac attcatcgtg actcacagta
     1741 aatacggctg catcgtcacc tatagcgcgc aatacatttc tatcatcata aatggtgtcg
     1801 ggggttatcc gcttaataaa accaaattca ccgatatcat tcagtttcat gatttgattt
     1861 ccttaacttt tttatgattg ctgcaagatt ggctgctgcc tggcagggat tatccgcaca
     1921 aacaatggct gaaacaacag ctaaacagtc agctccatgg agtaaaacat cttcagcatt
     1981 gtgaagattt ataccgccaa tggcaacaac gggtatcgtt acagcatctt ttatttcctg
     2041 caatccttcc aatcctaacg gcgctgcggt atcagtttta gttggggtgg caaacacagg
     2101 gcttgcacct atataatcag ctccaccttt ttgtgcttca attgcctcat taattgtaga
     2161 aaccgttatg ccaatgatca ttttatgagg aagcaatttt cgagcatttg tcaatggcat
     2221 gtcatcctgc cctaaatgga taccatcagc atctactgcc agcgccacat ctatacgatc
     2281 attaattatt aaaggaacat tatatctgat agtaatattt ttaacggttt ttgccagtgc
     2341 aacaaaatgt tttgtagcaa tatttttttc ccgcaactgt acgcaatcca ctcctccccg
     2401 tatagcttct tcaacaagta tttcaatagg cttttttgcc aatcctctgt cggtcactaa
     2461 atataatgaa gatgtaaaac gtttcattgt aattacctca tacttatata gcaaattttg
     2521 catattgctc caactctaca ggagttattg catacagttt atccagtaaa gcaatcataa
     2581 agctgcccgg cgtcattgct tcttttgctg ccagttcacc agcaatacca aaatacgcca
     2641 gtgccgatgc agccgatttt aaatggtcac tttcaactgc acaaaatgtt ccaatcaatg
     2701 ctgtcgcggc acagcccgtt cctgttacat aacgcatcat tgaatggcca ttgcttactt
     2761 ttattgctgc atttccatca gtgacaacat cagtaactcc tgttactgca acaactgcgt
     2821 tgtagcgtaa cgatagttcc actgcaacat cagttgcttc atcaactcca tgttgtgaat
     2881 caacaccttt tgtttgtgag ctactatcgg tcaaagctaa aatctcagaa gcattaccac
     2941 gaataacaga aattttagct gcgcgtaaaa tcctcaatgc agctgtagtt cggaattttg
     3001 ttgcacctgc tccaacgggg tcaagaatga ttgggattcc ctttttattt gcttcgctgg
     3061 cagcaataat catagaacga atccattctt cttcaagggt gccaatatta ataacaagag
     3121 catctgcaat accaaccata tcacgtacct cattataggc atgtgccata accggggaag
     3181 cgccacaagc taacagggca tttgcagtat aattcataac cacataattg gtaatattat
     3241 gaatgagtgg tttttgtgct ctgatttttt ttaaggtttc tccggcatat tcatgtagat
     3301 tcattgtgtt actccaaaaa aatagtatag tactgttagc aactgaagaa tctatgcttt
     3361 accataacga cagattcttc acttcgttca gaatgacatg ctgcactaag attcttcact
     3421 atttcgcaac atctccatca tgatgcaata attcacattc attgtgtatg gtaatagcac
     3481 agagcttacc gcacattgtg catacctttt cattataatc ttcactgtta ctgcgccgtt
     3541 gttttgccat atccgggtca attgccctgc ttatcatccc ttcccagttt aaggattttc
     3601 tgaattgtga catttccaaa tcctgctgca gcgctttggg tatgcccttt gcaatatcag
     3661 ccgaatgagc tgcaatacgc gttccaatta ctcccagccg cacatcatca accgatggaa
     3721 ggcatagatg ctcagcaggg gtaacataac acaaaaaatc agcgccagca taggcagcga
     3781 tagctcctcc aattgctccc acaatatgat catatcccgg ggcaatatcg gttgtcagcg
     3841 gtcccagcac atagaatgga gcattatcac agattctttt ttgtaacaca atgtttgccg
     3901 ctatctgttc cattggaaca tgccccggtc cctcaactat cacctgcact cctgcatcac
     3961 gggctctttg tacaaggtca cctaataaaa taagttcttc tatctgagca gcatctgttg
     4021 catcggctgt tgcacccggg cgcagtccat cacctaaact aagcgtgaca tcatacttat
     4081 gagctatcgc acataatttt tcatagtcct cataaagtgg attttccctt ttatgatgct
     4141 tcatccatgc tgaaagcaat gagcctccac ggcttactat gcccataacc cttctttgcc
     4201 tggatgccaa ttccatactt ttttgcgtaa ctccacaatg gacagtcatg taatcaatac
     4261 cattttgaca ttgctcttca attgcttcaa aaagctgatt gtgcgacatg tggagtattg
     4321 acctattgtt agcggcaagc tcagcagcaa cccggtaaat aggtactgca ccaaccataa
     4381 catttgcatt ttgtagtatc attgtacgta ttgctgttag attgccgccg gtcgaaagat
     4441 ccataacact atcagcacca taatacaccg ctgtttccaa ttttttgcat tcttcatcaa
     4501 tatcaccatg gtcggttgaa gttccaatgt tagcattaac cttggtaagc aatcccttcc
     4561 ctattgctcg tacattgaaa ttccgcttta tattttttgg aataacagcc cacccctttg
     4621 ccattaattg caaaagttct tcgggtttca taccttcaaa tgctgctgcc tgtttcatgg
     4681 cgtcagttat gataccctgt tttgcgtaat ctaattgtgt catacaggta acccccacaa
     4741 caaaaaaacc gctcagcgat tgagcggttt ttttacatta atactattat aaaagcattg
     4801 tacaaactgc tccctccgct ggcattaccc agttcaggtt caacgggtat actctcaggc
     4861 ctgtgtatcc acacaaacca cccctgcttt taagaacatc atacttagta ctacaatact
     4921 atgaacacat gaaaaatcaa gagaaatatt acaataatta aataaaattt ttaaactggc
     4981 ggcgttcagg gatgctttcc gggtaaccac cattttttat aaatagcgga tcattatata
     5041 agccacagta acagctgccg tattcctgta tatcaggtgc gcagtattcg cagggacata
     5101 taatatcctt atccttttct cgtatgccgt cagcgtcccg gcatgggcat gaaaaatagc
     5161 catatcgatt ataatttgtt gttaatcctt caaccagaat atcaagaaat tccttatcag
     5221 gatgcaatac ccagccatgc ttgccagcaa ccatctgagc aaattgcagg gtgtcctgta
     5281 atgtttttgt tttcatcatt gtttactact ccatttttaa taatcgtcgc cattcttctt
     5341 caataaaacc cgtgaaaaat gtggtatcat caataactaa aaacggaaat gcaacataca
     5401 tgtttgattt ttttgccaga tagtccttta tattgttctt ttcctctgat ggcaatagat
     5461 caacataaac aaatttatac tttacagaat tgtcatcaag aaactttaat gcacgtttac
     5521 aatgtccaca ggttgataac gcatacacgg ttatatcccc tggagaaagt aaaccatcct
     5581 tataggtaaa ggtaatattt tcaatcatat caatcctccc attactgtac tatatcagta
     5641 cttacatgaa cattgttagg tccaaggggt tttgcctgtt catgattttt gaatttcatt
     5701 ttatcgttat tattgagttc ttcaaatttt ataatttgca aggtaccatt aataaattca
     5761 ctacgaaatt tgaatgttcc cctgtcaaaa aaatcaatcc tgtaaacttc acctttacta
     5821 ttataaaatt tccactcttt atcccattta cccttatacc aataaccctc ggttacaact
     5881 ttatttgtta ttggcgaata ttccttacaa aacccatgat aaagatcatt atgtacctga
     5941 atttcgtaca gtaatttgtt gtgatactta atctgctgca attgataatg attgccgcta
     6001 tcatcaacca tagaaacaat actgctttcc aatcggctat gactacggct atagttaaaa
     6061 taaaataatg cagtggatac tactaacatt gcaattgaca taccatatat aatagtaata
     6121 atcaaacttt taataaaatt tggtgcttta aaagttagct tatcccatac cgcaagtatt
     6181 atattggcat atcgcttgcc cttaaccttg gggaaaatag ctttaactgt tgcatatatt
     6241 ataccaatgt tgtcttgcaa cactttatcc tcattttcaa tggacaccgt agctggtata
     6301 tcagccttca aagcttttac tgctttattg gtacgatagt tatcgggaat cgatagaata
     6361 atgataatcc caacaaatgc caataacaat gtatgggaaa aaactgctaa ataaataagt
     6421 gccagcgatg ttactattct aaataataat tgagctacca ttgacctttg aaaaatgata
     6481 tccattatcc tgccaccatc aagagggtaa ataggcagta agttaaaccc gttaataaat
     6541 ataagcgatg ctgctatttg attaagatta tcattatttt taaaaaaaaa catacatgct
     6601 atggcaatca atatgccagg catgggacca agtaatgata tgatagcttc attaaaacca
     6661 tctttattta attttaaacc acttacagct gcaccaaaaa aaggtataaa gaacatcttt
     6721 atatttttat atttaaaaat catcatgcca agtaaatgtc caaactcgtg tagcaacaaa
     6781 acaataataa cgataatggt atcttttaaa ggatgtttgg cataaccaat agaaaaaaac
     6841 aaaaggatgg aaatgattaa aatcgcaata ctacctatgt tattgctttc ataattttta
     6901 atatttttaa tatcatttaa aattttttga gtatcatctg ctctatcatc atgcacagca
     6961 tcttcagctg tatttgcatt gtcattgtgc acatttttat gatgagttgc atggaattca
     7021 tatccacaat aagggcatat ttcaatcaac aatggtatct tgtttccgca ctttttacat
     7081 ttcataatgt ttttgcctta taaaaaaagc gatgacagtg catcgctttt tataaattag
     7141 tataaaaact attgaattaa tgcccttctc gcaagctttc agcgggtgga taaatgccct
     7201 ttgcccttct ataaacggct ataccaaaac ggatacatcc acagatgacc acccacactg
     7261 ccataacaga aatgatagta agagtatgca ttaccgctgt atgatattca ctaatcagat
     7321 atagaattaa ttcatccatc actcacctca aaaatgaaag tttattatga taagtacaat
     7381 aataactttt aaattttcat ttaatattgt caatacaaaa ttttataatt cataaaattt
     7441 taccggaaaa tgttgttttt ataaaattga aagtgcgttg cttttcatta aaggctttac
     7501 attttttttg taaaatgata gtgtacaatc actattttct ttttgatgaa ggtattgcat
     7561 tttgttaaaa agtgtttgac tttataaatt accatacaaa taattaatat taatgagtaa
     7621 atagccgttt ttttatgatt gtatcatcag gtgcagcaaa aatatcatac aaaacaaagc
     7681 atcaatcatt gaaatagcta tagctactat ttaaaaaagg ttttgccaat ttaacgctat
     7741 gagcatcgtt gacaactatc attacatcca gtctgaaata aaaaatattg cccttcggtg
     7801 caacaggaac cctgatgata ttacacttat agctgtaagc aaaacattcc cccctgaaat
     7861 tatccaggaa gctatcgata atggcattac tttatttggc gaaaacaggg tgcaggaagc
     7921 aaaagcaaag attccgcaat taaagggtaa ttttacattt caccttgttg gacatcttca
     7981 gtcaaacaag gcaaaagacg ccgtaaaact gtttgattgc attcattcca ttgacaggct
     8041 atcaaccgca attgaggttg acaaagaagc aaaaaagatt gatacaatcc agaagatttt
     8101 aattgaggtt aacacctcgg gtgaagaatc aaaatttggc gttgttccgt cacaagccat
     8161 tgcattatgc aaatctattc tggatttaca caatatccaa tgtcatggtt tcatgacggt
     8221 aggtcctttg accgatgacc gttcagcaat acggaatgca tttgcattat tgagaacatt
     8281 acgggatgaa ataaatataa agcttggaac ttcattacat gagctatcga tggggatgtc
     8341 aggtgattac gatatagcaa ttgaagaagg ctctaccatg cttagaattg gaacagcaat
     8401 ttttggtaaa aggatatcgc aatgaatata caaaaaacaa caatcggtat cattggtctg
     8461 gggaatatgg gcggtgctat cggtacaggg ctttccagaa tcattgatcc aaaaaatatt
     8521 attggttacg atatcagcag tcaggcaatg acttcactta ccagcacact atcaataagt
     8581 actgccagct caattcagga gctatcgcac aaaagtgacg ttatcatcat tgccgtaaaa
     8641 cctgcaattg ttgcgtcggt agccagcgag ctaaagagta caaccaaaaa caagcttatc
     8701 atttcaatag ctgccggtat cacacttcac aagctttccg aatggctggg cgatagtttc
     8761 cgtataatca ggtgcatgcc caatatgcct gcccttgttg gcgaagggat gaccgtacta
     8821 tcaacgacat ccgccaataa cgatgatatg gagattgcac agcaaatctt tggagcgctg
     8881 ggcagagtca tggtagtttc cgagcagttg atggatgctg ttactgctgt ttcgggaagc
     8941 ggtccggcat atgttttcac attgatacag gcaatggcgg atgcgggggt taaatcagga
     9001 cttacacgcg ataacgccgt gacactggca gcccaaacgg tgttaggttc agctacgatg
     9061 gtattgcagg gcgaagagcc aattgtttta cgggggaagg tagcttcccc ggccggcact
     9121 accattgatg cgatacatgt tcttgaaaaa catggatttt ccggaattat aatggatgcc
     9181 attgaagctg catttctgaa atccagaacg cttggaggta agtaaatgct tactaactat
     9241 catgtatggc agataaaaaa agctataaaa gtacttatcc ctgttatggt ttttattttt
     9301 attgctggca atattatatg ctattttgta aaagaaccag taaaaatacc cgatgatgcg
     9361 gtttataaaa aaaatggctt tttactcttt aaagatgcag acgcatacat cacaattcta
     9421 aaaaaatacc cctataatcc cggtgtaaca ttgaccattt atacaatgcg tgaaggtgaa
     9481 tcatactggg atgtcatcca gcgctataga ataagcattg atacatttat tggtgccaac
     9541 ccgcatctgg attcattact ggcaaaggca gggacacagg ttgttattcc tcatcaggat
     9601 ggactgctca tcccctgcag gcatttctat gatgcatggc tggcaaaact gtctataaat
     9661 ggtacaacaa acattaaggg gcactatcta caccacccct ttgagcttct ggcaatggat
     9721 gatatacgtt ttgcctttat aaaacatgcg aagccaattg ctgttaatga taaaatagaa
     9781 aaattatata ccttacggca aaaattccaa tcccccatca atggccgata cacatcaatg
     9841 tacggcgata gggttgaccc attagagcat ggcatggcat tccacaatgg cattgatatc
     9901 tgcgcaccga tgggaacacc aataaaccct gtaaggaatg gatttgtaaa ttttaccggc
     9961 tggcttgatg gttatggttt taccatcatc attcaacatg atgaaggata cgaaagctgg
    10021 tacgggcact gccaaaaaat atttgcgcaa aaaggtgatt ttgtgacaaa caattcagtc
    10081 attggtacgg ttggctctac aggacggtcc accggtcctc acctgcattt tatgctgtta
    10141 cagcatggta aaatgctcaa tccaataatg tttatatggt agatagttac tatcaattaa
    10201 aattttgatt ttgtaaaagt cctttttcag cttttatgag atcattttcg tcatccacct
    10261 cgtaccagcc aaaacctgat aaatcacaga ttacaggagc aacttttgga ttttcagcca
    10321 atatctggac aatattttca accacggctt tttcgccata ccgctcaatt gctttgtacg
    10381 cagcatctct ataagctatt tccatacttt tatcaacata ggtcatacca atataaccgc
    10441 aatcaaaatc agtaagctgt ttactgatgt gggcaatagt ttttttgtca gcaagcagct
    10501 ttactttcat gtcatccaat cccaatgtac gatcaaaatc acacattgca gtaattttat
    10561 aaagcgattg tttcatccgt tgaaacattg attttgggta gatgtgatca acattggtga
    10621 ttaaaaagga atcatgagca aatgttttta atgcagtaat caatgtaaag atatttcctt
    10681 taaggtaatc tttattttca agaattttta tattatcaat attttttcct gaaaggtggc
    10741 tattaagttc atcaaatgca aaaccgccta caactataat ttcatcaaaa aaatccaatt
    10801 caaaaaattc aaatagataa tctataagcg gtttaccatt gacacgcacc atgcctttgg
    10861 ttttattttt tgtcatctcg cccagcctgc tgccagttcc tgctgccaga actatcgctc
    10921 gcatatatct aacaactcct ttaatgaatt gatggtaatt atattgggat acttatctaa
    10981 aaattgctcc tttgtaaatg tgccgcataa acctataaat gaaatgtcat tgcttatagc
    11041 cttttctgca tctttaaggg aatctccaac aaatgtcaat tcatcttttt ttaattgaaa
    11101 atgttgcagt acatattcaa aatggtgctt acccttttca aatccatcct taaatccaag
    11161 gattatatca aattgcagtc cttctctttc tataaactgt tcaataagat actgaaaatt
    11221 atttgatgaa acaccaacca gtataccttt tccccgtaat tgtgtaataa caaaccgcac
    11281 ttcctcagaa aatgttgatc tgaaaaagcc ctctttcttt gtttcctcaa atatagctgc
    11341 ttttttgctg tttgttggat cacccggtaa aattatttcc agttgttgaa aaaatggaac
    11401 gcctgaggtt tctaaatatc tacgtcgcgc ctcttcatag gaaattgcag gatggaattg
    11461 gtgtattact tcaccggcaa tatcagcaaa tccttccata gtatcaacaa gtgttccatc
    11521 aaaatcaaaa agcaatgctc ttgttttcat acatgtatca ccattcaagt tttcatcaat
    11581 cttatggata atcgctactt gtcaatataa aaaattaact gcaggcgcac cacggtattt
    11641 cccgcctgtc ctgacaacga agctgtgtaa tggttgtgtg cggcaggctg accaatcatg
    11701 cgcctgcatc caaatgcgcc gtgcatttca caatacttaa ccccccttgt gttccccccc
    11761 tttataaaag gtgggaattg tgtggatggt ttattagcac aaaataggtt tgtattcttc
    11821 ataattgttt ttgcagctta atcccgtagt aaccacatta tgcttacttg ataagttctg
    11881 cattcttcat ggtaaaattt cttcaaatct tgtagggatg gaattgatat ttaataaatg
    11941 gggttggaat ttttttttca tattttcttt tgttgggcga caaaagtttt atcgtttcgt
    12001 aaggggatat tt
//
LOCUS       DAOSOX010000065        11963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5299_length_11963_cov_8.658717, whole genome shotgun sequence.
ACCESSION   DAOSOX010000065 DAOSOX010000000
VERSION     DAOSOX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11963
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5299_length_11963_cov_8.658717"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1084)
                     /locus_tag="PLX22_06315"
     CDS             complement(<1..>1084)
                     /locus_tag="PLX22_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT19548.1"
                     /translation="NRLKESGFNNAFTLPPETPYEQYVELMTKVIAAARLETDNKEAI
                     VAMNSPFIYKPAERPVNNIYSKGILLIHGLSDSPYFLQAIGKYFASKGFLVYGLLLPG
                     HGTVPGDLLKVTYDEWIKATHYGMLQLKKNAQEVYMGGFSTGATLSVNYVLSNTNHDV
                     KGLFLFAPAFAINSSVAWLAPIVSTFKDWTDIDDDVDYTKYQSLTLNGAAQTYKLIKK
                     VDSYFEEGKTISVPVFVVQSIDDETVKAERTLYVMKNYVTGKKMFVLYTTQPEKDYDG
                     EGNFVVTVNSFLPKEKILNFPHICVTIPPDDPHYGRNGDYRNCLYYKQGSPERNKCLT
                     DPNIWKGETTAENIEKYKVIARLTYNP"
     assembly_gap    1085..1184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1660..2073)
                     /locus_tag="PLX22_06320"
     CDS             complement(1660..2073)
                     /locus_tag="PLX22_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19549.1"
                     /translation="MKRVGFGFLIVLIFYNATTAFSKEVPFTLEDRDRIIRLEAKLDE
                     IDKRFEQIDKRFEQIDKRFEQVISFLWILTTIFIAITSATIGFAFWDRKTSLKPIEEK
                     VTINEKKLEHALAALRKLASVDKKVHAILKEFNLL"
     gene            complement(2228..3376)
                     /locus_tag="PLX22_06325"
     CDS             complement(2228..3376)
                     /locus_tag="PLX22_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOT19550.1"
                     /translation="MAVLPDAPIGVMGIGRIGKLLVWLYAAKKQQKEIVIATGRKTGT
                     SLEDLATYFEYDSTYGSFNRFIGGFSGKGAVEIKDGHVYLNGVKLIWLNEPDYRVPGK
                     IPWDTFGVNVVFDTTGKMLDPTRNDENSLRGHLNHAKKVIVTAPFKVKDKGKQIPDDA
                     VTVVGGVNFDKYDAGKHVIISNASCTTNCCAPPIKALVDHFGDKFISYALTTVHAVTN
                     SQSTLDVLPKDGDKDTRKRRSILNNMIPTSTGAAKAVLEVIPEVQKLGIGSQASSIRV
                     PVNTGSIVILDVSLLGDYDNQYIHKIFKQYSEKNPDIMCYSEKQLVSSDIVGSTYSTI
                     YDAEFTHHRTTKRGDNYYTMVDLNFWYDNEFGYVSSLSRLYDLVTGRR"
     gene            complement(3555..>5738)
                     /locus_tag="PLX22_06330"
     CDS             complement(3555..>5738)
                     /locus_tag="PLX22_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOT19551.1"
                     /translation="ILLSQDVFEPVDTIEFKRHIAFGIPSMYGSYKEKKFDTLKVFFH
                     CNIVRVLLFEKIIENISIYPHQKIDYDAIKRVVELFIQSFEIDGLANHEMRSVTSLLD
                     APNITVTQFRDIVNSLLVIHGEISDRFNDTFKSVSRIAIKNIGMDNIIPDFIPPDQPA
                     NVEVIVDRFLRHRVMQSPLLQLMDNLLLKLRDHLNSVYSYMGNIVVLGAIDARMKKGK
                     LIHVIGKYEGTYDETELYVPLWEVGAKAQGLIIAANMDGINVPEGIVISSELYKRIKD
                     GNINNPRFKRKLIYMLKKYIDAFTGFRFANPQNPILLSVRSGAVFSMPGVMDTITNVG
                     MTEEIVQYFTQFDEWFAWDCYRRLIHDFAISAYGMDRHIFENLMTQAKDEAGVDLKEK
                     LNGKQMSMLTLKYRYAINKAGHSAPKDPYEQLFYAIIAVFKSWDSPIARNYRQFINIS
                     DDWGTAVIAQRMVFGNLSPTSITGVVHSQYIEYEELQIVGEYKTRAQGHDIVSGVAKV
                     FPINEQQKLKFARFAMYPSLEKAYPNHYATLRDAVSRLRKRWGNDIEVEFTFENDVLY
                     ILQIRGMAKHMFDIEELVETPQQLSQYLLGQGLAASGGAVSGRAVFDINRIEAIRMQY
                     PKDKIILIRPETNPEDVIGLQKSDGILTSVGGMTSHAVLQMRRLEKSGVSDFSIMKIA
                     ESENIAVINREQGGKIVIREGDVITIDGNTGHVYLGAHPTRKVHR"
     assembly_gap    5739..5838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5839..7802)
                     /locus_tag="PLX22_06335"
     CDS             complement(<5839..7802)
                     /locus_tag="PLX22_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19552.1"
                     /translation="MEYEIYDVNNILLPVEHKIGALSRAKNLVAEMTHPNIDWKYMVT
                     EIRAYLYDYMYDIVPHSDRVLPVIFHYLKEATVRKRGSTIRAADTFLDRYLFLLKKEI
                     AGDSSLDNVTAIFDKEAIDFCRILLSDTQEGFYLEDVNQRIIQLMELSLQRKKLDAQL
                     FELCTDIIINQFKLYVERSIVVEEEYMDSLRNLWNSDGESIATLRQYVQTVTKEAYEK
                     KLQIALDSRTTITDYEKLLQQLINVIDFHHNETAWEKICIAARECIVRNIIQDDSVLL
                     SLLSFMVKKSQQGNDTNLQLYISRSVASLCGVLAQQQRFDLLKQVVQMVVPVLISEIE
                     RGGNYNAAFATIYTIGKTVVEINNRPIIDLFVDILVHARFCFPQFTGIASDWSVMVNA
                     SHLANIRTWLELIELNPVYMKRLAASLIANLTLGGVFLKDTDVFQRDISRLLNSNYKD
                     VFYLIISLAAVFPAFYHDIGATGNIRAFTERVDTNHQMNDLIHFVRKQVHVESSSRTV
                     ALLQRVMDFWLTGDKELLKGMVPLEVYNNLDRTFRLINLDNESIARRIYMETRHYFPE
                     YVNEKFWDFFYKVGKKRFMDAIAHHTFDGMDENEKKDALDCIVEYFDKQFPAEMTKML
                     HHIAGMFDIDTSRKQIWRFLYEIPDDDFRKL"
     gene            complement(8085..10694)
                     /gene="clpB"
                     /locus_tag="PLX22_06340"
     CDS             complement(8085..10694)
                     /gene="clpB"
                     /locus_tag="PLX22_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOT19553.1"
                     /translation="MRMDKLTLKTQNALYEAQNVATQYNHPEITPEHVLYALVNQDDG
                     IFTPIAQRLGASVDEIKRETEMALSAMPKQYGGPGGGVMSSVLRDVLNTAWNEAVTLK
                     DEYLSTEHLILGIATTGKTKARDILNAHGFTKENILRVLADIRGNKRVTDESAEDKYN
                     ALERFGRDLTKLARQNKLDPVVGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPESLKNKRIVALDMGALIAGAKYRGEFEERLKAVIEEVEQSEGDVIL
                     FIDELHVLVGAGAAEGSVDASNMLKPALARGELRCIGATTLNEYQKYIEKDKALERRF
                     QPVFTSEPSVEDTIAILRGLKERYEVHHGVRISDSAVVAAAVLSNRYITDRFLPDKAV
                     DLIDEAASLIKMEIDSMPIELDEIERKIRQLTIEREAVKKEKDKASLERLAKIEEDLA
                     NLNEKKKELSVQWQNEKKIIVAIRKIKEEIENLRIQEQKAEREGNLNLVAEIRYGKII
                     ELEKKLKALNDKLNDIQKDRKLLKEEVTEQEIAAVVSRWTGIPVSKMLEGEKEKLLKM
                     EEIIEKRVVGQEQAIQTIADAVRRSRSGLQDPNRPIGSFIFLGPTGVGKTETAKAVAE
                     FLFNDEKAIVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAIRRRPYSVVLFDE
                     IEKAHPDVFNVFLQILDEGRLTDAKGRTVDFKNTLIIMTSNIGTAYISDPAIPYEERV
                     KGVQQELKMHFKPEFLNRIDDVIVFNPLTREHINKIIMIQLNEVGKRLKERGIKLELT
                     QKALDYLVNVGFDPQFGARPLKRALQRYLLNPLSVKILGGEIMDDDTVIVDYKNNEIQ
                     FNVKRSKAEKQAV"
     gene            complement(10863..>11963)
                     /locus_tag="PLX22_06345"
     CDS             complement(10863..>11963)
                     /locus_tag="PLX22_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT19554.1"
                     /translation="DIAFVVDNSYSASREWKSIKQGIVALCDTISDNWNDMRVYVVPF
                     LTQSKKIGTYAVTGATTLDDVLQELSLAKGIVTGINPCLQYIAKGLPWRNDAKKLCIV
                     VAASRCDYSNARSLRYLIKKKGITLYVIGTGGLTHTERTALSFIGDSYYDVTYRQRMY
                     DVYGNPVDVFCEAGRIFHGDAGDRWKDGVTATKTKAAKPFVAEVFGVQNALPYELPAL
                     YPKQTAIKILNAEELENNIVDICQTIAHNNKLTGQVIARVLIADGGYSLWLPVADKRI
                     LNYLAENQHMRMYVGISPKQDLGAPYGVGLLPVAVVGLPGSHIPSMLKLTVKDIIQRP
                     QWKGFFNPPLWFIPVTVKQIIPLSGEEDIRNK"
BASE COUNT         3480 a         2654 c         2018 g         3611 t
ORIGIN      
        1 ggattatacg ttaaccgtgc aatgacctta tacttttcaa tattctctgc tgtggtttct
       61 cctttccaga tattgggatc ggttaaacat ttattccttt cagggctgcc ctgtttgtaa
      121 tacaagcaat ttctataatc accatttctg ccataatggg ggtcatcagg cggtatggta
      181 acacatatat gcggaaaatt taaaattttc tcttttggta aaaagctgtt aaccgttaca
      241 acaaaattgc cttcaccatc ataatctttc tcaggctgtg tagtatataa aacaaacatt
      301 tttttgccag taacataatt cttcataaca tataaggtgc gctctgcttt taccgtttca
      361 tcatcaatac tctgaacaac aaataccggc accgaaattg ttttaccttc ctcaaaatag
      421 ctatcaacct tttttatgag cttgtatgtt tgtgctgctc catttaaagt caacgactga
      481 tacttggtat agtcaacatc atcatcgatg tcagtccaat ctttaaatgt gcttacaata
      541 ggtgctaacc atgctacgct tgaattgata gcaaaagcgg gggcaaataa aaataagcct
      601 ttaacatcat gatttgtgtt agacaacaca tagttaaccg acaacgttgc cccggtagaa
      661 aatccaccca tataaacctc ttgtgcattt ttctttaact gaagcatgcc ataatgggta
      721 gcttttatcc actcatcata ggttaccttt aataaatcac ccggaacagt cccatgcccg
      781 ggtaaaagta gtccataaac caaaaaacct ttgcttgcaa aatacttacc tatagcctgt
      841 aaaaaatagg gggaatccga taaaccatga attaacaata ttccctttga atatatatta
      901 tttactggcc tttctgccgg tttatatata aacggactat tcatagccac aattgcttct
      961 ttattgtcag tttcaagacg agcagcagct ataaccttgg tcatcaactc aacgtactgt
     1021 tcatatggtg tttcaggtgg tagtgtaaaa gcattgttaa aaccagattc ctttagccta
     1081 ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgcaa ctctctttgg
     1201 atttaacgag atgctgatgc tatgctgcgc aattgcacag acaaaaaata ctaacgcaaa
     1261 tactatgaca aactttttca tggctcctcc tgatagtata tagtttcatt caaaaaaaca
     1321 atccttattg taccgatagt ttttcaaaat gcaacctttt ttataaaaaa agcaaactat
     1381 gcagtaacag tttttttgcc gaatatacga ttatgttttt atgaagagga aagataaaag
     1441 cactgttcat tgcactgacg atacattgca ctattgagtg gcgcaataca aaatgcaatg
     1501 gtatcttcaa ataaaaaaca taccacgaca atagcacaca tcaacctatc attgacggag
     1561 tacccacatc gattcaatgg aactaaaaaa tggcaggata atagtaaaaa atcttgtaaa
     1621 atagatgaga tttaaattta aaaaatgtat caatcaattt tataataaat taaactcttt
     1681 caagatggca tgcacttttt tatcaacact tgccaattta cgcaacgctg ccaatgcatg
     1741 ttcaagtttt ttttcgttaa tagtaacttt ttcttcaata ggctttaacg acgtttttct
     1801 atcccagaat gcaaatccta tggttgcgga ggttattgca ataaagatag ttgtcaatat
     1861 ccataaaaaa gagataacct gttcaaatcg tttatctatt tgttcaaagc gtttatctat
     1921 ttgttcaaag cgtttatcta tttcatctaa ttttgcttcc agacggataa tcctgtccct
     1981 atcctcaagg gtaaaaggga cctctttgct aaaagctgta gtagcattat aaaaaatcaa
     2041 aactatcaaa aatccaaacc ctaccctctt cacaaattat ctcccctttt ttatataact
     2101 atactaaaat aaactatcat tgtcaacagt ttattttttg acaggtattg gtattgtttc
     2161 agagacaatc cctgacatat aaggattgcc cctgaaacta tagctattgt tcaaaacaaa
     2221 gtacgcatta ccgccgtccg gtaacaagat catataagcg tgaaagtgaa gatacgtaac
     2281 caaactcatt atcataccag aagtttaagt caaccatggt atagtaatta tcgccgcgtt
     2341 tggtggttcg gtgatgggta aattctgcat catagatagt tgaataggta cttcccacga
     2401 tatccgagct tacaagctgt ttctctgagt aacacatgat atctggattt ttttctgagt
     2461 actgtttaaa tattttatgg atatactggt tatcataatc accaagaagc gaaacatcca
     2521 gaataactat cgaaccagta ttaacaggca cccttatgga tgaagcctgc gaacctatac
     2581 ccagtttctg cacttcgggt ataacctcca gaactgcctt tgcagcaccg gtgcttgtgg
     2641 gtatcatgtt gttcaaaatg ctgcgccgtt tacgggtgtc tttatcgcca tctttaggta
     2701 atacatcaag cgttgactgc gaattggtta cggcatgcac cgttgttaat gcatatgaaa
     2761 taaatttatc gccaaaatga tcaaccagcg ctttgatagg tggtgcgcaa caattggtag
     2821 tgcatgaagc attggaaatg ataacatgtt ttccagcatc atacttatca aaattaacac
     2881 cgccaactac ggtaaccgca tcatccggaa tctgtttacc tttatctttt actttaaatg
     2941 gtgctgtaac aatcaccttc ttagcatgat taagatgacc ccgcagagaa ttttcatcat
     3001 ttcgcgttgg atcaagcatc ttacccgtag tatcaaaaac aacattcacg ccaaatgtat
     3061 cccatggtat ctttccgggc accctgtagt caggctcgtt taaccagatt agctttacac
     3121 cattgaggta aacatgacca tcttttatct caaccgcgcc cttgccactg aaaccgccaa
     3181 taaacctatt aaaagaacca taggtagaat catattcaaa gtacgtcgct aaatcctcta
     3241 atgaggtacc cgtttttctt ccggttgcta taactatttc tttttgctgc ttctttgcgg
     3301 catagagcca taccagaagc ttgcctatgc gtccaattcc cataacacca attggtgcat
     3361 caggtaatac tgccataatc atcctccata tatagtaaaa taattatata cccgacagga
     3421 tgttgccgtc cccagcttcc atttttatat ttaaatttag gtaaataata ataacgctac
     3481 tattaagaat caacttccag tgactattgg aaaagctatt ataaatattt caagcttttt
     3541 tgcacataac gatattatcg atgtaccttc cgtgtgggat gcgcacccag ataaacatgc
     3601 ccggtgttgc catcaatggt tatgacatca ccctctcgaa tgactatctt ccccccctgt
     3661 tctctgttta taacagctat gttctcactt tcagcaatct tcattatgga aaaatcacta
     3721 acgcccgatt tttccagacg gcgcatctgc agtacagcat gtgatgtcat gcctcccacc
     3781 gaggttaaaa tcccatccga tttttgtaaa ccaatgacat cctcaggatt tgtttccgga
     3841 cgtatcaaaa taatcttatc tttgggatat tgcatccgta tagcttctat gcgattgata
     3901 tcaaatacag ccctgccact tacagcacct cctgaagcag ccaatccctg tcccagcagg
     3961 tactgcgata gctgctgtgg agtttctaca agctcttcta tatcaaacat atgttttgcc
     4021 ataccccgta tctgcaatat atacaataca tcattttcaa atgtaaactc aacttcgata
     4081 tcattgcccc accgcttgcg taaccggcta acagcatcgc gtagcgtagc ataatggttg
     4141 ggatatgctt tttccagcga aggatacatc gcaaagcggg caaattttaa tttctgctgt
     4201 tcgtttattg gaaatacctt tgcaacaccg ctgacaatat catgcccctg tgcccttgtt
     4261 ttatattcgc ccactatctg caactcttca tattcaatat attgactgtg cacaacaccg
     4321 gtaatggagg ttggcgatag attgccaaaa accatacgct gtgcaataac agcagtgccc
     4381 caatcatctg aaatattaat aaattggcga tagttccttg caatagggga atcccatgat
     4441 ttgaaaacag caataatcgc atagaatagc tgttcataag gatctttggg agcagagtgc
     4501 ccggctttat tgattgcata gcgatactta agggttagca ttgacatctg tttgccattg
     4561 agcttttctt tcaaatcaac accagcctca tcctttgcct gcgtcataag attttcaaag
     4621 atatgacggt ccatcccata tgctgatatt gcaaaatcgt gtatcaatcg acggtaacaa
     4681 tcccacgcaa accattcatc aaactgtgta aaatattgta caatttcctc ggtcatgcca
     4741 acattggtaa tagtatccat tacgcccggc atggaaaaaa cagcaccgct tcgcacagac
     4801 aacagtatgg gattttgggg attggcaaat ctaaatccag taaatgcatc aatatatttt
     4861 tttaacatat agatgagctt tcgtttaaac ctcggattgt ttatattgcc atcttttatc
     4921 cttttataca actctgaaga tataacaatc ccttcgggga cattaatccc atccatattt
     4981 gcagcaataa tcagcccctg tgccttggca cccacctccc acaaaggaac atagagctct
     5041 gtttcatcat aagtaccttc atattttcca atcacatgaa taagctttcc cttcttcata
     5101 cgggcatcaa ttgcaccaag tacaacaata tttcccatat aggaatacac tgaattgaga
     5161 tggtccctta attttaataa aaggttatcc attaactgca ataatggtga ctgcataacc
     5221 ctgtgacgca aaaacctgtc aacaatcacc tcgacattag caggttgatc gggaggtatg
     5281 aaatcaggga ttatattatc cattcctata tttttaatag caatacggct gactgactta
     5341 aaggtatcat taaacctgtc agagatctca ccatgaataa caagaaggct atttactatg
     5401 tcacgaaact gcgttacggt aatattaggt gcatcaagca agctggtaac cgaacgcatc
     5461 tcgtgattgg caagtccatc aatttcaaac gattggataa aaagttctac cactcgcttg
     5521 attgcatcat agtcaatttt ttgatgagga tagatgctta tattttcaat aatcttttca
     5581 aaaaggagta cccgcacaat attgcagtgg aaaaatacct ttaatgtatc aaactttttt
     5641 tctttatatg atccatacat tgatggtatg ccaaaagcaa tatgtcgttt aaactcaatt
     5701 gtatccacag gttcaaacac atcctgagac aacaggatnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnag ttttctgaaa tcatcatccg gaatttcata gagaaagcgc
     5881 catatctgtt tgcgcgatgt atcaatatca aacattccgg caatatggtg caacatcttg
     5941 gtcatttcag caggaaattg tttatcaaaa tattctacta tacaatcaag agcatccttt
     6001 ttttcatttt catccatccc atcaaaggta tggtgagcaa tagcatccat aaaacgtttt
     6061 ttgccaacct tgtagaaaaa atcccagaat ttttcgttta catattcggg aaaataatgg
     6121 cgtgtttcca tgtaaatacg ccgtgcaatt gattcattat caaggttgat aagcctgaat
     6181 gttctgtcta aattgttata tacttctaac ggcaccatac ctttgagtaa ttccttgtca
     6241 ccggtcaacc agaagtccat gactcgctgg agtaatgcaa ccgtccggct gcttgactca
     6301 acatgcacct gcttgcgcac aaaatggatg agatcgttca tctggtgatt ggtatcaaca
     6361 cgttcggtaa aggcacgtat attgccggta gcgccgatgt catgataaaa tgccgggaag
     6421 actgcagcca gtgatatgat aaggtaaaaa acatctttat agttgctgtt cagcaaccgt
     6481 gatatatcac gctggaatac atcagtgtct tttaaaaaaa caccccccag tgtaaggttg
     6541 gcaataaggc ttgcagcaag gcgtttcatg tacacaggat taagttcaat aagttcaagc
     6601 catgtgcgga tattggcaag atgactggca ttcaccataa ccgaccagtc tgaagcaata
     6661 cctgtaaatt gtggaaagca aaatcgcgca tgaacaagta tatcaacaaa aaggtcaatg
     6721 atggggcggt tattgatttc cacaacagtt ttaccgatag tatagattgt tgcaaatgca
     6781 gcattgtaat taccacctcg ttcaatctcg gatataagca ccggcaccac catctgtaca
     6841 acctgtttta gcaaatcaaa acgctgctgc tgtgcaagca caccacacag tgaagctaca
     6901 ctgcgcgata tataaagctg gagattggta tcgttaccct gctgcgattt ttttaccata
     6961 aacgatagta aagaaagcag cacgctgtca tcctgaatta tattgcgcac aatgcattca
     7021 cgggctgcaa tgcatatttt ttcccatgca gtttcattat gatgaaaatc aataacattg
     7081 attaattgct gtaacagttt ttcataatcc gtgatggtag ttcggctatc cagtgctatc
     7141 tgtaattttt tttcatacgc ttcttttgtg actgtttgaa catactggcg tagtgttgct
     7201 attgattcac catcactatt ccacaggttt cgcaatgagt ccatatattc ttcttcaacc
     7261 acaatacttc gttctacata cagtttaaat tgattgatga tgatatctgt gcacagttca
     7321 aatagctgtg catccagttt ctttcgctga agcgatagct ccatgagttg tataatacgt
     7381 tgattaacat cctccagata aaatccttcc tgagtatccg aaagtaaaat cctgcaaaaa
     7441 tcaattgctt ctttatcaaa gatggcagta acattgtcca gggaactatc gcccgcaatt
     7501 tcttttttta gtaaaaacag atagcgatcc agaaatgtat cagcagctct gattgttgaa
     7561 ccacgcttgc gtaccgttgc ttctttaaga taatggaata taacgggcag caccctatca
     7621 ctgtgaggga caatgtcata catgtaatca tacagataag ctctgatctc ggtaaccatg
     7681 tatttccagt caatgttggg atgagtcatc tcggcaacca ggtttttagc acggctcagt
     7741 gcaccaattt tatgctcaac cggcaacagt atgttattca catcgtagat ttcatattcc
     7801 atagcaacag cctctctgca tgtaataatt ataaattact ggtacagccc tatgcctgtc
     7861 aatactatta cataaacgat aattgaatag tattttaaat gttacgattc caatctcatg
     7921 atagatatcc atagtattat aattttttgg gtgaggggag agattcttcg aaactgtgtt
     7981 attctgaatg accatgaatg gcatgctatt ctgaactatg attcaggatc taaagttaaa
     8041 caaaaataag gggatgcatg gacatcccct tattgcttac atgcttacac tgcttgcttc
     8101 tcagctttac tgcgttttac attaaattgg atttcattat tcttgtaatc cacaataacg
     8161 gtatcatcat ccattatctc gccgcctaaa atttttacgg aaagtggatt gagcagatat
     8221 ctctgtaaag ccctcttcaa cggacgtgca ccaaactgtg gatcaaaccc aacgttaaca
     8281 aggtaatcta acgccttttg tgtaagctca agttttatac cgcgttcttt taaacgtttg
     8341 cccacttcat tgagctgtat catgataatt ttattgatat gctctcgggt caacgggtta
     8401 aagacaatca catcatcaat gcggtttaaa aactcaggct taaaatgcat cttcagctcc
     8461 tgctgcaccc cttttacccg ttcctcatag ggtatggcag ggtcagaaat atatgctgtc
     8521 ccaatgttgg atgtcatgat tataagggta ttcttaaagt ctactgtgcg cccttttgca
     8581 tcggtaagcc tgccttcatc cagtatttgt aaaaatacat taaacacatc cggatgtgcc
     8641 ttttcaatct catcaaacag gacaaccgaa tagggacgcc tgcggatggc ttcggtaagc
     8701 tgtccgccct cttcgtaccc aacatagcca ggtggtgcac caataaggcg tgctaccgaa
     8761 tgtttttcca tatactcgga catatcaata cgcactatcg ccttttcatc attgaacaaa
     8821 aactctgcca cagcttttgc cgtttcggtt tttcctacac ctgttggtcc taaaaatatg
     8881 aacgaaccaa tgggtctgtt gggatcctgc aatccagaac gagaacggcg tactgcatca
     8941 gcaatagttt gtattgcctg ctcctgacca accaccctct tttcaataat ctcctccatt
     9001 tttaaaagct tttctttttc accttcaagc attttactta cgggtatgcc ggtccatctg
     9061 gaaacaacag cagctatctc ctgctcggta acctcttctt ttaaaagctt tcgatctttc
     9121 tgaatatcat tcaacttatc attgagcgct tttaacttct tttcaagctc aataatctta
     9181 ccatatcgta tctcagcaac taaattcaga ttgccttcac gctcggcttt ttgctcctgt
     9241 attcttaaat tttcaatctc ttctttaatc tttcgtattg ctactatgat cttcttttca
     9301 ttctgccatt gtaccgaaag ttctttcttc ttttcattca aatttgctaa atcttcttca
     9361 atctttgcaa ggcgctctaa tgacgctttg tccttttcct tttttactgc ttcacgctca
     9421 atggtaagct ggcgtatctt acgttcaatc tcatcaagct ctattggcat ggaatcaatc
     9481 tccatcttta tgagcgaagc agcttcatca attaaatcaa ctgccttatc aggcaaaaat
     9541 ctatcagtga tatagcggtt agacagcacc gctgcagcaa ccaccgctga gtccgaaata
     9601 cgcacgccat gatgtacctc atagcgttct tttaaaccac gcaaaatggc gatagtatcc
     9661 tcaactgagg gctcactggt aaatacaggc tggaagcgtc gttccagcgc tttgtctttt
     9721 tcaatatact tttgatattc attcagggtt gttgcaccaa tgcagcgcag ctctccccgt
     9781 gccaatgcag gtttaagcat attcgatgca tcaaccgaac cttctgcagc gcctgcacca
     9841 accagcacat gcaactcatc aataaacaaa ataacatcac cttcggattg ttcaacctcc
     9901 tcaataacag ccttcaagcg ttcctcaaac tcgccacggt atttagcacc ggcaatcaat
     9961 gcgcccatat caagcgcaac aatgcgttta ttcttcaagc tttcgggaac atcaccagca
    10021 acaatacgct gtgccaaacc ttcaacaatg gcagttttac caacacccgg ctcaccaata
    10081 aggacaggat tgttttttgt tctgcgtgac agtacctgca taacacgacg aatctcctca
    10141 tctctgccaa caacaggatc aagtttgttc tgccgtgcca atttggttaa atccctgcca
    10201 aaccgttcca gcgcattata tttatcctca gccgactcat cggtaacacg tttattgcct
    10261 ctgatatcgg caagcacacg caggatattc tctttggtaa aaccatgtgc attcaatatg
    10321 tcacgggctt tagtttttcc tgttgtggca atccccagta taagatgctc ggtgcttaaa
    10381 tattcatcct ttaatgtaac tgcttcattc catgcagtat tcagcacatc acgtaaaact
    10441 gatgacataa caccgccgcc aggaccgccg tactgctttg gcattgccga aagtgccatc
    10501 tcggtttcac gcttaatttc atctaccgaa gcacccagcc gttgagctat aggcgtaaat
    10561 atgccatcat cctgattgac aagtgcatac aatacatgct cgggggttat ttcagggtgg
    10621 ttatattgtg ttgccacatt ctgcgcttca tacagtgcat tctgtgtctt taatgtcagt
    10681 ttatccatac gcataggtaa atctacctcc attaatataa aataataaaa ttacaatacc
    10741 attaatacat gaatacaata accataaaat aataaaacag gagagtgcta atgcgagttc
    10801 tataacagac aggaaattat attttatatt aatatactca atttatttcc aatcgtcaag
    10861 tattatttat ttcggatatc ttcttctccc gacaatggta taatctgctt taccgtaaca
    10921 ggaataaacc ataggggagg attaaaaaat cctttccatt gcgggcgctg aataatatcc
    10981 ttcaccgtta attttagcat tgatggaata tgactacccg gcagcccaac tactgcaacc
    11041 ggtaaaagtc ccaccccata gggtgcacct aaatcctgtt ttggagaaat gccaacatac
    11101 atgcgcatgt gttgattctc tgctaaatag ttgaggattc ttttatccgc aaccggcagc
    11161 catagcgagt atccgccatc agcaataaga acacgggcaa ttacctgacc agtcagttta
    11221 ttgttatgtg caatagtctg gcatatatca acgatattat tttccaactc ttctgcattg
    11281 agtattttta ttgctgtttg ctttggatac agcgcaggca gttcatacgg cagagcattc
    11341 tgtacaccaa acacctcggc aacaaaaggt tttgctgcct tcgtctttgt agcggtcaca
    11401 ccatccttcc atcggtcacc tgcatcacca tggaatatcc ttccagcttc gcaaaacaca
    11461 tcaactggat ttccataaac atcatacatc ctctgcctgt atgtcacatc atagtaacta
    11521 tcgccaataa acgataacgc tgtgcgctca gtatgggtta aacctcctgt gccaatcaca
    11581 tacagcgtta tgcccttttt cttgataagg taacgaagtg aacgtgcatt gctgtaatcg
    11641 caccttgaag ctgcaacaac aatacagagc ttttttgcat cattgcgcca tggcagcccc
    11701 tttgcgatat actgcaaaca cgggttaatg cctgtcacaa ttccttttgc aagcgatagc
    11761 tcctgtaaaa catcatccag agtagtagcg cctgtaacag cataggttcc aatctttttc
    11821 gattgcgtca aaaacggcac cacatatacg cgcatgtcat tccagttatc gctgattgta
    11881 tcgcacagag cgacaatacc ctgcttaatg cttttccact cgcgggaagc gctgtacgaa
    11941 ttatcaacaa caaacgcaat atc
//
LOCUS       DAOSOX010000066        11813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5388_length_11813_cov_7.237030, whole genome shotgun sequence.
ACCESSION   DAOSOX010000066 DAOSOX010000000
VERSION     DAOSOX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5388_length_11813_cov_7.237030"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1303)
                     /locus_tag="PLX22_06350"
     CDS             complement(122..1303)
                     /locus_tag="PLX22_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOT19555.1"
                     /translation="MTFFEKTDVDITMMNKGLPKINIRKDESGEIRVSFSSYQSILVK
                     KIKNIKGGRWHPDEKYWSFPNTNGILEKIFKVFESEEINLDPALQSQHPSPIIVRDKI
                     SPAEADKQSLYNFEDLRRELVSRKYSYKTIKAYIYFNKDFLNFLGKRLPDINDDDIKN
                     YLLHLAENKQSATSTLNQAINALKFYYGEVLKRKFVYEIKRPRKDKKLPVILSQEEIT
                     KILSSVDNIKHKAILMLIYSAGLRVGEVVKLKPEDIDSERMLIHIKGSKGRKDRYTIL
                     SETALEILREYWREYKPRKWLFEGARPGRYLSIRTVEKILEHACEKANIRKDVSVHTL
                     RHSFATHLLEGGTDLRYIQELLGHKDSKTTEIYTHVSIKSIGKIKSPLDTLGLKEGNN
                     K"
     gene            complement(1430..1585)
                     /locus_tag="PLX22_06355"
     CDS             complement(1430..1585)
                     /locus_tag="PLX22_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19556.1"
                     /translation="MKKRWLDAAILQSEERMHRLSGFMSIKPLIKSSEKVLSEEKIKI
                     DLTNLIP"
     gene            complement(1639..1926)
                     /locus_tag="PLX22_06360"
     CDS             complement(1639..1926)
                     /locus_tag="PLX22_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19557.1"
                     /translation="MFRENRTHEMPELFSEKNMMPDTLKKRYEKSWAKGFYEDVFCRI
                     DETLFSVLYSDKVSRPNLPVNIYVSLEILKELFGLSDEKFLGRFYFEHLNA"
     gene            2162..2377
                     /locus_tag="PLX22_06365"
     CDS             2162..2377
                     /locus_tag="PLX22_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19558.1"
                     /translation="MADFIDFVAKEAGNREMGEKFIKVLKQGDAQELKKFFDSNDYTG
                     VSIDDCKTLINNRQTIINSVDTNLKDY"
     gene            2385..5678
                     /locus_tag="PLX22_06370"
     CDS             2385..5678
                     /locus_tag="PLX22_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT19559.1"
                     /translation="MNFIKLIIFIISSLLLFSTVALSLDGNNNYVFSLSEEIMSLPKY
                     WYFRAEDREQYKEKNYDHSSWDIHAINQPWHTQKEFRNYTGNIWYRIVFNCDLCNSNN
                     YKGDFALLVPIHYRGAQFYLNGVLVKETRPFIKGISIPNLGKPDIIIFPHNLVTKENN
                     VIAIRISSFDYNSGFEGMIKIGTFPKISKVFVQNNLFYGLLIGINLFLSLYFILLFYY
                     RNKERFYLYFAGMAFSFALWTTGYIGIIFWIIDNRIAYLILAYCGSVSIVIFAILFIH
                     SFLSLRHNIFSKLLIAALVFFLIYCTIEIILTGLVVTYRMYLFRIFLLTCLIYMLYTT
                     ILCLYAIKIKRPHALKILAGIIVAYLFIFASVLTWLHIIDFTPSSIEPFMAMIFVFAF
                     VLASRYAQVFSELEKAHSKLLVLDKMKDDFLATTSHELKTPLHGIMGIVETIMDGSSG
                     PVTEKQIQNLALIRDEASHLNKMVIDILDFSKLRAGKVDLFLEHILVDQVAATIVSLL
                     TPEANKKGLSLTLKSSGPCAIVADRNRLRQIIINLVGNAIKFSDKGTITVNVESSKED
                     VTITVSDEGPGIDDSDRERIWNPFIQAESPDNRHYGGTGLGLPITKYLVELHDGSIHV
                     ESEKGKGTYFIVRLPKQPKTRGITKTKQIEVKNDSLVLPYEISPSSVEQDEELAKIPF
                     YKKAKILVVDDDEVNRKVIEGFCAIAGYELILAQDGPTALELMEKHNIDLVLLDLMLP
                     EMSGYEVCQKLRQSEKFVNIPIIIVTARDSASDMVRGFVTGANDYITKPFTRSELLIR
                     IENQLAIKQMLELERSIMHELNKEVEFNRGLYQRSIELKQMATQILAWERVIKEDMEV
                     AKVFQKRLMACPAHIPGLNFHFLYHPLAGLSGDVYDIIQLTPDIIRIFIADATGHGIT
                     ASLNTIKILSEYSSVKETMHASQDLLKFLNQRFIQLFKDYGIVFTCLIADIHLNEGKL
                     SLVSAGHPPACVISDGKVNFIKPHGPIIGLSSNYQYQPTTIPFKKKDTVLLYTDGLPE
                     FIYAQNKNFQDSSKNEYDKLKEIIEGHNTIESLENLCTSLMEGVSNRSNTFINDDITI
                     IAFQKDLGL"
     gene            5886..6485
                     /locus_tag="PLX22_06375"
     CDS             5886..6485
                     /locus_tag="PLX22_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT19560.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKNHLQEDYFKACFVEILNRAK
                     QKYHFKLIAFCVMDNHIHLVIHTLEDGAPISRIMQYIKARFAELYNKITGRTGPFWNE
                     RYKDSIIQFAKDGFHYLLWLLWYLAYNPIRKRLCSDPIKYHYSSIKAYLQEEADVGVP
                     IDHHEYFVNLGETFTERVARFMQYEEYYRKKYSMILEWV"
     gene            complement(6516..9059)
                     /locus_tag="PLX22_06380"
     CDS             complement(6516..9059)
                     /locus_tag="PLX22_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HOT19561.1"
                     /translation="MKLSFIKTIALITVSFCIVIFSTVSSFAKAKGLSVLCIYKSSEG
                     YTDDSNPLKWFFEKDITANGLQVKYYDFDKGFSSLSNLDDIRAIVTWYNGGVVASKDI
                     GINYAKFMIDAANKGIKIIIVNSYGAYGYKDGNETKWDLLPYIRPLFTKIGIYFQGFW
                     TNNPNNIKIIYKDSAMVEKDEKQDVTKSLHYQQIIPLREDVKTYLQLQRTDAPPQAGD
                     GKSSIIVISKTGAFALENYVVRGSKVMLNTSRFIHEALFYDDGYLNVGVIIGDIDRSN
                     VVNNNILYAFKYAKIRCDVYTKEEIKGLVANDLTEYEALLVATKTADAVPLKLLKGYV
                     ENGGKCIFLNYVEPDNELNTFIGIKEYIKKEEYFKQGFTLQPSLFLNGVNVPGDDIDC
                     NVRRAVLESDVKVIGTVNTLFDADKYPVVWERTIKKGKILYWNTNLLDKSKLTRGMIV
                     QSIYKIADVFATGMINVGLIMIDDFPAPWWNVAYKPYRIKYYSDLLQNEKDKFNRKKL
                     EEIIEKLKKLPEETDTLFISDVWFKDILAWQKQFGFAYSSFLIFNYNRDTKADETGFA
                     VRDFYLSQNGLSTKMGIAALENGFELGLHGYNHMSLTLTKAKEYDSVPWPDKKTMVQA
                     LTVAKNEWIALYGESALPFSYVAPHNIIDATGLQALGEVFPSIRVVSTLYVVKSGGVE
                     QEFDWSPDNRFYQIPRITSGYYFQDFDKFALYDAFQNFGIISHFIHPDDVFDEMRSKS
                     YEGWLWLKSNFEKEFGSLKQNYPWIRWMTVKDAFYEFVVYNSSTVNFKVKGNSIEIYT
                     PGGAGHNYYIRARIPKQYKQLINCNLVQYTPATGDIVVKTNKYKSVIAW"
     gene            complement(9040..11652)
                     /locus_tag="PLX22_06385"
     CDS             complement(9040..11652)
                     /locus_tag="PLX22_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19562.1"
                     /translation="MYEKQFLDLEIDNARIYKEIANFYLQNGNYNASREYYSNILKVQ
                     PLNVEANFAMGNINEFLGYWKDAKGNYEICINAQPDFEYARKAHYELQKQHAPSITGN
                     SIYFSDNIRTRYGIGLSGLYEPSQYFALQAGYELNKQTDSGGIATTGQLFYPSKGNVT
                     MHKAFAEIAVPFRFLYMRLFAHLGGTMYSGTVDYGNPLYDTSLSYMSLSYGAGFWWMP
                     PGTGVTVKVAYSHDDENDLTQSLKMKFRDAITYDMFEGILDINFSEYDFPLSKRLFWY
                     NALQYRMLSDTNTRLSSFNQLTVRVYRIPQWNMFFDIAGIYSYEDTKFNQYSSGDILL
                     VPYWAPQGVSYYGPMVKITQQVENLFGGRLLYSVFLQYQYDSLDQRIMMPGISLSQSW
                     DKLNLFAEYTYSNVTLPKSTADKTYISHDLKFGATGKFFSIYSPKGAGGKPVLFVSAN
                     PTLITPDGDGKDDFATLSLTAFDEKGIQQWWIEIYDEKEQKIQEYSLAGAVPGTFRWD
                     GTARNNVLAAEGKYYVQLTIQNSAGERFNSKKQEIVLSAAKRAIALTPSYPKFSPNDD
                     KIKDTVSFTIQATDKKNIASWFVSISNKNRIVKTIRGNDFIPYDVLWDGLDDNLKTVP
                     DGEYVAALTIKYDDGIVVKSPAATVTVVTKVAVDIKVEPNEITPLQTPLRITPSVDGD
                     VAQWQIRFMRDDGAVVKVINGAGMPPKVISWDGTDDKGEPAVYNMQLEAQMEITDDAG
                     NTAVSKKVPFMVGFLIKQEKGKRIVYMFNQDTIFKGRSDELTTHGVLILDQLAGKLAK
                     MGGFTRINIVAYTDAVGSKQSNMALSQKQAAVIAERLKNLAAEIKTVGAGAEVQYLKD
                     KPSKWDIRYEIELY"
BASE COUNT         3606 a         2341 c         1948 g         3918 t
ORIGIN      
        1 aacttagtta ttatcgaaca agttcggata taccaataaa atcggggtga tatccgaagt
       61 acttcggata ttccgaatct ggccgaacta ccagaaaact cccataggct aacaattttt
      121 gtcatttatt atttccttct ttgagtccca atgtatcaag tggactcttt atcttcccaa
      181 tacttttaat gctcacatga gtataaattt cggtcgtttt actatcttta tgtcccaata
      241 actcttgaat gtatcttaaa tcagttccgc cctccaataa atgagtggca aagctgtgtc
      301 tcaaagtatg gactgaaaca tcttttctta tatttgcctt ttcacaggca tgctccaaaa
      361 tcttttcaac ggttcttatg gataaatatc tgcctggcct tgccccttca aaaagccact
      421 tcctgggttt atattccctc caatactccc tgagaatttc taatgcagtc tccgatagaa
      481 tcgtatacct atctttccgt ccctttgacc ctttaatgtg gatcagcatt ctttcgctat
      541 ctatatcctc gggtttcagt ttgaccactt cgcccaccct taatccagca gaataaataa
      601 gcattaagat tgccttatgc ttgatattat caacagacga gaggattttt gtaatttctt
      661 cttggcttaa aataacaggc agttttttat ctttgcgcgg tcttttaatt tcataaacaa
      721 acttccgctt tagcacctca ccatagtaaa actttaatgc gttgatggct tgattcaggg
      781 tagaagttgc agattgttta ttttctgcaa gatgaagaag gtaattcttt atatcatcat
      841 cattaatgtc aggtagtctt ttacctaaga agtttagaaa atctttattg aaataaatat
      901 aggctttaat ggttttatag ctgtattttc ttgaaacaag ttctcttctc aaatcctcaa
      961 agttatagag agattgcttg tctgcttccg caggggaaat tttgtccctc acaatgatgg
     1021 gagaaggatg ctgagattgg agagcagggt ctaaattaat ttcttcactt tcaaagactt
     1081 taaaaatctt ctctaaaatt ccgttagtat tcggaaagga ccaatatttt tcatctggat
     1141 gccatctgcc tcccttaatg ttttttatct ttttcacaag aatggactga taactactaa
     1201 atgaaactct aatttcacct gactcgtctt ttctaatatt gatttttggc aatcctttat
     1261 tcatcattgt aatatcaaca tcagtctttt caaaaaaagt caatagaaat tacccataag
     1321 agctcatccc gcttttagtg gaaattccat agcagtataa cattcatatc ctcaacaaat
     1381 cttcctgtca attttaattt tttcttcgct caatactttt tctaattccc tatggtatta
     1441 aatttgtcaa gtcaatttta attttttctt cgctcaatac tttttcacta ctttttatca
     1501 gaggcttaat actcataaat ccactgagtc gatgcatccg ctcttcactc tgtaaaattg
     1561 cagcatcaag ccacctcttc ttcatgtctc ctttggccca tcttttaacc ccagtttaaa
     1621 accgttcaaa agaactattc aagcattcaa atgttcaaaa taaaacctgc ctaaaaactt
     1681 ctcgtcactc agaccaaata attccttcaa tatctcaagc gatacatata tatttactgg
     1741 cagattggga cgtgatacct tgtctgaata caatactgaa aatagtgtct cgtcaatcct
     1801 gcaaaataca tcttcgtaaa acccttttgc ccagcttttc tcatatctct tctttaaagt
     1861 atcaggcatc atattcttct cagaaaatag ttctggcatt tcatgtgttc tgttttctct
     1921 aaacacaact atttctcctt aaaaaatttt gtgcccctat actatattgt tgacatcctg
     1981 taaattaatt tatatgattt catccataaa attttattag taatactctt accctttttg
     2041 caggggggtc atatatcaat ttcattattt tcaatttatt cagacactac aaaaatatgt
     2101 tgaaatatta ttcatagtat tcattatatg tgtaattata gatatatttt tggaggtaag
     2161 tatggcagat ttcattgatt ttgttgcaaa agaagctgga aaccgtgaaa tgggggaaaa
     2221 atttattaaa gtacttaagc aaggggatgc ccaggaatta aaaaaattct ttgattccaa
     2281 tgattacacc ggcgtatcaa ttgatgactg taaaaccctt ataaacaata ggcagacaat
     2341 tatcaattca gtagacacaa atcttaagga ttattaatgc tggaatgaat tttatcaaat
     2401 tgattatatt catcatatca tcgcttttac tattcagtac tgtagcatta tctttagatg
     2461 gcaataacaa ttatgtcttt tcactttcag aagagataat gtcattacct aaatattggt
     2521 attttagggc cgaggatcgg gaacaataca aagaaaaaaa ttatgaccat tcatcatggg
     2581 atattcatgc tatcaaccaa ccctggcata cccaaaaaga attccgtaat tataccggaa
     2641 atatttggta caggattgtt tttaactgtg acctttgcaa tagcaataac tataagggtg
     2701 actttgcttt attagttcca atacactatc gtggagctca gttttattta aatggtgtat
     2761 tggttaaaga aacacgtccc ttcattaagg gaatatccat accaaacctt gggaagcccg
     2821 atattataat tttcccacat aatttagtga caaaggaaaa taatgtaatt gcaatacgga
     2881 taagctcatt tgattataat tccggtttcg aggggatgat aaaaattggg acatttccaa
     2941 aaatatcaaa agtatttgtt caaaataatt tattctacgg gcttttaatt gggattaatt
     3001 tattcctatc gttatatttt attcttcttt tttattacag aaacaaagaa cgattttatc
     3061 tttactttgc cggaatggct ttttcatttg cattatggac gaccggttat attggtatta
     3121 ttttctggat aattgataat agaatagcct atcttatatt agcatattgc ggttcggtaa
     3181 gcattgttat ctttgcaatc ttattcatac attccttttt atcgttacga cataacatat
     3241 tctcgaaatt gcttattgct gcattggtat ttttcctaat ttattgtacc atagaaataa
     3301 ttcttaccgg gctggttgta acgtaccgaa tgtatctatt caggattttt ctgctgacat
     3361 gtcttattta tatgctctat acaacaatat tatgcttgta tgcgattaag attaaaaggc
     3421 ctcatgctct taaaatattg gcagggatta ttgtagcata tttattcatc tttgcatctg
     3481 ttttaacatg gcttcatata attgatttta ctccttcatc tatagaacca tttatggcaa
     3541 tgatttttgt ttttgccttc gtgcttgctt cacggtatgc acaggtattt tccgaacttg
     3601 aaaaagcgca ttcaaaactt cttgtccttg ataaaatgaa agatgacttt cttgctacca
     3661 ccagccacga actgaaaact ccgcttcatg gaataatggg aattgttgaa acaattatgg
     3721 atggttcatc cgggccagtg accgaaaaac aaatacaaaa tctggctctg atacgggatg
     3781 aggcatcaca cctcaacaaa atggtaattg acatccttga cttcagtaag cttcgtgcag
     3841 gaaaggtgga tctattcctt gagcatatct tggttgatca ggttgcagca acaattgtat
     3901 cacttttaac tcctgaagct aataaaaagg gactatcgct tacattaaaa tcttctggac
     3961 cctgtgcgat agttgctgat cgtaaccgcc ttagacagat aataattaat cttgtgggaa
     4021 atgcaattaa attttcagat aaaggcacta ttaccgtaaa tgtagaatca tctaaagagg
     4081 atgtaacaat tacggtgagt gatgaaggcc cgggtatcga cgatagcgat agagaaagga
     4141 tttggaatcc atttatacag gcagaaagcc cggacaaccg tcattacgga ggtacaggac
     4201 ttggacttcc aataacaaaa tatttagtgg aacttcatga tgggtccatt catgttgaaa
     4261 gcgaaaaagg taaagggaca tattttattg ttagactccc aaaacagcct aaaacccgtg
     4321 gtataacgaa aaccaagcaa attgaagtaa aaaatgatag ccttgtgctt ccttatgaaa
     4381 tttctccttc atctgttgaa caggatgaag aacttgcaaa aattccattt tataaaaaag
     4441 ctaaaatcct ggttgttgat gacgatgagg ttaaccgcaa agtaattgag ggtttctgtg
     4501 caatagcagg atatgaacta atactggccc aggatggacc aacagctctt gaacttatgg
     4561 aaaaacacaa cattgatctt gtacttctag atctaatgct tccagaaatg agcgggtatg
     4621 aggtatgtca gaaattacgc cagtcagaaa aatttgtaaa catccccatt attatagtta
     4681 ctgcgcgtga cagtgcatca gatatggtta ggggttttgt taccggtgcc aacgattata
     4741 tcacaaaacc atttacccgt agtgagttac tcatcaggat tgagaatcaa ttagcaatta
     4801 aacagatgct ggagcttgag cgttcaatta tgcatgaatt aaataaagag gtcgaattta
     4861 acagaggtct ttatcaaaga agtattgagt taaagcagat ggcaacacag atacttgcat
     4921 gggagcgagt aatcaaagaa gacatggaag ttgccaaagt tttccagaaa cgtctcatgg
     4981 catgccctgc acatatacca gggctcaatt ttcatttttt gtatcatcca ttagcaggac
     5041 tcagcggtga tgtgtatgat attattcaac tcactccaga tatcatcagg atatttatag
     5101 ctgatgccac aggtcacggg attactgctt cactcaatac tataaaaatt ctttcagagt
     5161 attcctctgt taaggaaaca atgcatgcat ctcaggattt gctcaagttt ttaaaccagc
     5221 gattcattca attattcaag gattacggaa tagtttttac atgccttatt gctgatattc
     5281 atctgaatga aggcaaactt tcattagttt cagcaggtca tcctcctgca tgcgttatct
     5341 cagatggtaa agttaacttt attaaacccc atggaccaat cattgggctt tcttccaatt
     5401 atcaatatca gcctacaaca atccctttta aaaagaaaga tacagtgctt ctttatactg
     5461 atgggctgcc tgaattcatt tatgctcaaa ataaaaactt ccaagattct tcaaaaaatg
     5521 agtatgataa gctaaaagaa ataattgaag gtcataatac tatcgagtca ttagaaaatc
     5581 tgtgtacatc gcttatggaa ggagtttcta atagatctaa cacatttatc aatgacgaca
     5641 taactattat tgcttttcaa aaagatttag ggctctgacc cgattttctc cttttcctct
     5701 gcatactccg cattctctgc gtgattattt tttttaaaaa ttactatgct ctgacccctt
     5761 tttttttcaa tttttttatc aaaaagcaca aaattttttc taaatctttt taatttttta
     5821 aacttcacgc gttgtataga gtaaaagcgt aatgtaaata ttactacata cacggaggaa
     5881 tacctatggc acggaaattg cgtgtacagc taccaggttt aacgtaccac atcacttccc
     5941 gctgtattga gtggaaaaac cacctgcagg aagactactt caaagcctgt tttgtggaaa
     6001 tactgaacag agcaaaacaa aaatatcatt ttaagctcat tgccttctgt gttatggata
     6061 atcatatcca tttagttatt cacacccttg aggatggtgc gcccatttcc cgtattatgc
     6121 agtatattaa ggcgcggttt gctgaactgt ataataaaat aaccggcaga accggtcctt
     6181 tctggaatga gcggtataag gatagcatca tacagtttgc aaaggatggg tttcactatt
     6241 tgttgtggtt attatggtat ctggcatata atcccatacg aaagaggtta tgctctgacc
     6301 ctataaaata ccactacagc agcattaaag catatttaca ggaagaagct gatgtaggag
     6361 tacccataga ccatcatgag tactttgtta atcttgggga aacgtttaca gagagggtgg
     6421 caagattcat gcagtatgag gaatattaca ggaagaagta ttccatgata cttgagtggg
     6481 tgtaaaaggg gtcagagctg ttattaagtt agtaattacc acgctattac tgacttatac
     6541 ttatttgttt taacaacaat atccccagtt gctggtgtat attgcactaa gttacaatta
     6601 ataagctgct tatactgttt ggggatacgt gctcgtatat aataattgtg ccctgctccg
     6661 ccaggagtgt atatttcaat actgttaccc ttaactttaa aattaacagt agaagaatta
     6721 tatacaacaa actcatagaa agcatctttc acagtcatcc atcgtatcca ggggtagttt
     6781 tgctttaatg aaccaaattc tttttcaaag ttgcttttca gccaaagcca cccttcataa
     6841 gattttgaac gcatttcgtc aaagacatca tcaggatgga taaaatggct gattattcca
     6901 aaattttgga acgcatcgta caaagcaaat ttatcaaaat cctgaaaata atatcctgat
     6961 gtaatacgtg gtatctggta aaacctgtta tcaggtgacc agtcaaattc ttgttctact
     7021 ccgccacttt ttactacata tagcgtcgac acaactcgta tactggggaa aacctctccc
     7081 agcgcctgca atcctgttgc atcaattata ttatgcggtg caacatagct aaaagggaga
     7141 gcactttcgc catacagtgc tatccattca ttttttgcta cagtgagagc ctgaaccatt
     7201 gtttttttat ccggccacgg tactgagtca tactcttttg cctttgtaag agttaatgac
     7261 atatggttgt acccatgcag gccaagttca aacccatttt caagagctgc tattcccatt
     7321 ttagtactaa gtccattctg cgataaatag aaatctcgta ctgcaaagcc tgtttcatct
     7381 gcttttgtat cacggttata attaaaaatt aaaaaactgg aataagcaaa tccaaactgc
     7441 ttttgccatg ccagtatatc tttaaaccat acatcagaaa taaaaagggt gtctgtttct
     7501 tcaggtagtt tctttaattt ttctatgatt tcttccagct tcttgcggtt gaatttatcc
     7561 ttttcgtttt gtaacaagtc actgtaatat ttaattctgt atggcttata tgcaacattc
     7621 caccatggtg cagggaaatc gtcgatcata attaaaccaa catttatcat ccctgttgca
     7681 aacacgtctg caattttata aatgctctga acaatcatcc cgcgggtcaa cttagattta
     7741 tccagcaaat tagtattcca gtacaatatc ttacctttct taattgttcg ttcccataca
     7801 acaggatatt tatctgcatc aaataatgta ttaaccgtac caataacttt gacatcactt
     7861 tccagaactg ccctgcgaac attacagtct atatcatcac cgggtacatt tactccattt
     7921 aaaaacaatg agggttgtaa cgtaaaaccc tgtttaaaat attcttcttt ttttatatat
     7981 tctttaattc ctataaacgt attcaattca ttatctggtt caacataatt taaaaatata
     8041 cattttccac cattttcaac ataacctttc aataacttta atggaacagc atctgcagtt
     8101 ttagttgcca ccagtagcgc ttcatattca gttaaatcat tagccaccaa tccttttatt
     8161 tcttcttttg tatacacatc acagcgaatc tttgcatatt taaaggcata gagtatatta
     8221 ttgtttacta catttgatct gtctatgtca ccaattatca cacccacatt aagatagcca
     8281 tcatcataaa agagtgcttc atgtataaac cgtgatgtat tgagcattac tttgctccca
     8341 cgaaccacgt agttttctaa tgcaaatgct cccgtctttg atataacaat aatacttgat
     8401 ttgccatccc ctgcttgagg cggtgcatcg gttcgttgta gctgtaaata tgtctttaca
     8461 tcttcacgca atgggataat ctgctgatag tgcaatgatt ttgttacatc ctgcttttca
     8521 tccttttcta ccattgcact gtctttatat atgatcttta tattgtttgg attgttggtc
     8581 cagaatccct ggaagtaaat ccctatcttt gtaaaaagag ggcgtatata gggaagtaaa
     8641 tcccattttg tttcgttgcc atccttatag ccatatgcac cgtaggaatt gacaatgata
     8701 atttttatcc ctttattggc tgcatcaatc atgaactttg catagtttat acctatgtcc
     8761 ttacttgcca caacaccgcc attataccag gtaactatcg cccgtatatc atcaagatta
     8821 cttaacgatg aaaagccctt atcaaaatca taatatttta cctgcaaacc atttgcggta
     8881 atatcctttt caaaaaacca ttttaacggg ttactatcat cggtataccc ttcggatgat
     8941 ttatagatac acaataccga aagccctttt gcttttgcaa aagaactcac tgtgctaaat
     9001 ataacaatac agaaagatac tgttattaaa gcgatagttt taataaagct caatttcata
     9061 acgtatatcc cattttgaag gtttatcttt aaggtactgt acttctgcac ctgcaccaac
     9121 agtttttatt tctgcagcaa gattttttaa tctttcagct attactgcag cctgtttttg
     9181 cgataaagcc atattggatt gcttgcttcc tacagcatca gtataggcaa caatattaat
     9241 ccttgtaaag ccacccatct ttgccagttt acctgcaagc tggtcaagta tcagcacccc
     9301 atgggttgta agctcatcac ttcgtccttt aaatattgta tcctgattga acatatatac
     9361 aatacgtttg cccttctcct gctttatcag aaatcccacc ataaacggta ctttctttga
     9421 tactgcggta ttgcctgcat catctgttat ctccatctgg gcttctaatt gcatattata
     9481 cactgcaggt tcacctttat catcggtccc atcccaggaa attactttag gcggcatgcc
     9541 tgcaccatta attactttaa caacagcacc atcatcgcgc ataaatctaa tctgccactg
     9601 cgcaacatct ccatcaactg atggagtaat acgcaatggt gtttgcagcg gtgtaatttc
     9661 atttggctca acttttatgt caactgctac tttagtaacg acagtaacag tagcagctgg
     9721 agacttaacg acaatcccat catcatattt tatagtaagt gcagctacat attctccatc
     9781 aggtactgtt ttgagattat catctaatcc gtcccataat acatcatacg ggataaagtc
     9841 atttcctcta attgttttta caattctatt tttattgctt atagaaacaa accagctggc
     9901 tatgtttttc ttgtccgttg cctgaatagt aaaacttact gtatccttaa ttttatcatc
     9961 atttggggaa aatttgggat atgaaggggt cagagcaata gcacgttttg ccgcagatag
    10021 tacaatctcc tgctttttag agttgaatcg ctcacctgca gaattttgta tggtaagctg
    10081 aacatagtat ttgccttccg cagccagcac attgtttctt gccgtaccat cccatctgaa
    10141 tgttcccggc accgcaccag caaggctata ttcctgaatc ttctgctctt tttcatcata
    10201 aatttcaatc caccactgct ggataccttt ttcatcaaat gcagttaaac tcaacgttgc
    10261 aaagtcatcc ttgccatcgc catcaggtgt aataagcgtt ggattggctg acacaaatag
    10321 tactggttta ccaccggccc cttttgggct atatatgctg aagaatttac ctgttgcacc
    10381 aaacttcagg tcatgtgaga tatatgtttt atcagcagtg gatttgggca gagtaacatt
    10441 tgaatacgta tactctgcaa acagattaag tttatcccag ctctgtgaaa gtgagattcc
    10501 tggcatcatt atgcgctgat caagtgaatc atattgatac tgcaaaaata cagaatacaa
    10561 caaccgcccg ccaaaaagat tttcaacctg ctgcgttatt ttaaccattg ggccataata
    10621 agaaacaccc tgcggagccc agtatggaac aagtagtata tctccggaag aatactggtt
    10681 aaattttgta tcctcgtacg aataaatacc tgcaatatca aagaacatat tccattgcgg
    10741 tatacgatat actcgtactg taagctggtt aaagctagac aatcgagtat tggtatccga
    10801 tagcatgcgg tattgaagcg cattatacca gaaaagccgt ttactcaatg ggaaatcata
    10861 ttcagaaaaa tttatatcaa gaataccctc aaacatatca tacgtaatgg catctctaaa
    10921 cttcatcttt aacgactgtg ttagatcatt ttcatcatca tgactatacg ctacttttac
    10981 ggtaactcca gtacctggcg gcatccacca gaacccagcc ccataggaca atgacatata
    11041 acttagtgat gtatcataca aaggattgcc gtaatctacc gtacccgaat acatggtacc
    11101 gcccagatgt gcaaaaagcc gcatatacaa aaacctgaag ggaactgcaa tttcagcaaa
    11161 agctttgtgc attgtcacat tccctttaga tggataaaag agctgcccgg ttgtagcaat
    11221 gcctccagaa tcagtttgtt tattcaattc atagcccgcc tgtaatgcaa aatactggct
    11281 gggctcatat agaccactta agccaatacc atagcgtgtg cgtatattgt cactaaaata
    11341 tatactattg ccagtaatag aaggagcatg ttgtttttgt aattcataat gagccttacg
    11401 tgcatattca aaatccggct gtgcattaat gcatatttca tagtttccct tagcatcctt
    11461 ccagtatccc aaaaattcat tgatattgcc catagcaaaa tttgcttcca cgttcaacgg
    11521 ctgcactttt aaaatatttg aataatattc tcgtgaggca ttataattgc cgttttgcaa
    11581 atagaagttg gctatttctt tataaatacg cgcattgtct atctccaggt ctaagaactg
    11641 cttttcatac acattgcgca ttgtggcaag gccaaatgaa agcttttggt taacatcatc
    11701 atcaatagat tccagtaatg agtctgtatt tgccagtaaa acttcaatat cctgaagtgg
    11761 taactactca gaaagtagag gcacactcag gggaaaatat agattgtttg att
//
LOCUS       DAOSOX010000067        11575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5553_length_11575_cov_7.129688, whole genome shotgun sequence.
ACCESSION   DAOSOX010000067 DAOSOX010000000
VERSION     DAOSOX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5553_length_11575_cov_7.129688"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1472
                     /locus_tag="PLX22_06390"
     CDS             282..1472
                     /locus_tag="PLX22_06390"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19563.1"
                     /translation="MHHKQKLYTNPNLIIAFSITLIVVMGVTNITPALPAMATFFNIP
                     YSSVALLITVFTMPGIIVTPILGIVSDRIGRRILIIPSLLIFGITGTIMFFVKDFSWL
                     LILRFIQGVGVSALGAINTTIIGDMFTGNDRTAAFGYNASVLNIGTAAYPVIGGLLCI
                     AGWNYPFLLSAFAFVVAWFVIFHLHNPEPVNEVALKDYFKTIAAIIKKPYIIGLLSTN
                     ILTFTLLYGPIITYFPYIISYRFNGDSATIGLIMSLMSIVTAITATQLQWLSTRFSEK
                     NLIFYSFLGFAISFIIAGLAQNYFWLTVAIIIGGASNAINNPSLLSLLTGAAPTHYRG
                     AIMSLNGMGLRVGQTVGPIIMAMLLAIVTIDIAFYIIGGATVIITIALFSTLLSNREY
                     TVKT"
     gene            2024..2221
                     /locus_tag="PLX22_06395"
     CDS             2024..2221
                     /locus_tag="PLX22_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19564.1"
                     /translation="MKLTKEIEQGQADLSIEIEKIQTDLTREIHQSHTSTLRWMIGLF
                     LAAVLTQAGLIIALLQLAISK"
     gene            2415..3818
                     /locus_tag="PLX22_06400"
     CDS             2415..3818
                     /locus_tag="PLX22_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT19565.1"
                     /translation="MNNNERLAIIQQKLSAALGASVVSNDPDVLEKYAIDETSDLKGN
                     PWLVVYAKSAEDVSTVLSVCNEYCIPVIPRGAGTGVTGGAVPVCGGVVLSLETMNSIV
                     EIDVENMIAVCEPGVITGILQKEVAQYGLMYPPDPASLDSCSLGGNVAEGAGGPRAVK
                     YGTTKDYITGLEFVLSDGSIIQHGGKFIKNATGYNLTGILIGSEGTLAVITKIYCKLI
                     PAPAYTLDMLIPFASLEEAIRMVYTFLINRIKPAVLEFMEEDALIVASQYLGQAMPHP
                     EAKAHLLIQLDGDSSEDIERMLQTILSLGMVHPDTILTAQSPQQKERLWKARRCIREA
                     IDAKSPVFLAEDCSVPRSSIAPFLTGVKEALTPYGLQSIIFGHAGDGNVHIDVLKNDM
                     PYEDFKKLVPALKEIIYGQAYRYGGTITGEHGTGFIRKDALKKFATSQELSLYKRLKK
                     AFDPNGILNPHKIIDID"
     gene            complement(3833..4294)
                     /locus_tag="PLX22_06405"
     CDS             complement(3833..4294)
                     /locus_tag="PLX22_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT19566.1"
                     /translation="MQCEVPAFNPGDSEIKNILLNNKIIAVVGLSDDPAKDSNRVARY
                     LINHGYTVVPVNPTKQSILGLQSYPNIKAIPFPVDIVDIFRKPADVPPIVDDAIAIGA
                     KVVWMQLGIAHNEAAKKANNAGLKVVQGKCIKVEHSRYFGNSGQTVQFNIS"
     gene            complement(4474..6132)
                     /locus_tag="PLX22_06410"
     CDS             complement(4474..6132)
                     /locus_tag="PLX22_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HOT19567.1"
                     /translation="MENKTYEPGQAYEYQLTLDKLLKAALVYNPNQEIVYRDIIRYTY
                     KDLMRRIHKLANVLKGMGVKQGDTVAIFDYDSHRYLECFFAVPMMGAVLQTMNFRLSP
                     EQIVYTLNHTGATTILVHGDMLPLISSIRDKLHNVCHCIVLVDDVKPLADDIAITGEY
                     EAMIRVADDRYDFPELDENTKATTFYTTGTTGLPKGVYFSHRQLVLHTFGLMAMLGAY
                     DNIGRFRSGDVYMPLTPMFHVHAWGIPYLATLLGVKQVYPGKYEPAMLLKLIVKEKVT
                     FSHCVPTIIQMLVTAPGIESVDLSHWKVVIGGAMLPEDLCKQALDKGIDIYSAYGMSE
                     TCPLLTAALLKPTLLSIPKDQQVAFRCKTGLPAVLADVQVVDAHDNPVEQGMTGEVVV
                     RSPWLTKGYYKDEDKTKELWKNGWLHTGDVGFIDEYNYLKITDRIKDVIKTGGEWVSS
                     LDLENIMSQHPAVFECAAVGVPDPKWGERPLLVIKLKDDCKNTVDDKQLHAFMLKAAK
                     DGKIPKYGVPDKFEFVDAIPKTSVGKIDKKEIRKKYSQPVAATI"
     gene            complement(6228..6905)
                     /locus_tag="PLX22_06415"
     CDS             complement(6228..6905)
                     /locus_tag="PLX22_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT19568.1"
                     /translation="MNKKISIVIVEDHPVFRMGLCKIINQESDMMVVGEAEDVHNGLK
                     IISKLKPDAAIIDISLKDSNGIELVKDIARHFPVVKTLVLSMHDEKIYAQRVLQTGAS
                     GYIMKDKTSDVIVEAIRTILDGSIYVSEQIMKEIVAKAVKNEYGQSHVDILTNRELAI
                     FALIGKGKTTSEIAATLNLSSKTIGTYKERIKEKLHIKNAVQLVNEATRWVHETEENV
                     SQLPTNK"
     gene            complement(6913..8670)
                     /locus_tag="PLX22_06420"
     CDS             complement(6913..8670)
                     /locus_tag="PLX22_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT19569.1"
                     /translation="MPMESNSNNTPAFNDRYITIFENTGAGTILIDADTTIILVNSRF
                     ASMLGYDKQEIEGRVSWTQFIHEDDVERMKEYHCLRRKDPDSAPKNYEFRIYTKDKQI
                     LYIYITIDMIPGTQLSVASLTDITQLVETREALRKSEEKYRLLVETMSDGLLVQDEHL
                     TIQYVNPRFCDIVGYDQQYIIGKQIKDFIHPHYYCQFDIQMEKRQFGIQEPYEMAWMK
                     DDGTIAHTIVSPKIVSDEQGRFLGSFAVVTDITQRKNWEEALKQSEELFAKVFDATPV
                     GLALIDMQTGMIVKWNKAFEILIHVPLHHKPAISIFDIGIIKKRDIQRLYKQLIKFNT
                     ISDYEISLKHDSKEYIAILSAEVIYINDQKMVIISLTDITEKRMLEKEIILISEKERQ
                     TIGQDLHDDLIPHLLGIDALCKVMIQELSKNILPLKEDVENIRQLLDGAIVKTRQFSR
                     GLTPSYFIEKQGLSALVHELIQTVAMIYGITCTYICDKEISISNSEVLTHVYHIIQEA
                     VYNAVKHSKSETINIVITETHKTIMIVIEDKGYGFDIAHVRGMGLKIMRHRAAVIGAE
                     LKITSMVNAGTTISLTIDK"
     gene            complement(8729..9799)
                     /locus_tag="PLX22_06425"
     CDS             complement(8729..9799)
                     /locus_tag="PLX22_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT19570.1"
                     /translation="MKTLPLLDTWEALQQLPWLSLGKFPTPVQRLHHFSHDNLWIKRD
                     DLSSEVYGGNKVRKLEFTLAEAKALNKSRVVTMGGIGTNHGLATAMFCQKLGLACRLL
                     LFYQPVTAYVKKNLLLLCRYNAQLKYYKTMLKAGVMFYTFERLMHDAYFLYAGGSSPL
                     GTVGFVNAAFELQQQIQQGLMLEPDYIVCALGSAGTMAGLTLGAALAGLRSKVIGVRV
                     TDRSLGPVPIANEKSVLSLMKQTYALMAGFTQLPAVDFMTPAIVHDYCGEGYGYPTAA
                     CMDAIDTMHQLENIPLEPTYTGKTFAAVCGMIQNPEYNNKTILYWHTYNSVDLTEEAA
                     HADYLDLPKNLHWVFEERSNAC"
     gene            complement(9803..11167)
                     /locus_tag="PLX22_06430"
     CDS             complement(9803..11167)
                     /locus_tag="PLX22_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOT19571.1"
                     /translation="MNSNKTMNLKRVLGLAEVTFIAVGFTIGGGIFVFTGIVLKIVGP
                     ALPLAYLLAVIPVFLSMLPLAMLGSAIPSVGGNYKYPSRMVSPGVAFAGIWIYVLATF
                     FGQIPLYSISCARYIATFFPAMSVQCMAIVLVTVFYLVNLIGIKPASVLQAIMVIILA
                     SALLFFSFAGIVHLDGKNFENFFQKGIGNLAVGIALLTFTYLGSNGIIELGGEIKDAG
                     KTIPRAYFITFPIVAIVYLLVAIAATGALPWHTTSQAEEPLVFISKSILDSAGFMFFI
                     LGGAVLALITTLNALFIVGTKSLLIIINDRLLPAWLGNVHKKFATPHILLTIIWLLSL
                     AGILSNFSIATFASYSALGGIIVFLPILIAALRFPKLYPERYMQAHFKLKGVWLYLCT
                     FAGFILIVFFCIIILVDLQSLLKIGLFLLFALSGVAYYYLRKRFLLRQGFDIVAIATK
                     EDWR"
BASE COUNT         3435 a         2495 c         2054 g         3591 t
ORIGIN      
        1 aaactattga ttaatcataa gactacgtaa atacaaattg tcaatacttt tttaagggtt
       61 gtcttaattt tcaacacata tcgcgatatc aacattaata aaaaagaaat aaaaaaaaat
      121 gaatggcttt aatcaatatt tatagcaaca ccttgcttac tataagggta actatcgccc
      181 aaaaaattaa agaaaacatt gtgttttgtg cttgattggt actacactgg tatatttaca
      241 tacatagcca ttgataacca taaacacata agcggtagcc tatgcaccat aaacaaaagc
      301 tctataccaa ccccaacctg attattgcat ttagcattac gcttattgtt gtcatgggtg
      361 ttacaaacat tacgcctgca ttacctgcta tggcaacttt ttttaatatc ccttattcat
      421 cggttgccct gttaatcact gtttttacca tgcccggaat tattgtaacg cctatccttg
      481 gcatagtatc cgacaggatt ggaagaagaa ttttaataat cccctcactg cttatatttg
      541 gcataaccgg taccatcatg ttttttgtta aggatttttc atggctcctt atactgcgct
      601 ttatacaggg cgtgggagtt tcggcattgg gcgctatcaa cacaaccatc attggcgaca
      661 tgtttaccgg taacgaccgc actgcagcat ttggttataa cgcaagtgtt ttaaatattg
      721 gcaccgcagc ctatcctgtc attggcggac tgttatgtat tgctggctgg aactatccct
      781 ttttgctttc agcctttgcc tttgttgttg catggtttgt tatatttcac ctgcacaacc
      841 cagagcctgt caatgaagtt gcactgaaag actatttcaa aaccatagct gccattatca
      901 aaaaacccta tattataggg ctactgtcta ccaatatcct tacctttacc cttttatatg
      961 gacccattat tacctatttt ccctacatca tctcataccg gttcaacggc gattctgcaa
     1021 caatagggct tatcatgtca ctgatgtcca ttgtaacagc aataacagct acacagctac
     1081 aatggctttc aacgcgcttt tcagaaaaaa atcttatctt ttacagcttt cttggttttg
     1141 ccatttcatt catcattgca gggctggctc aaaactattt ctggcttacg gtggcaatca
     1201 ttataggtgg cgcttccaac gctatcaaca acccaagttt gctttcactg ttaacaggcg
     1261 ctgcacccac acactatcgc ggagccatca tgtcattaaa tgggatgggg cttcgtgttg
     1321 gtcaaacagt tggtccaatc attatggcaa tgttattggc gatagttact attgatatag
     1381 cattctacat tataggcggg gcaacggtaa tcattaccat agcactgttt tccaccctgc
     1441 tatcgaatag agaatacacc gtgaaaacat gattaactat cgatacctct cgttgattgt
     1501 acgtactacc caggatagcg gaactactca ttcctgagaa acttcaggct gttatctgct
     1561 caatctgcca tgccgttgct atcgcttgtt tattatgact attgccgttc aactgtgctt
     1621 tatcaaatac ttattcccat attggcaatc tcattcttca ccaacctcgt tgttattagt
     1681 gttatacaac cctccagaag gaaaaaaatt aatacttttt ttcaccatcg ccccctgtta
     1741 ttgaggttac aatgttgatt catgtcaatt ttttaaaaaa agagatggta gtgaagtatg
     1801 gatggtatta tacatgcatt aaaataattt gacatgttac cagcaggcag tagtatatat
     1861 acataaagga ggcaacgtac caatgggata agcagtaaga atatatgagg catttaagga
     1921 agacgagatt aaggcaaaac tattagctga gtttattgag ttagttgagc agacaatatt
     1981 aaataatcag ttagccaccc ggcaggatgt aacgatctca gaattgaagc tgaccaaaga
     2041 gatagagcaa ggacaggcag atttaagtat agagattgag aaaatacaaa cggatttaac
     2101 cagagagata catcaaagcc atacaagcac attacgctgg atgataggtt tatttctcgc
     2161 tgccgtgtta acacaggcag ggcttatcat agcgttgttg cagcttgcta taagtaaata
     2221 aggttaatgt acatatcata cagtttaaca caaaaagcct gaatatttac aataataaaa
     2281 aataaaatag aaaatcattc tacaccccct tgcacaacaa taccctgctc atagacaaaa
     2341 tgacatagat tgaatagtat tcccactcct aaaaaagctt gcatcaatac actgcatacc
     2401 ctatgtatgt catcatgaac aacaatgaaa ggttagccat tatacaacaa aagttgagcg
     2461 ctgcacttgg agcatctgtt gtcagcaatg atcccgatgt gcttgaaaaa tatgccattg
     2521 acgaaacatc cgatcttaaa ggcaatccat ggctggtagt gtatgcaaaa agtgctgagg
     2581 atgtgagcac tgttctttca gtctgtaacg aatattgtat accggtgatt ccacgcgggg
     2641 cgggtaccgg tgttaccggc ggtgctgtac ctgtttgcgg tggtgttgtt ttatcattag
     2701 aaacaatgaa tagcatcgtt gagatagatg tcgaaaatat gatagcagtg tgcgaacctg
     2761 gcgtcattac gggtatattg caaaaagagg tagcacagta tggacttatg tacccgcccg
     2821 atccggcaag ccttgattca tgctcacttg gtggcaatgt agctgaaggt gcaggaggac
     2881 ctcgcgcagt aaaatatggc acaacaaaag attatatcac agggcttgag tttgtactta
     2941 gtgatggaag tatcattcag catggtggta aatttataaa aaatgctaca gggtacaatc
     3001 ttaccggcat tctcattggt tctgaaggaa cacttgccgt tattacaaag atatactgca
     3061 agctcatccc tgctccggct tatacacttg atatgcttat cccatttgca agtcttgaag
     3121 aagcaatcag gatggtgtac acgtttttaa ttaatcgcat taagcctgct gttttagaat
     3181 ttatggaaga ggatgccctt atagtagcct cacagtattt aggtcaggca atgcctcatc
     3241 cagaagcaaa agctcatctg ctcattcagc ttgatggcga tagttccgaa gatattgagc
     3301 gcatgctgca aacgattctt tcccttggaa tggtacatcc cgatacaata ttgacagcac
     3361 aatcacccca acaaaaagaa cggctatgga aggcacggcg ttgtatccgt gaggcaatag
     3421 atgcaaaaag ccccgttttt ttagctgagg attgctcagt cccccgctct tctattgctc
     3481 cttttttaac gggggtcaaa gaggctctga ccccgtatgg cttacaatcg atcatatttg
     3541 ggcatgccgg tgatgggaac gttcatattg atgtgctaaa aaacgatatg ccctatgagg
     3601 attttaaaaa gctggtacca gcgcttaaag agattatata cgggcaggct tatcgatacg
     3661 gtggtactat cactggcgag catggaacag gatttatacg gaaagatgca ttaaagaagt
     3721 ttgctacttc ccaggaacta tcgctttata agcgtttaaa aaaagccttt gatcccaacg
     3781 gaattttaaa tccgcacaag attattgata tagattgatg ccgataacat tattaactaa
     3841 tattaaattg tactgtttgt ccactgttgc caaaataacg ggagtgctct accttaatgc
     3901 acttgccttg aactacttta agccccgcat tgttagcttt ttttgctgct tcattatgag
     3961 caatccctaa ctgcatccat acaacctttg caccaattgc aatggcatca tcaacaatgg
     4021 gcgggacatc cgcaggtttt ctaaaaatat ccacaatatc aacagggaac gggattgctt
     4081 ttatattggg gtacgactgc aaacccaata tgctttgttt cgttggatta accggcacca
     4141 ctgtgtagcc gtgattaatt aaatagcgtg caacacgatt gctatctttt gctggatcat
     4201 cagacaatcc caccactgca ataattttat tgttcaacaa aatgttttta atttcactat
     4261 cacctggatt gaatgcaggt acttcgcatt gcatatctgc cataccacct cctatccccg
     4321 gcttttattc aatatgcagt actataatct ataataaata taaacacatt ttcatttttt
     4381 tacaatttaa atatgtgaat atacctatgc ttttctgaaa acagggggtc agagcatcac
     4441 atagtgaagg catatgctct gacccttctg gcattatatg gttgctgcca ctggttggct
     4501 atattttttc ctgatctctt ttttatcaat ctttcctaca cttgttttag ggatagcatc
     4561 aacaaactca aatttatccg gaactccata ttttggaatt ttaccatctt tagcagcctt
     4621 tagcataaag gcatggagct gtttatcatc gactgtattc ttacaatcat cttttagttt
     4681 tataaccaat aatggccttt caccccactt gggatccggg acgccaaccg ctgcacattc
     4741 aaaaacggct ggatgttgac tcataatatt ttccagatca agcgacgata cccattcacc
     4801 gcctgttttg ataacatctt taattctgtc cgttattttc aaatagttgt attcatcaat
     4861 aaatcccaca tcccctgtat gcaaccatcc atttttccat aactctttag ttttgtcttc
     4921 atctttgtag tatccctttg taagccatgg ggaacgcaca acaacctccc cggtcatacc
     4981 ttgctccacc ggattatcat gagcatcaac aacctgtaca tccgctaaaa ctgcaggtaa
     5041 ccctgttttg catctaaacg caacctgctg atcctttggt attgatagta gtgttggttt
     5101 taataacgca gcagttagca atggacatgt ttcagacatc ccataagccg aatagatatc
     5161 aataccttta tccaatgcct gtttacatag atcctctgga agcattgctc caccaataac
     5221 aaccttccag tgagatagat caacactctc aatccctgga gcagtaacaa gcatctggat
     5281 aatggttgga acgcaatggg aaaaagtaac cttttctttt actattagct ttaataacat
     5341 tgcaggctca tatttccctg gataaacctg ttttacaccc agcaatgttg caaggtaggg
     5401 gattccccat gcatggacat ggaacatagg tgttaatggc atataaacat cacctgatcg
     5461 gaatctgccg atattgtcat aggcgccaag cattgccata agcccaaatg tatggaggac
     5521 cagttgccga tggctaaagt aaaccccctt tggcaaacct gttgttcctg tggtataaaa
     5581 tgttgttgct tttgtatttt catccaattc cggaaaatca tacctgtcat cagcaacccg
     5641 tatcattgct tcatattcac cggttattgc aatatcatcc gccaatggtt tcacatcatc
     5701 aacaagcact atacaatggc aaacattatg cagcttatcc ctgatactgc taatcaatgg
     5761 gagcatatca ccatgtacca gaatagttgt ggctccggta tggtttaatg tgtaaacaat
     5821 ctgttcaggt gataatctaa aattcatcgt ttgcagcacc gcccccatca taggcacagc
     5881 aaaaaaacat tccagatacc ggtgactatc ataatcaaag atagcaacag tatcaccctg
     5941 tttcactccc atgcctttca atacatttgc aagtttatga atacgacgca ttaagtcctt
     6001 ataggtatac cgaatgatat ctcgatatac aatttcctgg ttgggattat acacgagtgc
     6061 tgcctttaat aatttgtcca gcgttagctg atactcatac gcctgccctg gttcatacgt
     6121 tttgttttcc atcaataaac ctcctgctgt tttatttata gagatagtaa caacgatgat
     6181 attgtcttac aatacttgga attattttat tgatgtacca ttgatactta tttatttgta
     6241 ggtaattgac ttacattttc ttcagtttca tgcacccatc gggtagcttc attaacaagc
     6301 tgtactgcat tttttatatg taatttttct tttatgcgtt ctttatatgt tcctatagtt
     6361 ttactgctta aattaagtgt tgctgcaatc tcgctggttg tttttccttt tcctattaat
     6421 gcaaatattg ccagctctct gtttgtcagt atatcaacat gtgactgtcc atactcattc
     6481 tttacggctt tagctacaat ctctttcata atctgctcac taacgtagat agacccatcc
     6541 agaatggttc taatcgcttc cactattaca tccgatgttt tatctttcat aatatatcct
     6601 gatgcaccag tctgcaatac tctttgtgca taaatctttt catcatgcat tgataaaacc
     6661 aatgttttaa caacagggaa atgacgtgct atatccttta ccagttcaat cccattgcta
     6721 tcttttaacg atatatcaat aatagcagca tcaggtttta attttgatat aatttttaat
     6781 ccgttatgca catcctcggc ttcacccaca accatcatgt cgctttcctg attaattatt
     6841 ttgcacaagc ccatcctgaa aacgggatga tcttcaacaa ttacgataga tatttttttg
     6901 ttcatagtac ccttatttat cgatagttag cgatattgtt gttccggcat ttaccattga
     6961 agttattttt aattctgcac caataactgc cgccctgtgc ctcatgatct ttaatcccat
     7021 accacgaaca tgagcgatgt caaaaccata ccctttatct tctataacga tcataatagt
     7081 tttatgtgtt tcagtaatca caatgtttat tgtttcagat ttgctgtgtt ttactgcatt
     7141 gtagactgct tcctgaataa tatggtacac atgcgtcaat acttctgaat ttgaaatact
     7201 aatttcttta tcacatatat aggtacaggt aatcccataa atcattgcaa cagtttgtat
     7261 taattcatgc accagggcag ataaaccctg tttttctatg aaatatgatg gtgttaaccc
     7321 tcttgaaaat tggcgtgttt tgacaatggc accatctaaa agctgccgga tgttttccac
     7381 atcttcttta agcggcagta tattctttga taattcctgt atcatcacct tgcacagggc
     7441 gtctatcccc agcaaatgag gaataagatc atcatggagg tcctgaccaa tcgtctgtcg
     7501 ctccttttcg ctaatcaaaa ttatctcttt ttcaagcatt cgtttctctg taatatcagt
     7561 aagtgatata ataaccatct tctggtcatt aatatatatc acctctgccg ataaaattgc
     7621 gatgtattct tttgaatcat gtttcaatga aatttcataa tcactgattg tattaaactt
     7681 aatgagctgt ttatataatc gttgtatatc tcttttttta attattccaa tatcaaagat
     7741 gctaatagcc ggtttgtggt gcaatggcac atggataagt atctcaaaag ccttgttcca
     7801 ttttacaatc atccctgttt gcatatcaat aagcgccaat ccaacaggag ttgcatcaaa
     7861 tacttttgca aaaagctctt ctgactgttt taaagcctct tcccaatttt ttcgctgggt
     7921 tatatcagta actaccgcaa agctacccaa aaatcgtccc tgctcatcgg atactatttt
     7981 tggggataca atggtatgcg ctattgtccc atcatctttc atccatgcca tttcataggg
     8041 ttcctgaatt ccaaactgac gtttttccat ctggatatca aactggcaat agtaatgagg
     8101 atggataaaa tcttttattt gtttaccaat aatatattgt tgatcatatc ctacaatatc
     8161 acaaaatcgt ggattaacgt attgaattgt taaatgttca tcctgtacca gcaacccatc
     8221 actcattgtt tccacaagca aacggtattt ttcttcactt tttcgcagcg cttctcttgt
     8281 ttcaactaac tgggtaatat cggttaacga agcaacactc aattgagtac ctggaatcat
     8341 atcaatggta atataaatgt acagtatctg tttatctttt gtatatattc tgaattcata
     8401 atttttaggt gcactatccg gatcttttct tcgcaaacag tgatattctt tcattctttc
     8461 tacatcgtct tcatgtataa attgcgtcca gcttaccctc ccttcaatct cttgtttatc
     8521 atatcccagc atggaagcaa aacggctatt aaccagaata attgttgtat ctgcatctat
     8581 gaggatggtt cctgcacctg tattttcaaa tattgtgata tatctgtcat tgaatgccgg
     8641 ggtattgtta ctgtttgact ccattggcat gaactatcgc cccataaaat ttttttacaa
     8701 aaggattgta tagagtatca tgactttttc aacatgcatt tgatctctct tcaaacaccc
     8761 agtgcaaatt tttcggcaga tcgagataat cagcatgtgc agcctcttct gttaaatcaa
     8821 cggaattata ggtatgccag tacaatattg ttttgttgtt atattccggg ttttgtatca
     8881 taccacatac cgctgcaaat gttttaccgg tataggttgg ttctaacggt atattttcca
     8941 actggtgcat tgtgtcaatg gcatccatac atgcagcggt tgggtagcca tatccttcac
     9001 cacaataatc gtgtacgata gctggtgtca taaaatcaac tgctggcaat tgagtaaacc
     9061 ctgccatgag tgcataggtt tgcttcatca gtgacagtac actcttttca ttggcaatag
     9121 gcacaggtcc aagggatcta tcggttaccc tgaccccaat aaccttactg cgtaatcccg
     9181 ctaacgctgc acccagggta agccctgcca tagttcctgc cgagcccaat gcacacacaa
     9241 tataatctgg ctccagcata agcccctgct gtatctgctg ctgtagttca aacgcagcat
     9301 ttacaaaacc taccgttccc agtggcgagg aaccgccggc atacaaaaaa taggcatcgt
     9361 gcattaaccg ttcaaatgta taaaacataa cgccggcttt cagcatggtt ttgtaatatt
     9421 ttaactgtgc attatagcgg cacaataaca gtaaattttt ttttacatac gcggttaccg
     9481 gctggtaaaa caaaagaagc ctgcatgcaa gccccagctt ctggcaaaac atagcggttg
     9541 ccaatccatg gttggtgcca atgcccccca tggtaacaac ccttgattta ttcagagcct
     9601 ttgcctccgc tagcgtaaat tctaattttc ttaccttatt gccgccatat acctctgaag
     9661 atagatcatc ccgcttaatc catagattat catgagaaaa atgatgcagt cgttgtacag
     9721 gcgtcgggaa tttgccaagt gacagccatg gtaattgttg caacgcttcc catgtatcca
     9781 gcagcggtaa agttttcaca ggctacctcc agtcttcctt tgtggcgata gctactatgt
     9841 caaaaccctg ccttagcaga aatcgtttgc gtaaataata gtacgccaca ccactcaaag
     9901 caaacagtag aaataagcca atcttaagca gcgattgcag atctaccaga atgatgatgc
     9961 aaaaaaacac tatgaggata aaacccgcaa aagtacagag gtacaaccac acccctttta
    10021 atttaaaatg tgcctgcatg taacgctcag gatacagttt tggaaatcgc agcgctgcaa
    10081 taagaatagg aagaaagacg ataatgccac ccaatgctga gtacgatgca aacgttgcaa
    10141 tggaaaaatt tgaaaggata cctgcaagcg atagtaacca gataatggtt agcagtatat
    10201 gaggcgtggc aaatttttta tgaacatttc ccaaccatgc tggaagcagc ctatcattga
    10261 taataataag cagcgatttg gtgcctacaa taaacaaggc attgagcgtt gtgataaggg
    10321 caagcactgc accgcccagg ataaaaaaca taaacccggc actgtctagt attgatttgc
    10381 ttatgaacac taaaggctct tcagcctgtg atgttgtatg ccatggaaga gcacctgttg
    10441 cagcaatagc cacaagaagg tacactatgg caacaatggg aaaggtaata aaatatgccc
    10501 tgggtatagt tttccccgca tcttttattt caccacccaa ttcaataatc ccatttgatc
    10561 ctaaataggt aaaggtaagc agtgcaatcc ccactgcaag attcccaata cccttctgaa
    10621 aaaaattttc aaaattttta ccatcaagat gaacaattcc agcaaatgaa aaaaacagca
    10681 aggcgcttgc caggatgata accatgattg cctgcaaaac ggaagcaggt ttaatcccga
    10741 taagatttac caggtaaaat actgttacaa gaactatcgc catacattgc acagacatag
    10801 cagggaaaaa agtagctatg taccgcgcgc aggatatgct gtacagtggg atctgtccaa
    10861 aaaatgttgc aagtacataa atccatatgc cagcaaaggc aacacccggt gataccatac
    10921 ggctaggata tttataattg ccaccaactg acggaattgc cgaccccagc attgcaagtg
    10981 gaagcatgct taaaaagacg ggaatcaccg ccagcaaata ggcaagcggt aatgcaggtc
    11041 ccacaatttt gagtacaatg cccgtaaaaa caaaaatacc accaccaatg gtaaatccca
    11101 ctgcaataaa ggtaacctcc gcaagtccca gtacccgttt taagttcatg gttttattgc
    11161 tgttcattgc tttttcacca tctattgtat actatgtttg ctcatcactg aacaatcatt
    11221 tgatgccact ttgtatggac tttgttatat tttcaaggat gtcactataa acatttttaa
    11281 tcctttcaaa gatttctacg gatgttattt catcataaat atgtgcgctc acattcctat
    11341 cctccagcta tccaatcact ccctcttata gtgttttaat cgtctccaca aaagaattgc
    11401 ttatctccca ttttgaaagc cagcaccggc gtttatactg caataatcat acaagcaata
    11461 cataggcatt gtcaagataa atacagagtg ttaataaaaa cttgttcttt cacgggcagt
    11521 aaaaaaacat atagaatata aagtgttggt ttacgccaat aaaacgatag tattt
//
LOCUS       DAOSOX010000068        11571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5557_length_11571_cov_8.509986, whole genome shotgun sequence.
ACCESSION   DAOSOX010000068 DAOSOX010000000
VERSION     DAOSOX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_11571_cov_8.509986"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1588)
                     /locus_tag="PLX22_06435"
     CDS             complement(161..1588)
                     /locus_tag="PLX22_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT19572.1"
                     /translation="MSKKFIALLIVVIAIAGFVIYRAFSPAKVDTAISYIDDGRYAKA
                     LRVLDSLSKTAHYEEGERIYYYRLKALLGFINELNSDYEEERETIDKSKDNKLIDKLK
                     RDIEKINKKEGLDCILVTEPKCYIFTAGKPYDEFIALYPGSRYRESIDFEILQFMIGV
                     HVNTIDPIIEFYKRYPETTYLSSLVNILFKALASPSITVKEHTETLSSMLEKFCNKYS
                     SSAQYYRIFRCKGENVNLRDSSGTFGQITGKLDKGEIVVQLERSMDSVQIGDVRDYWY
                     RVVSVSGSRGWVFGKYLEQVPVKQAQAVEEEKFTIDERFATWIDSNTPEGWQHYFHGY
                     ESAISFVKSSNQNIVKVNTQDKGGLYKKTGSFQNITCKVKGRLIRGNVILLGVVGYGG
                     YAYVITLMPKSLDACGYHIPYDTSLWHEYEMRSEGKIFSLYIDGELVARKISPVKHPL
                     LTSAGIFILVCQDGGSAEIEYVKVK"
     gene            complement(1596..2807)
                     /locus_tag="PLX22_06440"
     CDS             complement(1596..2807)
                     /locus_tag="PLX22_06440"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOT19573.1"
                     /translation="MVNRKIAILVGDGMADYSIGELGDKTPLQYADTRHMDYLAKNGI
                     CGLAHTVPQGMPPGSDVANLSLFGYDPVQCYTGRAPLEALNMGIELGPDDVAFRCNIV
                     TIDNSIMQDFSADHIDSALTKIVIDELAQQIQLKDIEFYAGVSYRNIVVIRHFTGDMP
                     VTTPPHDIQGQAVNDYLPMSDNSKLLNDIMKQASEVIAHSKVIAEAKTRYRGNPTAIW
                     LWGGGFKPSMEPLSKRFGLYGFTISAVDLIHGIGRAAGLSPIYVEGATGYIDTNYDGK
                     AAALIEAMNKANFVFLHVEAPDESGHEGNLEHKLKAIEDFDAKIVGPVVNMLNRYGEF
                     TVLVMPDHPTPLNLRTHTSEPVPFVIYSPDMNYRLQCNGFNEVDAKATGIVVDRAHTL
                     LAMMVNNSFPW"
     gene            complement(2800..3558)
                     /locus_tag="PLX22_06445"
     CDS             complement(2800..3558)
                     /locus_tag="PLX22_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOT19574.1"
                     /translation="MIIALLTDFGHRDPYVGMVKAVLYRHGFNGFIVDICHDIVPYSI
                     EHAQFMLYTTYSFFPAHTIIMSVIDPGVGTQRNILLATDGIYYFLAPDNGLLSCIKNL
                     VDVWAVETEAFKNASSTFHARDIFAVIAARLATSGIDAIAKRQVHEYIHTPFPYFCVN
                     NGAILCKVMHIDRFGNCILSLPNDRYPVPLQKAVVHDRDLMVHHCKTYADLPNGAVGL
                     LQGSSGFVELCMRENDCSGTLGLHIGDGVTLVYG"
     gene            3959..4360
                     /locus_tag="PLX22_06450"
     CDS             3959..4360
                     /locus_tag="PLX22_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT19575.1"
                     /translation="METTLDKVQFRLTQSREKLLLASIFLEKKKYCDSILSSYRAIFY
                     AIRVLLVGKDIDSDDPDTIIEIFEKYLDSTVFRNAEIIEIAKRSKEIKNYVAHSNNQI
                     THDNAETMLNNASTILHEVEKYYNHYNFNSH"
     gene            complement(4388..4678)
                     /locus_tag="PLX22_06455"
     CDS             complement(4388..4678)
                     /locus_tag="PLX22_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19576.1"
                     /translation="MPSRSNVKKSPSKGKRKTNLLKLCTAMNKLAMDAVDKEVVKRFK
                     TVIDSATEDITKSLADQVINNSDELQINVIPEMFHPYVKHFLFMKKRNSKIK"
     gene            5104..5235
                     /locus_tag="PLX22_06460"
     CDS             5104..5235
                     /locus_tag="PLX22_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19577.1"
                     /translation="MTCDKCGAEINRLNHYYIPGDKNTKGKRYCIRCAKEEKIVTLI"
     gene            5342..6445
                     /locus_tag="PLX22_06465"
     CDS             5342..6445
                     /locus_tag="PLX22_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOT19578.1"
                     /translation="MENDQISILIVDDEEKICNRLDAVLQRQGYYTVATTQSEHAIEY
                     LSQQPFDIIITDLNMPQNSGFQIMEYIQQAGINILIIVITGYASVDTAIQSIKLGAYD
                     FIQKPFDIETLKLTVKRAALTVKLRKENERYVEELKKLDNLKNEFISIVSHDLRSPLA
                     TIGAYVKYLTKKEDFPEKYVRYLYIIGEIAENLYNLVNELLDVSKIERGILHLNYEEV
                     DINTVITSTIETFSTLAKEKNNDIIYIQKPENALIRIDKIKIIQVLNNLISNAIKFTE
                     NGTITIKARSSEDGISIVIQDTGIGIDDELKKSILDPYTLLHTNGTRGEIGTGLGLTI
                     CKRFVELHGGSISVESEKDKGSIFTIFLPYTIT"
     gene            6463..7560
                     /locus_tag="PLX22_06470"
     CDS             6463..7560
                     /locus_tag="PLX22_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19579.1"
                     /translation="MAKQFLKLSFSKRYPLFIIVTISSCCIAVIIVTINLISSMYTTS
                     VDYSKQLSIQHIESMSMVLHKLISETIVKPDTTHDTNIILYKKKLSQLVEQINAIATF
                     YIAKPTDNDRNYIVVETIKAAPLQGKIITDHSANFIMRKGLLRVTVNPSIKDDPYSYI
                     ESYMPLKYNNKNYIIIIHWLVPHISILQNKHAADVQSLIQIIAIIILVTVFIITFLSI
                     IHFMRTRSLLQELSHSIQSIANGNMDVMLNDSIDNDLKTIAVSFNSLAQEFKNKDEAL
                     RKIKEETFADIFKQGVQRLKENQLDEAIACFITVTIFKPKSFASYFNLGVCCAKKGEL
                     NKSVEYFTQAQEINPDNQLTMRYIEKIRGLQ"
     gene            7590..9374
                     /gene="uvrC"
                     /locus_tag="PLX22_06475"
     CDS             7590..9374
                     /gene="uvrC"
                     /locus_tag="PLX22_06475"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT19580.1"
                     /translation="MQKVKNLPSKPGVYLFSDAKNEIIYIGKASSLKKRVSSYFKMHV
                     DAKTNVLVSSIHGIDYIITQSEIEALLLESSLIKKHRPKYNIQQKDDKRFPYIAITLH
                     EQFPRIIFTRKILYNGDKYYGPFTDAQAARKTVSLINKTFQLKTCNKEIPLKPGQRPC
                     LNQQIGRCQGVCTGIVSQAEYRNVIHTVMQFLDGNIEPVVENLTALMNTYSNSMRFEI
                     AAQIRDMIYNIQTIAQEQHVVTPLGQNQDYIGLTAHQDEAFILVFQFRHGSLIGRRIF
                     VFTNAEYTRTSTFIAQFIVQYYASNEPPQSIIIPEATDDKQILEQYCLEKATQPVTIA
                     LPRTSHEQAVMKLIQKNLDLLVAQKIAPDQKKALFELQQVLALPVLPVTIECFDISNI
                     QGQFAVASMVHFTDGMPNKKEYRRYRIRAYLQPNDPGMIHEVVGRRIQHCINEKLPLP
                     DLLVIDGGKAQLSRALEVLNAFECSIPVIALAKRYEEIYTPHGHPLRLPVTSPALKLL
                     QTIRDEAHRFAITYHRNLRDKNLTASILDEIPGVGMQYKKKLLQNFATIQVIKQSDIE
                     TISSQAKIPKSVAQKVYHFFNAQSEKKQ"
     gene            complement(9428..10054)
                     /locus_tag="PLX22_06480"
     CDS             complement(9428..10054)
                     /locus_tag="PLX22_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOT19581.1"
                     /translation="MSDASFIPTVFFTLFFVVDPLGLIPVFVAYLAPYDAAYRKKVII
                     KATVISIGVSLFFIFSAHVFLRFIGITPGSFMIAGGILLFVIAMDMLLARPRRTQQFD
                     DKEEYNFDTDIAVFPLAIPFLCGPGNIAALLMFSSQAQGDIVKLGIISIISIATFLIA
                     MLVMAIALPLQTLLGRTGISVISKLMGLILSAMAVQFIYNGLVQMGVR"
     gene            complement(10057..>11571)
                     /gene="serA"
                     /locus_tag="PLX22_06485"
     CDS             complement(10057..>11571)
                     /gene="serA"
                     /locus_tag="PLX22_06485"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOT19582.1"
                     /translation="DFDIVYKPGLKADDLKKEITNAQALIIRSATHVTKDIIDAGAKL
                     EIIARAGIGVDNVDVESATAKGIIVTNAPGGNTVSTAEFAITLICALARNIPQAYASM
                     KQQKWERQKYEGTELRRKTIGIIGLGRVGSELAKLCKGLGMNVVGFDPYVAKEKLTAL
                     DIDIVDLDTIWKKADFISVHTPFTDETNNLINADVIKKLNPGVFIINCARGGIVNEQD
                     LYNALKEKRIAGAALDVYTQEPPDNLPPFHELNNVILTPHLGAYTYEAQVSVAIEAAQ
                     TIVDYFTRSVVQNSVNFASVDMNQYNFMKPFINCMERMGRFQAIMLDGKISSVQIYYC
                     GEFGNFSKDPLTAAYLKGLLSPFIDRPLNYVNATFIAAERGIKVHSGESTEAGDWAHL
                     IQVKAEGALKSELWGTVIAKQPWVVRFDEYLIDFIPSGRMLVMHNNDVPNVIGVIGTF
                     LGQHHINIANLHLARTRRGGKALVILEIDEDVPQDVLTMLSRLPEILDVRYVAV"
BASE COUNT         3815 a         2436 c         1928 g         3392 t
ORIGIN      
        1 gcaataacta tgagggcaat gggtagtatg atgcaatact caacaagggt ggttgcaaga
       61 taaaaacgcc ttacaaacgc tcgtattgct ctctccatct gatgcctcca tactttctat
      121 ctataaacaa ataaaattac tattatctgc agtttgtcaa ttatttaacc tttacatatt
      181 caatctctgc cgaaccacca tcctgacaaa ctaaaataaa aattcctgct gacgttaata
      241 ggggatgttt taccggtgaa atcttccttg ccacaagctc accgtcaata tacaatgaaa
      301 atatcttccc ttccgagcgc atttcatatt catgccacaa tgaagtatca tacggtatgt
      361 gataaccgca tgcatccaaa ctttttggca taagtgttat aacatatgca tatccgccat
      421 atccaacaac accaagcagg atgacattac cccgaataag cctgccttta accttacagg
      481 ttatattctg aaagcttcct gtttttttat aaagccctcc tttgtcctgt gtgtttacct
      541 ttacaatatt ttgattactg ctttttacaa aacttattgc tgattcatac ccatgaaaat
      601 aatgttgcca cccttcaggc gtatttgaat caatccatgt agcaaaacgc tcatcaatag
      661 taaacttttc ttcttcaacc gcttgtgcct gctttaccgg tacttgctca agatatttac
      721 caaaaaccca ccctctgcta ccacttaccg atacaaccct gtaccagtaa tccctgacat
      781 cgccaatctg tactgaatcc atggaacgct caagctgtac tactatctca cctttatcaa
      841 gcttgccggt tatttgtcca aatgttcccg atgaatctct caggtttaca ttttcgcctt
      901 tacacctgaa tatgcgatag tactgagcac ttgaggaata tttattgcaa aatttttcaa
      961 gcattgaaga tagtgtttct gtgtgctcct ttactgtgat ggaaggactt gccaatgcct
     1021 taaaaagaat gtttactagc gatgacagat aggtggtttc cggatagcgt ttataaaact
     1081 caataatggg atctatagta ttgacatgaa caccaatcat aaattgcaaa atttcaaaat
     1141 caatgctttc acggtagcga cttcccgggt ataaagcaat aaactcatca tacggcttcc
     1201 cggcagtaaa aatataacat ttcggctccg tcaccagaat acaatccaat ccttcttttt
     1261 tatttatttt ttctatatca cgttttagct tatctatgag cttgttatcc ttgcttttat
     1321 caatggtttc acgctcttct tcataatcac tattcaactc attaataaat cccagcaacg
     1381 ccttcaaacg gtaatagtaa atcctttcac cttcttcata atgagctgtt tttgacaaac
     1441 tatcaagcac tcgaagggct ttagcatatc tgccatcatc aatatacgaa attgccgtat
     1501 caacttttgc cggggaaaaa gcacgatata taacaaatcc agcaatagct ataactacaa
     1561 tgagtaaagc aataaacttt tttgacatag tgtcctcacc atggaaatga attgttcacc
     1621 atcatcgcca gcagcgtatg tgcacggtca accactatac cggtggcttt cgcatccacc
     1681 tcattaaagc cattgcactg caaacgatag ttcatatctg gcgaatagat gacaaaagga
     1741 acaggctcgc ttgtatgagt ccttagattc agtggcgttg gatgatcagg cataaccagt
     1801 acggtaaatt cgccatagcg attaagcata ttgacaacag ggccaactat ctttgcatca
     1861 aaatcttcta tagctttcag cttgtgttca agattaccct catgccccga ctcatcaggt
     1921 gcttctacat gcaaaaacac aaagttggct ttattcatgg cttcaatcaa ggctgcggct
     1981 ttaccatcat aattagtatc tatatagccg gttgcacctt caacatatat gggcgatagt
     2041 cctgctgctc tcccaatacc atgaatcaaa tcaactgctg atatggtaaa cccgtataac
     2101 ccaaatcgct ttgacagagg ttccatgctg ggtttaaatc caccacccca tagccatatt
     2161 gccgtgggat ttcccctgta tcgggtttta gcctcagcaa taacctttga atgagctatc
     2221 acttcagatg cttgtttcat tatatcattg agcagcttgc tgttatcact cataggaaga
     2281 taatcattaa cagcctggcc ctgtatatca tgaggcggcg tggttactgg catatctcct
     2341 gtaaaatgcc gtatcaccac aatattacgg tatgaaaccc ctgcataaaa ttctatgtct
     2401 tttaattgta tttgctgtgc aagctcatca ataacaattt ttgttaatgc tgaatcaata
     2461 tgatcagcgc taaaatcctg cataatgcta ttgtctatag tcactatatt gcaccggaat
     2521 gcaacatcat ctggaccaag ctcaatcccc atattcagcg cttcaagcgg tgccctgccg
     2581 gtatagcact gtactgggtc atatccaaac aatgaaagat tggcaacatc acttccagga
     2641 ggcatcccct gcggtactgt atgtgccaat ccgcaaatac cgttttttgc cagatagtcc
     2701 atgtgcctgg tgtcggcata ctggagaggt gttttatccc ccagttcgcc aatggaataa
     2761 tctgccatac catcacccac cagaattgct attttgcgat taaccataaa ccaatgttac
     2821 tccatcccca atatgtaatc ccaatgtacc cgagcaatcg ttctcacgca tacatagttc
     2881 aacaaagcca ctgctgccct gcaataagcc tacggcacca ttgggcaaat ccgcataggt
     2941 tttgcagtga tgcaccatga gatctctatc atgtaccact gccttttgca agggtaccgg
     3001 gtatctatca tttggcaatg ataaaatgca attaccaaac ctatctatat gcattacctt
     3061 acacagtata gcaccattat ttacacaaaa ataaggaaag ggagtgtgaa tatactcatg
     3121 cacctgcctc tttgctattg catcaatacc actggtagca agccgtgcag ctatcacagc
     3181 aaagatatca cgcgcatgaa atgtgcttga agcatttttg aacgcttccg tttcaactgc
     3241 ccatacatcc accaaatttt ttatgcacga tagtaaccca ttatcaggtg caaggaaata
     3301 atagatacca tcagtagcta ataaaatatt tcgctgtgtt ccaacgccag gatctataac
     3361 cgacataata atagtatgag cggggaaaaa gctataggtt gtatacagca taaactgggc
     3421 atgctctatt gaataaggga caatatcatg acagatatca acaataaagc cattaaaccc
     3481 atgtctgtat aacactgcct tcaccattcc cacatagggg tcacgatgac cgaaatcagt
     3541 taacagggca ataatcatag taactattgt aaaatattaa tattatacct gtcatacaaa
     3601 taaactacta cacaatcaaa tattttttta taaaaaacta tcggtacagc acatggaaaa
     3661 atactagaac cgtatataga taattactgc caactataat cctatagtta aatcccttaa
     3721 aaaattatga tggtattgcg aatataatta tatataaagc ataaaaaaac atctaaccct
     3781 ttaatttgtt actatcactg gatacaataa caactgaata accattgtat acataaaatt
     3841 gcttcaaaaa tgaaaaataa gttttgcagg ttatgataaa aaagacttgc aataatacgc
     3901 tgatatcact atatttaagc aagcgacgtg aacatcatta aaaataattt tattctatat
     3961 ggaaaccaca ttagacaaag tacagttcag actaacgcaa agcagggaaa agcttttatt
     4021 ggccagcatt ttccttgaga agaaaaaata ttgcgattca atactatcct catacagagc
     4081 aattttttat gcaatacgag tgttgctggt gggcaaggat atagattcag atgatcctga
     4141 taccattata gaaatttttg aaaaatacct tgattcaacc gtttttcgta atgctgaaat
     4201 aattgaaatt gcaaaacgat caaaagagat aaaaaattat gttgctcatt ccaataatca
     4261 aattacccat gataatgctg aaacaatgct gaacaatgca tcaactattt tacatgaagt
     4321 tgaaaagtat tataatcatt acaattttaa cagccactaa cggttgaaat aaacacattc
     4381 ttccattcta ttttattttg ctgttacgct ttttcataaa aagaaaatgt ttaacataag
     4441 gatgaaacat ctcgggaata acgttgatct gcaattcatc tgaattatta ataacctgat
     4501 ccgctaaact ttttgttata tcttcagtag cagaatctat aacagtttta aaccgtttta
     4561 ccacctcttt gtcaacggca tccatagcta atttattcat tgccgtacac aattttaata
     4621 gattagtctt tcttttaccc tttgatggtg atttttttac attagatctc gatggcatta
     4681 tacaataaac cccccaaaat aatcatcaat gctaaaaata attgtacata atgtaaacat
     4741 aattttcaaa aaaaatttcc atgcactatt gtatcagact gtgtataagc atgattgccg
     4801 ataccatagt accatataat gcaataatcc aaaaaaaatt tttaatactt atatgacaac
     4861 aaaaccatag ttcaacacct tgcccggctg ttgataagca aaaaatggtt ttataactga
     4921 ttataaaaaa tataataaaa agacttttat ttaccgacaa ttatcatgca tgaacctctg
     4981 gtaattattt tatggatgtt ccatgtcagg cacatgcatt ggaaaaatgg tttgttattt
     5041 ttccaaataa tgaaaaattg atttttaggg tgtctataaa tcttttatgc aggagtatat
     5101 catatgacat gtgataaatg cggcgcagag ataaacaggc ttaaccacta ctacatacct
     5161 ggcgataaaa ataccaaagg taaacgttac tgcatccgat gcgctaaaga agaaaaaata
     5221 gttacactta tttagataag cactaaaagt tttactaaaa aatgacattt atagtacagg
     5281 gtcattgtag caacaacaaa taacttgaca tggttataga tgccatgcca taatcctgtg
     5341 tatggaaaat gatcaaattt ctattcttat tgttgatgat gaagaaaaaa tctgcaatcg
     5401 tttagatgca gttttgcaac ggcaaggcta ttatactgta gctaccacac aaagcgaaca
     5461 tgctattgaa tatctttcac aacaaccatt tgatataatt attactgatt taaatatgcc
     5521 acaaaatagc ggtttccaga taatggagta tattcagcag gcgggcatta atatacttat
     5581 tattgttata actggctatg catcggtgga tactgcaata caatccatta aattaggtgc
     5641 ttacgatttt attcaaaaac catttgatat tgaaacacta aaattaaccg taaagcgtgc
     5701 agccctgact gtaaaactaa gaaaagaaaa tgaacggtat gtagaagaac tcaaaaaact
     5761 cgataacctg aaaaacgaat ttatctcgat tgtttcccac gacctccgct cacccttagc
     5821 aactattggt gcctatgtaa aatatcttac caaaaaagaa gattttcctg aaaaatatgt
     5881 gcgctatctc tatatcatag gagagattgc tgaaaattta tataatctgg tcaatgaact
     5941 actggatgtc tccaaaatag aacgcggtat tttacattta aactatgaag aagttgatat
     6001 caatactgtt atcacatcaa caatcgaaac cttttcaaca ctggcaaaag aaaaaaacaa
     6061 tgatatcata tatatacaaa aacctgagaa cgcattgatt agaatcgata agataaagat
     6121 aatccaggtt cttaataatc ttataagcaa tgcaatcaag tttaccgaaa atggcaccat
     6181 aaccataaag gcaaggtcaa gtgaagatgg tatatcaatt gttatacagg atacaggtat
     6241 tggcatagat gatgagctta aaaaaagcat attggatccc tatactctgc tgcacaccaa
     6301 tggaacacgg ggcgaaatag gaacaggact tggattaact atatgcaaac gttttgttga
     6361 acttcatgga ggttccatct cagttgaaag tgaaaaagat aaaggcagta tatttactat
     6421 ttttcttcct tatacaatta cataaccttg ctgaggctga ccatggcaaa gcaatttctt
     6481 aaacttagtt tttcaaaacg atatccgctt tttatcattg tcaccatctc ttcatgctgt
     6541 attgctgtaa taattgtaac tatcaactta atctcttcta tgtataccac atcagttgac
     6601 tattcaaaac aactatccat tcagcatata gagtctatga gcatggtgct gcacaaactg
     6661 atatcagaaa ctatcgttaa acctgatact acgcatgata ccaatattat actgtataaa
     6721 aaaaaactat ctcaacttgt tgaacaaatc aatgcaatag ctacatttta tattgcaaaa
     6781 ccaactgata atgacagaaa ctatatcgtg gttgaaacta taaaggctgc accattacag
     6841 ggtaaaatca ttactgacca ttcagccaat ttcatcatgc gaaaagggct gctaagggtt
     6901 accgttaatc catcaatcaa agacgaccct tacagctata ttgaaagcta tatgccgctc
     6961 aagtataaca acaagaatta tatcattatt attcactggc tggttcccca tataagcata
     7021 ttgcaaaaca aacatgctgc cgacgtgcaa tcccttatcc agataattgc aatcatcatt
     7081 cttgtcacag ttttcattat taccttttta tccataattc attttatgcg cacacggtcc
     7141 cttttacagg aactatcgca cagcattcaa agtattgcaa atgggaatat ggacgtgatg
     7201 ttaaatgaca gcattgacaa tgatcttaag actattgctg tttctttcaa ttcacttgca
     7261 caggaattta aaaataagga tgaagcctta agaaaaataa aagaagaaac atttgcagat
     7321 atctttaaac aaggggtgca gcgtttaaaa gaaaatcaac ttgatgaagc catagcatgt
     7381 tttatcacag taactatatt taaaccaaaa agctttgcaa gctactttaa tttaggtgtg
     7441 tgttgtgcaa aaaaaggtga acttaataaa tcagtagaat actttacaca agctcaagaa
     7501 attaatcctg acaatcagct aaccatgcgc tatattgaaa aaatacgagg cttacaataa
     7561 ggtatagcag cacaatgtat gagcagttag tgcaaaaagt taaaaacctg ccctcaaagc
     7621 cgggggtgta tcttttcagt gatgcaaaaa atgagatcat ttatattggc aaagcgtcat
     7681 cgttaaaaaa aagagtttct tcatatttca agatgcacgt tgacgcaaag acaaatgtgc
     7741 ttgtttccag catccatggt attgattata ttataacaca aagcgagatt gaagcactgc
     7801 ttttagaaag ctcacttata aaaaaacata ggccaaaata taatattcag caaaaagacg
     7861 acaagcgatt tccttacatt gctatcacac tgcatgaaca atttccacgt attatattta
     7921 cccgtaaaat actatataat ggcgataaat attatggacc attcaccgat gctcaggctg
     7981 cacgcaaaac ggtttcgctt atcaataaaa catttcagtt gaaaacatgc aacaaagaga
     8041 taccgttaaa accaggacag cgcccatgcc tcaatcaaca gattggcaga tgccaggggg
     8101 tatgcaccgg tatcgtttcc caggctgaat atcgtaatgt tatacacact gtgatgcaat
     8161 ttttagatgg gaatattgag ccggtggttg aaaatctcac cgctcttatg aatacatatt
     8221 caaactccat gcgctttgag attgcagcac agatccgtga tatgatatac aatattcaga
     8281 caatagcaca agaacagcat gtggtaactc ccctggggca aaaccaggat tacattggtt
     8341 taaccgcaca tcaggatgag gcatttattc ttgtatttca gttcaggcat ggaagtctta
     8401 ttgggaggcg tatatttgtc tttaccaatg cagagtatac cagaacatcg actttcattg
     8461 cacagtttat tgtacagtac tatgccagca atgaacctcc acaatcaatt attatccctg
     8521 aagcaacaga tgataaacaa atccttgaac agtactgctt agaaaaagca acacaaccgg
     8581 taaccattgc actgcccaga accagtcatg aacaggcggt tatgaagcta atacagaaaa
     8641 atttagatct tctcgttgct caaaagattg ctccagacca gaaaaaagcc ctttttgagc
     8701 tgcaacaggt gcttgccctc cctgtactgc ctgtaacgat agaatgtttt gatatatcaa
     8761 atattcaggg tcaatttgcc gttgcatcca tggtgcattt tactgatggc atgcccaata
     8821 aaaaagagta tagacgatac cgaattcgtg cttacttgca gcccaatgac cccggcatga
     8881 ttcatgaggt tgtgggcagg aggatacagc attgcataaa cgaaaaactg ccgttgcccg
     8941 atttgctcgt tattgatggt ggcaaagctc agctttcgcg cgcccttgag gtactcaacg
     9001 catttgaatg ttctataccg gtgattgctc ttgcaaaacg ttatgaagaa atatatactc
     9061 cacacggtca tcccctgcgc cttcccgtta catcgccagc attaaagcta ttgcaaacaa
     9121 tacgcgatga agctcaccgc tttgccatta cctatcatcg gaatcttcgc gataaaaatc
     9181 ttactgcatc aattttggat gaaatacctg gcgttggtat gcagtacaaa aaaaagttgc
     9241 tccagaactt tgcaactatc caggttataa aacaatcaga tatagaaact atttcatcgc
     9301 aggcaaaaat cccaaaaagt gtggctcaaa aggtatatca tttttttaat gcgcagagtg
     9361 agaagaagca ataacaggtg tttttattgt atggtgcgat agttactata tatcaatata
     9421 tcaataacta tcgtacaccc atctgtacca gcccgttata aataaattgt accgccatag
     9481 ccgacaggat aagtcccatc aacttgctga ttaccgagat accggtgcgc cccagcagtg
     9541 tctgcaatgg taaagcgata gccattacca gcatcgctat caaaaaagta gcgatagaaa
     9601 ttattgaaat aatgccaagc tttactatgt caccctgtgc ttgagagctg aacatcaaaa
     9661 gagccgcaat attgccggga ccgcataaaa aaggaatggc aagcggaaaa acggcaatgt
     9721 cggtatcaaa attatattct tctttatcat caaactgttg tgtgcggcga ggacgtgcaa
     9781 gcagcatatc catagcaata acaaacaaca gtattccccc ggctatcata aatgaaccgg
     9841 gtgtaatacc tataaagcgc aagaatacgt gtgcactgaa tatgaaaaat aacgatacac
     9901 caatagatat taccgttgcc ttgattatga ctttcttacg gtatgcagca tcatacggtg
     9961 ccagataagc aacaaaaact ggaattaacc ccagagggtc caccacaaaa aataaagtaa
    10021 aaaaaactgt agggataaag cttgcatcac tcatggttac accgcaacat atcgcacatc
    10081 caatatctcg ggcagccttg ataacatggt cagcacatct tgcggcacgt cctcatctat
    10141 ctctaaaata accagtgcct taccgccacg ccgcgtccgc gccagatgaa ggtttgcaat
    10201 attgatatgg tgctgtccca gaaacgttcc aataactcca atgacattgg ggacatcatt
    10261 attgtgcatc acaagcattc tgcctgacgg aataaaatca atgaggtatt catcaaacct
    10321 aaccacccat ggttgttttg caatcaccgt accccatagc tcacttttta atgctccttc
    10381 tgctttcacc tgtatgagat gcgcccaatc acccgcttcg gtactttctc cgctgtgcac
    10441 tttaatgcca cgctctgctg caataaacgt tgcattaaca taatttaaag gcctatcaat
    10501 aaagggcgat agtaaccctt taaggtaagc tgccgttaaa ggatccttgc tgaaattacc
    10561 aaattcacca cagtagtata tttgtacaga agagattttg ccgtctaaca ttattgcctg
    10621 gaatctgccc atacgctcca tacaattaat aaacggcttc atgaaattgt actgattcat
    10681 atcaaccgat gcaaagttta cactattttg cacaacactc ctggtgaagt aatcaacaat
    10741 tgtttgggca gcttctattg ctactgaaac ctgggcttca taggtataag ctcctaaatg
    10801 aggtgtgagg attacattgt taagttcatg gaatggtggc aggttgtcag gcggttcctg
    10861 tgtgtaaaca tcaagcgcag ctccagcaat acgtttttct tttaatgcat tatataaatc
    10921 ctgctcgttc actattccac cgcgagcaca attaatgatg aacacacctg gattaagttt
    10981 ttttataaca tctgcattaa tcaaattgtt tgtttcatcg gtaaatggtg tgtgtacact
    11041 gatgaaatca gcctttttcc agattgtatc aagatcaact atatcaatat ccagtgctgt
    11101 cagcttctcc tttgctacat aaggatcaaa tcccacaaca ttcatgccca atcccttgca
    11161 caatttagca agctctgacc ccacacgtcc caatccaata atccctatgg tttttcttcg
    11221 cagttctgtc ccttcatatt tttgtctttc ccatttttgc tgtttcattg aagcatacgc
    11281 ctgcggtata ttacgcgcaa gtgcacagat aagggtaatt gcaaattcag cggttgaaac
    11341 ggtgttccca cccggtgcat tggtaacaat aataccttta gccgttgcac tttctacatc
    11401 aacattatcc acacctatac cggctcgtgc tattatctcc aattttgcac cagcatcaat
    11461 gatgtccttt gtaacatgcg ttgccgaacg gataattaaa gcctgtgcat tcgttatctc
    11521 cttttttaaa tcatcagctt ttaatccagg tttatacact atatcaaaat c
//
LOCUS       DAOSOX010000069        11484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5613_length_11484_cov_6.736372, whole genome shotgun sequence.
ACCESSION   DAOSOX010000069 DAOSOX010000000
VERSION     DAOSOX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_11484_cov_6.736372"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..645)
                     /gene="csm5"
                     /locus_tag="PLX22_06490"
     CDS             complement(46..645)
                     /gene="csm5"
                     /locus_tag="PLX22_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOT19583.1"
                     /translation="MEQYRATINIITPLIIHNGTFYDLWDIMPNPKNDEVFIIDVVRA
                     FMLMRENEKENFNTLLDEIVKKNNENDIKVLMTKLKSMLHNKVYEEPDSIIRKAKALE
                     GFTKQISDNPNLQINKIFTDKLTNKPFIPGSSVKGMLRTAMLESLRKKFKLEPKVERD
                     DVRKDKVVIKKALNFEARIYGSPENKHVVNLDRACPKIC"
     gene            complement(661..1590)
                     /gene="csm4"
                     /locus_tag="PLX22_06495"
     CDS             complement(661..1590)
                     /gene="csm4"
                     /locus_tag="PLX22_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOT19584.1"
                     /translation="MASVYKVVCQPMSFYSMMYKADTIFGAFCWSIRYFEGADALEGL
                     LEKFKSSPPFLLSSMMPYGYMFKPLLHPPRIESNNKSEAKGLLEKEKKRKKIQFVTID
                     FISKNQKNLSQAIVSDELFNNIFDSTKIGRLEITRNAIDRITLHAREGALFSEVFLYS
                     EMPLVFYIKVNDEQYSEGWFASIVEAVGLNGLGNDCSIGKGVFKMKLEKITQQEIAMF
                     DYFSDNYVTLSVCIGADLYPRCYTTFTRYGKLGGLYSQCGINGSLVYCKKPVVFYHEG
                     SVFSGNGLGSMVNNVHQEKSIVQYGYAFPLYFG"
     gene            complement(1594..2232)
                     /gene="csm3"
                     /locus_tag="PLX22_06500"
     CDS             complement(1594..2232)
                     /gene="csm3"
                     /locus_tag="PLX22_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOT19585.1"
                     /translation="MAQLKEIKIVKGKILVETGLHIGANAKGVGIGELDNPVLKDVRT
                     GFPYIPGSSLKGKIRSLLELEHGISSNDKSKPCSCGKCPVCKVFGTTAKESNEITRVL
                     FRDAFLSEESKKELYDKNLQATEEKTENMINRLGGHAESPRTMERVIPGLTFDFEIAL
                     RVFDNDNNVAIDLLKKGLLMLQKDALGGSVSRGYGKIRFENVSINGQPFKLE"
     gene            complement(2233..2655)
                     /gene="csm2"
                     /locus_tag="PLX22_06505"
     CDS             complement(2233..2655)
                     /gene="csm2"
                     /locus_tag="PLX22_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOT19586.1"
                     /translation="MIEIDEKYLTTEPEAKAKIFKDRGLETSQLRKYYDELKVLEKRG
                     YGKSNDDFQKQILPMVKFMKAKIAYGAGRKVNNKRLVPVEFKDHMSAQIDKINDFNSL
                     RNFLLHYQAIICYFTFLNEFSQSQQQQQHQHQHQQRRG"
     gene            complement(2652..5069)
                     /gene="cas10"
                     /locus_tag="PLX22_06510"
     CDS             complement(2652..5069)
                     /gene="cas10"
                     /locus_tag="PLX22_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOT19587.1"
                     /translation="MDENLKHQLHILFASLFHDIGKFKQRAFGDKPHSNFPSAMDAMI
                     LPSTESGYGYKHALWTFDFFENDCKTVQLPKDLDWNEIRDMASKHHKPSNYKEEIIQL
                     ADRVSSSLDRSGEDEHFKHGIHLKKHLRSIFPQVSIGDKHKNLNHSIYKIARLNVNSV
                     YPVALQQEDDTLESQYKKLWDEFIDSFHQICLQWGNSNNIIQFAHCINYLLKKYTWCI
                     PAATNDIMCDSSLYDHAVSTAAIALTLYNYNSEQLPSKDEKAIVLFAADISGIQQFIF
                     QHHQQSFKGSSKIIRGRSFYISVIGLAYQYKLYKESGIAPFTQLMSSSGKFVMLLPNS
                     EKVLSILDKVQHEIDQWLFKEFHGDFAIITDNSVKASINDFGHDKFSSLIGQINYNLS
                     IAKYSKFKNILSGGNCVIDINYSEEEMCPACGKYSKNINYSDELGQRCDFCNTMFTLG
                     GKIVKKPLYLVFHDNAHNADSSFFNGTFSISFANDILPYRNAYAIYTLDESVETLPLL
                     ELNNYVPIVNNEIKTFEEIALSSVIHYNEDEENTMRGVPLLSFIKIDVDNLGFIFSYG
                     IDNLSLSRYVTLSRMLHYFFNKIVFNELSNTNNLKNTYTVLSGGDDLFLISPLRNTIE
                     LIKTLQTKFNDFTCRNNDISFSTGVFICHDNYPMGKASELAEECLEKAKSEGKNKIHL
                     FNVSVRYDKCYEMQSTIEWFASKLIDNKSTITQSFIYRLLEYVRMAMSNSFSRFLYLP
                     RFKYDIARNIVQKDKEGNIINGDDIQKLEGLFHKSNSDRFKLLEVLLMLTIYSLRKHK
                     PSKEENA"
     gene            complement(5099..6055)
                     /gene="cas6"
                     /locus_tag="PLX22_06515"
     CDS             complement(5099..6055)
                     /gene="cas6"
                     /locus_tag="PLX22_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOT19588.1"
                     /translation="MELLNTEKKIVPLYKFEINIKAITPIEFYNFPGIVLRGAFGWML
                     KQQTCLEKNLEKCRPCQFVQYCPYASIFESPNTGDASIMAKATNFPHPFVLSPVVRWG
                     TIIQPDEQFTVYLSIFGESIKYFHFFIKALIAMGNKGISKTKGTYEITSIANYSNNDL
                     IYTKDDGYIADTLQPMEVECIDTTTATVQFITPCFLKFNNEILKKPTIEAIITNIARR
                     YKIISNIYGGDAIVNNGDYFYDTAIHLHNVTISDMDIEWYSTNRFSKRQSRMMKVQGF
                     MGSMTIEGNIREVYPLLKIGEAIHIGKQTSFGCGLIRLQNVQ"
     gene            complement(6194..6370)
                     /locus_tag="PLX22_06520"
     CDS             complement(6194..6370)
                     /locus_tag="PLX22_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19589.1"
                     /translation="MSSIVSYGNLFNQEEIEKFLCDMFLNVNKGVLEKYSYNDYSFYD
                     LICGNRNKAVVKIK"
     repeat_region   7401..7657
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7401..7435
                     /rpt_unit_seq="gtctcaatcccctatcagcggggcagttttcagac"
     gene            complement(8071..8544)
                     /locus_tag="PLX22_06525"
     CDS             complement(8071..8544)
                     /locus_tag="PLX22_06525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOT19590.1"
                     /translation="MADIYLISDYGKLYKQNKVFHFLYPDGTVAKFFPHNTQRMYVIG
                     NIQITAEALKMISHNKIEVIFLNKNGFFNGKLTFGDSKNVLLRKKQYEKLNDNDFILK
                     WGPAGLKEFSEGTDAVASYGSCEDKDRMSSAARRQIYNPYSKQGTPPFEVRFGGW"
     gene            complement(8549..9790)
                     /locus_tag="PLX22_06530"
     CDS             complement(8549..9790)
                     /locus_tag="PLX22_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HOT19591.1"
                     /translation="MKKVMFITVGTGRERTDIAQAIVESIRIQSPDFVVFYCTHKSSN
                     ETMPEIYALNNALQVKSTVEIISDENNLEKIKKEISHSMAQYKKNYALNTVDYTSGTK
                     AMSAGIIVAAIENSCDYISYITGTRDNQGRVVTGTEQINTFRLNQIYATKYVELGKQF
                     FNNYLFGAAIDLFEKAKNICHDETLNNEVELYISLAQALNAWDKFDHEQAKNIFTAIT
                     KNKQAVVLLESKEIEKTIEIMKRHLHAIASSQFSDLLIIDLLKNAERRFAEGKYDDAM
                     ARLYRLLEFIAQVLLDKQNIYQKDAHGKSDTSKLDVEKLPQHLKEKYTPAIGLSKSYE
                     LLSDLNDETGKKIYDIMQKKDVKQILGIRNGSILAHGFVPVCRDITQKCIEIIKEIAS
                     IYFTLQQFEKLYHELEFITLE"
     gene            complement(9696..>11484)
                     /locus_tag="PLX22_06535"
     CDS             complement(9696..>11484)
                     /locus_tag="PLX22_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19592.1"
                     /translation="FVTITMKYPALNFILHRDAGEDKLIEAIDGLKYTFKKYRDEIIG
                     IIEFLASPVCATSKRCSIYELWTRYEEDCVNHGKKSVNTYTFRYNIGLLQILGILDSS
                     NNNDFEPSYTVKVYDINKSPLEHINTAWEEIETIYNTKKASMKLLVQQYILDGLSTKD
                     GKDGKDGKDDKDVFDKRRNFFEEFFLKDSYDKMLLIQNKDFGEQACEYNNCFLSIAGT
                     TANTFKKQDGKYTIEDVIAYIASEVKKLQKQLIQQYVVSIKKPDDVPIITLKSVMYTE
                     FMLLLLESIRIPVHKLSDCVTITKTSEHRDAINNYEAFDRQQHKAYTVASFILYMLVI
                     SSYFTGFETFDNKDYIEYMQKNNGTRHLDGGVTMDSDKFCKEVVEEAAGYVKKELKNT
                     GYKNITYQEIAHYAEQFFTTRYVLFNNYKKVPTSPSTNYSYYSNEALSKTIDNYIFKQ
                     QCIFRVFDVYNRFQNQNAVEKHLASFEIERFLGNYLANAIQYYVNNNNNIYPQNILPM
                     INHFAIVVVHDIENKWVNIIKLFNPHAVVIKINDAIFNGDSKDDNAVYQYKKCEVLYE
                     ESYVYNCGDRQRTYRYSTGNSGKYTNPKP"
BASE COUNT         3511 a         2213 c         1656 g         4104 t
ORIGIN      
        1 ttttggggca ttatactctg atgccccggg agatgccact cattttcaac agattttggg
       61 acaagctcga tctaaattta ctacatgttt attttcagga ctgccatata ttcttgcttc
      121 aaaattcaat gctttcttta ttacaacttt gtcttttcta acatcatcgc gttcaacctt
      181 tggttctaat ttaaattttt ttcgcaagga ttctaacatt gcagtacgta acatgccttt
      241 tactgaagat cctggtataa aaggtttatt ggttaattta tctgtaaaaa ttttattaat
      301 ttgtaaattt ggattatcac ttatttgttt tgtaaatcct tctaatgctt tcgctttacg
      361 aataatacta tctggttctt cataaacctt attatgcaac atacttttta atttagtcat
      421 caatacttta atatcatttt cattattctt ttttacaatt tcatcaagca aggtattgaa
      481 attttctttt tcgttttctc gcatcagcat aaatgcacgg actacatcaa taataaatac
      541 ttcatcattt tttggattag gcattatatc ccataaatca taaaaggttc cattatgaat
      601 aattaatgga gtaataatat ttatagttgc tctgtattgt tccattgact ccacaccctg
      661 ttatccaaaa tatagcggga aagcatatcc atattgaaca atagactttt cctgatgtac
      721 attgtttacc atactaccca acccattacc cgaaaatacc gaaccttcat gataaaacac
      781 aactggcttt ttacaataca ccaggctacc attgatacca cattgactat ataagcctcc
      841 aagcttacca tagcgggtaa aggtggtata acatcgagga tataaatccg ctcctataca
      901 tacacttaag gtaacataat tatccgaaaa ataatcaaac attgctattt cctgctgagt
      961 tattttttct aatttcattt taaaaactcc cttccctata ctgcaatcat tacccagccc
     1021 gtttaatcca acagcttcaa caattgacgc aaaccatcct tcagaatatt gttcatcatt
     1081 tacttttata taaaaaacca aaggcatttc tgaatataaa aaaacctcac taaaaagtgc
     1141 tccttcacga gcgtgtagtg tgatacgatc aatagcattt cttgtaattt caaggcgccc
     1201 aatctttgta ctatcaaata tattattaaa taactcatca cttactatag cttgtgataa
     1261 atttttctgg tttttagaaa taaaatcaat agttacaaac tgtatttttt tacgcttttt
     1321 ttctttttcc agtaaaccct ttgcttcaga tttattgtta ctttcaattc gtggtggatg
     1381 cagtaatggc ttaaacatat aaccatacgg catcatagat gataatagaa aaggcgggct
     1441 gcttttaaat ttttctaaca agccttccag tgcatcagcc ccttcaaaat atcgtatgga
     1501 ccagcaaaag gctccaaaaa tggtatcagc tttgtacatc atagaataaa atgacatagg
     1561 ctggcaaact actttatata cacttgccat gttttactcc aatttaaatg gttgtccatt
     1621 tatgctaaca ttttcaaacc gtattttacc atagccacgg ctaacactac cacccaatgc
     1681 atccttttga agcatcagta aacctttttt taataaatca atagctacat tattatcatt
     1741 gtcaaaaaca cgtaaggcta tttcaaaatc aaatgtaagt ccaggtatta cccgttccat
     1801 tgtgcgtggc gattctgcat gaccacccaa tctattaatc atattctctg ttttttcttc
     1861 agttgcctgt aaatttttat catataattc tttctttgat tcctcagata aaaatgcatc
     1921 cctgaataaa accctggtta tttcattgct ttcttttgca gttgttccaa atactttaca
     1981 aacaggacat ttaccgcatg aacagggttt acttttatca tttgaactaa taccatgctc
     2041 caattcaagt aatgagcgta ttttgccttt caaagaagaa ccgggtatat agggaaaacc
     2101 tgtacgaacg tctttaagaa ctggattatc caattcgcca atacccactc cttttgcatt
     2161 ggctccaata tgtaaaccag tttccactaa tattttgccc tttactattt taatctcttt
     2221 tagctgtgcc atttagcccc tcctttgttg atgttgatgt tgatgttgct gttgctgttg
     2281 cgattgggaa aattcattca aaaaagtaaa ataacaaatt attgcctgat aatgaagcaa
     2341 aaaatttcta agactattga aatcattaat cttatcaatt tgtgctgaca tgtgatcttt
     2401 aaactcaaca ggtactaatc ttttattatt tacttttctt ccagcgccat aggcaatctt
     2461 tgctttcata aattttacca tgggtaaaat ctgtttttga aaatcatcat ttgatttacc
     2521 atacccacgt ttttccagca ctttaagctc atcataatat ttacgcagct gcgatgtctc
     2581 aagacctctg tctttaaaaa ttttagcttt tgcctcgggt tcagtagtta aatatttctc
     2641 atcaatctct atcatgcatt ttcctccttt gaaggtttat gttttcgtaa tgaatatatt
     2701 gttagcataa gcaatacttc cagcaattta aacctatcag aatttgattt atgaaaaaga
     2761 ccttctaatt tttgtatatc atcaccatta ataatatttc cctctttatc cttttgtact
     2821 atattccgcg ctatatcgta tttaaacctt ggtaaatata aaaaacgtga aaatgaattg
     2881 ctcatagcca ttctaacata ttcaagtaac ctgtaaataa atgactgagt tatagtagat
     2941 ttgttatcaa ttaacttaga agcaaaccat tcaatagtag attgcatctc atagcactta
     3001 tcataccgta ctgaaacatt aaacaaatga attttatttt taccttcgga ttttgctttt
     3061 tcaaggcatt cttcagctaa ctcggatgcc ttacccatgg gataattgtc atggcaaata
     3121 aatacgcccg tagaaaaact tatatcattg ttcctgcacg taaaatcgtt aaattttgtt
     3181 tgtaaggttt ttattaactc aatggtattt ctcaaagggc ttattaaaaa taaatcatcc
     3241 ccacctgata atacagtata ggtatttttt aaattatttg tgttcgatag ttcattgaaa
     3301 actatcttat tgaagaaata atgcaacatt cgtgataatg taacatatcg tgataagctc
     3361 aaattatcta tgccatatga aaaaataaag ccaagattat caacatcaat ctttataaac
     3421 gatagcaatg gaacgccacg catggtattt tcttcatctt cattataatg gataactgat
     3481 gataaggcaa tttcttcaaa cgtttttatt tcattattta caatgggcac ataattattc
     3541 aactctaaca gtggcagtgt ttctactgac tcatcaagcg tgtatatggc gtatgcatta
     3601 cgatatggaa ggatatcgtt tgcaaaacta atggaaaagg ttccattaaa aaatgatgaa
     3661 tcggcattat gagcattatc atgaaatacc agatacaatg gtttttttac aatttttccg
     3721 cccaatgtaa acattgtatt acaaaaatca caacgttgac ccaattcatc tgaatagtta
     3781 atatttttgc tatacttacc acatgcaggg cacatttctt cttcagagta attaatatca
     3841 attacacaat tgccaccgct aagaatattt ttaaatttag agtatttagc tattgaaagg
     3901 ttataattta tttgtccaat caacgatgaa aatttatcat gcccaaaatc attaattgaa
     3961 gcttttactg aattatcagt tattattgca aaatcaccat gaaattcttt aaacagccat
     4021 tgatcaattt catgctgtac cttatccaga attgataaaa ctttttctga atttggtaaa
     4081 agcattacaa atttgccact tgaggacatt agctgagtaa atggtgcaat accggattct
     4141 ttatatagct tatactgata tgccagacca ataacagaaa tatagaatga tcttccacgt
     4201 attatcttgg aacttccttt aaatgactgc tgatggtgct ggaatataaa ctgttgtata
     4261 cctgatatat cagcagcaaa taatacaatt gccttttcat cctttgatgg caattgctca
     4321 gaattataat tatacaaagt taaagctatt gcagcagtac ttacagcatg atcatataat
     4381 gagctatcgc acataatatc atttgttgcg gccggaatac accatgtata tttttttaac
     4441 agatagttga tacaatgggc aaattgaata atattattac tattgcccca ttgcaaacaa
     4501 atttgatgaa atgaatctat aaattcatcc cacagttttt tatattgcga ttctaatgta
     4561 tcatcctcct gttgcaatgc aacggggtaa acactattga cattaagcct tgctatttta
     4621 tatatagagt gattcaaatt tttatgctta tcccctattg agacctgcgg aaagattgaa
     4681 cgtaaatgtt tcttcaaatg tataccatgc ttaaaatgtt catcttcccc tgatctatcc
     4741 agtgatgatg aaactctatc tgctaattgt ataatttctt ctttatagtt tgaaggtttg
     4801 tgatgttttg aagccatatc acgaatttca ttccaatcaa gatcctttgg caattgaacc
     4861 gttttacagt cattttcaaa aaaatcaaaa gtccataatg catgtttgta accataccca
     4921 ctttcagttg aaggcaaaat cattgcatcc atagcggaag gaaaattgga atgcggttta
     4981 tcaccaaatg cacgttgttt aaattttcca atatcatgga acaaacttgc aaataaaata
     5041 tgtaactgat gcttaagatt ttcatccatt agtggtgact ccatccttct ttaatttttt
     5101 attgtacatt ttgaagtcta atcaagccac acccaaacga agtttgtttt cctatatgta
     5161 ttgcttcgcc aatttttaac agcggatata cttcccttat attgccttct attgtcattg
     5221 accccataaa tccttgaact ttcatcatac ggctttgcct tttagaaaac ctgttggtac
     5281 tgtaccattc aatatccata tcacttattg taacattatg caaatgtata gccgtatcat
     5341 aaaaataatc tccattattt acaattgcat caccaccata aatattggaa attattttat
     5401 aacgccttgc aatatttgtt ataattgctt caatagtagg ctttttcaat atttcattgt
     5461 taaattttaa gaaacatggg gtaataaact gcacggtagc tgttgtagta tcaatacatt
     5521 ctacttccat tggctgtaat gtatcggcaa tataaccatc atcttttgta tatatcaaat
     5581 cattgttact ataattggca attgaggtaa tttcatacgt gccttttgtt ttacttattc
     5641 ctttgttacc catggcaata agagctttta tgaaaaagtg aaaatatttt attgattcac
     5701 caaaaattga caggtaaacc gtaaattgct catcaggttg tataatggta ccccagcgta
     5761 caactggtga aagaacaaag ggatgtggaa agtttgttgc ttttgccata atgctggcat
     5821 cacctgtgtt gggggattca aatatagaag catagggaca atactgcaca aattgacatg
     5881 ggcggcattt ttccaaattt ttctcaaggc atgtttgttg ttttagcatc catccaaaag
     5941 caccacgaag tacaatacct ggaaaattat aaaactcaat tggcgtaatt gcctttatat
     6001 taatttcaaa tttgtaaagt ggaacaattt ttttttctgt atttaacaat tccatttcat
     6061 caattttgtt tttgcttaat agtgattaac cataatagtg taacaaatta aaaattcctt
     6121 tatataaaac gtcaagttgt ttttgttata caggcaatta tttttcaaca tttataaaaa
     6181 cagtataaat attttattta atttttacca ctgctttatt cctgttacca catatcaggt
     6241 catagaagct gtaatcgtta tatgaatatt tttccagaac acctttattt acatttaaaa
     6301 acatatcaca caaaaatttt tcaatttctt cctgattaaa cagattacca taactaacta
     6361 ttgaactcat tgtttacctc cttgtaatgg tttatataat agtaagacat ggatatagca
     6421 aaaaatattg gaattttttt tatttttatt catactttat tttgtggggc aacaaaagtt
     6481 ttatcctttc gtaaggggat atttatgcgc taaatacccc cttaccaacc cccaaagcgc
     6541 acctcaggcg gcggtgtgcc ttatcccgct gtatggatta tatccctgcc atcgtgctgc
     6601 ggaagacatc ctgtctttgt cctcgcacga gccgtagagc gctacggcat ccatgccttc
     6661 gctgtttttt cattaatccc gcagtgataa aaaatggggt cagagccgtt tttgttttca
     6721 caagagaaaa gtggcttcat acgcctgcat ccaaagacgc catacatccc tgtatggctg
     6781 tttattgttg cgatgacacc ccaggtatcc ccatttataa aaggtgggga tggtgtgaga
     6841 atgttcaata gcacaaaaac tcataaaatt tcttaatttt aaaaaaggga aaattttttc
     6901 tgatggtata gtgttcactt aacaataaat aagcacgaat ttctatctct ttttaaaaac
     6961 aaaaacattt tacatccagc acacaattca gtatgcccat aaaacaattc ctaacagctc
     7021 atatctacat taccagcaac caccaaatcc tcaccaacac tttcaacatt ataactttca
     7081 actttcaacg gttacgtctc aatccccttt cagcggggca gttttcagac cgggaacgta
     7141 gttgaaatca acaagaattg tgttaagtat aagtctcaat cccctatcag cggggcagtt
     7201 ttcagactta attatatcga aggtcggtat gactatacta ttcgacagtc acaatcccct
     7261 atcagcgggt aacatcaaca ctgtaaactc ttttatcttt tataacttca ccataatcac
     7321 agctaatagc tatatcacca gcaaagaatc aatccacacc acaactttca acattataac
     7381 tttcaacttt caacggttac gtctcaatcc cctatcagcg gggcagtttt cagactgact
     7441 gatgaagcta ttaccttagc agcccagaaa cgggcgtctc aatcccctat cagcggggca
     7501 gttttcagac atcactctgg tgaaaaacgt taacaaacga ttcttttggg tctcaatccc
     7561 ctatcagcgg ggcagttttc agacgagcat gggtacaatg tcgcagttgt gcataatttc
     7621 gagtctcaat cccctatcag cggggcagtt ttcagaccct taccctgtaa acctctggaa
     7681 tttccagcgt ttttaagccc attttcgcca acctatcgca aaaatcacta tttttaccat
     7741 gattttgtat tacaaacttg taactgctgg attttccagc acttccacgt gcgccaacct
     7801 ccccccaaaa acgccacttt ttacactttt agcaggttgg cgatatacaa agttgtatat
     7861 catggtactg ttttcaaaga atatcttaca tcatctgtta ctgcctatag catgaagcat
     7921 ttcctgtcaa ctattttttt acttctgtga aaaattttac cgtaactatc gcccataaaa
     7981 attacaatgg ggtcagagca tgtataactt ttttggggcg acaaaagttt tatcctttcg
     8041 taaggggata tttatgcgct aaatgccccc ttaccaaccc ccaaagcgca cctcaaacgg
     8101 cggtgtgcct tgtttactgt atggattata tatttgcctt cgtgctgcgg aagacatcct
     8161 gtctttgtcc tcgcacgagc cgtatgacgc tacggcatcc gtgccttcgc tgaattcctt
     8221 taatcccgca ggaccccatt ttaaaataaa atcattgtca ttgagctttt catactgttt
     8281 tttacgtagc aatacatttt tactgtcacc aaaggtaagt ttgccattaa aaaaaccatt
     8341 tttatttaaa aatataacct ctattttatt atggcttatc atctttaagg cttcagcagt
     8401 aatttgaata ttgccaataa catacatacg ctgtgtatta tgcggaaaaa attttgctac
     8461 agtaccgtcg ggatataaaa agtgaaacac cttgttttgt ttatacaatt tcccgtaatc
     8521 agatatcaaa taaatatctg ccatagtatt actccaatgt aataaactcc aattcatgat
     8581 ataatttttc aaactgctgt agtgtaaaat aaatgctggc tatttcttta ataatttcta
     8641 tacatttctg tgttatatct ctgcatactg gcacaaaacc atgtgccaag attgagccgt
     8701 ttcgtatgcc cagtatttgt tttacatcct ttttttgcat tatatcataa atctttttac
     8761 ctgtttcatc atttaaatca cttaacagct cataggattt acttaatcca attgctggtg
     8821 tatatttttc ttttaaatgt tgcggcagtt tttcaacatc aagtttgctg gtatcgcttt
     8881 taccatgagc atctttttga tatatatttt gtttatccag caacacctgt gcaataaatt
     8941 caagcaagcg gtacagccgt gccatagcat cgtcgtattt tccttcggca aagcgcctct
     9001 cagcattttt caataaatct ataatcaata aatcagaaaa ttgactactt gctatagcgt
     9061 gcaggtgtcg tttcattatt tctattgttt tttcaatttc cttactttca agtaatacca
     9121 ctgcctgttt attttttgtt attgcggtaa aaatattttt tgcctgttca tgatcaaatt
     9181 tatcccatgc attcaaagcc tgtgcaaggg atatataaag ttctacttca ttgttaaggg
     9241 tttcatcatg acatatattt tttgcttttt caaataaatc aatagctgct ccaaacagat
     9301 agttattaaa aaattgtttt ccaagctcaa catactttgt tgcataaatt tgattgagtc
     9361 taaatgtatt tatttgttca gtacctgtca ctactcgtcc ctgattatcc ctggtgccag
     9421 ttatatagga tatataatca cagctatttt ctattgcagc tacaataata cccgccgaca
     9481 ttgccttggt tcccgacgtg taatcaacgg tatttaatgc ataattcttt ttatactgtg
     9541 ccatgctatg gctaatctct ttttttattt tttcaagatt attttcatca cttataattt
     9601 caactgtgct ttttacctgc aaggcattgt ttaatgcata tatttctggc atggtttcat
     9661 tgctactttt gtgagtacaa taaaacacaa caaagtcagg gctttggatt cgtatacttt
     9721 ccactattgc ctgtgctata tctgtacgtt ctctgcctgt ccccacagtt ataaacataa
     9781 ctttcttcat acaacacctc gcatttttta tattgatata ctgcattatc atctttacta
     9841 tcgccattaa aaattgcatc gtttatcttt atcacaactg catggggatt aaacaatttt
     9901 ataatattaa cccatttatt ttcaatgtca tggactacta caatggcaaa atggttaatc
     9961 attggcagta tgttttgggg gtatatgtta ttattgttgt tgacgtagta ctgtatggca
    10021 ttggcaagat agttacccaa aaacctttct atttcaaatg aagcaaggtg cttttctaca
    10081 gcattttgat tctggaacct attatacaca tcaaaaaccc tgaagataca ctgctgctta
    10141 aaaatataat tatcgatagt ttttgacaat gcttcattgc tgtagtatga ataatttgtt
    10201 gagggcgatg ttggtacttt cttatagttg ttgaataaca cataccgtgt agtaaaaaat
    10261 tgttcagcat aatgagctat ctcttgatag gtgatattct tatagcctgt attctttaat
    10321 tcttttttta catatccggc agcttcttca acaacttctt tgcaaaattt atcactatcc
    10381 atagtaacac cgccatctaa atgccgtgtg ccattatttt tttgcatgta ttctatataa
    10441 tctttattgt caaaggtttc aaaccctgta aaataagagc taataaccag catatagagt
    10501 ataaaacttg caactgtata ggctttatgc tgttgcctgt caaacgcttc ataattatta
    10561 attgcatcgc gatgctcgct tgtttttgtt atggtaacac agtctgatag tttatgtacc
    10621 ggtatcctga tggattctaa cagcaaaagc ataaactcag tatacatgac acttttaaga
    10681 gtaataatcg gtacatcatc tggcttttta atgctgacaa catactgttg tattagttgt
    10741 ttttgcaatt tttttacttc tgatgcaata taggcaatga catcttcaat agtgtattta
    10801 ccatcttgtt ttttaaaggt gttggcagta gtaccagcaa ttgataaaaa acagttattg
    10861 tattcacacg cttgctcacc aaaatccttg ttttgtatta gcaacatttt atcataacta
    10921 tctttcagga aaaattcttc aaaaaaatta cgccgtttat caaaaacatc tttatcatct
    10981 ttaccatctt taccatcttt accatcttta gtagatagcc catccaatat atattgttgc
    11041 accagcagtt tcattgatgc ctttttggtg ttgtagatag tttcaatctc ttcccacgct
    11101 gtattgatat gctctaaagg cgatttattg atgtcatata cttttaccgt atagcttggt
    11161 tcaaaatcgt tattatttga tgagtccaga atcccaagta tttgaagcaa accaatatta
    11221 taacgaaatg tatatgtgtt gacacttttt ttgccatggt taacacaatc ttcttcgtat
    11281 ctggtccata actcgtatat tgaacaacgc tttgatgtcg cgcatactgg gctggctaaa
    11341 aattctatta tccctattat ttcatccctg tattttttaa aggtgtattt caacccatct
    11401 atagcctcaa tgagtttatc ttcacctgca tctctgtgaa ggataaaatt taatgcaggg
    11461 tacttcattg ttatggttac aaat
//
LOCUS       DAOSOX010000070        11440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5645_length_11440_cov_10.205270, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000070 DAOSOX010000000
VERSION     DAOSOX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5645_length_11440_cov_10.205270"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1080
                     /gene="fliN"
                     /locus_tag="PLX22_06540"
     CDS             28..1080
                     /gene="fliN"
                     /locus_tag="PLX22_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOT19593.1"
                     /translation="MGDGSLSQDEIDALLQGADDMLSGSARVPAGDFAPVQAALSPVE
                     REAVADFLNSSMSSVMPALSGYLGKNIIIGNALVEVKNADQIRADFPKQYVQVSVNFS
                     GGVNGRNIVLLSMNDAGVISSLMMGDDSGIPPATLSEAHQSTLQEFVSQMMSTIATQL
                     SNKIGKGVNTTAPDLAIVQGDIAVPGTPDVVKITYNLTIQNLINSKMYHLMNIELAGE
                     IARGSYVPQMQQQEYRTEAAPQKSAQVGISPVRFPPLGEGMQSVPTGNINLLLDVPMT
                     LTVELGRTRQLVKDILGLGEGSIIELDKLAGEPVDLLVNGKLIARGEVVVIDENFGVR
                     VTDIVSPAERFAKMQT"
     gene            1115..1906
                     /locus_tag="PLX22_06545"
     CDS             1115..1906
                     /locus_tag="PLX22_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOT19594.1"
                     /translation="MIVAATIKKRLCVSSMKFLYGVILLCLIFILCPITSNYRPNENF
                     LYAKNTKTAKLDKIESKNTITEKKEEPIGVETKSDDEQKENEASKNDYLYNYERPQIE
                     EESYVWLIFKTIFVIGILIGGFYFFFKFITEKVGIAAVGQEVITILSVVPLGQNKFLQ
                     VVDIAGKVYVLGVSDNNINLITEIKDKDDVDRIRLLSSKSTPVKPGGFQDYLSTQLGK
                     VLDRIKNPKEGISQHYSYNQEHTGNDNVLLTYKQRLKKLNGDDSV"
     gene            1970..2707
                     /gene="fliP"
                     /locus_tag="PLX22_06550"
     CDS             1970..2707
                     /gene="fliP"
                     /locus_tag="PLX22_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495865.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOT19595.1"
                     /translation="MGIGMIVSDAAFAQKAGNVRMPIPKIGFDIEEAKTPKDVSLSLQ
                     ILFLLTILTLAPSIIMMMTSFTRVVIVLDFVRRALSLQQMPPTQVIVGLAIFLTIFIM
                     SPTLTEINDKALQPYLKGQISNEEFYNKGMQPLREFMLKQTRKKDIALFIELAKLERP
                     KTQDDVPTYCLIPAFMISELKRAFEMGVYIFIPFIVIDMIVASALMAMGMIMLPPIMI
                     SLPFKIVLFVLVDGWNLLTYELVRSFM"
     gene            2717..2989
                     /gene="fliQ"
                     /locus_tag="PLX22_06555"
     CDS             2717..2989
                     /gene="fliQ"
                     /locus_tag="PLX22_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOT19596.1"
                     /translation="MTDIEVIKLLREALMVTMIVSAPILGIGMFVGLIISIFQTTTSI
                     QEQTLTFVPKIIAIFAAFILFAAWMLHTLMSYTKELFMMISKLGGG"
     gene            2989..3768
                     /gene="fliR"
                     /locus_tag="PLX22_06560"
     CDS             2989..3768
                     /gene="fliR"
                     /locus_tag="PLX22_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOT19597.1"
                     /translation="MEYFVYQFQSFFLVLVRMTAMMMIAPFFSSGVIPLLMRGALSFF
                     VSLVIFPLLSISAGAVTGNMGVYYLMVVQEIIIGLYIGFLVSVVFSAFQLSAQYFAVQ
                     IGFGINEVIDPLAQVSIPLIGQLKNFIGLLVFLTINGHHFMIKAIYNSYELAPAFGLK
                     GDSVQVFLKFLVHSFSGMFVIALKISLPVVAVVFLMSVSMGILAKAAPQMNIMMLGFP
                     FKIAVAFGMMILISPLIVRVMNVSLERTFQFIVKVIRYWPV"
     gene            3765..4925
                     /gene="flhB"
                     /locus_tag="PLX22_06565"
     CDS             3765..4925
                     /gene="flhB"
                     /locus_tag="PLX22_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOT19598.1"
                     /translation="MKWYAITDKDAEIVIFESRAIVFDMQLFAAEDEGRTEEPTEKKL
                     REAREKGQVAKTEELPQALVIIFAMITIVVFCGFIFETIIRMMHYYLTNFSHFNLTER
                     SLFHEAIASGVESFKILIPIFVATVIAALIGNIAQVGFQISTHPLKFDLSKIKFDPAT
                     MARKIFFSRQIAMNLFKSIFKVVVIGFICYLVIINDFEELIKTPDISVGLAFQNVSII
                     ALKIIIWAAVCILVLSIPDYVFQKREYIESLKMTKQELKEEWKETVGDPHVRARLQEM
                     QRELIMRNMIREVPKADVVVTNPTHFAIAMRYEPEAMYAPMVIAKGVDTMALTIRQIA
                     LDNSILIIENRPLAQELYKRVDVGDIIPEDLFYAVSLIYAEVYKKRNYKVAM"
     gene            4940..7021
                     /gene="flhA"
                     /locus_tag="PLX22_06570"
     CDS             4940..7021
                     /gene="flhA"
                     /locus_tag="PLX22_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOT19599.1"
                     /translation="MAERIYDFSWMQKSDMLLGIGVIAVVTMLVIPLPTFLLDLLLAI
                     SLMLGILILLVVMYVPRSFDFSVFPALLLITTVYRLALNVSSTRLILLQGAAFDGKII
                     RTFGDFVVGGNYVIGFIVFIILTAVQFIVITKGATRTAEVAARFTLDAMPAKLMSIDS
                     DLSNGIINEEEALHRRREVRKEADFYGAMDGASKFVQGDVKVGILITIINIIGGFIIG
                     MVMRGESFNVAIHTYTLLSIGDGLVAQIPSLMVTTATGVIVTRAVSEESLGKDLSTQL
                     GSQPRALMITAAALGLSAIIPGFPKITLILLAAGLGALGYLMKQTEEEKMQKTKIEQK
                     EAALKTHKPESVIPLIQVDALEVEIGYSLIPLADPDQGGTLLDRITNIRRRSALEMGL
                     IVPPIRIRDNMELPPQDYSILIKGVEVGHGSLQVGKLMAMDPGTVEEKIEGVEFTEPV
                     FNLRAIWIDPDNRDVAERRGYTVVDCPTIIATHLTEIIRRHADEILGRQEVQQLIDTI
                     KNDYPAVVNELIGEKKLSIGEIQKVLQNLLRERVSIRNMVTILETLASYSDYTKDIGL
                     LTEYVRVALARQLCREYADKNNTLSVITVDPEIETIIRSSIHEDPVEGRIISLDPDTH
                     REVLDSLFDSYSRARSAGYAPVFLVSPHIRSVLFTLLEREVPDPVVLSYNEIVPEIRV
                     NVIGTALLRAA"
     gene            7055..>7706
                     /locus_tag="PLX22_06575"
     CDS             7055..>7706
                     /locus_tag="PLX22_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19600.1"
                     /translation="MRYVKIKARTYNEAMMKLKMEHGDDAIPISHKNIKEGGIFNTGL
                     FARDFVELTAAIPDVKPSKLKPRKNIDLTVGGNENINDLLKQVIDKPESQPQQKPNTG
                     LEQLIVQGMEKPNIKTTESPHPVKSPVDEVMVERTDQPYIKLEKEFQELKMLLKNMVA
                     SGQKKPIDDIVIDSNDIYFKPFKEILRKNDIDDDECNRLLEDVKRNVSSEDMKDRYK"
     assembly_gap    7707..7806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7807..8523
                     /locus_tag="PLX22_06580"
     CDS             <7807..8523
                     /locus_tag="PLX22_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOT19601.1"
                     /translation="LEDVKRNVSSEDMKDRYKIEKTLRELIKSKIIVSGPIDKGKRKK
                     VLMFVGPTGVGKTTTMAKLGAILSLRQGLKVSFVTIDNYRIAATEQLKKYAEIMRIPI
                     HVVTDSHEFKEIVENEKAEIIMVDTSGRSHRNDLKIAEIKNFADACNCEIHKILCVSA
                     NTKRSDLNEVFKAYDILHIDSIIVTKVDETSYIGNVISIADKYNKPISYFTNGQEVPN
                     DIAVADPEIMVNMILESTMR"
     gene            8535..9413
                     /locus_tag="PLX22_06585"
     CDS             8535..9413
                     /locus_tag="PLX22_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOT19602.1"
                     /translation="MDQAANLRRLVVENNTARRTKTIAITSGKGGVGKTSIAVSLAIA
                     LAKRNYSITLLDADLGLANVNVVLGIIPKYNLYHVIKGKKKLEEIIIDVPEGIKIIAG
                     ASGFHQLANLDVKQREYFIKSLAELNADDYMIIDTGAGISQNVLSFLVASDEVIVITT
                     PEPTSITDAYGTIKAIAANDPDKTVKLLVNRAQTVTEAKKVAQRVINIAGQFLNIKVD
                     NLGFIFDDLYVAKSIRNQKPFIVSYPKSKASTCVEIIADRIGNIESDIDKGTGMLSFF
                     RRFFGHYEMDNDDGVV"
     gene            9533..10087
                     /locus_tag="PLX22_06590"
     CDS             9533..10087
                     /locus_tag="PLX22_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19603.1"
                     /translation="MLNIKKIAEISPEAKVSLIFGFLAFIVSLFFGLAVGNRIVILLT
                     RVLFFTIVYATIGYASMLVIKHFVPEVYEILNKVLNIGQSLDMATESSDVTMQSEYKN
                     SDDSDKGEGIVSSDSGNESIDVGATYNEAFTPLEAKDFTTFTTEESKPKKMGKHIVLD
                     DKKIHYEPKIMAEAIRTMIKRDKD"
     gene            10106..10906
                     /gene="whiG"
                     /locus_tag="PLX22_06595"
     CDS             10106..10906
                     /gene="whiG"
                     /locus_tag="PLX22_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HOT19604.1"
                     /translation="MANKKFQGFDATDEDELWKRYAKTQNQDIRDYFVIKYAPLVKYV
                     AGKIAMGMPSSIEFDDLVSYGVFGLIDAISKFDPDRGIKFKTYAMTRIRGAIFDELRS
                     IDWIPRSIRQKAKQIEQAIAELENKLGRTVEDEEIAKELGVTIEEFQSLLNKLSGTAM
                     LSLNDIWYVGDENDELSIMETLEAPDTLNPDVMIEKEEIRDYIVEAIKKLPDKEKKVI
                     VLYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLGRIKSNIVG"
     gene            10959..>11440
                     /locus_tag="PLX22_06600"
     CDS             10959..>11440
                     /locus_tag="PLX22_06600"
                     /inference="COORDINATES: protein motif:HMM:NF026154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HOT19605.1"
                     /translation="MKNLKELLKTLKDDDIAENEVEVYADSVKQALELASRDLGVDIS
                     ELDYQILEKGTSGLLGIGRQPYKVLIRPIKVEEEEDLTAIEQKLHAVPEADLKVQDVA
                     RNVDGSAKIRITKTGIWLTVTPPKGGGRRVDLSDVSNRIYSLRIQNADMAKIEKEIKH
                     P"
BASE COUNT         3665 a         1654 c         2474 g         3547 t
ORIGIN      
        1 tacgaatcaa acacattgga ggaaacaatg ggtgacggtt ctttgtcgca agatgaaata
       61 gatgccctgt tacagggtgc tgatgatatg ctctccggtt cagcaagggt accagcaggt
      121 gattttgctc ctgtacaggc agcgctttct ccggttgaac gtgaggcggt tgcagatttt
      181 ttaaacagca gcatgagcag tgtaatgcct gcactgtcag gctatctggg taaaaatata
      241 ataattggca atgcgctggt tgaagtaaaa aatgccgatc agattcgtgc tgattttccc
      301 aaacaatacg ttcaggtttc agtaaatttt tcgggtgggg taaatggccg aaatatagta
      361 ttgttatcaa tgaacgatgc aggtgtgatt tcatcactca tgatgggaga tgatagcggt
      421 attcctccgg ctacattaag tgaagctcat caaagcacgt tgcaggaatt tgttagccag
      481 atgatgtcca ctattgctac acagttgagc aataagattg gtaaaggtgt taacaccaca
      541 gctcccgatt tagcgatagt tcagggcgat atagctgtac cgggaactcc tgatgttgtg
      601 aaaataacat ataacctgac gattcaaaat cttataaact caaagatgta tcatttaatg
      661 aatattgaac ttgctggcga gattgcccgt ggttcgtatg ttcctcagat gcaacagcag
      721 gagtaccgaa ctgaagctgc accgcagaag agtgctcaag tagggattag ccccgtgcga
      781 ttccctccat taggtgaagg tatgcagtcg gtacctacag ggaatattaa tttgttgtta
      841 gatgttccga tgacgctcac tgttgagtta ggtcgtactc gtcagcttgt taaggatata
      901 ttggggttgg gcgaaggttc cattattgaa ctggataaac tggcaggtga accagtagat
      961 ttacttgtta atggtaagct cattgcccgt ggtgaggtgg ttgttattga tgaaaatttt
     1021 ggtgttcgtg taactgatat tgtaagcccg gcagaacgat ttgctaagat gcaaacataa
     1081 ttatcattaa aaagttttaa aggaaaaaaa attaatgatt gttgctgcaa ctataaaaaa
     1141 acgtttatgt gtatcgtcaa tgaaattttt atatggtgtt attttattat gtctcatttt
     1201 tatattatgt cccattacca gtaactatcg acccaatgaa aattttttat atgctaagaa
     1261 tactaaaaca gcaaaattag ataagattga aagtaaaaat acgataactg aaaaaaaaga
     1321 agaaccaatt ggtgttgaaa caaaatcaga cgatgaacaa aaagaaaatg aggcttcaaa
     1381 aaatgattat ctgtataatt acgaacgacc gcagatagag gaagaatcct atgtatggct
     1441 aatctttaaa acaatatttg tgatagggat actcatagga ggattttatt tcttttttaa
     1501 atttattact gaaaaggttg ggattgctgc tgttggtcag gaggttatta ctattttgtc
     1561 agttgtaccg ttaggtcaaa ataagttttt gcaggtggtg gatatagcag ggaaggtgta
     1621 tgtactgggt gtatctgata ataatataaa tcttataact gagataaaag ataaagatga
     1681 cgtcgataga atacgcttgc tcagttcaaa gtcaactcca gtaaaaccag gtggatttca
     1741 ggattatctt tcaacacaat tagggaaggt ccttgacagg ataaaaaatc caaaagaggg
     1801 gatatcgcag cattattctt ataatcagga gcatacaggc aatgataatg ttttgctgac
     1861 atataaacag cgattaaaaa aacttaatgg ggacgatagt gtgtagtatt atatgaaaag
     1921 ttgtaaatac aaaaggtttt ttataataat tttactgatt gttgtggtta tgggtattgg
     1981 catgatagta tccgatgctg catttgccca gaaagcgggt aatgtccgta tgcctattcc
     2041 taagattgga tttgatattg aagaggctaa aacaccaaag gatgtttcgc taagtttaca
     2101 gatactgttt ctgcttacta ttttgacctt agctccgtct atcataatga tgatgacatc
     2161 ctttaccaga gttgttattg tccttgattt tgtacgacgt gctctttctt tgcagcaaat
     2221 gccgcctact caggtcattg ttggacttgc tatttttctg actattttta ttatgtcgcc
     2281 taccttaaca gagattaatg ataaagctct gcagccatat ttgaaagggc agatatctaa
     2341 tgaagagttt tacaataaag gtatgcagcc attacgtgag tttatgttga agcaaacgcg
     2401 gaagaaggat atagccttat ttattgaact tgcaaaattg gaacgaccaa aaacacagga
     2461 tgatgttcct acgtattgtc ttattccagc ttttatgatc agtgaattaa aacgcgcctt
     2521 tgaaatgggt gtatatatat ttattccgtt tattgttatc gatatgattg tagcaagcgc
     2581 cttgatggcg atgggtatga ttatgttacc gccaattatg atttctctgc cgtttaagat
     2641 tgtgctgttt gtattggtag atgggtggaa tttgctgacc tatgaactgg ttcgatcatt
     2701 tatgtaggag aagaacatga cagatataga agtgataaaa ttgctgcgtg aagctctcat
     2761 ggttacgatg attgtgtcag caccaatcct tggaataggt atgtttgttg gactgataat
     2821 atcaatattt cagactacta catctattca ggaacagaca cttacgtttg taccaaaaat
     2881 tatcgcaatt tttgctgcgt ttatattatt tgctgcatgg atgttacata cattgatgtc
     2941 gtatacaaaa gaattgttca tgatgatctc caaactgggt ggaggataat ggaatatttt
     3001 gtttatcagt tccaaagttt ttttctggtg ctggtgcgta tgacagcaat gatgatgatc
     3061 gctccatttt tttcaagtgg agttattcct ttattaatgc gaggtgcgct ttccttcttt
     3121 gtatcgctgg taatatttcc gcttttatca attagtgctg gtgctgttac gggtaatatg
     3181 ggtgtatact atcttatggt agtgcaggag atcatcattg gcttatatat tggttttttg
     3241 gtaagcgtgg ttttttcggc ttttcagctt tcagcccaat attttgcagt gcagattggc
     3301 tttggtatca atgaagtcat tgatccattg gcacaggttt caataccgct tattggacag
     3361 ttgaaaaatt ttattggctt actggttttt ttaacaatca atgggcatca ttttatgata
     3421 aaagctatct ataattccta tgaattggct cctgcttttg gtttaaaggg agatagtgtt
     3481 caggtatttt taaaatttct ggttcattca ttcagtggta tgtttgtaat agcgttaaaa
     3541 atttcactgc ctgttgttgc agtggtgttt ttaatgtcag tgagtatggg aatactggca
     3601 aaggctgcac cacagatgaa tataatgatg ttaggatttc cctttaaaat tgcagttgcc
     3661 tttggcatga tgatccttat ttcgcctcta atagttcgtg ttatgaatgt ttcactggaa
     3721 agaacatttc aatttattgt taaggtaata cggtactggc cagtatgaaa tggtatgcta
     3781 ttacagataa agatgctgaa atcgtgattt ttgaaagcag ggctatagtt tttgatatgc
     3841 agctttttgc tgctgaggat gaaggtagaa ctgaggagcc aacagaaaaa aaattacggg
     3901 aagcacggga aaaaggtcag gttgcgaaaa ctgaggaact tcctcaggcg cttgttatta
     3961 tttttgcaat gataaccatt gttgtttttt gtggatttat ttttgagaca attatcagga
     4021 tgatgcatta ctatctcaca aacttttcac attttaatct tactgagcgg agcctttttc
     4081 atgaggctat tgcctccggt gttgaatcat ttaagatatt aattcccatt tttgtagcaa
     4141 cggtcattgc agcattaatt ggtaatattg cacaggtagg attccaaatt tcaactcatc
     4201 cattaaaatt tgatctttca aagataaaat ttgatcctgc taccatggca cgtaagatat
     4261 ttttttcacg acagattgcc atgaatcttt ttaaatcaat tttcaaggta gttgtaattg
     4321 gatttatatg ttatctggta atcatcaatg attttgaaga attaataaaa acaccggata
     4381 ttagcgttgg attggcattt cagaatgtat ctattattgc tttaaagatt attatatggg
     4441 cagcagtttg tatactggtg ctatcaattc ctgattatgt ttttcagaaa cgggaataca
     4501 ttgaatcgtt gaagatgact aaacaggaat taaaagaaga gtggaaagaa actgttggtg
     4561 atccccatgt acgagctcgc ttgcaggaga tgcagcgcga gcttattatg cgcaatatga
     4621 tccgggaggt tcccaaagct gatgttgtgg ttaccaaccc aacacacttt gctatagcta
     4681 tgcggtatga acctgaagca atgtatgcgc ctatggtaat tgcaaaaggg gttgatacta
     4741 tggcgctaac aatacgccag atagctctcg acaatagtat tttaattatt gagaacaggc
     4801 ccctggcgca ggaattgtat aaaagggttg atgtgggtga tataatccct gaagatttat
     4861 tttatgcagt ttccttaatt tatgctgaag tttataaaaa acgtaactat aaagttgcga
     4921 tgtaaagttg aggtagttta tggcagaaag aatatatgat ttctcctgga tgcaaaaaag
     4981 cgatatgctt ttaggaattg gcgttattgc ggttgtaacc atgctggtga ttcctctgcc
     5041 aacctttctt cttgatttac tcctggcaat cagccttatg ctgggaatac ttatactatt
     5101 ggttgttatg tatgttcctc gttcttttga tttttcagta tttccagctc tgctcctcat
     5161 aactacagtt taccgactgg cgcttaatgt ttcttcaacg cgattaatac ttttacaggg
     5221 agcagcattt gacggtaaga ttatccgtac atttggcgat tttgttgtag ggggcaacta
     5281 tgtcattgga tttattgtgt tcataatctt aacggcagtt cagtttattg ttatcaccaa
     5341 aggtgcaaca cgaacagcag aagtagcagc caggtttaca cttgatgcaa tgccagccaa
     5401 gttaatgagt attgattctg atttgtcaaa tggtataata aatgaagaag aggcattgca
     5461 tcgtagacgt gaggtaagga aagaggctga tttttatgga gctatggatg gtgcttcaaa
     5521 gtttgttcag ggtgatgtta aagtaggaat tttaataacc attataaata ttattggcgg
     5581 ctttattatt gggatggtaa tgcgtggtga aagttttaat gtagctattc atacttatac
     5641 tttgttatca ataggtgatg gtctggttgc tcagattcct tcactcatgg ttacaacagc
     5701 tacaggtgtc attgtaacac gggcagtttc tgaggaaagc ctgggcaaag atctatcaac
     5761 acaattaggt tcacagcccc gtgcattgat gataactgct gctgctctgg gattgtcagc
     5821 aattattccg ggatttccaa agattacact tattttgctt gctgcaggtt taggtgcttt
     5881 aggctatctc atgaaacaaa ctgaagaaga aaagatgcaa aaaaccaaaa ttgagcaaaa
     5941 ggaagcagca ttaaaaacac ataaaccaga atcagttatc ccattaattc aggttgatgc
     6001 gcttgaggtg gagattgggt atagcctgat accattagct gatcctgatc agggtgggac
     6061 actacttgat agaattacca atatacgcag gcgcagtgcc cttgagatgg gattgatagt
     6121 acctccaatt agaatacgtg ataacatgga attgccgccg caggattatt ccatattaat
     6181 caaaggtgta gaagttgggc atggtagttt acaggttggt aaactcatgg caatggatcc
     6241 tggcactgtt gaagagaaga tagaaggagt agaatttact gaacctgtat ttaatctcag
     6301 agcaatatgg attgatcctg ataacaggga tgtagctgaa cgtcgtggct atacagtggt
     6361 tgactgtccc actatcattg caacacactt aaccgaaata atacgaagac atgcagatga
     6421 aatattggga cggcaggaag ttcagcaact tattgataca attaaaaatg actatcctgc
     6481 agtggtaaat gagttaattg gtgaaaaaaa attgagtatt ggagaaatac aaaaggtttt
     6541 acaaaatctt ttacgtgaac gtgtttcaat acgtaatatg gttacaatac ttgaaacgct
     6601 ggcaagttat tctgactata ccaaagatat tggattgtta actgaatatg tcagggtcgc
     6661 tttagcacga cagttatgca gggaatatgc agataaaaac aacacgttat ctgttattac
     6721 agttgatcct gagattgaaa caattattcg ttcatcaatt cacgaagacc ctgtagaagg
     6781 aagaattata agtcttgatc ctgatactca cagggaggtt ctggatagtt tatttgattc
     6841 gtattccaga gcaaggagtg cagggtatgc acctgttttt ctggtgtcac cgcacatacg
     6901 cagtgtattg tttaccttgt tagagcgtga agttccggac cctgtggtgt tatcgtataa
     6961 tgagattgtt cctgaaataa gagtaaatgt aataggaaca gcattattac gggcagcata
     7021 aaattaatac atttataaag agaggtatac aacaatgaga tatgtaaaga taaaagccag
     7081 aacctataat gaagctatga tgaaattgaa aatggaacat ggggatgatg caattcccat
     7141 tagtcataaa aatataaaag aaggtggaat attcaacacg ggtttatttg cgcgtgattt
     7201 tgttgagtta actgctgcca tacctgatgt aaaaccctca aaattaaaac ccaggaaaaa
     7261 tattgattta acggttggtg gcaatgaaaa tattaatgat ttattaaagc aagttataga
     7321 taaaccagaa agtcagcctc aacaaaaacc aaacaccggg ttggagcagc taatagttca
     7381 gggcatggaa aaaccaaata taaaaactac tgaatcacca catcctgtta aatcaccggt
     7441 agatgaggtg atggtggaaa ggactgatca accatatatt aaattagaaa aagagttcca
     7501 ggagttaaaa atgttgttga aaaatatggt ggcatcagga cagaaaaaac ctatagatga
     7561 tattgttata gatagcaatg atatttattt taagccgttt aaagagattc tacgaaaaaa
     7621 tgatattgat gatgatgagt gcaatagatt gcttgaagat gtaaaacgta atgtaagctc
     7681 tgaagatatg aaggataggt ataaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnncttg aagatgtaaa acgtaatgta agctctgaag atatgaagga taggtataaa
     7861 attgagaaaa cactgcgtga attgattaaa agtaaaatca ttgtaagtgg acctatcgat
     7921 aaaggcaaac gtaaaaaggt tttaatgttt gttggaccaa caggggttgg gaaaactaca
     7981 accatggcta aattaggtgc tattttatct cttaggcaag gacttaaagt atcgtttgta
     8041 actatcgaca attatagaat agcagctaca gagcagctta aaaaatatgc tgaaataatg
     8101 cgtattccaa ttcatgtggt gactgattct catgaattta aagaaatagt tgaaaatgaa
     8161 aaagcagaga ttataatggt ggatacatca ggcaggagcc atagaaatga tttgaagata
     8221 gcagaaataa aaaattttgc tgatgcatgt aattgtgaga tacataagat tctttgtgtt
     8281 agtgcaaata ccaaacgcag tgatttgaat gaggtattta aagcatatga catcctgcat
     8341 attgatagta tcatagtaac caaggtggat gagacctcat atattgggaa tgttatctca
     8401 atagccgata aatataataa gccaatatcg tattttacta atggtcagga agtgccaaat
     8461 gatattgccg tcgcagatcc ggaaattatg gtaaatatga tactggaatc aactatgcgt
     8521 taaggaggtt aatcttggat caggctgcaa atttaaggcg attggtagtt gaaaataata
     8581 ctgcgcgcag aaccaaaact atagcgataa ccagcggcaa aggtggtgtt ggcaaaacca
     8641 gtatagctgt tagcctggca attgcattgg ctaaacgtaa ttattctatt acattgctgg
     8701 atgctgattt gggattggca aatgtaaatg ttgtattagg aattatccca aaatacaatc
     8761 tgtaccatgt cattaaaggg aaaaaaaagt tagaggaaat aattattgat gttcctgaag
     8821 gcattaaaat tattgctgga gcttcaggat ttcatcaatt agcaaatctt gatgtgaagc
     8881 aacgggaata ttttataaag tcgctggcag aactaaatgc tgatgattat atgatcattg
     8941 atactggtgc agggatttca caaaatgtat tgagtttttt agtagcttct gatgaagtga
     9001 tagtcattac tacaccggaa ccaacatcaa taacagatgc ctatggtact attaaagcta
     9061 ttgctgccaa cgatccggat aagacagtaa agttgcttgt taatagggca cagacagtta
     9121 ccgaagctaa aaaagttgct cagcgagtca ttaacattgc cgggcagttt ttgaacatta
     9181 aagttgataa tttaggtttt atctttgatg atctttatgt tgcaaaatcc atacgcaatc
     9241 aaaaaccatt tatagtgagc tatcctaaat caaaggcttc tacatgtgta gagattattg
     9301 cagataggat tggaaatatt gagtcagaca ttgataaagg aactggtatg ctaagctttt
     9361 tcagaagatt ttttggtcat tatgaaatgg ataatgatga tggtgttgta tagaattaaa
     9421 ttgatacatt gtgtatttat tacttgattt aagatgtatt taatactatt ttttatgtta
     9481 ggttgtaaat tttgtaaatt tgtgatagtg ttatctaaaa tggtcataaa tcatgttgaa
     9541 tattaagaaa attgcagaaa tttcaccgga agctaaagta tctctgatat ttggattcct
     9601 tgcatttata gtatctttat tctttgggct tgctgttggg aacagaattg ttatattatt
     9661 gaccagagta ttgtttttta caattgtcta tgcaacaatt ggttatgctt ccatgcttgt
     9721 gatcaagcat tttgttccgg aagtgtatga aatattaaac aaagtattga atattggtca
     9781 atcattagat atggctacag agtcatctga tgtgacgatg caatctgagt ataaaaacag
     9841 tgatgattct gataaagggg agggcattgt ttcttctgat tctggcaatg agtctattga
     9901 tgtgggggct acctataacg aagcatttac accattggaa gctaaggatt ttactacttt
     9961 tacaactgaa gaaagtaaac ctaaaaagat ggggaagcac atcgttctgg atgacaaaaa
    10021 gattcactat gaaccaaaaa taatggcaga agctatacga actatgatca agcgtgataa
    10081 ggactaaata aaataacgag gttgtatggc aaataaaaaa tttcaaggat ttgatgctac
    10141 agatgaagat gaactctgga aacggtatgc aaagacacaa aatcaggata tacgggacta
    10201 ttttgtaata aaatatgcac cattggttaa atatgtagcc ggtaaaattg ctatgggaat
    10261 gccaagctca atagaatttg atgatcttgt ttcctatggt gtatttggtc ttatagatgc
    10321 aatttcaaaa tttgatcctg atcgcgggat aaaatttaaa acatatgcga tgacacgaat
    10381 ccgaggggct atttttgacg aattacgttc aattgattgg ataccacgat caatcagaca
    10441 aaaagcaaaa cagattgagc aggctatcgc tgaattggaa aataaattgg gaagaactgt
    10501 tgaggatgaa gaaatagcca aagagttagg tgttaccatt gaagaatttc aatccttact
    10561 gaacaagttg agcgggacag ctatgttgtc gcttaatgat atatggtatg ttggggatga
    10621 aaatgatgaa ctgtcgataa tggaaacact tgaagcacct gatacattaa atcccgacgt
    10681 tatgattgag aaagaagaaa tacgcgacta tattgttgag gcaataaaaa aattacctga
    10741 taaagaaaag aaagttattg ttctatatta ttacgaggat ttaaccttaa aagaaattgg
    10801 tgaagttctt gaagtaactg aaagcagggt atcacaatta catactaagg cgataatgcg
    10861 attgcgtggc aggctgggaa ggattaaatc aaatattgtt ggatgaataa tctgatcagg
    10921 tattattata attaaaatgt atatctggtt tttgtataat gaagaatcta aaagaattgt
    10981 taaaaacatt aaaagatgat gatatagccg aaaatgaggt ggaagtatat gctgattcgg
    11041 tgaaacaggc tcttgaattg gcatccagag atctgggtgt tgatatatct gagcttgatt
    11101 atcagatttt ggagaaggga acatcaggtc tattaggaat cggcagacaa ccatataaag
    11161 tattgattag acctataaaa gtagaagagg aagaagattt aacagcgatt gaacaaaaac
    11221 tgcatgctgt acctgaagct gatttgaaag ttcaggatgt agcaagaaat gttgatggct
    11281 cagccaaaat tagaataacc aaaactggca tctggttaac agtaactccg ccgaaaggtg
    11341 gggggagacg tgttgatttg tccgatgtat caaataggat ttattctctt agaattcaaa
    11401 atgctgatat ggcaaaaata gagaaagaaa tcaaacatcc
//
LOCUS       DAOSOX010000071        11420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5661_length_11420_cov_8.502596, whole genome shotgun sequence.
ACCESSION   DAOSOX010000071 DAOSOX010000000
VERSION     DAOSOX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_11420_cov_8.502596"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(597..2024)
                     /locus_tag="PLX22_06605"
     CDS             complement(597..2024)
                     /locus_tag="PLX22_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT19606.1"
                     /translation="MALVYDKKDCFIVEGKLALPYSYFAGRVGSTFITTLRDKKKIMG
                     VKCPVCNKVYLPPRQTCERDLTDIRDNWVEVQPAGEVVNFTVVRYDDKYLPRKAPFVM
                     ALIKLDGADTPIVHILDGVSPEEVVIGMKVEAVFSSSPTNTILDIAHFAPKKPEKVYV
                     SQRKTGAKEKEGAQPISDEEKLKQERRKGMSKKVIITAALSGAATMKNQNQAVPYTPQ
                     EFAEESYKCYKAGAAMIHVHAREDNGMPTHDHKRIKDTHDAIKEKCPELIVNLSSAVG
                     MGKTPEQRISQILYVKPEMASLNTNTMNFSIVDRKTGKVFIDFIFENTFTMLQDFGKA
                     MEENNIKPEVEVYDLGGLDNWFLIAKQGFFTKPYNFNFVWGVAGGMEFRPDMFMVLKN
                     ALPEGANFTTCGVGTEQFPSINLSCMVGGHMRVGLEDNIRIPTGELAKGSYEQVEWAV
                     KIAQILGREPATPDEAREIMGLKKQ"
     gene            complement(2037..3218)
                     /locus_tag="PLX22_06610"
     CDS             complement(2037..3218)
                     /locus_tag="PLX22_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOT19607.1"
                     /translation="MEYWGVKVEDIFNTMHNRFRPEGAKGVVAEFGYDIIGEGKWKIK
                     VNDGELEVIKTDDISNCVAVMVCDGETFVGINIGKVDATNAFTSGKVKVEGDFGQFGK
                     TAKMFKKFVLKKAAMSVRDYILDMFGTLVQRFQPNNAPQEAIIVYDIGGEDGGVWTAY
                     IKDGKCELKEESPAKFSVKLEVQEAKDWVDVMLGKTDAFSLLSAGKGSIVGETQIALK
                     LEEIFSKYQDPMAGGTPEQELVVLKKIISVDQKFSTGPVMGKFLNALKNKKILGNKCP
                     KCGRIQLPAREVCAECRVRVDEWVEVGPKGQVRYMEYVYYASPDPLTGETRETPYGMA
                     NILLDGCKGNDTFAHLIRRDQIDLIKGGKDDVSGTRVRPVWNEHRTGSIFDIKYFEID
                     E"
     gene            complement(3228..4424)
                     /locus_tag="PLX22_06615"
     CDS             complement(3228..4424)
                     /locus_tag="PLX22_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT19608.1"
                     /translation="MAIEKNRNVAIIGIGQTKHSSHREDVNQPEMIYEAVSSALKDAN
                     LSIKDVDCVVHGNMELFEMIHQPDLWHVLGTGAYGKDSIRITTGGTVGATIACVSDNL
                     IASGMYDVVMAIGFEKLQEGHTTGGITNMADPLWFRNLQTGALTGSTAYDVMQEFGED
                     RAKRASMMYRIIMDKHACLNPNAHRAFGLEFDQIETLMNTSPQLVGELRLIHMCSQSD
                     GACAVILASEKKAKELSKKPVWIRDHITIHREETFNIFGYHQKYPVYKTHKFAAEQLF
                     KRNDIKQPLEYFDVFEMYDPASWWGLDWLRDFFFLKGDEHLKLVENKEIMIGGSMPIN
                     PSGGVIATNPIGATALLRVAEAALQIRGDAGAHQIPNNVKHALASGFGGTMWTVLMLL
                     EKELNW"
     gene            complement(4438..5583)
                     /locus_tag="PLX22_06620"
     CDS             complement(4438..5583)
                     /locus_tag="PLX22_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT19609.1"
                     /translation="MANRVAICAVAQIKNDPNLWHARFQNMLLECFEGIISQTNVGFD
                     MEKGIRTVLTCSDDVWDARTISDNAITDVVGAHFRGEEKIAQESLNGLGYAMACILSG
                     HDDIILYMGHMKESQTESRGMCTNLAFDPFYCRPLGMDFVNVNAMQARAYMATSGINE
                     EHLANIVVRSRKNAKKNPYARNNVLVKEDDVMKSPLLSDPLRELHYYPATDWAFGMLL
                     CNEKRAKEFTDKPVWMTGFANCIDSYFLGDKDLTSNFTLKNATERALKKAGIKGLKNN
                     FELYELCDHAAYQLPMWAEGIGLIKDGDGGKWISEGGLDECNINISGGMLNGNPLLLG
                     GAARVIECFYQLRGEADKRQVKGVKRALAQSTYGAAGQHQAVVIMEV"
     gene            complement(5635..6165)
                     /locus_tag="PLX22_06625"
     CDS             complement(5635..6165)
                     /locus_tag="PLX22_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT19610.1"
                     /translation="MHTHTTCSDGTLTPQQVALIYEEKGYDFIALTDHDYLLKPGFEK
                     IYNNVKTSLMLFSGIELTVFLKGYIHINRIFGDVNLLHIFNHLPEYDLDIGQIMNRLY
                     YLQKMFQIDAVEITAKGFKVDGFDTIHLPYHSIVSDDAHTEVGIGRAWIELDAKKNKD
                     SILRTIKHGDFWNCYC"
     gene            complement(6498..7538)
                     /gene="queA"
                     /locus_tag="PLX22_06630"
     CDS             complement(6498..7538)
                     /gene="queA"
                     /locus_tag="PLX22_06630"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT19611.1"
                     /translation="MKKKIYTLSDFDYFLPEELIAQYPAEHRGMSRLLIVNRKDGQLI
                     HDSFYNISHYLHKDDILIFNNAKVIPARIYCRRSTGSKVELVLTKHDTINNTWIAICN
                     RLKRLKAGEILYPIGNENINIIITEKLDDEIIIKTNPDLSNDILAKIGQLPLPPYIQR
                     APEPMDYERYQTVYAKKEGAVATPTAGLHFTEEILKQLHNKGVAIAFLTLYVSWGTFH
                     PVRHEDISQHTMHYEEYYLPPETVDAIYNAKSKGGRVIAVGTTSLRVLESVYKEGEYI
                     SGYGETNLFIYPPYTIQSVDALVTNFHTPKSTLLMLVSAFAGYDCIMNAYDEAIKHHY
                     RFFSYGDAMLII"
     gene            complement(7605..8747)
                     /locus_tag="PLX22_06635"
     CDS             complement(7605..8747)
                     /locus_tag="PLX22_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT19612.1"
                     /translation="MYVIIALLVMIGCVPSPEYLAKTGKRVGLDNDYIRVLLLKDQVK
                     VIIETTSKTKVVQLSNGTILYNGNPKHLEYFIHKLPETLQFETWNNEIIVNGIKVRGM
                     IQLIPVVGKIQVVNVLKMNEYLYGVLPSEIMPGWPIETLKAQAVAARTYAYYHIMKNN
                     STYYDLDASTNFQVYRGAISETETTNKAVDSTSGIIMVYNSIPILAYFHSTCGGRTAD
                     DRFVWKGEDLPYLTSVNCSYCKNSPVYSWQVNISLDEIYEALLKKYKTVGQIKAITLG
                     REDNRVSNIKIEHSNGIIRMTGNDFRMMFETKKIKSLYFEANQTHDGLTLNGHGWGHG
                     VGMCQWGAKEMAGRGKNFYDILKYYYRGISVVSINTVPQKYRKSNF"
     gene            complement(8789..9679)
                     /gene="xerD"
                     /locus_tag="PLX22_06640"
     CDS             complement(8789..9679)
                     /gene="xerD"
                     /locus_tag="PLX22_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT19613.1"
                     /translation="MKDIIAIKRFKRFLQTEKGLSPNSIYSYIYDLKKFSDFLANKNK
                     TILNASQEDIEQFLFFEKNKKRNSARTLARSLAAIRQFYNYMANIEHIANPTVKIETP
                     HFQKALPDFLSIEEIDRLFSSIREDDMYQLRDKTIFELLYSAGLRISEAVELKAVDVD
                     LKNKFIKVMGKGNKERIVPIGDEAIRLIEKYMRDSRPVILGSRMSEYLFISKKGGMLN
                     RKSVWRLLKGYAQMVNIRKNITPHTLRHSFATHLLERGADLRAVQELLGHVDISTTQI
                     YTHLARKQLQEIHKRFHPKG"
     gene            complement(9852..11030)
                     /locus_tag="PLX22_06645"
     CDS             complement(9852..11030)
                     /locus_tag="PLX22_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT19614.1"
                     /translation="MDFSNLLITALRALAKNKVRSILTSIGIVIGVSSVIVMIGIGQS
                     ASIAVEERVGTYGKNAMEIASYRNLLTENDSIYIKNNIPQVNYVSIVITKSNVQINYK
                     DRALMSALYGVENDYFKMKIWPLFSGRYFTDMEIDTHERVVIIGSTVMREIFLYEEPI
                     GKTVIINSVPFKVIGVLSELGQSFGGKDFDNIVIMPYGTVQRKIEGNRKIDRIYVSVY
                     SEQQLDETIKYIRDYLHRVHRIPPDVNEFRITTSKERLEMAEYISKTLAMLLAGIASI
                     SLFVGGVGIMNIMLVSVSERTREIGIRMAIGAKRRDILSQFLIESITLSGLGGIIGIL
                     LGLFFYYIITYIVDWPYIFSFTSVIISFLFSCAVGIFFGYYPAKKASDLKPIEALRYE
                     "
     gene            complement(11032..>11420)
                     /locus_tag="PLX22_06650"
     CDS             complement(11032..>11420)
                     /locus_tag="PLX22_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003573209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT19615.1"
                     /translation="PLFYRGGFSHEEMRERAGKFLAMVGLSGRETHHPNKLSGGEQQR
                     VAIARALVNDPAILLADEPTGNLDSKNTSEIMNIFTYLNKEYNLSIVMVTHEPDVAEY
                     ADRKVIFKDGLIIDEVIIKKLKKRKK"
BASE COUNT         3547 a         2400 c         1549 g         3924 t
ORIGIN      
        1 aaactacagc aacattttct ttttctatct gataataaca tttatcattc ataattcaat
       61 cctgaatttt aattgcatat agtaaaagta cgcattatct catccatacc tatgtaatat
      121 gcatccctgt cattccatgc ttaatctgga atctcaacat gtaacgttgt atcctgaaat
      181 aaattcagga tgacgtccta ctcaacgtca taccgggctt gacccggaat ctacattgta
      241 atcgtcaata ctgatagtat aatgtcatcc ttacggggtt tgtcatgcaa cctattttca
      301 ttccttatag gtggttgcac gctggccatg aacaacttct taactaaaac catcatcatt
      361 aatggtatgc taacagataa aaaccatatg cttccattaa ccatttttaa aatcatcgtt
      421 actcaaaatt cccaaactct atcaaatcac atctcctcat caaatagcta atgtcatcac
      481 atcattggta taatcgtaat ttctcaatgt gccagtagtt tacaaataac tatcgtacaa
      541 aatagatatt ccctgcatct atccgtgata ataagcagat gcagggataa gaaatattat
      601 tgttttttca gacccataat ttctcgtgct tcatccggag tagctggttc tctacccaat
      661 atttgtgcaa tttttaccgc ccattcaacc tgctcataac ttccctttgc caattcaccg
      721 gtaggaattc taatattatc ttcaagacca acccgcatat gtcctccaac catgcatgac
      781 aaatttattg aagggaactg ttctgttcca acaccacatg ttgtaaaatt tgcaccctcc
      841 ggtaatgcat tcttcaaaac catgaacata tcaggcctaa attccatgcc tcctgcaaca
      901 ccccatacaa aattgaaatt atatggcttt gtaaaaaatc cctgcttagc tatcaaaaac
      961 caattatcaa gcccaccaag atcatatact tccacctcag gctttatgtt gttttcttcc
     1021 attgctttgc caaaatcctg taacatcgta aatgtatttt caaatataaa gtctataaat
     1081 actttaccag tttttctatc gacaatactg aagttcatag tattggtatt cagtgaagcc
     1141 atctcaggtt ttacataaag tatctggctt atacgttgtt caggtgtttt acccataccc
     1201 actgctgaac ttaaattaac aatcaactcg ggacattttt cttttatagc atcatgggta
     1261 tctttaatac gcttatgatc atgtgttggc ataccattat cttcacgagc atgtacatga
     1321 atcatagctg cacctgcttt atagcattta tatgattctt ctgcaaactc ctgaggtgta
     1381 tagggcactg cttgattttg attcttcata gttgcagccc ctgataatgc tgctgtaata
     1441 ataacctttt ttgacattcc tttcctcctt tcttgtttta atttttcttc atcagaaatt
     1501 ggttgagccc cctctttttc ttttgcacct gttttgcgtt gggaaacata aactttttct
     1561 ggtttctttg gtgcaaaatg tgcaatatcc agaatagtgt tcgttggtga agatgaaaac
     1621 actgcctcaa ccttcatccc tatcacgact tcttccggtg aaactccatc aaggatatga
     1681 actattggtg tatcagctcc atccagttta attaaagcca taacaaatgg tgctttgcgt
     1741 ggtaaatatt tgtcatcata acgcactaca gtaaaattaa ccacttcacc agcaggctgt
     1801 acctctaccc aattgtccct tatatcagtt aaatcacgtt cacatgtttg tctgggcggc
     1861 aaatatactt tattacaaac gggacatttt acccccatta tctttttctt atcccgtaga
     1921 gttgtaataa aggtactacc tactcttcct gcaaagtaag aatatggtaa agctaatttt
     1981 ccttcaacaa taaaacaatc ttttttatca tatacaagtg ccatatcgtt cctccatcat
     2041 tcatcaatct caaaatattt tatatcaaag atgctccccg ttctgtgctc attccataca
     2101 ggtctaacac gtgtaccgct aacatcgtct tttccacctt ttatcaggtc tatctgatca
     2161 cggcgaatta aatgtgcaaa tgtatcatta cctttgcatc catctaataa tatatttgcc
     2221 attccatacg gtgtctccct ggtttcacct gttaacgggt caggacttgc atagtacaca
     2281 tattccatat agcgaacctg acctttaggg ccaacttcaa cccattcatc tacacgcaca
     2341 cgacattcag cacatacttc acgtgccggc aattgaatac ggccacattt tgggcatttg
     2401 tttcccagta ttttcttatt ctttaatgca tttaaaaatt tacccattac tggacctgtt
     2461 gaaaattttt gatcaactga aataattttt tttaatacaa caagttcctg ctcaggagtg
     2521 ccgccagcca ttggatcctg atatttagag aatatctctt ctaatttcaa cgctatctga
     2581 gtttcaccaa ctattgaacc tttccctgca gaaagaagtg aaaatgcatc tgttttaccc
     2641 agcattacat ccacccagtc tttagcctcc tgtacttcta atttcacgga aaattttgca
     2701 ggtgattctt ctttcaattc gcattttcca tctttgatat acgcagtcca tacaccacca
     2761 tcttcaccac ctatatcata tactataatt gcttcttgtg gtgcattatt gggttggaaa
     2821 cgctgtacca atgtaccaaa catatctaaa atataatctc taacagacat agcagctttt
     2881 tttagtacaa attttttaaa catctttgct gtttttccaa actgaccaaa atcaccttct
     2941 acctttacct tacctgatgt aaaagcattt gtagcatcaa ccttgcctat attaattcca
     3001 acaaacgttt ccccatcaca taccataact gcaacacagt tactgatatc atctgttttg
     3061 ataacttcta actcaccgtc attaactttt attttccatt taccttcccc aatgatatca
     3121 taaccaaatt cagcaactac tcctttggca ccttcaggtc taaacctgtt atgcatagta
     3181 ttaaatatat cttcaacttt aactccccaa tattccatag caccctccta ccagtttaat
     3241 tctttttcta gtaacataag aaccgtccac atagtcccac caaaccctga agccaaagca
     3301 tgtttaacgt tattgggtat ctgatgcgct ccagcatctc ctcttatctg caaggcagct
     3361 tcagcaacac gcaataatgc agtagctcca ataggatttg tagcgatgac tcctcctgat
     3421 ggatttattg gcattgatcc tccaatcatg atctctttat tttccaccag ttttaaatgt
     3481 tcatcccctt ttagaaaaaa gaaatcacgc aaccaatcta aaccccacca tgaagctgga
     3541 tcatacatct caaaaacatc aaaatattcc agtggttgtt taatatcgtt ccgtttgaac
     3601 aattgttcag cagcaaattt atgagtttta tatacaggat atttttggtg atagccaaat
     3661 atattaaatg tttcctcacg gtgtatggta atatgatcac gtatccaaac aggttttttt
     3721 gatagctctt ttgctttttt ctcactggct aatataacag cacatgcacc atcagattgc
     3781 gaacacatgt gtataagcct gagttctcca accaattgag gagatgtatt cattaatgtt
     3841 tctatctgat caaactcaag tccaaatgct ctatgagcat tgggatttaa gcatgcatgc
     3901 ttatccataa taatacggta catcattgaa gcacgctttg ctctatcctc tccaaattcc
     3961 tgcataacat catacgctgt tgaaccagtt aatgctccgg tttgcagatt cctgaaccat
     4021 aatggatcag ccatattggt aatgccacct gtagtatgtc cttcctgtag tttctcaaag
     4081 ccaatagcca taacgacatc atacatcccg gaagctataa ggttatcact aacacatgct
     4141 atcgttgctc caactgttcc tccagttgtt atgcgtatgc tatccttgcc atatgcaccc
     4201 gtacctaata catgccataa atcaggctga tggatcattt caaatagctc catattccca
     4261 tggacaacac aatcaacatc cttgatactt aaattagcat ccttcaatgc agatgaaaca
     4321 gcttcataaa tcatctccgg ctgattaaca tcctctctat gacttgaatg ctttgtctgt
     4381 ccaataccta ttattgccac atttctattt ttctctattg ccatgagctt acctcctcta
     4441 aacttccata attacaacag cctgatgctg accggctgcc ccataggtac tctgtgccaa
     4501 tgcacgtttg acacctttga cctgtctttt atctgcttcg cctcttaact gataaaagca
     4561 ctctataact ctcgccgcgc ctcctaaaag caatggattc ccatttaaca ttcctcctga
     4621 aatgtttata ttgcattcat ccaatccgcc ttcacttatc catttaccac catctccatc
     4681 ttttattaaa ccaataccct ctgcccacat aggcaattga tatgctgcat gatcacataa
     4741 ttcatataac tcaaaattgt tttttaatcc tttgatcccg gcttttttta atgcacgttc
     4801 agttgcattt tttagagtaa aattggaagt taaatcttta tcgcctaaaa aatagctatc
     4861 aatgcagttg gcaaatccag tcatccatac cggtttatct gtaaattctt ttgctctttt
     4921 ttcgttgcac aaaagcatcc caaaagccca atcagtcgca gggtaataat gaagctctcg
     4981 caaaggatca gacaacaaag gggatttcat gacatcatct tctttaacta atacattgtt
     5041 tcgtgcataa ggattttttt tcgcattttt tcttgaacgt acaacaatat tagctaaatg
     5101 ttcttcattg ataccagaag tagccatata cgcccttgcc tgcatagcat taacatttac
     5161 aaaatccatt cctaatggtc tgcaataaaa tggatcaaat gccaaattgg tacacatacc
     5221 cctgctttca gtttgtgatt ccttcatatg ccccatgtac aaaatgatat catcatgccc
     5281 ggaaaggata catgccattg cataccccaa accattcaaa ctttcctgtg caattttttc
     5341 ttcacctctg aaatgagccc caacaacatc agttattgca ttatcagaga tagtacgcgc
     5401 atcccaaaca tcatctgagc atgttaaaac agtgcggatt cccttttcca tatcaaaacc
     5461 aacatttgtc tgggatatga taccttcaaa acattccaat agcatgttct ggaaacgagc
     5521 atgccaaaga ttaggatcat ttttgatttg agcaacagca cagatagcaa ctctatttgc
     5581 cataacccac gaacctccat ataaaaattt ataatactta aactatatca tactttagca
     5641 atagcaattc caaaaatcac catgcttgat agttcttaat attgagtctt tatttttttt
     5701 tgcatccagt tcaatccatg ctcttccaat accaacctct gtatgagcat catctgaaac
     5761 aatagaatga tagggtagat gtattgtatc aaatccatcc actttaaatc ctttagccgt
     5821 tatctcaact gcatctattt gaaacatttt ctgaagataa tataacctat tcataatttg
     5881 tccaatatcg agatcgtatt ctggtaaatg attaaatata tgtagtaagt ttacatctcc
     5941 aaatattctg ttaatatgaa tatatccttt taaaaatact gttaattcta ttcctgaaaa
     6001 aagcatcaaa gaggttttga cgttattgta tattttctca aatccaggtt ttaaaagata
     6061 atcatgatca gtaagtgcaa taaaatcata acccttctcc tcgtaaatga gagctacctg
     6121 ttgcggtgtt agcgttccat ctgaacatgt agtatgagtg tgcaaacatc ctttcattaa
     6181 cattttatta taatatcaga ataaatattt gaatggacat tcaatcaggt aataagttac
     6241 tttaattgga catccaatca attatcaagt tacagaagac ataaaaatat gtcaacagtt
     6301 tttttaatta ttttttattt ttaaagggta actgctcaaa aaatgcaggt aaataaaaaa
     6361 agactattaa ttttaatgag tactattatc atgtccccat ggacataaag atacaaatac
     6421 ctgaaatacc tgaaatacct gaagaagagt taacgccgac agtgagagtg ttgcttgcta
     6481 taattttttt tatttcttta tattatcaac attgcatcac catacgagaa aaatcgataa
     6541 tgatgtttta ttgcttcatc atatgcattc atgatacaat catatccagc aaatgctgat
     6601 acaagcatta acaatgtaga ttttggagta tgaaaattag ttactagtgc atccactgat
     6661 tgtattgtgt aaggcggata aataaataaa tttgtttctc catatcccga aatatattcc
     6721 ccttctttat aaacagattc aagaactcgc aaagaagtag ttccaacagc tattactcta
     6781 ccacctttag atttagcgtt ataaatggca tcaactgttt ctggaggaag atagtattcc
     6841 tcataatgca tggtatgttg tgaaatatct tcgtgtcgta caggatggaa tgtaccccat
     6901 gaaacatata atgttagaaa agcaatagca acccctttat tgtgtaattg tttcaatatt
     6961 tcttcagtaa aatgtaatcc agcagttggt gttgccactg caccttcttt tttagcatac
     7021 accgtttgat aacgctcata atccataggt tcaggagcac gttgtatata cggaggcaga
     7081 ggcaattgac caatttttgc taatatatca tttgacaaat cgggatttgt tttaataatt
     7141 atctcatcat caagtttttc tgttatgata atatttatat tttcattacc tattggataa
     7201 agtatttcac ctgcttttag tcgttttaac ctattgcaaa ttgctatcca tgtattattt
     7261 attgtatcat gttttgttaa aactaattca acttttgaac cagttgaacg tctacaataa
     7321 attcgagcag gtattacttt tgcattgtta aaaataagaa tatcatcttt atgcagatag
     7381 tgagatatat tataaaatga gtcatgtatt agctgaccat ccttccggtt aacaattaat
     7441 aatcgcgaca ttccacgatg ttctgctgga tattgagcaa taagttcttc tggaagaaaa
     7501 taatcaaaat cactaagggt atagattttt tttttcatag gacccaacaa ttagaaaaca
     7561 taattatttg caagtatatt tgtttatcaa tttaaaccag catattaaaa attagatttt
     7621 ctatattttt gtgggactgt atttattgat acaactgaaa taccgcgata ataatatttt
     7681 agaatatcat aaaaattttt acctcgtcct gccatttctt ttgctcccca ttgacacatc
     7741 ccaacaccat gtccccaacc atgtccattc agtgtaagcc catcatgagt ttgatttgct
     7801 tcaaaatata aactttttat tttttttgtt tcaaacatca ttctaaagtc atttcctgtc
     7861 atccgtatta taccattcga atgttctatt tttatatttg atacacgatt atcttctctg
     7921 cctaaagtaa tagctttaat ctgaccaaca gtcttatatt tttttagtaa tgcttcatat
     7981 atttcatcta aagagatatt gacctgccag ctatagactg gggagttttt acagtatgaa
     8041 caatttaccg aagttagata cggtaaatcc tcacccttcc agacaaatct atcatcagct
     8101 gttctaccgc cacaagttga atggaaatat gctaaaatag ggatactatt gtaaaccata
     8161 atgatacctg atgtactatc aacagcttta tttgtagttt cggtttctga aatcgccccc
     8221 ctataaacct gaaaattggt tgatgcatct aaatcataat atgtactgtt atttttcatg
     8281 atatggtaat atgcatatgt tcgtgcagca actgcctgtg cttttaaagt ttctattggc
     8341 caaccaggca tgatttcaga tggcaacaca ccataaagat attcattcat ttttaaaaca
     8401 ttcaccacct gtatttttcc aacaacagga attagttgaa tcattcctcg tactttaatt
     8461 ccgttaacaa taatctcgtt attccatgtt tcaaattgca aagtttctgg taatttatgg
     8521 ataaaatatt ccaaatgttt tggattacca ttataaagaa tagtaccatt actcaattgc
     8581 acaacttttg ttttggaagt agtttctatt ataactttta cctgatcttt caataaaagc
     8641 actcttatat aatcattatc cagtccaacc cgttttcctg ttttagcaag atactcaggg
     8701 gatgggacac atccaatcat aactaaaaga gcaattatta catacaatcg catcttacat
     8761 attgtgggca taccaagaag catacctgtt atccttttgg atgaaaacgt ttatgaattt
     8821 cttgcaattg ttttcgtgct agatgagtat aaatttgtgt tgttgaaata tctacatgcc
     8881 ctaataattc ctggactgct cttaaatcag cacctcgttc taaaagatgt gttgcaaatg
     8941 aatgacgcaa tgtatgtgga gtaatatttt ttcgtatatt taccatttgc gcataccctt
     9001 ttaataatct ccatactgat tttctgttta acataccacc ttttttgctt atgaaaagat
     9061 attcactcat cctgctacct aaaataacag gtcttgaatc tctcatatat ttttctatta
     9121 aacgtatagc ctcatctcca attggtacaa tacgttcctt attccccttc cccataacct
     9181 taataaattt attttttaaa tcaacatcta cagcttttaa ctcaactgct tcagatattc
     9241 tcaatcctgc tgaatataga agctcaaata ttgttttgtc acgtaattga tacatatcat
     9301 cctcacgtat tgaagaaaac aacctgtcta tctcttctat agataaaaaa tcaggtaatg
     9361 ctttctgaaa atgaggagtt tcaattttca ctgtaggatt ggctatatgt tctatatttg
     9421 ccatataatt ataaaactgc cttatcgctg ccaatgatcg tgcaagtgta cgtgctgagt
     9481 ttcttttttt atttttttca aaaaacaaaa attgctctat atcctcttgt gaagcattaa
     9541 gaatagtttt atttttattt gctaaaaaat ctgaaaactt ttttaaatca tagatataag
     9601 agtaaattga attaggagat aacccttttt cagtttgtaa aaaccgttta aaccgtttaa
     9661 tagctataat atctttcaat tgatatcacc tcttcttaat acttcccctt gattcgcaat
     9721 ttgttaatcc aggttatgta ataaaatcaa aacataaaca tatagtttaa aaatttttta
     9781 tgtcgtatat aatatcggaa aaaagaggat tatgtcttga agggatttga gaaaaaaatt
     9841 ataaatacta cttattcata tcgtaatgct tctataggtt ttaaatcaga tgcttttttt
     9901 gcaggatagt aaccaaagaa tataccaaca gcacatgaaa ataggaatga tattataact
     9961 gatgtaaaag aaaaaatgta aggccaatca actatataag taattatata atagaaaaac
    10021 aatcctaata gtatacctat tatacctcct aatccactca gtgtaataga ttcaattaaa
    10081 aactggctta atatatcacg ccgttttgct cctattgcca ttcgtatacc tatctctctg
    10141 gtacgctcac tgacagagac aagcatgata ttcataatcc ccacaccgcc tacaaatagt
    10201 gatattgaag cgataccggc taaaagcatt gccaatgtct tggaaatata ttcagccatt
    10261 tccagtcttt ctttgcttgt tgtaattctg aattcattga catctggagg tattcgatga
    10321 actcgatgta aataatcccg tatatattta attgtttcat ccaattgctg ctcagaatat
    10381 acagagacat atatacgatc tatttttcta ttaccttcaa ttttcctttg aactgttcca
    10441 taaggcatga tgacaatatt atcaaaatct tttccaccaa atgactgtcc caattctgaa
    10501 agaacaccta ttactttaaa tggaacacta ttaataatca ctgtcttacc aattggctct
    10561 tcatataaaa aaatctccct cattaccgtt gaacctatga taacaactct ttcatgagta
    10621 tcaatttcca tatcggtaaa ataccttcct gaaaacaatg gccatatttt cattttaaaa
    10681 taatcattct caacaccata taatgcactc atcaatgcac gatctttata atttatctgt
    10741 acatttgatt ttgtaataac aattgaaaca taatttacct gaggtatgtt atttttgata
    10801 taaatactgt cattttcagt aagcaaatta cgatatgatg ctatctccat agcattttta
    10861 ccataggtac ctaccctttc ctctacagct atactggcac tctgccctat accaatcata
    10921 acgataaccg atgaaacacc aattacaata ccaatgctcg taagaataga gcgaacctta
    10981 ttctttgcca gcgccctgag cgctgttatt aacagatttg aaaaatccat gttacttttt
    11041 tcttttcttc aactttttta taattacttc atcaataata agcccatctt taaaaataac
    11101 cttcctatca gcatactctg caacatcagg ttcatgcgtt accataacta tactgagatt
    11161 atactcttta tttagatagg taaaaatatt cataatttca gaggtatttt ttgaatcaag
    11221 atttccggta ggttcatccg ccagtaaaat agcaggatca ttaaccaatg ctctggctat
    11281 agctactctt tgctgctcac cacctgacag tttatttgga tgatgcgttt cccttccgga
    11341 caaccctacc atagccaaaa atttgcctgc tctttctctc atctcttcat gagaaaatcc
    11401 acctctataa aatagaggca
//
LOCUS       DAOSOX010000072        11388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5676_length_11388_cov_9.248301, whole genome shotgun sequence.
ACCESSION   DAOSOX010000072 DAOSOX010000000
VERSION     DAOSOX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5676_length_11388_cov_9.248301"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1283)
                     /locus_tag="PLX22_06655"
     CDS             complement(60..1283)
                     /locus_tag="PLX22_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOT19616.1"
                     /translation="MATIKEFTALRPGKDVVTKVASLPYDVVTAQQAKKIAQNNPCSF
                     FYVTMPEIDLPLSTNPYSDEVYKLGKKKLSKFITDGIMVQDDKPYLYLYTLQIDSHKQ
                     TGIVAVVSIDDYINGIVKKHELTRKAKEMDRMNHINIVGAQTGLVYLFYKDSNDLRNI
                     MHHALSSPVLYDFVADDGVRHIIQRIEDGDYAQLVKQAFDEQILYIADGHHRAASAVR
                     VGLQRREQGYTGSEPFNYFIAAIFPHSELNILPYNRVVKDLYNHDFETFMRLLSDSFV
                     VQKTDNGIAKNRHEIRMYYDHQWYTLTFIKKLPLDAVDALDVSILQNTILSPILGIKD
                     PREDMRIDFVGGTNAIATMVELVDSGSYVIGFSLFPTSIDELMTISDRGEIMPPKSTW
                     FEPKLRDGLLVYCID"
     gene            complement(1303..1791)
                     /gene="greA"
                     /locus_tag="PLX22_06660"
     CDS             complement(1303..1791)
                     /gene="greA"
                     /locus_tag="PLX22_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT19617.1"
                     /translation="MAVNIADKKKQVKEEIEKLQHELKIELPKRIAEARSKGDLKENA
                     EYHAARERQSFVQAQISYLSKQLSLIDSIDISTIEQGKVDFGSVVTVRNLDNDEVARF
                     TIVTPDEVDASEGKISLSSPIGRALHKKTVGDEVSVIIPAGTKRFRIEKLVTIHGEEL
                     SL"
     gene            complement(2154..2579)
                     /locus_tag="PLX22_06665"
     CDS             complement(2154..2579)
                     /locus_tag="PLX22_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOT19618.1"
                     /translation="MDTECGMVKEVRGNLVCVAADPASLCATCSSQCMMRQKGRDIWI
                     QNTLDAKVGDRVYFVFPPGAVVLSSGVLYGIPIVLLIAGIVAGTLYPLPFFNDRELSA
                     IVMGIIFLALSFVFIQIVSQYIAAKNMVNPVMVKVEKAV"
     gene            complement(2766..4172)
                     /locus_tag="PLX22_06670"
     CDS             complement(2766..4172)
                     /locus_tag="PLX22_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19619.1"
                     /translation="MSELKRCEDSVRQCSGVLFFLFFFLLFAADCMATIAVEGLTVNV
                     DIQDIINQGVNNQKEAEYWAIVITNRLHSQGYTTSYVAHYELRDDVCTLYIVESHIAD
                     IQVHTDAYIQDALTQDVTALLGSVYNKHTVYNLLAQLKQKYMLRSINVNVVNYNNGSD
                     VSLIIEAKPEHFIYSFTIAMLPIYGIKPAITVTMPMQDAYCSVKGQMAFDDQRITLKQ
                     GGFDYMNYRSKAAWHTGIDAQEEYGVWERYSRSFTLTQCRAYLGLGTIMEAGMYDVSA
                     FIYGVAAYYSMSELENDVSHDAGAELRITATDQRHSIVYEKSMVTLSFIAAYANEAFI
                     TSRIAGSVPIQITSRFYCIPNIYSFHTTSSERIYAEYVFDSYLLGYSDRYTSTHSRHI
                     VGMQLVYEALYELLYCKIFGSAGIYKDEYAAWDNTASYGFAVDIVYGRIVATVGCAWN
                     VEEHFDTCYFFTGVKARW"
     gene            complement(4150..4851)
                     /locus_tag="PLX22_06675"
     CDS             complement(4150..4851)
                     /locus_tag="PLX22_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19620.1"
                     /translation="MKNLVLVVLLLLSGQTDTDELQKLFHNFNVQSIDASIVQVIAET
                     GKETQRYEGRYRAYNDCMRIDYSKPFRQIVIVNGGKLLWYYPETKELWTMSQGNVPAV
                     HPYAQCDIVNERIVVKSKKKVWYGFFNSGYRYTLFDSKTGVTLELTIDKDKGYPVKKI
                     TYTAQSIEVMRELYSDYTLIQGIWFPATVEVIARTHTGTTRNVTRYYNIILNGNVSTD
                     IFTLQLPKGVRAKTM"
     gene            4938..5663
                     /locus_tag="PLX22_06680"
     CDS             4938..5663
                     /locus_tag="PLX22_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19621.1"
                     /translation="MRYLSILIILFLTCTGAFAQINTAAQPLQYPGATISTQTPYFIW
                     CDIYNTAAKPFVVKLTLTDSRGQSNEFTLTPLTVDSIYCVATIPVVLTPDTYTYTIHL
                     LSNNKPVNSRYYHHKRYPVKGSFTIDVSRHNAVDTLNANELVYYLSQSRSNMLHNGYN
                     AMFFSASGIISTCAGVAVYYWTNFGIVTTIISAITITSGVIGISAGIYYGMQYYHNEA
                     TINKVVTHDTVPQPHLAPDAGKQ"
     gene            5826..9215
                     /gene="mfd"
                     /locus_tag="PLX22_06685"
     CDS             5826..9215
                     /gene="mfd"
                     /locus_tag="PLX22_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT19622.1"
                     /translation="MVLKKLTQIYASTDECKPLLASRGAFSIEGIHPSTFPFIVATAF
                     NHAPSQIIAVTADYHAMNQFIACLSAFTSEEHIFPYPPLEVLPYEMVNPSSAIERDRI
                     TALYQLLSGNPVIVVATVESLLRAIPHKEFFLKKGITIHKGEEYPFEVILETLASYGY
                     ERETRVDRFGQFSVKGGIIDIFLPNCTNPVRCDFFGDTCESIRYFDPVTQVSLTETDA
                     ITIYPRRELLLSPSQKEQLKKALQPVGDSSLQKQLDSMEKQSIPGIYDLFPIIIPGTT
                     ILSIATQPTVFLCDPSQLKAKLAELERTYHQLYMHKKDMAVFPHDSQILLGDSYPEIE
                     KQAVRLQPFIADSSLVWQLKSIPSFQGRIADVRSECSKRIDEGWHIIVSTSFEGQARR
                     LYDLFGEFHPEDTFDAFNPDKQFTIVISNLHEGFEISAIKTLIITDHEIFGKSYRKKK
                     HFKQKYSRPIDTFLELSPGDYVVHINHGIGVFKAIERMSAGGVERDFIVIEYADGDKL
                     YVSLDQINMVQRYVGLDGRQPRIDALGKKSAWNRIKQRVQESVEEIAHDLLKIYSQRK
                     ALKGYQFPPDTIWQEEFESKFEYEETPDQITAIEDVKDDMEKSQPMDRLVCGDVGFGK
                     TEVAIRAAFKAVMAGKQVAILVPTTVLAMQHYNTFIKRFADYPVTIDMLSRFKTQQQI
                     KSIKEKIKTGTVDIVIGTHALLAKDVSFKNLGLVVIDEEQHFGVKHKEYLKKLRLLVD
                     VITLSATPIPRTLHMALAGIRDLTIIATPPESRQSIETLVIEDNPDIVRMAIQNEIQR
                     KGQVFFVHNRISTIYEQAQYLEKLVPEATFCVAHGRMHEHELEEVMVDFINGRYDVLV
                     STSIIESGLDMPNVNTIIINRADTMGLSQLYQLKGRVGRSDRQAYAYLFYPKNAPITE
                     DAQKRLQVIAEYTDIGSGFKIAMKDLEIRGSGNILGREQSGNIMDVGFDLYCQLLEDT
                     VRNLKGEKPVHIFRTPVYVKTDIFIPDSYIADEKQKIEFYKRYEACETLDELNKLTRE
                     MIDRFGQPPKEVVSLIAMEEVRTLASQLYIDEIIEDTKLIKMRITRESKLDVKKLVTL
                     IQKDKRISIDRYDKEVVNVVTGIIPPEKKLEEIKKLLQQLS"
     gene            9316..10002
                     /locus_tag="PLX22_06690"
     CDS             9316..10002
                     /locus_tag="PLX22_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOT19623.1"
                     /translation="MKTKFLYCAIAIMVLAMVISCGNQYGGKWIAKIDDETITDNELN
                     AYYYAQMKSIYNLPKEEIDKLAQDPAQLERNPILNKNNFLEQMIQQRLVYKKAIDDGI
                     LKNEELQTLLDISKEGLVVQYYIREKFKNDISIAPQEVEMIYNQQRARFKGVPIDQAE
                     MYIKQQLFQQKLNMKIKELVDTLRDEKKIEKNMDLLRKELNTQSPVQQAPQQEHVPQP
                     QQQMQNPPAK"
     gene            complement(10127..10594)
                     /locus_tag="PLX22_06695"
     CDS             complement(10127..10594)
                     /locus_tag="PLX22_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19624.1"
                     /translation="MNKRRMLILFFLVLFIASGCKTVPKYEPPIEPIISPTEVNVNFR
                     FIAYDTGITTPDEDKRCYYKIFIDKIDAGRTTIGLESQKKYFEAKLSSNMHLIVVEKW
                     VLDEREGEYKKVNNILQPKPNFFYFETKASSLTELIMINDKMVNKAQFTVTLQ"
     gene            complement(10591..>11388)
                     /locus_tag="PLX22_06700"
     CDS             complement(10591..>11388)
                     /locus_tag="PLX22_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19625.1"
                     /translation="AGNYYIMVAAKNYNANNDVPYSLICTTRNYDNKTEIEPNNIMES
                     ATPMVTEIIYGRIYPDNDIDYYQYMGDSSNDVFYRIEINPPMTLDVVSAVYTKKGEEI
                     TSINNNGTGDKEVYPNVKLNAPFYVKVWAKRGQVDMDNSYALRIVPVQMGNLVDSEPN
                     DTKEQANNVQGTLINGYISKKHDIDFYVLHYKGRQKKYFSIKAPDTIPISFSVTDSLG
                     YIIQSITVKAGSSNTLHEIVDMKGYCIVKPLNEMYDGLYTIEIKDRP"
BASE COUNT         3533 a         2427 c         2125 g         3303 t
ORIGIN      
        1 gagagcgctt gaatggcatt caagaggtca ggggttcgat tcccctcgtc tccaaggcgt
       61 taatcaatac aatataccag caagccgtcc cgcaattttg gttcaaacca ggttgacttg
      121 ggcggcatta tctcccccct gtctgatatt gtcataagct catcaattga cgtagggaaa
      181 agcgaaaaac caattacata gctgccacta tccaccaatt caaccattgt agcaatagca
      241 ttggttccac ctacaaagtc tatacgcata tcttcccgtg gatcttttat tcccagaata
      301 ggactaagta ttgtattttg caggatagaa acatccaatg catcaaccgc atccagtggc
      361 aattttttaa taaaggttaa tgtataccat tgatgatcat agtacatccg tatctcatgc
      421 cgatttttcg caataccatt atcagttttt tgaacaacaa acgaatcaga tagaagtcgc
      481 ataaaagttt caaaatcatg attatataaa tctttcacta cacgattata cggcaaaata
      541 tttaactcag aatgaggaaa gatagctgct atgaaataat taaacggttc gctgccagta
      601 tatccctgct cccggcgttg caatccaacc cttacagccg aagctgcgcg atgatgacca
      661 tcagcaatat agaggatttg ttcatcaaaa gcttgtttta ccaattgtgc gtaatcacca
      721 tcttcaatcc tttgaattat gtgccgcaca ccatcatctg ctacaaaatc ataaagtaca
      781 ggtgatgata atgcatgatg cattatattc cgtaaatcat tgctatcctt ataaaaaaga
      841 tacaccagcc ctgtttgtgc cccaacaatg ttgatatgat tcatcctgtc catttctttg
      901 gctttacggg taagctcatg tttttttact atcccattta tataatcatc aatggacaca
      961 acagcaacaa tacccgtttg tttgtgacta tcaatctgta aggtatagag atataaatag
     1021 ggcttatcat cctgcaccat tatgccgtca gttataaatt tgcttaattt ttttttccca
     1081 agtttatata cttcgtcaga ataaggattg gttgacaatg gtaaatctat ctcgggcatt
     1141 gttacataga aaaagctaca gggattattt tgagcaattt tttttgcctg ctgtgctgtt
     1201 acaacatcat acggaagtga tgcaaccttt gtaacaacat ccttccccgg ccgcaatgcg
     1261 gtaaattctt tgatagttgc catgtacaag ttccttaatt tttcaaagcg atagttcttc
     1321 gccatgtatg gtaacaagct tttcaatcct gaagcgtttg gtccctgcag gaataataac
     1381 cgatacttca tcgccaacag tttttttatg taatgccctg cctataggcg atgacaatga
     1441 tatcttgcct tccgaagcat caacctcatc cggggtaacg atagtaaacc gggcaacctc
     1501 atcattatcc agattgcgaa cagtcacaac agagccaaag tcaacctttc cctgttcaat
     1561 agtggatatg tcgatgctat caataaggct gagctgtttg cttagatatg aaatctgtgc
     1621 ctgcacaaac gattggcgtt cacgggcagc atggtactca gcattttcct tcaaatcccc
     1681 ttttgatcgt gcttcggcaa tgcgtttagg caattctatc tttaattcat gctgtaattt
     1741 ttcaatctct tccttcactt gttttttctt atctgctatg tttactgcca tcacgtattc
     1801 ctccgaaaat agaaatagta ctgcgccatt atgaaaaagc gttagcgacc aaaaaaccta
     1861 tccttttaac agcgagcttc ttcatttcat tcagaatgac ataccagcat taccgtcatt
     1921 ctgagggaca cagccccgaa gcatctattc gtttataaag gagtttcttc gctatcgctc
     1981 agaataacga taactctaca ttttcatccc gtagttggta agcttaacaa ccatgggtaa
     2041 cttatcacaa aactacagtt atgcgatagt tactctatgt atcagtaact atcacccagc
     2101 ataacgtcaa agtgatactg ttgcttcttt aataaaagat gtctacaatt tttttatact
     2161 gccttttcaa cctttaccat taccgggttt accatatttt ttgctgcaat gtattgtgac
     2221 actatctgta taaaaacaaa tgataacgct aaaaagataa tccccataac tatcgccgaa
     2281 agctccctgt cgttaaaaaa tggtaacggg taaagcgtcc ctgcaacaat accagcaata
     2341 agaagcacaa tgggtatgcc gtacaatacc cctgaagaaa gaacaactgc ccctggtgga
     2401 aaaacaaagt ataccctgtc gccaaccttt gcatcaagag tattttgtat ccagatatcc
     2461 cttcccttct ggcgcatcat acactggctt gaacatgtag cacaaagcga agcaggatca
     2521 gccgccacac aaacaaggtt gcctcgcacc tcttttacca taccacattc agtatccatc
     2581 caccggtcct tcttaattat gatatataaa tgatatatac cacaaaaaca ggcatttatg
     2641 taaaataatt atttgttact cagtaataac taccatgtga caatacaaca actgattaaa
     2701 tacaaaccaa aaaatgaata aatgaaataa atgaaataaa tgaacaggag atatgacata
     2761 taaaattacc agcgtgcttt tacccctgta aaaaaatagc atgtatcaaa gtgctcctca
     2821 acattccatg cgcaccccac tgttgcaact atacgtccat acacaatatc tactgcaaac
     2881 ccataactgg cggtgttatc ccatgctgca tattcatcct tatagatgcc tgccgaacca
     2941 aatatcttac aatacaaaag ctcgtacaaa gcttcatata ccaattgcat tccgactata
     3001 tgacgggaat gagttgaggt atagcggtca gaatatccta acaggtaact atcaaacaca
     3061 tactcggcat atatccgttc actggatgtt gtatggaagc tatatatgtt tggaatacag
     3121 tagaaacggc tggttatttg aatagggact gagccagcaa tacgggaggt tataaaagct
     3181 tcattggcat acgctgctat aaacgacagg gtaaccatgg atttttcata tacaatagaa
     3241 tgcctttgat cagttgccgt aatacgcaac tccgctccag catcatgcga tacatcgttc
     3301 tcaagttctg acatgctgta atatgctgcc acgccataga taaacgctga aacatcatac
     3361 atgcctgcct ccatgatggt acccaatccc agatacgccc tgcattgtgt cagggtaaag
     3421 ctgcgtgaat agcgctccca cacgccatat tcctcctgag catcaatacc agtatgccag
     3481 gcagcttttg agcgatagtt catgtaatca aatccgccct gttttaaggt tatacgctgg
     3541 tcatcaaaag ccatctgccc ctttactgaa caatacgcat cctgcatggg catggtcacc
     3601 gtgatggcag gctttatacc atagatgggc agcattgcaa ttgtaaatga atagataaaa
     3661 tgctctggtt tggcctcaat aatgaggctg acatcactgc cattgttgta attgacaaca
     3721 ttgacattga tggaccgaag catatatttt tgctttaact gtgcaagaag gttatagact
     3781 gtatgtttgt tgtacactga gcccagcaat gctgttacat cctgtgttag agcatcctgt
     3841 atataggcat ccgtatggac ctgaatatca gcaatatgcg actcaacaat atacagagta
     3901 catacatcat cacgaagttc atagtgtgct acataacttg tggtatatcc ctgactgtgg
     3961 agcctgttgg taataactat cgcccagtat tccgcttctt tttgattatt gacaccctga
     4021 ttgatgatgt cctgaatatc aacattaacc gttaagccct caacagctat tgttgccatg
     4081 caatcagcag caaataaaag aaaaaagaaa agaaaaaaaa gaacaccact acactggcgc
     4141 acactatcct cacatcgttt tagctctgac accttttgga agctgtaagg taaagatatc
     4201 agtggatacg ttaccattga gaataatatt ataatagcgc gttacattgc gtgttgtccc
     4261 ggtatgcgta cgtgctataa cctctacggt agccgggaac catataccct gtatcaaggt
     4321 gtaatcgctg tacaattccc tcataacctc aatagactgt gcagtatagg taatcttttt
     4381 taccgggtag cctttatctt tatcaatggt taattctaag gtaacaccgg tttttgaatc
     4441 aaacaatgta tagcgatagc cgctattaaa aaaaccatac cataccttct ttttactttt
     4501 aacaacaatc ctttcgttta cgatatcaca ttgcgcatac ggatggacag caggaacatt
     4561 cccctgtgac attgtccata gctctttagt ttcaggataa taccacaaga gcttccctcc
     4621 attgacaata actatctgcc gaaaaggctt actgtagtca atgcgcatgc aatcgttata
     4681 tgcccggtat cgcccttcat agcgctgagt ttcttttcct gtttcggcaa taacctgcac
     4741 aatggatgca tcaatgcttt gtacattgaa attatgaaaa agtttttgaa gttcatcggt
     4801 atcggtttgt cccgaaagca ataataaaac taccaataca agatttttca ttgcattatt
     4861 ctcatcacca ttttattgtt gtaccatcat tgctctgaca ctgtaaagta ctctcatgta
     4921 attgtcaagg taaagctatg cgatatctca gtatactcat tattttattt ttaacctgca
     4981 ccggtgcatt tgcacagatc aacactgcgg cgcagccatt gcaataccct ggtgctacaa
     5041 tcagcaccca aacaccctat tttatatggt gtgatatcta taacaccgcc gccaaaccat
     5101 ttgtagttaa gctaacactc actgacagca ggggtcagag caatgaattt accctgactc
     5161 ccctgacagt ggatagcata tattgcgttg ctaccatacc tgttgttctg accccggaca
     5221 cctacacata caccatacac ctgctttcaa acaacaaacc cgtcaatagc cgatattatc
     5281 atcataaacg ataccctgta aaaggaagct ttaccataga tgtatcccgg cacaatgctg
     5341 ttgataccct caatgcaaat gagcttgtgt attatttaag ccagtcgcgt tctaatatgc
     5401 tgcacaacgg ctacaatgcc atgttctttt ctgccagcgg tatcatttca acatgtgcag
     5461 gcgttgcggt atactattgg accaattttg ggattgttac aaccattatc tcagcaataa
     5521 ccataacctc aggggtaata ggcatatcag caggaatcta ttacggtatg cagtactatc
     5581 acaacgaggc tacaatcaac aaagtcgtta ctcatgatac ggtgccacag ccacaccttg
     5641 cccctgacgc tggcaagcaa tagtggggtc agagcatcat aaaaagattg aaaaatgccc
     5701 aaaaacactg tacactatag taataattat caatgcatca atcaacatcg caaccatggg
     5761 gtcagagcct gatacggatt cactgtggtg ctaaatagta tcctctataa agttgtaata
     5821 atgcaatggt cctgaaaaaa ttaacacaaa tctatgcctc tactgatgag tgcaaaccac
     5881 tattagcatc ccggggagct ttttctatag aaggtattca cccttcaacc ttcccgttta
     5941 ttgttgcaac agcgtttaac catgcaccat cgcagataat cgcagttaca gcagattacc
     6001 acgcaatgaa tcagtttata gcctgcctat cagcatttac cagcgaagaa catatatttc
     6061 cctatcctcc ccttgaggtt ttaccctatg agatggttaa tccttcatca gctatcgagc
     6121 gcgaccgcat cacagcgctg tatcagcttt tatcgggtaa tcccgtcatt gttgttgcaa
     6181 ctgttgaatc tctgctcaga gccatccctc ataaagaatt ttttttaaaa aaaggcatca
     6241 ccatccataa gggcgaggaa tatccctttg aggttatcct tgaaacgctt gccagctacg
     6301 gctatgagcg tgaaacccgt gttgaccgtt ttgggcaatt ttcggtaaag ggaggtatca
     6361 tcgatatatt cctgcccaat tgcaccaatc cggtgcgctg tgattttttt ggggatacct
     6421 gcgaatccat ccgctacttt gaccctgtga cgcaggtttc gcttacggaa actgatgcaa
     6481 ttaccattta tccgcgccgt gaacttttgt tgtccccatc acaaaaagaa caattaaaaa
     6541 aagccttaca acctgtgggc gacagttccc ttcaaaagca acttgatagc atggaaaaac
     6601 aatccatacc gggtatttac gacctgttcc ctatcattat tccgggcacc accatactgt
     6661 caattgcaac acaaccaacc gtttttttgt gtgacccatc acagctcaaa gcaaaacttg
     6721 cagaattgga acgaacatac catcagctct acatgcacaa aaaggatatg gcagtgttcc
     6781 cccatgattc acaaattttg ctgggcgata gttacccgga aattgaaaag caggcggtac
     6841 gtcttcaacc gtttatagct gactcatccc tggtatggca gttaaaaagc ataccatcct
     6901 ttcagggacg cattgccgat gttcgcagtg aatgttcaaa gcgcattgat gaaggatggc
     6961 acatcattgt cagcacatcg tttgaaggac aggcaagaag attatacgac ctcttcggcg
     7021 agtttcatcc tgaagatacc tttgatgcat ttaacccgga taagcaattt actattgtca
     7081 tttcaaatct tcatgaaggt tttgaaatat cggcaataaa aaccttgatt attaccgacc
     7141 atgaaatttt tgggaaatca taccggaaga aaaaacattt taaacaaaaa tattcccgac
     7201 caatcgatac atttttagaa ctatcgccgg gagattacgt tgttcatatt aaccatggca
     7261 taggtgtttt taaggcaatt gaacgcatga gcgcaggtgg cgttgaacgt gattttattg
     7321 ttattgaata tgcagatggc gataagctct atgtatcgct tgatcagatc aatatggttc
     7381 agcgctacgt ggggcttgat ggcaggcaac cgcgcattga tgcactgggg aaaaaatcag
     7441 catggaacag gataaagcag cgtgtgcagg aatcagtcga ggagatagca cacgacctgc
     7501 taaaaattta ttcacagcgt aaagcgctga aaggctatca atttccgcct gataccatat
     7561 ggcaggagga gtttgaatca aagtttgaat atgaagaaac gccggatcag ataactgcca
     7621 ttgaagatgt caaggatgac atggaaaaat cacagcctat ggacagattg gtttgcggtg
     7681 acgttggatt tggaaaaaca gaagtggcaa tacgagcagc atttaaggca gttatggccg
     7741 ggaaacaggt agcaatactt gttcctacaa cagttttagc aatgcagcac tataatacct
     7801 ttatcaaacg ttttgctgat tatccggtaa ctatcgacat gctatcacgc tttaaaacgc
     7861 aacagcagat aaaatccata aaggaaaaaa ttaaaaccgg tacagtagat attgttattg
     7921 gaacacatgc tctgcttgca aaggatgtaa gcttcaaaaa tttagggctt gttgtcattg
     7981 acgaagaaca gcactttggg gttaaacata aggagtatct aaaaaaattg cggctgcttg
     8041 ttgatgtcat tacgctttcg gctactccca ttccgcgtac cctgcatatg gcattggctg
     8101 gcatacggga tttaacaatt attgcaacac cgccggaaag caggcagtca atcgaaacac
     8161 tggttattga agacaatcct gatatagttc gcatggccat acaaaacgag atacaacgca
     8221 aagggcaggt attttttgtt cataatcgca tcagtaccat ctatgagcag gcacagtacc
     8281 tggaaaagct ggtccccgag gcaacgttct gtgtggcaca tggcaggatg cacgagcatg
     8341 agcttgagga ggtcatggta gactttatta acggcagata cgatgttctg gtttccacat
     8401 ccatcattga atcagggctt gatatgccca atgtaaacac catcatcatc aaccgtgccg
     8461 ataccatggg actatcgcag ctatatcagt taaaagggag ggttggcaga tctgacaggc
     8521 aggcgtatgc atacctattt tatccaaaaa atgcacccat aacagaggat gctcaaaagc
     8581 gattgcaggt catagctgaa tataccgaca ttggcagcgg ctttaaaata gccatgaaag
     8641 atctggagat acgaggctcg ggcaatatcc ttggaagaga gcaatcgggc aatatcatgg
     8701 atgttggatt tgatctgtac tgccagcttt tagaagatac tgtacgcaac cttaaaggtg
     8761 aaaagcccgt tcatatattc agaactcctg tatatgttaa aactgacatc tttatacctg
     8821 acagctatat tgctgatgaa aaacagaaga ttgaatttta caagcggtat gaagcatgcg
     8881 aaacgcttga tgaactcaac aaattaacac gtgagatgat cgacaggttt ggacagcctc
     8941 ccaaagaggt ggtatcgctt atagctatgg aagaggtgcg tacccttgct tcccagcttt
     9001 atattgatga aattattgaa gatacaaagc tcataaaaat gcgcattacc agagaatcaa
     9061 aattagatgt taaaaagctg gttaccctga ttcagaagga taagcgtata tccattgatc
     9121 gttatgataa agaggtcgtt aatgttgtta cagggataat acctcctgaa aaaaaacttg
     9181 aggaaataaa aaaattgttg caacaattat catagtatag taactatctt acaattaaaa
     9241 attgtgttga aaggcaaccc tcccattgcg gaggtatatt tttaatttta tataaaacat
     9301 agaaggatgg atactatgaa aacaaagttt ttgtattgtg caattgcaat tatggttctg
     9361 gctatggtta tttcatgcgg caaccaatac ggcggcaaat ggattgcaaa gattgatgat
     9421 gaaacgataa ccgacaatga actcaatgca tactattatg cacagatgaa aagcatctac
     9481 aatcttccta aagaagagat agataaattg gctcaggatc ctgctcagct tgagcgcaac
     9541 cctattctta ataaaaacaa ctttcttgag caaatgatac aacagcgcct tgtgtataaa
     9601 aaagccattg atgatggcat attaaaaaat gaagaattgc agactctttt agatatctcc
     9661 aaggaagggc ttgtagtgca atactatatc cgcgaaaagt ttaaaaatga tatatccatt
     9721 gccccgcagg aggttgagat gatatataac cagcagcgcg cccgttttaa aggggtacca
     9781 attgaccagg ctgaaatgta cataaagcag cagctttttc agcaaaagct caatatgaag
     9841 atcaaagagc tggttgatac attgcgtgat gaaaagaaga tagagaagaa catggattta
     9901 ctgcgcaaag aattgaatac acaatcaccg gtgcaacaag cgcctcagca ggagcatgta
     9961 ccacagcctc aacagcaaat gcaaaatcct cctgcaaaat agattgttat aattgcactc
    10021 attatagtgc tttgttattg taactgctga gcgaatgtgt gatatgcagg tagcatgata
    10081 tacgaattat gtgaattata agaagtataa agcacagttt ggctttttat tgcaaggtaa
    10141 cagtaaactg tgctttatta accatcttat cattgatcat tatgagctcg gttaatgatg
    10201 aggcttttgt ttcaaaataa aagaagtttg gctttggctg caaaatatta tttacttttt
    10261 tatattcacc ttcacgttca tcaagtaccc atttttcaac cactataaga tgcatattgg
    10321 atgatagttt agcctcaaaa tactttttct gtgattcaag cccaatggtt gtcctgccag
    10381 cgtctatctt atcaataaaa attttatagt agcaccgttt atcttcatcc ggagtggtaa
    10441 ttcctgtatc ataagcaata aacctgaaat tgacattcac ctcggttgga ctgatgattg
    10501 gctctatagg tggttcatat tttggcaccg ttttacatcc tgaagcaata aataacacca
    10561 aaaaaaataa aatgagcatc cttcttttgt tcatggtcta tcctttattt caatagtata
    10621 tagtccgtca tacatttcat tcaatggttt aacaatgcag tatcctttca tatcaactat
    10681 ctcatgcaat gtatttgaac tacctgcttt cacagtaatt gattgtatta tatatcccaa
    10741 tgaatcagta acagaaaatg atataggtat ggtatcaggc gctttaatgg aaaaatattt
    10801 tttttgacgc cctttatagt gtaaaacata aaaatcaata tcgtgttttt ttgaaatata
    10861 tccatttatc aacgttccct gcacattgtt agcctgttct tttgtatcat taggttcact
    10921 atcaacaaga tttcccattt gaacaggaac tatccgcaaa gcgtagctat tatccatgtc
    10981 aacctgtccg cgcttagccc atactttaac ataaaaaggt gcattcagct ttacattggg
    11041 atatacttcc ttatcacctg tcccgttatt atttatagaa gttatttctt cacctttttt
    11101 agtataaact gcagatacta catcaagagt cattggcgga tttatctcga tacggtaaaa
    11161 tacatcattg gaactatcgc ccatatactg ataatagtca atgtcattgt caggataaat
    11221 cctgccataa ataatttcgg taaccatggg agttgccgat tccatgatat tgtttggttc
    11281 tatctcggtt ttattatcat aatttcgagt tgtacaaata aggctgtagg gaacatcgtt
    11341 gttggcatta taatttttag ctgcaaccat aatgtagtaa ttccctgc
//
LOCUS       DAOSOX010000073        11382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5681_length_11382_cov_8.078132, whole genome shotgun sequence.
ACCESSION   DAOSOX010000073 DAOSOX010000000
VERSION     DAOSOX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5681_length_11382_cov_8.078132"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLX22_06705"
     CDS             complement(<1..402)
                     /locus_tag="PLX22_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19626.1"
                     /translation="MFDIYGSYEYNPSIFDNMQMEINDIDDDISSDIENLLANRFDSL
                     YLKDIYKKNNICIDIIYPRVQYGHHRVLLKSPDYIRFLLSLYPFSSDLENLQKIVLRP
                     RHIILGNVELMALYLQQKKILVVYLHHPHFYS"
     gene            complement(435..1415)
                     /locus_tag="PLX22_06710"
     CDS             complement(435..1415)
                     /locus_tag="PLX22_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19627.1"
                     /translation="MKDIIIFTSDEEKLVEHIVQAIESKYQTEYILIEKHKEALRSLA
                     TSISLYPSLLDSQRLQNQKRTVQSLLDTLCSRSMPDMILHIPTKAILGRAFTIAKINF
                     FIMLWYIARDKDEYTAFVEALLLCIASNVFMLTAEEVYTSIIEDDALDKIIRHNAVYL
                     LARTWEHRLDYGVAEFAPILLNLWKARERLIPNFGTMMGFSELCMLSEHTSSLWLDFL
                     QRDAIMEDEVYALEEFIFDLTYEEIAFVRDYLEKNSKTTVMREELPSIMGKTHIPEYS
                     GQDPRELYRSFRDRKINSRFRARSKLNGPKKTLEEYLMCFLLSSKTVVEY"
     assembly_gap    1503..1602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2088..2912
                     /gene="ccsA"
                     /locus_tag="PLX22_06715"
     CDS             2088..2912
                     /gene="ccsA"
                     /locus_tag="PLX22_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT19628.1"
                     /translation="MTLLVYLTFFTFIITSVYSILSIHRDASSYIRVTKVAIIVAIIL
                     LFFQGCFLYKTIAHAYKINIFTFFYLIVVFLSLLLLFMSISRWKDDFRALSAIITPLI
                     TVIYGISLLFYGSNRFMAIEPNHNLMLIHIVLSLLGEVLFFIAFASAVLYLVVSWQLQ
                     KKSSMKFIYRFPSLGVLNRLTNFSLAHSLAFFSAGIAIGIIMLFEVYHNISLGSPKEI
                     LMYAVWCILLIVWIIAHSKITSNFYVSIITIVAFVIVIVLFIATNVVITTGFHSFR"
     gene            2918..4204
                     /gene="hemA"
                     /locus_tag="PLX22_06720"
     CDS             2918..4204
                     /gene="hemA"
                     /locus_tag="PLX22_06720"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOT19629.1"
                     /translation="MAECTQQIGMVGMSHKTASVETRECFAFDTAVLESFVDNAKKNN
                     IDEMVYIATCNRMELYFVSSQIEEAMQSVVQLLEQFAGLKREQFEPVLYKKFHKDVIR
                     HLLCVASSLDSMVIGENEIVGQVKNWYRWAAVHKTTGPILNKLFHQAFRTAKRIRTQT
                     EIARNPLSIAFIAVELARKIFDDLASSSVLLVGAGEMSELILKYLTKFKIGTVILANR
                     SLHNAQRIATEINRDATIIPLSEIIDVIPEVDIIITSTGSHEYILTRDMITEHIKKRS
                     QPLFLIDIAVPRNIEPAVSTIHDVFLYNIDNLKSIADENLKNRIHETKLAMELIDADA
                     EDFLHWYQSLEIVPLIVSIQETFSTIRNEELQKYRRRKLKHLSDDDFAIVEELTNQIM
                     SKTLHIPISYLKDLSDNYHKKESKEKTKLLQEMFKR"
     gene            4201..5259
                     /locus_tag="PLX22_06725"
     CDS             4201..5259
                     /locus_tag="PLX22_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19630.1"
                     /translation="MNKPRLIFWELTKRCNLRCVHCRAESYDKEFEHELSLQDCKNIL
                     DSIAAYYKPIMVLTGGEPLYRKDLFDIAGYANGKGLPIALATNATLIDREIASAIKKS
                     GFERVSISIDGPDALTHDSFRGIPGSFDKALQGAQLLHEAGVPFQFNTTITRRNVTSI
                     EKILQLAKTMHAVALHIFMLVPVGCGVEIAQTDMLSKEQYEEVLRWFYHTTQEAGIEF
                     KATCAPHYYRIIRQEAKKENRKLTFETDGLAAVTRGCLAGSGVCFISHRGDVQPCGYL
                     PLVAGNCLLTPFNELWESSELFLKLRDLSNLEGKCGVCEYKAFCAGCRARAYYASGNY
                     MDEEPFCIYQPKRATHAG"
     gene            5249..6226
                     /locus_tag="PLX22_06730"
     CDS             5249..6226
                     /locus_tag="PLX22_06730"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19631.1"
                     /translation="MLDEKKLISQIQIAVPVTKRPYRVLADTLGTTEYEVINTVQKLK
                     QDGLIRSIAGIFDAKKLGYTSNLVAFAIPEPAIAHGVAIINALPGVSHNYLRDGAYNI
                     WFTLALPEETNFIETVQHLAEQVNAVTFNIFQAVLTVKLSARFFEDDSIHDNNTQYAV
                     TGKHNETIPVDDTVKNTVKVLQCDLPCSEEPFDQLIAQSNIPMDVDLFIATANRLLEN
                     GMMRSYRAVVKHHAMGFVANAMTVWKADGDKRNSMLEQFANENAISHLYVREAYPNPW
                     DYPVFAMVHAKSEDELDMIIKKLYSIAKTDYMSYRSLQEFKKERVRYFE"
     gene            6323..7591
                     /locus_tag="PLX22_06735"
     CDS             6323..7591
                     /locus_tag="PLX22_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19632.1"
                     /translation="MIDIGKLYCGGSSTGDGLRYGTEASVDAHGNLPHTRVAMADQRK
                     PIVVWNVTRTCNLNCVHCYTDSHNKKYGELSTDEGLTLIDDLATFGIPSLLFSGGEPL
                     MRRDIFRLITKAADKNIRPVLSTNGTLIDRDKARAIKDAGIVYVGISLDGMEDVNDTF
                     RGMDGAFKKAMKGFENCIAVGQRVGLRLTLTKRNYADLHNIFDFIEKEGINRACFYHL
                     VYSGRGGSLFADDLSYSESRQAMDTIIERTDDFFRRGLDINILTVDNHVDGVYLYLKL
                     LQKDEQQAASIAQLLTWNGGGAYSTGVGIANIDFEGNVHPDQFWQDYSFGNILTRSFA
                     DIWMDESNPLMKGLKHKADYIKGRCRLCKFKPMCNGSMRVRAYRVFGDPWAPDPQCYL
                     TDEEIGLTHDTIIALKDAGEYYTMPEELAQ"
     gene            7614..8243
                     /locus_tag="PLX22_06740"
     CDS             7614..8243
                     /locus_tag="PLX22_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOT19633.1"
                     /translation="MYRIKGKHVVVIGAGEVAYRKVKDLIAAGAIVTVIAPHIGKDLS
                     ALANDHSVTIIQRLYQKGDCKGAALVFSATNNTEVNQQVFYEASELNIPINAVDDPDN
                     SSFYVPSFFTRGKLLVAVSTGGASPAMAAKVRRLLEQAIPQDIEQALDALTAIRKLLK
                     ENFPHLTSNERSTVLKTIANDEHLIARTIASHNNNTLHELLNELLMNKK"
     gene            8316..8642
                     /locus_tag="PLX22_06745"
     CDS             8316..8642
                     /locus_tag="PLX22_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19634.1"
                     /translation="MDIKHEIKGNVLIVSIEGELTSPQALDVDKKIINEIEKEKKERI
                     IIDLSKLDFIDSTGLGVLVSIRLYCKKNKILFATVGVKEKVKRTFTVTEMTSFLNIHD
                     SMDKLL"
     gene            complement(8710..9705)
                     /locus_tag="PLX22_06750"
     CDS             complement(8710..9705)
                     /locus_tag="PLX22_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT19635.1"
                     /translation="MMSEYIAGVDIGGTKIIATIADVTGIKSRVYQQTQLHGDNFTIP
                     KQVYALIQLAAENAQIQYDEIKSVGVSTCSPFLRNGVYKEIVAPNLCGGLTDSGRLPN
                     DWQSIALEEFLCNIYPHVIVENDCVASVVAEKLFGAGKFIDNLVYVTWSTGIGAGIYA
                     DGKLIRGKNGNAPHFGHIFIAEDGPQCGCGNYGDLEALASGSAIARDYHGSSAKIVFD
                     DYRKGNEKAIAIIKRAARNFARGLASLNAMVDTEYISLGGSVMNDSDILLPMIREEFY
                     QSFKVLSHNVIIEKSALFQYIADIAALALVMPASWIEQWRTYSPWLQSPGMIKLN"
     gene            complement(9702..10094)
                     /gene="queF"
                     /locus_tag="PLX22_06755"
     CDS             complement(9702..10094)
                     /gene="queF"
                     /locus_tag="PLX22_06755"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOT19636.1"
                     /translation="MTDYSKANDAVIEKVQGYIFDTIPARGHETIVRWEYPEFQSLCP
                     VSGRHDQGTVIISYKPAKLLLESKSVRNYLGAWRNKSIWQEYVTDVIAQDLFDAAKPQ
                     WIEVSIQWAPRGGIFATTTSVKGNKKKL"
     gene            10408..>11382
                     /locus_tag="PLX22_06760"
     CDS             10408..>11382
                     /locus_tag="PLX22_06760"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOT19637.1"
                     /translation="MNKYIAQVAVVTLLVFFTYSAFAQENGLTSPPSSDIPVSDIPDN
                     NTAVTNTPDATVNNSTSSWNPFTKGNGPTTPTLAMFIAVPVITTVYGMETWDWGEQKN
                     PELADEGFFGKNTDSGGADKAGHAYAHYLSFRVFHAYYDWSENGRNTKWFYSIATATF
                     LGVFIEVGDAFTGKYGFSYEDVVADVAGIGLGILLEYSPTLDSLIGFSWQYWPTEGYT
                     DRWGANPLHMTSDYSGAKYVLNFRLAGLQNLGLRLPNFLRYIQLDFGYFTRNYVRWDK
                     TDDDPYRSLYFGISLNFMEVVKDFFDNPESRTCKILQQPFKYYHIPAGY"
BASE COUNT         3501 a         2177 c         2194 g         3410 t
ORIGIN      
        1 tgaatagaaa tgaggatgat gcaggtatac taccagtatt tttttttgtt gcagatacaa
       61 agccatgagc tcaacattgc ccaatataat atgccgtggg cgcaaaacaa tcttttgcag
      121 gttttcaaga tcactggaaa atggatataa tgacaaaaga aaccgtatat aatccggtga
      181 ttttaacaac accctgtgat gaccatactg aacgcggggg taaataatat ctatacagat
      241 attatttttt ttatagatat ctttaaggta taatgaatca aaccggtttg ccagcagatt
      301 ttcaatatca cttgatatat catcatcaat atcatttatc tccatctgca tattatcaaa
      361 tatggatggg ttatattcat atgaaccata aatatcaaac atacctttcc ctgtataata
      421 ctccttgcta ttgcttaata ttcaaccact gttttagaag ataataaaaa gcacatgaga
      481 tattcttcaa gcgttttttt aggaccattt aattttgatc gtgctctgaa cctggaatta
      541 atcttgcgat ctctgaatga acggtaaagc tcgcgggggt cctgcccact atattcaggt
      601 atatgtgttt tccccatgat tgatggcaat tcttctcgca ttacagtagt tttactattc
      661 ttttcaagat aatctcgcac aaaagctatc tcttcataag taaggtcaaa tataaattct
      721 tcaagtgcat atacctcatc ttccataata gcatcacgct gtaaaaaatc aagccataat
      781 gacgaggtgt gttctgaaag catacataat tcagaaaagc ccatcatagt accaaaattt
      841 gggataaggc gttcacgtgc tttccataaa tttaacaaaa tgggcgcaaa ttcggcaact
      901 ccataatcaa gcctgtgctc ccatgtacgc gctaaaagat acactgcatt gtgtcgtatg
      961 attttatcaa gtgcatcatc ttcaattata gaagtgtaca cctcctcggc tgtgagcata
     1021 aatacattgg aagctataca taataatagc gcttctacaa atgctgtata ttcatcctta
     1081 tcacgcgcaa tgtaccatag catgataaaa aaattgatct tggctatggt gaacgccctc
     1141 cccagtatag ctttggttgg tatatgtaaa atcatatcag gcatggaacg tgaacacagg
     1201 gtatcaagca aactttgcac ggtgcgtttt tgattttgaa gacgttgcga atcaaggagc
     1261 gaaggatata acgaaattga tgttgctaaa ctgcgcaatg cctctttatg tttttcaatg
     1321 agaatatatt cagtttgata tttactttct attgcctgca caatatgttc aacaagcttt
     1381 tcctcatcag atgtaaatat tatgatgtct ttcattggca tagatatcac ccattaacat
     1441 taataagatt agacttcctg tggataattt tgtcaatatt taatttgaat aaaatccatt
     1501 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattagact tcctgtggat
     1621 aattttgtca atatttaatt tgaataaaat ccattgcggc acatggttag ttagtataaa
     1681 gcattgtatg aataacatag gtatagtaaa taaagaagaa atgtaaaaaa aatgacgacc
     1741 cctcttgtga ggaatcgtca atatcattag cggattatgt tggaaggtaa ctactcaatg
     1801 agtagcctca ctaataatag taaataaaat agataaaaga gtcaaatata tttttgccaa
     1861 tttagtaaaa aaatgacata aaattatact atcttataac aggacaaagc actttgcata
     1921 ccatgtaatg atgcaataac atcctaatat gacagatagt ttgtatagat gattactatt
     1981 gcatagtgtt caggaatgcc cattattgct tgacactatc gtgcaatgac aatatttatg
     2041 aattgttata aaaacgatat atattgaaag taacatagtg aggctgtatg acgctactgg
     2101 tttacctgac attttttaca tttatcatta cttctgttta tagtatcctt tcaatacaca
     2161 gggatgcttc gtcatacata cgagtaacaa aagttgccat tatcgttgct ataattcttt
     2221 tatttttcca gggttgtttt ttatataaaa ctatcgctca cgcttataaa attaatattt
     2281 ttaccttctt ttatcttatt gttgtttttt tatctctatt gcttcttttt atgagcattt
     2341 caaggtggaa agacgatttt agagcactaa gcgcaattat tacgcccctc attaccgtta
     2401 tttatggcat ttcgttactg ttttatggtt caaacaggtt tatggctatt gaacccaatc
     2461 ataatctgat gctcatccat attgttttat cattgcttgg cgaggtttta ttctttatag
     2521 catttgcttc tgctgtttta tatttagtag tatcatggca attgcagaaa aaatcatcca
     2581 tgaaatttat ttatcgtttt ccaagccttg gtgtactcaa caggcttacc aatttttcat
     2641 tagcacattc gctggccttt ttcagcgcgg ggatagcaat agggattatt atgctttttg
     2701 aagtatacca taatatttca ttgggcagcc cgaaagagat tttgatgtat gccgtgtggt
     2761 gtatattatt aattgtatgg attatagcac attctaaaat taccagtaac ttttatgtga
     2821 gcataataac aatagttgca tttgtgattg ttattgtttt atttattgct accaatgttg
     2881 tcataacgac aggatttcac agtttcaggt aagcagcatg gcagaatgta cacaacaaat
     2941 tggcatggtt ggcatgagcc ataagaccgc atcggttgag acaagggagt gttttgcatt
     3001 tgatacagcc gtacttgaat catttgttga caatgctaaa aaaaataata ttgatgagat
     3061 ggtatatatt gccacatgca atcgtatgga gctatatttt gtttcttcac agattgaaga
     3121 ggcaatgcaa tcagttgtac aacttttaga gcaatttgct ggtttaaaac gtgagcagtt
     3181 tgaaccagtg ctttacaaaa aatttcataa ggacgtcatc cgacacttat tgtgcgttgc
     3241 ttcttcattg gactcaatgg tcattggcga aaacgagata gttggacagg ttaaaaattg
     3301 gtatcgctgg gctgctgttc ataaaacaac aggtcctatc ttaaataagc tgtttcacca
     3361 ggctttccga acagcaaaac gtattcgcac acaaactgag attgcccgca atcccctatc
     3421 aattgcattt attgctgttg agctggcacg aaaaatattt gatgaccttg catcaagctc
     3481 ggtgctttta gttggcgcag gtgagatgag tgagcttata ttaaaatatt taaccaagtt
     3541 taagattgga actgttattt tagccaacag gtcactgcac aatgcccagc gtatagccac
     3601 cgaaataaat cgtgatgcta ccattatccc gttatcagaa attattgatg tgataccaga
     3661 agttgatata ataataacct caacaggatc acatgaatat attttaacca gagatatgat
     3721 tacagagcat ataaaaaaac gtagccagcc actattttta attgatatcg ccgttccacg
     3781 caatattgag ccggcggtga gcaccattca tgatgtcttt ttgtacaata ttgataatct
     3841 aaaatccata gctgatgaaa atttaaaaaa tagaatacat gaaacaaaac ttgccatgga
     3901 acttattgat gctgatgcag aggatttttt gcactggtac cagagtctgg agatagttcc
     3961 gttgattgtg tcaattcagg aaaccttttc aaccatcagg aatgaagagc tgcagaaata
     4021 tcgcaggcga aaattaaaac atctttcaga tgatgatttt gctattgttg aagagctgac
     4081 caatcaaatt atgtccaaga cattgcacat acctatttct tatttaaaag atttatcaga
     4141 taactatcac aaaaaagaat caaaggaaaa aacaaaattg ctgcaggaga tgtttaaacg
     4201 atgaataaac ctcgcctgat cttctgggaa ttgaccaagc ggtgcaacct gcgttgtgtt
     4261 cactgcagag ccgaaagcta tgataaagaa tttgaacatg aactatcgct tcaggattgt
     4321 aaaaatattt tagatagcat tgcagcatat tataaaccta ttatggtgtt gaccggtggt
     4381 gagcccctgt accgtaaaga cctttttgat atagcaggct atgcgaatgg caagggatta
     4441 cccattgcat tagccaccaa tgctacattg attgacagag aaatagcttc agctataaag
     4501 aaaagcggat ttgagcgtgt tagtatttcc attgatggcc ctgatgcact aacccatgac
     4561 tcatttagag gcatccccgg ttcatttgat aaagcattac agggagcaca attgttacac
     4621 gaagcaggtg taccgttcca gtttaatact acaattacca ggcgcaatgt tacctccatt
     4681 gaaaaaatat tacaactggc aaaaacgatg catgcggttg cccttcatat atttatgctt
     4741 gtaccggttg gttgcggagt tgagattgca caaactgata tgctatcgaa agagcagtat
     4801 gaagaagtat tgcgctggtt ttaccataca acacaagaag ccggcataga gttcaaggct
     4861 acctgtgcac ctcactatta caggattata cgccaggaag ctaaaaaaga aaatagaaaa
     4921 ctaacctttg aaaccgacgg gcttgctgct gtaacccgtg ggtgccttgc tggcagtggc
     4981 gtatgtttta tttcacaccg gggtgatgtt caaccatgcg gctatcttcc acttgtagca
     5041 ggcaattgct tactcactcc ttttaatgaa ctatgggaat catcagaact ttttttaaag
     5101 ctcagagatt tatcaaatct tgaaggaaaa tgcggcgtat gcgaatataa agcattttgt
     5161 gcaggatgcc gtgcacgcgc ctactatgcc tccggcaact atatggatga agaacctttc
     5221 tgcatatacc aaccaaaaag ggcaacccat gctggatgaa aaaaaactta tttcgcaaat
     5281 acagattgca gtaccagtaa caaagcgacc ctacagagta ttggcagata ccctggggac
     5341 cactgaatat gaggttatca acacagtcca aaaattgaaa caggatgggc tgatccgcag
     5401 tattgctggt atatttgatg ctaaaaagtt aggatataca tcaaacctgg tggcatttgc
     5461 aatccctgaa cctgctattg cccacggtgt tgctattatc aacgcgctgc ccggtgtcag
     5521 ccacaactac ctgcgggatg gtgcatataa tatatggttt acccttgcac tgccagaaga
     5581 aactaatttt attgaaacag tgcaacatct ggcggaacag gttaacgctg taacatttaa
     5641 tatcttccag gcagtgttga cggttaagct gtctgcacgt ttttttgagg acgatagtat
     5701 ccatgacaat aatacacagt atgctgtaac cggtaaacac aatgaaacca taccagtaga
     5761 tgataccgtg aaaaatacgg tgaaggtgct ccagtgcgac ctcccatgca gcgaggagcc
     5821 atttgatcaa cttattgccc agagcaatat ccccatggat gttgacttgt ttattgcaac
     5881 agcaaaccgc cttttagaaa acgggatgat gcgatcatac cgtgctgtgg ttaaacacca
     5941 tgctatggga tttgttgcca acgcaatgac cgtatggaaa gccgatggtg ataaacgtaa
     6001 ctccatgctg gaacaatttg caaatgaaaa tgccatatcg catctgtatg tgcgtgaagc
     6061 ttatcctaac ccatgggatt atcctgtctt tgcaatggtt catgcaaaaa gtgaggatga
     6121 acttgatatg atcatcaaaa aactctattc aattgcaaaa actgattaca tgagctatcg
     6181 atcattacaa gaatttaaaa aagaacgggt acgatatttt gaataacagc gactacaaat
     6241 agtatggtaa aatggtgctt gataatacgt ttatgtaatg ttaaacaatg cctatagagg
     6301 attgtaataa tgtgaggtaa ccatgataga tatcggcaaa ttgtattgtg gcggttcatc
     6361 aacaggtgac ggactgcgtt atggcactga ggcatcggtt gacgcgcatg gcaaccttcc
     6421 tcacacgcgc gtggcaatgg cagatcagcg caaaccaatt gtagtgtgga atgttaccag
     6481 aacatgcaat ctaaactgtg tgcactgtta tacggattcg cataataaaa agtatggtga
     6541 gctttctact gatgaggggt taacgcttat tgatgatttg gcaacatttg gcattccctc
     6601 gcttttgttt tcaggtggtg agccactcat gcgcagggat atattcaggc ttataacaaa
     6661 agcagcagat aaaaacatcc gcccggtact atccaccaac ggtacattaa ttgacagaga
     6721 taaagcacgt gccataaaag atgcaggtat tgtctatgtt ggtataagcc tggacggaat
     6781 ggaggatgta aacgatacat tcagggggat ggatggcgca tttaaaaaag ccatgaaagg
     6841 ctttgaaaac tgtattgcag ttgggcagcg cgtgggatta cgattgacat taaccaaacg
     6901 caactatgcc gacctgcaca acatatttga cttcattgaa aaagaaggga ttaaccgcgc
     6961 ctgcttttac catcttgtct attcggggcg tggcggttca ctgtttgcag acgacctttc
     7021 atactctgaa tcacggcagg caatggatac catcattgag cgcactgatg attttttcag
     7081 acggggactg gacataaaca tccttacggt ggacaaccat gttgacggcg tttatctata
     7141 tttaaagcta ctgcaaaagg atgagcaaca agctgccagc atagcgcaac ttttaacatg
     7201 gaatggcggc ggagcgtatt caaccggtgt tggcattgcc aatattgatt ttgagggaaa
     7261 tgttcatccc gatcagttct ggcaggatta ctcatttggc aatattttaa cgcgaagttt
     7321 tgccgatata tggatggatg aaagcaatcc attgatgaag ggcctcaagc ataaggcaga
     7381 ttatattaaa ggcagatgcc gactgtgcaa gtttaaacca atgtgcaacg gttccatgcg
     7441 tgtgcgcgcc tatcgggtat ttggcgaccc atgggctccc gacccgcagt gctatcttac
     7501 tgatgaagag ataggattaa cgcatgatac cattattgca ttaaaggacg ctggagagta
     7561 ctatacaatg ccggaggaat tagcacaata gcaagcatat ggtgtatcca ataatgtaca
     7621 ggataaaagg caaacacgtt gtggttatag gtgccggtga ggttgcctac cgcaaggtga
     7681 aagaccttat tgctgcgggg gcgatagtta ctgtgatagc tccccatata ggcaaagatt
     7741 tatctgcact tgccaatgat cacagtgtta ccatcataca gcgcttatac cagaaaggtg
     7801 actgtaaagg tgctgcactt gttttttctg ccaccaacaa cacagaagta aaccagcagg
     7861 ttttttatga agcatctgaa ttaaatatac ccatcaatgc cgtcgatgat cccgataact
     7921 catcatttta cgtgccatcg ttttttactc gtggtaaact gcttgtagcc gtgtccaccg
     7981 gtggcgcatc tcctgcaatg gctgcgaagg tacgcaggct cttagaacaa gctatcccgc
     8041 aggatattga acaagccctt gatgcattaa ctgccatacg caaactattg aaagaaaact
     8101 ttccccatct taccagcaat gaacgaagta ctgtgttgaa aacaatagct aacgatgaac
     8161 atctgattgc acggacaatt gcatcgcata acaacaacac attgcatgaa ttgctcaatg
     8221 aattattgat gaataaaaaa taatccttga ttttactata cgttactgtt ctatgtaatt
     8281 gtatataaac attgtgtaca ggaggttatt tgttcatgga tatcaaacat gaaataaagg
     8341 gtaacgttct tattgtttct atagaaggag aattgacatc acctcaagcg cttgatgttg
     8401 ataaaaaaat aattaatgaa atcgaaaaag aaaaaaaaga acggattatc attgatttgt
     8461 caaaactgga ttttattgat tctaccggat tgggtgttct tgtatcaatt agattatatt
     8521 gtaaaaagaa taaaatttta tttgcaacag ttggcgttaa agaaaaggta aagcgtacat
     8581 ttaccgtaac cgaaatgaca tcgttcctca atattcatga ttcgatggac aaactgctgt
     8641 agcatacaca agcattaaac gcttttattt tttactgcca ttgtaaaaac aaaaagtatc
     8701 gagtaaccat caattgagct ttatcattcc tggtgactgc agccatggac tataggttcg
     8761 ccattgctca atccatgatg ctggcatgac aagggctaat gcagcgatgt cagcaatata
     8821 ctgaaagagt gcgctttttt ctataatgac attgtgggat agtaccttaa atgattgata
     8881 aaactcttca cgaatcatgg gcagtaaaat atcactatcg ttcattacac tgccgccaag
     8941 gctgatatat tctgtatcaa ccattgcatt aagcgatgcc aatccgcggg caaaattacg
     9001 agcagctcgt ttaattattg ctattgcttt ttcattgcct ttacggtaat cgtcaaagac
     9061 aatcttagct gaactgccat gataatcccg ggcaatagca ctgcccgaag ctaacgcctc
     9121 taaatcccca taatttccac atccgcattg cggaccatct tcagcaataa agatatgccc
     9181 aaaatgcggc gcattcccat ttttaccgcg tataagcttt ccatcagcat atataccggc
     9241 acctataccc gtgctccatg ttacataaac aagattatca ataaatttac ctgcaccaaa
     9301 aagcttttcg gcaaccactg atgctacaca gtcattttcc acaataacat gcggatatat
     9361 gttacataaa aattcttcaa gtgcaattga ctgccagtca ttaggtaagc gtccactgtc
     9421 tgttaaacca ccacataaat taggggcaac aatttcctta tatactccgt ttctcaaaaa
     9481 ggggctgcat gtactaacac caacagattt aatctcatca tattgtatct gtgcattctc
     9541 agccgcaagc tgtattaatg catacacctg ttttggtatg gtaaaattgt caccatgtaa
     9601 ctgtgtttgc tgatacaccc gtgattttat tccggttacg tcagcaatag ttgcaataat
     9661 ctttgttccg cctatatcaa caccagcaat atattcgctc atcatagctt tttcttgttc
     9721 ccttttacag acgttgtggt ggcaaagatg cccccgcgcg gagcccactg tatggacacc
     9781 tcaatccatt gtggctttgc cgcatcaaat aaatcctgag ctataacatc agtaacatat
     9841 tcctgccata tagatttgtt acgccatgca ccaagatagt tacgcactga tttgctttcc
     9901 agcaacaatt tagcaggttt atagctgata atcacggtgc cctgatcatg cctgcccgaa
     9961 acagggcaaa gactctggaa ctccggatat tcccacctga caatagtttc atgccctctg
    10021 gcagggatag tatcaaagat atacccctgc accttctcga taacagcatc gtttgctttt
    10081 gaataatcag tcatggttaa cactcttata ttaatatgtt ttattacgat aaatgaagta
    10141 ctattttagc aagtattttt ttgtaacaca ctatttacag cctgacatat acttatacag
    10201 taactatcgc caataatact tttaatgtac agttatgatg gtgacatgaa tattttttat
    10261 aaatattgat acgtttattt gtatagttat ttttttaaaa aatatcgtta atattatctt
    10321 gacatttaaa aatgcaatct acagtctacc actcaatatt tttttttgcg tattaaatac
    10381 tatatgagtg tataaaggac actgaccatg aataagtata ttgcacaagt agcagttgtt
    10441 acgcttcttg ttttttttac atactcagca tttgctcagg aaaatggctt aacttcgccg
    10501 ccttccagtg atattcctgt tagcgatata ccagataaca atactgctgt taccaataca
    10561 cctgatgcaa ctgttaacaa ttcaaccagt tcgtggaatc cttttaccaa aggcaatggc
    10621 ccaacaacac ctacattagc catgtttatt gcagtcccgg ttattacaac agtatatggc
    10681 atggaaacat gggattgggg cgaacaaaaa aatcccgagc ttgctgatga aggttttttt
    10741 ggtaagaata ccgactctgg tggtgctgac aaagcaggac atgcctatgc acactattta
    10801 tcattcaggg tttttcatgc ctactatgat tggtccgaaa atggaagaaa taccaagtgg
    10861 ttttattcaa ttgcgacagc aaccttttta ggtgttttta tagaagttgg cgatgcattc
    10921 accggcaaat atggattttc gtatgaagat gttgttgccg atgttgccgg gattggtttg
    10981 ggtatactat tagaatattc gccaacactg gactcgctta ttggatttag ctggcagtat
    11041 tggcctactg aaggctacac cgaccgctgg ggagcaaacc ctttgcacat gacaagcgat
    11101 tacagcggtg caaaatatgt tcttaatttt agattagctg gactgcaaaa tctgggttta
    11161 cgattgccca actttttgcg ctatatacag cttgattttg gctatttcac ccgcaactat
    11221 gttcgctggg ataaaaccga cgatgacccc taccgaagcc tgtactttgg catctcatta
    11281 aacttcatgg aagtggtaaa agactttttt gataatcccg aaagcagaac ctgtaaaatt
    11341 ttgcagcaac cgtttaaata ttaccatata ccggcaggat at
//
LOCUS       DAOSOX010000074        11309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5731_length_11309_cov_7.309135, whole genome shotgun sequence.
ACCESSION   DAOSOX010000074 DAOSOX010000000
VERSION     DAOSOX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5731_length_11309_cov_7.309135"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..1521)
                     /locus_tag="PLX22_06765"
     CDS             complement(301..1521)
                     /locus_tag="PLX22_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19638.1"
                     /translation="MLNKRMYSFSLLLLLYLSQGIPFGFQVTALPVLLRQSGISLTII
                     GFSSILALPWMLKIVWAPMVDRFWFKKVGRRKSWLIPLQLLMFMCAFSASYIPITYNP
                     ALFAGIIFVMNLCASMQDIAVDGLAIDVLKEQDLGYGNAIQVVGYKIGMLISGGFLVW
                     LSSYTGWQCTFIIIYSIIAVPLILLIFYKERLLSHTQVKRITFTDVMVHLKNAMAGKG
                     ILFIIFLIATYKSGETLIDGMFKPFLVDCGFTASQIGLWQGVYGMGASISGSIIGGIV
                     SSRFGVIKGLWITLIFRLVPLFGEWYCTTIVPSPSVVILITIAEHLFGGMLTTAMFAF
                     MMYCVDSVIGATHYTLLATIEVIGKSPGMWFSGLLADTIGYSYLFALGIVVSFIPLVL
                     MVPIKKINNTSYVR"
     gene            complement(1540..2034)
                     /locus_tag="PLX22_06770"
     CDS             complement(1540..2034)
                     /locus_tag="PLX22_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19639.1"
                     /translation="MDNDKAAGILICTIIVLFGIHSVTFALNIGYNQLMLGKQYNDVK
                     DILHYEEYEYNEFVIGSIRVIQYHNHEIDGLLIFDLFNRLYTIIVTLPVDEKQYSQLH
                     EHLTKKYGTFTLDNAMYCCTIGIYRIGLYYNKATNMVTIEYNHTLPLFTPAEKQVYNP
                     FSKF"
     gene            complement(2064..3176)
                     /locus_tag="PLX22_06775"
     CDS             complement(2064..3176)
                     /locus_tag="PLX22_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19640.1"
                     /translation="MEYTIVQYNKHHQIIINEYNRKDFIAVCSELLYRPKKLFIGLGD
                     FLEIGDYNQLLDSIFYDEESAIIENFLDAINRKLSEEGQIPFSFLKLAVYYPDYLLKD
                     VPTQCAITVGSKTRLFPGAVHFINFINNYNPMILTAIPYEIANEIVKRVGSDDNHLVS
                     TEYKKIISQKNITLFAGDIEKFISGERKIIEIEKYMALSNTKDEDVLYVGRGESSVIT
                     FSKINSIAFNPSDSIIPHSHITVYGSSLESLLVLFNDSPEINKFLLSEIGEHILPSLI
                     VLSDVKEKPADIIELEKQHRRLQANIIGQRIEHDIESYTTLKRDIELSLSAYPFNFQA
                     VKEMVKERLEKFKINPKEFIDEICSIAQKRYQNWGM"
     gene            complement(3369..4727)
                     /locus_tag="PLX22_06780"
     CDS             complement(3369..4727)
                     /locus_tag="PLX22_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOT19641.1"
                     /translation="MINVDSVVLRRKPSYPLSQWHNDYNNKKVTAEEAAKIIQSGDRL
                     FMGGGTGIPASFATALGKRAGTVNNVTIYQGYAMGLYDYMKPENKESFHIETMFVGPM
                     ERVCMEWKMAHYKPMHLSDIPEAGLDARCNKVAFTATPPDEDGFMNLSCFGAFLPKKE
                     VLCKADTIIAEINTNTPWLNSVDFLVHVSEVDYIIEHDTQLFELPEIPITDVEKKMAE
                     MIADLIPDGATIQLGLGGLANGIGNLLYTKKDLGMHAEVITPSVMRLIEAGVLNGINK
                     TLYPEKVVGAFAVGTKEFYQYLDHNNNFLFCDIGWVNTPDVIALNDNFVSINNALILD
                     LTGQVAAESIGTKQYSGTGGQVDFIRGAKKSKGGKSIIALSSTYTDKQGKIHSNIVPL
                     LPLGTVVTTSRTDVEYVVTEYGVANLRMRSIYDRVHALISIAHPDFRDELKYEAAKIG
                     WI"
     gene            5258..5425
                     /locus_tag="PLX22_06785"
     CDS             5258..5425
                     /locus_tag="PLX22_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19642.1"
                     /translation="MINTITNETNINQTSALQKSKDIGKSIVEESRNRLGEVSKTLEE
                     ENKGKNINTQA"
     gene            complement(5513..9031)
                     /gene="nifJ"
                     /locus_tag="PLX22_06790"
     CDS             complement(5513..9031)
                     /gene="nifJ"
                     /locus_tag="PLX22_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT19643.1"
                     /translation="MAKRIMKSMDGNEAAAYISYAFTEVAAIYPITPSSPMAEFIDEW
                     AAHNKKNIFGQVVRVVEMQSEAGAAAAVHGSLQAGTLTTTYTASQGLLLMIPNMYKIA
                     AELLPAVFHVSARSISAQALSIFGDHQDINAARQTGFAMLASGGVQEVMDLAGVAHLS
                     AIKSRIPFLHFFDGFRTSHEIQKIEILSYEDLARLIDYNKLNEFKNNALNPEHPVTRG
                     TAQNPDIYFQTREVSNIFYQTVPDIVEEYMQEISKLTGREYHPFIYYGAHDAEHIIVA
                     MGSITETIRETIDYLLQQGEKVGLIICHLYRPFSTQYFFKVLPESVKRIAVLDRTKEP
                     GSIGEPLYLDVRAAFYGMDNCPTIVGGRYGLSSKDTTPAHIIAVYNNLKLNDPKNNFT
                     VGIVDDVTFTSLPVPEDTSVVPEGTFEGKFFGLGSDGTVGANKNSIKIIGDNTELYAQ
                     AYFAYDSKKSGGVTVSHLRFGKKPIRSTYLVNNPDFVACHVPAYLEKYDILKGLKKGG
                     AFLLNSLWDEEETKKQLPDHIKRFLAENNIKLYIINATQISENIGLPGRTNTIMQAAF
                     FKVSNVIPFEIAEKEMKKAIEKSYGRKGADVVSKNMAAVDEGAKQVKEVAVPDAWKTI
                     AVPGKPVFEELPPFIQEIVIPMNSLEGDSLPVSAFLPRPDGTFPPGTTQYEKRGIAIN
                     VPEWQPDNCIQCNQCAYVCPHAVIRPFLLNDDEMRKAPEGMLTLDAKGKGLEGLRFRI
                     QISPLDCTGCGNCAEECPAKEKALIMKPLQREIEQQKYWDFIVKNVTYKDNLINKFTV
                     KGSQFCQPLFEFSGACAGCGETPYIKLITQLFGDRMMIANATGCSSIYGGSAPATPYC
                     INAEGRGPAWASSLFEDASEYGYGMYLGVTQQQNTIAQLLEQVAKTTSNTQLKAASLE
                     WLAGRNNAKASREATQKLLPLLKGDSSLEASEVLKLAQYLEKKSIWIFGGDGFAYDIG
                     FGGLDHVIASGEDVNILVLDTEVYSNTGGQSSKATPIAAQAKFATSGKRVRKKDLGMM
                     AITYGYVYVAQVAMGASQMQYMRAITEAENYPGPSVIIAYAPCINHGLREGMGKSQLE
                     EKKAVECGYWSMWRYNPLLEKEGKNPFILDSKEPNWDKFKDFLMGEVRYSALKKEFPE
                     VAEDLFNAALDSAKWRYNNYKRLASN"
     gene            9448..11007
                     /locus_tag="PLX22_06795"
     CDS             9448..11007
                     /locus_tag="PLX22_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HOT19644.1"
                     /translation="MANVFCRDTIGDIVRRSSLRFGEKIAISFRNTNLSFKQLEEECC
                     KFAHLMQQYGIEKGDRVAMISYNSHYYPISFVGLSKIGAIQVPINYMLNADEMAYIIN
                     HSRSRLVIIEDNLYNVIASLVDKCPAVKNWSYIPLEGGDIPEGFDNLITMLNDMSSAD
                     IYTDVYAEDIAQIPYTSGTESKPKGAMLSHRALMSQYFSCIIEGEYKPDDISLHALPL
                     FHCAQLHCFLMPFLYIGATNVILHKADAVGMMKLIEQYHINHMFSPPTVWIGIINHPE
                     FKNYNLNSLKKAAYGASIMPVEVLKRLTEVFSGLRLWNYYGQTEMGPVATILKPDDQL
                     KKPGSAGKPVLNVETALLDDDGHYVSTGEIGEIVHRSGHVMTGYLDDSEKTSEAFAFN
                     WFHSGDLGKFDEDGFLYVVDRKKDMIKTGGENVASREVEEVLYQHPAIAEVAVFGLPD
                     PKWIELVCAAVILKKDMQATNEEIIEFAKQRLAGFKTPKKIFIMDALPKNPSGKILKR
                     ELKHTLSVNVN"
BASE COUNT         3709 a         2140 c         1783 g         3677 t
ORIGIN      
        1 tctcttttta taaggaaaat ggcatacatt gctgccaata ccactgggga ccaccaccag
       61 aagtactcca gcagaacgct tagttgctga ttatactttt atttttgtat attatcataa
      121 actaatcata atagtaaaat catactatca agtaaccttt tttaaaattc ttgcatatgt
      181 ctctatcaac cttttttatt tttttttatt aagacgaata gatatcgttc taaggaagtt
      241 aacgtttgaa actatgaaga atgcttataa tattgctggt tgatgccata ttaccaataa
      301 ctatcgaaca taacttgtat tattaatttt ttttattggt accattaaaa caagtggtat
      361 aaacgaaaca acaattccca aagcaaaaag atatgaatac cctatagtat ctgctaaaag
      421 tcctgaaaac cacataccgg gtgatttccc tataacctct atggttgcaa gaagagtata
      481 atgggtagca cctatcacag agtcaacgca atacatcata aaggcaaaca ttgcagtggt
      541 tagcatgcca ccaaataggt gctcagcaat ggttatcagt ataacaactg atggtgacgg
      601 tactatcgta gtacaatacc attccccaaa caacggcacc aaacggaaga taagcgttat
      661 ccataatcct tttataacac caaagcgaga agatacaatg ccaccgatga ttgatccaga
      721 gattgatgct cccatcccat atacgccctg ccacaatcca atctgagaag cagtaaaacc
      781 acaatccacc agaaaaggtt taaacatccc atcaattaat gtttcgcctg atttataggt
      841 tgctatcaaa aaaataatga aaagtattcc tttacctgcc atggcatttt taagatgcac
      901 catcacatcc gtaaatgtta tacgtttaac ctgagtatgg cttaacaacc tttctttata
      961 aaaaataagt aaaatgagcg gtacagcaat aatactgtat atgataataa atgtacattg
     1021 ccatccagta tatgaactta accatactaa aaagccgcca ctaataagca tgcctatttt
     1081 atatcccact acctgaatag cattgccata tcctaaatcc tgttctttta atacatcaat
     1141 ggctaaacca tcaacagcta tatcctgcat tgaggcgcac aaattcataa caaaaataat
     1201 cccggcaaat aaagctggat tgtatgtaat aggaatatat gatgcactaa acgcacacat
     1261 aaacatgagc aactgcaggg gtataagcca tgattttcgt ctgccaactt ttttaaacca
     1321 gaatctatca accattggtg cccacactat tttgagcatc cacggcaacg ctaatatact
     1381 gctaaatcct atgatagtta atgatatacc tgattggcgc aacaataccg gtaacgcagt
     1441 aacctgaaaa ccaaaaggga taccctgtga aagatagaga aggagaagaa gggaaaaact
     1501 atacattctt ttgttgagca ctactgaata ccgtctttat caaaactttg agaatggatt
     1561 gtatacctgc ttttctgctg gagtaaataa tggcagggta tggttatatt cgatagtaac
     1621 catgttagtt gctttattgt agtataatcc aatcctgtat atacctatag tacagcagta
     1681 cattgcatta tcaagagtaa acgtgccata tttttttgta aggtgttcat gcaactggga
     1741 atattgcttc tcatcaacag gtagtgttac gatgatagta tataacctat taaaaagatc
     1801 aaagattaat aatccatcta tctcatgatt atgatattga ataactctga ttgaacctat
     1861 tacaaactca ttatattcat actcttcgta gtgtaagata tccttaacat cattgtattg
     1921 cttacctaac attagttgat tatagccaat attaagagca aatgttacag aatgaatacc
     1981 aaacaatact ataatggtac atattaaaat tcctgctgct ttatcattat ccataccatt
     2041 aaatccctcc cttaagaaaa atattacatg ccccagtttt gatacctttt ttgtgcaata
     2101 gaacagattt cgtctataaa ttctttggga tttatcttaa acttttccag acgttcctta
     2161 accatctctt tcacagcctg aaagttaaag ggatatgcac tcaatgacaa ttcaatatca
     2221 cgtttcaatg ttgtataact ttctatgtca tgctcaattc tttgaccaat aatatttgcc
     2281 tgcaatcttc tatgttgctt ctctaactcg ataatatcgg caggtttttc tttgacgtct
     2341 gataatacaa taagtgatgg taatatatgt tcaccaattt ccgataacaa aaatttatta
     2401 atttccggtg aatcgttaaa caataccagc agcgattcca gagaagaacc atataccgtt
     2461 atatgtgaat gaggaattat agaatctgaa ggattaaaag caatagaatt aatttttgaa
     2521 aatgtaataa cacttgattc tccccttcca acatacagca catcctcatc ttttgtattg
     2581 gatagtgcca tatatttttc aatttcaatt atcttacgtt caccagaaat aaatttttca
     2641 atatcaccag caaataatgt aatattcttt tggcttataa tttttttata ttcagtagac
     2701 actaaatggt tatcatcaga accaacacgt tttacaattt cattagctat ctcataagga
     2761 attgccgtta gaatcattgg gttataatta ttgataaaat ttataaaatg cactgcacca
     2821 ggaaaaagac gtgttttgga accgacagta atagcacatt gcgtgggaac atcttttaat
     2881 aaataatcag gataataaac tgctaatttc aaaaaagaaa atggaatttg cccttcttct
     2941 gagagcttcc tgtttattgc atctaaaaaa ttttcaatga ttgcactttc ctcatcataa
     3001 aaaattgaat ccagcaactg gttataatca ccaatttcaa gaaaatcacc aagtcctata
     3061 aacagctttt taggtcggta tagcaactca gagcaaacag caataaaatc tttccgatta
     3121 tactcgttga taattatttg atggtgtttg ttatattgaa ctattgtata ttccatatat
     3181 aaactcttaa tgatcttccc taactaacta actaactaac taaataaata aatagtaata
     3241 ctttaaacat caataaacat tacaatatca ttataataga ataaatagga tattgtaata
     3301 ttctataaaa tagaattaca ggtcattaag aatttttcaa gaaattaatt caattacttt
     3361 atgatatatt atatccaacc tattttggca gcttcatatt tcagctcatc tctaaaatca
     3421 ggatgcgcta ttgatatcaa tgcatgtact ctgtcataga tacttcgcat acgaagattg
     3481 gcaacaccat attctgtgac tacatattct acatcagttc gtgatgttgt taccacagtg
     3541 cccaatggca aaaggggaac aatattgcta tgtatttttc cttgtttatc ggtatatgtt
     3601 gaactaagcg ctataatact tttaccaccc tttgacttct tagcaccacg tataaaatct
     3661 acctgcccac cagttccgct atactgtttt gttccaatgg attcagcagc aacctgcccg
     3721 gttaaatcaa gtatgagcgc attattgatc gaaacaaaat tatcatttaa agctataaca
     3781 tccggtgtat taacccatcc aatatcacaa aacaaaaaat tgttgttatg atcaagatat
     3841 tgataaaatt cttttgtgcc cactgcaaat gcgccaacaa ccttttcggg ataaagtgtt
     3901 ttgttaatac cattaagaac acctgcctca ataagtcgca taactgatgg ggtaataacc
     3961 tcagcatgca tccctaaatc tttttttgta tacagtaaat tgccaattcc atttgcaagc
     4021 ccacccaaac caagctgtat ggttgctcca tcagggatca aatccgcaat catttcagcc
     4081 atcttctttt caacatcagt tatgggaatt tcgggcagtt caaataattg agtatcatgt
     4141 tcaatgatat agtccacctc agaaacgtgt acaagaaaat caacgctatt gagccatgga
     4201 gtatttgtat ttatttcagc tataatagta tcagctttgc atagcacctc tttttttggt
     4261 aaaaaagctc caaagcatga taaattcata aatccatctt catcaggtgg tgttgccgta
     4321 aacgctactt tgttacacct tgcatccaat cctgcctcgg gtatatcgct taaatgcatg
     4381 ggtttgtaat gtgccatctt ccattccatg caaacacgtt ccataggacc aacaaacatt
     4441 gtttcaatat gaaaactttc tttattttcg ggtttcatgt aatcatacaa acccattgca
     4501 tatccctgat agatggttac attgttaacc gtccctgccc ttttaccaag agcggtagca
     4561 aacgaagcag ggataccagt accaccaccc atgaacagcc tgtcaccaga ttgaataatc
     4621 tttgcagctt cttctgctgt aacctttttg ttattataat cattatgcca ttgtgacagt
     4681 ggatacgatg gcttgcgacg caaaacaact gaatcaacgt ttatcatact ataccgcaat
     4741 aatttaaaaa ttttaactac tatccattgt agttactgta ctgctcattt atcaacttaa
     4801 caatacatat agatattatt atatttatca atagaaaatt taatattatc aatattaatt
     4861 tatatgcata tacattatat attatatata tttgtatatt ttttccattt atttttatgg
     4921 cgatagttac tggtaataaa attttatttg gtatgaaatt tcttccattt tctatttatt
     4981 tacatttaat gtaattcact tttgccatat actcaataat tgacatccat aatcattgat
     5041 gattttgatt ggttgcgttg caaatagtca tataatggtt tttaaataat aaaagcaatc
     5101 tattacattt atacataatt tatttgactt tattacctat cttatatata ttaaagtaaa
     5161 ataataagtg gcgatagttt tttaaagtaa tatattaata aatgttagat ttcaattatg
     5221 ccatttcaac aaatcagcaa aaaacaaggg agggtttatg attaatacaa tcactaatga
     5281 aacaaacatc aatcaaacaa gtgcgttaca aaaatcaaag gatataggga aaagcatagt
     5341 tgaagagtcc cgaaacagat tgggcgaggt ttctaaaact ctggaagaag aaaataaagg
     5401 aaagaatata aatactcagg catagaaata ttttacgagg tgatatccaa aggataaagt
     5461 attaaaaagg ctccctatta ttataggtta ttacagggag cctttattat tcttaatttg
     5521 acgctaatcg tttataatta ttgtaacgcc atttggcgct atccagtgct gcattaaaaa
     5581 ggtcttcggc aacttcaggg aattccttct tcaaagcaga atagcgcact tcacccatca
     5641 aaaaatcttt aaatttatcc caattaggtt ctttggagtc cagtataaat ggatttttcc
     5701 cttctttttc cagcaatgga ttgtaacgcc acattgacca gtagccacat tcaacagctt
     5761 ttttctcttc aagctgtgat ttacccattc cttcgcgtaa tccatgattg atacatggtg
     5821 cgtatgcaat gattacggaa ggtcctggat aattttccgc ttcggttata gctcgcatat
     5881 actgcatctg gctggcaccc attgctacct gagccacata cacgtaacca taggtaatag
     5941 ccatcatacc aagatctttc tttcgtacac gcttaccgct tgttgcaaac tttgcctgcg
     6001 ctgcaatggg tgtagctttg gatgactgtc ctcccgtgtt ggaatatacc tcagtatcca
     6061 ataccagaat attaacatcc tcgccagatg caatgacatg atctaatcca ccaaacccaa
     6121 tgtcatatgc aaatccatcg ccaccaaata tccagattga ttttttctca aggtattgag
     6181 ctaattttaa cacctcgctt gcttccaggg aactatcgcc ctttaataag ggtaaaagct
     6241 tttgcgttgc ttcacgtgaa gcctttgcat tattcctgcc tgcaagccat tctaacgatg
     6301 ctgctttaag ctgtgtatta cttgtagttt ttgctacctg ttccagcaac tgagctatgg
     6361 tattttgctg ctgcgttaca ccaaggtaca tgccataccc atattcactt gcatcttcaa
     6421 ataatgaact tgcccatgca ggaccacgtc cttctgcatt gatgcaatag ggagttgcag
     6481 gagcagagcc tccatagatt gatgaacaac cggtagcatt agctatcatc atccggtcac
     6541 caaataattg tgttatgagt ttaatataag gggtttcgcc gcaaccggca catgcaccag
     6601 aaaactcaaa taaaggctga cagaactgac tacctttgac agtaaattta ttgataagat
     6661 tatctttata ggttacattt ttgacaataa aatcccagta tttctgctgc tcaatttccc
     6721 tttgaagagg cttcatgata agagcttttt cctttgcagg gcattcttca gcacaattgc
     6781 cacatccggt acaatccagt ggactgattt gaattctaaa tcgtagtcct tcaagtcctt
     6841 tacctttagc atccagtgtt agcatccctt caggagcttt acgcatttca tcatcattga
     6901 gaagaaatgg acgtataaca gcatgagggc aaacatacgc acactgatta cattgtatac
     6961 aattatcagg ctgccattct ggaacattga ttgctattcc gcgtttttca tactgcgttg
     7021 tccccggagg aaatgtgcca tcaggccttg gcaaaaatgc acttacaggg aggctgtcac
     7081 cttcaagact attcatgggt ataactattt cttgtataaa tggtggcaac tcctcaaata
     7141 ccggttttcc tggcactgca atagttttcc atgcatcagg caccgctact tccttaacct
     7201 gttttgcacc ttcatcaacg gcagccatgt ttttgctaac aacatctgca cctttcctgc
     7261 cataactttt ttcaatagct tttttcatct ctttttcagc aatctcaaac ggtataacat
     7321 tgctaacctt aaaaaatgct gcttgcataa tagtattggt tctgccgggg agcccaatat
     7381 tttctgaaat ctgtgttgca ttgatgatgt acaatttaat attattctca gccagaaatc
     7441 gttttatatg atcaggtaat tgctttttag tttcttcttc atcccataat gaattaagca
     7501 ggaatgcccc gccttttttc aaccctttga ggatatcata tttttcaaga tatgctggta
     7561 catgacaggc tacaaaatca ggattattta ccagataggt agaacggata ggctttttac
     7621 caaatcgaag atgagatact gtgacaccac ccgatttttt tgaatcatac gcaaaataag
     7681 cctgagcata cagttctgta ttgtcaccta ttatctttat agaatttttg ttggcaccaa
     7741 ccgttccatc agaacccaag ccaaaaaact taccctcaaa cgtaccttcg ggaacaacac
     7801 tggtatcctc tggaactggt aatgaagtaa atgttacatc atctacaata cctactgtaa
     7861 agttattttt gggatcattg agctttaaat tattgtatac tgctataata tgagctggcg
     7921 ttgtatcctt tgagcttaat ccatatcgtc cgccaacaat agttgggcaa ttatccatac
     7981 cataaaaagc agctctgaca tctaaatata acggctcacc aattgaacct ggctccttgg
     8041 ttctatcaag gacagctatt cgttttacgc tttcaggtaa aactttaaag aaatattgtg
     8101 ttgaaaatgg gcggtacagg tgacatatta taagcccaac cttttctcct tgctgtaaca
     8161 aatagtcaat tgtttctctg atggtttcag taattgatcc catagccact ataatatgtt
     8221 cagcatcatg agcaccataa taaataaatg gatgatactc tctaccagtt aatttgctta
     8281 tctcctgcat atattcttct acaatatcag gtacagtctg atagaatata ttgcttacct
     8341 cacgagtttg aaaatagatg tcagggtttt gagctgtgcc acgtgttacc ggatgctctg
     8401 gattcaatgc attattctta aattcattta atttattata atctatcagc ctggctaaat
     8461 cttcataaga aagtatttca attttttgta tttcatgaga tgttctaaat ccatcaaaaa
     8521 aatgtaaaaa cggtatgcgt gatttgatag cacttagatg agcaacaccg gctaaatcca
     8581 taacctcctg cactccgcca cttgcaagca tagcaaaacc agtttgacgt gcagcattaa
     8641 tatcctgatg atcaccaaat attgatagtg cctgagcaga aattgaacgc gcacttacat
     8701 gaaaaactgc tggtaataac tcagcggcaa ttttatacat attaggtatc attaaaagta
     8761 aaccctgtga agcagtatag gttgtagtta atgtacctgc ctgcaatgaa ccatgaactg
     8821 cggccgcagc accagcttct gattgcatct caacaacacg aactacctgt ccaaatatgt
     8881 tctttttgtt atgagcagcc cattcgtcta taaattcagc cataggtgaa gatggtgtaa
     8941 ttggatagat ggcagccacc tcggtgaaag catatgaaat atacgcggca gcctcattac
     9001 catccattga tttcataatt cgttttgcca tgaaagcttt ctcctttctg ttttattata
     9061 taataattat aaacataaaa aatcaattca gcctgcatta acacctatta aatataaaca
     9121 attgctacta tgtatgatta gaaaaagatc agggcgtgat tattaccttc ataaatcatt
     9181 atgaaacaag tcaataaaaa aaacaattaa taaagatcaa aaatatagta aaaatctaat
     9241 tataattcta ccaatgttta tattatatcc tattattaat aaaaaatagc aataatatat
     9301 aacaattcca tttaattctc atatgcaata gtccattcta tccaatcttt gactatttta
     9361 tattttattt acaaatattg cttgaatgca aaaggtatta tttcatattt gttatatatc
     9421 atatcataat ataaacggag gatctttatg gctaatgttt tttgcagaga tacaattggt
     9481 gatattgtac gacgctcttc tttacgtttt ggggaaaaaa tagcaatttc atttcggaat
     9541 accaatcttt catttaagca gctcgaagag gaatgttgta aatttgctca tcttatgcaa
     9601 cagtatggta ttgaaaaagg tgacagggta gctatgattt cttacaattc acattattat
     9661 ccaataagtt ttgtaggact ttcaaaaatt ggagctattc aggtaccaat taattacatg
     9721 ctcaatgcag atgaaatggc atatattatt aatcattcca gatccaggct tgttatcata
     9781 gaagataatt tatacaatgt tattgcttca ttagtcgata aatgtccagc tgtgaaaaat
     9841 tggagttata ttccattgga aggtggtgat attcccgaag gatttgacaa tcttattaca
     9901 atgctgaatg atatgtcatc agcagatatt tatactgacg tttatgcaga agacatcgct
     9961 cagattcctt ataccagcgg tacagaatca aaaccaaaag gagcgatgct atcacaccga
    10021 gcattaatgt cacaatactt tagctgtatt atagaaggag aatacaaacc cgatgatata
    10081 agtctccatg cattaccact tttccattgc gctcaacttc attgtttcct tatgccgttt
    10141 ttatatattg gcgctactaa cgtgatattg cacaaagctg atgcagttgg tatgatgaaa
    10201 ttaattgaac agtaccatat taatcatatg ttttctccac caacggtatg gattggtatt
    10261 attaaccatc ctgaatttaa aaattataac ttaaacagtc ttaaaaaagc tgcatatggt
    10321 gccagtatta tgcctgttga agtattaaaa cgtttaacag aagttttttc tggtcttagg
    10381 ttgtggaact attatggaca aaccgagatg ggaccggtag caacaatctt aaaaccggat
    10441 gaccaattaa aaaaacctgg ttcagcaggt aaacctgtgt tgaacgttga aactgcctta
    10501 ctggatgatg atggtcacta tgtttctact ggtgaaattg gagagatagt tcaccgttca
    10561 ggacatgtta tgactggtta tttagatgac tctgaaaaaa cttcagaggc atttgcattt
    10621 aattggtttc atagtggaga tttaggtaaa tttgatgaag atggattcct ttatgttgta
    10681 gatagaaaga aagatatgat aaaaacaggt ggcgaaaatg ttgcatctcg tgaagttgaa
    10741 gaagttttat atcagcatcc tgcaattgct gaggtagctg tatttggact tcctgatcct
    10801 aaatggattg aattagtctg tgccgccgtg atattgaaaa aagatatgca ggcaactaat
    10861 gaagaaataa ttgaatttgc aaaacaacgt ttagctggat tcaaaacccc aaagaaaatc
    10921 tttattatgg atgcattacc caaaaatcct tcaggaaaaa tattaaaacg tgaattgaaa
    10981 catacattat ctgttaatgt aaattaatat tacacttcat attcttaaaa attgcagcag
    11041 gtacaataat tctgttgcaa ttttttttta ttatctattt agtgtcacct ataaaaactt
    11101 ctcattatca ttaaactgaa ttactcatat attttcaaag aactatatag actttagaga
    11161 tagcgatcag atattattac aaaactgaaa aataaggggt aatacgatgt atccaatact
    11221 attccaatac aaattcataa caattggtgg atatggtgtt atgcttgcaa tcggtattta
    11281 tttagcctat cttttattag aacgagagc
//
LOCUS       DAOSOX010000075        11235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5766_length_11235_cov_7.199463, whole genome shotgun sequence.
ACCESSION   DAOSOX010000075 DAOSOX010000000
VERSION     DAOSOX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5766_length_11235_cov_7.199463"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLX22_06800"
     CDS             complement(<1..191)
                     /locus_tag="PLX22_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOT19645.1"
                     /translation="MTKVSNETTEKNNNNNRKNGNGDYSRKNNNGTIQEGRLDLAELK
                     SKTISALMEIARSMGIEGIS"
     gene            complement(866..2023)
                     /locus_tag="PLX22_06805"
     CDS             complement(866..2023)
                     /locus_tag="PLX22_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT19646.1"
                     /translation="MSSQITALPIIETNPEGAYFYDSKGKRYIDCYSGAGMFNLGRHV
                     KELSNTLCEQADKSDQGNFVLISQEKALLASKLVSFVPAPMMGVFFSVGRGEAMDFAC
                     KLARGFTGRAQLITIDGAYYGETGFSISLSDGDKKKIFGQIPQITTIPFGNIQALEEK
                     VTRNTAAVITEIIQVENACRKASEGFFKIMRQVCNATGALMVIDETQTGMGRTGKKFA
                     FEHYGIQPDILVIGEALGAGIFPIAATVFTKKVQKFMHKNPLIHLSTFGGHDLGCRVV
                     YEALELYEKIKPWENAYAVGQYLMDKLLTLHRIYPDAIISVNGQGLVAALTLKNDDMA
                     NRASVKLAREGIFAVHAKVATDTILLRPALTISKHDVDDIVRAMSKIINSL"
     gene            complement(2027..3439)
                     /locus_tag="PLX22_06810"
     CDS             complement(2027..3439)
                     /locus_tag="PLX22_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT19647.1"
                     /translation="MALWNDTLSSKNKKYIMSLFSKHISKGQTKTMRAAHLDFIETRR
                     KGIGFIDGLFRRHIIDCFTSAGCFNVGRGNPEIIKALCTALQKYDMGTNLLPSNNKKQ
                     FIKKLQAIAPGDCKGIIFASGGGDAIDCAIKLARGATGRKKIIAMKKAYHGHTGYSLS
                     ANGKDYYRHLFEPLEPNFAFVPFGDIEAVKQIAGMDTAAIILEPVQGEGGIFVGDDYY
                     LKRLRTVCNQFGIMLIFDEIQTGFGRTGKLFASQHSGVVPDIMVLAKSISGGIFPNAV
                     VMYRTTPLLNNYVKSNHDFHQSITGGSDHGCAVGSAVIDYVVKNRLWENAAKMGAILM
                     DGLLTIQHENPTIIKEIRGKGLMIGVEYTHEYMGALMADSLARHGIFAIYSGNQPQVM
                     RFMVPITISEQEVQKMLTIIQKAIGTMKLYLPLTKPLSKIPILSRLLDDIHIQIAVFD
                     IVRYCEDILGITKRNYSKEA"
     gene            3578..4189
                     /locus_tag="PLX22_06815"
     CDS             3578..4189
                     /locus_tag="PLX22_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19648.1"
                     /translation="MHTYMGRRSLKNIRQEEIARALYRCLLKKPFSKTTIKDIAKEAG
                     VNHGMLHYYFKSKEEILLYFLDWVVAIHLDGFKQWYKSRHLDNLPFDRALTLVLDYTI
                     KKITLNEDLAKLFIEIWEIALYNKTVKKKLQYVYHEWIDQLAIIIGKETNEKIATVLS
                     AATIAFLEGMSLFSIMLRGKYPINTIVDYFEKRIETIIKMNIM"
     gene            complement(4288..5298)
                     /locus_tag="PLX22_06820"
     CDS             complement(4288..5298)
                     /locus_tag="PLX22_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HOT19649.1"
                     /translation="MFLKKLLYPALLFFFITNTIIICLPDGAHATISAYMPYYAVYEE
                     VNGTNTYSYIIIRRFKLNNTGTYLCINPYTLTTGLLKPAHSAIKKQSLQSILAMFPNS
                     PYATLYSLAQKNSKRMFNAGITHFNQLDTIVVSFDLCPSKKQLDTRFIEQFIDYNKNQ
                     LPAPVTICISGNWLLKHEDDLQWLKQLAFNKQLDIVWVNHSYSHFYSRSLPIKKNFLL
                     KEGTNIACEVLQNEKIMLEHNIIPSIFFRFPGLVSNQKIYSKVMDYGLIPLGSDAWLA
                     KGKMPRGGSILLVHLNGNDKKGTRILLQYMQSVHQPGIIASITHCNRNGNTCTIKKQT
                     KL"
     gene            5527..>5885
                     /locus_tag="PLX22_06825"
     CDS             5527..>5885
                     /locus_tag="PLX22_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT19650.1"
                     /translation="MMKLIVQKYGGTSVGTVERIKAVASRIKRYVENGYSVVVVVSAM
                     GKTTDQLIDLAKQITQNPDKREMDMLLSTGEQVSIALLAMALHDSGINAISYTGSQVK
                     LITDGNFSNARIQSIST"
     assembly_gap    5886..5985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5986..6486
                     /locus_tag="PLX22_06830"
     CDS             <5986..6486
                     /locus_tag="PLX22_06830"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT19651.1"
                     /translation="VMPREEMMEKFVISGVTSKDDEAKITIRDIPDRPGVASMLFGEL
                     GKQKVYVNMIVQSTGKDGKASISFTVLKNDLKKALTICEGLKATMGATSIDYKENIAI
                     VSAVGVGMLSSWGVAGRMFDVLAKHNINIEMISTSEIGISCVIDSMYTELAVKAIHKE
                     FIEGNE"
     gene            6479..7645
                     /locus_tag="PLX22_06835"
     CDS             6479..7645
                     /locus_tag="PLX22_06835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOT19652.1"
                     /translation="MNNKEGIAQINKKKAVTKKLRLGIIYGGKSGEHDVSRCSAASIA
                     EVASKKYEVVCIGITRDGRWYVQNAIHIENHPSFGNVLAIHEHGSWAIDTSSSDGSLH
                     LLERVTGEEVIVDVVFPIVHGTYGEDGTLQGLLEMVDVPYVGPGVLGSAVGMDKDIAK
                     RVLMNAKGFAIVPFKTIRINEMNPHNKQTILRMVERMLPVFIKPANAGSSVGISKVTS
                     KGMIEESLSLAFQYDTKVIVEKAIDCREFEVSVMGNEEPVASVVGEIIPQHEYYSYKA
                     KYLEKDGATLAIPATISKSLEHNLQKAACIIFSLLECEGLARVDFFVEKQTNILYFNE
                     INTLPGFTSISMYPKLWDCSGIPYTKLIDTLIHYALKRHRRRKKMVGAFLRLKK"
     gene            7667..>7863
                     /locus_tag="PLX22_06840"
     CDS             7667..>7863
                     /locus_tag="PLX22_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOT19653.1"
                     /translation="MNYRTKKIKGLYVDTVLFDMDGVIVDSMYAHARCWIQVFEEHGV
                     HLDAIDILKREGMSGLESIAD"
     assembly_gap    7864..7963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8112..8825
                     /gene="pssA"
                     /locus_tag="PLX22_06845"
     CDS             8112..8825
                     /gene="pssA"
                     /locus_tag="PLX22_06845"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOT19654.1"
                     /translation="MSVAWIPNTLTLGNLVLGFIAIIFASMNIPHHLSIAGVLILGAA
                     LLDGLDGQVARMLGVASKLGQELDSLADCVTFGIAPGYLAFKGYLQGTWIYILDKPID
                     IGIFIAAIFPVCAAYRLARFNVQHVPNAFDGLPSPIAGIIVALVPLCFINIIIPKLLF
                     IIGFVLVALLMVSTVRYAKPQSFILQKITGIKLLLFILILIALIFMFKQWVIMVIIAL
                     YILSGLLSFIIQLIQDYRY"
     gene            9377..9661
                     /gene="groES"
                     /locus_tag="PLX22_06850"
     CDS             9377..9661
                     /gene="groES"
                     /locus_tag="PLX22_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT19655.1"
                     /translation="MGIKPLGDRVLIEVTEEELPKQGSLYIPDTAKEKPQQGKVLAVG
                     KGRYEDGKWVPLDVKTGDTVLFGKYAGTEVKYGGKEYLIVRENDILAILD"
     gene            9695..>11235
                     /gene="groL"
                     /locus_tag="PLX22_06855"
     CDS             9695..>11235
                     /gene="groL"
                     /locus_tag="PLX22_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888904.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT19656.1"
                     /translation="MAKILQYNDEARRSIYQGVIKLSDAVKVTLGPRGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATILAAAIYHES
                     LKNVTAGANPMSLKRGIDKAVEVAVKFIGDTAREIKDKKEIAQVAAISANNDMEIGEL
                     IAEAMDKVGKDGVITVEEAKSIETHLEVVEGMQFDRGYISPYMVTDPENMEAVLEDAF
                     ILINEKKISTMKDLLPILEKVAQAGRPLLIIAEDVEGEALATIVVNTLRKVIQCVAVK
                     APGFGDRRKAMLEDIAILTKGQVISEDLGLKLENTTMDMLGRAKKVIIDKENTTIIEG
                     AGSQADVQARIKVLKKQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEVELKEKK
                     ARVEDALSATRAAVEEGIVPGGGLTLLYAQKAVAELEKKLDGDEKIGVRIVAKAMEEP
                     MKMIAFNAGVEGSVVVEKAKQEKQGIGFNAATLEWEDLTKAGIIDPAKVVRSALQNAA
                     SVAGML"
BASE COUNT         3449 a         1853 c         2255 g         3478 t
ORIGIN      
        1 gaaattcctt caatgcccat tgatcgggca atttccatga gagcacttat tgtttttgat
       61 ttaagttcag ctaagtctaa acgaccttct tgaatagttc cattattatt ttttcttgaa
      121 taatctccgt ttccgttttt tcggttatta ttattattct tttcagtagt ttcattggat
      181 acttttgtca taaatcacct tttgatacat tatactttat aatagtcaac tgtcctttat
      241 gttattgaga aacaagggca tatttgctta cttggtgtgt aattgttaca ctaatagcgt
      301 tataaaaaaa actcatggca gtgtgtgaaa tcgtaattag ttaataaaat actttgaaca
      361 cttctaaaaa ctgtttttcg tttgatgaca aacaattaaa acaattggga aaagcagttt
      421 tacgaatatg ttattaccac acgggtatta aagaattaaa gatttagtta tccccttata
      481 cctgtaagaa agcatttcat attgtgttgt ttttactatg ttattaaata ttaacgtcat
      541 gataaaatat atatttagct ttttaattta ataattcact gtatccgggc aggataataa
      601 tgtaactgta tgatctattc tcccttaaat atactataaa aaaaataaat ataccacaaa
      661 aaaataaaat atttttttat tttttaccac catataccat aatacaattt cgtcaagaaa
      721 aaatgtaaaa tttaaaaata ttcattcagc agttacattc attctatcac ttaaaccgtt
      781 gcatattttg acattttgaa actatcgctg atagtttgtc aaataaataa tcccacccct
      841 ataggagcag gactattgtt tgcaactata aactattaat gattttgctc atagccctga
      901 ctatatcatc tacatcgtgt tttgagatag tcaatgctgg acgtaagagt attgtatcgg
      961 tagctacttt tgcatgtact gcaaatatgc cttctctggc aagttttaca cttgctctgt
     1021 ttgccatgtc atcattcttt aaagtcagtg cagcaacaag tccctgccca ttgacagaaa
     1081 ttattgcatc gggatatatt cggtgtaatg tcaaaagctt gtccatgagg tattgtccaa
     1141 ctgcatatgc attttcccat ggtttaattt tttcgtacag ttctagtgct tcgtatacca
     1201 ctctgcaacc taaatcatgg ccgccaaatg tagaaagatg aattaacgga ttcttatgca
     1261 taaatttctg cacttttttt gtaaatacgg ttgctgcaat aggaaagatg cctgcgccta
     1321 acgcttcgcc aatgacaaga atgtccggtt gtatcccata atgctcaaat gcaaactttt
     1381 tgcctgtcct gcccataccg gtctgtgttt catcaataac cattaaagca ccggttgcat
     1441 tgcatacctg ccgcatgatt ttaaagaaac cttcacttgc tttgcgacag gcattttcta
     1501 cctgtattat ttcagtaatt accgcagcag tatttctggt tactttttct tcaagtgcct
     1561 gtatattgcc aaatgggatt gttgtaatct gaggtatctg accaaatatt ttctttttgt
     1621 ctccatcgga taatgaaatg gaaaatcctg tctcaccgta atacgcaccg tcaatggtga
     1681 taagctgtgc tctgccggtg aatccccgtg ctaatttaca ggcaaagtcc attgcttcac
     1741 ccctgccaac cgaaaaaaat acacccatca tcggagcagg aacaaacgat acgagctttg
     1801 atgccagcag tgccttttcc tgtgatatga gcacaaaatt tccctgatcg cttttatctg
     1861 cttgctcaca tagtgtgttc gatagttcct ttacatggcg acctaaattg aacatgccgg
     1921 ctccactgta gcaatcaata taccgtttac ccttactgtc ataaaaatat gccccctctg
     1981 ggttcgtttc aattattggt aatgctgtga tttgcgaact cataccttat gcctccttac
     2041 tataatttct ttttgttatc ccaaggatat cctcacaata gcggacgata tcaaatacag
     2101 caatctgtat gtggatgtcg tctaaaagcc gtgatagtat tggtattttt gaaagaggtt
     2161 ttgtcagggg cagatagagc ttcatggtgc caattgcttt ttgtataatc gttaacatct
     2221 tttgtacttc ctgttcacta atggttatcg gtaccataaa acgcattacc tgaggctgat
     2281 tacccgaata tatagcgaat ataccatgtc gggctaaaga atctgccata agtgctccca
     2341 tatattcgtg tgtgtattca acaccaatca taagcccttt gcctctgatc tcttttataa
     2401 tagttggatt ttcatgctga atggttaaaa gcccatccat gaggatggcg cccatttttg
     2461 cggcgttttc ccatagacgg ttttttacca cataatcaat aactgcactt ccaactgcac
     2521 acccatggtc gctgccaccg gtgattgact ggtgaaaatc atggttggat tttacatagt
     2581 tattcagtaa tggtgtagta cgatacatga ccacagcatt agggaaaatt ccaccgctta
     2641 tggattttgc cagtaccata atatcgggaa ccacgccgga gtgctgtgat gcaaaaagtt
     2701 taccagtacg cccaaaacct gtctgtatct catcaaaaat gagcataata ccaaactggt
     2761 tgcatactgt gcgcaatctt ttcagataat agtcatcgcc tacaaagatg cctccttcac
     2821 cctgtactgg ttctaatata atagcagctg tgtccatgcc tgcaatctgc ttaacggctt
     2881 ctatatcacc aaacggtaca aaagcaaaat ttggttccag aggttcaaaa aggtggcgat
     2941 agtaatcctt accattggct gaaagtgaat atccggtatg accgtgataa gctttcttca
     3001 tggctataat ctttttcctg ccggtggcac ctcgtgccaa cttgatagca caatcaatgg
     3061 catcgccacc ccctgaggca aatataatgc ccttacaatc tccaggagct atcgcctgca
     3121 atttttttat aaattgcttt ttattatttg atggtaacag attggtgccc atatcgtact
     3181 tttgcaatgc tgtacaaagt gctttgatta tttcagggtt gcccctgcct acattaaagc
     3241 aacctgccga agtaaaacaa tctataatgt gccgtctaaa caacccatca ataaaaccaa
     3301 tacctttgcg ccgtgtttca ataaaatcta aatgggcagc gcgcattgtt ttggtttgtc
     3361 ctttagagat gtgctttgaa aaaagcgaca taatgtattt tttattttta ctgcttaacg
     3421 tatcattcca tagtgccatg caaacctcct tatatataat atattagttg ggtgactaac
     3481 ataaaaattt tgttctgtca ataaatatat attgacatgt tttaaaaaat attcataaag
     3541 agagttttaa tgcttcttaa tcaaaacggg atacgatgtg catacatata tgggaaggcg
     3601 ttcattaaaa aacatacggc aggaagaaat agcacgggca ttgtatcgct gtcttttaaa
     3661 aaaaccgttt agcaaaacaa caataaaaga tattgcaaaa gaagcgggtg tgaatcacgg
     3721 aatgctgcac tactatttta aaagcaagga agagatactg ttgtatttcc ttgattgggt
     3781 tgttgctatc catcttgatg gatttaaaca gtggtacaaa tccaggcatc ttgataatct
     3841 gccatttgac agagcgctaa cattggttct tgattatact ataaaaaaga taacactcaa
     3901 cgaggatctg gcaaagttgt ttattgaaat ctgggagatt gctctttata ataaaacggt
     3961 taaaaaaaaa ttgcagtatg tttatcatga atggatagat cagcttgcca taattattgg
     4021 gaaagaaacc aatgaaaaaa tagctaccgt gctttcagca gctactattg cctttctgga
     4081 gggcatgtcg ctattttcta ttatgctccg cggaaaatac cccataaaca ctatcgtcga
     4141 ttattttgaa aagcggattg aaacgataat caaaatgaac attatgtaga tagatggcac
     4201 attgcttgtg gtttatagtt tgcggtttat ggtgatggtt atacgatgtt tgattttata
     4261 tagagcggtc cggactatta aaaatattta aagcttcgtc tgctttttaa tggtgcaggt
     4321 attaccattc ctattacaat gtgtaataga tgcaattatg cctggttgat ggacagattg
     4381 catatactga agcaaaatcc ttgttccttt tttatcattg ccgttaagat gtaccagtag
     4441 tatactccct ccccttggca tttttccttt tgcaagccat gcatcagaac ccaatggtat
     4501 aagcccatag tccataactt tagaataaat tttttgattg gaaacaagcc ctggaaacct
     4561 gaaaaaaata gatggtatga tgttatgctc aagcattatt ttttcatttt gtagtacctc
     4621 acaagcaatg ttcgtacctt ctttcagcaa aaaatttttt ttaataggca gtgatcgcga
     4681 gtaaaaatgg ctgtagctat gatttaccca tacaatatcc agttgtttat tgaatgctaa
     4741 ttgtttaagc cattgcaggt catcttcatg tttcagtaac caattgccac ttatacagat
     4801 agtaaccggc gcaggcagtt gattcttatt ataatcaatg aactgttcta taaatcgagt
     4861 atccagttgt ttttttgatg ggcatagatc aaaactaacc actatagtgt ccaattggtt
     4921 aaaatgagtt ataccggcat tgaacatccg ttttgaattt ttttgtgcaa gagagtagag
     4981 cgttgcataa ggcgagttgg gaaacatggc aagtatggat tgcaatgatt gttttttgat
     5041 tgcagaatgt gcaggtttta ataaaccggt tgtaagggta taaggattga tgcaaagata
     5101 ggtgccggtg ttgttaagct taaatctgcg tataatgata taggaataag tattggttcc
     5161 attcacttct tcatacacag catagtatgg catataggca ctgattgttg catgtgcacc
     5221 atcaggtaaa cagatgataa tggtgttagt aataaaaaaa aatagtagtg caggatataa
     5281 taattttttc aaaaacatgg atacaccatt aaatgtttct ttggtaaaag tgtttgttag
     5341 attaacaaat gaagtgaaat agtataaatt gtcaatccaa tattatataa ccagaatata
     5401 ctattatggt gtatataaat gttaatgtaa atattttaca aatcatacat aatttttgtt
     5461 gcaaatgtat ggctactctg tatatagcgc actgtgttga ttaatttttg atagaaaggt
     5521 acaataatta tgaagctcat tgtccagaaa tatggtggca catcagttgg gacagttgaa
     5581 cgaattaaag ctgtagcatc aagaataaag cgatatgtag aaaatggtta ttcggtggtt
     5641 gtagttgttt cagcaatggg caaaacaact gaccagctga ttgaccttgc aaaacagata
     5701 acacaaaatc cggataagcg tgagatggat atgctacttt ccaccgggga acaggtatca
     5761 attgcgttac ttgcaatggc tttacatgat agtggcatta atgcaatatc atataccggc
     5821 tcacaggtta aattgattac cgatggaaac ttttcaaacg cacgaatcca gagcatttct
     5881 actgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtaat gccacgggag
     6001 gagatgatgg aaaaatttgt cataagcggt gtaacgtcaa aagatgatga agcaaagatt
     6061 accatacggg atatccccga caggccaggc gttgcttcca tgctgtttgg tgaattgggt
     6121 aaacaaaagg tatatgtcaa catgatagtg cagtcaacag ggaaagatgg taaggcatct
     6181 atctcgttta cggtattaaa aaatgatctt aaaaaggcat taacaatatg tgaaggatta
     6241 aaagcaacaa tgggtgcaac cagcattgat tataaggaaa atattgcaat agtgagcgca
     6301 gttggtgttg ggatgctgtc gtcatggggt gtggcagggc gcatgtttga cgttctcgca
     6361 aaacataata ttaacattga gatgatatca acttcagaga ttggcatatc atgtgttata
     6421 gattcaatgt ataccgaatt agcggtgaaa gctattcata aagaatttat tgaaggcaat
     6481 gaataataaa gaaggcatag ctcaaatcaa caaaaagaaa gcagtaacaa agaagttacg
     6541 gcttggcatc atctatggtg gcaaaagtgg cgaacatgat gtgtcacggt gttctgcagc
     6601 atccattgct gaggttgcca gtaaaaaata tgaggtggta tgtataggca ttacccgtga
     6661 tggacgctgg tatgtacaaa atgccataca cattgaaaat catccctcat ttggcaacgt
     6721 gcttgcaatc catgaacacg gtagctgggc aattgataca agctcaagtg atggttcgct
     6781 tcatctttta gaaagggtaa ccggggaaga ggtcattgtt gatgttgttt tccccatagt
     6841 gcatggaaca tatggtgagg atggcacctt gcagggactt ctggagatgg ttgatgtgcc
     6901 atatgttgga ccgggtgtat tgggttctgc tgttggcatg gataaggata ttgcaaaaag
     6961 agtgttaatg aatgcaaagg gatttgcgat agttcctttt aaaacaatcc gcatcaatga
     7021 aatgaatcct cacaataaac aaacaatact gaggatggtt gagaggatgt tacccgtatt
     7081 tataaaacct gcaaatgctg gttcatcagt tggtatatca aaagttacca gcaaaggtat
     7141 gattgaagag tcactatcgc ttgcttttca atatgatacc aaggtgatag ttgaaaaggc
     7201 tattgactgc cgtgaatttg aagtgtcggt gatggggaat gaagaacctg ttgcatcggt
     7261 tgttggggag attataccac agcatgaata ttattcatac aaagcaaaat atcttgaaaa
     7321 ggatggcgca acattagcta taccggcaac cataagtaaa agtttggaac ataatttaca
     7381 aaaagcagcc tgtatcatct tttcattatt agaatgcgaa gggctcgcac gggttgattt
     7441 ttttgtagaa aagcaaacaa atattctata ctttaacgag ataaatacat tacctggatt
     7501 taccagtatc agtatgtatc caaagctgtg ggattgctca ggaatcccct ataccaaact
     7561 cattgatacg cttatccatt atgcactaaa gcgtcaccgc agaaggaaaa agatggtggg
     7621 agcattcctc aggttaaaaa aataatcatg cataaccact aatgccatga actatcgcac
     7681 aaaaaaaata aaaggacttt atgtcgatac tgtgttattt gatatggatg gtgtgattgt
     7741 tgattccatg tatgcgcacg cccggtgctg gatacaggta tttgaagaac atggcgttca
     7801 tctggacgca attgatattt taaaacgtga aggcatgtcg ggacttgaat cgatagctga
     7861 tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgaccac aatgaactga
     7981 tagttttttt aatgaactgg tgctcctcgt gaagtgtgtt ttttcattga caggtgtgta
     8041 aatataccta tagttgcgtt aatggtgttt acagatgtta aaaatttata aataataact
     8101 gtgggtgtaa tatgagtgtt gcatggattc ctaacacatt gacactgggc aatttagttt
     8161 taggctttat tgcaataatt tttgcaagca tgaacattcc tcatcatcta tctattgcag
     8221 gcgtattgat tttaggtgct gctttactgg atggattaga cggacaggta gcacggatgc
     8281 tgggtgttgc aagcaagtta ggacaggaat tggattcact ggcagattgt gttacatttg
     8341 gcatcgctcc aggatattta gcatttaaag gctatctaca gggaacatgg atttatatcc
     8401 ttgataaacc tattgatatt ggtatcttca ttgcagcaat tttccctgta tgtgcagcat
     8461 acaggctggc gcgttttaat gtgcagcatg tccccaacgc ttttgatgga ttgccatctc
     8521 ctatagcagg tattattgtg gcgttagtgc cattatgttt tataaatatt attattccca
     8581 aacttttatt tatcattggt tttgttcttg ttgctctgct catggtatcg acagtgcgat
     8641 atgcaaagcc tcagtcgttt atattgcaga agattacggg tataaaatta ttattgttta
     8701 tattaatctt aattgcgctc atttttatgt ttaaacaatg ggtgataatg gttatcattg
     8761 cgctttacat cttatcagga ctattaagtt ttattattca attgatacag gattaccgat
     8821 attaggctgt cttgaagtgc aatggattat taaaggataa gtgacggttg tcatagtttc
     8881 cctcgctttg cgaaggagag attgggggtg aggttaataa tcatacaaaa ttatataatt
     8941 tgttattttg cagcatcaat cgtaggcgat gtcccttctt ttctaaggta ttttaagtaa
     9001 tgattgcgct tttccattga atgaagcggt ttataccata aaccaatccc atagtaacta
     9061 tgtggcaagt gatttttaca gataattttt gaaaaatttt attaaaatcc tcaaaaattt
     9121 ttgtttccga tgatttaata tcatattttt aaataaaaag ctataaatgc tggtaattat
     9181 ttttagataa ttatatttaa tattggtaga aaaatatttt tttgctatat taatggttgt
     9241 tagcactctt tttattgagt gctaacacaa tttttttatc ttttattcca ataataaaat
     9301 tattgtaaaa aagaatgatt gtttattttt taagtatcta attattacat taaaatcaag
     9361 gataaggagg aacactgtgg gaatcaaacc attaggagat cgagttctca ttgaagtgac
     9421 ggaagaagaa cttccaaaac aggggagtct ttacatccct gatacagcaa aggaaaagcc
     9481 acagcagggg aaggtgctgg ctgttggtaa aggtcgttat gaggatggaa aatgggttcc
     9541 attggatgtt aaaacgggtg atactgtatt gtttggtaag tatgcaggta cagaggttaa
     9601 gtatggtggc aaggaatatt tgattgttcg cgaaaatgat attttagcta tcctggatta
     9661 aattacaaaa tcgtagaaaa ggaaggtgaa agacatggca aagattttac aatacaatga
     9721 tgaagcaagg cgatcaatct atcagggtgt aataaaatta tcagatgcag taaaagtaac
     9781 tcttggtccc cgtggtagaa atgttgtcat cgataaaaaa tttggctcac caactatcac
     9841 caaagatggt gtaacagtgg caaaagaaat tgagctggaa gatccatttg aaaatatggg
     9901 tgctcagtta gtaaaagaag ttgctacaaa gaccaatgat gtggcgggtg atggcactac
     9961 aacagcaact attcttgcag ctgctatcta tcatgaatca ttaaaaaatg ttactgctgg
    10021 cgcaaatccc atgagtttaa aacggggcat tgataaggct gtagaagtag cagtaaaatt
    10081 tattggcgat acagctcgtg aaattaagga taaaaaagag atcgctcagg ttgcagctat
    10141 ttctgctaat aatgacatgg aaataggcga gcttattgct gaggcaatgg ataaagttgg
    10201 taaagatggt gttatcaccg ttgaagaagc taaatctatt gaaacacatc tagaagttgt
    10261 tgaaggtatg caatttgata ggggttatat ttctccctat atggtaaccg accctgagaa
    10321 tatggaagca gtactggagg atgcatttat ccttatcaat gaaaagaaga tttctacaat
    10381 gaaagatctt ctccctatac tggaaaaagt tgcacaggct ggcagacccc ttcttatcat
    10441 tgctgaagat gtagaaggtg aagctcttgc tacaattgtt gtgaacacat tgcgcaaggt
    10501 tatacagtgc gtagcagtaa aagcccctgg atttggtgac cgcagaaagg caatgctgga
    10561 agatattgct atccttacca aagggcaggt catatctgaa gatctgggat taaagcttga
    10621 aaataccacg atggatatgc ttggacgtgc aaaaaaagtt attattgaca aggaaaatac
    10681 aacaataatt gaaggtgctg gcagccaggc ggatgtacag gcacggatca aagttcttaa
    10741 gaagcagata gaagaaacaa catcagatta tgaccgagaa aaattacagg agcgtttggc
    10801 aaaattagct ggtggtgtgg cggttatcaa tgttggtgca gcaactgagg ttgaacttaa
    10861 agaaaagaag gctcgcgtgg aagatgcact ttcagctact cgtgcagcag tagaagaggg
    10921 aattgtcccg ggtggtggtt taacgctgtt atatgctcag aaagctgttg cagaattaga
    10981 gaaaaaactg gatggtgatg aaaaaatagg tgtacggatt gttgccaaag ctatggaaga
    11041 gcctatgaag atgattgcat ttaacgctgg tgttgaagga tcggttgttg ttgaaaaggc
    11101 aaaacaggaa aaacagggta taggctttaa tgcagcaaca ctggagtggg aggatctcac
    11161 aaaagcaggt attattgatc ctgccaaggt tgtacgttca gcattacaaa atgctgcatc
    11221 agttgctggc atgct
//
LOCUS       DAOSOX010000076        11130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_5841_length_11130_cov_8.689571, whole genome shotgun sequence.
ACCESSION   DAOSOX010000076 DAOSOX010000000
VERSION     DAOSOX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5841_length_11130_cov_8.689571"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..367
                     /locus_tag="PLX22_06860"
     CDS             194..367
                     /locus_tag="PLX22_06860"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HOT19657.1"
                     /translation="MLQLVDRIGRLESQLNKNSHNSSKPPSSDGLKKKARKWSQRKRT
                     GKKPAGSLRHLMR"
     gene            440..550
                     /locus_tag="PLX22_06865"
                     /pseudo
     CDS             440..550
                     /locus_tag="PLX22_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(780..1772)
                     /locus_tag="PLX22_06870"
     CDS             complement(780..1772)
                     /locus_tag="PLX22_06870"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOT19658.1"
                     /translation="MTELFMRVLLKNLHTPGRHCASTGLRNLAAFHGLGWSEAFCFGI
                     GAGLGIWYIKSNNPPRMIHVRSADIEERFFLHIGMPVTWETYETQQQSHRELIKKLDA
                     GLPAIVQTDLYYLPYYKSSTHFPGHVITVWGYDDDKKVFYITDTERPALIEVAYSDLA
                     QARFCEDNFFKIHGNCIAPAMMKAPVNMQDTIRNAIIYNSRVILDTSISTQGIQGLTT
                     FYNELELWTQFEDWKWAFRFAYQVIERRGTGGGGFRLIYADFLKEATAYLPDIESLQL
                     PYYMQQTAAAWTAFAMALKEASEKEKPEISPVTTCLENVIQAETRYHTKALMLL"
     gene            1990..2763
                     /locus_tag="PLX22_06875"
     CDS             1990..2763
                     /locus_tag="PLX22_06875"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT19659.1"
                     /translation="MQSKKMVITVPEKARAYFKKGSDNLGILLLHGFTGTPAEMRYIA
                     EKLHNAGVTVYVPRYPGHGTSLVEMANTSVHHWFTAAREALFELKSHCREVYVAGLSM
                     GGIFAILLAKEFDIKKIALMSVPCTLKEKTIYITPLVGRFKKIIWIPNKTKGVCDERA
                     RKEHICYDDGIPVIQSWQLFKTIKKAMQVLPDVAAQSLIIQSKNDKVIPKHSAEYIYQ
                     HLGSEHKHLIWLTKSDHAITVDYEKDEVTHHCINFFLKQ"
     gene            2996..3808
                     /locus_tag="PLX22_06880"
     CDS             2996..3808
                     /locus_tag="PLX22_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT19660.1"
                     /translation="MSENNIILERKGSISIVTIDRPKRLHAFNDVMFDMLENVAARLG
                     ESLPRCIILTGSGDRSFSAGFDVQPDNPMVKRIVDAVSTKDIAPARETITRIRRAVDA
                     FVALPVPIIAALNGDAFGGGAELAIRCDLRVMARHAVICFSETKLGLMSDWGGGAALA
                     RLIGASRAADLLLTARKVLSDEALSMGLVNRVVDTGTALDEAIKIANLIAENGPHAVR
                     HVLALVRNSRNISYESSLEEEAERAIALIASGECFHGVSAFLEKRKPEFPDV"
     gene            4227..4787
                     /locus_tag="PLX22_06885"
     CDS             4227..4787
                     /locus_tag="PLX22_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT19661.1"
                     /translation="MELQQAIVERRSIRRFTDYYVTDEEIVQILEAARMAPSWANVQP
                     WEFIVVRDKSLIEKIVGTYSPTNPARKCSLASSALIVACAKTNVSGCKEGRDVTALAN
                     WYMFDLGMAVQNICLKAYELGLGTVVVGYFDHQQCNKLLQVPQDTTVVVTIPVGKPEV
                     SGKQGPPRKELMDCVYVNTYGNKFFK"
     gene            4798..5256
                     /locus_tag="PLX22_06890"
     CDS             4798..5256
                     /locus_tag="PLX22_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOT19662.1"
                     /translation="MDIKDKWIYFAAPLFTAAERQFNETLALYIEQAGFAVYLPQKEC
                     AKISKSAEIYTQCLKGLDNAWCVVAVVDGCDTDSGTSFEIGYAYAKGIPVIGVRTDFR
                     QNADDGHLNLMLSQSIAMLCFVNSLEDASIDTVARCVKEKINALLIKVLQ"
     gene            5291..5914
                     /locus_tag="PLX22_06895"
     CDS             5291..5914
                     /locus_tag="PLX22_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19663.1"
                     /translation="MKRVLSCIAWFTGGILAALVVLLIYGLAITQNAIIQDTHKQYYH
                     ATRLVIYGDVVIGFLSDSSSQNKNLETSPHSFKVMLAGTIAQITFRPFESVYVPPLYL
                     GTYHINASGHDGILVLQVKDGLLYGAVRFPDWANGVWEPLKGVVIKNNTLYFTRSVTN
                     EKERLKTGAREYFTQYYVGTYKENGKKIVGFYKSRGAKYLFEAYRKK"
     gene            complement(5917..6867)
                     /locus_tag="PLX22_06900"
     CDS             complement(5917..6867)
                     /locus_tag="PLX22_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT19664.1"
                     /translation="MNSLDTLLSQVTIFQRLSNEELSQLAPYFTRNNIGKDDVIFHEG
                     TYGNELFIVADGVIAAFIKLSDNSLREVARFSKGDFFGDMAIFENDVRSATCIAQTNC
                     TLLSLTADDFFAIMNNHPPIAIKIMYDMLTVISQRLLKTGSFLTEMIQWGEAARKRSI
                     IDEFTGVYNRSYFEDTIALLITQEKPFCLIMIDLDYFRKINEQFDHQVGDRAILAIVN
                     VLKKIFKESDAICRYGGDEFAIIVNGNNIDEAFNFAEQARVRVHHINCHELGMPNFTL
                     SISMGIAAFPQCGKDIIRTKADEALYMAKERGRNCVVIAS"
     gene            complement(6864..7790)
                     /locus_tag="PLX22_06905"
     CDS             complement(6864..7790)
                     /locus_tag="PLX22_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT19665.1"
                     /translation="MDTKAIVKGVPLFSSLDDSEIQIIEKYCAHKELSRQEKLFSFDE
                     SFNLLCIVTSGSIGIYSNGAQNRLIARYIPNESFGELNLCGISLGKVEAIALDDAGVL
                     LFPATGTFVSFVTDHADIGAKIIYKLLSNISYRIRETNKLISQNYGWVSELRKAIYTD
                     RLTGLYNRQYCEELFRENKEYVIAIVKPDKFKMINDTCGHKAGDVTLVAIAQSLRRAL
                     PFATHIRYRGDEFMIVIAGDADMKKTAAAISEAIKAIDIHAITDGAINVITASIGISK
                     NTITQQAIENTYTIMWSAYNNGGNSIAMEGTK"
     gene            complement(7999..8238)
                     /locus_tag="PLX22_06910"
     CDS             complement(7999..8238)
                     /locus_tag="PLX22_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19666.1"
                     /translation="MAGKKASNTTTIKTTKRQSTEKKSKTNSIPIESNRIHEAIRIQA
                     FYNYLHRIMNNLPGDQLSDWLEAEKYIKKTINKQQ"
     gene            8641..9510
                     /locus_tag="PLX22_06915"
     CDS             8641..9510
                     /locus_tag="PLX22_06915"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT19667.1"
                     /translation="MKKIAINLHPQDEKSLKLVAAFIKISHAKGFNVVLPDYALLHDD
                     FADYIANHEDYINADVAIAIGGDGTFIRTARMFAAGSVPILGVNRGRLGFLTEFLPQE
                     ALDFFDELASNTYTVSQRKMLKANIIRNQHQFAAIDFLNDAVITKGSFSRPIRIRLEL
                     NNEFVNCYAGDGLIIATATGSTAYSLSAGGPIVLPDDESVFVVNPVCPHTLAIRPMVV
                     PDSMVLSARVVTSIENLLLTIDGQEAIALQDTDVIHFSRSVHVIQVINHPSRRYFDVL
                     RQKLGWGANNEIE"
     gene            9514..>9923
                     /locus_tag="PLX22_06920"
     CDS             9514..>9923
                     /locus_tag="PLX22_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT19668.1"
                     /translation="MLVELRIKNFILIDELSISFGKGLNILTGETGAGKSILIDALSG
                     VLGEKMSTDMIRSGYDRAVLEGVFDIGSLPQVKQLLELSGIDNDDDTLILRREIYANG
                     KGRCFANGVQIPLAKLKEIADYLVDIHGQNEHQN"
     assembly_gap    9924..10023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10024..10953
                     /locus_tag="PLX22_06925"
     CDS             <10024..10953
                     /locus_tag="PLX22_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT19669.1"
                     /translation="PNLSPLLESVRDAYFSLEDTHATLRDYARSIDFSPERINQVEER
                     LALIASLKKKYGDTIEDILAYAQNAKRELATITSTEEALDKLQQEYDSTLTKAREVAL
                     LLSDKRLEAAKKLEGMVTSELADLGMQGTVFRVSIKRELSPDGQIEANNKRYILYPHG
                     LDRVEFLLSANEGEDLRQLRKAASGGEMSRIMLALKKCILDADIVDSLVFDEVDAGIG
                     GKTAEVVGKKLKTLAKQRQVLVITHLPQIAAMSDNHYMVQKKKVNARTTTLVEKLSDD
                     KKVHEVARMLAGEVVTELSVKHAKELIELASQQ"
BASE COUNT         3278 a         2018 c         2403 g         3331 t
ORIGIN      
        1 gcaagcaatt tattttttta taaattacta tctatgaggc cattaaatat atattgtaac
       61 tactcagcta aaatatttca cgtagaaaaa aattttgcta ttaatcgtag aggcatggta
      121 tagattatgt ttcatgaaat gagaagaagc tgaaaagata taggtaccga gaaaacatgc
      181 agttggtgat tgtattcttc aattagttga caggatagga agacttgaaa gtcaactcaa
      241 taagaacagt cataacagca gcaaaccgcc atcaagtgat ggattgaaga aaaaggcaag
      301 gaagtggagt caaaggaagc gaacggggaa aaaaccagcg ggcagtctga ggcatctgat
      361 gcgataggca tagtaccatc ttaccaacat tgaaaggagc atctgcactc cgcaaaagta
      421 ttggagttat aatgttgcta ttccatttgg taacaatctt tcggaaagag atatccgtat
      481 ggttaaagta caacagaaaa tttcaggact attcagaacg tttagtaatg ctgaacaatt
      541 ttgtattatt tcgaagttac atcccgacag taaaaaaagc agtgttttag tgttattgat
      601 gcactcgaac gcatacttaa tggtaagcag gtttatcgtg agtttgtttc ttaatagctg
      661 agtagttaaa attaatgttt atggatgctc cattgtaatg aataaaaaat tgctttaaag
      721 aaaactctat ggttatatat agttccagtt acatgctttt ttataaatga taataactat
      781 cacaatagca ttaaggcttt tgtatgatac cgtgtttctg cttgtataac attttcaagg
      841 cacgttgtaa ccggactaat ttcgggcttt tctttttctg atgcttcctt caatgccatt
      901 gcaaaggcag tccatgctgc agcagtttgt tgcatatagt aaggtaattg caatgattct
      961 atgtctggca aataagcggt agcttcttta agaaaatcgg catatatcag tcggaaacct
     1021 ccgccacctg ttccacgcct ttcaatgacc tggtatgcaa agcgaaatgc ccacttccag
     1081 tcttcaaatt gagtccatag ttcaagctcg ttataaaagg ttgtaagccc ctgaataccc
     1141 tgtgtggata ttgatgtatc aagtataaca cggctattgt agataattgc attacggata
     1201 gtgtcttgca tatttactgg agctttcatc atggcaggtg ctatgcaatt gccatggatt
     1261 ttaaaaaaat tgtcttcgca gaaacgtgcc tgtgcaaggt cgctatatgc aacttcaatt
     1321 aatgcagggc gttcagtatc ggtgatgtaa aacacctttt tatcatcatc atagccccag
     1381 acagtaatga catgcccggg gaaatgagtg gatgacttat agtaaggaag atagtataaa
     1441 tcggtttgta ctattgcagg taaaccagcg tcaagttttt taataagttc acgatgactt
     1501 tgctgttgtg tttcatatgt ttcccatgta acaggcatgc caatatgcaa aaaaaaccgt
     1561 tcttcaatat ctgctgaacg tacatgaatc atgcgcgggg gattattgct ttttatatac
     1621 catattccca aacctgcacc aatgccaaaa cagaaagcct cactccaccc caatccatga
     1681 aatgcagcaa ggtttcgtaa tcctgtactg gcacagtgtc tgccgggtgt atggagattt
     1741 ttaagtaata cgcgcataaa taattcagtc attatagttt gtcatggttc ttgtacctat
     1801 attattctgt ttaaagaata atgtcaactg taatatagca ggtaaaagct atagttgaca
     1861 taacaccatg catggtgttt aattattgtg tttaatatga agaataaaat aaataggaga
     1921 gatagttatt tgattaatta ttttctttat attaatgttt tttatagttt gtgaaaagga
     1981 ggagttatta tgcaatcaaa aaaaatggta attacagttc ctgaaaaagc cagagcatat
     2041 tttaaaaaag gatcagataa tctgggtatt ttgctccttc acggatttac cggtacccct
     2101 gctgagatgc gatacatcgc agagaagctt cacaatgcag gtgtaacagt ctatgtacca
     2161 cggtatcctg gtcatggcac cagtttggtt gagatggcaa ataccagcgt tcaccattgg
     2221 tttacagcag cacgtgaagc attgtttgaa ttaaagtcac attgtcggga ggtctacgtc
     2281 gcagggcttt ctatgggtgg tatttttgca attttgctgg caaaagaatt tgatattaaa
     2341 aaaattgctc ttatgtcggt accctgcaca cttaaagaaa aaactattta cataacgcca
     2401 ttggttggac gttttaaaaa gattatatgg attcccaaca aaactaaagg tgtgtgcgat
     2461 gaacgggcac gcaaagaaca tatctgttat gatgatggta tcccggtcat ccaatcgtgg
     2521 cagcttttta aaaccataaa aaaagctatg caggtactgc ctgatgttgc tgcacaatcg
     2581 ctcattatac aatcaaaaaa tgataaagtt atccccaaac attcggctga gtatatctat
     2641 caacatctgg ggtcagagca caaacatctg atatggctta caaagagtga tcatgcaata
     2701 actgttgatt atgaaaagga tgaagttaca catcactgca tcaatttttt tttaaagcag
     2761 tgacctgttc atgtgatttc tttgtgtaca tcaatgcgca ggctttgggt tatatttttt
     2821 tatgagcatg aataatcttt ttgacaaata tgggagatag ttattcatta acaagcaaca
     2881 tctgttaacc ctgacaccgg taagctcgtg agtaaagggt aacctctgta ttcgccagga
     2941 gagctgtttc gtaatgatgc gcaagtgtca ggtccttatg tgtgaggagg gtacaatgtc
     3001 agagaacaat attatccttg aacgaaaggg ttcaatttca atagtaacga tcgacaggcc
     3061 aaagcgcctc catgctttta acgatgtgat gttcgacatg cttgagaacg ttgccgccag
     3121 gcttggcgaa tcattgccgc gatgcatcat actaacggga agcggagacc gctctttcag
     3181 cgcaggattt gacgtacaac ctgacaatcc catggtgaaa agaattgtgg atgcggttag
     3241 cacaaaggac atagcgcccg ctcgggaaac aataacccgt ataagaaggg cagtcgatgc
     3301 gtttgttgcc ctgccggttc ccattatcgc ggcattgaac ggcgatgctt tcggaggagg
     3361 ggcagagctt gccatcaggt gcgatctgag ggtaatggcg cggcacgccg ttatctgttt
     3421 ttcggaaaca aagcttggcc tcatgtccga ttggggagga ggtgcagccc tggcaaggct
     3481 tattggtgct tcaagagcgg cagatttgct tctgactgca cggaaggtgc tatccgatga
     3541 agcactttca atgggattgg ttaaccgtgt cgtcgatacc ggtactgcgc ttgatgaggc
     3601 tatcaaaatc gccaatctta tcgccgagaa tggcccccat gcggtacgtc atgtgctggc
     3661 gcttgtccga aacagcagaa atatctcata cgaaagctct ctggaagagg aagccgaacg
     3721 ggcaattgct ctcattgcct ctggtgaatg cttccatggt gtctcagctt tcctggaaaa
     3781 gcggaagccg gaatttcccg atgtgtgatg agatgaaata gggacaataa taaataatag
     3841 aagtgtaata attctttgta tcaaatcaca ttcaaaaact tctctggtag tatctttaaa
     3901 ggagtgagaa gcgtgatgct catgatgatg aaaagcatgc tgtttttcag ttcttcaatg
     3961 tgtatgcatg atacgccgat aaagcaagcg ttctgtgttt atacatctga tatattgata
     4021 ttgtgcactc tatagccggt cgttttttga catttctttc tgtatttcct atggttgcat
     4081 tgtgcatgtt acctatccca caaatgttct tgtgtgattg tgcacaaata cattataata
     4141 ttcatttttg ctttacataa taatgcctat tttatactat caacattgat gcatttttat
     4201 attgtatgcc acatgaggag gtagctatgg aattacagca agctattgtt gaacgcagga
     4261 gcatcaggcg ttttactgat tattatgtta ctgatgaaga aattgtgcag atactggaag
     4321 ctgcacgcat ggcaccatcg tgggctaatg tacagccatg ggaatttatt gttgtacgtg
     4381 ataaatcatt aattgaaaag attgttggaa catattcgcc aaccaatcct gcacggaaat
     4441 gttcattggc gtcttcagcg ctcattgtgg catgtgctaa aacaaatgtt tcgggttgta
     4501 aagaaggcag ggatgttact gctctggcaa actggtatat gtttgattta ggtatggcgg
     4561 ttcaaaatat ctgcttaaaa gcttatgaac tgggattggg cacggtagtg gtgggttatt
     4621 tcgatcatca acaatgtaac aagctattac aggtgccaca ggataccacc gttgttgtaa
     4681 cgatacctgt tgggaaacca gaggtaagtg gtaagcaggg acctcctcga aaagagttga
     4741 tggattgtgt ttatgtgaat acctatggaa ataaattttt taaataagta aaacgatatg
     4801 gatataaaag acaaatggat ctattttgct gcaccacttt ttacagcggc cgagaggcag
     4861 tttaatgaaa cactggcatt gtacattgag caggctggtt ttgcggtgta ccttccccaa
     4921 aaagaatgtg ccaaaatcag caaatctgcg gagatatata cgcagtgctt aaaaggactt
     4981 gataatgcat ggtgtgttgt tgcagtagtg gatgggtgtg atacggattc cggtacaagc
     5041 tttgaaatag ggtatgccta tgcaaagggg atacctgtta ttggcgtcag aactgatttc
     5101 agacaaaacg ctgatgacgg gcaccttaat ctgatgctgt cgcaaagcat tgctatgttg
     5161 tgttttgtca acagccttga ggatgcatca attgatactg ttgcacgatg tgttaaagaa
     5221 aagattaatg ctctgctcat caaggtgtta caataaaatt aaaataaaca tggaataagg
     5281 gtggtttacc atgaaaagag tactatcatg tattgcatgg tttacggggg gaatacttgc
     5341 tgcacttgtt gtactgctta tctatggatt ggcgattact cagaatgcta tcatacagga
     5401 cactcacaaa caatactatc atgccacaag gcttgtcata tatggcgatg ttgttattgg
     5461 atttttaagc gatagttcct cgcaaaataa aaatttagaa acatcacccc attcattcaa
     5521 ggtgatgctt gccggaacta tcgcccagat tacgttccgt ccttttgaaa gcgtttatgt
     5581 tcctccactc tatttaggta cataccatat caacgcttcg gggcacgatg ggatattggt
     5641 gttgcaggtt aaggatggat tactctatgg ggcggttcgc ttccctgatt gggcaaatgg
     5701 tgtatgggag ccattaaagg gtgttgtaat aaaaaacaat acactctatt ttacacgttc
     5761 agtgacaaat gaaaaagaaa ggttaaaaac cggcgcacga gaatatttca cacaatacta
     5821 tgttggtacc tataaagaaa atggtaaaaa gatagtgggc ttttataaat cacgtggtgc
     5881 aaaatatctt tttgaggctt accggaaaaa gtaatattac gaagcaataa ccacacagtt
     5941 tctgccacgc tcttttgcca tgtagagcgc ttcatctgct tttgtacgta tgatgtcttt
     6001 gccacattgc gggaatgctg caatccccat gctgatggaa agcgtaaagt tgggcattcc
     6061 caattcatgg caatttatat gatgaacacg tactctggct tgttcagcaa aattgaatgc
     6121 ttcatcaata ttgttgccat tgacaatgat agcaaattca tcgccaccgt agcggcatat
     6181 tgcatcggat tctttaaata tttttttaag cacattgact atagcaagta ttgcgcggtc
     6241 gccaacttga tgatcaaact gttcattgat ctttcggaaa taatcaaggt caatcataat
     6301 gaggcagaat ggtttttcct gtgttatcaa aagtgcaata gtatcttcaa aataactgcg
     6361 gttgtaaaca ccggtaaact catcaataat tgaacgctta cgtgctgcct cgccccattg
     6421 aatcatttcg gttaaaaacg atccagtctt taaaaggcgc tgtgagataa ccgtgagcat
     6481 atcgtacatg atcttaatag caatgggtgg atgattgttc attatggcaa agaagtcatc
     6541 tgcggtaagc gaaagcaggg tgcagttggt ttgtgcaata caggttgctg aacgtacatc
     6601 gttttcaaag attgccatgt caccaaaaaa atctccttta ctgaaacggg caacctcacg
     6661 cagagaatta tcggacagct ttataaatgc agcaatgaca ccatcagcta caatgaaaag
     6721 ctcattgcca tacgtccctt catgaaagat gacatcgtcc ttaccaatat tatttctggt
     6781 aaaatagggt gccagttgag ataactcttc attgctgagc ctttgaaaaa tagttacctg
     6841 tgacagtaaa gtatccaggc tgttcacttt gtaccttcca ttgctattga attgccacca
     6901 ttgttatatg ctgaccacat tattgtatac gtattttcaa tagcctgttg agtaatggta
     6961 tttttactta ttccaatact tgccgtaatg acattgatgg ctccatccgt tattgcatgt
     7021 atatcgatgg ctttgatggc ttctgaaatt gctgcagcag tttttttcat gtcagcatca
     7081 cctgctatca caatcataaa ctcatcaccc ctgtagcgta tgtgtgtggc aaacggtaga
     7141 gctctgcgca gggattgtgc tattgccaca agcgtgacat cacctgcttt gtgtccgcag
     7201 gtgtcattta tcatcttaaa tttatcaggc tttacgatgg caatgacata ttctttattt
     7261 tccctgaata gctcttcgca gtactgtcgg ttatacaggc cggtaagcct gtcggtgtag
     7321 atagcctttc gcaattcact tacccagccg taattttgtg aaataagttt attggtttcg
     7381 cgtatcctgt atgatatgtt cgatagtaac ttataaatga tctttgcacc aatatcagca
     7441 tgatcggtta caaatgatac aaaggtgcca gttgccggaa acagaagcac accggcatca
     7501 tcaagggcaa tggcttcaac cttccccagc gatatgccgc atagattaag ttcgccgaaa
     7561 ctttcattgg gtatataccg ggcaattaat ctgttttgtg ctccgtttga ataaatgcct
     7621 atgcttcctg aagtgactat gcacaacagg ttgaatgatt catcaaatga aaaaagtttt
     7681 tcctgccttg ataattcttt atgtgcacaa tatttttcaa taatctggat ttcgctgtcg
     7741 tcaagcgatg aaaatagcgg cacacctttt actattgctt ttgtatccat tataccttct
     7801 gttgacttct tctatactgt agtgaacact ttttcatcat gtacctttga atgcaataaa
     7861 aaaataatca gtaactatcg ccagaagcat ataaaaataa aattgctccc ttgcaacgtt
     7921 gcatttttta aaaggcatgt actgggtata gagtattatt tattttcagt gcagatgcaa
     7981 ggtcaaaaaa agatgcatct attgctgttt attgatggtc ttttttatat atttttcagc
     8041 ttccaaccaa tccgatagct gatcacccgg taaattattc atgatacgat gtaaatagtt
     8101 atagaatgcc tgtattcgta tggcttcatg aatacggtta ctttcaatcg gaattgaatt
     8161 ggtttttgat tttttttcag tgctttgacg ctttgtggtt ttaatggtag ttgtattgct
     8221 tgcttttttc ccggccataa ggatacctcc ttcccaaatt gtgtattgaa tagtttatac
     8281 taagggaata aatagtataa aaaaataata ttgtcaatca attttataga aaataaacaa
     8341 aactccattg ccataatttc attgacataa tctggttaac cggtgtagta ataactcatc
     8401 ataagtaaat gaacggacag cttttgataa cgattgtgtc ggcatccata tatgatgaaa
     8461 atagctggag gcagtcattc tacatcatgg ttcagaatat aacgttgtat atcagaattc
     8521 cggaacagat ccggaatgac agaggtggca taatgctatt ttctattttc aaagcagtta
     8581 actcaatagt aagtatcata tttttactct tataaagcta atagtggtaa aaggaaaaca
     8641 atgaaaaaaa tagctataaa tttacacccc caggatgaga aatcactgaa gcttgttgca
     8701 gcattcatta aaatttcaca tgcaaaaggc ttcaatgttg ttttacctga ttatgcgttg
     8761 ttgcatgatg attttgctga ctacattgca aaccatgaag attatatcaa cgctgatgta
     8821 gcaatagcca ttggtggcga tggtaccttt atacggacgg ctcgcatgtt tgctgctgga
     8881 agcgttccta ttctgggcgt caatagagga cgattgggat ttttaaccga atttcttcct
     8941 caggaagcat tggatttctt tgatgaatta gcgagcaata cctatactgt ttcgcaaaga
     9001 aaaatgctga aggctaatat aatccgcaat cagcatcagt ttgccgcaat agatttttta
     9061 aatgatgcgg ttatcacaaa aggttccttt tcacgaccaa tcaggatacg gctggagcta
     9121 aacaatgagt ttgttaactg ctatgccggt gatggtctta tcatagctac tgcaacgggt
     9181 tctacggcat attcgctttc ggctggcggg ccgatagttc ttcctgatga tgaatccgta
     9241 tttgttgtca atcccgtatg cccgcatacc cttgccatac gtcccatggt tgttcctgat
     9301 tcaatggtac tatctgccag ggtggttacc agcattgaaa atttattatt gacaatagat
     9361 ggtcaggaag ctattgcttt gcaggatacc gatgtgattc atttttcacg gtcggtacat
     9421 gtgatacagg tgatcaacca cccatcaagg cgttattttg atgtattgcg tcaaaaatta
     9481 ggatggggtg ctaataatga aatagagtga actatgctgg tagaattacg aattaaaaat
     9541 tttattttga tcgatgagct atcaatcagc tttgggaagg gattaaacat cttaaccggc
     9601 gaaacgggtg cgggtaaatc catccttatt gatgcgctct caggagtgct gggcgaaaag
     9661 atgtcaaccg atatgatacg ctcaggatat gacagggcag tgctggaggg cgtttttgac
     9721 ataggttcac tgccgcaggt aaaacaattg ctggaactat cgggtataga taatgacgat
     9781 gatacgttga ttttacgtcg tgaaatatat gccaatggca agggacgatg ttttgccaat
     9841 ggtgtgcaga ttccgcttgc aaaattaaaa gagatagctg actatttagt agatattcat
     9901 ggccagaacg agcatcagaa tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnncctaatt tgtcaccgct gcttgaatcg gtgcgcgatg cctatttttc acttgaggat
    10081 actcatgcta cattgcgtga ttatgcccgt tctattgatt tttcacctga acgcatcaac
    10141 caggttgaag aacgtcttgc gctcatcgca agcttaaaga aaaaatatgg tgataccatt
    10201 gaggatatat tggcatacgc acaaaacgca aagagggaac ttgcaacaat aacatccacc
    10261 gaggaagcgc ttgataaatt gcagcaggag tatgatagca cattaacaaa ggcacgcgag
    10321 gtagcgctgt tgctttcgga taagcgcctt gaagctgcta aaaaattaga agggatggta
    10381 acatctgagc tggctgatct gggaatgcag ggcactgttt tcagggtttc cattaagcgc
    10441 gagttaagtc ctgatggaca gatcgaagca aacaataaac ggtatatttt atatccgcat
    10501 ggtcttgaca gggtagagtt tttgctgtcg gcaaacgaag gcgaggattt gcggcaactg
    10561 aggaaagcag ccagcggtgg tgagatgtcg cgcatcatgc tggcgctgaa aaaatgtatc
    10621 cttgatgctg atattgttga ctcgctggtg tttgatgagg ttgatgcagg cattggaggc
    10681 aaaacagcgg aggttgttgg gaaaaagttg aaaacattag ctaaacagcg gcaggtgctt
    10741 gttataacgc atctccctca gatagcagca atgtccgata atcactatat ggtgcagaag
    10801 aagaaagtca atgcacgaac aacaacactg gtagaaaagc tttctgacga taaaaaagtt
    10861 catgaggttg cccgcatgtt agctggtgag gtagttacag aactatcggt taaacatgca
    10921 aaagaattaa ttgaactggc atcacagcag tgatatgaaa agcgatagtt ctatcaacct
    10981 ttctgatttt atgacatgcc tgctggttga tatcatacaa tcaacgccca cgtaccagca
    11041 tattgacatg aatcgtattc ttgtttgcag cgcttacaat agaaacagca ggggcggtgc
    11101 aatatttgcc aaggttgtac ccctgcgatt
//
LOCUS       DAOSOX010000077        10865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6030_length_10865_cov_8.470028, whole genome shotgun sequence.
ACCESSION   DAOSOX010000077 DAOSOX010000000
VERSION     DAOSOX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6030_length_10865_cov_8.470028"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLX22_06930"
     CDS             complement(<1..575)
                     /locus_tag="PLX22_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT19670.1"
                     /translation="MKHYKVTLAIPDTVAQEINTICTILLDNGFECYLVGGSVRDLIL
                     QIPVYDYDFATNARPEQVMKLFKKVVPTGIKHGTVTVLMSHAHYEITTYRSDGKYTDG
                     RHPDSIAFSDSLKEDVERRDFTINGLAYDVNKKVIIDYVGGMKDIKKKVIQTIGDPVQ
                     RFSEDGLRPYRACRLAAKLHFSINKKTLEAIP"
     gene            complement(572..2800)
                     /locus_tag="PLX22_06935"
     CDS             complement(572..2800)
                     /locus_tag="PLX22_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT19671.1"
                     /translation="MKIFPIHELIYTVQKFPLLQSMTPCEIVRSLNFFEDVPFDCLNE
                     IVHNINIHQFNDNEIVARHGRYNEWFYIVLSGKVSVFMITPDYTKSELYSIEPDNFFG
                     EDIVISNEPRESTAIACGESILLAVDAQSIKKIIASSTRTNEMLNNAFLQRKMRNNLR
                     SIPIFTHLNDEIFNEIVDVVNVVQKKKGDVIFKQGDTGDALFMIRKGDVSVYRKMNKK
                     DELISLLAEGNFFGEMALVLGEPRNATVIANNDCELLKIEKKDFDTIVLRHKDLYNSI
                     HTVALERVSGHELVNATEAIVSKKLIELNRKLNKHIDVIAQCTFETPNGSALLATLPG
                     SRYPYVYPRDSACATRMLYRVAMSTLRSNDIAFRLLAGIARFICNCQRSDGYWGQRYG
                     LDTTEKSIYKQEDNVAHGVTILCRYCLAAKHKGHAPGDSEAYINAIYNGAMFAIKRYY
                     RNEIHLFYSTTSIHESAIEEGYSIWVNYAYWCMLSLIEKVAQEYDCVSRFERVIDLKE
                     SFGTIVNNVFTIDDRLIRRLKPDGEADLRPDITLMSPFFFGTGVMPHVFTEDAIFSNT
                     MKFVEDTLWDPDLGMLQRYLPFIEDPNTHVHAGNGPWVQYTAMLAQYNYFTGKVEKGD
                     RIIDSIDRYSADGYLCEHLTTPERFREFLTLEWLSGTDVSKEFAANIMVDGITYDLIV
                     EELNHMKNSYEQIKKKIEAGADNYLTFAMPLMWSHAEYAMALLLKTWRELLDSGVKNN
                     LL"
     gene            complement(2892..4553)
                     /locus_tag="PLX22_06940"
     CDS             complement(2892..4553)
                     /locus_tag="PLX22_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19672.1"
                     /translation="MIKKTIQHYIGIIALVIGCCLVTGLSYEIAYTIIFTPDGLDLFN
                     AKKVRLKNNTAGYTVATINYKESVNPLITDLLLSFDSDFPYLAKDDSGHYAIRYAQYK
                     YLQDAIEGEGCAQFFHRDHRVDIQTAKNNWLTNCDDLGSFTIEASIKLLQYSSAAIVL
                     SRTGFSQGKQGFEMLCVKNKLAVRFYKMFYKKDNTPIDVFLHRGPSLPLNAWQHISIS
                     FDRISGKLETWINGDVVETVYCTESGEPFVDVLVPKFNCHDLPIATVGKNFYGCIDKV
                     RISQRYIENLQKETAIAMSHYKPVSYNNRDTINREGIITSPVYQFPDFGTMVLLFHWQ
                     EQLPPSTYVWMEFRISDEMFKSDDMLPQWYRINNNQKNIYLKKVGDSYCRGKYYQWRA
                     HLISSPDGTNAPTIYAIELQYALDKAPQPPVMVAVDSVSDRVITLKWKKNVEYDILGY
                     KIYYGVKSGKYDGVITTIGNKRITNELSDNNGYISVTLTNEIIEENRNNDAKGFLQYP
                     VLKNNVIYYFAVTAYDSYKPDTLFNHESKPSNEVSARPEPGSDIQ"
     gene            complement(4557..4988)
                     /gene="rpiB"
                     /locus_tag="PLX22_06945"
     CDS             complement(4557..4988)
                     /gene="rpiB"
                     /locus_tag="PLX22_06945"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT19673.1"
                     /translation="MKIGIGNDHAGVDLKYSIIKTIPEHIFINAGTDSYESCDYPDYI
                     KLVAEMLQREEVDCGIAICGTGIGASIVANKHRGVRAALCTNEYMATMARKHNDANML
                     VLGARVVGIDLAIAIINAFLHATFEGGRHQQRLDKIQQIEH"
     gene            complement(5202..6587)
                     /gene="asnS"
                     /locus_tag="PLX22_06950"
     CDS             complement(5202..6587)
                     /gene="asnS"
                     /locus_tag="PLX22_06950"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT19674.1"
                     /translation="MKRIRIVDCLQSDKSLESCTVAGWVRTKRESKENIFIEINDGSC
                     LTNMQVVVGFGDSCYETARQLHTGASVYITGKLVASPAAGQKWELKPDKLIVYGDADP
                     ESYPLQKKRHSFEFLREIAHLRPRTNTMGAVLRIRNQVSYAIHTFFQQKGFLYVHTPI
                     ITTSDCEGAGEMFQVTTLDLLHVPVQDGFIDYSQDFFGAKAALTVSGQLEGELLALAM
                     GDIYTFGPTFRAENSNTTRHLSEFWMVEPEMAFATLEDDCTLAEEFLKYIFTWVIDRC
                     HEDMVFFDERIKPGILHNLESIITSSFEIVTYTDAIAQLKQSPEPFEYAVKWGIDLQT
                     EHERWLTEKYYKKPIIVIDYPKEIKAFYMKRNDDNKTVKAMDVLVPGVGEIIGGSERE
                     DRYDVLLQRMQAMGLNPDDYWWYLDTRRYGSAPHAGFGLGFERAIQFITGMQNIRDVI
                     PFPRTPGSAKF"
     gene            complement(6727..6996)
                     /locus_tag="PLX22_06955"
     CDS             complement(6727..6996)
                     /locus_tag="PLX22_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19675.1"
                     /translation="MFRYLYGIHSGQRGCPDIRKLIIEPILPDKNAMLQPFVSPGLGF
                     EIDKKLLSKYGKRYFKMTELGLKIKVIREKGLKEALLLKKRKEGQ"
     gene            7308..8105
                     /locus_tag="PLX22_06960"
     CDS             7308..8105
                     /locus_tag="PLX22_06960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT19676.1"
                     /translation="MRLLYITLLLLFTVSTLHAQDTVCVLVYHTFMGKKIHYDISLEE
                     FHKQLLELKDHGFTFVTFHEIKNGLVKGNKNILVTIDDGHRTALDAYYSVLKPMRIKP
                     LLGINTFIIGKKPYVLTWDELKQLSREGCYIASHGYFHLFINDKLYKNNYRYFKLEVF
                     ESKKMLEEKLCTPVDTFIYPFGVVSDVGKKALIEAGYNYAFTIKWGNTSLPITSNNLE
                     ISRYMYNQNWNAIARAIIYKHKNPSTPHKESEKILVSTMPRLKGTHQ"
     gene            complement(8071..9468)
                     /gene="gltA"
                     /locus_tag="PLX22_06965"
     CDS             complement(8071..9468)
                     /gene="gltA"
                     /locus_tag="PLX22_06965"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT19677.1"
                     /translation="MADKIPRQKMPQQDAKNRIRNFNEVPLGYDEKTAMVEAARCLQC
                     KRPACVDGCPVNVNIPGFIKALKEGRPLEAFFTIKQTNSLPAVCGRVCPQEDQCEKLC
                     VLAKKGDAVAIGNLERYVADYEREKEHFVAQKSDSHTNKKVAIIGSGPAGLTAAGDLA
                     RIGYDVTIFEALHKAGGVLVYGIPEFRLPKDIVQYEIDALKAAGVTIGLSRVIGTAET
                     VNDLLSTGFDAVFVATGAGLPLFLDIPGENLQGVYSANEYLTRSNLMKAYKFPEYDTP
                     IVKGQKVAVFGGGNVAMDSARTALRLGSKEVYIVYRRSRTEMPARVEEVERAEEEGVI
                     FTLLTNPVQFIGDEKGRVKAVECLKMELGEPDASGRRSPIPVKGSEFTIAIDVAIIAI
                     GNGPNPLIPKTTPGMNITKRGNIIADPETMKTSKIGVFAGGDIVTGAATVIQAMGAGR
                     KAAKAIDEYLSTGAW"
     gene            complement(9458..10300)
                     /locus_tag="PLX22_06970"
     CDS             complement(9458..10300)
                     /locus_tag="PLX22_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT19678.1"
                     /translation="MYTIVTNEKISDNVGKAIVEAPYIAKHRKAGQFVIVIVDEVGER
                     IPLTIADADSATGTITLYYQIVGTSTHKLSLKKKGEELFNVAGPLGHPTDIQCYGTCV
                     CVGGGIGIAAVYPIAVAMKDAGNYLISIIGARNKNLLILENELKAKSDEMIVTTDDGS
                     HGVKGFVTDELKTILDGGKVDIVVAVGPVPMMKAVSDMTKQYGVKTLVSLNSIMVDGT
                     GMCGGCRVTVDGKKQFTCVDGPEFDAHLVDFDELMKRLNTYKEQEKESFEKHKCKLEQ
                     YYGR"
BASE COUNT         3236 a         2421 c         1817 g         3391 t
ORIGIN      
        1 gggattgctt ccagtgtttt tttattaatg ctgaaatgca acttggccgc taatctgcac
       61 gctctatatg gtctcagccc atcttcacta aaacgctgta ccgggtcacc aattgtctga
      121 ataacctttt ttttaatatc cttcataccg ccaacataat caataattac ttttttattg
      181 acatcatacg ccaacccatt gatagtaaaa tcacggcgct caacatcctc tttaaggcta
      241 tccgaaaatg caatactatc aggatgcctg ccatcggtat acttgccatc cgagcgatac
      301 gtggtaatct catagtgtgc atggctcatc aaaacagtga cggtaccatg ttttattcct
      361 gtagggacta cttttttaaa tagtttcatt acctgttcag gacgagcatt tgtggcaaaa
      421 tcataatcat acacaggaat ttgtaaaatc aaatccctca ctgaaccacc aaccagataa
      481 cactcaaatc cattatccag aagaatagta caaattgtat tgatctcctg tgctaccgta
      541 tcagggattg ctaatgttac tttatagtgt ttcatagcaa attgtttttt accccactat
      601 ctaaaagctc gcgccatgtc tttaacaaca gtgccatggc atactctgca tgtgaccaca
      661 taaggggcat ggcaaatgtc aggtaattat cagcaccagc ttcaatcttc ttttttatct
      721 gttcatacga atttttcata tggttaagtt cctcaacaat taaatcataa gtaatcccgt
      781 caaccataat attggctgca aattctttac taacatcagt cccggacagc cattccagag
      841 tcaaaaattc acggaatcgt tccggtgttg tcagatgttc acataggtat ccatcagcac
      901 tatacctatc tatactatca ataattctat cccctttttc aacttttccg gtgaaataat
      961 tatactgtgc cagcatcgct gtatattgta cccagggacc atttcccgca tgaacatggg
     1021 tattgggatc ctcaataaaa ggtaaataac gctgtaacat ccctaaatcc gggtcccaca
     1081 atgtatcctc aacaaacttc atagtatttg aaaaaattgc atcttcagta aaaacatggg
     1141 gcataacacc ggtaccaaag aaaaatggac tcataagggt tatatcagga cgcagatcag
     1201 cttctccatc aggtttcagc cgtctgataa gtctatcatc aatagtaaaa acattgttaa
     1261 ctattgtacc aaaactttct ttcaggtcaa taaccctttc aaaccgtgat acacagtcat
     1321 attcctgcgc caccttttct atcagtgaca gcatacacca gtaggcataa ttgacccata
     1381 tagaatatcc ttcttcaata gccgattcat gaattgaggt tgttgaatag aagagatgta
     1441 tctcattcct gtaataacgt tttatagcaa acatcgctcc attatagatg gcattgatat
     1501 aagcctcact atcaccgggt gcatgccctt tatgtttagc agccagacaa tatcggcaca
     1561 gtatggtaac accatgagca acattatctt cctgtttgta tatacttttt tcggttgtat
     1621 caagtccata acgctgcccc caatatccat cacttcgttg acagttgcaa ataaaacgag
     1681 caatcccggc cagtaatctg aatgcaatat cattagaacg cagtgtactc atagcaaccc
     1741 gatagagcat cctggttgcg caggcactat cgcggggata aacataggga tagcgtgagc
     1801 cgggtaatgt tgctaaaagt gcactcccat ttggtgtttc aaaggtacac tgtgctatga
     1861 catcaatatg cttattcagc ttccggttca attcaatgag tttttttgaa actattgcct
     1921 cggttgcatt aaccagttca tgtcctgaca ccctttcaag tgcaacagta tgaatactgt
     1981 tgtataaatc tttatggcgt aaaacaatgg tatcaaaatc ttttttttca atttttaata
     2041 attcgcaatc attattagcg atgacggtag catttcgtgg ttcacctaaa acaagtgcca
     2101 tttcaccaaa aaaattaccc tctgcaagca atgatatcag ttcatctttt ttattcattt
     2161 ttctgtatac gcttacatct ccttttcgta tcataaacag ggcatccccc gtatcaccct
     2221 gcttaaagat aacatcgcct ttttttttct gcacaacatt aacaacatct actatttcat
     2281 taaatatctc atcattgaga tgagtaaata taggtattga tcgcaggtta tttctcatct
     2341 ttctttgtaa aaatgcattg ttgagcattt catttgttct tgttgaagaa gctataattt
     2401 tttttatgga ttgtgcatca actgcaagca gtatgctttc tccacacgct atcgctgtgc
     2461 tttcacgtgg ttcattacta ataactatgt cctcaccaaa gaaattatca ggctctattg
     2521 aatataactc tgactttgta taatccggtg taatcataaa aaccgagacc ttaccagaaa
     2581 gtacaatgta aaaccattca ttatacctgc catgtcgtgc aacaatctca ttgtcattaa
     2641 actgatggat gttgatatta tgaactatct cattgagaca atcaaaagga acatcctcaa
     2701 aaaagttgag tgatcgtact atctcacatg gtgtcattga ttgtaaaaga gggaattttt
     2761 gcactgtata aataagctca tggatgggaa atatcttcat atagtatcct tcctcaatca
     2821 ataatgtgat accttaataa tagatagaga tatcattaat tgcaagtata aatgtataca
     2881 atagtactgt actactgtat atctgaaccg ggttcggggc gggcacttac ctcgtttgat
     2941 ggctttgact catggttgaa tagtgtatcg ggtttgtagc tatcatatgc agtaactgca
     3001 aaataataga taacgttatt ttttaataca ggatactgta aaaatccttt tgcatcatta
     3061 ttcctgtttt cctcaatgat ttcattggtt aatgttacac tgatataacc attgttatcc
     3121 gatagttcat tggtaattcg tttgttgcca atagtagtga taaccccatc atactttcca
     3181 cttttaacac catagtaaat tttatagccc aggatgtcgt actcaacatt cttcttccat
     3241 tttaacgtta tcaccctatc gctcacagaa tcaaccgcta ccatcacagg aggctgaggt
     3301 gctttatcaa gggcatactg taattctata gcatagatgg ttggtgcatt tgtaccatct
     3361 ggcgaagata tcagatgtgc cctccactga taatattttc ctctacagta actatcaccc
     3421 acctttttta gatatatatt tttctggtta ttgtttatcc tgtaccattg tggcagcata
     3481 tcgtcgcttt taaacatttc atcactgatc ctgaattcca tccatacata ggttgaaggg
     3541 ggcaactgct cctgccagtg aaacaaaagc accatcgtcc caaaatccgg aaattggtat
     3601 accgggcttg taataattcc ttcacgattt atggtatccc tattattata gcttaccggt
     3661 ttgtagtgcg acatggctat tgctgtttct ttttgtaaat tttcaatgta cctctgtgaa
     3721 attcttactt tgtcaataca cccataaaag ttcttcccca cagtggcaat gggcagatca
     3781 tgacagttaa attttggcac aagtacatca acaaatggct caccactttc agtacaatag
     3841 acagtttcta caacatcgcc attaatccat gtttcaagct tcccggagat tctgtcaaag
     3901 cttatagaaa tatgctgcca tgcattcaat ggtaatgatg gtccccggtg caaaaaaaca
     3961 tcaatcgggg tattgtcctt tttatagaac attttataaa atcgtacagc aagcttattt
     4021 tttacacaaa gcatctcaaa accctgttta ccctgtgaaa aaccagtacg cgataaaaca
     4081 atagcagccg agctatactg taacaatttt atggaagctt caatggtaaa tgaacctaaa
     4141 tcatcgcaat tggtcaacca gttgtttttt gccgtttgta tatcaacacg gtggtcacgg
     4201 tgaaaaaatt gtgcacaccc ttcaccttca attgcatcct gtaaatactt atactgagca
     4261 tagcgtatag cgtaatgacc ggaatcatct ttggccagat aaggaaaatc gctgtcaaat
     4321 gaaaggagca aatcggtaat cagtggatta accgattctt tataattgat tgttgctact
     4381 gtatagccag cggtattatt ttttaatcgc acctttttag cattaaaaag atcaagccca
     4441 tcaggcgtaa aaattattgt atacgctatt tcataagata ggccggtaac aaggcaacaa
     4501 cctataacaa gagctattat ccctatatag tgttgtatag ttttttttat catgagttag
     4561 tgttctatct gttgtatttt atccaaccgt tgctggtgac ggccaccttc aaaggttgca
     4621 tgcaaaaatg cattgatgat agctatggct aaatcaatac caacaacacg tgctcccagc
     4681 acaagcatat tggcatcatt gtgtttacgt gccatggttg ccatatactc attagtacaa
     4741 agagccgcac gcacccctct atgcttgttg gcaacaatac tggcgccaat tcccgtaccg
     4801 cagatggcaa ttccgcaatc aacctcttct cgttggagca tttcagcaac cagcttgata
     4861 taatcggggt aatcgcacga ttcatacgaa tcggtccctg cattgataaa aatgtgttct
     4921 ggtatagttt ttataatact atattttaaa tcaacacccg cgtgatcgtt gcctatacct
     4981 attttcatgg tttatcgctc caaaaataga aaagtaatga tgcaatatca aatgacaaat
     5041 ccttacaatg aaaaaattct ttgcctgaac tacgtttagc acaggagcta ccacttcagt
     5101 ataactatcg gcttcgactc aacattatac tactaataca atatctacta tacaaactat
     5161 catatcaaat aaattaacta actaactaac taactaacta actaaaattt tgcgcttccc
     5221 ggagtccgtg gaaacgggat gacatccctg atattctgca tccctgttat aaactgtatt
     5281 gctctttcaa aacccaatcc aaatccagca tggggagccg aaccatatcg tcgcgtatcc
     5341 agataccacc agtaatcatc aggattaagc cccatagcct gcatgcgctg tagcaataca
     5401 tcatatcggt cttcgcgctc actgccacca ataatctcgc caaccccggg tactaaaaca
     5461 tccattgcct ttacagtttt attatcgtca ttccgtttca tataaaaagc ttttatctcc
     5521 ttgggataat ctataacaat gattggcttt ttataatatt tttcggttag ccaccgttca
     5581 tgttcggtct ggaggtctat cccccatttt actgcatatt caaatggttc tggcgattgc
     5641 tttaattgcg caatagcatc ggtataggta actatttcaa atgaactggt gataatcgat
     5701 tctaaattgt gtagtatccc gggttttatc cgctcatcaa aaaaaaccat gtcctcatga
     5761 catctatcaa taacccatgt aaaaatatac ttaagaaatt cttctgccag agtgcaatca
     5821 tcttccaggg ttgcaaacgc catctcgggt tccaccatcc agaattcgga tagatgccgt
     5881 gtagtgttgg agttttcagc tctaaatgtt gggccaaagg tatagatatc acccatcgct
     5941 aatgctaaaa gctcaccttc aagttgacca ctgacggtaa gcgctgcttt tgctccaaaa
     6001 aaatcctggc tataatctat gaaaccatcc tgtactggta catgcagcag atcaagggtg
     6061 gttacctgaa acatctcacc agctccctca caatcacttg ttgtaataat gggggtatga
     6121 acatataaaa atcctttttg ctgaaaaaat gtgtggatcg catatgatac ttgattgcgt
     6181 atgcgcagga cagctcccat cgtattggtt cttggacgca gatgggcaat ctcccgtaaa
     6241 aattcaaaag aatgtcgctt cttctgcagg ggatagctct cgggatcagc atcaccgtat
     6301 acgatgagct tatccggctt aagttcccac ttctgtcctg ctgcaggaga tgctaccagt
     6361 ttgcctgtta tataaacgct ggcaccggta tgcagctgcc gtgcagtttc atagcaacta
     6421 tcgccaaaac caaccaccac ctgcatattg gttaaacacg agccatcatt tatttcaata
     6481 aagatatttt ctttggattc ccttttggta cgcacccagc ctgccaccgt gcaggactcc
     6541 aaacttttat cactttgtaa acaatcaaca atacgtatgc gcttcatcac ctgctccgct
     6601 gtcatgtttt aattttttga ttccatgcta ataacagcac tttcactcat tttgaacatt
     6661 caatatagca aacatcatca attcaaaaat aaaaaataat agaagtacta tttgttttat
     6721 attacatcat tgcccttcct tacgcttctt taataacagt gcttccttta atcccttttc
     6781 acgtatcact ttaatcttta aacctaactc ggtcatttta aaatatcgtt ttccatattt
     6841 tgaaagcaac tttttgtcaa tctcaaaacc caatcctgga cttacaaatg gttgcagcat
     6901 tgcattttta tctggcagta taggttctat tatgagtttg cggatatccg ggcatccacg
     6961 ttggccagaa tgtataccgt aaaggtatcg aaacatggta taactctata caacgtatct
     7021 tcataataac cctcctcctt tttaattttt tttgattcta tcatccaata atcatgcgtc
     7081 aatcataatt cataatacct gttgttaccc cgacgttttt ttacagcaac aacatctggc
     7141 atatgaaaaa aaataattta caggtgattt ttattttttt aaaaaaatat ggttaatcat
     7201 ttttaataga ttatttatta ttgacaatat tttataaaca gacactttat aagtactata
     7261 tatgtaccga tagttattga caatagatct attagcagag gaaaatcatg cgattattgt
     7321 atattacttt attattactg tttactgtca gtacgttgca tgcgcaggat acggtgtgtg
     7381 ttctggtata tcatacattt atgggaaaaa agattcacta tgacatctca ctggaagaat
     7441 tccacaaaca gttgttggaa ctgaaagatc atggttttac ctttgttacc tttcatgaaa
     7501 tcaaaaacgg actggtaaaa ggcaataaaa atattctggt aaccatcgat gatgggcata
     7561 gaacagctct tgacgcctat tattcagtgt taaaaccaat gaggattaaa ccgttattgg
     7621 gaatcaatac ttttattatt ggtaaaaagc cgtatgtttt gacatgggat gagttaaaac
     7681 agctttctcg tgaaggctgc tatatagcct cacatggtta cttccatctt tttataaatg
     7741 ataagcttta taaaaacaac tatcgttatt ttaaattaga agtatttgaa tcaaaaaaaa
     7801 tgctggagga aaaactatgc acacccgttg atacctttat atatcctttt ggtgttgttt
     7861 ctgacgttgg taaaaaagct ttgattgaag cgggatataa ctacgcattt actatcaaat
     7921 ggggaaatac ttcattgcca ataacaagca ataatctgga aatatcccga tacatgtata
     7981 atcaaaactg gaacgccata gccagagcca ttatttataa acacaaaaac ccatctaccc
     8041 ctcataaaga gagcgaaaag attcttgttt ctaccatgcc ccggttgaaa ggtactcatc
     8101 aatagctttg gctgcctttc gtcctgcacc cattgcctgt ataactgttg cagcaccggt
     8161 tacaatatcg ccgccagcaa aaacgcctat cttgcttgtc ttcatggttt cgggatcggc
     8221 tatgatattt cccctctttg ttatattcat gccgggtgtt gttttgggga ttaacggatt
     8281 tgggccattc ccaatggcaa taatggctac atcaatagca atggtaaact ctgaaccttt
     8341 cacagggatt gggcttcgtc tgccacttgc atcaggttca cccaattcca tcttcagaca
     8401 ctcaactgct ttcacacgtc ctttttcatc accaatgaac tgtacaggat tggttaacaa
     8461 tgtaaaaata acgccttctt cctcggcacg ttccacctct tctactcgtg ccggcatctc
     8521 agtgcgggaa cgccggtaaa caatatacac ctccttgctt cccaaacgca gtgcagtccg
     8581 tgccgaatcc atggcaacat ttcctccgcc aaaaactgca accttctgcc ctttgacaat
     8641 gggagtatca tactccggga atttatacgc cttcatcaaa tttgagcgtg tcaaatactc
     8701 attggcactg tacaccccct gtaaattttc accgggtata tctaaaaata atggcaaccc
     8761 ggctcctgta gctacaaaca cagcatcaaa ccccgttgac agtagatcat ttaccgtttc
     8821 agccgtgcca attaccctgc ttagtccaat agttacacct gcagctttca gtgcatcaat
     8881 ctcatactgc acaatatctt tgggaagtct gaactcagga atcccataca ccagaacgcc
     8941 accagcctta tgtaatgcct caaaaatggt aacatcgtac cctatacgtg ctaaatcacc
     9001 ggctgcagta agccctgctg gcccggagcc aatgattgca acctttttat tggtatggct
     9061 atcgctcttc tgagccacaa aatgttcctt ctctcgttca taatcagcaa catagcgctc
     9121 cagattgcca atagcaacag catcgccttt ttttgccagc acacacaatt tttcacattg
     9181 atcttcctgg gggcataccc tcccgcatac tgccggcaat gaatttgttt gttttatggt
     9241 aaaaaatgcc tcgagaggcc ttccctcttt caacgccttt ataaatcctg gaatattgac
     9301 attaacagga catccatcaa cacaggcagg acgcttacac tgcaaacacc gtgctgcctc
     9361 taccatagct gttttttcat cataccctaa cgggacctca ttgaaattgc ggatacgatt
     9421 ttttgcatcc tgctgaggca ttttctgccg tggaatttta tctgccatag tactgctcca
     9481 gtttgcattt atgtttttca aagctttctt tttcctgctc tttataggta ttaagccttt
     9541 tcataagttc atcaaaatca accagatgag catcaaactc aggaccatca acgcaggtaa
     9601 attgcttttt gccatcaacc gtaaccctgc atccgccgca cattccggtg ccatccacca
     9661 ttatggaatt taagctgacc agtgttttca ccccatactg tttggtcata tcactgaccg
     9721 ccttcatcat tggaaccggc ccaacagcaa caacaatatc aactttcccc ccatcaagta
     9781 tcgttttcaa ttcatcggtt acaaatcctt taacaccatg cgacccatca tccgtagtta
     9841 ctatcatctc atcacttttt gctttgagtt cgttttctaa tattaataaa tttttgttgc
     9901 gcgctccaat aatacttatg agatagttac ctgcatcttt cattgccact gcaattggat
     9961 acacagcagc aattccaatg ccaccaccaa cgcaaacaca ggtaccatag cattgtatat
    10021 cggtaggatg ccccaatggt cctgcaacat taaaaagttc ctcccccttc tttttaagcg
    10081 atagtttatg ggtagaggtc cccacaatct gatagtaaag tgtgatagtt cctgtagcac
    10141 tatcagcatc agcgatagta agcgggatac gttcacccac ttcatcaaca ataactatta
    10201 caaattgccc tgcttttctg tgttttgcaa tatagggtgc ttccacaata gcttttccta
    10261 cattgtcaga tattttttca ttggttacta tcgtatacat aattcacttc cataataaag
    10321 ataatcaaac aattgatatg cataatgaaa tattgaacat gtcatgaact tactagcaat
    10381 catctgacct ttgacagaca tagtatatag tatatcacat tctattgatt aaaaaataac
    10441 tatcgccaat acaatactgt cacaaatact ccctgtcaaa aagcatatac caacactgat
    10501 gactatacac catttgttgc aggcaaacag tacactataa tagctacaca tggtcaagat
    10561 atttttatat aatcaggctg tcgaaataat gtacataaca aggatagtat gccattagta
    10621 taccgtaatc aagcgacaaa aacagggaga acgtgatgag acgattcagg gaaccgcgtc
    10681 atagaccact cgacgatagg gaagtttttg gttcgtttca caaacgagct tatagagatg
    10741 tttgtacaaa cggataagct tgccattgag aaggggctgg cgaattgtaa agtattgtca
    10801 attgattcgg tcaagatacg ggcgaatgcg tcaagcaaga aggacacgag cattgatggt
    10861 ttgaa
//
LOCUS       DAOSOX010000078        10810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6079_length_10810_cov_9.089912, whole genome shotgun sequence.
ACCESSION   DAOSOX010000078 DAOSOX010000000
VERSION     DAOSOX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6079_length_10810_cov_9.089912"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLX22_06975"
     CDS             <1..327
                     /locus_tag="PLX22_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19679.1"
                     /translation="VVYSNEDVERSVYMVNHAIDTILDMCISQEELTKTIEENINTVL
                     QGSTEITNATSEEKAAISEITATINQLLTITNTVYENTKILSETLSKITMQIDSLQSI
                     AEIQSK"
     gene            440..1771
                     /locus_tag="PLX22_06980"
     CDS             440..1771
                     /locus_tag="PLX22_06980"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOT19680.1"
                     /translation="MCDTIVATQKATADGSVILAKNSDREANEAQLLKYIARRSWAND
                     TTVQCTYATIPQVKETNEIFISKPFWMWGCEMGANAHGLAIGNEAVFTKEPYEKTGLL
                     GMDLIRLSLERCNNAYDALMLTTELIEQYGQGGNAGMAHKLFYHNSFIFADSSCAYVL
                     ETANRHWVAIKVDGIRSISNGLTIEEEFTFASKGIEDYALKKGYLKKGDTFNFKKCFS
                     DTLYTYFSRCSDRQSRTTSCASKNSGTITPQSMFALLRDHGNDDMQLPPDKTSMGSVC
                     MHASLLPTRPSQSVAAFVAHLRDNIPAFWATGSSGTCTSVFMPFYLIGKDIDYFPAKE
                     TSTYSPESYWWMHEKIHRQSIFKWDYFQKTIAPSIISIENEFITKDNELFKKKNTTTQ
                     KYFDFSRLCVEKVLNINERWEKELKSAPYSNTKLSFTLFWNSQTKKAMIPV"
     gene            complement(1774..2439)
                     /locus_tag="PLX22_06985"
     CDS             complement(1774..2439)
                     /locus_tag="PLX22_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19681.1"
                     /translation="MKICDGIFGYIWRGVFENNCNTFYLGEPLNMLIDPGLAKYVDMH
                     FTDMQKDGIDTKAIDYVIATHSHPDHFEGCAMFMGKGPQVVMHKDEIAFLHEVGPQFF
                     AMFGMQFPQFTFDIVLEEGEWKIGNEVLQVYKTPGHSPGSMCLYWASKKVLVCGDLIF
                     KESVGRTDFPGGDGTLLKESIQRMKELDIEYLLPGHMDIVVGKNNIKKNFELIEKYIF
                     PML"
     gene            complement(2443..>3291)
                     /locus_tag="PLX22_06990"
     CDS             complement(2443..>3291)
                     /locus_tag="PLX22_06990"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOT19682.1"
                     /translation="QGQIGKIYALLDGEDSEVADAIDFQYRPRFQGDILPSSIVSIVV
                     SLAEKIDNIFGSFSVGNIPKGSADPYALRRQSAAIVDMLVGNAIHLDLANVCKIIATQ
                     YNNGAQLVDAILDFINGRAKTFLYEKGFAFDEVNACLAVGCYDYYELYLRAQSIHEFR
                     RDKRFGDLLIAFKRMFNIVNAFLKKNPGYTFSFNPDALVIEEEKNLYTFFKEKEDSIQ
                     EYIAHNKYKDLFLLLIEGKEAIDTYFDKVMVMDSNPVVRDNRLGMLQSILTLFTAFID
                     FSQIQE"
     assembly_gap    3292..3391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3392..4670)
                     /gene="glyS"
                     /locus_tag="PLX22_06995"
     CDS             complement(<3392..4670)
                     /gene="glyS"
                     /locus_tag="PLX22_06995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOT19683.1"
                     /translation="MERINFVCEIGTEEIPAGYIPPAIQQIAALMSEGLKTHRIASGS
                     CMVYATPRRIAVCVSDMADKQSEDYEELKGPSVKAAYDAEGNPTKAFMGFLEGNKIKL
                     EDTFIQNTSKGDYIYARRMVEAKPVTAIIPEMIEQIIMQVNFPKRMRWNVKRISFPRP
                     IDYICVLFNDTVVMHSIDGIPFDNKVRGHYIQNNCMIPLHYCREYGEVLEKHGVIVDH
                     NKRKESIKQQLYSAAKTLQGKLVEDEELLDTVTFLTESPYVVICSFDESFLTIPDIVL
                     ITEMKEHQKYFAIRDDNGKLLPKFLVVSNNPPTEHIVKGNQRVIRARFTDARFFFEED
                     RKIPLADRVDMLKSILFHKELGSIYDKVVRVQKIAEVISQQLSFDDATMKKIDRAVLL
                     AKTDLTTSLVFEFTSLQGQIGKIYALLDGEDSEV"
     gene            complement(4682..5692)
                     /locus_tag="PLX22_07000"
     CDS             complement(4682..5692)
                     /locus_tag="PLX22_07000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19684.1"
                     /translation="MRSWHLHNYIVGDISITLLSSEIIIIHRRKNNAFIKSISWRSFR
                     RAGIAFAAVCLCLVVLFILAGINDIFFSEASWYDEIKKKTIANKEFEEPRERSILKIV
                     HHTIKQGETLSEIAGMYGVSMDTICGCNKLESYDLVHVGQVLTIPNKDGLIVSVQSGQ
                     TLPSLARKYNVGIQKIIAENVIANPDFINVGQHIFIPDAKPLDIFKGFLWPVNNQYIT
                     SGYGWRKDPVYGDKQFHQGIDVRANYEWIHASKYGRISYAGWLGGYGKVIVVTHPGGD
                     RTLYGHLSRIIVKEGQYVKQGQIIAKSGNTGNSTGPHLHFEIIRKGQHINPYVELKKK
                     RK"
     gene            complement(5724..6149)
                     /locus_tag="PLX22_07005"
     CDS             complement(5724..6149)
                     /locus_tag="PLX22_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT19685.1"
                     /translation="MAGKSDSLNSTIGEGSVFEGKFYISGSLRIDGKFEGEIKTDEEL
                     VVGETGKVKTNIHAKNVVVAGTVIGNIVADEEVKLLETAKVLGDIETPILTVQRGVVS
                     QGVVTITGGQKKDVKKLIEESYAGTPQAAGIFSKKGSEK"
     gene            6346..7002
                     /locus_tag="PLX22_07010"
     CDS             6346..7002
                     /locus_tag="PLX22_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19686.1"
                     /translation="MNILQTPIVVNEIFYSLQGETTRTGFPSLFIRLAGCNLNCRWCD
                     TAYARTGYTMTIASIVKKIQQYPLANHITITGGEPLIQQGTIALLNILCTHNYPLQLE
                     TNGSISLEKVPQPVHRIVDVKTPSSGEEKSFNFTNLQLLQPHDEIKFVIANDNDYNYA
                     KEFMQKYCANTCCVINFSPVVLTMKPQTLAEYILRDRLPVRLNVQLHTMLWQQEPKHF
                     "
     gene            7012..7884
                     /locus_tag="PLX22_07015"
     CDS             7012..7884
                     /locus_tag="PLX22_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19687.1"
                     /translation="MDYVGHIIRPPSEAYSMIIQVTVGCSHNMCTFCGTYKDQKFYVK
                     DMQQVKRDIDEASRYRFDRVFLTDGDVLILPTETLLDLFAYIKKKNPHIERIGVYANT
                     KAILKKSVAELKQLKDAGLGIVYHGIESGNEEVLRRIRKGALPHKQLEAAQKIKEAGI
                     ALSQTVLLGIGGIDLSIPHAMDTGKHLGAMSPDYASALTVMLLPNTPLYRDYVQGKFV
                     LPDKFGLLRELKLMIENMDVTSTCYFTSNHASNYLPIKATLPDDKIQVISLLDHIINT
                     KDESILKPEYLRAL"
     gene            7899..8060
                     /locus_tag="PLX22_07020"
     CDS             7899..8060
                     /locus_tag="PLX22_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19688.1"
                     /translation="MDKSMEEAIRFISFELQESPGTDIAKLIEKASQQFDLTPMQTEF
                     LVNKFILNK"
     gene            complement(8296..9378)
                     /locus_tag="PLX22_07025"
     CDS             complement(8296..9378)
                     /locus_tag="PLX22_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT19689.1"
                     /translation="MIRGLVYLVIISLAIIGSCAKQKVDDYASIAFSIGDVKKNGQLA
                     VIGEAINQRDIIETGALSSCDIKIGDSIIRIKEKSKAIMAQFLRKDGIENTTLGLDIG
                     KMICKPKKLLKNESFLVKTPTAVAGVRGTNFSVEADAKKTTRIKVFEGKVAVVKRVEA
                     VEEHVDKIIEVAPVVEEKEKVIITADDVKKAEKKVEVIIAKEGESPKAIEKVIAVAKE
                     EIVVSRDKVEKFKPEDFNDEKQGIIKVEEKPKEVVKEVAKVVKKDKQIPKPEGQLLVT
                     RYEIYFVKNGRVEWEGKVVSPPTKADDKIYIASGDYIFCAKLDGTVLWRKKLANDGKI
                     ELEGEKLAVYAGGQKKILDKLTGEEE"
     gene            complement(9644..10393)
                     /locus_tag="PLX22_07030"
     CDS             complement(9644..10393)
                     /locus_tag="PLX22_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT19690.1"
                     /translation="MLRVEHLFAGYDSKIVLKDISLQLHRGTVAALLGPNGSGKSTLL
                     KAIDGIIKPDNGAIILDTMNINAMTKNNIAQSIAYLPQSGNSFPSCNVFESILLGRKP
                     YISFEPTKHDMEIVEKTINNFNIDSLAFRTINQLSGGEIQKVLIARAIVQEPEVLLLD
                     EPINHLDIKNQIEILQIVRYYTINYNLITLIVFHDINRALQFADKFIVLKEGYVYGDG
                     DSKIVNPEIMKGVYGIDLDCIEYKGRKLLFV"
     gene            complement(10393..>10810)
                     /locus_tag="PLX22_07035"
     CDS             complement(10393..>10810)
                     /locus_tag="PLX22_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOT19691.1"
                     /translation="KLIMTIGFIACFVYFYIHRWNYNALLWGDDVAKSLGVNSTSLRL
                     LGMLLCALIVSLCTAFLGIIGFVGLIAPHIVWMIIGNDYRFLIPYSALFGALFLLISD
                     SIAKMLLSPVILPVGIITSFLGAPMFFYLLVRRSTL"
BASE COUNT         3353 a         2329 c         1685 g         3343 t
ORIGIN      
        1 gttgtctact ccaatgaaga tgttgagcgt tctgtatata tggtaaatca tgctatcgat
       61 accatccttg atatgtgcat ttcacaggaa gagcttacta aaacaattga agaaaatatc
      121 aatacagttt tgcagggttc cactgaaatc actaacgcta ccagtgaaga aaaagcagcc
      181 atcagcgaaa ttacagcaac aataaaccag cttctaacca taacgaatac tgtatatgaa
      241 aatacaaaaa tactatcaga aactctatca aagataacca tgcaaattga ttcattgcaa
      301 tccattgctg aaattcaatc taaataacta tttatgaaaa ctatttttat ttagtacttc
      361 atatagcaat atcaaaaata ttttgacttt tttattttat tgctatacag tgttacttat
      421 ataatgaaag gagattgtta tgtgtgacac cattgttgca acgcaaaaag caactgctga
      481 tggtagcgta atccttgcca agaacagcga ccgcgaagcc aatgaagctc agttgttaaa
      541 atatattgcc aggcgttcat gggccaatga tacaactgtt caatgtacct atgccaccat
      601 acctcaggtt aaggaaacca atgaaatttt tatttcaaag ccgttctgga tgtggggttg
      661 cgagatggga gctaatgctc atggattggc cattgggaat gaagcggtat ttacaaaaga
      721 accatatgaa aaaacaggat tgctgggtat ggatcttata aggctttccc ttgagcgttg
      781 caacaatgcc tatgatgcac tgatgctaac cactgaactc attgagcaat atggacaggg
      841 tggcaatgca ggcatggctc acaaattgtt ttaccataat tcttttattt ttgccgatag
      901 ttcctgtgca tacgttcttg agactgcaaa taggcactgg gtagcaatca aggttgacgg
      961 tatacgcagc atttcaaatg gattaactat tgaggaagaa tttacctttg catcaaaagg
     1021 catagaagac tatgcattga aaaaggggta tttaaaaaaa ggtgatactt ttaattttaa
     1081 gaaatgtttc agtgacacat tgtataccta tttttcacgt tgctctgaca gacaatcaag
     1141 gactacatca tgtgccagta aaaatagcgg tacaattaca cctcaaagta tgtttgcatt
     1201 gctcagggat catgggaacg atgatatgca acttcctcca gataaaactt caatgggttc
     1261 tgtatgtatg catgcttctc ttctaccaac gcgcccaagt cagtcggttg ctgcatttgt
     1321 tgcacatctc agggacaata tccccgcttt ttgggcaacc ggttcatcag gaacatgcac
     1381 cagtgtcttt atgccatttt atctgattgg caaagatatc gattatttcc ccgcaaaaga
     1441 aacaagtacc tattcacccg aaagttactg gtggatgcat gaaaaaatac accgccagtc
     1501 aatttttaaa tgggattact ttcaaaaaac tatcgcacca tcaataatat ctattgaaaa
     1561 tgaattcatc acaaaagaca atgagttgtt taaaaagaag aatactacca ctcagaaata
     1621 ttttgatttt tccagactat gcgttgaaaa agttctaaat atcaatgaac gatgggaaaa
     1681 agaattaaaa agcgctccct attcaaatac aaaattatcg tttacattat tctggaattc
     1741 gcaaacaaaa aaggcaatga tcccagtatg atgctataac attgggaata tatatttttc
     1801 aatcaattca aaattctttt taatattgtt tttccctact acaatatcca tgtgccccgg
     1861 caacaggtat tcgatatcaa gctctttcat ccgctgtata ctttctttga gcaatgtgcc
     1921 atccccaccg gggaaatcag ttcttcctac actttcttta aatatcaggt cgccacaaac
     1981 aagcaccttc ttagaagccc agtataaaca cattgaacct ggcgaatgtc ccggtgtctt
     2041 ataaacctgc aatacctcat tgcctatctt ccactccccc tcttccagca caatatcaaa
     2101 tgtaaattgt ggaaactgca tcccgaacat tgcaaagaat tgaggaccaa cctcatgtaa
     2161 aaacgctatt tcatccttat gcataacaac ctgggggccc tttcccatga acatcgcaca
     2221 tccttcaaag tgatccggat gtgaatgcgt agctataaca taatcaattg ctttcgtatc
     2281 aataccgtcc ttctgcatat cggtaaaatg catatcaaca tattttgcca gcccggggtc
     2341 aatcagcata ttaagaggtt cgcctagata aaatgtattg cagttatttt caaaaacacc
     2401 acgccatata tatccaaata taccatcaca tattttcata ttctactcct gaatctgtga
     2461 aaaatcaatg aaagctgtaa acaatgtaag tattgactgt aacattccca gcctattatc
     2521 gcggacaaca ggattactgt ccataaccat aaccttatca aaataggtat caatagcctc
     2581 tttgccttct atgaggagca aaaataggtc tttgtactta ttgtgtgcta tgtattcctg
     2641 aatgctatcc tctttttctt taaaaaacgt atataaattc ttttcttctt caatgacaag
     2701 cgcatcagga ttaaatgaaa atgtgtatcc agggttcttt tttaaaaatg cattgacaat
     2761 attaaacata cgtttaaatg ctatgagaag atccccaaac cgtttatccc ttctgaattc
     2821 atgtatgctc tgtgctctca gatatagctc atagtaatca taacatccta ctgccaaaca
     2881 tgcattgacc tcatcaaagg caaaaccctt ttcatataaa aatgtttttg ccctcccatt
     2941 aataaaatca agaatcgcat caaccaattg ggcaccatta ttatactgtg tggctattat
     3001 tttacaaaca tttgcaagat ccagatggat agcattccca acaagcatat caacaattgc
     3061 tgctgactga cgtcgcagtg cataaggatc tgcagagcct tttggtatat taccaacgga
     3121 aaatgaacca aaaatattat caattttttc agctaacgat acaacaatac tcacaatact
     3181 tgaaggtagt atatcaccct gaaatcgtgg ccggtattgg aaatcaatag catcggcaac
     3241 ctcactatcc tcgccatcaa gaagcgcata aattttacca atttgcccct gnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaacctcac tatcctcgcc atcaagaagc
     3421 gcataaattt taccaatttg cccctgtaag gacgtaaact caaagacaag ggacgtcgta
     3481 aggtcggtct ttgccaatag cacagcacga tctatctttt tcatagtagc atcatcgaag
     3541 gatagttgct gacttataac ctcagctatc ttctgcaccc ttactacttt atcatagata
     3601 gagcccagct ctttatggaa aaggatagac tttaacatat caacacgatc agctaatggg
     3661 atctttctat cctcttcaaa gaaaaatcgt gcatccgtga agcgtgccct gataacacgc
     3721 tgatttcctt tcacaatatg ctctgttggt ggattatttg atacaactaa aaattttggc
     3781 aatagtttgc cgttatcatc acggatggca aaatacttct ggtgctcctt catctcagta
     3841 ataaggacta tatcaggaat agttaagaac gactcatcaa aactacagat aactacatag
     3901 gggctttctg ttaaaaaggt aacagtgtcc aggagttctt catcttccac cagtttgccc
     3961 tgcaaggttt tagcagcact atacaattgt tgtttaatac tctccttccg cttattgtga
     4021 tcaactatca ccccatgttt ttctaaaact tcaccatact cccggcaata atgcagggga
     4081 atcatacaat tgttctggat ataatgccct ctgactttat tatcaaacgg tattccatca
     4141 attgaatgca ttacaaccgt atcattgaag aggacacaga tataatcaat aggtcgggga
     4201 aaactaatcc gtttaacatt ccagcgcata cgtttgggaa aatttacctg cataatgatt
     4261 tgctcaatca tctccggaat aattgctgtt accggctttg cttctaccat gcgtctggca
     4321 tagatataat cacccttact tgtattctga ataaatgtat cttccaattt tattttgttc
     4381 ccttctaaaa accccatgaa tgcctttgtg gggtttccct ctgcgtcata ggcagcctta
     4441 accgatggac cttttaattc ttcataatct tccgattgct tatcagccat atcgctgaca
     4501 catactgcaa tgcgccgtgg cgttgcataa accatacacg agccagatgc aatcctgtgc
     4561 gttttcaaac cttcactcat aagtgcggct atttgctgta tagcaggagg aatatatcct
     4621 gcaggtatct cttcagtacc aatctcgcaa acaaagttaa tcctctccat atccttttat
     4681 cctactttcg tttctttttt aattccacat aaggatttat atgctgacct ttcctgataa
     4741 tttcaaagtg tagatgcggg ccggtagagt tgccagtatt accactctta gcaataatct
     4801 gcccctgttt tacatattga ccttctttca caattattct ggacagatgc ccatacaatg
     4861 ttctatcgcc cccgggatga gttacgacaa tcaccttacc atatccccct aaccagcctg
     4921 catagcttat ccgcccatat ttgctggcat gtatccattc atagtttgct ctaacatcaa
     4981 taccctgatg gaactgctta tcaccatata cgggatcttt acgccatcca tagccactgg
     5041 taatatattg attgttaaca ggccatagaa accccttgaa aatatcaagc ggctttgcat
     5101 cagggataaa gatatgctgc ccaacattga taaaatcagg attagctatt acattctcag
     5161 caattatttt ttgtatccct acattgtact ttctggcaag agaaggaagc gtctgaccgc
     5221 tttgtacact aactatcaat ccatctttat tggggatggt taaaacctgt ccaacatgca
     5281 caaggtcata tgattccagc ttattacatc cacaaatcgt atccatcgaa acaccataca
     5341 ttccagcaat ctcgcttaat gtttcaccct gctttatagt atggtgtaca atctttaata
     5401 tggaacgttc acggggttcc tcaaattctt tattggcaat ggtctttttc ttaatttcat
     5461 cataccatga agcttctgaa aaaaatatat cgttaatacc agccaaaata aaaagcacaa
     5521 caaggcacaa acaaacagca gcaaatgcta tccccgctct cctgaaactg cgccatgaaa
     5581 tgcttttaat aaaggcattg tttttccgtc tgtggataat aattatctca gatgataaaa
     5641 gtgttatgct tatatcacct actatataat tatgtaaatg ccacgatctc atagattcat
     5701 tatcggcaac aaagaatcag tcgtcatttt tcagaaccct tcttgctgaa aattcccgca
     5761 gcctgcggcg ttccagcgta cgattcttca ataagcttct tcacatcttt cttttgtcca
     5821 cctgttatgg taaccacacc ctgagaaacc acaccacgct gtacagtcag tattggtgtt
     5881 tcaatatcac ccagcacctt ggctgtttcc agtaatttaa cctcttcatc agcaacaata
     5941 ttcccaatga cagtccctgc aacaacaaca tttttggcat gtatatttgt cttaaccttt
     6001 ccggtttcac ctactacaag ctcctcatcg gttttgatct caccttcaaa tttcccatca
     6061 atacgaagcg aaccactgat ataaaattta ccttcaaaaa ccgaaccttc gccaatagta
     6121 ctattcaagc tatcactttt accagccata cgtttatcac ctcttgctta ttaatttcct
     6181 tgttgcatta tttttttgaa tgtacatgta cttttgaatt attgcaattt tatttcaact
     6241 attttttatt gcaaatcaat tattcctgta ctatttttac taaaagcaat actattatgt
     6301 gcgatagtta ctgataatta actgcaacct gatgacatag aatatatgaa catattacaa
     6361 acacccatcg ttgtcaatga gatattctac tccctgcagg gtgaaactac ccgtactgga
     6421 tttccgtctc tttttatacg gctggcaggc tgtaacctca attgcaggtg gtgcgacact
     6481 gcctatgcaa ggaccggtta tacaatgact atcgccagta ttgtaaaaaa aatacaacag
     6541 tacccattag ccaaccatat taccatcact ggtggcgaac ctctgataca gcagggcacg
     6601 atagctttgc tgaatattct ttgcacacat aactatcctc ttcagcttga aaccaatggc
     6661 agtatttcac tggaaaaagt accgcaaccc gtacacagga tagtagatgt taaaacacct
     6721 tcaagcggcg aggaaaagtc atttaacttt acaaacctgc aattattgca gccacacgat
     6781 gaaataaaat ttgtgatagc caatgacaat gactataatt atgctaagga gtttatgcaa
     6841 aaatattgtg ccaatacatg ctgtgttatt aacttttcac cggtagtgtt aaccatgaag
     6901 ccgcagacac ttgcagaata tattctgcgt gacagattac cggtacggct taacgttcag
     6961 ttgcatacaa tgttatggca gcaagaaccc aaacattttt aggagcaagc gatggactac
     7021 gtaggacata tcatacgccc cccttcagaa gcgtatagta tgattattca ggtaaccgtt
     7081 gggtgctcgc acaacatgtg caccttttgc ggtacctata aggatcaaaa attttatgtc
     7141 aaagatatgc agcaggtgaa gcgtgatatt gatgaggctt cgcggtaccg ctttgatcgt
     7201 gtttttttaa ccgatggtga tgtgcttata ctgccaaccg aaacattgct ggatttattt
     7261 gcctacatta aaaaaaagaa tccgcatatt gagcgcattg gtgtttatgc caataccaaa
     7321 gccatactaa aaaaatcagt ggcggaatta aaacaactaa aagatgccgg gcttggcatt
     7381 gtataccatg gcattgaaag cggcaatgaa gaagtactac gcaggatacg caaaggggcg
     7441 cttccgcata aacagttaga ggcagcacaa aagattaaag aagcaggtat tgcgctatca
     7501 caaacagtgc tgctggggat tggtggtatt gatttaagca tcccccatgc catggacacc
     7561 ggcaaacatt taggtgccat gtcacccgac tatgcatcgg cattaaccgt tatgctttta
     7621 cccaatacac ccttataccg tgactatgta cagggcaaat ttgtattgcc cgataaattt
     7681 ggcttacttc gcgagctcaa gctcatgata gaaaatatgg atgtaaccag tacatgctat
     7741 tttacatcaa atcacgcatc taactattta cccattaaag ctacattgcc tgacgataaa
     7801 atacaggtta tttcgttgct tgaccatatt atcaatacaa aagacgaatc aatattaaaa
     7861 ccagaatatc tcagggcatt gtaaggaggg gatggatcat ggataagtca atggaagagg
     7921 ctatacgttt tattagcttt gaacttcagg agagcccggg caccgatatt gcaaagctta
     7981 ttgagaaagc cagtcagcag tttgacctta caccaatgca aaccgaattt ctggttaaca
     8041 aatttatatt aaataaatag taacgctctg caccgataga atgtacagta tgaagtatat
     8101 acaccgtaca ttgtcattca ggtctttacc cggaatccaa atatctatcg ttatatcctg
     8161 aatcagcgtt aaggatgaca tccaccgcca attttatttc atatggatgg caacacccat
     8221 gccataaaaa actcctcatc ttcaggttta ctgaaaatga ggagtttatg gcaaagcttt
     8281 tatttcaaaa tcaatctact cttcttcacc tgttaattta tccagtattt tcttctgccc
     8341 tcctgcatat actgcaagct tctcaccttc caattcaatc ttaccatcgt ttgccagttt
     8401 ctttcgccat agcactgttc catctaactt agcacagaag atataatcac ctgatgcaat
     8461 ataaattttg tcatcagcct ttgttggagg gcttacaacc ttgccttccc attcaaccct
     8521 tccattcttt acaaagtaaa tttcatatcg ggttaccagc agttgtcctt ccggttttgg
     8581 aatctgttta tccttcttta caacctttgc aacttcttta actacctctt ttggtttttc
     8641 ttcaaccttt atgattccct gcttttcatc attaaaatct tcaggtttaa atttttccac
     8701 cttatctcta cttaccacaa tctcctcttt agctactgca attaccttct ctattgcttt
     8761 aggcgactca ccttcctttg caataatcac ttcaaccttc ttttctgcct tttttacatc
     8821 atccgctgta ataattactt tttctttttc ctcaacaacg ggagctactt caataatttt
     8881 atcaacatgt tcttcaactg cttctaccct ctttactaca gccaccttcc cttcaaaaac
     8941 cttgatacgt gttgtttttt tagcatcagc ttcaaccgaa aaatttgtgc cgcgtacacc
     9001 agcaactgcg gttggagttt tcactaaaaa actttcattt tttaaaagct ttttaggttt
     9061 gcatatcatc ttcccaatat ccagaccaag tgttgtgttt tcaatgccat cctttcgcaa
     9121 aaattgtgcc ataatagctt ttgatttttc ttttatcctg attatggaat caccaatttt
     9181 tatatcacat gacgaaagtg cacctgtttc aataatatcc ctttgattta tcgcttcgcc
     9241 gataactgct aactgcccat tcttcttcac atcacctata ctgaacgcta ttgatgcata
     9301 atcatcaacc ttctgctttg cgcaactgcc tataatggct aaacttataa ttaccaaata
     9361 tactaaaccc cgtatcattg tatatcctcc attaaagtaa agataaaata ttgcacattt
     9421 tatagatttg aacaaataca acaaactata accaaccttc ttaattacaa tcatttaagt
     9481 atacatgtat ttgtagaaaa aatgcaaatt ttttttataa tttataaaaa atcatttttt
     9541 atatatcact ttcccataat ctctgaaatt tttgtaaacg gataaggatt atacaacaaa
     9601 cttttctttt acaatttgag atagtaaata cctattatac tatttataca aataataact
     9661 tccttccctt gtattcaata caatctaaat caataccata aacacctttc attatttcag
     9721 gattaacaat tttggaatca ccatcaccat agacatatcc ctctttcaac actataaatt
     9781 tatctgcaaa ttgcagtgct ctgttaatgt catgaaacac tattaacgta attaaattgt
     9841 aattgattgt ataatacctt actatctgta aaatttcaat ctggtttttg atatccagat
     9901 ggttgatagg ttcatccagt aataaaacct caggctcctg cacaatggct cgtgcaatca
     9961 aaaccttttg tatttctcca ccactcaatt gatttatggt tctaaatgct aatgaatcta
    10021 tattaaaatt attaatggtt ttctcaacaa tttccatgtc atgctttgtt ggttcaaatg
    10081 atatatatgg ttttcgccca agaagtattg attcaaatac gttgcatgat gggaaactat
    10141 tgcctgattg aggtagatat gcgatagatt gtgcgatatt gttttttgtc attgcattaa
    10201 tattcattgt atccaggata atagcaccgt tatcaggttt tattattcca tctattgcct
    10261 ttaaaagcgt tgatttacct gaaccatttg gaccaagtaa tgctgccact gtacctctat
    10321 gcaattgcaa cgaaatatct ttcaaaacta tttttgaatc atatccagca aaaagatgtt
    10381 ctactcttaa cactataacg tactccttct taccagtaga taaaaaaaca ttggtgcacc
    10441 cagaaatgaa gttataatcc caaccggaag aattactggt gatagaagca tttttgcgat
    10501 actatcagat attaaaagga ataaagcgcc aaacaaagct gaatatggta taaggaaacg
    10561 ataatcatta ccaataatca tccaaacaat atgcggagca atgagcccaa caaatccaat
    10621 gatacctaaa aaagcagtac agagtgaaac tatcagtgcg cataataaca tccccagtaa
    10681 gcgtaaggat gtactattta cacccaaact ttttgcaaca tcatcacccc ataataatgc
    10741 attataattc catcgatgaa tgtaaaaata aacaaaacaa gctataaagc ctatagtcat
    10801 aatcaacttc
//
LOCUS       DAOSOX010000079        10621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6233_length_10621_cov_8.977475, whole genome shotgun sequence.
ACCESSION   DAOSOX010000079 DAOSOX010000000
VERSION     DAOSOX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_10621_cov_8.977475"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLX22_07040"
     CDS             complement(<1..934)
                     /locus_tag="PLX22_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT19692.1"
                     /translation="MNYRQNKKYYLKVVLTQFYSVPYYMVMVALLFCFSCSRFEWVRD
                     PYTLYVHLSAEPGHLNPITSTEAIASSINTYIYETLLDRDYDTLELKPLLASKWEISS
                     DKLHYRFYLKKNIRWSDGKPFTADDIVYSFHVIKDPKVANAPLKVYYIDVVSVKKISP
                     YIVEFTYSRPYFLALEICGSIPVVPKHIFDDGTDFNTHKNNRHPIGTGPYTFSKWETG
                     KRIELEINPLYWGKKPDIKKVVYKVIPEPSVALQMLKKGEIDVMSLRPIQWVRQTDSQ
                     HFKEAFYKFMYYQPYYSYIGWNAKNPLFNNKNVRK"
     gene            complement(977..2314)
                     /locus_tag="PLX22_07045"
     CDS             complement(977..2314)
                     /locus_tag="PLX22_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOT19693.1"
                     /translation="MIFLNEIRAWVWGAPLIILLLGTGIYLTILLKGLQFRTLVPSLY
                     LALIKRKEDGAHPGDISHFQALMTALSATVGTGNIAGVASAIAIGGPGALFWMWLTGL
                     FGMATKYSEAVLAVKYREVDALGTMSGGPMYYIEKGLKNKWLAFIFALFAACAAFGTG
                     NIIQSNSVAEAMHATFNVPSWVTGIFLLMITYLVIMGGIKSIAKAASAIVPFMILFYI
                     IVGTVILILFYKNIPHAFTLIFVHAFTPVAAAGGFAGAGVKMAMQMGVSRGLLSNESG
                     LGSSPIAAAAAKTDEPVRQALVSMTQTFIDTIIVCTFTGLVILVTGAWETGKTAAALT
                     EHAFSMGLPGTWGGIVVSISLIFFAYSTILGWSYYGEKAIEYIAGERAIKPYRLLWVI
                     IVFIGAVGRLDVVWAVTDIMNGLMAFPNLIALLGLSFVIKNETNHFFSKHTTI"
     gene            2497..3792
                     /gene="purB"
                     /locus_tag="PLX22_07050"
     CDS             2497..3792
                     /gene="purB"
                     /locus_tag="PLX22_07050"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT19694.1"
                     /translation="MIDRYSRKEISDIWTLENKFKIWLDIEIAACEANAQLGIIPQED
                     LKSIKEKARFTVARIEEIENQVHHDVIAFLTCVSEYVGPSSRYIHYGLTSSDIVDTAL
                     SIQMKQAGEIILNDINQLLAILKKLALNYKQTPCMGRSHGVHAEPTTFGTKMALYYHE
                     FLRNRKRIQDAIDDIAYGKLSGAVGTFSNIDPQVETIVCEKLGLKPDPISTQIIQRDR
                     HAYYMSAIAICAGTLDKLATEIRHLQRTEVREVEEPFQKGQKGSSAMPHKRNPVLCER
                     VSGLSRVIKSNMMVALDNMTLWHERDISHSSAERIILPDSTIALDYLLAKMIFILDNL
                     HVYPDAMMKTIEKTGGLFYSQALLLKLVEKGYTREEAYAIVQDIAMNVWHNGGSLKEL
                     SLNDTRINTKLSPEEIEEIFKLDNFLHNIDYIFKKAGLE"
     gene            complement(3793..5919)
                     /locus_tag="PLX22_07055"
     CDS             complement(3793..5919)
                     /locus_tag="PLX22_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19695.1"
                     /translation="MEAKNIYRQFQKYGSMVIVFNSIVFFIILAISFPVFYDLRIMVA
                     FIILTIIAFVSLFSYYMIMVQSILKIIDKDDEESRKKIFNYFKNYALQTMLLNIVYLV
                     VIYVPLFLYMYFFCGYTNIYYHFYVFFIIAFVFLFLGYNSMLVWYNRTYPLGRYGIPV
                     AVQRLRSKIVSIVLPVTLLTSVFLLVVFYQVDKSNVIKLTADHIYESLQNIKIDSKEL
                     FLQTIPRVISKNNGVVLVYDGNTINISNDEQYESKAISDIIKKGNQPEFLFHKTINFF
                     KNINRKQFNTILGVFNGEQSVLYGYNNISGNYAIVAIFSEQLLYKNVYQSIFFVTLSM
                     ILINFIIRYIINRRLVNLSRALDVAMPVLQKVNKGDLSGEITLIKSRDIMEDFIRGFI
                     GFRELITDFIKKSNELSEKVLLSSEALAFSGKSIKDEASQQAQNIEEVTSIIAELSSS
                     FFTIANDANKQRDILENLEKIVTNLNEAFTILKADAQQVIKAIEGIQNSAHQSETIVD
                     STTESTKQIASFFEKIMNVIGLIADIAEQVNLLSLNASIEAARAGEHGRGFAVVAEEI
                     SRLADRTAQNLKEISSIIHEGNSAMNSSMQMMEAMRKGLRSIVNDIEHSVTLFTGFIE
                     TINQRVKDFATIHEGIIGASEFSKGLSLSMSDLSDNTKKIVQSIENVNAVAGQFVSLS
                     ENLTSTASELETMAAELKKVIDRFSI"
     gene            complement(6226..6942)
                     /locus_tag="PLX22_07060"
     CDS             complement(6226..6942)
                     /locus_tag="PLX22_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19696.1"
                     /translation="MMQISLKKKFIIILLIAIVIGAIVTVDSYYNSKKKSIEKNYIPD
                     NYRAYLQKEERNISQLGSMSKQESLQYLQKIFGSLQYDNAHEESDYVPKQVAPALHEL
                     KEKNYELYELNNAIFLLKKKEGLPTIVWVPISDNQADIRDCHSMLENIAQLKERIDSG
                     KETYEERMQYYVLKKSFFENKLELMDYYLNLLNEEKTHYENDDNPERGDESIVWQIEE
                     IGRIKELLKKEIENINNQLH"
     gene            complement(7131..7730)
                     /locus_tag="PLX22_07065"
     CDS             complement(7131..7730)
                     /locus_tag="PLX22_07065"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOT19697.1"
                     /translation="MKSLKLAQLAVVVCVALLISVDMPALVENYQGDGNRLNISLVQK
                     GDVILMKGCKKMADLIGGLFPGYWHHAAIAESAGYYGIMIEAWTNGVRRLDTRSTLTV
                     DEAAIYRVNTSSTVKNNAVSFAAGKIGRPYDYGWLLWPGTKSTSGSTWYCSELAWAGY
                     KVNGVDIDANPGYHWMFWNNVAPGELADDGNTYQIARSN"
     gene            complement(7859..>10621)
                     /locus_tag="PLX22_07070"
     CDS             complement(7859..>10621)
                     /locus_tag="PLX22_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT19698.1"
                     /translation="MGKEISIKTLASIMNIDELELLSLLKSSSILDIIKIDIHPYEVN
                     FINFINDKLRDTYVASIDPERKKMIHKNIAEYFASNIDMYDTAIYDIIYHYINISEIE
                     KALQYIFDAVKKAKEIYGYQDIIKILKIIIDAMSVKNTQKEHEIYCDLILELSNLLIV
                     TGQLDEAKYYINQLNVNTLDDEHKLNLYSLYCDLHYKKGEWINCEKYARKGLKIIHEQ
                     LPDNKQVIFYIIKELLKALFSPLYTFIPLSSTNKQRKIKIFNFLEPLGMSYVLSNTLK
                     FLYIGLRSRNIAYRIGYSKQMCISLYARAGIAMIIALYPLSIFYLKKSRKVSKKIDDK
                     WLEAKTYELEGYCNEWQGNYTNGINNFNKAIEMFTKLGDVKELLMSLNGLEHCYYYTG
                     NYFKAESINQEYLNIAEKINDSYSITAAYIYFAQIEREKGNYEKSEKYAYDAFNVSKK
                     ENILFNQCSALNELGATAIMKKNYTAAVDFLINAQQIIADNSFIPQYVYPVYCNFADA
                     LIKLHQSSKNTIDDIHSKQLRHKMKKAIFTSIMKTFYLPTHFASSLRVAALYFFDVKN
                     KKLFSDILFWLSHIVGEKYHRKYEVAKTCYEYSQMLIDVGSNRVDDYIQKAFNYCTEI
                     GAHELHAKLNMILGYDEENRYKDFIEKSIYGELIAFEKNQLLNKKIIEYNSHNDYIKA
                     IVAILHELCKIVLAEGSIIGECSNSEFSLHHCCNINEHDAKNFYIKEYADYSLFNNFP
                     YIVTHNIDGIDFQLLYIPVQQQNLIIIFYSRYNFFTETIAREVLDFLLSIRKIVAENV
                     EKTKQKINKSTGETISYKLENKIQLAKDYIENNYQYNISKHDIATVVDLNADYFARLF
                     KQLTGENIGDYINRIRVEKASELLITTDKKIIEIAFDVGFEDLSTFNRNFFKFLKKSP
                     KTFRKQ"
BASE COUNT         3446 a         1947 c         1565 g         3663 t
ORIGIN      
        1 ctttgcgaac atttttattg ttaaacaggg gattttttgc attccagcca atataactat
       61 agtagggctg gtaatacatg aatttataaa atgcctcctt aaaatgttgt gaatcagtct
      121 gccgtaccca ttgaatgggc ctcagcgaca tgacatctat ctcacccttt tttagcatct
      181 gcaatgcaac gctgggttcg ggaattactt tataaacaac cttcttaata tcaggctttt
      241 taccccagta aaggggatta atctctaatt caatacgctt ccccgtttcc catttgctaa
      301 atgtatatgg tccggtacca atgggatgtc tgttattttt atgcgtgttg aaatcggtgc
      361 catcatcaaa gatatgctta ggaacaacag gtatactgcc gcatatctcc agtgctaaaa
      421 aatatggccg tgaataagta aactcaacaa tgtatggtga aattttttta acactgacta
      481 catcaatata atacaccttt aaaggagcat tggcaacctt tggatctttg atgacatgaa
      541 acgaatagac aatatcatcg gcggtaaaag gcttaccatc actccagcgt atattcttct
      601 ttaaataaaa gcggtagtgt agtttatcag atgatatctc ccattttgaa gctaatagcg
      661 gttttaactc taacgtatca taatccctgt ccagcagtgt ttcataaatg taggtattta
      721 ttgatgatgc aattgcctct gtcgatgtta taggattgag gtgtccaggc tcagcagaaa
      781 gatgaacgta gagcgtatat ggatcgcgca cccattcaaa acggctacat gaaaaacaaa
      841 acagcaatgc taccatcacc atataatagg gtacgctgta aaattgtgtt agcaccactt
      901 ttaaataata ttttttattt tggcgataat tcatggtatc aaacttttgg ttacaatcta
      961 tgtacagtat gcatggttat attgtagtat gtttgctaaa gaaatgattt gtttcatttt
     1021 ttataacaaa cgacaacccc aacaacgcta tgagattagg aaaagccatt aaaccgttca
     1081 taatatcggt aactgcccac acaacatcca gcctgccaac agcaccaata aatacaataa
     1141 ttacccacag taaacgataa ggctttattg ctctttctcc tgctatatat tctatagctt
     1201 tttcaccgta atatgaccat cctaagatag ttgaatatgc aaaaaatatc agagatatcg
     1261 aaacaacgat acctccccat gtgccgggta gccccatgct aaatgcatgc tcggttaacg
     1321 cagcggcagt ttttcccgtt tcccacgcac cggtaacaag aataaccagc cctgtaaaag
     1381 tacaaactat tatagtatca ataaaagtct gtgtcatgga aactagtgcc tgtcgtacgg
     1441 gttcatcggt ttttgctgca gcagcagcta tcggtgaact tcctaaacct gattcattgg
     1501 aaagcaatcc tcgtgaaaca cccatctgca tagccatttt aacacccgca cctgcaaaac
     1561 cgccagccgc tgccactgga gtaaatgcat gcacaaagat taatgtaaat gcatggggta
     1621 tatttttata aaatagtatc agtatgactg tgccaacaat gatataaaac agtatcataa
     1681 agggaactat cgccgaagct gcctttgcta tactttttat accgcccatg ataacaagat
     1741 aggttatcat taataaaaat attcctgtta cccatgatgg tacattaaat gtagcatgca
     1801 ttgcctcagc taccgaattg gactgtatta tgttaccggt tccaaacgct gcacaggcag
     1861 caaacagtgc aaaaataaaa gcaagccatt tgttctttaa tcctttttca atataataca
     1921 tgggaccacc tgacatggtt cccaatgcat caacctcacg atacttaacc gcaagcactg
     1981 cttcactgta ctttgttgcc atcccaaata aaccggtaag ccacatccag aacaatgcac
     2041 cagggccgcc aatagcaatt gccgaagcca caccagcgat attgccggtt ccaacggttg
     2101 ctgatagtgc agtcatgaga gcctgaaaat gtgatatatc gccggggtgc gctccatctt
     2161 cttttcgttt tatcaatgcc agatataatg acgggactaa agttctgaac tgcaatcctt
     2221 taagcagtat tgttaaataa ataccagtac ccagcaataa aataatgaga ggcgctcccc
     2281 agacccatgc tctaatttca ttcaaaaaaa tcatacttaa acctttatct tataatttag
     2341 tacattcaca atgttattgt tgctttatca ataatatatc cgtcaagcag taattcttga
     2401 tgattaacaa aatatacctt tttttacttg attaatttta atggccaaag agaattaact
     2461 ttttcatttt aattataaaa tgagcatggg aaatatatga ttgatcgtta ttcgcgaaaa
     2521 gaaatttcag atatctggac actggaaaac aaatttaaga tatggcttga cattgaaatt
     2581 gcagcctgtg aagcaaatgc acaattaggc ataattccac aggaagattt aaaaagcatt
     2641 aaagaaaaag ccaggtttac tgttgcacgt atcgaagaga ttgaaaacca ggtacaccat
     2701 gatgtcattg cttttttaac atgcgtttct gaatatgtag gcccttccag cagatatatt
     2761 cactatgggc ttacttcaag cgatattgtt gatactgctc tatccataca gatgaaacag
     2821 gctggcgaaa ttatcctcaa cgatataaat caacttcttg ctatactaaa aaaattagca
     2881 ttaaactata aacaaacccc atgtatggga cgttcacatg gcgtccatgc agaacctact
     2941 acattcggga caaagatggc attgtattat catgaatttt tgcgcaaccg caaacgaatt
     3001 caggacgcca ttgatgatat cgcctatgga aaactttctg gcgctgtggg aacattcagc
     3061 aatattgatc cgcaagttga aacgatagta tgtgaaaaat taggcttgaa accagatccc
     3121 atatcaacgc agatcattca gcgcgatagg catgcatact acatgtcagc cattgcaatt
     3181 tgtgccggca cactggataa acttgcaaca gaaatccgac atcttcagcg tacagaggtt
     3241 cgcgaggtag aagagccttt ccagaaaggg caaaaggggt cttcagcaat gccgcataaa
     3301 cggaatcctg tattatgtga acgtgtttca gggctatcac gggttataaa atccaacatg
     3361 atggttgcgc tggataatat gaccctgtgg catgaaaggg atatttcaca ctcatcagcc
     3421 gagcgtatta tactgcccga tagtactata gcactggatt acctgcttgc gaagatgatt
     3481 ttcatccttg ataatttaca tgtttaccct gacgccatga tgaaaacaat agaaaaaacc
     3541 ggggggctat tctattcaca ggctctgctt ttaaaattag tagaaaaagg atatacccgt
     3601 gaagaagcgt atgcaattgt tcaggatatt gcgatgaatg tgtggcataa tggcggttca
     3661 ttgaaggagc tatcgcttaa tgatacaaga ataaatacaa agttatcccc ggaggaaatt
     3721 gaagaaattt ttaaactgga taactttttg cataatattg attatatatt taaaaaagct
     3781 gggcttgaat aactaaatac taaaacgatc aataaccttt tttaactctg cagccattgt
     3841 ttctagttct gatgctgtgc tggtaagatt ttctgaaagg ctaacaaatt gccctgctac
     3901 cgcatttaca ttttcaatcg actgtactat ctttttagta ttatcactta aatcagacat
     3961 cgatagcgac agtccttttg aaaattcact tgctccaatt ataccttcat gaattgttgc
     4021 aaaatccttt acacgctgat taatggtttc tataaatcca gtaaataatg taacactgtg
     4081 ctcaatatca ttgacaattg aacgtaaacc tttacgcatt gcttccatca tctgcatact
     4141 gctgttcatt gcgctgttac cttcatgtat tatgcttgat atttctttaa ggttctgagc
     4201 agttctgtca gctaaccgtg atatttcctc tgctacaact gcaaatcccc tgccatgctc
     4261 tccagcacgt gctgcttcaa tggaagcatt caaagataat agattaacct gttcagcaat
     4321 atctgcaata agtcctataa cattcataat cttttcaaag aacgacgcaa tctgttttgt
     4381 tgattcggta gtactatcaa caatagtttc actttggtga gcgctgtttt gaatgccttc
     4441 tattgctttg attacctgct gtgcatcggc ttttaaaata gtaaaagctt cattgagatt
     4501 agtaacaatt ttttctaagt tttccagaat atcacgctgt ttatttgcat catttgcaat
     4561 agtaaaaaat gagcttgata gttctgcaat aatagaggta acctcttcaa tattttgtgc
     4621 ttgttgagat gcttcatcct tgattgattt tcccgaaaaa gccaatgcct cggaggataa
     4681 gagtaccttt tcactcaatt cattggattt tttaatgaag tctgttatca attctctgaa
     4741 ccctataaaa cctcgtataa aatcttccat gatatcacgt gattttatta atgtaatttc
     4801 accacttaga tcccctttat tgactttttg caataccggc atcgcaacat caagcgctcg
     4861 tgaaagattt acaagtcgtc tgttgattat atagcgaata ataaaattaa tgagtatcat
     4921 actcagggtt acaaaaaata tactttgata aacattttta tacagtaact gttcagaaaa
     4981 tattgcaact atagcataat tacctgaaat attattatat ccatatagta ctgactgttc
     5041 accattaaaa actcccagta tagtgttaaa ttgctttcta ttaatatttt taaaaaaatt
     5101 gatggtttta tggaataaaa attcaggttg attgcctttt tttattatat ctgatatggc
     5161 ttttgattca tattgctcgt cattacttat attaatagta tttccatcat aaaccaatac
     5221 aactccatta tttttagaaa ttacacgtgg tatagtttgt aaaaataatt ctttagaatc
     5281 tattttaata ttttgtaaac tttcataaat atgatctgct gttaatttta taacattgga
     5341 cttatcaact tggtaaaaca caactaataa aaaaacagag gttaaaagtg tcacaggcaa
     5401 cacaatactt acaattttac tgcgtagccg ctgaactgca acaggtatgc catagcgacc
     5461 taaaggataa gtacggttgt accaaaccag catcgaatta tatcccaaaa atagaaaaac
     5521 aaatgctatg ataaaaaaga cataaaaatg gtaatatata ttggtatagc cacagaaaaa
     5581 atacatatat aaaaacaatg gaacataaat taccactaaa tatactatat ttaaaagcat
     5641 ggtttgcaat gcataatttt taaaataatt aaatatcttt ttcctggatt cttcatcatc
     5701 tttatcaata atttttaaaa tggattgtac cattatcata taatatgaaa ataatgatac
     5761 aaaagctatt attgttaaaa ttataaatgc taccatgata cgtagatcat aaaaaacggg
     5821 aaatgaaata gctaaaatta taaaaaatac aattgaattg aacacaataa ccattgatcc
     5881 atatttttga aactgacgat aaatattttt agcttccata tattaatatt tcctgtacaa
     5941 ttattatgat cggtatcaat taactaataa agtataataa cattatttgc agaaaatcaa
     6001 ttcttttttt atattaattt tgattttttt aatgcagtgt accatgatca ttgttaatat
     6061 aagatttgag tataaagcat ctctcagaaa aataaaaaat atttcgttat actgtacata
     6121 gatattgctg ttattcccta tctgcatact tgtagtgatg gagtagtgat ggaattgata
     6181 gtatctatga gaatagcatt attgcacata gttattgatg aatgtctaat gtagctggtt
     6241 attgatattt tcaatctctt tttttaaaag ctcttttatc cttccaatct cttcaatctg
     6301 ccatactatg ctttcatccc cacgttcagg attgtcatca ttttcatagt gtgttttctc
     6361 ttcattcaat agatttaagt aataatccat gagttctaac ttattttcaa aaaaactttt
     6421 ttttaataca taatactgca tcctttcctc ataagtttct tttccactgt ctatcctttc
     6481 ttttaactgt gcaatattct caagcatgct atggcagtca cgtatatctg cttgattatc
     6541 agagattgga acccacacaa tagtaggcag cccttctttt tttttaagta aaaatattgc
     6601 attatttagt tcatataact cataattttt ttcttttagt tcatgtaacg ctggtgctac
     6661 ctgtttggga acataatcgc tctcctcatg tgcattatca tactgtaagc ttccaaaaat
     6721 cttttgaaga tactgcaggg attcttgttt tgacattgat cctaattggg aaatatttct
     6781 ctcttctttt tgtaaatatg cacgatagtt atcgggaata tagttttttt caatggattt
     6841 ttttttactg ttataatatg aatccacagt aactatcgcc ccgataacta ttgctatcaa
     6901 taatattata ataaacttct ttttaagaga aatctgcatc attgcaccat tacatacttt
     6961 atttttttgc aacatcgata gttacttgcg taagacatac aataattaca atgccacctt
     7021 caagaaaatg tatctttatt cttaaagatg gcattgataa aaactaccct tcctccttgc
     7081 agtacctctc tgctatatct atcattcttc aaccatagta catcaataca tcaattagac
     7141 cgtgcaattt gataggtatt accatcatca gctaattcac caggtgctac attattccag
     7201 aacatccagt gataaccagg atttgcatca atatcaactc cattaacctt atatcctgcc
     7261 catgctaatt ctgaacaata ccatgtagaa ccacttgtac tttttgtacc aggccatagc
     7321 agccaaccat aatcatatgg cctacctatc tttcccgctg caaagcttac tgcattgttc
     7381 ttgaccgtac tggatgtgtt aactctataa attgctgcct catcaactgt taacgttgat
     7441 ctggtatcca atcggcgtac accatttgtc catgcctcaa tcattatacc gtaatagcct
     7501 gccgattctg caattgctgc atgatgccag taacccggga ataaaccgcc aattaaatct
     7561 gccatctttt tgcaaccttt cattaaaatg acatcgcctt tttgcactaa actgatatta
     7621 agtctattgc catcaccctg ataattctcc accaatgcag gcatgtcaac agaaataaga
     7681 agtgcaacac aaacaacaac agccaattgt gctaatttta agcttttcat tattcaaacc
     7741 tcctgtaaat acatgaaata tataaaatac cttctggttg gtattttata ttaataaacc
     7801 agatatataa ttatggttca atgcataact agatatatct atttttgcat atattttatt
     7861 attgttttct aaatgtttta ggggattttt tcaaaaattt aaaaaaatta cggttaaaag
     7921 tacttaaatc ctcaaatcct acatcaaaag ctatttctat tatcttttta tctgtcgtta
     7981 ttaaaagttc agaagccttt tctactctta ttctatttat atagtcccct atattctcac
     8041 ccgttaattg cttaaataag cgtgcgaagt agtcagcatt taaatctaca acagtggcaa
     8101 tatcatgctt tgaaatatta tactgataat tattttctat gtaatctttt gctaattgaa
     8161 ttttattttc aagtttatac gatatcgttt ctccagtaga tttatttatt ttttgttttg
     8221 ttttttcaac attttcagct acaatttttc tgattgataa taggaaatcc aaaacttctc
     8281 tggcgatagt ttccgtaaaa aaattatacc gtgaataaaa gataattatt agattttgtt
     8341 gttgaacagg aatatacagc aactggaaat cgataccatc tatgttatgc gtaacgatat
     8401 acggaaaatt attaaatagc gaataatctg catattcttt tatataaaag ttttttgcat
     8461 catgttcatt aatgttacaa cagtgatgca atgaaaattc actattactg cattctccaa
     8521 taatacttcc ttcggctaat actattttgc ataattcatg taatatggct actatagcct
     8581 ttatataatc gttgtgactg ttatactcaa tgattttttt atttaataat tgattttttt
     8641 caaatgctat taattcacca tatattgatt tttctataaa atctttatat ctattttctt
     8701 catcatagcc aagtatcatg tttaattttg catgtaattc atgagcacct atctcagtac
     8761 agtaattgaa agccttctga atataatcat caaccctgtt gctaccaaca tcaataagca
     8821 tttgcgaata ttcataacaa gtttttgcaa cctcgtactt tctatggtac ttttcgccaa
     8881 ctatatgact taaccagaat agtatatctg aaaataattt tttatttttc acatcaaaga
     8941 aatataaagc tgcaactcgt aatgaactgg caaaatgagt tggtaaataa aaagttttca
     9001 ttatggacgt aaaaattgct tttttcattt tatgacgtaa ctgtttacta tgaatatcat
     9061 ctattgtgtt ttttgaactt tgatgcaatt ttatcaacgc atcagcaaaa ttacaatata
     9121 caggatatac atactgaggt ataaaactat tgtctgctat aatctgttga gcatttatca
     9181 gaaaatcaac ggctgccgtg taattttttt tcattattgc tgtagcacca agttcattca
     9241 acgctgaaca ttgattaaat agaatatttt cctttttact tacattgaat gcatcgtatg
     9301 catacttttc cgatttttca taattacctt tttcacgctc aatctgtgca aaataaatgt
     9361 aagcagcggt aatggaataa ctatcattaa ttttttctgc aatatttaaa tattcttgat
     9421 ttattgattc agctttaaaa taatttcctg tataatagta acaatgttcc aatccgttta
     9481 atgacatcag taattccttt acatccccaa gctttgtaaa catttcaata gctttattaa
     9541 aattatttat tccattagta tagtttccct gccattcatt gcaataacct tcaagctcat
     9601 aagtttttgc ctccagccat ttatcatcta tttttttact aacctttctg gattttttta
     9661 aatagaatat agacaatgga tataacgcaa taatcattgc aatacctgca cgtgcatata
     9721 aactaataca catttgtttt gaatatccaa tcctataagc tatgtttctg ctacgcaatc
     9781 ctatatataa aaactttaag gtattgctca acacatagct catccctaat ggttctaaaa
     9841 aattaaatat ttttattttt cgttgtttat ttgttgatga taagggaatg aaggtatata
     9901 atggggaaaa taatgctttt aacaattctt ttattatata aaaaataacc tgtttattat
     9961 caggcaattg ttcatggata atctttaatc cttttctggc atatttttcg caattgatcc
    10021 attctccttt tttataatgc aaatcacaat ataatgaata aagattaagc ttgtgttcat
    10081 catccaatgt attaacattt aattggttaa tgtaatattt tgcttcatca agctgacccg
    10141 ttactattaa taaatttgac aattctaaaa tcaaatcaca atatatttca tgttcttttt
    10201 gagtattttt taccgacatt gcatcaataa taatcttgag aattttgatt atatcctgat
    10261 acccataaat ttcttttgcc ttttttaccg catcaaatat atactgtaga gctttttcaa
    10321 tttcgctgat gtttatatag tggtaaatga tatcataaat tgcagtatca tacatatcga
    10381 tattactggc aaaatattca gcaatatttt tatgtatcat ctttttcctt tccgggtcaa
    10441 ttgatgctac ataggtatct cgtaacttat cattgataaa attgataaaa ttaacttcgt
    10501 aaggatgaat atcaattttt ataatatcaa gtatagatga agactttaat aatgatagca
    10561 gttctaattc atcaatattc atgatagatg ctaatgtttt tattgatatt tctttcccca
    10621 t
//
LOCUS       DAOSOX010000080        10618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6235_length_10618_cov_9.083215, whole genome shotgun sequence.
ACCESSION   DAOSOX010000080 DAOSOX010000000
VERSION     DAOSOX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_10618_cov_9.083215"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..441
                     /gene="trxA"
                     /locus_tag="PLX22_07075"
     CDS             127..441
                     /gene="trxA"
                     /locus_tag="PLX22_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT19699.1"
                     /translation="MGTIFEVNDTNFNAEVLECAGKVLVDFWAPWCGPCRMQTPILEK
                     LAQDPETKTKIVKVNTDNNPETAQRYGISSIPTLILFDNGKEIERMIGVQPENTLKKK
                     LS"
     gene            445..894
                     /gene="dtd"
                     /locus_tag="PLX22_07080"
     CDS             445..894
                     /gene="dtd"
                     /locus_tag="PLX22_07080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT19700.1"
                     /translation="MRAVIQRVLQASVEVDGNSIASINKGLAVLAGFAPSDTTKDLEY
                     ICQKIIGIRIFDDASFFMNLSLSDVHGGLLLIPQFTLYGDARKGRRPSFSDAMKPETA
                     SEYFDSFVAMCKAAHPHVVTGKFKAHMKVILVNDGPVTILLDSSKLF"
     gene            complement(897..1424)
                     /locus_tag="PLX22_07085"
     CDS             complement(897..1424)
                     /locus_tag="PLX22_07085"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19701.1"
                     /translation="MRNIYTTVLLMVSMALASGAIARNNDEAASQYNGAGLYCLESGN
                     YQKASEYFIDAIQLNPANKFYYNNCAVAWMNMGRYEDAMDMLQIALAIDPHYVKALTN
                     MAIVCFHLLKFSRAYYYYSQARTADSIYTKERFQMAKVIAGVKRVYARHPDNNDLQQI
                     LNQLEILQQRKKDLP"
     gene            1626..>2525
                     /locus_tag="PLX22_07090"
     CDS             1626..>2525
                     /locus_tag="PLX22_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT19702.1"
                     /translation="MDLLQKLNEEQYNAVTYSDGPLLILAGAGSGKTRVITYRIAHLI
                     KVAGIPPYAIVAVTFTNKAAEEMRNRIKGLIGPIGESVMIKTFHALAVYILRRHGDAI
                     GIAPNFTIYDSRDQESLIKDILHDIHIDPKAVKPSMIAEKISSIKESSHYIQGGDITL
                     LLPKYHSFNFQEIFTSYQQKLKQSNALDFSDLLIYTVQLLRNSQVLTTLQSRWRYFMV
                     DEYQDTNFAQYLIAKYLASQTKNICVVGDDDQSIYSWRGADIRNILNFEKDYPDAKVI
                     TLHKNYRSTAPILQAASYVIKNNY"
     assembly_gap    2526..2625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2626..3324
                     /locus_tag="PLX22_07095"
     CDS             <2626..3324
                     /locus_tag="PLX22_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT19703.1"
                     /translation="RGRLENIDAFVNSIVEYEHLHPEATLFEFLQDISLLSPADETNA
                     TAPEGNNAITLMTVHNAKGLEFPVVFLTGMEEGLFPHANAIDSDEGIEEERRLCYVGI
                     TRAKDRLVITSAELRRSWGTTQFKEASRFISELPPALCQFQHYSSSYNNWDMPKTNNH
                     NNYRNIDNYTATALHNNDSQYTVNQRVKHPSFGVGKITAIQGKGDNVKLTIQFLNGFT
                     KTFLEKYTPLEPLS"
     gene            3398..>3684
                     /locus_tag="PLX22_07100"
     CDS             3398..>3684
                     /locus_tag="PLX22_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19704.1"
                     /translation="MEKTHRVSSTIMSNIPVLILEGDITSEADADIMNEYRKLKEKQA
                     PFYCILDFTKTKYINSAGIAILINIIQDFGDFGGKVIFTGLSPHFNKVMDIV"
     assembly_gap    3685..3784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3785..5127)
                     /locus_tag="PLX22_07105"
     CDS             complement(<3785..5127)
                     /locus_tag="PLX22_07105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT19705.1"
                     /translation="MDINEIIHTVFTDGVLLNYIPDYEYRAIQEQMADAIITAWYNEK
                     HCIIEAGTGTGKTLGYLLPAALFAMHEDVKVAIVTETKALQQQLMLNDLPIIANAIRD
                     ITGTSLTYALCLGSNNYPCYRRLQNAIDTGSFDKSKEKEIYHVHEVVTKQPALTRFDI
                     MAGESLWHTICRESQLCPIHTCKYKNRCSYILAKKNWQEANILVMNHYLFFSNIAASK
                     SLVPDFKVVVFDEAHSIERIASNQLGFAINTEQLKETIEHIIRRRKGGALIERVLTYD
                     MISEAMEVFDNALRQVQLSKKILNDIVKNLQTLRLRNPLAECLPLLKALEEVLRLFEK
                     AEPETMDEDLRLEYEYHKASIMDTWHDLGMAVFMDEEKYVYWLEKEKNSQVNIFICGQ
                     PLDVASIIHKEVLDYYDHCCFVSGTLSVNNSFEYFKQSIGCDNADTLILSSPFNFT"
     gene            complement(5191..>6472)
                     /locus_tag="PLX22_07110"
     CDS             complement(5191..>6472)
                     /locus_tag="PLX22_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOT19706.1"
                     /translation="EGDIKSDEIFSKEKDLDESITIDLDTLDIPIEESTVIEQAEMPL
                     TEPDILEKGELPEEEIGFDDILVSEEPARKPKKTISPMGKEKEKLDDITLDLDSLDIQ
                     LDEHAETLPGEQIIDEDEKLTLEDAGITIDELIEEQKKEPIEEEEELKLSLDEIEPGL
                     TADDLHKELTESELETIIASEENFDTYAIKDEMELPEVDLDKYEMGAGEVEQQKAEYH
                     HAENEDFDSVPAGFVNLTIDYSLSYSRSNAILRLLVVYLLSLLPHILVCAVYSIVSMV
                     LGFFNWVVILFTGQFMDDFADIQQNTLRYWLSIVACAAQITEDRPVYAGRPLVEHSLQ
                     LDVVYPSSPSRLLALLRISLIGIIIALLPHLLLYIILTMGVLLLVPIGLMSIVAIKHW
                     PTLLFDFMYRYMRYAARIWAYAAGLVDKYPSFIF"
     assembly_gap    6473..6572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6573..6757)
                     /locus_tag="PLX22_07115"
     CDS             complement(<6573..6757)
                     /locus_tag="PLX22_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOT19707.1"
                     /translation="MIVRCSECNSAYQVDDEKVQNKRFAFTCPRCKAHVIVDNRAEGY
                     DASFDDMMLAQDTGKPHT"
     gene            complement(6775..7095)
                     /locus_tag="PLX22_07120"
     CDS             complement(6775..7095)
                     /locus_tag="PLX22_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19708.1"
                     /translation="MQSKNAFEYIPEYNLVVLFVLILCTAGIYYFWWIARVSRVFYDN
                     PVTNILLIILTAGIWLVYITIKYMQKSEMLNDRQMPWYMILFLPIGFLIIQHNINERY
                     FTSQ"
     gene            7460..10414
                     /locus_tag="PLX22_07125"
     CDS             7460..10414
                     /locus_tag="PLX22_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19709.1"
                     /translation="MHIQEFNYSNTAQRRILLLSLFFSIVTLSSYAIMSTLFLTSIVL
                     YQAVWKYILTLSLSGILTGIIIGTIIHKKISFSRMLLILLQIVISCLAIAPGIASYFD
                     NGIETIYLAIAQKFSLLFYCLIFLLFTLEGIKSAYFLKTSIGDYIDNKNPALFAISVA
                     LFSIVFSFYLSLLLYSHMEYLWIYFVFPLLSLIFIIFLNLPFCPPTIISRTAITKPDD
                     ISTRDDVLFTYSNISIISIFTTLILITIYSQHGIFIATTMYTISAIIGGFLIGMFLSA
                     LLKKYNTVFIYGEMLYPLLFLAFIIISHTASPSLYSSLLAAAPFSIFAGITFGTSLKH
                     ILIKNNHSTRYTIILFSFFLIPLPIIIAFIWIELTYFWFFAVTYILSIITIVFPGLYL
                     LQNFKRSVPSIIYCIVSLLFIPAIIVTHTYFKLPLSDSLVNAYSRGYEDVVSSNFNSS
                     FITKPGVAYINDYVACRITDATARDLKRSITFISLFANNHNTIIIDGYHKFFTNSLFH
                     LLNSPIVLDYISDRIVDYQKPPISDVHFATIHTSIIQGLKQKKGLIVDIPNTFDHYMY
                     PFRFSDAYIAIIKEHVDSYYFTIIDITHIAPNYLYSLTNALKKYFLHTVVFVSGNHLT
                     IGATNNSNGFTLGASDIQSLENLIQTTEVANLFVNAEHALSYMVSNTIELIMPRNGVF
                     TTFTLPITKPAQQLFINPFVEKVITTNDAVVQIIDNQYYYANTIGLRMLYAKQINQLI
                     KQSEYYGLQQNYESEMQTLMQLRRIAEYTPPLRQYITNIISFKEKLYETIALDYEKQK
                     QWDRAISLYNAMLAVNPNNFTANYRIGIINATIQNLENAFKYLNNAMRMAPNNPDVLY
                     QLGILLVSQGKFNEALQYLTQAHQMNMASASLFFNIGLCYESLKNLEEAKRYYEKAKF
                     IDPNDATIQSSLERVSEQKVQQSNQNADTRTNQIEEEKGESFPLPINQSAYDVRLKEN
                     ELKDQNTSSIQQ"
BASE COUNT         3331 a         2061 c         1688 g         3238 t
ORIGIN      
        1 ctattgacat ttcctgactg catcgtgtat ttttagcata attgtataaa caaaaaaata
       61 aacttctgga attattgaaa atcatatgct atgatagtat cttactattt ccttgtggag
      121 gtaactatgg gcactatttt tgaagtaaac gacactaatt ttaacgccga ggtgttagaa
      181 tgtgccggta aagtgctggt tgatttctgg gccccatggt gtggtccctg ccgcatgcaa
      241 acgccaatac tggaaaaatt agcgcaggat cccgaaacaa aaacaaagat tgtaaaagta
      301 aataccgata acaatcctga aacagcccaa cggtatggta tatcttctat cccaacactt
      361 atactttttg ataatggcaa agagatagag cgcatgattg gcgtacagcc tgaaaacact
      421 ttaaagaaaa aactttcata aacaatgcgt gctgttatac agcgtgtttt acaggcttcc
      481 gtggaagttg atggtaatag catagcatcc atcaataaag gtttggcagt acttgcaggg
      541 tttgcaccat cagacacaac aaaagattta gaatatatct gtcaaaaaat tataggcatc
      601 cgaatttttg atgatgccag cttctttatg aacctgtcat tatctgatgt tcacggcggg
      661 cttttactta tcccccaatt tacactttac ggtgatgccc gcaaaggacg ccgtccttca
      721 ttttctgacg ccatgaaacc tgaaacagca tcagaatatt ttgattcgtt tgtagcaatg
      781 tgtaaagcag cacatcccca tgttgtaacc ggcaaattta aagctcatat gaaggttata
      841 cttgttaacg atggtccggt aaccatactt ttagacagct caaaattatt ttgaatctat
      901 ggcaaatctt ttttacgttg ttgtaatatc tcaagctgat tcagaatctg ttgtaaatca
      961 ttgttatcgg gatgtctggc ataaaccctt ttaacaccag cgattacctt tgccatctgg
     1021 aatcgttctt ttgtatagat actatcggct gtccttgcct gtgaataata ataataggca
     1081 cgtgaaaact tcaaaaggtg aaaacaaaca atagccatat tggtcaatgc ttttacatag
     1141 tgaggatcaa tagcaagcgc tatttgcaac atatccatgg catcctcata ccttcccatg
     1201 ttcatccatg caacagcgca attgttgtag taaaatttat tggcaggatt taattgtatt
     1261 gcatcaataa aatattcaga ggctttctga taattacctg attctaaaca atataagcct
     1321 gcaccattgt actggctggc agcttcatca ttattccggg cgatagctcc tgaagctaat
     1381 gccatgctaa ccatcagcaa cactgtggta tatatattcc tcatcgcatc ctctttgctt
     1441 ttaaatcggt tgtatatttt atagaatatg taatgaaaaa atatagaata atacaagact
     1501 attttttttt caattattaa tattaatttc attacacagg ttataatttt tcttttttat
     1561 cttgcagtat ttttattatt attaattatg tcatcgtcat actacaatcc gtttgaatac
     1621 actctatgga cttactacaa aaacttaacg aagaacaata taatgccgtt acgtatagtg
     1681 atggcccgct gttaatcctg gcaggagctg gttcgggcaa aacacgggtt attacctacc
     1741 ggatagctca tttaataaaa gttgcaggca taccacccta tgccattgtt gcagtaacct
     1801 tcaccaataa agctgctgaa gagatgcgga accgcatcaa aggactgata ggtcccattg
     1861 gcgaaagtgt aatgattaag accttccacg cattagcagt gtatatactg cgacgccatg
     1921 gtgatgcaat aggcattgct cccaatttta ccatttatga cagccgtgac caggaatcac
     1981 tgattaaaga tatattacat gatatacaca tagacccaaa agcggtaaaa ccttcgatga
     2041 ttgctgaaaa aatttccagc ataaaggaaa gcagccatta tatacagggt ggcgatataa
     2101 cattgctatt gccaaaatat cattcattca actttcagga gatatttacc agctatcagc
     2161 aaaaactgaa acaatccaat gctctggatt tcagtgacct tttaatctat acagtgcagt
     2221 tgctgcgcaa cagccaggtg cttacaacat tacaatcgcg ctggcgctac ttcatggttg
     2281 atgagtatca ggataccaac tttgcgcagt atctcattgc taaatacctg gcatcacaaa
     2341 caaaaaatat atgtgtggtg ggcgacgatg accagtcaat ctactcatgg cgtggagctg
     2401 acatccgcaa tatcttaaac tttgagaaag actatcctga tgcaaaggta attaccctcc
     2461 acaaaaacta tcgctcaacc gcccctattt tgcaagcagc ttcatacgtt attaaaaata
     2521 attacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgggg aaggcttgaa
     2641 aatatcgatg catttgttaa tagcattgtt gaatatgagc acctccatcc tgaagcaaca
     2701 ctgtttgaat ttttacagga tatttcactg ctttcacctg ctgacgaaac caatgcaacc
     2761 gctccagagg gaaacaacgc cattactctt atgactgttc acaacgccaa gggattggaa
     2821 tttcctgtag tttttttaac agggatggaa gaggggctgt tccctcacgc caatgcaatt
     2881 gacagcgatg aagggataga agaagagcga cgtttgtgct atgttggcat aacacgggca
     2941 aaagaccgcc tggttataac cagcgccgaa ctgcggagaa gctggggaac aacacagttt
     3001 aaagaagcat cgcgttttat aagcgagctc ccaccagcat tatgccagtt tcaacattat
     3061 agctcatcat acaataattg ggatatgcca aaaacaaaca accacaacaa ctatcgcaat
     3121 atcgataatt acactgccac agctttacat aataacgact ctcaatatac agtaaaccag
     3181 agggttaaac acccttcatt tggtgtagga aaaattactg ctatacaggg aaagggtgat
     3241 aatgttaaac tgaccataca atttcttaac ggatttacta aaacatttct ggaaaagtat
     3301 acccccctag aaccattgtc atgatttttt ttcttgacaa tttatgctca cgaatacatg
     3361 aattataaac agaagaatac ttccaaaggt gtggattatg gaaaaaactc atcgggtatc
     3421 gtctacaata atgagcaata tccctgtatt gattttagaa ggagatataa cctctgaggc
     3481 tgatgctgat attatgaatg aataccggaa attgaaagaa aaacaggcgc cattctattg
     3541 tattcttgat tttaccaaaa caaagtacat caattctgcc gggattgcaa ttttaataaa
     3601 tatcatacag gattttggtg actttggcgg taaggtaata tttaccggat tatcaccaca
     3661 ttttaacaag gttatggata ttgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnngtaaaa ttaaagggcg acgataaaat taaggtatct gcattatcac atccaatact
     3841 ttgtttaaaa tattcaaatg aattgttgac gctcaacgtt cccgaaacaa aacaacaatg
     3901 gtcataataa tccaaaacct ctttatgtat tatcgaggca acatccagcg gctgcccaca
     3961 aatgaaaata ttaacctgtg aatttttttc tttttccagc cagtacacat acttttcttc
     4021 atccataaat acagccatcc ccagatcatg ccatgtgtcc attatcgaag ctttgtgata
     4081 ttcatattct aatcgtaaat cctcatccat tgtttctggt tcagcttttt caaataaccg
     4141 taaaacctcc tcaagagctt tgagcaaggg aagacattcc gcaagggggt tccgcaatcg
     4201 cagtgtttgt aaatttttta caatgtcatt gagtattttt ttagatagtt gaacctgacg
     4261 cagtgcattg tcaaaaactt ccattgcttc actgatcata tcatacgtta aaaccctttc
     4321 aattaatgca cctccttttc tgcgtctgat aatgtgctct attgtttctt tgagttgctc
     4381 agtattaatt gcaaatccca gttgattaga agcaatccgt tcaatggaat gcgcttcatc
     4441 aaacaccaca accttaaaat ccggcaccaa tgactttgaa gcagcaatat tggagaaaaa
     4501 aagataatga ttcataacaa ggatgtttgc ttcctgccag ttttttttag ctaaaatata
     4561 tgaacatcta tttttatatt tacatgtatg gatagggcat agctgtgatt ctcgacagat
     4621 agtatgccac aaggattcac ctgccataat atcaaaccgc gtcagcgcag gttgctttgt
     4681 tactacttca tgtacatggt atatctcttt ttccttactt ttgtcaaaac ttcccgtatc
     4741 gattgcattc tgcaaacggc gatagcaggg ataattgttg ctgcctaaac acagggcata
     4801 ggtaagagaa gtacctgtta tatcgcgtat ggcattggca atgatgggta aatcattaag
     4861 cattaattgc tgctgcaaag cttttgtttc ggtaactatc gccactttaa cgtcttcatg
     4921 cattgcaaaa agagcagcag gtaaaagata ccctaacgtt ttacctgtac ccgtacctgc
     4981 ctcaatgata caatgctttt cattatacca tgcagtgatt attgcatcag ccatctgttc
     5041 ctgaattgcg cggtactcat aatcaggaat atagttcaaa agcacgccgt cagtaaatac
     5101 agtatgtata atttcattaa tgtccataaa caactgtgta tgtatttaat taattgaggt
     5161 aatgtatata actgggatat tttatgtcta ttaaaaaata aacgatggat atttatcaac
     5221 aagaccggct gcgtatgccc atatccgcgc agcataccgc atgtaccgat acatgaaatc
     5281 aaacaatagt gttggccagt gctttattgc cacaattgac attaatccta taggaacaag
     5341 taacaaaact cccattgtta aaataatata cagcaacaga tgaggcaaca atgctatgat
     5401 aatgccaatc aatgaaatgc gcaataatgc cagaagcctc gacggagatg atgggtacac
     5461 tacatcaagc tgcaggctat gttcaaccaa tggacgtcct gcatataccg gacggtcttc
     5521 ggttatctgt gcagcacagg ctacaataga taaccaatat cgcaatgtgt tttgctgtat
     5581 atcggcaaaa tcatccataa attgtccggt aaacagtata acaacccaat taaaaaatcc
     5641 caataccatg gaaacaatgc tatacactgc acacacaagg atatggggaa gtaatgataa
     5701 aagataaaca acaagaagtc gtagaattgc atttgacctt gaatacgaca atgagtaatc
     5761 aatagttaaa tttacaaaac cagcaggtac ggaatcaaag tcttcatttt ctgcatggtg
     5821 atattcagct ttttgctgtt caacttcacc tgctcccatc tcgtatttat caagatcaac
     5881 ctcgggcaat tccatttcat ctttgatagc atacgtatca aaattttcct cactggctat
     5941 tatcgtttca agctcggatt cggtaagttc tttatggaga tcatcggcag ttaatcctgg
     6001 ttctatctca tcaagtgaaa gttttagttc ctcttcttct tcaatgggtt cctttttttg
     6061 ttcttcaatt aattcatcta tagtgatgcc tgcatcttca agcgttaatt tttcatcttc
     6121 atcaatgatc tgttcacccg gcaaggtttc cgcatgttca tcaagctgaa tatcaagtga
     6181 atccagatca agagtaatat catcaagctt ttctttttct tttcccatcg gagaaatggt
     6241 tttttttggt ttacgagcag gctcttctga cacaagtata tcatcaaatc caatctcttc
     6301 ctcaggcaac tctccttttt caagaatatc tggttcagtc aaaggcattt cagcctgttc
     6361 aattacagta gattcttcga ttggtatatc aagggtgtca agatcaatag taatgctttc
     6421 atcaaggtct ttttctttag aaaagatctc atctgattta atgtcgcctt cannnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtatgtgg tttgccagta tcttgagcca
     6601 gcatcatatc atcaaagctt gcatcataac cctctgcacg attatcaaca ataacatgag
     6661 ccttgcatct ggggcacgta aatgcaaacc gtttattttg caccttttca tcatcaacct
     6721 gataggctga attgcattcg ctgcatcgta caatcattga ttgctcctga tggtttattg
     6781 actggtaaag tatcgctcat taatattatg ctgaataatc aaaaacccta taggtaaaaa
     6841 caaaatcata taccatggca tctgcctgtc attaagcatc tcagatttct gcatatattt
     6901 tatggtaata tagacaagcc atatccctgc tgttaaaata atcagtaaaa tgtttgttac
     6961 agggttgtca taaaataccc tgctcaccct ggcaatccac cagaaataat aaatccctgc
     7021 cgtacacagt attaaaacaa agagcacaac cagattatat tcagggatat attcaaaagc
     7081 attttttgac tgcatataag ctcccttttt aacttttact gtcatagaga atacagttat
     7141 agtaaaataa ctaaaatcat gcaacaaaaa aagtctaaaa gtatctatat ataatttacg
     7201 gtaaaatagt gaaaaaatct taataattat tcaatattca tacattaaat taatgtttac
     7261 cctacaataa ctacctatgg tatagcaaca aaaattaacg atacacatca taccagctat
     7321 ttcctcctaa tctcaccttc acacttacta aaatatagaa aaattcttgt catttttatc
     7381 ataacagtat aataggtctt aaaatccaat tgtctttcat gaatcgttaa taaataatga
     7441 agggaatgta atcgatacca tgcatattca ggaatttaat tattcaaata cagctcaacg
     7501 caggatttta ttgttatcgt tattcttttc aatagtgaca ctttcatcgt atgcaataat
     7561 gagtacgtta tttcttacct caatagtact ctatcaagcg gtatggaaat acatccttac
     7621 tctttccctt tcaggcattt taacgggtat catcattggt actatcatac ataaaaaaat
     7681 tagctttagc aggatgcttt taatactatt acaaatagta atatcatgtt tggcgatagc
     7741 tcctggtatt gcttcttact ttgataatgg cattgaaact atatatttag ccattgcaca
     7801 aaaattttct ctattgttct actgtttaat ctttcttctt tttacactgg agggtataaa
     7861 atccgcttac tttcttaaaa catctattgg cgattatatt gataataaaa atcctgccct
     7921 ttttgcaatc agtgtggcgt tgttttccat agttttttcc ttttatcttt cactgttact
     7981 atacagtcat atggaatatc tctggattta tttcgttttt cctttgctat cattaatttt
     8041 catcattttt ctcaacctac ctttttgccc acctaccatt atatcgcgca cagccataac
     8101 caaaccagat gatatttcaa ctcgcgatga tgttcttttt acctatagca atatttctat
     8161 catcagtata ttcacaactt tgatactgat aacaatttat tcacagcatg gaatttttat
     8221 agctacaaca atgtatacaa tcagcgctat tataggtggt tttttaattg gaatgtttct
     8281 atctgcattg ttaaaaaaat ataataccgt ctttatttat ggtgaaatgt tatatccatt
     8341 actgtttttg gcatttatta ttataagcca tacagcttct ccatcattat acagcagtct
     8401 tttagcagca gccccattca gcatatttgc aggaattaca tttggtacat cgttaaaaca
     8461 tatattaata aaaaacaatc actcaacacg gtataccatc attttatttt catttttcct
     8521 tatacccctg ccaataatta ttgcatttat ctggatagaa cttacgtact tctggttttt
     8581 tgctgtaacc tatatactgt caattattac catagtattt cccggtttat acctattgca
     8641 aaatttcaag cgctcggttc catccataat ctattgtatt gtcagtttat tattcatacc
     8701 tgctatcatc gttacccata catatttcaa acttccgtta agcgatagtc ttgttaatgc
     8761 atacagcaga ggatacgaag atgtggtttc atcaaatttc aactcttctt ttataaccaa
     8821 accgggagta gcttatatta atgattatgt agcctgccgt ataaccgatg caaccgcacg
     8881 cgatctgaaa agatccataa cattcatatc actgtttgca aacaatcaca acactattat
     8941 cattgatgga tatcacaaat tttttactaa cagccttttc catctcctga attcacccat
     9001 agtgctggat tatatctccg acagaatagt agattatcaa aaaccaccaa taagcgatgt
     9061 acattttgca accatacaca catctattat tcagggatta aaacaaaaaa aaggtttgat
     9121 agttgatata cccaatacct ttgatcatta tatgtatcct ttccgctttt ctgatgccta
     9181 catcgcaatc ataaaagaac atgttgatag ctactacttt acaataatag acataacaca
     9241 tattgcacca aactatctct atagtctgac aaatgcctta aaaaaatatt tcctccatac
     9301 agttgtgttt gtatccggca atcacctgac aataggagca accaataata gcaacggatt
     9361 tacccttgga gcatctgata ttcaaagttt agaaaacctg atacaaacaa ctgaggtggc
     9421 taatcttttt gttaatgctg agcatgcatt atcgtacatg gtatcaaata ccattgaatt
     9481 aatcatgcca cgcaatggcg tctttacaac atttacgcta cccattacca aaccagcaca
     9541 acaactattc atcaatccct ttgttgaaaa ggttattact accaatgacg ccgtagtaca
     9601 gatcattgat aaccagtact attatgctaa cactattgga ctgcgcatgc tatatgcaaa
     9661 acagatcaat cagctcatta aacaatccga atactatgga ttacagcaaa actatgaatc
     9721 agagatgcag acactgatgc aactacgcag gattgctgaa tatacccctc ccctgcggca
     9781 gtatataaca aacatcatta gctttaaaga aaaattgtat gaaaccattg cactggatta
     9841 cgaaaaacaa aaacaatggg acagggctat ttccctttac aatgcaatgt tagcggttaa
     9901 tcccaataac tttaccgcta attatcgcat tggtattatc aatgcaacca ttcagaattt
     9961 agaaaatgca tttaagtatt taaataatgc aatgcggatg gcccccaata accctgatgt
    10021 actgtatcag ttagggattc ttcttgtatc gcagggaaaa tttaatgagg cattgcagta
    10081 tcttacacag gcacatcaga tgaatatggc ttcggcatct ttgtttttta acattggttt
    10141 atgctatgaa tcactaaaaa acttagaaga agctaaacga tactatgaaa aagcaaaatt
    10201 tattgatccc aacgatgcta caatacaatc aagcctggaa agggtttcag aacaaaaagt
    10261 acagcaatca aatcaaaacg ctgatacaag aaccaaccag atagaagagg aaaaaggtga
    10321 aagtttccca ttaccaataa atcaatctgc ctatgatgtc cgcctcaaag aaaacgagct
    10381 gaaagatcag aacacatcca gcattcagca gtaacgttat agcatttttt actactattt
    10441 tttattatca tgaggttctg cgtaaacgga ttttcaatag gtattacctt atttggtgga
    10501 ttaggattgt ttttatttga catgaagata atgagcaaat ccctgcagtc agcagcaggt
    10561 gaacgtttaa aaagcatttt atggaaagtt agtaatagca gaatcaaggg agtattta
//
LOCUS       DAOSOX010000081        10582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6260_length_10582_cov_8.513442, whole genome shotgun sequence.
ACCESSION   DAOSOX010000081 DAOSOX010000000
VERSION     DAOSOX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6260_length_10582_cov_8.513442"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="PLX22_07130"
     CDS             complement(<1..1876)
                     /locus_tag="PLX22_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19710.1"
                     /translation="MKTKFLFFMYILWLAACTSCINSNNETIDISSNWKLFLINNSND
                     ISNLDFAQSDYDDSQWHSITLPGKLSHKKAKQHLWLRKTFVIPDSLQKYDIAFVLGKI
                     WDVEQTYCNGIRIGSAGREYPNFHSEWNNFRYYYIPSSLVRYNQPNTIAIRVFSNQNA
                     EYNGTPLVTTLHNAKIINFYRTLIAENIPLSTSVMTLLLGVIALYYYLRNRDVLIIQF
                     AIISLLWCFSAMHFYLPDYGIFDFNDQDKLYYALTAGISVSVYIFFETITRSYNKLLR
                     IFIIITALIMAILSLSATVNDPVTGWRFQVIGGLGLVVQILWGYLIFKGIKQNQKEAK
                     ALCIPYLFMMLCVGHDSLAVSGFIYTNFFWINLGYPGLILGIGAILSQRTAIVADELL
                     KSKEYIENKNSELTQILNKVNASIKELYDIAQNVRETTRLLTDNMHEQNSNIEQTSAA
                     LEEFSSTVDLIASNALHQDTILQKNKELLFEYIKGLGHITDAAKSAVKLSYKSQGQTS
                     VTKEHLDDALKGIEKIKNYSDNIRQIAETINDIAEKTNLLSLNASIEAARAGEHGRGF
                     AVVADEIGKLAERSIEQSKSIQTILSQILADIETQTLLMGTVVYSNEDVERSVYMVNH
                     A"
     gene            complement(2631..3329)
                     /locus_tag="PLX22_07135"
     CDS             complement(2631..3329)
                     /locus_tag="PLX22_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT19711.1"
                     /translation="MLFGIDPLYWMMMAPVLLISLWASFRVKSAFNKYSQIPSRSGYT
                     GGDVARIILQRNGLSHIPVEETTGFLSDHYDPIKKVVRLSPQVYNSNSLAAIGVAAHE
                     TGHALQHAKAYKPLVLRNAIAPTASIGSNLSWIIIFAGFIIGSLGLVKFGILLFSLVV
                     FFQLITLPVEFNASTRAKEILAGYGIVSSGELAGVSKVLSAAAMTYVAAAASAIMTLL
                     YFLIRAGFLGGSDD"
     gene            3575..5422
                     /locus_tag="PLX22_07140"
     CDS             3575..5422
                     /locus_tag="PLX22_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19712.1"
                     /translation="MKKILSALLLIVISCSSLPTSETKKDNIHYKETALELYSKALLL
                     KNEKKYTEAIALLAKASENTEQRDAIYYQMAECYFYQYNYEKAIEYADKAIAANDSFD
                     KPYILQYSVYMNLNQAEDATQVLEKLLIKRPQLYRIRFNLASVYYSQTKQNLKARYHF
                     LKIIEQSQYETIESYYKEQSYYFLGHIYYGFGEYKKAYQFFLKAYNENPDNYSILYIL
                     GLLNLEKGDIKQATVYLTQYLERYPNDIKAILHVGRIYYIYEDMKALPVLRKAINHKS
                     SEGTLAKMMYTEMLHNDDKALELAQKLRRMNSSLDMPYIALARIAMRNNDSSTAATNY
                     YTAGVLLYQKHHYDAAISMFVNALHSDAAIHDAYVYLGQIYEDTNQLQLAIYYYTMAY
                     KLKKNNDIMIRIGYIYTLLKDYTRAFEYFTMATSPDHTNPDVYFFKGLALLQKSDYAN
                     AGDMFRKALKIKRAEPYYFYLASALDKQKKIHDTISVLQEAYNNDMKSARICNYLGYL
                     YADNNMRLEESLVLINTALENEPTNGAYLDSLGWVYFKMGKYNDALPILIEASVELEM
                     EGYPDAVVYEHIGDTYKALQLHDKALEYWNKAYALDPRSDIQKKINAQH"
     gene            5453..6241
                     /locus_tag="PLX22_07145"
     CDS             5453..6241
                     /locus_tag="PLX22_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19713.1"
                     /translation="MNNRLLMTVIMVLCVLVYYGCKSTQISPPEKSDTDVTPLLEEII
                     KINTTMPHSINGAFRIEAFAKNSFKASAKLQYNVDEKKGKMTIVDSVFGSQISVVMRD
                     NDTLYIYQPIGNTVLVFNFTRFPVSSFLPFDIDVELINDFLTGRIPIIKNYNSAKAIK
                     QSNGESFILLENDQMHQTISMKENVPDKIMFVSKENSKRYEIYFIKPAVTNTLFSYKG
                     LRLVDVQSKTAFEITMQKYSINEPIPDDITDLKLPKNTKIIDYR"
     gene            complement(6232..>6498)
                     /locus_tag="PLX22_07150"
     CDS             complement(6232..>6498)
                     /locus_tag="PLX22_07150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT19714.1"
                     /translation="AEAMACGTPVIASDGGALREVVGDAGIVVPSRDEVSLASAIDNL
                     LGNAKLRQELSHKGIERVKTHFNWDSASAKMVALYKQVIATHQR"
     assembly_gap    6499..6598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6599..7153)
                     /locus_tag="PLX22_07155"
     CDS             complement(<6599..7153)
                     /locus_tag="PLX22_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT19715.1"
                     /translation="MRICLLCYRANPYCGGQGIYLKYVAEALSRQGHEVHAIVGPPYP
                     QPMDNVTVHYIDNNQYYIKKGFDIISHNSPFDIFHPINSYECIHSRFGAFPEISSFSY
                     RAFFAVRKLHKQFQFDIIHDNQCIGYGLLFMKALGIPVIATIHHPLTIDLENVLERGS
                     SFKNKIKGVMFYPVLMQQIVSKRLD"
     gene            complement(7150..7785)
                     /locus_tag="PLX22_07160"
     CDS             complement(7150..7785)
                     /locus_tag="PLX22_07160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT19716.1"
                     /translation="MDNYLKVSALKRFIFTIKTRLYWKLSYRKKFASIAGNPVIWGLW
                     NIDIYGPNIHLGKNVVFLAANGSKTALTTVQLAGHEGRIDIGNNVLIMNGVRISSASH
                     VRIGDDCMLANFCYLTDADWHDIHDRTNPVGKTAPIILDNGVWIGDSAIICKGVRIGQ
                     NSVVGAGAVVTRDVPSNVVVAGNPARIVKEIDPEKVITHSTLYKKVGTPRL"
     gene            complement(7933..8757)
                     /locus_tag="PLX22_07165"
     CDS             complement(7933..8757)
                     /locus_tag="PLX22_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19717.1"
                     /translation="MNQFFKTILHIIIGIICCCIVTNNSYSAEKLFSFPVGVTYDSIY
                     WWRGVELNGKGAGVIWPKVGCEVSGVQGYITIGINQDYYMTTEGGDNDVAKTYHEFDY
                     GLEYSFTQNTFSSTIGIKYCQYPFYDSAGATVDPSFFEAYGILSLSCVIIPVLELYYD
                     YYREESDEETPVNEDVYAKLSLFKEILHANSFIVKTGIWLGYYHNAYLDRDGFSDAGI
                     TLSTSYDYNSVVFFSAVNYARTLSEDFYIEYDADANGHARTLKNHLWVDIGISSKL"
     gene            9080..10561
                     /gene="lysS"
                     /locus_tag="PLX22_07170"
     CDS             9080..10561
                     /gene="lysS"
                     /locus_tag="PLX22_07170"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT19718.1"
                     /translation="MADDINDLVLQRIEKVKQLRTKGINPYPIRYKPTHTAQAIKEQF
                     GEEDKLLVKASGRIKSKRVMGKASFAHIEDASGLIQVYTRLDRLGEEKYELFKTLDLG
                     DIIGVAGHVFKTKQGEITIEVDDVVLLAKSIRPLPVVKEKDGKVFDEFADREQRYRQR
                     YVDLIVNPQVRQQFILRSNMITGIRNFLNQKGFIEVETPMMHAIPGGAAARPFITHHN
                     ALDMDLYLRIAPELYLKRLLVGGFDRVYELNRNFRNEGISTRHNPEFTMLELYQAYAD
                     YNDMMAITQELISTLAKELLQAIVIAYQGNTIDLTPPWQKLSYSEALKKYTGIDFTSI
                     SRDEAYTEALKRNVDVKKEMSLWKIAEEFFEEKVEENLIQPTFIIDYPKELSPLAKSR
                     EDNPEFVERFEVFIAGREMANAFSELNDPFDQRQRFEEQVKMREAGDDEAQMMDTDYI
                     TALEYGMPPAGGLGIGIDRLVMLFIDTASIKDTILFPLLKPEK"
BASE COUNT         3368 a         1924 c         2063 g         3127 t
ORIGIN      
        1 tagcatgatt taccatatat acagaacgct caacatcttc attggagtag acaactgttc
       61 ccatgagaag ggtctgggtt tcaatatcgg caagtatttg tgataaaatt gtttgaatac
      121 tttttgattg ctctattgat ctttcggcta atttccctat ttcatcggca acaacggcaa
      181 aacccctgcc atgttcaccg gcacgggctg cttctattga ggcattcaat gataacaggt
      241 ttgttttctc agctatgtca ttgatggttt cagctatctg gcgtatatta tcagagtagt
      301 ttttaatttt ttctatccct ttgagtgcat catctaaatg ctcttttgta acagaagttt
      361 gtccctgact tttatagctc agtttaacag ccgattttgc cgcatcagtg atatgcccaa
      421 gtcctttgat atattcaaaa agcaactcct tgtttttttg taagatagta tcctggtgta
      481 atgcattgga tgcaatcaag tcaacagttg atgaaaattc ctccagtgct gctgatgttt
      541 gttcaatatt gctattttgc tcgtgcatgt tgtcggttaa tagcctggtt gtttccctga
      601 cattttgagc aatatcatac aattctttga tggatgcatt gaccttattt aaaatctgag
      661 tcagttcact attcttgttt tctatgtatt cttttgattt gagaagttca tccgcaacta
      721 ttgcagttcg ttgtgataag atagcaccaa tcccaaggat gagtcctgga taacctaaat
      781 ttatccagaa aaagtttgtg tatataaaac cggaaacagc cagtgaatcg tgtcctacgc
      841 atagcatcat aaaaaggtag gggatgcaaa gagcttttgc ctctttttga ttttgcttta
      901 tacctttaaa aataagatag ccccatagta tttgtacaac caggcccaga ccaccaatta
      961 cctgaaagcg ccaccctgta accggatcat tgacagttgc tgataggctg aggattgcca
     1021 tgattaatgc tgttataatt ataaagatac ggagcaattt attgtagctc cgggttatcg
     1081 tttcaaaaaa gatatacacc gaaacagaaa tccctgcagt taatgcgtaa tataacttat
     1141 cctgatcatt gaagtcaaat atcccataat cgggaagata aaaatgcatg gcactaaaac
     1201 accataacag tgagataata gcaaattgta taatcaatac atccctgttt ctgagatagt
     1261 agtatagtgc tatgacaccc agcagtaaag tcataacact tgtggatagc ggaatatttt
     1321 ctgcaatgag tgtgcgataa aaattgataa tttttgcatt atgcaatgta gtgacgagtg
     1381 gtgtgccatt atattctgca ttttgattgc taaaaactcg tatggcaatg gtattgggtt
     1441 gattgtagcg tacaagtgag gaagggatat agtaatatct aaaattattc cattcggagt
     1501 ggaagttggg atactccctg cctgcagagc caatcctgat gccattacaa tacgtttgct
     1561 caacatccca tattttacct aaaacaaatg caatgtcata cttttgaagt gaatcaggga
     1621 taacaaatgt ttttctaagc cagaggtgct gttttgcttt tttgtgcgat agttttccgg
     1681 gaagggtaat gctgtgccat tgactgtcat cgtaatcaga ttgggcaaag tcaaggttgc
     1741 tgatatcgtt actattattt ataagaaata atttccagtt gctgcttata tctattgttt
     1801 cgttatttga gtttatgcag cttgtgcatg ctgctaacca taaaatgtac atgaaaaata
     1861 aaaatttagt cttcatataa aaatatcctg atgtatttat aactgtatat aatgaataat
     1921 ttagatatgt caacaagtaa aatactgctg taacaacagt tttacgcggg tggaagtttg
     1981 gtatgttcta ttgcaatggt cgtgcaacaa ttaataaaaa aactgctctg gtatatgata
     2041 ttgcctttat aggtgatacg gtattttgag agtgttggca tgcgcattaa tgccattaaa
     2101 attttgctgt taggtgttgc aataaaactt ccaataacat ggaactattc agtaccacta
     2161 aaaatactga ctgatcatga aattttctga tgcattataa ccatacatat atagcttacc
     2221 gaaataatgt tattaaattt atagtaaata ttttttatat aaaaattcta tctgggtggt
     2281 tacaataatc cggaaagagt gaaatttatt attgtaacaa cggaagtaat cagggatttt
     2341 ttacctgatc cagaaaaatt aatgaagaaa aaggaaccca ttctcttcag gctaagccat
     2401 aaaacgtctt tatcgggaca ttgcttgccc cataacgtta tatgagatga tacaatataa
     2461 aatgtgaaga tggcagtaaa aaaaaagaaa atgccagaag cagaaaagtg gaggaaggat
     2521 aggtatactg tatggccttg taattaaagt aaacctataa aaaaacagca tattggttca
     2581 acccgatata ctgtttttat tatagagcta tcaaaaatta attaaaataa tcaatcgtcg
     2641 ctgccaccta aaaaccctgc cctgattaaa aagtacaaca gtgtcataat agcagaagct
     2701 gcagcggcaa cataggtcat tgcggcagct gatagtactt tacttactcc tgcaagctca
     2761 cctgaactga ctataccata tcctgccaag atttcttttg cacgtgttga agcgttgaac
     2821 tcaacaggca atgtaataag ctgaaaaaat accacaaggc tgaaaagcag tatgccaaat
     2881 tttaccaggc caagtgaacc aataataaat cctgcgaaga tgataatcca tgataggttg
     2941 gagccgatag aagcagttgg agcgatagca tttcgtaaaa ctaaaggttt ataagcttta
     3001 gcatgttgga gggcatgccc tgtttcatga gcagcaacac caatggcagc taatgaattg
     3061 ctgttatata cctgtggtga cagacggacc actttcttta ttggatcata gtgatcggat
     3121 aaaaaacccg tggtttcttc aaccggtata tgtgataatc catttcgctg taatattata
     3181 cgtgctacat ctccaccagt atagccagaa cgtgacggaa tttgtgaata tttgttaaat
     3241 gcacttttca ctctgaatga agcccatagt gatataagaa gaacaggagc catcatcatc
     3301 cagtataatg gatctatgcc aaaaagcatg tactacctcc tcattgttat cattaaatta
     3361 tagtaattaa tgtatacaca gtcaatatac aaaatacata aaaaaatagt aactgcaaag
     3421 tatttttgta tcttaaaatt atattttgtt acaaaataat acataacgtc acaatatttg
     3481 caagttttat atttgacact aaccatcaac gcagtatatt gtacggtatc gtagtataca
     3541 gtttattgat ttatgtaagg caggaatcaa caccatgaaa aaaatccttt cagcattatt
     3601 gcttattgtt atatcatgtt catcactacc gacctccgaa acaaagaagg acaatatcca
     3661 ctataaagaa actgcgcttg agttatacag caaggcactg cttttaaaaa atgaaaaaaa
     3721 atatacagaa gctatcgccc tgcttgctaa agcttcggaa aatactgaac aacgcgacgc
     3781 aatctattac cagatggcgg aatgctattt ttaccagtat aattatgaaa aagcaattga
     3841 gtatgctgat aaagcaattg ctgccaatga tagctttgat aaaccatata tattacaata
     3901 ttcggtatat atgaatctga atcaggctga ggatgcaact caggtgctgg aaaagctatt
     3961 aataaaacgg ccacagctat accggatacg ttttaacctt gcaagtgttt attattcaca
     4021 aactaaacaa aatttaaaag cacgctacca ttttttaaag ataattgaac aaagtcaata
     4081 tgaaacaatt gaatcatatt acaaagaaca atcatattat tttttaggac atatctatta
     4141 tggttttggt gaatacaaaa aagcatatca gttttttttg aaggcatata atgaaaatcc
     4201 agacaattat tccatactgt atattctggg attattaaat ttagagaaag gtgatataaa
     4261 acaggctact gtgtacctaa cgcaatatct tgagcggtat cccaatgata taaaagcaat
     4321 actgcatgta ggacgcatct attatattta tgaagatatg aaggcactgc cagtactacg
     4381 taaggctatc aatcataaaa gctctgaggg tactcttgca aagatgatgt ataccgagat
     4441 gcttcataat gatgataaag ccctggaact ggcccaaaaa ctcagaagaa tgaacagttc
     4501 gcttgatatg ccttatattg ctcttgcccg tattgcaatg cgaaacaatg atagcagtac
     4561 tgccgctaca aattattaca ctgctggtgt gttgttatat caaaaacacc attatgatgc
     4621 agccatttcc atgtttgtca atgcgttgca tagcgatgct gcaatacatg atgcttacgt
     4681 gtatctggga caaatctatg aagataccaa tcagttgcaa ttagctattt attactatac
     4741 catggcgtat aaattgaaaa aaaataacga tatcatgata agaattggtt atatatatac
     4801 tttgcttaag gattacacca gagcatttga gtattttact atggctacct cccctgacca
     4861 taccaatcct gacgtttact ttttcaaagg acttgcctta ttacaaaagt ctgattatgc
     4921 caatgccggg gatatgtttc gtaaagcact aaaaattaag agggctgaac cgtattattt
     4981 ttatttagca tcggctctgg acaaacaaaa aaagatacat gataccatca gtgtgttaca
     5041 ggaggcatat aacaatgata tgaaaagtgc caggatatgc aactatctgg ggtatttgta
     5101 tgctgataat aatatgagat tagaagaatc gttagtactt attaatactg ctctggaaaa
     5161 tgaacctacc aatggggcat atttagattc attagggtgg gtgtacttca aaatgggaaa
     5221 atacaacgat gcattgccaa tactcataga agcatcagta gagctggaaa tggaagggta
     5281 tcctgatgca gtggtatacg agcatattgg tgatacatac aaagcgttgc agcttcatga
     5341 taaagcgctg gaatactgga ataaagccta tgcacttgac ccaaggtcgg atatacaaaa
     5401 aaagatcaat gctcagcatt gaaaaataat tacagggaaa ggaatgggaa cgatgaataa
     5461 cagattgtta atgacagtaa tcatggtact ctgtgtgctt gtatattatg gatgtaaatc
     5521 tacccagata agccccccgg agaaaagtga tactgatgtt acaccgcttt tagaagagat
     5581 tataaaaata aataccacga tgccccattc catcaatggt gctttcagaa tagaagcttt
     5641 tgcaaaaaac agctttaaag cctcagcaaa actgcaatac aatgttgatg aaaaaaaagg
     5701 aaagatgacc attgttgatt cggtgtttgg cagtcagatc agtgttgtaa tgcgggacaa
     5761 tgataccctg tatatatatc agcctattgg gaatactgtg ctggttttta attttacacg
     5821 tttccctgtt tcttcgtttt tgccttttga catcgatgtg gagctcatta atgatttttt
     5881 aactggtaga attccaatca ttaaaaatta taattctgcg aaagcaataa aacagagcaa
     5941 tggtgaaagt tttatacttc tggaaaatga ccagatgcat caaacgatat ccatgaaaga
     6001 aaatgtcccc gataaaatta tgtttgtttc aaaggaaaat agtaaacgct acgaaattta
     6061 ttttataaaa ccagctgtca ccaataccct tttttcatat aaagggttac ggcttgttga
     6121 tgtacagtca aaaactgctt tcgagattac catgcagaaa tattccatca atgagcccat
     6181 ccctgatgat attactgatc taaaattgcc caaaaataca aagattatcg attatcgctg
     6241 atgggtagca ataacctgtt tgtagagagc aaccattttt gcggaagcgc tatcccagtt
     6301 aaaatgtgtt tttactcgtt caatcccttt gtgcgatagt tcctgacgaa gctttgcgtt
     6361 gcccagaagg ttgtctattg cgctggctaa actaacctca tcgcgtgaag ggactactat
     6421 gccggcatca ccgacaacct ccctgagtgc gccaccgtca cttgcaataa ccggtgttcc
     6481 acatgccatt gcttctgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     6601 caagccgttt tgatacaatc tgctgcatga gaacaggata aaacatcaca ccttttatct
     6661 tgtttttaaa cgatgatccg cgttcaagaa cattttctaa atcaatggtt aacgggtgat
     6721 gtatggttgc tattaccggg atgcccaatg ccttcatgaa aaggagccca tagccaatac
     6781 actgattgtc atggatgata tcaaactgaa attgtttgtg aagcttgcgc actgcaaaga
     6841 aagcccggta gctgaagctg ctgatctcag ggaaagcacc aaatcgtgaa tgtatgcatt
     6901 cataactgtt gatgggatga aatatatcaa acgggctgtt gtgggagatg atatcaaatc
     6961 cttttttaat atagtattga ttattgtcga tgtaatggac agttacatta tccataggct
     7021 gaggataggg cgggcccaca atagcatgca cctcatgtcc ctggcgtgat agcgcctcgg
     7081 ctacatattt aaggtatatt ccctgccctc cgcagtaagg attagctcgg tagcataaaa
     7141 ggcatatcct cataggcgtg gcgttccaac ttttttatac agtgtactgt gcgttattac
     7201 cttttcagga tcaatctctt ttacaatacg ggcagggttg cctgccacca ccacattgga
     7261 aggcacatct ctggttacca ctgcaccagc ccccactact gaattttgtc caatcctcac
     7321 acccttacag attatagcag aatcgccaat ccagacgcca ttgtctaaaa tgatgggggc
     7381 tgttttgcca acaggattgg tacggtcatg aatatcgtgc cagtcagcat cagtaaggta
     7441 gcagaaattg gcaagcatgc aatcatcgcc tatcctgaca tgggaggcgc tgcttatacg
     7501 aacaccattc ataataagaa cattgttgcc tatatcgatg cgcccttcat gccctgctaa
     7561 ttgtacggtg gtaagggcgg ttttgctgcc gttagccgca aggaagacaa catttttccc
     7621 cagatgaatg tttggtccat atatatcaat attccataat ccccagatca caggattgcc
     7681 agcaatggat gcaaattttt tacggtacga tagtttccag tacaaacggg tctttatagt
     7741 aaaaataaat cgtttcaatg ctgaaacttt aagataattg tccatagtat gtcctttccg
     7801 ggttaagcta tgtgcaccct attcaaattt gattcatcgt caagagtttt ttttctatgg
     7861 ttcataaaca aaaggagtga tagttgtagc ctcactcctt taaaaagtga catagtgatt
     7921 ttggttatgc tattacagtt tgctgctgat gccaatgtca acccagaggt ggttttttaa
     7981 tgtacgggca tgtccattgg catctgcgtc gtactcaata taaaaatctt ctgaaagtgt
     8041 acgggcatag tttacagctg aaaaaaatac aacactatta taatcatagg atgtgctcaa
     8101 tgttattccc gcatcactga aaccatctct gtcaaggtag gcattatggt aatatccaag
     8161 ccatataccg gttttaacaa taaaactgtt tgcatgcagt atctctttaa acagtgataa
     8221 ttttgcatag acgtcttcat tgacaggtgt ttcttcatcg gactcttcac gatagtagtc
     8281 ataatatagt tccagaacgg gaataatgac gcaggaaaga ctcaatatcc cgtacgcttc
     8341 aaaaaaagat ggatcaaccg ttgcgccagc actatcataa aatgggtact ggcaatattt
     8401 tatcccgata gtgctgctga aggtattctg agtaaatgaa tattcaagtc cataatcaaa
     8461 ttcatgatat gtctttgcta catcgttatc gccaccttca gtggtcatat aataatcctg
     8521 atttatccct atagttatat atccctgtac tccgctaact tcacaaccaa cttttggcca
     8581 tataacgcct gcacctttac cgtttagctc aacaccacgc caccagtaga tactgtcata
     8641 cgttacccct acgggaaaag aaaataattt ttctgctgaa taactattat ttgtaacaat
     8701 acagcagcag attataccta taataatatg taaaatggtt ttgaaaaact ggttcatact
     8761 tacctccttt taaaaatata aagacgcatg ttatgtgcgt ctttatatta ttaattgtat
     8821 caagttcatg gtgtcaatgg cagagtatta ttcatctcat cattgtagaa aatgttacat
     8881 tgatgaaata tacctgtaat ctttatgtaa caaaaaaggg atgagcagga taaatactat
     8941 caaacaaatt ttcaattgac agcaattgct tctcttgtat catccagtaa catttgcagg
     9001 taactatcgc acaaaataga gaaaacaagc cgtttgtgct acattacagg catagtttat
     9061 ttttatgagg tatcccataa tggcagatga cataaacgac ttagtacttc agcgcattga
     9121 aaaagtaaag cagttgcgca ctaaaggaat caatccatac cctatccgat ataaaccaac
     9181 acatactgcg caggcaatca aggagcagtt tggcgaagag gacaaactcc ttgttaaggc
     9241 atccggaagg ataaagtcaa agcgcgtgat gggtaaggca agctttgccc acattgaaga
     9301 tgcaagcgga ctgattcagg tgtatacgcg gcttgacagg cttggagaag aaaaatatga
     9361 gctgtttaaa acattggatt taggtgatat tattggagtt gcagggcatg tatttaaaac
     9421 aaagcagggt gagattacca tagaggttga cgatgtagtg ctgcttgcca aaagcattcg
     9481 tccgttaccg gtggtaaagg aaaaagatgg taaggtgttt gatgagtttg ccgataggga
     9541 acagcgatac cgacagcgtt atgttgattt aattgtaaat cctcaggtac gacagcaatt
     9601 tatactgcgc agtaacatga ttaccggcat ccgcaatttc ctgaatcaaa aaggctttat
     9661 agaggttgaa acaccaatga tgcatgcaat accgggtggt gcggctgcac gtccttttat
     9721 aacgcatcac aatgcgcttg atatggattt atatttacgt attgctcctg aactgtattt
     9781 aaagcgactg cttgttggcg gatttgacag ggtgtatgag cttaaccgca atttcaggaa
     9841 tgaaggtata tcgacgcgcc acaatcctga atttaccatg ttagagctgt atcaggcgta
     9901 tgctgattac aacgatatga tggcaataac acaggagcta ataagcacat tggcaaagga
     9961 acttttgcag gcgatagtta ttgcgtatca gggcaatacc attgacctta cgccgccgtg
    10021 gcagaagcta agctatagtg aggcactgaa aaaatataca gggattgact ttacctccat
    10081 atcgcgcgat gaggcatata ccgaagcctt gaagcgtaat gttgatgtaa agaaagagat
    10141 gtcattgtgg aagatagccg aagagttctt tgaggaaaag gttgaggaaa atctcatcca
    10201 gccaaccttt atcatcgact atccaaagga actatcgcca cttgcaaaaa gcagggagga
    10261 taatccggag tttgttgagc gctttgaagt tttcatagca ggcagggaaa tggcaaatgc
    10321 attcagcgaa ctcaatgatc cgtttgatca gcggcagcgt tttgaggagc aggtaaagat
    10381 gcgtgaagcc ggcgatgatg aagcacagat gatggacacc gactatataa cagcgcttga
    10441 gtatggaatg ccaccggctg gcggacttgg cattgggatt gaccggcttg ttatgttgtt
    10501 tattgacacc gcttccataa aagatacgat attgtttccg ttgctgaagc cggaaaaata
    10561 aggagatata tgcaaacagt ta
//
LOCUS       DAOSOX010000082        10504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6327_length_10504_cov_8.894918, whole genome shotgun sequence.
ACCESSION   DAOSOX010000082 DAOSOX010000000
VERSION     DAOSOX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6327_length_10504_cov_8.894918"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLX22_07175"
     CDS             complement(<1..707)
                     /locus_tag="PLX22_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOT19719.1"
                     /translation="MGIVEWKKDENIAIITMNTVENRHNVMFAQAMLQALDEAEADRE
                     INGIVLTSSDAKNFSLGVDIEWLMPRMAEKDFKAIKDFMYGMNNVFKRLLLTPMPVIA
                     AINGHAFGNGAILSCACDFRFMRSDRGFFCFPEVDLGIPFLPGMIAFVKKALPYYKFN
                     EMKLTGRRVPAPELAEHHIIEKACANVDELMNESIAFAKSFKKKRGIFGEHKKRLHKH
                     IIEVIDKEDPEFIESLF"
     gene            complement(767..1135)
                     /locus_tag="PLX22_07180"
     CDS             complement(767..1135)
                     /locus_tag="PLX22_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19720.1"
                     /translation="MASPFILQNMYDSSLYICQERIWLQIIDLAFARGFNPVGTRLDY
                     YYELEQVWEEDMPYAEKIFTAIMIHVRCLNWDGCNFKDRENQIVSDDDCSELLYILND
                     TLPHDLKDFFKKGSFRICSE"
     gene            complement(1337..2368)
                     /locus_tag="PLX22_07185"
     CDS             complement(1337..2368)
                     /locus_tag="PLX22_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT19721.1"
                     /translation="MNKIVFACTRRSAGKTTIFTGLAKAMGKEVAYMKPFGDRLIYRK
                     KRLWDYDAALLTNLFGLEENPEDMTIGFEHAKLRYVYDEARIKEKLLEVAKSIAKNRE
                     VLFIESGKDLAYGVSVYLDPITVAKSLDAKLVLIVGGNDDVIIDDITLFKRYVDTSDI
                     NFLGVIINKIHDLDDFKSTYADKIEKMGVPILGMIPYSAELTYFSMDYLSEALFAKVI
                     AGESGLNKVVKNVFLGAMSVNEALRNPLFNKEGKLLITSGDRSDIIISALETNTVGIV
                     LTNNILPPSNIISKAAEKNIPLLLVSSNAYQVAGQIDKMEALLTKDNKEKIALAEKLV
                     KEHVNISAL"
     gene            complement(2381..4498)
                     /locus_tag="PLX22_07190"
     CDS             complement(2381..4498)
                     /locus_tag="PLX22_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT19722.1"
                     /translation="MKSKNSSIVTLFQPKSVAVVGASSQPGKIGYSITRNIIQGGFKG
                     RIYPINPKGGAILGVDCYPSISDIKDEIDVATICVPAKLTFDAVKECADKGVKHVQII
                     TSGFSEIGEVELEHKIVEYARSKGTRILGPNIFGVYSASGNYNSTFSATEILRGNVAI
                     LTQSGALGIAMIGKTAVENMGLSAIVSIGNKADIDEADCLEFVMRDQETKVILMYIEG
                     IKEGERFIEAVREATKLKPIVVLKSGRSKRGAMAAASHTGSLAGSDEIFDAIARQSGL
                     LRAENLQEAFNWCKFLAYSPRPKGKNTVIVTNGGGIGVMATDACEKYGVDLYDDQLVL
                     KEVFGPATPEFGSTKNPVDITGGAKAADYDLALSAPTRSEDMNATLALYCETATFDAE
                     NLAPMIRDTYGKHLASGKPVAYAVVGGRSVEDAIVQLRKMNVPVFPDVYEAVSCMGAL
                     YRYTTMLAERDDRVDDVDMDINTINKIIDGAIADGRTFLLANEGQEVMKASGVMIPGA
                     GIGKSIDECVRLAEQIGYPVVMKVVSRDILHKSDAGGVALDILNKQEVMDAYEAIMKN
                     CRAYKEDAVIDGIEVCEMVQKGVELIVGARRDPQLGPIVMCGLGGIYVEVMKDVAFRA
                     ATLNKKEARSMLSEIRSYPLLLGVRGEEQKDIDIVVDTIIKVATIIRKIPRITDIEIN
                     PVVVYEQGKGLKAVDVRIIISKS"
     gene            complement(4495..6393)
                     /locus_tag="PLX22_07195"
     CDS             complement(4495..6393)
                     /locus_tag="PLX22_07195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT19723.1"
                     /translation="MEQNTAKLEDYKRIYPEKFLPEEKIFSHINRGDRIFIGTACSEP
                     QYLVNALVEYVKSYPKAFFDTEVIHVWTLGVAPYADEKFKQNFRHNSFFIGMNTRNAV
                     NKGIADYTPIFLSRVPQLLRNGIIDIDVALVQTSYPDEHGYLSLGVSVDIVKTAVESA
                     RLVVVQVNKQMPRVHGDAFIHIKDVDFIVPHDEEILEFTTKDSDDISNRIGKYVSLII
                     QDGDTIQVGYGRMPNAILKHLIGKKNLGVHTELISDGIVELMKAGVVTNSEKTVDRGK
                     TIASFCMGTKPTYEYIHDNPSIEFKTIDYTNNPLIIAQQRRMTAINAALQIDLTGQAT
                     AESLGKSFFSGIGGQADFMRGAILAPGGKSILALRSTTEDEQTSRIVPFIPEGAGITL
                     NRGDVHYVVTEYGIAYIHGKNIRERAMDLIAIAHPKFRPWLIEEAKKDGLIYRDQAFI
                     PGKKGEYPEHLETFRTTKTGIKLFLRPAKISDEELLKDFFYSLSDQSLYRRFISTRKD
                     MPHERLQEMVVIDYTKELVILAVVEQNGKEVVVGVGQYGIQEYQHWAEVAFAVRDDYQ
                     YKGIGQVLLSYLTLIAKRNGLLGFTAEVLVENKPMLHLFEKMGFNIEKRSSAGVYELK
                     MEFRGDEQ"
     gene            6675..7376
                     /locus_tag="PLX22_07200"
     CDS             6675..7376
                     /locus_tag="PLX22_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19724.1"
                     /translation="MRLKLFILLFCIVAIVISYFYAPLVYYTYLKLYYQKICKIDEVA
                     FTNKVKDLYLSKQYDVLQQYLEHGAVLYPANKTIKKYNGLYLIDNGDVRGAFILIALD
                     AIPDDANTLEKIITVLDDNKSYQELLMIVHKKGIKTATIEFLYGKALCATGIYHQALS
                     HLKKAYTHGIVEADYYIGMCYHHLRDYNNALIWYKKALARKPGTVEYIKSIATVYQQK
                     GDYTSSTKYIMRLQH"
     gene            7580..8980
                     /locus_tag="PLX22_07205"
     CDS             7580..8980
                     /locus_tag="PLX22_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOT19725.1"
                     /translation="MKHELDYFFHPDGVAILGASGIAGSDGFYILRNVCAGYTGKVYP
                     VNPKYQELLGIPCYPDIESIPDRVECLIYFIPARFILDIIRRSAAKGIKAIIIESAGF
                     AEAGKEGAKLQKDAVELAHSLGIRLWGPNCMGYLDGHTRNVFSFVHSEKWKTMMYPGD
                     VTLIVQSGMLSAGFLMSVLERDAIGISKVCSIGNKCDVNEIDLLEYFVQDDTTGAIGC
                     YLESIVDGKKLLKIAQSTSKPIVILKSGRSEQGKKAALSHTASLAGNTMVVSGAFKQA
                     GIVQVVDLYELIDIVKAFSYIKNYRPEPDKGVAVMTFSGGAGIVTTDLLADYAIPIAQ
                     PQEATLQKVQKLYPSWMSASHPLDLWPAIEQNGSEKVYTSVAKALMEDERVDSIIIEC
                     FATQHSDPSFWGEIGNMVKQSKKPVLAWAVGEKIMVEQYKKHAEASGIPFFGEIGRCV
                     QIFKALRFHFNKRGSI"
     gene            complement(9033..10022)
                     /locus_tag="PLX22_07210"
     CDS             complement(9033..10022)
                     /locus_tag="PLX22_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19726.1"
                     /translation="MKLFTKVCLVLVLLFTSSTIFAEWVYVPMSINANKGDVVLSTSP
                     GFIMDLLAILGCYWSHSGMAVDNGFNIRHNTMYVSEIPIEYNKIWFIQTTPKRMNPTR
                     LSNGLPGILTEDIDTAYNATNNFAAAGGAVLKPTAANETAYRQYLNAAADVFNYLKAY
                     YRVNAYVNMYQLDYANYLITGRGNHCSGTCWYANYFSGKTMVVATIPANLVTQCANSL
                     YTSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGFDRATDTSDYWRSYI
                     GAVTATANAPDHLILSSYTNPSGHNVGAQTTSTSYYGQVEPLVITDGYYIEIP"
BASE COUNT         3158 a         2098 c         1952 g         3296 t
ORIGIN      
        1 aagaacaacg attcaataaa ttcggggtcc tctttatcaa tcacctcaat gatatgctta
       61 tgtaatcgtt ttttatgctc accaaatata ccacgttttt tcttgaagga tttagcaaat
      121 gcaattgatt cattcatcag ttcatcaaca ttggcacatg ccttttcaat aatgtgatgt
      181 tcagcaagct ctggggcggg aactcttctc cctgttaatt tcatctcatt aaatttataa
      241 tagggaaggg cttttttaac aaatgctatc atcccgggca aaaaggggat acctaaatca
      301 acctccggga aacaaaaaaa acctctatcc gaacgcataa acctgaaatc acatgcgcag
      361 gatagtatag cgccattacc aaatgcatgt ccattgatgg cagcaataac cggcatgggc
      421 gtaaggagca gacgcttaaa tacattgttc ataccataca tgaaatcttt aatagcttta
      481 aaatcttttt ctgccatcct tggcataagc cactcaatgt ctacgccaag gctaaaattt
      541 tttgcatcac tggaagtcaa tactatcccg ttaatttccc tgtcagcttc agcttcgtcc
      601 agtgcctgca acattgcctg agcaaacatg acgttatgtc tgttttcaac agtgttcata
      661 gtgataattg cgatattctc atcttttttc cattctacaa tgcccatata taccccctgt
      721 aaaatttatt taaaaaataa tagtaagtgc gcattatatg taacaattat tcactgcaaa
      781 ttctgaatga tcctttttta aaaaaatctt ttaaatcatg aggcaatgtg tcattcaata
      841 tatataaaag ttcgctgcaa tcgtcatcgc ttactatttg attttccctg tctttgaagt
      901 tgcatccatc ccagtttaag catcgaacgt gtatcattat tgcagtaaaa atcttttcgg
      961 catatggcat atcctcttcc catacctgct ctagttcata ataataatca agtcgggtcc
     1021 ctaccgggtt aaatccacgg gcaaacgcta aatcaataat ctgaagccat atacgttcct
     1081 gacatatata cagggaacta tcgtacatgt tttgtaaaat aaatggagag gccatagtac
     1141 accttcataa cactatatgc gtaacatcgt tatgtgctgt caaggctgtt ttatgtttta
     1201 ataattaaaa tttgagcgat agttaccggt aatggtagta tcattaaaaa ttagatgatt
     1261 gctgttcatc gatttattaa taaaaaaagg gctattgaag aatagccctt tttttataca
     1321 tgaaatatgt aacatattat agagcggaga tgtttacatg ttcttttacc agcttttcag
     1381 ccagtgcaat cttttccttg ttatcctttg taagaagagc ttccatcttg tcgatttgtc
     1441 ctgccacctg ataggcattg cttgaaacaa ggagaagtgg aatgttcttc tcagcggctt
     1501 ttgaaataat atttgatgga ggcaggatat tattggttaa gacaatccct accgtatttg
     1561 tttccagtgc cgatataata atatcacttc gatcgccgct tgtaatcaaa agtttacctt
     1621 ctttattaaa gagaggattt ctcagtgctt cgtttaccga catggcgccc agaaatacat
     1681 ttttaactac tttatttaat cctgattcgc cagcaataac ctttgcaaat agtgcttctg
     1741 acagatagtc catggaaaaa taggtaagtt ctgctgaata gggaatcatg cctaaaatgg
     1801 gcacacccat cttctcaatt ttatctgcat aggtgctttt gaaatcatca agatcgtgga
     1861 tcttgtttat gatcacacct aagaaattga tatcgctggt atcgacatat cgcttaaaga
     1921 gtgttatatc atcaataata acatcatcgt tgccaccaac aataagcact aattttgcat
     1981 caagtgattt tgccactgtt attgggtcta aatatactga aacaccatag gcaaggtctt
     2041 tcccactttc aataaagagc acttccctgt tctttgctat tgattttgca acttcaagaa
     2101 gcttttcttt gatacgggct tcatcataca catatcgcag tttagcatgt tcaaaaccaa
     2161 tcgtcatatc ttcgggattt tcttccaatc caaaaagatt ggtaagtaat gcagcatcat
     2221 aatcccatag ccgttttttc ctgtatataa gcctatcgcc aaagggtttc atatatgcaa
     2281 cttctttgcc cattgctttt gctaacccgg taaagattgt agtttttccg gcgcttcttc
     2341 gtgtacaagc aaatactatt ttattcataa tatacctcgc ttacgattta gaaataatta
     2401 tcctgacgtc tactgcttta agccctttgc cctgctcgta aacgacaaca ggattaatct
     2461 cgatatcagt gattcgcggt atcttcctga tgatagttgc aactttaatt attgtgtcaa
     2521 caacaatatc aatatctttt tgctcttcac cgcgaactcc aaggagcaat ggatatgatc
     2581 tgatttcaga tagcattgaa cgtgcttctt ttttgttaag ggttgcagct ctaaaggcaa
     2641 catccttcat aacttcaaca tagataccac ccaatccaca cataacgatt ggcccaagct
     2701 gtggatctct gcgtgcacca acaatgagtt caacaccttt ctgaaccatt tcgcatactt
     2761 caataccgtc tataacagca tcttccttgt atgccctaca gttcttcatt atagcttcat
     2821 atgcatccat tacttcctgt ttatttaata tgtctaatgc tacgccacca gcatcagatt
     2881 tatgtaaaat atcacgagaa acaaccttca taacaacagg ataccctatc tgctcagcca
     2941 gccgcacgca ttcatcaatt gatttaccaa tacctgcgcc tggtatcatg acaccagaag
     3001 ctttcattac ttcctgtcct tcattagcaa gcaggaatgt tctgccatcg gcaattgctc
     3061 catcaatgat tttattaatt gtgttaatat ccatatctac atcatccact ctatcatcgc
     3121 gttccgctaa catggttgta tatctatata atgcacccat acaggaaaca gcttcataaa
     3181 catccggaaa tacagggaca ttcatcttcc tgagctgtac aattgcatct tcaactgatc
     3241 taccacctac aacggcatat gcaacgggtt ttccacttgc cagatgcttg ccatatgtat
     3301 cacgaatcat tggcgctaag ttctcggcat caaatgttgc tgtttcacaa taaagggcta
     3361 acgttgcatt catatcttcg gaacgggtag gtgcactgag agctaaatca taatctgcag
     3421 ctttagcacc accggtaata tcaactggat tttttgtgga accaaattct ggtgttgcag
     3481 gcccaaaaac ttcttttagt acaagctgat catcatataa atcaacacca tatttttcgc
     3541 atgcatctgt tgccataaca ccaattccac cgccattggt aacaataacc gtatttttgc
     3601 cttttggacg aggcgaataa gccaaaaatt tgcaccagtt gaatgcttcc tgtaaatttt
     3661 ctgctctaag caatcctgat tgtcgtgcaa tagcatcaaa gatttcatct gaaccagcca
     3721 atgacccggt atgtgatgct gcagccattg caccacgttt tgaacgtcct gattttaaca
     3781 ctacgattgg ttttagcttt gttgcttcac gaacagcttc aataaaacgt tcgccttctt
     3841 taataccttc aatatacata agaatgactt ttgtttcctg atcgcgcata acaaattcca
     3901 gacaatcagc ttcatcaatg tcggctttgt tgcctattga tacaatagca gataatccca
     3961 tattttcaac agcagttttc ccaatcattg caattcccag tgcgccgctc tgggtcaata
     4021 tagcaacatt cccacgtaaa atctctgtag ctgaaaatgt tgagttgtaa ttgcccgatg
     4081 cagagtagac tccaaaaatg ttaggaccta aaatacgtgt tccttttgaa cgcgcatatt
     4141 ctacaatctt atgctcaagt tcaacctcac caatttctga aaatcccgag gtaataatct
     4201 gcacatgttt tacacccttg tctgcgcact ctttgactgc gtcaaaggtt aattttgcag
     4261 gaacacaaat agttgctaca tcaatttcat ccttaatatc cgaaatactt gggtaacaat
     4321 ctacacctaa tattgcaccc cctttgggat ttatagggta gatcctgcct ttaaaaccac
     4381 cctgtatgat attacgggtg atactgtacc caatcttgcc tggttgtgac gaagcaccaa
     4441 ctactgcgac acttttgggt tggaataaag taacaatact agaatttttt gatttcattg
     4501 ttcatctccc ctgaattcca tcttcaattc gtatactcct gcactactcc tcttttctat
     4561 gttaaacccc attttttcaa aaagatgcag cattggcttg ttttcaacaa gcacctctgc
     4621 agtgaatccc agcaaaccat ttcttttagc tatcagtgtt aaatatgata gcaatacctg
     4681 accaatacct ttatattgat aatcatcacg cacagcaaat gctacttctg cccagtgttg
     4741 atattcctgt ataccgtact gaccaacgcc gaccacaact tctttcccat tctgctcaac
     4801 aacagctaaa attaccagct ctttggtata atcaatgaca accatctcct gtaaacgctc
     4861 atgtggcata tcttttcgtg ttgaaataaa acgcctgtag aggctctggt cggatagtga
     4921 gtaaaaaaag tcctttaata gttcttcatc gctgatcttg gcaggtcgta aaaataactt
     4981 tatacctgtc tttgttgtac ggaatgtctc aagatgctca gggtattcac ccttcttgcc
     5041 cgggataaat gcctgatctc tgtaaataag accatctttc ttggcttctt ctataagcca
     5101 cggcctgaac tttggatgag ctattgcaat cagatccatt gcacgctccc taatgttttt
     5161 gccatggata taagctatcc catattctgt taccacgtaa tgaacatcac cacgattcag
     5221 ggtaatccct gctccttcag gtataaaagg cacaattcgt gaagtctgtt catcttctgt
     5281 ggttgaacgt aatgcaagga tgcttttgcc gccaggagca agtatagcac ctcgcataaa
     5341 gtcagcctgt ccaccgatac cgctaaaaaa acttttcccc agcgattcag cagtagcctg
     5401 accggttaaa tctatttgca gtgcagcatt aattgcagtc atcctgcgtt gctgcgcaat
     5461 gatgagcgga ttattggtgt aatcaattgt tttaaactca atagatggat tgtcatgaat
     5521 atattcgtag gtaggttttg tgcccatgca aaatgatgca atggttttgc cccggtcaac
     5581 tgtcttctca gaattggtaa caacgcctgc cttcatcaat tcgacaatgc catcagaaat
     5641 aagttcggta tgaacaccaa ggtttttttt gcctatcaga tgtttcagga tagcattagg
     5701 cattcttcca tatccaacct gaatggtgtc accatcttga atgataagtg aaacatattt
     5761 cccaatgcgg tttgaaatgt catctgaatc tttggttgtg aattctaata tttcttcatc
     5821 atggggtaca ataaaatcaa catcctttat atgtataaat gcatcgccat gaacgcgagg
     5881 catttgcttg ttaacctgga caacaacaag ccgtgcactt tctacggcag tttttacaat
     5941 atcaacacta acgcccaggc ttaaataacc atgttcgtcg gggtatgaag tctgaaccag
     6001 tgcaacgtct atgtcaatta ttccatttcg taacaactgg ggaacacgtg ataaaaatat
     6061 tggggtgtaa tcggctattc ccttatttac agcattgcgg gtgttcatgc caataaaaaa
     6121 agaattatgt ctgaaatttt gtttaaactt ttcatcggca tacggtgcca caccaagtgt
     6181 ccatacatga atcacctcag tgtcaaaaaa agctttagga tacgacttta catattcaac
     6241 aagagcattc actaaatatt gaggttcact gcatgctgta cctatgaaga ttctgtcacc
     6301 acggttaata tgactgaata tcttctcctc tggaagaaat ttctcagggt aaattctttt
     6361 ataatcttcc agttttgcag tattctgctc catacgcccc ctttcaatct gctatgtata
     6421 aaaaataaca gattgtatat aaaatatttt agccattaat tctgtcaatt caaattttta
     6481 tagtaaataa aatataataa tcagtatggt caatatttga ttattatatt aataatctat
     6541 ctatcatttt ttgacatgtc aaggaataat taataaaggc tttgaaatga agcaatagca
     6601 cggtataatg tttttatgtt gacgaaaatg aatgatatac agatattcgt caggttaatg
     6661 gctggtaatt aaatatgcgg ttgaaattgt ttatattgtt attttgtatt gtggcgatag
     6721 ttatctcata tttttatgcg ccgctggtat attacaccta tttgaaatta tattatcaga
     6781 agatttgcaa aatcgatgaa gtagctttta ccaataaagt aaaggattta tatttatcaa
     6841 aacaatacga tgttctgcaa caatatcttg agcatggcgc ggtactgtat ccagcaaata
     6901 aaacaataaa aaaatataac ggattgtacc tgattgataa tggagatgta cggggagctt
     6961 ttatacttat tgcattggat gcaattcctg atgatgcaaa tacattggaa aagattataa
     7021 cagtccttga tgataataaa tcgtatcaag aactactcat gatcgttcat aaaaagggaa
     7081 taaagactgc tactatagaa ttcctatatg ggaaagcttt gtgtgcaaca gggatatatc
     7141 atcaagcact atcgcactta aaaaaagcct acactcatgg tattgtagaa gctgattact
     7201 acattgggat gtgctaccat catctaaggg attataacaa tgctctgata tggtataaaa
     7261 aggcattagc gcgcaaacct ggtactgtag aatatattaa aagcattgca acagtatatc
     7321 agcagaaagg agattataca tcatcaacaa aatatatcat gaggttgcag cattaatgat
     7381 caatggggtg tttgcaaaag ctatgctgtg ctgtactcac tcaaatgaga cgaaactatg
     7441 tggcggatgt tatcctgagc catgatccat gaatatcttg attcaggata aagcccggaa
     7501 tgactggagc agcaaaaagg attacgggtt aagcctggaa taacattgag gaataattct
     7561 atttttggtg ggggaagcta tgaagcatga acttgattat ttttttcatc ctgacggggt
     7621 ggcaatttta ggtgccagtg ggattgctgg atcagatggt ttttatattc tgcgcaatgt
     7681 ttgtgctggt tatacaggca aagtgtatcc ggtaaaccct aaatatcagg agctactggg
     7741 tatcccgtgt tatcctgata ttgagtcaat tcctgacaga gttgaatgtt taatctattt
     7801 tataccggca cgctttattc tggatatcat cagaagaagt gcagcaaagg ggattaaggc
     7861 aattatcatt gaatcggcag gatttgcaga ggcaggcaaa gaaggagcca aattgcagaa
     7921 agatgcggta gaactggcgc atagccttgg catacggttg tgggggccca actgtatggg
     7981 atatttagat ggacataccc ggaatgtttt ttcatttgtc cattctgaaa agtggaaaac
     8041 aatgatgtat ccgggtgacg tgacgcttat agtgcaaagt ggcatgcttt cggcaggttt
     8101 tttaatgtcg gtactggaac gtgatgcaat aggtattagc aaggtatgtt ctattgggaa
     8161 taaatgtgat gtcaatgaaa tcgatctgtt agagtatttt gttcaggatg atacaacagg
     8221 agcgattggt tgctatctgg aatcaattgt tgatgggaaa aaactgttaa aaatagcaca
     8281 aagtaccagc aaaccaatcg ttatacttaa atcagggaga agtgaacagg gaaaaaaggc
     8341 tgctctgagc catacagcca gtctggcagg caatactatg gttgtatcag gggcttttaa
     8401 acaggctgga atagtgcagg ttgttgatct gtatgaatta attgatattg ttaaagcttt
     8461 ttcatatatc aagaactatc gcccggagcc agataaaggt gttgcagtga tgacattttc
     8521 aggcggcgca ggtattgtaa caactgattt attggctgat tatgctatac ccatagcaca
     8581 gcctcaagaa gcgacattgc aaaaagttca aaaactatat ccttcatgga tgagcgcttc
     8641 gcacccgctt gatctatggc ctgctattga acagaatggc tcggaaaaag tttatacgtc
     8701 tgtagcaaaa gcccttatgg aagatgaaag ggttgattct atcatcattg aatgctttgc
     8761 aacccagcat tcggatccat cgttctgggg tgagataggc aatatggtaa aacaatccaa
     8821 aaagccagtg cttgcatggg cagttggtga gaagataatg gttgaacaat ataaaaaaca
     8881 tgccgaagcc agcggtatac ctttttttgg tgagattgga cggtgtgtac agatattcaa
     8941 ggcgctgagg tttcatttca ataagcgagg gagcatataa aaaaatcccg ggattaaccc
     9001 gggatttttc tatgcattgt cagcgtgttc tactaaggga tttcaatata atagccatca
     9061 gtaatgacaa gaggttcaac ctgtccatag tatgatgttg atgttgtttg cgcacctacg
     9121 ttgtgccctg atggatttgt ataagatgaa agtatcagat gatcgggagc atttgctgtg
     9181 gctgttaccg cacctatata gcttctccag taatcggatg tatcggtagc cctatcgaag
     9241 ccgaaggtgt taacaatctg gttggctatt ttttcatcag cacctgtacc aaataagcct
     9301 tcaatatcga tgatgaaggc accaaaacct ccagcctcat cacgaaccat attttttact
     9361 gaggtataca gtgagttagc acactgtgtc accagattgg ctggtatggt tgcaacaacc
     9421 atggttttcc ctgaaaaata attggcatac cagcaggtac ccgagcagtg gttgccacgt
     9481 ccggtaatga gatagtttgc ataatcaagc tggtacatgt taacgtaagc attaacgcga
     9541 taatacgctt tgagataatt aaaaacatcc gctgcggcat taagatattg ccggtaggct
     9601 gtctcatttg cagctgttgg ctttaatacc gctccgcccg cagcagcaaa attattcgtt
     9661 gcgttatagg cagtatcaat atcttcggtt aggatgccag gcagtccatt actaagtcgt
     9721 gttggattca tccttttagg tgttgtttga atgaaccaaa tcttgttgta ttctattggg
     9781 atttcgctta catacatggt gttgtggcgg atgttaaatc cgttatcaac agccatgcca
     9841 ctgtggctcc aatagcagcc cagtattgcc aataaatcca taataaaacc agggcttgtt
     9901 gaaagaacta catcaccttt attggcatta atgctcatag gtacatatac ccattcagca
     9961 aaaatggtgg aactggtaaa taacaatacc agcacaagac atacttttgt aaaaagtttc
    10021 atataacccc caaacttata attaaattaa taaatgaacg ggatataaga gtcgatggca
    10081 aattgagtga ttgctcactc aacactattc attggataca ctatatgcac agtatcggaa
    10141 aaagtcaagc attatatacc atatggtcgg tttttatata ttttatagtt aagtaattat
    10201 tgagcagatt ggatgacata tattttcata tagtggtttc tatatttttt ttgctgtgac
    10261 taaaaaagat gttgaatcag atgcatatat tactcattct atactgtact attcaataac
    10321 gtggcattga tgagagggat gaaaatgaaa ccagccttac cctgataatc ctttttacag
    10381 aggacggttg ccaatcatgt caaagttagg tgcttgaagt atggtctata attaattcta
    10441 attttttggg agatacataa tgaaaaaaaa agttatagtg tattatagtt acattgttgc
    10501 tatc
//
LOCUS       DAOSOX010000083        10431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6387_length_10431_cov_8.214341, whole genome shotgun sequence.
ACCESSION   DAOSOX010000083 DAOSOX010000000
VERSION     DAOSOX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6387_length_10431_cov_8.214341"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLX22_07215"
     CDS             complement(<1..1025)
                     /locus_tag="PLX22_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOT19727.1"
                     /translation="MQILANILIIFSIAIAVIYICNRFKIPTIIGLLLTGLLVGPYGF
                     QFVKTPDEVELLSEIGIITLLFTIGLELSLEEITSLKRAVVAGVLQVLVTIVIVAVIM
                     LAWNSFSRSVFFGFIVSLSSTAIVLKILQEYALGDSPHGRISLAILIVQDIAIVPLML
                     FIPLLTGDVNHSITTEMLLLLLKGGVVIVLVLVLARYVIPKFLFEIAKTRSRELFIIS
                     VVTICLAITVATYSLGLSIELGAFLAGVIIAASEYSGQTFSSMIPFRDLFTSFFFISI
                     GMLLDPSIVMLNIITMSSLSLGVIAIKLFIITAIVIIVGYPLRTALLTGFALAQIGEF
                     SFILAQIG"
     gene            complement(1077..1544)
                     /locus_tag="PLX22_07220"
     CDS             complement(1077..1544)
                     /locus_tag="PLX22_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT19728.1"
                     /translation="MYEFREALLSDIDDVVTIRMKMLFELGKLETEDGTRKLRSLTRD
                     FFIRKFQTGEFKVFCAVTDETIIATTGIQFLDRPPLYENPHGIEAYIMNVYTEPDYRG
                     KGVASKLLDTVINYAKERKAGRILLHAIGKDKRIYEKKGFTSTTDEMQLILTY"
     gene            complement(1635..2048)
                     /locus_tag="PLX22_07225"
     CDS             complement(1635..2048)
                     /locus_tag="PLX22_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19729.1"
                     /translation="MIELSHNNYSSVLQSKLYNVTKHPYHFASFIAIWYYVELLYMMS
                     IAVFMYPPMLIAIVAIVTGIALSFHIIKLYSGNDFSCSLQLLLMDIHIAFSIGTIATL
                     LLSNVGWYILLFTLIRAIVALCEVVCVYAMTNNKT"
     gene            2127..3083
                     /locus_tag="PLX22_07230"
     CDS             2127..3083
                     /locus_tag="PLX22_07230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT19730.1"
                     /translation="MDDKKTITLTVPDEYSNTRIDVFIFNALEGKLSRSFIQKLLKGG
                     NITVNGNSVKQNYKVKHQQEITIAIPKPEKTSLQPQDIPLTILFEDNDIAVIYKPAGM
                     VVHPGAGNVEHTLVNALLYHFKGLSSIGGVERPGIVHRLDKDTEGLMIIARNDTAHTI
                     ASKAFQQRTVIKKYEAIVIGKPNEVSGLINKAIDRHPKYGHKMAVRDDGKEALTQYTC
                     KEIWHTANGVFSRLELQIHTGRTHQIRVHLASIGLPIVGDQLYSKKWEKYRVPYMLLA
                     ATHLQFEHPITKQPLQFYVDLPIHIKDFITRLNTLAIEHIIP"
     gene            3090..3518
                     /gene="tsaE"
                     /locus_tag="PLX22_07235"
     CDS             3090..3518
                     /gene="tsaE"
                     /locus_tag="PLX22_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT19731.1"
                     /translation="MKQVHYTTSSQEETFAVGYELGKKAKKGDIFCLIGDLGTGKTIL
                     AKGIAKGLGINEEITSPTFTLLEVYEAAIPLYHFDLYRISDDSELENLFFEEYWYGDG
                     VSVIEWAERAMKRLSNDIFIIRLSYTGKNRRKITIEHPDP"
     gene            3499..4191
                     /gene="tsaB"
                     /locus_tag="PLX22_07240"
     CDS             3499..4191
                     /gene="tsaB"
                     /locus_tag="PLX22_07240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT19732.1"
                     /translation="MNILILDTATSIEIVAIRYEGTIADKSSATQNSHSVTLFSSIDD
                     ACKELGITIHDINCIAVGIGPGSFTGIRIAVTTARMLAQILEVPLVGIHTQMLYACSI
                     VTDNVDCIVAFDAKKSRVFAARYRIQQGKLPQVVIPPGDYPVRHLCEIDTSKHIIAIG
                     DGALKYNNEMTALIKNITFLPGFLPQPEAMVAMIENLYTDNPQKYADYHQVVPFYSRK
                     SDAEIMRELKNK"
     gene            complement(4206..5384)
                     /locus_tag="PLX22_07245"
     CDS             complement(4206..5384)
                     /locus_tag="PLX22_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HOT19733.1"
                     /translation="MKEYKQRKSRNFENVATLDDVMELLKDEQFQKKLMIKFTLDKTS
                     IEINEFIDNRTIMLVTDPDYVPVQNKIILYGLADRYLEIECDVIEVAGPGYFKCKIVS
                     ARKAAHGRRDLRFKLNIEKVVATNFRVSKHTIDIRNYSIPTGIKVIVEQFENQISKNA
                     DIVKVDILDDRDPLLAHIRKTKSTFYIEDLNDPSTFAPVNDAFININDVFQDQTAQFV
                     KKLTDSGYKSIIISPVIYIENDERLIPFAFIQYISKENTFSMDKVLEVQDLAYKLVDR
                     IRDANTLMIAVHQEIVDISRGGAKLRITDSNLKKYILKSKGFIFDIVFKLQAPITIYG
                     DIRYTYLDNDDNVIVGVDFAGNSSRKDEMKRFYSIIQPMENEYRNRLIKEMRQKKKDQ
                     "
     gene            complement(5540..5740)
                     /locus_tag="PLX22_07250"
     CDS             complement(5540..5740)
                     /locus_tag="PLX22_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19734.1"
                     /translation="MEQKKSLKQLQAEEVRKKDKVKKLNLELNALKKDLQALAKSILS
                     AKKLEAEKKKAAPKSKKPAKKK"
     gene            6110..7414
                     /gene="der"
                     /locus_tag="PLX22_07255"
     CDS             6110..7414
                     /gene="der"
                     /locus_tag="PLX22_07255"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT19735.1"
                     /translation="MKDIPVVSIIGRQNVGKSTLFNAIIKQRKAIVDEQPGLTRDVVS
                     YFVNYNEATFIINDTPGLDLNANSTLSEEIKQMALQHLHSTDVFVLLLEKPDIASYDF
                     ELVELIRKFNKPYIVAINKIDTNNDMECLSNFYELAQEFLPISALNRRNIDLLLHTIV
                     ESLPHKRKKIIEIDCKIALVGRPNSGKSTLLNAFLGYQRAVVSDIPGTTRDSIDEIFR
                     FQGKNIMIIDTAGIRRQSRIDNSIEYYAIVRAREAIQKSDVVIHLLDGTSGITETDKK
                     IADIIMEAHKPTILAINKWDSIPKDHTTFESFKDRMIFKYYRAADFPIISISALHKLR
                     IHKLLLTALDVNNRAHHKTETSAINVIIENIQKKHAMPLLGGDLKIYYATQTNISPPV
                     IRLFVNKPELFRKDVYRFLEKTLQDELNWQGIPVVFEVSGRK"
     gene            complement(7416..8012)
                     /gene="recR"
                     /locus_tag="PLX22_07260"
     CDS             complement(7416..8012)
                     /gene="recR"
                     /locus_tag="PLX22_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT19736.1"
                     /translation="MNLPSKYLEQCINELSKLPGIGVKSASRIAFYLLKVPKPNVDTL
                     LTTIAQLKNNMQYCKQCGGISDADICTICSDNERDRTLLCVVEEPEDMLSIEKSGAFN
                     GLYHVLGGVISPLDGVGPEELSINKLVDRCKQGVNEVIIATNPTIEGDATALYIASLL
                     KPMNIKVMRIAHGLPVGSDLEFVDAPTLAKSITGRREM"
     gene            complement(8014..8343)
                     /locus_tag="PLX22_07265"
     CDS             complement(8014..8343)
                     /locus_tag="PLX22_07265"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT19737.1"
                     /translation="MNFKDVGQLMKAQSELKKIQKRLKKNEITASSRDEMVKATVNGD
                     YELVSIAIDEEKCKGSSIKTLERNITEAVNKAIADAKEFSSQEMMKISGEMDIFGMAN
                     ELKKFIK"
     gene            complement(8358..8708)
                     /locus_tag="PLX22_07270"
     CDS             complement(8358..8708)
                     /locus_tag="PLX22_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19738.1"
                     /translation="MHAWTQMLHDWRNARQYLFFKLTNVRTAYVNGQLVLSVMGDSSN
                     GFYKQLLSKEEIKLIEQELYNRSKRNVSIKIKSGEDNTPPPDAVMLPHAEDPEKPHPL
                     AEQIKTMFDGEIIK"
     gene            complement(8773..>9002)
                     /locus_tag="PLX22_07275"
     CDS             complement(8773..>9002)
                     /locus_tag="PLX22_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19739.1"
                     /translation="HDEQLSLFYTMLLDCQNQLRFTGNERAVVEMFLIDIMKMLQRPT
                     LATILQQLDTASPEPVQKKKYRESCTEESYN"
     assembly_gap    9003..9102
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9103..9671)
                     /gene="dnaX"
                     /locus_tag="PLX22_07280"
     CDS             complement(<9103..9671)
                     /gene="dnaX"
                     /locus_tag="PLX22_07280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT19740.1"
                     /translation="MSYQVIARKWRPQTYSDVVAQEHVSRTIANSISKGRISHAYLFA
                     GPRGVGKTTMARIVAKSLNCINGPTDNPCGVCEFCTEIKNGNSFDVIEIDGASNTGVE
                     NIRELRENIHSAPLKSRYKVYIIDEVHMLSKSAFNALLKTLEEPPAHVVFIFATTEIQ
                     QIPETILSRCQKFIFKRIPVASIVAQLLK"
     gene            complement(9697..9782)
                     /locus_tag="PLX22_07285"
     tRNA            complement(9697..9782)
                     /locus_tag="PLX22_07285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9746..9748),aa:Ser,seq:gga)
BASE COUNT         3290 a         2024 c         1721 g         3296 t
ORIGIN      
        1 ccaatttgag ccaatataaa tgaaaattca cctatttgtg ctaatgcaaa gcccgtcaat
       61 aacgcagttc gcaaaggata tccaacaata ataacaattg ctgtaattat aaacaatttg
      121 atagcaataa cacccagtga taatgatgac atggtgataa tattcaacat aacaatggaa
      181 ggatcaagga gcatcccaat agatatgaag aaaaagctgg taaataaatc tctgaatggt
      241 atcattgaac tgaatgtttg cccgctatat tctgatgctg caatgatgac acctgcaagg
      301 aatgcgccta actcaatgga taaccccagt gaatacgttg ccacagttat tgccagacaa
      361 atagtaacaa cacttataat aaaaagttct ctgctacgcg tttttgctat ttcaaataaa
      421 aattttggta tgacatatcg tgccagtaca agtaccagca caatgacaac gccgcctttt
      481 aaaagcaata aaagcatttc agtagtaatt gaatggttca catcaccagt caataaagga
      541 ataaataaca tcaatggaac aattgcaata tcctgaacta ttaatatcgc cagtgatatt
      601 cttccatgag gactatcgcc aagagcatat tcctgtaaaa tttttaaaac aattgctgta
      661 ctgctcagtg aaactataaa tccaaaaaaa acagatcgtg aaaaactatt ccatgcaagc
      721 attatcacgg caacaatgac tatcgtcaca agcacctgta aaacaccagc aactactgct
      781 cgttttaagg atgttatttc ctcaagcgat aattcaagac caatggtaaa aagtaaggta
      841 atgataccta tctcggataa cagctcaacc tcatcaggtg ttttgacaaa ctggaaacca
      901 taaggaccta ccagcaaacc tgtcagcaac aagccaataa ttgttgggat tttaaaacgg
      961 ttacatatat atataacagc tattgcaatt gagaaaataa ttaatatgtt tgcaagaatt
     1021 tgcataggtt atgcaactca ggattgaatt aaagtattgt tgaacaatcg aaataattaa
     1081 tatgttaaaa tcaactgcat ctcatcggta gttgatgtaa agcctttttt ttcataaata
     1141 cgtttatctt tgccaattgc atgaagcaat atccggcctg cttttcgttc ttttgcataa
     1201 ttgataactg tatccagcaa tttagatgct acacccttac ctctataatc cggttcagta
     1261 tacacattca taatatacgc ttcaatacca tgtggatttt catataatgg agggcgatct
     1321 aaaaactgaa tacccgttgt agcaataatt gtttcatctg tcacagcaca aaaaacctta
     1381 aattctcctg tctgaaattt gcgaataaaa aaatccctgg taagtgagcg aagcttccgc
     1441 gttccatctt cagtttccaa tttacccaat tcaaaaagca tcttcatcct tatagtaaca
     1501 acatcgtcaa tatcacttaa cagtgcttca cgaaattcat acatatatac cccctcatga
     1561 acaactaaac cttttagcaa aatccattaa tgcgtattgt atttgtttcg tcaaataata
     1621 aagttgtatc agtatcatgt tttattattg gtcatagcat aaacacaaac cacttcgcat
     1681 aaggctacaa ttgcgcgtat taaggtaaaa agcagtatat accatcctac gtttgaaaga
     1741 agtaaggtag ctatcgttcc tattgaaaat gcgatatgaa tgtccatcaa gagcaactgc
     1801 aaagagcaac taaaatcatt gccgctataa agctttataa tatgaaagct aagcgctata
     1861 ccagtaacta tcgccacaat tgcaattaac attggcggat acataaaaac tgctatactc
     1921 atcatataca aaagctcaac ataataccag atagcaataa aacttgcaaa atgataggga
     1981 tgttttgtta cattgtacag cttgctttgc aatacgctac tataattgtt gtgcgatagt
     2041 tctatcacta ttgtcctcag aaaaaaatga tttgaccgtt tgtttaacat tattattaca
     2101 atgctttagt atttcaaggc ttttttatgg atgataaaaa aactattacg cttaccgtcc
     2161 ccgatgaata tagcaataca cgtattgacg tgttcatatt caacgcgctt gaggggaaac
     2221 tttcgcgcag ttttatacaa aagctcttaa aaggtggcaa cattaccgtc aatggaaatt
     2281 ctgtaaaaca aaattataaa gttaaacacc aacaggaaat taccatagct atacccaaac
     2341 ctgaaaaaac atcattacaa cctcaggaca tccccctgac cattctattt gaagataacg
     2401 atattgcggt catttacaaa ccagcaggca tggtggtaca tcccggagct ggcaatgttg
     2461 agcatacgct ggtgaatgct cttctttacc atttcaaagg attatcatcc attggcggtg
     2521 ttgaacggcc tggcattgtt caccgccttg ataaagatac tgagggtttg atgattattg
     2581 ccaggaatga tactgcccat actattgcat caaaagcatt ccagcaacga acggttataa
     2641 aaaaatatga ggcgatagtt atcggtaaac ccaatgaagt atcggggctt atcaataaag
     2701 caattgatcg ccatccaaaa tatggacata aaatggctgt gcgtgacgat ggcaaagaag
     2761 cgctgacaca gtatacctgt aaggagatat ggcataccgc caatggtgtt ttttcgcgat
     2821 tagaattgca aatccatact ggacgcaccc atcagatacg ggtacattta gcttcaattg
     2881 gacttcccat agttggtgac cagctttatt ctaaaaagtg ggaaaaatac agggtgccct
     2941 atatgctgct tgctgctacc cacctgcagt ttgaacaccc cattaccaaa caacccttgc
     3001 aattttatgt tgacttgccc atacatatca aagattttat taccaggctc aatacacttg
     3061 cgatagagca tataataccg taaaatagta tgaaacaggt acactatact accagctcac
     3121 aggaagaaac atttgctgtt ggctatgaat taggcaaaaa agcaaaaaaa ggtgatattt
     3181 tttgcttaat tggcgatctg ggaacgggaa aaactatact tgcaaaggga atagcgaaag
     3241 ggcttggcat aaatgaagaa atcaccagtc caacatttac cctgcttgaa gtgtatgaag
     3301 ctgcaatacc gttgtatcat tttgaccttt atagaatatc tgatgattca gagctggaaa
     3361 atttattttt tgaagaatac tggtacggtg atggtgtatc cgtcattgaa tgggcagaac
     3421 gagctatgaa aagactctcc aacgatatat tcatcatcag gctttcatat actggcaaaa
     3481 acaggaggaa gattaccatt gaacatcctg atccttgata cagcaaccag cattgagatt
     3541 gtagctatac gttatgaagg cacaatagct gataaaagca gtgcaacgca aaattcccat
     3601 tccgtaacat tgttttcatc catagatgat gcatgcaaag agcttggcat aactattcat
     3661 gacattaatt gcattgcggt agggattgga ccgggttcat tcaccggcat ccgcattgcc
     3721 gttactactg cccgcatgct tgcacaaatt ctggaagtac cgctggttgg tattcataca
     3781 cagatgttgt atgcctgttc gatagttact gataatgtgg attgtattgt agcatttgat
     3841 gcaaagaaat cacgggtgtt tgcagctcgc taccgtatac aacagggcaa attgccccag
     3901 gtggtaatac cccctggcga ctatcctgta cgccatcttt gcgagattga taccagcaaa
     3961 cacatcattg ccattggtga tggtgcgctg aaatataaca atgaaatgac agcgttaata
     4021 aaaaatatta cttttttacc cggtttttta cctcaacctg aagctatggt tgccatgata
     4081 gaaaatcttt atactgacaa tcctcaaaaa tatgctgatt accatcaggt tgtgccattt
     4141 tattcacgaa aatccgatgc agaaataatg agggaattaa aaaataagta ggctttatac
     4201 tattgttact gatctttttt cttttgacgc atttctttaa tgagacggtt tctgtattca
     4261 ttctccatgg gctgaattat tgaataaaac cgcttcatct cgtccttccg ggaggaattt
     4321 ccggcaaaat ccacgccgac aatgacatta tcatcattat caaggtaggt atagcggata
     4381 tctccatata ttgtgatggg tgcctgcagt ttaaatacaa tgtcaaatat aaaccctttt
     4441 gatttcagta tatacttctt caagttacta tcggtaatcc ttaattttgc cccaccgcgg
     4501 ctgatatcta ctatctcctg atgtactgca atcatcaatg tgtttgcatc tctaatccta
     4561 tcaacaagct tataggctaa atcctgaacc tcaagtacct tatccataga aaatgtattt
     4621 tcttttgata tatattgtat aaatgcaaat ggaatgagac gttcatcgtt ctctatgtaa
     4681 ataaccggag atataataat tgatttataa ccactgtcag ttaatttttt tacaaattgt
     4741 gctgtttgat cctgaaaaac atcatttata ttaataaacg catcattgac tggagcaaag
     4801 gtagatggat cattaagatc ttctatataa aaggttgatt ttgtttttct gatatgtgca
     4861 agcaatgggt ccctgtcatc cagtatatca acctttacta tatcagcatt ttttgaaatt
     4921 tgattttcaa attgttcaac gataactttt atacctgtag gaatactata attacgaata
     4981 tcaatagtat gtttggaaac cctgaaattg gtggcaacaa ccttctcaat atttaatttg
     5041 aagcgaagat cacgtctacc atgcgctgcc ttgcgtgcac tcacaatttt acatttaaaa
     5101 tatcccggtc ctgctacctc tataacatca cattcaatct ctaaatacct atcggcaagc
     5161 ccatacaaaa taattttatt ttgtacagga acataatctg gatcagtaac aagcattatt
     5221 gttctattat ctataaattc attaatttca attgatgttt tatcaagggt aaatttaatc
     5281 attaattttt tctggaattg ttcatctttc aacaactcca taacatcatc taaggttgct
     5341 acattctcaa aatttcgaga ttttctttgt ttatattcct tcaaattacc ctcaatacac
     5401 agaacaattg gtttatgcta tattttttat cgaaaatttt atatatttac ttcaccataa
     5461 aacattaaaa ttttttataa acaaagaggt ggataaccac ctctttaata atattaatga
     5521 ttaggtaata tcaaaaaact tatttcttct tagcaggctt ctttgatttt ggtgcagctt
     5581 ttttcttttc tgcttccagt ttttttgcag ataaaatact ttttgctaat gcttgtaagt
     5641 cttttttcaa agcattaagc tcaagattta actttttaac cttgtctttc tttcgtactt
     5701 cttcagcctg aagctgtttt aatgactttt tttgttccat cgattacacc acctcgattt
     5761 aaaatttttt acatgcatgg cgcatgatta aaaagtaaac aattaagcct gatcatgatc
     5821 atataacata tatattttaa tattgcaaca aaaaaatgat tttgatatat gaaaatatta
     5881 attttttttt aacatcattt tttataaata ttacttttat tttattaatg tatcaatcga
     5941 tctatattca taaaaacatt agaaattaat ttccatatca ataaatatct attgacacca
     6001 tacatatatt catcagtagt agaaacaatc tgtagtactg tcgctactat caataattat
     6061 gcttgaaatc attatgattg gcaacaatca ttatagtaag agggatactg tgaaggatat
     6121 acctgtcgtt tcaataattg ggcggcaaaa tgttgggaaa tcaacccttt ttaatgcaat
     6181 tatcaaacaa cgcaaggcaa ttgttgatga acagcccgga cttacccgtg atgttgtttc
     6241 atattttgtg aactataatg aagcaacatt catcatcaat gacaccccgg ggcttgatct
     6301 aaatgccaat agtacactat ccgaggaaat taagcagatg gcattgcaac atctccattc
     6361 tacggatgta tttgtgttat tactggaaaa accggatatc gcttcttatg attttgaact
     6421 ggtagaactc atccgtaaat ttaataaacc atatattgtt gccattaata agatagatac
     6481 caataacgat atggaatgtc tctctaattt ttacgaattg gctcaggaat ttctgcccat
     6541 ttctgcatta aaccgccgca acattgatct actccttcac actatagttg aatctctgcc
     6601 gcataagagg aaaaaaatca ttgaaattga ttgtaaaata gctcttgtag gacgtccaaa
     6661 ctcaggaaaa tcaacattat taaatgcatt tttaggatat cagcgtgcag tggtatcaga
     6721 cattcccggt accacacgtg atagcattga cgaaattttc agatttcaag gtaaaaacat
     6781 catgattatt gatacagcgg ggatacgacg acaaagccgt attgataatt caatagaata
     6841 ctacgccatt gtccgtgcca gagaagctat tcagaaaagt gatgtagtaa tccatctttt
     6901 agatggaaca agtggtataa ccgaaactga taaaaaaata gctgatatta tcatggaagc
     6961 acacaaacca acaatacttg caattaacaa atgggatagt ataccaaaag atcatacaac
     7021 ctttgaatca ttcaaggata gaatgatttt caaatactat cgcgcagctg atttccctat
     7081 tatttccatt tcagcattgc ataagcttag aatacataaa ttgcttttaa cagctttaga
     7141 tgtcaacaac agagcacacc ataaaactga aacgtctgca ataaatgtaa ttatagaaaa
     7201 tattcagaaa aagcatgcca tgccactcct tggtggagat cttaagatat actatgcaac
     7261 gcaaaccaat atttctccac cggttatacg gctttttgtc aacaaaccag aactcttccg
     7321 aaaagatgta tatagatttt tagaaaaaac attgcaggat gaacttaact ggcagggaat
     7381 tcctgttgtt tttgaggtaa gcggtagaaa ataggttaca tctcccttcg tccggtgatg
     7441 gattttgcaa gtgtgggtgc gtcaacaaat tctaaatcag agccaaccgg caagccatgc
     7501 gcaatacgca taaccttgat gttcatcggt ttcagaaggg atgcaatata cagcgctgtt
     7561 gcatctcctt ctattgttgg attggtggca ataataacct cgttcacacc ctgtttgcat
     7621 ctgtcaacaa gcttgttgat tgaaagctct tccggcccaa caccgtcaag aggcgaaata
     7681 actccaccca gtacatggta caaaccatta aaggcacccg atttttcaat actcagcata
     7741 tcctcaggtt cttcaaccac acataaaagt gttctatcac gctcattgtc actgcatatg
     7801 gtgcagatat cagcatcaga tatgcctcca cactgtttac agtattgcat attatttttt
     7861 aactgggcga tagttgtcag caatgtatca acattaggtt taggaacttt cagtaaatag
     7921 aatgcaatgc gcgacgcact cttcacgcca atacccggta attttgacag ctcattgata
     7981 cattgttcta aatattttga aggcagattc atgctacttt ataaactttt taagttcatt
     8041 tgccatacca aaaatatcca tctcgcctga tatcttcatc atctcttgag atgaaaattc
     8101 ttttgcatca gcaattgctt tattcaccgc ttcagtgata tttcgttcca atgttttaat
     8161 actgctgcct ttacattttt cttcatcaat agcaatggaa accagttcat agtcaccatt
     8221 gaccgttgcc ttcaccattt catcgcgtga tgaagcggta atttcgtttt ttttaagcct
     8281 tttctgaatt ttttttaact cgctctgtgc tttcatcaat tgtccaacat ctttaaaatt
     8341 catagtgttt acctctacta ttttataatc tcaccatcaa acatggtttt aatctgttct
     8401 gctaatggat gcggcttttc aggatcttca gcatgtggca gcatcactgc gtcaggaggc
     8461 ggcgtattgt cttctccaga tttgattttt attgatacgt ttcgtttact gcggttataa
     8521 agctcctgtt caataagttt tatttcttct tttgatagca actgtttgta aaatccgttg
     8581 gaactatcgc ccataaccga aagtaccaat tgtccattta catacgctgt tctgacattg
     8641 gtcagtttaa aaaataggta ctgccgtgca ttgcgccagt cgtgcagcat ctgagtccat
     8701 gcatgcatta tggtctttgt atcatcatcg gcaggttctt tatccagtgc ttctttactt
     8761 tcatccttat tttcaattgt agctttcttc ggtacatgat tctctatatt tttttttttg
     8821 caccggctca gggcttgcgg tatccagttg ctgcagtatt gtggcaagcg ttggacgctg
     8881 taacatcttc atgatatcaa tcaaaaacat ctcaaccact gccctttcat tccctgtaaa
     8941 gcgtaactga ttttgacagt ccaataacat ggtgtaaaac aggcttaact gctcgtcatg
     9001 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatttttaa aagctgtgca
     9121 acaatgcttg caacagggat gcgtttaaaa ataaactttt gacagcgcga taaaatggtt
     9181 tcaggtatct gttgtatctc ggtagttgca aagatgaaga caacatgtgc gggcggttct
     9241 tcaagagttt ttaacaatgc attaaacgct gactttgaca gcatgtgtac ttcatctatg
     9301 atgtatacct tataacgcga cttcaatgga gcagaatgaa tattttcgcg aagctcacga
     9361 atgttttcaa caccagtatt ggatgctcca tctatttcta tgacatcaaa tgaatttccg
     9421 ttttttattt ctgtacaaaa ttcgcacact ccacagggat tgtcggttgg gccattgatg
     9481 caattgagcg atttggctac aatgcgtgcc atggttgttt tgccaacacc tcggggacct
     9541 gcaaacaaat aagcatggga tatcctgcct ttgcttatgc tgttagcaat agttcgggaa
     9601 acatgttcct gtgcaacaac atcgctgtag gtttgcgggc gccactttcg tgcaattacc
     9661 tgatatgaca tagatacctt tattgtatta ttatttcgga gagaggggga ttcgaacccc
     9721 cggtagcgtt aagctacaca cggtttccaa ccgtgctctt tcggcctctc agacatctct
     9781 ccagcaacag attataacct attttaagca taattgaaaa caatattatt gagcattaaa
     9841 aaaatataat caacgatgtc aattaagatt tcattgaaat acggtaatat gcgtatcata
     9901 atgtatggca atgtttttgc tcatggctga acacttcatt aaaaacttta tctaaagatt
     9961 tgccagtatt atatatacta tatatacatt gatgagttct acagcttttt tacattaata
    10021 aatgaggtaa ctgcatattg aatgagcaac gcaatactaa aattatcata ataaaacctc
    10081 aaagacttta acacctcata cagtttgggt tcgtgtttgg catattcccg tattgcattt
    10141 tcaagtgcaa gttctctttc ttttaattct tttgtttctt tatgtgcagt ttcaatatac
    10201 tccttaattc atttgtttta ttaacaacag atgatagaat gttcgtttgt cccacagcga
    10261 aaatcccact actgctaacg attgtgcaat gatagttcct ataacaacat acatcaaacc
    10321 ctgcaatcca tctacccgtt tttcaaggct atctattcgt tgattgactc ccttttaacc
    10381 ttcttttaaa ccttcttcaa gcctgataag cctgtctcta tcatctaatg t
//
LOCUS       DAOSOX010000084        10341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6457_length_10341_cov_8.460043, whole genome shotgun sequence.
ACCESSION   DAOSOX010000084 DAOSOX010000000
VERSION     DAOSOX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6457_length_10341_cov_8.460043"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1041)
                     /locus_tag="PLX22_07290"
     CDS             complement(37..1041)
                     /locus_tag="PLX22_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOT19741.1"
                     /translation="MSLNRREFLKVMGGTAAAVAFPGVLIQGCKRALQKASERTNVIW
                     IQAQSCSGCSVSLLNKLEPDIATVITEYISLNYHQTLCGGTGHAAIQVLENAVKTNRK
                     DFVLIVEGSIPTKSDEYCTIGDMDGRIIGAREWIQKLGANAMALVAVGTCSAFGGIPA
                     AKIRANGDNPTGATSLQTLFPDKKVINIPGCPPHPDWMVGTLLHVLLKGMPELDEYSR
                     PLMYFGTTVHEKCERLPDYKRGRFAKHWGEEGCLYLLGCLGMDTGCDIPARKWVGGMN
                     TCTGCGSGCIGCTEPPFPDYGSRGIYKHLMASNNEIESLHPQVAETVLKLKNGGVING
                     "
     gene            complement(1066..1530)
                     /locus_tag="PLX22_07295"
     CDS             complement(1066..1530)
                     /locus_tag="PLX22_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT19742.1"
                     /translation="MKPKRTLILGIGNLLQKDDGVGVHVIQYMQQHNVLLPQHVELLD
                     GGIAGFDLISHMIQFDKIMIVDALNVHDKPGSIYRFAGEHLVSKSPNVSLHELGIAEV
                     LKVLKIQGANPDIEVIGIVPENIKDLDMTLSPSVAESVPRVVDLILESVTVH"
     gene            complement(1557..1955)
                     /locus_tag="PLX22_07300"
     CDS             complement(1557..1955)
                     /locus_tag="PLX22_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT19743.1"
                     /translation="MSKSKLCLVGAVAVIFLAGCSQGTTSINPSPTDVVTVTDVNKFI
                     TDTDRQAGINATMNAMDPVKLAHKKHEDAGVKCVICHHKQGNDDRIKQCAVCHKGASG
                     EDTLHNLCINCHAKTKQGPTLCNECHIAKE"
     assembly_gap    1979..2078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2500..4764
                     /gene="hypF"
                     /locus_tag="PLX22_07305"
     CDS             2500..4764
                     /gene="hypF"
                     /locus_tag="PLX22_07305"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOT19744.1"
                     /translation="MVHYKIQVNGIVQGVGFRPFVYALATGNNCKGSVINNTEGVTIH
                     LEGDDQSIQQCIDAIKNSPPPLAKIIDIRITKLPLNGYTAFTIEESQITTCKDTFIPP
                     DVAICNECHDEFFDSTNRRYHYPFTTCTHCGPRFSIIDDIPYDRANTSMRSFPMCSNC
                     LAEYRNPLNRRFHTQPNACSVCGPEYRLCDSSGKTILTQFDAIMEQVKALLKDHIVAI
                     KSVGGYHLACDARSDRCVTLLRERKNRPFKPFAIMVSSIEFLETFCSVTAREKELLQS
                     RERPIVLIKLNKTIFSTHVAPHLTYIGVMLPYTPFQYMLFHNNRESIYIMTSANIADE
                     PIIYKDNDAFERLHTIVDYFVTYNREIIAHTDDSVMYVVDEHPFFVRRSRGYVPAPLF
                     IKKTPLHILATGGDLKNSFALARNNFVIVSQYLGDLSTPWGNDLYRKTIAHFSKIFDF
                     KPALVVSDMHPGYFTTLFAHELEEHGVQTIATQHHHAHIASVCQEHGISQPVIGIAYD
                     GTGYGTDHTLWGSEFLIADTTTFTRAAHFDYFKLPGGEHAINNPWKIGLSLLQCTGID
                     SNNFFTNEKEKFLVEEIIQKNINCPLTCSIGRLFDGVAALLGLAHHISTEAEAAMLLE
                     EEALKAYSDTCVLSIPISNSMPYGINTTWLVQEIVSMMDKGIAPATIAMRFHRAIADV
                     TVRVALMLREQFKIHSVALSGGVFHNRILLHCIVEGLRKNRFDVYTPLQLPCNDGGIA
                     LGQIAIGRDTYNGH"
     gene            4754..5812
                     /gene="hypD"
                     /locus_tag="PLX22_07310"
     CDS             4754..5812
                     /gene="hypD"
                     /locus_tag="PLX22_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOT19745.1"
                     /translation="MDIKKEAVLLSQYTLQTPVAIMEVCGTHTTEFFRTGVKDIFPQG
                     LRLIDGPGCPVCVTSNDYLDTAIAIGWQYDCIIATFGDMMKVPSSYSSLSKEKSKGLT
                     VEVVYSPMDAVQLAADNPDKKIVFLSIGFETTAPAEAVCVLEAKKRNIKNFFILPGNK
                     LTVPAVKVLLDSGEVNIDGFILPGHVSAIIGKKAWNFIATDYGKPAVVVGFEDHDLIR
                     GTLLLLNLITHANPNVVNEYTRVVRDDGNPKALEILYSVFKKGDAIWRGIGTIPESGL
                     LLNDDHAYFDALQQFTPEVPPPKEHPGCRCGELLRGVIIPTDCPLFGKACNPEHAIGP
                     CMVSSEGPCSAYYKYGRK"
     gene            5813..6811
                     /gene="hypE"
                     /locus_tag="PLX22_07315"
     CDS             5813..6811
                     /gene="hypE"
                     /locus_tag="PLX22_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOT19746.1"
                     /translation="MDIIVPGHGSGGKMMNDLINNTIKSILGSDTIQLDDSAILTVPV
                     HSIAFTTDSYTITPIEFSGGTIGSLAVNGTVNDLAVMGAVPRFISCALILEEGLPIET
                     LQRILISMKEAALKAGVAIVTGDTKIVPRGKGDRIYINTAGIGTVESSVQRRPIVPGD
                     SIIVNGTIGDHGTAIMALRNNLSFSKGLSSDCAALNHLIQDIIKHFPDAIKFMRDATR
                     GGVASILNEITKGASFGAKLYEEALPIRDEVKGVSEILGIDPLYSANEGKVVIVADSI
                     YANDIIAIMKKHPEGKDAAVIGTITSEFPEKAYMQTTIGGNRILPVLIEEQLPRIC"
     gene            6829..7059
                     /locus_tag="PLX22_07320"
     CDS             6829..7059
                     /locus_tag="PLX22_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOT19747.1"
                     /translation="MCLAIPMTVKRIEGTKAVVESMGVEKIVDIALSPDIKINDKVIV
                     HAGFVIEVLDPEAAREIEATWEEYTTLMENKQ"
     gene            7075..7911
                     /locus_tag="PLX22_07325"
     CDS             7075..7911
                     /locus_tag="PLX22_07325"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT19748.1"
                     /translation="MRTITTHDIYTEVSKLIVEANFYLPGHVQKALHAMYNRETKPLA
                     RETLSILLKNATIAQQNHIPLCQDCGSVIVFIDMGDEVHIQGNLYECINRAVADTYRA
                     HYLRPSMVQDPLFARTNTGNNTPAWIHVAIVEGSSITITVYIKGGGSENMTSLTMFTP
                     TTDVHTIIEYVVSTVKQAGPNPCPPLFLGIGIGGTADVAMLNAKKAVLRNKPHPMQKY
                     KQLEEEILQRCNETQVGPLGFGGNNTVAAVHIKEAPTHIATLPVALNLNCHSLRYGTA
                     VL"
     gene            7908..>8321
                     /locus_tag="PLX22_07330"
     CDS             7908..>8321
                     /locus_tag="PLX22_07330"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT19749.1"
                     /translation="MKSIPLTIPLSTDDIGALKAGMLITLNGILYTARDSAHKIIAEM
                     VQNNEPLPINFNNAGIFYAGPCPTPRGLPVGAIGPTTSYRMDAYTEMMLSLGVKVFIG
                     KGYRNNNIKKLLVQYKAIYCSSFGGAAAYLNQCVIS"
     assembly_gap    8322..8421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8422..8605
                     /locus_tag="PLX22_07335"
     CDS             <8422..8605
                     /locus_tag="PLX22_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT19750.1"
                     /translation="YCSSFGGAAAYLNQCVISSKPVAFEWLGPEAIVELTVNNFPAVI
                     INDIYGGDLYANLSEP"
     gene            8583..9188
                     /locus_tag="PLX22_07340"
     CDS             8583..9188
                     /locus_tag="PLX22_07340"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOT19751.1"
                     /translation="MLTFQNHDEFLHFCERIKQTLQQRPPRQIHIEKKSLKGSAVLIP
                     LLYKDNEPYLLITRRTNKVATHKGEVSLPGGGIDPVDITPLDTALRETHEEIGIKPED
                     ITVLGEFDHFISIYGFHVYTFAGIIPYPYTLTISHDEIESYTEVPLSLFVEEKYDNVD
                     YYTYEGIPYAIYYYQYNGYTIWGLTARILTDFSRKVIKQII"
     gene            9390..9668
                     /locus_tag="PLX22_07345"
     CDS             9390..9668
                     /locus_tag="PLX22_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19752.1"
                     /translation="MKKIFVPILCIIIMAIATGCLHQQLSEDEFSIIWQEYLKREFVE
                     SFDEEQSTMQRRDILNSVLKDFSINEQSFYSYCRTKHPDKFRLFDIKP"
     gene            9689..>10341
                     /locus_tag="PLX22_07350"
     CDS             9689..>10341
                     /locus_tag="PLX22_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT19753.1"
                     /translation="MIQWLKKRSFHIIVISFLAGIFIGVNIAFNVSAQEPSFKYLDFF
                     HQVYQLIRDEYVEQADSKNLFYGAIRGMIASLNDPFSRFLDEQDFSQLKEETSGEFIG
                     VGIEISAKNGDIVVVSPIEGTPAMKAGIQPGDIIYKVDNKPIKDKNINDIISIIRGKP
                     HTKVILSIIREGIDDPIDIELERLPIKLQSVNYAIIDNTNIGYLRIKVFSEDTASEV"
BASE COUNT         3087 a         2185 c         1985 g         2884 t
ORIGIN      
        1 tgtccttcta ttctggtaat tggatcaagt aataccttag ccattgatca cacctccatt
       61 cttaagtttt agtacagttt cagcaacctg tggatgcaag ctttcaattt cattattgct
      121 tgccatcaga tgcttataga tacctctgct gccataatcc gggaatggag gttctgtaca
      181 tccaatgcac cctgatccac aaccggtaca ggtgttcatg ccacccaccc atttccgcgc
      241 aggtatatca caccctgtat ccatacccaa acatcccaat aggtataaac atccttcttc
      301 accccaatgt ttggcaaatc gacctcgttt ataatcagga agcctttcac atttttcatg
      361 aacagtagtt ccaaaataca taagaggcct gctatattca tcaagttcgg gcatcccctt
      421 taataataca tgcaatagtg tgccaaccat ccagtcagga tgaggcgggc atcctggaat
      481 attaatcacc tttttatcgg ggaataatgt ttgtaatgat gttgcccctg ttggattatc
      541 accgttagcc cgtatctttg cagcaggaat gccaccaaat gctgagcatg taccaacagc
      601 aaccaatgcc attgcattag cacctaattt ctgaatccat tcccgtgctc caataatcct
      661 gccatccata tctccaatgg tgcaatattc atcggatttt gtaggtattg atccttccac
      721 aatcaataca aaatcttttc ggttggtttt aaccgcattt tccaatacct gtattgctgc
      781 atgaccggta ccaccacaca atgtctggtg atagttaagg ctaatgtatt cagttattac
      841 ggtagcaata tccggttcta atttgtttag cagtgatacc gagcatcctg aacaggactg
      901 tgcctgaatc catataacat tcgttcgttc agaggctttc tgcaatgccc gtttacaacc
      961 ctgaatgagc acacccggga aagcaactgc ggctgctgta ccacccatta cttttaaaaa
     1021 ctctcttcta tttagcgaca tgtaatatcc tccttattaa tcaggttaat gaacagtaac
     1081 gctttcaaga atcaggtcca caactctggg aactgattct gcaaccgaag ggcttaatgt
     1141 catatctaaa tccttaatat tttccggaac tattccaatg acttcaatat caggattagc
     1201 cccctgtatc ttcaaaacct taagcacttc ggcaatgcct aattcatgca atgaaacatt
     1261 gggagatttt gatactaaat gctctcccgc aaacctgtat atgctaccag gtttatcgtg
     1321 aacgtttaat gcatcaacaa tcataatttt atcaaactgg atcatatgcg atataagatc
     1381 aaatccagct ataccaccat ctaacaattc aacatgctga ggcaacaata cattatgttg
     1441 ctgcatatac tgaatcacat gcacaccaac accatcatct ttttgtagca ggttccctat
     1501 acccagtatt aacgttcgtt taggtttcat tgcacattct ttctttaaga gctttattat
     1561 tcttttgcta tgtgacactc attacataga gtaggacctt gttttgtctt tgcatgacag
     1621 ttaatacata gattatgcaa tgtatcctca ccacttgctc ccttatggca caccgcacat
     1681 tgtttaatcc tatcatcatt gccctgttta tgatggcaaa tgacacattt tacccctgca
     1741 tcttcatgtt ttttatgagc cagctttaca gggtccatgg cattcattgt agcattaatt
     1801 ccagcctgcc tgtcagtatc agtaataaac ttgttaacat ccgtaactgt tacgacatca
     1861 gttggactgg gattaatgct ggtggtacct tgcgaacatc ctgctaaaaa aataaccgca
     1921 acagcaccta ctaaacacag tttgctttta ctcacatttt acctccttaa actattgtnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg tgtcaatcta aattttttca
     2101 cattataaac aattaatgca aataatttaa tatatgcaaa tattcaatgt gtgccccaac
     2161 taccagtcat gacaaccata caacattaat tgaaactgta tactattatt gtgcaaacat
     2221 ccaatgcttt cagtctaaaa aaataacgaa cagtttcagc agtaaagtta cccatactac
     2281 attgcacatc aactaactac atagcaaagt aacctatcca ttattgcaat gatgctgtaa
     2341 tataatagaa tggatgtaat gtcaactatt atttaaaata ttataaaatt cttaaatatc
     2401 tgtattgcca ccctcaatgt aaaaaagcaa agcctttttt tattgcaaaa aagtgtgccc
     2461 attccctata aacaggtaac atgtgctaca attttaacta tggtacacta taaaatacag
     2521 gtcaacggga ttgttcaggg tgtgggattc aggccctttg tgtatgctct tgctaccggt
     2581 aataactgca aaggtagtgt tataaacaat accgaaggcg ttaccattca ccttgaaggt
     2641 gatgaccagt caatacagca atgtattgat gccataaaaa attcacctcc tccccttgcc
     2701 aaaattattg atatacgtat aacaaagctt ccattaaacg gctatactgc ctttaccata
     2761 gaagaaagcc agataacaac ttgcaaagat acctttatcc cgcctgatgt tgccatctgt
     2821 aatgaatgcc atgatgaatt ttttgatagt accaacaggc gctatcatta tccgtttaca
     2881 acatgcaccc attgcggacc gcgcttcagc attattgatg acatccccta tgacagggca
     2941 aacacatcca tgcgctcctt ccccatgtgc agtaactgtc ttgcagaata ccgcaatccg
     3001 ttaaaccgcc gctttcatac gcagcccaat gcctgcagtg tttgtgggcc tgagtacagg
     3061 ctatgcgata gttccggtaa aaccatcctg acacaatttg acgccattat ggaacaggta
     3121 aaggcattgc tcaaagacca catcgttgcc ataaaaagtg ttggcggcta tcatttagca
     3181 tgtgatgccc gcagtgaccg atgtgtaacg ctgttgcgag aacgtaaaaa ccgaccattc
     3241 aaaccctttg ccatcatggt ttcctccata gaatttttag aaacattttg ctctgtcacc
     3301 gcccgggaaa aggagctttt gcaaagcagg gaacgcccca tagtgctgat taaacttaac
     3361 aaaaccatct tcagcactca tgttgcaccg catttaacct atattggcgt tatgcttccc
     3421 tacacccctt tccagtatat gctttttcac aataatagag aaagcatcta tatcatgaca
     3481 agcgcaaaca ttgcagatga acctattatc tataaagata atgatgcgtt tgagcgtctc
     3541 catactattg tggattattt tgtaacctac aaccgtgaaa taatagctca caccgatgac
     3601 tcggttatgt atgtggttga cgagcatccc ttctttgtcc gtcgctctcg cggctatgtt
     3661 cccgcgcctc tcttcataaa aaaaactccg ctgcatatac ttgcaaccgg tggagatcta
     3721 aaaaacagtt ttgcactggc gcggaataat tttgttattg tgagccagta ccttggtgat
     3781 ctgtccactc catggggcaa tgacctttac cgaaaaacta tcgcccactt tagtaaaatc
     3841 tttgacttca aacctgcact ggtagtcagc gatatgcacc ccggctattt taccacatta
     3901 tttgcccatg aactggagga gcatggagta caaaccattg ccacacagca tcatcatgca
     3961 catatagcct cggtatgcca ggagcatggg atatcccagc ccgtcattgg tatcgcgtac
     4021 gatggcacag gctatggcac cgaccatacc ctgtggggga gcgagttttt aattgctgat
     4081 actacaacct ttactcgcgc tgcgcacttt gattacttta aactgcctgg cggagagcat
     4141 gccatcaaca atccatggaa gattgggcta tcactattgc aatgtactgg aatagacagt
     4201 aacaattttt ttaccaatga aaaagaaaaa ttccttgttg aagagattat acaaaaaaat
     4261 atcaattgcc ctcttacctg cagcattggc aggctttttg atggtgtagc agcactgttg
     4321 ggattagcac accatatcag tacggaagct gaggcggcaa tgcttctgga agaagaggcg
     4381 ttaaaagcat attctgatac ctgtgtattg tccataccaa taagcaattc catgccctat
     4441 ggcataaaca caacatggct tgttcaggag attgtcagca tgatggataa gggaatagcg
     4501 ccggcaacta tcgccatgcg ctttcaccgg gcaattgccg atgttaccgt gcgggtagct
     4561 ctcatgttgc gagaacaatt caaaatacat agcgttgcac tttccggcgg cgttttccat
     4621 aatcgtattt tattgcactg tattgttgag ggactgcgta aaaataggtt tgacgtatac
     4681 acaccattac aattaccgtg caacgatggc ggcattgcat taggacagat agctattggc
     4741 agggatacct ataatggaca ttaaaaaaga agcggtgctc ttatcacaat acacattgca
     4801 aacacccgtt gcaattatgg aggtgtgcgg cacccatacc actgaatttt ttagaaccgg
     4861 cgtaaaggat atatttccgc agggattgcg ccttattgat gggccgggat gccctgtttg
     4921 tgttacatcc aatgactatc tcgatacagc cattgctatt ggctggcagt acgattgcat
     4981 cattgctacc tttggggata tgatgaaggt accctcatcg tacagctcac tgtcaaaaga
     5041 aaaatcaaaa gggctgacgg tagaagttgt gtattcacca atggatgcgg tgcagttagc
     5101 tgccgataat cctgataaaa aaattgtttt tttaagtata ggctttgaaa caacagcgcc
     5161 agccgaggca gtttgtgtac ttgaagcaaa aaaacgcaat attaaaaact ttttcatttt
     5221 acccggtaac aagcttaccg tgccggcagt taaggtgttg ctcgactctg gcgaggtaaa
     5281 tattgatgga tttatattgc ccgggcatgt cagcgctatc attggcaaaa aagcatggaa
     5341 tttcatagca acggactatg gcaaaccagc ggttgttgta ggttttgaag atcatgatct
     5401 aatccgcgga acactgctat tgctcaattt aattacgcac gccaacccaa acgtggtgaa
     5461 cgaatatacc cgtgttgttc gtgacgatgg caatccaaaa gcactggaaa tactctacag
     5521 cgtatttaaa aaaggtgatg ccatctggcg cggtattggt actatccctg aaagtggatt
     5581 gctgctcaac gatgaccatg catactttga cgcattacag cagtttaccc ccgaggtccc
     5641 gccaccaaaa gagcatccgg gatgccgctg cggcgaactt ttgcgcggtg tgattatacc
     5701 aaccgattgt cctttatttg gcaaagcgtg taatcccgag catgccattg gcccgtgcat
     5761 ggtatcatcc gagggaccat gctcagcata ctataaatat gggagaaagt aaatggatat
     5821 cattgttccc ggccatggta gtggcggcaa gatgatgaat gatctcatca acaacacaat
     5881 caaatctatc cttggaagtg acaccatcca gttagatgac tctgccatcc ttaccgtacc
     5941 ggttcattcc atagccttca ccactgatag ctacactata acacccatcg aattttccgg
     6001 aggaactatc ggcagccttg ctgttaacgg taccgtcaac gatttagccg ttatgggagc
     6061 agtaccacgg tttatctcat gtgcactcat tctggaagaa ggattgccaa tagaaacatt
     6121 acaacgaata cttatatcaa tgaaggaagc ggcgctcaag gcaggcgtgg cgatagttac
     6181 cggagataca aagattgttc ccagaggtaa aggcgaccgt atctatataa acactgccgg
     6241 cataggcacc gtggagagct ctgtccaaag gcgacctatt gtacccggcg atagtattat
     6301 tgttaatgga actatcggcg accatggcac tgctattatg gctttgcgaa acaatctaag
     6361 cttttcaaag gggctatcgt ccgattgtgc agctttaaat catctgattc aggatatcat
     6421 caaacatttt cctgacgcca ttaaatttat gcgcgatgca acacgcggtg gcgtagcatc
     6481 aatactcaac gaaattacga aaggagcatc atttggtgct aaactatacg aagaagcact
     6541 ccccatacgt gatgaggtaa agggcgtgag tgagatactg ggcatagacc ctttgtacag
     6601 cgccaatgaa ggcaaggttg ttattgttgc cgatagtata tatgccaatg atattattgc
     6661 aatcatgaaa aaacacccgg agggaaaaga tgctgctgtt ataggcacca taacctctga
     6721 atttccggaa aaagcatata tgcaaacaac aattggaggc aacaggattt taccggtatt
     6781 aatagaggaa cagcttccac gaatatgtta acctgaaagg aggataccat gtgtctggca
     6841 ataccaatga cagttaaacg aatagaagga acaaaagccg ttgttgaatc aatgggagta
     6901 gaaaaaattg ttgatatagc gctatcacct gatataaaaa ttaatgataa ggtaattgta
     6961 catgcgggat ttgttattga ggtccttgat ccagaggcag cccgcgagat agaagcaaca
     7021 tgggaggagt atacaacact gatggaaaac aaacagtaaa ttcacgtttt tattatgcgc
     7081 acaattacaa ctcatgacat ttatacagaa gtatcaaagc ttattgttga agcaaatttt
     7141 tacctgcccg ggcatgtaca aaaggcactc catgccatgt acaaccgtga aaccaaaccg
     7201 cttgcacggg aaacactttc catcctttta aagaatgcaa ccattgcaca acaaaaccat
     7261 ataccactgt gtcaggactg cggttcggtg atagtattta ttgacatggg ggatgaggtg
     7321 catatacagg gaaaccttta tgaatgtatc aaccgtgccg ttgccgatac ctaccgtgca
     7381 cactatctgc gcccatcaat ggttcaggat ccactgtttg caaggacaaa caccggcaac
     7441 aataccccgg catggataca tgtggcaata gttgaaggaa gctcaattac cataaccgtg
     7501 tatattaaag gcggtggttc cgagaacatg acctcactca caatgtttac tccaacaact
     7561 gatgtacata caattattga atatgtggtc agcacggtaa agcaagcagg gccaaatccc
     7621 tgcccgccac tatttctggg aataggcata ggaggcactg ctgatgtggc aatgctcaat
     7681 gctaaaaagg cagtattacg caataaaccg cacccaatgc aaaaatacaa acagcttgaa
     7741 gaagaaatat tacagcgatg taatgaaacg caggtgggac cattaggctt tggaggtaat
     7801 aacactgtgg ctgcggtaca cattaaagaa gcaccaaccc atattgcaac attgcctgtt
     7861 gcattaaatt taaactgcca ttcgttgcgt tacgggacgg cagtactatg aaatctattc
     7921 cattgaccat tccattatcc accgatgata tcggtgcatt aaaagcaggg atgctcatca
     7981 cactcaacgg gattctatac acagcacgcg acagcgccca taaaataatt gcagaaatgg
     8041 tacaaaacaa tgaaccattg cccattaatt ttaataatgc tggtatcttc tatgcaggac
     8101 catgccccac accaagaggg ttgccagttg gcgctattgg acccacaaca tcataccgta
     8161 tggatgccta tactgagatg atgctttcgt taggggtaaa ggtattcatt ggcaagggtt
     8221 accgcaataa caatataaaa aaacttctgg tacaatacaa agctatttac tgctcaagct
     8281 ttggcggagc tgctgcctat cttaaccagt gcgttatcag cnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nttactgctc aagctttggc ggagctgctg cctatcttaa
     8461 ccagtgcgtt atcagctcaa aacctgttgc atttgaatgg ttagggcccg aagctatagt
     8521 tgaacttaca gtaaataatt tcccggctgt tattatcaat gatatatatg gtggtgacct
     8581 ttatgctaac ctttcagaac catgatgaat ttttgcattt ttgtgaaagg attaaacaaa
     8641 cattgcaaca aagacctccc cgtcagattc atattgaaaa aaaaagtttg aagggttcag
     8701 cggtactcat tcctctgctc tataaggata atgaaccata cctgcttatt acccgacgaa
     8761 caaataaggt tgccactcat aaaggcgaag tgtcgttacc cgggggaggt attgaccctg
     8821 ttgatataac accgcttgac actgcattgc gtgaaacaca tgaagagata ggtataaagc
     8881 ctgaagatat aacggtgctg ggcgaatttg accactttat ctccatttat ggatttcatg
     8941 tatacacgtt tgctggaata attccttatc cctacacact taccatcagt catgatgaga
     9001 tagaatccta taccgaggta ccgttatccc tttttgtaga agaaaaatat gacaatgttg
     9061 actattatac ctatgagggc attccctatg ccatttatta ttatcagtac aatggttata
     9121 caatctgggg attaacagca cgtattttaa cagacttttc acgtaaagtt attaaacaaa
     9181 ttatataaat atctttacaa ttttttacca tcatactact attgaaaatt gaaaatgaaa
     9241 aaacatcata gccacatctt tcatagggta agcaactaaa atgaagctac tttactacta
     9301 tttttttgtg cttacaatgg ttgcatacag tatagttttt ggtgtgctgt attaatgaat
     9361 gtttataaaa aattgtcagg attgtaacaa tgaaaaagat ttttgtacct atactgtgta
     9421 tcattatcat ggctattgca acagggtgtt tgcatcagca actttctgaa gatgaatttt
     9481 ccataatctg gcaggaatat ttaaaacggg aatttgttga aagctttgat gaagaacaat
     9541 caaccatgca acgaagagat attttaaact ctgtattgaa ggatttttca atcaatgagc
     9601 agtcatttta cagttactgt cgcacaaaac acccggacaa attcaggctt tttgatatca
     9661 aaccataaat aagtaaagtg aggaatatat gatacagtgg ttaaaaaaaa ggtcatttca
     9721 tataattgtc atttcatttt tagcaggaat attcattggt gtgaatatcg cattcaatgt
     9781 atcagcacag gagccctcat ttaaatattt agattttttt catcaggtat atcagcttat
     9841 ccgtgatgaa tatgttgagc aagctgactc taaaaatctt ttttatggag ctatccgggg
     9901 gatgattgct tcgttaaatg atcctttttc acgattttta gacgagcagg atttcagtca
     9961 gttgaaagag gaaacaagtg gtgagttcat tggtgtgggg attgaaatat ctgctaaaaa
    10021 cggcgatatt gttgtcgttt caccaattga agggaccccg gcaatgaagg caggaattca
    10081 accaggagat attatttaca aggttgataa caagccaatt aaagataaaa atattaatga
    10141 tataatatca ataatacggg gtaaacctca tacaaaagtt atactgtcaa ttatccgtga
    10201 aggtattgat gatcctattg atattgagct ggaacgtctt cccattaagt tacagtcagt
    10261 taattacgca attattgata atactaatat tgggtatctc aggataaagg tctttagcga
    10321 ggataccgca tccgaagtta a
//
LOCUS       DAOSOX010000085        10028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6714_length_10028_cov_9.405495, whole genome shotgun sequence.
ACCESSION   DAOSOX010000085 DAOSOX010000000
VERSION     DAOSOX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6714_length_10028_cov_9.405495"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PLX22_07355"
     CDS             complement(<1..1329)
                     /locus_tag="PLX22_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT19754.1"
                     /translation="MKKDIDFFKRYPLLPLRDMVIFPHMVIPLFVGRDISIKALDKAM
                     KGDRLLVLVAQVDSQIANPKKEDVHDVGTLSEILQLLKLPDGTVKVLVEGIDRVYVSQ
                     FETDDDGITVSIKNIERPYEADSKVEALKRNLIDTFEKYIKLNKKVPQEALTTVMQIE
                     DISHLADVIASYISMRVEFKQAILELGNAIERIEKLIELINGEIEIMYIEKKIQGKVR
                     KQMEKTQKEYYLNEQMKVIKKELGQGEDLADEVEELHKSIEEAKMSKEAKDKALKELK
                     RLEKMPPLSAESAVVRTYIEWLRDVPWFKKTRDNRDIDHAMHVLNEDHYGLEEVKERI
                     IEYIAVRHLSNKLKGPILCFVGPPGVGKTSLGRSVARALGRRFVRMSLGGVRDEAEIR
                     GHRRTYVGALPGRIIQQMKKAGVVNPVFLLDEVDKMSMDFRGDPSSALLEV"
     gene            complement(1401..2669)
                     /gene="clpX"
                     /locus_tag="PLX22_07360"
     CDS             complement(1401..2669)
                     /gene="clpX"
                     /locus_tag="PLX22_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT19755.1"
                     /translation="MANKKKDNTKPEVIHYCSFCGKSQDLVRKLVAGPNVYICDECIE
                     LCNEILEEDWALDGGDLLTNNLPKPHEIKAILDEYVIGQEYAKKVLSVAVYNHYKRIT
                     SNFNLRKGDVELDKSNILLIGPTGSGKTLLAQTLAKILKVPFAIADATSLTEAGYVGE
                     DVENILLRLIQNADGDIKRAEIGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKI
                     VEGTIANVPPQGGRKHPHQEFISIDTKNILFICGGAFVGLEKIIHARIGNKTMGFGAE
                     VKSVKDIGKDNILEKMIPDDLIKYGLIPEFVGRLHVNAVLQELDKHTLRDILTKPRNA
                     LTKQYQKIFGFENVELEFTSEAIEAIAQIAIERETGARGLRSVLEEIMLDLMYEIPSK
                     KTIAKVVINDDVVLHRKPPDIIQRTTEKSA"
     gene            complement(2703..3293)
                     /gene="clpP"
                     /locus_tag="PLX22_07365"
     CDS             complement(2703..3293)
                     /gene="clpP"
                     /locus_tag="PLX22_07365"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOT19756.1"
                     /translation="MSYLVPIVVEQTARGERAYDIYSRLLKDRIIFIGDAIDDHIASL
                     VIAQMLFLEAEDPDKDIYLYLNSPGGIVTSGLAIYDTMQYVKPDVATICLGQAASMAS
                     LLLAAGAQGKRSALPNSRIMIHQPMGGFQGQASDIAIHAKEILKIKEKLNEIYTIHTK
                     QPLEKIEKDVDRDYFMDAQEAVEYGLIDKVIRKDSR"
     gene            complement(3392..4735)
                     /gene="tig"
                     /locus_tag="PLX22_07370"
     CDS             complement(3392..4735)
                     /gene="tig"
                     /locus_tag="PLX22_07370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT19757.1"
                     /translation="MEIKESKLDNARIRLDIEVPGDRIDAEYARVLKELQKKAEIKGF
                     RVGKAPIDLVEKKYSDYVLGETSENIVRSAYVETIEQKGYTPVSQPVFDFEKVEKGKP
                     FSFKIEFDIPPTIELGQYKGSHVEQKVVKVTDEDVEAEIETMREKMATITKKEGDTIK
                     VQKGDQVTLKMKRIDDKSKEEIDAAAFTSHNLIVGKSKESWAIDDDIIGMEKDEEKEI
                     KVKYPKDYEVEDLKGQKVTYLVKVEEIFEFKLPELNDDFAKSVGEYNSVDDLKNRIRE
                     NLETYVKSQTLNETKNKILEMIVENSKFDIPQSLIQKEMYAIFRRLQSRFGNVGNDIN
                     EFASIFGINPEELSKSIMDEADKNIKNAMALSKIAELENLKVSEEQFQKFVKSVAERN
                     TVSEEEVVKTVEEKGNKEEIEGDLILDSAYDFIYEHADIKMSKPITFQEYVNGKK"
     gene            complement(4977..6614)
                     /locus_tag="PLX22_07375"
     CDS             complement(4977..6614)
                     /locus_tag="PLX22_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19758.1"
                     /translation="MDKKIVNRNNVYFYTFLFLLVFVLRIPALYHTIVDIDEAAFAEF
                     ANKWINGGIPYVHMYDNKPLLVYFFYYIVFYFFGINNLFAVHAVTIILVYCCAIIIYK
                     FIQNIRGIDEAKAASLFFIFLMHIYEPKYIATNTETLFTLPILISFISYYLFIINKWK
                     TNIALVTSGIFAGISFFINYKAGVILIVYGIFAIFSITKATNKKREFVKQLKDMIVIA
                     TGFLMVVAGATSYLYCNNALDDFITLGFLYNFKYIQSGMTTVPIVKAIARFIIFIVCS
                     LPLWLIAGRHAIHLLKEKQYTPITLISILWLLAGCFSVLLGWRAYGHYFIQLVPPLAI
                     LGGLLIKDITLSLLKKIYIYFIIMMILFTASRINIPLTYHYLGDSNSLSDYAYKNVAH
                     AVTEITAPDDTIFVWGSGAVAYIYANRRCASKIIIADYISGRQFGVSNEDMQANVNAY
                     IQLLRKQFIAEVMKNPPAVVIDTSPSGYYGYDKFPLKNFHALYDFINNNYRFAKRIDM
                     MDIYVLKNITMRHSTIIPVRSNGSKAFGKNQECVAPK"
     gene            complement(6599..8296)
                     /locus_tag="PLX22_07380"
     CDS             complement(6599..8296)
                     /locus_tag="PLX22_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOT19759.1"
                     /translation="MFLNNTVKIFYKLFNKNSFMVFVCICFSTIILRLPSVIIDFIHV
                     DVLTSYLLAKRDLLGLSYSMNKGWLYHQLMKFSINYIGDTPSSFHFIGLLFILTTMYG
                     IYILAKKMYDSKTGLFAAFLYGTVISCYHTEYLAFNGEIFYNLFNVFALYCYYVCVFE
                     KKRIYIVLLVINIVASYLIKFQGIFILPIILTHFIFIYPLSLNTNDYRRFYKNLFIAI
                     ASALVTVATFLIAGYVYFGFDFIDYIYKAIMGKYTYVANRGFNIFSILIKLLWRVYQF
                     SIFHAFFWFPGIILIAKFIYDFKNKKIDYKTLFILYVSVCFFILVFSGLGRLSVHYFI
                     PIMPSIAILSSKQILYVFSERGKRMVFMQLMFTIVFFFAWHYKDLYIYTCNPNLKHNE
                     SRLTEIFRIAIIGSYGEYLLPHKSLLRAIEYINKNHPGSTMLIWPMGTEIDYFTTGES
                     VIPSYWLNERALYAVVQREKGNEHYIHEYENYIIQLIIQYKPDLFVDVGSTDMIKKVM
                     VYRKKGDPLFYFNIHATPIIHFGHFASLDDFPAIIRYLNEHYTFNGYFGKSRIWIKK"
     gene            8523..9614
                     /locus_tag="PLX22_07385"
     CDS             8523..9614
                     /locus_tag="PLX22_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT19760.1"
                     /translation="MDNCISIIVPTYKEADNISVLVESIDKAMRSANLNYEIIIVDDN
                     SNDGIVEKVKQLNKQKYPVAIKVRVHERGLSSAVIEGFKLAKGNIYLVMDADLSHPPE
                     KIPDIVQPIISGKAEFVVGSRFVEGGSAEHFNIYRKLNAWISKMLAMPFTKVKDPMAG
                     FFAFDARLLTPEILPILNPLGFKIGLEMIVKCKPKTVVEIPITFKERLYGKSKLSTRE
                     QVLYLLHLKRLFHYKYPSMYQFLFFSIIGASGMIVDLTTVFITYEVLHIKFIIARAIG
                     FIIALTWNFFLNRKLTFPDSPQKNIFKQYRNFFLVCSIGGLFNWFISVFLYWHYSYFN
                     QFYLLAAFIGILGGLLINFFGSKFIVFGK"
     gene            complement(9618..>10028)
                     /locus_tag="PLX22_07390"
     CDS             complement(9618..>10028)
                     /locus_tag="PLX22_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19761.1"
                     /translation="LNSGMRIMKKDVVKKYFKILPDGFSFTSTITLAMLSNGCHVKYI
                     PINYNHRKGKSKIRPFYDTLNFFRLIIRTVMFFDPLKIFLPISLPLFGGGIILTLLQA
                     ILYQNIHTISVLLTLSGLNILTVGMIAEMIANKD"
BASE COUNT         3329 a         1837 c         1397 g         3465 t
ORIGIN      
        1 aacttccaat aacgctgatg aaggatcacc cctgaaatcc attgacatct tatcaacctc
       61 atcaagcaaa aatacgggat ttaccacacc tgcttttttc atctgttgaa ttattctgcc
      121 tggtaatgct ccaacatagg tgcgtctatg accacgaatt tctgcttcat cacgaactcc
      181 acccaatgac atacgcacaa accttcttcc taaagctcgt gctactgacc ttcccagcga
      241 tgttttccca actcccggtg gcccaacaaa acatagtatg ggtcctttca atttatttga
      301 taaatgacgt acagcaatat attctataat cctttcttta acctcttcca gcccataatg
      361 atcttcattg aggacatgca ttgcatgatc tatatctcta ttatcacggg tttttttgaa
      421 ccaaggcaca tcacgtaacc attcaatata tgttcggaca accgctgact ctgctgacaa
      481 aggaggcatc ttctctaaac gttttaattc tttcagtgct ttgtcttttg cctctttaga
      541 catctttgct tcttcaatag atttatgtaa ttcttcaact tcatcagcta aatcttcacc
      601 ctgccctaat tcttttttta taactttcat ttgctcattg agatagtact ctttctgagt
      661 tttttccatc tgtttgcgaa ctttaccctg tatcttcttt tcaatataca ttatctcaat
      721 ttcgccattt ataagctcaa tgagtttttc tattctttca attgcgttac ctaattctaa
      781 aattgcctgt ttaaattcta ctcgcattga aatgtacgag gcaatcacat cagctaaatg
      841 agaaatatct tcaatttgca tcactgtagt taaagcttct tgtggtactt ttttattaag
      901 ctttatatac ttttcaaatg tatcaataag atttcgtttt aatgcttcta cttttgaatc
      961 tgcttcatat ggtcgctcaa tgtttttaat tgaaacggta ataccatcat catctgtttc
     1021 aaactgtgaa acataaactc tgtcaattcc ttcaactaac actttaaccg taccatcagg
     1081 cagttttaaa agttgtaaaa tttcagacaa agtcccaaca tcatgcacat cttctttttt
     1141 tggattggca atctgggaat ctacctgggc aacaagaaca agcaatctgt cgcccttcat
     1201 tgctttatcc agagctttta ttgaaatatc cctgccaaca aataacggaa taaccatatg
     1261 cggaaatata accatatccc tcagcggtaa taaaggatat cttttaaaga aatctatatc
     1321 ttttttcata gtttctccct ataaggataa taagctttca attaataaaa tactaaaaaa
     1381 attctctttt gaaaacataa ctaagcactt ttttctgtag ttcgttgaat aatatcagga
     1441 ggtttacgat gcaaaacaac atcatcatta ataactactt tggcgatagt ttttttcgat
     1501 ggaatctcat acataagatc taacatgatt tcttctagta ctgatctcaa tccccttgca
     1561 ccagtttcac gttcaattgc tatttgagca attgcttcaa ttgcctcact ggtaaattca
     1621 agctcaacat tttcaaatcc aaatattttt tgatactgtt ttgtaagagc atttcgaggt
     1681 tttgttaaaa tatcccgtaa agtatgttta tcaagttctt gtaacactgc attaacatgt
     1741 aatctaccaa caaattcagg aataagccca tatttaatga gatcatcggg tatcatcttt
     1801 tcaagtatat tatccttgcc aatgtccttg actgatttca cttcagcacc aaatcccatc
     1861 gttttattgc caatccgcgc atggataatt ttttccaaac caacaaatgc accgccacat
     1921 ataaataaaa tattcttagt atcaattgat ataaactcct gatgggggtg ctttctgcca
     1981 ccttgaggag gaacattggc gatagttcct tcaacaattt ttaacagtgc ttgttgaacg
     2041 ccttcgccgg aaacatccct tgtaatggat ggattttcgg actttcgtga aattttatca
     2101 atttcatcaa tataaattat accaatctct gcccgcttta tatcaccatc agcattttga
     2161 attaagcgta ataatatatt ttcaacatcc tcacccacat atcctgcttc ggttaatgat
     2221 gttgcatcag caatggcaaa gggaactttt aaaatttttg ctaatgtctg agcaagcaat
     2281 gtttttccac ttcctgttgg gccaattaag aggatattgc ttttatctaa ttcaacatca
     2341 cctttacgca ggttaaagtt gctggtaatc cttttataat gattatacac cgccacagag
     2401 agaactttct tggcatattc ctgacctata acatattcat caaggattgc tttaatttca
     2461 tgcggcttgg gcaaattgtt tgttaaaaga tcccccccat caagagccca atcttcctcc
     2521 aaaatttcat tgcaaagctc tatacactcg tcacagatat agacatttgg ccctgcaact
     2581 aactttctca ctaaatcctg acttttacca caaaacgaac aataatgaat aacttctggt
     2641 tttgtattat cttttttctt attagccata aatcctcatg ttcaatatac tatatacaat
     2701 acttatctac tatcttttcg tataacttta tcaattaatc catactcaac agcttcctgt
     2761 gcatccataa aatagtccct atcaacatct ttttcaattt tttctaaagg ctgttttgta
     2821 tgaatagtat aaatttcatt taacttttcc ttaatcttca aaatttcttt cgcatgtata
     2881 gcaatatcag atgcctgtcc ctgaaaccca cccataggtt gatgaatcat tatacgcgaa
     2941 ttaggcaatg ccgaacgctt tccttgagca cctgctgcta aaagtaacga tgccattgaa
     3001 gcagcctgac ctaaacaaat cgttgcaaca tcaggtttta catattgcat tgtatcgtag
     3061 atagccaatc cagatgtaac aattccacct ggcgaattta agtatagata gatatcctta
     3121 tcagggtcct cagcttccag aaataacatc tgtgcaataa ccagacttgc tatatgatca
     3181 tcgattgcat cgccaataaa aataattcga tccttcaata atcgcgaata tatatcatat
     3241 gcacgctcac cccgtgctgt ttgttctact acaattggta caagatagct catagggtta
     3301 cctcattatt atcatttttt tggatatgtt ataagtatag tttagcattt ctttattgct
     3361 attcaataaa ttattattgt tcagcaactg cttatttttt tccatttaca tattcttgaa
     3421 atgtaatagg ttttgacatc ttgatatctg catgctcata tataaaatca tatgcggaat
     3481 caagaatcaa atcaccttca atctcttctt tattaccttt ttcttctact gttttgacaa
     3541 cctcttcttc agaaacagta ttacgctcag caacagattt tacaaatttt tgaaattgtt
     3601 cctcagacac ttttaagttc tccaattctg ctattttgct caatgccatc gcatttttaa
     3661 tgtttttatc agcttcatcc ataatcgatt tggatagctc ttccggatta attccaaata
     3721 tagaagcaaa ctcatttata tcattgccaa catttccaaa tcgtgactgc aagcgtctaa
     3781 aaattgcata catttctttc tgtataagag attgaggaat atcaaatttg ctgttttcaa
     3841 ctatcatctc taatatttta ttcttcgttt catttaatgt ttgtgacttt acatacgtct
     3901 caagattttc acgaatacgg ttttttaaat catcaaccga attatattca ccaacgcttt
     3961 tagcaaaatc atcattgagt tcaggtaatt taaattcaaa aatttcctca acttttacca
     4021 ggtaagttac tttttgacct tttaagtctt ccacctcata atctttaggg tatttaactt
     4081 ttatctcttt ttcttcatct ttttccatcc ctattatatc atcatcaata gcccatgatt
     4141 ccttgctttt cccaactatt aaattatgcg aggtaaatgc agcagcatca atttcttctt
     4201 ttgacttatc atcaatacgt ttcattttca aagtgacttg atcgcccttt tgcactttga
     4261 tagtatcacc ttcttttttg gtaatggttg ccatcttttc acgcatcgtt tcaatctcgg
     4321 cttcaacgtc ttcatcggtt acttttacta ccttctgttc aacatggctt cctttatatt
     4381 gacctaattc aatagtagga ggaatatcaa attcaatttt gaaggaaaag ggctttcctt
     4441 tttcaacctt ttcaaaatca aaaactggct ggcttactgg cgtatagccc ttctgctcaa
     4501 ttgtttcaac ataagctgaa cgaacaatat tttccgaagt ttctcctaaa acgtaatcag
     4561 aatatttttt ttctaccaga tcaataggcg ctttaccaac tctgaatcct ttaatttcag
     4621 cttttttttg caactctttt agtactcttg catattctgc atcaatacga tcacctggaa
     4681 cttcaatatc aagacgtatt cgagcattat caagtttaga ctctttaatt tccaaggtta
     4741 actctcctaa tatatgtaat tttatttaaa attcaggttt atatgtgctt gataaatatt
     4801 ataaaaaaca ctcctcatag tgtataaaca aaacgtaaac gttaatgatt atatatgact
     4861 ataaatatag cacattgatg catttataag cgatattgta actattaatg atatatttta
     4921 ttctgtaatg tcaggcgtca aatatttttt aataataatt attcggcgca tataaattat
     4981 ttgggcgcaa cacattcttg atttttccca aaagcttttg agccattact cctgacaggg
     5041 atgatagtag agtgacgcat ggttatattt ttaagcacat aaatatccat catatcaatt
     5101 cttttagcaa agcgatagtt attgttaatg aagtcgtaga gtgcatgaaa attttttagc
     5161 gggaatttat catagccgta ataacccgaa ggtgaagtat caataacgac agcaggagga
     5221 tttttcataa cttcagcaat aaactgcttt ctcagaagtt gaatatacgc atttacattt
     5281 gcctgcatat cttcatttga tactccaaac tgtcgccccg aaatatagtc tgcaataatg
     5341 atttttgatg cgcatcgtct attggcatag atatacgcaa cagcaccgga accccataca
     5401 aatatggtat catcaggagc ggtaatttca gttactgcat gtgcaacatt tttatacgca
     5461 taatctgata atgaattact atcgcccaga taatggtatg ttaatggaat atttatcctt
     5521 gatgctgtaa atagtatcat cataataata aaatatatat atattttttt taacaacgat
     5581 agtgttatat ccttaattaa taaacctcct aatattgcaa gtggtggaac caattgaata
     5641 aagtaatgcc catacgccct ccatcccaaa agtaccgaaa aacatccagc caacagccat
     5701 agtatactta taagagtgat tggcgtatat tgcttttctt tcaataaatg aattgcatgc
     5761 ctgcctgcta tgagccatag aggtaaactg catactataa aaataataaa ccgtgctatt
     5821 gcttttacta tgggtacagt tgtcattccg ctctgtatat atttaaaatt atataaaaaa
     5881 cccaatgtaa taaaatcatc caatgcattg ttacaataca aatagcttgt tgccccagcc
     5941 actaccatta aaaatccagt agcaataaca atcatatctt ttagctgttt aacaaactct
     6001 ctttttttat tggttgcttt tgttatacta aaaattgcaa aaataccata aacaattaaa
     6061 ataaccccag ctttataatt aataaaaaaa gatattccgg caaaaatacc agatgtaact
     6121 aaagctatgt ttgtcttcca cttattgata ataaaaagat agtaacttat aaatgaaata
     6181 agtattggta atgtaaacag tgtctcagta ttggttgcga tatactttgg ttcatatata
     6241 tgcattaaaa aaataaaaaa caagctggct gctttagcct catctattcc tctaatgttt
     6301 tgtataaact tatagataat gattgcacag caatagacaa gtataatggt aacagcatgg
     6361 acagcaaaaa gattgttgat tccaaaaaaa tagaaaacta tgtaataaaa aaaataaact
     6421 aacaatggtt tattatcata catgtgtaca tagggaattc caccattaat ccatttattg
     6481 gcaaattctg caaaagcagc ttcatcaata tcaactattg tatgatacaa tgctggaata
     6541 cggagaacaa ataccagtaa aaacaaaaaa gtataaaaat atacattgtt tctatttact
     6601 atttttttat ccatatcctg cttttcccaa aataaccgtt aaatgtataa tgttcattaa
     6661 gatagcgtat gattgctgga aaatcatcta aactggcaaa atgtccaaaa tgaattattg
     6721 gcgttgcatg aatattaaag taaaataaag gatcaccttt ttttctatat accattacct
     6781 tctttatcat atcagtagaa cccacatcta caaaaagatc aggtttatat tggataatta
     6841 attgtataat ataattttca tattcatgta tatagtgttc attccccttc tctctttgaa
     6901 ccactgcata cagagccctt tcattcaacc agtatgaagg gataacggat tcgcccgtag
     6961 taaaataatc aatttcagta cccattggcc agattaacat tgtgctgcca ggatgatttt
     7021 tattaatata ttcaatagca cgcaataaac ttttatgcgg caacaaatat tcaccgtacg
     7081 aaccaataat agcaattctg aatatctcgg ttaacctact ttcattatgc tttaaattgg
     7141 gattgcatgt ataaatatag agatctttgt aatgccatgc aaagaaaaat acaatggtaa
     7201 acatcagttg cataaatacc atgcgtttcc ctctttcaga aaacacataa agtatttgct
     7261 ttgaagaaag gattgcaata gatggcatta tagggataaa ataatgaact gataaacgac
     7321 ctaaaccaga aaaaacaagg ataaaaaagc atactgaaac atatagtata aataatgttt
     7381 tgtaatctat ttttttattt ttaaaatcat atataaattt agctattagg ataatgccgg
     7441 gaaaccagaa aaaagcatga aaaattgaaa attgatatac tctccacaac agcttaatca
     7501 aaattgagaa aatattaaaa cctctattag caacataggt atacttgccc attattgctt
     7561 tgtagatata atcaataaaa tcaaaaccaa aatatacata tccagcaatt aaaaaggttg
     7621 caacggtaac caatgcacta gcaatggcaa tgaaaagatt tttgtaaaaa cgtctgtaat
     7681 cattggtatt taatgataat ggatatataa atataaaatg tgttaaaatg ataggcagga
     7741 taaagattcc ctggaattta atcaaatacg atgcaacgat attgattaca agtaatacaa
     7801 tatatattct ttttttttca aatacacata catagtaaca atacagtgca aaaacgttaa
     7861 acaaattata aaatatttct ccattaaatg caaggtactc agtatgatag caggatataa
     7921 ctgtgccata taagaatgct gcaaataacc ccgttttaga atcatacatt ttttttgcta
     7981 aaatataaat gccatacatt gtcgttaaaa taaaaagcaa tccaataaaa tgaaacgatg
     8041 atggtgtatc accaatatag ttaatagaaa atttcataag ctgatgatac aaccatccct
     8101 tattcatgct gtatgatagt cccaataaat cacgctttgc cagtaaatat gatgttaaaa
     8161 catcaacatg aataaaatca ataatgaccg acggtaaccg taagattatt gtactgaagc
     8221 agatacatac aaataccatg aaggagtttt tgttaaataa tttataaaaa attttaacgg
     8281 tattgtttag aaacaatgca tttctccatt gacatgatta catattatgc caaaatgatg
     8341 ttaaattttt aaatgtaccg tcaacataat tttttttatg tatccaataa aattgtattg
     8401 taaaaaacac agtgcattaa tttatactct ccatggtgca atatattatc aagtttgtca
     8461 tattctttgt aaggtataat agcaatatta tgctatgcat tatttattga aaggaattgt
     8521 atatggataa ttgcatttca atcatagtac ctacgtataa agaagcagat aatatctctg
     8581 ttcttgtaga atctattgat aaagcaatga gatcagcaaa tcttaactat gagataataa
     8641 ttgttgatga taattcaaat gatggcattg tagaaaaagt taaacaattg aacaaacaaa
     8701 aatatccagt tgccattaaa gtaagagttc atgaacgcgg tttatcttca gcagttatag
     8761 aaggatttaa attagctaaa ggaaatatat atctggttat ggatgccgat ttaagccatc
     8821 ctcctgaaaa aatacctgat atcgttcaac ctattatcag cggcaaagct gaatttgttg
     8881 taggaagcag atttgtagaa ggcggttcag cagaacattt taatatatat cgtaaactca
     8941 atgcatggat ttcaaagatg ttagccatgc cttttacaaa ggttaaagat ccaatggcag
     9001 gtttctttgc gtttgatgcc aggctattaa caccagagat acttccaatc cttaaccctc
     9061 ttggttttaa aattggactg gagatgatag ttaagtgcaa accaaaaacg gttgtagaaa
     9121 ttccaataac gtttaaggaa cgtttatatg gtaaaagtaa gctatcaacc cgcgagcagg
     9181 ttttgtattt actgcattta aaacggctat tccattataa atatccatcg atgtatcaat
     9241 ttttattttt ttcaataatt ggcgcatcag gtatgatagt tgatttaaca actgtcttta
     9301 ttacttatga agttttacat ataaaattta tcattgcccg tgccattggc tttataatag
     9361 cgttaacatg gaatttcttt ttaaatagaa aattaacctt ccccgattct cctcaaaaaa
     9421 atatttttaa gcaatacaga aacttttttc ttgtatgcag tataggtggt ttgtttaact
     9481 ggtttatttc ggtattttta tactggcact attcatactt taaccaattt tatttacttg
     9541 ctgcttttat agggatttta ggaggattat tgataaactt ctttggttca aagtttattg
     9601 tttttggtaa ataatatcta atctttgttg gctatcattt cggcaatcat tcctacagta
     9661 agtatgttta agcctgatag tgtcaaaagt actgaaatag tgtggatatt ctgatacaga
     9721 attgcctgca aaagcgttag tataatgcca cccccaaaaa gaggaaggct aatgggcaaa
     9781 aatattttaa gcggatcaaa aaacattaca gtacgtataa taagcctgaa aaagtttaac
     9841 gtatcataaa aaggtctaat ctttgatttc cctttcctat ggttatagtt aatgggaata
     9901 tattttacat gacatccatt agatagcata gctaaagtga tagttgatgt aaaagaaaat
     9961 ccatcaggaa gaatcttaaa atattttttt acaacatcct tcttcattat ccgcataccc
    10021 gaatttaa
//
LOCUS       DAOSOX010000086         9877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_6836_length_9877_cov_9.992160, whole genome shotgun sequence.
ACCESSION   DAOSOX010000086 DAOSOX010000000
VERSION     DAOSOX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_9877_cov_9.992160"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1956)
                     /locus_tag="PLX22_07395"
     CDS             complement(259..1956)
                     /locus_tag="PLX22_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT19762.1"
                     /translation="MSEFVIKPKVDETQEFIEIANNFSNPLEIVREAISNSYDAKASQ
                     IKISFDVVSEYGESILKIVIEDNGAGMDKDGLQSFFDLGNSLRRNDPSSIGEKGHGTK
                     VYLNSKKIELITKKDKTEYSASMDEPYRKLHNREIPEVKVKENSPHQMSSNGSIITIW
                     GYNNNRRDKFSHEILRDYIMWFTKHGAIENAFVQSGNQVILFLKGLGKDDFEEIKQGH
                     YFPDDSDDINNLFTNYKMQAPDYYCKKIIRSGKLKNFPEINYHAIFAIEGKYVKYGYN
                     SMLRRRGYNAPNGAYTIQDRYGLWLCKDFIPIQRKNDWITYKGSEYTKFHAFFNCQSF
                     RLTANRGSIENTPSEVLLDIKEEVQTIYDEILKSDDWTLIEWLEEESNARQTIEKEKN
                     NFSWRLKKINNSNIATYKGLTLVEPERESGVFALFLLLSTIEPDLFPFQIVDYDTHNG
                     IDVIAKGDKNTPIFNAKLFYVEFKRTLEKGFNHSFENIHSIVCWDTKVKHDEILHDLS
                     NEERKLQIVSPKNQNDYTRYFLDNPRKAHRIEVFVLKQYLKEKINIDFTPRTKDSLV"
     gene            complement(2207..2992)
                     /locus_tag="PLX22_07400"
     CDS             complement(2207..2992)
                     /locus_tag="PLX22_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT19763.1"
                     /translation="MKVKGNKHNQLSFSVTDSHVHIGQFNDFYFSPEFVVETLRKVGV
                     SRWIVSSTSSIENNYDKVKNEFKKIIDIAPLESYPALWVTPEMLNKSPDLNFFFNEIN
                     YYAIKIHGVANTWYPYRKNLNIVFEIAKKRNIPIILHTGGQKQTYASAYGSICSKHST
                     VKVILAHGRPLDEAISIINANSNVFVDISFMSVENVDTLIHSVNPEKILFGTDFPMDL
                     IFYQNESIIDRYKNRLDSLISSIGLENFYKISNLNIEQLFFKK"
     gene            complement(2973..4412)
                     /locus_tag="PLX22_07405"
     CDS             complement(2973..4412)
                     /locus_tag="PLX22_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19764.1"
                     /translation="MFIKQSNDTFIRKYNNIGYIYNQRTKHDRIYDETGSIFLSKLTR
                     TPKNFNEITEELQSIFTSANRAEIENDLKDFILELEKDKFVIVGESKNDVESKDYLFS
                     YSFEDPKKSTYSFLNPEKYQTILSTTDFFDNYLRNNPQIIDMEMELTSRCNERCLHCY
                     IPHENKLNDIDAQLAMDILCQMKELGGINISLSGGEPFLHENFISILQSARKNDLSIS
                     ILSNAVLLSNEIMSCIKDANISQIQISLYSMNQKEHDKITQLQGSWKKTIDNIERLIS
                     ENIPVQISCPVMKINQFSYIDVLKWGYDKKIKVNTDFIMMGRTDFSTDNLKFRLNSIE
                     TTQLIKDIIRNDKEYIEQLDIEPKSNKFDDYKNKPVCGVGISNICMVSNGNFYPCSGW
                     QSYILGNAYKNSLKEVWENSENIKFLRSITNSSFPQCLSCEARDYCSMCMVRNFNESN
                     GDMFKVNKNFCEVAFITKKEVENYKSKRK"
     gene            5299..6324
                     /gene="purM"
                     /locus_tag="PLX22_07410"
     CDS             5299..6324
                     /gene="purM"
                     /locus_tag="PLX22_07410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT19765.1"
                     /translation="MTYKDSGVDVEGGNKFVKRIRSLVKDTFTKEVLTDIGGFAALFD
                     ASFKQYKHPVLVSGTDGVGTKLKIAQMMNIHNTIGIDAVAMCVNDIIVTGARPLFFLD
                     YISCGKLHEDVLVNIIQGIASGCKMADCALIGGETAEHPGIMQPDDYDIAGFSVGVVD
                     KQNSINGNTINPGDIIIGIASSGVHSNGYSLVRKLFFDIKKYTIETKFHELSKPLGAT
                     LLEPTKIYVKPILKALESIAIKGMVHITGGGFYENIPRILPKNTAAYIDKSSFPIPPI
                     FSIIQHEGNIDEREMFTTFNMGIGFICIVSKKDADALITGLTRHGEKAYSIGEIKSRL
                     DSPVIIS"
     gene            complement(6292..>7149)
                     /locus_tag="PLX22_07415"
     CDS             complement(6292..>7149)
                     /locus_tag="PLX22_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOT19766.1"
                     /translation="KFKLSNQTFLRWCSEELKSLMVSLIIGVPLLLLFYFFIKLTSNW
                     WLYFAIVVFIVAIVLAKVAPVLIFPLFYKFTPIDNEQLKNTLNDLMQEHGLRLKGIYS
                     FNMSKNTNKANAAFTGLGKSRRIILSDTLINEFSINEIKSVFAHELGHYIHKHILKNI
                     IISGFIIIISFYICSYFYEITLPSAGYTNRFDIAALPLLIFYLSVFSLLLMPVLNTLS
                     RYYEKQADMYALATTNDPDSFISTMEKLAAMNLSTKEQHPLIEFFMYSHPTIAKRIAL
                     ANDYRRIQS"
     assembly_gap    7150..7249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7277..7843)
                     /locus_tag="PLX22_07420"
     CDS             complement(7277..7843)
                     /locus_tag="PLX22_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT19767.1"
                     /translation="MDELIENIGKRIQYYRKKNDTTLKELADKIKATPSLISQIEHGK
                     ANPSLGTLKAIADVFGVPIGLFFENDIKRTTSSPVIRKNTHRKLITNGNVAYTLLNPD
                     SEDMEVILIEFPPQVATGEEHYQHDGYEVGYVIKGELTVVLDDNEYNLQLGDSIAFRS
                     IRPHTIKNKSSQNAVAVWVNLIPWIFVK"
     gene            8279..8983
                     /locus_tag="PLX22_07425"
     CDS             8279..8983
                     /locus_tag="PLX22_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19768.1"
                     /translation="MSYHVNKNILNCNTLLIAVIITLSFIFLLPQYLYALAIVDTYHQ
                     IQTTFEGLSKFLNSIYTVVSTITSILGYRAIVLFFAIAVTSAGLSAIGFPKGKTTFFI
                     AFIIDNILWYTWNKSFGIDYTATVLTMLKTNAVIVFPYIIFIIIKRYALQIKHFFIQI
                     FHMVLPSHYKKKITKNALSETINSLHSAYATMMSSIMNDLIHSNDTINLSQDTIATIE
                     HTETILKKIKETGDKQ"
     gene            8980..9324
                     /locus_tag="PLX22_07430"
     CDS             8980..9324
                     /locus_tag="PLX22_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19769.1"
                     /translation="MNFTSNRYDDIVIINIAGIGEYGDDVKLIKYMEKLLDNKLYIVA
                     VNFSDVDTINSAAVAALVYLLKYGDEHGFDVAFFGANDKIIMILERALPKYAINIFTE
                     EEFYKRFNIPNK"
     gene            9333..9635
                     /locus_tag="PLX22_07435"
     CDS             9333..9635
                     /locus_tag="PLX22_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19770.1"
                     /translation="MIFTKREEDNRCIFIINEQLNPRTLKELIEEMHNFLQNDSRDAI
                     IDMSNVEIVDSTILAGFMTLYNNFKNNHKKFRLINTNSYVKRIIELASLDTFLLEE"
     gene            9636..>9877
                     /locus_tag="PLX22_07440"
     CDS             9636..>9877
                     /locus_tag="PLX22_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT19771.1"
                     /translation="MKLAPNGFKRRKGALVLIIMDGVGAGTNDDGNAFFLARTPVFDS
                     LSKSCPYTLLKAHGTAVGLPSDADMGNSEVGHNALGA"
BASE COUNT         3218 a         1578 c         1512 g         3469 t
ORIGIN      
        1 gacgaaggtg ccaaagcaac gcttcgtcat ttttctcctt tagatttcag caaaagatgt
       61 aggaataact tgtaataaat gattttcaaa accgggcaat gcgtaaaatg ttttgcttat
      121 gcaaagtcgc aacagcctta ccaccgcgat ttggcgcagc cggaacgagc aacagcgagt
      181 gaagcgtata agcttaatta tacgcagaaa gcgcttttta taaaatgatt caggcgaaac
      241 ccggatgttg agctctcatt atacaagaga atcctttgtt cttggagtaa aatcaatatt
      301 aattttttct tttaaatatt gtttaagaac aaaaacttca atacgatgtg cctttctagg
      361 attatcaaga aaataccttg tataatcatt ttgatttttt ggtgaaacta tttgcaattt
      421 tctttcttca ttgcttaaat catgtaatat ttcatcatgt ttaactttag tgtcccaaca
      481 aacaatgcta tgaatatttt cgaacgaatg attaaaacct ttttcaagtg ttcttttgaa
      541 ttcaacgtaa aataatttag cattaaaaat aggtgtattt ttatctcctt ttgcgattac
      601 atcgatacca ttatgtgtat cataatctac tatttgaaaa ggaaacaaat ctggttcaat
      661 tgtagacaat aataggaaaa gtgcaaaaac accactttct cgttctggct ctacaagggt
      721 taatcccttg tatgttgcga tgttagaatt attgattttt tttaagcgcc aagaaaaatt
      781 atttttttct ttttcaatag tttggcgtgc atttgattct tcttcaagcc attctattaa
      841 agtccaatca tcacttttta aaatttcatc atatattgtc tgtacttctt ctttgatatc
      901 aagcaatact tcggatggtg tattttcaat tgaacctcta tttgcagtta acctgaaaga
      961 ttgacaatta aaaaaagcat gaaatttagt gtattcagag cctttatagg taatccagtc
     1021 atttttcctt tgaatcggta taaagtcttt acataaccat agcccgtacc tatcttgaat
     1081 agtataagca ccattaggag cattataacc gcgacgtctt aacatcgaat tatatccgta
     1141 cttcacatat ttaccttcaa ttgcaaatat ggcgtgataa ttaatttccg gaaaattttt
     1201 taattttcca cttctaataa tttttttaca ataataatca ggtgcttgca tcttataatt
     1261 tgtaaataaa ttgtttatat catcgctatc gtcaggaaaa taatggcctt gtttaatttc
     1321 ttcaaaatcg tctttcccca atccttttaa aaataatatt acttggttcc cagattgcac
     1381 aaatgcattt tcaatagcac cgtgtttagt aaaccacata atataatccc gcaatatttc
     1441 atgagaaaac ttatctcgcc tattgttatt atatccccaa attgttatta tgctaccgtt
     1501 agacgacatt tggtggggac tattttcttt aacttttact tcgggtattt ctctattatg
     1561 taattttcga taaggttcat ccattgatgc tgaatactct gttttgtctt tcttagttat
     1621 aagttcaatt tttttgctat ttaaatatac ttttgttcca tgtccttttt caccgataga
     1681 gcttggatca tttctccgta aggaattacc aagatcaaaa aatgattgga gaccatcttt
     1741 atccattcca gctccattat cttcgataac aattttgaga attgattcac catattcaga
     1801 aactacgtca aaagaaattt ttatttgaga cgcttttgca tcataagaat tactaattgc
     1861 ttctcgtaca atttctaatg gatttgaaaa attatttgct atttcaatga attcttgagt
     1921 ttcatcaact ttcggtttaa tcacaaattc tgacatgtta attcctccta taatttattt
     1981 tttgcgaggc caggacggcc gagcttattg aaaatattgc gctttttgcg tacaacgttc
     2041 ccggcgtatg cgacgttttc accgtagcgc agcgaaggtg aaaatgtggc gaagcccaag
     2101 cgaacgtagt gagcgcagcg catcacgccg tgttatatgc cgtttgctcg ccattcaaca
     2161 taaaggctgc cgaagccacg gatggcgcag gcaatttgga taatagctat tttttaaaaa
     2221 ataattgttc aatatttaaa ttagaaattt tataaaaatt ttcaagacca atagaactaa
     2281 taagtgaatc taatctattt ttatatctgt caattattga ttcgttttga taaaaaatta
     2341 aatccattgg aaaatcagta ccaaaaagta ttttttcagg atttacacta tggattaatg
     2401 tgtctacatt ttcaaccgac ataaatgata tatcaacaaa aacattagaa ttagcattaa
     2461 taatactaat cgcttcatct aatggacggc catgggcaag tattaccttg acggtactat
     2521 gttttgagca aatactacca taagctgagg cataagtttg tttttgtcct cctgtatgca
     2581 gtattattgg tatatttctt tttttagcaa tttcaaaaac tatatttaga ttttttctat
     2641 atggatacca tgtatttgct acaccatgga ttttaattgc atagtaattg atttcattga
     2701 aaaaaaaatt taaatctgga gatttattta gcatttcagg tgtaacccac aaagctgggt
     2761 aactctcaag tggcgcgata tcaataattt ttttaaattc atttttgact ttatcataat
     2821 tattttcaat agaagaagtt gaagaaacaa tccatctgga gaccccgact ttcctcaaag
     2881 tttcaacaac aaactcaggt gaaaaataaa aatcattgaa ctgaccaata tgaacatgag
     2941 aatcagttac tgaaaaactt agttgattat gtttatttcc ttttactttt ataattttcg
     3001 acctcttttt tcgttataaa tgcaacttca cagaaatttt tattaacttt aaacatatca
     3061 ccattacttt cattgaaatt tctcaccata cacatagaac aataatctcg agcttcacag
     3121 gaaagacact gcgggaagga ggagtttgta attgatctta agaattttat attttcagag
     3181 ttttcccata cttctttaag agaattttta tacgcatttc ctaaaatata gctttgccat
     3241 cctgaacatg gatagaaatt tccattagaa accatgcaga tattgcttat tccaacacca
     3301 caaactggtt tatttttata atcatcaaat ttatttgatt taggctcaat atctaattgt
     3361 tcaatatact ctttatcatt tcggataata tccttaatta gctgagtagt ctcaattgaa
     3421 ttaagtctaa atttcaagtt atctgttgaa aagtctgtac gccccatcat gataaaatct
     3481 gtattaactt ttattttttt atcgtatccc cattttaaca catcgatata cgagaattga
     3541 ttaattttca ttactgggca acttatttga acgggaatat tttcacttat taagcgctca
     3601 atattatcaa tggttttttt ccaggatcct tgcaattgtg taattttatc atgttctttt
     3661 tgattcatgc tatataatga gatttgtatc tgacttatgt tcgcatcttt gatgcatgac
     3721 atgatttcat tgctcaataa taccgcattg ctcaatattg aaattgaaag atcattcttc
     3781 cttgcagatt gaagaattga gataaaattt tcatgtagaa aaggctcacc tccagataaa
     3841 gagatattaa taccccccaa ctctttcatt tggcataata tatccattgc taattgtgca
     3901 tcaatatcgt ttagtttatt ttcatgtgga atataacagt ggagacatct ttcgttgcag
     3961 cgagatgtta gttccatttc catatcaatt atttgcggat tatttctcaa ataattatca
     4021 aaaaaatccg tagtactgag aatggtttga tatttttcag gatttaaaaa gctatatgtt
     4081 gatttttttg gatcttcaaa actataggag aataagtaat cttttgattc gacatcgttt
     4141 tttgattcac ctacaattac aaatttatct ttttctagct ctaaaataaa atcctttaga
     4201 tcattttcaa tttctgctcg attagccgat gtaaaaattg attgtaattc ctctgttatt
     4261 tcattaaaat ttttaggtgt tcttgttaat ttacttaaaa agattgaacc agtttcatca
     4321 taaattctat catgctttgt tctttgatta taaatgtaac caatattatt atattttctt
     4381 ataaaagtat cattagattg ttttatgaac ataaaatttc tcccgaaaaa aaattggcag
     4441 gcaatctatt cgttaattta ttgcctgcca aaataatgaa tcatgtaaga ttattttcta
     4501 atctcacagg acctccaaca agtacctgtt ttttctgggc aacctgcgga atagctccca
     4561 gttggttggt tttttgcaag tacttctggt tttttgtaag acataacgtc ctcctctaga
     4621 aagtttgttt tattatacaa taatataaat tattgtcaat ttttttaagc cgccacggat
     4681 ggcggcgccc ccataaagtt ggcgagcaaa tagcatataa ctcattcata tatgaactac
     4741 ttcgcttttt atcctgtttt gtgagtatat acgaagtatt tcgtctatac tgattctggc
     4801 tgaatttccc tgattctttt tatctttcaa aagtttccac ccacctcctt tcttgaggtt
     4861 taatgtatct aaagaactcg ttatatttcc taataattat gctataattt ggtggatgac
     4921 ataaaaagta atatatactc agataaaaaa gtttaagcaa aacaatttaa atacgttgga
     4981 tttgtcacca tgaaataaaa gataacgtga atataacaaa atagtcaagc ataaaatcta
     5041 ttagatgatt tgtctctgaa cagaattttg cgggataaga gttaagaaac tttttcaggc
     5101 agctatcgaa cgctaaattg atgggaaacc tggaaaatgc aaatcgttta aagatgcaca
     5161 tgaaaagcaa ttggtatacc attaataaat aagatttcca cagaaacatc gaataattaa
     5221 ttgacataca ataggcatca tgtcacaaga taaccctgga agataaaaag caaataaact
     5281 caagaggtaa ccatgggaat aacctataaa gattctggtg ttgacgttga aggcggcaac
     5341 aaatttgtta aacgaatacg ttcactggta aaggacacat ttacaaaaga ggtattgact
     5401 gatattggtg gttttgcagc cctttttgat gcgtcattta aacagtataa acatccggta
     5461 cttgtttccg gcactgacgg tgtaggcaca aagttaaaaa ttgcccagat gatgaatatt
     5521 cacaatacca ttggcattga tgcagtagca atgtgtgtca acgatattat tgtaacaggt
     5581 gccagacctt tatttttttt agattatatt tcatgtggaa aactacatga agatgtactt
     5641 gtcaatatca tacagggcat tgcttcagga tgcaaaatgg ctgattgtgc cttaataggc
     5701 ggcgaaacag cagaacatcc gggcataatg caacctgatg attatgatat agctggattc
     5761 agcgttggtg ttgttgacaa acagaatagc ataaatggaa atacaattaa tccaggtgat
     5821 atcattatag gcatagcttc atctggtgtt cattctaatg gctattcgct tgtgcgaaaa
     5881 ttgttctttg atattaaaaa gtataccatt gaaacaaaat ttcatgaact atctaagcca
     5941 ttaggcgcaa ctctgcttga accaactaaa atttatgtta aacctatcct caaagcgctt
     6001 gaatcaatag ctatcaaagg catggtacat attaccggcg gtggatttta cgaaaatatc
     6061 ccccgtatac ttcccaaaaa cactgctgct tatattgata aaagttcatt tcctattcct
     6121 ccaatattct caattataca gcatgaaggt aatattgatg aaagagagat gtttacaacg
     6181 tttaatatgg gtattggctt tatttgtatt gtgtcaaaaa aagatgccga tgcgcttatt
     6241 acaggtttaa cccgtcatgg tgaaaaagca tatagtattg gcgaaataaa atcacgactg
     6301 gattctcctg taatcattag ctaacgcaat acgttttgca attgtaggat ggctatacat
     6361 aaaaaattca atgagcggat gctgctcctt tgttgacaga ttcattgcag ctaatttttc
     6421 catagttgaa ataaatgaat caggatcatt ggttgttgca agtgcataca tatcggcttg
     6481 cttttcatag taccgcgata gtgtattcaa tactggcatt aacaacaagc taaatactga
     6541 aagataaaaa attaataaag gcaacgctgc aatatcaaat ctgtttgtat atcccgcact
     6601 gggcaaggta atttcataaa aatatgaaca tatataaaat gatataataa tgataaaacc
     6661 gctgataatt atatttttaa gtatgtgttt atgaatataa tgtcctaatt cgtgggcaaa
     6721 tactgatttg atttcattga ttgaaaattc attaatcaat gtatcactca gaataattct
     6781 tcggctttta cccaatcccg taaaagctgc atttgccttg tttgtatttt tgctcatatt
     6841 aaagctatag attcctttta atctcaaccc gtgttcctgc attaaatcat tcaatgtatt
     6901 ttttaactgt tcattatcta ttggtgtaaa tttataaaat aacgggaata tcaaaaccgg
     6961 tgccaccttt gccagaacta tcgcaactat aaaaacaact atcgcaaaat ataaccacca
     7021 gtttgatgtt aattttataa aaaagtagaa tagcaataat aatggcacgc caataatcaa
     7081 tgaaaccatc aatgatttca attcttcact gcaccaccgc aaaaaagttt gattggaaag
     7141 tttaaatttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgatagctcc
     7261 ttttaacaat tgcacattat ttaacaaaaa tccatggaat taagttaacc catactgcta
     7321 ctgcattttg cgatgattta ttttttattg tatgaggtct gatgcttcta aaagcgatac
     7381 tatcacccaa ttgcagatta tattcattgt catccagaac aacggtaagc tcgcccttta
     7441 taacataacc tacctcatag ccatcatgtt gatagtgctc ttctccggta gccacttgtg
     7501 gtgggaactc aatcaatatt acttccatat cttctgaatc aggatttaac aatgtatatg
     7561 ctacatttcc atttgtaatt aatttacgat gggtattttt acgtataact ggtgaagatg
     7621 ttgtacgttt aatatcattt tcaaaaaaca atcctatggg tacgccaaac acatcagcta
     7681 ttgctttgag tgttccaagt gatggattag ctttcccatg ttctatctga cttatgaggc
     7741 ttggagtagc ttttatttta tcagctaatt cttttaatgt tgtatcattc ttttttcgat
     7801 aatattgtat tcgtttacca atattttcta tgagctcatc cactataagt tttcctttat
     7861 aaatgtaata ccagcgtttt atatccagtg atttaaatat aatttaaatt agttattttg
     7921 tctattaatt tttataaatt cttgattaca atttattgga tcatttatgt taaaatattt
     7981 gacaatttaa aaaagattaa attaaaatgg ataaaatatt attttttgta aaaaagtaat
     8041 ttaaatattg atagttactg tgtttcctga tataaagcag ttttgagatt ataattgcag
     8101 ttaaaatgga gcaaaaaaat atcattataa ataatgatac aactgaaact tttgttttta
     8161 cacccatatt tgaatacgat agtaataaat atgcttttta tattatgtat tatatgagaa
     8221 agcattattc aatgtgacgg gtattacttt aaataattat agtacagagt atagtgcaat
     8281 gagttatcat gtaaataaaa atattctgaa ttgcaacaca ttactgattg cagttattat
     8341 tactttatcc tttatttttt tattacctca gtacctttat gcccttgcaa tcgttgatac
     8401 atatcaccag attcaaacta catttgaagg attatcaaag tttttaaaca gtatatatac
     8461 tgtagtatct accataacat caattttggg ataccgtgcc attgtactct tttttgccat
     8521 tgcagtaaca tcagcagggc tttctgcaat aggatttccc aaagggaaaa caacattctt
     8581 tattgcattt ataatagata atatattatg gtatacatgg aataaaagct ttggcataga
     8641 ttatactgct acagtgctga caatgcttaa aacaaatgct gtaatagtat ttccgtatat
     8701 tatatttatt attataaaac gatatgcatt gcagataaag cactttttta tacagatttt
     8761 tcatatggta ttgccatctc attataaaaa gaaaataaca aaaaatgctc tgtctgaaac
     8821 aatcaattca ttacatagcg cgtatgcaac aatgatgtcg tcaatcatga atgatcttat
     8881 tcatagcaac gatactatta atctttcaca agatactata gcaactatcg aacataccga
     8941 aactatcctt aaaaaaatta aagaaaccgg agataaacag tgaattttac atccaatcgt
     9001 tatgatgata ttgttattat taatattgct ggcattggcg aatacggtga tgatgtaaaa
     9061 ttaattaagt atatggaaaa attacttgat aataaattat atatagtagc agtaaatttt
     9121 tcagatgttg atactataaa ttcagctgct gttgctgcac tggtttatct tttaaaatat
     9181 ggtgatgaac atggttttga tgttgctttt tttggcgcaa atgataaaat tataatgatt
     9241 ctggagcgtg cactacctaa gtatgcaata aatattttta ctgaagaaga gttttataaa
     9301 cgtttcaata ttccaaacaa atgaggtgag ccatgatttt tacaaaaagg gaagaagata
     9361 atcgttgtat ttttataatc aatgaacaat tgaatccgcg aaccttaaag gaattgattg
     9421 aagaaatgca caatttttta caaaatgaca gcagggatgc tataattgat atgtcaaatg
     9481 ttgaaattgt agattccact atactggctg ggtttatgac tttatataat aactttaaaa
     9541 ataatcataa aaagtttaga ttaattaaca caaatagtta tgtgaagagg attattgaat
     9601 tggcatcact tgatactttt ctgctggagg agtagatgaa attagcaccc aacggtttta
     9661 aacgtagaaa aggagccctt gtattgatta ttatggatgg agttggtgcc ggtactaacg
     9721 atgacggcaa tgcttttttt ttagcacgaa cacctgtttt cgattcattg agcaaaagct
     9781 gtccctatac cctgctcaaa gcacatggta cggcagttgg gctaccatcg gatgctgata
     9841 tgggaaactc agaggttggg cacaatgcat tgggagc
//
LOCUS       DAOSOX010000087         9510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_7159_length_9510_cov_8.628768, whole genome shotgun sequence.
ACCESSION   DAOSOX010000087 DAOSOX010000000
VERSION     DAOSOX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_9510_cov_8.628768"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLX22_07445"
     CDS             complement(<1..498)
                     /locus_tag="PLX22_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT19772.1"
                     /translation="MKYITGNILESKTQALVNTVNIVGVMGKGIALQFKKVFPENYKK
                     YADACKKGEITIGKLFITKENTLYGEKLIINFPTKKDWRKPSEYEYIEKGLDELIKII
                     EEYKITSIALPPLGAGSGGLVWSKVKTIIEEKLKNIDIEILVYEPTKDIKEKLKKERI
                     KLTDAR"
     gene            complement(518..1177)
                     /locus_tag="PLX22_07450"
     CDS             complement(518..1177)
                     /locus_tag="PLX22_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOT19773.1"
                     /translation="MKTKNLINIELFRMIHINNVPHILKYGITHRYSKNSNPDYENIG
                     DISLIDTRENKKINVTNGGSTIIKEIKLGNYIPFYFGVRMPMLYVIQHGGNFVEKPIP
                     PSDIVYIVCDLEKIIKLSQEYYFTDGHATDNLTTFYDSSKINEIKNILDWQAINAQFW
                     GGEENLTIKWGKQAEFLVKGDLPVTIIKRYICYNDEAKSRLMSFGILGENIVIDQSAY
                     Y"
     gene            1494..2708
                     /locus_tag="PLX22_07455"
     CDS             1494..2708
                     /locus_tag="PLX22_07455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT19774.1"
                     /translation="MSNLNRHSIKGILVVCLIVLFTCITEADEQQGNVTGTSNFKKPA
                     TQRDYWPTKEWKVKKPEDVGMNSQDLKKMELYAFSRLQPESERKGIRTDSVIIVKNGY
                     IVYEKYAGEFTRNKVHITWSVSKSFTNALFGIAIKEGKLSLSDPAWKFYPSLNRGQHK
                     NITVDHLMRMSSGLYSNETYEASPLKSTVNAMLFTIGHTDMAAYAASQDLEANPGEKW
                     EYASPTPNLLMAMLKNTMSAREYEQYPWEKLFNVIGMKKVVWERDVAGTFVGSSYIFT
                     TSQDMARFGYLFLNDGIWEGKRLLPQGWVAYSTTIAPAYYTTKLSKSDMADPAYGALW
                     WLNRDIPEKNMKRAFPDAPDDMFIAMGHWGQYIFIIPSLDMVIVYTGDNRDDTFNINT
                     FLKLIIDSISKR"
     gene            2713..3027
                     /locus_tag="PLX22_07460"
     CDS             2713..3027
                     /locus_tag="PLX22_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19775.1"
                     /translation="MKKIMKILVMILFIIIIIIGIVTITNWNDINNFRPMVSGAFSKF
                     MCSCLFVEQRSEEQCLQWSKLDLPVSSYSVDHNEKSVTASALGKTSKAHYTNDRFGCT
                     LQ"
     gene            3032..4018
                     /locus_tag="PLX22_07465"
     CDS             3032..4018
                     /locus_tag="PLX22_07465"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOT19776.1"
                     /translation="MLINNHSYTFFLAPMAEVSTPALRKVIKQFCGEVVLYSEMLASG
                     AINAKAPHNEPLVQRHGFDTPFFYQIVGNNPAIMANAASILQDYGCDGIDINMACSAP
                     HIVKAGCGAQLLVDMRHARDIVKSCRKAVKNLLTVKMRAGYHDIDMDYLKQFIVMLQD
                     EGVNAITLHPRAAKWGFSRSAKWDVIDEIVKHAAIPIIGNGDITTPQLAMQRLHQTRC
                     AGIMIGRAAVQQPWIFALCNALYNNNPLTVTIDHAALWIDVLTNIQTMLPQKLHKSRA
                     HRFCFYFAKNCTFGHELFTIIRKLSAIDDIIKTINDYYMRNPMDQNKHYRVP"
     gene            4313..5209
                     /locus_tag="PLX22_07470"
     CDS             4313..5209
                     /locus_tag="PLX22_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT19777.1"
                     /translation="MYYPSYLTLYHTGELKKRIDALQNILTCCTLCPHRCRIDRRIKT
                     GKCRTGVMAIVATYHAHFGEESCLVGSHGSGTIFFANCNLSCIFCQNWDISQMPNGKP
                     VSPEQLADVMIRLQQKGCHNINLVSPTHVAAQIVEALPYAIERGLTIPLVYNSGGYDA
                     VETLQLLDGIIDIYMPDFKYMDETVAKHLSGAPDYPHHATAAIREMHRQAGDLVIEHG
                     IAKRGLLIRHLVIPENSARSDLVFKEIAKLSIDTYINIMAQYHPAYRAREHPALGRYI
                     TLEEFNQALQWAKAAGLWRFDR"
     gene            5257..6324
                     /locus_tag="PLX22_07475"
     CDS             5257..6324
                     /locus_tag="PLX22_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT19778.1"
                     /translation="MLHSSSKLINLFDLVNDAIIIIDKNHNVIFNNEKGQIFVKSDGK
                     TLLLENETIINLVNTLQPDAEVIGEHISLKVKKRIHHYTLNAIAINDDNSFLIAFVIT
                     DTTRLHKLHQQQQQLISQIRKNYLDRFESLKQLADSIAHEVRNPLVSIGGYANLLIRK
                     CDEGIDHNEAKKYLQYIVDNAERLNYLTQQIEEMGDLKRVNLAAYSITKFMQEQESTF
                     IAAASQFNKKLQLNIESEKNYTIYIDYDKLRFAITQIIEFVAEHTKAENIVLHCYGTS
                     YELIIHLQCITDIIAEEDLHYLFDPFYTTRSNVETFNLCIAQRIIMLHGGIITSSLTR
                     KTLHFKIALPQDKRLTHDNGK"
     gene            complement(6301..7587)
                     /gene="hemL"
                     /locus_tag="PLX22_07480"
     CDS             complement(6301..7587)
                     /gene="hemL"
                     /locus_tag="PLX22_07480"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOT19779.1"
                     /translation="MITSKSKELYAQACKYLPGGVNSPVRAFRSVGGHPLYFKLGKGS
                     HIIDEDGNEFIDYCMSWGPLILGHADNDVVKAIQQIAENGTSFGAPHAAEIDIARLVV
                     DSFPGMQKVRFVNSGTEATMSAIRLARGFTGNSKIIKFDGCYHGHADFLLVAAGSGLA
                     TFGMPDSAGVTKGNAKDTIVIPYNDVDALHQVLSHNDDIACCIIEPVCCNYGLILPED
                     DYLQKVRDLCTQYHVLLIFDEVITGFRLARGGAQERFGVTADITTLGKIAGGGLPVGV
                     YGASHEIMSKVAPDGPVYQAGTLSGNPIAMAAGIATLQKLVPSTYQKLEQLGQYFEDA
                     MQPVVKRFGGRFLFVRFASIFALYFTNADNITSVDMVKECNMQQFAQFHREMLERGIY
                     LSPSGYEVGFLSIAHTKEDIDTTVTAIDESLAVVMS"
     gene            complement(7603..8580)
                     /gene="hemB"
                     /locus_tag="PLX22_07485"
     CDS             complement(7603..8580)
                     /gene="hemB"
                     /locus_tag="PLX22_07485"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOT19780.1"
                     /translation="MMHPYYRPRRLRENEIIRDLIAETKPDYNKLIMPYFVIEGAGIT
                     QAIESMPGIQRLSIDNLLKQLDYDVKLGVRAIILFGIPYDKDEKASGAYDENGIVQKA
                     CREIKKQFPELLIITDVCLCEYTSHGHCGMVKGNQIDNDSTLELLAKTAVSHARAGAD
                     IIAPSDMMDGRVETIRQELDDNGFITIPILSYAAKYASAFYGPFRDAAGSAPQFGDRR
                     SYQMDYRNAHEALKEVNLDIDEGADIVMVKPALSYLDIIYRVKQSVELPVCAYNVSGE
                     YSMVKAAGTLGFGDEAKLVREIMTSIFRAGADMVISYHTCDILKNRWFD"
BASE COUNT         2988 a         1933 c         1684 g         2905 t
ORIGIN      
        1 tcttgcatct gttagcttta ttctttcttt ctttaacttt tctttaatat cttttgtcgg
       61 ttcatatacc aatatttcaa tatcaatatt cttcagcttc tcttcaataa tagttttaac
      121 cttactccag accaatccac cactgccagc gcccagaggc ggcaaagcaa tgcttgtgat
      181 cttatattct tcaataattt taattaattc atctaaacct ttttcaatat actcatattc
      241 tgagggtttt ctccaatcct ttttagtcgg gaaatttata ataagttttt caccgtaaag
      301 cgtattttct ttagtaatga ataatttacc tatagttatt tcaccttttt tgcatgcgtc
      361 tgcatatttc ttgtaatttt caggaaatac tttcttaaat tgcaaagcaa tgcctttacc
      421 cattactcca acaatattta cagtattaac aagagcctgt gttttacttt caaggatgtt
      481 ccctgtaata tatttcatca tacccccctc tctattatta ataataggca ctttgatcaa
      541 ttacaatatt ttcccctaat atcccaaaac tcatcaacct gcttttagct tcatcattat
      601 agcaaatata tcttttgatg atggttacag gcaaatcacc ttttaccaaa aattctgctt
      661 gtttgcccca tttaattgtc aaattttcct ctcctcccca gaattgagca ttgatagcct
      721 gccagtcaag tatgtttttt atttcattta ttttgctact atcataaaaa gtggtcaggt
      781 tatcagttgc atgaccatcg gtaaaataat actcttgact caattttatg attttttcta
      841 agtcacaaac aatatagaca atatcactcg gtggtattgg tttttctaca aagttgccac
      901 catgctgaat tacgtataac ataggcatcc ttacaccaaa ataaaaaggt atataattgc
      961 ctaactttat ttctttgatt attgtgctgc ctccattagt gacattgatt tttttatttt
     1021 ctcttgtgtc aatcaaagaa atatctccaa tattttcata atccggatta ctattctttg
     1081 aatatctatg agtaatgcca tatttcagaa tgtgtggtac attattaata tgtatcattc
     1141 gaaataattc tatgttgatt aaattttttg ttttcataca cacactttca attccactca
     1201 ggcattgtta tgctttacca taccatctat agtttggatt ttttatgata aacatcagcc
     1261 tttttcattg caatgtaaaa atataaatac tatacccggc aatgaaaaaa gtcaacacga
     1321 atgtggtaaa aaatcatatc tgtctgtatt atcgcatgtg aatattgtaa gcaaaatagc
     1381 agtcaaaaat attatttttt atgtattgca taaataactg ttttactgat agtgtagtac
     1441 taatagtgta gtactaatag tgtagtacta ataaaaaatt aatggggggt gccatgagca
     1501 atttgaatag acacagtatc aaaggtattc tggttgtttg tttaattgta ctatttacct
     1561 gcatcacaga agcagatgaa caacagggta atgttactgg aacatcaaat tttaaaaagc
     1621 cagctactca acgggactac tggccaacaa aagaatggaa ggttaaaaaa cctgaagatg
     1681 tgggaatgaa ctcacaggac ctaaaaaaaa tggagttgta tgcgttttca cgattacaac
     1741 ctgaatctga acgcaagggc atacgaactg atagcgtcat cattgtaaaa aatggctata
     1801 tagtatatga aaagtatgcc ggtgaattta ccagaaacaa ggtgcacatt acatggtcag
     1861 tatcaaaatc atttaccaat gccctttttg gcattgcaat aaaagaagga aaattatcct
     1921 tgagtgatcc tgcatggaaa ttttatcctt cgttaaatcg cggacagcat aaaaacatta
     1981 ccgttgatca ccttatgcgc atgagctccg gattgtattc aaatgaaacg tatgaagctt
     2041 caccgcttaa atcaacggta aatgcaatgc tgtttaccat aggacatacc gatatggctg
     2101 catatgccgc ttctcaggac ctggaagcaa atcccggtga aaaatgggaa tatgccagtc
     2161 caaccccaaa tcttttaatg gctatgctta aaaataccat gagtgcaaga gaatatgagc
     2221 aatatccatg ggaaaagctc ttcaacgtta ttggtatgaa aaaagttgta tgggaacgcg
     2281 acgtggcagg aacatttgtc ggatcttcat atatcttcac cacttcgcag gatatggcac
     2341 ggtttggcta tctttttcta aacgatggta tatgggaagg caaacgctta cttccccagg
     2401 gatgggttgc atattcaaca actatcgccc cggcatacta tacaaccaaa ctttcaaaaa
     2461 gtgatatggc tgatccagcg tatggcgctt tatggtggct taaccgtgat attccggaaa
     2521 aaaatatgaa acgagcattt cccgatgctc ctgacgatat gttcatagcc atgggacatt
     2581 ggggccagta tatctttatt ataccttctc ttgatatggt tatagtatat accggtgata
     2641 accgggatga tacatttaac atcaatactt ttttaaaact tataatcgat agtattagta
     2701 aaaggtgata ccatgaaaaa aattatgaaa atactcgtta tgatactgtt tattataata
     2761 atcatcattg gaattgtgac gataaccaat tggaatgata tcaacaattt tagaccaatg
     2821 gtttccggtg ctttttctaa attcatgtgt tcatgcttat ttgttgagca aaggtctgaa
     2881 gagcaatgtc tgcaatggtc aaaacttgac cttcctgttt catcatattc ggttgaccat
     2941 aatgaaaaga gtgtaacggc ttcggctctt ggtaaaacaa gtaaagcgca ctataccaat
     3001 gaccgttttg gatgcacatt gcaatagcta aatgcttatc aacaaccata gctatacatt
     3061 cttccttgct cccatggcag aggtgagtac acctgcactg cgcaaagtca taaagcaatt
     3121 ttgcggtgag gttgtactct actctgagat gcttgcaagc ggtgctatca atgcaaaagc
     3181 accccacaat gaaccactgg tacaacgcca tgggtttgac acaccttttt tttatcaaat
     3241 agttggcaac aatcctgcta tcatggcaaa tgccgcctcc atcctgcagg attatggctg
     3301 tgatggcatc gacatcaata tggcatgcag tgctccccat atcgttaagg ctggatgcgg
     3361 tgcgcaattg cttgtagata tgcgccatgc aagggatatt gttaaaagct gccgtaaagc
     3421 ggtaaaaaac cttttaacgg ttaaaatgcg tgcggggtat catgatatag atatggacta
     3481 cttaaagcag ttcattgtga tgctgcagga tgaaggggtt aacgccatta ccctgcatcc
     3541 acgagctgca aagtggggtt ttagccgtag cgcaaagtgg gatgtcattg atgaaattgt
     3601 taagcatgct gcaatcccca taattggcaa tggtgatatt acaacgccac agcttgccat
     3661 gcagcggtta catcaaacac ggtgcgccgg cattatgatt ggcagagcag ccgtacaaca
     3721 accgtggatt tttgcactgt gcaatgctct ttacaataac aatccattaa cagtaactat
     3781 cgaccatgct gctctctgga tagatgtact taccaacata caaacaatgc tcccgcaaaa
     3841 acttcataaa agcagggcac accgattttg tttttacttt gcaaaaaact gcacctttgg
     3901 gcatgagctt tttactataa tccgcaagct ttcagcaata gatgatatta ttaaaacaat
     3961 aaatgattat tacatgcgta accccatgga tcaaaataaa cactatagag ttccgtaaag
     4021 gatacctcat gtgcatttta taaatacaaa gcaatattct tgacaatatt tgccatttcc
     4081 gatatgtatt agtattatac atttttattg tactatcgct gttttgaaag tatcatataa
     4141 attatgattg tatagtataa tgctgaatgt gaccataaaa aaaccatgaa agacattgaa
     4201 gaaatacttt cactattaca cgcaaataaa gattttttaa aagatcattt caaggtttgc
     4261 tctatagcaa tctatggttc gtattcacgg aatgatcaaa ctaaggatag ttatgtatta
     4321 tccgtcatat cttacactct atcataccgg cgagcttaaa aaaaggatcg atgccttaca
     4381 gaatattctt acctgttgta cgctgtgtcc tcatcgatgt agaattgacc ggcgcataaa
     4441 aacaggtaaa tgccgtaccg gtgttatggc gatagttgcc acataccatg ctcattttgg
     4501 tgaagaatca tgccttgttg gaagtcatgg ctccggtacc atcttctttg ccaattgcaa
     4561 cctttcgtgt atcttttgtc aaaactggga tatatcgcag atgcctaacg gtaaaccggt
     4621 tagccccgaa cagttagccg atgttatgat aagattacag caaaagggat gccacaatat
     4681 aaacctcgtc agcccaacac atgttgcggc acagattgtt gaagcattgc cgtatgcaat
     4741 tgaacgcggt cttactattc cgctggtata caacagtggc ggatacgatg cggtggaaac
     4801 attacaattg cttgacggca taatagacat ctatatgccc gattttaaat acatggatga
     4861 aacagttgca aaacatttaa gcggtgcacc tgattatcca caccacgcaa ctgctgcaat
     4921 cagggaaatg catcgtcagg ctggcgattt ggtgatagaa catgggatag caaagcgcgg
     4981 cttgcttata aggcacttgg tgatccctga gaatagtgct cgctcggatc tggtgtttaa
     5041 ggaaattgca aaactttcaa ttgataccta tataaatatt atggctcagt accaccctgc
     5101 atacagagca cgggaacacc ctgcactggg aagatacata acactggaag aatttaatca
     5161 ggcattgcaa tgggctaaag ctgcaggttt atggagattt gaccgataga atttatgatt
     5221 gacttcatta caattcataa ttattaatga aacattatgc tacattcatc gtcaaaactt
     5281 attaatcttt tcgatctggt caatgatgct attattatta ttgataaaaa tcacaatgtt
     5341 atttttaata atgaaaaagg acaaattttt gttaaatcag atggcaaaac tctactttta
     5401 gagaatgaaa cgataattaa cctggttaat acactacaac ctgatgctga agttattggt
     5461 gaacacataa gtttaaaagt aaaaaaaaga atccatcact atacgctcaa tgcaattgca
     5521 attaatgatg acaattcttt cttaattgca tttgttataa ccgacaccac ccgtttgcat
     5581 aaattgcacc aacaacagca gcaactcatc agtcagatac gtaaaaacta cttagatagg
     5641 tttgaatcgc ttaaacagct ggcagacagc atcgcccatg aagtaaggaa ccctttagtt
     5701 tctattggcg ggtatgccaa cttgcttata cgaaaatgtg atgaaggcat tgatcataat
     5761 gaagccaaaa aatatttaca atacattgta gacaatgctg aacgccttaa ctatttaaca
     5821 caacagattg aggaaatggg agatttaaag cgagttaatt tagcagcata tagtattaca
     5881 aaatttatgc aggaacagga gtcaaccttt atagctgctg ccagtcagtt taacaaaaaa
     5941 ttacaattaa atatagaatc cgaaaaaaat tataccatat atattgatta tgacaaatta
     6001 cgctttgcta ttacccagat tattgaattt gttgctgagc atacaaaagc agaaaatata
     6061 gtgctccatt gctatgggac atcctatgag ttgatcattc acctgcaatg tataaccgat
     6121 ataatagccg aagaagacct gcactatctt tttgatcctt tttatacaac acgctccaat
     6181 gtagaaacat ttaacctctg cattgcccag cgtatcatca tgctccatgg cggtattatc
     6241 acctcaagcc tgactcgtaa aacccttcat tttaaaatag cattgcctca ggataaacgg
     6301 ttaactcatg acaacggcaa gtgattcatc aattgctgtt actgttgtat cgatgtcctc
     6361 ttttgtatga gctatcgata aaaacccaac ttcataccct gatggagaaa ggtatatccc
     6421 ccgctccagc atctccctgt gaaattgagc aaactgctgc atgttgcatt cctttaccat
     6481 atcaacgctg gtaatgttgt cggcattagt aaagtataaa gcaaatattg atgcaaacct
     6541 gacaaaaaga aaacgcccgc caaagcgctt aacaacaggt tgcattgcat cctcaaagta
     6601 ttgacctaac tgctcaagtt tttgatatgt agacggaaca agcttttgaa gtgttgctat
     6661 accagcagcc attgctattg ggttgcctga taatgtacct gcctgatata ccggaccatc
     6721 aggtgcaacc tttgacatta tctcatgcga agcgccatac acacccacag gcaaaccgcc
     6781 acctgcaatc ttgcccagcg ttgtaatatc agccgtaaca ccaaagcgtt cctgagcgcc
     6841 accccgtgcc agacgaaatc ctgtaataac ctcatcaaag atcagcagta catgatattg
     6901 tgtacataga tcccgtacct tttgtaaata atcatcttcc ggcaaaatta atccataatt
     6961 gcagcaaaca ggctctataa tgcaacatgc aatatcatca ttgtgcgata gtacctggtg
     7021 aagggcatcc acgtcattat agggaataac tattgtatcc tttgcattgc cctttgttac
     7081 gcctgccgaa tcaggcatac caaatgttgc aagacctgaa ccggcagcaa ccagaagaaa
     7141 atcagcatga ccgtggtaac atccatcaaa ttttattatt ttactgttac cggtaaatcc
     7201 ccgtgcaagg cgtattgcac tcatggttgc ctcagtgcct gaattaacaa aacgaacctt
     7261 ttgcataccc ggaaacgaat ccacaacaag cctggcaatg tcaatctctg ctgcgtgggg
     7321 agcaccaaag cttgttccgt tttcggctat ttgctggatt gcctttacca catcattgtc
     7381 agcatgacct aaaataagcg gaccccatga catgcagtaa tcaataaatt cattgccatc
     7441 ttcatcaatg atgtgcgagc ccttccctaa cttaaagtac aggggatgtc ctcccaccga
     7501 tctaaatgca cgcaccggtg aattaacacc tccggggagg tatttacatg cctgtgcata
     7561 taattctttt gattttgatg taatcaatgc catgcaatcc tcctaatcaa accacctgtt
     7621 ttttaaaata tcacaggtat gatacgaaat taccatatca gctccggctc tgaatattga
     7681 tgtcataatt tcacgcacaa gctttgcctc atcgccaaat cccagtgtac ctgcagcttt
     7741 caccatggaa tactcaccac taacattata tgcacacacc ggcaattcta cagactgttt
     7801 aaccctgtaa ataatatcta aatatgataa cgcaggctta accataacga tatcagcacc
     7861 ctcgtcaata tcaagattaa cctctttcaa tgcttcatgt gcattgcgat agtccatttg
     7921 atagcttcgt ctgtcaccaa attgtggtgc gctacctgct gcatccctga acggaccata
     7981 aaaagctgaa gcatattttg ctgcatacga tagtatgggt atagttataa aaccgttatc
     8041 atcaagttcc tgacgaatag tttcaaccct gccatccatc atatccgaag gagcaataat
     8101 atcagcacca gcccttgcat gcgatacggc agtttttgct aaaagctcaa gggtactatc
     8161 gttatctatc tggtttcctt ttaccatacc acaatgccca tgactggtgt attcacacaa
     8221 acacacatcg gtgataatga gcaattccgg aaactgtttt ttaatttccc tgcaagcctt
     8281 ctggacaatc ccattttcat catacgcacc ggaggctttt tcatctttat catacggtat
     8341 tccaaacagg ataatggctc tgacccctaa ttttacatca taatcaagct gttttaaaag
     8401 attatcaata gatagccgct gtatacccgg catagactct atggcttgtg taatacccgc
     8461 cccttctata acaaaatagg gcataataag tttgttataa tcaggttttg tttcagcaat
     8521 taaatcacgt attatttcat tttcccgcag ccgacgaggt ctgtaatagg gatgcatcat
     8581 gtgcctcctt tcattcacat tcttaattat tttatttaat gatattacat ctgctctcag
     8641 taatgcacta atctacatta taattgaata tattcataaa taatcaaagt attttcaata
     8701 ataagtatgg ataataaaaa ttttagtaaa gtatattttt atgtaatttc attattttta
     8761 taaaaagaag tgatcatttt tttgtctgag tactcagaca tataggctct gatcccattt
     8821 tatattgtct tgatgaggtt aaaagaaagt agtggaggca cggatgccgt agcatgatat
     8881 ggctcgtgcg aggacaagga caggatgtcg tccgcagcac gaaggtcaaa acacactata
     8941 tacagtaaat aaggcacacc gccgtttgag gtgcgcttta ggggttggta agggggtatt
     9001 tagcgcataa atatcccctt acgaagtgat aaaactattg tcgatttgca aatgaaagta
     9061 tgagaaaaaa tatgaagatg tatcacatat ttccctttgg aaaaattata gtttgtcatt
     9121 ctgtacgaag tgaacccccc ataatccccc cctttgaacg aaagggtgga aatattcgat
     9181 tactggcagc tgctctatga tagtgttaac cgatgtcatt ttgaaacgaa ggcgaagaat
     9241 ctcttatttg cataaaaaat atagattctt cagtcgctga tgctccttta gaatgacaga
     9301 atagcagtat gctctgaccc cattatattt ttttacaaat ttataaaaaa atgcaccatt
     9361 tcttttcatt ttttttcatc tccggcgttt tatagagtag aaacttcaat tattataaca
     9421 acacggagga atacccatgg cacggaaatt gcgcctacaa ctgcccgagc atacctatca
     9481 catcatgagc cgctgtatcg agtggcggga
//
LOCUS       DAOSOX010000088         9271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_7389_length_9271_cov_9.100477, whole genome shotgun sequence.
ACCESSION   DAOSOX010000088 DAOSOX010000000
VERSION     DAOSOX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7389_length_9271_cov_9.100477"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..2652)
                     /gene="infB"
                     /locus_tag="PLX22_07490"
     CDS             complement(172..2652)
                     /gene="infB"
                     /locus_tag="PLX22_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT19781.1"
                     /translation="MKAIDVATKNHVSTDDVIKICKELGIPCDNEQSDLHNDDVFLIE
                     KKIQIIKEHRAQEAKKLIQQAELKKKIKLKRKVHVAKELKKETDGKGETVIHEGTDAR
                     KKAEVKTAEPSKQGRDEHKDGQRKNEKFERKDRQGKPFNKFYKKDGKKDDQKLKHGGL
                     EQKPEKPSAQPLHDKKDVAVIDKEVVDLQKRQKDKGKEKEKEKDKDKRLLKKEKAEFD
                     EKLSLLKRKKEALPREAVTPREIEITETITVGDLAKKMNIKASEVIAKLMSMGVMATI
                     NQVIDADTASILAGEYGTEVKVVSLYEETVIRAEEEDRPEDYVLRPPIVTVMGHVDHG
                     KTKLLDAIRTTNVVDTEHGGITQHIGAYRVKVGEKYVTFLDTPGHEAFTTMRARGASV
                     TDIVVLVVAADDGVMPQTIEAINHAKAANVPIIVAINKIDLPEANPGRVKQELTTYEL
                     IPEEWGGTTLFAEISAKQKININELLDLILIQAEMLELKANPALKAKGVVIESKLDPG
                     RGPIATVLVQNGTLRVGDPFVVGLYSGRVRAMFADTGEQVYEATPSMPVEVLGISGVP
                     SAGDPFQVVESEKYARQIAQKRQELVRLESAQKVKKVTLEDLNAMIKEGEIKELKIVI
                     KADVDGSVQALRDSLQKLSTNDIRVKVIHAATGGINESDVMLASASNAIIIGYQVRPS
                     SKVAEIADKENVSIQYYNIIYDAIADVKAAMEGMLSPEMKEEITSTGEIKQTFKISKV
                     GTIAGAVVLTGRLKRSDKIRLLRDSVVVFDGKLNSLKRFKNDAGEVEAGQECGFTLEN
                     FNDIKEGDTFESYQIIEIKKKLGDNSRK"
     gene            complement(2694..4016)
                     /gene="nusA"
                     /locus_tag="PLX22_07495"
     CDS             complement(2694..4016)
                     /gene="nusA"
                     /locus_tag="PLX22_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT19782.1"
                     /translation="MSGNFFEALHQIATEKGITREMIEEIVQSAMVSAYKKQYGITRP
                     VDIAFDRDKNTVKIISRKLVVRLPKNYAEEISLEDAQKIKPDAKFGDEVVVEENPLET
                     FGRIAAQTAKQVILQKIKEAEKNIVYDEYKDKVNELINGYVQRKTKEALYIDLGKTEG
                     IIPAREQSPLEHYKIGDRVKAIVLTVQKNSKGPSVVLSRAHPKFVEKLFEMEIPEIYD
                     DIVKIVSIVREAGLRTKVAVTSDRDDIDSVGACVGMKGVRIQSIVRELEGEKVDVIEY
                     SPNKKVMATNALTPAKVKEIIETRDGSVIAVVDNDQYRLAIGKNGHNARLAVKLCGFN
                     IDIKTEDQYREFLSSSESRALVEQLFATTQDGETPLEELPGLDARTITLLETGGIFSV
                     EDLVEKSVEDLMNIDGIGEKTAQKIINIIQEYVDFEEDDYEDEQEDKE"
     assembly_gap    4178..4277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4448..7531
                     /locus_tag="PLX22_07500"
     CDS             4448..7531
                     /locus_tag="PLX22_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19783.1"
                     /translation="MERDSLQSIKLELTKSLSFTHYVRICLHPVIASSHSQIAYALLC
                     SELQAEPAIRLSCIYTIVHYNIKGFTQHFHALLGQSITDEEIIAICSYLKVFGNVDST
                     AVLNNYVMHHYADESHHNAVIECLDTIRIVHQQDITLLTTLKTILNDTSAHHIIRYYA
                     LRALALYHDIKVLQSFLNQDESTLTGILDAIAFIGQDIINTNKEKDSTPGDSNLVIEL
                     RVFLSKLLPQFSEYSLKTKIACIHALIMCKHRETNEYLMKILTSDNESEKEHLLILLQ
                     HAITQLRDPEPFIRILISLGVVTPHHNKIIIENVLTFFTALQTDRTSTILKDKMFNYF
                     TVSLDSFFELYRKNYMISEVEEKNLPEIVQKIRNFIVSELTPQLLNMIVHYLKYNNNS
                     EIATILTPITNHIQQIDSSSREGFLLLIEMLYDSDPKSREISASRLETIDFEKRFLRD
                     RIVRLCAIIAKLNIQSAAPLLVKIYNYCKKYRDDVIFDACINTLAAMQYPYMLGELEL
                     MLLSGDAKEQLFALNYFIHYKDEHASAILFEVLKNTSTGNEVVQIALNLLTTMQIAQF
                     KGSTEILKNIVQTHASNSVKKAAILCIGHCGEGKDIEWLIAQFNESNDTAIKESILQA
                     ISSIIPRLRDFNKRPIIHFLLDCLKESGIRVRMYACAVLVQLNNKDVENHIKEMLIIK
                     NKEIQSEILFIFQNSKLPEFSFFLLSLLKEEYAIVSEIIHFLHNIPSEISDDIATFIG
                     NLYKKNGVDLSSVTPEAITIKHGKIEWLKKYYIMKITIPQESNPETLEETVSKRDTLH
                     SLIINHCKNNAVHIKYILPHSIVAYSENAMNIANACIAMEYDIKHFNQHAFNPFNAII
                     QCYHALVMEFGQYFIVLTMDRYLHNTLYNYGIIDDGLYTSIQNDYACNSLPYILASPA
                     YIPIYYIANKKNAPFEAQKALDEIIVMEKTKKEKEQQLSEEIRKRKLHIQSQGTAEYI
                     ETLEKVNAILRSEINEINKYVQKRSADRELNTQLLKMLENMQKKIFLEISSFMMK"
     gene            complement(7568..8419)
                     /locus_tag="PLX22_07505"
     CDS             complement(7568..8419)
                     /locus_tag="PLX22_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19784.1"
                     /translation="MLSVSLTIGLLLVAVFNNSPAQDELAVTIEQKPQYTIYEREGIL
                     YCTGNTEAAVCNNKGADELAANNINTAIAIFQEGLVHAPLFYPFLYNIGIAYMRNRQF
                     DEARVYLEKAKHIVPENPRIYILIGELYAFTGREGDALVLFKQALNIHKKELDAIIKI
                     GTIYIERNQLELALRYFNSALDINPKYPDAGIGVAKVFFKKGEYYKALIRLKSIDITQ
                     GNYDKAYHYYYAESSYKLKDYKTAYEQYKQLLVYKDDIFFLTHSYALIEHRMNLARQF
                     AELENVQ"
     gene            complement(8401..>9271)
                     /locus_tag="PLX22_07510"
     CDS             complement(8401..>9271)
                     /locus_tag="PLX22_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT19785.1"
                     /translation="VDRVSLSIPEGNTYGLVGESGCGKTTLARALLRLIEPDSGEIMI
                     RNTPVHSLQKKELRQFRKNMQIVFQDPYGSLNPRMTVEKIIAEALKIHCQMTKKDIAS
                     RVSELLAMVGLDKNFASRYPHELSGGQRQRIGIARAIALSPSFIVLDEPISALDVSIQ
                     GQILNLLLDIQEQCGMSYLFVAHNLAVVKHISTMVGVMYLGKLVEENTASMLYRKPLH
                     PYTRSLLSSIPDVKPVKHLFSVVKGEIPSPENPPAGCHFHPRCPWVMPVCKEAYPAIV
                     TVNGAKVACFLYH"
BASE COUNT         2720 a         2002 c         1485 g         2964 t
ORIGIN      
        1 cccaggattg aaatatatac atcagcatat gcataatctt tactcatctt tacccgtgtt
       61 accgttacaa aaccaatcct gggatctttg agttcggtta caataagggt gccaattaat
      121 ttctgtattt gtgcttctaa cttttgtttt ctgtacccca tggttatgcc actatttacg
      181 tgagttgtct cccaatttct tttttatctc tattatttga tatgattcaa aggtatcacc
      241 ttctttgatg tcgttaaaat tttccagtgt aaaaccgcat tcctgtcctg cttcaacctc
      301 acctgcatca tttttaaagc gcttcagcga attgagcttc ccatcaaata ccactacact
      361 atctcgtaac agccgtattt tatcgcttcg tttcaagcgc ccggtgagta caacagcgcc
      421 ggcaatagtt ccaaccttgc ttatcttaaa tgtttgttta atctcaccgg tggatgttat
      481 ctcttctttc atctcaggag atagcatacc ctccatcgca gccttcacat cagcaatggc
      541 atcgtagata atattgtaat attggatcga gacattttcc ttgtcagcta tctctgctac
      601 ttttgatgat ggacgcacct gatagccaat aataattgca tttgaagcgg atgccagcat
      661 aacatccgat tcattgatac caccagttgc cgcatgtata acctttaccc gtatgtcgtt
      721 ggtagaaagt ttctgtaaag agtcgcgcaa tgcctgtact gagccatcaa catcagcctt
      781 aataacaatc tttagttcct taatctcacc ttctttaatc attgcattaa ggtcttccag
      841 agtaaccttc tttaccttct gagcgctttc cagacgtaca agctcctgcc ttttctgagc
      901 tatctgtcgg gcatattttt ccgattctac tacctgaaat gggtcgccag ccgaagggac
      961 accgcttatt cccagcacct caacaggcat ggacggagtg gcttcataaa cctgttcgcc
     1021 agtatcagca aacatggctc gcaccctgcc cgaataaagc ccaaccacaa acgggtcgcc
     1081 aacccttaac gtaccatttt gaaccagcac cgtagcgatg ggtcctctgc ctggatcaag
     1141 tttagattca ataaccaccc cttttgcctt caatgctgga tttgccttta attccagcat
     1201 ctcagcctga atcaaaatta aatctaaaag ctcattgata tttatttttt gctttgcact
     1261 aatttcagca aaaagcgttg ttccacccca ctcctcaggt atcagctcat aggtggttaa
     1321 ttcctgctta acacgacccg ggtttgcttc aggtaaatca attttattta tagcaacgat
     1381 gattggtaca tttgctgctt ttgcgtggtt tattgcttct attgtttgcg gcataacacc
     1441 atcatcagca gcaacaacta aaacaactat atcggtaacg cttgcaccac gtgcacgcat
     1501 tgtggtaaac gcctcatggc cgggtgtatc taaaaacgta acatattttt cgccaacctt
     1561 tacccgataa gcaccaatgt gctgagtaat accaccatgt tcagtatcca caacattggt
     1621 tgtcctgatt gcatcaagaa gttttgtttt accatggtca acatgaccca taacagtaac
     1681 aatgggtgga cgcaaaacat aatcttctgg cctatcttcc tcctctgctc gtataacagt
     1741 ttcttcatac agcgaaacaa ccttaacctc ggtgccatat tcaccagcta aaattgaagc
     1801 agtatcggca tcaatgacct gattgatagt tgccatcaca cccatcgaca taagcttggc
     1861 tataacctcg cttgccttta tattcatctt tttggcaagg tcacccacgg taatcgtttc
     1921 tgtaatttct atctctcttg gagtaacagc ttcccttggc aaagcttcct ttttcctttt
     1981 caataatgac agtttttcgt caaactctgc tttctccttt ttcagaagac gtttatcttt
     2041 atctttttct ttttcctttt cttttccttt atctttttgc cgcttctgca aatcaacaac
     2101 ttctttatca ataacagcaa catccttttt atcatgtaag ggttgcgctg atggtttttc
     2161 cggcttttgt tctagtccgc catgttttag tttttgatcg tctttttttc catctttttt
     2221 ataaaatttg ttaaaaggct taccctgcct gtcttttctc tcaaattttt cattttttct
     2281 ttgcccatcc ttatgttcat cccttccttg tttagacggc tcagcagttt ttacctctgc
     2341 tttttttctg gcatcagttc cctcatggat taccgtttct cctttaccgt ctgtttcttt
     2401 ctttagttct tttgcaacat gtaccttccg ttttaattta atcttctttt ttaactcagc
     2461 ctgttgaatc agctttttgg cttcctgagc ccgatgctcc ttaataatct gaatcttctt
     2521 ttcaataaga aatacatcat cgttgtgcaa atcgctttgt tcattgtcgc aagggatgcc
     2581 taattccttg cagattttta taacatcatc cgttgaaaca tgattttttg ttgcaacatc
     2641 aattgctttc attaacaaat ccctattcat actaaaatta ttgacaggag catctattcc
     2701 ttatcttcct gttcatcttc ataatcatct tcttcaaagt caacatattc ttgaattata
     2761 ttaataatct tctgagctgt tttttctcct ataccatcaa tgttcataag gtcctcaacg
     2821 gatttctcaa ccagatcctc aaccgagaag ataccgcctg tttcaagcaa tgtgatagta
     2881 cgagcatcaa gccctggcaa ttcctctaat ggtgtttcac catcctgtgt tgtggcaaaa
     2941 agttgctcaa ccaatgcccg tgattcactt gaacttaaga attcacggta ctgatcctcg
     3001 gtcttgatat cgatattaaa tccacacaat tttaccgcta aacgcgcatt atgtccattt
     3061 ttacctattg ccagcctgta ttgatcatta tctacaaccg ctataacaga accatcacgt
     3121 gtttctataa tttcctttac ttttgccggt gtcagcgcat ttgttgccat aactttttta
     3181 ttgggagaat attctataac atctaccttt tcaccttcaa gttcgcgcac aatagactgt
     3241 atcctgactc ccttcatacc cacacatgca ccaacgctgt caatatcatc cctgtcactg
     3301 gtgactgcaa ctttggtacg caagccggct tcgcgtacaa tggaaacaat cttaactatg
     3361 tcatcgtaaa tttcgggtat ctccatttca aaaagttttt ccacaaattt tgggtgagca
     3421 cgcgataaaa caacacttgg gcctttactg tttttttgca cagtaaggac tatcgcctta
     3481 accctgtctc caatcttata gtgctcaagg ggcgattgct cacgtgcagg aataataccc
     3541 tctgttttcc ctaaatcaat ataaagcgct tctttggttt tacgctgtac atacccgttg
     3601 ataagctcgt ttaccttatc tttatattca tcgtatacaa tatttttttc tgcttccttt
     3661 atcttttgta atatcacctg cttggcagtc tgagcagcaa tgcgtccaaa cgtttcaaga
     3721 ggattttctt ccaccacaac ttcatcgcca aactttgcat ccggcttaat cttttgtgcg
     3781 tcctccagtg atatttcttc agcatagttt ttgggaagcc gtacaaccag tttacgtgaa
     3841 attatcttca cggtattttt gtctctatca aaggcaatat caactggcct ggtaatgcca
     3901 tattgtttct tataagccga taccattgca gattgcacta tctcctcaat catttcacga
     3961 gttatacctt tttcagttgc aatctggtgt agcgcttcaa aaaaatttcc actcatagca
     4021 atcaccttta ataatctaaa tttgctttaa ctatctgcat atagggaaca acaataatct
     4081 tttgtccttc atcaatagta actatcgcat tgtcaaccct ctcaagatgc cctttgaatg
     4141 ttttctttac atcatcctcc aggtataccc ctttcacnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnaaa gcagaaaacc aactacctta attaatcaat atattactat
     4321 atgcttacaa tttcaagctt ttttttatct ttacttttat caaaagtaat attctaattt
     4381 acagtattac aaacaatact ttctattgtt atataaactg tataaaccct ttataaaaag
     4441 gtgcgctatg gaacgtgata gtttacaatc aattaaatta gaattaacaa aaagcctgtc
     4501 atttacgcac tatgtaagga tatgcctgca tcctgttatt gcatcatcgc atagccagat
     4561 agcatacgct ctgctatgct cagagcttca ggctgaaccg gcaatacggt taagctgcat
     4621 ttatactata gtccattata acattaaagg ttttacgcag cattttcatg ctcttttagg
     4681 acaatccatt accgatgaag agataattgc catttgttca tatctgaaag tgtttggtaa
     4741 cgtcgattca actgcagtcc ttaacaacta tgtgatgcac cattatgctg atgaatctca
     4801 tcacaatgct gtcatagaat gtctggacac tatccgaata gtgcatcagc aggacatcac
     4861 actccttaca acactgaaaa caattctaaa tgatacttct gcacatcata tcatacggta
     4921 ttatgcatta cgtgcacttg cactttacca tgatattaaa gtattgcagt ccttcttaaa
     4981 tcaggatgaa agtacgctca caggaatact ggatgccatt gcttttatag gtcaggacat
     5041 tataaatacc aataaagaaa aagattctac tccaggcgat agtaaccttg tcattgaact
     5101 cagagtgttt ttatcaaagc ttttaccaca atttagcgaa tattctctga aaacaaagat
     5161 agcatgcata catgcactta ttatgtgcaa gcaccgggaa actaacgaat acctcatgaa
     5221 gatactcaca tcagacaatg aaagtgaaaa agaacatctt ctgatacttc tgcaacatgc
     5281 tattacccaa ttacgggatc ctgaaccatt catacggata ctcatatcac tgggggttgt
     5341 tactccgcac cataataaaa ttataataga aaatgtttta acattcttta cagcacttca
     5401 aaccgacaga acaagtacca ttttaaaaga caaaatgttc aattacttta ctgtttcact
     5461 ggattcgttt tttgagctat accgtaaaaa ttacatgatt tcagaagtag aagaaaaaaa
     5521 tctccctgaa attgttcaaa aaataagaaa ttttattgta tccgaattaa caccacaatt
     5581 attgaatatg attgttcatt atctgaaata taataacaat agtgaaattg ccacaatact
     5641 tactcccatc accaatcaca tccagcaaat tgatagttcc agcagagagg gatttttgct
     5701 tcttattgaa atgctttacg atagcgatcc aaaaagcaga gagataagcg catcacgatt
     5761 ggaaaccatt gattttgaaa aacgatttct gcgtgatagg atagtcaggc tatgtgctat
     5821 cattgcaaaa ctcaatatac aatctgccgc ccctttgctg gtgaaaattt ataattattg
     5881 taaaaaatac agggatgacg ttatctttga tgcatgcata aatacattgg cagcaatgca
     5941 atacccctac atgctgggcg agctggagct tatgctttta tccggagatg caaaagaaca
     6001 gctatttgca ctcaactatt ttatccacta caaagatgag catgcctcgg caatcctttt
     6061 tgaagtttta aaaaatacgt ccactggtaa tgaggttgta cagattgccc tcaatctgtt
     6121 aacaaccatg caaatagcac aattcaaagg cagcacagaa atattgaaga atatagtaca
     6181 aactcatgcc agcaatagtg taaaaaaagc agcgattctt tgtattgggc attgcggaga
     6241 gggaaaagat atagaatggc ttattgcaca atttaatgaa tcaaatgata ctgcaataaa
     6301 agaatcaatt ctacaggcaa tcagcagtat cataccccgc ctcagagatt ttaataaacg
     6361 gccaataata cattttttgc tggattgctt aaaagaatcg gggatacggg tgcggatgta
     6421 tgcctgcgcg gtgttggttc agcttaataa taaagacgtt gaaaaccata tcaaagagat
     6481 gcttatcatt aaaaacaagg aaatccaatc agaaatatta tttatttttc aaaatagtaa
     6541 acttcctgaa ttttcgtttt ttttgctttc tcttttaaaa gaggaatatg caatagtatc
     6601 agaaataata cattttttac acaatatccc gtcagaaata agcgatgata ttgccacttt
     6661 cattggtaat ttatacaaaa aaaatggcgt ggacttgtca tcagtaactc cagaagctat
     6721 caccattaaa cacggtaaaa ttgaatggtt aaaaaaatat tatatcatga aaataaccat
     6781 cccgcaggaa tctaatcctg aaacacttga agaaacagta agcaaacgtg acaccctgca
     6841 ttcacttatt attaatcatt gcaaaaacaa tgccgtgcat attaaatata tactgcctca
     6901 ttcaattgtt gcatattcag agaatgcaat gaatattgcc aatgcctgta tagcaatgga
     6961 atatgatatt aagcatttta atcagcatgc attcaatcca tttaatgcaa ttatacagtg
     7021 ttaccatgct ctggttatgg agtttgggca gtattttata gtccttacca tggataggta
     7081 tctccataat acattatata actatggcat catagatgat ggtttatata catcaattca
     7141 aaatgattat gcatgcaata gtttgcccta catccttgca tctccagcgt atataccaat
     7201 ttattacatt gccaataaaa agaatgcacc atttgaagca caaaaagccc ttgatgaaat
     7261 tattgtcatg gagaagacaa aaaaagaaaa agagcaacag ttaagcgaag agatacgaaa
     7321 acgaaagctt catatacaat cgcagggaac tgccgaatac attgaaacac ttgaaaaggt
     7381 taatgcaata ctcagaagtg aaattaacga gattaacaaa tatgttcaaa aacgaagcgc
     7441 cgatagagag cttaacacac agttgttaaa aatgcttgaa aatatgcaga aaaaaatatt
     7501 tttagagatt tccagtttta tgatgaaata atgatggaaa tacttttgtg ggctaagcct
     7561 gagtatgcta ttgtacattt tcaagttcag caaactgtct ggcaagattc atcctgtgtt
     7621 caattaaagc atatgaatgg gtaagaaaaa atatatcatc tttatacact aatagctgct
     7681 tgtattgctc atatgctgtc ttataatctt ttaatttata tgagctttca gcataatagt
     7741 agtgataggc tttatcatag tttccttgtg tgatatcaat agattttaat cgtatcagcg
     7801 ccttataata ttcccctttt ttaaaaaaaa cttttgcaac accaatacca gcatcagggt
     7861 attttggatt aatatccagt gcgctgttaa aataccgcaa tgctaactcc agttgattgc
     7921 gctcaatata gatagtacca atcttaataa tggcatccag ctctttctta tgaatgttta
     7981 atgcctgttt aaacaaaaca agagcatccc cctccctgcc ggtaaaagca tagagctctc
     8041 ctatcagaat atatatcctg ggattttccg gaacaatatg ttttgctttt tctaaataaa
     8101 cccgtgcttc atcaaactgc ctattgcgca tataggcaat cccaatatta tacagaaacg
     8161 gataaaataa aggagcatgg actaaccctt cctgaaatat cgctattgca gtgtttatat
     8221 tattagcggc aagctcatca gcgcctttat tgttacaaac tgcagcttcg gtgttgcccg
     8281 tacagtataa aattccttcc ctctcataaa tggtgtattg cggtttttgt tcgatagtta
     8341 ctgccaattc atcctgtgcc ggggaattat tgaaaacagc tacaagcaac aaccctattg
     8401 tcagtgatac agaaagcatg caacctttgc tccattaaca gtaactatcg ccggatatgc
     8461 ttctttacat accggcataa cccagggaca gcgtgggtgg aagtgacatc ctgctggagg
     8521 attttccgga gaggggatct cccccttcac aaccgagaac aaatgcttta ccggtttaac
     8581 atccgggatg gacgacaata aactgcgcgt atacggatgg agaggctttc tatagagcat
     8641 gctggcagta ttttcttcaa ccaatttgcc cagatacatt accccaacca tagtgctgat
     8701 atgtttaaca accgctaaat tgtgtgcaac aaaaagatac gacatcccgc attgttcctg
     8761 aatatcaagc aataaattca gaatctgtcc ctgtattgat acatcaagcg ctgatattgg
     8821 ctcatccagt acaataaatg atgggcttaa tgcaatcgcc cgcgcaatcc ctatccgctg
     8881 tcgctgtccg ccgctgagtt catggggata gcgtgatgca aagtttttat caagtccaac
     8941 cattgccaat aattcagata ccctgctggc aatatctttt tttgtcatct ggcaatgaat
     9001 cttcagcgct tcagcaatta tcttttcaac agtcatccga ggattaagcg aaccataggg
     9061 atcctgaaaa actatttgca tattttttct aaactgtcgt aattcttttt tttgcagtga
     9121 atgaacaggg gtattccgta tcattatttc tccgctatca ggttcaatga gcctgagcaa
     9181 tgcacgagct aatgttgttt taccgcatcc actttcgcca accagtccgt aggtattccc
     9241 ttcaggtatg gataagctta ccctgtcaac t
//
LOCUS       DAOSOX010000089         9101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_7590_length_9101_cov_8.789631, whole genome shotgun sequence.
ACCESSION   DAOSOX010000089 DAOSOX010000000
VERSION     DAOSOX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_9101_cov_8.789631"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..430
                     /locus_tag="PLX22_07515"
     CDS             68..430
                     /locus_tag="PLX22_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19786.1"
                     /translation="MGTIAKSSGEINGIIAIIINDISDRINLLSLNAAIETARAGDHR
                     RGFAVVAGEVSKLADQTAVSIKTIGSLIINNDREIKTGMQNVSQAIGTINSIIKDIDV
                     IVSHISAISDTFKAGRGK"
     gene            455..661
                     /locus_tag="PLX22_07520"
     CDS             455..661
                     /locus_tag="PLX22_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19787.1"
                     /translation="MSLLSEQIKIAMDEQKSAISEIAKTVSSINELAQENTAKGLDIT
                     ETSRALVGKVQSINKEIDEFSSGT"
     gene            889..1518
                     /locus_tag="PLX22_07525"
     CDS             889..1518
                     /locus_tag="PLX22_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOT19788.1"
                     /translation="MPFYKFIPHEIKFFDLFDRQADKMVEAVICFKEFAEKGIFDDSC
                     IERMRLLEHQSDSIAHDIIDSLNRTFITPFDREDIHSLTNKLDDVVDLIYAISKRMRL
                     YKLKGKNADVQEFSRLIMQSVQSLSLAIKGLRNHRQPQIIQQACIEINKIENISDQLR
                     DVVIQRLFKSKKDPIAIIKWKEIYELLETVLDVCEDVSNIVVSILVKQG"
     gene            1525..2526
                     /locus_tag="PLX22_07530"
     CDS             1525..2526
                     /locus_tag="PLX22_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOT19789.1"
                     /translation="MTIYIFFLIALALLFDFLNGFHDAANSIATVVSTRVLSPRYAVL
                     WAAFFNFVAFLFFGLHVAGTIGNGIIDVSIMDNTVILATLTGACMWNIITWYLGLPTS
                     SSHALIGGLIGAALMKVGTKALVLDGIIKTVIFIFLSPIVGFGLGLVFGTAVYWIFRH
                     SHPAKVDKIFRKGQLISAAFYSLGHGGNDAQKTMGIIAGLLFSNGLMQVAEGQRFQDA
                     IPLWVVLACHSAIALGTMFGGWRIVKTMGQRVVKLRPVDGFCAELGAAFNLYVITSYF
                     GIPVSTTHTITGAIMGVGTLRRFTAVRWGVAGQIVWSWILTIPLSALIAAITYSIVT"
     gene            complement(2546..4408)
                     /locus_tag="PLX22_07535"
     CDS             complement(2546..4408)
                     /locus_tag="PLX22_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT19790.1"
                     /translation="MQLIEIIKELEATNKNIPSLEIDSYRKHFSKPVFYAKNYNKEFP
                     DKFVTITGEEFLTLQYNIASALLAYGVEKDTIVGIFSRNNLYYAATVFAVMSLGAIVM
                     PIYPTILQDELEEILRDSGCRTIFVGDAAQFQKSFAILNKVQSPLRRLITYFDSKTKH
                     EQLVTFDELIQFGEEKAHYEEINRAIEKINSEDYAALMYTPGTSGDPRGVPLTHGNFI
                     SQHVVIPIFKFSDADIRLAHLPFSHVYGFAADLLISSFTGAPMAISTTLESEEIYRNM
                     IDIKPTVIATVPRMYEKLYINTIHTIRRFNPIKRFIYFSSINIGRGCYIAQTSGKTIP
                     KGAALFNKLLSPITKSIQKLMHMQQVRLLFSGGAPLPIDVSIFFGGIGLPIIEGYGLT
                     ETSPTVTINRIEKNKPGTVGYPIPGAEVKISDEGEILIKGPMVFKGYHKMTPEERLEV
                     FTASGYFRSGDIGTIDEDGYLRITGRLKDIIITSQGKNIVPLHIEKKFEAEPLINYIC
                     VVGDNRKYLSALIVPNFHLLRQYAREHGISYKTDEELVSHHQIQTLFKTRINEICQTV
                     APYEQIKKFTLLAHDFTVKTGELTPTFKFRRSQIHEKYKDLIDLMYPKSDTTRL"
     gene            4774..5994
                     /locus_tag="PLX22_07540"
     CDS             4774..5994
                     /locus_tag="PLX22_07540"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOT19791.1"
                     /translation="MKTANIPELIEEIRNGRMIILVDNEDRENEGDLVVAAQYCTPDK
                     INFMATHGKGLICIAMTQERLQELGLPLMVPENSDKFCTAFTVSVDAKEGTSTGISAY
                     DRAKTVHALIDPRTKPQDLNKPGHIFPLTARKGGVLVRAGHTEGSFDLTRLAGLYPAA
                     VMCEILNDDGTMARRPDLDAFAEKHNLKIGTIEDLIKFRQHKERLVHRVSEAHLPTEY
                     GDFKIIAYETEVDNATHVALVKGEVDGRENVLVRVHSECLTGDVFGSHRCDCGSQLHQ
                     ALRMIEKEGLGTLVYMRQEGRGIGLSNKLKAYHLQEQGYDTVEANIKLGFPADLRNYG
                     IGAQILVDLGLSTIRLMTNNPKKVIGLEGYGLTIVERVPIEIPPIKENYRYLKTKKDK
                     LGHLILQAQEDKKS"
     gene            5991..6899
                     /locus_tag="PLX22_07545"
     CDS             5991..6899
                     /locus_tag="PLX22_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT19792.1"
                     /translation="MKNGQAPTIVVVDDDEVIRYSLQKKLSRLGYNIISLSKAEDALF
                     LLKNDEREIDLIITDIKLRKMDGIELLRHVNVSENPVPTLIITGQGNIEDAIKALRYG
                     ALDFIRKPFDMNEVASVVRNILRRRQQHSLENTLGQYIVDDHRTLVIPIDETIADLVA
                     YELTKHLSYAKFCNRSTAENISMALLEALSNAMYHGNLEIPSEVREEHGVKGFHDEIQ
                     KRLQDSQYNQRKVTIRYSYTGEYVEYIIEDEGKGFDYKNVADPRDPENFLKNSGRGLF
                     IIQVHMDEVEWDNGGRRIRMRKYKTL"
     gene            6912..7946
                     /gene="mtnA"
                     /locus_tag="PLX22_07550"
     CDS             6912..7946
                     /gene="mtnA"
                     /locus_tag="PLX22_07550"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT19793.1"
                     /translation="MYYTIKWDDTAVSMLDQRMLPVQEIYRHYTNYHDVADAIRSMVI
                     RGAPAIGIAAAMGIALAAVKNKLSVHDEFKTEMVRVCDEFAQTRPTAVNLFWAISRMK
                     TIIEKATDIQHCKEALVDEAKKIHQEDIAINKTIGENGQKFLDDGMTVMTYCNAGALA
                     TGGYGTALGVIRSAIEHGKKIKVYACETRPYLQGARITAWELYKDGIETTLICDNMAG
                     YFMKKGEINCVIVGADRIAANGDTANKIGTYTMSLLAKAHAIPFYVAAPLSTLDTNTI
                     NGDSIIIEERSIDEVAYIQGVQIAPEGIFIKNPAFDITPADNITAIITEQGIVEHPAA
                     TGIAPLFSVR"
     gene            8186..8473
                     /locus_tag="PLX22_07555"
     CDS             8186..8473
                     /locus_tag="PLX22_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT19794.1"
                     /translation="MNKYYIYMITNRNNNVLFIGVTNDLKRRVFEHREKLVEGFSSKY
                     NCSKLVWYEQTNNIGSAILREKQMKKWKREYNNNIINEMNPEWNDLYETIL"
     gene            8549..8977
                     /locus_tag="PLX22_07560"
     CDS             8549..8977
                     /locus_tag="PLX22_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19795.1"
                     /translation="MRQAISIIPVILSMVLYGTIGVARDIPYTLEDRERLIRVETKLE
                     EGYKALHSRIDAVDKRIDAVDKRIDDVKGLLYVLIAAIFAQTVGVVGFVVWDRRTALS
                     PAINKTKEIEEKEDKIITALKQSAMKDKNIAEALRKAGML"
BASE COUNT         2925 a         1448 c         1969 g         2759 t
ORIGIN      
        1 aagaatgcag gatattttta aaaaagcaca atccggtgag gcatcacttg ctcttatgag
       61 tgagtaaatg ggaactatcg ccaaaagttc tggtgaaatc aacggaatca ttgcaataat
      121 aataaatgat atatcagaca gaattaattt actgtcgctc aatgctgcta ttgaaaccgc
      181 tcgtgctggg gatcatagaa gaggttttgc agttgttgca ggtgaagtat caaaattagc
      241 cgatcagaca gctgtaagta taaaaaccat aggatcgcta ataataaaca atgatcgtga
      301 aataaaaact gggatgcaaa acgtttctca ggcaataggg acaattaata gcataattaa
      361 agatatagat gttatcgttt cacatatcag tgcaatatct gatactttta aggcaggccg
      421 aggaaaataa taaagctgtg aaggtgggaa cgctgtgagt ttgctttcag agcagataaa
      481 aattgcaatg gatgaacaaa aatcagcaat tagcgaaata gcaaaaacgg tgagttctat
      541 caatgaatta gcacaggaga atactgcaaa aggattggat attaccgaaa cttccagagc
      601 acttgtcggc aaagtgcaat ctataaataa agagatagat gagttttcat ctggaacata
      661 ggtatctcac ggtatgatgg taaatgtaaa attttcattt tactgccccc ataatgtata
      721 ctattgatac atctgatatt agcaagattt ttgccggatt agatagcccg gtttttgatg
      781 tacccataaa ataactattg acaggagttg ttccctgata aattgtaact tttcaataat
      841 taatgattta agatacatat atttttagct atatacggag ataataatat gccgttttat
      901 aaatttatac cccatgaaat aaaatttttt gatttgtttg atagacaggc tgataaaatg
      961 gtagaagctg tcatatgctt taaggaattt gctgaaaaag gaatatttga cgatagttgt
     1021 attgaaagga tgcgtttgtt agagcatcaa agtgacagca tcgcacatga tattattgat
     1081 agccttaaca gaacttttat aacaccattt gatagggaag atatccactc attaaccaat
     1141 aagcttgatg atgtggttga tttaatttat gctatatcaa agcgtatgcg gctatataaa
     1201 ttaaaaggga aaaatgcaga tgttcaggag ttttccaggc tcataatgca atcagtgcaa
     1261 tcattatctc ttgctataaa aggtttgcga aatcacagac agccacaaat aattcagcaa
     1321 gcctgtattg aaataaataa aatagaaaat atcagtgatc agttgcgcga tgtggttata
     1381 cagaggttat ttaaaagtaa aaaggatcct attgcaatta tcaagtggaa agaaatatat
     1441 gaattattgg aaactgtatt agatgtctgt gaagatgttt ccaatattgt tgtttccatt
     1501 cttgttaaac aaggttaaat ctttatgact atctatatct tttttttgat tgcgctggca
     1561 ttgctgtttg atttccttaa tggtttccat gatgcagcca attcaatagc taccgtcgta
     1621 tctacaaggg tactatcacc ccgctatgct gtattgtggg cagccttttt taattttgtt
     1681 gcttttttat tttttggact gcatgttgct ggtaccatag gcaatggtat tattgatgtt
     1741 tctatcatgg ataatacagt aatactggca acactaaccg gtgcgtgtat gtggaatatc
     1801 ataacatggt atttagggtt gccaacaagt tcatcacatg ctcttattgg agggttgatt
     1861 ggcgctgcat tgatgaaagt tggcacgaaa gcattggtgc ttgatggtat tataaaaact
     1921 gttattttta tctttttatc gccgatagtt ggttttggat tagggcttgt ttttgggact
     1981 gcagtttact ggatattccg acactcacat ccagctaagg ttgataaaat attcagaaaa
     2041 ggacaattga tatcggcagc gttctatagc ctgggacatg gcggaaacga tgcacaaaaa
     2101 actatgggta ttatagcagg tttgctattt agcaatgggc tgatgcaggt tgctgaaggg
     2161 cagcgatttc aggatgctat tcccttatgg gttgtgcttg catgccatag tgcaattgca
     2221 ttgggcacca tgtttggtgg atggcgcatt gtaaaaacga tgggacagcg tgtggtaaag
     2281 ttacggccgg tagacggttt ttgtgctgaa ttgggagcag cttttaatct ttatgttata
     2341 acatcatatt ttggtatccc cgtgagtacc acgcatacca tcactggcgc aattatgggg
     2401 gttggaactt tacgccgatt tactgctgtt cggtggggcg ttgcaggaca gatagtatgg
     2461 tcatggatac tgacaattcc attatctgca ctcattgcgg caataacata cagtattgtt
     2521 acataatctc agttgcacta ttggtttata gccttgttgt atcacttttt ggatacatca
     2581 aatcaatcaa atctttatat ttttcatgta tctggctgcg tctgaattta aatgttggag
     2641 taagctctcc tgtttttaca gtaaagtcat gcgctaacag cgtaaatttt tttatctgtt
     2701 cataaggagc cactgtctgg catatttcat tgattctggt tttaaacaat gtttgtatct
     2761 ggtggtgtga tacaagctct tcatcagttt tatagcttat tccgtgttca cgggcatatt
     2821 gccgtaaaag atggaaatta gggactatca acgcagacaa atattttctg ttatcaccaa
     2881 caacacatat ataatttatt aaaggttcgg cttcaaattt cttttcaata tgcaaaggaa
     2941 ctatattctt accctgtgag gtgataatga tatctttgag ccttcctgtt attcgtaaat
     3001 agccatcctc atctattgtg cctatatcac cagagcggaa gtagccgctg gcggtaaaaa
     3061 cttccaatct ttcttcaggt gtcatcttgt gatatccctt gaaaaccatt ggccctttga
     3121 tgagtatttc accttcgtca gatattttaa cttctgcacc ggggatggga tatccaacag
     3181 taccgggctt gtttttttct atacggttga tagtaactgt tggagatgtt tcggttaagc
     3241 cgtatccctc aataatggga agtccaatcc caccaaagaa aatagataca tcaataggta
     3301 aaggagcacc acctgaaaaa agtagcctga cctgctgcat gtgcattaac ttttgtatac
     3361 ttttagtaat tggtgataat agtttattaa atagtgcagc acctttagga atagttttac
     3421 cagatgtttg tgcaatgtag catccccttc caatattgat agatgaaaaa taaataaatc
     3481 tttttattgg attgaaacgg cgaatagtat gaatggtatt tatatagagc ttttcataca
     3541 ttctggggac tgtagcaata accgttggtt ttatatcgat catgttacga taaatctctt
     3601 cactttcaag agtggttgag atggccattg gtgcaccggt aaatgagctt attaataaat
     3661 cggcagcaaa accataaaca tggctaaatg gcaggtgagc caaccgtata tctgcatcgg
     3721 aaaatttgaa tatagggatt acaacatgct gtgagataaa gttgccatga gtaagtggaa
     3781 cgccccttgg gtcacctgat gtacctggag tatacataag tgctgcataa tcttcggaat
     3841 tgattttttc tattgctcgg ttaatttctt cgtaatgagc tttttcttcc ccaaattgta
     3901 tcagttcatc aaaggttaca agctgctcat gctttgtttt agaatcaaag tatgttataa
     3961 gtcttcgcag gggagattgt actttattta atatagcaaa gcttttttga aactgtgcag
     4021 catcgccaac aaagattgtt ctacatccag aatcacgcag tatttcttcc agttcatcct
     4081 gtaagatggt aggataaatt ggcataacta tcgccccaag ggacatgacg gcaaaaactg
     4141 ttgccgcata atataaatta ttgcggctaa atatacctac tatggtatct ttttcaactc
     4201 cataggctaa tagtgctgaa gctatattat actgaagggt taaaaactct tcccctgtta
     4261 ttgtaacaaa tttatcaggg aactctttat tgtaattctt agcataaaaa acaggcttgc
     4321 taaaatgttt tctgtaagaa tctatttcta aagaaggtat gtttttattg gtagcttcaa
     4381 gctctttaat tatttcaatt aactgcatat attactcctg aaagtattat attgcaacag
     4441 catttgacat tctttgaaat tcatatagtg catagtatat ttgtatatat gattttgcgt
     4501 caacaaaata agataatttt cacaattatt ttatcatgaa agttgttttt ctgcaaaaaa
     4561 ttgtaaaata tgaaaatatg atggaagata ttgaaaactt tactttttga ttccacggtg
     4621 aataaaaaat gaagataaaa tcccttgttt caattgttca tcagatgaaa atataagttt
     4681 ttagagttgt ccataaatat aaaaaacgtc aaactataaa actcttgact ataatttagt
     4741 tattttttta gtgtaaataa aaggagttac gtgatgaaaa ctgctaacat tcctgaattg
     4801 attgaagaga tacgcaatgg aagaatgatt atccttgttg ataatgagga tagagaaaat
     4861 gaaggggatc tggttgttgc cgctcaatat tgcactccgg ataaaattaa ttttatggct
     4921 acgcatggca aaggcttgat atgtattgcc atgacacagg aacgtctgca agaattggga
     4981 ttgccactga tggttcccga aaattcagat aagttttgta cagcatttac cgtttcagtt
     5041 gatgccaaag aaggaacgtc aaccggtatt tctgcctatg atagggcaaa gacggtgcat
     5101 gcattgattg atccacgcac caaaccgcag gatttaaaca agccggggca tattttcccc
     5161 cttactgcac gaaagggcgg cgtgctggta cgagcagggc atactgaagg gtcttttgat
     5221 ttaacccgtt tggcaggttt atatcctgct gctgttatgt gcgagatatt aaatgatgat
     5281 gggacgatgg cccggcgccc cgatctggat gcatttgctg aaaagcacaa tttaaaaatt
     5341 ggaaccatcg aagatttaat aaaatttcgc cagcataaag aacgtcttgt acatagagtt
     5401 tcagaggcac acttaccaac ggagtatggt gactttaaaa ttattgccta tgaaacagag
     5461 gtggataatg ctacgcatgt tgcactggta aaaggagagg ttgatggcag ggaaaatgta
     5521 cttgtcaggg tacattcaga gtgtttaacc ggtgatgtct ttggttcgca caggtgtgat
     5581 tgcgggtcac agttgcatca agcgctcagg atgatagaaa aagaagggtt gggcacgctg
     5641 gtatatatgc gtcaggaagg ccgcgggata gggctcagta ataaattaaa ggcatatcat
     5701 cttcaggagc aggggtatga tacggttgag gcaaatataa agttaggatt tcctgctgat
     5761 cttcgtaatt atggtattgg ggcacagata cttgttgatc tgggactgtc aacaatacgt
     5821 ctgatgacaa acaatccaaa aaaggtgatt ggattggaag gatatgggct taccattgtg
     5881 gaacgtgtcc ccatcgaaat tccacctata aaagaaaact atcgctacct aaaaacaaaa
     5941 aaagataaat taggacatct aatattacaa gctcaagagg ataagaagtc ttgaaaaatg
     6001 gacaggcgcc cacaatagta gttgttgacg atgatgaggt aattcgctat agcctccaga
     6061 aaaagttgtc aagattaggt tacaacatta tttcattaag caaagcggaa gatgctcttt
     6121 tcctgttgaa aaatgatgaa cgggagatag accttataat aacggatata aaacttagaa
     6181 aaatggatgg tattgagtta ttgcgtcatg ttaatgttag cgaaaatcct gtacctacac
     6241 ttattataac agggcaggga aatattgaag atgcaattaa agcgttacga tatggtgcac
     6301 tggattttat cagaaaacca tttgacatga atgaggttgc ttcggttgtc aggaatattt
     6361 tgagaagaag acaacagcat agccttgaga atacattggg acagtatatt gttgatgatc
     6421 atagaacgct tgtcatccca atagatgaaa caattgctga tcttgttgcc tacgaattaa
     6481 caaaacactt atcgtatgca aagttttgta atcgttcaac ggctgaaaat atatcaatgg
     6541 cacttcttga agcgctttcc aacgcaatgt accatggcaa tttagagata ccctctgagg
     6601 tgcgtgagga gcacggggtg aaaggatttc atgatgaaat acaaaagcgc ttgcaggatt
     6661 ctcagtataa tcaacgaaag gttaccatac gttatagcta tacaggggaa tatgttgaat
     6721 atattataga ggatgagggc aaaggttttg attataaaaa tgttgcagat ccacgcgacc
     6781 ccgaaaactt tttgaaaaat agcgggagag gactttttat tattcaggtt catatggatg
     6841 aggttgaatg ggataatggt ggcagacgga tacgcatgcg aaaatataaa acactgtaat
     6901 gaggtgctaa catgtactat actataaaat gggatgatac agccgtttca atgcttgatc
     6961 aacgtatgtt accggtacag gaaatttacc ggcattacac caattatcat gatgtagctg
     7021 atgcaattcg ttctatggtt atacgtggag cgccagctat tggtattgca gcagctatgg
     7081 ggatagccct tgcagcggta aaaaacaagc tatcagttca tgatgaattt aaaactgaga
     7141 tggtaagggt atgtgatgag tttgcacaaa cccgccccac agcagtaaac cttttctggg
     7201 caataagccg tatgaaaaca attattgaaa aggcaactga tattcagcat tgcaaagaag
     7261 cccttgttga tgaggcaaag aagattcatc aggaagatat tgccataaac aaaacaattg
     7321 gtgaaaacgg acaaaagttt ctggatgatg gtatgacggt gatgacatac tgcaatgcgg
     7381 gtgcgctggc taccggaggt tatggtactg cgctgggtgt tatacgcagc gccatagagc
     7441 atgggaaaaa aataaaagtg tatgcctgtg aaacccgtcc ttacttacag ggtgcacgta
     7501 tcaccgcatg ggagctttat aaagatggca ttgaaacaac gcttatctgt gataatatgg
     7561 cagggtactt tatgaaaaaa ggagaaatta actgtgtgat agttggtgct gatagaattg
     7621 ctgcaaatgg cgatacggca aacaagatag gtacctacac aatgtccctg cttgcaaagg
     7681 cgcatgccat tccattttat gttgccgctc cgctatcaac cttagataca aataccatta
     7741 atggcgatag tattattata gaagaacgca gcattgatga ggtggcatat attcagggtg
     7801 tgcagattgc accggagggc atatttatta aaaatcccgc atttgatata acgccggctg
     7861 acaatataac agctattatt actgaacagg gaatagtaga gcatcctgct gcaactggca
     7921 tagccccgct ttttagtgtg cgatagttac tattaaatta aaagctgtat accactgcaa
     7981 taaaataaat ttttttagtt gcttttattc ataatgtgta ggagtgtaat ctatgttata
     8041 gcgaaaaaca gccatctggg tgaaatcgtt aaaaaagaga ttaaaactct tgaagcctta
     8101 aagcaattga acgggtagca gtttaaattt gtgggcaatg tcattcccgc gaaggcggga
     8161 atctaataag gcatgtcgag taaaaatgaa taaatattac atatatatga tcaccaatag
     8221 aaataataat gtccttttca ttggtgtaac aaatgacttg aaacgaaggg tttttgagca
     8281 cagggagaaa ctggtggaag gatttagctc aaaatataat tgcagcaaac ttgtgtggta
     8341 tgagcaaacg aataatattg gaagcgctat tctaagagaa aagcagatga aaaaatggaa
     8401 aagggaatat aacaataaca ttataaatga gatgaatccc gaatggaatg atttatatga
     8461 gactattcta tgagattcct gcattcgcag gaatgacaga aagtatgcag gaatgacaga
     8521 agtgtataaa tctattatgg gaggaattat gaggcaggct atcagtatta tacctgttat
     8581 acttagtatg gtgctgtatg gtacaatcgg tgttgcaagg gacattccct acacacttga
     8641 agatagagaa cggttgatac gggttgaaac aaaattagaa gaaggctaca aggctttgca
     8701 tagcaggatt gatgcggtgg ataaaaggat tgatgcggtg gataaacgca tagatgatgt
     8761 taaggggttg ctgtacgttt taatagcagc aatatttgca caaactgttg gagtagttgg
     8821 ctttgtagta tgggaccgac gaaccgcatt atcacctgct attaataaaa caaaagagat
     8881 tgaagaaaaa gaagataaga ttattactgc attgaaacaa tcggcaatga aggataagaa
     8941 tattgcagaa gctttgcgaa aggcagggat gctttaatag tccaaaatgt ccattatatg
     9001 taatagtaat atagtaatat tatctctttc ccatacagta gtgctgctat aaatcctaat
     9061 gtaagaaacg gtgcaaatgg tatttttgct ttcaatgtct t
//
LOCUS       DAOSOX010000090         8979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_7734_length_8979_cov_8.138615, whole genome shotgun sequence.
ACCESSION   DAOSOX010000090 DAOSOX010000000
VERSION     DAOSOX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7734_length_8979_cov_8.138615"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLX22_07565"
     CDS             complement(<1..623)
                     /locus_tag="PLX22_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19796.1"
                     /translation="MLKEYFASIQNTISGLQSSKDSKKASKKNQVREEFDGKKIMKLL
                     QDIVPNEFIVSDKKPVDASGYVPKTVDYIAYYKKWTIVEEMMNGHIPSELVYATFHIT
                     KHIDKQSLSEVLSNVTLTKKLNKYAELDQDIIIPAFVIAQSSDIDVPQLKNDVLDYYV
                     DYSVDSTFEFDIMMIIGKGIIIKDWREKRKFVALETEEDTLLWFFIL"
     gene            complement(830..2062)
                     /locus_tag="PLX22_07570"
     CDS             complement(830..2062)
                     /locus_tag="PLX22_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19797.1"
                     /translation="MKTKTLAGALLIVIGILLIIYYVLVIQPQSEAKKLLTEATLIYE
                     RNDKESINQAVTILTKIIARYPDTKTAIEAYYYIGQCYEKLNLNRLAYLKYVYLVKNN
                     RKNLSDEMYKNILARIAHINWLQNYSEESAHQLLTLLHASMDRNYRSKIYSELGHLYL
                     KNGEIQKAKRMFDIAINEDSSNEEAILGRARSLKHLGKDAEAYNHYDYFLRYYGAVSQ
                     YVNDVKNAYKVQAYKSGLNAFRSGHYNDAIGFFNRVLIHFGGDKLSENALYWIGESYF
                     AMRQFDRAIAYFNKALTNEFYHKDQDAQIKKGYAYFMQKKFDLAAREFQRYIREFPKG
                     RYITVAKEWKNMSTAELQNIIEAKKAKSEEYIEEESQQKPQPQKNNTNENDLGLEKDD
                     EVVGSVSQDFNDVNVAEF"
     gene            complement(2091..3890)
                     /locus_tag="PLX22_07575"
     CDS             complement(2091..3890)
                     /locus_tag="PLX22_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19798.1"
                     /translation="MMRNKLFILCFVTFILLSGGFIVYAQFFIDDNNTNIKTSPGLPD
                     YLARSIKAELIDGVEGAVRIVFSVDPNSKCDFLLGRTTRVPSTPEVALQATTVKVVLA
                     GAEPVAIDSNLPPGQYYYVVLARDKIVEKDIELYPDVNYTSAPIVIKKKIPELPAKRL
                     YDHVTLLYARVVNKTQVLLTWKGIQQPNIVYNVYRATEPLNSPGKIKNARLVGEIQSD
                     VESYIDRTIAQTGKYYYAVTVKDMEGNEDIQLIPDQSYTSAPVSVILRRDVFVTGIKA
                     TLDNDYLKVSWNKPDIDVKNFVIYRHTEPITDAQDLALATKVATVDAKRSHYIDTHPI
                     PGTYYFAVLAILEDSSIDTTLKPDMNYTTVPITVGHPISLKLLEAIEQNGLVKISWAY
                     TGSSGNRYFKLFRLRNAPFSVQDLNNAYLVDVINITNNEYIDSNIPAGSYYYALVPET
                     YDNELKIIKGVNCTAESVKVTKMAKAQLDLQDPEKEKTGKSELPESQKEEAKPYQLEK
                     QKLSATADINKIIAITFYKGKYETCIDTLSKYSEQSKNSIEIAKANLYIGRSYIELKQ
                     YKKALHYLMQKDVATYFPKDAKFWQDFAIARSQ"
     gene            4271..4861
                     /locus_tag="PLX22_07580"
     CDS             4271..4861
                     /locus_tag="PLX22_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT19799.1"
                     /translation="MDKQEKIHKKKIIILEALKRLLQNDVYSRITVQAVADEAGFSKG
                     GLLHYFPTKEEMYIEFMEYLFNEINNDHKNVLKGNLHSKAKASISAMYGVERFFLDPA
                     TAKILINLMLYAYEDEKIMVRLKEFIRRHLELYKTIINEAREDQPQRRKTDFDAEFHA
                     RIAQIIVMCAGLFESIDPLPIDNLNFMRYIISLFRG"
     gene            5240..6808
                     /locus_tag="PLX22_07585"
     CDS             5240..6808
                     /locus_tag="PLX22_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19800.1"
                     /translation="MMNIITHATNNGTNNKTREELFHEIIFNNHKRLFKAFTGITVLA
                     LLATLIIFLTGSGSHYLTLFDIIITGIIVGFILLSGFLLLTKYRNEWFSPYLAIFIVM
                     VTLFIFQYIIYGSRELFAAHYIILALSVFYFDTKVAGFAFVSVVLSQIVLFVCRPQLI
                     PEGPIGSIIGVRFLIYIWVGISAVFGTKATRALLDLALDKADEAQNKQERLVVVAQNV
                     EYSVKVLNEKSLEQTNVVNTINDLANKQAASLEEISASVEELTSNAENISGTARTLYQ
                     EMQIANESVNDVKKVFEEITKSGATVAHAIETITVLSSDAIQKMDLTLNQFHDLITFS
                     NTMSSFVEVINRIAEQVNLLSLNASIEAARAGEAGRGFAVVADEISKLADATAQNASQ
                     IEKIISQTQNLIGSSNNSITDTSQFLSKLNGEIRTITHEIAHTNTLIQDVNVSIKTIA
                     NLNNQIYNAIEKIETSTNEQRVANEEASKTIVYISDAAQNLTDIITTLSQISRSIEDI
                     AGSLNTLTQAMIQS"
     gene            complement(6813..7220)
                     /locus_tag="PLX22_07590"
     CDS             complement(6813..7220)
                     /locus_tag="PLX22_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOT19801.1"
                     /translation="MEFIEWTNALSVGVTTFDNEHKQLVVLINTLNQALTAGSAKKTM
                     EDILKSLVNYTTIHFKHEEDYMVLYDYPDYQKHKKEHDALTTQVIEFQERYRAGKTAF
                     SLELMYFLKDWLINHIMGTDKKYKDYFIAKGVQ"
     gene            complement(7230..7730)
                     /locus_tag="PLX22_07595"
     CDS             complement(7230..7730)
                     /locus_tag="PLX22_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOT19802.1"
                     /translation="MKKKYIIILLAIVIVNVFVFSIFSQVKWYDFNAGYSAIKNSKKP
                     AVIDFYADWCIWCKTMDKETFGNPMVTKKMSKDYIAIRVDVEKNETIVYNNNKFTAQE
                     FAAYCGVEGLPTLVFMDKEGQLVTRIPGYIKSDVFLSLLDYMKGECYKKKVSFSDYMK
                     DKGCNK"
     gene            8261..>8979
                     /locus_tag="PLX22_07600"
     CDS             8261..>8979
                     /locus_tag="PLX22_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT19803.1"
                     /translation="MFIKRILSACLIVSIFILFLTLTGCDNDIFKYSIHEIDSKYSGS
                     NFNAINAQLIESLYNNPFEDFTFAVVTDSHSDYDKLDSAVRLINANPSIKFVIHLGDF
                     TETGLLLQYEMTVHILNKLIVPFVVAIGNHDLLGNGFSIFKDIFGPTNFSFTCNDVFF
                     IVGNNNNWESSIGDILQQFNDLLVNNQGAQYRIIATHIPFIDDARYFQEFLDNYQYLC
                     EEYNVALSLHGHKHRHYYIPD"
BASE COUNT         2872 a         1592 c         1366 g         3149 t
ORIGIN      
        1 atcaggataa aaaaccacag caaggtatct tcctcggttt caagtgcaac aaattttcgt
       61 ttttcacgcc aatccttaat aattatacct ttcccaatga tcatcataat atcaaattca
      121 aacgtactat caacactata gtcaacataa taatcaagga catcattttt tagttgtggc
      181 acatctatat cgcttgattg agcaataaca aatgctggaa taataatatc ctgatcaagt
      241 tcagcatatt tattcaattt tttggtcaag gttacgttac ttaaaacctc tgataaactt
      301 tgtttatcaa tgtgttttgt tatatggaag gtagcataca ctaattctga tggaatatgc
      361 ccattcatca tttcttcaac gatagtccat tttttataat atgctatata atcaactgtt
      421 ttaggtacat atccgctagc atcaacaggt tttttatcag atacaataaa ctcattggga
      481 actatatcct gtaacagttt cataattttc ttaccatcaa attcttctct gacttgattt
      541 tttttggatg cctttttact atctttagaa gattgcaatc ctgatatggt attctgtata
      601 cttgcaaagt actcttttaa cattgaaatt ctccacctga aattaacttt ttcttaaaat
      661 aactgatact ggacattgta gcactgctca tatgcaacaa tcatatattc tactcaaatt
      721 aaatcatcat tgattaagta taatattgcc aatacataaa atcaatatta tttcacattg
      781 tttatagtaa ttgctattat tggctattgt tgaccggatt acaatttagt taaaattccg
      841 ccacattaac atcattaaaa tcctgtgaaa ccgaacccac cacttcatca tctttttcaa
      901 gacctaaatc attttcgttg gtgttattct tttgtggctg cggtttttgt tgcgattctt
      961 cttcaatgta ttcctcgctt ttagcttttt tagcttcaat gatattttgt aattcagcag
     1021 tactcatgtt tttccattct tttgcaacag tgatatacct gcctttgggg aattcgcgaa
     1081 tatatcgttg aaattcacgt gcagccagat caaatttttt ctgcataaaa taggcatacc
     1141 cttttttaat ctgggcatcc tgatctttat gataaaattc atttgttagt gctttattaa
     1201 aataagcaat ggctctgtca aactgtcgca ttgcaaaata tgattcgcct atccagtata
     1261 atgcattttc tgataattta tcgccaccaa aatgtatcaa taccctgtta aaaaatccta
     1321 ttgcatcatt gtaatgacca ctcctgaaag cattaagacc acttttatat gcctgtacct
     1381 tgtatgcatt ttttacatca ttgacatact ggcttaccgc accataatac cgtagaaagt
     1441 aatcatagtg attatatgct tcagcatcct tccctaaatg ctttaatgaa cgtgctcttc
     1501 ctaaaattgc ttcttcattg ctgctatcct catttatagc aatatcaaac atacgttttg
     1561 ctttttgtat ctcgccattt ttcagataca aatgccccaa ttctgaatat atcttacttc
     1621 tatagtttct gtccattgaa gcatggagca gggttaataa ttgatgagcg ctttcttcac
     1681 tataattctg caaccagttg atgtgagcta tacgtgcaag tatgttttta tacatctcgt
     1741 cagataaatt ctttctgtta tttttcacca gatatacata cttgagatag gcaagcctgt
     1801 taaggtttaa tttttcataa cattgcccta tatagtaata ggcttcaatt gctgttttag
     1861 tatcaggata ccgtgcaatg atctttgtca aaatggtaac agcctgatta atggattctt
     1921 tatcatttct ctcataaata agcgttgctt cggtaagtaa cttttttgcc tcgctctgag
     1981 gttgtattac caggacataa tatattatta acaatatccc aataacaatc aataatgccc
     2041 ctgctaatgt ctttgttttc atagttacct cgttactcca tatcataaat ttactgcgac
     2101 cgtgctattg caaaatcttg ccagaacttt gcatctttag gaaaatatgt tgctacatct
     2161 ttttgcatta aatagtgtaa cgctttttta tactgcttta attcaatgta acttctgcca
     2221 atatagagat tggcttttgc aatctcaata ctattcttgg attgttcact gtatttgctt
     2281 aacgtatcaa tacaggtttc atattttcct ttataaaagg ttatcgcaat tattttgtta
     2341 atatctgcgg tagctgataa tttttgcttt tccagttgat agggtttggc ttcttccttt
     2401 tgagattcag gcaattctga tttgccagtc ttttcttttt caggatcttg taaatccagt
     2461 tgtgcttttg ccatttttgt aacctttact gactctgcag tgcaattaac accctttata
     2521 attttcaatt cattatcata cgtttccgga accagagcat aataatatga accagcgggg
     2581 atgttgctat caatatattc attatttgta atattgataa catcaacaag atatgcattg
     2641 tttaaatcct gtactgagaa gggagcatta cgcaatctga aaagtttaaa atacctgttc
     2701 cctgatgaac ctgtataagc ccatgatatt tttaccaatc cattctgttc aatagcttct
     2761 aatagtttta ggctaatagg atgaccaacc gtaataggaa cagtagtata attcatatca
     2821 ggttttaagg tagtatcaat agaactatct tcaagtattg ctaatactgc aaaatagtaa
     2881 gtacccggaa ttggatgagt atctatatag tgggatcgtt ttgcatcaac agttgcaacc
     2941 tttgttgcta atgccaaatc ctgagcatca gtgataggct cagtatgcct atatataaca
     3001 aaatttttaa catcaatatc tggtttattc cagctcactt taagatagtc attatcaagt
     3061 gtagccttga tacccgtcac aaatacatca cgccgtaaaa taacagaaac aggtgcagaa
     3121 gtatagctct gatcaggtat tagttgaata tcttcattgc cttccatatc tttaactgta
     3181 actgcataat agtatttccc agtttgtgcg atagttctgt ctatatagct ttctacatca
     3241 gattgaatct cccctaccaa tcgtgcattt tttatttttc ccggactatt taaaggttca
     3301 gttgccctat aaacattata gacaatattt ggttgttgaa tacctttcca tgttaacaac
     3361 acctgagttt tattaacaac acgtgcatag agcaaggtaa catgatcata caaacgcttt
     3421 gctggtaatt cagggatctt ctttttaatt actattggcg cactggtgta atttacatca
     3481 gggtatagtt caatatcttt ttcaacaatc ttatcccttg ctaaaacaac ataataatat
     3541 tgacctggcg gtagatttga atcgatagca acgggttctg ctcctgctaa aactactttt
     3601 actgttgtag cctgtaatgc cacctctggt gtggaaggaa cccgggttgt ccttccaagt
     3661 aaaaagtcac attttgaatt gggatcaaca gaaaatacta ttcgtaccgc accttcaaca
     3721 ccatctatta attctgcttt aatactacgc gctagataat ctggtaatcc tggtgaggtt
     3781 tttatgttgg tattattatc atcaataaaa aattgtgcgt aaacaataaa acctccggat
     3841 agcagaataa atgtaacaaa acagagaata aatagcttat ttctcatcat gaaggcaatt
     3901 ttccttatct acttgctaaa ttaattttaa tttacagtaa ctatcgcaga gcaatagccc
     3961 tataaaaaac tctcttatta cactatttcg gaaatattta tacaacactt gatgtattat
     4021 taaaaaatta aaaatgctaa ataaaaaaat tagggctcct aagaaaaata cttgacctgt
     4081 taagctatat ctgttaacaa taatagccat ttgaaagtac tattggttat gcagtaatgg
     4141 caaaaatgtt ttatataact ggtgccattt tgtaacacga gaataaaatt aatcatgtat
     4201 gttttctgct atatttcact actgaaaata tttactattt ttttataaat tttattgtaa
     4261 aataatagct atggataaac aagaaaaaat acacaaaaag aaaattatta ttctggaagc
     4321 tcttaaacgt ttgttgcaaa atgatgttta ttcgcgtatc acggtgcagg ctgttgctga
     4381 tgaagcaggt ttttcaaagg ggggattgtt acattacttt cctactaaag aagaaatgta
     4441 tattgaattt atggaatacc tttttaatga aattaataac gaccataaga atgtgctgaa
     4501 aggtaatctg cattcaaaag ctaaagcaag tatttccgcc atgtatggtg tcgaacgttt
     4561 ttttcttgat ccggctactg caaagatatt aatcaacctg atgttatatg cttatgaaga
     4621 tgaaaaaata atggtacgtc ttaaagaatt tatacgccgt catctggaat tatataaaac
     4681 cattataaat gaagcacgtg aagatcagcc acaacgcaga aaaactgact ttgatgctga
     4741 atttcatgct cgtattgcac agatcatcgt tatgtgtgca gggctttttg aatctattga
     4801 cccgctcccc attgataact taaactttat gcgttacatt atctctcttt tcagaggata
     4861 gagcattgat acagcattat tgaacattta gatcgtcata aacacgggaa aatagtgcct
     4921 caatctctat gtcgatatct ccataaacga atacctgtat tgttatttac tcatgatgct
     4981 ttctttatgt aacgtaatag taccatggtt ctataccctt tcatatttta acggaaagcc
     5041 atggccatgc aacattaatt acactatgct aaactcctgg cacaatcaaa taaacctata
     5101 aacgtataaa ttgtatagta tatttttcat gacatataat gctgaaaagt catttattat
     5161 ttgacagttt tacttattat actataatat gactaaaata tgactaaggc attaactctt
     5221 tatgaacaaa gaggactata tgatgaacat aattactcat gctacaaata atggtacaaa
     5281 taataaaacc agagaagagc tatttcatga aataattttt aataatcata aacgcctttt
     5341 taaagctttt accggtatta ccgtattggc attattagcc acgttaatta tttttttaac
     5401 cggttcgggt tcacactatt taaccttgtt tgatattatt attaccggaa tcattgtagg
     5461 ttttatttta ctatcaggat ttttattact tacaaaatac cgtaatgaat ggttctctcc
     5521 atatcttgca atttttattg tgatggttac attatttatt tttcagtata ttatctatgg
     5581 ttcacgagag ctttttgcag ctcattacat catattagca ttgtcagtat tttactttga
     5641 tacaaaagta gctggttttg cttttgtatc ggtagtgtta tctcagattg tactttttgt
     5701 ttgccggcct caactaatac cggaaggacc tataggcagt attatcggtg tacggttttt
     5761 gatctacata tgggtgggta tatcggctgt atttggaaca aaagctacac gagctcttct
     5821 tgatttagcc cttgataaag ctgatgaagc tcaaaacaaa caagaaaggc ttgtagtcgt
     5881 tgcccaaaat gttgaatatt cggtaaaggt acttaatgaa aaatcccttg aacaaacaaa
     5941 tgttgttaat accatcaatg atttagccaa caaacaggct gcatcccttg aagagatatc
     6001 tgcgtcggtt gaggagctta ccagtaatgc agaaaatatc agtggtactg ctcgcacatt
     6061 atatcaggag atgcaaatcg caaatgaatc agtaaacgat gtaaaaaagg tatttgaaga
     6121 aataacgaaa agcggggcta cagtggctca tgctattgaa actattactg tattatcaag
     6181 cgatgcaatc cagaaaatgg atttaacact gaatcagttt catgatctca ttacgtttag
     6241 caataccatg tcatcatttg tagaagtaat caaccggata gcggagcagg ttaatctttt
     6301 gtcgctcaat gcatccattg aagctgcgcg tgcaggtgag gctgggaggg gatttgcagt
     6361 ggtagcagat gaaatatcca aattagctga tgcaacagca cagaatgctt cgcagataga
     6421 aaaaattatc tcacaaactc agaatttaat aggttcaagt aataacagta ttaccgatac
     6481 atcacaattt ttatcaaaac taaacggaga aatacgtact ataacacacg aaatagcaca
     6541 taccaatact cttattcagg atgtgaatgt ttcaataaaa actatcgcaa accttaataa
     6601 tcaaatatac aacgcaattg aaaaaattga aacatcaaca aatgaacaac gggttgccaa
     6661 cgaagaagca tcaaaaacaa ttgtgtatat ttctgacgca gcacaaaacc tcaccgatat
     6721 cattaccact ctatcacaga tatccagatc aatagaggat attgcaggaa gtcttaatac
     6781 attaacgcaa gcaatgatac aatcataagg ggctattgta caccctttgc aatgaaataa
     6841 tctttatact ttttgtcagt acccatgata tgattgataa gccagtcttt taaaaaatac
     6901 atcaactcta atgaaaatgc agttttgcca gctctatagc gttcctggaa ttctatgacc
     6961 tgcgtggtga gcgcatcatg ttctttttta tgtttctgat aatcgggata atcataaaga
     7021 accatataat cttcttcatg cttaaaatgt attgttgtat agttaaccag acttttaaga
     7081 atatcttcca ttgttttttt tgcgcttcct gctgttaaag cttgatttaa tgtattgatt
     7141 aatactacca gttgtttatg ctcattatca aatgttgtta cgccaacact cagcgcatta
     7201 gtccactcaa taaattccat aaccacccct tatttattac aacctttatc tttcatataa
     7261 tcactgaacg ataccttttt tttatagcat tcacctttca tataatcaag taacgataaa
     7321 aaaacatctg atttaatata tcccggaata cgggtaacaa gctgcccttc tttatccatg
     7381 aacacaagcg taggcaatcc ctcaacaccg caatatgcag caaattcctg tgctgtaaat
     7441 ttattattat tatatactat tgtttcattt ttctctacat ctacccgtat ggcaatatag
     7501 tccttactca ttttttttgt aaccattgga ttcccaaagg tttctttgtc catcgtttta
     7561 caccatatac accaatctgc ataaaaatca ataacggcag gctttttgct attttttatt
     7621 gcagagtacc ctgcgttaaa atcataccac ttaacctggc taaaaatact aaaaacaaac
     7681 acatttacaa taactatcgc caaaagaatt attatatatt tctttttcat attgtatatt
     7741 actccttcct attcttaatt ttgagaaacg taaatcataa attgctttat aaaagtttta
     7801 acgatagttt gttgttgcat ttcccctata ctattaaaac ttctattgtt caaagttgat
     7861 aatgtacaat agtttatgat aagctataat ctttccctta cattagtaaa aatactacat
     7921 agcaaaaagt aatctaaaaa aacattatgt tgcatccatg gcggatagta gagtacttat
     7981 ggcactgctt taatagttat aatagttttt ttgataaaaa agttatcgat tgtacaccca
     8041 tcattatcaa caaggcgtag caataccaaa aacctgcaat ataagcaatt ctttagtgcg
     8101 gtatgaaaag tgaagagttt ttgcggtttc atgttactgt taataaaagc atgatgtttt
     8161 atttggatat ttttacagta agtagtatga tttaattaag tagttaatta atacagcgac
     8221 agattgagca cagtatataa attaaacaaa aggggttggg gtgtttataa aacgtatact
     8281 atcggcatgc ctcattgtat caatttttat tttatttcta acacttactg gttgtgacaa
     8341 tgatattttt aaatatagca tccatgaaat tgatagtaaa tatagtggaa gtaactttaa
     8401 tgctataaat gctcaattaa ttgagtcgct atacaacaat ccctttgaag attttacctt
     8461 tgctgttgtt accgactctc atagcgacta tgataagctt gatagtgcag taaggcttat
     8521 caatgcaaat cctagcataa aatttgtaat tcatcttggt gattttaccg aaacgggatt
     8581 gctattgcaa tatgagatga ctgttcatat tttaaacaag ctgattgtac catttgtagt
     8641 agctataggc aatcatgatc ttcttggcaa tggattcagt atttttaagg atatttttgg
     8701 tcctacaaat ttttcattta cctgcaatga tgtttttttt atcgttggta ataataataa
     8761 ctgggaatca agcataggcg acatattaca acaatttaac gatctccttg ttaataacca
     8821 gggtgcacaa tatcgaatta tcgctactca tattcctttt attgatgatg cacgttattt
     8881 tcaggaattt ttagataact atcaatatct ttgtgaagaa tataatgttg cgctttccct
     8941 ccatggacac aagcatagac actactatat accggattt
//
LOCUS       DAOSOX010000091         8871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_7852_length_8871_cov_8.014065, whole genome shotgun sequence.
ACCESSION   DAOSOX010000091 DAOSOX010000000
VERSION     DAOSOX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_8871_cov_8.014065"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /gene="pheT"
                     /locus_tag="PLX22_07605"
     CDS             <1..1719
                     /gene="pheT"
                     /locus_tag="PLX22_07605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT19804.1"
                     /translation="DNITIKESPDWLKARVEAIGMRPINNIVDVTNYVMAELGEPMHA
                     FDRKKLRGNEIIVRFASEGEHLMTLDGQEHILTSQDVVIADSGGPIALAGVMGGGNSE
                     IEDNTTSIVLEAANFNPVYIRRTANRHNLRTEAAIRFEKSLDPEVCPDAILRCYELIK
                     MLLPEAEATTHIVDAYPVKAKPISITTTTDFIRRKLGHAIDDDTIVSILTSLDFKVNK
                     QDNNLTIDVPSYRATKDVSIPDDIVEEIGRIYGYDNIPQQAPLVPCAPPQQNIKRTFE
                     RTVKSILVNDYHMTEVSNYSFVGEALLNRLQMNEDTGLRLKNPLSKEQDRLRESLIPN
                     IILNITTNQRFFDDFRIFELGRVYHKQDRTSPDLAMENTRVAGIVYAKKPLYPLFYDA
                     KECCMGLFHKTKRTNYSINPACNQLPPYAHPARSLEIAIDNSPIALICELHPKIINEF
                     EIKGSVALFDVDIDLLLNSPVQETIFKELPRFPDVPFDISILADEKEYAATLVTMLYK
                     ADTTHIKNVEVISVYNQPPIPQGKKSVSFRIIFRADDHTLTPEEADSLQNKVIRFVEK
                     NGYKLR"
     gene            complement(1716..2417)
                     /locus_tag="PLX22_07610"
     CDS             complement(1716..2417)
                     /locus_tag="PLX22_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19805.1"
                     /translation="MKKIVGGVIAILLILFGFMMYISHKVIRINDMDIPDFQPLKDNT
                     LAEKYCPYIIANHEYEYPYAVYYRASIDNNGNTHIAYHFFWEREVNKTRGFVPWLTRN
                     LYTGGIKLQEIMFGKHDIEVIGVVLDKNHNIKKVIYESPEHYNPYDFIVKHKTNEIAD
                     TVHTPLRFKVVSWNHLFAFVDNNYNLKKGEVELYRKPQYFTKKLWDEFTMCKKEETAV
                     KRNRAHYAWEREYID"
     gene            complement(2498..5203)
                     /locus_tag="PLX22_07615"
     CDS             complement(2498..5203)
                     /locus_tag="PLX22_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT19806.1"
                     /translation="MMNRKLLIALTIFITGYILVYSIGKFYIDYRWFSIYNHLDIFWI
                     LFFSKFNVQTIFWGLFIALFILNFILIRILGGKGRIFTKNILDKIRIPGFGTTRNLLF
                     IILIIGVVVLGFFMGLWASTYWKEYLMFKNEVAFTDFPKEPLFNMDIGFFVFSLPFYK
                     FIFRWVLVSLSFITIFSFFFHILNNGIFSQNGKLEFSRFARAHLSILFGLIVLIIASG
                     YRLLAYDLLFNNRAKFFGAGYADVHAKLLAYDICMVISIIAAILLFANMVIRSFKMPI
                     ILLITLLPVYFVFGTIFPALQQRFIVDPNELEKEKPYIQYNINYTRIAFGLNGVKELP
                     FENAQTLTFRDLEKNRDVVNNIRLWDWRPLKETYKQLQGLKPYYDFIDVDIVRYTINN
                     QKVALNISARELDNTKLTVSKNSWLNNHLIFTHGYGVIASRVDKITPEGLPELVLYDI
                     PTKSTIPIPVTVPQIYYGEHQNSYIITNTKITPGEFDYPYGDENKYTIYNGTGGDVLN
                     SLVKRAMYAIAFGDFNILISNVITNQSRIHFRRNIVDMVTAITPFIYYDSDPYVVVVD
                     GKIYWIIDGYTYSSYFPYSTPIQLTNGVSINYIRNPIKIIIDAYNGTIRYYIADNNDP
                     VIKAYAKIFKNLFIPMEQMPDAFKDHLRYPEALFNIQAQQLLRYHMTNINVFYNNEDL
                     WDIPQQIYESAREKMHSYYLVTSLPNEPATEFLLILPFTPAKKDNMIAFMVARCDIPN
                     YGELRLYTLPKDKLNFGPMQIEARINQDAEISKQLSLWNQRGSRVIRGNMLVLPIRDS
                     ILFVEPLYLKAETSEMPELKRVIVSYKDKIIMEENLSLALSKLFTGGSFGERYYGGYA
                     IKDLVNKAYMHFIQAEEYQRQGNWAKYGEELKNLKETLTALRNAR"
     gene            complement(5220..6551)
                     /locus_tag="PLX22_07620"
     CDS             complement(5220..6551)
                     /locus_tag="PLX22_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19807.1"
                     /translation="MKQKNGFAKKELFAVILIALMNLFLFADQNLMAPNLTQIAHDFG
                     FNDVQRDVMLGGHISFVFWILGGLVTLAIGYLTDKISRRNLFLFVIIVGELPCLLTGF
                     AQNYDQLFWLRALTGIGIGGALPLTFSIIGDYFSHENRAAASAWIGLAQGLGIAVGQL
                     MAGFIGSAYGWRLPFIIVAIPNFILAFIFLLFVKEPQRGKTEESLKELIEEGKVYTGK
                     INWREYKSLFKIKTNILVFLQGIPGTVPWGVFFIFLNDFYSQDKGYSIEVATLIVLTV
                     GAAAILGAFIGGFIGNWLYNIKPKYLPMLCGTSTLLGIIPMALLLNYTPQVIIPEPDP
                     TLPLIYGFITGFTVTITGPNVRAILLNVNTPETRGSIFSLFNLTDDLGKGFGPVIISA
                     LIVLFGRLMAFNIANLFWLLCGLLLLVMIKTFPEDEARLNAILKEKAEKMK"
     gene            complement(6779..6952)
                     /locus_tag="PLX22_07625"
     CDS             complement(6779..6952)
                     /locus_tag="PLX22_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19808.1"
                     /translation="MYYSGIDPSWSGKYPTVVAVSNNSLKVVQYIYTKRVNDIICAIA
                     PYNNSMIEIDALV"
     gene            complement(7045..8715)
                     /locus_tag="PLX22_07630"
     CDS             complement(7045..8715)
                     /locus_tag="PLX22_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOT19809.1"
                     /translation="MEKIWLKSYAPGVPHTLKYDEITVVHGFERSAKDFPNKNAMIFL
                     DAKISFKKMQSMVNKIANALIDLGVKPGDKVAMLMPNMPQIVIGSYAAWKIGAVVVMN
                     NPLYTDHELEHQLNNSESTVLITLDLLGPRMIALRPKTQVKKIIVAHIRDHLGFPKKQ
                     LLPIVAKANHRNIPPTPEVYEWTDLLKKYPDKDPGTMAKFNDLATLQYTGGTTAVSKG
                     AMLSHKNLSSNVQQIKAWFPEFKRGELTSIGVLPYFHSFGMTTVMNISAFYGWTQVLI
                     PRPEAGAVLEALIKHKVDFMPAVPTFYVGLLNHPKIKEADISTIKGCFSGAAPLPVDV
                     IREFEAKTGAPICEGYGLSETTPVVTINPYGGKTIPGSIGLPIPDTECKIVDLDEGTK
                     EMEIGQEGELCIRGPQVMSGYYKMPDETAKTLRDGWLYTGDIAKVDENGYFYIVDRKK
                     DMIIAGGYNIYPRDIDEVLFEHPKILEACAIGVPDKYRGETVKAFVVLKPGETMTEQE
                     VIDYCKTKLAAYKVPKMVEFINELPKSNVGKILRKELRKMELEKMEKK"
BASE COUNT         2937 a         1699 c         1389 g         2846 t
ORIGIN      
        1 gataatatta ctataaaaga atcacctgac tggttaaaag cacgtgttga agcaattggt
       61 atgcgtccca tcaacaacat cgttgatgtt accaattatg tcatggcaga attaggcgaa
      121 ccaatgcatg catttgatcg taaaaaactg agaggtaatg aaattatagt taggtttgcc
      181 tctgaaggtg aacatcttat gacattagat ggacaggaac atatcttaac atcacaggat
      241 gttgttatcg ccgatagcgg tggtcctatt gccctggctg gtgttatggg tggtggaaac
      301 agcgaaattg aagacaatac tacatcgata gttctggaag cggcaaattt taatcccgta
      361 tacattcgaa gaacagcaaa ccgccacaac cttagaacgg aagctgcaat acgttttgaa
      421 aaatctcttg atcctgaagt atgccctgat gccatcctcc gttgttatga acttatcaaa
      481 atgcttttac ctgaagctga agcaacaaca cacattgttg atgcttatcc ggttaaggca
      541 aaacccataa gcataacaac aactactgat tttatacgcc gaaaacttgg tcatgctatt
      601 gatgatgaca ctatagtttc tattctaaca tcgcttgatt tcaaagttaa taaacaggat
      661 aataatctta ccattgatgt ccccagctac cgggcaacaa aagatgtttc aatccctgat
      721 gatattgttg aagagatagg caggatatac ggttatgata acatacctca acaggcacca
      781 ttagttccct gtgctccacc tcaacaaaat attaaacgga cttttgagcg aactgttaaa
      841 tcaattcttg tcaatgatta ccacatgacc gaggtaagca attattcttt tgttggtgaa
      901 gcactgttaa atagattaca gatgaatgaa gatacaggat tgcgattaaa aaatccatta
      961 tcaaaagagc aggacaggct cagagaaagc cttataccta atattatcct taacattact
     1021 accaatcagc gattttttga tgatttcaga atttttgaat tgggcagagt atatcataag
     1081 caggacagga cttcacctga tttagccatg gaaaatacgc gagttgccgg tatagtgtat
     1141 gcaaaaaaac cattgtatcc tttattctat gatgctaaag aatgttgtat gggattattt
     1201 cataaaacaa agagaacaaa ttatagcatc aatcctgctt gtaatcagct accgccatac
     1261 gctcatccag ctcgttcact ggagatagca attgataaca gtccaatagc attgatatgt
     1321 gaactgcatc ctaagatcat taatgaattt gaaatcaagg ggagcgtggc attatttgat
     1381 gttgatattg atttattatt gaactcaccg gttcaggaaa ctatatttaa agaattaccg
     1441 cgattcccgg atgtgccatt tgatatttca attctggcag atgaaaaaga atatgctgca
     1501 acattggtaa caatgttata caaggctgat acaactcata ttaaaaatgt tgaggtaatt
     1561 tcagtatata atcaaccgcc tataccgcag ggtaaaaaat cagtttcttt caggataatt
     1621 tttagggctg acgatcatac cttaacaccg gaagaagcag attccttgca aaacaaggtt
     1681 atacgttttg tggagaaaaa tggatataag ctccgttaat ctatatattc tcgttcccat
     1741 gcatagtgag ccctgtttcg tttcactgct gtttcttctt ttttacacat ggtaaactca
     1801 tcccacagct ttttagtaaa atactgcggc ttacgatata attcaacttc acctttcttc
     1861 aaattataat tattatctac aaatgcaaag agatgattcc atgaaacaac tttgaatcgt
     1921 aatggtgtat gtacagtatc tgctatttca tttgttttat gtttaacaat aaaatcataa
     1981 ggattataat gttcagggct ttcgtatata acttttttga tgttatgatt tttatccaaa
     2041 actacaccta tcacctcaat gtcatgtttg ccaaacatta tttcctgtaa ttttattcct
     2101 ccagtataaa gatttctggt aagccatgga acaaaacctc ttgttttatt aacctccctt
     2161 tcccagaaaa aatgatatgc gatatgtgta ttgccattgt tatcaatact ggcccggtaa
     2221 taaacagcat aaggatattc atattcatgg tttgctataa tataagggca atatttttca
     2281 gctaatgtat tgtcttttaa tggctggaaa tcagggatat ccatatcatt tatacgaatt
     2341 actttatgag aaatatacat cataaatcca aataatatta gcagtattgc tataaccccg
     2401 cctactattt tcttcatacc taatcccctg aaataaaatt attctccacc gtcattccgg
     2461 gcttgacccg gaatcaaaat atgtaatgac aaaagtatta tctggcattc cttaacgctg
     2521 ttaatgtttc ttttaaattc tttaattctt caccatactt agcccaattt ccctggcgtt
     2581 ggtactcctc agcctgtata aaatgcatat acgctttatt aactaaatct ttgattgcat
     2641 acccaccata atacctttcg ccaaagctac caccagtaaa tagctttgat aaagccaatg
     2701 ataaattctc ttccataatt attttatctt tgtaagatac tatcacacgc tttaattcgg
     2761 gcatttcact tgtttctgcc ttaagataca gtggttcaac aaaaagtatt gaatccctga
     2821 taggcaatac aagcatgttg ccacgaatta ctcgcgaacc acgttgattc cacagtgaca
     2881 gttgttttga tatttcagca tcctgattaa tacgggcttc tatctgcatt ggaccaaaat
     2941 tcagtttatc tttagggagg gtatataaac gcaactcacc ataattgggt atatcacatc
     3001 gagctaccat aaaagcaatc atattgtctt tctttgcagg tgtaaaaggt aaaattaata
     3061 aaaactctgt tgcaggttcg tttggtagcg atgttaccag atagtaacta tgcatctttt
     3121 ctcttgcact ttcataaatt tgctgtggta tgtcccataa atcttcattg ttataaaaaa
     3181 cattaatatt ggtcatatga tatcgcagta attgttgtgc ctgtatatta aatagtgctt
     3241 caggataccg aagatgatct ttaaaagcat caggcatctg ctccatggga atgaatagat
     3301 ttttaaatat ttttgcatat gcttttatta ccggatcatt attatcagct atataatacc
     3361 gtatagtccc attatatgca tcaataatta tctttatggg atttcttata taattaattg
     3421 atacaccatt tgttaattgt attggagttg aatatggaaa ataacttgaa taagtatacc
     3481 catctattat ccagtaaatc ttcccatcaa ctacaacaac atatggatca gaatcgtaat
     3541 aaataaacgg tgttatagcc gttaccatat ctacaatatt tcttctgaaa tgaatcctgc
     3601 tctggttggt gattacattt gaaataagta tattgaaatc accaaaagct atcgcataca
     3661 ttgctctttt cactagcgaa ttaagaacat ctccacccgt tccattgtag atggtatatt
     3721 tattttcatc accatatgga tagtcaaatt caccaggtgt tatctttgtg ttagtaataa
     3781 tatatgaatt ttgatgctca ccataatata tctgtggtac tgttaccggt attgggattg
     3841 tactttttgt tggaatatca taaagaacta attcaggtaa tccttcaggg gttattttat
     3901 caactctgct ggcaattaca ccataaccat gggtaaatat aagatgatta ttgagccatg
     3961 aattttttga aactgttagc tttgtattat caagttcacg ggcggaaata tttaacgcta
     4021 ccttttgatt attgatagta tagcgtacaa tatctacatc tatgaaatca taatacggct
     4081 ttaatccctg cagttgctta tatgtttcct ttaatggacg ccaatcccat aaacgtatat
     4141 tgttaactac atccctgttt ttttctaaat ctctgaaggt taatgtttgt gcattttcaa
     4201 acggcaactc tttaacacca ttcaatccaa atgctattct tgtataatta atattatatt
     4261 gtatatatgg tttttctttt tctaattcgt tgggatcaac aataaatcgt tgctgcaaag
     4321 ctggaaatat tgttccaaat acaaaatata cgggtaatag tgttattaac aatataatgg
     4381 gcattttgaa acttctaatg accatattag caaagagaag tatagctgca ataatagata
     4441 taaccatgca tatgtcatac gccaatagtt tagcatgaac atcggcgtat cctgccccaa
     4501 aaaattttgc acgattgtta aataaaagat cataagccag taatcggtag ccagatgcaa
     4561 tgattaaaac tatcaaacca aataaaattg acagatgagc ccgtgcaaac cttgaaaatt
     4621 ccagcttacc attttgagaa aaaatgccat tattgagtat atggaaaaaa aatgaaaaaa
     4681 tagtaatgaa tgacaatgaa accagcaccc agcgaaatat aaatttataa aaaggaaggg
     4741 aaaatacaaa aaaaccaata tccatgttaa ataagggctc ttttggaaaa tcggtgaaag
     4801 caacctcatt tttaaacatt aaatattctt tccagtaagt tgaagcccac aatcccataa
     4861 aaaaacccag aacaacaacc ccaataataa gtataataaa aagtaaatta cgtgttgttc
     4921 caaaacccgg tatcctgatt ttatcaagga tgttttttgt aaagatcctc cctttaccgc
     4981 ccagtattcg tatcaaaata aaattaagaa taaacagggc aataaataaa ccccagaaaa
     5041 ttgtttgcac attgaatttt gagaaaaata aaatccagaa tatatctaaa tgattataaa
     5101 ttgaaaacca tcggtaatct atataaaatt taccaattga ataaactaga atatatcctg
     5161 tgataaaaat ggttaatgca atcaaaagtt tacgattcat catatcacct tgctaatttt
     5221 tacttcatct tttcagcttt ttctttcaaa attgcattga ggcgcgcttc atcttcagga
     5281 aatgttttaa tcataaccaa aagtaataat ccacacaaaa gccagaaaag atttgctata
     5341 ttaaatgcca ttaaacggcc aaacagtaca atgagtgcac ttataataac aggtccaaaa
     5401 cccttaccca gatcatccgt gaggttaaat aatgaaaaga tagaacctcg tgtttcagga
     5461 gtatttacat tcagcaatat tgcgcgtaca ttaggcccgg tgatagttac cgtaaatcca
     5521 gttataaagc cataaatcaa tggtaacgtg ggatctggtt caggtatgat aacctgaggc
     5581 gtgtaattta gtaacaatgc cattggaata ataccaagaa gcgttgaggt gccgcacagc
     5641 attggaagat atttgggttt gatattatac aaccagttac caataaaacc tccaataaat
     5701 gcccccaata ttgccgcagc acctactgtc aatacaataa gtgttgctac ttctattgag
     5761 taacccttgt cctgtgaata aaaatcattc agaaaaataa agaaaacacc ccatggtacg
     5821 gttccaggta tcccctgtag aaataccaga atatttgttt ttattttaaa gagcgattta
     5881 tattcacgcc agttgatttt cccagtgtaa acctttcctt cttcaataag ttcttttaaa
     5941 ctttcttcgg tttttccacg ttgaggttct ttaacaaaca acaaaaatat gaaagccaat
     6001 ataaaattag gaatggcaac aatgataaaa ggtaagcgcc agccatatgc tgaaccaata
     6061 aaaccagcca taagttgccc tacagcaata ccaagcccct gagccaatcc aatccatgca
     6121 gaagcagcag ccctgttttc atgtgaaaaa taatcaccaa taattgaaaa tgttaaggga
     6181 agtgcaccac caatgccaat accggtcaat gcccttaacc agaacaactg atcatagttt
     6241 tgagcaaaac cggttaataa gcatggtaat tcaccaacaa tgataacaaa taaaaataaa
     6301 tttcgccgtg atatcttatc tgtcaaatag cctattgcta atgttaccaa tcctcccaga
     6361 atccagaata caaatgagat gtgcccaccc agcatgacat cgcgttgtac atcattaaag
     6421 ccaaaatcat gcgcaatctg cgttaaattg ggagccatta aattttgatc ggcaaacaaa
     6481 aaaagattca tcaatgcaat taagataaca gcaaacaact cttttttagc aaacccgttt
     6541 ttttgtttca tatatcaaac atcctccaaa aatataaact taacaccaat gtctttgtgg
     6601 gcaattaaca aaatgcattg taactatcat attataataa cgaaatcaag aattttttca
     6661 aatttattca acattctttt tacatggtct gttgtggttt tggcactatt actattcctt
     6721 cttctctact gccaaaataa ttgcaagcat tgtgcaaaca ataataaaca gtatatactc
     6781 aaacaagcgc atctatttca atcattgaat tgttataggg agctatcgca caaatgatgt
     6841 cattaactct tttagtatag atatattgaa ctacctttaa actattatta ctaaccgcta
     6901 caacggttgg atactttcca ctccatgatg gatcaatgcc actataatac attgttgtat
     6961 atcactattt aaaaaaataa aggggcattg taagtttatg cccctttttg attaaacaaa
     7021 ttgtatcaaa taaatcaaac taacttattt tttctccatt ttttctaatt ccatcttacg
     7081 aagttcttta cgcaatatct tcccaacatt gctttttggc agttcattga taaattcaac
     7141 catctttggt actttataag ctgccaattt tgttttgcaa taatctatga cctcttgttc
     7201 ggtcattgtt tcaccaggtt tcagtacaac aaatgcctta actgtttcac cacgatattt
     7261 gtccggcact ccaattgcac acgcttccag aatctttgga tgttcaaaaa gaacttcatc
     7321 aatgtcacgt ggataaatat tatatccacc tgctatgatc atatccttct ttctgtcaac
     7381 aatatagaaa tatccatttt catcaacttt agcaatatca ccggtataga gccatccatc
     7441 ccgtaatgtc tttgctgttt catcaggcat cttgtaatat ccgctcatta cctgtggtcc
     7501 tctaatgcat aattctcctt cctgtccaat ttccatctcc tttgtcccct catccagatc
     7561 aactatctta cattcagtgt cagggattgg caaaccaatg gaacctggaa ttgtcttccc
     7621 accataagga ttgatagtta caacaggagt tgtttcagat aagccgtacc cttcgcatat
     7681 aggtgcacct gtttttgctt caaactccct aataacatca accggtaacg gagcggcacc
     7741 tgaaaaacaa cctttgatag ttgaaatatc ggcttcttta atctttggat ggtttaaaag
     7801 tccaacatag aacgttggaa cagcaggcat aaaatcaact ttatgtttta ttaatgcttc
     7861 taatactgct cctgcctcag gacgtggtat tagaacctga gtccatccat aaaatgcact
     7921 aatattcata accgttgtca tgccaaatga gtggaaatat ggcaacaccc caatactggt
     7981 caattcacca cgtttaaatt caggaaacca tgcctttatc tgctgtacat tagacgaaag
     8041 gtttttatgt gaaagcattg cacccttact aacagcagtt gtaccacccg tatactgtag
     8101 ggttgccaga tcattaaatt tagccatagt tcccggatct ttatcaggat acttttttaa
     8161 aagatctgtc cattcatata cttctggcgt tggaggaata tttcgatgat tggctttagc
     8221 aacaattggt aaaagctgct ttttaggaaa tcctaaatga tcacggatat gagctacaat
     8281 aatctttttc acctgtgttt taggtcgcaa tgcaatcata cggggtccta atagatcaag
     8341 ggtaatgagc actgttgatt ctgagttatt caactggtgt tccaattcat gatccgtata
     8401 gagcggatta ttcataacca ctactgctcc tatcttccat gctgcatatg aaccaatgac
     8461 tatctgtggc atgtttggca tgagcattgc cactttatca cccggtttta cacctagatc
     8521 aatcaatgca ttggcaattt tattaaccat ggactgcatc tttttaaaac taattttcgc
     8581 atccagaaag atcattgcat ttttattggg aaaatccttt gcagatcttt caaaaccatg
     8641 cacaacggtt atctcatcat atttgagggt atgcgggaca cccggtgcat aagattttaa
     8701 ccaaatcttt tccattgcac aaacctcctg tgtcattttt gtaaattata attattaaga
     8761 atacatttac tggtaaaaat gatgaataat taaatacgac atatatagta ccatattaaa
     8821 aattattacc atacattgtc aaccaattat ttatatataa catttttatt c
//
LOCUS       DAOSOX010000092         8732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8026_length_8732_cov_12.021666, whole genome shotgun sequence.
ACCESSION   DAOSOX010000092 DAOSOX010000000
VERSION     DAOSOX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_8732_cov_12.021666"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..593
                     /locus_tag="PLX22_07635"
     CDS             174..593
                     /locus_tag="PLX22_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT19810.1"
                     /translation="MKSMTEYLTFNTRTKRDYINITTMVEDVVTRSGIKEGLCLVNAM
                     HITASVHINDDEKGLIHDIDDFLETLAPHEPISKYRHNRTGEDNGDAHIKRSIMGREV
                     VVAVTNGKLDFGPWEQIFYAEFDGQRPKRVLIKVIGE"
     gene            595..1362
                     /locus_tag="PLX22_07640"
     CDS             595..1362
                     /locus_tag="PLX22_07640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT19811.1"
                     /translation="MRLVDVHCHLESQHFSGRLDAIIDAARKVGVIAFITCAIVPDEW
                     ELSQTISHSFHEVYFAAGVHPWYIKNQYFEELQKLYAITDSKYIAIGEIGLDAKTDMD
                     LAVQKKFFEEQLAIANDLNKPVIVHCRGAFNELIASLKRIGKINAGGVIHSYSGNADI
                     ARQLIRYGFYFSFGGALTYRNSKKKLQVLHHIYPDHVLLETDSPDIPPVEKSGQINLP
                     ENIIYVVKAMSELIGNNEEEIAEATTQNAKKLLNLNI"
     gene            1423..1929
                     /locus_tag="PLX22_07645"
     CDS             1423..1929
                     /locus_tag="PLX22_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19812.1"
                     /translation="MDFETTTCISCKHLEILDSYCKKLDVSLRTCIVYMILYAAKKEK
                     KKAIAFKRICYRKRDKDNPWKRVHLVLYQSEYEFCLDVKKLWKMSLANIIAFCVENVL
                     VEFFEYFSRRLKEINSDNYPYNLTSYYENRSYTFDFHKEKGIHCLKFYWGPPPEVIEF
                     NKKQKKLL"
     gene            2100..2819
                     /locus_tag="PLX22_07650"
     CDS             2100..2819
                     /locus_tag="PLX22_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT19813.1"
                     /translation="MNINQNYYERFSRTSLLIGKENLEKLFTARVFIAGLGGVGSYAF
                     EALVRAGVGTVHIIDFDTVEISNCNRQLLALGSTIGIPKVDVAEKRAKDINPDGMINK
                     NKIRITPDNVHGLIPHDIDFAVDAIDDVDAKTSLITTLLGRHIPFISCMGAANACNPE
                     SLRVSDISKTQYCPLARTMRKRLKNIGIAGGFMCVYFHEERTGSTTNVSTNNTRGSLS
                     YMPGIMGLAAAGYAINFLIGR"
     gene            complement(2849..3646)
                     /locus_tag="PLX22_07655"
     CDS             complement(2849..3646)
                     /locus_tag="PLX22_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19814.1"
                     /translation="MQCANHIFSYTLKGGDFDNAGRISTSLKRELRTREYPSHMIQRV
                     AIVLYEAEINVVSYTTVGYFFAYCRPNSVHLVVKDKGKGIENIRLAVQEGFSTATDEI
                     RRLGFGAGMGLSNIKRNANVMRICSRPGEGTKICIDITSSNHYGDLIMDITVNEIQQK
                     TNFELLTTKADCTKGITGGYVGDMLSDVNANGKKGNIWITILTHPNAVAVASLKDFSA
                     IVVAGGIKPRDEFVKRADEEDIPVMYTDLSAYEVVVLLSSLGITANH"
     gene            complement(3659..4018)
                     /locus_tag="PLX22_07660"
     CDS             complement(3659..4018)
                     /locus_tag="PLX22_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT19815.1"
                     /translation="MKLSEIKKILDADVIVGEEMLNTVDVETAFASDLLSDVLAYAKE
                     RALFITGLTNPQVVRTVEVLDLIGVVFVRGKVPQMDTVELAKRKNIPLLRTKCIMFET
                     CGRLYGAGMKASIEKVD"
     gene            complement(4121..4732)
                     /locus_tag="PLX22_07665"
     CDS             complement(4121..4732)
                     /locus_tag="PLX22_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19816.1"
                     /translation="MKITKKRSDGLKRQSEIMMIALELFSKKGYHNTKLEDILKKADI
                     AKGTFYLHFNGKDDLLHMIVDSQLEEMYRILSVLDISMDKPLTEVINIYRTIAQYLIR
                     DPKIKTFARIMLKEAMGLDKTLLQKLNDFYNKIIAMSADYIAKAQGKGRVFPMLDPYF
                     TSMCIVGSIKELVFQWSVNDESLDLEKAIMTAADIYFRGMVKG"
     gene            5161..6495
                     /locus_tag="PLX22_07670"
     CDS             5161..6495
                     /locus_tag="PLX22_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19817.1"
                     /translation="MNLLSLFAPPPAKPQLPEEKVKKLYPRLRWHILQATFIGYATFY
                     IVRNNLSTVARDMQTAFGYDHSQIGSILAISALLYGIGKFLLGSVSDRSNVRVFMPVG
                     LILTALCNFAFGATQSYAIHFWLWALNGFIQGAGWPPCGRSIGHWFSVSERGTIFSVW
                     NIAHNVGGGVAGMLAAYCAKHFGLHNAFFIPGAIAILCAIYLFFRLRDTPQSVGLPPV
                     EVYKNDYPSQTHEHVEKELSTKTLFVDYILKNKLLWVVAIANFFVYIARYSMLDWGPT
                     FLREIKGANITQGGFSIMVLEFAGIPSTILFGWISDKLQGRRGMVSMLCMIPVVLAFF
                     AIVVYPQPSLWFYYAMLSVIGFFVYPPVMLLAVMGLDLTSKKAVGTAHGFISVFGYLG
                     RTAQAKGFGFILDYFATTYDIVTRWNIVLYTIVACTVISIVLLAFTWKVKPQ"
     gene            6747..8084
                     /locus_tag="PLX22_07675"
     CDS             6747..8084
                     /locus_tag="PLX22_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19818.1"
                     /translation="MNLLSLFAPPPAKPQLPEEKVKKLYPRLRWHILQATFIGYATFY
                     IVRNNLSTVARDMQTAFGYDHSQIGSILAISALLYGIGKFLLGSVSDRSNVRVFMPVG
                     LILTALCNFAFGATQSYAIHFWLWALNGFIQGAGWPPCGRSIGHWFSVSERGTIFSVW
                     NIAHNVGGGVAGMLAAYCAKHFGLHNAFFIPGAIAILCAIYLFFRLRDTPQSVGLPPV
                     EVYKNDYPSQTHEHVEKELSTKTLFVDYILKNKLLWVVAIANFFVYIARYSMLDWGPT
                     FLREIKGANITQGGFSIMVLEFAGIPSTILFGWISDKLQGRRGMVSMLCMIPVVLAFF
                     AIVVYPQPSLWFYYAMLSVIGFFVYPPVMLLAVMGLDLTSKKAVGTAHGFISVFGYLG
                     RTVQAKGFGFILDYFATTYDIVTRWNIVLYSIVACTVISIVLLAFTWKVKPRG"
     gene            8094..>8732
                     /locus_tag="PLX22_07680"
     CDS             8094..>8732
                     /locus_tag="PLX22_07680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT19819.1"
                     /translation="MHNIITVTLRLLILATPLLLNDCYLPLIPKDATKLNLILDIIIY
                     IGWQTSLLYVAYDAGWFTWERLGINVKQPKQWLWGVVLFIAIFVAYAILTIALEYCKK
                     HYGFTLASFWYFPLPGWKPLYVFLYALYLSITAGIYEEIIYRGIAIHQLRLITSNKWI
                     LIIASTLLFVAIHWSMGPGTWLEAALWGALWAYLYIQTNSLLPIMVAHFLYDL"
BASE COUNT         2590 a         1757 c         1685 g         2700 t
ORIGIN      
        1 tatttgtcaa cctatttttc cccactacca ttaccattat tatactgctc tgcaatcaaa
       61 aagtcaatat gtactcatgg tgattgagcg cagaatgtat tgacacaata ggtaaacttt
      121 tataccatta tcatcattac cgataactat cggacaaaaa aaggaggcaa cccatgaaat
      181 caatgactga ataccttacc ttcaatacca ggacaaaacg tgattacata aacatcacca
      241 ccatggttga ggatgtggtt acaagaagcg gcatcaaaga ggggttgtgc cttgtcaatg
      301 ccatgcacat cacagcctcg gtacacatta acgatgatga aaaagggctt atccatgaca
      361 ttgatgattt tttagaaaca ctggcaccgc atgaaccaat cagcaaatat cgccacaacc
      421 gtactggcga ggacaacggc gatgctcaca taaaacgaag catcatggga agagaggttg
      481 ttgtagcagt gaccaacggc aaattggatt ttggcccatg ggagcagatt ttttatgctg
      541 agtttgacgg gcagcgcccc aaacgtgtgc tgataaaagt gattggcgaa tgacatgcga
      601 cttgttgacg tacattgtca tctggaaagt cagcatttca gcggccggct tgatgcaatc
      661 attgatgcag ccagaaaggt tggtgttatc gcttttatta cctgtgcgat agttcctgat
      721 gagtgggagc tatcgcaaac aattagtcat agctttcatg aggtttactt tgccgctggt
      781 gtacacccgt ggtatatcaa aaaccaatac tttgaagaat tgcaaaagct gtatgcgata
      841 accgacagca aatacattgc cattggcgag attgggcttg atgcaaaaac cgacatggac
      901 cttgctgtgc aaaaaaaatt ttttgaagaa cagcttgcta ttgccaatga tttaaacaag
      961 cccgtcattg ttcattgcag aggagctttc aatgagctta ttgcttcact gaagcgtatt
     1021 gggaaaataa acgcaggcgg cgttatccat tcatattcgg ggaatgctga tatagcccgg
     1081 cagcttatcc gttatggttt ttacttttcg tttggaggag cactaaccta ccgcaacagc
     1141 aaaaaaaaat tgcaggtttt acaccatata tatcctgacc atgtattact ggaaaccgac
     1201 agccctgata taccccctgt ggaaaaaagt ggacagataa acctgccgga aaatattatc
     1261 tatgtggtaa aagctatgag tgagcttatt ggtaacaatg aagaagagat agcagaagct
     1321 acaacacaaa acgcaaaaaa attgttgaat ttgaacatat aattttttta tcataccaaa
     1381 taatatttag catctaaaaa atataaactc tgggaggaag caatggattt tgaaactact
     1441 acctgtatat cgtgtaaaca cttagaaatt ctagatagtt actgtaaaaa gttagatgtg
     1501 tcgctgagga catgtattgt atatatgata ctgtatgctg caaaaaagga aaaaaagaaa
     1561 gctattgcat ttaaaagaat ctgctataga aaacgggata aggacaatcc atggaaaaga
     1621 gtacatctgg tgttatatca gtcggaatat gaattttgcc tggatgtgaa gaagctatgg
     1681 aagatgtcgc ttgccaacat tattgcgttt tgcgtagaaa atgtgctggt tgaatttttt
     1741 gaatatttca gcaggcgatt aaaagagatt aattccgata actatcctta taatctaacc
     1801 agttattatg aaaacaggtc gtatacattt gattttcata aagaaaaagg catacattgc
     1861 ctgaagttct actggggacc accaccagaa gttatagaat ttaataaaaa acaaaaaaaa
     1921 ttgctttaaa atagcaatac aaaccgctat aattttattt ttataaaaac actaatgaat
     1981 atatatcatc gctgcagaat acagcgggca agtaccttgc ttttatataa cattattttt
     2041 aaggaataaa tactgcacta aaattaaaaa aattatatta caatgcaggt tttttaacta
     2101 tgaatattaa tcaaaattat tatgagcgat ttagccgtac ttcactttta ataggcaaag
     2161 aaaaccttga aaagctattc acagcacgag tttttattgc cggacttggt ggcgttggtt
     2221 catatgcctt tgaagcactg gttcgcgctg gggtgggtac tgttcatatt attgattttg
     2281 atactgtaga gatatccaat tgcaaccggc agcttttagc tttaggcagt acaataggaa
     2341 ttccaaaggt tgacgttgcc gaaaaaagag caaaagatat caaccctgat ggtatgatta
     2401 ataaaaataa aatccgtata acacctgata atgttcacgg attaatccct cacgacattg
     2461 attttgctgt tgatgccatt gatgacgttg atgcaaaaac atcacttatt acaacattgc
     2521 tggggaggca tattcctttc atttcatgta tgggtgcggc taatgcatgt aatccggaaa
     2581 gtttacgagt tagcgacatt agtaaaacac agtactgtcc gctggcacgc accatgcgaa
     2641 agcgtctcaa aaatattggg attgctggag gatttatgtg cgtttatttc cacgaggaac
     2701 gtacaggcag tacaaccaat gttagcacaa acaatacaag gggaagttta tcgtatatgc
     2761 cgggtataat gggacttgcc gctgctggct atgcaattaa ttttttaata gggagatagc
     2821 ttcaccggta actatcgcca aacatttttt aatgatttgc tgttataccc agcgatgata
     2881 aaagcactac aacctcgtaa gctgataaat cggtatacat aacaggaatg tcctcctcat
     2941 cagcacgttt gacaaattca tcacggggtt ttattccacc tgccactaca atagcgctaa
     3001 aatcttttaa tgatgcaact gccacagcat tgggatgcgt taaaatggta atccagatat
     3061 ttcctttttt accgttagcg ttaacatccg aaagcatatc gccgacataa ccaccagtaa
     3121 tgccttttgt gcaatcagct tttgtggtta acagttcaaa atttgttttt tgctggattt
     3181 cgtttacagt gatgtccata atcaaatctc cataatggtt gcttgatgtt atatctatgc
     3241 atatctttgt accttcacct ggtctggagc aaatgcgcat aacattagca ttgcgtttaa
     3301 tattcgacaa acccatacct gcaccaaatc caagcctgcg tatttcatcg gttgctgttg
     3361 aaaaaccctc ctgtactgcc agcctgatat tttctatccc ttttcctttg tcctttacca
     3421 ccagatgtac tgaatttggt ctgcaatatg caaaaaaata tccaacggtg gtatacgaaa
     3481 caacatttat ctccgcttca tataatacta ttgctaccct ttgaatcata tgggaaggat
     3541 attcccgcgt tctcaactca cgcttgagcg aagtcgaaat cctcccggca ttatcaaaat
     3601 caccaccttt caatgtatat gaaaatatat gatttgcaca ctgcatgcgc taaacttttt
     3661 aatcaacttt ttcaatagaa gctttcatac ctgcaccata aagtcttccg caggtttcaa
     3721 acattataca ttttgttctc agcaatggta tatttttacg ttttgccagc tctaccgtat
     3781 ccatctgtgg aactttaccc cgtacaaata ccacaccaat aagatcaagg acttccaccg
     3841 tacgcaccac ctgcggattg gtaaggccgg taataaacaa tgcacgctct tttgcatatg
     3901 ccagcacatc acttaacaaa tctgatgcaa aagctgtttc tacatctaca gtattgagca
     3961 tttcttctcc cactatgaca tcagcatcaa gaattttttt aatctcggac aatttcatat
     4021 tatttctacg ttttccttat tcagaagtta tagtacaata cacccatttt acattttttc
     4081 atacaattat acatatcact ttttagtcaa gtaataaata tcatcctttg accatccctc
     4141 taaaatagat atcggcggct gtcataatag ctttttctaa atctaatgat tcatcattaa
     4201 ccgaccactg aaaaaccaat tctttgatgg accctacaat acacatggac gtgaaataag
     4261 gatcaagcat gggaaacacc ctcccctttc cctgagcctt tgcaatataa tctgcagaca
     4321 tggcaataat tttattataa aaatcattaa gtttttgtaa aagtgtttta tccaatccca
     4381 ttgcttcttt taacataata cgtgcaaacg ttttaatttt tggatcgcga ataaggtatt
     4441 gggcgatagt tctatagata ttaattactt cggttaatgg tttatccatt gaaatatcaa
     4501 gtacacttaa aatacggtac atctcttcca gttgactatc tactatcata tgtaataagt
     4561 catctttacc attgaagtga agataaaatg tccctttagc tatatcagct tttttcagaa
     4621 tatcttccaa ttttgtattg tgataccctt tttttgaaaa tagttcaagg gcaatcatca
     4681 ttatttctga ttgtcgtttt aatccatcac tgcgtttttt tgtaatcttc atcactccct
     4741 caataataca aattgagata cattgatatc aatactaata aatacaggct aagaaatttc
     4801 aaatacattt ttattataac ataccacatt tactatatca agtatctgaa attgcaatca
     4861 agaggtcatc cttaatcgta atccagcatc tcaattaaag aatagattct tcatttgcaa
     4921 ccgctccctc agaatgacac aaagtactgc atctcacaga atgacaatgt gaactgtgtc
     4981 attttgaaac gtagaaactc atcctgaacg ctgattcaga atctaacgtt gaacatctgg
     5041 gttccgggtc aaacccggaa tgacaatttt gcaggttccc ataataaaaa agatttgcaa
     5101 atacacatat atcatgtact catttactgt atcatacttt ttaccttaag ggatgtcatt
     5161 atgaacctgc tatcactctt tgcaccgcca ccggcaaaac cacaactgcc agaagaaaag
     5221 gtgaaaaaac tttacccgcg attgcgatgg cacatcctgc aagcaacatt catcgggtat
     5281 gcaaccttct atatagtcag aaataatctt tccactgttg ccagagatat gcaaacagcg
     5341 tttggctatg accattcaca gattgggagc atactggcaa taagtgcgtt actgtacggg
     5401 attggcaaat ttttgcttgg ctctgtctcg gaccgcagca atgtgcgtgt tttcatgccg
     5461 gttgggctta tactaaccgc tttatgtaat tttgcatttg gtgcaacgca gagttatgcc
     5521 attcacttct ggctatgggc tttgaatgga tttattcagg gtgcaggctg gcccccgtgc
     5581 ggcaggtcca ttggtcactg gtttagcgtc agtgagcgcg gcaccatctt ctcagtatgg
     5641 aatattgcac ataatgttgg cggaggggtt gccggtatgc ttgctgccta ttgtgcaaaa
     5701 cactttggtc tgcacaatgc tttttttatc ccgggtgcta tcgccatact gtgtgccata
     5761 tatctttttt tcagactgcg cgatacaccg caatcggttg gtctgccacc tgttgaagta
     5821 tacaaaaacg attacccttc tcaaacacat gaacacgttg aaaaggaact atcgacaaaa
     5881 actctttttg tagattatat actcaaaaac aaattgctct gggtagttgc cattgcaaac
     5941 ttctttgtct atattgcccg ctacagcatg cttgactggg gtccgacatt tttacgtgag
     6001 ataaagggag caaacattac acagggtgga ttcagcatta tggtgcttga gtttgcaggc
     6061 atcccatcaa caattttgtt tggctggatt tcggacaaat tgcagggacg gcgcggcatg
     6121 gtaagtatgc tatgtatgat accggtagtg ctggcatttt ttgcaatagt ggtatacccg
     6181 caaccatccc tgtggtttta ctacgcaatg ctttcggtca ttggattttt tgtgtatccg
     6241 ccggttatgt tgttagcagt tatggggctt gatttaacaa gcaaaaaggc tgtaggtact
     6301 gcacatggct ttatcagtgt gtttggatat cttggcagga cagctcaggc aaaaggattt
     6361 ggctttattc ttgattactt tgccacaaca tacgatatcg ttacccgatg gaacattgtg
     6421 ctctacacca tcgttgcatg cactgttata tcaattgtgc tgttagcatt tacatggaag
     6481 gttaaaccac aataataata tgaaaatatc tggcgatagt catcctgaat ttacagactg
     6541 tataaaagac atatcttttc ataaaaataa taaaaaatat cttctacttt gctattatca
     6601 atatagcttc atttaaaatt attttgattc agggtcaaac ccggaataac aattttgcag
     6661 gttttcataa taaaaaagat ttgcaaatac gcatatatca tgtactcatt tactgcatca
     6721 cactttttac tttaagggat gtcattatga acctgctatc actctttgca ccgccaccgg
     6781 caaaaccaca actgccagaa gaaaaggtga aaaaacttta cccgcgattg cgatggcaca
     6841 tcctgcaagc aacattcatc gggtatgcaa ccttctatat agtcagaaat aatctttcca
     6901 ctgttgccag agatatgcaa acagcgtttg gctatgacca ttcacagatt gggagcatac
     6961 tggcaataag tgcgttactg tacgggattg gcaaattttt gcttggctct gtctcggacc
     7021 gcagcaatgt gcgtgttttc atgccggttg ggcttatact aaccgcttta tgtaattttg
     7081 catttggtgc aacgcagagt tatgccattc acttctggct atgggctttg aatggattta
     7141 ttcagggtgc aggctggccc ccgtgcggca ggtccattgg tcactggttt agcgtcagtg
     7201 agcgcggcac catcttctca gtatggaata ttgcacataa tgttggcgga ggggttgccg
     7261 gtatgcttgc tgcctattgt gcaaaacact ttggtctgca caatgctttt tttatcccgg
     7321 gtgctatcgc catactgtgt gccatatatc tttttttcag actgcgcgat acaccgcaat
     7381 cggttggtct gccacctgtt gaagtataca aaaacgatta cccttctcaa acacatgaac
     7441 acgttgaaaa ggaactatcg acaaaaactc tttttgtaga ttatatactc aaaaacaaat
     7501 tgctctgggt agttgccatt gcaaacttct ttgtctatat tgcccgctac agcatgcttg
     7561 actggggtcc gacattttta cgtgagataa agggagcaaa cattacacag ggtggattca
     7621 gcattatggt gcttgagttt gcaggcatcc catcaacaat tttgtttggc tggatttcgg
     7681 acaaattgca gggacggcgc ggcatggtaa gtatgctatg tatgataccg gtagtgctgg
     7741 cattttttgc aatagtggta tacccgcaac catccctgtg gttttactac gcaatgcttt
     7801 cggtcattgg attttttgtg tatccgccgg ttatgttgtt agcagttatg gggcttgatt
     7861 taacaagcaa aaaggctgta ggtactgcac atggctttat cagtgtgttt ggatatcttg
     7921 gcaggacagt tcaggcaaaa ggctttggct ttattctgga ttactttgcc acaacatacg
     7981 atattgttac ccgatggaac attgtgctct actccatcgt tgcatgcact gttatctcta
     8041 ttgtgctgtt agcatttacc tggaaggtta aaccaagggg ataagatttt tttatgcaca
     8101 atataataac cgttacactg cgacttctaa ttttagcaac accactattg ctgaatgatt
     8161 gctacctgcc tcttattccg aaagatgcaa caaagcttaa tcttatcctt gacatcatca
     8221 tctacattgg ctggcaaacc tcgcttttat atgttgcata tgatgccggg tggtttacat
     8281 gggaaagatt gggcatcaat gtgaaacagc ccaaacaatg gctatggggt gtagtacttt
     8341 ttattgctat atttgtagcg tatgccatac taactatcgc actggaatat tgtaaaaaac
     8401 actatggatt tacccttgca tcgttctggt atttcccatt gccagggtgg aaacctttgt
     8461 atgttttttt gtatgcgctg tacctttcaa tcaccgcagg catctatgaa gagataatct
     8521 accgtggcat tgcaatacat cagcttcgtc ttataacatc aaataaatgg atactcatca
     8581 ttgcatcaac gcttcttttt gtagccattc actggtcaat gggtcccggc acatggcttg
     8641 aagcagcatt gtggggagca ctatgggcat acctgtatat acaaacaaac tctttgctgc
     8701 caataatggt tgctcatttt ctttatgacc tg
//
LOCUS       DAOSOX010000093         8720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8045_length_8720_cov_7.685747, whole genome shotgun sequence.
ACCESSION   DAOSOX010000093 DAOSOX010000000
VERSION     DAOSOX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_8720_cov_7.685747"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..480
                     /locus_tag="PLX22_07685"
     CDS             283..480
                     /locus_tag="PLX22_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19820.1"
                     /translation="MAMPGLPEILIILMLLLILVWIVVVIKIIISSLSASRKVAWILC
                     SIVVLFIAIQFILFVMGILMR"
     gene            601..993
                     /locus_tag="PLX22_07690"
     CDS             601..993
                     /locus_tag="PLX22_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT19821.1"
                     /translation="MKSIKLGKATSFGGFVASIIAIIGGIGWTLLVISLFSQRNEMEN
                     PVFYFFVLFGIIFIGVGIAIAVFSYKNLHSKNGYSLYDIVDSSEEKDAFHSSWDSTAA
                     KGTMNYCPHCGTSLRQDFIFCPGCGKKL"
     gene            993..1463
                     /locus_tag="PLX22_07695"
     CDS             993..1463
                     /locus_tag="PLX22_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19822.1"
                     /translation="MICTIVPVNLFATEYDVFCGSKHVAKFKKPALSLKSTCSITMND
                     KEYSIYREGVVSGDWILEEKHAGRVAVATKPSFLIDSFAIQYNNKAFQLKSKKLRLTN
                     QFIIYENDQQIGRIYNPSFFSRKLQCAIDGDSPLEFMIFAMYLASIIIDRRSSQ"
     gene            1652..2083
                     /locus_tag="PLX22_07700"
     CDS             1652..2083
                     /locus_tag="PLX22_07700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT19823.1"
                     /translation="MEIQNLTGLELMKSMIEGKIPPAPIAETIPMNGVDVAKGYIKFL
                     AKADKRHINPLGGVHGGFAATVLDSVTGCSVHTMLEAGAGYATIDLNIKMIKPVPLNR
                     ELIAEGKVIHLSHNIGISEGTLTDEDGKLYALATATCIIFK"
     gene            2146..2730
                     /locus_tag="PLX22_07705"
     CDS             2146..2730
                     /locus_tag="PLX22_07705"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOT19824.1"
                     /translation="MNANKTQQLLLKTGARLVHTKGFNNTGIQEILQCAGVPRGSFYF
                     YFKSKEEFGLALIEYYADMMCSMLRNYLTDASCSPIERIKLFFEGSARYYEQHKFEGG
                     CPVGNLSQEMSCLSEPMRVKLLSALRGMHSIIQQCIKEGQDAGQIDSSLDAGALAVFV
                     FNAWEGSLIDMKVSKSVYPLEICKTMVLRFIQSQ"
     gene            2755..>3794
                     /locus_tag="PLX22_07710"
     CDS             2755..>3794
                     /locus_tag="PLX22_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT19825.1"
                     /translation="MYEQLRQVLDAHPATAPKTDTIIAILKMLFTPDEAAIAMNMSFK
                     VMDSEKIASLCSLPGEEAEKLLESMANKGIIYSKNKDGKKLYGLVPLIPGVFEFPFMK
                     SDGSERFQKLGRLWEQYHDEAFGVSFCGNPTPLMRVVPVEKSLSPQNRVHPYEEVRNF
                     IEQARYIAIADCACRTSVGKCDKPKDVCIIFDSAAQFLAERGFARQVTKAEAMDALDR
                     SEKAGLVHTSNNSADRANLICNCCPCCCTVLRGRTQHNHPHAFEPSRFEAHVNDVNCT
                     GCGICVDERCPMKAITMRDGIAVVDTARCIGCGLCVTGCPETAIELRERASIPEIPAT
                     VVDMAMKVVTEKG"
     assembly_gap    3795..3894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3895..4419
                     /locus_tag="PLX22_07715"
     CDS             <3895..4419
                     /locus_tag="PLX22_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011786288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT19826.1"
                     /translation="PFGIDEDNRVSKPIVSALQGICFTVGWELMLATDVRVAASNVRL
                     AQIEVKRGIYPVGGATVRLFEEIGWGNAMRYLLTGDEMNAQEAYRLGLVQEVVEPGKQ
                     LERAIEIATVIAKQAPLGVRAILKSARLSRVEGAKAALERLLPDLLPIMKSEDAQEGV
                     RAFIERREAAFKGK"
     gene            complement(4431..4940)
                     /locus_tag="PLX22_07720"
     CDS             complement(4431..4940)
                     /locus_tag="PLX22_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOT19827.1"
                     /translation="MEIILFITALLFALGGILGSVLPILPGPPLSFFAVILLHWSGFA
                     SFSFTFFIITGIIAAILTVFDYVLPSWIVNKFSGSKQGVIGSMIGLIVGSIILPIIGI
                     IIGPFIGAFIGELVANNNALTALKAALLTFIGFLISTGLKLGYTITIAYYIIKALMGQ
                     PPAPDYPII"
     gene            complement(5025..5699)
                     /locus_tag="PLX22_07725"
     CDS             complement(5025..5699)
                     /locus_tag="PLX22_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HOT19828.1"
                     /translation="MKRVTYGLIAFIVCCSIGMLYAQDEKAKEAQKVLEQEKQLSDQI
                     YKEIVLEDFETVEYTDKNISLRVVKDEKAGVSMRTDYPAPIQNSKRYLGVKVFAKSGS
                     VLTIIPAKPLIIDKHCKYINMWVYGKNFSGELSIMVKDANGNIKRLVMGKLNFLGWRK
                     LSVPITDEIAQEDKYLAQKRNIEIVKILYNPSTLERLPAWNYFYIDDITAIVREKYID
                     KQSDEW"
     gene            complement(5840..6961)
                     /locus_tag="PLX22_07730"
     CDS             complement(5840..6961)
                     /locus_tag="PLX22_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOT19829.1"
                     /translation="MIKILKKTCILFFAMGVIMTVDVSCKQASDRPKGFQSNAIKVMT
                     INVWSGLDYIGTFKMGEYETPQIREKRYQALLKEIANLNPDIIAINEANFLPDYMKRL
                     ASDLNYDYIYHVGVAGLKIGRLGIPTNLKEGDGLLARKQLQLQQVGHKKLSGGGIITN
                     CFSFHTQDATQVLEGRVIINEKPVFVAVTHWHASPPDTPQNRILLKELKETYGYSDDE
                     YTQAIQQLEADNKWRNNESIAMAEFLKKVVPADAPCIVMGDFNTTIDSPEMRYFLSQG
                     YHDTFLPSNNTSPYTWNPMENANIKKYYVTDTRTKFDSLYAHCTKINDLQPKTIDYIL
                     TNNVIPRTMIHSSTVCCTTLHDGYHPSDHFGVFAEILIQ"
     gene            7402..>8720
                     /locus_tag="PLX22_07735"
     CDS             7402..>8720
                     /locus_tag="PLX22_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19830.1"
                     /translation="MMIVLLIAKSSVFANGSFDWFWFIYEQGTYYDSTYTVYRPLYVK
                     NVYLDSSFSASLMPLIYWNYTTPNQDKTRGFLGFYVSIDYRSANHYENDTGVFPFVMY
                     GTSNDKEDQYFHLWPFGGTVKSKLGADAITTYAFPGFVLFILYPTTSYYVLAVYLVAS
                     MIPIYAHYTIRDYDGHAIIWPLIRWGKSPQRDDFRIMPFYAHSKKKGYYSTASYGMLY
                     NVREEYIGDDTMHTVFLVPFYARRWLDSGKADSSTLLWPFFSWGYNDKHGNVEINFPW
                     PLVQIRDCDDPYIYKRIFFPFYGKYIEKRKTTFFISPFYFVLEKQKDSFHSQYSFYGI
                     LFWHFQRDYKYGHKRYGNHWRFYKLWPLFHYEYNDKGDSSFAILSIFPFRDEEGYERL
                     YQPFFSLVEYATRDDGSKRFGLLLRTYFQSWGDSYFKMKIPFIISYH"
BASE COUNT         2531 a         1544 c         1817 g         2728 t
ORIGIN      
        1 ggcatgatgg ttttgttcaa tctgtagtgt atagccccga tgggcggtat attgtttcag
       61 gaggttttat aaccatcaag gtatgggata tgcaggtaat tttgccaaaa gttttttaaa
      121 taggtaatat ttgttttggg atttattacc atatcggcag ccatgcattg aagtatatat
      181 gtatggtgat agttatatta aatgtatgat taatgcttca tgttttttat ggttatataa
      241 tatcatggat gtacacaata tctctataag ggggggtagt gtatggcaat gccaggttta
      301 cctgaaatat taattatttt aatgttactg ttgatactgg tatggatagt tgttgtgata
      361 aaaattatca ttagttcctt atccgcatca agaaaggttg catggatact gtgttctatt
      421 gtagtattat ttattgctat tcaatttata ttgtttgtga tgggcattct catgagataa
      481 acggtattgt aatgatttcc tcaagttaat aactgttgtt gcatcaatat cataatcata
      541 atatttttta tatataatca ggctgtataa aaacaagcaa ttgagaacaa ggaggatact
      601 atgaaaagta ttaaacttgg gaaggcaaca tcatttggag gatttgtagc ttctattata
      661 gcaattatag gtggaattgg atggacactg ctggttatat cattgttttc acagagaaat
      721 gagatggaaa atccagtgtt ttattttttt gttttgtttg gaattatttt tattggtgtt
      781 ggtattgcaa tcgctgtttt tagttataaa aatctccaca gcaaaaatgg atactcatta
      841 tatgatattg ttgacagctc cgaagagaag gatgcattcc actcatcatg ggacagcaca
      901 gccgctaaag gtactatgaa ctattgtcct cactgcggta cttcgctgcg gcaggatttt
      961 atcttttgtc ccggttgtgg aaaaaaatta taatgatctg tacgatagtt cctgttaatc
     1021 tatttgcaac agaatatgat gtcttctgcg gcagcaagca tgttgcaaag tttaaaaagc
     1081 cagccctttc tctaaaaagt acatgcagta taacaatgaa cgataaagaa tattctatct
     1141 atcgtgaagg tgttgtttca ggagattgga tactggagga aaagcatgct ggcagggttg
     1201 ccgttgcaac aaaacccagc tttttaattg attcctttgc aatacagtat aacaataaag
     1261 cctttcaatt aaaatcaaaa aagctacgtc ttaccaatca gtttattata tatgaaaacg
     1321 atcagcagat aggaagaatt tataatccat catttttttc gcgaaagctt caatgcgcta
     1381 ttgatggtga tagtcccctt gaattcatga tatttgctat gtatctggca agtatcatca
     1441 tagaccgcag aagttcacaa taacaatagc aagctgtata tactataacc ctgtgtattg
     1501 cagattgaga aatggtgtac aacaaaaaat aaaaaaatgt gttgacagta gtagaccggt
     1561 ctattactat aatataatgt tcctgtgtat aaagctttta agttttacat ggttatacct
     1621 tacaatttgt ttacgtttat ggaagggata tatggaaatt caaaatttaa ccggacttga
     1681 attgatgaag agtatgattg agggcaaaat tccaccggca cctattgctg aaactatccc
     1741 tatgaatggc gtagacgttg caaagggtta cataaaattt ttggcaaaag ctgataaacg
     1801 gcatatcaat cctctgggcg gcgttcacgg tggatttgct gcaacggtgc ttgactcggt
     1861 taccggctgt tctgttcata cgatgctgga ggcgggtgct ggctatgcaa ccattgatct
     1921 taacattaaa atgataaaac cggtgccgct taacagggaa ctcattgctg aaggtaaggt
     1981 gattcatcta tcacataaca ttggaatttc agaaggcacc cttacggatg aagatggaaa
     2041 gctgtatgct cttgcaacgg caacatgcat catcttcaag taactatcga acatacatta
     2101 tttgttggct tttagttaac ttgttaccat gatgattgaa atagtatgaa tgcaaacaaa
     2161 acacaacagt tgctgcttaa aacaggtgct cgtcttgtac acacaaaggg tttcaataat
     2221 accggcattc aggaaatttt acagtgtgct ggtgttccca gaggctcttt ttatttttat
     2281 ttcaaaagta aggaagaatt tgggttggcg cttattgagt attatgcaga tatgatgtgc
     2341 agcatgttgc gtaactactt aaccgatgca agctgtagtc ctatagaaag gattaagctt
     2401 ttttttgaag ggagtgcccg ttactatgag caacataagt ttgaaggtgg ttgtcctgtt
     2461 ggtaaccttt cgcaggagat gagctgcctt agcgagccaa tgcgtgtaaa gttactttct
     2521 gcattgcgag gtatgcacag tattatacag cagtgtatca aagaaggaca ggacgctggt
     2581 cagattgata gcagtcttga tgcgggagca ctggcagttt ttgtttttaa cgcatgggag
     2641 ggctcgctta ttgatatgaa ggtttcaaaa agtgtttatc cacttgagat atgcaaaacc
     2701 atggtgttgc gttttataca atcacaataa tacaactgga ggctttcaat ggaaatgtat
     2761 gaacaacttc gacaggtact tgatgctcat cctgccactg caccaaagac agataccatc
     2821 atcgcaatac tcaaaatgct ttttactccc gatgaagctg caattgcaat gaatatgagc
     2881 tttaaagtca tggatagcga aaagattgca tcgctgtgtt cgttgcccgg agaggaagct
     2941 gaaaagcttc ttgaatcaat ggctaacaag ggtatcatct attcaaaaaa caaggatggg
     3001 aagaaactgt atggattggt accgcttatc cccggtgttt ttgaattccc atttatgaaa
     3061 tctgacggtt cggaaaggtt tcaaaagctt ggcaggctat gggaacagta tcatgatgaa
     3121 gcctttggtg tttcgttttg tggcaatcca actcctctca tgagggttgt acctgttgaa
     3181 aaatcattaa gtcctcaaaa ccgtgtacat ccttacgagg aggtaagaaa ttttattgaa
     3241 caggcgcgct acattgccat tgcagactgt gcatgcagaa ccagcgtggg taaatgtgat
     3301 aagccgaagg atgtgtgtat tatttttgat agcgccgccc agtttttagc cgaaagggga
     3361 tttgctcgtc aggtaacaaa agctgaagca atggatgctc tggataggtc tgaaaaagca
     3421 ggattggtcc ataccagcaa caatagtgct gaccgtgcca accttatttg caactgttgt
     3481 ccgtgctgct gtacggtttt acgtgggcgg acacagcata atcatcccca tgcgtttgag
     3541 ccaagccgat ttgaagcgca tgtcaatgac gtcaattgca ccggctgcgg tatatgtgtt
     3601 gacgagcgtt gccctatgaa agctattacc atgcgtgatg gtatagccgt agttgatact
     3661 gccaggtgca ttggatgcgg attgtgtgtt accggatgtc ccgaaacagc aattgaactt
     3721 cgtgaacgtg catctatccc tgaaataccg gctaccgttg ttgatatggc aatgaaggtt
     3781 gttacggaaa aaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccttt
     3901 ggtatagatg aagacaacag ggtgtcaaaa ccaatagtgt ctgccctgca gggtatatgc
     3961 tttacggttg gctgggagct aatgcttgct accgatgtgc gtgttgctgc cagcaatgta
     4021 cgattggcgc agattgaggt gaaacggggc atttatcctg ttggtggtgc aacggtgagg
     4081 ctttttgaag agataggctg gggcaatgct atgcggtatt tgctaactgg tgatgagatg
     4141 aatgcacagg aagcctaccg tttaggattg gtacaggaag ttgttgaacc tggcaagcag
     4201 ctggagcgag ctattgaaat tgctactgtt atagcaaaac aggctccatt aggggtcaga
     4261 gccattttaa aatcagctcg tttaagcaga gttgaaggcg caaaggcggc tttagaacgg
     4321 ttactgcctg atttgctgcc tatcatgaaa agcgaagatg cgcaggaagg ggtcagagcc
     4381 tttattgaaa ggcgtgaagc tgcttttaaa ggcaaataaa cagtaaatag ttatatgatg
     4441 gggtaatcgg gtgctggagg ttgccccatt aatgctttaa taatgtaata ggctatggta
     4501 atggtatacc ccagcttcaa gccggtactt attaaaaagc caataaatgt aagcaacgct
     4561 gcttttaatg ctgtcagtgc attattgttt gcaaccagtt cgcctataaa tgcaccaata
     4621 aatggaccaa taattatacc aataataggt agtataatac ttccaactat taaccctatc
     4681 attgaaccaa taacaccctg tttggaacca ctaaacttat tgacaatcca tgaggggagt
     4741 acataatcaa agaccgtcag gatagcggcg ataattcccg taataataaa aaatgtaaaa
     4801 ctaaaagagg caaaaccgct ccaatgaaga aggataactg caaaaaaact taaaggagga
     4861 ccgggcagta taggaagtac cgaacccaga atacctccta atgcaaaaag caatgctgta
     4921 atgaataata ttatttccat aataagtaat ataccttcat tgttctttga taaaatacag
     4981 aagggcacat gccccatgtg ccctgaactt acagtgtata atttttacca ctcgtcgctt
     5041 tgcttatcaa tatatttttc gcggacaatt gctgtaatat catctatgta aaagtaattc
     5101 catgcgggaa gccgttccaa tgtgctggga ttatacagta ttttaacaat ctctatattc
     5161 cgcttctggg ctagatattt atcttcctga gctatctcgt cggtaattgg aacggaaagc
     5221 tttcgccatc ctaaaaagtt aagcttaccc attaccagcc gtttaatatt cccattggca
     5281 tctttaacca tgatagatag ttctcctgaa aaatttttcc catacaccca catgtttata
     5341 tatttgcaat gcttatcgat gataaggggt tttgccggaa taatggtcaa taccgaaccg
     5401 cttttggcaa aaaccttaac acccagatac cgttttgaat tttgtatcgg cgcaggataa
     5461 tcggtacgca ttgaaacgcc tgctttttca tcttttacaa ccctgaggct aatattctta
     5521 tctgtatatt ctacagtttc aaaatcctca agtactattt ctttatatat ctggtcgctg
     5581 agttgctttt cctgttctaa aactttctgc gcttcttttg ctttttcatc ctgtgcatag
     5641 agcatcccta tagaacaaca tacaatgaaa gctatcaatc catatgtaac tcgcttcata
     5701 ttaataaaaa cctccttgaa aaatttataa atacctgtca cctttgccgg ttgtaacaca
     5761 tacagtagct tcattataga cactactgtc atggaatgtc aatagaaaat ataattatat
     5821 caacctaaaa gtataaattt tattgtatta atatctctgc aaatactcca aaatggtcgg
     5881 atggatgata tccgtcatgg agtgtagtgc agcatactgt tgaactatgg atcatggtac
     5941 gtgggattac attattagtc agaatatagt caattgtttt tggctgtaga tcattgattt
     6001 ttgtgcaatg agcatagagc gaatcaaact ttgtcctggt gtcagttaca taatactttt
     6061 tgatattggc attctccata ggattccatg tgtacgggct ggtattgttt gaaggcaaaa
     6121 atgtatcgtg atagccctgt gaaagaaaat agcgcatttc gggtgaatca atcgtagtgt
     6181 taaaatcacc catgacaata cagggcgcat cagcggggac aacctttttt aaaaattcag
     6241 ccatagctat tgattcattg ttacgccatt tattgtcagc ttcaagctgt tgaatagcct
     6301 gtgtatattc gtcatccgaa taaccatatg tttcttttaa ctctttcagc agtatcctgt
     6361 tttgcggtgt gtcaggtggc gaggcatgcc agtgagtaac agctacaaaa acaggttttt
     6421 cattgataat aactctgccc tccagaacct gtgttgcatc ctgcgtatgg aatgaaaagc
     6481 agtttgttat gatgccacca cccgaaagct ttttatgacc aacctgttgc aactgcaatt
     6541 gttttcgtgc cagcaatccg tcgccttctt ttaaattggt gggaataccc aatcttccga
     6601 tctttagtcc ggcaacacca acatggtaga tataatcata gttaaggtca ctggcaagcc
     6661 gtttcatata atcgggtaaa aagttagcct cgttaattgc aatgatatct ggattcagat
     6721 tggcaatctc ttttagtaag gcttgatagc gtttttcacg aatttgcggt gtttcgtatt
     6781 cccccatttt aaatgttcca atatagtcca atcctgacca cacgttgatg gtcattactt
     6841 ttattgcatt ggattgaaat ccttttggcc tatccgatgc ctgtttacac gatacatcaa
     6901 cagtcataat aacccccata gcaaaaaaaa gtatgcatgt tttttttaaa atctttatca
     6961 tggcctcctc caatcaaatt atttttttgt ttgtgcgaaa gttactgtac aataagtcaa
     7021 tatctctcca ttgacatggc actggcatca tggttgcctg gatgttttgt gaatatgata
     7081 tacttttcaa agcatataca attgcacata tgtatacggt gtcaaccatt ttacggttgt
     7141 atcaatccat aggaactatc gcccatgctt ttgtaacaaa atagttgatt gtaattttaa
     7201 aagtgataat ttagttacta tacttttttt atttttcgtt gaattaatca ttgcaaccaa
     7261 ttgatgcttt atccagtata acattacata gcaatgcaaa ggaatagcgg gcgcgaagaa
     7321 aattaaatga aatgcagaca actaaacata gcaacaatgc aatccagtaa atgtttgaaa
     7381 aggtttttgc aaacatgttg tatgatgatt gtactgttaa ttgcaaaatc gtcggtgttt
     7441 gctaacgggt cgtttgactg gttctggttt atctatgagc agggtacata ctacgatagt
     7501 acctatacgg tataccgccc tttgtatgtg aaaaatgtgt acctcgactc ctcgttttct
     7561 gcctcgctta tgccgttgat atactggaac tatacaacgc ccaatcagga taaaacaaga
     7621 ggttttcttg gattttatgt atccatagat taccggtcag caaatcatta tgaaaatgac
     7681 accggtgtgt tcccctttgt aatgtatggc acttcaaatg acaaagaaga ccagtatttt
     7741 catctatggc cgtttggtgg gacggttaaa agtaaactgg gagctgatgc aataaccacc
     7801 tatgctttcc cggggtttgt actttttata ctgtatccta ctacatcgta ttatgtactt
     7861 gctgtttatc tggtggcttc aatgattccg atttatgctc attacaccat cagggattat
     7921 gatggacatg ctatcatctg gccattgata cggtggggta aaagtcccca acgtgatgat
     7981 ttccgtatca tgccgtttta tgctcatagc aaaaaaaaag gatactattc aactgcatcg
     8041 tatggaatgc tctataatgt tcgtgaagaa tatattggcg atgataccat gcatacggtg
     8101 tttctggtgc cgttttatgc acggcggtgg ttggattccg gcaaagctga cagctctacg
     8161 ctgttatggc catttttttc atgggggtat aacgataagc atggcaatgt tgagataaat
     8221 tttccatggc cgttggtgca gatacgggac tgtgatgacc cctatatcta taaaagaatc
     8281 tttttcccat tttatggaaa atatatagaa aaacgtaaaa ctaccttttt tatatccccg
     8341 ttttattttg tgcttgaaaa acaaaaggat agcttccatt ctcaatattc gttttatggg
     8401 atattgttct ggcattttca gcgtgattat aagtatggac ataagcggta tgggaatcac
     8461 tggcgctttt ataaattatg gccattgttt cactatgagt acaatgataa aggcgacagc
     8521 tcgtttgcaa tcctttcaat attcccattc agggatgaag agggatatga gcgattgtat
     8581 cagccttttt ttagtttggt tgagtatgct acccgtgatg atggtagcaa gcgctttgga
     8641 ttattactgc gaacgtactt ccagagctgg ggcgatagtt acttcaaaat gaagattccg
     8701 ttcatcattt catatcacag
//
LOCUS       DAOSOX010000094         8549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8215_length_8549_cov_7.659524, whole genome shotgun sequence.
ACCESSION   DAOSOX010000094 DAOSOX010000000
VERSION     DAOSOX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8215_length_8549_cov_7.659524"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLX22_07740"
     CDS             <1..271
                     /locus_tag="PLX22_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008485833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6363 domain-containing protein"
                     /protein_id="HOT19831.1"
                     /translation="NNSKGTGILNLLFMNYPEFGKAFMQRASTYNAAIDFIENPPDDC
                     RIVEVCPPATFATKRTTTNKEILLKDYQVAINIGNAFCIFYKSLQ"
     gene            288..515
                     /locus_tag="PLX22_07745"
     CDS             288..515
                     /locus_tag="PLX22_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19832.1"
                     /translation="MECIWEKNYDFWYNPIHFQRMTIQDMLHCSAAVTPDKVATDFYG
                     TKITYYGLRKMSITFTQSLVNIGIKNVIESR"
     gene            complement(521..1858)
                     /locus_tag="PLX22_07750"
     CDS             complement(521..1858)
                     /locus_tag="PLX22_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT19833.1"
                     /translation="MKSNSHYVHESKLLNNIFRSLHYRNYRLFFAGQGISLIGTWIQR
                     IAMPWLVYHLTGSAFLLGLVGFAGQIPTFLLAPFAGVLTDRWNRYYILVVTQILSMVQ
                     ALLLAFLYFIGSIEVWHVIVLNVFLGLVNAFDMPARHSFIVEMVEKKEDLGNAIALNS
                     SMVNSARLVGPAIAGILIVYAGEGTCFLINGLSYIFVVTCLLQMNVAPEKKDRQSTHI
                     FAELKEGFLYTFGLAPLRYVILLLALVSLMGTPYTVLMPVFAKEILHGGSHTFGFLMG
                     ASGLGALMGAVYLASRKSVLGLWKIIPLSAAIFGFGLIAFSLSRIFILSLLLMVAIGL
                     GMITQMASSNTILQTIADDDKRGRVMSFYSMAFMGTAPFGSILAGWLADLIGVPNTIA
                     ISGVTCIIGALIFVKKLPELKKGVRSIYVKLGIIPEISRGIQTATELTVPPQE"
     gene            2354..3022
                     /gene="nfi"
                     /locus_tag="PLX22_07755"
     CDS             2354..3022
                     /gene="nfi"
                     /locus_tag="PLX22_07755"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259813.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOT19834.1"
                     /translation="MNYHRWECSVSEAIAIQENLATTVITAGKPDTITTVAGIDIALT
                     PQNIGFCIIAVFEYPSLVLIDEVFSSDPITFPYVPGLLSFREGPLVLKAFEKLTEKPD
                     ILIFDGQGIAHPRRFGIASHIGVVLDVPAIGCAKSRLYGNYEEPGITKGDRAYLYDKN
                     NKKIGVVLRTRTNVKPIFVSPGHRVGIDEAADIIMQCTGKYRIPVPTRYAHCRVGEYK
                     RSSL"
     gene            complement(3017..3337)
                     /locus_tag="PLX22_07760"
     CDS             complement(3017..3337)
                     /locus_tag="PLX22_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT19835.1"
                     /translation="MPDKFGLSIDTINKLYTVFSKYSEIEKAIIYGSRAKGNYKNGSD
                     IDLTLIGSSDLNLTTLGKIMTGIDDLLLPYTEDLFLYNYLNDPDILDHIRRAGKIFYK
                     KNKL"
     gene            complement(3330..3746)
                     /locus_tag="PLX22_07765"
     CDS             complement(3330..3746)
                     /locus_tag="PLX22_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOT19836.1"
                     /translation="MDQQDICWKQRLSNFSKALDQLTKFIDKGQLNELEEQGLIKVFE
                     YTYELAWNVIKDYFESQGETGLTGSRDAFRMAYKRGIIDDGEIWMDMIKSRILTSHTY
                     DEKIANKISSSIMYYYYPQFRKLHDFLKKLLDKNNA"
     gene            4024..5346
                     /locus_tag="PLX22_07770"
     CDS             4024..5346
                     /locus_tag="PLX22_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT19837.1"
                     /translation="MEQTTIIIAGLGSAGFSALTTVKRINPKARVVVIDPKEKDIVHP
                     CGLPYALEGIVSYEHLSQDIYLNRMNVEKITGTVQLVDAANHKLIVTSGNNEQLVNFD
                     RAIICTGYKPLVPPIVNIKKYYNNGVYTLSSVQDLLAIKSALHGKQRALVIGAGAIGL
                     EMAYALQVNGLQVTVIEAKDVLPNALDSDMASIVADYCTQNNIALALHAAVNTIEKSN
                     NFIAITNDAQYNADIIIVACGFAPNCDWAIASSLKTSKGGAIVTDNLQVCPDVYAAGD
                     CIECWSVIDKKPLPVKLATSAYKQGVIAAENGMGGNSVYVGTAGTFVTVIGNLEIAAT
                     GFSTDVAKERGFSVIAGKISATVKPEYFPSNERLTMKVLCDIHGKVIGAQAIGHGAAS
                     RINIVSLAINKGLTIDDLLQSEMAYCPAVSEVNDILLKACEFAKRRMK"
     gene            5343..6443
                     /gene="gcvT"
                     /locus_tag="PLX22_07775"
     CDS             5343..6443
                     /gene="gcvT"
                     /locus_tag="PLX22_07775"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT19838.1"
                     /translation="MTAQRTFLYEDHIKLGARMVEFAGWELPVMYTSIIDEHMAVRQS
                     AGIFDVSHMGEIVAKGRGAKAALQRLIPTDMGRLEKGKSMYTCFLNEQGGIIDDLFIF
                     MLSDNEYYLVVNAGTTEKDVEWLTLNCKTNVAIQDVSGVTSKIDLQGPQSYNILKKII
                     KDDRLEGLQRFYFFTGTYKGEPVLVSQTGYTGEFGYELFCINNIISRLWNDILDAGKE
                     YGLKPAGLGARDTLRLESCYSLYGHELSETISPIEGGIGFVVNATSDYIAKDIIEDQK
                     KNGAPREILCLEMMDKTIPRSDYPVVYNKQDIGAITSGGFSPLLKKGIAMALVKRGLV
                     TIHDEVSVGIRGKQSPAKVVARPFYKYNGKRL"
     gene            6513..6905
                     /gene="gcvH"
                     /locus_tag="PLX22_07780"
     CDS             6513..6905
                     /gene="gcvH"
                     /locus_tag="PLX22_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT19839.1"
                     /translation="MSNIPENLKYTKTHEWIDVQDTYAGVCGITDHAQEMLTDIVFVE
                     LPEVGIEVKAGEQVAVVESVKAVSDVYAPISGRITEVNKLLEDKPELLNSDPYGEGWI
                     YKIDIKDKYELDELMDAAAYAEHVAQGE"
     gene            6908..8227
                     /gene="gcvPA"
                     /locus_tag="PLX22_07785"
     CDS             6908..8227
                     /gene="gcvPA"
                     /locus_tag="PLX22_07785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT19840.1"
                     /translation="MSFLPNSDDDILEMCSEIGVSSIEDLFCDIPASIKNPTIQLPQA
                     LSEQEALTHILHIGNANEQLCSLVGAGIYNHYVPAVVDHLVSRSEFYTAYTPYQPEVS
                     QGTLTAIFEFQTFICRLTGMDIANASMYDGATALAEAVLMAIRSTGKNRVLCSATVHP
                     HYRDVLSAYLWASDAQLAIVPYKQGLTDYEQLSHMIDDNVACVIVQSPNYFGAIEDVA
                     SIADSAHNHGAVCIATFTEALSLALLKSPGSLGADIACGEGQSFGNYPACGGPLLGIL
                     ACRSKFMRTIPGRLVGKTVDEEGRDAFVMTLQTREQHIRRQRATSNICTNEGLCALRA
                     TIYLSLVGNKLIELARINHALASYCCSRLVALGYERVFTVPFFNEFVIRVNNAKEIRN
                     RLLDNGYALGISIDTYYPELNDCLLLGVTESVSNNDIDNLVELLQTI"
     gene            8239..>8549
                     /locus_tag="PLX22_07790"
     CDS             8239..>8549
                     /locus_tag="PLX22_07790"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871381.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOT19841.1"
                     /translation="MQTIFSFHKKGKNKYSIPACDPIQVDEIPESMLRDDLTLVDVSE
                     VDVVRHYRKLSSLNFGVDNGMYPLGSCTMKYNPKINDVVAMLPQFAMQHPATIEETGQ
                     GA"
BASE COUNT         2663 a         1516 c         1806 g         2564 t
ORIGIN      
        1 aaataattct aaaggcaccg gtatcctgaa tttattgttc atgaattatc ctgaatttgg
       61 aaaagcattc atgcaaaggg cttcaacgta taatgctgca atagatttta ttgaaaatcc
      121 acctgatgat tgtcgcatag ttgaagtttg cccaccggca acttttgcaa caaagaggac
      181 aacaacaaat aaagaaatat tgttaaagga ttatcaggtg gcaatcaaca tcgggaatgc
      241 gttttgcata ttttataaat cattacaata aatgatggag gatttttatg gaatgtatat
      301 gggaaaagaa ttatgatttc tggtacaatc ccatccattt tcagaggatg acgattcagg
      361 acatgctaca ttgcagcgct gcagtaacac ctgataaagt agcaacagac ttctatggca
      421 ccaaaattac atattatggg ctacggaaga tgtctattac gtttactcaa tcactggtaa
      481 atattggaat aaaaaatgtg atagagtcaa gatgatagtt ttactcctga ggaggtacgg
      541 ttaattcagt tgcagtttga attccccttg atatttcggg aataatacca agtttgacgt
      601 atattgaccg aacacccttc ttcagttctg gaagtttttt tacaaagatg agagcaccta
      661 taatacatgt cacaccgctt attgcgatag tattgggaac acctattaaa tctgcaagcc
      721 atcctgctaa gatgctgcca aaaggtgctg tgcccatgaa tgccattgaa taaaagctca
      781 taaccctgcc tcgtttatca tcatcggcaa tggtttgtaa aatggtattg cttgatgcca
      841 tctgtgttat catcccaagt ccaatggcta ccattaatag taaagaaaga ataaatatgc
      901 gggataatga aaaagcaatg agcccaaaac caaaaatagc tgctgataaa ggaataatct
      961 tccatagccc taacacactt tttctggaag caaggtatac tgcacccatc aaagcgccaa
     1021 gccctgaagc ccccatgaga aaaccaaaag tatgtgatcc tccatgaagt atttctttgg
     1081 caaaaacggg cataagtacc gtatagggtg ttcccataag gctaaccaat gcaaggagga
     1141 gtataacata tcgtaaggga gcaagtccaa acgtatataa aaatccttct tttagttctg
     1201 cgaatatatg ggtgctttgc ctgtcttttt tttctggtgc aacattcatc tgtaataagc
     1261 atgtaacaac aaatatgtag ctcaacccat taattaaaaa gcaggtccct tcgccagcgt
     1321 aaacaatcag gataccggca atggcaggtc ccactaaccg tgcactattg accattgaag
     1381 aatttaaggc tattgcattg ccaaggtctt cttttttttc taccatttca actataaatg
     1441 agtggcgcgc aggcatgtca aaggcattaa ctaaaccaag aaatacattt aacacaataa
     1501 catgccatac ctcaatggaa cctatgaaat aaagaaatgc aaggagtaaa gcctgcacca
     1561 ttgaaagaat ctgcgtaacc accagaatgt aatagcgatt ccatctatcc gttaaaactc
     1621 cggcaaacgg agctaataaa aaagttggga tttgaccggc aaaacctacc agccccaata
     1681 aaaaagctga accggtaaga tgatatacca gccatggcat ggcaattcgt tgtatccatg
     1741 ttccaatgag tgagatgcct tgaccggcaa aaaaaagacg ataatttcta tagtgaagtg
     1801 aacgaaagat gttgtttaac aattttgatt catgaacata gtgactgttg gatttcatac
     1861 ttgttgatgc ttcctatttt tgcgattatg cgattaattg tatataacat tgtgcatgcc
     1921 tgctaaccta caacgaaagt gagcacggta gacaattatg ttgtaaacta attgataata
     1981 cgtttttatt tatctcatct tttttcatat cagtgtaccc atgttgtaca tagtacaata
     2041 ctctttgtag tcaaagattt tgtttctctt tacattaatt atagagctat tgtcgtcggt
     2101 taaccataaa ttatactgtt gtcatgtatt ttcattaaaa aattaatcat atcaaccata
     2161 gatgcaatgt tataagttta catagtatgc aatactatga gcataacact atccatgtta
     2221 ttccctgcag gtatagcagc ttgccgatgc caggtaatac catgagctat cgaacacaat
     2281 aaaaatatgc cagcatcttg acttgacaaa ttttgatgat ataactatgt aatgcatggg
     2341 ggtcagagcc tttatgaatt atcaccgctg ggaatgctcc gtttctgaag caatagccat
     2401 tcaggaaaac cttgccacaa cggtaataac agcagggaag cctgatacca ttaccactgt
     2461 tgctggtatt gatattgctc tgaccccaca aaacattggt ttttgcatca tcgcagtttt
     2521 tgaataccca tcgcttgtgc tcattgatga agtttttagc tctgacccca taacctttcc
     2581 ctatgtgccg ggcttgctgt cgttcaggga aggaccgctg gtgctgaaag catttgaaaa
     2641 gcttacggaa aaacctgata ttcttatttt tgacgggcag gggattgcac atccccggcg
     2701 ctttggcatt gcaagccata ttggtgtagt acttgatgtg cctgcaattg gctgtgcaaa
     2761 atcacggctc tatgggaatt atgaggagcc ggggattact aagggtgaca gagcatacct
     2821 gtatgacaaa aacaacaaaa aaattggtgt ggtattacga acgcgtacta acgtaaaacc
     2881 aatttttgta tcaccgggac atagagtagg gattgatgaa gcagcagata ttataatgca
     2941 gtgtacgggg aagtaccgta tacctgtccc cacacgctat gctcattgca gggtaggaga
     3001 atataagcga agcagtttat agcttatttt ttttgtaaaa tattttacca gctcgccgaa
     3061 tatgatccag tatatccgga tcgttgagat aattgtatag aaataaatct tccgtgtagg
     3121 ggagaagaag atcatcaata ccagtcatga ttttacctaa agtggtaagg tttaaatcac
     3181 tactgccaat taatgttaaa tcaatgtcag atccattttt gtaatttcct tttgctctgg
     3241 aaccataaat aattgctttt tcaatttctg aatatttaga aaatacagta tataatttgt
     3301 ttattgtatc aatactaagt ccaaatttat caggcattat ttttgtccaa taattttttt
     3361 aaaaaatcat gaagttttct aaattgtgga tagtaataat acattattga tgaagaaatc
     3421 ttatttgcta ttttctcatc gtaagtatgg ctggtaagaa tacgactttt aatcatatcc
     3481 atccatatct ctccatcatc aataatacct cgtttgtaag ccatacgaaa tgcatcgcga
     3541 ctacctgtta aacctgtttc gccctgcgat tcaaaataat cctttattac attccatgcc
     3601 aattcataag tatattcaaa tactttaatt aatccctgtt cttctaactc atttaattga
     3661 cctttatcaa taaattttgt caattgatca agagcctttg aaaaattgct taatcgttgt
     3721 ttccagcaaa tatcctgttg atccattgta tagtacctgt ataattgtag taagagtata
     3781 aaatactatt gtaccgtttt atcaatgaaa tttgataaac atccattaaa cactgaaaac
     3841 aataaaattt tttttattga ctatatgcca tatatttcat aagtattaaa aaaattattt
     3901 gtattcatgc tttctccagt agttagtttt caggttttaa aaataatagc ttcatactag
     3961 tgtgttttta taagtgatat atatactaca taggaagata tagttattta aagtgtacat
     4021 tacatggaac aaacaacaat aattattgcc ggtttgggca gtgcggggtt ttctgcactt
     4081 acaacggtaa agcggattaa tccaaaagca cgagttgtgg tcattgatcc aaaagaaaag
     4141 gatattgtgc atccatgcgg gttgccctat gcactggaag ggattgttag ctatgaacat
     4201 ctgagtcagg atatctatct caaccgcatg aatgttgaaa agataacggg aacggttcaa
     4261 ttggttgatg ctgcaaatca taaacttatt gttacatcag gcaacaatga acaattggta
     4321 aactttgaca gagcaattat ttgtactggt tataagcctt tggtgccacc cattgttaat
     4381 atcaaaaaat attataacaa tggtgtgtat acgctctctt ccgttcagga tttgcttgct
     4441 atcaaaagtg ctttacacgg taaacaaagg gcgctggtta ttggagcagg ggctattggt
     4501 cttgaaatgg catatgctct acaggttaac ggattgcagg taactgttat tgaagcaaag
     4561 gatgtgttgc ccaatgcgct ggacagtgat atggcatcaa tagtggcgga ctactgcaca
     4621 caaaacaata ttgctcttgc attacatgct gctgtcaata ccattgaaaa aagtaataat
     4681 ttcattgcta ttaccaatga tgcacaatac aatgcagata tcattattgt tgcctgtggt
     4741 tttgcaccca actgtgattg ggctattgcc tcttcactga aaacatcaaa aggtggggcg
     4801 atagttactg ataatctaca ggtttgccct gatgtatatg ctgcaggtga ttgtattgaa
     4861 tgctggtcag ttattgataa aaaacctttg ccggtaaaac ttgcaacatc ggcatacaag
     4921 cagggtgtta ttgctgccga aaatggtatg ggtggcaatt cagtttatgt aggtactgcg
     4981 ggtacgtttg ttaccgtgat aggcaattta gagattgctg caaccggttt ttcaacagat
     5041 gtagcaaaag aacgtggttt ttccgtgata gcagggaaga taagtgctac agtaaaaccg
     5101 gaatattttc cgtccaatga acgattgact atgaaggtgc tctgtgatat acatggaaag
     5161 gtgattggag cgcaggcaat aggccatgga gcagcttcaa ggattaatat agtcagttta
     5221 gccatcaaca agggattaac cattgatgat ttattacagt ccgagatggc atactgtccg
     5281 gcagtaagtg aagttaacga tattttattg aaagcatgtg aatttgcaaa acggagaatg
     5341 aaatgaccgc acaaagaaca tttttatatg aggatcacat aaaattaggt gccaggatgg
     5401 ttgagtttgc aggctgggaa ctgcctgtca tgtatacttc aattattgat gagcacatgg
     5461 ctgtgcgtca atccgcaggc atttttgatg tgtcacatat gggcgagatt gttgcaaaag
     5521 ggcgtggtgc aaaagctgca ttacagaggc ttataccaac cgatatgggc aggcttgaga
     5581 aaggcaaatc aatgtatacc tgctttttaa atgagcaggg tggcattatt gatgatcttt
     5641 ttatcttcat gttatccgac aatgaatatt atcttgttgt taatgcaggc acaacagaaa
     5701 aggatgtaga atggttaacc ctgaattgta aaactaatgt tgcaattcag gatgtttcag
     5761 gggttacctc aaagattgat cttcagggcc cgcaatcata caatatctta aaaaagataa
     5821 ttaaggatga caggctggaa ggtttacagc ggttttactt ttttacggga acatataaag
     5881 gcgaacctgt tctggtgtca cagacagggt ataccggtga gtttggctat gagctttttt
     5941 gtatcaacaa tataatttca cgattgtgga atgatatcct tgatgccgga aaagaatatg
     6001 gcttaaaacc agcagggctt ggtgcacggg atacattgcg gttggagtca tgctactcat
     6061 tatatggaca tgaactgagt gaaacgattt caccaattga aggtggcatt ggttttgttg
     6121 ttaacgcaac atcagattac atagcaaagg atattattga agaccagaaa aaaaatggtg
     6181 ctccgcgtga aatactatgt cttgaaatga tggataaaac tatcccccgc agcgattacc
     6241 cggtagtata taataaacag gatataggcg ctataaccag tggtggattt tcacccttat
     6301 tgaaaaaggg aattgcaatg gcgctggtta agcgtggttt ggttaccata catgatgagg
     6361 tgtcagtagg tatcaggggt aaacaaagcc ctgcaaaggt tgttgcacga ccattttata
     6421 aatataatgg aaaaaggctt taatatctgt ttacaataag atgctgagat aaaccgtgta
     6481 tgaaaatata aatttattgt aggagcacta taatgagcaa tatacctgaa aaccttaaat
     6541 acacaaaaac acatgagtgg attgacgtgc aggatacgta tgccggtgta tgtggtatta
     6601 ctgatcacgc gcaggagatg ctcacggata ttgtatttgt agagttgcct gaggttggga
     6661 tagaagtcaa agctggtgag caggtggctg ttgttgaatc agtaaaagca gtgagtgatg
     6721 tgtatgcacc gataagcgga agaataaccg aagtaaacaa actgttagag gataagccgg
     6781 aacttttaaa cagtgatccc tatggtgaag gctggattta taaaatagat ataaaggata
     6841 agtatgaact ggatgaactg atggacgctg ctgcttatgc agagcatgta gcccagggtg
     6901 aatagccatg agttttttac ccaacagtga cgatgatatc cttgaaatgt gtagtgaaat
     6961 tggtgtttca agcatagaag atcttttttg tgacattcct gcttcaataa aaaatcccac
     7021 tatacaactt cctcaagcgt tatccgagca ggaagcgctt acgcatatac ttcatatagg
     7081 caatgctaat gaacaactat gttctcttgt tggtgctggt atctataatc attatgttcc
     7141 cgctgttgtt gatcatttag tttcgcgctc tgagttttat actgcctata ctccctatca
     7201 gcccgaggta agtcagggaa cgttaactgc aatctttgaa tttcaaacat ttatatgcag
     7261 gctcaccggt atggatattg ccaatgcaag catgtatgat ggtgcaacag cgttagccga
     7321 agcagtgctt atggctatac gttcaaccgg caaaaacagg gtgctgtgct cggcaacggt
     7381 tcatccccat taccgtgatg ttctttctgc ctacctgtgg gcatcggatg cgcaattggc
     7441 gatagttccc tataaacagg gcttaactga ttatgaacaa ctatcgcaca tgatagatga
     7501 caatgttgcc tgcgttattg tgcagagccc taattatttt ggagctattg aggatgttgc
     7561 aagtattgcg gatagtgcac ataaccatgg tgcggtatgt atagcaacat ttactgaagc
     7621 gttgagtttg gcattattaa aaagcccggg cagtcttggt gcagacattg cctgcggcga
     7681 ggggcaatcg tttggaaatt atcctgcatg tggaggcccg cttttaggaa ttttggcctg
     7741 ccgcagcaaa tttatgcgca ccataccagg caggctggtt gggaaaacag tggatgaaga
     7801 aggcagggat gcttttgtta tgacattgca aacacgtgaa cagcatatca gacgtcagcg
     7861 tgcaacatca aatatatgca ccaatgaagg gctatgcgct ctgcgtgcta ctatatattt
     7921 aagccttgtt ggaaataaat tgattgagct tgccaggatt aatcatgctt tagccagtta
     7981 ctgttgcagc aggcttgttg cattaggata tgagcgtgtg tttactgtgc catttttcaa
     8041 tgaatttgtt atacgggtta acaatgcaaa agaaattaga aacaggttgc ttgataacgg
     8101 gtacgcactg ggaatatcca ttgataccta ttatcccgag cttaacgatt gccttttgct
     8161 tggtgttacc gaaagtgtat caaacaatga tattgacaat ctggttgaat tgttgcaaac
     8221 aatttgatga gggtaactat gcagacaata ttttcatttc ataaaaaagg taaaaataaa
     8281 tatagtattc cagcctgtga tcccatacag gttgatgaaa tccccgaatc aatgctacgc
     8341 gatgatttaa cattggttga tgtaagcgag gttgatgtgg tgcgtcatta tcggaaactt
     8401 tcatctttaa actttggtgt tgataatggt atgtatccgc tgggaagctg taccatgaag
     8461 tataatccca agataaatga tgttgtagcc atgctgccgc agtttgcgat gcagcatccg
     8521 gctaccattg aggaaacagg gcagggcgc
//
LOCUS       DAOSOX010000095         8547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8218_length_8547_cov_8.119053, whole genome shotgun sequence.
ACCESSION   DAOSOX010000095 DAOSOX010000000
VERSION     DAOSOX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_8547_cov_8.119053"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /gene="bamA"
                     /locus_tag="PLX22_07795"
     CDS             <1..1970
                     /gene="bamA"
                     /locus_tag="PLX22_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOT19842.1"
                     /translation="VQKISILGNTIFSLRTLKGLLVTEEEGLFTDAKFTKVTYDEDKG
                     KILGYYRESGYLDAEIVEDTVDYKWLDPETQEKRGIYITIKLREGERYYFDGYSVDGN
                     EVFTKEDLMSQFEQIKIGQPFNDTLFQKDRQMISFIYATKGYIFARIIPERKITEKVV
                     EVDGKKEKRKYIFTHFQIKEGPQAYIENIIIKGNIKTKDKVIRRELIVYEGELFNSYK
                     MQLSRERVYNLGFFKEVNFDVRPGSKEGYMNLIVDVVEQPSGTISLGGGYGSTTGFSI
                     FADIAENNLLGNGQRISLRFEYGPLRNSITLGFNEPWLFNKPIGFNVSIFYILSTIEQ
                     SSIFTNSNQTAKHQTQQYGYSLGLSYRFWYYYGIGSVWHHAFKKVVNATGNSTDSVFL
                     EQAQGIQEKRKLTLYAYRDSKDNYLNPTKGWRAEFSASFTGGYLLRGDDHWVMYSPDL
                     YWYFSPFHLPFLKTHPCVVELRFNGTFITPPWYKSKMYEVQDPDKNPWLESEDRLYIG
                     GPETLRGWEVLDLDFPDSWRYGLFHRILYGAEFRVPIHPTFLWLVFFFDAGSVWTDSF
                     WEKSLEESTREIINEDKVNKLVYDIRDITDVDVMSYFKYSYGFGFKIQLPMMPLRFWF
                     GRKLAWVGKDEGYFKPLSGFQFQFGIGDMRF"
     assembly_gap    2081..2180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2265..2936
                     /locus_tag="PLX22_07800"
     CDS             2265..2936
                     /locus_tag="PLX22_07800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOT19843.1"
                     /translation="MPLIKLVVGTTMVVMLACSGDVYYKNSGQKLLKTYKGNYAIVVS
                     KKNFALFVVSREKIIKKYTIAYGLNPDKKPKLYENDNRTPEGLYYVTEILSMDAPKHS
                     SSYKKLQGMNRVYFKSSKGHYKFGYPDVDLGDNAYGPRFYRLSYPNDNDIKRYEQAKI
                     HGALPLYNGKMPSIGGGIAIHGNNDPDSIGHLATSGCIRMLNDDVIELEQFIQIGTPV
                     YIVPD"
     gene            3140..3466
                     /gene="yajC"
                     /locus_tag="PLX22_07805"
     CDS             3140..3466
                     /gene="yajC"
                     /locus_tag="PLX22_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT19844.1"
                     /translation="MRGIKVFPFEEIAYASNGQQSSSPWVSIIPLVLMIVIFYLLLIR
                     PQQKKEKDRMKMINQLKKGDKVLTSSGMIGVIVNIKPEENIITLKLAEGVKVDFVKSA
                     IQQKLS"
     gene            3853..4485
                     /locus_tag="PLX22_07810"
     CDS             3853..4485
                     /locus_tag="PLX22_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19845.1"
                     /translation="MKTYTNKLPLKLMCGVCLLFSTLMLYGPAIAQDQNPQTVKQPVA
                     EDTVVQNKTNQQELSPQQNVVEDTTTAEQAPAHEVDTTEKKVVIKEEPKKEAAKPQTK
                     AREVIDQKIQGNDTVTVGTNSLLSITDGTFRYSRIPGITIKENNEPILTVVKEEVPET
                     VEEKGIFGLSKKASDRIAKIILVVIVIFVFILYRMRTKNTRGSVLRRFPK"
     gene            4508..6163
                     /gene="secD"
                     /locus_tag="PLX22_07815"
     CDS             4508..6163
                     /gene="secD"
                     /locus_tag="PLX22_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT19846.1"
                     /translation="MSRGLIYKGIFIVCIILFAIILILPTVGKNTLRVVMSTTATQED
                     IQAVKLRFPESNYTITARENEFFIEGYNLNVAVMNEVKQYHGVVDAQLLPHWAEKALL
                     AKKINLGLDLQGGSYLVLQANYHKIEEKTGKPLTNTEKNEITEQALELLRNRVDKFGV
                     AEPSIRPKGNDAIEIQLPGVKDPEAVKKAIGTTGQVEYRLVDDVYTQKANQWLKQYYA
                     GKALPEEQLELNKLADTLSNAIALPDTLELMFYYERHPQTGKLFAAYPMVLEKKVALA
                     GSDISKAWVATDEYQQLAVHFNTTPEGAAKFAEVTSEKNQGRKLAIVIDDKVRSAPSI
                     RVQINTGQAIIQGDFTHEEVTALARIIKEGALPVDLSIVEERTVGPTLGLDSIQSGVK
                     AFVVGVTGIMLFMFVYYKFSGLIADFGLLLNGIFMLALLSWLGFTLTLPGIAGFILTM
                     GMAVDANVLIYERIKEELRAGKTPRMAVTQGFSRAFWTIFDSNITTLLSAFVLSQFGT
                     GPIKGFAVTLSIGIICSMFVALYITRFIFEMITSRGTLQKLSI"
     gene            6176..7066
                     /gene="secF"
                     /locus_tag="PLX22_07820"
     CDS             6176..7066
                     /gene="secF"
                     /locus_tag="PLX22_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT19847.1"
                     /translation="MEKIIPFFKYRFVAYSLTVVLIIIFSLQTYRNGGFNWGIDFVGG
                     AKITVKFQQGVDTAQIRKVLAHNAINATIQQVGKDEDNEYIIATKLTSRTENVEESTK
                     ALRTILQTNFNNAEVLSIETVGPAIGDFLKRSAGKLIALSIIIMTVYLAFRFELKYSV
                     GAMVALIHDIILTVLFCGFANIEINIPILAAILTIYGYSVNDTIIIFDRVRENLQITT
                     KQAFIDVIDKSITQTISRTLLTLLTTLFAVFALYMMGGEGLNEFATILLFGLSIGTFS
                     SLYIASPFVMYWEKLTSKQS"
     gene            7069..7947
                     /locus_tag="PLX22_07825"
     CDS             7069..7947
                     /locus_tag="PLX22_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19848.1"
                     /translation="MPLWHAFIFFCSILVASCAIYAQVPDAYSYNSIAAFANWLVEQK
                     EYYRAYQEYRRLHAYYPDRYAYDSFVVASLYCQYEGKQYAEILSSANFTTTAPPWMWI
                     YIFDSAFKCNDSSVNHGIATIPSYEYSSDINEIIFKRKLAYCLYAGHDYQELSPTQSF
                     VDSYDVESLYAYSRFKRSQLKNPYAALAWGMVPGGGYVYSGNTEDGIVAAIVIGICSV
                     ITYFAIDTNNTGIAVFTGSIGVFFYGGSIAGGYLASIKANKAIMSELQAYLEEGLKFH
                     DDRAMMFERYGKPKTQ"
     gene            7925..>8547
                     /gene="ribD"
                     /locus_tag="PLX22_07830"
     CDS             7925..>8547
                     /gene="ribD"
                     /locus_tag="PLX22_07830"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOT19849.1"
                     /translation="MENQKHNDYMRMALRLAFGRLGATSPNPAVGAVIVKNGQVVSTG
                     CTMEYGGDHAEVVAIKNASCNLQGAEMYVTLEPCCHQGKTPPCTNAIIASGIATVYIP
                     MLDPNPLVAGRGMQTLQQAGVEVVVLHQCKQAAFDIVRPFIKSIARHKPFVIHKIAST
                     LDGRTATNTGDSRWISSEVSRLVVHRLRSCVDTIVVGSSTVEADNPRL"
BASE COUNT         2613 a         1425 c         1894 g         2515 t
ORIGIN      
        1 aggttcagaa gatttcaata ttagggaata ctatcttttc gttgcgtaca ttaaaaggac
       61 ttttagtaac cgaggaagaa gggctattta cagatgcaaa atttaccaag gttacctatg
      121 atgaggataa aggtaaaata cttgggtact atcgtgaatc aggatatctg gatgccgaga
      181 tagtagagga taccgttgat tacaagtggc ttgaccctga aacgcaagaa aagaggggta
      241 tctatatcac catcaagtta cgtgaggggg aacgatatta ctttgacgga tattctgttg
      301 atgggaatga agtttttact aaagaagatt taatgtcaca atttgagcaa atcaagattg
      361 gacagccttt caatgataca ttgtttcaaa aagataggca gatgataagc tttatttatg
      421 ctactaaagg atatatattt gcccgcatta tccctgaacg aaaaataact gaaaaggtag
      481 tggaagttga tggtaaaaaa gaaaaaagaa aatacatctt tacgcatttt cagattaagg
      541 aaggaccgca agcctatatt gaaaacatca ttattaaggg caatattaaa accaaagata
      601 aggttatacg acgtgagtta atagtgtatg aaggtgagct tttcaattcg tacaagatgc
      661 agttatcccg tgaacgtgtt tataatttag gattttttaa ggaggtcaat tttgatgtaa
      721 ggcctggttc aaaagaagga tatatgaatc ttatcgttga tgtagttgag cagccttccg
      781 ggaccatttc acttggtggt ggttatggtt ctacaacagg tttttccatc tttgcagata
      841 ttgctgaaaa taacttgctg ggtaatggac agcgtatatc cttgcgtttt gagtatggcc
      901 ctttgcgtaa ctctattacg ttaggtttca atgagccatg gctttttaat aaaccaatag
      961 gtttcaatgt ttcaattttt tatattttat caactatcga gcagagctca atttttacca
     1021 attcaaatca aactgcaaag catcaaacgc agcagtatgg ttactcactg ggattaagct
     1081 atcgtttctg gtactattat gggattggtt cagtatggca ccatgccttt aaaaaggttg
     1141 tcaatgctac gggtaacagt accgatagtg tatttttgga acaggcgcag ggaattcagg
     1201 aaaaacgaaa gctgacactc tatgcgtatc gtgactcaaa agataattat ttaaatccta
     1261 caaaagggtg gcgtgcagaa ttttcagcat catttaccgg cggctatctg ctacggggcg
     1321 atgaccactg ggtgatgtac tctcccgatt tgtactggta cttttcgcct ttccatttac
     1381 cgttccttaa aacacatccc tgtgttgttg aattgcgatt taatggaact tttataactc
     1441 cgccatggta taaaagtaaa atgtatgagg tacaggatcc tgataaaaat ccatggcttg
     1501 aatcagaaga caggctttat attggtggtc ctgaaacatt gcgaggctgg gaggtgctgg
     1561 atcttgattt tcctgattca tggcggtatg gattgtttca caggatactt tacggagctg
     1621 aatttagagt tcctatccat cccacatttt tgtggctggt atttttcttt gatgctggtt
     1681 cggtatggac agacagtttc tgggaaaaga gcctggagga atctaccagg gaaattatca
     1741 atgaagacaa agtaaataaa ctggtgtatg acatacggga tattactgat gtagatgtca
     1801 tgtcatattt taaatactca tatggatttg gctttaaaat acagttgcca atgatgccgt
     1861 tgcgattctg gtttggacga aaacttgcgt gggttgggaa ggatgaaggt tattttaaac
     1921 ctttaagcgg cttccaattt cagtttggta taggagatat gcgtttttga gtatgcggtt
     1981 taattggatt tatacactat taattttcat ggtattgtta ttatgttgtt cttcattaaa
     2041 aaaagagcag gatacaattg ctattcgcta tgttcagttg nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn tgggtatgat ataaccaatg aggtattaaa agagcttgtg
     2221 gcaacacgac tgcaaaaatc accggttaca cgatagaggt aattatgcca ttaataaagt
     2281 tagttgtagg tactacaatg gtagtgatgc ttgcgtgcag cggagacgtg tattataaaa
     2341 attcagggca gaagcttttg aaaacgtata agggcaacta tgcgatagtt gtcagtaaga
     2401 aaaattttgc tttatttgta gtaagccgtg aaaagattat aaaaaaatat accatagcgt
     2461 atgggcttaa tcctgataaa aagccaaagc tttatgaaaa tgataataga actcctgaag
     2521 ggttgtatta tgtaactgag atcttgagta tggatgcacc aaaacattcc tcgtcgtata
     2581 aaaaattaca ggggatgaac agggtttatt ttaaatcaag caaaggacat tataagtttg
     2641 ggtatcctga tgttgattta ggtgataatg cgtatggccc acggttttat cgtctttcat
     2701 atcccaatga taatgatatc aaacgatatg aacaggcaaa gatacatggt gcattgccgc
     2761 tgtataatgg caagatgcct tcaattgggg gaggaatagc tattcatggc aataatgatc
     2821 ctgattcaat agggcattta gcaacaagtg gatgtattcg gatgttaaat gatgatgtca
     2881 tagagcttga gcagtttatc cagattggta cacccgttta tatagtacct gattaatatt
     2941 aatttgggta aattgtgttg acatttggtg atagttctta aaaaggatat acaggttaaa
     3001 tactgatttt tattatgtat agcaatcaag caatcagtgg ttgtttaaca gcgtatgtaa
     3061 aatgtttcaa acaataaaac tgattgacat ttgctgttta taccataatg gttgcaatgt
     3121 tttatagtaa agattaacaa tgagaggtat aaaagtgttc ccatttgaag agatagcata
     3181 tgcaagcaat ggacagcaat catcaagccc atgggtaagt atcattcctt tagtactcat
     3241 gatagtgata ttttatcttc ttttgatacg gccgcaacaa aagaaggaaa aagatcgtat
     3301 gaagatgata aatcagctta aaaaaggtga taaggtcctt acctcaagcg gtatgattgg
     3361 tgttattgta aatattaaac ctgaagaaaa tattataacc cttaaattgg ctgaaggggt
     3421 aaaggtagat tttgtaaaaa gtgccataca acaaaagctt tcttaaagga tagcattaat
     3481 attatttttt aatattattt tgtcatctta atacatggac agtggttgcc ggccaaccgt
     3541 agatgatgaa gataattggc gacagtcata ctgaatcatg atgcaggata tagtgttata
     3601 tattccgggc caggcacggg gttgacgata tagtggtata tcatttctga acggagtgaa
     3661 gaatcctgct gttgcaaaga gagggaaaca atgctcccct cgccttgtga agaagagtag
     3721 ttggggtaag gtgcataatc atttttttag gcgagaaacg attcctgata tccttgcaca
     3781 tatgctgttg ctgagttgta tagaagtgat ggtcaatagt tgagtctgat aatcgtattt
     3841 tttggagaag atatgaaaac atatactaac aaactaccat taaagttgat gtgcggtgtg
     3901 tgtttactct tttcaactct tatgctgtac ggaccggcaa ttgctcagga tcagaatcct
     3961 caaactgtta agcaaccggt agctgaagac actgtagtgc aaaacaaaac caatcaacag
     4021 gaactatcgc cacagcaaaa tgtagttgaa gatacaacaa cagcagaaca agcaccagca
     4081 cacgaagttg atacaactga aaaaaaagtt gttataaaag aggagccaaa aaaagaggct
     4141 gccaaacccc agacaaaagc cagagaggtg attgaccaga aaatacaggg taacgatacg
     4201 gtaaccgtag gtaccaattc tttattgagt atcaccgacg gaacatttag atattcgcgt
     4261 attccgggca ttactataaa agaaaataat gaaccgatcc taaccgttgt aaaagaggag
     4321 gtgcccgaaa cagttgaaga aaagggtata tttggattaa gtaaaaaagc atctgacagg
     4381 attgctaaga ttattcttgt tgttattgtc atatttgtat ttattcttta tcgtatgcga
     4441 accaaaaata cacgcggaag tgtattgcgc agatttccga aataacatca cattacatga
     4501 ggtatacatg tcacggggat taatttataa gggtattttc atagtttgta taatattatt
     4561 tgcaataatc cttattttac caacagttgg aaaaaacaca ctacgggttg ttatgagtac
     4621 aaccgcaaca caggaagata ttcaggcggt taagttacgc ttccctgaat caaactatac
     4681 cattactgcc agagaaaatg aatttttcat tgagggatac aacctcaacg ttgcagtaat
     4741 gaatgaggtg aagcagtatc acggtgtagt tgatgcgcaa ttgctgcctc actgggctga
     4801 gaaagcattg cttgcaaaga agataaattt aggattggat ctgcagggcg gatcatattt
     4861 ggtattacag gcaaattacc ataaaataga agagaaaaca ggaaaaccat taaccaatac
     4921 tgaaaaaaat gagataactg aacaggcgct ggagctgctt agaaaccgtg ttgacaaatt
     4981 tggtgtggct gagccatcca tacgtcccaa agggaatgat gccatagaaa tacagcttcc
     5041 gggtgtgaaa gatcctgaag cggtaaaaaa agctattgga accacagggc aggttgaata
     5101 ccgcctggtt gatgatgtct acacacagaa ggctaaccag tggttaaaac aatattatgc
     5161 cggtaaggct ttaccggagg agcagcttga actgaataaa ttggctgata cactttcgaa
     5221 tgccatagcc cttcctgaca ctctggagct tatgttttat tatgaacgcc atccccaaac
     5281 tggcaagctc tttgcggcgt atcctatggt tctggaaaaa aaagttgccc tggctggcag
     5341 tgatataagc aaagcgtggg tggctaccga tgagtatcaa caattagcag tacatttcaa
     5401 cacaacgccg gaaggtgcag caaagtttgc cgaggttacc agcgaaaaga atcaggggcg
     5461 taagctggcg atagttattg atgataaggt ccgcagcgcg ccttctatac gtgtacagat
     5521 aaatacagga caggcaatta tacagggtga ctttacccat gaagaggtaa ctgcgcttgc
     5581 acgaataatt aaagaaggcg cattgccggt agatttgtcc atagtagaag agcgaacggt
     5641 tggtcctacg ctgggacttg attcaatcca gagcggtgta aaggcatttg ttgttggtgt
     5701 taccggcatt atgcttttta tgtttgtgta ttataaattt tcagggctca tagccgattt
     5761 tggtttgctg ctcaatggta ttttcatgct ggcgcttttg tcatggcttg gttttacatt
     5821 aacactgcca ggtattgctg gttttatatt gacaatgggt atggctgttg atgccaatgt
     5881 tctcatctat gagcgtataa aagaagaact gagagccggg aaaacaccaa ggatggcggt
     5941 aacacagggc ttttcacgag cattctggac tatttttgac tctaatatta ccacactgct
     6001 ttcagcattt gtactttcac agtttggtac gggccccatc aaggggtttg ctgttacgct
     6061 ttctataggt attatatgct caatgtttgt agcattatat attaccagat ttatctttga
     6121 aatgataact tcacgcggaa cattacaaaa actaagtatc taaggggtgc tgacattgga
     6181 aaagatcatt ccatttttta agtatagatt tgttgcgtat tcacttacag tagtgttgat
     6241 aattattttt tcattacaaa catatcgcaa tggtggcttt aactggggta ttgattttgt
     6301 aggtggtgct aaaattacgg taaaatttca acaaggtgta gataccgcgc aaatacgaaa
     6361 ggtgctagca cacaatgcga taaacgctac catacagcag gtgggcaaag atgaagataa
     6421 cgaatatatt attgcaacaa agctaacaag cagaaccgaa aatgtggaag agagcaccaa
     6481 agccttacgc acgatactac agaccaattt taataatgct gaggtgctga gcattgaaac
     6541 agtgggtccc gccataggtg attttttaaa aaggtcagca gggaagctca ttgctctttc
     6601 aattattatt atgaccgtgt atttagcatt caggtttgag cttaaatatt ccgttggtgc
     6661 aatggtggcg ctgattcatg atattatcct tacagttctt ttttgtggct ttgcaaatat
     6721 cgaaataaac atccctatcc ttgcagcaat ccttactatt tatggctatt cggttaatga
     6781 caccatcatt atttttgacc gtgttcgtga aaatttacag attacaacaa agcaggcttt
     6841 tattgatgtt attgataaat ccataacaca aacaatatcc agaacattgc ttaccctgct
     6901 gacaacgctt tttgcagtgt ttgccttata catgatgggt ggtgaaggct taaatgaatt
     6961 tgcaacgata ttgctctttg gattgtctat tggaacattt tcatcattat acattgcatc
     7021 accatttgtt atgtactggg aaaaactaac atcaaagcaa tcgtaattat gccgctatgg
     7081 catgccttta tctttttttg cagtatactg gtagcttcgt gtgctattta tgcacaggta
     7141 ccggatgctt atagctataa cagtatagct gcctttgcta actggcttgt agagcaaaag
     7201 gagtactacc gtgcgtatca ggagtatagg cgtttacatg catattatcc cgataggtat
     7261 gcgtatgata gctttgttgt tgcttcgttg tactgccagt atgaaggaaa gcagtatgct
     7321 gaaatactat cctctgcaaa ctttaccact acagcacctc catggatgtg gatctatatc
     7381 tttgacagcg cattcaaatg caatgattca tcggttaacc atggtatagc aactatccca
     7441 tcgtatgaat acagtagcga tatcaatgaa ataattttta aaagaaagct tgcctattgt
     7501 ttgtatgcag ggcatgatta tcaggaacta tcgcccacac aatcatttgt tgacagctat
     7561 gatgtagaaa gcctgtatgc ctatagccgt tttaaacgct cacagctaaa aaatccctat
     7621 gcggcacttg cgtggggcat ggtgccgggc ggcggctatg tgtacagcgg caataccgag
     7681 gatggtatag ttgcggcgat agttatcggt atatgcagtg ttattaccta ttttgccatt
     7741 gacactaaca atactggcat tgctgtattt accggcagca ttggcgtatt tttttatgga
     7801 gggagcattg caggcgggta tcttgcaagt ataaaggcaa ataaagcgat catgagcgaa
     7861 ttgcaggcat atttagaaga aggcttgaaa tttcacgacg atagagcaat gatgtttgaa
     7921 cggtatggaa aaccaaaaac acaatgatta tatgagaatg gcattacgcc ttgcatttgg
     7981 cagattggga gcaacctcgc caaatcctgc tgttggtgcg gtcattgtaa aaaatggtca
     8041 ggtggtgtcg actggttgta ccatggaata tggcggtgac catgcagagg ttgtagctat
     8101 aaaaaatgct tcatgcaacc tgcagggagc ggagatgtat gtaaccctgg aaccatgttg
     8161 ccatcaggga aaaacaccgc cctgtaccaa tgccattatt gcatcaggta ttgctacagt
     8221 atatattccc atgctcgatc ccaatccact tgttgcgggg agaggaatgc aaacattgca
     8281 acaggcaggt gtggaggttg ttgttttaca tcaatgcaag caggctgcct ttgatatagt
     8341 gcgtcctttt atcaaaagta ttgcgcggca caagccattt gttatccata aaattgcatc
     8401 aacgcttgat ggcagaactg caaccaatac aggtgattcg cgatggataa gctcggaggt
     8461 ttcacgcctt gttgtacaca ggctgcggtc atgtgttgat accattgttg ttggtagctc
     8521 caccgttgag gctgacaatc cgcgcct
//
LOCUS       DAOSOX010000096         8492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8294_length_8492_cov_8.161432, whole genome shotgun sequence.
ACCESSION   DAOSOX010000096 DAOSOX010000000
VERSION     DAOSOX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8294_length_8492_cov_8.161432"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1215
                     /locus_tag="PLX22_07835"
     CDS             394..1215
                     /locus_tag="PLX22_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT19850.1"
                     /translation="MSKRPAWWLVILAKIWPITWLSARATQSKIIGPIVKRTMLPFFN
                     KKNLNITYLPINITVAPSSTPIPLLIVEAIIKRSAHRVIIKRCTCRDAKKCREHPIDY
                     GCLLMGEGAKEIDVRVADHVTINEAIAHLHKCIEEGLIPMVGRVKIDNLIWGVKDTGK
                     LLTMCFCCRCCCTILNSGKYLPAGAAASIVRLQGITITVDRNACIGCGTCIDECFMDA
                     IRIIDGTAVHNDAICKGCGRCAYVCPHNATSLSISNVDAAVTEIYSRIAQYMQYE"
     gene            1224..1490
                     /locus_tag="PLX22_07840"
     CDS             1224..1490
                     /locus_tag="PLX22_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19851.1"
                     /translation="MISNKYFWYVLMSGALVLWAFAIALIYLFPEWDYKAVLFIGLII
                     LHGVEILYVFTILKKDVSTVTIAIKTFLFGFTWWLPVKKGILQG"
     gene            1495..2322
                     /locus_tag="PLX22_07845"
     CDS             1495..2322
                     /locus_tag="PLX22_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT19852.1"
                     /translation="MRLQYGPNKTIVRLVVMLSWPIILLGKRLSSYPVLKHIINPFFK
                     YPHNEVTAVPINVAIAPPDYVSLPVEVLVEVVNRVHAIFILDECICRGLMHCKNHPVS
                     IGCMALGEASLTIHPSHGHRATNEEAIAHINKAAQAGLVANVAHVWIDPVAFFSVPFH
                     KLMFICFCDDCCCLYRKHMKKRGENLNKAYKKLPGVSVIINHDICNGCGVCAEQCFVA
                     AIDMKDGSPVIGSDCKGCGRCVQSCPVHAVHMQFEDKNTLVKNLVERINAVADIGLQ"
     gene            2465..2848
                     /locus_tag="PLX22_07850"
     CDS             2465..2848
                     /locus_tag="PLX22_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19853.1"
                     /translation="MKIEQRINYGYIIYDITGDITDENFVEIEDEIKNTLPENFSNVI
                     LNIERVPYINSSALGWLVKLMREVTLKGYQLFLMNVNHEIMGLLKLTNTLSFFKIVPD
                     EQFLIEKEKRKDLDKILGDVDDNDI"
     gene            complement(3058..4014)
                     /locus_tag="PLX22_07855"
     CDS             complement(3058..4014)
                     /locus_tag="PLX22_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOT19854.1"
                     /translation="MLYCQAQSFQAVRQAIALSLATLKECTITIAPQVFTHPHYGRMV
                     HSLINVLQQLSVAISITDTTITLTPHQLQSFTATIALHPYCPIADIFLSMAPALIHNE
                     VQSDILFHGVTHSQFSHSTGFIRYGLAPLLQQFGCYLFSATKKFGFYSATGTAATKVY
                     PALHKNAGAIVTINRISGVKIYIASINQEFALYQKQKLAQKLSLSDDTIHIVQIANVT
                     NHGNAVDVFFISDTIPGMLSFTLPLYDANGAFIFEDSMMDTFIEQVANECQKYYDYFP
                     LPIIEEALPFVVNAGHDYTIVQKMNPAVATTESYELCQLFTQ"
     gene            complement(4014..5282)
                     /gene="serS"
                     /locus_tag="PLX22_07860"
     CDS             complement(4014..5282)
                     /gene="serS"
                     /locus_tag="PLX22_07860"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT19855.1"
                     /translation="MIDPKIVRDNIDLVKHTLKIRRMEDAVDIAKLEDMDATRRQIIA
                     KIDELRTMRNTLSKEVGTLKQKGQNPTDIMEKVRSIGNDIKQYEEQIASIEETYTTMM
                     LSIPNMLHESVPIGNDEHDNVVVRTWGQKPYFDFEPKPHYDLGTQLNILDFERGVKIA
                     GARFYVYRGLAAQLERAIINFMLDLHTKKHGYTEVFGPFIVNDESMIGTGQYPKFMDE
                     YYRIERDALSLIPTAEVPLTNLYRDEIIDGNNLPLYVTMQSSCFRREAGAAGKDTRGL
                     IRVHQFQKVELVKFVEPETSFDELERLTADAEEVLQLLNLHYRVVLLCSADTSASSSK
                     TYDIEVWMPGLDRYVEISSCSNFVDYQARRARIRYRKKEGEKPMLVHTLNGSGLAAGR
                     TLAAIMENYQTRDGNITIPELLKPYLEKSI"
     gene            5888..6097
                     /locus_tag="PLX22_07865"
     CDS             5888..6097
                     /locus_tag="PLX22_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19856.1"
                     /translation="MATEKPGYGDSYSETTTRKRAMNSIMERSMDMAVENTVMAGYAV
                     TKGDGVAMVLDITFQWEMRIFVEKK"
     gene            complement(6656..6728)
                     /locus_tag="PLX22_07870"
     tRNA            complement(6656..6728)
                     /locus_tag="PLX22_07870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6693..6695),aa:Phe,seq:gaa)
     gene            6767..7693
                     /locus_tag="PLX22_07875"
     CDS             6767..7693
                     /locus_tag="PLX22_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19857.1"
                     /translation="MSIQKFLMMLFLWVIIAFLFPLPMYADEDAGYYYQKAQLQYQHQ
                     MYTYAVENLERVLIITPKHTAALNLLATIYLNHYNNRVRALQYYNQSLAIDDNQPDVH
                     CEVGKLYVYFSEYNHAIEHFTKTIAQQGKSVEAHYHLALVYNTLKQYGTAAVHADECN
                     RLTKNDTDMLLTMAADALQKNEKGVAIAQYSRALQVNPACKEAYIGLALLYRQDNSLA
                     KAIEVLEQCIKVYPHDNTTAMTLAHLCFEYKPAKRRKYYIDRAITLCKGVVERDPFHC
                     EAYSLLYDVYKTLHDVPLMDNYAEEYNRCCEK"
     gene            7911..8213
                     /locus_tag="PLX22_07880"
     CDS             7911..8213
                     /locus_tag="PLX22_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT19858.1"
                     /translation="MDSKVEYIIQKIVSIIVEKADPARIILFGSRAKGNAHKGSDIDI
                     ALEGEKALTFREMRKMKELIDDVSGLYSVDIIVLRKVDDTFKDSIKESGVVIYEKK"
     gene            8200..>8492
                     /locus_tag="PLX22_07885"
     CDS             8200..>8492
                     /locus_tag="PLX22_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HOT19859.1"
                     /translation="MKKSEIYLSVKKLENAVTSLKEAVSIAQTDLEKDGTIQRFEFTV
                     ELLWKTLKTILLYNGVECNNPRACIKEAFRNRIIEDDEILLDMLEDRNVSAHI"
BASE COUNT         2648 a         1440 c         1863 g         2541 t
ORIGIN      
        1 ccgataacta tcgcatagca taattattat gatgtcaaga agataaaatg tatgggtcat
       61 gcactaattt ttatcgcaag gtataggtgc accattgcaa ggtgagtgta gcaattcata
      121 agaagacttc cattattgtt tttgcaatgt tgtgtatcat ctattaaatg tgcaaaacta
      181 ttttatactg aaagtttgta ttttcatgtt attggcgata tcatgtagca agctaatcaa
      241 aaaatatgct tcactgctcc aattaaaaag tgcaattaaa tggtgcattt tgaaatttgg
      301 tatcaattat taatttttca aataaattat tgctggacta taattatata ctatgtttta
      361 aataatagca tcgaatattg agagataacc tgtatgtcaa aacgtccagc atggtggctc
      421 gtgattctgg caaagatttg gccaataaca tggttaagtg cacgggcaac acagagtaaa
      481 attattggac ccatcgtaaa aagaacaatg ctaccttttt ttaacaaaaa aaatctcaat
      541 atcacctatc tgccaatcaa tataactgtt gctccttcaa gtaccccaat acctttattg
      601 attgttgagg cgataatcaa aagatcagct catcgtgtta ttataaaacg atgcacatgc
      661 agggatgcaa agaagtgcag ggagcatcca atagattacg ggtgcctgct tatgggcgaa
      721 ggggcaaaag agattgatgt acgtgtagcg gatcatgtta caataaacga agcgatagct
      781 catctgcata aatgtattga ggaagggctt atccctatgg taggaagggt aaagattgat
      841 aatctgatat ggggtgtaaa agacacgggg aaacttttaa ccatgtgttt ttgctgccgc
      901 tgctgctgta ccattttaaa ttcaggtaaa tatcttcccg cgggtgcagc ggcatctatt
      961 gtccggcttc agggaataac cattactgtt gaccgtaatg catgtatcgg gtgtggcact
     1021 tgtattgatg aatgctttat ggatgctata cgtataattg atggcactgc agttcataat
     1081 gatgcaatct gcaaaggatg cgggagatgc gcttatgtat gtccacataa tgcaacatct
     1141 ctatcaatca gcaatgttga tgcggcagta acagaaattt attcccgcat tgcgcagtat
     1201 atgcagtatg aataggaggt taaatgataa gcaataaata tttctggtat gttttaatgt
     1261 caggagcatt ggttttgtgg gcatttgcta ttgcattgat atatttattt cctgagtggg
     1321 attataaggc tgtgcttttt atagggctga ttattttgca tggtgtcgaa atcctgtatg
     1381 tgtttactat attgaaaaaa gatgtatcaa cagtaactat cgccataaag acttttttat
     1441 ttggctttac ctggtggcta ccggttaaga aaggcatatt gcaagggtaa tagtatgcga
     1501 ttacaatatg gccctaataa aacaatcgtt cgtctggttg taatgctttc atggcctata
     1561 atactattgg gcaaacggct aagttcatat cctgtattaa agcatatcat caatcctttt
     1621 tttaaatatc cacacaatga agttactgca gttcccatta atgtggcgat agctcctccg
     1681 gactatgtat cattgcctgt tgaagtgctg gtggaggttg taaatcgtgt tcatgctatc
     1741 ttcattttag atgagtgtat ctgccgtggt ctgatgcatt gtaaaaatca tcctgtcagt
     1801 attggatgca tggctctggg tgaagccagt ttaaccatac atccatcaca tggacataga
     1861 gcaacaaacg aagaagctat agcacatata aacaaggcag cacaggcagg gctggtggca
     1921 aatgtggcac atgtgtggat tgatccagtt gcattttttt cagtgccttt tcataagctt
     1981 atgttcatct gtttttgtga tgactgctgc tgtctttata gaaaacatat gaaaaagcgt
     2041 ggagaaaatt taaataaagc atataaaaaa ttaccaggtg tttcggttat cattaatcat
     2101 gatatttgca atggctgcgg tgtttgtgct gaacaatgtt ttgttgcagc aatagatatg
     2161 aaagatggaa gcccggttat agggagcgat tgcaaaggtt gtggaaggtg tgtacagtcc
     2221 tgtccggtgc acgcggtaca catgcagttt gaagataaga acacacttgt aaaaaatttg
     2281 gtggaacgca ttaatgctgt agcagatatt ggattacagt agttgtaaaa acgtaaacaa
     2341 tgtatgaaat gataaacagc cgtgatacta aaaatacttt tgtaacatta attatcggtg
     2401 ttataattat tgactatcat tacatactgt gatataaatg tgtagagagg ttaggatttg
     2461 tagtatgaaa atagaacaac gtattaacta tgggtatatt atttatgata ttacagggga
     2521 tatcactgat gaaaactttg ttgaaataga agatgaaata aaaaacaccc tgcccgaaaa
     2581 ttttagtaat gttattctta atatagaacg tgtaccctat attaatagct ctgccctggg
     2641 gtggcttgta aaactaatga gagaagttac cttaaaaggg tatcagctat ttttgatgaa
     2701 tgtcaatcat gaaataatgg gcttactgaa gcttaccaat actttatcat tttttaaaat
     2761 cgtaccagac gaacagttcc ttatagaaaa agaaaaaaga aaagaccttg ataaaatatt
     2821 aggtgatgtt gatgataatg acatataaat cagcactgtt acgcttaaaa ggtcgcattt
     2881 ttaattttta aaaatatata gtaagggtta aaataaacca gcatcacctt gtcattacat
     2941 ttctggatat actgtagtcc accatcaaca atagtgttgc atatcacagc aagctattat
     3001 gataaaaagt aaattaaatt tgtaagccaa ctctatggat gaatggtgat actattttta
     3061 ttgtgtaaat aattgacaca gttcatagct ttcggttgta gcaactgcag gattcatttt
     3121 ttgtactata gtgtaatcat ggccggcgtt tactacaaag ggtagtgctt cttctataat
     3181 gggtaatgga aaatagtcat agtacttttg acattcgttt gctacctgct caataaacgt
     3241 gtccatcatg gagtcttcaa agataaaggc gccatttgca tcatacagtg gcagggtaaa
     3301 cgaaagcatg cctggtattg tatcgcttat aaaaaacaca tctactgcat taccatgatt
     3361 ggttacattg gcaatctgta caatgtgtat ggtatcatca ctgagtgata gtttttgagc
     3421 aagcttttgt ttctggtaca atgcaaattc ctggttaatg ctggcaatgt atatttttac
     3481 accgcttatt cgattaatag taactatcgc cccggcattc ttgtgtaatg cagggtatac
     3541 ctttgtggct gctgtgccgg tagcagaata aaagccaaat ttttttgtgg cgctgaacag
     3601 gtaacatcca aactgctgca acagcggtgc taagccatag cgtataaaac cggtagagtg
     3661 tgaaaactgg gagtgagtta caccgtggaa aaggatatcg gattgcacct cattgtgtat
     3721 cagggcagga gccatgctaa ggaaaatatc agctatagga caatacggat gcagggcgat
     3781 agttgctgta aaactttgca gctgatgcgg cgtgagggtt atggtggtat ccgtaattga
     3841 gatggcaacc gatagctgtt gcaatacatt gataagcgag tgcaccatcc tgccatagtg
     3901 aggatgggta aaaacctgcg gggcaatggt gatggtacat tctttcagtg ttgctaatga
     3961 cagagctatt gcctgccgca cagcctgaaa cgattgtgcc tgacaataca gcattatatg
     4021 cttttttcca gatagggttt cagcagttcg ggtattgtta tattgccatc gcgtgtttga
     4081 tagttctcca taatggctgc cagtgtgcgc cctgccgcaa gccctgaacc attgagtgta
     4141 tgtaccagca tgggcttttc gccttctttt ttgcggtagc ggatgcgcgc acgccgtgcc
     4201 tgataatcaa caaagtttga gcatgatgat atttcaacat atcgatccag tccgggcatc
     4261 catacctcta tatcatacgt tttagaagaa gacgctgatg tgtcggcact gcacaggagc
     4321 actacccggt aatgcagatt gagcagttgc aaaacctctt ctgcatcggc tgtcaacctt
     4381 tccagttcat caaatgatgt ttcgggctct acaaatttta ccagctcaac cttttgaaat
     4441 tgatgtaccc gtataagtcc tcgtgtatct ttacctgctg cgcctgcctc gcgtctaaag
     4501 catgatgatt gcatggtaac atacaggggc aaattattcc catctataat ttcatcacgg
     4561 tacagattgg ttaatggtac ttcggcagtt ggtatcagtg acagcgcatc acgttcaata
     4621 cggtaatact catccatgaa ttttggatat tgcccggttc ctatcatgct ttcatcatta
     4681 acaatgaatg gcccaaatac ctcggtgtag ccgtgctttt ttgtgtgtaa atcaagcata
     4741 aaatttataa tagcgcgttc aagctgtgcg gcaagtccgc gatatacgta aaagcgtgct
     4801 ccggcaattt ttactccacg ttcaaaatcc agtatattga gctgtgtccc cagatcatag
     4861 tgaggttttg gctcaaaatc aaaatatggt ttttgccccc aggtgcgcac aacaacattg
     4921 tcatgttcat cgttaccaat gggtacggat tcatgtaaca tattagggat ggataacatc
     4981 atggtggtat aggtttcttc tatagaggct atctgctctt catactgctt tatatcgttg
     5041 cctatactgc gaactttttc catgatatcg gttgggtttt gtcctttttg ttttaatgtg
     5101 cctacctctt ttgagagcgt attgcgcatt gttcgcagct catctatttt tgctataatt
     5161 tgacggcgtg tagcgtccat atcctccagt tttgcaatat cgacagcatc ttccatcctg
     5221 cggattttca aggtgtgctt caccagatca atattgtcgc gtactatctt tggatctatc
     5281 atggtaaaac ctcatggtgc attaaaatag tatgtttaca atgaaattgc acatgtacaa
     5341 tgtaatactg tagcacacat gcaatgctga taaaaccatt tttttacact gataatcata
     5401 taaaaaagat ggtcatcaaa tatgtctttc atgaaatagg aatgttaagt aattttgtca
     5461 agaaagtatg gtgtatgtaa gctataaaaa acaagaaaaa attgtatatc tgtaacgctt
     5521 tacagcaaaa agaaaatcat tcaggttatg gatgtacggt gttaggttct tattgttgaa
     5581 tggttgtgta gattcataaa gttttaatgc gaaataatgg atttattgga acttgtgcac
     5641 ctctaattac cgttattgaa tatgactgag gtgtgtataa aaaggcagct atataacgat
     5701 atgagctttt gtggtataat aagggagagc atcatctttt gaagatttct ccataaaaat
     5761 tttattattt tacaatttac tttttcgatt gcatctggca caatgaagtg cgaaaacaaa
     5821 accgttttaa tgagcagaaa gctggcggga tgactgtata tatgcaggag caacaacagc
     5881 atcaacgatg gcgacagaaa agccggggta tggtgatagt tatagtgaaa caacaacacg
     5941 aaagcgggca atgaatagta ttatggagag gagcatggat atggcagtag agaacaccgt
     6001 catggcaggg tatgccgtaa caaaaggcga cggtgtagcg atggtgctgg acataacgtt
     6061 tcaatgggaa atgagaattt ttgtggaaaa aaaatagcga gtgatctcct taaatttatt
     6121 ttaaagctta tacaatagag gcgaggtaat agcagagtac agtatatgga acaggacatt
     6181 cattgagtgc atcaacggcc tgaatggagc aattaaataa tgttccgttc tttcttttgt
     6241 cacaaaagcg caacaaaaga aagtaccaat gaaaagttgt cagggcgggc gaggaagccc
     6301 ggtgtgcacc cgccgtggaa cggatatatg tgatgcaatg aaggcacacc gccgtttaag
     6361 gtggctccac agggttagta agggaatatt gagcgcataa atgctcactt acgaaaggga
     6421 aaacgaagat cgctttatgc agggcacaat agagaatgcc atactggcag gagcagacat
     6481 gatgaaggga aaatccatgc agaatgcgct ggtatataaa aactgggaga agacgcagta
     6541 cagggcagcg gactgtttaa gcgactgggc agcacaaaaa gggcgccacg atgcagaaga
     6601 ctggaagacc aatcagcaga aattgtgtga caggcacaat agagaaaata aaaattggtc
     6661 agggacggaa tcgaaccgcc gacacgggga ttttcagtcc cctgctctac cgactgagct
     6721 acctgaccag ccattatgga tggatacata aagaaggtgt tataaaatgt caatacaaaa
     6781 atttttgatg atgctctttt tgtgggtaat catagccttt ctattcccat tgcctatgta
     6841 tgccgatgaa gatgctggtt attattatca aaaagcgcag ttgcaatacc agcaccagat
     6901 gtacacatat gctgttgaaa atctggaaag ggtactcatc attaccccaa agcatactgc
     6961 agcattaaat cttttagcaa ctatctacct gaatcattac aacaataggg tcagagcatt
     7021 gcagtattat aaccaatcgc ttgcaattga tgataatcag cctgatgtac actgtgaggt
     7081 gggaaagctg tatgtttatt ttagcgagta caatcatgcc attgagcatt tcacgaaaac
     7141 tatcgcccag caaggaaagt ccgttgaagc tcattatcat ctggcattgg tgtataatac
     7201 gctgaagcaa tatggtaccg cagccgtaca tgccgatgaa tgtaacaggt taacaaaaaa
     7261 tgacaccgat atgcttttga ctatggctgc cgacgctttg caaaaaaatg aaaaaggtgt
     7321 ggcgatagct cagtatagca gggcattgca ggtaaatcct gcctgtaaag aggcgtatat
     7381 tgggctggca ttgctgtatc gtcaggataa cagtttagca aaggcaatag aggtgcttga
     7441 gcaatgcatc aaggtatatc cgcacgacaa tacaaccgcg atgacattgg cgcacctatg
     7501 ttttgagtat aaaccggcaa aacgccgtaa gtattacatc gatagagcta tcaccttgtg
     7561 caagggtgta gtggagcgtg accccttcca ttgtgaagca tattcattat tatacgatgt
     7621 gtacaaaacg cttcatgatg tgccgctgat ggataactat gcagaggaat acaacaggtg
     7681 ttgtgaaaaa taaatgcgtt caatcaaata ctattgtttt attagcgtaa accaacactt
     7741 tctattctat atgttttttt actgcccgtg aaagaacaag ctattattaa ccctctgtat
     7801 ttatcttgac agtgttattt caagtcagta taattttgga tgtcaggcgt cataaagttt
     7861 taatgggaaa tgatactatg aacgattaca tgtgatggag ataggatata atggacagta
     7921 aagtagaata tataatacaa aaaattgtat caattattgt tgaaaaagct gatcccgcca
     7981 ggattatctt gtttggctcg cgagcaaaag gcaatgcaca taaaggttct gatattgata
     8041 tagcgcttga aggcgagaaa gcacttacat tcagggaaat gcggaagatg aaagaactca
     8101 ttgatgatgt tagtggatta tattctgttg atattatagt gttacgtaag gtagatgata
     8161 cctttaagga tagtataaaa gagtcagggg ttgtaatcta tgaaaaaaag tgagatatat
     8221 ctatctgtta aaaaattgga aaatgctgtt acatcactaa aagaagcggt aagtattgct
     8281 caaaccgatt tagaaaaaga tggtactata cagcgttttg agtttacggt tgagctttta
     8341 tggaaaacat taaaaacgat attattatat aatggtgttg aatgtaataa tcccagagca
     8401 tgtataaaag aggctttcag gaaccgaata atagaagatg atgaaatact ccttgacatg
     8461 ctggaggata ggaatgtgag cgcacatatt ta
//
LOCUS       DAOSOX010000097         8480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8307_length_8480_cov_7.133175, whole genome shotgun sequence.
ACCESSION   DAOSOX010000097 DAOSOX010000000
VERSION     DAOSOX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8307_length_8480_cov_7.133175"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1224
                     /locus_tag="PLX22_07890"
     CDS             439..1224
                     /locus_tag="PLX22_07890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT19860.1"
                     /translation="MDYHTITRCAIDICKQAGAIIVEGYYREHITIDYKSRTNLVTDI
                     DYRSEKFIVHELKKQFPEFDIAAEEGSRERADGEYIWYVDPLDATNNFAHKIPWFCVT
                     IALYSKALEAAVAGVVYEPLRDECFYAWKDGGAYVNEKRIFVSQINDIGISIIATGFP
                     YKKDDPSCNNAKQFNNVLPNVQGIRRMGSAAIDLSYVACGRFDGYWEPLLYPWDMTAG
                     ALLVKEAGGMVTQYNGNTFDPEFPEICATNGLIHQQLLELINK"
     gene            1347..1937
                     /locus_tag="PLX22_07895"
     CDS             1347..1937
                     /locus_tag="PLX22_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19861.1"
                     /translation="MTKKIMCLCFVLVASTLLAQNTQSVDIPVQSLYSNKVRIKDIYF
                     NKKVDLTGKGEVLEVEAILENLTDDPMDVYVNVIATYEKEEKTKSSFEMPIPEKERVR
                     NFVPFPDDIANYQYPDGKGGNKLKAYPKNTKSGIDPSTGKLYHLPVNNIVLVRSTHLS
                     PYKKDFIFFNYVTILVFDNNGTPILREVFELKGKRK"
     gene            complement(2013..2726)
                     /locus_tag="PLX22_07900"
     CDS             complement(2013..2726)
                     /locus_tag="PLX22_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOT19862.1"
                     /translation="MSNPIKESTLERWIQNQMKVWESEKKIIEKEEKPRPFITISRQY
                     GCFANDIAEEIAKALNATTTNNKWSVYDKNLINKIEQQYNISEKIVETIDTRKREEMS
                     ELWRTMLADLPPQAAVFQKLASTIRTIAIHGNAILIGRGSVVITKGLRYGLHLRFVAP
                     LQYRIQKVMENQKIKDRLAAEKLIEEKDKQRHEFMTQYIKFNAYDPASYDITINVAQF
                     TKEQVAQISIAGLKVRKLI"
     gene            2954..>4343
                     /locus_tag="PLX22_07905"
     CDS             2954..>4343
                     /locus_tag="PLX22_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT19863.1"
                     /translation="MKAIIMAGGEGTRLRPLTCNRPKPMIPVMNRPVIEYAIYLLQQH
                     GISDITMSLFYLPENVVNYFGDGTFWDVSIDYSIEELPLGTAGGVKLALKDYDDTFIV
                     LSGDGLYDFNLHDIIEFHKKKRSLFTIALAHVHDPVEYGIVVTHEDGTIDRFLEKPSW
                     GEVFSDTVNTGLYIIEPEIIDKFVPDNDKFDFSYDLFPLLQKNGVPLYGYITDGYWCD
                     IGNLDAYRKVHQDILNGIVKINFPGKKIGENIWAGDDVEIHADAILKGPIVLGNFVNI
                     KKGAEVSEYSVIGDNTVIHENASIRRSVILHNTIVGTKTEIRGAVVGKRNVIEDNVSI
                     YEGAVISDDCQIGNGAKIATGIRIWPEKVIGEGTQLSYDLIWGKTEKKILFTNEGIYG
                     SFNVKITPEFAAKLGSAIGAFLGKNKKVIVSRDTTRASRLIKRALTAGLLSMGVEVYD
                     LEIASLPINKYSV"
     assembly_gap    4344..4443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4444..5592
                     /locus_tag="PLX22_07910"
     CDS             <4444..5592
                     /locus_tag="PLX22_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19864.1"
                     /translation="VEVYDLEIASLPINKYSVVYENADLGIYVQISPHTDLQFNLIKI
                     FNRKGFELSNVERRKIENIFFRGDYPRQSAFEVGRLYYPTHNVESYIMFATQFVNKDS
                     IAQRQYNVIVDCFYGTTTHIFPSLLAAFSCKATVLRGQIREFNSSEDIRIESRDAINS
                     IKNMAKLNSEIGVIVGPHGEQVTIIDEEGTILQWDDIYTLLGLLFIKHRHIQKLFMPV
                     ISSSRLEHIAKDRGVQIERIAKTLSNDEFINIFSRDADTVYPYMIYEYDPMITFLLIL
                     ELAALEGKKLSEIRKGLPCTNMYHTVLPCPVDAKAIIMREITATSNGHIELVDGIRIV
                     YDEGWILILPDPIQPAVHIYCEALTDEARDKIVEEYSVKIKKLTAKYT"
     gene            5604..6719
                     /locus_tag="PLX22_07915"
     CDS             5604..6719
                     /locus_tag="PLX22_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT19865.1"
                     /translation="MKKTPHVNEERLIKTFIDLVKISSPSWEEKGVIDYIVNFSKSAG
                     VECITYPCNNSYNVWIKIPGDNSYVPILFSCHMDTVTPCENIQPLIKQNKITSDGNTI
                     LGADDKSAVAGFLEAILCIKENTLPHGNIEFLFSCAEETGLYGIKGFDLKQVQAKYAF
                     VFDSDGPVGGVVIAAPFHISMKIHVKGKAAHAGMEPEKGISAIRVISEIITNIPHGRI
                     DDETTVNVGTIKGGRATNIVAEEAECTLETRSRKKNKVLTIKKEITSIAKTIALRYKA
                     KITVTSNMDYEGYSINENDSIVSIAMKALQSIGKKPLLAVSGGGSDTNILNARGIKAV
                     NLSSGMRQVHTTKEYIAMQDLFDVPSLVLSIIQAAKK"
     gene            complement(6724..7167)
                     /locus_tag="PLX22_07920"
     CDS             complement(6724..7167)
                     /locus_tag="PLX22_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19866.1"
                     /translation="MIVLTFVIILQISFVIHVYYIISYITKKQDKDFKGFLFTSITNI
                     FLALFLSVFILIFPQELKEINLERILFIESGVVFLIMLSVKAKIASNIYKRAQDPQHF
                     HYSYFGKKVLHPSVVKMSELFTYFLTLPLTVICGAYFIVKLGCGK"
     gene            7319..>8480
                     /locus_tag="PLX22_07925"
     CDS             7319..>8480
                     /locus_tag="PLX22_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT19867.1"
                     /translation="MNQQHTYNLYEIFLNKSSQYPDDIFIVYNDEMIKYSDAFLNVSK
                     IAYALRKAGVANSSNVIMQIGNTPYFIYCFFAILQCNAMPVLVNPMARQYELKYYHDI
                     TTPACIITRSFIADRLTQQGLVSRGCEMIVIDDSSSFTSIKAILDADDYNMDYVPEEK
                     NGAAIIFTAAMDGKALGAVLTHTGIYHSAESVAWMLPHYASMMIAALPLFHAFGLTTS
                     LIIPLISKTEICLVDRFSPKRLLKLLLNRKVRYFVGVPAMYSIMKALFERDIDFSGIS
                     LWVSGGDYLSRELQEWYAEKGIIIRQGYGLTEASPIVSWNMPHIENKICSIGKPMPYN
                     EVMIVDEEGVMQSPLNNGEIIVKGINVINHYYNNEQMTQKYIKNGWLHTGDVG"
BASE COUNT         2777 a         1307 c         1700 g         2596 t
ORIGIN      
        1 ccagggcatt gggattataa tcagtaacta tcacccgcaa tcccattttt tgagctgtct
       61 ggataacggg tacctgtaat aaacctccgc caatcaccat aatcgttttt ttcatggatt
      121 ccccctggac aatagtaaag ttacaacctg ccgggttgcc tctctataga tattattggt
      181 tttttttgca aaaacttgac ggtaattttt acttgcattg tgagggttat tgtcaatact
      241 ataatgtcgt gggctctcac gtaaataatg attgtggcga tagttactgt atatggtgta
      301 gcaaataaaa aattatttga cactgaacag taaaaaggct atcaacaaac attgctgctt
      361 atagatacaa tagctggtat atgagtatat gattattgtg gacatctgat tatagtatgc
      421 ggggagtaga aatgtaccat ggactatcac accattacac gatgtgccat tgatatatgc
      481 aaacaggcag gggcaattat tgttgaagga tattaccgtg agcatataac catagattat
      541 aaaagcagaa caaatctggt tacggatatt gactacaggt ccgaaaaatt tattgtacat
      601 gagcttaaaa aacagtttcc agagtttgat atagctgctg aagaaggaag cagggaacgg
      661 gctgatggtg agtatatatg gtatgttgat cctctggatg caacaaataa ttttgctcat
      721 aaaatcccat ggttttgtgt taccattgca ctgtattcaa aagcgctgga agcggcggtt
      781 gccggcgttg tgtatgaacc gctcagggat gaatgctttt atgcgtggaa agacggcgga
      841 gcgtatgtaa atgaaaaacg tatctttgta tcgcagataa acgatatagg gatatcgatc
      901 attgctaccg gctttcccta taaaaaggac gatccttcat gcaataatgc aaagcaattt
      961 aataatgtat tgccaaacgt tcagggtatt cgtcgtatgg gctcggctgc aattgatctg
     1021 tcgtatgttg catgtggaag atttgacggc tactgggaac cactgttata tccatgggat
     1081 atgactgctg gcgctttact ggttaaggaa gctggcggca tggtgacaca gtataatggc
     1141 aacacatttg atccggaatt tcctgaaata tgtgctacta acgggctgat tcatcaacag
     1201 ttgctggagc tcatcaataa atagggtatg gtattctgct caattttatt aaaaaaatgc
     1261 ccgataaatg ggtaaggata attatagtaa aactatctac cccctatggg ttgtgatggt
     1321 tattaatatg atagcaggtg gcttatatga caaaaaaaat tatgtgtcta tgctttgtac
     1381 tggtggcttc cacattgctt gctcagaaca ctcagagtgt tgatattcca gtacaatcct
     1441 tatatagcaa taaggtgcgg ataaaagata tttattttaa taaaaaggtt gatctcaccg
     1501 gtaagggcga ggtgctggag gttgaagcaa tactggaaaa cttaactgat gatcctatgg
     1561 atgtgtatgt caatgttatt gctacctatg aaaaagagga aaaaacaaaa tcaagttttg
     1621 agatgcccat acctgaaaaa gaaagagtta gaaattttgt tccttttccc gatgatatag
     1681 caaattacca gtatcctgac gggaaagggg gtaataagct taaagcatat ccaaagaata
     1741 caaaatccgg gattgatcca tcaacaggga agctctatca tctaccggta aataatatag
     1801 tacttgttag atcaacccat ttatcgccct ataagaagga ttttatcttt tttaattatg
     1861 ttaccattct ggtttttgat aacaatggta cccctatctt acgtgaagta tttgaattga
     1921 aagggaaaag aaagtagttt gctaattcag ataagataac agttgttaaa aataccaata
     1981 aaaaggagtt gtccacaggg caaccccttt tgctatataa gctttcgcac ttttaatcct
     2041 gctatactaa tctgggctac ctgctctttt gtaaactgag caacattgat tgtaatatca
     2101 tacgatgccg ggtcataagc attaaatttt atgtattgtg tcataaactc atgtcgctgt
     2161 ttatcttttt cttctattag tttttcagca gcaagcctat cttttatttt ttggttttcc
     2221 ataacctttt gtatgcggta ttgcagggga gcaacaaagc gcagatgcaa gccatatcgt
     2281 agaccttttg taattactac agaaccacgc cctattaaaa tagcatttcc gtgtattgct
     2341 atggtacgta ttgtgctggc taatttctga aaaactgctg cctgtggtgg caaatctgca
     2401 agcattgtac gccataattc ggacatctct tcgcgttttc gggtatcaat agtttcaact
     2461 attttttcag atatattata ctgttgttca attttattaa tgagattttt atcatagact
     2521 gaccatttgt tattggtagt tgtggcattc agagcttttg ctatttcttc ggcaatatca
     2581 ttggcaaaac atccatactg acgggagata gtaataaaag gacgtggttt ttcctctttt
     2641 tcaattatct ttttttcaga ttcccagacc ttcatttgat tctggatcca tcgttctaat
     2701 gttgattctt tgattggatt gctcataatt aactcctata attgcaatag taaaataaat
     2761 aatgatgaca aaattaatcc aactatctaa cagcatatta ttttttcaat tattttactt
     2821 tgcataagat gcaattttta tatatatgac caaaaattat tttgtaaata aaacagctat
     2881 tgtatattta acttgacctg atgctcaaat atctggttat atgaaaatat agattttaaa
     2941 ggaaaggata agcatgaaag caatcatcat ggctggagga gaggggacaa gactgaggcc
     3001 cttaacctgt aacaggccga aaccaatgat acctgttatg aaccgcccgg ttattgagta
     3061 tgcaatatat ttattgcagc aacatggtat ttcagatatt accatgagtc ttttttacct
     3121 tcctgagaat gttgttaact attttggtga tggcaccttc tgggatgtgt caatagatta
     3181 ttctattgaa gaattaccat tggggacagc aggaggggtg aaattagcat tgaaggatta
     3241 tgatgataca tttattgtgc tgagtggtga tggactctat gattttaatt tacatgatat
     3301 tatagaattc cataagaaga aaagatcatt gtttacgata gctctggcac atgtgcatga
     3361 ccctgttgaa tatggcattg ttgttaccca tgaagatggc actattgaca ggtttttaga
     3421 aaaaccatcg tggggcgaag tattcagtga cacagtaaac actggattgt atatcattga
     3481 accagagatt attgataaat ttgtgccaga taatgataaa tttgattttt catatgattt
     3541 atttccttta ctacagaaga atggagtccc tttatatggc tatataaccg atgggtattg
     3601 gtgtgatata ggtaatctgg acgcgtatcg caaagtacat caggatatac tcaatggaat
     3661 tgtaaagatt aattttccag gtaaaaaaat aggtgaaaac atctgggctg gtgatgatgt
     3721 tgaaattcat gcagatgcaa tactaaaggg acctattgtg ttaggaaatt ttgttaacat
     3781 taaaaaaggt gccgaggtat cagagtacag tgttattggc gacaatacag ttatacatga
     3841 aaacgcttca atacgaagaa gtgtgatatt acataataca atagtgggta ccaaaactga
     3901 gatacgggga gctgtggttg gcaaacgcaa tgttattgaa gataatgtct caatttatga
     3961 aggtgctgtt ataagtgatg attgtcagat aggaaatggt gctaaaatag ccaccggtat
     4021 cagaatatgg cctgaaaagg ttattggtga aggtacccaa ttgagttatg acctgatctg
     4081 gggaaaaacg gaaaaaaaga tattattcac caatgaagga atatatggct cattcaatgt
     4141 aaaaattaca cctgaatttg ctgcaaaatt agggtccgct attggagctt ttttggggaa
     4201 aaacaagaag gtcattgtca gcagggatac aacacgggca tcacgattaa taaaaagagc
     4261 gttgactgca ggattactat ccatgggggt agaagtatat gaccttgaga tagcaagttt
     4321 accaataaat aaatattcgg tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnngtagaag tatatgacct tgagatagca agtttaccaa taaataaata ttcggtggtt
     4501 tatgaaaatg ctgatcttgg tatatacgtt caaatatcac cccataccga tttgcaattt
     4561 aaccttataa aaatatttaa tagaaagggg tttgagttaa gcaatgttga acgacgaaaa
     4621 atagaaaata tatttttccg tggtgattat ccacgccaga gtgcttttga agtaggcagg
     4681 ttatattatc ccactcataa tgttgaatca tatataatgt ttgctacaca atttgtaaac
     4741 aaagatagta tagcacaacg acagtataat gtaatcgttg attgttttta tggaactaca
     4801 acacatatat ttccatctct tttagcagca ttttcatgca aggcaacagt attgcggggc
     4861 cagatacgag agttcaatag ttccgaagac atacgaattg aaagtagaga tgcaataaat
     4921 agcataaaaa atatggcaaa attgaatagc gagataggag ttattgtagg ccctcatggc
     4981 gaacaggtaa caattattga tgaagaggga acaatcttgc agtgggatga tatttataca
     5041 ttattgggac tattatttat taaacacaga cacattcaaa aactattcat gccggttata
     5101 tcatcgtcac gtctggagca cattgcaaaa gatagaggtg tacaaataga acgaatagct
     5161 aagacactat ccaatgatga atttattaat attttttcac gagatgcaga tacggtttac
     5221 ccgtacatga tatatgaata tgatcctatg ataacatttt tattaatttt agaactggca
     5281 gcattagaag gtaaaaaatt atctgagata aggaaaggat tgccctgtac taatatgtac
     5341 catactgtgt taccatgccc ggttgatgct aaagcaataa ttatgcgtga gattacagca
     5401 acttctaatg gtcatattga actggtagac ggtataagga tagtgtatga tgaaggatgg
     5461 attttaatct tacccgatcc aatccagcct gctgtacata tatactgtga ggcgttgaca
     5521 gatgaggcgc gggataaaat agttgaagaa tacagtgtta aaataaaaaa gttaaccgca
     5581 aaatatacgt gaggcatatg aatatgaaaa agacaccaca tgttaatgaa gaaagactga
     5641 ttaaaacttt tattgacctt gtcaaaattt cttctccgtc atgggaggaa aaaggtgtta
     5701 ttgattatat tgttaatttc agtaaatcag ctggtgttga atgcataacc tatccctgta
     5761 ataattcgta taatgtgtgg ataaagattc cgggtgacaa tagttatgtt cccatccttt
     5821 tttcatgcca tatggatacc gttaccccct gtgaaaatat acaaccttta ataaaacaaa
     5881 ataaaattac ttcagatggg aatacaattt taggtgccga tgataaatcg gctgttgcag
     5941 gatttttgga agcaatacta tgtattaaag aaaatacact accccatggg aatattgaat
     6001 ttttattctc atgtgctgaa gaaaccggat tatatggaat aaaaggtttt gatctaaaac
     6061 aggtgcaggc aaaatatgca tttgtttttg acagtgatgg accggtaggg ggggtggtta
     6121 ttgcagcacc atttcacatc agtatgaaga tacatgttaa agggaaagct gctcatgcag
     6181 ggatggaacc ggaaaagggt atcagcgcaa tacgggtaat atcagaaata atcactaaca
     6241 tcccccatgg aagaattgat gatgaaacta ccgtcaatgt tggaactata aaaggtgggc
     6301 gagcaaccaa cattgttgca gaagaagctg aatgtactct tgagactcgt tcacgtaaaa
     6361 aaaataaggt attgaccata aaaaaagaaa ttacttctat tgctaaaaca attgcactac
     6421 gatataaagc aaagattacg gtcacaagta atatggatta tgagggatat agtataaacg
     6481 aaaatgacag tattgtttcc atagctatga aggcattaca aagcatcggg aaaaagccac
     6541 ttttagctgt ttcaggggga ggtagtgata ccaatatatt aaatgcacgt ggcataaaag
     6601 cagtaaacct ttcttctggc atgcggcagg tgcatactac aaaagaatat atcgccatgc
     6661 aggatttgtt tgatgttcct tcattggtac tgtctataat tcaggctgct aaaaaatgat
     6721 gatctatttg ccacagccta attttacaat aaaataagca ccacatatca ccgttagagg
     6781 gagtgttaaa aagtatgtaa aaagttctga catcttaact accgatggat gtaaaacttt
     6841 ttttccaaaa tatgaataat ggaaatgctg gggatcctga gcacgtttat aaatatttga
     6901 agcaattttt gctttaacgg aaagcattat taaaaaaaca acacctgatt cgataaaaag
     6961 aatacgttcc aggttaattt ctttaagttc ctgtggaaaa attaagataa aaacagacag
     7021 gaacagtgct aaaaatatat ttgttatgga cgtaaaaaga aagcccttga aatccttatc
     7081 ctgcttcttt gtaatataac ttatgatata atatacatga attacaaatg atatttgaag
     7141 gatgatgaca aacgttaaaa caatcataaa ttagcggctc ttctgtgata tttttgtatt
     7201 ataatatatt tactgtataa gtcaagaaaa tcattattat aactgataga tttacttgta
     7261 ttttttcaaa gaatcatgta caataactat cgccaaaaat ataatggaaa ttacttgtat
     7321 gaaccagcag catacttata acctttatga gattttttta aacaaaagtt cacaatatcc
     7381 tgatgacatt tttattgtgt ataatgatga aatgatcaaa tacagtgatg cttttttgaa
     7441 cgttagcaag attgcctatg ccctccgcaa ggcaggagtt gccaatagct caaatgttat
     7501 catgcagata ggaaatacac catattttat ctattgtttt ttcgcaattt tgcaatgtaa
     7561 tgcgatgcct gttttagtaa atccaatggc gcgccaatat gagcttaagt attaccatga
     7621 catcactacg cctgcatgta tcattacccg gagtttcatt gcggataggt taacacagca
     7681 aggtcttgta tccaggggat gtgaaatgat cgtcattgac gatagtagta gctttacttc
     7741 aataaaagca atacttgatg ctgatgatta taacatggat tatgttccag aagaaaaaaa
     7801 tggtgctgca ataattttta ctgctgccat ggatgggaaa gcattagggg ctgtgttaac
     7861 tcataccggg atatatcaca gtgcagaaag tgttgcgtgg atgcttcctc actatgcctc
     7921 aatgatgata gcagcgctac cattgtttca tgcttttgga cttactacct cactcataat
     7981 accgctgata tcaaagactg aaatatgtct tgttgacagg ttctcaccta aaagactatt
     8041 aaaattattg ttaaacagga aagttcgtta ttttgtaggt gtgcccgcca tgtatagtat
     8101 tatgaaagcg ctttttgaac gtgatataga cttttcgggt attagcttat gggtaagtgg
     8161 cggagattat ctttcacgtg agcttcagga gtggtatgct gaaaaaggca ttattatacg
     8221 gcagggctat ggattaactg aagcctctcc aattgttagc tggaatatgc ctcatattga
     8281 aaataaaata tgctcaattg gaaaaccaat gccctataat gaagtcatga tagtagatga
     8341 agaaggagtg atgcaatcac cgcttaataa tggtgaaatc attgttaagg ggattaatgt
     8401 tatcaaccat tattataaca acgagcagat gacacaaaaa tatatcaaaa atgggtggct
     8461 ccataccgga gatgtagggt
//
LOCUS       DAOSOX010000098         8463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8327_length_8463_cov_7.825167, whole genome shotgun sequence.
ACCESSION   DAOSOX010000098 DAOSOX010000000
VERSION     DAOSOX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8327_length_8463_cov_7.825167"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..304
                     /locus_tag="PLX22_07930"
     CDS             59..304
                     /locus_tag="PLX22_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT19868.1"
                     /translation="MIGLVAASHAIQSQRDNIASAKESVRVAVLQFKNGMIDNTELLN
                     ANVELSNATTMYIQALYYFQVSKAKLNRALGYDYFSF"
     gene            638..1459
                     /locus_tag="PLX22_07935"
     CDS             638..1459
                     /locus_tag="PLX22_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT19869.1"
                     /translation="MMTLEESYKLLQVSYNASDAEITKAFKKLAFMYHPDKNPGNVDW
                     ANKAMASINVAYNTIITHRFKENTVEIQNDKPPKKETTFKKDDILRDDLLTQYFIQYR
                     EKAKDALYQYFQYNLYNLARRDSPVNSDIFRKIVLQLRRSYHGIDNLCSYTNDHEFLE
                     HFNLFKELLFTFYKSSECLNIIDSYANILDVEAFRIYRQGDDFLLRAQKEIFYNRHNR
                     GSFQMEQAITDLIKSIQILQLAINRFPQSSWIVESQIKLDHALTIEHYLKLFFER"
     gene            1462..2022
                     /locus_tag="PLX22_07940"
     CDS             1462..2022
                     /locus_tag="PLX22_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT19870.1"
                     /translation="MDIITTLGIAVALAMDAFSVSIAAGLYIYKPFYRYYFRIAFHFG
                     LFQCAMPIIGYFGGIAIEPIIKNFDHWIAFALLSFIGIRMIKGSFNNDGSAFNGDPSK
                     GLPLILLSLATSIDALAVGLSLGVLQKPILLPSIIIGITCAVFSVFGVFLGKRASSLL
                     GKRTELIGGIILIFIGIRIIISHMSN"
     gene            2222..2932
                     /locus_tag="PLX22_07945"
     CDS             2222..2932
                     /locus_tag="PLX22_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19871.1"
                     /translation="MNTKIIATKLTPFLIAGLFVLISIHADAEDYQYHYFQKEKDEFV
                     PFSDYIPKVRMHYKVVPHYVEDFYELYGMKLYYDENSLRKNIDRLKIALTCKFRHPSQ
                     ALVKIQTEDEYYKYRNLMFMHITMLILRDYLKIATRYDKQHIYFYDVNYAKDIRESLK
                     IAETMYKEALPYWYQAKKYATIASTVKITTNLGYIETERVKIIRGDVDFEKIINDHVS
                     RVKKKQNSVDALLATYQK"
     gene            2937..3437
                     /locus_tag="PLX22_07950"
     CDS             2937..3437
                     /locus_tag="PLX22_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT19872.1"
                     /translation="MDILKAIKTRRSIRHYKALPIPDNIINDIIEAGIWAPSGLNNQP
                     WRFILIRSHNKKEQLAQYTSYSRIIKESQVCICVYYHIPSGYNRDKDIMGIGACIQNM
                     LLAVHAKGLGAVWLGQILNQKDKVSTLCELNNDYELMAVIALGYPNEKGHSTRHPLES
                     FIIKEL"
     gene            complement(3456..3908)
                     /locus_tag="PLX22_07955"
     CDS             complement(3456..3908)
                     /locus_tag="PLX22_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOT19873.1"
                     /translation="MDTFKTYTTLFGLDEQFTIDELSNAYRTLAMLYHPDISRDANAL
                     QKMQLINTAYDYLKNFVDSSIKADSTKEYEKIDEIYSLYKKAFTILQNSFYGYYSDGS
                     GFTGDAKYLKSELIKAKELFATIIKDYPYNQWVDDAIDKINSINKWVQ"
     gene            complement(3912..4997)
                     /gene="ychF"
                     /locus_tag="PLX22_07960"
     CDS             complement(3912..4997)
                     /gene="ychF"
                     /locus_tag="PLX22_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT19874.1"
                     /translation="MGFTCGIIGLPNVGKSTLFNALTGAHAPMENYPFCTIEPNKGIV
                     PVPDPQLYTIANILEKKDPIPTRIEFVDVAGLVKGASKGEGLGNMFLSHIRNVDAIIH
                     VIRCFTDKDVVHVEGDIQPLRDIEIIHMELLLSDIEVLTRAKEKIEKKARSGDKENAQ
                     RLQIIDKLFTHINDGVMLNRVPLTQEELSLCNEYGLISAKPMIICANVDEYGDSNHAF
                     NSLQEYARKHGLGIVAISAKVEEEIAELPLLEQQEYRKALGVDRSALERLIITAYSML
                     DLITYYTITTQLQAWTIKNGTTAQKASGIIHSDFEKGFIRAEVFQYDDLMHYKSLHAI
                     REHGLLRSEGRDYIVKDRDIIHFLFSV"
     gene            5128..6495
                     /locus_tag="PLX22_07965"
     CDS             5128..6495
                     /locus_tag="PLX22_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT19875.1"
                     /translation="MISSLLLSLCIVSSLFLCVAIATINIKEGRHSVPLLVFALLLGI
                     VTLIHPSTIFPSIIISCYPGVFAYYVHAIAKQLSKKDIVISGISIVTLLVSVILPLHI
                     QPAIIAIFQVYYSFTMKNSVPSRFGLKLFRATLFIESGILGILTLFANTHITTLTIMG
                     FITFFLTITIYDILKRSQLALYKMRSLAHINANLTKLTRLLKQRNDQYVTMLNKKDND
                     IQMLSKYSTMAEITASIAHEIAQPLTGIKSIAQNMMDDINLDDFNYLEALSELQKICT
                     MVDKATFIMDHIRSFNTNNHIYKLIDINSIIMNAVTLMQNQLKKHNIDIILLLNESIP
                     RIYGNAISIEQILINLITNAKDAIIEKTNKTNPDYSGRIMIKTDHHDDHVFIIIEDNG
                     IGIQPDIQKHIWSPFFTTKRYSQSLGIGLSISKKIINDHNGEIFINSKPEEGSVFTIK
                     FSLIK"
     gene            6594..8294
                     /locus_tag="PLX22_07970"
     CDS             6594..8294
                     /locus_tag="PLX22_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HOT19876.1"
                     /translation="MNRLLLRGFTFGFVVLFLLILKQYSPGLEVAIDEIRTKPVKFIN
                     YQGPYKKSDSPQAIEQIGRALARTPSNNISMFSNKYSMIHAISKNEPNKFSATIFSIN
                     KQARVGHIAIIRKILSSYLQQKYGYTKHHAEAIALFLTYYNAAYRGDIDYFSLKYKSI
                     VMIYINKYNAGISTKYYEWPGNTRMLIPLTDKPQKGKLDAIVVDVISNNDIVKEVRKD
                     DKNIIARKDMVDLKKQIITNEKTEVEQSKTILNEDIKKLSDTKKALENDKTELQQKEK
                     EIRKTKEVLKTISDSEKQKTMQKEIETKEKQLDYDKQEVKKKEEKIEKKESEISKQKD
                     QISTKDKKLEQKQKDLEKEEEQIAFDEAKQTSNNKQELIAKLQAKEKQLDEREDKLRT
                     KEVDKSIYADKLFYLKIKEYLQDGHYNNEMIMIDPSLRKILFKSPELNICGSKYDIFS
                     KGVVVITHKGSHSIGHRLTLLDRNTLEPIIYGNDNIFWRSFIIIKDDTIFAIVIDNGK
                     FYLGKFDTNLVLISKSSEEVNENTFISFWNNYIYINSVDKTIMVLDNKTLKLIDTIKP
                     "
     gene            complement(8349..>8463)
                     /locus_tag="PLX22_07975"
     rRNA            complement(8349..>8463)
                     /locus_tag="PLX22_07975"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2987 a         1448 c         1356 g         2672 t
ORIGIN      
        1 caatacagct ttattatgga gtgctacttg agcaggaaat ggtaaaattc aggatggatt
       61 gatagggctt gtagcggcat cacacgccat tcaatcccaa agggataata ttgcatctgc
      121 gaaggaatca gtccgtgttg ccgttttgca atttaaaaat ggtatgatag ataatactga
      181 acttttaaat gcaaatgtag agttaagcaa tgcaaccaca atgtatattc aggctctcta
      241 ttattttcag gtttccaaag caaaacttaa tcgtgcactt ggttatgatt attttagctt
      301 ttgaaatgga aattaaaatg ctgttgtact ctcgtttcaa gattcttttc ttattggcag
      361 ctttatggtg gcagttgcat ttattttatc atttttcctg ccaggcaaat cagccaggag
      421 ccgtataacc gttaaaaacg aaggtgagat tattgaagag attgcaacaa tagattaatc
      481 cctgacacgg tagtagtaaa atcaccgcgt tatgatattc gcaaacagta aaagcataat
      541 cttaattttt ataaaaatgt aattgcattg ttatgcttat attttataca cttatcagtt
      601 tactaaaact ggtacgttta ctttccatta tataataatg atgacactgg aagaatcata
      661 taaactttta caggtatcat acaatgcaag cgatgctgaa ataacaaaag cattcaagaa
      721 gcttgcattt atgtatcatc ctgataaaaa tcctggcaat gttgactggg ctaataaagc
      781 tatggcaagc attaatgttg catacaatac cataattaca catcgtttta aagaaaatac
      841 ggttgagatt caaaacgata aacccccaaa aaaagaaacc acatttaaaa aagacgatat
      901 tttgcgtgat gatctattaa cccaatattt tatacaatac cgcgaaaaag caaaagatgc
      961 actgtaccag tattttcaat ataaccttta caacttagcg cgtcgtgaca gtccggtaaa
     1021 ttccgatata tttagaaaaa ttgtattgca gcttcgccgc agctaccatg ggattgataa
     1081 tctatgcagc tataccaatg accatgaatt tttggagcat ttcaaccttt tcaaagagtt
     1141 gctttttact ttttataaat cttcagaatg tctcaatatt attgattcct atgccaatat
     1201 actggatgta gaagccttca gaatttaccg gcagggggat gattttcttt tgcgcgctca
     1261 aaaagaaatt ttttacaata ggcataaccg aggctctttt caaatggagc aggcaataac
     1321 cgatcttata aaatcaattc aaattttaca attagcaatc aatcgctttc cacaatcttc
     1381 atggattgtt gaatcgcaaa taaagcttga tcatgcatta actattgaac attacttaaa
     1441 actttttttt gagcgatagt tatggacatt ataactacat tagggatagc tgtggcactg
     1501 gcgatggatg cattctcagt ttccatagcc gcaggtttat atatttataa accattttat
     1561 cgctattatt tcagaattgc tttccatttt ggtttattcc aatgcgcgat gcctattatt
     1621 ggttattttg gtggtatcgc tattgaacca attataaaga attttgatca ctggattgca
     1681 tttgcgctac tatcatttat tggaatccgt atgattaaag gatcttttaa taacgatgga
     1741 agcgccttca acggtgaccc atcaaaggga ttgcccctta tcctcttatc gcttgcgaca
     1801 agcattgatg ccctggcagt agggttaagt ttgggagtat tgcaaaaacc aatactactg
     1861 cccagtatca ttattggtat tacctgtgcg gttttttcgg ttttcggcgt atttcttggt
     1921 aaaagagcaa gcagtttgtt aggcaaaagg acagaactga ttggtggtat aatcctcatt
     1981 tttattggaa tcagaatcat tatatcacac atgagtaatt gatttaccta ttgtttcttc
     2041 atttccgata aatataatag atatcacatc gtgaggcgca aacgattttc accatagtgt
     2101 acagtataca gttttcattg atgcacaata gcatgtttaa tatctgttta atgtatgttt
     2161 aatgatagag agaattatgc atatgcacta tatcttaaat aattttttgt atagcattac
     2221 tatgaataca aaaataattg ccacaaagct gacaccattt ttaatagctg gattgtttgt
     2281 attgataagc attcatgcag atgctgaaga ctatcagtat cactattttc aaaaggaaaa
     2341 agatgaattt gtaccttttt cagattatat tccaaaagtc aggatgcatt ataaagtagt
     2401 tcctcattac gttgaagatt tttatgaatt atatgggatg aagctatact atgatgaaaa
     2461 ttctttacgc aaaaatattg acagacttaa aatagcatta acctgtaaat tccgccatcc
     2521 atcacaggcg ctggtaaaaa tacaaactga agatgaatac tataaatatc gtaatttaat
     2581 gtttatgcat attaccatgc tgatactacg tgattatcta aaaatcgcta cccggtatga
     2641 taaacaacat atatattttt atgatgtaaa ttatgctaaa gatattcggg aaagtcttaa
     2701 aatagctgaa acaatgtata aagaagcgtt gccgtattgg tatcaggcaa aaaaatatgc
     2761 caccatagca agtactgtta aaataaccac caatttaggc tatattgaaa cggaacgagt
     2821 aaaaataatt cgcggtgatg ttgattttga aaaaattatt aatgatcacg ttagtcgggt
     2881 taaaaaaaag caaaatagtg ttgacgctct tttagctacg tatcaaaagt aattgtatgg
     2941 atatactcaa agctattaaa actcgacgct ctatacgtca ttacaaagct ttgccaatac
     3001 cagacaacat catcaatgat attattgaag ctggtatatg ggcaccttcg ggcttaaaca
     3061 accagccctg gcgctttata ttgatccgct cacacaataa aaaagaacaa ttggcacaat
     3121 atacatcata ttcccgtatt atcaaagaat cacaggtgtg tatatgcgta tactatcaca
     3181 ttccctcagg atataaccgg gataaggata ttatgggaat tggcgcatgc attcaaaata
     3241 tgttgcttgc tgtacatgcc aaaggattag gcgcagtgtg gttaggccaa atacttaatc
     3301 aaaaggataa ggttagcact ctgtgtgagc ttaataacga ttatgaacta atggctgtca
     3361 ttgccttagg ataccccaat gaaaaaggac attccacacg ccatccatta gaaagtttta
     3421 taatcaaaga attgtaattg tttattgtat tggatctatt ggacccattt attgatacta
     3481 tttattttat caatagcatc atccacccat tgattatagg gatagtcctt gatgatggtt
     3541 gcaaagagct cttttgcttt tatcagttca ctttttaaat acttcgcatc gccagtaaaa
     3601 ccgctcccat cagagtaata gccataaaag gaattttgta gaatagtaaa tgctttttta
     3661 tacagtgaat atatctcatc gattttttca tattctttgg tactatctgc ttttatagaa
     3721 ctatcgacaa aattttttaa ataatcatag gcagtgttta taagctgcat tttttgcaat
     3781 gcattggcat ctctgctaat atctgggtga tacaacatgg ctaatgttct gtacgcgttt
     3841 gaaagctcat cgattgtaaa ctgttcatcc aacccaaata gcgttgtgta ggttttaaat
     3901 gtatccataa tttaaacact gaacaaaaag tgaatgatgt ccctatcctt tacaatataa
     3961 tcacgccctt cacttcgtag caatccatgc tctcttatgg catgcaatga cttatagtgc
     4021 ataagatcat catactgaaa aacttctgca cggataaatc ctttttcaaa atcactgtgg
     4081 ataatcccgc ttgctttttg agcagtagta ccatttttta tggtccacgc ctgcaactgt
     4141 gtggttatag tataataggt aataagatca agcattgagt atgcagttat aatgagcctt
     4201 tccagcgcag atctgtccac acctaacgct tttcgatact cctgctgctc cagcaacggc
     4261 agttctgcaa tttcttcttc aacctttgca ctgattgcaa caattcccaa tccatgtttc
     4321 cttgcatatt cctgcaatga attaaatgca tggttactat cgccatactc atcaacattt
     4381 gcacatataa tcattggttt tgcagaaata agaccatatt cattgcaaag cgatagttcc
     4441 tcctgagtta acggtaccct gttaagcatg acaccatcgt taatgtgggt aaacagctta
     4501 tcaatgattt gcagccgctg agcattttct ttgtcaccag atcttgcttt tttttcaatt
     4561 ttttcctttg cacgtgtaag aacttctata tcagaaagca acaattccat atggataatt
     4621 tcaatatcac gaagaggttg gatatcccct tcaacatgaa caacatcctt atcagtaaaa
     4681 caacgaatta catgaattat cgcatccaca ttccgtatat ggcttaaaaa catgttgccc
     4741 agtccctcac ctttcgacgc ccctttaacc aatccggcaa catcaacaaa ttcaatcctc
     4801 gttggaatag ggtctttttt ttctagaata tttgctatag tatacagttg cgggtcaggt
     4861 accggtacaa ttcctttatt tggttcaata gtgcaaaagg gataattttc cattggtgca
     4921 tgcgcacccg ttaatgcgtt aaacagcgtt gacttaccaa catttggcaa acctattatg
     4981 ccacaggtaa aacccatgcc tggctcctta caataaacaa attttttttg aaaaatgttg
     5041 acatatcatt caataattac cgagatgtag caatggcaga tagttatggc aattttacac
     5101 tactggtaaa aaaaatcaaa tagtattatg atatcttcac ttttattatc attatgtata
     5161 gtatcctcac tattcttatg tgttgctatc gctaccatta atattaagga gggacggcat
     5221 agtgtcccat tattagtatt tgcattatta ctgggtattg taacactgat acatccttca
     5281 actatttttc catcaattat tatttcatgc tatcccggtg tttttgcata ctacgtccat
     5341 gctattgcca aacaactttc taaaaaagat atcgttatat caggcatatc gatagtaaca
     5401 ttactggtat cagtaatttt gccactccat attcaaccag ctattattgc tatttttcag
     5461 gtatactatt catttaccat gaaaaattct gttccatcca gatttggcct aaaattattt
     5521 cgagcaacat tgttcataga atcaggtatt ctgggtatac taacattgtt tgcaaacacc
     5581 catataacaa cactaacaat aatgggattt ataacatttt ttttgaccat tacaatatac
     5641 gatatattga aacgttcaca attagcattg tataaaatgc gttcccttgc ccatattaat
     5701 gcaaacttga ccaaattaac acgactttta aaacagcgaa atgatcaata tgtaaccatg
     5761 ctcaataaaa aagataatga tatccagatg ctttcaaaat attctacaat ggctgaaatt
     5821 actgcaagca tagctcatga gatagcacaa ccgttaacgg gaataaaaag cattgctcaa
     5881 aatatgatgg atgatattaa tctggatgat tttaactatt tagaggcact atcggaacta
     5941 caaaagatat gcaccatggt tgataaagct acgttcatta tggatcatat acgcagcttc
     6001 aataccaata atcatatcta taaacttatt gatataaaca gcatcatcat gaatgccgta
     6061 actcttatgc aaaatcaatt gaaaaagcat aatatagata ttatacttct tcttaacgaa
     6121 tcaatcccgc gaatttatgg taatgccatt agtatagaac aaattttaat taatttaatt
     6181 accaatgcta aagatgcaat aattgaaaaa acaaacaaaa ccaatcctga ttactccggt
     6241 agaataatga tcaagactga tcaccatgat gatcatgttt ttattataat cgaagataat
     6301 ggtataggta ttcaacctga tatacaaaaa catatctggt caccattttt tactacaaag
     6361 agatatagtc agagtcttgg tattggatta tcaataagca aaaaaataat aaatgatcat
     6421 aatggagaga tatttatcaa ttcaaaacca gaagaaggca gcgtttttac tatcaaattt
     6481 tctttaataa aataagaaag cagttttttc tgatgtttta tatatataaa aacttgttgt
     6541 attaatacca tatagtacag tatactcatg tgcatccagt aaagagaggg tatatgaaca
     6601 ggttattatt acgaggattt acatttggtt ttgtcgtcct tttcttatta attttaaaac
     6661 aatattcacc tggattagaa gttgctattg atgaaatacg aacaaaacct gtaaaattta
     6721 tcaattatca ggggccatac aaaaagagtg attcaccgca agctatcgaa caaataggca
     6781 gggctttagc cagaacacca tcgaataata tatccatgtt tagcaataaa tattcaatga
     6841 tacatgctat atcaaaaaat gaacccaata aattttcagc gacgatattc tctataaata
     6901 aacaggcacg agtgggacat attgctatta ttagaaaaat cttatcttcg tatctacagc
     6961 aaaagtatgg ttacactaaa catcatgctg aagcaattgc tctttttctt acatattaca
     7021 atgcagccta cagaggagat attgattatt tttcattaaa atataaaagt atcgtaatga
     7081 tctatataaa caaatataat gcaggaattt caacaaagta ttatgaatgg ccgggaaata
     7141 ctagaatgct tataccatta actgacaaac cacaaaaggg aaaacttgat gctatagttg
     7201 ttgatgtaat ttccaacaac gatattgtaa aagaggtaag gaaagatgat aaaaatatca
     7261 ttgcccgtaa agacatggtg gaccttaaaa aacaaattat tacaaatgaa aaaactgaag
     7321 ttgagcaaag taaaaccata ttaaatgaag atatcaaaaa actgtcagat acgaaaaaag
     7381 ctcttgaaaa tgataaaaca gaattacaac aaaaagaaaa agaaattaga aaaacaaaag
     7441 aagtattaaa aacaatatca gactccgaaa aacaaaaaac catgcaaaaa gaaattgaaa
     7501 ctaaagaaaa acaacttgat tacgataaac aagaagtaaa gaagaaagaa gaaaaaattg
     7561 aaaaaaaaga atcagaaata tctaaacaga aagatcaaat ttctacaaaa gataaaaaat
     7621 tagaacaaaa acaaaaagat ttggaaaaag aagaagaaca aatagctttt gatgaagcca
     7681 aacaaacatc aaacaataaa caagaattaa ttgctaaact ccaggcaaaa gaaaaacagc
     7741 tggatgaaag agaagataaa ctgcgtacaa aagaagttga taaaagcatc tatgctgata
     7801 agctctttta tcttaaaatt aaagaatatc tgcaggatgg tcattataat aatgaaatga
     7861 taatgataga tccatcatta agaaaaattt tattcaaatc gccagaatta aatatttgtg
     7921 gcagcaaata tgatatcttt tcaaaaggtg ttgttgttat tactcataaa ggtagtcatt
     7981 ccattggaca ccgtcttaca ttgctagatc gtaatacgct tgaaccaatc atttatggca
     8041 acgataacat tttctggcgc agtttcatca ttataaaaga tgatactatt tttgcaattg
     8101 tcattgataa tggaaaattt tatctaggca agtttgatac taatcttgtc ttaatatcaa
     8161 aatcttcaga agaagttaat gaaaacactt ttatcagctt ttggaataac tatatataca
     8221 taaacagtgt agataaaact attatggtac tggataacaa aacattaaaa ttaattgata
     8281 caataaaacc gtaatatgca cattaaataa tgaaaaagcc cctcttttga agaggggctt
     8341 tttataatat ggtcaagcct cacgactgat tagtactggt tagctgagca cattactgcg
     8401 cttacacctc cagcctatca acctcgtaat cttcgaggag tcttcagtga cctcaagggt
     8461 cag
//
LOCUS       DAOSOX010000099         8437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8364_length_8437_cov_9.152827, whole genome shotgun sequence.
ACCESSION   DAOSOX010000099 DAOSOX010000000
VERSION     DAOSOX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8364_length_8437_cov_9.152827"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1405)
                     /locus_tag="PLX22_07980"
     CDS             complement(23..1405)
                     /locus_tag="PLX22_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOT19877.1"
                     /translation="MVKPVILDFDIVTHNQNSARQSEPEQSFISFLDGILQKKEVEET
                     VNSKNVITEFEESNHTKNDNQDKYFADMANKNELSTEKAAKHVSSFNDRRLEKEKADK
                     EKLQLHNEALSQFLTISNSNTINSRATLTKNKDNPVRIPYRDMNISPRRLLHGLDTSS
                     AQRNNTSTNDLFSTNTLSGTKTGNSQSLHKVIENMQKIINDEMVNNSKRGDIKKLIAN
                     LVTIQKADPKTMQQGLIARQRLEYNELSNTKKSEKKNSGVTQKFVDNNAPLKNAKNHV
                     TVFINAVRHKEAHEDLPKTKDVKASTAEQHETFNNVNARNGVASQSSVQNVAHEKVIN
                     NSDLMQLLQKARIMQDGEKTNLSLKMYPESLGRLHIQLGLEQGIVNGRFTVESEQARQ
                     QLMQQLDVVRYELEQSGVHVGGFEVHVRDQRQTEIFQHAEAIPLTQNAEYEEQMSRYS
                     YHDGMLDIII"
     gene            2059..3324
                     /locus_tag="PLX22_07985"
     CDS             2059..3324
                     /locus_tag="PLX22_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOT19878.1"
                     /translation="MEAIESLPNTENQEPPGCSASNTDEPPSSAFTNSNVVTILNKQI
                     VPRSINAKARIFRKLYYPAVNDSQWNDWRWQIANRIRTYEHLSSFIELSPDEIMALTA
                     KQCQLPMAITPYYASLIDRNNYGDPIRRCVIPTINELHHSTGESEDPLAEDNDTPVQG
                     LVHRYPDRVLFLATDFCSTYCRYCTRSRIVGRSNCNLSTSHWEGAIRYIAQHSEVRDV
                     LISGGDPLTMNDSMIEYLLSRLKAIPHVEIIRIGTKVPAVLPQRITHTLVSMLRKYHP
                     LWINIHFTHPDELTPETMKACNRLANAGIPLGSQTVLLKGINDTVETLKKLYHLLLQC
                     RVRPYYLYQCDPIVGSAHFRTSVETGIAMIQGLRGHTTGLAIPHYVIDAPGGGGKIPL
                     LPDYFQGIDGNTTVLKNYEHKYFYYPNTQ"
     gene            3336..4349
                     /locus_tag="PLX22_07990"
     CDS             3336..4349
                     /locus_tag="PLX22_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT19879.1"
                     /translation="MRVGLTYDLRDDYRASGYDEEAIAEFDSIETINAIDTTLGNLGF
                     FTDRIGNVKHLIKRLADGQRWDIVFNIAEGMFGISREAQIPALLDAYQIPYTFSDPLV
                     LSLTLNKAMTKQIISYNGIPTPASMLITNINDCNRCTLTYPLFAKPYAEGTGKGITAS
                     SVIHDRLELTNTCFHLLKKFNQPVLVEEYLPGREFTAGILGNGFNARSAGIIEIILLD
                     NAEKNVYSFANKEQCEQLVEYRFAEDAIAKKAVDVALRAWQVLGCLDAGRVDLKCDSN
                     GNPQFLEVNPLAGLHPSHSDLPIICTKAGISYTRLIANIMYNALTRYNLLDSAPEKLY
                     SSI"
     gene            4474..5460
                     /locus_tag="PLX22_07995"
     CDS             4474..5460
                     /locus_tag="PLX22_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT19880.1"
                     /translation="MEIVFVHQFIHENAPVDEKDVLEQRDELAEIFINMGYAISSRSI
                     QSQLDLILLQELANKDCIVFNLCESLFGRDDLLFLVPFMLEHYNIPFTGSGSMALALS
                     CHKLESKSLMINNFIPTPKYITSSTPYSNNINGTYIIKPYNTHASMHITQDSVIRVHS
                     KHDFDLLLDRLKTSHALFAEEYIDGREFNISLMQTDEGVLILPHAEIIFENYPPNMYK
                     IVDYDAKWAHDSFAYEHTIRNFYFPEDDSPLLNEIDRLSLKVWELFSLNGYARIDYRI
                     RGNEIFILEVNANPCISIYSGFMAAVKQYGLSFQDALDIILKHPIKTNKINI"
     gene            5462..5959
                     /locus_tag="PLX22_08000"
     CDS             5462..5959
                     /locus_tag="PLX22_08000"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT19881.1"
                     /translation="MKQINNTIVYRDYVIASDVVVIPAILDATHFFYQEEIEIALELL
                     NEKINCGDSSTYQFIVAECDNTVIGYICYGKIPLTQSSYDVYWVAVHPDFQGNGIGYT
                     LLLRAEETIKKASGKNLFIETSGRKLYQPTHSFYRKSGYTISAVFTDFYADGDDKLVF
                     YKKLI"
     gene            6037..6270
                     /locus_tag="PLX22_08005"
     CDS             6037..6270
                     /locus_tag="PLX22_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOT19882.1"
                     /translation="MECTCGGMLTEGRSSYSMSGEYYYIVIEDIPAYKCSRCGKILFQ
                     DDVVDKIKALLKRVDRDTKEIITGTPSVHTYDY"
     gene            6300..7739
                     /gene="gltX"
                     /locus_tag="PLX22_08010"
     CDS             6300..7739
                     /gene="gltX"
                     /locus_tag="PLX22_08010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT19883.1"
                     /translation="MMRVRFAPSPTGFLHVGNARTAVLNYLVKKKNNAAYILRIEDTD
                     TQRSTMESEQSILNDLKWLGIQWDEGPDCGGKFGPYRQSERFDIYRQFTEKLLITGNA
                     YHCYCTQEELDAMRAKAAEHNKPFVYPGTCRHLSLEQKLAYEHQGRKPTVRFRVKDGI
                     SIVVNDHIKGKVVFSSENIGGDFIIVRSDGIPVYNYIVIIDDTLMNITHVIRGEDHLP
                     NTPKQMLIAMALDLPAPEYAHMPLLLGPDRTKLSKRHGITSVSIYREQGYLPQALFNY
                     LALLGWTTQSGEEIVPIDELVEQFELSKIGKSPAIFDFQKLKWMNSEYLKNLPSEQVY
                     SLFYPYLEKAGYTTHDREWLNSVIDTIRGNCQIASDIVSEIAIFFGDTVEPDHEAGEL
                     LLQQESKAVIITAYELITSNEVNEHNYHQLLNAIKQKTGLKGKMLFMPFRAMITGRLH
                     GPELDKIIPLLGYQRFKKRIENCYQRFIN"
     gene            8146..>8437
                     /locus_tag="PLX22_08015"
     CDS             8146..>8437
                     /locus_tag="PLX22_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19884.1"
                     /translation="MKKLSIIIVAVVFSICGCDTDSPSEIPVGTWEYDLLVNGVQLGS
                     ATISNKVEDNHYVYSFDLTMGNGQTQSNDVIVETLDCKPVKLEKHTYINAPAQ"
BASE COUNT         2569 a         1586 c         1558 g         2724 t
ORIGIN      
        1 ggttccggca ttgtcattct ccttaaatta ttatatccag cataccatca tgataactat
       61 atcggctcat ctgttcttca tattcagcat tttgtgttaa aggaatagct tcagcatgtt
      121 gaaaaatttc ggtttgtctc tgatccctca catgtacctc aaaaccacca acatggacac
      181 cactttgctc cagttcatac cgtacaacgt caagctgctg catcagttgt tgtcgcgcct
      241 gctcgctctc aacggtaaaa cgcccgttta caattccctg ctccaatcct aactgaatat
      301 gtaatcgtcc taaactttca ggatacattt tcaatgacag attggtcttc tcaccatcct
      361 gcattatcct ggctttttgt aaaagctgca tcaaatcaga attgtttatg actttttcat
      421 gcgctacgtt ttgcactgag ctttgggaag caaccccatt tcgtgcgttt acattattaa
      481 atgtttcatg ttgttctgca gttgatgctt ttacatcctt tgtttttggc aaatcttcat
      541 gtgcttcttt atggcgtaca gcattgataa aaactgtaac atgattttta gcatttttta
      601 aaggtgcatt attatctaca aatttttgtg taacaccaga gttttttttc tcagattttt
      661 tagtgtttga tagttcatta tattctaatc gctgccttgc aatgagccct tgttgcatgg
      721 ttttaggatc agctttttga atcgttacga gattcgcaat gagctttttt atatcacctc
      781 ttttactatt gtttaccatt tcatcattaa ttattttctg catattctca atcaccttat
      841 gcagtgattg cgaattacct gttttagtac ctgatagagt atttgtgcta aaaaggtcat
      901 tggtactggt attgttacgt tgtgcagatg aggtatccaa tccatggagt aatcttcttg
      961 gtgatatatt catatccctg taaggtatcc ttacaggatt atccttattt tttgtgagtg
     1021 tcgccctgct atttatcgta ttgctatttg aaattgttaa aaattgggaa agtgcttcat
     1081 tgtgcaattg tagcttttcc ttatcagcct tttctttttc caaacgtcta tcattaaaac
     1141 tggatacatg tttggcagct ttctcagtgc ttaattcatt tttatttgcc atatcagcaa
     1201 aatatttatc ctggttgtca ttttttgtat ggttagattc ttcaaattca gtaatgacat
     1261 ttttgctgtt gacagtttct tctacctctt ttttttgtaa aatcccatct aaaaatgata
     1321 taaacgattg ctcaggctca gactgccgtg cactattctg attgtgtgtt acaatgtcaa
     1381 aatccaatat aacaggtttt accatatatc aatcctgtaa aatatatatg tcgtatcatt
     1441 aatatcgaat gtgttatatt ttttaatgaa ttaattttaa tttttttaga agcttctccc
     1501 ctttggcggg taatgataac aaataaatat ataaaaattt ttataaatat attaaaaaat
     1561 attgttgcct atattgatat gtgctataaa ttatactgta acttctgcgc tattacctgc
     1621 aatgtttaaa tgcaggcaag tagcagtgta atttggaatg caatacttcc ataattgccg
     1681 atcctccatt ttttacggat cccagagtta agtccttaca atgggagacc tcttggacgc
     1741 tcatccctgt gaaagcaggg atgctttttt tatctttgcc tttatttatt ataataccca
     1801 tcagaagctg taaaaaatat taattttttt tggtaaattt tgtttttttc tttacaagct
     1861 atatatttgt tctttattaa gttttatttt tgtaacaata cgccgatgga aataatatga
     1921 atttttttat agaaaatatt ttttattttt ttgtgacata atacggtatt ttacttgaca
     1981 aattactttt tttataatgg gaagaactaa gtgaattttt atacatataa tcacatgaat
     2041 aagtgaggga acatataaat ggaagcaatt gaatcactgc cgaataccga aaatcaggag
     2101 cctccgggtt gttccgcctc aaatactgat gagcctccat cgtcagcttt taccaattca
     2161 aacgttgtaa ccatcttaaa taagcaaatt gttcccaggt caatcaatgc aaaggcgcga
     2221 atatttagaa aactgtatta tcctgctgta aatgactctc agtggaatga ttggcgctgg
     2281 cagatagcta atagaattcg cacctatgaa catttatcaa gttttataga actatcgcca
     2341 gatgaaatta tggcattgac agccaagcaa tgtcagttgc caatggcaat aacaccgtat
     2401 tatgcaagcc ttatcgatag gaataattat ggcgacccca tacgccgttg tgttatacct
     2461 acaatcaatg aacttcacca tagtacagga gaatcagagg accctcttgc agaagataat
     2521 gatacccccg tccaaggatt ggtgcatcgg taccccgata gagtattgtt tttagctact
     2581 gacttttgtt caacatattg ccgttattgt acccgttcac ggattgttgg gcgttcaaac
     2641 tgcaatcttt ccacttcgca ttgggaaggt gcaatcagat atattgcaca acattcagag
     2701 gtacgcgatg ttttaatctc tgggggcgac cctttgacaa tgaacgactc aatgattgaa
     2761 tacctcctat cacgtcttaa agccataccc catgttgaaa ttattagaat tggcaccaag
     2821 gttcctgctg tgttgcctca acgtattacc catacgctgg taagcatgct acggaaatac
     2881 catcccttat ggataaatat tcactttacc catcctgatg aacttacacc agaaacaatg
     2941 aaagcctgca acaggcttgc aaatgccggt atacctttag gcagtcaaac agtactcctg
     3001 aaaggtatca acgatactgt tgaaacactc aaaaaacttt accatctgct gttgcaatgt
     3061 cgtgtccgcc cttattatct gtatcaatgc gatcctatag ttgggtcagc ccacttccgt
     3121 acatctgttg aaacgggaat cgcaatgatt caaggattac gcgggcatac aacaggattg
     3181 gctatcccgc attatgtaat tgatgctcct ggcggcggtg ggaaaatacc ccttttacct
     3241 gactactttc agggtatcga tggaaatacc actgttttaa aaaactatga acacaaatat
     3301 ttctattatc ctaatactca atgactgata gtggcatgcg tgttgggcta acctacgatc
     3361 ttcgtgatga ctacagagca tcaggatatg atgaagaagc tattgctgag tttgatagca
     3421 tagaaacaat caatgcaatc gatacaactc ttggaaatct gggctttttt actgatagga
     3481 taggcaatgt caaacatctt ataaagcgat tagctgatgg acagcgctgg gatatagtat
     3541 tcaacatagc tgaagggatg tttggcatta gcagagaagc tcagatacct gctcttctgg
     3601 atgcctatca gatcccatac accttttctg atccgcttgt tctatcgctt accctgaaca
     3661 aagccatgac aaaacagata atcagctaca atggcatacc aacaccagca tctatgctca
     3721 ttacaaatat taatgattgc aacagatgca cacttaccta tccgttattt gcaaaaccgt
     3781 atgctgaagg gacagggaaa gggattactg catcttcagt gattcatgat aggctggaat
     3841 taacaaatac ctgctttcat cttctaaaaa aatttaatca gcctgttctg gtagaagaat
     3901 accttccggg gagggaattt accgcgggga tattggggaa tggttttaat gcccgatctg
     3961 caggaataat tgaaataatt ttattggata atgccgaaaa aaacgtctat tcgtttgcca
     4021 ataaagagca atgcgagcaa ctggtagagt acagatttgc agaagatgct attgcaaaaa
     4081 aggctgtaga tgtggcattg cgcgcatggc aggttttagg ttgtttagat gccggacgtg
     4141 ttgatttaaa atgcgacagc aacgggaacc cccaattttt agaagtaaat ccattagcag
     4201 ggcttcaccc atcacattct gatctgccca ttatatgcac aaaagctggc atatcatata
     4261 ccaggctaat tgcaaatatt atgtacaatg ctcttacccg ttataatctt ctggatagtg
     4321 cacctgaaaa attatattca tctatataaa aataatggtt gcacaatttt cattacctaa
     4381 aaaatttatt gtatgctgtt atgcttttta tatttattca atcgtagtat cagatcaatc
     4441 cttatataat attatactaa ggcagtgggt tacatggaga tagtttttgt tcatcaattt
     4501 attcatgaaa atgctccggt agatgaaaag gatgtccttg agcagcgcga cgaattagca
     4561 gaaattttta tcaatatggg atatgccata agctcgcgca gcatacaatc acagttagat
     4621 cttatcttgc ttcaggaact ggcaaacaaa gattgcattg tatttaatct ttgcgaaagc
     4681 ctatttggaa gggatgatct tttgttcctt gtacctttta tgttagaaca ttacaacatt
     4741 ccctttactg gttcaggttc aatggcattg gcgctcagct gtcataaact tgaaagtaaa
     4801 tcattgatga tcaataattt cataccaacg cctaagtata taacatcatc aacaccatac
     4861 agtaataaca ttaatggaac gtatatcatt aagccctata atacccatgc atcaatgcat
     4921 attacgcagg attcagttat acgtgtacac tcaaaacatg attttgattt gcttcttgac
     4981 aggctcaaga catcgcacgc tctttttgct gaagaatata ttgatggaag agaatttaat
     5041 atttcactca tgcaaaccga tgaaggagtt ttgatacttc ctcatgctga aattattttt
     5101 gaaaactatc ctccgaacat gtataagatt gttgattatg atgcgaagtg ggcacatgat
     5161 tcttttgctt atgaacatac tatccgcaat ttttattttc ctgaagatga tagcccttta
     5221 ctaaatgaga ttgataggct atccttaaaa gtatgggaat tattttcatt aaatggatat
     5281 gcacgcatag attacagaat cagaggaaat gaaatattta tattagaagt aaatgcaaat
     5341 ccctgtatat caatctacag cggctttatg gctgctgtaa agcaatatgg tttatcattt
     5401 caggatgcgc ttgatataat actaaaacac cctattaaaa ctaataaaat taacatttaa
     5461 catgaaacaa atcaacaata caattgttta tcgtgattat gtcattgcat cagatgttgt
     5521 tgtaataccg gcaattcttg atgctactca ctttttttat caggaagaaa ttgaaattgc
     5581 tttagagcta ttgaacgaga agatcaattg tggcgatagt tccacgtatc agtttatagt
     5641 tgccgaatgt gacaacaccg ttataggata tatctgctat ggcaaaatcc cgcttactca
     5701 atcttcatac gatgtttact gggtagctgt ccatcctgat tttcagggga atggcattgg
     5761 ttatacactt ttattacgtg ctgaagaaac aataaaaaaa gcatcgggca aaaacctatt
     5821 tatagagacc tccggaagaa aactttacca accaacgcat agtttttatc gcaaatcagg
     5881 ctatactatc tctgcagtat ttaccgactt ttacgccgat ggcgatgata agcttgtctt
     5941 ctacaaaaaa ttaatataat tttctttgtt tttttattgt aattagtatc ttgacatata
     6001 tacctttttt taattttctg tatatacgag gttagcatgg aatgcacgtg tggtggtatg
     6061 ctcacagaag gtagaagttc atatagcatg agcggcgagt attattatat cgttattgag
     6121 gatattccag cgtataaatg ttcacgatgt ggtaaaatat tatttcagga tgatgttgtt
     6181 gataaaataa aggcattatt aaaacgcgtt gatcgtgata caaaagagat tatcaccggc
     6241 accccttcgg tgcataccta tgattattaa ggtgtatact atagtaacag gagaacataa
     6301 tgatgcgcgt acgatttgct ccaagtccaa ccggattttt acatgtaggg aatgcacgaa
     6361 ctgctgttct taattatctt gttaagaaaa aaaacaacgc tgcctatata ctacgtattg
     6421 aagatactga cacacagcgg agcaccatgg aatctgaaca atccattctc aatgatttaa
     6481 aatggctggg catacaatgg gacgaaggcc ccgactgcgg tggcaaattt ggcccttatc
     6541 gtcaatccga gcgatttgat atttaccgcc agtttaccga aaaattgctt ataaccggta
     6601 atgcctatca ttgctactgt actcaggaag agcttgatgc catgcgcgca aaggctgcag
     6661 aacacaataa accatttgta tatccgggaa cctgccgcca tttatcatta gagcaaaagc
     6721 ttgcctatga acatcagggc agaaagccaa cagttcgctt cagggttaaa gacggtataa
     6781 gcatagttgt taacgatcat ataaaaggta aggttgtctt cagcagtgaa aatataggag
     6841 gcgattttat tattgtgcgt tcagatggta tcccggtata taattacatt gttatcattg
     6901 acgatacact gatgaatata acccacgtca tccgtggcga ggaccatctg cccaatacac
     6961 caaagcagat gcttattgcc atggctcttg atcttccagc ccctgaatat gctcatatgc
     7021 cccttttgct tggtcctgac cgcacgaaat tatcaaagcg gcatggcata acctccgttt
     7081 caatataccg tgagcagggg tatctgcccc aggccctttt taactatctt gcactgctgg
     7141 ggtggacaac ccaatcaggt gaggagatag tccctataga tgagctcgtg gaacaatttg
     7201 aattatcaaa aattggtaaa agcccggcta tctttgattt ccaaaaactc aagtggatga
     7261 attctgaata tcttaaaaac cttccctcag agcaggttta ttctttattt tacccttacc
     7321 ttgaaaaagc aggatataca acccatgaca gagaatggct caacagcgtt attgacacca
     7381 tcagagggaa ttgccagata gcttcggata tagtcagtga aatagcaata ttctttggtg
     7441 atacagttga acctgaccat gaagcaggcg agcttttatt acagcaggaa tcaaaagctg
     7501 ttattataac tgcctatgaa cttatcacca gtaatgaagt taatgaacat aactatcacc
     7561 aattgctgaa tgctataaaa caaaaaactg gattaaaagg caagatgttg tttatgccgt
     7621 tcagggcaat gattaccggc aggcttcatg ggcctgaact ggataaaata ataccactat
     7681 taggatatca gcgttttaaa aaacgcattg aaaactgtta tcagcgattt atcaattaaa
     7741 gacttacctt tatggtgtac gatagttata tgaaaacaac aggcgttttt cacattggac
     7801 gttttacatt gatattttat gtgacaaact attataatat cattaatgca gcacaataat
     7861 gtaccaaatt attctatgaa aagttatcca tggacgacat gcggccgtag gggtataaaa
     7921 aagcgttatc gtcatgctaa ggtgaagcct aagaatctcg tcgtttaaag aatagatgct
     7981 tcagtcgcta atgtttcttc agtatgttag gagtgtgaag tgtcatcctg aatcttgtcc
     8041 tgaactggtt tcaggattat ttcaggatct aacgttacat atgtagatta cgggtcaagc
     8101 ctggaatgac agcagtgtat aattcttttt ttggaggagt tgcacatgaa aaaattatct
     8161 atcatcatag ttgctgtagt attttcaatt tgtggatgtg acaccgatag cccaagtgaa
     8221 atacctgttg gtacatggga atatgatctg cttgtaaacg gtgtccagtt aggaagcgca
     8281 acgatttcaa acaaagttga ggataaccat tatgtttaca gctttgattt gaccatgggc
     8341 aacggccaga cacaatcaaa tgacgtaatt gttgaaacgt tagattgcaa acctgtaaaa
     8401 ttagaaaagc atacctatat taatgcaccc gcacaac
//
LOCUS       DAOSOX010000100         8214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8685_length_8214_cov_8.111288, whole genome shotgun sequence.
ACCESSION   DAOSOX010000100 DAOSOX010000000
VERSION     DAOSOX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8685_length_8214_cov_8.111288"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PLX22_08020"
     CDS             <1..1473
                     /locus_tag="PLX22_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19885.1"
                     /translation="QAMRDEISKQNLLALLKQYRLFNLSKLIKKSINKNDLNRIIKSE
                     LGKTIFTFKHFTIDKSHVELAEDYCFHAENYQSVKSSINFKTLKNIISQHADMLLRRL
                     KRFGIISNEFSDYRDAKLDYIFTVLLEDLASLLNESDLAEVKNTHAFRSCLLKVDALL
                     DPVDTYNNDIVSFIHEHKLCTQVDIIDSLPDIDEELIEKWRESNYCKNAKVIAFTNPE
                     DNHRYYCDGTVLISYLSELNQLINYQRDKFEKLGYLEKDKIKKTYAILIKIAENIIAG
                     DAYDELSLNSEQKQILQKIIDDFDKQKIEELPSQPVTIVKQTKQKKSLLKRFVDAFLS
                     LFKRKTIIKPITQTHVFSSPVHQLSKKTKNFYDTISSINKNIIAMTEVIDITDENESD
                     INELIEELRNTGLKIVIPIYNARKVLYPRRSQKLLIPDIEYVLVSPVVMRSYDAIKEF
                     SDSLVGKKLNGEVIPASGIIAIEKYLYTLYRQKRNLMIKK"
     gene            complement(1487..1756)
                     /locus_tag="PLX22_08025"
     CDS             complement(1487..1756)
                     /locus_tag="PLX22_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT19886.1"
                     /translation="MPTYEYVCTSCNNKFELFQSIKDEPVKTCAQCGGEVKRIIGAGA
                     GIIFKGSGFYVTDYKNNSNDKLIKSSSEPSCASCQSKTCEANTES"
     gene            complement(1965..4622)
                     /locus_tag="PLX22_08030"
     CDS             complement(1965..4622)
                     /locus_tag="PLX22_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT19887.1"
                     /translation="MGSIILFNSFSLGTIIPIIFFLTTGFFLYNTKDKSEATTILAKA
                     LIILGVFNFGYFISSSVYHPLAAYHRWLTILTILLGIAYLGYFYFFYPSPKAPRAAKI
                     YIIMGYILSFGALIAFIIVSLQSDIVFLFRGHYYDFDADEISKQIGVLIMVGILCSFI
                     VLWWRFFTSNKEEKKILLLLFLFTVIGTVVPSTTNTLSREGVIDRDVFHTAWTLFNLL
                     GFFLVFIVYINNSKERFSFLGKLIGIILVTVLCLLQIIGFIVLKKNEKDFDEIYYRSA
                     LLTVLNNQTMDNYIYHATYNIANNTWTQNLPDSYNKDDIAMEMKHAYTWNMIFSHAEN
                     NTVEGLQLLLDNTPVSFQGYVATLRYYIEQHKNTIDYSALMAFCRELDAKVYHFHHKI
                     SQLKGPNFRNDLKLLLPKQEPILQQFTSAIANYLETTSKEGEELKHDITQFLIPVQFP
                     GMRTYHVATDGQHVVAYRYINNEIIHEIAFPYTAYRSFMHSTVAILIAIVIIVYVFIR
                     FGIRLFFAGILVKPLRQLTDGLKEVDKGNFDVAIPVQSGDEFGYITSAFNKMVESLKN
                     LFQSTHAKSEEVKRLSSDLNISATKLYDIARELSTIVEETSSAYEEMSSSFESNLNAI
                     QQQANSMDVIKNDIESIDASSSQVSQRITRLSNSIEQAVKQSEEGEVTITKSINAIES
                     IAEYLKEVEQTIVNINEIADKINLLALNAAIEAARAGESGKGFSVVADEVNKLADQTT
                     DIVKGIHATIADQAKMITNEIQYISKATEAIKAIRKGIGETFNVLKDTVDFTNELGIK
                     NQDIRSKLEKFKELSGSVYNFSMEQKVTIDELTKAVNAIIEISQKALESAEFVQGFSK
                     ILDLSAQELSESIGYTANSNSNSESDNNT"
     gene            4881..4953
                     /locus_tag="PLX22_08035"
     tRNA            4881..4953
                     /locus_tag="PLX22_08035"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4914..4916,aa:Thr,seq:cgt)
     gene            complement(4963..6273)
                     /locus_tag="PLX22_08040"
     CDS             complement(4963..6273)
                     /locus_tag="PLX22_08040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT19888.1"
                     /translation="MINQRQKTVVIIGAGFGGLWVAKELSNTNYNVILIDKNNYHTFL
                     PLLYQVSAAEIEPEQIASPVRFIIRNTKNIRFIRGEVTAIHYANKTIECLGQHIPFDY
                     LVVAAGSVNSYFNISGASQYSFPLKTLDDAMILRNHVLSCFERASFIDDKNQRKRLLT
                     IVIVGGGPTGVEFAGALAELVHGPLHKDFPELDYEEISVCLIEAGDRILSMYPPILSE
                     YAKSELIKKGVNVLTHANVSKIEATGVYLNDSPIIPTETVVWTAGVKGEMIKSDCNLK
                     ISTNGRIQVNDNLNIPGYDDVFIIGDLAQYSQDGKTLPMIAPVAIQQGKYVGRLLQLI
                     AKNKKAKPFHYKDKGSMVTIGRNHAVTQVAGLHLKGYIAWIIWIFIHILYLIGFRNKL
                     FVMINWVWDYIFFEKSVRLILPRCCDNPDNTTCKRRFPNNHFCN"
     gene            7055..7918
                     /locus_tag="PLX22_08045"
     CDS             7055..7918
                     /locus_tag="PLX22_08045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19889.1"
                     /translation="MNNSLYDSNNSLLKPADVFANNDEYIAYIQTLTIPGVKFTIQTI
                     EKGDNFWKIAKEHKIDIDTLIGANPLWESITAKVDQKIVIPSQKGVLHFVADINEINS
                     LHSLYDVSEKNVLTPSLFLLRLQSIVSKPEKPIAVFVVDARPKADVMTPQLSKQFTIR
                     EMFRSPLGGRLTSFYGGRLHPIFRQWGFHNGLDIAAPYGTPVGAARGGVVISCGWMGG
                     YGKAVIIQHDEGFKTLYGHLSSIHVRPGQHIKAGQFVGRVGSTGWSTGPHLHFTLWQY
                     NKLINPMKVLW"
     gene            7929..>8214
                     /locus_tag="PLX22_08050"
     CDS             7929..>8214
                     /locus_tag="PLX22_08050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19890.1"
                     /translation="MYQKLIKEYFEKASLYYTKEIISRFNQFLHLLLQYNTSHDLTRI
                     TSIDEIIIKHFVDSLFITKFITIPSPLLDIGTGAGFPGIPLAIVYPDKRFI"
BASE COUNT         2762 a         1409 c         1281 g         2762 t
ORIGIN      
        1 caggcaatgc gagatgaaat atcaaaacaa aatttattag cgctgttaaa gcaatatcgt
       61 ctttttaatc tttcaaaact tataaagaaa tccattaata aaaatgactt aaacaggatt
      121 attaaatcag aattgggtaa aacaattttt acattcaagc attttaccat agataaaagt
      181 catgttgagc tggctgaaga ttactgcttc catgctgaaa attatcagag cgttaaaagt
      241 tctattaatt ttaaaacatt gaaaaatata atatctcaac atgctgatat gcttttgcgc
      301 aggcttaaac gatttggtat tatatcaaat gagttcagtg attaccgtga tgccaagctg
      361 gattacatat ttacggtgct tctggaagat ttagcatcgc tacttaatga aagtgattta
      421 gcggaggtta aaaatactca tgctttcaga tcttgtctat taaaagttga cgccctgctt
      481 gatcctgttg atacctataa caatgatatt gtttcattta tacatgaaca caaattatgt
      541 acgcaggtag atattattga tagtttgcca gatatagatg aagaactcat tgaaaaatgg
      601 cgtgaatcaa attattgtaa aaatgcaaag gtaattgctt ttactaatcc tgaagataat
      661 catcgctatt attgtgatgg aacggtattg attagttatc tgtcagagct taatcaatta
      721 atcaattatc agagggataa atttgaaaag ttaggttatc tggaaaaaga taaaataaaa
      781 aaaacatatg ctattttaat taaaattgct gaaaatatta ttgcagggga tgcatatgac
      841 gaactatcac ttaatagcga acaaaaacaa attctacaaa aaattataga tgactttgat
      901 aaacaaaaga ttgaagagtt gccatcgcag ccggttacca ttgtaaaaca aacaaagcaa
      961 aaaaaatcat tattaaaacg atttgttgat gcatttcttt ctctatttaa aagaaagaca
     1021 ataataaagc ccattacaca aactcatgta ttttcatctc ctgtgcatca attaagtaaa
     1081 aaaacaaaaa atttttatga tactatcagc tcaattaata aaaatattat agccatgaca
     1141 gaggtcattg atattactga tgaaaatgag tcagatataa atgaattaat tgaagaatta
     1201 agaaatacag gattaaaaat agtaatcccc atttataatg cacggaaggt gttatatcca
     1261 cgaagaagcc agaaattatt aatcccggat attgaatatg ttcttgtatc tcccgttgtt
     1321 atgcgatcgt atgatgcaat aaaagaattt agcgattcac ttgtaggaaa aaaactcaat
     1381 ggtgaagtta ttcctgcttc tggtattatt gcgatagaga agtatctgta tacactctat
     1441 cgccagaaaa gaaatctgat gataaaaaaa tagttattct attattctac gattctgtat
     1501 tagcttcaca tgttttggat tggcatgaag cacatgaagg ctctgaagaa gatttaatta
     1561 acttatcatt agaattattc ttatagtcgg ttacataaaa gccggatcct ttaaaaataa
     1621 ttccggctcc agcacctata atacgtttaa cttcacctcc gcattgagca catgttttta
     1681 ctggttcatc ctttatagac tgaaacaatt caaatttatt attacaagag gtacaaacat
     1741 attcgtaggt aggcatttca tccctccata ttacgcataa atagtataat tttcatgaat
     1801 ataattaata tatttaattg aatgattaat actataaata atgtatacaa atatagcaac
     1861 aaatcaataa tttttcttta aaaaatttta aagtaacaaa tttataaaca taaaagatgt
     1921 tgtactttat actacaatct aaatactaca atccgatata caaattacgt attattgtca
     1981 gattctgaat tagaattaga attagcagta tatcctatac tttcacttaa ctcttgtgca
     2041 cttaaatcaa ggatttttga aaacccctgt acaaattcag cactttcaag cgctttctga
     2101 cttatttcga taattgcatt gaccgctttg gttaactcat caatggtaac tttttgttcc
     2161 atcgaaaaat tatacactga cccggatagt tctttaaatt tttcaagttt tgatcgtata
     2221 tcttgatttt ttatccctaa ttcgttggta aaatcaacgg tgtctttcag tacattaaag
     2281 gtttcaccaa tgccttttct aatagcttta atagcttcag ttgctttact tatatactgt
     2341 atctcattgg tgatcatttt agcctgatcg gcaatggttg catgaatccc ctttactatg
     2401 tcagtggttt gatctgctaa tttatttact tcatcagcaa caactgaaaa tcctttaccg
     2461 gattctcctg ctcgtgcagc ttctatggca gcatttaatg ccagcaggtt aattttatca
     2521 gctatttcat tgatattcac aatggtctgt tcaacttctt ttaaatattc agctattgat
     2581 tcaatagcat tgattgattt ggtaatggta acttctccct cctcagattg ttttacagct
     2641 tgctcaatag aatttgataa acgcgttatt cgctgtgaca cctgactgct gctggcatct
     2701 attgattcaa tatcattttt aatcacgtcc atactgttag cttgctgttg aattgcgttg
     2761 agattggatt caaacgacga tgacatctct tcgtatgcgc tggaggtttc ttctacaatg
     2821 gttgatagtt cccttgcaat atcatataat ttagttgctg aaatattaag atcagagctc
     2881 aatcgtttta cttcttctga ttttgcatgt gttgattgga acaagttctt taacgattca
     2941 accatcttat taaaagcgct ggtaatataa ccaaattcat cgccggactg taccggtatg
     3001 gcaacgtcaa aattgccttt atcaacttct ttcaaaccat cagttaactg acgaagaggt
     3061 ttaacaagga tgcctgcaaa aaataatcgt atcccaaagc ggataaatac atatactata
     3121 ataacaattg ctattaatat ggcgacagtt gaatgcataa atgaacgata tgcagtataa
     3181 gggaaagcta tctcatgaat aatttcattg tttatgtatc gataagcaac aacgtgctga
     3241 ccatcagttg caacatggta tgttcgcatt ccgggaaatt gtaccgggat taagaactgt
     3301 gttatgtcat gttttaactc ttcaccttct tttgaagttg tttcaaggta attggcgatg
     3361 gcactggtaa attgttgcaa tataggttct tgttttggta ataataactt caggtcattc
     3421 ctgaagttag ggccttttaa ttgcgatatc ttatggtgga aatgatagac cttcgcatca
     3481 agttccctgc aaaatgccat taatgcacta tagtcaatgg tgtttttatg ctgctcaata
     3541 taataccgta atgttgccac atatccctga aacgatacag gggtattatc caataataat
     3601 tgcaacccct ctacagtatt attttcagca tgtgaaaaaa tcatattcca tgtatatgca
     3661 tgtttcatct ccattgcaat gtcatcttta ttataactat ctggcagatt ttgtgtccat
     3721 gtattattgg caatattgta tgtagcgtga tagatatagt tatccatagt ttggttgttg
     3781 agtactgtta acaaagcaga acggtaataa atttcatcaa aatccttttc attctttttt
     3841 agaacaataa atcctataat ctgcagtaag cacaaaacag ttaccagtat aatcccgatg
     3901 agtttaccta aaaatgaaaa tcgttctttt gaattattga tataaactat aaagacaaga
     3961 aagaaaccta aaaggttaaa caatgtccat gccgtatgga atacgtctct atcaataaca
     4021 ccttccctgc tcagcgtatt ggtggtggaa ggaacgacgg tacctatcac tgtgaataaa
     4081 aacaaaagta ataagatctt tttttcttct ttattgctgg taaaaaaacg ccaccacagt
     4141 acaataaaag aacataaaat tccaaccatt attaagacac ctatctgctt gcttatctca
     4201 tcagcatcaa aatcataata gtgccctctg aataaaaata caatatccga ctgcaacgag
     4261 acaatgataa aagcaatcag tgcaccaaat gaaagtatat aacccatgat aatatatatt
     4321 ttagcagcac ggggagcttt tggagatgga taaaagaaat aaaagtatcc cagatatgct
     4381 atccccagca atatggttaa aatggttagc catcgatgat atgctgccaa tggatgataa
     4441 acacttgatg atataaaata tccaaaatta aaaacaccca gtataattaa agctttagct
     4501 aatatggtgg tagcttctga tttatctttt gtattgtata aaaaaaatcc tgttgttaag
     4561 aaaaaaatta taggtataat tgtgcccaat gaaaacgaat tgaacagtat aatagaaccc
     4621 ataactgcca taccccttat atatattttt ttttagtaac tttgtttaca tataataaat
     4681 cggttaaaat aatcaagaat attttataaa aatataatct gcatagacta cattttattt
     4741 ttaaatgatg ccttgttgaa tctaattcta tttataataa tgattattgg atttaattat
     4801 gatttgcagg ttatggcata tttttcgttt gacaaaaaat tagtgctatt agcttacata
     4861 taaaccatgt gcagcattgt gccagcgtag ctcagttggt agagcggcgc actcgtaatg
     4921 cgcaggtcac cagttcgatc ctggtcgctg gctcattgat tattaattgc aaaaatgatt
     4981 gttgggaaac cttcgtttac atgtagtgtt atcaggatta tcgcagcatc gaggtaagat
     5041 aagccttaca ctcttttcaa agaaaatata atcccatacc cagttgatca taacaaaaag
     5101 tttatttcta aatccaatta aatagagtat atggataaat atccagataa tccatgctat
     5161 ataccctttt aaatgcaaac cagctacctg tgttactgca tggttgcgtc caatggtaac
     5221 catactgcct ttatctttat agtgaaatgg ttttgctttt ttatttttag ctattaattg
     5281 tagaagtcgt ccaacatatt taccctgctg aatggctacc ggtgcaatca taggcaatgt
     5341 ctttccatcc tgactatatt gtgctaaatc tccaataata aaaacatcat catatcctgg
     5401 aatgtttaga ttgtcgttaa cttgtattct cccgttggta gatattttca ggttacaatc
     5461 actttttatc atttcaccct ttacccctgc tgtccacaca actgtttctg tagggattat
     5521 tgggctatcg ttcaggtaaa cgccagtagc ctctatttta ctaacattag catgggtcaa
     5581 aacgttaaca ccttttttta ttaattcaga ctttgcatac tcagataaga ttggaggata
     5641 catgcttaat atcctgtcgc ctgcttctat caaacaaaca ctgatttctt cataatctaa
     5701 ctccggaaaa tctttatgta acggcccatg aaccagctcc gccaatgcac cggcaaactc
     5761 gacccccgta ggacctccgc caacaatgac aatggtaagc aatcttttcc gctgattctt
     5821 atcatcaata aatgaagctc tttcaaaaca ggataaaacg tgattcctga gtatcattgc
     5881 atcatccagt gtttttaatg gaaaggaata ctgtgaagct ccactaatat tgaaatagct
     5941 attgacgctt cctgcagcta ccaccagata atcaaatggt atatgttgtc caaggcattc
     6001 tattgtttta tttgcatagt gtatagctgt tacttctcca cgtatgaaac gtatattttt
     6061 tgtatttctg atgataaatc gtaccggaga agcaatctgt tcaggttcta tttctgcagc
     6121 actcacctga tacagtaacg gtaaaaaagt atggtaattg tttttatcaa taagaattac
     6181 attataattg gtgttcgata gttcctttgc aacccataat ccgccgaaac cagcaccaat
     6241 gataacaact gttttttgcc tttgatttat catggatggc atcctccatt tgatataact
     6301 aataaggatt aataattata tcccttttta tacctcagtc taaaattgtt actgtgatgt
     6361 gcaatctatg aaaatcatat attgcagtat taggcaagat atcatcatca ctcaatgaaa
     6421 atataataaa tgtaactttt tttaactata ccttttcttt ctggtatttt acactgagtt
     6481 catgccaatg ctatatccgt ataagcagtc cctgtaaaca cttctttcac tgttctaaat
     6541 cgtatgaaaa tttctaaaaa tgttttttta aaaaataata aaaattttac caatgcctgc
     6601 gtctacaaag ggaatcaaca atataattat tagagattta cttattgaat aaaaaaattg
     6661 ttgacgtgaa aaacaatcca gctagacttt ccatccatag tattcaatta ttaaataatt
     6721 cattaacacc atttattcga taattatagt attggcatct ttaaaatttt gtttatttat
     6781 tagttacaat atgataaatc ttattctgct tatacctgtt ttcacaaacg atcatgtgca
     6841 atataactat tatagtatag attcattttt atatttgaca ttacatttta caaagttata
     6901 ttaaaaaatt atttttaggt aaatgaataa agcaacaaaa aaaaaattaa taaaaaatta
     6961 aggtattaac atgcttaata ataaactgct attttatagt ataacatttt gtataactct
     7021 attgctcggt tttgcatttt gtataatcaa gttaatgaat aactcattat atgatagtaa
     7081 taatagcctg cttaaaccgg ctgatgtttt tgccaataat gatgagtata tagcgtatat
     7141 acaaacgctt actatacccg gagtaaagtt taccatacaa accatagaaa agggtgataa
     7201 tttctggaag atagcaaaag aacacaagat tgacattgat accttgatag gagccaatcc
     7261 tttatgggaa tctattacag caaaagttga ccagaagata gttattccat ctcaaaaagg
     7321 ggttctccat tttgttgcag atattaatga aataaattct ctccactcac tctatgatgt
     7381 atctgaaaaa aatgtattaa cgccatccct atttttatta cgtttgcaat ctattgtatc
     7441 aaaaccagaa aaacctattg ccgtatttgt tgtcgatgca cgtcctaaag cagatgttat
     7501 gactccacag ctttcaaagc aatttaccat acgagaaatg tttagatcac ctcttggcgg
     7561 acgattaaca tctttttacg gtggaaggtt acatcccatt ttcaggcagt ggggcttcca
     7621 taatgggctg gatatagcag caccttatgg aactccggtt ggagcagcac ggggaggtgt
     7681 tgtaatcagt tgtggctgga tgggcggcta cggaaaagca gttattatac aacatgatga
     7741 aggttttaaa acattgtatg gacatttgtc atcaatacac gttcgccctg gacagcatat
     7801 taaagcaggg caatttgtag gaagagttgg ttcaaccgga tggtctacag gcccacatct
     7861 acactttaca ttatggcaat ataataaact tatcaatcct atgaaagtgc tatggtaata
     7921 gcattaccat gtatcaaaaa ctaattaagg aatactttga aaaagcatca ctatattata
     7981 ccaaagaaat tataagccgt ttcaatcaat ttttacatct tttattacag tacaatactt
     8041 cacacgatct tacccgcatt accagtattg atgaaattat cattaaacat tttgtcgatt
     8101 cattatttat cacaaaattt atcaccatcc cttcacctct ccttgatatt ggaaccggcg
     8161 caggttttcc cggcattcca ttggcaatag tttatccgga taaacgcttc atat
//
LOCUS       DAOSOX010000101         8205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8700_length_8205_cov_9.872270, whole genome shotgun sequence.
ACCESSION   DAOSOX010000101 DAOSOX010000000
VERSION     DAOSOX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_8205_cov_9.872270"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLX22_08055"
     CDS             complement(<1..623)
                     /locus_tag="PLX22_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT19891.1"
                     /translation="MPVNKTELVSFYSHAIAALASFIGLFILIEAAQSYIVKILSAVY
                     AISVIALFSASSLYHGLKRQDNEESLWRKLDHMAIFIMIAGTYTPVCYLYLPATKAFA
                     ILFFQWLLVASGILFKLYFFNAPRIIYTTIYLIMGWMAIVVIKDILHIMDSTSLWYLF
                     AGGIAFTAGAVFYIMQWPKKIQGYVGFHEFFHFFIIAGALCHYLMVYR"
     gene            808..1269
                     /locus_tag="PLX22_08060"
     CDS             808..1269
                     /locus_tag="PLX22_08060"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOT19892.1"
                     /translation="MDTKFVHDSTITIVQQMTHQDANLAGNVHGGTIMKLIDNTGGIV
                     ASRHSGCLVVTASIDRLDFHSPVYVGDLLRLKASVNFVGTTSMEVGVRVEAENFITGV
                     VRHTASAYLTYVALDEKGKPEKVPALIPETDEEKRRNKEAELRRKKRLLEK"
     gene            1282..1596
                     /gene="cutA"
                     /locus_tag="PLX22_08065"
     CDS             1282..1596
                     /gene="cutA"
                     /locus_tag="PLX22_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOT19893.1"
                     /translation="MDIIIMYCTVPDNDVALAIADHLVNNKLAACVNIVSGLMSVYSW
                     KNEICNDSELLLIIKTKKDLYHEVELSIKALHPYEIPEIIALPVVAVSNDYAEWINNV
                     TK"
     gene            1905..2354
                     /locus_tag="PLX22_08070"
     CDS             1905..2354
                     /locus_tag="PLX22_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19894.1"
                     /translation="MKPVRYFLPIFIFIFAISCVSSPKNCKEGDCKNGIGTSTHDNGT
                     YNGSFNNSIREGMGEYNFTNGDRYRGNYSNNMRNGQGEYYWKNGDSYKGNWVDGKRNG
                     FGIYTWADGTRYEGEWKDDKRHGKGKLILNDGTTFDGSWENDLFIRK"
     gene            2379..2711
                     /locus_tag="PLX22_08075"
     CDS             2379..2711
                     /locus_tag="PLX22_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT19895.1"
                     /translation="MNKKNAALISCLILLIPAFILAQSIVKVKKTTTVPKQGTVTFNH
                     QIHKARGITDCKICHHTGSTAKKCGDNGCHWQTQNKGYNAIHTKCLGCHRTKGGKAPT
                     LCTQCHKR"
     gene            2772..3824
                     /locus_tag="PLX22_08080"
     CDS             2772..3824
                     /locus_tag="PLX22_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19896.1"
                     /translation="MPGLITHQRIFFESVTSLKKLKHPDHSTHLLDAQFSHPLFMSSA
                     LAGLLIPNLFDYLPGKRDIFFGNSIKHFLHSDDGLSVIFAMFNTLLNNNHEPNEWVYY
                     QKGFFYGYISHWVTDAMYHPFVFYWAGFPTYKDNRVSYYRNQHLLFEYTIDQYMAYFY
                     NSEPFDYNLDRMIPQKNTILYKASKDIFTNAIATIKPSLTLTPSIISWLKHDGYDIAL
                     QCVKPVYSLKKTRNAHLIKILRLLEKRTYSDFLVRYPDARRINRHILNLHRDRWFNPG
                     GEVGLHYESVDDLFRIVKEKTVTLWKQFEELLNHKEMISPSQFNAVIEFTNAYTGKKG
                     FSLDGMIHQNPIRLRN"
     gene            complement(3829..4911)
                     /locus_tag="PLX22_08085"
     CDS             complement(3829..4911)
                     /locus_tag="PLX22_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19897.1"
                     /translation="MNTKKYINITILLGMIASGSLQVFGSDVTISNVRPNLMNIRTTN
                     GKFYAYIVLLFNENPHFVNLIQADSIPVRLMQTTGFNIVQYEAYLKTLQDTSLKTLAQ
                     QVLRVYKEPDKISDNELEQLTQKLENRNIILRFSKTKDGGEYKYLLDYCIYGKRKPVT
                     INHPLFNVKVRIFNIQPLIYYDEFSTSNSTFYFDMIYINPDEVYNDYIISKRIMDNKS
                     CENMFFVGAHVNEDIRFCIKKSFSSKETIFDEIWKMFEIHELTHKILNNYYYFFDQVT
                     GEELALSSTIYYNTYLGLAILYSYLDYNTINPHRIAASNYIKFLSLYTANGEYIEKPS
                     LIKNLPIEDIKKIARLHFETTIKNLK"
     gene            5158..6072
                     /locus_tag="PLX22_08090"
     CDS             5158..6072
                     /locus_tag="PLX22_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19898.1"
                     /translation="MKLLAEGTINTFCQAIGHIGYPSYIIKGYTKSIMIDAGINLLAL
                     KYYSDLLSLFGEPIALDYVAITHSHYDHLGAIPYLLKKIPNVSVVGAKRIQDLLSKQT
                     VIDFMTHLSDIQKPLFADIVNNEDVSLSYFDITCPVKEGDFIDLGDISCVVYEVPGHT
                     RDSLAYFFPEIGMLCAGEAVGVPQGINGEIVQVEFLSSYDDYCKSLEKMLSLKPQTLC
                     MAHGFVYTDDDVTKFLELSITSTKQYKELLLQYLDRVNGDIEKAIELITFEEYDKKGL
                     IYQERNAYISNLSAQVKLVAKEHNYIKG"
     gene            complement(6062..6268)
                     /locus_tag="PLX22_08095"
     CDS             complement(6062..6268)
                     /locus_tag="PLX22_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19899.1"
                     /translation="MSAYDKKRKTNKKATLFDKVFTVIIQYKETSLFRWIYNHPGIAM
                     SVVALWGIIIIVMFMWFVISLATP"
     gene            complement(6475..7965)
                     /locus_tag="PLX22_08100"
     CDS             complement(6475..7965)
                     /locus_tag="PLX22_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT19900.1"
                     /translation="MPNQMPENLKNIKDILDLDEELHKVSPEAETVGEKSGVRAKKGD
                     INFQLKPEELESYLNKYIVGQEEAVEIIATKICTHFNRMTLEQELPDDEKIVGNIKSN
                     MLLIGPTGVGKTYIIKLIAKKIGVPFVKADATKFSETGYVGGDVEDLVRELVHEADGD
                     VAKAEYGIIYLDEIDKIASSGNVYGPDVSRAGVQRNLLKLMEEADVDLKTPHDLASQV
                     EAAMEAQRTGKVTRKKINTKNILFIVSGAFSGLEDIINKRLNKQAIGFDKSQIRREDR
                     QSVLKQVKTEDLIEFGFESEFIGRLPVTVVLNDLDEEGLYKILKNKYSTVVLGKKLDF
                     RAYGIDLEFMDEALKMLAAKAYKERTGARGLLSVFEKTLIKFEKTLPSTDIKKLVVNS
                     VVVDNPESVLKNILLSDNIRKFQKEFLLKHGVVMEFDGEALQIIQEKAREAKMSIKQF
                     CDDLFHDYPYGIKLMNLDRFVITKGAVENPQSYIDEYIRQYYSHKK"
BASE COUNT         2744 a         1448 c         1291 g         2722 t
ORIGIN      
        1 cggtaaacca tgagatagtg gcacaaagct cccgctatga taaaaaaatg aaaaaattca
       61 tgaaatccca catacccctg tatcttttta ggccattgca taatataaaa aactgctcct
      121 gctgtgaatg caatgccacc tgcaaatagg taccatagcg atgtgctatc cattatatgc
      181 aaaatatcct taatcacaac aattgccatc cagcccatta tcaaatagat tgtggtataa
      241 ataattcttg gagcattgaa aaagtataat ttaaatagta tacctgatgc taccaacaac
      301 cactggaaaa acagtatggc aaatgctttt gtagcaggaa gatacaggta gcataccggc
      361 gtgtatgttc ccgcaatcat aataaatatt gccatatgat caagtttacg ccatagtgat
      421 tcttcattat cctggcgttt taatccatga taaagcgagc ttgctgaaaa taatgcaata
      481 acagaaatag cgtatactgc tgatagtatc tttacaatat aagactgtgc agcttctata
      541 agtataaata atccaatgaa tgaagccaat gcagctattg catgtgaata aaatgaaaca
      601 agttctgttt tgtttaccgg catagtataa catccccata ttttgtttat atagtactat
      661 tctataaaga cagtattata tattttttgg ttgcaatttt tcatttgctt attcaatatt
      721 tttacatttt aaaatgtata atgaacatct acaataatta taaagatact atatcaagca
      781 taacatcttt catgtaggga taaaggtatg gatacaaaat ttgttcacga tagtaccata
      841 acaatagttc aacagatgac acatcaggat gctaatttag ccggcaatgt ccatggtggc
      901 actataatga agctcattga taataccggt gggattgttg cctcccgaca ttccgggtgt
      961 ctggtagtta ccgcctcaat tgacagattg gattttcata gccctgttta tgttggtgat
     1021 ttactgcgtc ttaaggcaag tgttaacttt gttggtacaa catcaatgga ggtgggcgtt
     1081 cgagttgaag ctgaaaactt tataaccggc gtagtgcggc acactgcctc agcatatctt
     1141 acctatgtag cacttgatga aaaaggaaaa cctgaaaaag ttccagcact cattcctgaa
     1201 actgatgagg aaaaaagacg gaataaagaa gcagaactac gacggaaaaa acggttactt
     1261 gaaaagtaaa aggacaataa tatggatata attataatgt attgtaccgt ccctgacaat
     1321 gatgttgcac ttgctattgc agatcatctg gtcaataaca aattagcagc atgtgttaac
     1381 atagttagcg ggctcatgtc agtgtattca tggaaaaacg agatatgcaa cgattcagaa
     1441 ttattgctta taatcaaaac aaaaaaagat ctttaccatg aagttgaatt aagcattaaa
     1501 gcactgcacc cgtatgaaat ccctgaaatt attgcattgc cggtagttgc agtatccaat
     1561 gattatgcag agtggataaa caatgttacc aaataaaaat tccgtacttg tggtacggaa
     1621 ttttatataa agacgcatgg caaggtcatc accaatgcag tcttaaaata agttatcaca
     1681 aaaatgtata tctttcaaaa aaaatatttt aattaatcta attactatgc atacaatgct
     1741 tataaaaaaa atataaactt tattgctaca tagcagcttt tatcccgata gaactatcgc
     1801 aaagcacaaa aaataaaaaa actaattgct aattatatat tcattgtcta taatgcctta
     1861 attatagaaa gattatcata attcttgagg taaggaggtg tttcatgaaa cctgtaagat
     1921 attttttacc tatttttatt tttatttttg ctataagttg tgtttcgtca ccaaaaaatt
     1981 gtaaagaagg tgactgtaaa aatggaatag gaacatctac tcatgataat ggcacctata
     2041 acggtagctt taataatagt attcgtgaag gtatgggtga atacaatttc acgaacggtg
     2101 accggtatag aggtaattac agcaataata tgcgtaatgg acagggtgaa tattactgga
     2161 agaatggcga tagctacaaa ggcaactggg tagatggcaa acgaaatggt tttggcattt
     2221 atacatgggc tgacggaacc cgctatgaag gtgagtggaa agatgataag cgccatggga
     2281 aaggaaagct aattcttaac gatggtacca cctttgatgg gtcatgggag aatgacctgt
     2341 ttatccgcaa atgatataca ttattatagt gaggtactat gaataaaaaa aatgcggcgt
     2401 tgataagctg ccttattcta ttgatacctg cttttatact tgctcaaagc attgtaaagg
     2461 ttaaaaagac tactactgtc cctaaacagg gtactgttac ctttaatcat caaatccata
     2521 aagccagggg aattacagat tgcaaaatat gtcatcatac tggttctaca gcaaaaaaat
     2581 gcggtgataa cggttgtcac tggcaaacac aaaataaggg ttacaatgct attcatacca
     2641 aatgcctggg ttgtcacaga actaaaggcg gtaaggcacc tacactttgc acacagtgtc
     2701 ataaacggtg ataaaacaaa aaggggggaa tatacaattc ccccttaata aattttaaaa
     2761 tcatattcat tatgcctgga ttaataacgc atcaacgtat cttttttgaa tcagttacat
     2821 cattaaagaa gctaaaacat cctgatcata gtacgcattt gcttgatgca cagttttccc
     2881 atcctctttt catgtcatct gctctggcag gattgctaat accaaatctt tttgattacc
     2941 taccaggtaa gcgcgacatt ttttttggca acagtataaa acattttttg cattcggatg
     3001 atggtctatc tgttattttt gccatgttta acaccctttt aaataataac catgaaccaa
     3061 atgaatgggt atactatcaa aaaggttttt tttacggata tatttcacac tgggttactg
     3121 atgcgatgta tcatcctttt gtattttact gggctggatt cccaacatat aaagataaca
     3181 gggtgtcgta ctatcgcaat caacatcttc tctttgaata tactattgat caatatatgg
     3241 cttatttcta taattcagaa ccttttgatt ataatttaga ccgtatgatc ccacaaaaaa
     3301 acactatact gtacaaagca tccaaagata tttttactaa tgctatcgcc accataaaac
     3361 cgtccctcac tcttacacca tccattattt catggttaaa acatgatggt tatgatatcg
     3421 cattacaatg tgtaaaacca gtttattcat taaaaaaaac aagaaatgcg catcttatta
     3481 agattcttcg cttacttgaa aaaagaactt acagtgattt cctggtacgg tatcctgatg
     3541 cacgaaggat taaccgtcat attttaaatc tgcatcgtga tcgatggttt aatcccggcg
     3601 gtgaagtggg cttacattac gaatcagttg atgacctttt cagaattgtg aaagaaaaaa
     3661 ctgtcacact ctggaaacaa tttgaagaac tattaaacca caaagaaatg atttcacctt
     3721 ctcaattcaa tgctgtaatc gaatttacaa atgcctacac cggaaaaaaa ggtttcagcc
     3781 ttgatggcat gatccatcaa aatcctatac gattacgcaa ttgagatgtt attttaaatt
     3841 ttttattgtg gtttcaaaat gtagcctggc aattttttta atatcctcaa tgggtaaatt
     3901 ttttataagg gatggttttt caatatattc accgttagct gtgtataaag acaaaaattt
     3961 aatataatta gaagctgcga tgcgatgagg attaatagta ttataatcga gatagctata
     4021 tagtattgca aggccaagat aggtattgta ataaatggta ctgcttaatg ccagttcttc
     4081 acctgtcacc tgatcaaaaa agtaataata attattcaaa attttatgtg ttaactcatg
     4141 tatctcaaac attttccata tctcatcaaa gattgtttct ttgctggaaa atgatttttt
     4201 tatgcaaaag cgtatatcct cattaacatg tgcacctaca aaaaacatgt tttcgcatga
     4261 tttgttatcc ataattcttt tagatattat gtaatcattg tacacttcat caggatttat
     4321 ataaatcata tcaaaataaa acgtcgagtt acttgtcgaa aattcatcat aataaattaa
     4381 aggttgtata ttaaaaattc ttacctttac attaaaaagc ggatgattga tagttaccgg
     4441 ttttcttttc ccataaatgc aataatccag taaatattta tactcaccgc catcttttgt
     4501 ttttgaaaat cgcagtatta tatttctgtt ctccagcttt tgtgtcagct gttcaagttc
     4561 attatcagat atcttatcag gttctttgta cacccgcaat acctgttgtg ctaaagtttt
     4621 taaagaagta tcctgtaatg ttttcaggta tgcttcgtat tgcacaatat taaaaccagt
     4681 tgtttgcatc aaccttactg gaatactatc tgcctggata aggtttacaa aatgagggtt
     4741 ttcgttaaac aacaatacaa tatatgcata aaatttgcca ttggttgttc taatattcat
     4801 caggtttgga cggacattag agatagttac atctgaacca aaaacctgta atgatcctga
     4861 agcaatcatc cccagcaaaa ttgtgatgtt aatatatttt ttagtattca taacaatatt
     4921 gtatacattc ttactataaa caatgtagct ttattttcaa aattatgcaa attattaata
     4981 tggtacaaat aattttaaac ataaatattt taccaaatgt tattgaaaaa tttcttacag
     5041 tggaaaagat agacattttg tagcttatta tagctaaact gtatagaaaa tatttttatc
     5101 atcatattgt gcaacaatag ttgtagcaat aatagtatct ataaaaagga taaaaaaatg
     5161 aaacttcttg cagagggaac aattaataca ttttgtcagg caattggcca tattggctac
     5221 ccctcctata ttattaaggg gtatactaaa agtataatga tagatgcagg gattaatctt
     5281 ttagcactaa aatattacag cgatctgcta tcactatttg gtgaaccaat tgccctcgat
     5341 tatgttgcta ttactcactc acactatgac catctgggag ctataccata tttactgaag
     5401 aaaatcccca atgtatcagt ggtaggtgcc aaacgcatac aggatctgct ttctaaacaa
     5461 acagttattg atttcatgac gcatctcagc gatatacaaa aacctctttt tgcagatatt
     5521 gtcaacaatg aagacgtatc gctttcatac tttgatataa cttgccctgt taaagaagga
     5581 gatttcattg atttagggga tattagttgc gttgtatatg aagttcccgg tcatacacgc
     5641 gattcccttg catatttctt ccctgagatt ggaatgcttt gtgctggcga agctgtcggg
     5701 gttccccagg gcattaatgg agaaattgtg caggttgaat ttttatcctc gtatgacgat
     5761 tactgtaaat ctttagaaaa aatgttatcc cttaaaccgc aaaccctttg tatggcacat
     5821 ggttttgtat acaccgatga tgatgtaact aagtttttgg aattatccat tacatctacc
     5881 aaacaatata aagaactttt attacaatat cttgaccggg tgaatggcga tattgaaaaa
     5941 gcaattgaac tgattacttt tgaggaatat gataaaaaag ggctgatcta tcaagaacgg
     6001 aatgcataca tatctaattt gagtgcacag gttaaattag ttgcaaaaga gcataactat
     6061 atcaaggggt agcaagcgaa attacaaacc acataaacat gacaatgatt ataatgcccc
     6121 ataatgcaac cacactcatg gctataccag gatgattgta tatccatctg aaaagagatg
     6181 tctctttata ttgaataata actgtaaaaa ccttatcaaa aagagtggct tttttatttg
     6241 tttttctctt tttatcataa gcagacattg gttcccccgg catttataaa ataacgtaca
     6301 tcatgatatt atatgtaatt taatttaata attgtcaatc aataaaattt tgtatatatt
     6361 tttaatatga gagtttttgc aacttttttt caaagtttat aaccttttga attcaggtta
     6421 tattcctctc tgtgacctga taaagtatta ctaactaaag tttttgctat gcctttattt
     6481 tttatgacta taatattgcc gtatatactc atcaatataa ctttgaggat tttcaacagc
     6541 acctttagtt ataacaaacc tatcaaggtt cataagtttt ataccatagg gataatcatg
     6601 aaataaatca tcacaaaatt gcttgatgct catttttgct tcacgtgctt tttcctgtat
     6661 tatctgtaaa gcctctccgt caaattccat aacaacgcca tgttttaata aaaactcttt
     6721 ctgaaatttt ctaatattat cactcaacaa tatgtttttt aatacacttt caggattatc
     6781 cacaactact gaattaacaa ccaacttttt aatatcagtt gatggcaatg ttttttcaaa
     6841 tttaataagt gttttttcaa atacacttaa taatccccgg gcgcccgttc gttctttata
     6901 tgccttggct gcaagcatct tcaatgcttc atccataaat tccagatcta tgccatatgc
     6961 cctgaaatca agcttcttcc caagaacaac agtactatat ttatttttaa gtatcttata
     7021 taatccctct tcatcaagat cattcagtac taccgttaca ggcaaacgcc caatgaattc
     7081 tgattcaaat ccaaattcaa ttaaatcctc ggtctttacc tgcttcaaaa cgctttgacg
     7141 atcttctctg cgtatctgcg atttatcaaa tcctatagcc tgcttgttta agcgtttgtt
     7201 aatgatatcc tccaaaccgc taaatgcacc agatactatg aataatatat ttttggtatt
     7261 tatcttttta cgtgttactt ttccggtacg ctgagcttcc atggcagctt caacctgcga
     7321 ggctaagtca tgtggtgttt taagatcaac atcggcttct tccataagtt ttaacaaatt
     7381 acgttgaaca cctgctcgtg atacatcagg tccgtagaca tttccacttg aagcaatctt
     7441 atctatttca tccagataaa taatcccata ttctgccttt gcaacatcac catctgcctc
     7501 gtgaaccagc tctctgacca gatcttctac atctccacca acatatcctg tttcactaaa
     7561 cttggttgca tcagctttta caaatggtac acctatcttt ttcgcaatga gcttgataat
     7621 atatgttttt ccaactcctg ttggcccaat taaaagcata ttacttttaa tattgccaac
     7681 aatcttctca tcatcaggta attcctgttc cagcgtcatg cgattaaaat gagtacatat
     7741 tttagtagcg ataatctcta ctgcttcttc ctgccccact atatatttat tcaaataact
     7801 ttctaattcc tcaggtttca gttgaaaatt gatatcacct tttttggctc tcacgcctga
     7861 tttttcacca acagtttcag cttcaggcga tactttatga agctcttcgt ctaaatccaa
     7921 tatatctttt atatttttta aattttctgg catctgattt ggcatagaat atcctcatac
     7981 ccctaaaaaa attgattaat atgcgataca tagtatcaca tattaataat ataccataaa
     8041 attattggtt gtgtgaaaat aaagcacaaa atatttaaca attttagatt tcaggttctt
     8101 ttcgataaag taatatttat aaaaattgaa taataaagct aatttcaaga atgaaaagca
     8161 acaccaaata aaatagtata acttagtaat tattttcaac aacat
//
LOCUS       DAOSOX010000102         8146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8791_length_8146_cov_9.398097, whole genome shotgun sequence.
ACCESSION   DAOSOX010000102 DAOSOX010000000
VERSION     DAOSOX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_8146_cov_9.398097"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLX22_08105"
     CDS             complement(<1..1322)
                     /locus_tag="PLX22_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT19901.1"
                     /translation="MKENLALSFYPVSLEQIQEKVERKQIRQKNKINDLFQLDLFYNS
                     KANNTRIIKDASGNYITRVDGEIPVDLSVRNGERFLFISYDQSILTHGIHKYPAKFFP
                     ELPRWFIKRYSKEGDLILDPFAGSGTTNVEALLNKRHSVGIDIDPFSRLLLKVKVTPL
                     NEKELLFAQQYFLEAIKNFHVDSLSQKDLPDFPYRDNWFNKEILYELAYIKKQIEIMD
                     VSEDIKDYYKISFSSIIRSVSNADDNCTRTVVRKKLNKKVYPADALKKFVEAILVGTP
                     KMIEFSKMVPKHIDVILPENMDARDIKYNDDYFDLAITSPPYANAVDYPRAHQLESYW
                     LGLVEGSLTPLKKKHIGTESVSFNDYSQLHVFNIEIIDLKISAIFKKDPRRAFIVYKY
                     LKDMESNLSEVYRVLKKGGRYIIVVGNNKIRGEVFENWKYIMQLAGKTG"
     gene            complement(1712..1784)
                     /locus_tag="PLX22_08110"
     tRNA            complement(1712..1784)
                     /locus_tag="PLX22_08110"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1748..1750),aa:Glu,seq:ttc)
     gene            complement(1841..1913)
                     /locus_tag="PLX22_08115"
     tRNA            complement(1841..1913)
                     /locus_tag="PLX22_08115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1878..1880),aa:Lys,seq:ttt)
     assembly_gap    1993..2092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2093..2904
                     /locus_tag="PLX22_08120"
     CDS             <2093..2904
                     /locus_tag="PLX22_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19902.1"
                     /translation="SNITENSVKMQWTFANDDSPSELLQYQVIVSQTLTLFSLQEILQ
                     SGLLKEDNWSNQPINQSTITNLSIGKLYHANVCVRDPDKNIAVYTPTSFTTKGRMYLF
                     SAGTYTGDMIFQKASRVVVIVSARDALNALCKSVLQSAYPDLPQDNVIAFISINNDDS
                     IATIPLHFAGIPLDWPVYSPSGIIIAYNWDDLVDSSINKKLQDAGVCDSFWWSGSLAD
                     GTCDALNTCNGWTDGTNSFQGRSGAHNKTEQWLSDNERNCNNQHHLLGLCW"
     gene            2977..4014
                     /locus_tag="PLX22_08125"
     CDS             2977..4014
                     /locus_tag="PLX22_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19903.1"
                     /translation="MLLYMLLPAVIIAQTSFDGHITAGAQAGYSALSGYYKNKLDNGI
                     GAGFFIYAPYNNFIMLNAQLVYYRYTFAESSRSSMQVYALHTIPSLYFSLPHDITIYG
                     GTGLSVQYHTATALKTDAYDSTIKAGWLLNTGIAKSIFKHTIIMLDCQYSLSELSHKK
                     LQVLNIYGKIGYMFALHSAEYYIEQQKEKEKNKAEKITKLYDSGYAYLKNKEHTSALQ
                     CFNQILSLDSGQVEAQQHINKINEAIVHHNHAIALIQQNKKIEALPYLIQASDYIQDA
                     ENILVTTREQLKPEILPLQTKGIEQFTNNDYDGCIATMSIILMIDPDNPTAKAYIVRA
                     KRIKETIKKFQ"
     gene            4016..6721
                     /locus_tag="PLX22_08130"
     CDS             4016..6721
                     /locus_tag="PLX22_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT19904.1"
                     /translation="MRLRYKILIAFLIIIVISTSIPLYIIHNQIQQQKEHLVNEGTIY
                     SMNLAHSIAAIVLLNSGNVAQTSIDALEFVETLKPPFTRELASIQVILAAPNKKYNGL
                     LLVDIPNNKTLTKLPDIIVNKLKSTSTYSYISSNNIRYFEFVAQSPVAMGNIHCIARL
                     LYNEDAVLKPVYRNYTIFVISTVIIIFLAVIFSLSFTRAFSRPIETMIQSIKQFDEGV
                     TNIEIPIKNKTDEIGKLATTIQHLFYMVNLEIRALQQNNRELIRLDKLKDDFLANLSY
                     ELKIPLNGIIHLTNNIIHDYAEKNHPYTTNTLQLITNSAMRLSYLIDDISDFTRLKNN
                     DIVLNKKIVDITGVINFVISLLQPAVASKDITIILSIQSNASPLYVDEQRLQQILLNI
                     IGNSIKYTKQGTITIATQMHDSNTLSIIITSTAGGIPPELVKNIDDFGYDSTVSNKQS
                     GGSALGLAVTKKLIELHDGSIYIESQPDGGSCVTVDFPYDENLISKELATASTNDFVI
                     SLPEDIIQYQPSLMLDTSQGFVYIVDDDPVNVKILQEMLQKANFYIEFSHDPSLLYDS
                     IQKNRIPDIVLLDTILPGTSAFQVCETIRKSYSLHELPIIIMTSKHRTQEIITSFRVG
                     ANDYLSKPYNEEELIARIHNLIMLKRSVKEHNDYVILKHEIRLAHEIHNSVVIQNIPQ
                     LHNITIAHAYIPTRDMGGDFYDVIKIDDDKIGIMIADVTGHGIPAALVCAMLKMAFIN
                     NKEHARYPARMMTLLNKDLSSTIHDNYITSAYSLINTKNKTITVSIAGHPPPLLLHND
                     GTVFKNWTRAFPIGWIGDAKYQETSIHYQHGDKFILYTDGVIELKKGKQIFGEDNVIT
                     CIANSFEKQPAILIDEMIKLLHDWSSIPADEPFKDDVTLLIAELL"
     gene            6789..7166
                     /locus_tag="PLX22_08135"
     CDS             6789..7166
                     /locus_tag="PLX22_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19905.1"
                     /translation="MNCTIIELPGIKIDTINYNRAVCIIKLADVADMDKNDDLLALTM
                     CLINGGLRKIIFDFTSVEFIDSYGIGTIVEIAKYIRQHHNGDVVLINVSERILLIFKP
                     IQFQKFIKIFHSEDEALHYFQYV"
     gene            7193..7579
                     /locus_tag="PLX22_08140"
     CDS             7193..7579
                     /locus_tag="PLX22_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19906.1"
                     /translation="MSFEIELLNLSLINKENVDYDMIIFYMNYPEKIDIDTSREIMLF
                     LTTLIKGGAKKIIVDMKKVTLIDSAGISTLILATKQIRKNNGDLVLSHVNDSIQNILD
                     IIKIEKFIKIFRTDSDAVQYFRYIQT"
     gene            7593..>8146
                     /locus_tag="PLX22_08145"
     CDS             7593..>8146
                     /locus_tag="PLX22_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOT19907.1"
                     /translation="MDISFKDYYTILGVSPAADTGTIKSAFRQKAKETHPDSTKQNSY
                     AQFVLIREAYDILTNPSQRASYDIVWNKYHVKSEPEMTSLFDDFGPPDDRYAQEWEYF
                     VLHPEDYLSRFESTLALIKASLRSIAISVCIPVIVMISILLGVTIAMVTLFVFAIGIT
                     SYSSLIAGIVITLLMVKRFVEISQ"
BASE COUNT         2770 a         1444 c         1301 g         2531 t
ORIGIN      
        1 ccagtctttc ccgctaattg cataatatat ttccaatttt caaaaacttc tccgcgtatt
       61 ttattattac caaccactat aatgtatctt cctccttttt taagtaccct atagacttct
      121 gaaagattac tttccatatc ttttagatat ttatatacaa taaatgctct gcggggatcc
      181 tttttgaaaa ttgctgaaat ttttaaatca ataatttcga tattaaatac atgtaactga
      241 ctatagtcat taaaagatac gctttcggtc ccaatatgtt ttttcttaag aggagtcaaa
      301 gaaccttcga ctaaacctaa ccagtaactt tccaattgat gagctcttgg gtagtcaact
      361 gcatttgcat aaggtggaga ggtaattgct aaatcaaaat aatcatcatt atatttgata
      421 tcgcgtgcat ccatattttc aggtaatata acatctatgt gctttggcac catctttgaa
      481 aattcgatca ttttaggtgt cccgactaat atcgcttcaa caaatttttt caatgcatct
      541 gcaggatata cttttttatt aagctttttt ctaacgactg ttcttgtaca attatcatca
      601 gcattggaaa cggatcggat tattgatgaa aaagaaattt tataataatc tttaatatcc
      661 tcagaaacat ccattatttc aatttgcttt ttaatatatg caagttcata taaaatttct
      721 ttattaaacc aattgtctcg ataaggaaaa tcaggtaaat ctttttgaga taaactatca
      781 acatgaaaat tttttatagc ttctaaaaaa tattgttgag caaataatag ttccttttca
      841 tttaatggtg taactttaac ttttaaaaga agtcttgaga aaggatcaat gtcaattcca
      901 acagaatggc gtttatttaa taatgcctca acattagtag tgccactacc agcaaaagga
      961 tcaaggatta aatccccttc ttttgaatat cgcttaataa accatcttgg caattcaggg
     1021 aaaaattttg cagggtattt atgtatacca tgagttaaaa tactctgatc ataacttata
     1081 aataaaaacc tctcgccatt tctgacagac aaatctacag gtatctcacc gtctacccgt
     1141 gttatataat tgccagatgc atcttttata attctagtgt tatttgcttt actgttatag
     1201 aataaatcga gttggaataa atcattaatt ttattttttt gccttatttg ctttcgctct
     1261 actttttctt gaatttgttc aagagatacc ggataaaagg ataatgcaag attttccttc
     1321 ataaacattc ctttttatac agtagtgtat atttattagt tttgaaatat atctcatatg
     1381 taaagagaac atataaataa ttgcaagtaa taaaataatt cattataaaa ttttatagag
     1441 cacctgtcat aaatgaatac ccggtaagtt aacattaacc atactataac caccatgata
     1501 cgatacaatg aagcgagaat gttatcctga acttgtttca gaatctaatg ttatctactg
     1561 ggattccgaa tcaagcccgg aatgatggtg ggtgttattc tcttttcaaa gaagtaacat
     1621 aatctttata gtatagataa aaaaattcct tgaatttaca aaaataaaaa agggcacttt
     1681 attgacatcg tacccttttt tattttcaat gcatccccga ggggattcga acccctgttg
     1741 ccgggatgaa aacccggtgt cctagacccc tagacgacgg ggacatgtaa taatgagact
     1801 acttaaaaaa ataacattga aactgtcaat attattttat tgagtcgccg gagaatcgaa
     1861 ctccggaccc actgcttaaa aggcagttgc tctaccgact gagctagcga ctcgcaccat
     1921 aattattatt gtaacaaatg gatgaacctt gtataattaa ttgctttttg gtgtcaaata
     1981 aaaaatgtaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatcaaat
     2101 ataacagaaa attctgtaaa aatgcaatgg acattcgcaa atgatgattc tccatctgaa
     2161 ttactccaat atcaggttat tgtaagtcaa acacttactc ttttttcatt gcaagaaata
     2221 cttcagtctg gactgcttaa agaggataat tggtccaatc agcctataaa tcaaagtacc
     2281 atcaccaatc tatccatcgg gaagctatac catgccaatg tctgtgtcag agatcctgac
     2341 aaaaacattg cggtttacac cccaacctca tttaccacaa aaggaagaat gtatctattc
     2401 tctgcgggga catataccgg tgacatgatt tttcaaaaag catcgcgagt tgttgttata
     2461 gtatcagccc gtgatgccct caatgcatta tgcaagagtg ttttgcaatc agcatatccg
     2521 gacctcccgc aggataatgt tatagcgttc atcagcatta ataatgatga ttccattgct
     2581 accatccctc tacattttgc aggtattcct ttagattggc cggtatattc cccatccgga
     2641 atcattatag catataactg ggatgatctt gtcgatagtt ctatcaataa aaagttacag
     2701 gatgctggtg tgtgcgatag tttctggtgg tcaggcagtt tagctgatgg cacgtgtgat
     2761 gcattgaata catgcaatgg atggactgat ggaacaaata gttttcaggg aagaagcggg
     2821 gcacataata aaacagaaca atggctctct gataatgaaa gaaattgtaa taaccagcat
     2881 catcttttag ggttatgctg gtaagcgttt taattatttt taaaactgga acaacgggtg
     2941 atagttattg agtataaata tgttttgagt gtttttttgt tactgtatat gctattgcct
     3001 gctgtgataa tagctcaaac gtcatttgat ggtcatataa ccgctggcgc acaggcagga
     3061 tattctgcat tgtcaggata ttataaaaat aaactggata acggcattgg cgcaggattt
     3121 tttatttatg caccatataa taatttcatt atgcttaacg ctcaactggt atattaccga
     3181 tataccttcg ctgaaagcag cagatcaagc atgcaggtat atgcattgca caccataccg
     3241 tccttatatt tttcattacc gcatgacata accatatatg gaggaactgg attgtcggtt
     3301 caataccata ctgccactgc actaaaaact gatgcgtatg attcaacaat caaagcagga
     3361 tggctactaa acacgggtat tgcaaaatcc atttttaagc atactatcat tatgctggac
     3421 tgccaataca gcttatcaga actatcacac aaaaaattac aggtattaaa catctatgga
     3481 aaaataggtt atatgtttgc actgcattcc gctgaatatt atatagaaca gcaaaaggaa
     3541 aaggaaaaaa ataaagctga aaaaattaca aaactgtatg actcaggata tgcatatcta
     3601 aaaaataagg agcataccag cgctctgcag tgttttaatc agatcctttc tcttgatagc
     3661 gggcaggtag aagcacagca acatataaat aaaattaatg aagcgatagt tcaccataat
     3721 catgccattg cactgattca gcaaaataaa aaaattgaag cccttcctta tttaatacag
     3781 gcatcagatt atatacagga tgctgaaaat attttagtta ctacccgcga acaacttaag
     3841 cccgaaattt tacctcttca aaccaaaggg attgagcagt ttaccaataa tgattatgac
     3901 gggtgtattg caacgatgag tattatctta atgattgatc ctgataatcc aacagctaag
     3961 gcttacattg tacgggcaaa aagaattaaa gaaaccataa aaaagtttca ataatatgag
     4021 attaagatat aaaatactga ttgcgttttt aatcatcata gtgatttcaa cctcaatacc
     4081 attgtacatt attcataatc agattcagca acaaaaagaa catcttgtta atgaaggtac
     4141 catatactcc atgaaccttg cacattctat agctgctatt gtattgctca acagtggaaa
     4201 cgttgctcaa accagcatag atgctttaga atttgtagaa acattaaaac ccccattcac
     4261 cagagaactt gcatccatac aggttatttt agctgcacca aataaaaaat acaacgggct
     4321 gttacttgtt gatattccaa acaacaaaac tttaacaaaa ttacccgata taattgtcaa
     4381 taaattaaaa tcaaccagca cctattcata tatatcatca aataacatac ggtattttga
     4441 attcgtagct cagtctccag tagccatggg taatatccat tgcatagcac gattgctcta
     4501 taatgaagat gctgttttaa aacctgtgta tcgcaattat actatctttg ttatcagtac
     4561 agtcattata atttttttag ctgttatttt ttcattatca ttcacccgtg cattttcccg
     4621 gccaattgaa acaatgatcc agtccataaa acaatttgat gaaggtgtaa ccaatattga
     4681 aatccctata aaaaacaaaa ctgatgaaat aggaaaatta gccacaacca tacaacatct
     4741 tttttatatg gtaaatttag agatccgtgc acttcaacaa aataacaggg aactgatacg
     4801 acttgataag ttgaaagacg attttctggc aaatttatcg tatgaactga agatcccttt
     4861 aaatggcata atacacttaa ccaataatat tatccatgat tatgctgaaa aaaatcaccc
     4921 atatacaact aatacccttc agcttatcac caacagcgcc atgcgattat cctatctgat
     4981 tgatgatatt tctgatttta cccgattaaa aaataatgac atcgtgctca ataaaaaaat
     5041 tgtagatata accggcgtta taaactttgt tatctctcta ttacaaccag cagtagcatc
     5101 aaaagatatt acgattatcc tgtctatcca gagtaatgcc tcccccctgt atgttgatga
     5161 acagcgttta caacagattc ttttaaatat aattggcaat tcgattaaat acaccaaaca
     5221 gggaactatt accattgcca ctcaaatgca tgatagtaat accctttcaa tcataattac
     5281 cagtactgca ggaggcatcc ctcctgaact ggtaaaaaat attgatgatt ttggatatga
     5341 cagcactgta tccaacaaac aatctggcgg atcagctctt ggacttgctg taacaaaaaa
     5401 gctgatagag cttcatgatg gcagcattta cattgagtct caacccgatg gcggttcgtg
     5461 tgttacagta gatttccctt atgatgaaaa tctgattagt aaagaattag caacagcatc
     5521 tacaaatgat tttgtgatta gcttacctga agatatcatc caatatcaac catcgttaat
     5581 gctggatact tcacaaggat ttgtttatat agtagacgac gatcctgtta atgtaaaaat
     5641 tttgcaggag atgctccaga aggccaattt ttacatagaa ttttcccatg atccttcatt
     5701 gctatatgat agtatccaga aaaacagaat acccgatatt gtacttcttg ataccatatt
     5761 gcccggcact tctgcctttc aggtatgtga aacaatacga aaaagctatt cacttcatga
     5821 attgcctatt ataataatga cttcaaaaca ccgaacacag gaaatcatta ccagctttag
     5881 agtaggcgct aacgattacc ttagtaaacc ctataatgaa gaagaattaa tagccagaat
     5941 acataatctt attatgttga aacgatcagt aaaagaacat aatgattatg tgatattaaa
     6001 gcatgaaata aggttagccc atgaaattca taactctgtc gttatacaga atataccaca
     6061 attgcacaac attactattg ctcacgccta catcccaacg cgggacatgg gtggtgattt
     6121 ttatgatgta ataaaaattg atgatgataa aattggtatt atgatcgctg atgtaaccgg
     6181 acatggtatc cccgctgcac tggtatgcgc aatgcttaaa atggcattta tcaacaataa
     6241 agaacatgca cggtatcctg ccaggatgat gacattactc aataaagatt tatccagcac
     6301 aatccatgat aattatatta catcagccta tagtctgatc aacacaaaaa ataaaacaat
     6361 tacagtatca attgcagggc acccccctcc cttattactg cacaacgatg gtacagtatt
     6421 taaaaattgg acaagggcat tccctattgg ctggataggg gacgctaaat atcaagaaac
     6481 aagcatacac taccagcatg gcgataagtt tatattatat actgatggtg ttatagaatt
     6541 gaaaaagggc aaacagatat ttggcgagga taatgtcatt acatgtatag caaacagttt
     6601 tgaaaagcaa ccagctatac taattgatga aatgattaaa ctattacatg attggagctc
     6661 aatccctgct gatgaacctt ttaaagatga tgtaacgcta ctgattgctg aactgttatg
     6721 aaatcatttt ttgatgaaaa atagttattg atttatgaat attgcataat agtaaaatag
     6781 taagaagtat gaattgtaca ataatagaat tacccggtat caaaattgat accattaatt
     6841 ataacagagc agtctgcata ataaaattag ccgatgtcgc ggatatggat aaaaatgatg
     6901 atttacttgc actcacgatg tgtttgataa atggaggctt acgtaaaata atttttgatt
     6961 ttacttcagt tgaatttatt gatagctatg gaattggtac aatagtagaa attgcaaaat
     7021 atattcgcca gcaccataat ggcgatgtag tattaattaa tgtgtccgaa cgtattttat
     7081 taatttttaa accaattcaa ttccagaagt ttatcaaaat atttcattca gaggacgaag
     7141 ccttgcatta ttttcaatac gtttaatcac tataaaatac aggtggttta ctatgagttt
     7201 tgaaatagaa ttattaaatc tttcactaat taacaaggaa aatgttgact atgatatgat
     7261 tattttctac atgaattacc ctgaaaaaat tgatattgac acctcccgcg aaataatgct
     7321 atttttaact acccttatca agggtggggc aaaaaagatc attgttgaca tgaaaaaagt
     7381 tactcttata gacagcgcag gtataagtac acttatttta gctacaaaac aaatcagaaa
     7441 aaacaacggc gatttagttt tatcacatgt taatgattca atccagaaca ttctggatat
     7501 tataaaaatt gaaaaattta ttaaaatttt tcgcactgat agtgatgctg tgcaatactt
     7561 ccgatacatt caaacatagc tgattgttgc ttatggacat atcatttaaa gattattata
     7621 caatacttgg tgtttcccct gctgccgata ctggcaccat taaatcagca ttcagacaaa
     7681 aagcaaaaga aactcaccct gacagcacaa aacaaaacag ctatgcacag tttgtactta
     7741 tccgtgaagc gtatgatatc cttaccaacc cgtcgcagcg agcaagctat gacattgtct
     7801 ggaacaaata tcatgtaaaa tctgaaccag agatgacatc tttatttgat gattttggac
     7861 cacctgatga tcgctatgca caggagtggg aatactttgt attacatcct gaagactatc
     7921 taagccgctt tgagagcaca cttgctttaa taaaggcatc acttagatct attgcaatca
     7981 gcgtctgcat ccccgttata gtgatgataa gtattctttt gggagtaact atcgccatgg
     8041 ttactctttt tgtttttgca atcggaataa catcgtactc ttcgcttatt gctggtattg
     8101 tcataactct attaatggtt aaacggtttg tagaaatttc acagga
//
LOCUS       DAOSOX010000103         8061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8918_length_8061_cov_9.627529, whole genome shotgun sequence.
ACCESSION   DAOSOX010000103 DAOSOX010000000
VERSION     DAOSOX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8918_length_8061_cov_9.627529"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..964
                     /locus_tag="PLX22_08150"
     CDS             137..964
                     /locus_tag="PLX22_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT19908.1"
                     /translation="MQDYLKLFMITVTILLCITEPTFPSDYIQNLKNGTWEQRITAMN
                     TLGYSGNKKAFWYLVKYLDQQFQSTNQNLAIRVRQTAALSLGRLKDDRAIPYLVEQYK
                     KEQDPEVKRAILFALRFFKPNQDTIAIVKQGLSDSNKDIQWEALIVASIYCDSSLAAD
                     VAQLGKDDPEINVISAYIQYMINQNKSENESIMRKSLQHKNPSVRYWASHFLTKSLGT
                     AALEDIMKAQEIESVWWVANEMEDNIYILARKKFNDEMVEQLGSFRFVIDKKDKQVQ"
     gene            complement(971..1486)
                     /gene="lepB"
                     /locus_tag="PLX22_08155"
     CDS             complement(971..1486)
                     /gene="lepB"
                     /locus_tag="PLX22_08155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT19909.1"
                     /translation="METKDSFNIFAKVLFIFAGLIFGFILIRLFFIPVKINNVIMEPA
                     LHNNDYAWFYKTRKVDTGSIVLYKSPLSGKQVGRVIAKSGDTIEIINKRIYVNGIPLH
                     IHWKIVQKDTRTFPPRFTVRDNLAAITVPNGHIFVICDNWDIPTDSRLFGTIPLNAIV
                     GKYIWKLSPKL"
     gene            complement(1508..>2482)
                     /locus_tag="PLX22_08160"
     CDS             complement(1508..>2482)
                     /locus_tag="PLX22_08160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT19910.1"
                     /translation="YMPNAGFTTTRDAIAKKVAKDHHVDIQGKHVIMTCGAAGGLNVA
                     LKTILNPGEEVIVPKPCFAEYQFYISNHNGVITLVSTNADFSLDVDALEKAINDKTRA
                     VLINSPNNPTGVIYSHDDITRLSSLLERYSNKYGKPIYLLSDEPYRELIYDGSTVPSI
                     LSHYNYSMVVSSFSKTMSLPGERIGYIAINPSMPEADIIMAGLILCNRILGFVNAPAL
                     MQRIIERIAFDRVDVNAYAIRRDMFCNGLKGAGYSFITPKGAFYVFCKSPIEDDVIFV
                     KHLLNFNILAVPGVGFGGPGYFRLAFCVPEKVIEKSIPQFKKALEMLK"
     assembly_gap    2483..2582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2675..4495)
                     /locus_tag="PLX22_08165"
     CDS             complement(2675..4495)
                     /locus_tag="PLX22_08165"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT19911.1"
                     /translation="MKVHELKHQTLKKWIQDASNLMKPDDVYVCDGSKDEYDRLAQQM
                     VDEGLAVQLNQKSRPNCLLFRSDPSDVARVENRTFIASKTQEAAGPTNNWIDPVELKQ
                     TMTDLYRGCMRGRTMYVIPFSMGPVGSSIAKIGVEITDSPYVVINMHIMTRVGSKVLD
                     VLGTEGEFVPCWHSVGKPLTKGESDNGKWPCAPLEKKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVMARDEGWLAEHMLILKITNPEGKAKYIAGAFPSACGKTNLAML
                     IPTIPGWKVETVGDDIAWLKFGSDGKLYAINPEAGFFGVAPGTSEESNYNAMKTIEKN
                     TIFTNVALTEDFNVWWEGIGYEPKGKLIDWLGNEWIYDKNNKDQKPAAHPNSRFTTPA
                     NQCPSIAKEWEDPDGVPISAILFGGRRAQTIPLVVESKNWLHGVFLGSIVGSEVTAAA
                     LDLTIGQVRRDPFAMLPFCGYHMGDYFKHWINIEKKTTSEKLPKIFFVNWFRKTDDGK
                     WLWPGYGENSRVLKWIFERCDGSGKAVDTPVGYMPAPDAIDKPASVSNQDMEILLKVD
                     REGWLREVDDVRNNHYPKFGNHLPQELYELLDTMESLLKA"
     gene            complement(4647..4949)
                     /locus_tag="PLX22_08170"
     CDS             complement(4647..4949)
                     /locus_tag="PLX22_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19912.1"
                     /translation="MVYSFDKDKTGYFLKIEGDLDLYSSYNLKEDIKAIFLKEPENII
                     IDLSHLNYIDSAGIGFLIDIKKNATQNNKLCDFKNIPSDILKLFHSIRVDVILHIK"
     gene            complement(5144..6061)
                     /locus_tag="PLX22_08175"
     CDS             complement(5144..6061)
                     /locus_tag="PLX22_08175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT19913.1"
                     /translation="MMYINDPGPYHSNTLTTLGWELTVCNMLYPENSPCRAVTKHNAS
                     FGDTLYYFLSDHINFQDIHAIMEVGGGYGFLMKDFIKHCNAARVTMVDISPVMLAEQK
                     KNLDGDITCIQSDFLKLDDAIVASNELILCNEAIGDFITLVNVTKSTIAHSDVPVLRE
                     CNRLIHEYSLTSDEEIFNFNLGALLAMEKMCRAGVKAIYVSEHSCESMAYGEFKDCID
                     VTPTNNPQQINLYGHSEYTIKFSHLVAIAKRWGYTIYRGVYNTLFQCAMTPQLRFILN
                     STVNSRDEHEILRQFMGDMFTYEYIVCLR"
     gene            complement(6058..6438)
                     /locus_tag="PLX22_08180"
     CDS             complement(6058..6438)
                     /locus_tag="PLX22_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19914.1"
                     /translation="MYVIKPCPNCGAKLRFPINSGTVKVTCKCGYSFIANPDNPRLYS
                     DAVFDLWYDSKPKPKASQTSIISRVIEKLYSYWYTILNFRLLPTKTQRTIIVIIIIII
                     LLVMALFYLLFSSPPQPPSGGITV"
     gene            complement(6449..7309)
                     /locus_tag="PLX22_08185"
     CDS             complement(6449..7309)
                     /locus_tag="PLX22_08185"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT19915.1"
                     /translation="MHEHIQQRIQSIIQNVEQLPSLPDVVSKIINMVNDPDVSFKHVA
                     DEIAKDQAITANILKLCNSAYFSKGKEISSIDRAIVILGLKEVKDIVVIATTKSVLNK
                     VIVGYDLARGELWKHGVAVAMLAKKIATECNQKTIADIAFTGGIIHDVGKTVLALFVQ
                     STFKDILNTVTEKSITFQEAEKVVMGFDHQQIGEQVAIKWKFPMVLQSIVRYHHEPMN
                     APDDHKMIVSIVHVANTLCLMAGIGIGSDGLYHELNYDAIKLLSLKDSELEKLFADIP
                     ELMMKAKDIL"
     gene            complement(7332..7811)
                     /locus_tag="PLX22_08190"
     CDS             complement(7332..7811)
                     /locus_tag="PLX22_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOT19916.1"
                     /translation="MDNLLNIGVAQFTVAENPVIMRTILGSCVGICVYERNKKIGGLA
                     HILLPKNPGNANPEKYADTAIPMLIQQMLKKGCKREFMTAKIAGGASMFKFKSTTTLA
                     NIGENNVEATRQALAKFSIPIVAEDIGGSAGRIIDFFLEDGRLKVKASGDEKIYYKV"
BASE COUNT         2475 a         1631 c         1314 g         2541 t
ORIGIN      
        1 gattttatta ataattatta aaattgtcaa taaacttgca aatgtttaaa aaaaaattta
       61 aaaaataatt gagtttttta tggtgatgtt gtgaaatttt ttataaatat ctattctatt
      121 ctggagcgta ccaattatgc aagactattt aaaacttttt atgattactg tcactattct
      181 gttgtgtata acagaaccaa cttttcccag cgattatatc cagaatctaa aaaacggcac
      241 atgggagcaa cgaatcacag ccatgaacac actcggatat tcagggaata aaaaagcctt
      301 ctggtatctg gtaaaatatc tggatcaaca gttccaatca actaatcaaa atttagctat
      361 acgggtacgc cagactgcag cactgtcttt aggcaggctt aaagatgatc gcgccattcc
      421 ctatcttgtt gaacaataca aaaaggaaca ggatcccgag gttaaaaggg ctatcctttt
      481 tgctctccgt ttttttaaac caaatcagga tactattgct attgttaagc aggggctgtc
      541 cgacagcaat aaagatattc agtgggaagc tcttatagtt gcaagtatct attgtgattc
      601 gtcgttagca gcagatgttg cccaattagg caaggatgat cctgaaatta acgtaatctc
      661 tgcatacatt cagtatatga ttaatcaaaa caaatccgaa aacgagtcca tcatgcggaa
      721 atcattacaa cacaaaaatc cttccgtacg ctattgggct tcacactttt taacaaagtc
      781 attgggtaca gcagcacttg aggatattat gaaagctcag gaaattgaat cggtgtggtg
      841 ggttgcaaac gagatggaag ataatatcta tatccttgca cgcaaaaaat ttaatgacga
      901 aatggttgaa caactgggtt cattcaggtt tgttattgat aaaaaggata agcaggtaca
      961 ataagtttaa ctataatttt ggcgatagtt tccagatata ctttccaaca atagcattaa
     1021 gcgggatagt accaaacaat ctgctgtcgg tagggatgtc ccaattatca caaatcacaa
     1081 atatatgccc attggggaca gttatcgcag caaggttatc tcgcacagtg aatcgtggcg
     1141 ggaacgtcct ggtatctttt tgcacaatct tccagtgtat atgcaaagga atgccgttaa
     1201 catatatcct cttattaata atctcaatgg tatcaccaga ttttgcaata accctgccaa
     1261 cctgtttacc tgaaagggga cttttgtaga gaactatcga acctgtatca acttttcttg
     1321 ttttataaaa ccatgcataa tcattattgt gtaatgccgg ttccataatg acattattaa
     1381 tttttacagg aataaaaaac aaccggataa gtataaatcc aaaaattaaa cctgcaaaaa
     1441 taaacaatac ctttgcaaaa atattaaacg aatcttttgt ctccatatgt attgctactt
     1501 ttttatctta tttcagcatt tcaagtgctt ttttaaactg aggtatggat ttttctatta
     1561 ccttttcagg cacgcaaaag gctaatctaa aatagccagg tccgccaaat ccaaccccgg
     1621 gcactgcaag tatattaaaa tttaaaagat gctttacaaa tataacatca tcttctatgg
     1681 gcgatttgca aaaaacataa aaagcgcctt taggggtaat aaaactatat ccggcacctt
     1741 tgagaccatt gcaaaacata tcccgccgaa ttgcatatgc attgacatca accctgtcaa
     1801 aggctatcct ttcaattatc cgctgcataa gagcaggggc attgacaaat cccaatattc
     1861 tattacacag tattagtcct gccataataa tatctgcttc gggcattgat ggatttatgg
     1921 caatgtatcc aatccgttca ccaggaagtg acattgtttt tgaaaaagaa gaaacaacca
     1981 ttgaatagtt ataatgtgat aaaatactgg gaactgtact gccatcatat atcagctctc
     2041 tatatggctc atctgataat aggtaaatag gcttcccata tttatttgaa tagcgttcca
     2101 acagtgatga caatcgtgtg atatcatcat gcgaataaat aacaccggta gggttgttgg
     2161 gagaatttat taaaaccgct cttgttttat cattgattgc tttttccagg gcatcgacat
     2221 caagtgaaaa atcagcattg gtggatacta atgttattac tccattatgg ttggaaatat
     2281 aaaattgata ttctgcaaaa cagggttttg gtactatgac ttcttcacca ggatttaata
     2341 tggttttcaa agctacattg agccctcctg ctgcaccgca tgtcataata acatgtttcc
     2401 cctgaatatc aacatgatga tcttttgcca cctttttagc aatagcatct cgtgttgttg
     2461 taaatcctgc atttggcatg tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnggatatgt atttgtgttt gttaaaaatg gggctgtaaa aaattaactt ttagacagcc
     2641 ccttacatga tacaatggaa tgtatagcaa cttttcaagc tttgagaagt gattccatag
     2701 tatcaagtaa ttcatatagt tcttgcggca ggtgattgcc aaacttaggg taatgattat
     2761 tccttacatc atctacctca cgcagccacc cttctctgtc aacttttaac aatatttcca
     2821 tatcctgatt tgatacagaa gccggcttat caatggcatc aggagcgggc atatagccaa
     2881 caggagtatc aactgcttta ccactaccat cacatcgctc aaatatccat ttcagcactc
     2941 tgctattttc accatatccg ggccatagcc atttgccatc atcggttttt ctgaaccagt
     3001 taacaaaaaa tatctttggc aacttttcag atgttgtttt cttttctatg tttatccagt
     3061 gtttaaagta atctcccata tggtatccac aaaacggaag cattgcaaat gggtccctgc
     3121 gcacctgtcc aatggtaaga tccaatgccg ctgcagttac ttcagaacca actatcgaac
     3181 ctaaaaacac accgtgcagc caatttttag attccaccac caatggtatt gtttgtgcgc
     3241 gtctaccgcc gaataatatt gcactgattg gtaccccgtc tggatcttcc cattcttttg
     3301 cgatggatgg gcattggttt gctggggtgg taaatcgtga attaggatga gcagcaggtt
     3361 tttgatcttt attgttttta tcatatatcc attcattccc aagccagtca attagtttgc
     3421 ccttgggttc atatcctata ccttcccacc acacattaaa atcttcagtt aatgcaacat
     3481 tagtaaatat tgtattcttt tctattgtct tcattgcatt atagtttgat tcttcagaag
     3541 ttcctggtgc aacaccaaaa aatcctgctt caggattaat cgcatacaac tttccatcag
     3601 acccaaattt aagccatgcg atatcgtccc caacggtttc taccttccac ccgggaatgg
     3661 ttggtataag catagcaaga ttggttttac cacatgccga tggaaatgct cctgctatat
     3721 attttgcctt tccttcagga ttggtaattt ttaatatcag catatgctcg gcaagccacc
     3781 cttcatcgcg tgccatgacc gaagcaatgc gcaaagccaa acactttttc cctaacaacg
     3841 cgttgccacc ataccctgag ccataagacc agatctcacg tgtttcagga aaatgcgaga
     3901 tatacttttt ttctaatgga gcgcatggcc atttaccatt atcactttct ccctttgtta
     3961 atggtttccc aactgaatgc cagcacggaa caaactcccc ttcagttccc agaacatcaa
     4021 gcaccttact gccaacacgc gtcataatat gcatattaat aacgacatat ggcgaatcag
     4081 taatctctac tccaattttt gcaatggatg agccaacggg gcccattgaa aacgggatga
     4141 catacatggt tcttccacgc atacatccac ggtaaagatc agtcattgtt tgttttaatt
     4201 ctaccggatc aatccagtta ttagttgggc cagcagcttc ctgcgttttt gatgcaatga
     4261 acgtcctgtt ttccaccctg gctacatcgc ttggatcaga tctaaaaagt aaacagtttg
     4321 gtcgtgattt ttgatttaac tgcactgcca aaccttcatc aaccatttgc tgagccaacc
     4381 tgtcatattc gtccttgctt ccatcacaca cataaacatc atctggtttc atcaaatttg
     4441 atgcatcctg aatccacttt ttcaatgttt ggtgttttaa ctcatgtacc ttcataatgc
     4501 ttcctttcta gtattaaagg attataaaac tattgtgagt aacggttatg gtgtacaatt
     4561 tttttgtcaa ctattactat tcaaaatagt agcgaccaat ctattaacca aattgtaaca
     4621 aaattgattt aataataaaa aatagtctat tttatatgaa gaataacatc gacacgtatg
     4681 gagtggaata actttaaaat atcagatggg atgtttttaa aatcgcatag cttattattc
     4741 tgagtggcat ttttctttat atcaattagg aaacctattc ctgctgaatc tatgtaattt
     4801 aaatgcgata gatcaataat tatattttca ggttccttta aaaatatagc cttaatatct
     4861 tcttttaaat tgtaagaaga gtacaggtca agatcaccct caattttaag aaaatatcct
     4921 gttttatctt tatcaaatga atataccata cactatcact ttcagagctt tttaccacac
     4981 tataattatc gtattataaa agataatagt tgaatcaatt cgtccagtac cccaataaat
     5041 agcctgctat ataatgtgtt tatattatca ctattagaat atatagcaat atcctcaata
     5101 aaatcacata aaaaactact gatataaaat aactacacag taactatcgc aaacatacaa
     5161 tatattcgta ggtaaacata tcccccataa actgtcgcaa gatttcatgc tcatccctgc
     5221 tattgactgt actgttaaga ataaatcgca attgaggtgt catagcgcat tgaaatagcg
     5281 tattatacac gcctctatag attgtatacc cccaccgctt tgctattgct accagatgtg
     5341 aaaatttgat ggtatactcg ctgtgcccat agaggtttat ttgctgcgga ttatttgttg
     5401 gagtaacgtc aatacaatct ttaaattcac catatgccat agattcacag ctatgttcac
     5461 tgacatagat agcttttacc ccggccctgc acatcttttc catggcaagg agcgcaccta
     5521 aattaaaatt aaaaatttcc tcatcacttg tcagtgaata ttcatgtatc aatcggttgc
     5581 attcccgtaa taccggtaca tcactatgag cgatagttga tttggtaaca ttcaccagtg
     5641 tgataaaatc acctatagct tcattacaca gtataagttc gttacttgct acgattgcat
     5701 catcaagttt taaaaaatct gattgtatac atgtgatatc accatctaaa tttttctttt
     5761 gttcagcaag catcaccgga gatatatcca ccatggtgac acgggcagca ttacaatgct
     5821 ttataaaatc tttcattaaa aatccgtagc caccacccac ctccatgatt gcatggatat
     5881 cctgaaaatt gatgtggtca ctcaaaaaat aatataatgt gtcaccaaac gatgcattgt
     5941 gcttggtcac agcacggcag ggactatttt caggatagag catgttacaa acggttagtt
     6001 cccatcccaa tgttgttaat gtattactat ggtatggccc cggatcatta atgtacatca
     6061 taccgtaatc ccacctgaag gaggctgtgg tggtgatgaa aagagtaaat aaaaaagtgc
     6121 cataacaaga agaattataa ttataataat tacgataatt gttcgttgcg tttttgtagg
     6181 cagcaatcta aagttcagga ttgtgtacca gtacgaatac aatttttcaa tgaccctgct
     6241 tattatactg gtttgggatg cttttggttt tggtttacta tcataccaca gatcaaatac
     6301 tgcatccgaa tagagccggg gattatcagg attagcaata aacgaatatc cgcatttgca
     6361 ggtaactttt acagtaccgg aattaatcgg gaaacgcaat tttgctccac agttggggca
     6421 gggttttatg acatacatac ctgataccct acaggatgtc cttagctttc atcatcaact
     6481 cagggatatc agcaaaaagc ttttctaatt cggaatcttt gagtgataac agcttaattg
     6541 catcataatt taactcatga tataaaccat cactaccaat cccaatcccc gccattagac
     6601 acagcgtatt ggcaacatga actattgata ctatcatttt atgatcatca ggtgcattca
     6661 tgggttcatg gtgataccgt acaatagatt gcaaaaccat ggggaacttc cactttattg
     6721 caacctgctc accaatctgc tggtgatcaa aacccatgac taccttctca gcttcctgga
     6781 atgtaatgga tttctctgtc acagtattga gtatgtcctt aaaggtactt tgaacaaaaa
     6841 gtgcaagtac tgttttccca acatcgtgta tgatgccgcc agtaaatgct atgtcagcaa
     6901 ttgttttttg attacattct gtggcaattt tttttgcaag catagcaaca gcaactccat
     6961 gtttccataa ctcgccccgt gccagatcat agccaacaat cactttattc agcactgatt
     7021 ttgttgttgc tatcactaca atatccttaa cctctttcaa accaagaatg acaatggcac
     7081 gatcaataga tgaaatctcc ttgcctttac taaaatatgc tgaattgcaa agctttaata
     7141 tattggcagt aattgcctgg tctttggcta tctcatcagc tacatgttta aacgaaacat
     7201 ctggatcatt taccatattt atgatcttgc ttacaacatc tggcaacgat ggcaactgct
     7261 caacattctg aattatggac tgaatccttt gttgtatatg ttcatgcata gttatttctc
     7321 caatccatca atcaaacctt atagtatatc ttttcatcgc cgctggcttt tacctttaac
     7381 cggccatctt ctaaaaaaaa gtcaattatc ctgccagcac tgccaccaat atcctcagct
     7441 actatcggta tactaaattt agcaagcgcc tgtcgtgttg cttcaacatt attttcgcct
     7501 atatttgcca atgttgttgt agacttaaat ttaaacattg atgcaccacc ggcaatcttt
     7561 gctgtcataa attccctttt gcagcctttt ttcaacatct gctgtattag cataggaatt
     7621 gcagtatctg catatttttc aggattggca ttaccaggat ttttgggcag taagatatgt
     7681 gccagccctc caatcttttt attgcgctca taaacacaaa taccaacaca actccccaat
     7741 attgttcgca ttattacagg gttttcggcc acagtgaatt gtgcaacacc tatgtttaaa
     7801 agattgtcca tccttattcc cgctatcaaa aatatacaga atgaaaaatt attatcttga
     7861 ttaattatga ctgcctgtac atatttgtca aggcaaaaga tatattttac gtaaataatc
     7921 acgtaaataa taaaattaga tgtcaacaac agttattaac agtatgctga acgctatccc
     7981 atccaatgct gccaataaaa caggtggcat atatattaat attgttaaaa atgaatttat
     8041 aatccttgat aacgaaatta a
//
LOCUS       DAOSOX010000104         8034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_8956_length_8034_cov_8.493420, whole genome shotgun sequence.
ACCESSION   DAOSOX010000104 DAOSOX010000000
VERSION     DAOSOX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_8034_cov_8.493420"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1129)
                     /locus_tag="PLX22_08195"
     CDS             complement(362..1129)
                     /locus_tag="PLX22_08195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT19917.1"
                     /translation="MILGFNIGNTNTVMGIYDEDNPEPVYATTYPTRQTIQSDELSHI
                     IYNTLKQWDKRLASSITGVIYSSVVPQSNTTYNKTIEELFKVTVHSITHLSKMPIRIE
                     YDNPAQLGVDRIVNAVAAFTDYACDCIIVDIGTAITFCQLTQGVLRGGIIAPGVKVAM
                     KGLAQSTAQLFEVPFEKPPYIIATNTSDALISGFFYGWIALIEGIIGKIQSTMGTCMV
                     IVTGGMAEILYPHLSISAIVDHSLTMRGIFYLYNSNR"
     gene            complement(1144..2169)
                     /locus_tag="PLX22_08200"
     CDS             complement(1144..2169)
                     /locus_tag="PLX22_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT19918.1"
                     /translation="MALFTPNIDKLREKNDIQGLLKLTKHRKAEVRLNALLALVKTRD
                     QSVTQELKKLINDPDPRVRTIATIKFGDINDPAIIENLRSIIISGSQRDKIEALRVLS
                     EIGPTDDLEISKILYLAFKDKKMMVSIEAIRTMGYLKDKYAIPHLVEALDSKHYQIRV
                     EATRALGMIATEQAIDPLIGCLVDNHAEVRKAAHDALKKIGTDKALKAITDAPFMLLV
                     KHMTEGGSQREETVKQIGQYRIKEAAPLLIKACNDEFKSVRAAAARSIGILREKSAVN
                     ALTKLLNDPYYDVRLEAVRALEKIFDPQALKAIEKAMSDSNKNVRDEARVAYYSLKSR
                     LEKVYET"
     gene            complement(2273..4015)
                     /gene="selB"
                     /locus_tag="PLX22_08205"
     CDS             complement(2273..4015)
                     /gene="selB"
                     /locus_tag="PLX22_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOT19919.1"
                     /translation="MYVIGTAGHVDHGKTSLIKALTGIDADRLPEEKQRHMTIDIGFA
                     YITLPAIGMVSIVDVPGHERFIRNMVTGAWGIDIGLLVIAADDGWMKQTDDHVKVLSI
                     LGIPAMVVALTKIDTIEQQTLPQVLSHINAMLATTPYRQAPVVPCSSITGEGITALKE
                     ALTNAIIKLPVPTTGNKPYLHVDRVFTVKGAGTVVTGTSKNGSFALNDTITIVPHATK
                     ARIRQIQSHNTQQQQTDVSQRTALALAGVEKTTLQRGDIIVRENFFTSATHCIAYITH
                     TFTQIKNNQWIEVLIGTASYDAQYTMISDSKPIVRLKCKQAVWLYPSQRFVATLPGGH
                     AIIAGGYILCHNYSALIKKDPVIHAITNNNSIEANAILCIVACNPFITNHALKKLFPD
                     ESAVAKAIQLPVQQSLLMLIDDIVLTQFQYDTAVKLLKQYAGTDTTTVDIAQRLSIPH
                     NLVNHIMAQYGYGKKLNGEMSDAEKHLLQQLLQKGIEGINPKDFQRHKNILDKLIKSE
                     NAIVIDRELLWHRDVFLHYAESIMAHFNTNKELTVQQAKEITGLSRKYVIPLLNALEQ
                     KKFIKRYGDVRLKA"
     gene            complement(4025..5359)
                     /gene="selA"
                     /locus_tag="PLX22_08210"
     CDS             complement(4025..5359)
                     /gene="selA"
                     /locus_tag="PLX22_08210"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOT19920.1"
                     /translation="MYKQIPQVQKLLEEEAIQPFITTIGHPFVVSAIRDTLDMERKNI
                     ADGKPFDYASLIGAIVARCRAISLEKLQRVINGTGVLLHTNLGRAPLGKEVLQSIAQQ
                     LYGYCNLEYTIPTQKRGKRGGFAEKLICTITGAEDALIVNNNAASIFLILHHFAHGKE
                     VVISRGELIQIGGGFRIPDIMQQAGCTLVEVGTTNITRQNDYIKALTPNTAIILSAHR
                     SNFYMKGFTDSPTLQQLAALKTDSLLLVKDLGSGNFIFNDEDNVSQALNHADIVCFSG
                     DKLLGACQAGIIAGKKALIEELRNNPLMRIMRVDKITYFIIQEVLLRYANHQHCTLPV
                     WDMMKQDVTIIDKKIKKLMKKLNADIKPYIHHTASKTAFGGGAMPHKVLPDYGIIIAI
                     DGKRAGDIAHHMVHQPVPIIGYIQNDAYTLNFRTIDDNDIASIADAINSLLP"
     gene            complement(5426..5517)
                     /locus_tag="PLX22_08215"
     tRNA            complement(5426..5517)
                     /locus_tag="PLX22_08215"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5481..5483),aa:Sec,seq:tca)
     gene            complement(5575..6270)
                     /locus_tag="PLX22_08220"
     CDS             complement(5575..6270)
                     /locus_tag="PLX22_08220"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT19921.1"
                     /translation="MQPLKKFYEENLYPLQDGILNCVKNLNLPFYLTGGTALSRGYFN
                     HRYSDDIDLFTNSNPDFKSHSDAIVLTLKNNTYAIDTSTMILSQDYVSFYVSCEGLSN
                     IQCKLDLVNDKAPHFGIIQPTPVYYKTDDWYNILINKFTALFRLEIKDFVDIWIIAKN
                     KKFNWDDIITKGREKELGLDAIVCAHLMKTISFELLPTIKWAIDVSFDEFLKDMDTIA
                     IDLLKGCDNSLVT"
     gene            complement(6152..6526)
                     /locus_tag="PLX22_08225"
     CDS             complement(6152..6526)
                     /locus_tag="PLX22_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19922.1"
                     /translation="MKYSDDKRRDLIKKIFWDYEIDIESLLNHIDNGTLPKAKEQKLF
                     IRCLEHLPWHCVVGVFGFDKAKELLTDEVIQKVWPEERSDHFATLKKILRGEPLPTTR
                     WDSELRKKLKSTVLSYRWYRTQ"
     gene            complement(6538..7521)
                     /gene="selD"
                     /locus_tag="PLX22_08230"
     CDS             complement(6538..7521)
                     /gene="selD"
                     /locus_tag="PLX22_08230"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOT19923.1"
                     /translation="MGPADLDAIVKKLPITYTGNVLVGMQTGDDAGVYKISDDVAIVQ
                     TVDIITPIVDNPFVFGKIAAVNSISDIYAMGGTPITALNIVCFPVTTFTMDVLEKILL
                     GGLQTLQQTGIQLIGGHSIDDVELKYGLAVTGLIHPDAIITNTGIQPDDVIILTKPLG
                     TGTINTAIKAGVADSNHIDECISSMTTINDCMAGYPYPEHIHAMTDVTGFGLIGHLNE
                     MLGDSSLEIVINTEAIPFLNGATRYAAMGLLPGGLYRNRDFAGKRCTIHSSVKREIAD
                     LLFDPQTSGGLLIAVSPDNAGKVIDYSAKNALCTAIIANCRKADSAHISIE"
BASE COUNT         2205 a         1596 c         1659 g         2574 t
ORIGIN      
        1 caaaacaata attatggcaa tacaattttt atttgtcacg ctatccacaa aattgaaaaa
       61 ttaacagaaa ttaatttatg ttaatttgca atagaatagt cataaaaaaa tttttaaaaa
      121 aaaacaaaaa atatttataa aaatatattg caatgaaact cctcgtattt ccagcattta
      181 tataaggctc aatcaaaaaa tttactgata tttcagtgta gtaatgtgac atagtaatcg
      241 tgtagcagcg ataagccaat taatttagta aattaatgtc atgagatgct gtcaatgtgg
      301 atagtgtgga taactttgtg aatattaata tagtaggact gttttattta tgtttacacg
      361 cttagcggtt actgttatac aaataaaaaa taccacgcat ggttaatgaa tggtcaacaa
      421 tagctgagat tgaaaggtgc ggatagagta tttcagccat cccaccggtt acaatgacca
      481 tgcaggtgcc catcgttgac tgaattttgc ctataattcc ctctataagc gctatccagc
      541 cgtaaaaaaa gcctgaaatg agggcatcgc tggtattagt agcaataata tagggtggtt
      601 tttcaaaagg tacttcaaat aattgagcgg tagactgtgc taaacccttc attgctacct
      661 ttacgcctgg tgctattatt cctccccgta ataccccttg tgtaagctgg cagaaggtaa
      721 tggcagtacc aatgtccact atgatgcaat cgcatgcata atcggtaaaa gcagcaacgg
      781 cgtttactat cctatccacg cctaactgtg caggattgtc atattctatc ctgattggca
      841 tttttgaaag gtgtgttatg ctatgaactg tcaccttaaa aagttcttca atggttttat
      901 tgtatgttgt gttggattgt gggaccacgc ttgaatagat aacaccggta atgctggacg
      961 caaggcgttt atcccattgt ttcaaagtat tatatataat gtgcgatagt tcatcggatt
     1021 gtattgtttg tcgggtaggg taggtagtgg cgtatactgg ctctggatta tcctcatcgt
     1081 aaatgcccat aacggtattg gtattgccaa tattgaagcc aagtatcatg aagcaatcct
     1141 gatctacgtt tcgtaaactt tttccagccg ggatttaagg gaataatacg ctacgcgcgc
     1201 ttcatcacgg acatttttat tggaatcgct cattgctttt tcaatggctt tcagcgcctg
     1261 aggatcaaat atcttttcta aagctctgac agcctcaagg cgcacatcgt agtagggatc
     1321 gtttaatagt ttggtgagcg cattaacagc acttttttcg cgtaatatgc caatagaacg
     1381 ggcagcagcc gcacgaactg atttaaattc atcattgcag gctttaataa gaagaggtgc
     1441 ggcttctttt atcctgtact gaccaatctg ctttaccgtt tcctcgcgtt gcgagccgcc
     1501 ttcggtcatg tgttttacca gcagcataaa tggtgcatcg gtaatagcct tgagcgcctt
     1561 gtcggttcca attttcttca gagcatcatg agcagctttg cgcacttcag catgattgtc
     1621 aacaagacat ccaattaacg ggtcaattgc ctgctcagtt gctatcatac ccaatgctct
     1681 ggttgcttca acccttatct ggtaatgttt gctgtccagt gcttcaacca gatgaggtat
     1741 ggcatactta tcttttagat atcccattgt gcgtatagct tcaatggaaa ccatcatctt
     1801 tttatctttg aaggctaaat ataaaatttt tgatatttcc aggtcatccg ttggacctat
     1861 ctcgctcaat acacgaagcg cttcaatttt atcgcgctgt gaacctgaga tgattattga
     1921 gcgtagattt tctattattg ccgggtcatt aatatcacca aattttatgg tggcgatagt
     1981 tctaacacga gggtcagggt catttatgag tttttttaat tcttgagtaa ctgattgatc
     2041 gcgggtttta accagtgcaa gcagtgcatt gagacgtact tctgctttac ggtgttttgt
     2101 taattttaaa agcccctgaa tatcattttt ttcccttagt ttgtctatgt tcggagtaaa
     2161 aagtgccata gcaattcctt taattacttt tattttttac aagtatagtt accatgtaca
     2221 aaaaaagtca actaataatg tacaatttta ttttatcatg caaattaaat agttatgctt
     2281 tcagccgtac atcgccatag cgtttaatga attttttttg ttccagcgca ttgagtagcg
     2341 gaataacata tttccttgac agtcctgtta tttctttagc ctgctgtacg gttaattctt
     2401 tattggtgtt aaaatgtgcc atgatgcttt cagcatagtg taaaaaaaca tccctgtgcc
     2461 acagcagttc cctgtcaatg acaattgcgt tttctgattt tataagctta tcaagtatat
     2521 ttttatgacg ttgaaagtcc ttaggattaa tgccttctat gcctttttgc aatagctgct
     2581 gcagcaggtg cttttcagca tcgctcattt caccatttag ttttttgcca tagccatact
     2641 gtgccatgat gtggttaacc agattgtgtg gaatggaaag cctttgtgct atatccacgg
     2701 tagttgtatc agttccggca tattgtttca gcagctttac ggcagtgtca tactgaaact
     2761 gagtaagcac tatgtcatca atcagcatca gcagtgattg ttgtacaggt aactgtattg
     2821 ctttagcaac agcactttca tcagggaaaa gttttttcag cgcatggttt gttataaaag
     2881 gattgcatgc aacaatacac aaaattgcat ttgcttcaat gctgttgttg tttgttattg
     2941 catggataac gggatctttt tttatcaacg ctgaatagtt atgacatagt atataaccgc
     3001 ctgcaataat agcatgcccc cccggcaacg ttgccacaaa acgttgtgaa ggatacagcc
     3061 acaccgcttg cttacattta agccttacta tcggcttgct atcgcttatc atggtgtatt
     3121 gagcatcgta tgatgcagtg ccaattaata cctcaatcca ttgattgttc ttaatctgcg
     3181 taaatgtatg cgtaatgtat gctatgcagt gtgtggctga tgtaaaaaaa ttttccctga
     3241 caatgatatc accgcgctgt aaggttgttt tttcaacacc tgcaagcgcc agtgcggtgc
     3301 gctgtgatac atccgtttgc tgctgctgtg tattgtggct ttgtatctgg cgtatgcgcg
     3361 cttttgtggc atgaggcact atggtgatgg tatcattgag tgcaaagctt ccatttttgc
     3421 tggtgcccgt aacaaccgtg ccagcacctt tgacggtaaa aaccctgtca acatgcaggt
     3481 acggtttgtt gccggtagtg ggcactggca gttttataat tgcattggtt aaggcttctt
     3541 taagagcagt aattccctct cccgttatgg acgagcaggg tacaacaggt gcttgccggt
     3601 aaggagtggt agcaagcatg gcatttatgt gcgatagtac ctgtggaagg gtttgttgct
     3661 ctatagtatc aattttggta agtgcaacaa ccattgccgg tatccccagt atggataaaa
     3721 cctttacatg atcatcagtc tgtttcatcc agccgtcgtc ggcagcaata accagaagcc
     3781 caatatcgat gccccatgct ccggttacca tattacggat aaagcgttca tgtcccggca
     3841 catcaacaat acttaccatg ccaatggcag gaagggtaat atatgcaaaa ccaatgtcga
     3901 tggtcatgtg gcgctgcttt tcttcgggga gtcggtcggc atcaatcccg gtaagtgcct
     3961 tgatcagcga tgttttacca tggtcaacat gtccggcagt tccaatgaca tacataccgt
     4021 gtacctatgg caataatgag ttgattgcat cagcaatgct ggcgatgtca ttatcatcga
     4081 tggtgcggaa atttaaggtg tacgcgtcat tttgaatgta gccaatgatg ggcaccggct
     4141 gatgtaccat gtgatgtgct atatcaccgg cacgtttacc gtcaatagca atgattatac
     4201 cataatcggg aagcaccttg tggggcatag cgccgccgcc aaatgccgtt tttgaagcgg
     4261 tatggtgtat atatggttta atatcagcgt ttagtttttt cataagcttc tttatttttt
     4321 tatcaatgat agtaacatcc tgtttcatca tatcccatac cggcagggta caatgctggt
     4381 gattggcata ccgtagcaat acctcctgga taatgaaata ggttatttta tccacacgca
     4441 tgatgcgcat caatggattg tttctcaact cttcaattaa tgcttttttg ccggcaataa
     4501 tgccagcctg gcatgctcct aacagcttat caccgctaaa acaaacaata tcagcatggt
     4561 ttaatgcctg tgaaacattg tcctcatcat taaagataaa atttccgctg cccagatcct
     4621 ttaccagcag cagcgaatcc gtttttaatg ctgcaagctg ctgcagggta gggctatcgg
     4681 taaatccctt catataaaag ttggagcggt gagccgacag gatgattgca gtattggggg
     4741 tcagagcctt tatataatcg ttttgccggg taatgttggt tgtgcccacc tcaacaaggg
     4801 tacaacctgc ctgctgcatg atatcaggga ttctaaatcc accgccaatc tgtatgagct
     4861 cgccacggga tataaccacc tctttaccat gtgcaaagtg atgaagtatc aaaaatatgc
     4921 tggcagcatt gttgttgacg atgagcgcat cttcggcgcc ggttatcgtg catataagct
     4981 tttcggcaaa gccgcctcgt ttaccgcgtt tttgcgttgg gatggtatat tccagattgc
     5041 agtatccata aagctgttga gcaatgctct gcagtacctc tttgcccaat ggagcccgtc
     5101 ctaaattggt gtgcaacaaa acgccggtac cattaatgac gcgttgcaac ttttcaaggg
     5161 atattgccct gcaccgggca actatcgccc caattaatga tgcataatca aatggcttgc
     5221 catctgcaat atttttccgc tccatgtcaa gagtatcacg aatagcagaa accacaaaag
     5281 ggtgaccgat agttgttatg aacggctgta ttgcttcttc ttctaaaagc ttttgtacct
     5341 gaggaatttg tttgtacata tctgccatcc tgtgcaatga aaaccttaca gatgagcaag
     5401 cagcaaggtt ttgcctattt taaaatgcgg aaggggcagg aatcgaacct gccgctgccg
     5461 gtaagggcag ccagcggttt tgaagaccgc agagaccacc agatctcatc cgcttccaat
     5521 acaaaaatat atggttacta taacagaatg tcaaaaacaa attgaaaaaa tattttatgt
     5581 aacaagtgaa ttatcacatc ctttcaaaag gtcaatagca attgtgtcca tatcttttaa
     5641 aaattcatca aatgaaacat ctattgccca tttaatagta ggtaataatt caaatgaaat
     5701 tgttttcatt aaatgtgcgc atactattgc atctaatccc agctcttttt cacgcccctt
     5761 tgtgattata tcatcccaat taaatttttt attttttgca ataatccaga tatccacaaa
     5821 atcttttatc tctaatcgga atagtgcggt aaatttattg attaaaatat tataccaatc
     5881 atcagtttta taatagactg gcgtaggctg gattatgcca aaatgtggtg ctttatcatt
     5941 cactaaatca agtttacatt gaatgttgct taacccttca catgaaacat aaaacgatac
     6001 ataatcctgc gacagtatca ttgttgaagt atcaatggcg tatgtattat tttttaatgt
     6061 aagtactatt gcatcactat ggcttttgaa atctggatta ctgttggtaa ataaatcgat
     6121 atcatcagaa tatcgatgat taaaatatcc tctactgagt gcggtaccac cggtaagata
     6181 aaacggtaga tttaagtttt ttacgcaatt cagaatccca tcttgtagtg ggtagaggtt
     6241 ctcctcgtag aattttttta agggttgcaa aatgatcact cctttcttca ggccatactt
     6301 tttgtatcac ttcatcggtt aatagctctt tagctttgtc aaagccaaaa acacctacaa
     6361 cgcaatgcca tggaagatgt tccagacagc gaataaatag cttttgttct tttgctttgg
     6421 gcaaagtacc attgtcaatg tggtttagca aggattctat atctatttca taatcccaga
     6481 atattttttt aataaggtct cttcgtttat catctgaata tttcattgtt cagtactcta
     6541 ttcaatagaa atatgggcac tgtcagcctt gcggcagtta gctattattg ctgtgcacag
     6601 ggcgtttttt gcactgtagt caatgacttt gcctgcatta tcaggcgata cggcaatcaa
     6661 aagcccgccc gatgtttgcg ggtcaaacag cagatcggca atttcacgtt taacgctgga
     6721 atggatggtg cagcgtttcc cggcaaaatc cctgttgcgg tataatccgc cgggaagaag
     6781 tcccatggca gcgtatcggg ttgccccatt aagaaagggg attgcttctg tattgataac
     6841 aatttcaagg gaactatcgc caagcatttc attgagatga cctatgagcc caaagccggt
     6901 tacatcggtc atggcatgga tatgttcagg gtatgggtag ccagccatgc aatcattgat
     6961 tgtagtcatg gagctaatac attcatcaat gtggttgctg tcagcaacgc cggcttttat
     7021 ggctgtattg atggttccgg tgccaagcgg cttggtaagg ataataacgt cgtctggctg
     7081 tatgccggta ttggtaatta tggcatcagg atgtataagc ccggttaccg ccagtccata
     7141 tttaagttca acatcatcaa tgctgtgacc gccaatgagc tgtatgccgg tttgctgtaa
     7201 cgtctgaagc ccgcccagta atattttttc cagcacatcc atggtaaatg ttgtaacggg
     7261 aaagcataca atatttaagg ctgtaatggg tgtgcctccc atagcgtaga tatcggatat
     7321 gctgttaacc gctgcaatct ttccaaagac aaaaggatta tccactatgg gagtgataat
     7381 atccacagtt tgaacaattg ctacatcatc actgatttta tacacgccgg catcgtcgcc
     7441 cgtctgcatg ccgacaagca cattgccggt ataggtgatg gggagctttt taactattgc
     7501 atcaaggtcc gccggaccta tctttgcagc tcaccctgac ccgcgtaccg attcagtaag
     7561 gcgcaacttc ttttttggat tgatgttaca gctttccatt gtattatcct tcatgtaaca
     7621 gtatatagta tatagcgcca gtgacaacat aaaaatgttt ggcccgaaga aaaatttgta
     7681 tcaataattt ttatgaaacg cacagtgctg tgtgtgtttc attgacacat actttttggg
     7741 cgatagttac tataaaacaa tgctttttcc caacattttt agtaagggtt tgttttctta
     7801 attttgtttt tggtttgtct tgaagtggta tgttatatat cacgccattg taataattcc
     7861 gaatgattca gtggtaattt tttgtttaac gttagcattt ttatataatg tataaaaata
     7921 tataaaagct gccgaaaggg tgatggaaat atttagcggc agtcatcctg aaatgtagaa
     7981 ggttatcctg aatcttgtgc agtcgatgca gaagtgtttt tttatgtatt gtta
//
LOCUS       DAOSOX010000105         8000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9008_length_8000_cov_8.037508, whole genome shotgun sequence.
ACCESSION   DAOSOX010000105 DAOSOX010000000
VERSION     DAOSOX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9008_length_8000_cov_8.037508"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLX22_08235"
     CDS             <1..212
                     /locus_tag="PLX22_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HOT19924.1"
                     /translation="MEFLWKTLNIEKRPLLTTYTVKNLGSRLQFSIDKAKRELGWKPK
                     VSFQEGFARTMDWLKTLDSVSLKTK"
     gene            237..1070
                     /locus_tag="PLX22_08240"
     CDS             237..1070
                     /locus_tag="PLX22_08240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT19925.1"
                     /translation="MENFSGKTVFITGGSSGIGLAVAKALSQEGANIAIFARNKMRLA
                     ESLITIEQVKRRHGQRFAWYQCDVSDYTQVKKTFTQALRQFDGCDILINCAGRAYPRY
                     FEDISIKQFEETMKINMFGVWNTCWFLVPHMKANGGYIVNTSSVAGLVGVFGYTDYSA
                     SKFAIIGFSEALKSELKRYNITVSVLCPPDTDTPGFAVENKTKPDETKEISKSAKLLT
                     PDEVAAALVQGMKKKKFIILANFDSKLTWFVKRYAPWLVELIMNSKITGIQKKKKKTK
                     K"
     gene            1105..1506
                     /locus_tag="PLX22_08245"
     CDS             1105..1506
                     /locus_tag="PLX22_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19926.1"
                     /translation="MSLKTAGVLSIVTGVGILLFWIAFFTVGLAPDIPPACYFSYEYA
                     FPLPDTILSLALIIGGIYALQRHAMKIILLPACGGLVFLGLLDMAFNYQNGMYTITVI
                     DGVLNAFINIWCVVFGLCAILTLQREYVKKS"
     gene            1567..1641
                     /locus_tag="PLX22_08250"
     tRNA            1567..1641
                     /locus_tag="PLX22_08250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1602..1604,aa:Val,seq:gac)
     gene            1667..3571
                     /gene="thrS"
                     /locus_tag="PLX22_08255"
     CDS             1667..3571
                     /gene="thrS"
                     /locus_tag="PLX22_08255"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT19927.1"
                     /translation="MATLQLDNGKTVEVPDGKTLLDVLPQLNSKAKPVAARINGLLKD
                     LRSPVTNDATVELISFDSPEGRKVFWHSASHLMAQAVKRLYPQVKVSIGPAIDNGFYY
                     DFDKPGGFSHEDLEKIEAMMHSIVNENIPIIRKELSRQEAIELFKASGELYKVELLQE
                     LSDNIVSLYEQGDFVDLCRGPHVPGTGYIKAFKLLSIAGAYWRGDEKNAMLQRIYGIA
                     FPDERMLKEHLDFLEEVKKRDHRSLGRDLDLFSIHEDAGGGLIYWHPNGGRLRATLEQ
                     WWRDEHFKHGYEILYTPHIGRATLWETSGHLDFYKENMYSPMDIDGNDYYIKPMNCPF
                     HIKIYETSLHSYRDLPLRWAELGTVYRYEKSGVLHGLLRVRGFTQDDAHIFCTPEQME
                     DEVREVLRFSLYMWKVLGFSDIKAYLATRPEKAVGDESRWKDAQESLLKAVKAEDLAV
                     EVDEGGGAFYGPKIDLKVKDAIGREWQMTTIQFDFNMPERFDMYYIGSDGQKHRPYMI
                     HRALLGSIERFIGILTEHYAGRFPVWLSPVQIILVNVGKQQVEYTNNLCAMLRSQGIR
                     AITDVRDETIKYKIRDAIEKKVPFIGVIGDREMQGNAIAVRKRGEQKAESMSIDAFVK
                     VLQQLSVDKQ"
     gene            3765..4313
                     /gene="infC"
                     /locus_tag="PLX22_08260"
     CDS             3765..4313
                     /gene="infC"
                     /locus_tag="PLX22_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT19928.1"
                     /translation="MDSKRFRVNEEIRASMVRLVGEEGGPQVIPVNEALQKAMGMGLD
                     LVEISPDQDPPVVKIVDYSKFKFEQIKKAKEAKKKQKVIQVKEIKLRPSIDLHDFQHK
                     VRHAREFIEEGNKVKFTVMFRGREMVHSDLGFKVLEEVKQALEDVAAVETNPSKEGRN
                     ITMIMAPMTAVQKKRVQEQNNQ"
     gene            4331..4528
                     /gene="rpmI"
                     /locus_tag="PLX22_08265"
     CDS             4331..4528
                     /gene="rpmI"
                     /locus_tag="PLX22_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004696338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT19929.1"
                     /translation="MPKLKTNSGAKKRFKVTATGKVKRAKGWKSHLLESKSPKRKRAL
                     RKAVCVSDADMKRFARLLPYS"
     gene            4551..4907
                     /gene="rplT"
                     /locus_tag="PLX22_08270"
     CDS             4551..4907
                     /gene="rplT"
                     /locus_tag="PLX22_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT19930.1"
                     /translation="MPRATTGKVHHKRREKILKDVKGFYGARKNLYRTAKDARRKKLQ
                     NSYEGRRLKKRDFRALWILRINAAARLCGISYSKLIGGLKKANILIDRKMLAELAIKD
                     LNAFKAIVDKVTKKAA"
     gene            4930..5847
                     /locus_tag="PLX22_08275"
     CDS             4930..5847
                     /locus_tag="PLX22_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19931.1"
                     /translation="MIQIKSAILMALFYVIFIHGNTIVSAPLTPSIYTVNEGMVEGSV
                     STDYVSAHSSFMKEKIVLNLGVMPKTNLRFTAGWLQIIDNNSLSSSTIGDSSIGIWHS
                     FGSLFNNFILGYYIDIGLATGQNAFNDETKYPTESGFSTVSWGLVGAYTVHNVTFIMA
                     GKYELMPEDSSSVFDGLTFNVADSNTWKRFFGLNPFSKDAMLYYKKFKNDIIAFSLTA
                     LYNGYPVILYMSLSSGNRLNNRPVYGDHNLPILYKGYNDTIFMVVASGARYFFNEKTY
                     FGAFLVYNIIRDYSYAVKYSLGIEVSSLW"
     gene            6060..6716
                     /locus_tag="PLX22_08280"
     CDS             6060..6716
                     /locus_tag="PLX22_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19932.1"
                     /translation="MITMQQLEELESRIIKALQLIGDLRAENAQLEANNESLKVQAEE
                     ARLSLEEKEQEIARLKKEIDKATNELSQIKQKEEVLEKKIVEMLGKLDTLKSGIPSYQ
                     AKPAPGTASTKAQPQQKPSVKAEPEPRAEEDFMEDSGGDEDIIIIDDTETAKTDIEVE
                     TLPTEEDEIVLLDDTDDEIIIDDVNDDLIIIDDDSAEKKSKDKTSDDDDFIIIEDEEK
                     "
     gene            6713..7054
                     /locus_tag="PLX22_08285"
     CDS             6713..7054
                     /locus_tag="PLX22_08285"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOT19933.1"
                     /translation="MISSANNPNKVKVTILGQVYTIEGDASCDHIIKVADYVNQKMED
                     VANTISHANSLQIAILAALNIADELFQIKESEYMMSDEIKEKTNMLISMLDEGLIGDI
                     FSNVSKKSQHL"
     gene            7135..7704
                     /locus_tag="PLX22_08290"
     CDS             7135..7704
                     /locus_tag="PLX22_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT19934.1"
                     /translation="MDQSIKALSDEELVQSVMHGNDRAFEELYERYSERIFKLLYSYV
                     YNVDDAVDLMHDVFIRAYRHLNKFDVRRAFSAWLYTIAINCAKNYRCKVYKNDTMIER
                     EEQRIMNTDAMKTPEDHVIENELLQEFSKAVDALNNKFREVFLLRYGQQLQYSDIAHI
                     LDISERTAKWRMERALAFITDHLKKKGVI"
     gene            7760..>8000
                     /locus_tag="PLX22_08295"
     CDS             7760..>8000
                     /locus_tag="PLX22_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19935.1"
                     /translation="MNRQIDDNELLHEIAPYAGKASDVLLKIIRQWQSSLPALEDTLT
                     SNDIIEKAKTDSQGIHIKKLVAMFATGIAAIVIAGV"
BASE COUNT         2592 a         1246 c         1852 g         2310 t
ORIGIN      
        1 ttatggaatt tttatggaaa acattgaaca ttgaaaagag gcctcttttg acgacctaca
       61 cagtaaaaaa tttgggttct cgcttacaat ttagcattga taaggcaaaa cgagaactgg
      121 ggtggaagcc aaaagtttca tttcaggaag gctttgcacg aacaatggat tggttgaaaa
      181 cccttgatag tgtgtcatta aaaacaaaat aatgtaattg catggtgagg ttaattatgg
      241 aaaatttttc tggaaaaaca gtgtttatta ccggtgggtc aagcgggata gggttggcgg
      301 tggcaaaggc gttgtcgcag gagggtgcca atattgccat atttgcccgt aataagatgc
      361 gactggctga atcattgata actatcgaac aggtaaaaag acgccatgga cagcgttttg
      421 catggtacca gtgtgatgtt tcagattata cgcaggttaa aaagactttt acccaggctt
      481 tgcgtcagtt tgatgggtgt gatatcctta tcaactgtgc aggcagggca tatccacgat
      541 attttgagga tatctccatc aagcaatttg aagagaccat gaagattaat atgtttggtg
      601 tgtggaatac ctgctggttt ttggttccac acatgaaggc aaatggaggg tacattgtta
      661 acacatcatc agtggcaggg cttgtaggag tttttggata taccgattac agcgcctcaa
      721 aatttgccat cataggattt tcagaggctc tgaaaagcga gcttaaacgg tataacatca
      781 cggtatcggt gctatgcccc cctgatacgg atacccctgg ttttgctgtt gaaaacaaaa
      841 caaaaccgga tgaaacaaaa gagatatcca aatcagctaa attgttaaca ccggatgagg
      901 ttgcggctgc actggtgcag ggaatgaaga aaaaaaagtt tattatcctg gcaaattttg
      961 acagcaagtt gacatggttt gtaaaacgat atgcgccctg gcttgttgag ctgattatga
     1021 acagcaagat aacgggcatt caaaagaaaa aaaagaagac aaaaaaatag tacaataata
     1081 aaccataata aaaggaggaa cgatatgtca ttgaaaactg ctggtgtttt atctattgta
     1141 acaggtgttg gtatcctgtt attctggatt gcttttttta ctgttgggct ggcaccggat
     1201 atcccgcctg catgttattt ttcatatgaa tatgcattcc cattaccgga taccatattg
     1261 tcgctggcat tgataattgg cggcatatat gccttacaac gacatgcaat gaagattata
     1321 ctgttgccgg cctgtggcgg gcttgtcttt ttggggttac ttgacatggc tttcaactat
     1381 caaaatggca tgtacaccat cacggttata gatggcgtgt taaacgcttt tatcaatatt
     1441 tggtgtgttg tttttggtct atgtgctata ttgactttac agcgggaata tgtgaaaaag
     1501 tcttgacagt ttattagcac ttttttaact aaagattcat ttatagtttt ttttggcgat
     1561 agttatgggc gattagctca gcctggttag agtgcctgct cgacatgcag gaggtcactg
     1621 gttcaactcc agtatcgccc aatttatttt aggattggca aaaataatgg caactttgca
     1681 attagacaac gggaagacag ttgaggtgcc tgacgggaag acactgcttg atgtgcttcc
     1741 acaattaaac agcaaagcaa aaccggttgc ggcacgtatc aatgggcttt taaaagattt
     1801 gcgctcacca gttaccaatg atgcaaccgt agagcttata tcatttgata gtcctgaagg
     1861 gcgtaaagtt ttctggcatt ctgcatcgca ccttatggca caggcggtaa agaggctata
     1921 tccccaggtg aaggtatcga ttggtccggc tattgacaat ggtttctatt acgattttga
     1981 taaacccggt ggtttttctc atgaggattt agagaagatt gaagcaatga tgcactccat
     2041 agtcaatgag aatatcccga ttatacgaaa ggaattatcc cggcaggagg caatagaact
     2101 gtttaaggcg tcaggtgaac tatataaagt tgagctgcta caggaactat cggacaacat
     2161 tgtgtcgttg tatgaacagg gggattttgt tgatttatgc cgtggaccac atgtacccgg
     2221 taccggatat atcaaagcat ttaagctttt gagtattgcc ggcgcgtact ggcgcggtga
     2281 cgaaaaaaat gcaatgcttc agcgtattta tggcattgcg ttcccggatg aaagaatgct
     2341 gaaagaacat cttgattttt tagaagaggt aaaaaaacgg gatcatcgta gtttaggcag
     2401 ggatttagac ctgttcagca ttcacgaaga tgctggcggc ggtttgatat actggcatcc
     2461 caatggcgga aggcttagag caacgctgga gcaatggtgg cgtgatgagc actttaaaca
     2521 tggatacgaa attttgtata ctccgcatat agggcgtgcc acactatggg aaacatcagg
     2581 gcatctggac ttttataaag aaaatatgta ttcgccaatg gatattgatg gcaatgatta
     2641 ctatataaaa ccaatgaatt gcccattcca tataaagata tatgaaacaa gcttgcattc
     2701 gtaccgtgat ttgccgctac gatgggctga gcttgggacc gtataccgct atgaaaaatc
     2761 cggtgtattg catgggttgt tgcgggtgcg tgggtttaca caggatgatg cacatatatt
     2821 ctgtactcct gagcaaatgg aagatgaggt gagggaggtt ttgcgttttt cactgtatat
     2881 gtggaaggtg ttgggatttt cagacataaa ggcatatctg gctaccaggc cggaaaaagc
     2941 agtaggggat gaatcacgat ggaaggatgc acaggaatca ttgctgaaag cagtgaaggc
     3001 tgaagattta gctgttgaag ttgacgaagg tggcggtgca ttttatggtc ccaaaattga
     3061 tttaaaggta aaggatgcaa ttggtaggga atggcagatg accaccattc agtttgattt
     3121 taatatgccc gaacggtttg atatgtatta tattggaagt gatggtcaga agcaccggcc
     3181 ctatatgata cacagagctt tgcttgggtc aattgagcga tttattggta ttttaaccga
     3241 acattatgca gggcgtttcc ctgtgtggtt gtcacccgta cagataatcc ttgtaaatgt
     3301 tggcaagcag caggtagaat ataccaataa cctgtgcgca atgttgcggt cacagggtat
     3361 cagggctata accgatgtgc gcgatgaaac gatcaaatat aaaatacgtg atgcaataga
     3421 aaaaaaggta ccgtttatag gggtcattgg tgacagggag atgcagggaa atgcaatagc
     3481 agtacggaag cggggagagc agaaggctga atccatgtcg atagatgctt ttgtaaaggt
     3541 tttacagcaa ttgtctgttg ataaacagta attatgatat ttttacctga cactattgca
     3601 actgaggcat ataatacttt ttttatgttt gttatggaat tattgttgat taatatatat
     3661 cagtgatatg atgtggaaga tttttttgtt gctatatttt ttaaagtaat tttgtatatg
     3721 gtgtataatt ttattgtatt ggtaaggagg agaacataaa ccgtattgat agtaaacgtt
     3781 tcagggtaaa tgaggagata cgagcctcca tggttcgttt agttggcgaa gaaggtggtc
     3841 cacaggttat tccggtaaat gaagcattgc aaaaagcaat ggggatggga ttagatcttg
     3901 tagaaatatc ccctgatcag gacccgccgg ttgtgaagat tgttgattat agcaaattca
     3961 agtttgagca aatcaaaaaa gctaaagaag ccaagaaaaa acagaaggtt attcaggtaa
     4021 aagagattaa actaaggcct tctatcgatt tgcatgattt tcagcacaaa gtgcgccacg
     4081 cacgcgagtt cattgaagaa ggcaataaag taaaatttac tgtcatgttc agagggcgtg
     4141 agatggtgca tagtgattta ggttttaagg tgttagagga agtgaaacag gcgttggaag
     4201 atgtcgctgc tgttgaaact aatccttcaa aagagggtcg aaatataacc atgataatgg
     4261 ctcccatgac agctgtacaa aaaaagcggg ttcaggagca aaataatcaa taagtgaaga
     4321 gaggtgcatg atgcctaaac taaaaactaa tagtggagca aagaaacgat ttaaggtgac
     4381 agctactgga aaggtaaaac gagcaaaagg ctggaaaagc catcttttag aatcaaaatc
     4441 accaaagcgc aagcgtgcat tacgaaaagc tgtgtgtgtc agtgatgctg acatgaagcg
     4501 gtttgcacga ttgctgccat attcttaatg aaacatgaga ggtaataatt atgccacgag
     4561 caacaacagg taaagttcat cataaaagac gtgaaaagat attaaaggac gtaaaaggtt
     4621 tctacggtgc acgtaagaac ctttacagaa ctgcaaaaga tgcacgaagg aaaaaattgc
     4681 aaaattcgta cgagggacgg aggcttaaaa agcgtgattt cagagcgtta tggatactac
     4741 gcatcaatgc tgctgcacgg ctttgcggta tttcgtatag caagcttatt ggcggattaa
     4801 aaaaagctaa tatacttatt gacagaaaga tgctggcaga attggcaata aaagatctta
     4861 atgcgtttaa ggcaatagta gataaagtaa cgaaaaaggc tgcttaaaca ttacggtact
     4921 atgaaccgaa tgatacaaat taaaagcgcc atcctgatgg cgctttttta tgttattttc
     4981 attcatggca ataccattgt tagtgcccct ttaacaccct ctatctatac agttaatgaa
     5041 ggaatggttg agggctctgt ttctacagat tatgtcagtg cacattcatc attcatgaaa
     5101 gaaaaaatag tgcttaacct gggtgttatg cctaaaacaa atcttcgttt taccgctggc
     5161 tggctacaga ttattgataa taatagttta tcaagcagta ccataggtga ttcatccatt
     5221 gggatatggc atagctttgg ttcgttgttt aacaacttta ttctgggata ttatatcgat
     5281 ataggattgg caacgggaca gaatgccttt aatgatgaaa ccaaatatcc aacggaatcc
     5341 gggttttcta ctgtatcatg gggattggtt ggtgcatata cggtacataa cgtaacattt
     5401 attatggcag gtaaatatga attgatgccg gaagatagtt catcagtttt tgacggcttg
     5461 acatttaatg tggctgacag caatacatgg aagagatttt ttggattaaa tccttttagt
     5521 aaggatgcca tgctgtatta caagaagttt aaaaacgata tcattgcgtt ttcattgact
     5581 gcactatata atggttaccc tgtaatactg tatatgtcat tgagctctgg caataggctt
     5641 aataatagac ccgtttatgg tgatcataac ctccccattc tttataaggg gtataacgat
     5701 actattttta tggtggtagc gtcaggtgca cgttattttt ttaatgaaaa aacttacttc
     5761 ggagcctttt tggtgtacaa tattatccgt gattatagct atgctgtaaa atattcactg
     5821 gggattgagg tttcttcttt gtggtgaaat ttttgtaatt acttaaaaaa ctataggaaa
     5881 aactcggaaa gcaaatgaat tgtataaata attaaaaaaa ctcttgattt tatatattgg
     5941 tcattatact ttattgttat tgatgatgtc attttaaatt ttagtttact aaaattttat
     6001 tttcaccgac atttaaagca gtgatacatg gaaaaatgat aattcgggag gatgttctta
     6061 tgattacaat gcagcaactg gaagagctcg aaagcagaat tataaaagca ttacagctta
     6121 taggagatct aagagctgag aatgcacaat tagaagctaa taatgaatct cttaaagtgc
     6181 aggctgaaga ggcaaggctt agtctagaag aaaaagaaca ggaaatagca aggcttaaaa
     6241 aggagattga taaagctact aatgagctgt ctcaaattaa gcaaaaggaa gaggttctgg
     6301 aaaagaagat tgttgaaatg ttaggtaaac ttgatacgct aaaatccggg attccttcgt
     6361 atcaggcaaa acctgcgcca ggcacagctt caactaaagc acaaccacag caaaagccat
     6421 ctgtaaaggc tgagccagaa cccagggctg aagaagactt tatggaagac agcggcggtg
     6481 atgaggatat tatcattatt gatgatactg agactgcaaa aactgatata gaagttgaaa
     6541 cattgccaac tgaagaagat gagatagtcc tgttagatga tactgatgat gagataatta
     6601 ttgatgatgt taatgacgat ttgataatca ttgatgatga ttcagcagaa aaaaagtcta
     6661 aagataagac ttccgatgat gacgatttta tcattattga agatgaggaa aaatgatatc
     6721 ttctgcaaat aatcctaata aagtcaaagt gaccatatta ggacaggttt acaccattga
     6781 aggagatgca tcatgcgacc atattataaa ggttgccgat tatgttaacc agaaaatgga
     6841 agatgtagct aacactatat cacatgcaaa ctctttgcag attgctattt tggcagcatt
     6901 gaatattgct gatgaattat ttcagattaa agaatcagag tatatgatga gcgatgaaat
     6961 aaaagaaaaa accaatatgc tcatctcaat gctggacgaa gggcttattg gtgatatttt
     7021 tagcaatgta agcaaaaaaa gccagcatct ataagagatt gatatgaaaa gatataaaaa
     7081 aaataaaacc cgctttgcac ttttatccta tcccggtgat atattataat aacaatggac
     7141 caatccataa aagctctatc tgatgaagaa ttggtacaat cggtaatgca tggcaatgac
     7201 cgtgcatttg aagagcttta tgagcgctat tcggagcgaa tttttaaact tttgtacagc
     7261 tatgtttata atgtagatga tgcggttgat ttgatgcatg atgtgtttat acgggcatac
     7321 aggcatttga ataaatttga tgttagaagg gctttttccg catggttata taccatagca
     7381 ataaattgtg ccaaaaacta tcgctgtaag gtatataaaa atgataccat gattgagcgc
     7441 gaggagcagc gtataatgaa tactgatgcc atgaaaacgc ctgaagatca tgttattgaa
     7501 aatgaattgt tgcaagagtt ttcaaaggcg gtagatgcat tgaataataa attcagagag
     7561 gtatttttat tacgatatgg gcagcaattg caatatagcg atatcgcaca tattctggat
     7621 atttcagaac gcactgcaaa atggcgtatg gaaagggcgc ttgcctttat tactgatcat
     7681 ttaaagaaaa aaggagtcat atgaaatagt atatgataga tagttttttg aaacatacag
     7741 caaaacacat gggattgtta tgaatagaca aattgatgac aacgaattgc tacatgagat
     7801 tgcgccgtat gcaggaaaag catcggatgt tttgctaaaa ataatccggc aatggcagtc
     7861 ttcattgccg gcacttgagg atacacttac ttcaaacgac atcattgaaa aagcaaaaac
     7921 tgattctcag ggtatacaca taaaaaagct tgttgctatg tttgcaactg gtatagcggc
     7981 gatagttatt gctggagtaa
//
LOCUS       DAOSOX010000106         7926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9119_length_7926_cov_8.796468, whole genome shotgun sequence.
ACCESSION   DAOSOX010000106 DAOSOX010000000
VERSION     DAOSOX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9119_length_7926_cov_8.796468"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..661
                     /locus_tag="PLX22_08300"
     CDS             320..661
                     /locus_tag="PLX22_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT19936.1"
                     /translation="MDITKRTRGDVVILDISGEIDLYNAPEIKDIINGLIEEKKFNVI
                     INLEKVTYIDSSGIGALISSLSNLKKYQGGLKIINVFASVRKVFELTKLTSFFEIYDS
                     EEVAIESFNKR"
     gene            919..2244
                     /locus_tag="PLX22_08305"
     CDS             919..2244
                     /locus_tag="PLX22_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOT19937.1"
                     /translation="MRSQYHISVIALLSALVLMTACFEKLPVKELSLAKNAISDAEAV
                     KAPYYAKDEYNQAKDLLVAAHDNVKNEEYDKAQQNAIEAQKKAREAYDKAIPLLAKDT
                     LDIATKSVEAADNVYAGVLAKDDFAKANEMLQEANKNYENKNYLATYKAALDADMYAK
                     NARNVALSQKETLADSIKEVNIIIAQAESYNANQYAPEKLKLAKEQSQVARSAYDAEE
                     LKKGFSAIEVAKLNADEAYFASLKATAAKKIDEAQGAIVAAKATEQAKQSPDDIKAAE
                     ESLSNAKTLFNDAKYKESIIASGEAITIANSVSAKKVAVAPVIESEVTPEGKKEVQSE
                     TQKEALQKKVEEETEYTMYKVKYNPEKRDCLWRIAVKFYNDGFKWKLIYEANKDLVKD
                     PNLIKPGWVLKIPKTATKLAQPKKEGVNEQTAVKEDEAPTTELKMKTQE"
     gene            complement(2283..3059)
                     /gene="mazG"
                     /locus_tag="PLX22_08310"
     CDS             complement(2283..3059)
                     /gene="mazG"
                     /locus_tag="PLX22_08310"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT19938.1"
                     /translation="MSQSPLYHLKEIAGILRSEHGCAWDKAQTSDTLKSYLIEEAYEV
                     YQAIEHGDIDNLKEELGDLLYQVYAHAQIASEHDLFDIDDIANSIIQKLIRRHPHVFG
                     NETANDKYEVINRWEKIKKLEKNSGQSLLDGVPYHLPALLKAYRVQQKVARVGFDWEK
                     LHDIIAKLNEEVNEFAVELQKNKENKEALKDEIGDIFFTIVNIARHLEINPEEALQHT
                     IAKFTKRFHYIEQRLNEMNKDIAQTPLSELDALWDESKQK"
     gene            3304..4566
                     /gene="glyA"
                     /locus_tag="PLX22_08315"
     CDS             3304..4566
                     /gene="glyA"
                     /locus_tag="PLX22_08315"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT19939.1"
                     /translation="MALHKFLKDADPEVFSILQKENMRQENSLILIASENYTSQAVLE
                     ASSCTMTNKYAEGYPGKRYYNGCQFVDEMERLAIERIKKIFNADVANVQPHSGTQANM
                     AVLMALLEPGNTIMGMNLSHGGHLSHGSPVNFSGIYFKVVSYGVTKDTQRINYDEAYS
                     VAKEHKPKIIIAGASAYPRTIDWNKFREIADEVGAVLLTDIAHIAGLVATGHHPSPVP
                     VADFVTMTTHKTLRGPRGGIILTKQKYEGMMNKYIFPGIQGGPLMNTIAAKAVAFGEA
                     LQPSFKDYSAKVIANAQALARTLLSRGLHLVSGGTDNHLMLVDLHNKNLTGKDVANRL
                     DEAGITCNKNGVPFDDKSPMITSGIRLGSPALTTRGLNEDDFKHIGNLICDIIDNMND
                     DKVLQRVQAQVQELCKHYPTDFLRLEPR"
     gene            4636..5625
                     /locus_tag="PLX22_08320"
     CDS             4636..5625
                     /locus_tag="PLX22_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT19940.1"
                     /translation="MKRLIGVIMICLIAVVGCKKEKKYTVAREGLVNLISGDVVLVVN
                     GAESKAQIGDAIKEGMIIKTVGPKSIAEIYFGENAVKILGDTVIKIEKLITDISANTE
                     ASSFVVEKGRMFSRVTKKLNKGDSYQVKTPTAIAAVRGTDFLVTQEEDKSNVACLDGL
                     LEVLNQSLATGQPIVLEEKQEVDIVPGQDMVKKQISEDKLRMLNILLEIQAMREDIRK
                     KYEQQKEEILKYVEEQKEANKTMLEEQREKDKALVEEQKARDKAMIEGIKGQIKDESG
                     KAAQQSLDAAKSQMQDAKAVDKDQASSEAKDQMSAMKPKIEKQKIDLNQFKTQ"
     gene            complement(5961..6431)
                     /locus_tag="PLX22_08325"
     CDS             complement(5961..6431)
                     /locus_tag="PLX22_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOT19941.1"
                     /translation="MKRIHFISLLLIVSLSGCGLEDLLHKIAFKLSYYMAKSKLEKQF
                     EEEQGIILAPPPSLSKFDIAPIDTKTNDKKPHFLRITMSLGYEENEDLESELKQRKDA
                     INHICRILIKDKSSEEVNSVEDTVNLAEEIKAHINSLLTKGKIKEVYFKEFVVK"
     gene            6761..7546
                     /locus_tag="PLX22_08330"
     CDS             6761..7546
                     /locus_tag="PLX22_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19942.1"
                     /translation="MIQLAFIGTGTCNATARKPQALAFSIDGELVCVDFGGGAYHAIA
                     SLNNPDFNYGNISTVVLTHYHVDHISGLPDLLWGEMWDYTGHRTEPLTFVGPKGLKNF
                     YEDRLLPFMGDYPLPFKVELVELHDGDIYKGNGFTLQSNTLAHGDFSSGYLFDCGSKK
                     IAITGDTGYCDALQRLLSVADMAVIEWGIASDDTYPLHVSSKDIISLVNAGIWPQQVY
                     AVHVYPLPDLDFETQLQTMRSVVASHNKSIEFPSDGDIITIIP"
     gene            7655..>7926
                     /locus_tag="PLX22_08335"
     CDS             7655..>7926
                     /locus_tag="PLX22_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT19943.1"
                     /translation="MKAIKVDYCIIGAGVVGLAIARELSLRTKKTIVVCEKEEQAGLH
                     ASGRNSGVLHAGIYYTQGSLKANTCIEGNRLMKEFCAEKGVAVKESG"
BASE COUNT         2662 a         1279 c         1619 g         2366 t
ORIGIN      
        1 ttatgaaata ttttatggac actatacaga aaagcatagc aaacaatcaa tttatggatg
       61 aatataacaa atggagaaaa atatataaca acagtgaaag aaataatgag taagcaactg
      121 tatggttgac gttgatctga ctatttcaat gaatatttca atggtgtaat ttaaacttga
      181 acgtacgtta tttattgtgt ataaataaaa aaaattattt acaaaacaaa aaaaataaaa
      241 tatcttgaca ttttgctttt gttattatag tatttagttt caataccatg caagcattac
      301 ttttgggagt agaatgtcta tggatattac gaaaagaacg agaggtgacg ttgttatact
      361 tgatatttct ggtgagattg atctttacaa cgccccggaa attaaagata taataaatgg
      421 gctcattgaa gaaaaaaaat ttaatgtaat cattaacctt gaaaaagtaa catatattga
      481 ttcgtcgggt attggtgctt taatttcaag cttatcaaat ctaaaaaaat atcagggtgg
      541 tttaaaaata attaatgttt ttgcttcggt tcggaaagtt tttgaactga ctaaacttac
      601 gtcgtttttt gaaatatatg attctgaaga agtagctatt gaatcattta ataaacggta
      661 atgccttgca aagtaaatgg tcgtgtattc aaaaaaatta cccatggccg gcatgacaat
      721 gtgttggcat gtcattctga acggagtgaa gaatctcgtc gttgtataaa agaatagatt
      781 cttcgggact gcgtccctca gagtgacaat gctgggaagg tcatcctgaa ccatatttgc
      841 atcactttgt attcattaat tgataagcat ttgattaata aagagtatta tacattagaa
      901 aaatgaaaga ggtgtactat gagatcacaa tatcatatca gtgttattgc gctattgtca
      961 gctcttgtgc tgatgactgc ctgctttgaa aagttgccag ttaaggagct atcgcttgca
     1021 aaaaatgcta tcagtgatgc tgaagcagtg aaagccccct actatgccaa agatgaatac
     1081 aatcaggcaa aagacttgct tgtagcagcc cacgataatg taaaaaatga agagtatgat
     1141 aaggcacagc aaaatgcaat tgaagcacaa aagaaagcca gagaagcata tgataaagct
     1201 attccattgc ttgccaaaga tactcttgat atagctacca agagcgttga agctgctgat
     1261 aatgtgtatg caggtgttct tgcaaaggat gattttgcca aagccaatga aatgttgcag
     1321 gaagcaaaca aaaactatga aaataaaaat tatttagcta cctacaaagc tgctctggat
     1381 gcagatatgt atgccaagaa tgcacgcaat gttgcattga gccagaaaga aactttagct
     1441 gattcaatca aggaagtcaa cattattata gcacaggctg agagctataa tgctaaccag
     1501 tatgctcctg aaaaattaaa acttgcaaag gagcagtccc aggtagcgcg tagtgcttat
     1561 gatgcagaag agctcaagaa aggtttttca gccattgaag ttgccaagct caatgccgat
     1621 gaagcatatt ttgcatcatt aaaggctact gcagctaaga aaattgatga agcacaggga
     1681 gcaatagtag cagcaaaagc taccgaacag gcaaaacaat ctccagacga tattaaagca
     1741 gcagaagaat cgctttctaa tgcaaaaact ttatttaatg atgcaaaata taaagaatca
     1801 ataattgcct ccggtgaggc aattacaatt gcaaattcag tatcagcaaa aaaagtagcc
     1861 gtggctccgg tcattgaaag cgaggtaaca ccggaaggta aaaaagaagt gcaatcagaa
     1921 actcagaagg aagcattgca gaaaaaagta gaagaagaaa ccgaatacac catgtataag
     1981 gtaaaatata atcccgaaaa aagagattgt ttatggcgga tagctgtaaa attttacaat
     2041 gatggattta aatggaaatt aatatatgaa gcaaataaag atttagtaaa agatcccaat
     2101 cttattaagc caggctgggt tttgaagata cccaaaactg ccacaaagct tgcacagccg
     2161 aaaaaggaag gcgttaacga gcagacagca gttaaggaag atgaggcacc aactactgaa
     2221 ttgaagatga aaactcagga ataaattaaa gaaaggggtg tttgcgacac ccctttttaa
     2281 tgtcattttt gttttgattc atcccacaat gcatccagtt cacttaacgg tgtttgagca
     2341 atatctttgt tcatttcatt taatcgttgt tcaatatagt ggaatcgttt tgtaaacttt
     2401 gcaatagtgt gttgtaaagc ttcttcaggg tttatttcca gatggcgtgc aatgttaaca
     2461 atggtgaaaa aaatatcgcc aatttcatct tttaatgctt ctttattttc tttatttttt
     2521 tgcaattcta cggcaaactc attcacttct tcattcagtt ttgctataat atcatgtaac
     2581 ttttcccaat caaagccaac gcgcgctacc ttctgctgaa cgcgatatgc ttttagtaaa
     2641 gctggtagat ggtagggaac gccatcgagc agtgactgac cgctattttt ttccagcttt
     2701 tttatctttt cccagcggtt tataacttca tatttatcgt ttgcagtttc attcccaaat
     2761 acatgaggat gacggcggat taatttttga atgatactat tcgcaatatc atcaatatca
     2821 aataaatcat gttcgctggc aatctgtgca tgagcataaa cctggtataa caaatccccc
     2881 agttcctctt taagattatc aatatctcca tgttcaatgg cctgatatac ctcataggct
     2941 tcttcaataa gataagattt gagtgtgtca gaggtctgtg ccttatccca tgcgcatcca
     3001 tgttcactgc gtaatatgcc tgcaatttct tttaaatgat ataatggtga ttgtgacatg
     3061 attgtaatcc ttcataataa tttttttaac ttttctatac cgatactgat actgatcatc
     3121 atacattgtg acaatagtct acttataaaa aatgtattga ccactatttt agacatagta
     3181 taccacacca aaaaaagaaa aattttttta ggttgacatc attttataat tgtgaacata
     3241 cgcactatgt ggcatacatg attatgtaaa tatagattac aattctatca atgaggtaca
     3301 ttcatggcat tgcataaatt tttaaaggat gctgaccctg aagtattttc tatcttgcaa
     3361 aaggaaaaca tgcgtcagga aaattctttg atacttatag catcagaaaa ttatacgtca
     3421 caagcagtac tggaagcatc atcctgcaca atgaccaata aatatgcgga aggatatccc
     3481 ggaaagcgct attacaatgg ctgtcagttt gtcgatgaga tggaacgttt agctattgaa
     3541 cgaataaaaa aaatttttaa cgcagatgtt gccaatgtgc agccacacag tggtacgcag
     3601 gcaaacatgg ctgtgctcat ggcactgctt gagccgggca ataccataat gggtatgaac
     3661 ctatcacatg gcgggcacct atcgcatggt tcgcctgtta atttttcggg tatatacttt
     3721 aaagtggttt cttatggcgt tacaaaggat actcaacgta ttaactatga tgaggcatac
     3781 agcgttgcaa aagaacataa gccaaaaata atcattgcag gagcatcggc ttatccacgc
     3841 accattgact ggaataagtt cagggaaata gctgatgaag taggcgcagt gctgttaaca
     3901 gatattgctc atatagcagg gttggttgcc acggggcatc atccatcgcc agtgcctgtt
     3961 gcagactttg ttaccatgac cacgcataaa accctgcgag gaccgcgcgg tggaatcatc
     4021 ctgacaaagc aaaagtatga aggcatgatg aacaaatata tcttccctgg catacaggga
     4081 ggacctttaa tgaatactat cgccgcaaaa gctgttgcat ttggtgaagc actgcagcct
     4141 tcatttaagg attattcagc aaaggttatt gcaaatgcac aggcgctggc ccggacattg
     4201 ctatcacgag gtctccatct ggtatcagga ggtaccgata atcatctcat gctggtagac
     4261 ctccataaca aaaatttgac agggaaggat gtggctaacc ggctggatga agcgggaata
     4321 acctgtaata aaaatggtgt gccgtttgat gataagtccc ctatgattac cagtggtatt
     4381 agattgggga gcccggcatt aacaacacga gggcttaatg aagatgattt taagcacatt
     4441 ggaaatctca tttgcgatat tattgacaat atgaatgatg acaaagtatt gcagcgtgta
     4501 caagcgcagg tgcaggagtt atgcaaacac tatcctactg acttcttacg tctggagcca
     4561 cgctaaatat agttcttgac tataataaga acactgtgta ttacaccagt atataaagta
     4621 atcaggagga acgttatgaa aagattaatt ggcgttatta tgatatgttt gatagctgtt
     4681 gtgggatgta aaaaagaaaa gaaatatact gttgcgcggg aaggtctggt aaatttaatt
     4741 tcaggcgatg ttgttctggt ggtgaatggt gctgagagca aagctcaaat aggtgatgct
     4801 attaaagaag ggatgatcat taaaactgtt ggtccaaagt caattgccga gatatatttt
     4861 ggcgaaaatg cagtgaagat tttaggtgac acggttatca agattgaaaa attgattacc
     4921 gatattagcg caaataccga agcatcatcg tttgtagttg aaaaagggcg tatgttctca
     4981 cgggtaacta aaaaattaaa taagggcgat agttaccagg taaaaacacc aactgcgata
     5041 gcagctgtgc gcggtacaga ttttctggta acacaggaag aagataagag taatgtagca
     5101 tgtctggatg gactacttga agtattaaac cagagcttag ctacaggaca acctatagta
     5161 cttgaggaaa aacaggaagt tgacattgta cctggtcagg atatggtgaa aaaacaaata
     5221 tccgaggata aactcaggat gttgaacata ctgctggaga tacaggcaat gcgtgaggat
     5281 atccgtaaga agtatgaaca gcaaaaagaa gaaatattaa aatatgtgga agagcagaag
     5341 gaagccaata aaacaatgct ggaagagcag cgtgaaaagg ataaagcgct tgttgaggaa
     5401 cagaaagcac gtgacaaagc tatgattgaa ggcataaaag gccagattaa agacgaatca
     5461 gggaaggcag cacagcagtc actggatgca gcaaagagtc agatgcagga tgcaaaggct
     5521 gttgacaagg atcaggcaag ctctgaagct aaagatcaga tgagtgccat gaagccaaag
     5581 attgaaaagc aaaagattga tttgaatcag tttaagactc agtaacagaa aaataattat
     5641 tgtaaaatca attatataat catcagggct gttacacagt atagtaacag cctttctatt
     5701 gttaatggta gtatatttta gtatgtttaa taatagtact attttcctgt ttatgagggg
     5761 tttgtagaaa ttttaacagg caatgttcta attccgggat tatggcatga acaattaatg
     5821 aagtgctttt aatagtaaaa tgtaatctca taaaatcaca atgaattaat ttcacgccgg
     5881 gggaattttt taatattttg aagcccttaa aattatgatg tttttgaata gctttaaaaa
     5941 gaaaaaaaat actgaaataa ttatttaaca acaaattcct tgaaataaac ttctttaatc
     6001 ttcccttttg ttaaaaggga atttatgtga gcctttattt cctctgctaa atttactgta
     6061 tcctctacgg aatttacttc ttcagaagat ttgtctttaa ttagtatcct gcatatgtga
     6121 tttattgcat cttttctttg ttttaattct gattctaaat cttcattttc ttcatatcct
     6181 aagctcatgg ttatccttaa aaaatgtggt tttttatcgt tcgtttttgt atctattgga
     6241 gcaatatcaa attttgatag ggagggtggt ggagcaagta ttatgccctg ttcttcttca
     6301 aattgctttt ccagttttga tttagccata taataactta atttaaatgc tattttgtgc
     6361 agtaaatctt ccagtccaca tcccgaaagt gatactatta ataatagtga aataaaatgt
     6421 attcttttca taattatttc ctcattttag tataaggata tttaatggat tacagattga
     6481 aaaataaaag tcaagtggtt ttcaaaaaaa agatatagtg caggttatcg tttttgatta
     6541 tattttggct aactgcaaaa gtataacttt aaatgaaaaa ttgttgtaaa gaccttacaa
     6601 ggcatatttt ggttttttga taatattgtt ttgaaaagtt atttccccaa atactaaagt
     6661 tatgtataaa tattgttatt gactaatttg cttatatgct ggaaactttt gcacagcatt
     6721 ggtactattt tataatacta ttgaatgatg gcattacgtt atgatacaat tagcgttcat
     6781 aggtacgggg acatgtaatg ccacggctag gaagccgcag gcgctggcat tttccattga
     6841 tggggagctg gtgtgtgttg attttggcgg aggagcttac catgccatag cttcattaaa
     6901 taatccggat tttaactatg gcaatatatc aaccgttgta ttaacccatt accatgtgga
     6961 tcacatatcg ggtttgcctg atctactctg gggtgagatg tgggactata ccggacaccg
     7021 cacagaaccg ttaacgtttg taggaccaaa agggttgaag aatttttatg aagacagact
     7081 gctacctttt atgggggatt atccactacc gtttaaagta gagcttgttg aattgcatga
     7141 tggcgatatt tataaaggca atggttttac gctccaatca aatactcttg cccatgggga
     7201 tttttcgtca ggctaccttt ttgattgtgg atcaaaaaaa atagcaataa caggtgatac
     7261 cggatactgt gacgcattac aacgattact ttccgttgct gatatggcag tcattgaatg
     7321 gggaatagcg tctgatgata cctatcctct ccatgtatcg tcaaaggata ttatatcctt
     7381 agtcaatgca gggatatggc cacagcaggt gtatgctgta catgtatacc cattgccaga
     7441 tcttgatttt gaaacacagt tacaaacaat gcgctctgtg gttgcttcac ataataaatc
     7501 cattgaattt ccttccgatg gcgatataat aacaattatt ccataagcta ttgcaataat
     7561 ttttaggtag cacacatgct taatcatgat gttcgttaag ctgtatatag tactttatta
     7621 ttacgattaa ttgggaattg cgaaaagcac atttatgaaa gctataaaag tagactattg
     7681 tatcattggt gcaggtgttg ttggattggc aatagcacga gagctatcgc tacgcactaa
     7741 aaaaacaata gtagtatgcg aaaaggagga acaggctggt ttacacgcat caggaagaaa
     7801 tagtggtgtg ctgcatgctg gaatttacta cacacagggg agtttaaaag ccaatacatg
     7861 tattgaaggc aacaggctta tgaaagagtt ctgcgctgaa aaaggggtag cagttaaaga
     7921 atcagg
//
LOCUS       DAOSOX010000107         7899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9159_length_7899_cov_8.348929, whole genome shotgun sequence.
ACCESSION   DAOSOX010000107 DAOSOX010000000
VERSION     DAOSOX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9159_length_7899_cov_8.348929"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLX22_08340"
     CDS             <1..854
                     /locus_tag="PLX22_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19944.1"
                     /translation="IYINIVKNEFIILDNEIKIPRIAVTSLSYDDALTFMPSIVSLIP
                     AFLYNHILLQQRHPANELHSLHFASLLKGELLHFYHILRIDFKFGGDSSSIIQPGSND
                     YYPSYTTNRLYYKSRLIPTLQHSYEPIIPIKLIQSITTESDQFFHTYALFDDVDTTKV
                     THELNTLLHAFMIPENLYPFVVFDYYTACINIVYPDSHELTWAMAVYEPLFLIIASHY
                     IDLNAIIPYHAIKKAFPDFFNIVNDKLIPEDNLLNMIQTYFKRFSFSRDEQCMLKGWW
                     QFVIASQ"
     gene            835..1350
                     /locus_tag="PLX22_08345"
     CDS             835..1350
                     /locus_tag="PLX22_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19945.1"
                     /translation="MLLHRSKLPIIALVLSIACTNQPLSIMKANIGSNVALNGTITNA
                     SLHYTFTPCTWCSTAKDICNYIYFQGDTLCFAVECTTNINDAIVTAYFENPSTKKTYK
                     AERLEKINTTIYGFSLIGSLLEHIYDDTRMKVPCNTWIVLPFVIHITIELQNQKATHS
                     IANTAYISFSK"
     gene            1458..2324
                     /locus_tag="PLX22_08350"
     CDS             1458..2324
                     /locus_tag="PLX22_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT19946.1"
                     /translation="MKKKDLSMFVRFWGVRGSVPTPGQNFIKYGGNTACVELRCGNTI
                     MIFDAGTGIRMLGESLAKEFFDKPIQVHLFISHTHWDHIQGFPFFALAYNKNATINFY
                     GGHSVSTLEKLILGQMDKEYFPVSLFELSSKINFIELSQNAFTINDIVIYYTHLFHPG
                     LSLGFRVEYDDRVFVYASDNEVLNDPELGEYNLKNIGTLIKNADILVADCQYTPNEYK
                     SRIGWGHSSIEIVAQVASRFGVKHLVTYHHDPSHTDDDIAKTIRKVRKLSLPIKITAA
                     QEGTTLYLSKKY"
     gene            2358..2972
                     /locus_tag="PLX22_08355"
     CDS             2358..2972
                     /locus_tag="PLX22_08355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT19947.1"
                     /translation="MHSLQLHNTYLLMRHAQSKANHMHIIVSNSEVGVNDYGLTQEGK
                     NEVMKAIAHCNELKSVEYIYTSDFLRAYETSMIIAGHMNIIHVKFDIRLRERYFGQFE
                     GTDSENYQRIWDMDCCNVHDPVCYDVEPIHTVAKRMISFLLDCETNHINDTILIVSHG
                     DPLQILYTVSIGLPINKFKTASHFNNAELRLLPSRFTIDEELIT"
     assembly_gap    3291..3390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3391..4821
                     /locus_tag="PLX22_08360"
     CDS             <3391..4821
                     /locus_tag="PLX22_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOT19948.1"
                     /translation="YLLGYIGINLYTIGVAFNAMLGWDVMVTAVVIAFVCALYMHAGG
                     QTSVIMTDLAQALMLLTAGILIFILGIDKIGSLKTFWTSLPYEFKLPFSPFNKPPDFN
                     FVGIFWQDAFGSSMAFYFINQGILMRFLSVRSPRDGRKALFLVCLVLMPLAAFVVANA
                     GWIGKSMSIHGLLPSDTNPNHIFVEVVYAITKPGTFGFIMAALIAALMSTIDTLINGV
                     AAIGVNDIVKNIAPDNTDAYYLKWARLISLLAAIIGVALVPLFMQFKSIYVAHGTFIA
                     AVTPPMIVTILFGILWKRYTTQAATYTLIFGSIAMLISIKWPGILTLFSHGVATEGLQ
                     YLRALYGLTICSGISIAMTLLPASKEIKDLPGLVVDTIHEGKVLFKGGEPDDFDEGKI
                     ISSHIVEVNEGVSLSKAMMEHLQAKPGDILYISDERWWLGGLKSIHAKAGPPHDSSDD
                     IILIGTDLIVQGNLDINRKVTAMKII"
     gene            complement(4897..5481)
                     /locus_tag="PLX22_08365"
     CDS             complement(4897..5481)
                     /locus_tag="PLX22_08365"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOT19949.1"
                     /translation="MDAIIAFFSSLFHQYGTLVLIFTARIFDVSIGTVRIILVARGYR
                     SIAPVLGFFEVLIWLFAINSALQHFNGIISYIVYALGFATGNYVGMLLEEKIAIGYQS
                     VRVITSKVVSALPLVLRQEGFGVSQIDGVGMKGPITMLFTVIPKRDVKRVVEIVQMLE
                     PNAFITIEDVKQQLSGFTIHNGFVSKFASLIKKK"
     gene            5968..6264
                     /locus_tag="PLX22_08370"
     CDS             5968..6264
                     /locus_tag="PLX22_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19950.1"
                     /translation="MKPSFITSIVLFIVLYMLIPIHSNEHYQNHTMIDPHFFVKINDD
                     SNAQDEKAQSDATKDPKSLDNPSDDDIFEDDEDENDNDMEDIPDDFYDYQEENL"
     gene            complement(6246..6707)
                     /locus_tag="PLX22_08375"
     CDS             complement(6246..6707)
                     /locus_tag="PLX22_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT19951.1"
                     /translation="MFTKILYPTDFSECANAAIPYIKLMKKTGARELIILHVVDIRYS
                     TIIDTTAFGETFVYPYDISDILKKEAEKQMNNLKKQLSKVYKVTPLIIEGIPFKEIIT
                     IAEQKKVSCIIMGSHGKSNIEEMLLGSVTEKVVRKSTMPCLVVKSHQRFSS"
     gene            complement(6735..7745)
                     /locus_tag="PLX22_08380"
     CDS             complement(6735..7745)
                     /locus_tag="PLX22_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19952.1"
                     /translation="MILRYLSTCTIVLLLITLSCSRQKTVNEGIELYQEGLYQSAFIK
                     IKDGLIQYSMEKTLDEQSFVCTPYAVFSTETLLTMLYPKKHTYEFPREVVSVWYDPAD
                     DVCVVTDGIMVSNFSSDEEIIINNPTTKPIKAVSYCNKDIYLLCGEELYVHSQDDKKI
                     RRLSSIDFPPPETLKYYNGYMHAVNNKLIIIVGIAGMYNAWVLSTEGEILLSRIRIAS
                     WKNILKDDMLFVITGSAGHWDLKSISITTKKDKTIKSFTKLQDVHLLNDGIIINNGDG
                     IVVYNYDNTFYAIPFTYSILGGTNEFLLVDGGYNALISGNKFFMMIEKVVIETGCCII
                     KN"
BASE COUNT         2499 a         1450 c         1261 g         2589 t
ORIGIN      
        1 gcatatatat taatattgtt aaaaatgaat ttataatcct tgataacgaa attaagattc
       61 ccagaattgc ggtaacaagc ctgtcatatg atgacgcatt gacttttatg ccctccatcg
      121 tttcccttat accagcattt ttatataacc atatactgtt gcaacaacgc caccctgcaa
      181 acgaattaca tagcctccat tttgcttctt tgctaaaagg ggagctgtta catttttatc
      241 atattttgcg catagacttt aagtttgggg gcgatagttc atctattatt caacctggca
      301 gcaatgatta ttatccttca tatacaacaa acagacttta ttataaatcc cgactgatac
      361 caacactgca gcattcatat gagccaatta ttcctattaa gcttatacag tctataacaa
      421 ctgaatcaga ccagtttttt catacctacg cactatttga tgatgttgat acaacaaaag
      481 taacccatga attgaatact cttttacatg cattcatgat accagaaaac ctgtatccct
      541 ttgttgtttt tgattactat acagcatgca tcaatattgt ttatcctgat tcacatgaac
      601 ttacatgggc tatggcagta tatgaaccgc tttttcttat aattgcatca cactacattg
      661 atcttaatgc cattataccg taccatgcaa taaagaaagc gttccctgat ttcttcaaca
      721 tagtaaacga taaactgatt cccgaagata acctgctgaa tatgattcaa acttatttca
      781 aacgattttc tttttcacga gatgaacaat gtatgctgaa aggatggtgg caatttgtta
      841 ttgcatcgca gtaaattacc cattatcgca cttgtgttga gcatagcatg cacaaatcaa
      901 ccactttcaa taatgaaggc taatataggg agcaatgttg cattaaacgg aactatcacc
      961 aacgcctctc tgcattatac ctttacacct tgtacgtggt gttcaaccgc taaagacatc
     1021 tgtaattata tctattttca gggcgataca ctatgctttg ccgttgagtg tactactaat
     1081 ataaacgatg cgattgttac tgcatatttt gaaaatcctt ctacaaaaaa aacctataaa
     1141 gcagaacgcc ttgaaaaaat caatacgacc atctatggat tttcacttat cggcagcctg
     1201 ctggaacaca tttatgatga tacacgcatg aaagtaccct gcaacacatg gatagtattg
     1261 ccttttgtta tacacattac tattgaatta caaaatcaaa aggcaacaca ttcaatagca
     1321 aatactgcat atattagttt tagcaaataa aatatatttt tactaagtgt attaattcat
     1381 gtttgaagta ttttctttta tttgtcgatg agttatgtaa tatgatacta tttgcatcaa
     1441 tttaaattta ataaaccatg aaaaaaaaag acctgtcaat gtttgtgcga ttctgggggg
     1501 tacgtggttc agttcctaca ccaggacaaa atttcattaa atatggcggc aatactgcct
     1561 gcgttgagct gagatgtggg aatactatca tgatatttga cgccggtacc ggcatcagaa
     1621 tgctgggaga aagccttgct aaagaatttt ttgataaacc catccaggtt catttattta
     1681 tcagtcatac acactgggat cacatacagg ggtttccttt ttttgcactg gcatataaca
     1741 aaaatgcaac aatcaatttt tatggtggtc atagtgtctc aacacttgaa aagctcattt
     1801 taggacaaat ggacaaagaa tatttccctg tgtcattatt tgagttatca tcaaaaatta
     1861 attttataga gctttcccaa aatgcattta ctattaacga tatcgttata tactataccc
     1921 atcttttcca tccgggatta tcgttaggat tcagggttga gtatgatgac agagtgtttg
     1981 tatatgccag tgataatgaa gttttaaacg atcccgaatt gggtgaatat aatttaaaaa
     2041 atattggcac actgattaaa aatgctgata tacttgtcgc tgattgtcaa tatacaccca
     2101 atgaatataa aagcaggata ggatggggcc attcatctat agaaatagtt gcacaggtag
     2161 catcacggtt tggtgttaaa catctggtaa cctatcatca tgatccatct catactgatg
     2221 atgatattgc taaaactatc cgtaaagtcc gcaaactttc acttcccatc aagattacag
     2281 cagcacaaga aggaacaaca ctgtatcttt caaaaaagta ttgaccatac ttccctgcac
     2341 attatcatta tgctgttatg cacagtttac aattgcacaa tacctatcta ctcatgcgcc
     2401 atgctcaaag caaggcaaac catatgcata tcattgtcag caattctgaa gttggtgtta
     2461 atgattatgg tttaacacag gaagggaaaa atgaagttat gaaagcaata gcgcattgca
     2521 atgaactgaa atcagttgaa tacatctata catcagattt tttacgtgca tatgaaactt
     2581 ccatgattat tgccgggcat atgaatatca tccatgtcaa atttgatata cgtttacgcg
     2641 aacgttattt tggccaattt gagggaacag acagtgaaaa ctatcaacga atatgggata
     2701 tggattgctg caatgtacat gatccagtat gttatgacgt tgaacctata catactgtag
     2761 caaaaaggat gatttctttt ttgttggact gcgaaaccaa tcatataaat gatactattt
     2821 taattgtttc gcatggcgac ccgttacaga tactttatac cgtatctata ggtttaccta
     2881 ttaataaatt taaaacagca tctcatttca ataatgcaga attaagacta ttgccttcac
     2941 gtttcaccat tgatgaggag ttgatcacat gatgtcacca tggttaccat tcatttacca
     3001 atatactatt tgctcaattt tttttattgc tatgattgta ctatccatta agaaaaaagc
     3061 catttctatt caccacccaa ctgacagaaa aattttatta gaaattatta gcggttatct
     3121 tatatttatt tttgctcatg ctttgatgat actaattgca ggagcttagt tatgattaaa
     3181 ggaacaacca tcgattttgc aataattgta atatacttta ttgttatcct tggctttggt
     3241 tcattttttg gtaaatatgt caaaagcaca aaggatttct tttttggcgg nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacttattag gctatattgg aataaattta
     3421 tatacaattg gtgttgcctt taatgctatg ctaggctggg atgtgatggt aactgccgta
     3481 gttattgctt ttgtatgtgc tctgtatatg catgcgggcg ggcaaacttc agtaataatg
     3541 accgatttag ctcaagcctt aatgctattg acagcaggaa ttttaatatt tattcttggt
     3601 atagataaaa ttggcagttt gaagacattc tggacatcac ttccttatga atttaaactg
     3661 cccttctcac cttttaacaa acctcctgac ttcaactttg ttggcatttt ctggcaggac
     3721 gcatttggca gttcaatggc attttatttt ataaatcagg gaattttaat gcggttttta
     3781 agtgtacgct cacctcgtga cggcaggaaa gccttgtttt tggtttgcct tgtattaatg
     3841 ccattagcag cgtttgttgt cgcaaatgca ggatggatag gcaaatcaat gtcaatacat
     3901 ggacttctac cttctgatac aaatcctaat catatctttg ttgaagtagt atatgcaatt
     3961 acaaaacccg gaacatttgg ttttattatg gcagctttaa ttgctgcgct tatgtctacc
     4021 attgacacat taatcaacgg tgttgctgca attggtgtta atgacattgt aaaaaatatt
     4081 gcacccgata acactgatgc ttactatctc aaatgggcac gtttaatatc gttattagct
     4141 gcaattattg gtgttgctct tgttccctta tttatgcaat tcaaatcaat ctatgttgct
     4201 catggaacgt ttattgctgc tgttactcca cccatgatag ttaccatact atttggtatt
     4261 ttatggaaaa gatacaccac acaagctgcc acatatacgt taatttttgg aagcatagca
     4321 atgctcatat ccattaaatg gcctggaata ttaacgttat tttcacacgg tgttgctacc
     4381 gagggactac aatacttgcg agctttatac ggacttacca tatgttcagg aatcagcatt
     4441 gccatgacat tattacctgc ctcaaaggaa ataaaagacc tgcccggtct tgtggtagat
     4501 actatccatg aaggtaaggt tttatttaaa ggcggcgaac ctgacgattt tgatgaggga
     4561 aaaataatct ccagccatat tgttgaagta aatgaaggtg tgtcactaag caaagcaatg
     4621 atggaacacc tgcaggcaaa acctggcgat atactgtaca tttcagatga acggtggtgg
     4681 ctgggtggac ttaaatccat tcatgcgaaa gccggtccac ctcatgatag cagtgatgat
     4741 atcattctta taggcactga tcttatagtg cagggaaatc tcgatataaa tcgcaaggtt
     4801 acagcaatga aaataatttg atagtaacta tcgccctcta caaaacatag aatatataat
     4861 ttttgttttt aaatgtatag atcaaaaaaa tataaattat tttttcttta ttaatgaagc
     4921 aaactttgat acaaatccat tatgaatagt aaaaccactc aattgctgtt taacatcttc
     4981 tatggtgatg aatgcattgg gttctaacat ttgaactatt tcaaccactc ttttcacatc
     5041 tctttttggt atgacggtaa acaacatcgt tataggccct ttcatgccaa caccatcaat
     5101 ttgacttact ccaaaaccct cctgcctcaa taccagagga agtgcagaaa cgaccttgct
     5161 ggtaataact cggactgatt ggtacccaat ggctattttt tcctctaata acatcccaac
     5221 ataatttcct gtggcaaaac ctaatgcata aactatataa cttataatcc cattaaaatg
     5281 ttgaagtgca gaattaatcg caaaaagcca gataagaacc tcaaaaaatc ccagcactgg
     5341 tgctatagac cggtatcctc gagcaaccaa aattattcgc actgttccaa ttgacacatc
     5401 aaatattctg gcagtaaata ttaatactaa ggtaccatac tgatgaaaca aagaactgaa
     5461 aaaggcaatg attgcatcca tagcgaaata ttattatctc ctaccatgtc tgatatatct
     5521 cttgaaaaat atatatatgt atcaatgtca atatatattt tacatacatg ttacaggcat
     5581 gttgagtata atatgatgaa ctatacaaca aaaaaattat tgcaattacc actacattat
     5641 ggcataaatg tatcaacatt aatcattcaa acaccgtcag gagtaacagt ataccataag
     5701 aatctaaagc caaaaagcca gataccggat caagtccggg atgatattac ggtggaatgt
     5761 catccagaat tcatttcagg atctaacgtt atatatctgg atcccgtgtc aagcccggaa
     5821 taacagaggt gttattgcca tttttgtaat agttcatgat ggccggttta taactaccag
     5881 caggaacgaa aatatttacc gtttgtcatc gtgaatcctg attaagaata acatgggcat
     5941 ataatcttat tttcagaggc atgttttatg aaaccttcat ttattacatc cattgtgttg
     6001 tttatagtat tatacatgct tatcccaatc cacagcaatg aacactatca aaaccataca
     6061 atgattgatc cacatttttt tgtaaagata aacgatgact caaatgccca ggatgaaaaa
     6121 gcacaatcag atgctactaa agacccaaaa tctttagata atccttctga tgatgacatt
     6181 tttgaggatg acgaagatga aaatgataat gatatggaag atatccctga tgatttctat
     6241 gattatcagg aagagaacct ttgatgactt ttaacaacta gacagggcat cgtcgacttt
     6301 cttaccactt tttcagtgac agatcccaat agcatctctt caatattgct tttgccatgc
     6361 gaccccatta ttatgcatga aaccttcttt tgttcagcaa tagtaataat ctctttaaac
     6421 ggaatacctt caataatgag aggagtaact ttgtatacct ttgataattg tttctttaaa
     6481 ttattcatct gtttttcagc ttcttttttc agaatatcag aaatatcata gggataaaca
     6541 aatgtttcac caaatgctgt ggtatcaatt attgtgctat aacggatgtc aactacatga
     6601 agaataataa gctcccttgc ccctgttttt ttcattaact ttatgtaagg aatggctgca
     6661 ttagcacatt ctgaaaaatc tgttggatat aagatttttg taaacatttt gttgtccttc
     6721 taagattaat gtatctaatt ctttatgatg caacaaccag tttcaataac aactttttct
     6781 atcatcataa aaaatttatt accacttatc aacgcgttat agcccccatc taccaaaaga
     6841 aattcatttg ttccacctaa tatgctataa gtaaatggta ttgcataaaa tgtattatca
     6901 taattataaa ctactattcc atcgccgttg tttatgataa taccatcatt aagcaaatga
     6961 acatcctgca gttttgtaaa gcttttaatt gtcttgtcct ttttcgttgt gatactgata
     7021 ctttttaaat cccaatgtcc agcactaccg gtaataacaa acagcatatc atcttttagt
     7081 atattcttcc atgaggcaat ccgtatacgg gatagcaaaa tctccccttc agttgataac
     7141 acccatgcat tatacattcc ggctattccc acaattatga tgagcttatt attgacagca
     7201 tgcatatatc cattgtagta tttcaatgtt tccggtggtg ggaaatctat agatgacaat
     7261 cttcttattt ttttatcatc ctggctatgt acatataact cttccccgca gagcaaataa
     7321 atatccttat tgcaatacga aactgcttta atgggtttgg ttgttgggtt attgataatg
     7381 atttcctcat ctgaggaaaa attagatacc ataatgccat cagttacaac acaaacatca
     7441 tcggcaggat cataccatac actaactact tcacgtggga attcatatgt atgttttttt
     7501 gggtacagca tggtgagtaa tgtctctgta gaaaaaactg catatggagt acaaacaaaa
     7561 ctttgctcat ccaacgtttt ttccatagaa tactgtatta gcccgtcttt aatttttata
     7621 aaggcacttt gatataaccc ctcctgatac agttcaattc cttcatttac cgttttttgc
     7681 cttgaacatg atagagtaat aagtaaaaga acaatagtgc acgttgaaag gtaacgaaga
     7741 atcatgttta acctttttta cctttaattt tttccattgc cttttgtaaa aatcgcccat
     7801 ccatgatttc tatcatttta tttgctgcct gagatttagc attaatacta aattttgatg
     7861 ttgttatata aataccactt ttacacttct cttcaagca
//
LOCUS       DAOSOX010000108         7812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9291_length_7812_cov_7.406472, whole genome shotgun sequence.
ACCESSION   DAOSOX010000108 DAOSOX010000000
VERSION     DAOSOX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_7812_cov_7.406472"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..2680
                     /locus_tag="PLX22_08385"
     CDS             176..2680
                     /locus_tag="PLX22_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOT19953.1"
                     /translation="MPIDTALTSVLIHSGMMQFLKDGQPAVNLRNRLQKPCPLVLDGA
                     MGTMLFDALPDYNGSLELLNMERPDVIISIHKQYIDAGADIIETNTFGGNALKLQQAN
                     LDSRCEEINKAAAVIAKQAASNRAFVAGSVGPTGTLVEPMGTTPDEAVYNAFKKQIKG
                     LVDGGVDCIAIETMTDLQEARLALLAAKAVTNIPIICSMTFEDNGKTVTGTDIVTAFA
                     TLSQMGADVVGANCSMGPDGLVQLFTGAIAQLKQLGVPLSVWANAGLPTLVNGKATYS
                     LSPQQFAMLSTQFATMGFSIIGGCCGTTPAHINALANAVKEIRIDIPRSKTFHYITSR
                     YGYIDVQQHQGLIIIGERLNPTARKQFAQELKENKFSFLREESKKQQDEGAHILDINV
                     GVPGIDESIMMTKAVTILSKTVTIPLMIDSDNHAVIQAALWHYPGIPILNSISAKEES
                     FNSMLPLLQQSGAFVIAMCLDESGIHKEAKKRIAIGQRLITLLQDNGIELSRIFIDPL
                     ILAESAESGAAMETLKVIEYFSKHGIKTSIGLSNISFGLPQRKYINNAFLKLAIQKGL
                     TAAIVNPSAIDSNTEKPFTAEETMALDFLEGRDKNAVHYIAHFGTSQQQSRIQSPVKE
                     KSSDILNRIFSLVVEGNIDDIEHAIHQALTMYEPEIIMSNALIAALEKVGQLYSSGEY
                     FLPQMIASADTMKKGFTVLKPLMQQHKESLLGKVLICTVRGDIHDIGKNIVAMMLENH
                     GFDIIDLGKDVPNEAVIASIIEHKPDIVCLSSLLTTTMPSMEQIATMIKNQQLPCKVM
                     VGGAVVTKEYAESIGAYYGKDAVDAVSIAKTIVNVK"
     gene            2870..3385
                     /locus_tag="PLX22_08390"
     CDS             2870..3385
                     /locus_tag="PLX22_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOT19954.1"
                     /translation="MKGTRSFLLFFLPFIIFTYIAFIVFQQACDYAMNVQAKKLLPFN
                     HQTHTTQYGADCELCHSYDANGRFNGIPTVAVCKQCHDPNKVNNAVFFAGFNDTDKPW
                     ETFAQQPDLVYFSHIAVTKNEQKVQCISCHGDKGASKTTAKIKGKMAMGVCMDCHTVT
                     NVSNSCTVCHD"
     gene            3434..5404
                     /locus_tag="PLX22_08395"
     CDS             3434..5404
                     /locus_tag="PLX22_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT19955.1"
                     /translation="MKLERKDFLTMIGGAVVGTGVGTLISPGPWMGLQWLVEWTQDQY
                     RPPKGEEKYLASICDRCPDKCTIQVRKIGNRAVKIETSNQGCAVGQLLLQVLYHPQRV
                     TTPLKRTGKKGKIDFAPVSWDEAIADISKKINDAINNNKQEQIIAINPAYPDATSMLT
                     ERIALFSGSPYVFQESGYWALSNACSEIAFKTDGAIDYDFENADCVVSFGARLFEGWG
                     NPGRMHNVLSLWKKKGIKFIQIDSIATRTASLADQWVAIKPGSEPLFAMGVAHYLMKY
                     GKRSGATNFGRFTELLINKYTPEKVSELTGVSLDTIESVAKALISAQRPVAVAGRGGK
                     VNSSSVAEFAIIQTINSMIGSMGSSVKLVSFTTPAKPVIPKKAKGLDEYIASDSVCDI
                     ALINNANPVYSSVYGQRLSDKLKKASTVVLCTSLLNDTALYADYVFPTLTSLELPVAG
                     NAVVTPTSEAKHAGDSLLAIAKNIAKISSQFSWKNYLETVQTVKYQTKNIANVDIPVD
                     LIITKLEETEKILSASGSYSIAMIPFEIPTVSDGSGLSLPYVLKGLPGTILKGDTSWV
                     LMNPATASKEGISEGSKIDIESSRGELDTVRVHLTKRIAYDTVAIPIGFGHEEFTDYA
                     SEKGVNPKIIMSDAIDPASGYADWWLTRVKLS"
     gene            5418..6248
                     /locus_tag="PLX22_08400"
     CDS             5418..6248
                     /locus_tag="PLX22_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT19956.1"
                     /translation="MAKLTRRWGMVIDLDKCTGCGACNIACSQENNLPIFKDDSDIPK
                     RVTFLDLVEVTNDKDPKAQYPDVKVAYLPKMCQQCSGNDPNHPNPPCVSVCPVVATDV
                     GEDGVVSQIWSRCIGCRYCQVACPYEARVFHWWKPRYEGSFKKSLNPDVSIASRGTMV
                     KCTFCSHIWKRERDRAIANGVTDINAVTYTPACASACPTNAIIFGDLNDPSSPVYEAA
                     VQKNPRAVRLVHSIDELRTKDPSEYEKRLKIKDYPNPKVYYLTSQQWLRDKISGFKNS
                     "
     gene            6272..7501
                     /locus_tag="PLX22_08405"
     CDS             6272..7501
                     /locus_tag="PLX22_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19957.1"
                     /translation="MSKVINFLKDEWNSYSRNIQISLYVLFALVALFVIFGIQILFWG
                     LGYTDMNNRFAMGLWIIGDLGFVALGGGAFFTGFLLYIFRVDKLQPLINSTVLIGFMC
                     YLFTFILLVFDIGQPLRAWFGYAYPNWGPHAMPQSMLTEVVWCLTFYFIVLCIELFPI
                     ILSNKYLDRYPAVHYFAHYLHKLMWIFAAIGTFLSFFHQGSLGGGMWGVLYAKPYWFR
                     HHYFYLAIVAAIAGGPCLMMLVPKIASWVAKKELVPRDAYMTLAKVAGVTFIAYFLFR
                     IYDIYSLWTHFAPLFDRSFIEMMGGYYGWWVVTLELLASLTAIVILNVKKFRENEQYF
                     IAGLISGVFAIVMLKVDVLLHGFSIPNFSWEGFFTYNPTIQEWSIFFGSIACMILIYM
                     WFTKYIPLFPEKHTEAH"
     gene            complement(7595..>7812)
                     /locus_tag="PLX22_08410"
     CDS             complement(7595..>7812)
                     /locus_tag="PLX22_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOT19958.1"
                     /translation="VKPGAVVIDVGINRIDDPRNPGKTKLVGDVKFDEVSEIASAITP
                     VPGGVGPMTIVMLMKNTVKAFRLQNKI"
BASE COUNT         2406 a         1452 c         1622 g         2332 t
ORIGIN      
        1 agggatgtta ttcagagcaa attggcatca tgagattgca taaacaataa ataatcataa
       61 atactattga caccggaacg tattattgtg tcgtaatcat aaaatgtgaa tttttttatt
      121 gtatattttt ccattctatt ttttcttgtg cgatagttcc tgataacagc tttttatgcc
      181 catagacacg gctttaacct ctgtattgat acacagtggt atgatgcaat ttttaaagga
      241 tggtcaacct gcggtgaatc taagaaaccg actgcaaaaa ccctgccccc ttgtgctgga
      301 cggagcaatg gggaccatgt tatttgatgc tttgcctgat tacaacggca gccttgaatt
      361 gctcaatatg gagcgtcctg atgttattat ttcaatccac aagcaataca ttgatgctgg
      421 agctgacatt attgaaacca atacatttgg cggtaatgct ttgaagctgc aacaggcaaa
      481 tctggatagc cgttgtgaag agataaataa ggctgctgcc gtcattgcaa aacaggccgc
      541 aagcaacagg gcgtttgttg ccggctcggt tgggcctacc ggcaccctgg tggaaccaat
      601 gggcaccacc cctgatgaag ccgtctataa tgcatttaag aagcagatca aagggttggt
      661 agatggcgga gtggattgta ttgccattga aaccatgacc gacttgcagg aagcacggct
      721 ggctctgctt gctgcaaaag ctgtcacaaa tattcccatt atatgcagca tgacatttga
      781 agataacggc aaaacagtta cgggaacgga tatcgttaca gcgtttgcca ctctttcaca
      841 gatgggtgcc gatgtggtgg gtgccaattg cagtatggga cctgatggtc tggtgcaact
      901 ttttacagga gctatcgccc agcttaaaca acttggcgta ccgctgtcag tgtgggcaaa
      961 tgcaggtctg cccacgcttg tcaatggaaa ggctacctat tcactatcgc cacaacaatt
     1021 tgcaatgcta tcaactcaat ttgccactat gggattttcc attattgggg gctgctgcgg
     1081 aaccactcct gctcatatca acgctttagc aaatgctgtg aaagagatta gaatagatat
     1141 tccacggtca aaaacatttc attacattac atcgcgctac ggatatattg atgtgcagca
     1201 acatcaggga ttaattatca ttggcgaaag gttaaatcct acagcgcgca aacagtttgc
     1261 acaggaatta aaagaaaaca aatttagttt tttgcgtgag gaatcaaaaa agcaacagga
     1321 tgaaggagcc catattctgg acatcaatgt aggtgtaccc ggcatcgacg aaagcatcat
     1381 gatgacgaag gctgttacta tcctatctaa aacagttacc ataccattga tgatcgattc
     1441 ggacaatcat gctgtcattc aagctgcgct atggcactat ccgggtatcc caattttaaa
     1501 ttctatcagc gcaaaagagg aaagttttaa cagtatgctt cctttattgc aacaatctgg
     1561 cgcttttgtc attgcaatgt gtcttgatga aagcggcatt cacaaagaag caaaaaaacg
     1621 aattgccatc gggcaaaggc ttattaccct gttgcaggat aatggcatag aactatcacg
     1681 catatttatt gatccgctta tacttgccga aagcgctgaa agcggcgctg caatggaaac
     1741 attgaaagta atagaatatt tttcaaaaca tggtattaaa acaagcatag gacttagcaa
     1801 tatttcgttt ggacttccac agcgaaagta cataaacaat gcatttttaa agttagcaat
     1861 tcaaaaaggg cttacagcag ccattgtcaa tccttctgct atcgacagca atactgaaaa
     1921 accttttact gctgaagaaa caatggcact tgattttctt gaaggcagag acaaaaatgc
     1981 cgtgcactat attgcccatt ttggaacatc acagcagcaa tccagaatac aatccccagt
     2041 aaaagaaaaa tctagtgata tactgaatag aatcttttca ctggttgttg agggcaacat
     2101 tgatgatatt gaacatgcaa ttcatcaggc attaaccatg tatgagccgg aaatcataat
     2161 gagcaatgcc ctcattgctg ctctggaaaa agtggggcaa ttgtacagta gcggtgaata
     2221 ttttttaccg caaatgatag cctctgctga tacaatgaaa aagggtttta ctgtactgaa
     2281 acctctgatg cagcaacata aagaatccct gctgggaaag gtgctcatat gcactgtcag
     2341 gggcgatatt cacgacattg gcaaaaatat tgtagcgatg atgctggaaa atcatggatt
     2401 tgatatcatc gatctgggaa aagatgttcc caatgaagct gtcattgcct ccattattga
     2461 acataaaccg gatattgttt gtttaagctc actgctgacc accaccatgc ccagcatgga
     2521 acagattgca accatgatca agaaccagca attaccctgc aaggttatgg ttggtggggc
     2581 tgtagtaaca aaagaatatg ctgaaagcat tggcgcctat tatggcaaag atgccgttga
     2641 tgctgtaagc atagcaaaaa ccattgttaa cgttaaataa gcagtaattt aaaataatgg
     2701 atttacactg ctttcccttc catttttttt tataattttg ttgacattaa gattgtatat
     2761 acaagaattc gcatatgtac ttattctgtg gattaaatac aatccacttt ttttaaatat
     2821 ttttattttt aaagtatcta taccactata aagattaagg agcacgtctg tgaaagggac
     2881 acgttctttt ctgctttttt tcttaccttt tattattttt acatacatcg catttattgt
     2941 atttcagcag gcctgtgatt atgcaatgaa tgtacaggca aaaaagctcc ttccattcaa
     3001 tcatcaaact cacacaactc agtacggtgc tgactgtgaa ctatgccaca gctatgatgc
     3061 gaatggaaga tttaatggta tccctacggt agcagtatgt aagcaatgtc atgatcctaa
     3121 caaagttaat aatgcagtat tctttgcggg atttaatgac actgacaagc catgggaaac
     3181 atttgcgcag cagcccgatt tagtatattt cagtcatata gctgtgacaa aaaatgagca
     3241 aaaggttcag tgtatttcgt gtcatggcga caaaggagct tctaaaacaa ctgctaagat
     3301 caagggtaaa atggcaatgg gtgtatgtat ggactgtcac acagttacaa atgtaagcaa
     3361 ctcgtgtaca gtatgtcatg attaatcatt cttattttat gaattgaaaa acaagtaagg
     3421 agctattaca tccatgaaac ttgaacgaaa agacttctta acaatgattg ggggggcggt
     3481 tgttggtacc ggtgtaggta ccctgatatc acctggccca tggatggggt tacagtggtt
     3541 agttgaatgg acacaggatc agtatcgccc accaaagggt gaagaaaaat acttagcaag
     3601 tatatgtgac aggtgccctg ataaatgtac cattcaggta cgcaaaattg gcaacagagc
     3661 agtcaaaatt gaaacttcaa accagggttg tgctgtaggg cagcttttgt tacaggttct
     3721 ttatcatccc cagcgagtta ccactccatt gaagcgtacc ggtaaaaaag gtaaaattga
     3781 ttttgcaccg gtatcatggg atgaagcaat tgctgatatt tccaaaaaga tcaatgatgc
     3841 tatcaataat aataaacagg aacagattat agccattaat cctgcatatc ctgatgcaac
     3901 ctcaatgttg accgagagga tagcgttatt ttccggtagc ccatacgtat ttcaggaatc
     3961 aggttactgg gcactcagca atgcatgttc agagatagcc tttaaaactg atggcgctat
     4021 tgattatgat tttgaaaatg ctgattgcgt tgttagtttt ggtgctcgtc tttttgaagg
     4081 atggggtaac ccaggaagaa tgcataatgt attgtcatta tggaagaaaa aaggtattaa
     4141 atttatacaa atagactcaa tcgcaactag aactgcttca ttggctgacc agtgggtggc
     4201 aataaaacca ggcagcgaac cgctatttgc tatgggtgtt gctcattacc tgatgaaata
     4261 tggtaaacga tcaggtgcga ccaattttgg aagattcact gaattgttaa tcaacaagta
     4321 tacacctgag aaggtttcag agctaaccgg agtttcatta gatacaattg aatcagtagc
     4381 aaaggctctt atcagtgctc aaagaccagt tgctgtagct ggtagaggag gaaaagttaa
     4441 ttcctcttcc gttgccgagt ttgcaataat acagaccatc aacagcatga ttggaagcat
     4501 gggaagcagt gttaaactgg taagttttac aactccagct aaacctgtta taccaaaaaa
     4561 agcaaaagga ttggatgaat atatagcaag cgatagtgtc tgtgatatag cactaatcaa
     4621 taatgccaat cctgtgtact caagtgtata tggtcaacga ctatctgata agcttaaaaa
     4681 agcttcaacg gttgttcttt gtacatctct gctcaatgat acagctctct atgcagatta
     4741 tgtttttcca acattaacat cgttggagtt gcctgttgca ggcaatgcag ttgtgacacc
     4801 tacatcagaa gctaaacatg ctggggattc actgcttgca atagcaaaaa atattgcaaa
     4861 aatatcatcg caattctcat ggaagaatta cttagaaacg gttcaaaccg ttaaatatca
     4921 aacaaaaaat attgctaatg tagatatacc ggtagatttg attattacaa aattggaaga
     4981 aactgaaaaa atactcagtg catcgggttc atactcaata gcaatgatac cttttgaaat
     5041 accaactgtt agcgatggta gtggattatc actgccgtat gtattaaaag gtttacctgg
     5101 tactattctg aaaggtgata caagctgggt gttgatgaat ccagcaactg cttctaaaga
     5161 aggaatttcc gaaggttcca aaatcgatat agaatcttcc cgtggtgagc ttgacacagt
     5221 acgagtccac cttacaaaac gaattgctta cgatactgtt gcaattccaa tcggttttgg
     5281 tcatgaggaa tttactgatt atgccagtga aaaaggtgtt aaccctaaaa ttatcatgtc
     5341 agatgctatc gacccggcca gtggttatgc cgattggtgg ttaacacgag taaaattgag
     5401 ttaagaggtg aataattatg gccaaattga caagaagatg gggaatggta attgatttag
     5461 ataaatgtac tggatgtggg gcatgtaata ttgcctgttc ccaggaaaat aatctaccca
     5521 tttttaaaga tgattctgat atacctaaac gggttacctt ccttgattta gtagaagtaa
     5581 ccaacgataa agatcctaaa gcacaatatc ctgatgttaa ggttgcatat ttacccaaga
     5641 tgtgtcagca gtgttcaggt aatgacccca atcatccgaa cccaccatgt gttagcgttt
     5701 gtccggtcgt ggcaactgat gtaggcgagg atggtgttgt tagccagata tggtcacgtt
     5761 gcataggatg cagatactgt caggttgcat gtccgtatga agcccgcgtc tttcactggt
     5821 ggaaaccgcg ttatgaaggt agcttcaaaa agtccttaaa tcctgatgtt tctattgcat
     5881 cacgaggaac catggtgaaa tgtactttct gttcacatat atggaaacgt gagcgtgata
     5941 gagctattgc taacggtgtt actgacatca acgctgttac ctatacacca gcatgtgcat
     6001 ctgcatgtcc aacaaatgca ataatctttg gcgaccttaa tgatccctca tcaccagtat
     6061 atgaagctgc tgtacagaag aatcccagag cagttcgtct tgttcattct atagatgaat
     6121 tacgaacaaa agatccttct gaatatgaaa aacgattgaa aattaaagat tatccaaatc
     6181 caaaagtata ttatctcaca agccagcaat ggctgcgtga taaaatatct ggatttaaaa
     6241 actcctgaaa aaggcaaaag gagagttaac cgtgagcaaa gtaatcaatt tcttaaaaga
     6301 tgaatggaac agctattcca ggaatataca gatatcatta tatgtgctgt ttgccctggt
     6361 agcgttattt gtcatttttg gtatccagat attattctgg ggattaggtt ataccgatat
     6421 gaataatcga tttgctatgg gattgtggat tatcggtgat ttaggttttg tagcactggg
     6481 tggcggagct ttttttacgg gcttcctgct atacatattt agagttgata aattacagcc
     6541 tttaattaac tcaactgtgc tgataggttt tatgtgttat ctttttactt tcatattatt
     6601 ggtatttgat atcggtcagc cactaagagc atggtttggt tatgcctatc ccaattgggg
     6661 tcctcatgcc atgcctcaat caatgttaac cgaggtagta tggtgtttaa cgttttattt
     6721 tattgttctt tgtattgagc ttttcccaat tatactgagc aataaatacc ttgatagata
     6781 ccccgcagta cactattttg ctcattattt acataaactt atgtggattt ttgccgctat
     6841 tggaacattt ctttctttct tccatcaggg atccttaggt ggcgggatgt ggggagtgtt
     6901 atatgcaaaa ccatactggt tcagacatca ctatttttat ctggcgatag ttgctgcgat
     6961 agcgggtgga ccctgcctca tgatgttagt tcctaaaata gcaagctggg ttgcaaaaaa
     7021 agaattagta cccagagatg cctatatgac attagcaaag gttgcaggag ttacattcat
     7081 tgcgtacttt ttgttcagaa tatatgatat atactcatta tggacccatt ttgcaccact
     7141 ttttgacaga agctttatcg agatgatggg aggctattat ggttggtggg ttgttactct
     7201 agaactttta gcttcgctaa ctgcgatagt tattcttaat gttaaaaaat ttcgtgaaaa
     7261 tgagcaatat tttattgcag gtttgataag tggagttttt gcaatagtga tgttaaaagt
     7321 tgatgtatta ctccatggat tttccatacc aaacttttca tgggaaggtt tctttactta
     7381 taatccaacc attcaggaat ggtcaatatt ctttggatca attgcatgca tgatactcat
     7441 ctatatgtgg tttacaaagt atataccgct attccctgaa aaacacacag aagctcatta
     7501 aaacagtatt cgagtaaata ttaatacaat aaaaaagggt gttccattac accctttttt
     7561 attgttgctg aaatgcaatg ttgccacatc attgttatat cttattttgc aatcgaaaag
     7621 ctttcactgt atttttcatc aacataacta tcgtcattgg ccccacacct cccgggacag
     7681 gggtaattgc agaagctatc tcacttacct catcaaattt aacatcgcct accagttttg
     7741 ttttacctgg attacgggga tcatctattc tgtttatccc cacatctatg acaacagcac
     7801 ctggtttaac ca
//
LOCUS       DAOSOX010000109         7726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9433_length_7726_cov_7.852301, whole genome shotgun sequence.
ACCESSION   DAOSOX010000109 DAOSOX010000000
VERSION     DAOSOX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9433_length_7726_cov_7.852301"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLX22_08415"
     CDS             <1..491
                     /locus_tag="PLX22_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT19959.1"
                     /translation="TADTVYCSIAVTVYVLIALSDIVDGYLARYLHQVSRIGQFLDPI
                     ADKLTIFILSLILYIFKGFPLWLVIVIVVRDIGHIAAGAILFCTHDIQVRPSNAGKCM
                     IFILGMTGFIYIINLSYSVMGISVKHIFVALTTALIVLSTVVHWNIYSKIYFTSKSVS
                     SK"
     gene            545..1681
                     /locus_tag="PLX22_08420"
     CDS             545..1681
                     /locus_tag="PLX22_08420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT19960.1"
                     /translation="MLLTVCLLPLFPQDSIVIPVKIINSNTYVSEYDIRTILPLQSTF
                     DIVLQRGRIVNKERYALYHVGLAIALVNDSLVRSDYPVMRRHGEVMIPLEIAFTMLTA
                     LLPEYEVDIKDNAITLKKIPEYVKEKEKKTPVTKVSKDAIGFIVVDAGHGGKDPGAIG
                     KGGIKEKHITLQIAKEVKLVLQQRLPGIEVILTRTTDRFIELGERTEIANTMLKKGVN
                     GLFISIHCNAAVVSKISGFETYYLSQNPSNEEARTTSVLENNVIIFENPEKRKRYGDV
                     EMIEALMITTQIQKESAMLAASVQRYLDKTIHEFKSKGVKKADFFVLRGCLMPSVLVE
                     VGYITNPKEKKYLIDKNYQKRLAQGITAGIVAFINEYNRNIANY"
     gene            1690..2349
                     /locus_tag="PLX22_08425"
     CDS             1690..2349
                     /locus_tag="PLX22_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19961.1"
                     /translation="MRLGVKNFITRHKLDTLPGILKMKLLTYYQAIAAGSNKLMDLCL
                     YWICNPPLDTKKRYRALLLAISVVCIVDYCAISVLDDKNLFDIYPSLPALDTRKTIDI
                     FVADIDAKSILKENRRVLLPQDDEGKVNRLLKLVAEGSEFENTRAIAPVDIILRHIWF
                     YDTTCIIDCELSTIEGHVPSIADSFQAFTKALTQTICHNVKGVTKVEVLEHGIYGKNL
                     W"
     gene            complement(2375..3205)
                     /gene="dapF"
                     /locus_tag="PLX22_08430"
     CDS             complement(2375..3205)
                     /gene="dapF"
                     /locus_tag="PLX22_08430"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT19962.1"
                     /translation="MPFTKLHGIGNDYIYINAIKHSVKNPKKLAIAMSNRNFGVGSDG
                     LILILPSDKADFTMRMFNADGSEAEMCGNGIRGFAKYVYDHNLTKKTTIAIETLAGIK
                     QVRCTLKNGKVHTVTVDMGEPILLRDKIPMIGNPGTVIEEDLHVDGVKFSITAVSMGN
                     PHVVIYVEDVKNFPVEKYGPMIENHELFPKRTNVEFVQIVTGKEVIQRTWERGSGETL
                     ACGTGASAVTVAGVLTEKTDRRLKVHLKGGNLAIEWNNKDNHVYLAGPAEEVFEGVWP
                     "
     gene            3398..>3699
                     /locus_tag="PLX22_08435"
     CDS             3398..>3699
                     /locus_tag="PLX22_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19963.1"
                     /translation="MQRYNLNNRKFSKKGYAGTGGFFTRIVHFFEDVLYALELRFGNI
                     RPIIIGLIAGAIIAFCAILVIDFYKVRALAHYTPYQTTKIYDKNNVLVAEFFKEKR"
     assembly_gap    3700..3799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3800..6075
                     /locus_tag="PLX22_08440"
     CDS             <3800..6075
                     /locus_tag="PLX22_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT19964.1"
                     /translation="IYDKNNVLVAEFFKEKREVVPLKKIPPHLIQAFIAMEDNEFYDH
                     FGINPKGIVRAFFVNIVSGRVKQGGSTITQQLSKILLTSGERSIYRKVKEAFISIMIE
                     MFYTKDEIMEMYLNQIFLGHGAYGVESASKFYFGKHVWEINIAESSLLATLPSAPNIL
                     SPIRNPRLSMERHRIALAKMVDMGFITVAQAEKAYLDFWPDYLAYINDLPPTMTTWSS
                     RVNKAPWFTEHIRRILVNKFGEKAVYEDGLAVYTTVDVDKQQCAQSLLKDAVEKQTTV
                     SRGLAFDNEEYITNRYADEVNLLTIMFGLPAIKSAGTAQKRTFNRYMKDGLLHEMQMI
                     NLITGLDTIDDVLQQYFLGSYDQKEFMNVEGCLVSINPGNGYIEAMVGGSDFTSTNQL
                     NRVMQSMRQAGSAIKPLLYIAAIDSKKFTAATTIMDSPILYLDMEGGDWIPENYEGEY
                     YGLVTLRRALSLSINVVSVRIADTLGIDTVLNNYAKLLKISRNEMKNRVPRNFSIALG
                     SFEVSPLELARAYAIIANGGKNVIPYSIRYVQDKTGKIIYNQEEEIMAELAEQEKDGS
                     IQIVSPDAARIVVSLMQSVISSGTGRGAAIGRPVAGKTGTTSNYRDAWFVGFTPQLVT
                     GIWMGYDGRGLSLGPGQSGGAVVAPVWGRYMSCALQKEPVLDFAPYGNIAVTKVCAES
                     GLLPSYYCKKVIDELFIPGTEPQEECAKCAGGISLPTTKIPHINVSEKQKEQIMKKMK
                     RKDTEDILDSIENTLKQ"
     gene            6093..6656
                     /locus_tag="PLX22_08445"
     CDS             6093..6656
                     /locus_tag="PLX22_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOT19965.1"
                     /translation="MDLKNFYIEAQDTALFVIDVQDSLFNSMEYEMRRKVAKNVAILI
                     ELAKQYNLPIVVTEQYRKGLGSTIKEVMDKLEGVVPFDKLTFDCMKDDSIREQVAGLN
                     KRNLIVCGIETHICVLTTSLSLMKAGYNVVIASDAVCSRRTHEWDMAIQTLRQAGAVV
                     YPTETIAFMMLEKAGTEIFKKLSPLFK"
     gene            complement(6670..7413)
                     /locus_tag="PLX22_08450"
     CDS             complement(6670..7413)
                     /locus_tag="PLX22_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT19966.1"
                     /translation="MFTLVSTKSVASDHYLLTLQSEVLASQPGQFINIKVTNNTDPLI
                     RRPFSIFDHSGTNLQIVVRVVGKATRQIAALHPGAIDCIGPSGKGFAIVTGKKALLIG
                     GGVGNAPLFYCAKKLREHNSTVTYIYGATSQNTIYMLDDYKNISNELIVVTDDGSAGV
                     EGNPVDIARAIIKTNSFDMIYTCGPEIMMQRITQELSPIPIQASLERYFGCGIGLCSG
                     CAVATKAGYKRACVDGPVFDGYDVLWDKP"
BASE COUNT         2328 a         1381 c         1631 g         2286 t
ORIGIN      
        1 ctactgctga cacagtatac tgctcaattg cagttacagt gtatgtattg atagctctat
       61 ctgacattgt agatggatat cttgcacgct atttgcatca ggtttcccgc attggacaat
      121 ttcttgatcc cattgccgat aaattgacta tattcatact ttcgcttata ctgtatattt
      181 ttaagggttt tccattgtgg ttggtcattg tcatagtagt cagggatata ggccatattg
      241 ctgctggagc tattcttttc tgtacccatg atattcaggt gcgccccagc aatgccggga
      301 agtgtatgat ttttattttg gggatgacgg gttttatcta tattattaac ctgtcatatt
      361 cagtcatggg gataagcgta aagcacatct ttgtagcact taccactgcg cttattgttc
      421 tatcaacggt tgttcactgg aacatatatt ctaaaattta ctttacaagc aaaagtgttt
      481 cctctaaata aacagaatgc ttttttataa caaaaatcta tcccgccatt gttatagtat
      541 catcatgtta cttactgtat gtttactccc tttgtttccc caggatagca ttgtcatacc
      601 ggtaaaaatt ataaacagca acacatatgt ttctgaatat gacatacgaa cgatacttcc
      661 cctgcaatct acatttgata ttgtgttaca acggggaagg attgtcaata aagagaggta
      721 tgcactgtat catgttggtc tggcgatagc tctggtgaat gattctctgg taaggagtga
      781 ctatcctgtt atgaggcggc atggtgaggt aatgattccg ctggagattg ccttcaccat
      841 gctgacagct ttgcttcccg agtatgaagt agatataaaa gacaatgcaa tcaccctcaa
      901 aaagatacct gagtacgtaa aagaaaaaga aaaaaaaaca cccgtaacaa aagtatcaaa
      961 ggatgctatt ggttttattg tggttgatgc aggccatgga gggaaggacc ccggtgctat
     1021 aggtaaaggt ggcataaaag aaaagcacat aacgctgcag attgctaaag aggtaaagct
     1081 ggttttgcag caaaggttac ccggaataga ggtaatcttg acccggacta cagatagatt
     1141 tatagaattg ggtgagagga cagagatagc aaatacaatg ttaaaaaaag gtgttaatgg
     1201 gctttttata agtatacatt gcaatgcagc ggtggtgagt aagatatcag gttttgagac
     1261 atattacctt tcacaaaatc cttcaaatga agaggcacgt acaacctcgg tgcttgaaaa
     1321 caacgttatt atatttgaaa atcctgaaaa gcgcaagcga tacggtgatg ttgagatgat
     1381 tgaagcgctc atgattacaa cacagataca aaaagaaagt gcgatgcttg cagcatcagt
     1441 tcagagatat cttgacaaaa caatacatga atttaaatca aaaggagtaa aaaaagctga
     1501 cttctttgta ctgcgggggt gtcttatgcc gtccgtactt gttgaggtgg ggtatattac
     1561 caacccaaaa gaaaaaaaat atttaataga taaaaactat caaaaaaggc ttgcacaggg
     1621 gataacagca ggcattgttg catttataaa cgaatacaac aggaatatag ccaattattg
     1681 aggaatataa tgagattagg tgttaaaaat tttataacca gacacaagct tgatacgttg
     1741 cccggtatac taaaaatgaa actgttaacg tactatcaag caatagcagc cggaagtaat
     1801 aaacttatgg atctatgttt atactggatt tgtaatccac cgctggatac aaaaaaacgg
     1861 tatagagcat tgcttctggc aataagtgtt gtatgcatag ttgactactg cgcaatttca
     1921 gttcttgatg ataaaaattt atttgatatc tatccatcat tgcctgcgct ggatacccga
     1981 aaaaccattg atatttttgt tgctgatatt gatgcaaaat caatattaaa agaaaatagg
     2041 agggtgttac ttcctcagga tgatgaaggc aaagttaaca ggcttttaaa gctggtagcc
     2101 gaaggctctg aatttgagaa taccagggcg atagctcctg tagatataat cctgcgccat
     2161 atatggtttt atgataccac ctgtattatt gactgtgagc tgtccacaat agaaggacat
     2221 gtgccctcta tagctgattc tttccaagcg tttacaaaag ccttaaccca aacgatatgt
     2281 cacaatgtga aaggtgtaac gaaagttgaa gtgcttgaac atggtattta tggaaaaaat
     2341 ctctggtaat agaaataagc ctgtatacgt ggtatcacgg ccatacacct tcaaacacct
     2401 cttcagcagg gcctgctaaa tagacgtgat tatccttatt attccactct attgcaaggt
     2461 tgccgccttt gagatgtact tttaaccttc tatcagtttt ttctgttaac acaccggcaa
     2521 cggtaaccgc tgatgcgcct gtgccgcagg ctaacgtttc gccgcttccc cgctcccagg
     2581 ttcgctgtat aacctctttc ccggttacta tctgtacaaa ttcaacgttg gttcgttttg
     2641 ggaacaattc atggttctca atcataggac catatttttc aacagggaag tttttaacat
     2701 cctccacata gatgacaaca tgaggattac ccatagagac agcagtaatg gaaaacttta
     2761 cgccatcaac gtgaaggtct tcctctatta cggttccggg attaccaatc atgggtatct
     2821 tatctcttaa tagaatgggt tcgcccatat ctacggttac ggtgtgtacc tttccatttt
     2881 ttaaagtaca cctgacctgt tttattccag ctaacgtttc aatggctatt gttgtttttt
     2941 ttgttaaatt gtgatcgtac acatacttgg caaatccacg gatgccgttt ccacacatct
     3001 cggcttcact gccatctgca ttgaacatgc gcatggtaaa atcagcctta tctgatggca
     3061 gtatcaaaat aagtccgtca gaacctaccc cgaagttacg gttactcatg gctatagcta
     3121 atttttttgg atttttaacc gagtgtttta ttgcatttat gtagatataa tcattgccaa
     3181 taccgtgtaa ttttgtaaat ggtatcatag gtaacctcta tattctatgc actgtcattg
     3241 taatagatat aatacaaggc gttatgtgtc aagaaaatgt ttaaatagcg tggcgatagt
     3301 tattggtaaa taaaaaaata gttgttgcaa tatttagttg acttacattt cagatatttc
     3361 cgtaactggt aacgatgaaa ttagagtatt gaaaggcatg caacgatata atttaaacaa
     3421 caggaagttc agtaaaaagg ggtatgcagg tacaggcggc ttttttaccc gaatagttca
     3481 tttttttgaa gatgtgctgt atgctcttga actgcgattt ggcaatatcc gcccgataat
     3541 tattgggctt attgctggtg ccattattgc attttgtgcc attcttgtta tagattttta
     3601 taaggtacga gccctggctc actatacccc ctatcaaaca acaaagattt atgataaaaa
     3661 taatgttctt gtagcggagt ttttcaaaga aaaacgcgan nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnna gatttatgat aaaaataatg ttcttgtagc ggagtttttc
     3841 aaagaaaaac gcgaggtggt accacttaaa aaaattccac cacatctgat acaggcattt
     3901 attgcaatgg aggataacga gttttatgat cattttggta tcaatccaaa gggtattgtg
     3961 cgagcgtttt ttgtgaacat agtttcaggg cgtgtaaaac agggtgggag caccattacg
     4021 cagcagttat ccaagatatt gctcacctcg ggtgagcgct ctatctatcg caaggttaag
     4081 gaagctttta tctctattat gattgagatg ttctatacta aagatgagat tatggagatg
     4141 tatctcaatc aaatattttt agggcatggt gcttatggcg ttgagtcagc gtcaaaattt
     4201 tattttggca agcatgtctg ggagatcaat attgccgaaa gctccttgct tgcaacactg
     4261 ccttcagctc ccaatatact atcgcccata cgaaatccgc gtctttcaat ggagcgtcac
     4321 cgtatagcat tagctaaaat ggtggatatg ggttttataa ctgttgcaca ggcagagaag
     4381 gcataccttg atttctggcc tgattattta gcatatataa atgacctgcc acctacaatg
     4441 actacatggt catcacgtgt taacaaggca ccatggttta ctgagcatat acgccgtatt
     4501 ctggttaata agtttggtga aaaagcagta tacgaagatg ggctggctgt gtatacaacg
     4561 gttgatgtcg ataaacaaca atgtgcacaa agtttattga aagatgcagt ggaaaaacaa
     4621 actactgttt cgcgcgggct ggcatttgat aatgaagagt atatcaccaa tcgctatgct
     4681 gatgaagtca atttattgac cataatgttt gggttgcctg ctataaaaag tgccggtaca
     4741 gctcaaaaac gtacgtttaa ccggtatatg aaggatgggc tgcttcatga gatgcagatg
     4801 ataaatctta ttaccgggct tgatactatt gatgatgtat tgcaacagta ttttttaggc
     4861 agttatgatc agaaagagtt tatgaacgtt gagggttgtc ttgtctcaat aaatccaggc
     4921 aatggctata ttgaagcgat ggttggcggc agtgatttta ccagcaccaa tcagcttaac
     4981 cgtgttatgc aatccatgcg tcaggctggt tctgcaataa agcctctttt atatatagct
     5041 gctattgatt caaaaaaatt tactgcagcc actaccatta tggactcgcc tatattatat
     5101 cttgacatgg aaggcggcga ttggattcct gaaaattatg aaggcgaata ttatgggctg
     5161 gttactctac gcagggcttt atccttatcc atcaatgttg tttcggtgcg tatagccgat
     5221 acacttggta ttgataccgt gctcaacaat tatgcaaaac ttttaaagat ttcgcgcaat
     5281 gagatgaaaa accgcgttcc caggaatttt tctattgcgt tgggttcttt tgaggtttca
     5341 ccgcttgagc ttgcccgtgc ctatgccatc attgcaaatg gcggcaaaaa tgttattccg
     5401 tactccatac gctatgtgca ggataaaaca gggaagatca tctataatca ggaagaggag
     5461 ataatggcag agctggcaga gcaggaaaag gatggcagta tacagatagt ttctcctgat
     5521 gcggcacgga tagtggtaag cctgatgcag agtgttatat catcaggtac gggaagaggc
     5581 gctgccatag gcagacccgt tgccggcaaa acaggtacta ccagtaacta tcgagacgca
     5641 tggtttgttg ggtttacacc gcagcttgta acaggaatat ggatgggata tgatggcagg
     5701 ggattatcgc ttgggccagg acaatcgggg ggtgcagttg ttgctccggt gtggggacgg
     5761 tatatgagct gtgcactgca aaaggaaccg gtgcttgatt ttgcgccgta tggcaatatt
     5821 gctgtaacaa aagtgtgtgc tgagtcagga cttttgcctt cctattattg taaaaaggtg
     5881 atagatgaac tttttatacc aggaaccgaa ccgcaggaag aatgtgcaaa atgtgcaggt
     5941 gggatttcac tgccaacaac caaaatacca catatcaatg tatctgaaaa gcaaaaagag
     6001 cagataatga aaaaaatgaa aaggaaggat accgaggata tacttgactc aatagaaaat
     6061 actttgaaac aataaaggat taagggattg ctatggattt aaaaaatttt tatattgaag
     6121 cccaggatac agcactgttt gtcattgatg tacaggatag tttatttaac tcaatggaat
     6181 atgaaatgcg caggaaggtg gcaaagaatg tggcaatcct gatagagctt gcaaaacagt
     6241 ataacctccc tatagttgtg accgagcaat atcgtaaagg attaggttca accataaaag
     6301 aggttatgga taagctggaa ggagtagttc cttttgataa actgacattt gattgtatga
     6361 aagatgatag tatcagggaa caggtcgcag gccttaataa aaggaatctc atagtttgtg
     6421 gtattgaaac acatatctgt gttttgacaa catcgctttc gcttatgaaa gcaggatata
     6481 atgttgttat agcatctgat gctgtttgtt cacgacgaac ccatgaatgg gacatggcaa
     6541 tacagacatt gcgacaggca ggcgctgttg tgtatccaac tgagacaata gcgtttatga
     6601 tgctggaaaa agcaggaact gaaatcttta aaaaattgtc gccattgttt aaatagcttt
     6661 tttcaatgat taaggtttat cccacagtac atcataccca tcaaatacag gcccgtcaac
     6721 acatgcacgt ttgtatccag ctttagtggc aacagcacac ccggaacaca aacctattcc
     6781 gcatccaaaa tagcgctcca gtgaagcctg tatgggtatt ggcgatagtt cctgtgtaat
     6841 cctttgcatc ataatctcag ggccacaggt ataaatcata tcaaagctgt ttgttttaat
     6901 gatagctctg gctatatcaa ccggattgcc ttccacacca gcgctcccat catcagtgac
     6961 aacgatgagt tcgttggaaa tatttttata atcgtccagc atatagatag tgttttgtga
     7021 agttgcgcca tatatatagg ttaccgttga gttgtgttct cgcagcttct ttgcacaata
     7081 aaaaagagga gcattgccaa cgccacctcc gatcagcaaa gcttttttac cggtaactat
     7141 cgcaaaacct ttacctgaag ggccaatgca atcaattgct ccggggtgca gcgctgcaat
     7201 ctgcctggta gctttcccaa caacacgaac aacaatttga agattggtac ccgaatgatc
     7261 aaagattgag aatggacgtc gaataagcgg gtcagtatta ttggtaacct taatattaat
     7321 aaattgacct ggctgacttg cgagtacctc cgattgcaat gttagcaggt aatggtcact
     7381 tgcaacagat tttgtggata ctaatgtaaa cataattttt accctcaggt gctatagtac
     7441 aatacactat agcatgagtg caatttgcaa tactattttt taaagaattg tgtgaaatta
     7501 aacaatgctt ataactatta taagcatagc gattggtaat cgtacattac tttattgtaa
     7561 taaaaataaa aaatataatt attcagtatt tacattgctt gctggagtac ttctggtggt
     7621 ggtccccagt gatattggca gcaatgtatg ccattttcct tataaaaaga aaaggtgtat
     7681 gccctgcttt cataatagtt aggatagtta tcggtattca tctctt
//
LOCUS       DAOSOX010000110         7687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9511_length_7687_cov_7.858884, whole genome shotgun sequence.
ACCESSION   DAOSOX010000110 DAOSOX010000000
VERSION     DAOSOX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9511_length_7687_cov_7.858884"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLX22_08455"
     CDS             <1..228
                     /locus_tag="PLX22_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOT19967.1"
                     /translation="VPVAEKTSDGIKVQVGSVEHPMEEKHYIEWIQLIAGSKSYRQFL
                     KPGEKPVAIFKVDTASFAVREYCNLHGLWKA"
     gene            256..834
                     /locus_tag="PLX22_08460"
     CDS             256..834
                     /locus_tag="PLX22_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT19968.1"
                     /translation="MAKSVKGTKTEKNLLAAFAGESQARTRYTFFSSVAKKEGFEQIS
                     ALFLETAENEKEHAKRFFSYLEGGMVEIQAAYPAGVIGTTLENLKAAAAGENEEHTKL
                     YPESAKIAEEEGFPEIAFLFRKVAEVEKHHEQRYLDLAKNIEQGKVFKKEQKVQWKCR
                     NCGYIHEGNSAPEKCPTCQHPQSYFELLCDNY"
     gene            1055..1705
                     /locus_tag="PLX22_08465"
     CDS             1055..1705
                     /locus_tag="PLX22_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT19969.1"
                     /translation="MKRVEKNQQTRAKIIEAARTLFVQQSYSKTTIRQILKKTGITTG
                     SLYHFFKNKESILRQITADYLLDTDKIIRSFMKEYDPMVHYILIIYIQLKLIDSNPNL
                     ADIWQHTYSLWSITEMVCTKSAKWNKMMFGKYNENFSDDDWYARSMAINSLIHYITIE
                     KINRGEIPIKNRVHLLMHLVSLLFNTPFNQIESAIEKAEKIISKNSLQLYGVNLKL"
     gene            complement(1813..2826)
                     /locus_tag="PLX22_08470"
     CDS             complement(1813..2826)
                     /locus_tag="PLX22_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19970.1"
                     /translation="MSTKSKKFNIKQTFIIQVIASFVVISLLIILGGILVFNVMGKGY
                     SLFTAFGFIMTVYLAFITFQPAGILFNVRKEFVEGKVQLSDISSNETSSILVNPWIRT
                     IPLSATVAIVCTLIVGAIIYGFKWKPNAVVTIVLSMLYIVPHYLLTKRYIEKDIISFV
                     NGKSKLKNSSATGYFWGTYVLPNIVFQSIINYSLGNRGFHHEAMKLTQLFPQINNMVP
                     VNAVALDLSITFMFVCNFTFLAAISYTISGFYLGIITVHQKKDKPINGFLIFLIMLII
                     GLCFGICYSFFFKLSGIDNISIVAAMISKIMCVIIAVYAGSRMALGWTIKKITVNENK
                     YQS"
     gene            complement(2926..4755)
                     /locus_tag="PLX22_08475"
     CDS             complement(2926..4755)
                     /locus_tag="PLX22_08475"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HOT19971.1"
                     /translation="MEEKSVMQDQRTITFTEFITKFIPFTIRIPYILYHGIKGLNMIS
                     GKKNISWGLLLEKTAEKYPNNIAIKSHEGTLTYKELNEKANKFAHFLKSKNIVKGDTV
                     TLCIDTRPELLVLYCGCTKIGAVCSLINVNQRGDSLIHSFNLNKGKVVVIGEECYSFF
                     KEVESKVDIENSYLCFIRDSLTNQLKDKNDITELLSKMPETNLPDTRQITVHDPVAYI
                     YTSGTTGGLPKAAVITNIRLLSGTFWWGRIVTPITQKHTVYVPLPFFHTNAINIAWPP
                     AVYGGAAIAIRRKFSASSFVNDVKIFGATHFVYIGEVCRYLMAQPDIPGQDQTTLKYV
                     IGNGLRSDIWKSFKKRYGIKKIFEFYGAAEGVGVFTNIMNFEYCVGMCLTPWALVKYD
                     VVNDCIERDANGNAIKVRYGEIGLLIMQISKKTPFAGYTDKKKTEEKIVRDAFKKGDT
                     WFNTGDILRNMGYGQLQFVDRSGDTFRWKGENVAAMEVEKALDLFPGIQSSAVYGVSM
                     PSGDGKAGMAAIIKDKNIDLKALVKHLRSVLPKYAIPLFIRFVDDFERTATHKIKKTK
                     LKNEGYNPVNIKNDLYVLLPEKDSYVPLTESIYNQIMNNKYKF"
     gene            5068..6873
                     /locus_tag="PLX22_08480"
     CDS             5068..6873
                     /locus_tag="PLX22_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT19972.1"
                     /translation="MGTKFYSEENLKFLLQEVHDIDEIFSCDTFSDYNVESMNIILDT
                     AKNIASGMMYPYFSEMDKNPPEYTGSTIKVHPSVKPFLEKFAEGGWINASFPSDIGGQ
                     QMPFILFNACMFIFASANYSMSVYPGLTSGALALIYQFATKELYQIFAPPMIAGKWQG
                     TMALTEPEAGSSLSDVKTMAIPTDKGYYLIKGQKTFISAGDHDAVENIVHLMLARIKD
                     APQGVKGLSLFIVPKYRPDENGKLIFNDVNCAGCYHKMGYKGAPIAQLSMGEADDCRG
                     YLVGEPNKGLSYMFHMMNEARINVGLGAAAIAGAAYQTSLEYAKERRQGRKITEKNPS
                     VPQIPIIHHADVKRMLLKQKAIVEGSLSLIIQTGKLVDLINTSAGEEKEKYELFLDML
                     TPIVKTYPAEMGVVSTSLAIQCMGGYGYSSEFPVEQYMRDSRIHPIHEGTTGIQGMDL
                     LGRKVTMKNGATAKVYMTEIYKTIASAKQYPSLISYAEDLDKAAALWQEVTGFLIQFA
                     VKGDIERFLADATLFLEFSGLICIAWQWLLQGIAVQHSINNSSGSYSKDFLEGKMQTL
                     AYFFRYELTGIYALEKIIRGSDGLTVSMQENYFED"
     gene            complement(6920..>7687)
                     /locus_tag="PLX22_08485"
     CDS             complement(6920..>7687)
                     /locus_tag="PLX22_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOT19973.1"
                     /translation="LMDTNVLLKKNNYVATIILNRPPANSINIHVREELYAIVKELED
                     DNTIRVLIITGAGDKGFSAGMDVTDVANLDKGPDAIELFNYIDRYPKPVIAAINGYAL
                     GGGCELALACHFRLMTDMPKAFIGCPEVNLGITPGWGGIQRMTRLLGRSKALDCILFS
                     KRLTPQEALSIGLVDRVFPHEHFMEEVQNFADSLARRAPLAVSCILKGMITGIEKGID
                     EGLKVDRQCSAMLAKTEDAAEGFMAFAQKREPVFKGR"
BASE COUNT         2604 a         1394 c         1264 g         2425 t
ORIGIN      
        1 gtgccggttg cggaaaaaac atctgatgga ataaaagttc aggttgggag tgttgaacat
       61 cccatggagg aaaagcacta tattgaatgg atacagctta tagctggaag caagtcatat
      121 cgacagtttt taaaaccagg tgaaaaacct gtcgcaattt ttaaggttga tacagcatca
      181 tttgcagtca gggaatactg caatttacat ggtttgtgga aagcataatc taccattacg
      241 gaggataata aaacaatggc aaaaagtgta aagggaacaa aaactgaaaa aaatctatta
      301 gctgcatttg caggcgaatc gcaggcacgc acacgctaca cctttttttc atcagttgct
      361 aaaaaagaag gatttgaaca gatatccgca ttatttttag aaactgcaga aaatgaaaaa
      421 gagcatgcaa aacgtttttt tagttatctt gaaggtggca tggttgagat tcaggcagct
      481 tatcctgctg gagttattgg cacaactttg gaaaatctga aagctgctgc agccggtgaa
      541 aacgaagagc acactaaact ttatcctgaa tcagcaaaga ttgccgaaga agaaggattt
      601 cctgagattg catttctttt cagaaaagtt gctgaggttg aaaaacacca cgagcagcgc
      661 tatcttgacc tggctaaaaa tattgaacag ggcaaagtat ttaagaaaga acagaaagta
      721 caatggaaat gccgcaactg cggatatatt catgagggta actcagcacc ggaaaaatgc
      781 cctacatgtc agcatcctca aagctatttt gagttgctgt gtgataatta ttaatagtag
      841 tatggcatat cgatgattga aaatcccctt tataacaaag gggatttttt atactactct
      901 atactcattg aatgaccggc aatgggacaa ctatcataaa aaataattct tgtattatat
      961 attggatttc tttatgttta ctataattaa tgatatatca ttaattatag tatgtaatac
     1021 ttataattta gcatgaagtt ttaaaaatat taccatgaaa agagttgaaa agaatcaaca
     1081 aactagagca aaaataatag aagctgcacg aactttgttt gtacaacaga gttattcaaa
     1141 gactacaata cgtcaaatac taaaaaaaac aggtattaca acaggaagtt tatatcattt
     1201 tttcaaaaat aaagagagca ttctcaggca gattacagca gattaccttt tagacactga
     1261 taaaattata cgatctttta tgaaggaata tgaccccatg gttcactata tacttataat
     1321 ttatatccaa ctaaaattaa ttgacagtaa tccaaatctt gcagatatat ggcaacatac
     1381 ctatagcctg tggagtatta ctgaaatggt atgtactaaa agtgccaaat ggaataaaat
     1441 gatgtttgga aaatataatg aaaacttcag cgatgacgat tggtatgcac gatccatggc
     1501 aattaatagc ttaattcact atataaccat tgaaaaaata aatagaggcg aaataccaat
     1561 caagaatcgc gtgcatcttt taatgcacct tgtctcatta ctgttcaaca ctccttttaa
     1621 ccagatagaa tcagctattg aaaaggctga aaagattata agtaaaaata gtctgcaact
     1681 atacggcgta aatcttaaat tataaaatca agttaagtat agttactata tatcgaacac
     1741 agtgatacaa tgatattctt gttgcttatt cttgattata aagtgtaatt gcattaattc
     1801 ttctttgatt atttaagatt ggtatttatt ttcatttacc gtaatctttt tgatagtcca
     1861 tccaagggcc atccgcgacc ctgcatatac tgctataatc acacacatta tctttgaaat
     1921 cattgccgct actatggaaa tgttatcaat cccggataac ttgaaaaaga agctataaca
     1981 tattccaaaa cataatccaa taatcagcat tatcaaaaaa atcaaaaatc cattaattgg
     2041 tttatctttt ttctgatgaa cggtaatgat acccaaataa aatcctgaaa ttgtataaga
     2101 aattgcagca agaaaagtaa aattacacac aaacataaac gtaatcgata aatctaatgc
     2161 tactgcattg acaggcacca tattatttat ttgtgggaac aattgagtta atttcattgc
     2221 ttcatgatga aatcctctat tacctaaaga ataatttatt attgattgga aaacaatatt
     2281 aggcaataca taggtccccc agaagtatcc agtagctgat gaatttttta atttagattt
     2341 cccattaaca aaacttataa tatctttctc tatatatcgc tttgttaata aataatgtgg
     2401 tactatataa agcattgata atacgatagt aacaacagca ttgggtttcc atttaaaacc
     2461 gtaaataata gcaccaacaa taagagtgca aactattgca actgtagcag acaggggaat
     2521 tgttcttatc catggattaa caagaataga ggaagtttcg ttagaagata tatcagaaag
     2581 ttgtactttc ccttcaacaa actcttttct cacattaaat agtataccag caggctgaaa
     2641 ggttataaat gctaaatata cagtcataat aaatccaaaa gcagtaaaca aagagtagcc
     2701 tttacccatt acgttaaata ctagaatgcc accaagaata ataagtaaag aaataacaac
     2761 aaatgatgct attacctgta ttataaaagt ttgcttaata ttaaactttt tagatttagt
     2821 agacataaaa cttcctatta ttttatttat atttaacagc taaaattaat cacatatcta
     2881 ttcaaataac accatattat ttccttaatt gaaacagaac atcaattaaa acttatattt
     2941 attattcata atctggttat atattgattc agttaatggc acataactgt ccttttctgg
     3001 taaaagaaca tataaatcgt tctttatatt aacaggatta tacccttcat tttttaattt
     3061 agtcttttta atcttgtgcg tagctgttcg ttcaaaatca tcaacaaagc gaataaacag
     3121 tggtattgca tatttaggga gtactgatcg taaatgtttt accaatgctt taagatcaat
     3181 atttttatct tttattatcg ctgccattcc ggctttacca tcacctgaag gcatactgac
     3241 accataaact gctgatgact gaataccggg aaacaagtca agagcctttt caacttccat
     3301 agcagctaca ttttcacctt tccatctgaa agtatcacct gagcgatcaa caaactgtaa
     3361 ttgaccatag cccatgtttc tgagtatatc accggtatta aaccacgtat cccctttttt
     3421 aaacgcatct cgtacaattt tttcttctgt tttcttttta tcagtgtaac ctgcaaaagg
     3481 tgtttttttt gatatttgca tgataagcaa tccaatttca ccatatctaa ccttaattgc
     3541 attgccatta gcatcacgtt caatacagtc atttacaaca tcatacttca caagtgccca
     3601 tggtgtaagg cacataccaa cgcaatattc aaaattcata atattagtaa atacacccac
     3661 tccttcggca gctccataaa attcaaaaat ctttttaatg ccataacgtt ttttaaaact
     3721 cttccatata tcagatctaa gtccattccc aatgacatat tttaatgttg tttgatcctg
     3781 tccaggaata tctggttgtg ccatcagata cctgcatact tctcctatat agacaaaatg
     3841 tgttgcacca aatattttta catcattaac aaaactgctc gcagaaaatt tccttctgat
     3901 tgcgatagca gcaccaccat atacagcagg tggccatgca atattaatgg cattggtgtg
     3961 aaaaaatggt agaggaacat aaacagtgtg tttttgagta atgggcgtaa caattctgcc
     4021 ccaccaaaat gtcccggaca ataagcgtat atttgtaatt actgctgctt taggaagccc
     4081 accggttgta ccagaagtat agatatatgc aacaggatca tgaacagtaa tctgcctggt
     4141 atccggaaga tttgtttcag gcatttttga tagtagctct gtgatgtcat ttttgtcttt
     4201 caattgatta gttaacgaat cgcgaataaa acaaagatat gaattttcaa tatcaacttt
     4261 ggattctact tccttaaaaa aactataaca ttcctctcca attacaacaa cttttccttt
     4321 gtttaagtta aagctgtgaa taagggaatc ccctctttga ttgacattta ttaaagaaca
     4381 aactgctcca atttttgtac agccacaata taataccagt aattccggtc tggtatcgat
     4441 acataaagtg acagtatctc ctttaacaat attttttgat tttaaaaaat gagcgaattt
     4501 atttgccttt tcattcaact ctttatatgt aagcgttccc tcatgtgact ttattgctat
     4561 attattggga tatttttctg cagttttttc aagaagaagt ccccacgata tatttttttt
     4621 tccgcttatc atgtttaaac cttttattcc atgatacaaa atatacggta tacgtatcgt
     4681 gaaaggaata aactttgtaa taaactctgt aaaagttatt gtgcgttgat cttgcatcac
     4741 agatttttct tccatttgaa tgacctccta tatggtgaac aatagtttgt atttgttcat
     4801 acagaaaaac agaaaaaata attaactaca atactattcc acacaaaact tgtttaacaa
     4861 actaatacta atgtaatact gtaatataat ttgtcaatat aattttagaa catgttctat
     4921 tttattattg acataaattt atcgattaaa tcatttgata gggtatcttt aattaagtgt
     4981 aattaagttc atttttttaa aagtccataa cctttaaatt gccatttcac agttaaatca
     5041 actattacta ttgaaggaga attgagtatg ggaacaaaat tttacagcga agaaaatctt
     5101 aaatttttat tacaagaagt tcatgacatt gatgaaattt tttcttgcga tactttcagt
     5161 gattacaacg ttgaaagcat gaacatcatt ttagatacag caaaaaacat agcttcgggc
     5221 atgatgtatc catatttttc cgaaatggat aaaaaccctc cggaatacac aggttccacc
     5281 atcaaagttc atccttcagt aaaacccttt ttggaaaagt ttgcagaggg tggctggatt
     5341 aatgcatcgt ttccttcaga cattggggga caacaaatgc cttttatcct ttttaatgcc
     5401 tgtatgttca tctttgcctc cgcaaattat tcaatgagtg tttatcccgg tcttacatca
     5461 ggagcactcg cactcattta tcaatttgca acaaaggaac tttaccagat ttttgcaccg
     5521 cccatgatag ccggtaaatg gcagggcaca atggctttaa ccgaaccaga ggcaggaagc
     5581 tcattaagcg atgtaaaaac catggcaata cctactgata aaggatacta tctcataaaa
     5641 ggccagaaaa catttatatc tgctggtgat catgatgctg ttgaaaatat tgttcatctg
     5701 atgcttgcac gtattaagga tgcgccacaa ggagtaaagg gtttatcact tttcattgtt
     5761 ccaaaatata gaccggatga aaacggaaaa cttattttca atgatgtcaa ctgtgcgggg
     5821 tgttatcata aaatgggata taagggagcc cctatcgcac agctaagtat gggcgaagcg
     5881 gatgattgcc gtggatacct tgttggcgaa cccaataaag ggcttagcta tatgtttcat
     5941 atgatgaatg aagctagaat taatgtgggt ttaggagctg ctgccatagc cggagctgcc
     6001 taccagacat cgctggaata tgcaaaggaa cgccgtcagg gacgtaaaat tactgaaaag
     6061 aatccttctg ttcctcaaat acccatcata caccatgcag acgttaagag gatgctttta
     6121 aagcaaaaag ctatcgtaga gggttcactt tcattaataa tacaaacagg taaactggtg
     6181 gatctcatca ataccagtgc tggcgaagaa aaagaaaagt atgaactttt tttagatatg
     6241 ctcacaccca tagttaaaac ctatcctgca gagatgggcg tcgtttctac aagtttagct
     6301 attcagtgta tgggtggcta tggttattca agcgagtttc ctgtagaaca atacatgcgc
     6361 gactcacgca tacaccccat ccatgagggc acaacaggca tacagggaat ggaccttctt
     6421 ggccgcaaag tcacgatgaa aaacggtgct accgctaaag tatatatgac tgaaatttac
     6481 aaaactatcg cttcagcaaa acaataccct tcgcttatca gctatgctga agatttggat
     6541 aaagctgcag ctttatggca ggaggtaacc ggctttctca ttcaatttgc ggttaaaggt
     6601 gatattgaac gttttcttgc cgacgctact ctttttcttg aatttagcgg tctcatttgc
     6661 atagcatggc aatggttatt acagggaata gctgtgcaac atagtattaa caacagttca
     6721 ggaagctaca gtaaggattt tcttgaagga aagatgcaaa cacttgcata tttcttccgt
     6781 tatgaactta ctggtatcta cgctttagaa aaaatcatac gtggctctga tggacttact
     6841 gtatccatgc aagaaaatta ttttgaagat taattatacc ttgcattaca attagtaggc
     6901 gatagttact atctatttat tatctgcctt taaaaacagg ctcacgcttt tgagcaaaag
     6961 ccataaatcc ttctgctgca tcttctgttt ttgccagcat agctgagcat tgccgatcta
     7021 cttttaaacc ctcatcaata cctttctcta taccagttat catacccttc aggatacagc
     7081 ttaccgctaa tggtgcacgt cttgcaagag aatcagcaaa attttgtact tcttccataa
     7141 agtgttcatg tgggaacact cgatcaacaa gaccaattga taatgcttct tgaggagtta
     7201 atcgtttgct aaaaagtata caatcaagtg ctttgctacg tcctaacagg cgggtcatac
     7261 gttgaatacc tccccaacca ggagtaatac ctaaatttac ttccgggcaa ccaatgaatg
     7321 cttttggcat atctgtcatc aatctaaaat ggcatgctaa cgctaattca caaccgccac
     7381 ccaatgcata gccatttata gctgctatta ccggctttgg atatcgatca atataattaa
     7441 aaagttcaat tgcatcagga cctttatcca gatttgctac atcagttaca tccatacctg
     7501 cgctaaaacc cttatcgcct gcaccggtta ttatcagaac tctaatagta ttgtcatctt
     7561 ctaattcctt tactatagca tacaactctt ctctgacatg aatattgata gaatttgcag
     7621 gaggacggtt aagtattata gttgcaacat aattgttttt ctttagtaaa acatttgtat
     7681 ccattaa
//
LOCUS       DAOSOX010000111         7672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9534_length_7672_cov_8.000263, whole genome shotgun sequence.
ACCESSION   DAOSOX010000111 DAOSOX010000000
VERSION     DAOSOX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9534_length_7672_cov_8.000263"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLX22_08490"
     CDS             complement(<1..704)
                     /locus_tag="PLX22_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT19974.1"
                     /translation="MYQKKKANHYRRGNWYGKPYLFFGDYLFARYNTKIFKLSINADL
                     KCPNCDGTINSNGCIFCSEGSASPTANSTQSIHQQMQSAIDGFHRGLYAQKYIAYFQA
                     YTNTHASVAVLKQLYDTALQFDNVIGLMVGTRPDCLPDDVLDLIAQYKKENFELWLEI
                     GLQSVHDKSLQWLQRGHTYSQTVDAIHRAVMRGIDVCVHVILGIHGESWDEMMETANK
                     ISRLPVKGVKIHHLHV"
     gene            complement(673..2289)
                     /locus_tag="PLX22_08495"
     CDS             complement(673..2289)
                     /locus_tag="PLX22_08495"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT19975.1"
                     /translation="MIPCAVFAEQDIIATILNGEQSKERADEIIQIAGFIDELYSNLP
                     QRIKNGQKLVLFFDPAHGIDDDGKWEGEKTGRLSCTGLPEEFYSIAISRKLYTLLKKN
                     PYIQVASTKDYIDALEGNSDSYNNITFKETVELARNARAALVISEHLNNTASLFKAYG
                     LVNVKGIHVTYSNNTAYLTHIKEEHKGFLTLYNKFDTSGFSYKLASAVKGKLVANGLR
                     VNSWNFGTVADDRFSYFVNYPISVIFESGFISNPDDETMLRNPDYQQLIAKSHYESIL
                     ETIATTFGVDLSGFWGPRIIDTDFQKNLTLLKLSRIACNYISKNDTSKGLYVLQILLN
                     QCDPEKDTAVIENITDMKYRIERSQTLYTKALQLKKKGQIRKAKISMQKAISLISRSP
                     VFYEMRKAYRTEYGVLKETSPQKDYALQHPIPAKGVNPSPSSITTPVLIVLKNDITVS
                     EAIYNALRCDDAMLKKLTQQFASSKIKAGKKRFENPTKEGIYIVQLTPKGNIKSVKRV
                     TIVKLNPYKYQNQMYLKNSYCVSKEKSKSL"
     assembly_gap    2405..2504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2505..3023
                     /locus_tag="PLX22_08500"
     CDS             <2505..3023
                     /locus_tag="PLX22_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19976.1"
                     /translation="NYYVDGMFMINDTGDAKSQTGVALKLATSLRNDINLFFKPLFSQ
                     TTEHSNSINETDYRQIQGLFGAEYIYSLPQYPIAISFSAGVGISRTEIEPKDDTSGLE
                     ELKETGMAYGLWLGGQWLLTQWVAPFIELGYSKSLYNNDLDGASIGGMQIAIGVRFTI
                     WGKNKTFTSDYE"
     gene            complement(3029..4090)
                     /locus_tag="PLX22_08505"
     CDS             complement(3029..4090)
                     /locus_tag="PLX22_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19977.1"
                     /translation="MKKYVFILSFGIFILLSYNIFAKPIVAFGYLTNRSGNPNFNYME
                     VIFPNSFASAFEDSYDCTVIKPLKVNDLLEKKQLKLEKYYENYELPKIAKAIDCDLFI
                     FGYFVPLADNYIKIVLTLYASGSKHIFTFTNIGKMETEIFRIIDRITLVLCDFYGKDN
                     FFMFKTINKGSRIGLLTNLTPEELNSVYSVFMDSGYTVIALQGNELDSYYAHDETIMN
                     YFQYISTNDNSYDFITDWTMFKIATGAWQGKKNSQWYEDMRKFYEIYDIHYKIFKNTI
                     LEKYKTIYRGCDYVLVVVFNDARSSCWVRAIDINAKQLVFMHPNISAAGMGKDIQKTT
                     RELIQVMQTPTSLPQKAKK"
     gene            complement(4102..4665)
                     /locus_tag="PLX22_08510"
     CDS             complement(4102..4665)
                     /locus_tag="PLX22_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19978.1"
                     /translation="MKRIMVCILVSYIAFVSCAAKKGVVARKVYVTPGSRIIVIANHS
                     NNIKNVIMAQFMNKNFSVKAFNASDLYTIDDIYDIKDFKKVAYMQPLKNDEMISFEKA
                     VENIYKLHIYNYELQKAEALTDMRTKYGVQFLVLMELKDWQKVSWARAIDLTNMEVVW
                     VENYPAQYKDTLESIIDHFIVSMSGQK"
     gene            complement(4764..5750)
                     /locus_tag="PLX22_08515"
     CDS             complement(4764..5750)
                     /locus_tag="PLX22_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19979.1"
                     /translation="MDKNDVKNDVKNDVKNDLVSFDENPYSHEIKKPEDIVRLFYEYN
                     VPILPVISKRGILFGILKKDDVIAELSDIARSSIRVDEFVQKLAKKMTMDELIPLVVN
                     VNDIVAIDLFGEVQGKWSRLDLFMAAEGHFGKKDKKGQDSEIQPKDTAIEWLIYLVLE
                     HIPRALYAVNSNGKTIFFNGHFEEVYLNTYPDSSDVDVAQVETLLMDTTRNDIFTSYV
                     HKRQYFFNKEMKTYYERVPLKDENKTVGYLFLFERDITKQDLLIPDIISESDGIDELL
                     SRAHRAILVKALVQSDYELATTATMLHITEQDLKKRIKNFNITINKPLEYDK"
     gene            complement(5756..6544)
                     /gene="dapB"
                     /locus_tag="PLX22_08520"
     CDS             complement(5756..6544)
                     /gene="dapB"
                     /locus_tag="PLX22_08520"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT19980.1"
                     /translation="MKVALCGVTGRMGKTILSVLLEKGHELSQALDADSCPMLGQDAG
                     AFIATKTGIAIQSLSSVSLAADVAIDFSTPSATEVLLQKAVASSIPVVIGTTGFSETQ
                     RIKIQRAAEFIPIVFSPNMSVGVNVLFKLTQIASSILQEGYDVEIFEAHHRLKKDSPS
                     GTAKKLIDIVLENRKDLEQAKLMYGREGIVGERTNNEIGVMVLRGGDIVGEHTVYFVA
                     NGERIELTHRATSRENFARGAVKAAEFVINKTPGLYTMFDVLGL"
     gene            complement(6541..7419)
                     /gene="dapA"
                     /locus_tag="PLX22_08525"
     CDS             complement(6541..7419)
                     /gene="dapA"
                     /locus_tag="PLX22_08525"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT19981.1"
                     /translation="MFEGVFTALVTPFKNGKIDYDSVAAIIEHQIAGGVDGVVPMGTT
                     GESPTVSYEEHEEFIVRVVTLVNKRVKVIAGTGSNSTDEAIYLSKAAQDAGVDGVLLV
                     NPYYNKPPQRGLIAHFESVAQSINIPVILYNIPGRTGINFLPESVAQLIQRVPNIVAM
                     KEASGDINQMMNLVELCGNTITLLSGDDNLLLPVLAIGGKGIISVISNLLPNDVKAVV
                     TNFNNGNLEEAKRLFYKLLPLCRAMFYETNPIPIKAAMAMAGFCSSEMRLPLVPLSAD
                     NHERLKKALLAYGVKL"
     gene            complement(7460..7594)
                     /locus_tag="PLX22_08530"
     CDS             complement(7460..7594)
                     /locus_tag="PLX22_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19982.1"
                     /translation="MTRKVNNNYSLWAIVTGNPWFSIKIFQLDKIFLLLTPMYYIGNI
                     "
BASE COUNT         2273 a         1553 c         1298 g         2448 t
ORIGIN      
        1 ataacatgga gatgatgaat ctttacccct tttaccggaa gcctgcttat tttattggcg
       61 gtttccatca tttcatccca gctttcgccg tgtataccca gaataacatg tacgcataca
      121 tcaataccac gcataacagc acggtgtatg gcatcaaccg tctggctata cgtatgcccg
      181 cgttgcaacc actgcaggct tttatcatgt acactttgca aacctatctc aagccacagc
      241 tcaaaatttt cttttttgta ctgtgcaatg agatcaagca catcatcagg aaggcaatcc
      301 gggcgtgtcc ctaccataag cccaatgaca ttgtcaaact gcaatgctgt atcataaagc
      361 tgttttaaca ctgccaccga tgcatgggta ttggtatatg cctgaaaata agcaatatac
      421 ttttgagcat acaagccacg atgaaagcca tcaatggcag attgcatttg ctgatgtatt
      481 gattgtgtgc tatttgctgt aggtgatgct gaaccttccg aacaaaaaat acacccatta
      541 ctgttgatag taccatcaca gttggggcat tttaaatcag catttatgga aagtttaaaa
      601 atttttgtat tatacctggc aaataaataa tccccaaaaa aaaggtacgg cttgccatac
      661 cagttcccac gcctataatg atttgctttt ttcttttgat acacaatatg aattctttaa
      721 atacatctga ttttgatatt tatacggatt aagtttaaca attgttacac gctttacact
      781 tttaatgttc cctttagggg ttaactgtac tatgtatata ccctcttttg tgggattttc
      841 aaatcttttt ttgcctgctt ttatttttga agaagcaaac tgctgcgtta gcttttttag
      901 catagcatca tcgcatctca gggcattgta gattgcttcg ctaactgtta tatcgttttt
      961 tagtacaatg agtactggtg tcgtaatgga tgatggtgac ggattaaccc ccttagcagg
     1021 tataggatgt tgcaaagcat aatctttctg gggagatgtt tcttttaaaa caccatactc
     1081 tgttctgtac gctttcctca tttcataaaa aaccggtgag cggcttatta aacttatagc
     1141 tttttgcatg ctaatttttg ccttcctgat ctggcctttc tttttcaatt gcaaagcttt
     1201 agtatataat gtttgtgaac gttcaatgcg atacttcata tcggtaatgt tttcaatcac
     1261 agccgtatct ttttcaggat cgcactgatt taataaaatc tgcaaaacat ataatccttt
     1321 tgaagtatca ttttttgata tgtaattaca ggcaatccgt gacagtttaa gcaacgtaag
     1381 gtttttttga aaatcagtgt caataattct gggaccccag aatccagata aatcaacacc
     1441 aaaggttgtg gcgatagttt ccagtatcga ttcatagtgg gattttgcaa tcagttgctg
     1501 atagtcagga ttgcgcagca ttgtttcatc gtcaggattt gatataaatc cggactcaaa
     1561 gattactgat ataggatagt tgacaaaata cgaaaatcgg tcatcggcta ctgtaccaaa
     1621 attccagcta ttgacacgca aaccatttgc tacgagctta cccttaacgg cgcttgccag
     1681 cttatatgaa aatccgcttg tatcaaattt attatataac gttaaaaatc ctttatgttc
     1741 ctctttaata tgggtaagat atgctgtgtt attactatag gttacatgta ttcctttcac
     1801 attgacaagc ccatacgctt taaacaatga tgctgtatta ttaagatgtt ctgaaattac
     1861 cagtgctgct ctggcatttc gggctaattc aactgtctcc ttgaaagtga tattattata
     1921 actatcagag ttaccctcta atgcatcgat gtaatccttt gtacttgcta cctgtatata
     1981 tggatttttt tttaaaagtg tataaagctt ccgtgatata gctattgaat agaactcttc
     2041 gggcaatcct gtacagctta aacgtcctgt tttctcacct tcccatttac catcatcgtc
     2101 aatcccatgt gctggatcaa aaaatagtac cagcttctgg ccatttttaa tcctttgtgg
     2161 aagattgctg tataattcat caataaatcc tgctatttga attatttcat cagcacgttc
     2221 ctttgattgc tctccattta aaatggtagc gataatgtct tgctcagcaa atactgcgca
     2281 gggaatcatc aagcaaatca ataatataca tagcaacaca ctttttttca taatcaataa
     2341 ctatcgcttt atatattgat acattataca gttataatgt cgaataaatt tccattcagg
     2401 ttaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaactac tatgttgacg
     2521 gcatgttcat gattaatgat acaggcgatg caaagagtca aacaggggtg gctttaaaat
     2581 tagcgaccag cttaaggaat gatattaatc tattctttaa accattgttt agccagacaa
     2641 ctgaacattc caatagtatc aatgaaactg attaccggca gattcaaggg ctttttggag
     2701 ctgaatatat atactcgtta cctcaatatc caattgcaat aagttttagc gccggggtag
     2761 gcatttcaag aacagagata gaacctaaag acgatacttc aggattagaa gaattaaaag
     2821 aaactggtat ggcgtatgga ttatggttag gcgggcaatg gttattaaca cagtgggtag
     2881 ctccttttat tgagcttggt tactcaaaat cactctataa taacgatctt gatggtgcca
     2941 gcataggcgg gatgcagata gcgataggtg ttcgttttac tatatgggga aaaaataaaa
     3001 cgtttaccag cgattatgag taatctgatt atttctttgc tttctgcggt aatgacgttg
     3061 gtgtttgcat tacctgaata agctccctgg ttgttttttg aatatccttg cccataccgg
     3121 cagcactgat attgggatgc ataaacacca gctgctttgc attaatatca atagcccgca
     3181 cccagcaact tgaacgtgca tcattaaata ccactaccaa tacatagtca cagccacgat
     3241 agatggtttt atatttttcc agtatggtat tcttaaatat tttataatga atatcatata
     3301 tttcatagaa cttccgcatg tcctcatacc actggctatt ctttttcccc tgccatgcac
     3361 cggtagcaat cttaaacatg gtccaatcag taataaaatc atacgagtta tcattagtac
     3421 ttatatattg aaaatagttc ataatcgttt catcatgggc atagtagcta tcaagctcat
     3481 tgccctggag agcaataacc gtatagcctg aatccataaa tactgaataa acgctgttaa
     3541 gctcttccgg tgtaaggttt gtcagcaaac caatacgact gcccttattg atagttttaa
     3601 acataaaaaa attgtccttg ccataaaagt cacagaggac cagggtaata cggtcaatga
     3661 ttctaaatat ctcggtttcc attttaccaa tattggtaaa agtaaaaata tgctttgaac
     3721 cacttgcata gagggtcagt acgattttga tgtaattatc cgccaatggc acaaaatagc
     3781 caaatataaa taaatcgcaa tctattgctt ttgcaatttt ggggagctca tagttttcat
     3841 agtatttttc taactttagc tgcttttttt caagtaaatc atttacctta agaggcttta
     3901 tcacagtaca gtcataagaa tcttcaaatg cagatgcaaa tgaattggga aagattacct
     3961 ccatataatt aaaattggga ttaccacttc ggtttgtcag gtagccaaaa gcaacaatgg
     4021 gtttcgcaaa tatattatat gacagtaaaa tgaaaatacc aaaagacaat ataaaaacat
     4081 attttttcat attctatcag gttacttttg cccgctcatg ctgacgatga aatgatcaat
     4141 aattgattcc agagtatcct tatactgtgc aggatagttc tcaacccaca ccacttccat
     4201 attggttaaa tcaatggcac gcgcccatga taccttctgc cagtctttca gctccataag
     4261 gacaagaaac tgtacaccat attttgtacg catgtcggtt aaggcttctg ccttttggag
     4321 ttcatagttg tagatatgta atttatagat gttttctacc gctttttcaa aggatatcat
     4381 ctcatcattc tttagaggct gcatatatgc tactttttta aagtctttaa tatcatatat
     4441 atcgtcaatg gtgtatagat ccgatgcatt gaatgcttta accgaaaaat ttttattcat
     4501 aaactgcgcc atgataacat tcttaatatt gttggaatga ttggcaatga caattatccg
     4561 tgagcccggc gttacataca ccttacgagc aaccacacct ttttttgcag cacaggatac
     4621 aaaagcaata tatgatacca gtatgcacac cattatcctt ttcattatat actaccccat
     4681 acaataaatt ttataataat ctattattaa ctattaatca atttgattat agaatagcat
     4741 ataacaaagt caagataatt ttattattta tcgtattcca gaggtttgtt gatagttata
     4801 ttgaaatttt ttattctctt ttttaaatcc tgctcggtaa tatggagcat agtggcagtt
     4861 gtagcaagtt cataatctga ttgtaccagt gcctttacca gtatagctct atgcgcacgg
     4921 gaaagcaact cgtcaatacc atcgctttct gagataatat caggtataag caaatcctgt
     4981 ttggtgatat ctctttcaaa taaaaagagg tatcccacgg ttttgttttc gtcttttaac
     5041 ggtactcgtt cataatatgt tttcatttcc ttattaaaaa aatactgtcg cttatgcaca
     5101 tatgaggtaa aaatatcatt tctggttgta tccataagca gtgtttcaac ctgagcaacg
     5161 tcaacatctg aagaatcggg atatgtattc agataaacct cttcaaaatg tccgttaaaa
     5221 aaaattgttt tcccattact attgacagca tacagcgccc gcggtatatg ctcaagtact
     5281 aaatagataa gccattcaat agcagtgtct ttaggttgga tttcgctgtc ctgtcctttt
     5341 ttatcttttt ttccaaagtg tccctcggct gccataaaaa gatccagcct gctccacttc
     5401 ccctgcacct cgccaaatag atcaatggca acaatatcgt taacatttac cacaagagga
     5461 atgagttcat ccattgtcat cttctttgcc agcttctgaa caaactcatc tacccttatt
     5521 gagcttcgtg ctatatcgct tagctcggca ataacatcat ctttttttaa tataccaaaa
     5581 agaattcccc gttttgaaat gacaggtaaa atggggacat tatattcata gaataaacgg
     5641 acaatatcct ccggcttttt tatttcatgg gaataaggat tttcatcaaa tgaaaccaga
     5701 tcatttttaa catcattttt aacatcattt ttaacatcat ttttatccat actgttcata
     5761 atcccaatac atcaaacatt gtataaagac ctggcgtttt attaataaca aattcagctg
     5821 cttttactgc accacgtgca aaattttcac ggctggtagc cctgtgggta agctcaatcc
     5881 gctcgccatt tgctacaaaa tatacagtat gttcgccaac gatatcaccc ccacgcaaca
     5941 ccataacccc aatttcattg tttgttcttt ccccaacaat gccttccctt ccatacataa
     6001 gctttgcctg ctcaaggtcc ttccggtttt ccaatacaat atcaatgagt ttttttgctg
     6061 tcccggatgg ggaatctttc tttaaacgat gatgtgcttc aaatatctca acatcatatc
     6121 cttcctgcaa tattgacgat gcaatctggg taagtttaaa taatacatta acaccaaccg
     6181 acatgttggg tgaaaataca atgggtataa attcggctgc tctttgtatc tttatcctct
     6241 gtgtttcaga aaaaccggta gtgccaataa caacagggat tgacgatgca accgcttttt
     6301 gcaataacac ttcagttgca gaaggagtac taaaatcaat agcaacatca gcggcaaggc
     6361 ttacactgga taacgattgt attgcaatgc cagtctttgt tgcaatgaaa gcaccggcat
     6421 cctgtccaag cataggacag ctatcggcat caagagcctg ggaaagctca tgtccctttt
     6481 caagcaatac tgacagtatg gttttcccca tcctgccggt aacaccgcac agtgctactt
     6541 tcatagctta accccatacg ccagcaatgc tttttttaac ctttcatggt tatcagcgga
     6601 caggggtact aacggcaacc gcatttcaga agagcaaaag cctgccattg ccatagcagc
     6661 ttttataggt atagggttgg tttcatagaa catagcgcgg catagcggta ataatttata
     6721 gaaaagcctc tttgcttctt caagattgcc gttgttaaaa ttagtgacca cagccttcac
     6781 atcgtttggt aaaaggtttg aaattaccga tataatacct ttcccgccaa tagccagcac
     6841 aggcagcagc aaattgtcat cacccgataa cagggtaatg gtattgccac atagctcaac
     6901 aaggttcatc atctgattaa tatcacccga agcctctttc atggcaacga tattgggtac
     6961 acgctgtatg agctgggcaa cactttcagg aaggaaattt attccggttc tgccaggaat
     7021 attatacaga ataacgggaa tattaattga ttgtgcaaca ctctcaaaat gagctatcag
     7081 tccacgctgt ggtggtttat tgtaataggg atttaccagt aacacgccat caacccccgc
     7141 atcctgtgct gcctttgaaa ggtatatagc ctcatcggtt gaatttgaac cggtaccagc
     7201 tataaccttt acgcgtttat tgaccagtgt aacaacacgg acaataaatt cctcatgctc
     7261 ttcatagctt accgtaggag actcgccggt agtacccata ggcacaacac catccacgcc
     7321 gccagcaatt tgatgctcaa tgattgctgc tactgaatca tagtcaatct taccattttt
     7381 aaatggggtc accaacgcag taaacacacc ttcaaacatg cacgcctcac tgtagtttac
     7441 ataaaaattg caagcctatt catatattgc ctatatagta cataggtgtt aaaagcaaga
     7501 atattttgtc aagctggaat atttttatac taaaccatgg attaccggta actatcgccc
     7561 acaggctata gttattgttt acctttcttg tcatcgttat ttcctgtggc aaatttttcc
     7621 ggaggtttta ctaccgaagc ccgcaacagg tttaaacccc tatgatgcat ac
//
LOCUS       DAOSOX010000112         7623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9613_length_7623_cov_8.111258, whole genome shotgun sequence.
ACCESSION   DAOSOX010000112 DAOSOX010000000
VERSION     DAOSOX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_7623_cov_8.111258"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1303
                     /locus_tag="PLX22_08535"
     CDS             233..1303
                     /locus_tag="PLX22_08535"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT19983.1"
                     /translation="MNSILTKIQEAINLLPATLMQLKQEGRKIIMTQCDILPYEVFRA
                     FGYSVIKMPRWVANAFIMGDSSLENDAVAFCTIADMIIVPKQCTIVPNAILSNRIFLI
                     DGINGFAEDAAVAIHSMLSMILRTVGTTQEYPDDEELKEAVCLYEEIRKIMRTVSSFH
                     AHKLTLIQLQLICDAAFSLLPDESLPLLQQIIVALESMDVPVPDNGEEKIKTLLYSDG
                     TNWEMFDECKKHDLTIVEDDACNVRRQFDISCNVSSTNLYYELLMAYTFKAWCPAMRS
                     APERFELIYKALPNYDIGLVIFLPSVLTIRNNHIDYLYEQCLLQGSNAIVCTEEEAIV
                     QIQRYITALKQRKHIAITIDLL"
     gene            complement(1352..3307)
                     /locus_tag="PLX22_08540"
     CDS             complement(1352..3307)
                     /locus_tag="PLX22_08540"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOT19984.1"
                     /translation="MKKLTYVCIALVLFSGCYKKSVVLNPNDIHYIISTFLNEHVQYH
                     EFNNTISDRTLNNLLSSIDPGKYYFYKNDVDGFTKRYSTLIDDLSKKEDFSFLDDIFG
                     TYRKRVAEVNDMINQLLKQQYTFTEDETINLDSDKISYAIDKKELKERWRKNIKLQLL
                     NYQTTDNLKEEQAKEKLKRKFFINNKRIQELDKAKQFSIFLNAFSMALDPHTNYLTQE
                     EHEDFMISNNLKLEGIGVQLRSEDGYTIVDRIIPGGAADKLSKELQLQPNDRIVAVAQ
                     DNDEPVDVVDMDLRDVVKMIRGKKGTLVKLTIIRINPTSNEQKRMVIPIVREEINLED
                     SAVKSEVHQSKNGMVIGYIKIPSFYVDITKQGFDQGRSSTADTIMQINNLMKKNVACI
                     VIDLRGNPGGALSEALKLAGLFVDAGPIMQVYDSSDTIQSLDDPIPGVYYSGPLVVLI
                     DKFSASASEIFAGAIRDYRRGIIIGTDATFGKGTVQSYKELYQKKGAIKITNAIFYQP
                     SGTSNQLNGIKPDITVPDITTIWDIGENKLKYALQWKPIPPAAYTPYRNYIPGEVVAL
                     LRNKSDSRLQNDAHYAKLLKEIQELKQKLQSKEISLKDESTIEQRKKDLEETIRKNKT
                     ENLIDLENDPFLKEALNITVDYIKVLQ"
     gene            3508..4935
                     /gene="gatA"
                     /locus_tag="PLX22_08545"
     CDS             3508..4935
                     /gene="gatA"
                     /locus_tag="PLX22_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOT19985.1"
                     /translation="MIGKSMQELIALLESNAVTSVEIVQAFLQEMNHKQELNAYITVL
                     DDDALQQAQQSDKRRKAGKPLSQFDGIPIAIKDNICTQGILTTCASGILKNFIPPYNA
                     TVYQKLLNAGFIVLGKTNMDEFAMGSTTETSFFGPTKNPVDITKVPGGSSGGSAAAVK
                     ACCAPVALGSDTGGSIRQPASLCGVVGIKPTYGRVSRYGLVAFASSLDQIGTFANTVK
                     DAADVLAVISGVDRCDATSIAMPVDFGSDTITGTIKGLRIGVPREYFNGVDNQIVQAV
                     ETVIKQLELKGAIVHTISLQYTDYAVPIYYLIATAEASSNLGRYDGVRYGYRSPNIAK
                     LKDLYTKTRQEGFGKEVKRRIILGTFALSSGYYDAYYLKALKGRRLIIDDFNKAFEEC
                     DCIVAPVTTTTAFGIGEKIADPLALYMADILTISANLAGIPGMSVPVAEDTHGLPIGV
                     QLMARHFNEMVMLNVAQAIEDSIGR"
     gene            4913..>5511
                     /locus_tag="PLX22_08550"
     CDS             4913..>5511
                     /locus_tag="PLX22_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19986.1"
                     /translation="MKIALVVSIAVHCACAYRYTALRIGTVVTVPEHESVAPEQLTAT
                     VYYTAYVVTMTKGGDEAKKNNAAVEALLKGKIDDALVVLESIVAYNEPAVLNNYGVAL
                     LINGNSDSGLQYICRAAVLQPDNAYFKKNYLYLHECKNDIVIFFLAIVCCIGCLHEPV
                     DSDYYDDLSLVKGGEKPTPPVDLSLTVADHEVTLQFYTD"
     assembly_gap    5512..5611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5772..6335
                     /locus_tag="PLX22_08555"
     CDS             5772..6335
                     /locus_tag="PLX22_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19987.1"
                     /translation="MNYRIAMVLFFIFYTPALCGQNKPFTFVPAEQPLSLYKALAYDI
                     VPGGGHIYTGHYEYAAVFATLKISGVYAMYYYYNYWHYRGSLYRSAKKANAAIDPEHD
                     LQFKDPEGGYKTVEEFRQAYDRAAHYFTLSVAANVAIYVTSWLMTLYHVQQINEERQP
                     AYIGFAAHVLLDADAYGTVYAGYTVKW"
     gene            complement(6441..6515)
                     /locus_tag="PLX22_08560"
     tRNA            complement(6441..6515)
                     /locus_tag="PLX22_08560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6478..6480),aa:Asp,seq:gtc)
     gene            6703..7623
                     /locus_tag="PLX22_08565"
     CDS             6703..7623
                     /locus_tag="PLX22_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT19988.1"
                     /translation="MSLTIFDDIYHEIRKRNTSGGFKAVPYSDEFIKYAAGYYGLSME
                     LIKNIIQILINSHKIFSIEIIAEDKVRDIAHVEGYVVCDLVILRKLKSFYQSELIIQY
                     ENEFHKRLMVHQVVKEIFPMLHSLNNTDIGKVANLAIMLEELERFMEKHYEEFTEEWK
                     AKRLAYEISLSNIDTQLERKVEKQKVAGRSSSPGGLKDRRKKRVVDAPQYNEFIDKSK
                     SYPLERIFQIYGIDFFLRVQLRNYKFDYLKELVGTKKIKRKSDLLLLKNMLNAVLHNR
                     NGDHNLSQYEDAIYELMQTVNHHLFVERDF"
BASE COUNT         2316 a         1318 c         1554 g         2335 t
ORIGIN      
        1 gttcaatatt ttctgcttca ttaaacacag gcaatacaat ggaaaggtat ggtttctttg
       61 tggttcttga tttcatatat tgctctccgg aatcaattgc ataatggtat ccctttattg
      121 tatacatata atctatcgca agtaaaataa taaattgtaa acaatatttt tattgaaaaa
      181 ttaatcatca tgttattgct gaattaaaaa aggggtgatg gcatatatgg atatgaatag
      241 tatacttaca aaaatacagg aagctataaa tctacttccc gcaacattaa tgcagttgaa
      301 acaggaagga aggaaaatta taatgacaca atgcgatatc ctcccctatg aggtttttag
      361 agcttttgga tatagtgtga ttaaaatgcc gcgctgggtt gccaatgctt ttattatggg
      421 cgatagttct ctggaaaacg atgcagtagc attttgcact attgctgata tgattattgt
      481 ccccaaacaa tgtaccattg tccccaatgc gatattgagc aatagaatat ttttaataga
      541 tggtatcaat ggttttgccg aagatgcagc agtagccatc cattccatgc tttcaatgat
      601 tttgcgtact gtggggacta cacaggaata cccggatgat gaagaattaa aagaggctgt
      661 ttgcctatac gaagagatac ggaagattat gcgcaccgta tcgtcgttcc atgcacataa
      721 actaacttta attcagttac agcttatttg cgatgcagca ttttcgttgc ttcccgatga
      781 aagcttacca ttattacagc agatcattgt tgctcttgaa tcgatggatg ttccagtacc
      841 tgacaatgga gaagaaaaga ttaagacact gctctatagt gatggcacta actgggaaat
      901 gtttgatgaa tgtaaaaaac atgatttaac aatagttgag gatgatgcgt gcaatgtcag
      961 gaggcagttt gatatttcat gtaatgtctc ttcaaccaat ttgtactatg agctgctcat
     1021 ggcatatacc ttcaaagcat ggtgccccgc aatgcgcagt gcaccagagc ggtttgaatt
     1081 gatatacaaa gctttaccca attatgacat tgggctggtt atctttttac catcggtgct
     1141 taccatccga aataaccaca ttgattatct ctatgagcaa tgcttgctgc agggaagcaa
     1201 tgcaatagta tgtactgaag aagaagccat tgtacagata cagcgatata taaccgcact
     1261 aaagcaacga aaacatattg caataacgat tgatttgctg tgatataagc tatcgtgaca
     1321 cagagagcgt ttgcagcact ataaactgtc gctattgtaa aactttaata taatccacag
     1381 taatattcaa tgcctctttc agaaagggat cgttttctaa atcaataaga ttttctgttt
     1441 tatttttacg gattgtctcc tcaaggtctt ttttacgctg ctcgatggtg ctttcatctt
     1501 ttaaacttat ctctttggat tgcaactttt gttttagttc ctgaatctct ttcaataatt
     1561 tggcataatg agcatcattt tgaagtctac tatcagattt attgcgaagc aatgctacta
     1621 cttcaccagg gatatagttt cggtacggtg tatatgcagc aggaggtatt ggcttccact
     1681 gcaatgcata tttgagctta ttctcaccta tatcccatat tgttgtgata tccggtacgg
     1741 tgatgtcagg ttttatccca ttgagttgat tggatgtccc tgatggttga taaaaaatgg
     1801 cattggttat tttgattgcg cctttcttct gatatagttc tttatatgat tgcactgtac
     1861 ctttaccaaa ggttgcgtca gtaccaataa ttatcccgcg ccggtaatca cggatagcgc
     1921 ctgcaaaaat ttctgatgcg ctggcactga atttatcaat gagtactact aatggtccac
     1981 tatagtatac accgggtatt ggatcatcca gagattgtat agtatcagaa ctatcgtaca
     2041 cctgcataat aggtccggca tcaacaaata aaccagctaa ttttaatgct tcactcaacg
     2101 cacctccggg attgccacga aggtcaataa cgatgcaggc aacatttttt ttcataagat
     2161 tgttaatctg catgatggtg tcagcagtgg aacttctacc ctgatcaaat ccctgtttgg
     2221 taatatccac ataaaatgat ggtattttaa tatatccaat taccatgcca tttttagact
     2281 ggtgcacctc ggatttgacg gcactatctt ctaaattaat ttcctcacgc actataggaa
     2341 taaccatgcg cttttgctca ttgctggtag ggttaatccg tattatagta agttttacca
     2401 gcgtgccctt tttcccgcga atcatcttca caacatcgcg cagatccata tccacaacat
     2461 caaccggttc atcattatcc tgagcaacgg caacaatgcg atcattgggc tgtagctgta
     2521 attctttgga aagcttatcg gcagcacccc cggggattat acgatcaaca atggtatagc
     2581 catcctcaga acgcaactgc acgccaatac cttcaagttt tagattgttg gatatcatga
     2641 aatcttcgtg ttcttcctgt gttagatagt tggtatgagg atcaagcgcc atagaaaagg
     2701 catttaaaaa aattgagaat tgttttgcct tatccaattc ctgaatacgt ttgttattga
     2761 taaaaaattt tcttttcaat ttttcttttg cctgttcttc cttcagatta tcagtagttt
     2821 gataatttaa aagttgcagc ttgatatttt tacgccatcg ttcttttagt tcctttttgt
     2881 cgattgcata cgatatttta tcactgtcta aattgatggt ttcatcctcg gtaaaggtgt
     2941 attgctgctt cagcaattga ttaatcatgt cattaacctc ggcaacgcgt ttgcgatagg
     3001 tgccaaaaat gtcatcaaga aaagaaaaat cttctttttt tgaaagatcg tcaatgagtg
     3061 tggaatagcg ctttgtaaat ccatcaacat catttttata aaaatagtat tttcccggat
     3121 caatagatga caataaattg ttcaatgtcc tgtccgagat ggtgttgtta aattcatgat
     3181 actgtacatg ctcatttaaa aaggtactga ttatataatg gatatcgttc ggattaagaa
     3241 caactgactt tttatagcag cctgaaaaca aaacaagagc tatgcatacg tatgttagct
     3301 ttttcataaa ctatcctgtg tatgttattg taatttttta taatatggac tattatacaa
     3361 taaacattta tagaagtcaa ataattatca ctaaaggctt tgtaaacttt tttagccctt
     3421 agttacactt atgtacaata aattaatttg acattattat cattatgaca acaatgaact
     3481 gtgtaaaaac tttacgggag ctatgcaatg attggtaaat ctatgcagga attaatagca
     3541 ttgcttgaaa gtaatgcagt aacctcagtt gaaatagtac aggcattttt acaggagatg
     3601 aaccataagc aggagcttaa tgcatatatt actgtccttg atgatgatgc attacagcag
     3661 gcacagcagt cagataaacg tcgtaaggca gggaagcctc tttcacaatt tgatggcatt
     3721 cccatagcta ttaaagataa tatatgcaca cagggtatat tgacaacctg tgcatcagga
     3781 atactaaaaa attttattcc accctataat gccacagtat atcaaaagct tttgaatgca
     3841 ggatttatag tattaggtaa aacaaacatg gatgaatttg ccatgggttc aaccaccgag
     3901 acatcgtttt ttggacctac aaaaaatcct gttgatatta caaaagttcc cgggggaagc
     3961 agcggtggtt cggctgctgc ggtaaaggct tgctgcgcac cggttgcact tggttcggac
     4021 acgggtggat caatacggca gcctgcgtca ttgtgcggag tggtggggat aaagccaacc
     4081 tatggaaggg tatcgcggta tggattggtt gcctttgcat catcgcttga ccagatcggg
     4141 acatttgcaa acactgtaaa ggatgctgca gatgtattag cggttatttc gggtgttgat
     4201 cgctgtgatg caacatcaat tgctatgcct gttgattttg gttcggatac catcactggc
     4261 actattaaag gtttgcgaat aggagtcccc cgggaatatt tcaacggagt ggacaaccag
     4321 atagttcagg ctgttgaaac agtcattaaa caattggagc tcaaaggagc tatcgtacat
     4381 accatttcgt tgcaatatac cgattatgca gtacccatat actatctcat tgctactgcg
     4441 gaagcatcgt caaatttagg acggtatgat ggcgtgcgct atgggtaccg atcgcccaat
     4501 attgcaaagc tgaaggatct ctatactaaa acacgccagg aaggatttgg caaagaggtt
     4561 aaacgacgca taatactggg aacatttgcc cttagttcag gatattatga tgcctattat
     4621 ttaaaagcat taaaaggaag gcgtcttata attgatgatt ttaataaagc atttgaagag
     4681 tgtgattgca ttgttgcgcc tgtgacaaca acaacagctt ttggtattgg tgaaaagata
     4741 gctgatccat tagcgttgta tatggcagac attttaacaa tttcggctaa tctggctggc
     4801 ataccgggta tgtcggtgcc tgttgcagaa gatactcatg gtttgccaat aggtgtacag
     4861 ctcatggcgc gtcattttaa tgaaatggtt atgctcaatg ttgcgcaggc tattgaagat
     4921 agcattggtc gttagcattg cagtacattg cgcgtgtgct tatcgctata cagcattgcg
     4981 gataggaacc gtggtgacag ttcctgagca tgaatcagta gcaccagagc agttaacagc
     5041 aacagtatat tacacagcct atgttgttac tatgacaaaa ggtggtgatg aggcaaaaaa
     5101 aaataatgct gctgttgaag cactcctgaa aggaaagata gatgacgccc tcgtagtatt
     5161 agaatcaatt gttgcatata atgaaccggc agtattgaac aattatggtg tggcgctgct
     5221 tataaacggc aatagcgata gtggattgca gtatatctgc agggcagcgg tgctgcagcc
     5281 tgataacgca tactttaaaa aaaattatct ttatctccat gaatgtaaaa atgacatagt
     5341 catctttttt ttggcgatag tttgctgtat tggctgtttg catgaaccgg tggacagcga
     5401 ttattacgat gacctgtcgc tggtgaaagg aggagaaaag ccgacaccac cggtggattt
     5461 atcgttaact gttgctgacc atgaagtaac attgcaattt tataccgaca gnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatcgcatg atggcggcag ggaaagcgat
     5641 cacagtgagg tagtgtccat ggttatacca taacatggtt atagcaacgg tgcagagatt
     5701 tgcttaaatg tttttgttat ggtgtgattt actgtagcag catattttaa aaatgatagt
     5761 ggatgatatg catgaactat cgcatagcaa tggtattatt ttttattttt tatacaccgg
     5821 cactatgtgg gcaaaataag ccgtttacct ttgtccctgc ggaacaaccg ctaagcctgt
     5881 ataaagcgct ggcgtatgat attgtaccgg gaggagggca tatctatacc gggcactatg
     5941 aatatgctgc tgtttttgca accttgaaaa tttctggtgt gtatgctatg tattactatt
     6001 ataactactg gcactatcgc ggttcattat accgctcggc caaaaaggca aatgcagcta
     6061 ttgaccctga gcatgatttg cagtttaaag atcctgaagg cggttataaa actgttgaag
     6121 agttccgaca agcatatgac cgtgcagcgc attattttac gttaagtgta gcggcaaatg
     6181 ttgccatata tgtaacatca tggctgatga cactgtatca tgtgcaacag attaatgaag
     6241 aaaggcaacc agcatatata ggatttgccg ctcatgtgct gcttgatgct gatgcatatg
     6301 gtacggtgta tgcaggatat accgtaaagt ggtaatgatg cactcaatac agtgaagcat
     6361 ggaattgcaa aaatataggt attgccaaac cgtgaagtat taaaaataaa aaaaggcttt
     6421 tataaaaagc ctttttttat cggggtgtac gaggctcgaa ctcgcgacct cctgcgtgac
     6481 aggcaggcac tctaaccagg ctgagctaac acccctcaac gcttacaact atactacaaa
     6541 tggtaaatgg agtcaacttc tgtcaattaa aaaaatcacg tttttatata tattgcaaaa
     6601 aatatttaaa aaaagcttta aattttttta taactattat atctgtaaaa ttgtctgcta
     6661 ctattaagaa gggatgatac gtgcactgaa agggagaaaa gcatgtcatt aacaattttt
     6721 gatgatatat atcatgagat acgaaaacga aatacatcag gtggtttcaa agctgtacca
     6781 tattcagatg agtttattaa atatgctgcc gggtattatg ggctctcaat ggagctgata
     6841 aaaaatatta tacagatact tattaattcg cataagatat tttcaattga gataatagcg
     6901 gaagacaaag tgcgtgatat agctcatgta gaaggttatg tagtatgcga tttagtaatt
     6961 ctcagaaagt taaaatcatt ttatcaaagt gagcttatta ttcaatatga gaatgagttc
     7021 cataagcggt taatggttca tcaagttgtt aaagagatat tccccatgct ccacagcctt
     7081 aataatactg atattggtaa agtagcaaat ctggcaatta tgcttgaaga attagagcgg
     7141 tttatggaaa aacactatga agagtttacc gaggagtgga aggcaaaacg tttagcatat
     7201 gagatcagtt tatcaaatat agatacgcaa ttagaacgaa aagttgaaaa acaaaaagtt
     7261 gcaggcaggt catcatcgcc gggtggacta aaagatcgta gaaaaaaaag ggttgtcgat
     7321 gctccgcaat ataatgaatt tattgataaa agtaaaagtt atcctctgga gcggattttt
     7381 cagatttatg gaattgattt tttcctgcgt gtgcagttgc gtaattataa atttgattat
     7441 cttaaagagc tggtagggac aaagaaaatt aaacgaaagt cagatctgct attgttaaaa
     7501 aatatgctga atgcagtact acataaccgg aatggtgatc acaatctatc tcaatatgaa
     7561 gatgccatct atgaacttat gcagacagtt aatcatcacc tttttgttga aagggatttt
     7621 taa
//
LOCUS       DAOSOX010000113         7428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_9988_length_7428_cov_7.044487, whole genome shotgun sequence.
ACCESSION   DAOSOX010000113 DAOSOX010000000
VERSION     DAOSOX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9988_length_7428_cov_7.044487"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1298
                     /locus_tag="PLX22_08570"
     CDS             123..1298
                     /locus_tag="PLX22_08570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT19989.1"
                     /translation="MYDFKRYTFPEKRKINNSIYGFFSILFILVICIFTLIPGKSNNK
                     GISAASNLNSVENKLIIDGVKMEDDMSPAETEGEIVYDDSFDDDIDINNIVTIEHNDK
                     KIDKKDDKKKEKSFDIEKLKNNDKRWHLSKYTIKKNDNIWKIAKKFNVNYKLIIMANE
                     IDNPDLLKPGKTIMVPNKNGVLYQVKKGESLYVIARQYNVSPDRIIKHNELKNANKVL
                     SGQKIFIPDAVEVIKKNPGDVLRDNKSIAKNNVTGNINKSNNINLSLLWPVQGKITSG
                     FGTRRNPLGKGKQFHCGLDISCEPGTTVKAALDGNVIFSGWKDGYGNVIILKHDKGYI
                     TVYGHNSNNLVVEGDTVEKGQEIAKSGMTGSVTGAHLHFEIRKYLTPLNPLLYFKEK"
     gene            1395..2378
                     /locus_tag="PLX22_08575"
     CDS             1395..2378
                     /locus_tag="PLX22_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19990.1"
                     /translation="MKYDNPNQIIKIFYTQNDYVAWKAIVKGRILSIGVREDAAKQEL
                     TRTVQPKTKITARLYNTEGIHKGTTLFIINSNNLIVSRFTVENIFHSQSFGDMLIGYG
                     YFRLSTIGDRVVQRFDGAYPEYAKVHKARGDYYVNIGDYGNAIKEYNEAIIMDMNYPE
                     THLALGKLYYNDNVYQFAFTEFEQAYKNKDRMVDKQDYYELLYLMAKTRYIEAYETNI
                     PKKLKEHYLKEGMKYANEALSINPEAVELLYMLGVMYYKSPEPDDVAAKKTFEKIIEL
                     DPTHIDAYIALSELYLKHKNKDKASYYSQKALTIDPANERARFLYRMTKEK"
     gene            2582..3355
                     /locus_tag="PLX22_08580"
     CDS             2582..3355
                     /locus_tag="PLX22_08580"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOT19991.1"
                     /translation="MQNEIVQSLLPLIIINAITLLSCIIFIFIYPRRKKDPEIASRMH
                     ETFLGLFFREYWYWLNKPFINFFIYFKIKPNAITTLSLITAFISAYYFYSGNFGLAGW
                     ILIVSATLDIIDGRVARSTNTVTKSGAYWDSCADRYSEGVVLLGIALYYQNNFIACLA
                     TIATLIGSELVSYTKARGEAIGITTTRGVMQRAERLFTLCIVSVLHPFFQVLFKNTNV
                     NPEIVMISAMILMAVATNLTAITRLRTIFMEIKRQENNA"
     gene            3348..4631
                     /gene="mtaB"
                     /locus_tag="PLX22_08585"
     CDS             3348..4631
                     /gene="mtaB"
                     /locus_tag="PLX22_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT19992.1"
                     /translation="MHEKTFSIKTLGCKCNQYESAQIASQLLASGFTAVPFGSLADIV
                     IINTCTVTDKSNKKCRNYIRQGARFSKSGGVIVTGCMVQTHKHEIETMDEVIAAYTNE
                     QKGQLISLLGTIDNTKQKSLNHFETKTSPFALPYMRTRGYIKIQDGCDGHCSYCIVPA
                     VRGVPQSRPIDDVINHARYLIDSQCPEIVLTGITIGKYHDKGYSLAQLIEKIVQLDGK
                     FRVRVTSVEPVHVDDGLLAMLSHHKVCNHIHLPLQSGSDAVLKHMRRPYNFTYYMHCV
                     EKMKRVLPDIAIGTDIIVGYPVEGDSDFALTLQAVTECQFAYIHQFTYSPREGTCSSE
                     CKTIPHDIVAMRAEQLRNAAQKCSLQYKKQFIHATREAVIEKGNDSIVALTDNYLKVK
                     LESNHHTRSKVGQLLPVTITTNSSTGLQGKIACDN"
     gene            complement(4685..5623)
                     /locus_tag="PLX22_08590"
     CDS             complement(4685..5623)
                     /locus_tag="PLX22_08590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOT19993.1"
                     /translation="MHKINNKIQLPAHSLIAAIGNTPLIELTKLSPSNTTILAKFEGT
                     NPSGSIKDRTAWYMIKSAIESGRLTHDKIILEPTSGNTGIGLAMVGSVLGFQVTLCMP
                     SCVSVERQHTLIAFGAHLILTDGCMGTDGAIIQAQKMCMEDPTYFMPNQFSNPANVMA
                     HYETTGVEILQQTAGAIDAFVAGLGTTGTIMGVARRLKEHNSSIKIIGVEPPPGHSIQ
                     GLKNMQEAIVPEIYNPSLLDDIITIDDETAFEYSRKLALQEGLFCGMSSGAAVAGAVK
                     VAQGMKHGNVVTILPDRGDRYLSTSLFQSICAKCPP"
     gene            complement(5799..5870)
                     /locus_tag="PLX22_08595"
     tRNA            complement(5799..5870)
                     /locus_tag="PLX22_08595"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5836..5838),aa:Gly,seq:ccc)
     gene            complement(6006..6437)
                     /locus_tag="PLX22_08600"
     CDS             complement(6006..6437)
                     /locus_tag="PLX22_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT19994.1"
                     /translation="MANATLTGVFLSTQRHPDFNRLLKAETLKARYEHSKINRLDIDR
                     TVQLLMYIMNEEKAFADEELSLSQLADELDIRQQQLSRILNEKLNKNFSGFVNEFRIE
                     EAKKMLLEEPKRSILSIGISVGFNSATSFNLAFSRYEGCSH"
     gene            7160..>7428
                     /locus_tag="PLX22_08605"
     CDS             7160..>7428
                     /locus_tag="PLX22_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT19995.1"
                     /translation="MASLDAVVKQVVSKYGNDKTRLMDIISDVQNELGCVSDDAVVQI
                     ARALNISTVDVDGVITFYHFFTKTPRGKYTIYLNNSAVAIMKGRAA"
BASE COUNT         2511 a         1146 c         1403 g         2368 t
ORIGIN      
        1 taaacaaaaa cattgaactg aaatatcacc atttaaattg attaattcat gtaacagcag
       61 atgtcgaata taatagtatg gtattttaat gacaaattta tatagttctg ggtaggcttt
      121 gcatgtatga ttttaaacga tatacctttc ctgaaaaaag gaaaataaat aatagtatct
      181 atggattttt ctctattcta tttatccttg taatctgtat ttttacatta attcccggaa
      241 aatcaaataa caaaggaatt agcgcagcgt ctaatcttaa ttcagtagaa aataagctta
      301 tcatcgatgg tgttaaaatg gaagatgata tgtcacctgc tgaaacagaa ggtgagattg
      361 tttatgacga tagttttgat gatgacattg atattaataa tatagtaact atcgaacaca
      421 atgataaaaa gatagataaa aaggatgaca aaaagaaaga aaaaagtttt gatattgaaa
      481 agcttaaaaa taacgataag cgctggcatc tttctaaata tacaataaaa aaaaatgata
      541 atatatggaa gattgcaaaa aagtttaatg ttaattataa attaataatt atggctaatg
      601 aaattgataa ccctgattta ttaaaaccag ggaaaactat aatggttccc aataaaaatg
      661 gagtattgta ccaggttaaa aagggtgagt cactatatgt aattgctcgc cagtataatg
      721 tttctcctga taggattata aaacataatg aattgaaaaa tgctaataaa gtactgtctg
      781 gtcagaaaat atttattcct gatgcagtag aggtgataaa aaaaaatccc ggtgatgtat
      841 tgagagataa taaatcaatt gcaaaaaata atgttaccgg gaatattaat aaatcaaata
      901 atataaatct ttcactgttg tggcctgttc agggtaaaat tacttccgga tttggaactc
      961 gaaggaatcc attaggtaaa ggtaagcaat ttcactgtgg attagatatc agttgtgaac
     1021 ctggtactac cgtcaaagct gctttagatg gtaatgttat attttcaggg tggaaagatg
     1081 gctatggtaa tgttataatc cttaagcatg ataaaggcta tataactgtt tatggacata
     1141 atagcaataa tttagttgtg gaaggcgata ctgtagaaaa aggtcaggaa attgcaaaaa
     1201 gtggcatgac aggttcagtt acaggagcac atttgcattt tgaaatccgc aaatatttaa
     1261 ctcctttaaa tcccttgcta tattttaagg aaaaatagat aatgaaaaat tttccatcca
     1321 taataattat aaaaatattt tttttcatta cattttgttt actgattgag ataacaattt
     1381 ttccggatac tcatatgaaa tatgataatc ccaatcagat tattaaaata ttttatactc
     1441 aaaatgatta tgttgcatgg aaggcgatag ttaagggtag aattttaagt attggtgtaa
     1501 gagaagatgc tgcaaaacag gagttaacac gtactgtaca accaaaaacg aaaataactg
     1561 cacgattata taataccgaa ggaatacata agggtactac actgtttatt attaattcaa
     1621 ataatttgat tgtttcgcgt tttaccgttg aaaacatatt tcactcacaa tcatttgggg
     1681 atatgcttat tggttatgga tattttaggt tgtcaactat tggtgatagg gttgtacaac
     1741 gttttgatgg agcatatcca gaatatgcta aagtccataa agcaagaggc gattattatg
     1801 ttaatatagg tgattatggt aatgccataa aagaatataa tgaagcaatc ataatggata
     1861 tgaattatcc tgaaacgcat ttagcattag gaaaactata ttataatgat aatgtttatc
     1921 agtttgcatt tacagaattt gaacaagcat ataaaaataa agatagaatg gtagataaac
     1981 aggattatta tgaattgtta tatttaatgg ctaaaacacg atatattgaa gcatatgaaa
     2041 ccaacatacc taagaaatta aaggaacact atcttaaaga aggtatgaaa tatgcaaatg
     2101 aggcactttc aataaatcct gaagccgtgg aactattgta tatgctggga gttatgtatt
     2161 ataaaagtcc tgaaccagat gatgtggctg ctaaaaaaac atttgaaaaa attattgagc
     2221 ttgaccctac acatattgat gcatacattg ccttatcgga attgtatcta aagcataaaa
     2281 ataaagataa agcaagttac tattcacaaa aagcactgac gatagaccct gcaaatgaac
     2341 gtgcacgatt tctctacagg atgacaaagg aaaaataata aattaagcat accattatat
     2401 tgtatatgtt aaagtatata attttttgta aagttgcgtg tattgtgctc tttgaattga
     2461 tattcaaaaa aattgacttg actgtaagta gcatttttta ctatgattct tgcctgtacc
     2521 atgaaacaaa tattttataa taaaaaaaat aaataatagg taatagtata tgattactgt
     2581 aatgcaaaat gaaatagtgc aatcgctttt gccgttgatt atcataaatg caattactct
     2641 tttgagttgc attatattca tttttattta tccacgaagg aaaaaagatc ctgagatagc
     2701 aagtcgaatg catgaaacat ttttaggttt atttttcaga gaatattggt actggcttaa
     2761 taagcctttt attaatttct ttatttattt taaaattaaa cctaatgcga ttacaactct
     2821 atcgcttatt acagctttta taagtgctta ttatttttat agtggaaatt ttggtttggc
     2881 aggatggata ttaattgtta gtgctaccct tgatattatt gatggaaggg ttgcaaggag
     2941 tactaacacc gttacaaaat ctggagcata ctgggattca tgtgctgata gatacagtga
     3001 aggtgttgtg ttattaggca ttgcacttta ttatcaaaat aattttattg catgccttgc
     3061 cactattgct acattaattg gttcggaatt agtaagttat accaaagcac gaggtgaagc
     3121 tattggcata actactacgc gaggtgttat gcagcgtgca gagcgcttgt ttactttatg
     3181 catagtttca gtgttgcatc cctttttcca ggtactattt aaaaatacaa atgtaaatcc
     3241 tgaaatagtt atgattagcg ctatgatact aatggctgtt gctactaatc ttacagcaat
     3301 taccagactg cgaactattt tcatggagat taaaagacaa gagaacaatg catgaaaaga
     3361 ctttttcaat taaaacactg ggatgtaaat gcaatcaata tgaatctgca cagatagcct
     3421 cacaattgtt agcctctggt tttactgctg tgccatttgg aagcttagct gacattgtta
     3481 tcatcaatac ctgtacggta acggataaaa gcaataaaaa gtgccgcaac tatatacggc
     3541 agggtgcgcg gttttcaaaa tcaggtggtg ttatcgttac tggatgtatg gtacaaaccc
     3601 ataaacatga aattgaaaca atggatgaag ttattgctgc ttataccaat gaacaaaaag
     3661 ggcaattgat ttcattacta ggaactatcg acaacacaaa gcaaaagtct ttaaatcatt
     3721 ttgaaactaa aacatctcca tttgccttac cctatatgcg tacccgtggt tatattaaga
     3781 ttcaggatgg ctgtgacggt cattgttctt attgtattgt tccagcagtg cgtggtgtac
     3841 cacaatcccg gccaatagat gatgttatca atcatgcccg ctaccttatt gattcacaat
     3901 gtcccgagat tgttttaacg ggtataacca ttggtaaata tcatgataaa ggttactcgc
     3961 ttgcgcagct tattgaaaaa attgttcaac tggatggcaa attcagggtc agggttacgt
     4021 cggttgagcc tgttcatgtt gatgatggac ttcttgccat gctttctcac cataaggttt
     4081 gcaatcatat acatctgccg ctgcaatcgg gctctgatgc tgttttaaaa cacatgcgtc
     4141 gtccctataa ttttacttat tacatgcatt gtgttgaaaa aatgaaaagg gtactgcctg
     4201 atatagctat tggtactgat ataattgtag ggtatccagt tgagggcgat agtgactttg
     4261 cattgacctt gcaggctgtc actgagtgcc agtttgcata tattcatcag tttacctatt
     4321 cgccgcgtga aggtacttgc tccagtgaat gtaaaactat ccctcatgac attgttgcga
     4381 tgagagcaga gcaattgcga aatgctgcac aaaaatgttc attacagtat aaaaaacaat
     4441 ttatccatgc aacacgggaa gctgttattg aaaaaggtaa tgatagtata gtagccctca
     4501 cagacaatta tctcaaggta aaacttgaaa gtaatcatca taccagaagt aaggtaggac
     4561 agctattacc tgttaccatc accactaatt caagtacagg tttgcagggt aaaattgcct
     4621 gtgataacta atactatgca tcgaatcaca ggtcaggaac tacaaatcat agtaaactaa
     4681 caaatcacgg tgggcatttt gcgcatatag actggaataa cgaagtagat aggtatctat
     4741 cgccacgatc gggcagtatg gtgacaacat taccatgctt cataccttgt gcaaccttca
     4801 cagcgccagc aacagcagca ccgcttgaca taccacagaa taatccttcc tgtaaggcta
     4861 attttcgtga atattcaaag gcagtttcat catcaatggt gataatatca tctaataagc
     4921 tgggattata tatttcagga actatcgcct cctgcatatt ttttaaaccc tgtatggaat
     4981 gaccgggtgg aggctcaaca ccaataatct tgattgaaga attatgttct ttaaggcgac
     5041 gggcaactcc cataatggta ccggtagtac ccaatcctgc tacgaaagcg tcaatagcgc
     5101 ctgctgtttg ttgaagaatt tcaacgccgg tagtttcata gtgcgccatg acattggcag
     5161 gatttgaaaa ttgattgggc ataaaatagg ttgggtcttc catgcacatc ttctgagcct
     5221 ggataattgc accatcagtt cccatacaac cgtctgtcaa tatcaggtgt gcaccaaatg
     5281 caattaaggt gtgctgacgt tcaacactta cacatgaagg catgcacagc gttacctgaa
     5341 aacctaacac cgagccaacc atagccagac caatccctgt gttgccgctg gtgggttcta
     5401 aaattatttt atcatgagtt aacctgccag attcaatagc ggacttaatc atgtaccatg
     5461 cggttcgatc ttttatagat ccgctgggat tggtaccttc aaatttagct aaaattgttg
     5521 tattggaagg tgaaagtttc gtaagttcaa tgagaggtgt attgcctatt gcagcaatta
     5581 atgaatgtgc tggaagctga attttattat taattttatg catgatggct acattacagt
     5641 atcacaaata ataatggtaa tggtatataa aaatagtata taccacaatc actacaaagt
     5701 atatcacatg ggtgatagaa aatacatata attttaaaag caagcataga gattttttat
     5761 aacgtaaatg atggaacaaa taaaaattaa cacatatgag cgggtgatgg gactcgaacc
     5821 cacggcgttc agcttgggaa gctgacattc taccactgaa ttacacccgc tttgataatt
     5881 gtgaggtaac cataacaaaa gggtattaat tttgcaatta ttttttggaa agtatgacat
     5941 ctacatcatt gcagaagtaa aatttatggc tttatagttt gtttcaaata ttttttcctg
     6001 tatgttcaat gggaacatcc ttcgtaccgt gaaaaggcaa ggttaaagga tgttgcggag
     6061 ttaaatccga ccgatatgcc tatagacagt atggatcgtt ttggttcctc aagcagcatt
     6121 ttcttcgctt cttctattct gaattcgttc acaaacccag aaaaattctt attcaacttc
     6181 tcatttaata tccgggagag ttgttgttgc cttatgtcaa gttcatcggc aagctgcgac
     6241 agggatagct cctcgtctgc aaaagctttt tcctcgttca tgatgtacat aagtagctgt
     6301 accgttctgt caatgtcgag tctatttatc tttgagtgtt cataccgtgc cttgagggtt
     6361 tcagccttta ataaacgatt aaagtcagga tgacgctgag tagatagaaa cactccggta
     6421 agggtagcat ttgccatcag cactgaccac cttgtaagtt cggttgaaaa aaatcttccg
     6481 cagccataag tgctgttgaa aggataatac agtacgggaa tagcttctta caaacgcccc
     6541 attcatgtag agttatggcc cgtttgccag caattcccta tgaacaagac agggaaggtt
     6601 gatttaatgg cacttataga aaaagcaaag gaaataacgg aagagttgcg gaaacagggg
     6661 aagtgggatg caaagtaacg gtagtaattg acatacatga cggcgggagt tgtaccgccg
     6721 tttttttttt tgattaaaaa gtgcatcacg gtatttcatt ataattcttt tttatttcat
     6781 tatggtaaaa ggagtgccat tttaaatggc attctttatt ttgtactatc aattatttaa
     6841 tctgtttcac caaaaagata tggtatacta tatttgtagt gattccaata atgtatgttt
     6901 tataatttta gttgaatttt ttatagtgga taaaacattc cattgtataa ataattatta
     6961 atggtagcat atcgtgttaa tataataaca ttatttatta caaataagta aaaaagaata
     7021 ggaggtttaa ttttttaatt gaaatataat gttattttta gaggattgta catagtaatc
     7081 tttagtagtt atatttattg tatcaatcat taaattttta atatctataa tattattaat
     7141 aaatattaaa agaggtaata tggcaagctt agatgcggtt gttaaacagg ttgtttcaaa
     7201 gtatggtaac gacaaaaccc gtcttatgga cattatttct gatgttcaga atgaactggg
     7261 ttgtgtgtcg gatgatgctg ttgttcagat tgccagggca ttaaatatct caacagtgga
     7321 tgtagatggt gttatcacct tctatcactt tttcactaaa acgcccagag gtaaatatac
     7381 aatatatctg aataacagtg cagtagctat catgaagggt cgtgctgc
//
LOCUS       DAOSOX010000114         7295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10229_length_7295_cov_8.723066, whole genome shotgun sequence.
ACCESSION   DAOSOX010000114 DAOSOX010000000
VERSION     DAOSOX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10229_length_7295_cov_8.723066"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLX22_08610"
     CDS             <1..239
                     /locus_tag="PLX22_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT19996.1"
                     /translation="VIKVHTKKAVTGIEGMLGEKGYATLDFTGRGAVQIHGELWNAYC
                     DVPVKKGDEIIVKRVDGLTLYVEKVSGEQRIRSQ"
     gene            365..1129
                     /locus_tag="PLX22_08615"
     CDS             365..1129
                     /locus_tag="PLX22_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOT19997.1"
                     /translation="MFQALPTLILLLVLLVVASIKIIREYERAVVFRLGRLLTPPTGP
                     KGPGLVIIIPFVDKMLRVSLRTVAMDVPPQDVITRDNVSIKVNAVVYFRVIDPNKAIT
                     EVEDYLYATSQLAQTTLRSILGMVELDDLLSDRERINHELQKILDQQTDPWGIKVTTV
                     EIKHVDLPQEMQRAMAKQAEAERERRAKIINAEGEFQAASKMVEAAKLMEPHPIALQL
                     RYLQTLREVATERNSTTLFPIPIDLVEPFLAHKKKL"
     gene            1364..1951
                     /locus_tag="PLX22_08620"
     CDS             1364..1951
                     /locus_tag="PLX22_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT19998.1"
                     /translation="MPKQKKRFRDKDHHVVDAYYDLCEEYDGRNAKSITTKVKKLIEK
                     DPDYFDTYLFLYDILLNEGKISQADTILNTAYERAIQLIVDDKGNWPEVLEWGWLENR
                     HIIRTILTKAIDLWDKGEIDSALDLLRKLLRSNPNDNIGARNYILAIRMNMTFSEFEH
                     RFNKGGFYDNKMFDWFEKNYKKFPDEFDWWDEMTQ"
     gene            complement(1970..4555)
                     /gene="mutS"
                     /locus_tag="PLX22_08625"
     CDS             complement(1970..4555)
                     /gene="mutS"
                     /locus_tag="PLX22_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT19999.1"
                     /translation="MGKTISMGNDNDKELTPMMRQYLAIKQNFNDDILFFRLGDFYEM
                     FFDDAKIASKILDIALTSRQNDIPMCGVPYHAADSYIARLIKAGYKVAICEQMEPPSS
                     STIVKREVIRVVTPGTVVESNLITSDENNFLGSIVIVNNTVCAGFVDVSTGDFFITTA
                     DYSNEFICTEITRFYPKEMLLYAPQQQMNYLKELLKQQDITLASINEWYYDTEYLTNI
                     ITDIYRLTNIKGLNLGTIEILTVGSLLHYLKDTHKSALSHLKFPKNLSRSDFMVLDHQ
                     TINNLEILKSQDGSRLHSLFGVLNYTQTAMGKRALERALLYPLLDKKAIESRYDIISY
                     FINNIHLLKEIQDVLSSIHDMERLLSRFTLKKYYNRDFIALQQSIEAAISIYTIIGQH
                     PDALFNMLGSISPHVQELSATIKNTIVDNPPLSSEHGRVIKEGVNAELDRLYALKIDA
                     KKWLLQYQEDEKKRLDISTLKVRYNKVIGYFIEVSKGQTSKVPDDYFRKQTLINAERY
                     TTQTLQKFETDVLQSTDSILNIENDIVNSLTNRIVELKNHIQDLAYNIGIVDLFCSLA
                     QAAYHNNYTRPALNDEGIIAIINGRHPVVEKFYIKEPFVPNDIFLDNTDNVIAIITGP
                     NMSGKSTYIRMAAIIQLMAQIGSFIPAQSGNCCITDRIFTRIGASDNISRGESTFLVE
                     MNETAIILNNATDKSLIIMDEIGRGTSTYDGMAIAWAVVEYIANYIKAKTLFATHYHE
                     LTTIKKKGIVNYTVMVKESINEVQFLHKLIPGAADKSYGIHVAKLAGIPKTVTIKADK
                     ILDKLEKSSRVELLAKMPDNEDQLEIFQATHHRLIQALNEINIENLTPLDALNEIARL
                     KRLIE"
     gene            complement(4527..5645)
                     /locus_tag="PLX22_08630"
     CDS             complement(4527..5645)
                     /locus_tag="PLX22_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT20000.1"
                     /translation="MASVLRNIIIVLLCTVTVAASEQSDYEAIQKAFSSNAAYADELV
                     KKFFTHYPESKYIPDIRMLVAASKKSVNASVADYQKIIQLYRYYNKRDEAFFSLCQLY
                     YVKSDWINLLSTSNRAIAECSTSNYLPVFYQFRAIAHFNYSNYENGLSDCETIAEHTH
                     EINALGYGLFLKTEMEKNITGYSRKYIQGITEILHGYDSTAIYPSVVLRLGQYYEQHK
                     MYNHAWSAYQYIINNYPKAPEAVTAALYAEHIKRYNPVSVQFMPDESIIADSTSIDIA
                     PDVNIPDSNNHTVHYAINIGPLYNLAKAKELQKLILKIVMPVHIARKTQSFEIYAGHF
                     STFDECMNVKIQLAEELGINGFIIEMVNQDGKNYIYGQ"
     gene            complement(5626..6945)
                     /gene="miaB"
                     /locus_tag="PLX22_08635"
     CDS             complement(5626..6945)
                     /gene="miaB"
                     /locus_tag="PLX22_08635"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT20001.1"
                     /translation="MKTHKTFTIITFGCQMNKSDSELMELSLLKSGFHKSDSYHSDIF
                     IFNTCSVRKHAEDRAIAHITETRSKYKDAIIAVAGCMAQRIAHELITEFGVNLVVGPY
                     QSPDMGNIISRYLYNSAINKYISQNADDFASRLDNELAHHSDELPWHKWVTISHGCEN
                     YCAYCIVPYVRGKLISFPSQQIIDYCTVLIDNGIREITLLGQNVNQYGQDSGDIPFYK
                     LLDKVASLRGLYKLNFITSHPKDFNDNILHVMNNNPVIARSIHLPLQSGSDAILQAMN
                     RQYTMKQYFTTIDAIHTIVPHCSVSTDLIVGFPGETDKDFEMTLNAVKTMQFDEAFTY
                     KYSPREGTRAYLQPDTIPDNEKQRRLDILITTVREVSLKQLHNHINSTTDMIVERISK
                     KSDSEVMGKTICNHPIIIKGTMDDIGKKYTVTIVSLKGSTLYGERTA"
     gene            complement(6963..>7295)
                     /locus_tag="PLX22_08640"
     CDS             complement(6963..>7295)
                     /locus_tag="PLX22_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOT20002.1"
                     /translation="NIAVIIASKPLSYWIDVFSALDACVEPVQTLDEAINNPPLYDRD
                     MIVNVTTMNGKKLKQIANPIKFSSDTYIAQYAGVSLGYNNDEILHAIGYNENEIQQLY
                     DNKTITKK"
BASE COUNT         2169 a         1302 c         1470 g         2354 t
ORIGIN      
        1 ctgtaataaa ggtacataca aaaaaagcgg tgaccggaat tgaaggtatg ctgggtgaaa
       61 agggatatgc aactcttgat tttaccggta ggggtgctgt tcagattcat ggcgaattgt
      121 ggaatgcata ttgtgatgtt ccagtaaaga agggtgatga aattattgtt aaaagagttg
      181 acgggttgac attgtatgta gagaaagtat ccggggaaca aagaattaga tcccagtagt
      241 aaggttcatc atgatgtatc ataatagttt ttttatattt acctattatt tttttagtac
      301 aaacgattta taaaaaagtt ttagcgatag tacctacaca acattctaca tcaggaggta
      361 agttatgttt caagcattac ctacccttat attacttctg gttctactgg tagttgcctc
      421 aatcaagatt atcagggaat acgaaagagc cgttgtcttt agattgggga ggctgttaac
      481 tccaccaaca gggcctaaag gtccggggct tgttatcatt ataccatttg ttgataaaat
      541 gctacgggta agccttagaa cagttgcaat ggatgtccca ccgcaggatg ttattacgag
      601 ggataatgta tcaatcaagg tcaacgcagt ggtatatttc agggtgatag atcctaataa
      661 agctattacc gaggtggaag attatctgta tgccacatca caactggcgc aaacaacatt
      721 acgaagcatt ctgggtatgg ttgaactgga tgacctgtta tcagataggg aacgcattaa
      781 tcatgaattg cagaagattc ttgatcagca gactgatcca tggggtatta aggttacaac
      841 agtagaaatt aagcatgttg atctgcctca agagatgcag cgtgctatgg caaaacaggc
      901 tgaagctgag cgtgagcgaa gagctaagat tatcaacgca gaaggggagt tccaggccgc
      961 atcaaaaatg gtagaagcag ccaagcttat ggaaccacat ccaatagccc tgcaattacg
     1021 gtatctacag accttgcgtg aggtggctac agaacgtaat tccacaacgc tgttcccaat
     1081 accaattgat ttggttgaac cttttcttgc acataaaaag aaattgtagt aatggtatag
     1141 ttattgtgaa ttgctcatga ccggtgtgct gccacccata tgggatgagg atgtataggt
     1201 atgtcattct gggcaaagtg aagaatctcg ccgttttgtg aacaaatgga ttcttcagga
     1261 ctacgtccct cagaacgaca atgttaaaaa cttaccttgc tttacgtaac agagaggcag
     1321 gtggttgaag taaataaatc tgttttcatg ggaggtgatt tctatgccca aacaaaagaa
     1381 aagatttagg gacaaagacc atcatgtggt agatgcttat tatgatctgt gtgaagagta
     1441 tgatggacgc aatgctaaat ccataactac taaagtaaag aagctgatag aaaaagatcc
     1501 tgattatttt gatacctatt tatttcttta tgatattctt ttaaatgaag gaaagatatc
     1561 gcaagctgat accatactga atacggctta tgaacgcgct atacaattaa tagttgatga
     1621 taagggcaac tggcctgaag tactggaatg gggatggtta gaaaatcggc atatcattag
     1681 aactattttg actaaggcaa ttgacctatg ggataaaggt gagatagaca gcgcattaga
     1741 tttgttaagg aaactattga ggtcaaatcc taatgataac ataggcgcac gaaattatat
     1801 actggcaatc aggatgaata tgaccttttc ggaatttgaa catcgcttta ataaaggtgg
     1861 attctacgat aataaaatgt ttgattggtt tgaaaaaaac tacaaaaaat tccccgatga
     1921 atttgattgg tgggatgaaa tgactcaatg aaaacaagct tgctggcgtt tattcaataa
     1981 gccgtttaag gcgtgcaatt tcattcagtg catcaagtgg tgtaaggttt tcaatgttta
     2041 tttcatttaa agcctgtatc aatcgatgat gagttgcctg aaatatctca agctgatctt
     2101 cattatcagg catttttgct aataattcaa cccgtgatga tttctctaat ttatctaaaa
     2161 ttttgtcagc ttttatggtt actgtttttg gtatacctgc aagtttagct acatgaatgc
     2221 catacgattt atcagcagca cccggtatca gcttatgcaa aaactgcacc tcattgatgg
     2281 attctttaac catgacggta tagtttacaa ttcccttttt tttaatcgtt gtgagttcat
     2341 gatagtgagt tgcaaatagt gtttttgctt ttatataatt tgcaatatat tccacaaccg
     2401 cccacgcaat cgccatgcca tcgtacgtgc ttgttccacg tcctatctca tccataatga
     2461 tcaaactttt atcggtagcg ttgttaagaa taatagcggt ttcattcatc tctactaaaa
     2521 atgttgattc acctcgcgaa atattatccg atgcgccaat gcgggtaaaa atgcggtcag
     2581 ttatgcagca atttccggat tgtgctggaa taaacgaacc aatctgtgcc ataagctgaa
     2641 tgatggccgc catgcggata taagtggatt tacccgacat gttgggtcct gttataatag
     2701 caataacatt gtcggtgtta tccagaaaaa tgtcattggg cacaaatggt tcttttatgt
     2761 aaaacttttc tacaaccgga tgccttccat ttataatagc aataattcct tcgtcattta
     2821 atgctgggcg tgtatagtta ttgtgatatg cagcctgtgc caatgaacaa aacaaatcta
     2881 ctattccaat gttatacgct aagtcctgaa tatgattttt cagttcaaca atacggttag
     2941 ttaaagagtt tactatatca ttttcaatgt tcagtatgct atcagttgac tgcaaaacat
     3001 ctgtttcaaa cttctgcagg gtttgcgttg tatatcgttc ggcgttgata agcgtttgct
     3061 ttctgaaata atcatcgggg acttttgatg tttgcccctt gcttacttca ataaaatagc
     3121 caatgacctt attatagcgt actttcagtg ttgagatatc aagacgtttt ttttcatctt
     3181 cctggtattg caatagccac ttctttgcgt caatttttaa cgcatataat ctgtccagtt
     3241 ctgcgttaac cccttcttta ataaccctgc catgctcgga agaaagggga ggattatcaa
     3301 cgatagtatt tttaatggtg gccgatagtt cctgtacatg ggggcttatg ctgccaagca
     3361 tattaaacaa tgcatcaggg tgttgcccga taatagtata gattgatatt gctgcctcga
     3421 tggattgctg taaagcaatg aaatcgcggt tataatattt ttttaaagta aaacgcgata
     3481 gcagacgttc catgtcatga attgatgaaa gaacatcctg gatttctttt aaaaggtgta
     3541 tgttgtttat aaaatatgaa ataatatcat agcggctttc gatagctttt ttatcaagta
     3601 atggatagag cagcgcacgt tctaacgcgc gttttcccat tgctgtttgt gtgtaattaa
     3661 gcacaccaaa aagtgaatgc aatcttgaac catcctgact ttttagtatc tctaaattat
     3721 tgatagtctg atggtcaagc accataaaat cactgcggct tagattcttt ggaaatttaa
     3781 ggtgggacag agcgctttta tgcgtatcct ttaaataatg taaaagcgaa ccaacagtca
     3841 aaatctctat ggtgccaagg tttaatcctt tgatattagt tagtcgatat atgtcggtta
     3901 tgatgttggt caggtattca gtatcataat accattcatt gatagaagct aatgtaatat
     3961 cctgctgttt cagcaattct tttaaatagt tcatctgctg ctgtggtgca tacagcagca
     4021 tctctttcgg ataaaaacgg gttatctcgg tgcaaataaa ttcattgcta taatcagcgg
     4081 ttgttatgaa aaagtctccg gttgagacat ccacaaatcc agcgcacaca gtattattga
     4141 caataactat cgaacctaaa aaattatttt catccgaagt gatgaggttg ctttcaacaa
     4201 cagtacctgg cgtaactact cgtataacct cacgtttaac aatggtgctt gatgaaggcg
     4261 gttccatttg ttcgcaaatg gcaaccttat atcctgcttt tataagacgg gcaatgtagc
     4321 tatccgcagc atgatatggc accccacaca tgggaatatc gttctgccgc gaagtgagcg
     4381 caatatccag tatctttgat gcaatttttg catcatcaaa aaacatttca taaaaatcgc
     4441 ccaatctaaa aaacagtata tcatcgttaa agttttgttt aatagcaaga tactgtcgca
     4501 tcattggtgt aagttctttg tcattatcat tgcccataga tatagttttt cccatcctga
     4561 ttgaccattt caattataaa gccattaata cccagttctt cagcaagctg tatttttaca
     4621 ttcatgcatt catcaaaggt gctgaaatgt ccagcatata tttcaaatga ttgtgttttt
     4681 cgtgctatgt gtacaggcat aacaattttt aaaatcaatt tctgaagttc cttcgctttt
     4741 gcaaggttat acaatggtcc aatgttgatt gcatagtgaa cggtatgatt gttgctatcg
     4801 ggtatattta catccggtgc tatatcaatg gatgtgctat cagcaatgat tgattcatcg
     4861 ggcataaact gtacactaac aggattatat ctctttatat gttcagcata aagcgccgca
     4921 gtcacggctt caggagcttt tggataattg ttgataatat actgataggc tgaccatgcg
     4981 tggttataca ttttatgctg ttcatagtac tgccccaatc gcaacaccac tgaagggtaa
     5041 attgcagtac tatcatagcc gtgtaaaatt tctgtaatgc cctgtatata ttttctggaa
     5101 taccctgtga tgttcttttc catttctgtt tttaaaaata atccgtatcc cagagcattt
     5161 atttcatgag tatgttcagc tattgtttca cagtcggata atccattttc gtagttactg
     5221 taattaaaat gagctatcgc cctgaactga taaaaaaccg ggagatagtt gctggtggaa
     5281 cattcagcaa ttgcacggtt gcttgttgaa agcaaattta tccaatcgct cttaacatag
     5341 tagagctgac acaatgaaaa gaaagcctca tcgcgtttgt tatagtaacg atagagctga
     5401 ataatttttt ggtagtcggc tacactggca ttaacagatt tttttgatgc agccaccagc
     5461 atacgtatat caggtatata cttgctttca gggtaatggg taaaaaattt ttttacaagc
     5521 tcatcagcgt atgcggcgtt gcttgaaaac gctttttgta tggcttcata atcagattgt
     5581 tctgaagcag caacagttac tgtgcacaac aatacaatga taatgttacg cagtacgctc
     5641 gccatacagc gttgaaccct ttaatgatac gatagttact gtatattttt tcccgatgtc
     5701 atccatggtg cccttgatga taatagggtg gttgcagata gtttttccca ttacctcact
     5761 gtccgatttt ttgctgattc gctcaacaat catatcggtt gttgagttaa tatgattgtg
     5821 taactgtttt agtgaaacct cgcgcactgt tgtaatgaga atgtcaagac gcctctgctt
     5881 ttcattgtca gggatagtat cgggctgtaa atatgctctg gtgccttcac ggggagaata
     5941 tttataggta aaggcttcat caaattgcat tgttttcacg gcattcagtg tcatttcaaa
     6001 atctttatcg gtttcaccgg ggaatcccac aatcaaatca gttgaaacag aacagtgcgg
     6061 tacaatagta tgaatggcat caatagttgt aaagtactgt ttcatagtgt actgccggtt
     6121 cattgcctgc aatattgcat ccgaaccact ttgcaatgga agatgaatac ttcgtgcaat
     6181 aacagggttg ttgttcatca catgcaaaat gttgtcatta aaatctttag gatgtgaggt
     6241 gatgaaatta agcttgtata gtccacggag tgaagctact ttatcaagaa gtttgtaaaa
     6301 tggaatatcg cccgagtcct gcccatattg attgacattc tgtcctaaaa gggtaatctc
     6361 cctgatgcca ttgtcaatta ataccgtaca gtaatcaata atctgttgtg atgggaatga
     6421 aataagctta ccccgcacat agggaacaat acaatacgca caataatttt cacatccatg
     6481 tgagatagta acccatttat gccatggcag ctcatcacta tgatgtgcca gttcattatc
     6541 aaggcgtgat gcaaaatcat ctgcattttg tgaaatatac ttatttatag cactgttgta
     6601 aagatagcgg gagatgatgt tccccatatc tggtgattga taaggtccca ccaccaggtt
     6661 tacaccaaac tctgttataa gttcatgagc gatacgttgt gccatgcaac ccgctactgc
     6721 aataatggca tctttatatt tgctgcgtgt ttcggttata tgagctatcg ccctgtcttc
     6781 tgcatgcttg cgtaccgaac aggtattgaa gataaatata tcactatggt aactgtcact
     6841 tttatgaaag cctgatttta aaagcgatag ttccataagt tcagaatcac ttttgttcat
     6901 ctggcaacca aatgttatga tggtaaaggt tttgtgggtt ttcattgttg tatactaaaa
     6961 ccttactttt ttgttattgt tttgttgtca tagagttgtt gtatctcatt ttcattatag
     7021 cctatagcgt gtaaaatttc atcattattg tagcctaacg aaacgccggc atactgtgca
     7081 atatatgtgt ctgaagaaaa tttaatggga tttgctatct gtttaagttt ttttccattc
     7141 atggttgtaa cattgacaat catatcacga tcatatagcg gtggattgtt aattgcttca
     7201 tcaagagttt gtacgggttc aacacatgca tcaagcgctg aaaaaacatc aatccagtat
     7261 gaaagaggct ttgacgcaat aatgactgca atatt
//
LOCUS       DAOSOX010000115         7278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10264_length_7278_cov_10.189949, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000115 DAOSOX010000000
VERSION     DAOSOX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10264_length_7278_cov_10.189949"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLX22_08645"
     CDS             complement(<1..512)
                     /locus_tag="PLX22_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT20003.1"
                     /translation="MDTKTLQQLSQDELIKLIQQLQLKIDLLQKGVEEEPVDYKKLIE
                     AADDIIFVLDSEGNVVYRNRAMEDLFPVNSKDAVGMHFSLYLPAIELDRATMVFNQIL
                     YEGKSIHNEKMKTFDKDGNPVYLMANLAPIKDDDGKITGLFGILRNITEMHLMEKKLK
                     ESSRRLEEKI"
     gene            complement(522..914)
                     /locus_tag="PLX22_08650"
     CDS             complement(522..914)
                     /locus_tag="PLX22_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20004.1"
                     /translation="MSNVKKAYLSILIYTIISIILSSIVYLSGGIPYGKGFAIGCLLS
                     LILTLLWIAGAIKGMKSNTLVLLSITAGGFLLRLTLLAVFAAGGVYIFMMDLPAFAIA
                     FLIGTIYSLVLEVWFFTTLSGPQNPKFR"
     gene            complement(985..1743)
                     /gene="trpA"
                     /locus_tag="PLX22_08655"
     CDS             complement(985..1743)
                     /gene="trpA"
                     /locus_tag="PLX22_08655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOT20005.1"
                     /translation="MKYNGFYLVGNYPDEETFLQAATIGLHYFDFMEVGLPFSDPIAD
                     GPVLASASHKALQNGATTDNVLKNIKKIKDYIINNNCNKKIYIMTYANKVYHTTIDRT
                     IALFADSGVDGIILADVPYKESARFKQYTDSYHLDYIHFITPESTHTQIQQVCADAKG
                     FIYAISMRGTTGNTLTLDNTIIDILQLAKQYAKIPVVLGFGIQNNHDVQQALHYADGF
                     IIGTALEKKLEEGLDAYKQFIYQLHIQDKTKNIS"
     assembly_gap    1860..1959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1960..2782)
                     /gene="trpB"
                     /locus_tag="PLX22_08660"
     CDS             complement(<1960..2782)
                     /gene="trpB"
                     /locus_tag="PLX22_08660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOT20006.1"
                     /translation="MTYPDNNGYFGTFGGMFVPETLVNALTELEEAYNYFLHNDTMQN
                     QLNELLHHYAGRPTPLYFAQRLSKQTGIQLYFKREDLLHTGAHKLNNTLGQGLLTQFM
                     GKKRIIAETGAGQHGVATATVAALLGLECTIYMGQIDIERQMPNVKRMKLLGAEVIPV
                     TSGSQTLKDAINEALRDWVRNVKTTHYLLGSVSGPHPFPTIVRDFQKIIGSETKQQFQ
                     ALYGTLPDYCVACVGGGSNAAGFFYDFIGSTVKCIGVEAAGKGLFPGGHCASLTLG"
     gene            complement(2766..>3137)
                     /locus_tag="PLX22_08665"
     CDS             complement(2766..>3137)
                     /locus_tag="PLX22_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20007.1"
                     /translation="LYYELSPHYSIIFALRVSKNFSFNTTPIIHKNDMYLFDTYDLAS
                     FGGTGKPFDWNSIAGFNQMGRTIIAGGLNSSTIPMLCSVMQPFGIDISTGIEDKPGIK
                     NINKMHAIIQTIKELGYDISR"
     assembly_gap    3138..3237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3238..3755)
                     /locus_tag="PLX22_08670"
     CDS             complement(<3238..3755)
                     /locus_tag="PLX22_08670"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOT20008.1"
                     /translation="MQFSLALMKQKKLEEKQLIIALCKEHAMRKRPMYDWSFNSKIAI
                     IAEVKKASPSKGIIKIVDPAEQAVRYQQAGAKAISVLTDSHYFGGSFDDLCHVANAVT
                     LPVLCKEFICYTEQIDAAYLCGADSVLLINAMLTASELKTLYDYTIAKHLTPLIEIHA
                     LSELDTVLTLKP"
     gene            complement(3760..4764)
                     /gene="trpD"
                     /locus_tag="PLX22_08675"
     CDS             complement(3760..4764)
                     /gene="trpD"
                     /locus_tag="PLX22_08675"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOT20009.1"
                     /translation="MNTNELITQTIEGSTLSMDQSEYLFDAIFNGQIPEISLAAVVAA
                     MKVRKEQPEEITGAIKSMRKYAVSLPITEHVFDTCGTGGDHSRSFNISSAVAIILNAM
                     GYAIAKHGNRSVTSKSGSADFYEALNIPVHLSGEEATHYFNKTKFIFLFAPDYHPAMK
                     HAAPVRKQLGVRTIFNYLGPLTNPLQPKKQMIGVFHPSFLEVYSKAAIPLGYERLVLY
                     SSTNGMDEISPVHPTTVVEIQDNNSTTMVINPEDFITVQEASSIPYDKTPQENATLFI
                     DIIQSKRPQALSKLLALNTSLALYVLDHSSSIEKNYKDALEAIHSGCVASTVSKLQEV
                     "
     gene            complement(4730..5326)
                     /locus_tag="PLX22_08680"
     CDS             complement(4730..5326)
                     /locus_tag="PLX22_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOT20010.1"
                     /translation="MMFCIIDNYDSFTYNLVEYLRVLQQEVVIFKNDEPIDSIPLERY
                     TGIILSPGPSSPQNSGTTIAVIQQATTTPLLGVCLGMQAIAYVFGGTIVHAKTIMHGK
                     TDIIEHDGSKLFENIPRTFTAVRYHSLAVSSKDFPPCLSVCARSGDGEIMALSHNHRF
                     IWGVQFHPESYASEYGMQIVKNFIGGAYEYKRAYYTDN"
     gene            complement(5323..>5579)
                     /locus_tag="PLX22_08685"
     CDS             complement(5323..>5579)
                     /locus_tag="PLX22_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOT20011.1"
                     /translation="QIEDSPRGIYAGCVGYLGFNNTMDTCITIRTAVFSPQVSYMQAG
                     AGIVYDSIPFNEYNEIVNKLQALAVSLPFAKVNSPVKEIV"
     assembly_gap    5580..5679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5680..6667)
                     /locus_tag="PLX22_08690"
     CDS             complement(<5680..6667)
                     /locus_tag="PLX22_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOT20012.1"
                     /translation="MYPTLEQCKKLSKHYNRIPIYKKLNIAEPGLLLLLKSFIEKGDV
                     IFLESAKQNKKQSRFSFLGFNPQATIVFTPPHIIIGDSYSKKIIHCTEPFKFIDDYIA
                     RYRSPSNPRFGLYNGGLSGYFAYDMVNYTGHLRQRVHEDCVHPLMVLHHIDDFICYDN
                     KYNIYYIATSIYTDNNSVDGAYNNAIDHLCKLEDDIIHIIQHTSLPYLPAYSDIIPIA
                     FQTPAEKFMDDVTKAKTLIEDGEALQIVLSMRALINEAVDPYRFYLKLRQVNPSPYMF
                     YMKYNDLHIAGSSPEIHVKVCNNTAMLRPIAGTIAQKKTKTGNAMQKTLLLHN"
BASE COUNT         1971 a         1422 c         1415 g         2170 t
ORIGIN      
        1 ttaatctttt cttccagcct tcgggagctt tctttcagtt ttttttccat caggtgcatt
       61 tcggtgatat tacgcagtat gccaaataat ccggtaattt ttccgtcatc atctttgata
      121 ggagcaaggt ttgccattaa gtaaacagga tttccgtcct tatcaaacgt cttcatcttt
      181 tcattatgga tggatttacc ctcatagagt atctgattaa acaccatggt ggctctgtct
      241 aattcaatgg caggcaggta taatgaaaaa tgcatgccga cggcgtcttt actgttaaca
      301 gggaaaaggt cttccatggc tctgttgcga tagactacat ttccttcact gtccagcaca
      361 aagatgatgt catccgcagc ttcaatgagc tttttatagt caacgggttc ttcttctacg
      421 cccttttgca aaagatcaat ttttaattga agctgctgaa ttaatttgat taattcatcc
      481 tgtgaaagtt gctgtaatgt ttttgtatcc ataacacttt tttacctgaa cttaggattt
      541 tgcggaccgc tcagcgtggt aaaaaaccat acttccagaa ctaaactgta aatagtgcca
      601 ataagaaatg ctatagcaaa ggctggcaaa tccatcatga agatgtaaac accgcctgca
      661 gcaaaaaccg ctaaaagagt aagccgtagc aaaaagccgc ctgcggttat tgacagcaaa
      721 accagagtat tagacttcat gccttttatt gcaccagcaa tccatagtag tgttaagatt
      781 aaactcaaaa ggcatccaat tgcaaatccc ttgccatatg ggatcccacc tgataaatat
      841 actatagaag aaagaataat actaatgata gtataaataa gaatgcttag atatgctttt
      901 tttacattgc tcatagtaaa cctgttcctt tacaaaagca ctatatatgc aaattaataa
      961 tgtattattt ttatatctga caggtcaact aatatttttt gtcttatcct gaatgtgtaa
     1021 ttgatagata aactgtttat atgcgtccaa tccttcctct aatttttttt ccagtgcagt
     1081 tcctatgata aaaccatcag catagtgtag tgcctgctgt acgtcatggt tgttctgtat
     1141 tccaaatccc agtacaaccg gtatctttgc gtattgcttt gctaattgca gtatatcgat
     1201 tatagtattg tccaatgtca gggtattccc agtagttcca cgcatggata tcgcatagat
     1261 aaaaccctta gcatcagcac atacctgttg tatttgagta tgtgttgatt ctggtgtaat
     1321 aaaatggatg taatctaaat gatagctatc agtatattgt ttaaagcgtg cagattcttt
     1381 ataggggacg tcagctaaaa ttattccatc aacgccacta tctgcaaaca gagcaatagt
     1441 acggtcaata gtagtatggt acaccttatt tgcataggtc ataatgtata tttttttatt
     1501 acaattattg ttgatgatgt aatccttgat ctttttaata ttttttaaaa cattatcggt
     1561 agtagcacca ttctgcaatg ctttgtggga ggctgatgcc agtacgggtc cgtcggcaat
     1621 agggtcgcta aatggcagtc ctacttccat aaaatcaaag tagtgcagtc ctatggttgc
     1681 agcctgtaaa aatgtttcct catcggggta atttccaacc agataaaaac cgttgtattt
     1741 catagcttca cttcctttaa tatggtatca agatctttgt ccccgcgtcc tgaaaggttt
     1801 attatgacat ttgagccatt gaatttttga ctattacaca aaacatagcc caatgcatgn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gcccagggtc agtgatgcac
     1981 aatgcccgcc ggggaatagt cctttccctg cagcttcaac acctatacac ttaactgtcg
     2041 acccaataaa atcataaaaa aagccagcag cgttactgcc gccaccaacg caggcaacac
     2101 aataatcagg taaggttcca tagagcgcct ggaattgctg tttggtttca gagcctataa
     2161 ttttttgaaa gtccctgaca atggttggga aggggtgtgg accgctaacc gaccccagta
     2221 gatagtgggt agttttaaca ttgcgcaccc aatcgcgcag tgcttcattg atggcgtctt
     2281 tcagcgtctg gcttcctgat gttacgggga taacctcggc acctaaaagc ttcatgcgct
     2341 tgacattggg catctgacgc tctatgtcaa tctgtcccat atagatggtg cattcaagcc
     2401 ctaaaagcgc tgcaaccgtt gcagttgcaa caccatgctg accggcacct gtttcggcaa
     2461 taatgcgttt tttccccata aattgtgtaa gcaatccctg acccagtgta ttgtttaatt
     2521 tgtgagctcc ggtatggagt aaatcctcac gcttaaaata caattgtatg ccagtttgtt
     2581 ttgaaagcct ttgagcaaag tacaacggtg ttggcctgcc tgcatagtga tgaagcaact
     2641 cgtttaattg gttctgcatt gtatcattat gcaaaaaata gttgtatgct tcttcaagct
     2701 ctgtaagtgc gttgactaaa gtttcgggca caaacattcc gccaaatgtt ccaaaataac
     2761 cattattatc gggatatgtc atagcctaac tccttgattg tttgtataat tgcatgcatc
     2821 ttattaatat ttttaatacc aggtttgtct tctatgcccg tggagatgtc aatgccaaac
     2881 ggttgcatca ccgagcacag cattgggatg gtgctgctgt ttagccctcc tgcgataatg
     2941 gttcttccca tctgattaaa tccggcaatg ctattccagt caaatggttt cccggttcct
     3001 ccaaatgatg caaggtcata cgtatcaaat agatacatat catttttatg aattatcggg
     3061 gtagtattaa aagaaaaatt tttgctaaca cgcaaagcaa aaataatgct atagtgtggc
     3121 gatagctcat agtataannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt
     3241 ggtttcagtg taagcacggt atctaattcg ctgagtgcat ggatttcaat aaggggggtc
     3301 aggtgttttg caatagtgta atcatacagt gtttttaatt ctgaagcggt aagcatcgca
     3361 ttgattaaaa gaacactgtc ggcaccgcat agatatgctg catcaatctg ctcggtatag
     3421 catataaatt ctttgcaaag tacaggcagg gttaccgcat tggcaacatg gcacaggtca
     3481 tcaaagcttc cgccaaaata atggctatcc gtaaggacgc tgatagcttt tgctcctgcc
     3541 tgctgatagc gcactgcctg ttcagcaggg tcaactattt taatgatccc ctttgatggc
     3601 gaagctttct ttacctctgc aataatagca attttactat taaaactcca atcatacatg
     3661 gggcgttttc gcatggcgtg ttctttacac agtgcaataa tcaactgttt ttcttccagc
     3721 tttttttgct tcataagtgc cagtgaaaac tgcattgcat taaacctcct gtagtttgct
     3781 aaccgtagat gcaacacaac cgctgtgtat ggcttccagc gcatccttat aatttttttc
     3841 aattgaagag ctgtgatcaa gaacatacag cgctaatgag gtattgagcg ccaatagctt
     3901 gcttaacgcc tgcggtcttt ttgactgtat gatatcaatg aaaagcgttg cattttcctg
     3961 aggcgttttg tcatagggta tgctgctggc ttcctgtact gtgataaaat cctccggatt
     4021 gataaccatg gtggtgctgt tattgtcctg tatctcaaca actgtggttg ggtgcaccgg
     4081 tgagatttca tccatcccgt tggttgaaga atacaaaaca agccgctcat aacctaacgg
     4141 aatggctgct tttgaataaa cctccagaaa cgaaggatgg aatacaccaa tcatctgctt
     4201 tttgggttga agcgggttgg tcaatggtcc taaataatta aatatggttc ttactcccag
     4261 ctgtttgcgc accggggcag catgtttcat tgcaggatga taatcaggtg caaataaaaa
     4321 gatgaatttt gttttattga aataatgggt ggcttcttct cctgataaat ggacagggat
     4381 attgagcgct tcatagaaat cagccgatcc gcttttgctt gttactgatc ggttgccatg
     4441 ttttgcaatt gcatacccca tggcattgag tataattgca actgcactgg agatgttgaa
     4501 cgatcgtgag tggtcaccgc ctgtaccgca tgtatcaaaa acatgctcgg tgatgggaag
     4561 gctcactgcg tatttgcgca ttgattttat tgctcctgtt atctcctcgg gttgctcttt
     4621 gcgtaccttc atagctgcaa ccactgcggc taatgaaatt tcaggaatct gcccattaaa
     4681 aatagcatca aagagatact ctgactggtc catggacagg gtactgcctt caattgtctg
     4741 tgtaataagc tcgtttgtat tcataagctc ctcctataaa attttttact atctgcatcc
     4801 cgtattctga tgcatatgat tcgggatgaa actgtacacc ccagataaat ctatggttat
     4861 gtgaaagcgc cataatctcg ccatcgcctg aacgggcaca tactgaaagg catggcggaa
     4921 aatccttgct gctgactgca agcgaatgat accgtactgc tgtaaatgtc ctggggatgt
     4981 tttcaaaaag tttactgccg tcatgttcta tgatgtcggt tttgccgtgc atgatggttt
     5041 tagcatgaac tatcgtaccg ccaaaaacat aggcaatagc ctgcatcccc agacatacgc
     5101 ccaatagcgg tgtggtggtt gcctgttgaa tgactgcgat ggtagtgccg gaattttgcg
     5161 gcgaggatgg acccggcgaa agtatgatac cggtatagcg ttccagaggg atggaatcga
     5221 ttggttcatc atttttaaaa atgacaacct cctgctgtaa aacacgcaga tattcaacca
     5281 gattataggt aaacgaatca tagttatcga tgatacagaa catcatacta tctccttcac
     5341 cggtgaattt acttttgcaa atggcaaact taccgccagc gcttgaagtt tattgacgat
     5401 ttcattgtat tcattgaacg gtatgctgtc gtaaacaatc cccgcaccag cctgcatata
     5461 cgacacctga ggcgaaaaca cagcagtgcg aatggtaatg caggtatcca ttgtgttgtt
     5521 aaaaccaagg tagccaacac atcctgcata gatgccgcga gggctatctt caatctggtn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gttatggagc agcaatgttt
     5701 tttgcatggc atttcccgtt tttgtttttt tctgggcgat agttcctgct atggggcgaa
     5761 gcattgcagt attgttgcac accttaacat gaatctccgg cgaactgccg gcaatatgaa
     5821 ggtcattgta tttcatataa aacatatacg gcgatggatt tacctgacgc agcttcaggt
     5881 aaaagcggta tgggtctacc gcctcattga tgagagcacg catgctcagc acaatttgca
     5941 atgcctcacc atcttctatg agcgtttttg cttttgtaac atcatccata aacttttccg
     6001 ctggggtttg aaatgctatt ggaataatat cagagtatgc aggaagatag ggaagtgatg
     6061 tatgctgtat aatatgaatg atatcgtctt caagcttgca aagatgatcg atggcgttgt
     6121 tgtatgcacc atcaaccgaa ttattatccg tatagatgct ggtagcgatg tagtagatgt
     6181 tgtacttgtt atcgtagcat ataaaatcat caatatgatg cagaaccata aggggatgga
     6241 cacaatcctc atggacacgc tggcgtaaat gtcccgtgta atttaccata tcataggcaa
     6301 aatatcctga aagcccacca ttgtataagc caaaacgcgg attggatggt gagcggtacc
     6361 gggcaatata atcatcaata aatttgaaag gttcagtgca gtgtattatt tttttagagt
     6421 aactatcgcc aataataatg tgaggaggcg taaagacgat agttgcttgt ggattaaatc
     6481 ctaaaaatga aaacctgctt tgttttttat tttgttttgc actctcaagg aagataacat
     6541 ctcctttttc aataaatgat tttaaaagca aaagcaatcc tggctctgca atgttgagct
     6601 ttttatagat tggtatgcga ttgtagtgct tgcttaactt tttacactgc tccaatgttg
     6661 ggtacacggt aaactcctta cgctgtaaaa taaaaaagcc gtggattttt taaacccacg
     6721 gcttacggaa tataccggat caataaaaaa ccgtgggttt aatgcccacc cacggcttca
     6781 aaatatagta ttgcacaata gcctaacagg tggacatcat attggtccac caccaccagt
     6841 tattgtttat tgttaatgat ctactataca tattcaaatt ttcttaacat tatcttcgac
     6901 tttgcaatga ataattataa tagtgacaga aaatcacaat ttaaaaattt gtcaatttat
     6961 tttttatcat tatgttgatt tttatcagcg ctctgttcca cagggcagct actggatagt
     7021 caggtccgta tgccaatttt tggaaaaacc cagatattta atgcaaacca caatgccatc
     7081 agtatcaaaa accagattgc ttctttcata aaaaaactcc tttgcactat acgttaactg
     7141 catgatgctc ctgtgtcgac aacaaacccg gcaccgtacg gtgtttcaat aaaatcgatg
     7201 gtgattgcag catcgcttgc aaaaaagcga gcgatatcat tatcatacag taccggtata
     7261 tcatttgcta ttactttt
//
LOCUS       DAOSOX010000116         7063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10691_length_7063_cov_6.823059, whole genome shotgun sequence.
ACCESSION   DAOSOX010000116 DAOSOX010000000
VERSION     DAOSOX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10691_length_7063_cov_6.823059"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLX22_08695"
     CDS             <1..1190
                     /locus_tag="PLX22_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20013.1"
                     /translation="IYEKPFIITSSSTIKAIAYKTGYVDSDIAITEYTILPLGNIRIT
                     NNSGVSLNPVSSWNGNEYAIVWLDNSDNNNISEIFFTKVNNNGEKISNDIRITESNYQ
                     KLRPFIIWDNNNYAVVYEVNYSGFSQILFSKLELDGTKIISDNAISDGNSIAWYPHII
                     FNEDRYLITWYDSKNSNFEIYLKIIGINGNIIIDSTRLSNSTTASRRPVIAWNGNDLG
                     IVWEENINPNSTLNYLSINRLGQIIYESTITSISGVSAFTSIAYDNYSYALVWSDMRN
                     GNAEIYFTKINSQGLKILDDIRITVATGDSIFPSLVYNGQNFAISWQDNRSGNNEIYF
                     ALINSEGLKLTNDINISNTLGVSQNPYLLWNGYEYALAWDDIIDNNREIFFTKINPDG
                     SKQ"
     gene            1299..1943
                     /locus_tag="PLX22_08700"
     CDS             1299..1943
                     /locus_tag="PLX22_08700"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT20014.1"
                     /translation="MTKGMQTKQAVIERITEHIHLYGFSNTSLQDIIALSGVKKGNLY
                     FHFKNKEALILEALQSAFNNHQQYIEKHTSQYSDPIKKIFAMLDAVFAYHAQRTFLGG
                     CIFGNTALEMADKNPAFKQFIADVFHQWTQWIAAQLDTAKEQGLLASHTPAQELSHTI
                     VAALEGGIMLSKVSKNASELNAVMTTIKNVLQLYATNKTNNKRKHNANKTGHHR"
     gene            1915..3042
                     /locus_tag="PLX22_08705"
     CDS             1915..3042
                     /locus_tag="PLX22_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOT20015.1"
                     /translation="MLTKQDIIDKALELGFDDIGFTNAEAFHEHKPFLQKIPDNYTWA
                     VASGLQLEEGCNPATVLPQAQCIVVILQNYYKYDFPKSMEPFFGRCYMYDDRVTRDGL
                     TIKIKQFIAFLRDAGIKTKTPFHLPQKAAAARAGIGTFGKNCLLYANNAAKKSSWINP
                     VTIVIDKEYEPGKPTVAAGCPDWCRNACIAACPTRALKGNCTIDSQRCISYLTYYASD
                     ITPYELREPMGLSVYGCDRCQEVCPRNKAWMSQVLPINERVAKKAPYFDLIKLLHMDE
                     KYFLHYIWAHMFYTPATDMWRWKMNVARAMGNSNDDRFIPELIAEFNNTNDQRVQGMI
                     AWALGKIGGVRAGDALHRFRAGATHSVLHEIERALDYLHHY"
     gene            3167..4366
                     /locus_tag="PLX22_08710"
     CDS             3167..4366
                     /locus_tag="PLX22_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20016.1"
                     /translation="MIPRNLEMPIHKYKSQYPVIAIAGPRQSGKTTLAQKLFHDYDYC
                     SLENLDMRLYAEEDPRGFLKSYPPPLIIDEAQRAPSLFTYLQEMVDKNSKPAGYVLTG
                     SQQFLLMEKITQSLAGRIIIFTLFPFTVNELIVKRFDKDIPSIFTVKKENKKQCNTIT
                     IDSMIFSGMFPRIHDKHLDASKWLENYTVTYIERDIRNLINIENLHQFEIFIKTVASY
                     SGQLVNYASLSNRIGISQPTVKKWLSLLETSGVIFFLQPYFKNVAKRVIKTPKLYFVD
                     TGLLCFLLSIRSQKELIGHPLYGNIFETFIIAELYKRMVHTGITKPPIYFFRDRTGNE
                     IDCIVEWEGLLTVEIKSAKTYNPEFTDGIQKCNTILNAQKGIVIYGGDHIFNQGEAMA
                     VVPWWAL"
     gene            4370..4525
                     /locus_tag="PLX22_08715"
     CDS             4370..4525
                     /locus_tag="PLX22_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20017.1"
                     /translation="MLSYPIDYCKERLSAYKVPKYVEFIDELPLTAIGKPDRKVLKQR
                     EMEKLKS"
     gene            complement(4653..5381)
                     /locus_tag="PLX22_08720"
     CDS             complement(4653..5381)
                     /locus_tag="PLX22_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20018.1"
                     /translation="MYKLILSLVCISFVFTGCTKAKIDEKNILGDWEAIKGDYQEVSF
                     YKEDGKSKFSAYLNGRLFTDGTWLIKGDSLILQLSTGETVTYTRVAVKNGILELNGGQ
                     QQYQPLRTDQQKIVELIAAVASIEGISFSKPADTQFPWNFDEGTSEKKVKGKMIKATI
                     QLTTDDYTDLANASRKVVELLTSKGFTSSDYNMTEIQSAAENGPLKVLIKQQAPISYD
                     PETDTPVSIKGQNAYLEIMCGVIE"
     gene            complement(5477..6820)
                     /locus_tag="PLX22_08725"
     CDS             complement(5477..6820)
                     /locus_tag="PLX22_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOT20019.1"
                     /translation="MRLTLHLQMSLRAESFLEEAIAHKQTLALVTLATCPHTIPDNAG
                     RGRCVVRSTLLIFFIIVAMGCGGMKYKVVNTRHTVSTGFTVHSNIYFLCDYGLYQPGR
                     QIIPMYMYSPGDVYMHALYLYSYTPATNTLKQVLQIQSPVSTNIDISNAAWVEDNGIV
                     YCIYSAGWDNIQKKVQKDVLSFNINAMNVIALSNYEKQQILKKYGTRTNKNHMLSQTD
                     ILYYVDHLPMELWLLPSPLTFCTLDDADLKEVLVQGIGDSNFKEVIFAKLQQCSKKEL
                     LAVAAGIEKRYKSLESYQKMEYAPYRERWVTRILLAAFYGLSADKNLTLAQAVYHKNN
                     DIFYKLVNNENINYCDQHGTTLCMIAAYADNIEVLSYLLKANADMDAQDAFGCTALMY
                     AVFGRAAEAMGFLIKNNADTEVVSGSNCTAWVHIDTAGMRKRYVLLTEQKVQKKQ"
     gene            complement(6780..>7063)
                     /locus_tag="PLX22_08730"
     CDS             complement(6780..>7063)
                     /locus_tag="PLX22_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HOT20020.1"
                     /translation="LIKAGGGMIAVADGKVLAIVELPVAGLLTDEPVEAVQKKVETLE
                     KAWKMLGCNLVSPFMTMALLGLPVIPELRLSNRGLVDAVNFTFTDVIAG"
BASE COUNT         2242 a         1378 c         1294 g         2149 t
ORIGIN      
        1 ttatttatga aaaaccattt ataataacat cttcctcaac aataaaagca attgcttata
       61 aaactggata tgttgattct gatattgcta taacagaata taccatcctt cctttgggaa
      121 acatcaggat aacaaacaat agtggtgttt ctctcaatcc agtttcttca tggaacggta
      181 atgaatatgc tattgtttgg ctagataaca gtgataataa taatatttct gaaatatttt
      241 ttacaaaagt aaataacaat ggagagaaaa tctctaatga tattagaata acggaaagta
      301 attatcagaa attgagaccc tttattatat gggataataa taattatgca gtggtttatg
      361 aagttaatta tagtggattt tcacaaattt tattttctaa attggaactt gatggaacaa
      421 aaataattag tgataatgct atttcagatg gtaattcaat agcatggtac ccccatataa
      481 tatttaatga agacaggtat ttgataacat ggtatgacag taagaatagc aattttgaaa
      541 tttatttaaa aattattggt ataaatggaa atattataat tgattcaaca agattatcaa
      601 attccactac agcatcaaga agaccagtca ttgcatggaa tggaaatgat cttggtatag
      661 tgtgggagga aaatattaat cccaattcta cattaaatta tttatctatc aatagacttg
      721 gccaaattat ttatgaatcc actattacaa gtattagtgg ggtttcggca ttcacgtcga
      781 tagcatatga taattattca tatgctcttg tttggtctga tatgcgtaat ggaaatgctg
      841 aaatttattt tacaaaaata aattctcaag gtcttaaaat tttagatgat atacggatta
      901 ctgttgccac aggtgattct attttcccat ctttggtata taatggtcag aatttcgcaa
      961 tttcatggca ggataatcgc agcggtaata atgaaattta ctttgcactc attaattctg
     1021 aaggattaaa attaacaaat gatataaata tttcaaatac tttaggtgtt tctcaaaatc
     1081 catatttact atggaatggt tatgaatatg cattggcatg ggatgatatt attgataata
     1141 acagggaaat tttttttaca aagataaatc ctgatggttc aaaacaatag ataaatttat
     1201 atttactaaa tactaaaatc atatatctta ttataaaatt attgacagca attgatatat
     1261 aatatagtgt accaactggt cagtacagga tacaaaccat gacaaagggc atgcagacaa
     1321 aacaggcagt cattgaacgc atcaccgagc acattcatct ttatggattt tccaatacaa
     1381 gcttacagga tattattgct ctcagcggtg ttaaaaaggg taatctgtat tttcacttta
     1441 aaaataagga agcattgata cttgaggctt tgcaatctgc atttaacaat caccagcagt
     1501 acattgaaaa acacacatcg caatactcag atccaatcaa gaaaattttt gccatgctcg
     1561 atgctgtatt tgcataccat gcacaacgca catttctggg tgggtgcata tttggcaata
     1621 ctgctcttga gatggctgat aaaaatcccg catttaaaca gtttattgct gatgtcttcc
     1681 atcaatggac acagtggatt gcagcacaac ttgatactgc caaagagcag ggattattag
     1741 ccagccacac ccctgcacag gaactatcgc acaccattgt tgccgccctt gaaggtggta
     1801 tcatgctttc aaaggtgagc aaaaatgctt ctgaactcaa cgccgttatg accacaatca
     1861 aaaatgtttt acaattgtat gctacaaaca aaaccaacaa taaaaggaaa cacaatgcta
     1921 acaaaacagg acatcataga taaagctctg gagctgggat ttgatgatat tggctttacc
     1981 aatgcggaag cattccatga gcataaacca tttttacaaa aaattcccga taactatacg
     2041 tgggctgttg catcagggct gcaacttgaa gaaggatgta accccgctac ggtgttgcct
     2101 caggcacaat gcattgtggt gattttgcaa aactactata agtatgattt tccaaaaagc
     2161 atggaaccgt tttttggcag atgctatatg tatgacgaca gggtaacgcg cgacgggctt
     2221 actatcaaga taaaacaatt cattgcattt ttacgcgatg ctggcatcaa aacaaaaacg
     2281 ccgtttcatc tgccgcagaa ggctgccgca gcacgtgcag gtattggaac gtttggcaaa
     2341 aactgtttgt tatatgcaaa caatgctgct aaaaaaagct catggattaa tccggttacg
     2401 atagttattg ataaagagta tgaacccggc aagccaactg ttgcagcagg atgtcccgac
     2461 tggtgcagaa atgcctgtat agcagcctgc cccacccgag cgctgaaagg aaactgcacc
     2521 atcgattcac agcggtgcat atcgtacctt acctattacg ccagcgatat tactccttac
     2581 gagttacgag aacccatggg gctttccgta tacggttgtg atagatgtca ggaggtttgt
     2641 ccgcgtaaca aagcgtggat gtcacaggtg cttcccatca atgaaagggt tgctaaaaaa
     2701 gcaccttatt ttgatttaat aaagctatta cacatggatg aaaagtattt tttgcactat
     2761 atatgggctc acatgtttta cacacctgca accgatatgt ggcggtggaa gatgaatgtt
     2821 gcccgtgcaa tgggaaacag caatgatgac aggtttattc cggagcttat tgctgagttt
     2881 aacaatacta atgaccagcg cgttcagggg atgattgcgt gggcgcttgg gaagattggc
     2941 ggggtcagag caggggatgc gctacatcgt tttagagctg gtgccactca ttcggtttta
     3001 catgagattg aacgtgcact tgattatttg caccattatt aaaaacgatt gtgcgcattt
     3061 attattgcaa ggcagcgtgc ataccatggt tattggttgt aaagctttat gataataaag
     3121 ccattaatat cttgacaaat ataaaatgta attttacatt agccacatga ttccacgaaa
     3181 ccttgaaatg cccatacata aatacaaatc gcaatatccg gttattgcaa tagctggtcc
     3241 ccgtcaaagc ggcaaaacaa cccttgcaca aaagctattt catgattacg actattgttc
     3301 acttgaaaac cttgatatgc gattatatgc tgaagaggac ccacgtggat ttttaaagag
     3361 ctatccacca ccactaatta ttgatgaagc acaacgagcg ccttcactgt ttacctatct
     3421 ccaggaaatg gtagataaaa acagcaaacc cgccgggtat gtactaaccg gttcgcaaca
     3481 atttcttttg atggaaaaaa taacacaatc gttagccggg cgtatcatca tatttacatt
     3541 atttccattt accgttaatg aacttatagt aaagcgtttt gataaggaca tcccttcaat
     3601 atttactgta aaaaaagaaa acaaaaaaca gtgtaatact ataaccattg atagcatgat
     3661 tttttctggt atgtttcccc gcatacatga caaacatctt gatgcatcaa agtggcttga
     3721 aaattacaca gttacctata ttgaacgtga tatccgcaat cttatcaata ttgaaaattt
     3781 acatcaattt gaaatattca taaaaacagt agcatcatac tcagggcaat tagtaaatta
     3841 tgcgtcactg tcaaacagga ttggcatatc gcagccaact gtcaaaaaat ggctttcatt
     3901 gcttgaaaca agtggtgtta tatttttcct tcaaccatat ttcaaaaatg ttgcaaaacg
     3961 agtcattaaa acaccaaagc tatattttgt cgataccggc cttttgtgtt ttctgctatc
     4021 catccgcagc cagaaagaac taatagggca ccctctttat ggcaatatct ttgaaacatt
     4081 tataattgct gagctgtata aacgaatggt tcatacaggg ataactaagc cgccaatata
     4141 ttttttccgt gacaggacag gcaatgaaat tgattgcatc gttgaatggg aaggtttgct
     4201 tactgttgaa ataaaatcag caaaaaccta taatcctgaa tttaccgatg gcatacaaaa
     4261 atgtaacacg attctcaatg cacaaaaagg tattgtcata tatggaggag accacatatt
     4321 caatcagggt gaagctatgg cagtagttcc atggtgggca ttgtagagat tgttaagtta
     4381 tccaattgac tactgtaaag aaaggctttc ggcgtataaa gtaccaaaat atgttgaatt
     4441 tattgatgaa cttcctctaa cggcaatagg gaaaccagac cgtaaggtac tgaaacagcg
     4501 tgagatggaa aagttaaaaa gctaatcatg aaataatttt gtcatctatc ttacagaaga
     4561 ttttatgata aaaagagtat tccccaatga agcaggggat actctttact gtgaacgcat
     4621 gattttttca gcaactataa aaaataaagt tattattcaa taaccccgca cattatctca
     4681 aggtatgcgt tttgcccttt aattgaaaca ggtgtatcgg tttcggggtc atagcttatg
     4741 ggtgcctgct gttttataag gactttaaga gggccatttt ctgcggcaga ttgaatttcg
     4801 gtcatattat aatctgatga agtaaacccc ttagatgtca gcaactcaac aactttcctt
     4861 gatgcatttg ccagatctgt ataatcgtca gttgttagct gtatggttgc ctttatcatt
     4921 tttcctttca cctttttttc gctggtacct tcatcaaagt tccatggaaa ttgtgtatca
     4981 gccggttttg aaaagctaat tccttctata gatgcaactg cagcaatcag ctcaactatt
     5041 ttctgttgat ctgttcggag gggttgatat tgctgctggc ccccatttaa ttccagaatg
     5101 ccatttttaa cagcaacccg tgtataggtt accgtttcac cggtactcaa ttgcagtatc
     5161 agcgagtcgc cttttatcaa ccatgtcccg tcagtgaaca gtcttccatt aagataagct
     5221 gaaaatttac ttttaccatc ttccttgtaa aaacttacct cctgataatc cccctttatt
     5281 gcttcccaat cgcccaatat attcttttca tcaattttcg cctttgtaca tcctgtaaac
     5341 acaaaagaaa tacaaacaag gcttagaatt agtttataca taccagtacc tccatatagt
     5401 atttaaaaat cgtgcattca catagttagt gcatcgtgat gctgtgatat gtgacatcaa
     5461 taaccatata atcctgctat tgcttcttct gtactttctg ctctgttaac agtacgtacc
     5521 gcttgcgcat gcctgctgta tcgatgtgca cccatgcagt acaattgctt ccggaaacaa
     5581 cttccgtatc agcattattt tttattaaaa accccatggc ttcggcagca cgcccaaaaa
     5641 ctgcatacat cagagccgta cacccaaatg catcctgtgc atccatgtct gcatttgctt
     5701 taagtaaata tgacagcacc tcaatattgt cagcatacgc agcaatcata cacagggttg
     5761 tcccgtgttg atcgcagtaa ttgatatttt cgttatttac taatttatag aatatatcat
     5821 tgtttttatg atatactgcc tgcgccagtg ttaaattttt gtcagcactt aacccataga
     5881 acgcagcaag caatattcgc gtaacccatc gctccctgta tggagcatat tccatctttt
     5941 gatagctttc aaggcttttg tatcttttct caataccggc agctactgca agtaattctt
     6001 tttttgaaca ctgttgtaat ttagcaaata tgacttcctt aaagttactg tcgccaatac
     6061 cctgaacaag aacctccttt aaatcggcat catcaagtgt gcaaaatgta agcggcgaag
     6121 gcagcaacca cagctccatt ggcaaatgat ccacataata cagaatgtca gtttgggaca
     6181 gcatgtgatt tttatttgtg cgtgtaccat attttttcag gatctgctgt ttttcatagt
     6241 tggagagagc aatgacattc attgcattga tattaaaaga caatacatct ttctgcactt
     6301 ttttctgtat attgtcccag ccagcagaat aaatgcagta cacaataccg ttatcctcca
     6361 cccatgcggc attgctaata tcaatgttgg tgctgaccgg tgattgtatc tgtaacactt
     6421 gtttaagcgt attagtggca ggagtatatg aatacagata cagtgcatgc atatacacat
     6481 caccggggct gtacatgtac ataggtatta tctgcctacc gggctgatac aaaccgtaat
     6541 cgcagagaaa ataaatattg ctatgtacgg taaagcctgt tgataccgtg tgccttgtat
     6601 taacaacctt gtatttcata cccccgcaac ccatagcaac gataataaaa aaaattaata
     6661 gtgtgcttct cacaacacac ctccctctcc cggcgttgtc tggtatcgta tgcgggcagg
     6721 ttgcaagggt taccagtgcc agtgtttgtt tatgggcgat agcttcctct aaaaagcttt
     6781 cagcccgcaa tgacatctgt aaatgtaaag ttaaccgcat caacaaggcc ccggttactg
     6841 agcctgagct ccggaataac cggtaatcct aaaagagcca ttgtcataaa cggtgaaacc
     6901 agattgcatc ctaacatttt ccaggctttt tctaaagttt caaccttttt ttgaactgct
     6961 tcaaccggtt catctgtcag caaccctgca acaggaagct ccacaatagc cagcactttg
     7021 ccatcagcaa cagctatcat cccaccacca gctttgataa gct
//
LOCUS       DAOSOX010000117         7050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10718_length_7050_cov_10.150536, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000117 DAOSOX010000000
VERSION     DAOSOX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10718_length_7050_cov_10.150536"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PLX22_08735"
     CDS             complement(<1..139)
                     /locus_tag="PLX22_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20021.1"
                     /translation="MSEQKELNQKSQALESAILQIEKQFGKGSIMRLGDEHASMKVPS
                     IS"
     gene            565..1463
                     /locus_tag="PLX22_08740"
                     /pseudo
     CDS             565..1463
                     /locus_tag="PLX22_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424975.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            2402..3400
                     /locus_tag="PLX22_08745"
     CDS             2402..3400
                     /locus_tag="PLX22_08745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOT20022.1"
                     /translation="MSLEPETLINIINIQTEVVKLGLDLAGVMDLVVQRAQQLTHADG
                     AVIELAEDDYMVYRAASGTAEKQLGLRLHKETSLSGLCVQVGHALQCSDSEEDPRVDK
                     EACRKVGLRSMVVSPLKYGDIVVGAIKVLSSKPNFFDDTATHVLDLMSELIAASMYNA
                     ERYSSNELFHRATHDQLTGVANRALFYDRLRRRLIQAQRLKEKFSIIILDMDELKQIN
                     DNWGHRAGDTAIQEFSLRIKKATRQTDTVARLGGDEFGIIVHAIKNDDDIAIVTQRIE
                     NALTQPFKFEGKKIPLQASMGWALFPNDGEELEVLIDNADQRMYTMKRMHKNNPKS"
     gene            complement(3659..5296)
                     /locus_tag="PLX22_08750"
     CDS             complement(3659..5296)
                     /locus_tag="PLX22_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT20023.1"
                     /translation="MPLTLDEAKKRGWNSIDIILVTGDAYIDHPSFGVAVIGRVLEKA
                     GYTVGIIAMPNFMDPSSIEVMGRPNLFFGVTSGNIDSMLARFTAFKKIRNDDPYVPGG
                     MGGVKPSNAIITYCNLIKQRFKDVPIVIGGIEASMRRLVHYDFIQNKLRRSILVDSRA
                     DILVYGMGESQTVEIANRLSGGQSMEGISGTVTIARNKPLNAFLLPSEKEATESNEAL
                     ITMYKQFYQNFQSKVIIQESGGRYITHYPPPQISQQQLDSYYDVPYTRKPHPVYTRTI
                     PAYTMIQYSINAHRGCAGGCSFCTLTLHQGKRIVSRSRNSIKHEITAIAAKKTIHITD
                     IGGPSANMYGMECSIGGCNRESCVFPAVCKHLKINTRGWIDLMDEALTLPNVKKVSVG
                     SGIRYDIFMRSKDCLHELSHFIAHFVSGQLKVAPEHTDSTVLRAMRKVPLYPLAEFVK
                     AFNHECKRQAKEYYIVPYLMTCQPGSTDSSVYRMRSEIEAIFGYIPFQCQAFIPLPMT
                     LSSIQYYTGSDPLTGELFFVEKNTKARRKQHGILMKR"
     gene            complement(5320..6903)
                     /locus_tag="PLX22_08755"
     CDS             complement(5320..6903)
                     /locus_tag="PLX22_08755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT20024.1"
                     /translation="MSTTTKKEKVYIFDTTLRDGEQAPGFSMNIDEKLHFADQLVKLG
                     VDIIEAGFPVSSPGDFEAVKKIAERVKGVQVAGLARANRKDIDAAWDALRNAENPRIH
                     IFISSSDIHINYQFKKTREEVLAIAVDSVKHARNYMPNIEFSPMDATRSDWNYVAQMV
                     EAVIHAGATTINIPDTVGYAMPSEFHQFITFLFNNVPNIDKAIVSVHCHNDLGLATAN
                     TLAAIEAGARQVECTINGIGERAGNTAMEELVMALKVRGDKYNVYTDINTKQIVPTSK
                     LLTHITGVNVQPNKAIVGANAFAHESGIHQDGMLKNAMTYEIMKPEDVGITKSALILG
                     KHSGRHAVIARLNNMGYNLSDEELDRFFKLFKVLADKKKQIFDEDLEVLVGEAVYKVK
                     GRYRVTNVQISTGMYSPPMTLVTIKDRNDEGVESFDVAHGNGGVDAGINAVKKITGTN
                     AKIESFNLVAITGGSDALCEVTVTVVEEIDGKQLKVFGNGVNIDISVAGILSFVDALN
                     KLDYIKQRNHIGEKIEVQL"
BASE COUNT         2242 a         1431 c         1250 g         2127 t
ORIGIN      
        1 ttgatatgga cggcaccttc atcgacgcat gttcatcgcc aagccgcatg atggagccct
       61 ttccaaactg cttctctatc tgtaaaattg ccgactccag cgcctgcgac ttctgattaa
      121 gttctttctg ttcgctcatg gttatacctc atactatact attctatact atagaacgcc
      181 tggactataa aaaattaaaa atttttttaa aaatgtaaaa aaaatctttg ttcgatagtt
      241 actgatgatt gtcttattat gtcatattat aggcaatagc aaaaataagt caacagatat
      301 accagcccca tgattataat tttcccatac aaagattaac agtatccatt caccatcact
      361 atataagaaa gaatacaaat tgtactgctt tattcatttc attgtttggc atacgatatt
      421 gcataaatgt atagtaaagt gaaatgaact tcttctcatc aatgatctcc agaaccaggc
      481 tgggcgaaat aattagcata gccaaccgcg aaaattcata tgctgaaaaa gcagaaaaat
      541 ttttactgtt aatacaacaa taatatgaaa agaacaaaag gaactcaaac cagttcgttt
      601 ggaaccaatg gaagaataaa tcatgatcca aattttataa ttcacaactc tacaaagagc
      661 ttgaatacaa tagacctgtt tctaaagagg aaaacttctt ccccaaggaa ttagaaaata
      721 ccattatcca tgcttcatct gaaaatatga acgaaatccc cgataattct attcattgta
      781 tgataacctc tccaccgtat aatgtatcaa aagaatatga caatgaccta tcgttatcag
      841 aatatttgat gtttttgaca aaagtattga aagaaacgta cagggttttg gtaaatggtt
      901 gacgcgcatg tatcaatata gcaaatcttg gcagaaaacc atatatccct ctttcagact
      961 atatttcgca gataatgata gatataggat ttgctacgcg aggtgaaatt atatggaata
     1021 aagcagcaag tgcaagccca tcaactgcat ggggaatctg gcaaagtgca gcaaatccta
     1081 ttttacgcga catccatgaa tatattttaa tcttttcaaa aggtgattac aaacgagata
     1141 aaaaaggaaa agaagatacc ataacaaaag atcaatttat ggaatggact aaatccatat
     1201 ggtcaatgaa tgcagaaagt gcaaaacgta taggacatcc tgctccattt ccagaagaat
     1261 tacctttcag gctaattcag ctttactcat ttaaagggga tattattctg gatcccttta
     1321 tgggttcagg aacaacaggt gttgctgcat taaaagcgga aaggaaattc attggttatg
     1381 aaatatcatc tgaatatatt gaactatcta aaaaacgaat tgccccgctt ctggaacaaa
     1441 ccacattaca atttaaaaac tgaacatcat tagatgattc acggtgtaat aaagatgtat
     1501 atctatggct tcactatgca tgtaagcaaa aaaatcaact tgactaaatt acaccatgag
     1561 gttaaagaat atgataatca gttctttatt gattttttat cgattatttg ttttggcatt
     1621 tttatttcaa taataaaatt tcatatggtt ttttaattca agaaggcatt ataaagggtc
     1681 ctccacagtc aataactgaa ataggccatg gaagttactt aacgataaaa gacaggggga
     1741 tattgatcat ggttttactt tcgattaaac caaaattcgt taatcaaata ataaaaggta
     1801 ataaaaaatt tgaattttaa aaaacaattt tcaaagaaaa tactgataaa gcttatattt
     1861 attcaacttt tccagataag caaattattg gatatttttt tatagaagaa atcattcaag
     1921 gccatccatc taaactatgg gaaaagttta ataaatatgc aggtatcgat gaaacagact
     1981 tttttagcta tttttccggt aaaaaaatgg ctatgccatt aaaattggac atcttgaaat
     2041 ctttgaacac cattaaaccc caatgatatt tttgataatt tttatcctcc acaatctttt
     2101 tattatatag atgatgatat ttttaaaaat catgttagca aattcagaac tatgcacaac
     2161 acgcctcttg taaaaccctg aaagcaaatt tacttatact actgttttgg ccgggtagca
     2221 attgcttatt gatttttgat gcagggcaat tagtagcaag caattcttca gcatcctgct
     2281 atgctttaaa tgtggttgac aggaaaaaaa cgtttgttaa taatttactc tataggttca
     2341 ctatggcaat gaggcaaacg cctgacagat acttttatga cgtatgcact atcatacagt
     2401 tatgagctta gaacccgaaa cattaattaa tatcataaat atccagactg aagttgtgaa
     2461 acttggtctt gaccttgctg gtgtcatgga cctggttgta caacgagcac agcaattaac
     2521 ccatgctgat ggagctgtaa tagagttagc cgaagatgat tatatggtat atcgagctgc
     2581 atcaggtact gctgaaaaac aattagggct gcgtttacac aaagaaacaa gcctttccgg
     2641 actatgtgta caggttggac acgctttaca atgtagcgat tcagaagagg acccccgtgt
     2701 tgacaaagaa gcatgccgaa aagttggttt gcgttcaatg gttgtatctc ctttaaaata
     2761 tggagatata gtggttggtg ccattaaagt tttatcttct aaaccaaatt tttttgatga
     2821 taccgctact catgttcttg accttatgtc agaattaata gccgcctcaa tgtataatgc
     2881 cgaaagatac tcgtcaaatg agctttttca tcgtgctacc catgaccagc ttactggagt
     2941 agctaatcga gcactttttt atgaccgatt gcgacggcgg cttattcaag cacaacgctt
     3001 aaaagaaaaa ttttcaatta ttattctgga tatggatgaa ttgaaacaaa taaatgacaa
     3061 ttggggacat agagcaggtg atacagcaat tcaagaattt tcattgcgta taaaaaaagc
     3121 aacacgacaa acggatactg ttgccagatt aggtggtgat gaattcggca tcattgtaca
     3181 tgccatcaaa aatgatgatg acattgcaat tgtaactcag cgaattgaaa acgcacttac
     3241 gcagcctttt aaatttgaag gcaagaaaat accattgcaa gcaagtatgg gttgggcact
     3301 atttcccaat gatggtgagg aactagaagt acttattgac aacgctgatc agcgaatgta
     3361 taccatgaaa cgaatgcata aaaataaccc taaaagttaa aggattacta tacaattaat
     3421 aaaataataa aattttaatt tttatagcaa ttgtatataa tttatcatat agtacacatt
     3481 acaaatagtt gaaatttccc ggctgtaagt ttcgatagta ttaaatggaa gaatagagca
     3541 ggaattaatc ccgagatact atacaattat acaatgagag taaaccttct ggcgataatg
     3601 aaatattggc ataatccagt acaagaaagt gcacatatgc tctcccccat tcgttgcttt
     3661 accgtttcat caaaattcca tgctgcttcc ttcgtgcttt agtattcttt tctacaaaaa
     3721 acagctctcc ggttaagggg tcagagcctg tataatactg aattgacgat aaggtcatgg
     3781 gcagcgggat aaatgcctga cattgaaacg ggatatatcc aaagattgcc tcaatttcac
     3841 tgcgcatcct atacacagag ctatcggtac tgcccggttg gcaggtcata aggtagggca
     3901 caatgtagta ttcctttgcc tgccgtttgc attcatggtt aaacgccttt acaaattctg
     3961 caagggggta taacggcacc ttgcgcatag ctctgagcac cgtgctatcg gtatgttccg
     4021 gagctacttt aagctgtccg cttacaaaat gtgcaatgaa gtgcgatagc tcatgtaagc
     4081 agtcttttga ccgcataaaa atatcatagc gtatccctga accaacagat acctttttta
     4141 catttgggag ggtcagagcc tcatccatca aatctatcca gccccgggta ttaatcttca
     4201 aatgtttaca caccgcagga aacacacaac tttctctgtt gcaaccgcca atgctgcact
     4261 ccatcccata catgtttgcc gacggaccac ctatgtcggt aatatgaatt gttttctttg
     4321 cagcaatcgc cgtaatctca tgcttaatgg agttccgtga acgcgataca atcctcttgc
     4381 cctgatgcag ggttaaggta caaaacgagc atccaccggc acaaccccgg tgagcattga
     4441 tggaatactg aatcatagtg taagctggta ttgttctggt atacacagga tgaggttttc
     4501 gagtatatgg tacgtcataa tatgaatcca attgctgctg cgaaatctgt ggcggcggat
     4561 aatgggtaat atatcgccca ccgctttcct gaatgataac cttactttga aaattctggt
     4621 aaaactgttt gtacatcgtt atcagcgctt catttgattc cgtagcctcc ttttcagagg
     4681 gaagtaaaaa ggcattcaaa ggcttattcc tggcgatagt tactgttcca gaaataccct
     4741 ccatgctctg accccctgac aggcggttag caatctctac cgtctgggac tctcccatgc
     4801 catagactaa aatatcagcc cgactatcga caagaatact tcgccgtaat ttattttgaa
     4861 taaaatcata gtgcaccaat cttcgcatcg aagcttcgat gccaccaatg acgatgggaa
     4921 catctttgaa acgctgtttg atcagattgc aataggtaat aatggcattt gacggtttta
     4981 caccgcccat gccgccggga acatagggat catcattacg tattttttta aatgcagtga
     5041 accgcgcaag cattgaatca atgttgccgc tggtaacacc aaaaaaaagg tttggcctgc
     5101 ccataacctc aattgatgat gggtccataa aattgggcat ggctataata cccactgtat
     5161 atcccgcttt ttcaagtacc cgcccaataa cagcaacacc aaatgacgga tgatcaatat
     5221 atgcatcacc cgtcaccaga ataatgtcaa tactgttcca tccacgtttt tttgcttcat
     5281 ccagagttaa cggcaaaaac aatgtatact tccattatct tataattgaa cttctatctt
     5341 ctcgccaata tggttgcgct gtttgatata gtccagctta ttcaaagcat ccacaaaaga
     5401 aaggatacct gccaccgata tatcaatgtt cacgccattg ccaaaaactt taagctgttt
     5461 tccgtcaatt tcctcaacaa ctgtcaccgt aacctcgcac aatgcatcag aaccaccggt
     5521 gatagccact aaattaaatg attcgatttt ggcatttgtc ccggtaatct ttttaactgc
     5581 atttattccc gcatcaacac caccgttgcc atgcgcaaca tcaaatgatt caactccctc
     5641 atcgtttcta tccttgatag taaccagtgt catcggaggt gaatacatac cggtagatat
     5701 ctgcacgttg gttactctgt agcgcccttt gaccttgtaa actgcttcgc caaccagtac
     5761 ttcaaggtcc tcatcaaaaa tttgcttttt cttgtcagca agcactttaa aaagtttaaa
     5821 gaaacgatcc agctcttcat cgctaagatt atatcccatg ttattcagcc ttgcaatgac
     5881 agcatggcgc cccgagtgct tccctaaaat aagagcagat tttgtaatgc caacatcctc
     5941 aggtttcata atttcatacg tcatcgcatt tttcaacata ccatcctgat gtatccctga
     6001 ttcatgtgca aaagcattgg caccaacgat ggctttattt ggttgtacat tgacacccgt
     6061 aatatgagta agcaattttg aggttggcac aatttgttta gtattgatat cagtatatac
     6121 attatactta tccccacgaa ctttgagtgc cattaccagc tcttccatgg ctgtattacc
     6181 tgcccgctcg cctataccgt tgattgtaca ttccacctga cgtgcacctg cttcaatagc
     6241 agccagtgta ttggcagtag caagtccaag atcattatgg caatgaactg aaacaattgc
     6301 cttatctata ttaggcacat tgttaaaaag aaatgtaata aactggtgaa attctgatgg
     6361 catggcatac cccaccgtgt ctggaatatt gatcgttgtt gcaccggcat gtatcaccgc
     6421 ttctaccatc tgcgcaacat agttccagtc gctccttgtt gcatccattg gcgaaaattc
     6481 aatattgggc atataatttc tggcatgttt taccgaatca acagctatgg ccagaacctc
     6541 ttcacgtgtt tttttaaatt gatagttaat atggatgtct gagcttgata taaagatgtg
     6601 aatacgcgga ttttcagcat tgcgcaaagc atcccatgcg gcgtctatat cctttctatt
     6661 tgcccgtgcc aatcctgcaa cctgtacccc ttttaccctt tcggctattt tttttaccgc
     6721 ttcaaaatcg ccaggggatg atacaggaaa tcctgcctca ataatatcca cccccaattt
     6781 taccagttga tcggcaaaat ggagtttttc atctatgttc atactaaaac cgggagcctg
     6841 ctcaccatcc cgtaacgttg tatcaaaaat ataaaccttt tctttcttag ttgtcgttga
     6901 catacttttt ctccttaatc agtcaataga tataatggaa taatagatgt tttttggtat
     6961 atagtagcaa tcgctgggaa ttcatccctg tatacctcca tacataaaat aaaaccgcca
     7021 cggggtttcc gtggcggtca ttaatttttt
//
LOCUS       DAOSOX010000118         7018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10782_length_7018_cov_9.998420, whole genome shotgun sequence.
ACCESSION   DAOSOX010000118 DAOSOX010000000
VERSION     DAOSOX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10782_length_7018_cov_9.998420"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLX22_08760"
     CDS             complement(<1..1051)
                     /locus_tag="PLX22_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT20025.1"
                     /translation="MNAALNREQVDLDATIKLQRKFFLDQKTKDIGFRIESLKKLKKA
                     IIKYEDAIYDALKKDLNKSKFEAYTTEIGFVLEEISYHLKHLKKWAKPTKVRTDLVNF
                     YSRSYIVHEPFGVVLIIAPWNYPFQLLVDPLIGAISAGNCVMLKPANYSKNVTNVIVN
                     MMNETFDREYISVYTGGREAIQEILNHRFDYIFFTGSTYLGRIVMEKASRYLTPVSLE
                     LGGKSPCIVEDDANLDVAARRIVFGKLLNAGQTCIAPDYLFVNSRIKEQLIEKIKKCI
                     ILSYGDNPKLSPDYGRIINDAQFKKLKGLIETGKIIFGGQTDERERYIAPTIMDNVKP
                     DDPIMQEEIFGPIFPI"
     gene            1316..>3228
                     /locus_tag="PLX22_08765"
     CDS             1316..>3228
                     /locus_tag="PLX22_08765"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT20026.1"
                     /translation="MSDSTSKTTALHRFFGTGGLALTCIQHALRDATVIRIATAYFEP
                     TGYNLLRTTLQNKEVRLLLGRDERSEEKIEQIVNQFIEDLSSADIENKTQFLQELLHA
                     IQNHYFTINLSKQSCPTYTSLAPKFLYQHAKLYVADRSSVVVTSANLTYHGLVQSREA
                     GIVVTSSDDVEYFVKKFDEYFEEAESIAEELLQKLEELLALYSPYQIYIRSLLLLYQI
                     PEEELIGNLKLPPLTGYQRPIVSRIVRTVDEHRGAMLVASTGLGKTVMAAHISAYMRM
                     NGSINSALVLCPAGLKNMWHKILRAARISSEEFSYYILSVEDFKKSMDVAHLLSELSH
                     IDDKTLIILDESHHMRNIGSTANVRLSHQRIKDAVNKNKATILLMTATPYSTGVDDIN
                     AQLMLLPFKEDASLFDVNKKQWEVKSAAELSTLPPVVVLTSPTVVKYYSSLDENGNRY
                     VLFLNNEKRYFPQKLHFQNIEYSNQCDVILHNLLQSTLLYHRQSDEEALLFTNDAGNR
                     APLWEAFVVHQGCSSLKQMDELLSKLSYEGGFEKVRFHNQQALTEFAIEQRKVLQNYF
                     GYNDEKIKQLLAILQQFASEKVVIFCHYRETARYIKDSLNKILPQLKVETTVDVDPAK
                     VESMVKRFAPRAN"
     assembly_gap    3229..3328
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3329..3796
                     /locus_tag="PLX22_08770"
     CDS             <3329..3796
                     /locus_tag="PLX22_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20027.1"
                     /translation="NLAKSIALFGDHDLNLDEVLQFIETADKLHTSSFIDDIAMIDTK
                     LKRDILTLPMGIRSIKQSQLKQDKVLYILFKCNNRYYPVLFDKKGVVLYDSEHMDDIM
                     NIIRSSVQEEAVPSALLLDDIDAWILKAKNEWMIKHRFNAHEVMIDCTMVIVS"
     gene            complement(4229..5197)
                     /locus_tag="PLX22_08775"
     CDS             complement(4229..5197)
                     /locus_tag="PLX22_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT20028.1"
                     /translation="MNVIVSFSPLPPEFIVNILNSHGITDIEVINVHDKPKDDILMDV
                     SRADIIIGDYTFKHSIDREMIAAMKKVKLIQQPSAGYQHIDIMACKEAQISVANTAGA
                     NAISVAEYTILAALCLLKNIFFASRTTAKGEWQQMQIRPVELYGKTWGIVGMGRIGKL
                     LAQRLTVFGVTILYYDPWRLSSEAEQLMSVTYVPFYELIKTSDIISLHCPLTEETKGL
                     LGKEELSAMKSSAIIINVARGEIIDENALADALKEGRIAGAALDVFSDEPINSNNPLL
                     KVNSDRLLLTPHVAGVSIESQMRIINLTIENIIAVISGREPVNIIV"
     gene            complement(5482..>7018)
                     /locus_tag="PLX22_08780"
     CDS             complement(5482..>7018)
                     /locus_tag="PLX22_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT20029.1"
                     /translation="IQTIDVRHEQAAVMMAHAMAVYTGKPGVCFLTAGPGFTNGITGI
                     ANACLDNAPVVVLCGRHPLRDDHKGALQEMNQIDVVKPLVKWCATCYDTRRIPEYLDM
                     AFRYALCGRPGPVFLELPPDILNVAVEDFEFHPTTDIFKVTPDSENLYKAAEIINSAR
                     KPLFIGGSGLGYSNCPLELQAFIEKTGIPFMLLNGGRGVLPDNHPLSIWNIGNVGLLM
                     TLSQADVLVAAGIRFNWLFQSGAVIPPGAKVVRIDIEPTEINRNRKADAGLFGDAGTT
                     LKALLPLVEQKNHNEWLTMLTNAGYAFIDSEIKLRQLPSEPIHPIRLVARAQEVFGTD
                     ALYVIDGGDTSYFGLVGFQSKHKAGVISQSAGLLGCLGAGIPFAIAAKLVHPDKQVVV
                     LTGDGSFGFNGFEFDTAVRHNIPFVCIVNNDCAWGMIKHSQELSIGKERVTCAELGIR
                     RYEKVVDGMGGHGEFVDSDSDIIPALTRAKDSGKPACVNVLTDPTVTSPATPLFYQSL
                     KFE"
BASE COUNT         2072 a         1412 c         1298 g         2136 t
ORIGIN      
        1 tgatgggaaa tataggacca aatatctctt cttgcataat agggtcatca ggcttaacat
       61 tatccataat tgttggggca atataacgtt cccgctcatc ggtctggccg ccaaaaataa
      121 ttttaccggt ttcaatgagc ccctttagtt ttttaaactg cgcatcattg ataatgcgcc
      181 cgtaatcagg gctaagcttt ggattgtcgc catacgaaag gatgatacat ttttttattt
      241 tttcaatgag ttgttctttt atcctggaat taacaaataa gtaatcagga gcaatgcagg
      301 tttgcccggc attgagcaac ttgccaaaga caatcctccg tgcggcaacg tctagattcg
      361 cgtcgtcctc tactatgcag gggcttttgc cgcccagttc cagagaaacg ggggtgagat
      421 accgcgatgc cttttccatt acaattctgc ctagatatgt actgcccgtg aaaaaaatat
      481 aatcaaagcg atgattgaga atttcctgga tagcctcacg tcctccggta taaacgctga
      541 tatattctct gtcaaaggtt tcattcatca tgtttacgat tacattcgtt acatttttgg
      601 aataatttgc tggttttagc ataacacagt tgcctgccga gatggcgcct atcagcggat
      661 ctacgagtaa ctgaaatgga taattccatg gcgcgatgat gagaacaaca ccaaatggtt
      721 cgtgcacaat atagcttcgt gaataaaaat tgacaaggtc tgtccttact tttgtaggtt
      781 ttgcccattt cttaagatgc tttaaatggt acgatatttc ctccaataca aaaccaatct
      841 ccgttgtata agcctcaaat tttgacttgt taaggtcctt tttcagtgca tcgtagatag
      901 catcctcgta ctttatgatg gcttttttca attttttgag gctctctatt ctgaatccaa
      961 tatcctttgt tttttgatca agaaaaaatt tcctctgcaa tttaatagtg gcatcaaggt
     1021 caacctgttc cctgtttaat gctgcattca tgcgccctcc ttcttggtat gatggtatgt
     1081 aaacttttta aatcaatctt cctgcattac tttttatcga ttgttgttct ttatgtcaag
     1141 tctttaacat gcaacaatgc acagcaaacc atcgtaaatg caggctttgc aggttgcttg
     1201 cctaccattg agcgttatac atgtactatt atggggaaga ttatattttt tactgtaaaa
     1261 aaaattaata aaaatatttg ttgaaaaata cttactgcat gtacctgtca ttattatgag
     1321 cgatagcacc agtaaaacta cagcattaca tcgtttcttt ggtacaggag gattggccct
     1381 tacatgcata cagcatgcat tgcgtgatgc taccgtcata cgtatagcaa ctgcatattt
     1441 tgaaccaacc ggatataacc tgttgaggac tacattgcaa aataaagaag tacgattgct
     1501 gttaggcagg gatgaaaggt cagaggaaaa gattgagcag atagtaaatc aattcattga
     1561 ggatttatcc tcagcagata ttgaaaataa aacacaattt ttacaggaac ttttgcatgc
     1621 aatacaaaac cattatttta ctattaatct tagcaagcag tcatgtccaa cctatacatc
     1681 attggctcca aagtttcttt accagcatgc caaactgtac gttgctgatc gcagcagtgt
     1741 tgtggtaaca tcggctaatt taacctacca tggattggtg caaagtcgcg aagcgggtat
     1801 agtggtgacc agcagtgacg atgtagaata ttttgttaag aaatttgatg agtattttga
     1861 agaagctgaa tccattgcag aagaactatt gcaaaagtta gaagaattat tagctttata
     1921 tagtccctat caaatttata tacggtcatt gctgttatta tatcaaatac ctgaagagga
     1981 gcttattggt aatcttaaac tcccaccgtt aacaggatat caaaggccta tagtatcccg
     2041 tattgtcaga actgtcgatg agcaccgggg tgctatgctt gtagcctcaa ccggactggg
     2101 gaaaacggtt atggctgctc atatttccgc ctatatgcgg atgaatggaa gtattaattc
     2161 tgcgctggta ttgtgccctg cagggttaaa aaatatgtgg cacaaaatat tacgggcagc
     2221 gagaatatcc agtgaagaat tttcatacta cattttatct gttgaagatt ttaaaaaatc
     2281 catggatgtt gcacatcttc taagtgaact atcgcacata gatgataaaa ctctcataat
     2341 tttagatgaa agccatcata tgcgtaacat tggttcaacc gccaatgtac ggctatcgca
     2401 ccagcgtatc aaggatgcag taaataaaaa taaggcaacc atattactta tgacggcaac
     2461 accctatagt acgggggtgg atgatatcaa tgcacagctt atgctgttgc ccttcaaaga
     2521 agatgcatcg ctctttgatg ttaataaaaa acaatgggag gtaaaatctg ctgccgagct
     2581 ttccacactg ccgcctgttg tggtgttaac atcccccacg gtggtaaagt attatagctc
     2641 gctggatgaa aacggcaatc gctatgtact gtttttaaat aatgaaaaaa ggtatttccc
     2701 tcaaaagctt catttccaga atattgaata ttccaatcag tgtgatgtta ttttgcacaa
     2761 tcttttgcag agcaccttgc tctatcacag acaatctgat gaagaggcat tgctctttac
     2821 aaatgatgct ggcaacaggg ccccgctgtg ggaggcattt gttgtccatc aggggtgctc
     2881 atcattgaag cagatggatg agcttttatc aaagctttcg tatgaggggg gatttgaaaa
     2941 ggttagattc cataatcagc aggcgctcac tgagtttgca atagaacagc gtaaagtttt
     3001 gcaaaattat tttgggtata atgatgaaaa aataaaacaa ctgcttgcta tattacagca
     3061 gtttgccagc gaaaaggttg ttatattctg ccattaccgt gaaactgctc gctatataaa
     3121 agattcatta aacaagatac taccacaact caaagttgaa acaacagtgg atgtcgatcc
     3181 tgcaaaggtg gaatcaatgg taaaacgttt tgcaccacgg gcaaacagnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tttagccaag tccattgcac tgtttggcga
     3361 tcatgatctc aatctggatg aggtattgca atttattgaa accgctgata agctccatac
     3421 atcaagcttc attgatgata ttgccatgat cgatacaaag cttaaacgtg acatattaac
     3481 tttacccatg ggcattcgga gtataaagca atctcaatta aaacaggata aggttttgta
     3541 tattttattc aaatgcaaca ataggtacta ccctgttctt tttgataaaa aaggtgtggt
     3601 gctatacgat agtgaacata tggatgacat tatgaacatc atccgttcat ctgtccagga
     3661 agaggccgta ccttctgcac tactgcttga tgacattgat gcatggatac tgaaagcaaa
     3721 aaatgagtgg atgattaagc accgctttaa tgcccacgaa gtaatgattg actgtactat
     3781 ggtcattgta tcgtaaaatt ctactgctgt tttaaatgat cgatgcaatc tttttttgta
     3841 tttcctcaac cattactttg tactgttcaa tgctcaatat atgaggtccc atagtatcaa
     3901 tatcaggcat tttcttcata agagctatcg ccatgtcata tatatatgtt ctttttatta
     3961 ttgtttacat aaccgtatcc ataaacccct ttaaccataa aagctttagc cagagcggtg
     4021 tggggtaaaa tgctaatgtg ttggttatgc caatcgaact tatggtgaaa cttacccggg
     4081 ttatatccat ggccaaagaa aacgtagcag aagaaccaac cgtccactgt aagagccatc
     4141 cgctgaggta tggttgcatt gaattgttgt gccttcatga tttttacctt gattaaacct
     4201 gatttaccta tagttatgaa gcacttttct acactattat attaacgggt tctctgcctg
     4261 atataaccgc aataatattc tcaatcgtta gattaataat tctcatttgt gattcgatac
     4321 taacccccgc gacatgagga gtaagaagta acctgtcgct atttactttt aacaaagggt
     4381 tatttgaatt gattggttca tcgctgaaaa catcaagcgc agcacctgct attctccctt
     4441 ctttcaatgc atcggcaagt gcattctcgt caataatctc gccacgagct acattgataa
     4501 ttattgcaga tgatttcatt gcggataatt cttctttgcc cagaagtcct ttagtttctt
     4561 ctgtaagagg acaatgcaag ctaataatat cagaagtctt tattaattca tagaaaggaa
     4621 cataggtaac gctcatcaat tgctctgctt ctgatgacaa cctccatgga tcataatata
     4681 gaatagtaac accaaacaca gtaaggcgtt gggctaataa ttttccaata cgtcccatgc
     4741 caacaatacc ccatgttttc ccataaagtt caacaggtcg tatttgcatt tgctgccatt
     4801 cgccctttgc tgttgtacgt gatgcaaaga aaatattttt taacaaacac aatgcagcaa
     4861 ggattgtata ttctgctacc gaaatagcgt ttgctccagc agtatttgca acactgattt
     4921 gcgcttcttt acaagccatt atgtcaatat gctgataccc ggcacttggt tgttgtatta
     4981 gtttaacttt tttcattgct gctatcattt cgcggtcaat ggagtgctta aatgtataat
     5041 caccaatgat tatatcagca cgactgacat ccattaggat atcatcttta ggtttatcat
     5101 ggacattaat aacttcaata tctgtaatgc catggctatt gagaatgttg acaataaatt
     5161 ctgggggcaa tggtgaaaac gatactatta cattcacttt atctccctcc ttaacatttt
     5221 atttatactc tattgttttc tttatattgc gttataaaac agctaaaaca tattaacact
     5281 acactatcta tagtgtcttt gtttaactca tagcgtatat attcattaat aaaaatcttt
     5341 tgtcaaacaa caattctcta tctatgctat taagatatat catactatca ctatcgttta
     5401 tgtgcaacgt ttcattttta actgtcaaat caaacagatg ataaaactgt tatatataac
     5461 tactgcttca attacatggt gttattcaaa ttttaaagat tggtaaaata acggtgttgc
     5521 cggacttgtt actgttgggt cagttaacac attgacacat gccggtttcc cggaatcttt
     5581 tgcacgggta agtgcaggta taatatcact gtcgctatca acaaattcac cgtgaccacc
     5641 cataccatca accacctttt catatcggcg tatgcctaac tctgcgcagg taacccgctc
     5701 tttcccaata gaaagctcct ggctatgttt tatcattccc catgcgcagt cattatttac
     5761 aatacaaaca aatggtatat tgtgacgtac tgctgtatca aattcaaaac cgttgaatcc
     5821 aaatgaacca tcgcctgtta ataccacaac ctgtttatcg ggatgaacaa gttttgcggc
     5881 aatagcaaaa ggaatgcctg ctcctaaaca acctaacagc cctgcggatt gtgaaataac
     5941 gccggcttta tgttttgact gaaatcctac caatccaaaa taggatgtgt cgccaccatc
     6001 aatcacatac agtgcatctg taccaaacac ttcctgtgct ctggcaacca gccgtatcgg
     6061 atgaattggt tcagagggta actgccgcaa tttaatctca ctatcaataa aagcataccc
     6121 ggcattggta agcatggtta accactcgtt gtgatttttt tgctctacca gtggcaacaa
     6181 agctttaagg gtagtgcccg catcaccaaa aagtcctgca tcagctttcc tgttgcggtt
     6241 aatctcggtt ggttcaatat caatgcgtac aacctttgct cctggtggta ttacagctcc
     6301 cgattggaac agccagttaa aacggatgcc ggctgcaaca agtacatcag cctgtgataa
     6361 ggtcattaac aacccaacat tgccaatatt ccagatagac agagggtggt tatcaggaag
     6421 aaccccgcgc cctccattga gcaacataaa gggaatacct gttttttcta taaaagcctg
     6481 taattctaat ggacagttgc tatatcctaa cccgcttcct ccaataaaaa gaggcttcct
     6541 ggcgctattg atgatctctg ctgctttata taaattttct gaatcaggag taaccttaaa
     6601 tatatcagtg gtaggatgaa attcaaaatc ttctactgca acgtttaaaa tatcaggtgg
     6661 tagttctaaa aaaacagggc caggccttcc acacagagca taccgaaatg ccatatctaa
     6721 atattcagga atacgccttg tgtcgtaaca ggtggcacac cattttacta gaggttttac
     6781 aacatcaatc tgattcatct cctgcagagc gcctttatga tcgtcacgca gaggatgtct
     6841 accgcacaac accacaaccg gtgcgttatc caaacatgca ttggcaatcc ctgtgatacc
     6901 attggtaaaa ccaggtcctg cagtaaggaa acacacgcct ggcttacccg tataaactgc
     6961 catggcatgc gccatcataa ccgcagcctg ttcatggcgt acgtcaatgg tttgtatg
//
LOCUS       DAOSOX010000119         6996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10824_length_6996_cov_7.190462, whole genome shotgun sequence.
ACCESSION   DAOSOX010000119 DAOSOX010000000
VERSION     DAOSOX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10824_length_6996_cov_7.190462"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PLX22_08785"
     CDS             <1..1040
                     /locus_tag="PLX22_08785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT20030.1"
                     /translation="LAVAAFLSSGYLRTYLPAYIKTPDAPLLSLWAYCGVVFVGDDFY
                     VPAMRIDPDQRSDPALHRNDGELFKTIKTIKKEYPGNSLVKQLITCSTKYGCLCARNF
                     FLSRYEAPIPTTRMCNALCLGCFSFQKDTGIHVSQYRLTKEPSPEEIAQVMLHHIERV
                     DRAVVSFGQGCEGEPLMRAKDIARAITLVRKRTNRGTIHMNTNGSKPEALTMCIDAGL
                     DSVRVSLNSPTEEYYTRYYRPKNYYYNDVLQSIALACKAGIFVSLNLFFLPGFTDAES
                     EVKNLFSFLDRFPVDMIQTRNLNIDPDYYLDTISFVDEPALGIRTLLATLRERYPDIR
                     LGYYNPPKESF"
     gene            1149..1556
                     /locus_tag="PLX22_08790"
     CDS             1149..1556
                     /locus_tag="PLX22_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOT20031.1"
                     /translation="MENIIKDPIVYAREVVGRDPLATFLGITVEEVKHGYARCAVTVK
                     PEYCNAVERAHGGIIHAVADQAFAVASNSMGTMAVALSMTLQYLQAALVGERIFAEVI
                     VIHAGRKVSSWRVDVRGVDDKIIATGDAVAYHK"
     gene            complement(1657..2277)
                     /locus_tag="PLX22_08795"
     CDS             complement(1657..2277)
                     /locus_tag="PLX22_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOT20032.1"
                     /translation="MELRAQLIEFILLTLTVSLSGVMAPGPMSAVAMNHGTQQRFAGL
                     FIASGHAFVELPLIGALFFGIGFISRFSSLTAYITLAGSIVLFILGTVTIRDIRKQIT
                     IAPHSPLIDGIALTAFNPYFFIWWFTIGLSLIQKSVVFGLYGLVIFSILHVACDYVWL
                     FLLSMVSFAGASFLGKQFKTAVSIITVVMLYSFGAYFLYDALRQIL"
     gene            2505..2678
                     /locus_tag="PLX22_08800"
     CDS             2505..2678
                     /locus_tag="PLX22_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20033.1"
                     /translation="MSRIMFDAISDGIALTRRNLLKLCGASIGAYVSHCLPIRTVAKT
                     SPNILFILSDDHR"
     gene            2694..3587
                     /locus_tag="PLX22_08805"
                     /pseudo
     CDS             2694..3587
                     /locus_tag="PLX22_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            3615..3908
                     /locus_tag="PLX22_08810"
     CDS             3615..3908
                     /locus_tag="PLX22_08810"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HOT20034.1"
                     /translation="MEGKSLKPILLSDKAYGRKAWLYEYFKEFPYNVPEIRAVRTDSH
                     IYIEYGGRRKPELYNVIDDPDERDNLLNTQKGLKLKPELKKLLEDLKMGKQYD"
     gene            3901..4431
                     /locus_tag="PLX22_08815"
     CDS             3901..4431
                     /locus_tag="PLX22_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20035.1"
                     /translation="MIEHRVEHSYVVKQNFVSRFITPIVLLLVIYIVLHNLYFESWKI
                     DNRDVQYWISFIAGIVLFVFIGFNALIVYPVLYFKGATIHERIVVSLIVQIVWIIKEL
                     VRVSEFFTFGETLYYTFNSAFLLALSGSFAFMGISEMFCRYRLKKKGIELQKIITPLP
                     VMSVVTGIIALYVFLI"
     gene            4835..4993
                     /locus_tag="PLX22_08820"
     CDS             4835..4993
                     /locus_tag="PLX22_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20036.1"
                     /translation="MKNAQLYIYKEYEEPFECAILPQVDECDRLVSAIKYFGVLIPAE
                     IFDEDRGI"
     gene            5130..5780
                     /locus_tag="PLX22_08825"
     CDS             5130..5780
                     /locus_tag="PLX22_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT20037.1"
                     /translation="MIKICAFSDIHRAYENIYRLQPEIESSDIVVIAGDIGPVMWYTN
                     WHAYKRTIAWINKPVLAVHGNWDGSLIQEFLSPYSIHAGFAIVDDVGFFGVGGSIKTP
                     LTTPIEYTEEQIHDYLYKGFEHINHLPVKVLVSHTPPKNMCDRTLFKTHAGSNSVAQF
                     CCEYNINLCICGHIHEACGIGKMGHTTVVNTGSLKRGYYASILVALNGVEATIKRL"
     gene            complement(5787..6908)
                     /locus_tag="PLX22_08830"
     CDS             complement(5787..6908)
                     /locus_tag="PLX22_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOT20038.1"
                     /translation="MKNILLYSETLAQVEYSPTHPFKPMRAKQMLDLLNRYGLLENTE
                     IIEHPVLDEELLYLFHTPDYIHALKTCDHGEFDINLLTYGLGTHDNPIFKGVYNFSLQ
                     ASGGTFLGATKLVDTDATFVFNPIGGFHHAMNDHAEGFCYINDIAIALKHLIHNGLRC
                     AYIDIDVHHGDGVQSAFYDDNRVLTISIHESGQFLFPGTGFENEIGEGAGYGYNVNIP
                     LLKDTDDEIYIYAFEEIVPPLLQSFNPDIICIQVGGDSHKDDPLAHLNLTSNGYQRVL
                     TLIKQHSKKILGTGGGGYNLYKTAALWTLAWSIFTGIKPMDNFMGVIGGMMYGPEADA
                     GSLFDNPYSVGGLTKDKCFDYARDVVAYIKQSVFPIHNI"
BASE COUNT         2210 a         1194 c         1403 g         2189 t
ORIGIN      
        1 ttttggctgt ggctgctttt ttaagctcgg gctatttaag gacatatcta ccagcataca
       61 taaagactcc cgatgctcca ctattgtcat tatgggcgta ctgcggggtg gtttttgtag
      121 gagatgattt ttatgtgcca gcaatgcgta ttgatcctga ccaacgctct gaccccgccc
      181 ttcatcgtaa tgatggtgaa ctttttaaaa caataaagac tattaagaag gaatatcccg
      241 gcaatagcct ggttaagcag ttaataacct gttccactaa atacggatgt ttatgtgcac
      301 gcaatttttt cttaagccgt tatgaggcac ctatacccac cacacgaatg tgtaatgcgc
      361 tatgtctggg ttgcttttca tttcagaaag ataccggtat acatgtttcg caatatcgcc
      421 tcacgaaaga gccatcgccc gaagagattg cgcaggtaat gctgcaccat attgaacggg
      481 ttgatagggc ggttgttagt tttggacagg gttgtgaagg cgaaccgctt atgcgggcaa
      541 aggatatagc acgggcaata acgctggttc gtaaaagaac aaacagggga acaattcata
      601 tgaataccaa tggttcaaag cctgaggctc ttactatgtg tattgacgct ggacttgatt
      661 cggtcagggt aagccttaat tccccaactg aagaatatta caccaggtac tatcgcccta
      721 aaaattatta ttataacgat gtattacaga gtattgcatt ggcctgtaaa gctggtatat
      781 ttgtttcatt aaaccttttc tttttgccgg gttttaccga tgcagagagt gaggtaaaaa
      841 atcttttttc atttcttgac agatttcctg ttgatatgat acagacacgg aatctgaata
      901 tcgatccgga ttattattta gatacgatta gttttgttga tgaacctgcg cttggtattc
      961 gtacgttgct tgctaccttg cgggagcggt atcctgatat acggttgggc tattacaatc
     1021 cgcccaaaga aagtttttag tggatgtgaa tcctgtggca attaaggaaa tcttgtaatt
     1081 attttgattc cgggtcaagc ccggaatgac tttgagaaat aatgttatct tctgagggag
     1141 tgatgtaaat ggaaaatatc attaaggacc caattgtgta tgcacgggag gtcgtaggca
     1201 gggacccatt ggctacattt ttaggaataa cggtagaaga agttaagcat ggctatgcgc
     1261 ggtgtgctgt taccgttaaa cctgaatatt gtaatgcagt tgaacgcgcg catggtggca
     1321 ttattcatgc tgtggcagat caggcatttg ctgttgcaag caattcaatg ggtacaatgg
     1381 ctgtggcttt gagcatgaca ttgcagtatc ttcaggctgc actggttggc gaacgtatat
     1441 ttgcagaggt tattgtaatc catgctggta gaaaagtaag ttcatggcgt gttgatgtac
     1501 gaggtgtaga tgataaaatt attgcaacgg gagatgctgt agcctatcac aaataaattt
     1561 gctgttgctg catggtatga tgctgactta ccatagtgtt gattatatgg cttgtataat
     1621 cactgaaggc gacaccatag atgatggtga aatatcttaa agtatctggc gtaaggcatc
     1681 atacaggaag taagcgccaa aactatataa cataacaaca gttatgatac taacagccgt
     1741 tttgaattgc tttcctaaaa atgaagcgcc tgcaaatgat accattgata aaaggaataa
     1801 ccatacataa tcgcatgcta catgcagtat agaaaaaata accaatccat ataaaccaaa
     1861 taccactgat ttttgaataa gcgacaaacc aatagtaaac caccatataa agaaatatgg
     1921 attaaaggct gtaagagcaa taccatcgat aaggggagaa tgaggagcga tagtaatttg
     1981 tttacggata tcccgtatgg ttactgtgcc aaggataaat aatacaatgc tgcctgcaag
     2041 ggtaatatat gctgtaagag aggaaaaccg cgaaataaaa cctatcccaa aaaagagtgc
     2101 tcctataagc ggaagttcca cgaaagcatg cccgcttgca atgaataatc cggcaaaccg
     2161 ttgttgtgtc ccatgattca ttgccacagc actcatgggc cctggagcca taactccaga
     2221 aagtgaaacg gtcagggtaa gcagtataaa ttcaatgagc tgtgctctta attccatggt
     2281 tgtatcctgt accagattga ttcatgatat gccaatttca agcattttct ggcgttgcat
     2341 atatttttta cagtatgcta ttggtagcgt tgtaaaactt taatgctgct atacaatgaa
     2401 aagattaatt ttcaacatta tcattgacat taccattgaa gtattatagt attaagctgt
     2461 ctgccattgc tttaaatatc ctctatacaa aaaggtggat ggcaatgtcc agaataatgt
     2521 ttgatgcaat atctgatggt atagcattaa cacgtagaaa tcttttaaag ctatgtgggg
     2581 cttcaattgg agcgtatgtt tcacactgtt tgccaattcg tacagtagca aaaacaagtc
     2641 caaatatact attcattctt tctgatgacc atcgctgaga tgctatgagc tgtatgaggc
     2701 atgctataat caagacccca catcttgata ggctttcatc tgaaggagta cattttaaaa
     2761 atgcttttgt gacaacatca ctttgcagcc cttcgcgagc aagctttctt gccggacaat
     2821 atgctcacac ccatggtgtt aaaaacaacc tgactgtatg gaataatgaa aatataacat
     2881 ctttagaaat attaaaacaa gcaggttatg atactgcttt tattgggaaa tagcacatgc
     2941 ccggaaaagg gctcccaaaa cttagaggtg ttgatagatt tatttctttt accattcagg
     3001 aaggtcaggg aagatatttt gattgtccgc tatacattga tggcgtttta acagaacgta
     3061 aaggtaaata tattactgat gatttaaccg attttgcaat agaatttatt aaaactaaac
     3121 gaaacaatcc attctgttta tatctgtcat acaaagctgt tcatcagctt ttcctgtcgc
     3181 cggatgattt aaaaaattta tataatgacg ttgatgtaaa acaattattg ccagaagaat
     3241 ctgactcgtg ggtaacaaca gtacagggtg gaacatgggc gggtacgttt ggtacattgg
     3301 aacaccatta tctcaattat tgcagaactg ttgtagcact tgacaggcag attggcagaa
     3361 tactaaaagc gttagatgaa tcaagaatat aggaaaacac tgttgttatc tatgcaggag
     3421 ataatggata cttctgggga gagcatcgtt tgattgataa aagatgggct tatgaagaag
     3481 ccatacgaat acctcttatt gtccgatatc ctggatttat taaaaatccc gggagaaagg
     3541 cttatcaaat ggtattaaat attgatctgg cacctacatt acttgattta gcgggtattt
     3601 ctataccacc acatatagaa ggaaaaagtt taaagccaat attgctgtct gataaagctt
     3661 atggaagaaa agcatggctc tatgagtatt ttaaggaatt tccctataat gttccagaaa
     3721 ttcgtgctgt ccgaactgat tcacacattt acatcgaata tggtggaagg cggaagccag
     3781 aactctataa tgttatagat gatcctgatg aaagggataa tcttttaaat acacaaaaag
     3841 gattaaagct taaaccggaa cttaaaaaat tgttagaaga tttaaagatg ggaaaacaat
     3901 atgattgaac acagggttga acacagctat gtggtaaagc aaaactttgt gtcgcggttt
     3961 attacaccaa tagtattact gcttgttatt tacatagtgc ttcacaatct ttactttgaa
     4021 tcatggaaaa ttgataatag agatgtacag tactggatct cttttatagc tggtattgtg
     4081 ttatttgtat ttattggctt taatgctctt atagtttatc ctgtattata tttcaaaggt
     4141 gcaactatac atgaaagaat tgttgtgtca ttaattgtac agatagtgtg gattataaaa
     4201 gaacttgtaa gggtaagtga attttttacc tttggggaaa cattatatta tacatttaat
     4261 tcagcgtttt tattagcact atctggttct tttgctttca tggggattag tgagatgttc
     4321 tgtcgatata gattaaaaaa gaaaggaatt gaattacaaa aaataattac acctttgcca
     4381 gtcatgtctg ttgtcaccgg aattattgct ctttatgttt ttctgatatg aggtgttggc
     4441 gaacactggt tttatattta tgtaacagga tataaattaa tttttcatta atatttctat
     4501 tactatttat gcttatatat acttcctccg gagatagata tattaacctc aaaccccggc
     4561 cattcacctt catagagcaa ggggagtatt gtttccggat tgtataattt tcatccatta
     4621 tggctgatca tacgccggcc atgtacactc cctcacttta ttcttaaatt atacatataa
     4681 catttcccgg tcattgtaat aagcctttaa aaaatatcga taatcttgta aatacaaatt
     4741 taaatatatt ttaatataaa attgtttaat caacttgatt ttaatgtaat ttatgctata
     4801 ttaaaatacg attattaaat gcagggttat ctatatgaaa aatgcacaat tatacattta
     4861 taaggagtat gaagaaccgt ttgaatgtgc aatattaccc caggttgatg aatgtgacag
     4921 gcttgtttct gctataaagt attttggtgt gttgattccg gcagaaatat ttgacgagga
     4981 taggggaata tagtttaaat attgcttcaa agaggaaaac tttcttaaag atatattcta
     5041 tatataattc ttgactttta ctatatttgc cccgcttttt cattctattt atggtttgct
     5101 atcaaaattt caaatctgga cagtaaataa tgataaaaat ttgcgccttc agtgatatac
     5161 acagagccta tgaaaatatt tatcgcttac agcccgagat tgaatcaagc gatattgttg
     5221 ttattgctgg tgatattgga cctgtgatgt ggtatactaa ctggcatgca tataaaagaa
     5281 ctatcgcgtg gattaataaa cctgtgctgg ctgtacatgg caattgggac ggcagcctga
     5341 ttcaagaatt tctatcgccc tactcaatcc atgcaggttt tgcaatagtg gatgatgtag
     5401 gattttttgg ggtaggtggt tcaataaaaa cgccattaac cactccgata gaatataccg
     5461 aagagcaaat acatgattac ttgtataaag gttttgaaca cattaaccat cttcctgtaa
     5521 aggtgctggt atcacacacc cctcctaaga acatgtgtga caggacacta ttcaaaacgc
     5581 atgcgggaag caactcggtt gcacaatttt gttgtgaata taacattaac ttatgcatat
     5641 gcggccatat tcatgaagcg tgtggtatcg ggaaaatggg ccacactaca gtggtcaata
     5701 ccggtagtct gaaaagaggg tattatgcat caatactggt ggcactcaac ggtgtggagg
     5761 caacaataaa acgattatag caacaatcag atgttatgaa tgggaaaaac agattgctta
     5821 atgtaagcaa caacatcgcg tgcgtagtca aagcatttat cctttgttaa tccaccaaca
     5881 gaatagggat tgtcgaatag agaaccagca tcagcttcag gtccatacat cattccgcca
     5941 ataacaccca taaaattatc cattggtttt atgccggtaa aaattgacca tgctaaagtc
     6001 cacagcgctg ccgttttata cagattatag cctcctccgc ctgttcccaa tattttttta
     6061 gaatgttgtt ttataagcgt tagcactctt tgatatccgt tgctggtaag gttgagatgg
     6121 gctaaaggat catccttatg actatcgccg ccaacctgaa tacaaataat gtctggatta
     6181 aaactttgca ataatggtgg gacaatttct tcaaatgcat agatatatat ttcgtcatcg
     6241 gtgtccttta acagtggtat attgacatta taaccatacc ctgcgccttc accaatctca
     6301 ttttcaaacc ctgttcccgg gaaaaggaat tgtccgcttt catggattga aatagtaagt
     6361 acccggttat catcgtaaaa tgcgctttga acgccatcac catgatgaac atcaatatca
     6421 atataagcgc agcgcaaacc attgtgtata aggtgtttta atgctatggc tatatcattg
     6481 atataacaaa aaccttcggc atgatcgttc attgcgtggt gaaatccacc aattggattg
     6541 aaaacaaaag tagcatcagt atcaactaat tttgtagctc ctaaaaaagt accacctgag
     6601 gcttgtaatg aaaaattgta taccccttta aagataggat tatcatgtgt gcccaatcca
     6661 taggtcaaga ggttaatatc aaattcacca tggtcacatg ttttaagagc atgtatataa
     6721 tcaggtgtat ggaaaagata tagtagttct tcgtcaagga cagggtgttc tattatttcg
     6781 gtattttcca ataatccata tctgtttaaa agatcgagca tctgctttgc acgcattggt
     6841 ttaaaaggat gtgttgggga atattcaacc tgtgctaaag tttcagaata aagaaggata
     6901 tttttcatac atattaaacc ctaatttgtt agcttgagac agaatgcaaa ctcatatata
     6961 ataaatcaag ataatttatt ttatttttat aacgat
//
LOCUS       DAOSOX010000120         6986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10850_length_6986_cov_6.436445, whole genome shotgun sequence.
ACCESSION   DAOSOX010000120 DAOSOX010000000
VERSION     DAOSOX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10850_length_6986_cov_6.436445"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4214)
                     /locus_tag="PLX22_08835"
     CDS             complement(<1..4214)
                     /locus_tag="PLX22_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20039.1"
                     /translation="MKPFKSFFIIMCVATVLLSCSDSSKFLFQRNDPEPEYRLFDLLD
                     PYPALYDAFDTIDQHRFNVMLSDAINADVTGTKNALQQMPAINPDPLVDIVAALRIII
                     ERIINQDNYKWRSDPYDYDNYVTDFYSFLDDLDAASPGTTNELVSILRKSIGYIQYAH
                     GHEIETVMADLIDFLKDNHGQNVGTVLPLLQEGLGKTLLRANTFYDDSSNKLGNAVVG
                     MDALLSGINDIATEDADAREALYDVIRELGAVMTAKAGDKTFADILKELMINIEDYAT
                     VGGSVYSSNASYYHDGSDYYVNTELKNGVRQMLPGLALLFIRAGDNAQASPIYDPVDN
                     NSPNPDGRSALEYLTQKLYDLKLNCGIDFTNYEVEASLKRMTQYNGFGELRSSASYKV
                     SYLDHLLYTLIASYDFGFLTKTSDGASEPYNNDQDGHCSVRKHGEPTAGIITINDSLY
                     SMVSHAKEARSSSQNCTIGWLGAYNLALDARAVTQTWDGDTGGLLTSVEITNGAQGNY
                     IYRCKNSFLSSQANNYKFYLGYDFPTLLLFSGFSAGDAGIPNGGITGIVPTSNTTTVG
                     TNNDYRTYYPYVGNGLGELNTGRWTMGWIARACWEGEGPYYYADPNAKTRTASDGKVY
                     HVYFRPDGRIYAFVCKEDPANPVYFYPNDGGNDADETDNPQSLTIDGVEFKLRANRYR
                     ASWHTDYYLLKSDYINYHEDANHPPAGWTGYYSPANVVGTTGINKFKMHSLQDTKNKG
                     EYGDDLDASTNNAGCLWFWEKIAENNTVRGCNSHEEAMYRNFQWLMLEKKFVFIMPMR
                     SYVNIHATAAWCIGVTLNIDSPLFTIIEANGMAGIATAKKTGTVGTWVKLGNRGDGEG
                     CTNYGHNQPNGANYGDSLQPADGRLWVIVKEDSSYKTSLSVLTDGDYVDINTIWSTIL
                     GNGNVFPNIVGDNLAPIARMAFVQPDLVASNSSDIGNINAAAWQNRNKLLPIIVALSG
                     DLHEKSHYDKPSDQNNHWYNFSGNHKYPLKHLRDLIAMLGNPIMRYYKTPYTGAAKGW
                     FVPQIQDQHGRGVYAMFTPKPIDSAVNFNPNSSLRSVAAVLTENSQAAADGIIPALAN
                     SKMVTKLMALLQQIGQYGYGALPIYQDGTKGSSDYADWGARRKIFYGLEQIVTRMKCN
                     KSIEQTRDYYGISYPAWMFVKNSDDIDMDVALDELIGYDDVDGNPYVWGKGLAAFVDH
                     RDPAHPNYKGWNWNNYYKLVNGLGELMSNQGATNGYYCINDDLIAMIDKTLTSFKATN
                     AQLRGVRHTLGTVLYYYYDDTSQWIIPNELKDIMTGYLPQILQEYTGYNADLLSVASN
                     MLVDDGFLEYFLTNLSSPHPSEDVFNQLYEFLNLGLIKSKDSRLWDDLNELMIGLVVR
                     LNETLEGRSNKAASFEDYTKPVYSSP"
     gene            complement(4236..4400)
                     /locus_tag="PLX22_08840"
     CDS             complement(4236..4400)
                     /locus_tag="PLX22_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20040.1"
                     /translation="MYLIHKVLSKMGLKKKSFLLGKIVLGSRFFVEIYRHVSTIKFYY
                     RVTIAYNDFF"
     gene            5451..6659
                     /locus_tag="PLX22_08845"
     CDS             5451..6659
                     /locus_tag="PLX22_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT20041.1"
                     /translation="MDLFDKCLAFTRVEEVKSFGLYPYYHPITKTEGPVVYMNNKRMI
                     MVGSNNYLGLTHDPRVKQAAIDAIEKYGTGCSGSRLINGSLDIHCKLEEALAQFVGKE
                     AACIFSTGYQTNQGAIVSLINKGEYIISDEENHASIIQGTLIAKALCGDSILVKFHHN
                     DMVDLENKISALPHDAGKLIVVDGVFSMNGHIAPLPDIVRIARKYNARIMTDDAHALG
                     VIGHDGRGTASYFGLTDQTDIIMGTFSKSLASSGGFLASSERVINYIKHNAPAIIFNA
                     SMPASNAAAALKALEIIQNEPESIQKLHNNAKRLRNGLEHLGFNVIKGETPIIPVIIG
                     DDIMTFTLWKKLFDYGVYTNPIIFPATPQGQQLIRCSVMATHTDEHIDYVLGMFEKAG
                     AELKLLHAKV"
     gene            complement(6780..>6986)
                     /locus_tag="PLX22_08850"
     CDS             complement(6780..>6986)
                     /locus_tag="PLX22_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20042.1"
                     /translation="VRKRRCDSPTKYRYSSIQCYVQEGADVGVPIDHHEYFLQLGDSF
                     SERVRRFLQYEEAYRKRYSIAQWV"
BASE COUNT         2131 a         1466 c         1157 g         2232 t
ORIGIN      
        1 tatggtgatg aatatactgg ctttgtataa tcttcaaagc ttgctgcctt atttgacctt
       61 ccttctaatg tctcatttaa tcgtactaca agaccaatca tcaattcatt gagatcatcc
      121 catagccttg aatccttcga tttaattaaa cccagattca aaaattcgta caactggtta
      181 aaaacatctt ctgatggatg tggcgatgaa aggttggtca aaaaatattc aaggaatcca
      241 tcatcaacaa gcatattgct ggcaaccgat aatagatctg cattgtaccc ggtatattcc
      301 tgtaatattt gtggcagata gccggtcatt atgtccttta gttcattggg aataatccac
      361 tgtgatgtat catcataata gtagtataat accgtaccca atgtatgacg cactcctctc
      421 aactgggcat ttgttgcttt gaagcttgtc agcgttttat caatcattgc tataaggtca
      481 tcgtttatac aataatatcc gtttgttgca ccctgattgc tcatcaactc accaagacca
      541 ttgacaagct tatagtaatt attccaattc catcctttat agttgggatg ggctggatct
      601 ctgtgatcga caaatgctgc cagtcccttg ccccatacat agggattacc atccacatca
      661 tcgtatccaa taagctcatc cagtgctaca tccatgtcaa tatcatcact atttttaaca
      721 aacatccatg caggatagct gattccataa tagtctcttg tttgctcaat actcttattg
      781 catttcatgc gagttactat ctgctccaga ccataaaata tcttacgacg ggcaccccag
      841 tcagcatagt cactgcttcc ttttgtacca tcctgataga ttggtagtgc accataacca
      901 tattgaccaa tttgttgtaa caacgccatg agctttgtaa ccattttact gtttgccaaa
      961 gccgggataa tcccatccgc agcagcttga gaattttcgg tcaacacagc agcaaccgag
     1021 cgtaatgagc tgttgggatt aaagtttaca gcactatcaa taggttttgg cgtaaacatt
     1081 gcatacacgc ccctgccatg ttgatcctga atctgtggca caaaccatcc ctttgccgca
     1141 ccggtatatg gcgttttata atagcgcata attggattgc ccagcatggc tatgaggtca
     1201 cgcagatgtt ttagtggata tttatggtta ccgctaaagt tataccagtg gttattttgg
     1261 tctgatggtt tatcataatg agatttttca tgtaaatccc ctgataatgc cacaataatg
     1321 ggcagcagtt tattacggtt ctgccatgca gcagcattta tattaccaat gtctgatgaa
     1381 ttggaagcca ctaaatcagg ttgtacaaaa gccattcgtg caataggagc caggttatca
     1441 ccaacaatat taggaaaaac attaccatta ccaaggatag tactccatat tgtattaata
     1501 tcaacataat ctccatcggt tagtacactc aaactagttt tatatgatga atcctcttta
     1561 acaataaccc atagcctgcc atctgcaggt tgcagcgaat caccataatt tgcaccattt
     1621 ggctggttgt ggccataatt tgtacatccc tctccgtcac cccggtttcc aagctttacc
     1681 catgtaccaa ctgtgccagt ttttttagct gttgcaatac cagccattcc attagcttca
     1741 attattgtaa aaagcggaga atctatattc aatgtaacac ctatacacca cgcagcagta
     1801 gcatgaatgt taacatatga tcgcatgggc attataaata caaatttctt ttccagcata
     1861 agccattgaa aatttctata catggcttct tcatgactgt tacatcctct tacagtatta
     1921 ttttcagcaa tcttctccca gaaccacaaa cacccagcat tatttgttga agcatcaagg
     1981 tcatcaccat actcaccttt gttttttgta tcctgtaatg agtgcatttt aaatttattg
     2041 ataccagtag tacccactac atttgcaggt gaataataac ccgtccatcc tgcaggtgga
     2101 tgattagcat cttcatggta gtttatataa tccgatttca gcaaataata atcagtatgc
     2161 cagcttgcac ggtatctgtt agctctgagt ttaaattcaa caccatcaat tgttaatgat
     2221 tgagggttat cagtttcatc tgcatcattg ccaccatcat ttggataaaa atatactgga
     2281 ttagcaggat cttctttaca tacaaacgca taaatcctcc catcaggtct aaaatataca
     2341 tgatatacct taccatctga tgctgttctg gtttttgcgt tgggatcagc ataatagtag
     2401 ggaccttcac cttcccagca tgcacgggca atccagccca tagtccagcg tccggtgtta
     2461 agctcaccca atccattgcc aacatacggg taataagtgc ggtaatcatt atttgtgcct
     2521 actgttgtag tatttgatgt tggaactata ccggtaattc ccccgtttgg aataccagca
     2581 tcaccagcag aaaaaccact gaataataga agagttggaa aatcataccc taaataaaat
     2641 ttatagttat tagcctgtga acttaaaaat gagtttttac aacgatatat ataattccct
     2701 tgcgcaccat ttgttatttc aactgatgtt aatagacctc ctgtatcgcc atcccatgtt
     2761 tgcgtaacag cccttgcatc cagagccaaa ttataagcac ctaaccatcc aatggtacaa
     2821 ttttggctgg agcttcttgc ttcttttgca tggcttacca tggaatataa actatcattt
     2881 atagtaataa taccagctgt tggttcacca tgtttgcgaa cagagcaatg accatcctga
     2941 tcattattgt aaggttctga agcaccatca gaagttttgg ttaaaaaacc aaagtcatac
     3001 gacgctatca aagtatacaa cagatgatct aaatacgata ctttatagct tgcactgctt
     3061 cggagctctc caaatccatt atactgggtc atccttttca gggatgcctc aacctcataa
     3121 tttgtaaaat ctattccgca gtttaacttc aaatcataca atttctgtgt taaatattcc
     3181 aatgctgagc gcccgtcggg attgggcgaa ttgttatcca ccgggtcata gataggagat
     3241 gcttgtgcat tatcgcctgc ccgtataaaa agcaacgcca aaccaggcaa catctgtcgt
     3301 acaccatttt taagttcagt attaacataa taatcgcttc catcatgata ataacttgca
     3361 ttggagctat aaacactgcc cccaactgta gcataatcct caatatttat cataagctct
     3421 ttgagtatgt cggcaaatgt tttatctccc gcttttgccg tcattaccgc acccagctcg
     3481 cgtattacat catagagcgc ttcacgtgca tctgcatcct ccgtagctat atcgtttatc
     3541 ccagacagca gcgcatccat accaacaacc gcattcccca atttattgct gctatcgtca
     3601 taaaatgtgt ttgcccgtaa taatgtttta cccaatcctt cctgcaacag tggcaacacc
     3661 gtgccaacat tctgcccatg gttatccttt aaaaaatcaa tcaaatctgc cataactgtc
     3721 tctatttcat gaccatgagc atactgaata tagcctatgg actttctcag gatacttacc
     3781 agctcgttgg tagtaccagg gcttgctgca tctaaatcat ctaaaaagct atagaaatcc
     3841 gttacatagt tatcataatc ataagggtca gaacgccatt tatagttgtc ctgattaata
     3901 attctttcta ttattatgcg caatgcagct actatatcaa caagagggtc aggattgatt
     3961 gcaggcatct gttgcaaggc attttttgta ccggtaacat ccgcattgat agcatcgctc
     4021 aacataacat taaacctatg ctggtcaata gtatcaaatg catcatacaa tgccggatag
     4081 ggatctaaaa ggtcaaataa tcggtattcc ggctcaggat cattgcgttg aaacaaaaat
     4141 ttgctactat ccgaacatga taacaacacc gttgcaacgc acatgattat gaagaatgat
     4201 ttaaaaggct tcatggcgca cctcttgtga tttatttaaa aaaaatcatt ataggcgata
     4261 gttactctat aataaaattt aatagtactg acatgtcggt atatttcaac aaaaaaccga
     4321 ctacccagta ctattttccc caataaaaaa ctctttttct ttagccccat ttttgataat
     4381 accttatgga tcaaatacat gctctcctcc cttcctcatt cctgataact attatgacta
     4441 ctttaccgtc cagctatact catattctgt aaacagtata ataccgactg ttaagtattg
     4501 tcaactatta atcaaaaatt ttacaatttt tttaatattt tgtaataata taatattttt
     4561 cacatattat attgtaatta ttatattatt taatatatct ataaatatgt ttttagtatg
     4621 tactcaattt aatatataat taataatact gtaatatagt tatagataac tatattatgt
     4681 aaaagtatat agctaattat tggtataaat ttatgtagtt ttgtcgatat tcaatcttga
     4741 ttaatgcaca tcattaaact ttgtaatcat tattattcct ttaacacacc aatcttcttt
     4801 aactatttgt taaaactttc aaaatctcta tcccacgcct ggtaaattat aattagaacc
     4861 attatcattc ctaactataa actaacaaaa aggctggttt tgtgtggaat ctcctttttt
     4921 acttttaatt ctgatagtat aatacaatcc ctaatggaat aagaacagtt gaaataattc
     4981 attgtacaat catagcacct ttacacagtt ctccttcact ttcatcccct atggatgcca
     5041 gcatttcggg tatggccaac cttcccgaaa atagaaatat acacccatat cataatggac
     5101 tcgtttctgc atctcttatt tcatagttat tgaggctctg accccctttt ataataacta
     5161 tcgcctatta accgtatact atgcctttga cagacaaacg atttattaca tgagtgagta
     5221 cttagtcata attgagtgaa tgttcactca attttgccta ttaatattat ataaattaat
     5281 atcatatttt tattcaacat ttataataat attattgact ttattaatat ttttttgtat
     5341 tgttaaaagc aaagtgtatc aataaacatt tttatttatg cttccaacat gggttttgtg
     5401 aaggatggct ttattgctat gccttaaata tttttaaaag gaggttccct atggatcttt
     5461 ttgataaatg tttagccttc actcgagttg aagaggttaa atcatttgga ttgtatccct
     5521 attaccaccc aataacaaaa acagaaggac ctgttgtgta catgaacaat aaacgtatga
     5581 tcatggtcgg ttcaaataac tatctgggac taacgcatga cccacgagta aaacaggcag
     5641 ctatcgatgc cattgaaaaa tatggcacgg gatgctcagg ttcaagactc atcaatggtt
     5701 cacttgatat tcattgtaag cttgaggaag cgctggcgca atttgttggc aaagaagctg
     5761 cctgtatttt ttctaccggt tatcaaacga atcagggagc tattgtatca ttaataaaca
     5821 aaggggaata tattatttca gatgaagaaa atcatgcaag catcatacag ggcacattga
     5881 tagcaaaagc cctatgtggc gatagtattc tggttaagtt tcatcacaat gatatggttg
     5941 accttgaaaa taaaatctct gctctgccgc atgatgcagg aaaacttatc gttgttgatg
     6001 gagtcttcag catgaatgga cacattgctc ccctcccgga tattgttaga atagcccgta
     6061 aatataatgc gcgcatcatg accgacgatg cacatgctct tggtgttatt ggccatgatg
     6121 gacgcggtac agcaagctat tttggattaa cagaccaaac tgatattatt atgggaacat
     6181 tttcaaaaag tttagcttca tcaggaggtt ttttagcatc aagcgagcgg gttataaact
     6241 acatcaagca taacgcacct gcaattatat ttaatgcaag catgcctgca tcaaatgctg
     6301 ctgcagcact taaagcactt gaaataattc aaaacgaacc tgaatctata caaaagcttc
     6361 ataataacgc aaaacgtttg cggaatggac ttgaacatct tggatttaat gtaataaaag
     6421 gggaaacacc tataatccct gttattattg gtgatgatat catgacgttt acgttatgga
     6481 aaaagctctt tgattatggc gtatatacca atccaataat ctttcctgcg acacctcagg
     6541 ggcagcagtt gatccgctgc agcgtcatgg caacacatac cgatgaacat attgattatg
     6601 tattagggat gtttgaaaaa gctggtgctg aattaaaatt attgcatgca aaggtataat
     6661 caaaattttt tatggctttt tgaaatcaat atagcatcga catcaaaaac aaagcagatg
     6721 ttaccagtgg aagtaggatc agctttttga tgcaatgctg taaggctctg accccttttt
     6781 tatacccact gtgcaatgga gtagcgtttg cgatacgctt cctcatactg caaaaaacgc
     6841 cttacccgtt cgctaaagct gtcccccaac tgcagaaagt actcatgatg gtctatcggt
     6901 acaccaacat cagcaccctc ctgtacatag cattgtatgc tgctgtagcg atattttgta
     6961 ggactatcgc accggcgctt ccgtac
//
LOCUS       DAOSOX010000121         6933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_10981_length_6933_cov_8.169090, whole genome shotgun sequence.
ACCESSION   DAOSOX010000121 DAOSOX010000000
VERSION     DAOSOX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10981_length_6933_cov_8.169090"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLX22_08855"
     CDS             complement(<1..209)
                     /locus_tag="PLX22_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20043.1"
                     /translation="MGLIAILLLCVFYLGLIIIIYEYYTLFSSKDEYTKQELKGVITG
                     APDHWMPLLSQFRLFPVYALASLIVG"
     gene            complement(728..1336)
                     /gene="purN"
                     /locus_tag="PLX22_08860"
     CDS             complement(728..1336)
                     /gene="purN"
                     /locus_tag="PLX22_08860"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOT20044.1"
                     /translation="MKKTVSFLVSGRGSNFQAVAKAIIKGTIPAKTGVVISDTEGVRA
                     LDIAQELSIPAFFINPGQYKSRQEHEKAMVQLLHEYSTDVVVTAGYMRLLTPYFVHEF
                     KNSIINIHPALLPAFPGTHAQRQALEYGVKISGCTTHFIDEGTDSGPIILQKAVPVYD
                     DDTEDTLSARILKEEHQLLIESVRLFCKDKIFIKGRKVYIVR"
     gene            complement(1358..>1673)
                     /locus_tag="PLX22_08865"
     CDS             complement(1358..>1673)
                     /locus_tag="PLX22_08865"
                     /inference="COORDINATES: protein motif:HMM:NF037782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20045.1"
                     /translation="IGRHYRLHDKASIIVARNEEECIILDRYKQHADFYVEPDFKGPV
                     MYGRGTITQNDYLLLTQIMARYGRPTMTENSIALLIQGQRISNLMVDKPIGDALLNQM
                     RI"
     assembly_gap    1674..1773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1774..2201)
                     /locus_tag="PLX22_08870"
     CDS             complement(<1774..2201)
                     /locus_tag="PLX22_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOT20046.1"
                     /translation="MKAKAIALYSGGLDSLLAAAIVARQGVEVIGLHCILPSVDPQKP
                     PDSLAAAQLAKQIDLPLEYYRCDEQYMQVIAHPVHGYGKRANPCIDCKIFFLQKAKEY
                     MQRHNASFVITGDVVGQRPMSQYKAMLRHIEKESGLEGILL"
     gene            complement(2436..>3989)
                     /locus_tag="PLX22_08875"
     CDS             complement(2436..>3989)
                     /locus_tag="PLX22_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HOT20047.1"
                     /translation="KTIIRINYPDEDPRFSPWKERAAKFDFKSIVAIPIIVDDNTHAI
                     GAITLYSRNEHFVTLGEAALLQEVANDISFAIDHIRKNKKIEKILFYDELTGLGNYNY
                     FLSALTSQIQLASSQTMQLAVVTIDIDNFTAINNSFGFNTGDAILREYRNRLQNSIDA
                     NLIARDGDEFFILYPYIDEMGLTSFISDLKLIATKPLIAMSHELVISYCIGIATYPND
                     GKTAEELLNKSRIALTEAKKKGSSSICYYSEELFQNSIQKIALEKELRSALKNNEFTL
                     YYQPKIAVASRNISGLEALLRWNHPEKGLLPPSDFIAILESSDMIIQIGKWVAAEVAR
                     QVQINPMFQEKSFIMSFNVSPNQFYDSAFIFDIIKIVKSSAINPCNLEIEVTENILMS
                     DAENVIDTCNKLQQFGINISIDDFGTGYSSLSYLKKMPVSTLKIDASFVNGLPHNKDD
                     VEIVKTIILLAHSLGKKTIAEGVETREQLDVLVDLGVDEVQGYYFSKPMPIHECVDYC
                     KNFNPDNYF"
     assembly_gap    3990..4089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4090..4640)
                     /locus_tag="PLX22_08880"
     CDS             complement(<4090..4640)
                     /locus_tag="PLX22_08880"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HOT20048.1"
                     /translation="MSIVEKLAQVIAHDMHLDEKTINELHDLFTISDDDTKAIEKIQE
                     IIKLSPDFWKNFYSRCLNDTALQKINSDSQFFKKHKRAIIDYFIKLPEQKIDYDYFIS
                     RFAIGIQLEKATVEPILFANILSNLYIQLIQYCEDIIHDTSLLTEIMQSYLKIVFLDL
                     TLILHAYFFMKEKKLSDAKKELER"
     gene            complement(5045..6757)
                     /locus_tag="PLX22_08885"
     CDS             complement(5045..6757)
                     /locus_tag="PLX22_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOT20049.1"
                     /translation="MAVIDYIIIVIYFLFSIGIAFLFSRRARTNTRQFFLSGQSLPWW
                     ITGTAMVATTFAADTPLAVTELVHSGGIAGNWLWWNMVAANVVTVFFFAKLWRRSGVL
                     TDIEFIELRYSGKEASFLRGFKALYLGLFANCIIMGWVNVALAQILAHLFNINHSYVL
                     FIVIATMLFVGLYSAIGGLWAVAVTDFVQFIIAMGGCIVLAVVVIDHPHIQGIDGLIQ
                     SIPQNALLFFPSGDSSSLPLSAFLLFIGVQWWASWYPGSEPGGGGYVAQRMMSSKDET
                     HSLKATLWFTIAHFALRPWPWIIVALATMVLYPTIPADQSKATYVFAIRDYLPAGLKG
                     MLVAAFLAAYMSTIATHLNWGSSYLVNDLYRRFIVTGKSEKHYVYSAQVCTTLLAVIA
                     SLLIFVIDSISGAWTFIIECGAGIGPVLMLRWYWWRINAWSEIIAMIAPIPAVIISRL
                     VLHIALPYNLLIIVPWTTICWVMATLVTQPVKHETLTAFYHKVQPEFGFEPIRKALHY
                     PPGNYQTCMILLFQTTLGIIATYSLLFGIGAVLLHKTGAYLYLIAFVVSFMLVIYSFR
                     MTKK"
BASE COUNT         2027 a         1354 c         1350 g         2002 t
ORIGIN      
        1 ccaacgataa gtgatgccag tgcataaaca gggaataatc tgaattgtga cagtaatggc
       61 atccagtggt caggagcgcc agtgataaca ccttttagtt cctgttttgt atattcatct
      121 tttgatgaaa ataacgtata gtattcatag ataattatga ttaatcctag ataaaacaca
      181 cataataaaa gtattgcaat taaccccatg tgattcctcc tcaaaaaatt aaattatgca
      241 ttcctgtcat tccgtgctta acacagaatc tattgtgccg ctaccgtcat tctggacttg
      301 ttccggaatc cagatgtgca acgttagatc ctgaaaccag ttcagaacat ggttcaggat
      361 gacactccgc acattgtcat tctgagggga gaagccccga agaatcaatt ggttcacaaa
      421 acaacgagat tctttgcctt gctcaggatg acggtcattc accaaacgat gagaacctcc
      481 gcctttggct caggatgaca gccacataaa acggcgagat tgttcacttc gttcagaatg
      541 acgtgtcgcc caacacagac gccatcattc accatgacag tggtgttaca tagtaacact
      601 atttgtgcgg ctacagagca actatgcatt atcccggaat acataaaata atatcgtaat
      661 aaaaaaatac tgattagtgt aaattttgca agtatttttt taacccggaa agcttattca
      721 gcagtaacta tcgcacaata taaacttttc tgcctttaat aaaaatctta tccttgcaga
      781 aaagccgtac tgattcaatc aatagttgat gctcttcctt gaggatgcgc gccgataagg
      841 tatcctcagt atcatcgtca taaacaggga cagctttttg caaaataatt ggacctgaat
      901 cggtaccctc atcaataaag tgcgttgtgc atccagaaat ctttacacca tattccagcg
      961 cctgcctttg tgcatgtgta ccgggaaatg ctggcagaag agcaggatgt atattgataa
     1021 tgctattttt aaattcatgt acaaaatacg gtgttaaaag gcgcatgtat cctgctgtaa
     1081 cgacgacatc ggtactgtat tcatgcaaca attgtaccat agctttttca tgttcctggc
     1141 ggcttttgta ctgcccgggg ttaataaaaa aagccgggat agatagctcc tgtgcaatgt
     1201 caagggctct gaccccttcg gtatcagaaa taacaacccc cgtttttgca ggtatggtgc
     1261 cctttataat ggcttttgca acagcttgaa agtttgaacc acgtcctgaa actaaaaatg
     1321 atacagtttt tttcattgta ttactccggt gccataatta tatccgcatt tgatttaaca
     1381 gagcatcacc tatgggttta tcaaccataa gattacttat acgctgtccc tgtattaata
     1441 gtgcaatgct attttctgtc attgttgggc ggccatagcg agccataatc tgtgtaagca
     1501 gtagatagtc gttttgcgta atggttcccc tgccatacat gacaggacct ttaaaatctg
     1561 gttcaacata aaaatcagca tgctgtttat acctgtcaag tattatgcat tcttcttcat
     1621 tgcgggctac tatgatactt gctttatcat gtaaccgata gtgcctccca atgnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcagta tcccttcaag cccgctttct
     1801 ttttctatat gtcgcaacat tgctttgtac tgtgacatgg gcctttgacc aaccacatca
     1861 ccggtaatga caaatgaagc attatgcctt tgcatatact cctttgcttt ttgtaaaaag
     1921 aaaattttac agtcaataca tggatttgca cgtttaccat agccatgtac tggatgtgca
     1981 ataacctgca tatactgttc gtcgcagcga tagtattcca gtggaagatc aatctgcttt
     2041 gccagttgtg cagccgcaag gctatcggga ggtttttggg ggtcaactga tggcaaaatg
     2101 cagtgcagtc caataacctc aactccctga cgagcaacta tcgctgcagc aagcaggcta
     2161 tccagtccac cagaatacag ggctattgct tttgccttca tggtgttatt cttcatagtg
     2221 atgaaaaagt tttgccattg ctgctactgc tacctgcatg gcatttacct atactgggaa
     2281 tcatgggttt ttccttgaaa gcttgagtgt atcattactt ctatataaaa agggtatgca
     2341 tggcaatgaa agtcaaggaa ttattttatt actatgatgt gcctgatagc aaagtaccag
     2401 gatggatact gcactattac tggatattgt ttttgttaaa aataattgtc gggattaaaa
     2461 tttttacaat aatcaacaca ttcatggatg ggcattggtt ttgaaaaata atatccctga
     2521 acctcatcaa cccctaaatc tacaagaaca tctaattgtt ccctggtttc aactccttca
     2581 gcgatggttt ttttacccag tgagtgggct aaaaggatga tggttttaac aatttcaaca
     2641 tcatctttgt tatgcggcaa gccattgaca aacgatgcat ctatctttaa agtagatacc
     2701 ggcatctttt taagatatga tagtgacgaa taccctgttc caaaatcatc tattgagatg
     2761 ttaatgccaa attgttgtaa tttattgcag gtatcaatga cattctctgc atccgacatt
     2821 aaaatatttt cagttacttc tatttccaaa ttgcagggat taatagccga gcttttaact
     2881 attttgatta tatcaaatat aaatgctgaa tcataaaatt gattggggga tacgttaaat
     2941 gacatgataa aacttttttc ctgaaacatt ggattaatct gcacctgcct tgccacctct
     3001 gctgcaaccc atttccctat ctggattatc atatcagatg attccagaat agcaataaaa
     3061 tcagatggtg gcagcaagcc tttttccggg tgattccatc gtaacagcgc ttccagccct
     3121 gaaatgttac gcgacgctac agctattttt ggctgatagt aaagggtaaa ttcattgttt
     3181 ttgagtgcac tgcgcaattc cttttccaga gcaatttttt gaatgctatt ttgaaaaagt
     3241 tcctctgaat agtagcatat actgctgctt cccttctttt ttgcctcggt cagagcaatc
     3301 cttgatttat taagaagctc ctcagcagtt ttgccatcgt ttggataggt tgcaataccg
     3361 atgcagtacg agataaccag ttcatggctc atagcaatta atggttttgt ggctatcagt
     3421 ttaaggtcgc tgataaagga tgtcagcccc atctcatcaa tgtaaggata aagtatgaag
     3481 aactcatcgc catcgcgtgc aataagattt gcatctatac tattttgaag tcggttgcga
     3541 tattcacgca gtatagcatc gcctgtgttg aagccaaatg agttattgat ggcagtaaag
     3601 ttatcaatat caatagtaac aacggccaat tgcatcgttt gtgatgaagc taactgtatc
     3661 tgtgaggtca gggcagataa aaagtaattg taattcccca gaccggtaag ctcatcataa
     3721 aaaaggatct tttcaatctt tttatttttt cgtatgtggt ctattgcaaa ggagatatca
     3781 ttggcaacct cttgcagaag tgccgcttca cccagcgtga caaagtgttc attacgtgaa
     3841 tatagagtaa tggcgccaat tgcatgggta ttgtcatcaa cgatgatggg tattgctaca
     3901 atggatttaa aatcaaattt ggcggctcgt tccttccacg ggctaaagcg cggatcttca
     3961 tcggggtagt taatcctgat tatggttttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnc gctccagttc cttttttgca tcggacagct ttttttcctt cataaaaaaa
     4141 taggcatgca gtatcaatgt taaatctaaa aaaacaattt ttaagtatga ctgcattatc
     4201 tctgtaagta gtgatgtatc atgaattata tcctcacaat actgtatgag ctgtatatac
     4261 aaattgctca agatgtttgc aaacagaatt ggctctacag tagctttttc aagctgtatg
     4321 cctatggcaa agcggctaat gaaataatca taatcaattt tttgttccgg taattttata
     4381 aaatagtcta taatggcacg tttgtgcttt ttaaaaaatt gtgaatcaga attaattttt
     4441 tgcaatgcag tatcgtttaa acaccggcta tagaaatttt tccagaaatc aggtgacaat
     4501 tttatgattt cctgtatttt ttctattgcc tttgtatcgt catcgcttat ggtaaacaga
     4561 tcgtgcagct cgttgatagt tttttcatca aggtgcatat catgggcaat aacttgtgct
     4621 aatttttcaa caatgctcat ggttaaattt aattccgtcc agacttatgg taaaatttat
     4681 acaaaataaa gtataaagga tcaataaaaa aatagcgcgc tcaggaatag taaaaaaaaa
     4741 tattttgata gttgctttac aaaaataaaa atttttgcaa tgattacatt ctatccataa
     4801 atgtaatgga atcataataa cgatatccct tttcgtcatt ctgaggggcg aatcccagaa
     4861 gaatatattc gttcacaaaa cgacaagatt cttcactccg ctcagaatgc cagcggcaca
     4921 caaaacggtg ggattcttca cctggttcag attgacaggc gataagcttg gattgacacg
     4981 gtgccttgtt actttttaac aaagcaggta cactatacag taactatcgc acataatgat
     5041 gttattattt ttttgtcatg cgaaaagaat agatgaccag cataaagctg acgacaaatg
     5101 caatgagata gaggtatgct cctgttttgt gcagcaacac tgcccctatg ccaaagagca
     5161 gcgagtaggt ggctatgatg ccaagtgtgg tctggaataa aagtatcata catgtttgat
     5221 agttacccgg aggataatgg agcgctttgc gtatgggctc aaacccaaat tcaggctgta
     5281 ccttgtgata aaatgctgtc agcgtttcat gtttgacagg ttgggttaca agggttgcca
     5341 tcacccagca gattgtggtc catggaacga tgatgagcag attatagggt aaggcaatat
     5401 gcagtaccag ccgcgatata ataaccgcgg ggatgggtgc tatcatggct ataatctccg
     5461 accatgcatt gatacgccac cagtaccagc gcaacatgag caccggccct atgcctgcac
     5521 cgcattcaat aatgaatgtc catgctccgg atattgaatc gataacaaag attaacaggc
     5581 ttgcaataac tgccagcaat gtggtgcaca cctgtgcgct gtaaacatag tgcttttcac
     5641 tttttcccgt tactataaat cgtcggtaca gatcattcac aaggtacgag cttccccagt
     5701 ttaaatgggt ggcaatggtt gacatgtatg ccgctaagaa tgctgcaaca agcataccct
     5761 ttaagccggc aggcagataa tcgcgtattg caaaaacata ggtggctttg gattggtcag
     5821 cggggatagt gggatacagc accatggttg caagggcaac gattatccat ggccatggtc
     5881 gtaaggcaaa atgtgctatg gtaaaccaca gtgttgcttt taatgagtgt gtttcatcct
     5941 tagaagacat catacgctgt gcaacatagc cgccaccgcc tggctctgac cccggatacc
     6001 acgatgccca ccactgaaca ccaataaaca acaaaaaggc agagagcggc agggaggaac
     6061 tatcgccaga ggggaaaaat aaaagagcat tttggggtat ggactgaatt aaaccatcga
     6121 tgccctgtat atgcggatga tcaatgacaa ccactgcaag cacaatacat ccacccatag
     6181 caataatgaa ctgaacaaaa tccgtaacag caacagccca taagccgcct attgcagaat
     6241 agagtcccac aaagagcatt gttgcaatga caatgaacag tacgtatgaa tggtttatgt
     6301 taaaaagatg tgctaaaatc tgtgccagag caacgtttac ccagcccata atgatacagt
     6361 tggcaaaaag tcccagatat aatgccttga atcctcgtaa aaatgatgcc tctttgccac
     6421 tgtagcgcag ctcaatgaac tcaatatcag ttaacacacc agagcgtcgc cataattttg
     6481 caaagaaaaa cacggtaaca acattagcgg caaccatatt ccaccatagc cagttacctg
     6541 caatacctcc gctgtggaca agctcggtta ccgcaagggg ggtatcggct gcaaatgttg
     6601 ttgccaccat ggctgtaccg gttatccacc atggaaggct ttgaccggaa aggaagaatt
     6661 gcctggtgtt agtgcgcgcc cgtcgggaaa aaagaaaagc aatgccaata ctgaacagaa
     6721 aatatataac gatgatgata taatctatga cagccattat tggtgcaata gatctttata
     6781 gtaaaagtca aggtcaaaac cgccaaaccg tgattcaaat gaaagattta tattttcata
     6841 gagtgttttt aaagtggtat gttcttcgtt gtattcaggt ggtgatggtt tgagattatg
     6901 ctttataaac tgaagctgat ttataaatgt tcc
//
LOCUS       DAOSOX010000122         6909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11021_length_6909_cov_6.192588, whole genome shotgun sequence.
ACCESSION   DAOSOX010000122 DAOSOX010000000
VERSION     DAOSOX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11021_length_6909_cov_6.192588"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLX22_08890"
     CDS             <1..303
                     /locus_tag="PLX22_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20050.1"
                     /translation="DYIFDSIQVLISVAGNAGNIFDCMALYKYTVQKQILAMHEFHEL
                     MKSYSMEYLMSMRYNPPMIDAAKLEYQIRLIDSICMVLFGKPAPGYVPDFNDTWND"
     gene            610..4461
                     /gene="cas12a"
                     /locus_tag="PLX22_08895"
     CDS             610..4461
                     /gene="cas12a"
                     /locus_tag="PLX22_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HOT20051.1"
                     /translation="MSNMQQYDNFINHYAIQKTLRFELQPIGKTREHIQKNGIIEHDE
                     ALEQKYQIVKKIIDRFHRKHIDEALSLADFSKDTAMLKRFEELYWKKNKNENEKNEFV
                     KIQSDLRKRVVSFLEGKVEGDARFAKVQQRYGILFDAKIFKDKEFISTACDDIEKDAI
                     EAFKRFATYFTGFHENRKNMYSADEESTAIAYRVINENLPRFLENKARFEKIQHTVDS
                     KTLNEIATELKPVLEKNKLETIFTLNYFQNTLSQAGITYYNTILGGKTKENGEKVQGL
                     NEIINLFNQKNKDTMLPLLKPLYKQILSEEYSTSFTISAFEKDNDVLQAIGSFCNDCI
                     FYAKNNVNGKAYNLLQTVQAFCNSIDTYNDNRLDGLHIERKNLATLSHQVYGEWNILR
                     DALQIHYEAYEQKDNGNNNNYLESKTFSWKALKDALTTYKSLVEEAQDIDENGFIAYF
                     KDMKFKEEIDGKTTSIDLIENIQTRYKSIETILQEDRNNKNNLHQEKEKVATIKGFLD
                     SVKYLQWFLNLMYIASPVDDKDYDFYNELEMYHDTLLPLTTLYNKVRNYMTRKPYSVE
                     KFKLTFEKSTLLDGWDKNKERANLGVILRKGNNYYLGIMNKKYNDIFDSIPGLTTTDY
                     CEKMNYKLLPGPNKMLPKVFFSKKGVQFYKPSQEIIRLYNNKEFKKGDTFNKNSLHKL
                     INFYKESIAKTEDWSVFQFKFKNTNDYADISQFYKDVERQGYKISFDKIDWEYILLLV
                     DEGKLFLFKIYNKDFSPYSKGKPNLHTIYWKNIFSHDNLNNVVYKLNGEAEVFYRKKS
                     IEYPEEILQKGHHVNELKDKFKYPIIKDKRYAEDKFLFHVPITMNFLSKGEPNINQRV
                     QQYIASTSEDYHIIGIDRGERNLLYLSLIDATGKIIKQLSLNTIKNENFNTTIDYHAK
                     LDEKEKKREEARKNWDVIENIKELKEGYLSQVVHQIAKLMVEYKAILVMEDLNTGFKR
                     GRFKVEKQVYQKFEKMMIDKLNYLVLKDRQATQPGGSLKAYQLASSLESFKKLGKQCG
                     MIFYVPAVYTSKIDPTTGFYNFLRVDVSTLNSAHSFFNRFNAIVYNNEQDYFEFHCTY
                     KNFVSEPSLQKNVKSSKMHEYNNLKDTTWVLCSTHHERYKKFKNKSGYFEYKPVNVTQ
                     SLKQLFDEAGIDYQAGADLKEAIVTGKNTKLLKGLGEQLNILLAMRYNNGKHGNEEKD
                     YIVSPVKNNYGKFFCTLDGDASLPVDADANGAYAIALKGLMLVERMKSNKDIKGRIDY
                     FISNNEWFNYLIAKNTLNKSK"
     gene            4480..5040
                     /gene="cas4"
                     /locus_tag="PLX22_08900"
     CDS             4480..5040
                     /gene="cas4"
                     /locus_tag="PLX22_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HOT20052.1"
                     /translation="MEPYIPISMLNDFIFCPRSIYFHQVHGGMEQDMYHTEVQIEGKA
                     AHQSIEKKYYSTQKDVLIGIDVYSEKYNVAGKIDIFDVKACKLVERKNKVVTIYDGYV
                     FQVYAQTFALREMGYEVKKIVIHDKTHNKNYPIPLPEDDDAMLNKFEKLIDDVNSFDL
                     HDTGFNANIEKCKRCIYSHLCDYCLC"
     gene            5034..5999
                     /gene="cas1"
                     /locus_tag="PLX22_08905"
     CDS             5034..5999
                     /gene="cas1"
                     /locus_tag="PLX22_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HOT20053.1"
                     /translation="MLSLPDFEEKSIIVCFTTEGQTVSFKNDNVVIKDKKGKTILQHS
                     CYRLFSLWIVGHTTITSGILQRSKKFGFSIYLFSYSFKITGVWNSCAEGNFLLREKQY
                     SYNNLDIAWHIVTNKIKNQIALLKSIRDKEEEQKSSIANLDVYLHHTVEPLDLKMILG
                     IEGITTKLFFKEWFTNIAWNGRKPRTKIDTTNTLLDIGYTFLFNIMDAMLNLYGFDVY
                     RGVYHRNFYQRKSLVCDMVEPFRCIIDKKIKNAYNLGQIKAADFTESRGQYFLNHDKN
                     KEYTGWLLQEIMKYKKDMFVYVQKYYRAFMKEKPIKDYPEFLIKE"
     gene            6007..6279
                     /gene="cas2"
                     /locus_tag="PLX22_08910"
     CDS             6007..6279
                     /gene="cas2"
                     /locus_tag="PLX22_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOT20054.1"
                     /translation="MLLVSYDISNTKLRTKFSKFLEKYGERIQYSVFEIKNSERVLKN
                     IETEIKQYFEKKFTQSDSILIFHLSEKCTITRYGYAKNRESDILIV"
     repeat_region   6435..6851
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6435..6470
                     /rpt_unit_seq="gtctaaaggtattataaaatttctactattgtagat"
BASE COUNT         2611 a          959 c         1244 g         2095 t
ORIGIN      
        1 gattatatat ttgatagtat tcaagtattg attagtgttg ccgggaatgc tggtaacatt
       61 tttgattgta tggcactgta taagtacaca gtacaaaaac agatactggc tatgcatgaa
      121 tttcatgaac ttatgaaatc atacagcatg gaatatctca tgtccatgag atataaccct
      181 cccatgatag acgcagcaaa gcttgagtat cagatacgct taatagacag tatatgcatg
      241 gtgctgtttg gaaagccagc gccagggtat gtgccggatt ttaatgatac atggaacgat
      301 tgaaaaatac acaatgctta cgataattaa gggattgata agctttgctt tgtattacaa
      361 cataaatgct aataaatgca gtataaaaat agttactgga gggacattat atgtcacagt
      421 agttatgtat attaacaatg aatatggatt ttttattaaa ataagttgct ttatgcaagc
      481 actgcgtgct acaactaatg aatgctaaaa ccccggagaa ggggtattag cggataatga
      541 ttattattca aacccggtca ggggtgatga aatgatattg tgcatcattt tatttttgga
      601 ggaataacaa tgagtaacat gcaacagtac gataatttta tcaatcatta tgcaatacag
      661 aaaacactac ggtttgaact gcaaccaata ggaaaaacac gggagcatat tcaaaaaaac
      721 gggattattg agcatgatga agcgcttgag caaaagtatc agattgttaa aaagattatt
      781 gatcgttttc acaggaaaca cattgatgaa gcactttctt tagcagattt ttcaaaggat
      841 actgctatgt taaagcgatt tgaggaatta tattggaaaa agaacaaaaa tgaaaatgaa
      901 aaaaatgaat ttgtaaaaat acaatctgat ttgcgcaaaa gggttgtttc ttttttagaa
      961 ggtaaagtag aaggtgatgc aaggtttgca aaggtacagc aacggtacgg tatactattt
     1021 gatgcaaaaa tatttaaaga caaagaattt atatcaacag cctgtgatga tattgaaaaa
     1081 gatgctatag aagcttttaa acgatttgct acctatttta caggtttcca tgaaaatcgt
     1141 aaaaacatgt attccgctga tgaagaatct actgccattg cctatagagt aatcaatgaa
     1201 aaccttccgc gttttttaga aaataaagca aggtttgaaa agatacaaca cacagttgat
     1261 agtaaaacgc ttaacgagat agcaacagaa cttaaaccag tattggaaaa gaataagctt
     1321 gaaaccatat ttacgctgaa ctattttcaa aatacactat cgcaggctgg aattacctat
     1381 tataacacca tccttggtgg taaaactaaa gaaaatggcg aaaaggttca gggcttaaat
     1441 gaaattataa atctttttaa tcagaaaaat aaggatacta tgctgccatt attaaaacca
     1501 ctgtataagc aaattttaag tgaagagtat tccacatcat ttaccatttc tgcttttgaa
     1561 aaagataatg atgttttgca ggcaataggt tctttttgta atgattgtat tttttatgca
     1621 aaaaataatg tcaatggcaa ggcatacaac cttttgcaaa cggtacaggc attttgtaac
     1681 agtattgata cctataacga taacaggctt gatggtttgc atatagaaag aaaaaacctt
     1741 gctacccttt cacatcaggt gtatggcgaa tggaatatac tgcgggatgc attacagata
     1801 cactatgaag cgtatgaaca aaaggataat ggcaataata ataactatct ggaaagcaaa
     1861 accttttcgt ggaaagcatt aaaagatgca ttaacaacat ataaatcact ggtggaggaa
     1921 gcacaggata ttgatgaaaa tggatttata gcttacttca aggacatgaa atttaaagaa
     1981 gaaattgatg gtaaaacaac cagtattgat ttaattgaaa atatacaaac ccggtataaa
     2041 agtattgaaa ccatattgca ggaagaccgc aacaacaaaa acaatcttca tcaggaaaag
     2101 gaaaaggtag ctacaataaa gggctttctt gattcggtaa aatacctgca atggttttta
     2161 aaccttatgt atatagcaag ccctgttgat gataaagatt atgattttta caatgagctt
     2221 gagatgtacc atgacacatt gttaccactt actacactgt ataataaagt acgcaactat
     2281 atgacacgca aaccatattc cgttgaaaag tttaaattga cctttgaaaa atcaactctt
     2341 cttgatggat gggataaaaa caaggaacga gccaatcttg gtgttatact aaggaagggt
     2401 aataattatt atcttggcat tatgaacaaa aaatacaatg atatatttga tagtatcccc
     2461 ggacttacta ccactgacta ttgtgaaaag atgaactata aattgctgcc tggccccaat
     2521 aaaatgttgc ctaaagtatt tttttcaaaa aaaggtgtac agttttacaa accttcacag
     2581 gaaatcatcc gtttgtataa taataaggaa tttaaaaagg gagatacctt taataaaaat
     2641 agtttgcaca aacttattaa tttttataaa gaatccattg caaaaactga agattggagt
     2701 gtattccagt ttaaatttaa aaacacaaac gattatgccg atataagcca gttttataaa
     2761 gatgttgaaa gacagggcta taaaattagt tttgataaga ttgattggga atatatacta
     2821 ttgctggtag atgaaggaaa attatttctt ttcaagattt acaataagga cttttcaccc
     2881 tatagcaagg gtaagcctaa cctgcatacc atttactgga aaaatatttt ttcccatgat
     2941 aatttaaaca atgttgtgta taagcttaat ggtgaagccg aagtttttta ccggaagaaa
     3001 tccatagagt atccagaaga aatactacaa aaaggtcatc atgtcaatga gttaaaggat
     3061 aaatttaagt accctattat taaagataaa cgctatgccg aagacaaatt tcttttccac
     3121 gttcccatta ccatgaactt tttatcaaaa ggggaaccta atataaatca aagggttcag
     3181 caatatattg catctactag tgaagattat catattattg gtattgaccg gggtgaaaga
     3241 aatttactgt atctgtcgct catagatgca acaggcaaaa taataaaaca actatcgctc
     3301 aataccatta aaaatgaaaa ttttaacaca acaatagatt accatgcaaa attagatgaa
     3361 aaagaaaaga aaagagaaga agcaagaaaa aattgggatg tcatagaaaa tattaaagag
     3421 cttaaagaag gatacctgtc acaggttgtt catcagattg caaaacttat ggttgaatat
     3481 aaagcaatcc tggtgatgga ggatttgaat accggtttta agcgtggaag gtttaaagtg
     3541 gaaaaacagg tatatcaaaa atttgaaaaa atgatgatag ataaattaaa ttaccttgtg
     3601 ttgaaggaca ggcaggctac acagccaggt ggaagcttaa aagcatatca acttgcatcg
     3661 tcattggaat cgtttaaaaa acttggtaaa cagtgtggca tgatttttta tgttcctgca
     3721 gtgtatacaa gcaagattga tccaacaact ggtttttaca actttttaag ggtagatgtt
     3781 tcaacattga acagcgcgca cagttttttt aacaggttta atgcaattgt ttataataat
     3841 gagcaggatt attttgaatt tcattgcacc tacaaaaatt ttgtttctga accgtcattg
     3901 caaaaaaatg taaaaagcag caaaatgcac gagtataaca atttaaaaga tactacatgg
     3961 gtactatgct ctactcatca tgagcgctat aaaaagttta aaaataaaag cggttatttt
     4021 gagtataaac cagttaatgt aacccaaagc ttaaagcagc tttttgacga ggctggtatt
     4081 gattatcaag caggggctga cttaaaggaa gcgatagtaa ccggtaaaaa tactaaacta
     4141 ttaaagggtc tgggcgagca gttgaacata ctattagcca tgcgatataa caatggtaaa
     4201 catgggaatg aagaaaaaga ttatattgtt tctccagtaa aaaataatta tggaaaattt
     4261 ttctgcacgc ttgatggtga tgcatcatta cctgttgacg ccgatgccaa tggtgcgtat
     4321 gccatagcat tgaaagggct tatgcttgtt gaaaggatga aaagcaataa agatataaaa
     4381 gggaggatag attattttat ttcaaataat gagtggttta actatttaat tgcaaagaac
     4441 acattaaata aatcaaaata attaatcgaa aatgaatata tggaaccata tattcccatt
     4501 tcgatgctga atgatttcat attctgccca cgctccatat actttcatca ggttcatggt
     4561 ggcatggagc aggatatgta tcatactgag gttcaaattg aagggaaagc agcccaccaa
     4621 tccatagaaa aaaaatatta ttctacacaa aaagatgtac tcattggcat tgatgtatat
     4681 tctgaaaaat ataatgtagc aggcaaaata gatatatttg atgtaaaagc ttgtaaactt
     4741 gttgaacgaa aaaataaagt ggtaacaatt tatgatggtt atgtttttca ggtatatgca
     4801 caaacatttg cattacgcga aatgggctat gaggtaaaaa agattgtaat tcatgataaa
     4861 acgcacaata aaaattatcc cataccattg cccgaagatg atgatgcaat gctcaacaaa
     4921 tttgaaaagc ttatagatga tgtaaattcc tttgacttac atgataccgg atttaacgca
     4981 aacattgaaa aatgtaaacg ctgtatatat tctcatttat gtgattactg cttatgttaa
     5041 gtttaccaga ttttgaagaa aaaagtatta ttgtatgctt tacaacagaa gggcaaacgg
     5101 tttcatttaa aaatgataat gtggttataa aggataaaaa agggaaaacc attttgcagc
     5161 actcgtgcta tagactgttt tcgctatgga ttgttgggca tacaactatt acatctggta
     5221 ttcttcagcg cagtaaaaaa tttgggtttt caatctattt gttttcatat agttttaaaa
     5281 ttaccggtgt gtggaattcg tgtgcggaag gtaatttttt actacgcgaa aaacaataca
     5341 gttataataa tctggatatt gcatggcaca tagtaacaaa taaaataaaa aaccagatag
     5401 cattattaaa aagtatacgc gataaagaag aagaacaaaa aagctccatt gccaatttag
     5461 atgtgtattt acaccataca gtagagccat tagatttaaa aatgattttg gggattgaag
     5521 gcattaccac taaattattt tttaaggagt ggtttaccaa tattgcatgg aatggaagaa
     5581 aaccgcgtac caaaattgat acaacaaata cactgttaga tattggctac acatttttgt
     5641 ttaatatcat ggatgcaatg ctcaacctct atggctttga tgtttataga ggtgtatatc
     5701 acagaaattt ttatcaacga aaatcactgg tatgtgacat ggtagagcca tttagatgta
     5761 ttatcgataa aaaaattaaa aatgcgtata acctgggaca aatcaaagct gctgatttta
     5821 ctgaaagcag ggggcaatat tttttaaacc atgataaaaa caaagaatat accggctggc
     5881 tattacagga gatcatgaag tataaaaagg atatgtttgt gtatgttcaa aaatactata
     5941 gagcttttat gaaagaaaag ccaataaagg attatcctga atttttgata aaggaataaa
     6001 ataacaatgc tacttgtttc gtatgacata agcaatacaa agttgcgaac taaattttca
     6061 aagtttttgg aaaaatatgg ggaaaggata cagtactcag tttttgaaat aaaaaacagt
     6121 gaacgcgtgc taaaaaatat tgaaaccgag attaagcagt attttgaaaa aaaatttacg
     6181 caaagcgata gtattttgat atttcatctc agcgaaaaat gtaccataac caggtatgga
     6241 tatgcaaaaa acagggaaag cgacatactc attgtatgat tgcaaacccc cgtcttaatg
     6301 gaataattgc tcatgaagcg ataatttttg atataaaagt tgtaaaaatc agctcatttt
     6361 gtgcacaaag tacacaattt ttaccccaaa aaggcttgta atgataaaat agttgacaaa
     6421 agagcaaatt acctgtctaa aggtattata aaatttctac tattgtagat tattctctaa
     6481 catttgcaca aatgacaagt ctaaaggtat tataaaattt ctactattgt agattcaatg
     6541 ggttctacaa ttaatttaaa agtctaaagg tattataaaa tttctactat tgtagatgtt
     6601 tgtgaaataa ttcagttgct tcatgtctaa aggtattata aaatttctac tattgtagat
     6661 gtttgtgatt aagtggttaa ctaaagaggt ctaaaggtat tataaaattt ctactattgt
     6721 agatatattg acgaagtata ttatttttgt aaagtctaaa ggtattataa aatttctact
     6781 attgtagata tagtaaactc tttcctttct tggaagtcta aaggtattat aaaatttcta
     6841 ctattgtaga tctataactc cttcttcatt tcctaatcgg tctaaaggta ttataaaatt
     6901 tctactatt
//
LOCUS       DAOSOX010000123         6840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11166_length_6840_cov_8.253500, whole genome shotgun sequence.
ACCESSION   DAOSOX010000123 DAOSOX010000000
VERSION     DAOSOX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11166_length_6840_cov_8.253500"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /gene="rbfA"
                     /locus_tag="PLX22_08915"
     CDS             <1..275
                     /gene="rbfA"
                     /locus_tag="PLX22_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT20055.1"
                     /translation="VKMSKDYAYADVYISILGDEHDRQKTLEGLQSARGFVQYRVGKS
                     LRIHTIPEVRFYVDTSIEEGVTMVNLLEKLQKESAKNDNGDSSSKS"
     gene            272..1216
                     /gene="truB"
                     /locus_tag="PLX22_08920"
     CDS             272..1216
                     /gene="truB"
                     /locus_tag="PLX22_08920"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT20056.1"
                     /translation="MKVSYSKGCVLVIDKPAGYTSFDIISIIKKKLKVKKAGHTGTLD
                     KFASGVLVLCTGWTTKLVPYFMDMDKRYIATIQLGIATDTHDCEGAIIATHEVNCTFE
                     EIDSCLQDTFVGTISQTPPLYSAVKIGGRRASDRVRRGEEITIAPRTVCIYAINVLNF
                     DKQRAQITLDVLCSKGTYIRALARDLGNSLQVGAHVVRLQRIAVGHFSVDNALRLEQC
                     DELPGKEIKNIDGVYAPYDALYEFGVVEVKEKALINIKHGKHLTADDVVTATEKNELF
                     RVIPEGQKVLVAIAHKQGEVYRCERVFVEEIDMLDTIL"
     gene            1357..1614
                     /gene="rpsO"
                     /locus_tag="PLX22_08925"
     CDS             1357..1614
                     /gene="rpsO"
                     /locus_tag="PLX22_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT20057.1"
                     /translation="MVEKNEIINKYRVHEKDTGSTEVQIALLTERINHLTEHFKVHIK
                     DHHSRRGLLKLVGTRRRLLDYLKKKDVMKYRELIKSLGIRR"
     gene            1659..3746
                     /locus_tag="PLX22_08930"
     CDS             1659..3746
                     /locus_tag="PLX22_08930"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT20058.1"
                     /translation="MNKEFAVTIGNDLIQFSTGILAKQADGAVVVSCGETIVFSSAVA
                     SKKVVEGQDFFPLTVDYREKHYSAGKIPGGFIKREGRPSDKEILTSRLADRPIRPLFP
                     DDFVNEVQIIIYVLSADREHQPDILAINAASAALSISGVPFKGPVGAVRVGRVDGQWI
                     VNPSFQEMENSDIDLVVAGTRKAVTMIEGQAKNLSEDAMIQAVEFAHANIIKLCEIQE
                     ELKNTCGKPLMQYIPKLSDNKLEKVIREKYFTAVENFKEYTEKKAREDAKDAIITAIT
                     NDLKEQFPDTIALVPEIVDAMDAEVIRSRILDEGSRPDGRGLNNIRPIDIMVGILPRA
                     HGSALFTRGQTQSLGIVTLGTVAESQRIDSIDLLEESYKRFMLHYYFPPFSTGEVGRV
                     GGVGRREIGHGMLAERAIEYAIPDEKEFPYTIRVVSEVLESNGSSSMATVCAGSLALF
                     NAGVPMKDQVAGIAMGLVLEGERYAILSDIQGIEDHLGDMDFKVAGTATGITAFQMDI
                     KIEGITTEIMRKALEQAKEGRLYILEKMNSVVSQPAQELSPHAPKIAVMKIEIEKIGE
                     VIGPGGRVIKKIIEETGAEINIEQDGTIFISANEQSAIDKAQQAIKGLIEEVEVGTIY
                     KGVVKRITDFGAFVEVLPGKQGLVHISKLDFNRVEKVKDVLKEGDEVMVKVIGIDDQG
                     RINLSRKDAMKKH"
     gene            3755..5017
                     /locus_tag="PLX22_08935"
     CDS             3755..5017
                     /locus_tag="PLX22_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT20059.1"
                     /translation="MVHKFTLPDGLVVLLEPIDNVVSISCGLWIRVGSRHEKANEMGY
                     AHFIEHMLFKGTKKYSSKDIARLVDRVGGQHNAATNREYTCYYINVVSDYLELACEIL
                     SDTYFNSIFDAEEIEKEKNVVLEEIRMYEDTPDEHIHDLFMENMFYQSPLGHPILGVV
                     DTVTTINRDSIVEFYKQHYSDDNCLFAISGNFTVDNARTIIEKYFCSNCSPENNAPAV
                     MPQPSKRLYRYHMDKDLEQIHLCIGTDGLSKVDDDRWAYYLFNTILGGSMSSRLFQNI
                     REKEGLCYSIYCFHSSFMDTGIFGIYCGTSPSQYNKLLPLIANECKQIVSQGITQEEL
                     SDAKSYLIGNMALSLESTEVRMSHIAKNEMIYGRHFTFQEIAREIQTVTMDDFMRIAF
                     RIFNNAQFSLVTIGKLPQHHIDTFHLDL"
     gene            5078..6337
                     /locus_tag="PLX22_08940"
     CDS             5078..6337
                     /locus_tag="PLX22_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20060.1"
                     /translation="MSENLQHIYRSLADILPQAHERKIVLITGARQTGKTTLAKKRYA
                     QLKYINLDSPEMRDSIRKVAASDWDKDVGIAVLDEAQKEPTIFEKIKYSYDEMSLPFS
                     VILGSSQILLLKNIRESLAGRIWLYELWPLMMSEMAAGSRRAEKPLLGAILEADSIDT
                     VLTAKPSILIGNENTIKKKAEDYLLQWGGMPALIHLESDNEKHQWLKNYNYTYLERDM
                     IDLSRLYDLEPFQKFQRLSAFRFSSLLNYSELARDAGISTDTARRYLEYLKISYQVLL
                     LQPYSINLTSSMVKTPKIYWIDMGIVRQLTGYYTNIPTGQLYENMVIAEMYKYIKTFH
                     SNANLYFYRTRSGLEVDIVIETPKGLMLAEIKNREDVVTGDYTAIREVANKMGTRFIG
                     GMVIYRGDKIYTLAQSIWAIPSHRLFT"
     gene            6377..>6840
                     /locus_tag="PLX22_08945"
     CDS             6377..>6840
                     /locus_tag="PLX22_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOT20061.1"
                     /translation="MLDAIITSKTRLKILLKFFINPSTKGYLRELSAQMNESTNAVRV
                     ELDRLVEAGYLERNANGNKVYYNANTMHPLYKDIHSVVKKYLGIDVLIDSVLAKLGKV
                     HEAYIVGDYSRGVDSGIIDVVIIGTVDYTYLAQLISKAEAIIKRKIRPLVLSP"
BASE COUNT         2129 a         1197 c         1615 g         1899 t
ORIGIN      
        1 gggtaaagat gagtaaagat tatgcatatg ctgatgtata tatttcaatc ctgggcgatg
       61 aacatgaccg gcaaaagaca ttagaagggc tacaatctgc acgcggattt gtacagtacc
      121 gggtggggaa gtcattacgt atccatacta taccggaagt gaggttttat gtggatacgt
      181 caattgaaga aggcgttacc atggtaaatc tgcttgagaa gctgcaaaaa gaatctgcta
      241 aaaatgataa tggtgatagt tccagtaaat catgaaagtt tcatatagta agggttgcgt
      301 gctggttata gacaaacctg caggatacac ctcttttgat attatcagca taatcaaaaa
      361 aaaattgaag gtaaaaaaag ctggccatac aggaacgctg gataagtttg catcgggtgt
      421 tctggttctg tgtacggggt ggactactaa actggtgccg tattttatgg atatggataa
      481 gcggtatatt gcaaccattc agttagggat tgcaaccgac acccatgatt gtgagggtgc
      541 catcattgca acccatgaag taaactgtac ctttgaagag atagattcat gtctgcagga
      601 tacatttgtt ggtactatca gccagacccc gccgctttac tcagcggtaa aaatcggcgg
      661 aagacgcgct tcagatagag tacgacgggg tgaagagatt actattgccc cgcgaacagt
      721 ctgtatttat gcaatcaatg ttcttaactt tgataagcag cgcgcacaga ttacactgga
      781 tgttttatgc agcaagggaa cctatatcag agcacttgct cgggatttag gtaacagttt
      841 acaggtgggc gctcatgttg tgcggctgca gcgtatagcg gttggacatt tcagtgttga
      901 taatgcttta cggctggagc agtgtgatga actgcctggc aaagaaataa aaaacatcga
      961 cggggtatat gctccctatg acgccttata tgaatttggt gtggttgaag ttaaagaaaa
     1021 agcgcttatt aatattaagc atggtaaaca ccttactgct gatgatgttg ttactgccac
     1081 tgagaaaaat gaactgttca gggtaatccc tgaaggacaa aaggtgcttg tggcgatagc
     1141 tcataaacaa ggggaagtgt accgatgtga aagagttttt gttgaagaaa ttgatatgct
     1201 tgatacaata ctataatagt atatataaaa gtatatataa atttatttct tgactgattt
     1261 tagcttgcaa tagcagtgtt aacagttacg attagactaa ctatgaataa aaagtatttt
     1321 aagtaaagtg ataggtaagg aggagaaaag cgtcgtatgg ttgaaaaaaa tgagattatc
     1381 aacaaatacc gggtgcatga aaaggatacc ggttcaactg aggtacagat tgctctttta
     1441 actgagcgga ttaatcatct tactgagcat tttaaggtac acatcaaaga tcatcattca
     1501 agacgtggat tgcttaagct ggtaggtaca cgaagaaggc tgcttgatta tcttaagaaa
     1561 aaggatgtga tgaagtacag ggaactgatt aaatctcttg gtatacgaag gtagagcaga
     1621 aacaatggta ttacatttta ggtgtgaagg aatgaacaat gaataaggaa tttgcagtaa
     1681 caataggaaa tgatttaatt cagttcagta caggcatttt agcaaagcag gcagatggtg
     1741 cagtagttgt ttcctgtggt gaaaccattg tattttcatc tgctgttgct tcaaaaaagg
     1801 ttgtagaagg gcaggatttt tttccattaa cggttgatta ccgggaaaag cattattcag
     1861 caggcaaaat accgggtgga tttattaagc gtgagggcag accaagcgat aaagagatcc
     1921 ttaccagccg gttggccgac cgtcccatac gtcctctttt cccggatgat tttgtcaacg
     1981 aggtgcagat tattatctat gttttatcag cggacaggga acatcagcct gatatcctgg
     2041 caataaatgc tgcttccgca gcgttaagca tctcaggtgt accgtttaaa ggaccggttg
     2101 gcgctgtaag ggttggaagg gttgatgggc aatggattgt gaatccatct tttcaggaaa
     2161 tggaaaacag cgatattgac cttgttgtag ccggtaccag aaaagcggta accatgattg
     2221 aaggacaggc aaaaaattta tctgaagatg ccatgataca ggcagtagaa tttgcccatg
     2281 caaatatcat taaactgtgc gaaattcagg aagaattgaa aaatacctgc ggcaaaccat
     2341 taatgcagta tataccaaaa ctgagcgata acaagcttga aaaggttatt cgtgagaagt
     2401 actttactgc agttgaaaat tttaaagaat atacggaaaa gaaagcacgt gaagatgcaa
     2461 aagatgctat cataaccgct attaccaatg acctgaaaga acaatttccc gatacaatag
     2521 cacttgttcc tgagattgtt gatgcaatgg atgccgaggt gatacgctca cgtatacttg
     2581 atgaaggttc ccgccctgat ggcagaggac tgaacaatat tcgtcccatt gatattatgg
     2641 ttggcatcct tccacgggct catggttcgg cgctgtttac ccgtggtcaa acacaaagcc
     2701 ttgggatagt tacccttgga actgttgctg agtctcagcg tattgacagc atagatttac
     2761 tggaagaatc atataagcgc ttcatgctgc attactactt cccgccattt agcactggtg
     2821 aagttggcag ggttggcgga gtaggaagac gcgagatagg acatggcatg cttgccgaac
     2881 gcgccattga atatgctatt cccgatgaga aggaatttcc gtatacaata cgcgttgttt
     2941 cagaagtttt agaatcgaac ggttcatcat caatggcaac ggtatgtgcc gggtcacttg
     3001 ctttgtttaa tgccggggtg ccaatgaagg atcaggtagc cgggattgcc atgggattag
     3061 tactggaagg cgagcgatat gcaatcttaa gtgatattca ggggattgag gaccatttag
     3121 gtgatatgga ttttaaggtg gcaggcacgg caacaggtat taccgcattt cagatggata
     3181 tcaagatcga aggcataact accgaaatta tgcgcaaagc gctggagcag gcaaaagaag
     3241 gaaggctcta tatactggaa aagatgaatt cagtcgtttc ccaacctgca caggaactat
     3301 cgccccatgc gccaaaaatt gccgtaatga aaattgagat agaaaagatt ggtgaggtca
     3361 taggaccggg cggaagagta ataaaaaaga ttattgagga aacgggtgcc gagattaaca
     3421 ttgaacagga tggtactatc tttatttcag ccaatgaaca aagcgcaatt gataaggcac
     3481 aacaggcaat caaggggctg atagaagagg ttgaggttgg caccatctat aaaggtgttg
     3541 taaaacgtat taccgacttt ggtgcatttg ttgaagtttt acctggtaag cagggtctgg
     3601 tacatatttc aaaactggat tttaaccgtg ttgaaaaagt caaggacgtg cttaaggaag
     3661 gcgatgaggt aatggttaag gtgataggca ttgatgatca gggcaggatt aatctaagcc
     3721 gtaaagatgc tatgaaaaaa cattagattg tcccatggtt cataaattta cacttcctga
     3781 tgggcttgtt gtcttgttag agccaattga taatgttgta tctatttcgt gcgggttatg
     3841 gatacgggtt ggttcgcggc acgaaaaagc caatgaaatg gggtatgccc atttcattga
     3901 gcatatgctg tttaaaggca ccaaaaaata cagctcaaaa gatattgctc gccttgttga
     3961 tagggttggc ggccagcata atgcagctac caatcgtgag tatacctgct attatatcaa
     4021 tgtggtttct gattatttag aactggcatg cgagatattg tccgatacct actttaattc
     4081 catctttgat gccgaagaga tagaaaaaga aaaaaatgtt gtccttgaag aaatacgcat
     4141 gtacgaagat acccccgatg aacatataca tgatcttttt atggaaaata tgttttatca
     4201 aagtcctctg ggtcatccta tcctgggtgt tgttgatacg gttactacaa ttaatagaga
     4261 ttccattgtt gagttttata aacagcacta tagtgatgat aattgccttt ttgccatctc
     4321 cggcaatttt actgttgata atgcccgtac tattatagaa aaatattttt gcagtaattg
     4381 ttcaccagaa aataatgctc cggctgttat gccacagcca tcgaaacgat tgtaccgcta
     4441 ccatatggac aaggatttag agcagattca tctgtgcatt gggacagatg gattaagtaa
     4501 ggttgatgat gacaggtggg catattatct ctttaatact attctgggcg gcagcatgtc
     4561 atcacgcctt tttcaaaata tacgtgaaaa agagggtttg tgctacagca tatattgttt
     4621 tcattcgtca tttatggaca ccggcatttt tggaatctat tgcgggacat ccccgtcaca
     4681 atataataaa ttattaccgc tgattgccaa tgagtgcaaa caaattgtat cacagggtat
     4741 tacccaggaa gagctttcgg atgccaaaag ctatttaatt ggcaatatgg cattgagcct
     4801 ggagagtact gaagtgcgca tgagccatat agcaaaaaat gagatgatct atgggcgaca
     4861 ttttacgttt caggagatag cgcgtgaaat acaaaccgta accatggatg actttatgcg
     4921 tattgccttc cgcatcttta acaatgcaca gttttcgctt gtgaccattg gaaaactccc
     4981 ccagcatcat atagacacct ttcatcttga tttgtaatgc gatagcaact gtagtttttt
     5041 cttgacaatc gtacattttg ctgtaaaatt tacgattatg agcgaaaatt tacagcatat
     5101 ttaccgatca ttggctgaca tactaccgca agcacacgag cgtaaaatag tgcttattac
     5161 cggtgcgcgt cagaccggca aaactacatt agcaaaaaaa cgatatgctc aattgaagta
     5221 tattaatctt gattcacctg agatgagaga tagtatccgg aaagttgcgg catcagattg
     5281 ggataaggat gtggggatag ctgtccttga tgaagctcag aaagagccca ctatttttga
     5341 aaagataaaa tacagctacg atgaaatgtc attgccgttc tcagttattc tgggatcaag
     5401 ccagatactg ttgttaaaga atatcaggga atcattagca ggcagaatct ggctttatga
     5461 actgtggcct ttaatgatgt ccgaaatggc agcgggtagc agacgggcag aaaagccatt
     5521 gctgggggcc attcttgaag ctgattctat agacacagtg ttgacagcaa agccatcaat
     5581 attgattggc aatgaaaata ctataaagaa aaaggctgag gattatttat tgcaatgggg
     5641 ggggatgcct gcgcttattc accttgaatc ggataatgag aaacatcagt ggctaaagaa
     5701 ttacaattat acctatttag aaagggatat gattgatcta tcacgtctgt atgatcttga
     5761 gccgttccaa aaatttcagc gtctttcagc gtttagattt tcatctttgc ttaattactc
     5821 tgaattagca cgcgatgctg gcattagcac tgatactgca cgcagatatc ttgaatactt
     5881 aaaaatatca tatcaggtgt tattgcttca gccatatagt attaacctta ccagctcgat
     5941 ggtgaagacc ccaaagattt attggattga tatgggcata gtacgacagc taacgggata
     6001 ctacaccaat attccaaccg gacaactata tgaaaacatg gtaattgctg aaatgtataa
     6061 atatattaag acattccata gtaatgcaaa tctttatttt tatagaactc gttcgggatt
     6121 ggaggttgat attgtcatag aaacgccaaa aggattgatg cttgctgaaa ttaaaaacag
     6181 agaagatgtg gtgacaggtg attacactgc tatacgggaa gtggcaaaca agatgggcac
     6241 acggttcatt ggaggaatgg tcatttaccg gggggataaa atatatacat tagctcaatc
     6301 tatatgggct ataccatcac atcgtctttt tacataaacg cttgacaaaa ttgagtcaaa
     6361 atagtactca ttaaccatgt tagatgctat cataacatca aaaacgcgcc ttaagatact
     6421 gctcaagttt tttatcaatc ccagcacaaa aggctacttg cgggagctat cggcacagat
     6481 gaatgagtcc accaatgctg tgcgcgtgga gctggaccgc cttgttgaag caggctattt
     6541 agagcggaac gcaaacggca acaaggtgta ttacaacgcc aacaccatgc atccactgta
     6601 taaggacata cacagcgtgg taaaaaaata tttaggcatt gatgtgctca ttgacagtgt
     6661 gcttgccaag ctggggaagg tgcatgaggc gtatatcgtt ggcgactatt ccaggggtgt
     6721 tgacagcggc atcattgatg ttgtcatcat aggaacggtt gattacacct atttagcgca
     6781 gttgattagc aaagccgaag ctattatcaa acggaagata cgtccgctgg tactatcgcc
//
LOCUS       DAOSOX010000124         6776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11308_length_6776_cov_10.175718, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000124 DAOSOX010000000
VERSION     DAOSOX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11308_length_6776_cov_10.175718"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1366)
                     /locus_tag="PLX22_08950"
     CDS             complement(128..1366)
                     /locus_tag="PLX22_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT20062.1"
                     /translation="MKQKSAPHNYAVFVARQPIFNRHKELYGYEILFRNGFENFYNSL
                     DPDASTSRTLINSFLVIGTDSLTGGKRAFINFTEATLKTSIASFFPKEVLTVEILETV
                     SITPEIISITKKLKSMGYKIALDDFVLSDEYRELLPYADIIKIDFLNTTTFERLQILN
                     EVSRFNTRLLAEKIETYEDFHEALSMGYSYFQGFFFSKPEIIESKDIPIYKLNYWEIL
                     QQIHKEDVDFGKLEDIIKRDVSLSFKLLKFINSAAFGFASEIASIKHALALLGIREFK
                     NWLSFIVLHSIAQDKPQELIVTSLIRAKFAELLASSSQFSTHTSECFLMGLFSLVDAF
                     FDKSKKEILMQLPLTGDIKNAIMDMESPYSGIYKFITAYEKGDWEIVNKLSKQLQFLP
                     EQITQSYRKAIEWANAFFMH"
     gene            complement(1684..2841)
                     /locus_tag="PLX22_08955"
     CDS             complement(1684..2841)
                     /locus_tag="PLX22_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20063.1"
                     /translation="MYDFGLTEEQKLLRQNIRDFAEREIFPVAQELDETETFSVELTR
                     KMGAMGLFGIVVSQNYGGAGMDYLSYVIAVEEIARIDGSQAATVAAANSLGIGPIYYF
                     GSEKQKHEWLPKLCSGKILASFGLTEPGAGSDASASKTNARLENGEWVINGSKIFITN
                     STSSMAGVCTVQAITGKRTDGKKELSCILVPNGTSGFTAVKMTRKMMWRASDTGELFF
                     DDCRVPEENLLGQRGEGFHQMLSTLDNGRLSIAAMGLGGAQGAYELALKYAKQRKQFG
                     QPISTFQAIAFKLAKMATEVEAARNLLYKACRLKDAGMPFSKHAAMAKLYCSEVMGHV
                     VDEAVQIHGGYGLMKEYNVERFYRDYKLLTIGEGTSEIQKIVISRQIGCYE"
     gene            complement(2897..3487)
                     /locus_tag="PLX22_08960"
     CDS             complement(2897..3487)
                     /locus_tag="PLX22_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20064.1"
                     /translation="MSMFGQVYVKNNQYKLKGDFHELTPNIPIRNADEGWRLMGVTNP
                     RDMTYIHAYGGEAIFFESLAKGKLVSSRCDNPKCKYKGSIWMPFRIYCPDCLRPMAVF
                     DMTDLAKKTARIHTFMVCERSGAFNILDKPIKFINIEFDGVVTILMSYLLVGEPKIGM
                     RVVPIFKKVDPTYTILDLSWVPEGTKEEQLPIGFSF"
     gene            complement(3500..4936)
                     /locus_tag="PLX22_08965"
     CDS             complement(3500..4936)
                     /locus_tag="PLX22_08965"
                     /inference="COORDINATES: protein motif:HMM:NF004982.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOT20065.1"
                     /translation="MITFTQQQSKIPKLMRPVYIVTAGVSKFDRAFPDKRTEELCIDA
                     FTMASDMLDMEPAELKGYIHTCYYGHFADHFGDQLLGEAVIHDRLGLDPLGNIGVKTG
                     GATGGSTLWEAVKAVASGYSDCTLAMGWERMDEVPTDEGNFLISCAADKDWESPLGHI
                     YTGYYAVMAQKYWQVFGKEEDSFRRTMAEISVKHHNYARFNPYAQGPMKITIDDVLKS
                     PVVAYPLRALDACLMSVGAACAIICDEETAIKLTQKSKNKPLRIWVAAGSHTLRPADR
                     RDMEIPLLPNETKEQYKDFGKRFPGGERYPGFTGFLAARMAAWYGYRMTGITNPMEDL
                     DVVELHDAFTISDIQTYEDIGLRPYGYGRDYVESGDCYHINPKTGKPGRLPSNLSGGL
                     IGCMHAVGATGIMQVFEIACHLWNRWAEMHGDEKYWKRCKRTKPDDWTNLQVKDAKRA
                     MAISHAGVGSHVTATILMDADSLLKKDA"
     gene            complement(5040..5582)
                     /locus_tag="PLX22_08970"
     CDS             complement(5040..5582)
                     /locus_tag="PLX22_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT20066.1"
                     /translation="MDLGSVLKIKRKEKKMTLKTVASRAGISEGFLSQIENNVNSPSV
                     DTLINICSSIGCDAGEVIQQAQNQKKISIIRKKDLTEIDIPHVGFATLRFFSPESRRT
                     IDSAILMVEPGKSIPARKGIRNSQEVLCVLKGSLELTYQNQVFVLYQGDAAHFITQPE
                     QQHITNNSKMIAVALWVGTI"
     gene            complement(5860..6735)
                     /locus_tag="PLX22_08975"
     CDS             complement(5860..6735)
                     /locus_tag="PLX22_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOT20067.1"
                     /translation="MKLDASFVGTTLKDYTTTISSRAMMNYAAAIDDNNPYYFNDEST
                     EGIIAHPMFAVACTWPISEKICDYINNKNFPLEIIKTQVHYTEHLEFHRPIAPNDTLI
                     IKGKIASIMPHRAGTIITIRFDAYSHNNQPVFTEHIGGMLRGVECTGTVTENSNIPIP
                     GFSEDTSSPCWEKKVYIDRLRPYVYDGCSNIHFPIHTSVAFAKMVGLPDIILQGTATL
                     AYTAREIINHEADGNPHVLKVLSCRFTGMMIPPGNINIALYSKKQVNQNRHLFFEVHD
                     DTGQLLARGNAHLSY"
BASE COUNT         1982 a         1358 c         1200 g         2236 t
ORIGIN      
        1 gaatgcaaat aattaaatga gatccttcac tttgacttca ctcatcatag gcgctattgc
       61 tttacaatga caggatgcat aattattcat tacaagaaaa aaagaattat aacagtatag
      121 caatgtatta atgcatgaaa aatgcgttag cccattctat ggctttgcga tatgattgcg
      181 taatctgctc aggaaggaat tggagctgtt tggaaagttt atttactatt tcccaatcgc
      241 ctttttcata tgcagtgatg aatttataaa tacctgaata agggctttcc atatccatga
      301 ttgcattttt tatatcgccg gttaatggca attgcattaa tatttctttt ttgcttttgt
      361 caaaaaacgc atcaacaagc gaaaacaacc ccattaaaaa acattcagat gtatgtgtgg
      421 aaaattgcga tgatgaagct aatagttcag caaattttgc acgtattaac gacgtaacaa
      481 taagttcctg tggtttatcc tgcgctatgc tatggagtac aataaatgaa agccagtttt
      541 taaattcacg gatgcccagt aatgccagtg catgtttaat agaagctatc tcacttgcaa
      601 atccaaaagc tgccgaattg ataaatttta aaagtttaaa gcttagcgat acatctcgtt
      661 tgatgatatc ttccaattta ccaaaatcaa catcctcttt gtgaatttgt tgtaaaattt
      721 cccaatagtt gagcttataa atgggaatat cttttgattc aattatctca ggtttactga
      781 agaaaaatcc ctgaaaatac gaatatccca tggatagcgc ttcgtgaaaa tcttcatacg
      841 tttcaatttt ttcggctaaa agcctggtat taaaacgcga aacctcatta agtatttgta
      901 agcgttcaaa tgttgttgtg tttaaaaaat caatttttat tatatcggcg tatggcagta
      961 attctctgta ttcatcagaa agtacaaaat catccagtgc gattttataa cccatgctct
     1021 tcaatttttt tgtaatgcta atgatctccg gtgttatgga gactgtttca aggatttcta
     1081 cggttaaaac ttcctttggg aaaaaagatg ctatgctggt ttttaacgtt gcttcggtaa
     1141 aatttataaa agctctttta ccgcctgtca atgaatcggt accaataacc agaaaactat
     1201 tgatgagtgt tctggatgtt gatgcgtctg gatcaagtga attgtaaaaa ttttcaaaac
     1261 cattacgaaa taatatttca tatccatata actctttgtg cctgttaaaa ataggctggc
     1321 gagctacaaa gacagcataa ttatgtggtg ctgatttttg tttcataata aaaatttaat
     1381 tactcattat tgtgcaaaag tatcatatat tcatataatt ggcaacaatt ttattatagt
     1441 aactattggg tggctaacta tattttttgt caataagcaa aaatggtcat tgcgccattt
     1501 ccttctttaa aaagtatata gtattgataa ccgtgatttt gctgcatcat gagatgaaaa
     1561 cactatgggc atctttccca tatttataaa gtgtaaaagg gtatattgta gacatttact
     1621 gtataacaaa actatgcctt gttaccatga ttttttaagg taaattcctt gaaaaatgat
     1681 tatttactca taacacccta tctgacgtga tataacaatc ttttgaattt ctgaggttcc
     1741 ttcacctatt gtcaatagct tgtaatccct gtaaaagcgt tcaacattat attctttcat
     1801 aagcccatat ccgccatgga tttgcactgc ttcatcaact acatgtccca tgacttccga
     1861 acaatataat tttgccattg cagcatgttt tgaaaaaggc atacctgcat cttttaaacg
     1921 gcaggcttta tagagcaaat ttcgcgctgc ctcaacttct gttgccattt ttgctaattt
     1981 aaaggctatt gcctgaaatg tagaaattgg ctgcccaaat tgttttcgct gttttgcata
     2041 tttcagcgcc agttcatatg caccctgtgc acctcctaaa cccattgctg caatggaaag
     2101 cctgccatta tccagggtag aaagcatttg atggaatcct tcaccgcgct gacctaacaa
     2161 attttcttct ggtacccgac agtcgtcaaa aaagagttct ccggtatcag aagcacgcca
     2221 catcatttta cgcgtcattt ttactgcagt aaatccggat gtgccatttg gcacaaggat
     2281 acacgatagt tcttttttgc catcggtacg tttaccggtt atagcctgta ccgtacagac
     2341 acctgccata gaagatgtgg aattggtgat aaagatttta cttccattaa tgacccactc
     2401 gccattttct aaacgggcat ttgtcttgct ggcacttgca tcagatccag cccctggctc
     2461 ggtaagtcca aatgaagcaa gtatctttcc ggaacataat tttggcagcc attcatgctt
     2521 ttgtttttca ctgccaaaat aataaattgg tccaattcct aaagaatttg ctgctgccac
     2581 tgttgctgcc tgtgaaccat caatacgtgc tatttcttca accgctataa cataggatag
     2641 ataatccatt ccggcaccac cataattttg cgaaactaca ataccaaaaa gccccatggc
     2701 gcccatcttc ctggttaatt ctacagaaaa ggtttcggtt tcatccagtt cctgtgctac
     2761 aggaaaaatc tccctttcgg caaaatctct gatattctgg cggagaagtt tttgctcctc
     2821 tgttaagcca aaatcataca tggcaaacct cttttttaaa aatctttatt ttgttttatc
     2881 aaaaaatata tgaatgctaa aatgaaaatc caattggtaa ttgctcctcc tttgtgcctt
     2941 caggcaccca tgaaagatca agtatagtgt atgttggatc aactttttta aaaattggca
     3001 ctacccgcat cccaattttt ggttcaccaa ccaacagata gctcatcaat atagtaacga
     3061 caccatcaaa ttcaatattt ataaatttta tgggtttatc aagaatgtta aatgcgccag
     3121 aacgttcgca taccataaat gtatggatac gagctgtttt ttttgctaaa tcggtcatat
     3181 caaatactgc cattgggcgc agacaatcag gacaatagat tctaaatggc atccatatgc
     3241 ttcctttata tttgcatttt ggattatcac atctggagga taccagtttg ccttttgcaa
     3301 gtgattcaaa aaatatcgct tcgccaccat aggcgtgtat atatgtcatg tcacggggat
     3361 tggttacccc cattaacctc cagccttcat cagcattgcg aattggtata ttaggtgtta
     3421 attcatgaaa gtcacccttc aatttatatt ggttattttt tacgtagacc tgtccaaaca
     3481 tactcattgt attacctcct tatgcatcct tttttaataa gctatcagca tccatcaaaa
     3541 ttgttgctgt cacatgcgaa cctactccgg catgacttat tgccattgct cgttttgcat
     3601 cctttacctg taagttggtc caatcatcag gtttggtgcg tttgcaacgt ttccagtact
     3661 tttcatcgcc atgcatctcg gcccatctgt tccataaatg gcatgcaatt tcaaatacct
     3721 gcatgattcc ggttgcacca acggcatgca tgcagcctat aagccctcct gataaatttg
     3781 atggcagccg acctggtttg ccagtctttg gattaatatg atagcaatcc cccgattcaa
     3841 cataatctct tccataacca tatggtcgta accctatgtc ttcatacgtc tgaatatcgc
     3901 ttatggtaaa agcgtcatgc aattctacaa catcaaggtc ttccattgga ttggtaatgc
     3961 cggtcatacg atagccatac catgctgcca tccgtgctgc taaaaatcct gtgaagccag
     4021 ggtatcgttc gccgccgggg aaacgtttgc caaaatcctt atattgttct ttcgtttcat
     4081 ttggcaatag cggtatttcc atgtctcgcc ggtcagcagg tcgtaaggta tgcgaaccgg
     4141 ctgctaccca tattcgcaat ggtttgtttt ttgatttttg tgtaagtttt attgctgttt
     4201 cttcatcaca tataattgca caggctgctc caacactcat taaacatgca tcaagtgccc
     4261 gcagtggata ggcaaccacc ggtgacttta atacatcatc aatcgttatc ttcattggtc
     4321 cctgtgcata gggattaaat cgtgcataat tgtgatgttt tactgaaatt tctgccattg
     4381 ttcttctgaa agaatcttcc tctttgccaa atacctgcca gtatttttgt gccattaccg
     4441 catagtatcc tgtatatata tgtcctaaag gtgattccca gtctttatct gctgcacagg
     4501 agattaaaaa attgccttca tcggtaggaa cctcgtccat acgttcccac cccattgcca
     4561 gtgtgcagtc agaataacct gaagcaacag ctttaacagc ttcccacaaa gtggagccac
     4621 cggttgcccc accggttttt acaccaatat tacccagtgg atcaagccct aagcggtcat
     4681 ggataaccgc ttcgcctaac agttgatcgc caaaatggtc agcaaaatgt ccgtaatagc
     4741 aggtgtgaat atacccttta agctctgcag gctccatatc cagcatatct gaagccattg
     4801 tgaatgcatc aatacagagc tcctcggtgc gtttgtcagg gaatgccctg tcaaactttg
     4861 atacaccagc tgtaactata taaacagggc gcataagctt tggaattttt gattgttgtt
     4921 gtgtaaatgt aatcataggc taaacctctc tctattattt taatgtatat gcaccataaa
     4981 aatatgcatt gtaccttgct gatagcatat agctatccgt agtcataggc cattataaac
     5041 taaatagtcc ccacccagag agcaaccgca atcatctttg aattattggt aatatgttgc
     5101 tgctcaggtt gagttataaa atgtgcggca tcgccttgat agagcacaaa cacctgattc
     5161 tggtaggtta attcaagcga accttttaaa acacaaagca cctcctggct attgcgtata
     5221 cctttacgag caggaatact ttttcctggt tctaccatca gtattgcact gtctattgtt
     5281 ctacgtgatt cgggtgaaaa aaaacgtaat gttgcaaatc caacgtgtgg tatatctatc
     5341 tctgtgagat cctttttccg tatgatggaa atttttttct gattttgagc ctgctgaatt
     5401 acttcaccag catcacaacc aattgatgaa caaatattta taagcgtatc cacggatgga
     5461 gaatttacat tattttctat ctggcttaaa aaaccttcag agatgccggc gcgtgatgcc
     5521 actgtcttta aggtcatctt tttttctttt cgttttattt ttaacaccga accaagatcc
     5581 attattagtt tctcattagt aaagattcat aaataaaaca actattgtct ttaataatat
     5641 ttactataag taaatattta taatattgtc aataataatt tatcaattta aaaaaattgt
     5701 attttttggc gatagtaacc cttaatcagt gtatgctata gcaagaatgc actgccaata
     5761 ataagtaact atcgcccata gtattgtgaa gattttgtag aaaggtaata gaattttgta
     5821 atgatgatag atttacaatt tttcatggag attgtgcaat cagtatgata gatgagcatt
     5881 tcccctggca agaagctgtc cagtatcgtc atgaacttca aaaaaaagat ggcggttttg
     5941 gtttacctgt ttttttgaat acagggctat attaatgttt cctggcggta tcatcatacc
     6001 tgtaaaacga caggataata cctttaatac atgtggattg ccgtcagctt catgattgat
     6061 aatttcgcgt gcagtatacg ctaacgtagc agttccctgt agaataatat caggcagtcc
     6121 taccatcttt gcaaatgcta ccgatgtgtg tattggaaaa tgaatattag aacaaccatc
     6181 ataaacataa ggacgcagtc tatcaatata aacttttttt tcccagcatg gtgatgatgt
     6241 gtcttcagaa aaaccgggaa ttggaatatt gctgttttcc gtaacggtgc cggtgcactc
     6301 aacaccgcgc aacattccac ctatatgttc ggtaaagact ggttgattgt tgtggctgta
     6361 tgcatcaaat cgtatggtga tgatggtgcc ggcacgatga ggcattattg aagctatttt
     6421 tccttttatg atcaatgtgt cattgggggc tattggacgg tggaattcaa gatgttctgt
     6481 ataatgcacc tgggttttaa ttatctcaag aggaaaattt ttattgttga tgtaatcaca
     6541 aattttttct gatattggcc atgtgcaggc aacagcaaac attgggtggg caatgatccc
     6601 ttcagtgctt tcatcattaa aataataagg attgttatca tcaattgctg ctgcataatt
     6661 catcattgca cgagatgata tcgttgttgt gtaatccttt agcgttgttc cgacaaaaga
     6721 tgcatcaagc ttcatagtaa aaaccttcca attgaattaa atgcattata attatt
//
LOCUS       DAOSOX010000125         6764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11340_length_6764_cov_9.037860, whole genome shotgun sequence.
ACCESSION   DAOSOX010000125 DAOSOX010000000
VERSION     DAOSOX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11340_length_6764_cov_9.037860"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="PLX22_08980"
     CDS             <1..1563
                     /locus_tag="PLX22_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT20068.1"
                     /translation="ILMIITLLTFAAMMEQYRLYEGAQYVNIITYMVISIIAGIFVMY
                     INSDSVMRVLVEMGVVTKNIASGQNAWFKVMSHEEEFAEIEYSILEMATQIEEHRKNL
                     ELKVEQRTEELREALASLKEKDDMIQKQLEIASNIQRSILPGRIDDWNELKFSVRYIA
                     MEKIGGDFYDVYQIKGDKLGVLVADVSGHGIPAALITSMAKISFSSATQQYDSPKRVF
                     QEVNQNILEHIKTQDYMTAFFVVIDDDYNIVYANASHQKAILVHSDEGRVEFLDTNGL
                     FIGAIEEARETYEEKVTKLRYGDRLLLYTDGIPEAANVDREEYSIERLQDAALKNRHL
                     PLEDFTSFIIEDVQRFKGKAPVEDDITLLVIELARDEAVDIIKQSKKLIDEHKYYEAI
                     DYLERSLTLYPNNRKLIYNLAKNYFRVNNFGKASELIEQYLEMDKRNKYAYYVGGAAY
                     YQMMNYEKAIQLLERAQSIDPNFTNALFALGMAYKKIGMSQDALQIFERVANIDPDNK
                     MALFEINEIRKK"
     gene            1624..>3502
                     /locus_tag="PLX22_08985"
     CDS             1624..>3502
                     /locus_tag="PLX22_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT20069.1"
                     /translation="MLLKKRNKKSSKKNKPIRKVYFGLRLRLILVLSVVLLIVLLIFT
                     AFMIYNQTIIMSQEKNEKATILAQNLSNIFEWYLDKSKYETIEDSKQKLKLIKEEITK
                     FKNNNQDILYIFLYDYYNTIAYKSTQIDITKEPYYKVVKSYLTDRQQKLITHDYTAGK
                     DISKNNKFYKNKQYKVVLCPVIAKTGILMQINEDFDTILLKSHTQSFTNDEKKKFYTK
                     LWVKYHDLLPDKFDPKNFDKKNENLIDCNLDIIFLQLYKNIFSIKKNVVEKKDLWLLN
                     EKWILHNYTIISRGILNKDIALIKKYDDIIIDNLKVLRDYGEKFKHLGTLIVVYDLNQ
                     IQSDIYKSLSNGYITALSIYIISIMVIFWITGIMLKNIKKLEKWALDVGKGNFSLPID
                     IKNNDEIGRLSDVFQYMINEINSKYHLEKFVSRSTRHLIGMAGFEKIDVGFFDRRNFA
                     FLFSDIRGFTSFSENNDPETVMSVLNLYFDKQAKIIQKYKGDIDDFVGDQIMAHFGGT
                     KKADTAITVAIGIMKEIDLLNKERKSMNLPIFEIGIGIHVGDVVIGKIGAEFRMDLAC
                     IGDVVNTTSRLCQNAQAGEIIVSKSIISEITKTFKYKKLEPVKLKGKKDPLEVYSVKW
                     ES"
     assembly_gap    3503..3602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3603..3808)
                     /locus_tag="PLX22_08990"
     CDS             complement(<3603..3808)
                     /locus_tag="PLX22_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20070.1"
                     /translation="MFTIVVISEIHGQIKSKQRDENTVEYYNIGNKKEKGNNYQQTNI
                     LKNPYDSLIVTIANKENIDPHLIR"
     gene            complement(3852..5297)
                     /locus_tag="PLX22_08995"
     CDS             complement(3852..5297)
                     /locus_tag="PLX22_08995"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT20071.1"
                     /translation="MNITFITSEAYPFAKTGGLADYSYSLPKALAKLGHTVNLFLPRY
                     YCVDKIKYNCSLIDMPLAVPHDDGIRWAGIYKSNYLDTINVYFIEHDVFFGRDGLYDY
                     HGIPYGDNAERFIYFNRATLEALKQLAIVTDIIHCNDWQTALIPLFLKTHYAHESIFK
                     NTKSVLTMHNAGYQGVFPKDVLYYMQLSDEYFVPERLAFYDKINFLKAGILYADCLTT
                     VSKRYAQEIQYPDYGYDLAPIIKQNSSKLFGILNGVDYEQWNPEYDKFIPCNFSINDV
                     SGKKKCKYALQDYFSLNKSDIPLLGCISRLTYQKGIDILIDALDWLLFDEPEVQFVLL
                     GNGEQWLIDRFENLRKLYPDKVGVWWGYNEKLSHCIEAGCDFYVMPSRYEPSGLNQMY
                     SMAYGTIPIVRATGGLDDSVIEFNTTELTGNGFKYKHNDIAELYSTIRYALKIYNNSN
                     LMQQLIYNAMSFKRSWDDAAKEYEALYKNIK"
     gene            complement(5299..>6764)
                     /gene="gpmI"
                     /locus_tag="PLX22_09000"
     CDS             complement(5299..>6764)
                     /gene="gpmI"
                     /locus_tag="PLX22_09000"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOT20072.1"
                     /translation="PSDADMGNSEVGHNALGAGRIFDQGAKLVDKSIESKQIFTTDVW
                     KKLIDKPVKSKSTLHFIGLLSDGNVHSHINHLFKLIEQAAHEGVKKIRVHILLDGRDV
                     PETSALEYVSSLENFLASFNARGFDFCIASGGGRMVTTMDRYEADWNIVKRGWDAHVL
                     GKGRAFTSATEAIETFRKEQPDITDQYLPAFVITKNGKPAGTIQDGDSVILFNFRGDR
                     AIEISRAFEDKNFNKFDRERYPDVVFAGMMEYDGDLHIPKNYLVFPPSIDKTVSEYLV
                     HNGCKQFAISETQKFGHVTYFWNGNRSGMFDPQYEVYQEIPSDRVQFDQRPWMKAAEI
                     TDAVIEALNSNKYDFIRLNFPNGDMVGHTGNLEAAIIAAEVVDLCIGRLLKAINDTQA
                     LAIITADHGNLDEMFEKDTKTGAIIIDKKTGKPQPKTSHTLNPVPFIIYDPHFKGEYC
                     LTPQTNQGLANVAATICTLLGYHPPEEYYPSLITLAQ"
BASE COUNT         2365 a         1023 c         1145 g         2131 t
ORIGIN      
        1 atactaatga ttattacact gttaacattt gctgcaatga tggagcaata tcgtttatat
       61 gaaggagcac agtatgttaa tatcattaca tacatggtta tttcaataat agcaggtatt
      121 tttgtaatgt atattaattc tgactcggtt atgcgggtac ttgtagaaat gggtgttgtt
      181 acaaaaaata ttgcttcagg gcaaaacgca tggtttaagg taatgtcaca tgaagaagaa
      241 tttgctgaga ttgaatattc catattagaa atggcaacgc aaatcgaaga acatagaaaa
      301 aatctggaat taaaagttga acagcgtact gaagaattgc gcgaagcatt agcaagctta
      361 aaagaaaaag acgatatgat acaaaagcag cttgaaattg caagtaatat acagagaagt
      421 attttacctg gaaggataga tgactggaat gagcttaaat tttcggttcg atatattgca
      481 atggaaaaaa ttggagggga cttttatgat gtataccaga ttaaaggaga taaattaggt
      541 gttttagtgg cagatgtatc cggacacggc ataccagcag ctctcattac atcaatggca
      601 aaaatctcat tcagttcagc aacacaacaa tatgattcac ctaaacgtgt tttccaggaa
      661 gttaatcaga atattcttga acatataaaa acacaggatt atatgacagc attttttgtt
      721 gttattgatg atgattacaa tattgtttat gcaaatgcaa gccaccagaa agcaatttta
      781 gtacattcgg atgagggtag agttgaattt ctggatacca atggtttatt tattggtgct
      841 attgaagaag caagagaaac atatgaagaa aaagttacaa aattgagata cggtgacaga
      901 ttgcttttat atactgacgg catacctgaa gctgcaaatg ttgaccgtga agaatattca
      961 attgaaagat tgcaggatgc tgctttgaaa aaccggcatc ttccattaga agattttacc
     1021 agctttatta ttgaagatgt gcagcgattt aagggaaagg ctcctgttga agatgatatt
     1081 acgttacttg ttattgaact ggcaagggat gaagctgttg acattataaa acaatcaaag
     1141 aagttgatag atgaacacaa atactatgaa gcaattgatt atcttgaaag gagtttaacg
     1201 ctatatccaa ataatcgtaa gcttatatac aatttagcaa aaaattattt ccgtgttaat
     1261 aattttggta aagcatcaga acttattgaa caatatttag aaatggataa acgtaataag
     1321 tatgcttact atgttggggg cgcagcatat tatcaaatga tgaactatga aaaggcaatt
     1381 caattgctgg aaagagcaca gagcattgat cctaatttca ccaacgcttt atttgcttta
     1441 ggtatggcat acaaaaaaat aggaatgtct caggatgcat tacagatatt tgagcgtgta
     1501 gctaacatag atcctgataa taagatggca ttatttgaaa taaatgaaat aagaaaaaaa
     1561 tgataaaagg caaaacattt ttatcatttc aatattatta tattgaataa acgaatatct
     1621 actatgcttt taaaaaaaag aaataaaaaa tcttcaaaaa aaaacaaacc aatcagaaaa
     1681 gtatattttg gtcttagatt gcgtttaata ttagtactaa gtgttgtttt gctcatagta
     1741 ttattaatat ttactgcatt tatgatttat aatcaaacta ttattatgag ccaggaaaaa
     1801 aatgaaaagg ctacaatttt agcgcaaaac ctgagtaata tatttgaatg gtatttagat
     1861 aaaagcaaat atgaaacaat agaggattca aaacaaaagt taaaattaat aaaagaagaa
     1921 ataacaaaat ttaaaaacaa caatcaagat atattatata tatttcttta tgattattat
     1981 aatacaatag catacaaatc aacgcaaatt gacattacta aagaaccata ttataaagtt
     2041 gtaaaaagtt atttaacaga tcgtcagcaa aaattaatta cgcatgacta tactgctgga
     2101 aaggatatat caaaaaataa taaattttat aaaaataaac agtataaagt tgttctatgc
     2161 ccggtaatag caaaaactgg catattgatg caaattaatg aagatttcga tactatactt
     2221 ttaaaaagtc atactcaaag ttttacaaat gatgaaaaaa agaaatttta tactaaattg
     2281 tgggttaaat atcatgattt attgcctgac aaatttgacc caaaaaattt tgataaaaaa
     2341 aatgaaaatt taatagattg caatcttgat ataattttcc tccagctata taaaaatatt
     2401 ttttctatca aaaaaaatgt agtagagaaa aaagatttat ggttactcaa tgaaaaatgg
     2461 atattacaca actatacgat tatttccaga ggcattttaa ataaagacat tgctcttatt
     2521 aaaaaatatg atgacattat aatagataat ttaaaggttt tacgagatta tggagaaaaa
     2581 tttaagcatt taggtacttt gattgttgta tatgatttga atcaaatcca gtcggatatc
     2641 tataaaagcc tttccaatgg atatataaca gcattatcaa tatatattat aagtataatg
     2701 gttattttct ggattacagg gataatgtta aaaaatatta aaaagttaga aaaatgggct
     2761 ttagatgtag gtaagggaaa tttcagtttg cctattgata ttaaaaataa tgatgaaatt
     2821 ggaagattat cagatgtttt ccagtatatg ataaatgaaa taaattcaaa atatcatctg
     2881 gaaaagtttg tttcaagatc aactagacac ctgattggaa tggcaggatt tgaaaaaatt
     2941 gatgtaggtt tttttgatag aagaaatttt gcgtttttgt ttagtgatat cagagggttc
     3001 acaagttttt ctgaaaataa tgatcctgaa actgttatga gtgttttgaa tttatatttt
     3061 gataaacagg caaagattat acaaaaatat aaaggggata tagatgattt tgtaggtgat
     3121 cagataatgg cgcattttgg cggtaccaaa aaagctgata cagcaattac agttgcaatt
     3181 ggaattatga aagaaattga tttactaaat aaagaaagaa aatcaatgaa tttacctatt
     3241 tttgaaatag gtataggtat acacgtaggt gatgtcgtta taggtaaaat tggtgcagaa
     3301 tttagaatgg atcttgcatg cataggtgat gtagtaaata caacatcacg attatgccaa
     3361 aatgcacagg ctggcgaaat aattgtatca aaaagtatta taagtgaaat aacaaaaaca
     3421 tttaaatata aaaagttgga acccgttaaa cttaaaggta aaaaagatcc attagaagta
     3481 tattctgtta aatgggaatc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nncatctaat taaatgagga tcaatatttt ccttattggc gatagttact attaatgaat
     3661 catatggatt tttaagaata tttgtttgct gataattatt tcccttttcc tttttattcc
     3721 cgatgttata gtattctaca gtattttcat ctctctgttt ggatttaatc tgcccatgaa
     3781 tctctgatat gacaacaata gtaaacatta atatcattgc aattatcaat gtatgatgag
     3841 caaaatgtat attacttaat atttttatac aatgcttcgt attcttttgc tgcatcatcc
     3901 cagctccgtt taaaagacat cgcattatat attaattgct gcattaaatt gctattgtta
     3961 taaatcttta atgcataccg aatggttgaa tataattcag caatatcgtt atgtttgtat
     4021 ttaaatccat ttcccgtgag ctctgttgta ttaaattcaa tgactgaatc atcaagccca
     4081 cccgttgccc ttacaatggg aatggtaccg tacgccatgc tatacatctg gtttaaacct
     4141 gaaggctcat agcgtgatgg cataacataa aaatcgcatc cagcttcaat acaatgagat
     4201 aatttttcat tgtaccccca ccaaacaccg accttatcag gataaagctt ccgaaggttt
     4261 tcaaatctgt caataagcca ttgctcccca ttccctaata gtacaaattg cacctctggt
     4321 tcatcaaaca aaagccaatc caatgcatca atcagtatat caataccttt ttgatatgtc
     4381 aacctgctaa tacaccctaa caatggaata tcacttttgt ttaatgaaaa gtaatcctgc
     4441 aaagcatatt tgcacttttt tttgcctgat acatcgttga ttgaaaagtt acagggtata
     4501 aatttatcat actcaggatt ccattgctca taatcaacac cgttgagaat gccaaaaagt
     4561 tttgagctgt tttgtttaat aattggtgcc agatcataac catagtcagg gtattgtatc
     4621 tcctgagcat agcgtttact gactgttgtt aaacaatctg catatagaat gccagcttta
     4681 agaaagttta ttttatcata aaatgccaat cgttctggaa caaaatattc gtcagatagt
     4741 tgcatataat ataatacatc ttttgggaaa acaccctgat atccagcgtt atgcatggtt
     4801 aaaacgcttt tagtattttt aaatatagat tcatgagcat agtgggtttt taaaaataga
     4861 ggtatcaatg ctgtctgcca gtcattgcaa tgaatgatat cggtaactat cgccaattgc
     4921 tttaacgcct caagggtagc acggttaaaa tatataaacc gctcggcatt atcaccgtac
     4981 gggataccgt gataatcata taatccatca cgaccaaaaa aaacatcatg ctcaatgaag
     5041 taaacattga tagtatcaag ataattagat ttatatatac cagcccagcg aataccatcg
     5101 tcatgaggta ctgctaacgg catatcaatg agtgagcagt tgtattttat tttatcgaca
     5161 caataatagc gtggtaaaaa aaggttaacc gtatggccca gttttgccag cgcttttggc
     5221 aatgagtatg aataatcagc aagaccacct gtctttgcaa atggataggc ttcacttgtt
     5281 ataaatgtta tattcatatc attgtgcaag ggtaatcaat gagggataat attcttcagg
     5341 tggatgatat ccaagcaatg tgcatatagt agcggctaca tttgcaagtc cctgattggt
     5401 ttgcggcgta aggcaatatt cgcccttaaa atgggggtca tagattataa acgggacggg
     5461 atttaacgtg tgtgaagttt ttggttgcgg cttcccggtc ttcttatcaa tgataatggc
     5521 tcctgttttt gtatcttttt caaacatttc atctaaattg ccatgatccg cagttattat
     5581 agctaatgcc tgtgtatcat taattgcttt taataaacgt ccaatgcaca gatcaacaac
     5641 ctctgcggca atgattgcag cctctaaatt accagtatgt cctaccatat cgccattggg
     5701 aaaatttaag cgaataaaat cgtatttgtt gctatttagt gcttcaataa ctgcatcagt
     5761 aatttcagca gctttcatcc atggacgctg atcaaattgc accctgtcag aggggatttc
     5821 ttgatacact tcgtactgtg ggtcaaacat cccgctacgg ttaccattcc agaaataggt
     5881 aacatggcca aatttttgtg tttctgaaat ggcaaattgc ttacagccat tatgaacaag
     5941 atattcactg acggttttat caattgacgg aggaaaaaca agataatttt ttggtatatg
     6001 taaatctcca tcatattcca tcatccctgc aaacacaaca tcgggatatc gttcacgatc
     6061 aaatttatta aaatttttat cctcaaaagc ccgtgaaatc tcaattgccc ggtcacctct
     6121 aaaattaaat agtataaccg aatcgccatc ctggatagtt ccagcaggct tgccgttttt
     6181 tgtaatgaca aaagcaggta agtactgatc ggtaatatcg ggctgttctt ttctaaatgt
     6241 ttcaatagct tctgttgcag atgtaaatgc tcgccctttg ccaagaacat gagcatccca
     6301 cccacgcttt acaatattcc agtctgcttc atatctatcc atggtggtta ccatcctgcc
     6361 tccaccagag gcaatgcaga aatcaaatcc tcgtgcatta aatgatgcca aaaaattttc
     6421 aagactgcta acatattcca gtgcagaagt ttcagggaca tcacggccat caagaagtat
     6481 atgaacgcga atctttttta ctccttcatg agctgcctgt tcaatgagtt taaacagatg
     6541 gtttatatga gaatggacat taccgtcaga aagtaatcct ataaaatgaa gtgttgattt
     6601 agattttact ggcttatcaa taagtttttt ccatacatca gttgtaaaaa tttgttttga
     6661 ctctatggat ttatcaacca gttttgcacc ctgatcaaaa atccttcctg ctcccaatgc
     6721 attgtgccca acctctgagt ttcccatatc agcatccgat ggta
//
LOCUS       DAOSOX010000126         6691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11499_length_6691_cov_7.694093, whole genome shotgun sequence.
ACCESSION   DAOSOX010000126 DAOSOX010000000
VERSION     DAOSOX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11499_length_6691_cov_7.694093"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLX22_09005"
     CDS             complement(<1..759)
                     /locus_tag="PLX22_09005"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOT20073.1"
                     /translation="MYTEKNISALHNIIRDLTEIDSRLNEYIDTTMWISDPHGAGDRF
                     VSILKGRFGLVWRICYEALPKTFSKEKIYYLGRIIRKERYFEDDVYRLDRQDIISSLI
                     RIIQYRVQNVRDFDEIRININKDLKHVLENLILNYPVPDIIYENELIADKIISSLCKI
                     VKQVILGHLIVLGDVFDRGDEPDKIIRILNQKDIKRYLTFIWGNHDILWMGASAGNQS
                     LIAEALRITTRYDNHAFLERIGINIDKLKNFALST"
     gene            complement(783..1271)
                     /locus_tag="PLX22_09010"
     CDS             complement(783..1271)
                     /locus_tag="PLX22_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOT20074.1"
                     /translation="MSLCKVDIVTIVLDEESNTPIVVLQDNETGNILPIMIAPLEASL
                     IAIELEGKKPIRPLTHDLIIAMLHTFHYSLESVVIDDLKDNIYFAKLNLVAPDSSEMI
                     IDCRPSDAMAIALRAKAPIYVKKKVFAIAQGLDNLSQIDKETMKEVLEDIDIDDVGGK
                     IM"
     gene            complement(1337..2224)
                     /locus_tag="PLX22_09015"
     CDS             complement(1337..2224)
                     /locus_tag="PLX22_09015"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT20075.1"
                     /translation="MELSEYAGQSTRINDFYIHVSDGIQLYCIDFIPKHDNVSKPIIF
                     FVAGWLSHISGWKDVLRSLTDEYHVIYIETREKISSKIPDGKSCTDFSFNVMRMVLDL
                     HEVIQQIIPKKRTFVMAGSSLGSSVILEYLAMRTREPECALLIGPIPVFRFPPILGDI
                     VPNLPPSWYNVLKKLVIWYLKTFRLNTRKEKEQLIKYTNTINAADPYKLKPNALSLKN
                     YSLWDKLAFIETPCYIFGAKTDTLHATEDIKRLLQELNRADYIECESNKETHSAIVAL
                     YFINILKNKLYKKIKAKKP"
     gene            complement(2369..3391)
                     /locus_tag="PLX22_09020"
     CDS             complement(2369..3391)
                     /locus_tag="PLX22_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT20076.1"
                     /translation="MKKIYSPMVIVAMFGAQLLLSCSHPQRYSLSQMAMGTIVTITVD
                     APSHEKAHNAIQAAFDKIKQIEALMSLYKQDSDVSKINANSKTHVSKDTFTVIEQSLE
                     LSHKTNGLFDITFASIGKLWNIQSPNFIPPSPQTINTYKPCIGYQRIHLHPENLEVIK
                     EKPCIQIGLGGIAKGYAIDHAIVTLKNMGIKEAIVEAGGDLKVIGNNYGHGYTIAIKH
                     PRKEGICGTVLLFNGQSITTSGDYERFVIYKGKRYHHILNPKTGYPATSDVISVSVIA
                     SNATVCDGYATALFIMGSEQAMSFLLHNASLQLQCIIIDSTMKIYVSKSLVPCTSFKG
                     YTVVPF"
     gene            3994..5397
                     /locus_tag="PLX22_09025"
     CDS             3994..5397
                     /locus_tag="PLX22_09025"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT20077.1"
                     /translation="MKMKFHGITVCVIILCAIVTVDAKNTIKKTQQGTSIADLNTFNE
                     QLVTITNKAIPTVVSISAEKKMGSPRDARKMPMPFPMPKEAKAVGSGVIVDKRGYIVT
                     NNHVVKNTKSITVTLFDKRTFPCELIGSDPATDIAVIKIQGDVPKDIPVAEMADSDTL
                     KVGEIAIAIGNPFGFGHTVTMGIISATGREGFGLADYEYFIQTDAAINPGNSGGALIN
                     VTGKLVGINTAIFSRNGGYMGIGFAIPSNMVKQVVDELIQKGKVTRGWLGVYIQNITP
                     EIAKNFKYDGKQGVVVADVMNNSPAKNANLEIGDIIVSVNGTTVSDINQLRRNVAALK
                     PGSTAKVKIFRAGKEMDVDVIIAELPQQQAMEEKAVELDIIGLRVGDITEENAYKFRI
                     TDTTGVIVMEVSEDGPAYQAGMMVGDIIKEIENTPVENVSRYNELLAHYQKKETLLLL
                     VKRGGIHKFVVIKKSEK"
     gene            complement(5421..6395)
                     /gene="trpS"
                     /locus_tag="PLX22_09030"
     CDS             complement(5421..6395)
                     /gene="trpS"
                     /locus_tag="PLX22_09030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT20078.1"
                     /translation="MSRILSGIQPSGTLHIGNYFAMMKPMIELQYNNELFCFIVNYHA
                     LTSIHDPRLLAHNTLNAAMDFIALGLDPDKAHFWVQADIPEVTELSWILSCHTGMGLL
                     ERSHSYKDKIAKGIIPNAGLFTYPVLMAADILLFNAELVPVGKDQKQHLEITRDIAQH
                     FNQMYGEIFVIPEAMIPEDIAVIPGIDGQKMSKSYNNTINIFEDEASLKKKVMRIVTD
                     SQPVEAPKNPDSCNLFALYRLFAEPEKTQILKDRYLKGGIGYGEVKKELFAMIWEYFA
                     PFRAKRKALENNKHDIASILKKGAEKTRPLAIETIQRVKEATGLKYLK"
BASE COUNT         2094 a         1139 c         1235 g         2223 t
ORIGIN      
        1 tgtagatagt gcaaaatttt ttaatttgtc aatatttata ccaatacgtt caagaaaagc
       61 atgattgtca taacgagtag tgatacgtaa agcttcagca ataagtgatt gatttccggc
      121 agatgctccc atccataata tatcatgatt gccccagatg aatgtaagat atcgctttat
      181 gtctttttga ttaagaattc ttattatttt atcaggttca tctcccctgt caaatacatc
      241 gccaagtaca attaaatgcc ctaatataac ctgttttaca attttacaca gtgaactgat
      301 gattttatct gctattaatt cattttcata aattatatct ggaacaggat agtttaatat
      361 aaggttctca agaacatgtt taaggtcttt atttatatta atacggattt catcaaaatc
      421 acgaacattt tgtacgcggt attggatgat tcttataagt gaggagatta tatcctgacg
      481 gtctaaccta taaacatcat cttcaaaata acgttctttg cgaataatcc tgcctaaata
      541 gtatatcttt tcttttgaaa atgttttggg taatgcttca taacatatgc gccataccag
      601 gccaaagcgt ccttttaata ttgacacaaa tctatctccc gcaccgtggg ggtcgcttat
      661 ccacatggtt gtatcaatat attcgttcag gcgggagtct atttcagtaa ggtcgcggat
      721 aatattatgc aatgctgata tgtttttttc ggtgtacata gatcctccgc taataaaaaa
      781 atttacatga ttttaccgcc aacatcgtct atgtcaatat cttcaaggac ttctttcatt
      841 gtttctttat ctatctgtga aagattatct aatccctgag ctattgcaaa tactttcttt
      901 ttgacataaa ttggtgcttt tgctcgtaat gcaattgcca tagcatcact ggggcgacaa
      961 tcaatgatca tttcagaact atctggagca actaaattaa gctttgcaaa ataaatgtta
     1021 tcttttaaat catcaattac tactgattca agagaataat gaaatgtgtg aagcattgct
     1081 ataattaagt cgtgtgttaa aggacgtatt ggttttttgc cttcaagctc aatggcaata
     1141 aggcttgctt ctaaaggagc aatcatgatg ggcagaatgt ttcccgtttc attgtcttgc
     1201 agaacaacta taggtgtatt gctttcttca tccagaacaa tagtaactat gtcaactttg
     1261 cataatgaca tctcaattcc ttttttgcgt tatttgtatt caatataaaa tgaatatata
     1321 aaaaaatcaa gtaaagttaa ggctttttgg cttttatttt tttatacagc ttgtttttaa
     1381 ggatatttat gaaatataga gcaacaattg cactgtgggt ttctttattg ctctcacatt
     1441 ctatgtaatc agcacgattt agttcctgca gcaatcgttt tatgtcttca gtagcatgca
     1501 atgtgtctgt ttttgcacca aatatataac atggcgtttc aataaaagca agtttatccc
     1561 atagactata attttttaat gatagtgcat tgggtttgag cttataagga tcagcagcat
     1621 tgatggtatt ggtatatttg ataagctgtt ctttttcttt tctggtattt aatctgaaag
     1681 tttttaaata ccatataact aattttttta acacgttata ccatgatggg ggcaaattgg
     1741 gaacgatatc tcctaatatg ggaggaaatc tgaatacagg gattggccca atgagaagtg
     1801 cacattcagg ttcacgtgtt cgcatggcta aatattctaa aattacagaa gaacccagtg
     1861 aactgcctgc cataacgaat gttctttttt taggtattat ctgttgtatt acttcatgaa
     1921 gatcaagaac cattctcatg acgttgaaag aaaaatctgt gcatgatttg ccatcaggta
     1981 ttttagaaga aattttttcc ctggtttcaa tatatattac atggtattca tcggtcaatg
     2041 aacgtaaaac atctttccag ccggatatat gggaaagcca tcctgcaaca aaaaaaatta
     2101 ttggttttga tacattgtca tgttttggaa taaaatctat gcaatagagt tgtattccat
     2161 cagatacatg aatatagaag tcattgattc ttgttgattg cccggcatat tcagaaagtt
     2221 ccatagtcat cctcattaaa aagttttacc atttccacac tgttaatcaa tcataaacaa
     2281 ttaagaattg caactatttt gtcattggga aaaataaatt ttttagcaac ttgatttatg
     2341 gtcagtaact atcgccaaaa ataataaact aaaaaggaac cacagtatat cctttgaacg
     2401 aagtgcaggg caccaaactt tttgatacat atattttcat tgttgagtca ataataatac
     2461 attggagttg taacgatgca ttgtgcaaca aaaacgacat tgcttgctct gaccccatta
     2521 taaataaagc agttgcatat ccatcgcata cagtagcatt gctggcaatc accgaaacag
     2581 agatgacatc acttgtggca ggataccctg ttttaggatt gagtatatgg tggtagcgtt
     2641 tacccttgta aataacaaag cgttcgtagt cgccgcttgt agtaatggac tgtccattga
     2701 acagcagtac ggttccgcaa attccttctt tacgcgggtg ctttattgca atggtgtagc
     2761 catgaccgta attgttacca ataaccttaa gatcaccacc agcttcaaca atagcttctt
     2821 tgatacccat atttttcaat gttactattg catggtctat tgcatatcct tttgctatgc
     2881 cgccaagacc aatctgtata cagggttttt ctttgatgac ctcaagattt tccggatgga
     2941 gatgaatacg ttgatatcct atacacggct tataggtatt gatggtttgt ggtgatggag
     3001 gaatgaagtt tggcgattgt atgttccata atttgcctat tgatgcaaag gttatgtcaa
     3061 aaagcccatt tgttttgtgc gatagttcca gggattgttc aatgacggta aaggtgtctt
     3121 tgcttacatg agttttgcta tttgcattta tttttgacac atcactgtcc tgcttgtaaa
     3181 ggctcatcaa cgcttcaatc tgcttgattt tgtcaaatgc tgcttgtatt gcattgtgtg
     3241 ctttttcatg cgatggagcg tcaacagtga tggttactat tgtacccata gccatttgtg
     3301 acagactata gcgttgtgga tgtgagcatg acagtaataa ctgcgcacca aacattgcta
     3361 ctataaccat cggtgaataa atctttttca ttcgctaatc catgatatta tttacaataa
     3421 attttctctg agattcgttg aaaataataa tatacgatag tatcatttta aagaaaaaac
     3481 gcaactgaaa aataatcttc actcattatc attctgatgg cacacagtgt caaagaaaat
     3541 ctcctcacac taatgagatt ctttgctaac gctcagaata acactcaaca cattgtcatt
     3601 tcggacctga ctcagaatcc aggaatgtaa cgttaaatac tgaaacacga tttcaggata
     3661 actatcgcca gatagttttg tctttttggc gaggtcataa gccacccatc gtgatcttct
     3721 ttatacacta tagcattata tcgatataga aaaaaataaa acactggtgg tattaatggt
     3781 gctcatcata acgattaatt aaattaaaac ctcaaagcta aatgacttga caacaatgat
     3841 agtatgatta taagaaataa aggtattaca atagttattg ctattaatac tgcaccgaac
     3901 aatggtataa cgttatcatg cagtgttaat aaaattgaat acatatactt ttaaatgcaa
     3961 taaaaatatt tttttcatta caaggaatgc attatgaaaa tgaaatttca tggtataaca
     4021 gtttgtgtca ttattttgtg tgcgatagtt actgttgatg caaaaaacac aatcaaaaaa
     4081 acacagcaag gaacatcgat tgctgattta aatacattca atgagcaatt ggtgaccata
     4141 accaataaag caattcctac tgttgtgagt atatctgccg agaaaaaaat gggttcaccg
     4201 cgtgatgcca gaaagatgcc aatgccgttt cctatgccaa aagaggctaa agctgttggt
     4261 tcaggtgtta ttgttgataa acgaggatat atagtaacca ataatcatgt tgtaaaaaac
     4321 acaaaatcaa taaccgttac tttatttgat aagcgtacat tcccatgtga gctcataggc
     4381 tctgaccctg ctactgacat tgcagtaata aaaatacagg gagatgttcc caaagacatt
     4441 cctgttgctg aaatggccga ttcagacaca ttaaaggttg gagagattgc catagctata
     4501 ggaaaccctt ttggatttgg gcatacggta acgatgggaa ttatcagcgc aacaggcaga
     4561 gaaggttttg ggcttgctga ttatgaatat tttattcaaa ctgatgcggc aataaatccg
     4621 ggaaattctg gcggggccct tattaatgtt acaggaaaac tcgtaggtat aaatacagca
     4681 atatttagcc gaaatggcgg ttatatgggt atagggtttg ccataccttc aaacatggta
     4741 aagcaggttg tagacgaact gatacaaaaa ggtaaggtaa cgcgaggctg gttgggtgtg
     4801 tatattcaaa acattactcc tgaaattgct aaaaatttta aatatgacgg taagcaaggg
     4861 gttgtcgttg ccgatgttat gaacaattca cctgcgaaaa acgctaatct tgaaattggc
     4921 gatattattg tttctgtcaa tggcacaaca gtttcggata taaatcaatt acgccgtaat
     4981 gttgccgctt taaagcctgg ctctactgca aaagttaaaa tctttagagc aggcaaagag
     5041 atggatgttg atgtcatcat tgcagaactc cctcaacagc aagctatgga agaaaaagca
     5101 gtagaacttg acataattgg gctgcgtgtt ggcgatataa ccgaggaaaa tgcatataaa
     5161 ttcaggataa ccgatacaac aggtgttata gtcatggagg ttagtgaaga cgggcctgca
     5221 tatcaggcgg ggatgatggt tggtgacatt atcaaagaga ttgaaaacac gccggtagaa
     5281 aatgtatcac gctataatga gctattagca cactatcaaa agaaagagac gctgctttta
     5341 ctggtaaagc gtggagggat ccataaattt gtagttataa aaaagagtga aaaataatag
     5401 catataaggg attaatattt ttattttaaa tattttaatc ctgtggcttc tttaacccgt
     5461 tgaatggttt ctatagccag tggccgggtt ttttctgccc ctttttttag tatggatgct
     5521 atgtcatgtt tgttattttc aagcgctttt cgtttagctc taaatggtgc aaaatattcc
     5581 catatcattg caaaaagctc tttttttact tcaccatatc ctatgccacc ctttaagtat
     5641 cggtctttaa gtatttgtgt tttttcaggt tcagcaaata gcctgtacaa agcaaataga
     5701 ttacagctat caggattttt aggtgcttct actggctgcg agtcggtaac tattcgcatt
     5761 accttctttt taaggctggc ttcatcttca aatatattga tggtattgtt gtagcttttt
     5821 gacatcttct gtccatcaat acccggtatt acagcaatat cctcaggaat cattgcttcg
     5881 ggtataacaa agatttcacc atacatctga ttaaagtgct gtgcaatatc tcgtgttatc
     5941 tcaaggtgtt gtttttgatc cttgccaacc gggactaact ctgcattaaa taaaagaata
     6001 tcagcagcca tcagtacagg ataggtaaaa agtccggcat tggggataat cccttttgca
     6061 atcttatcct tatagctatg acttcgttca agaagcccca tgccggtgtg gcatgatagt
     6121 atccatgata attcggtaac ctcagggata tcagcctgca cccaaaaatg tgctttatca
     6181 ggatccagtc caagtgcaat aaaatccata gcagcattta atgtattgtg tgccaacagg
     6241 cgggggtcgt gaatgctggt taatgcatga tagttaacaa taaagcagaa aagttcattg
     6301 ttatattgca attcgatcat gggtttcatc atggcaaaat agttacctat atgcaatgta
     6361 cccgatggtt gtattcctga aagtatcctt gacatttgta ccaccttttt aagtatggtt
     6421 attaatgttg tgtatcagaa tgtagacaga tgtaatagtc tgttttatat cgtcaatact
     6481 aattttaata acctgcctat aaaaaaattt tatttttgtt ttatttcatc aaaaacagtt
     6541 attttatgaa tggaaagtaa taaggtgtaa cgtatggcta ttgcaaattt tttacagtat
     6601 actactatga aaatgaaaaa ggtaacagtg ttgctatatt gcatattact gtctgcgata
     6661 gttactataa ctgttctttt cctgttattt a
//
LOCUS       DAOSOX010000127         6614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11699_length_6614_cov_7.179753, whole genome shotgun sequence.
ACCESSION   DAOSOX010000127 DAOSOX010000000
VERSION     DAOSOX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11699_length_6614_cov_7.179753"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..507
                     /locus_tag="PLX22_09035"
     CDS             277..507
                     /locus_tag="PLX22_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20079.1"
                     /translation="MLPRTTQLSILFAMIIAIIIVLLFEIPARPFTIRLAIWLYVLSL
                     LPGLGIAMLFDPAPREARERFLKINFYQEKKS"
     gene            525..1217
                     /locus_tag="PLX22_09040"
     CDS             525..1217
                     /locus_tag="PLX22_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT20080.1"
                     /translation="MDGTLIRTHGAGKKAMVQAAITVYGTAGDMDTVDFQGKTDPLIL
                     IQSLTPHGINQNTIFERMEDYKTLYFDLLQQTMPQSDAVLLPGIRELLHTLKKYDNLI
                     VGLLTGNFYKGAMIKLNYFDIFHYFTMGVFADDTYIRNEMPFVALQRLQAVTGTVFTT
                     EDLIIIGDTIYDIECGKTSGATTVAVATGWTDAETLLQHNPDYFFHELSDTNNFLYSI
                     KELQDCIQLHTQ"
     gene            1377..1877
                     /locus_tag="PLX22_09045"
     CDS             1377..1877
                     /locus_tag="PLX22_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT20081.1"
                     /translation="MQLGHVAVSFIIASYAPQITGGQMQAFSLESILVAFAAHWLPNL
                     DVIPIKLGWAKDSFHCTWSHSLVFAIAVFLLLLPFNSAWAMLSLVSLIVHYLADMPSS
                     VGLPLLMPFTKKRFTINLWADTGHSGWDALIGTYVQAWTWILEGGAFVFLFVRMYQLS
                     IWPFIS"
     gene            1906..3471
                     /locus_tag="PLX22_09050"
     CDS             1906..3471
                     /locus_tag="PLX22_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOT20082.1"
                     /translation="MKTIKSSYEYVIIGSGFGGSFAAYALAKAGKQVLVIERGVWAKR
                     DDTCWDEYALHLQQKPLYRGHTPYAVDQHGTLEYDWPDDTVGGMSTFYGAAAFRMRNE
                     DFEGPPAPDSDTRNAELAWPFRYDTLAPYYDKAEELQCVAGIQGQDITQPPRTKDYPQ
                     QPPLNLSRPSQKVWDAATSLGLHPFYIPMAINFSGVCNNHTCILCNTCDHYLCKIQAK
                     NDLSVSVLPRAMHYGAQVLDNHRAIKINFVKSKAQSVDVINQQTRQVMTIQCKYLIVA
                     SGPIATPHLLLASGITGYPNTNHIGHNLMRHINGVVSGVVPYIANPDNTLAKRIAISD
                     FYFGDSSGKMLPEGNWGIIQDIATPGKGVIKANAPFGLKNIAATFSQFLINQLVIAED
                     IPQYTNRIYCSDPRDIFGMPQVMIYHRYHSRDIQARNALYKQAKRILRKAGALFFYTM
                     PIVTFSHALGTCRMGNNKNLSAINPECRLWDIENCYITDASALPTGGSVNPSLTIAAN
                     ALRVTDIIVQSRG"
     gene            3476..4459
                     /locus_tag="PLX22_09055"
     CDS             3476..4459
                     /locus_tag="PLX22_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT20083.1"
                     /translation="MAQLPSICFFGCGYIAKRHAKLLRKLYPDITLGFSSLYQQDASE
                     YARMFNGTAFKSYEEAATSDYDIAFITTPHAYHKDLAILAANNKKHVIVEKPAARNLK
                     EFDAIIKAVTKNNVRFTVAENYYFKPFIKTIHRHIAKGSIGTPLYVRLTKHNHDIISG
                     WRTDARLMGGGALLEGGCHWVNELVSLSNATPEEVLAVKPQMQYPTSIPFEDTIHITV
                     KFNNGSFATLFHSWYVPNTLKGVALSKIYGTEGTVTFESNGLFTVVKGKRFAMNFSFR
                     DFLGFRAMHQSFIENYRTQKEWEPNWKRIRMELSLIEAAYRSLKTNKFESI"
     gene            complement(4525..5433)
                     /locus_tag="PLX22_09060"
     CDS             complement(4525..5433)
                     /locus_tag="PLX22_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOT20084.1"
                     /translation="MNKALILDIKGNSLDDGPGIRTVVFFKGCPLNCVWCHNPESKRA
                     TPELSWDGRECIGCYSCKNVCALNAIIPGSTHFINRAVCNLCFACVDVCPSGAMSKVG
                     KLMAVEDIIQTIIKDKPFFDTSGGGVTLSGGEPTVYMQFASELLKALKKIKIHTLIET
                     GGYFNGDEFEANIVPYCDMVYYDIKLIEDFKHKKYCGVSNAKILQNFKKLVELSQKNA
                     IQVMPRIPLVPGITATKHNIEGIAHFLRECGVNRVQLLRYNPLWPEKCFKIGSNGNID
                     KALTQWMSQKDYEFYAKILVKNTIQL"
     gene            complement(5430..>6614)
                     /locus_tag="PLX22_09065"
     CDS             complement(5430..>6614)
                     /locus_tag="PLX22_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT20085.1"
                     /translation="TAATPSMHNDAAIIKALTPMGYPINDIRDWSATGCVEPTLSHKH
                     FGHTNMQMMNMVAALEMTLNNGYHPLTRLDVGPKTGRPEDGVFTTFDTFFDAFTQQFA
                     FLIDQSIDYNNKLAEAHQLIRPTPLLSAFIEGCLEKGKDVTMGGAKYNSSGTAIIGLA
                     DVTDSMMAIKKLVYDEKRYAFADIKKAVDSNFAHDAVLLSLVHKKIPLFGSGSDEAVA
                     MANRITTWAHDHYSKMPHYRGGAYATGFWSMSNHVAFGTLTGALPSGRLAGKPFTPGL
                     TPQPFASKNLLDNIRDVARLNPENCNNNIAFNVKVVPSAIESRQKTVNDMFSYVKTYF
                     ELGGMQMQMNVVTSAVLRDAMAHPENYRNLIVRISGYNAYFVTLNRQMQLELIERAEY
                     GI"
BASE COUNT         1989 a         1430 c         1291 g         1904 t
ORIGIN      
        1 ctactataca taacgccaga gatgaaaaat attaaaaaga attgagcatt tttttatact
       61 ttttgtgatt tttttaaaaa acactaagta taggggtcag agcatatact gttaaaaaaa
      121 aaatcgctca aagtaacatg tagtttgctg attacacgga gaaaacaaaa taatgggggc
      181 agagcctttt ctattatatt ttttttattg attttatgtg ctataaactt ttattgttac
      241 aaacattgaa actatatcac aaataagggt tgcactatgt taccacgaac aacacaatta
      301 agtattcttt ttgcaatgat cattgctata attattgttt tactgtttga aattccggca
      361 cgtccattta ccatacgttt agcaatatgg ctttacgttc tttcattgtt accgggattg
      421 gggattgcca tgctttttga tcccgccccc cgtgaagcca gagagcgctt tttaaagata
      481 aatttttacc aggagaaaaa gtcataaaag caatactctt tgatatagat ggaacgctaa
      541 tccgcacaca cggtgccggg aaaaaggcaa tggttcaggc tgccatcaca gtatatggta
      601 ctgcagggga tatggatacc gtggactttc aggggaaaac cgatccattg atactgatac
      661 aatcgttgac accgcatggt ataaaccaga ataccatatt tgaacgcatg gaagattata
      721 aaacccttta ctttgatttg ttacaacaaa ccatgccaca gtcagacgca gttttacttc
      781 ccggtatacg ggaattgtta catactttga aaaagtatga caacctcatc gtaggacttt
      841 taacaggaaa tttttataaa ggagctatga taaaattaaa ttactttgat atctttcatt
      901 attttactat gggcgttttt gctgatgata catatatccg caatgaaatg ccatttgttg
      961 cactacaacg tctacaggct gttaccggta ctgtttttac aaccgaggat ctcataatca
     1021 ttggcgatac catctatgac attgaatgcg gtaaaacatc aggagctaca acagtagctg
     1081 ttgcaacagg ctggactgat gctgaaaccc tgcttcagca taaccctgat tattttttcc
     1141 atgagctatc ggacacaaat aattttttgt attcaatcaa agaattacag gattgtatac
     1201 agttacatac tcaataacaa tttggaaaca acgtaaagtc atcatgaaac atggtaagga
     1261 ttaaaaatct atgtgtcagg atattatgtt aacacacgat gccacaattg atgcaacatg
     1321 catcgtttaa acacaataca tttgacgcta ctatcttatt gttgctggag gaacacatgc
     1381 aattaggtca tgttgccgta tcatttatta ttgcatccta tgcaccgcag attaccggtg
     1441 gccagatgca ggcattttca ttagaaagta tactggtagc atttgctgct cactggttac
     1501 ccaacctgga tgttattccc attaaattgg gctgggcaaa agattcattc cactgtacat
     1561 ggtcgcactc acttgttttt gccattgcag tttttttgct tcttttgcca tttaacagtg
     1621 catgggcaat gctttcgctg gttagcctta ttgttcatta tctagccgat atgccaagca
     1681 gcgtaggact gccgctactt atgcccttta ccaaaaaacg ctttaccatc aacctgtggg
     1741 cagataccgg tcattcagga tgggatgctc tcattggcac ctatgtacag gcatggacat
     1801 ggatattaga aggtggcgca tttgtgtttt tatttgtgcg catgtatcag ctcagcatat
     1861 ggccatttat ttcttagggc gatagttacc gtaaattaac ctgaaatgaa aacaataaaa
     1921 tcttcgtatg aatatgtaat cattggcagt ggctttggag gttcatttgc agcgtatgca
     1981 ttagccaaag ccgggaaaca ggttcttgtt attgagcgtg gagtatgggc aaagcgcgat
     2041 gacacctgct gggatgaata tgcactgcat ttgcagcaaa aaccgttata tcgtggccat
     2101 acaccgtatg cagtggacca acatggcaca ctggaatatg actggcccga tgacacagta
     2161 ggaggcatga gtaccttcta tggagccgca gcattcagaa tgcgcaatga agactttgaa
     2221 ggaccgccag cacctgattc tgatacgcgc aatgcagagc tggcatggcc atttaggtac
     2281 gatacattag ccccatacta tgataaagca gaagaactgc aatgtgtagc aggcatacag
     2341 ggacaggaca taacacaacc gccccgtaca aaggactatc cacagcaacc accattaaac
     2401 ctttcacgcc cttcgcaaaa ggtgtgggat gctgcaacct cattagggct gcatcctttt
     2461 tatatcccca tggcaattaa ttttagcggc gtgtgcaaca accatacatg catactgtgc
     2521 aacacctgcg atcactatct ttgcaaaata caggcaaaga atgacctctc ggtaagcgtt
     2581 ctgccccgag caatgcacta tggcgctcaa gtactggata atcaccgggc aataaaaatc
     2641 aactttgtaa aaagtaaagc tcaatcggtt gatgttatca atcagcaaac acgccaggtc
     2701 atgaccatac agtgtaagta tttgatagta gcttcggggc ccattgcaac accccattta
     2761 ttgctggcat ccggaattac cggttatcca aacacaaacc atattggaca caaccttatg
     2821 cgtcatatca acggcgtcgt gagcggtgtt gtcccctaca ttgccaatcc tgataataca
     2881 ctggcaaagc gcatcgcaat ttctgatttt tattttggcg atagttccgg taaaatgttg
     2941 ccagaaggta actggggcat aatacaggat atagccaccc ccggcaaagg cgtgataaaa
     3001 gccaatgcac cgtttggatt aaaaaatatt gcagcaacat tttcacaatt tcttataaac
     3061 cagcttgtca ttgctgaaga tattccacag tataccaaca gaatctattg ctctgacccc
     3121 agagatatat ttggcatgcc tcaggtaatg atataccatc gctatcatag tcgcgatatt
     3181 caggcacgca atgcattata caaacaggca aaacgtatat tacgcaaagc aggtgcactg
     3241 tttttttata ccatgcctat tgttactttt tcccacgcat tgggaacatg cagaatggga
     3301 aacaataaaa acctttcagc cattaatcca gaatgcagat tatgggatat tgaaaactgc
     3361 tacataaccg atgcaagcgc cctgccaacg gggggttctg ttaatcccag cttaacaata
     3421 gccgcaaatg cactgcgagt gacagatatt attgtacaat caaggggata acatcatggc
     3481 acagcttcca tcaatctgct tctttggatg cggatatatt gcaaagcgtc atgctaaact
     3541 attgcgcaaa ctataccccg atattacact gggcttttca agcctgtatc aacaggatgc
     3601 atcagaatat gcacgcatgt ttaacggaac agcatttaaa agctatgaag aggcagccac
     3661 atcggattat gacattgcgt ttattacaac acctcatgcg tatcataagg atttagcaat
     3721 attagctgca aacaacaaaa aacatgttat agtagaaaag ccagcagcac gaaacctcaa
     3781 agaatttgat gcaataatca aagctgtaac caaaaataac gtccgattta cagttgccga
     3841 aaattattac ttcaagccat tcataaaaac aatacacagg cacattgcaa aaggctccat
     3901 tggcacacca ctgtatgtgc gattaaccaa gcataaccat gacataatct cgggatggcg
     3961 caccgatgcc cggcttatgg gtggcggcgc actcttagaa ggcggctgtc actgggttaa
     4021 tgagcttgta tcactgagca acgcaacacc agaagaagta ctggcagtaa aaccacaaat
     4081 gcagtatcca accagtatac cctttgaaga taccatccac atcactgtta aatttaacaa
     4141 cggtagtttt gctacattat ttcattcgtg gtatgtcccc aatacattga aaggtgttgc
     4201 tctttcaaaa atatatggta cggaaggcac cgtcaccttt gaaagcaacg gactcttcac
     4261 tgttgtgaag gggaagcgct ttgcaatgaa tttttctttc cgcgattttt taggctttcg
     4321 cgccatgcac caatcattta tcgagaacta tcgcacacaa aaagaatggg aaccaaactg
     4381 gaaacgcata cgcatggaat tgtcattgat tgaagccgca taccgttcgt taaaaactaa
     4441 caaatttgaa agtatataac agcactctgc acatctttat gaaaaaacta tcatgtatgt
     4501 gttatgattt tctattatcc catactataa ttgtatagta tttttcacta atatttttgc
     4561 atagaactca tagtcttttt gcgacatcca ttgtgttaat gctttgtcaa tattgccatt
     4621 actccctatc ttaaagcatt tttcgggcca taatggatta taccgtagca gttggacacg
     4681 gttaacaccg cattcgcgta aaaaatgcgc aataccttct atattatgct ttgttgccgt
     4741 tattcccgga acaagtggta tacgtggcat tacctgaatg gcattttttt gtgacagttc
     4801 aacaagcttt ttaaaatttt gcaaaatttt tgcatttgaa accccacaat attttttatg
     4861 cttaaaatct tcaataagct tgatatcata atacaccata tcacaatatg gtactatatt
     4921 ggcttcaaat tcatccccgt tgaaataacc gcctgtttca atgagtgtat ggatttttat
     4981 tttcttcaat gctttcaaca attctgatgc aaattgcata tatactgttg gttcgccacc
     5041 cgatagcgta accccgccgc cggatgtatc aaaaaaaggc ttatccttta taatagtctg
     5101 tataatatct tctacagcca ttagtttgcc aactttactc attgcacctg aagggcatac
     5161 atcaacacag gcaaagcaaa gattgcatac agcacgatta ataaaatgcg tacttccggg
     5221 aattattgca tttagcgcac acacgttttt gcaactgtaa cacccaatgc attccctgcc
     5281 gtcccaggaa agctctggcg ttgcccgctt gctttcggga ttatgacacc atacgcagtt
     5341 gagcggacat cccttaaaaa agacgacggt tcgaatgccc ggaccgtcgt ccagagaatt
     5401 tcctttgata tcgagaatca gcgctttgtt catataccat attcagccct ttcaataagt
     5461 tcaagctgca tctgacggtt caatgtcaca aaataggcat tgtatcctga aatacgtaca
     5521 atcaggttgc gatagttctc gggatgagcc attgcatcgc gcagcacagc cgatgtaaca
     5581 acattcatct gcatctgcat gccgcctaat tcaaaatagg ttttcacgta tgaaaacatg
     5641 tcattgactg tcttctggcg cgattcaatt gcagagggca caaccttcac gttaaacgca
     5701 atattgttgt tacaattttc aggattgagc cgtgcaacat cccgaatatt atcaagcaga
     5761 ttttttgaag caaacggctg aggagttaat cctggtgtaa acggctttcc cgccaatctt
     5821 cccgacggca gcgcaccggt aagcgtacca aaggcaacgt gatttgacat ggaccagaat
     5881 ccggttgcat atgctcctcc acggtaatga ggcattttag aataatgatc atgcgcccat
     5941 gtagtaattc tgttagccat agccacggct tcatcactgc ccgagccaaa aagaggaatt
     6001 tttttatgca ccagcgaaag cagtacagca tcatgtgcaa aattactatc aacagctttt
     6061 tttatatcag caaacgcgta tcgtttttca tcatatacca gctttttaat tgccatcatg
     6121 gaatcggtaa catcagccag ccctataatt gccgtacccg agctattgta ttttgcaccg
     6181 cccatggtaa catccttacc cttctcaaga catccctcaa taaatgctga taaaagcggt
     6241 gtcgggcgta ttaattggtg ggcttctgcc aacttattat tgtagtcgat agattgatca
     6301 attaaaaacg caaattgttg tgtaaacgca tcaaagaacg tatcaaacgt tgtaaatact
     6361 ccatcttccg gcctgcccgt ttttggtcca acgtcaagcc ttgtaagcgg atgatagcca
     6421 ttattcagtg tcatctccag tgctgctacc atattcatca tctgcatatt ggtatggcca
     6481 aagtgtttgt gcgatagtgt tggttcaaca cagccggttg ctgaccagtc tcgtatatcg
     6541 ttaataggat atcccatggg ggtcagagcc ttgatgatag cagcgtcgtt gtgcattgat
     6601 ggtgtagcag cggt
//
LOCUS       DAOSOX010000128         6546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11863_length_6546_cov_8.280080, whole genome shotgun sequence.
ACCESSION   DAOSOX010000128 DAOSOX010000000
VERSION     DAOSOX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11863_length_6546_cov_8.280080"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLX22_09070"
     CDS             complement(<1..489)
                     /locus_tag="PLX22_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20086.1"
                     /translation="MTLQPEEMLEEWDWEKGIPTGKSIPRSIAHQCGIAHEGVHLWIV
                     RSIPIPQLVFQKRASHKDMYPGYLDITVGGHVPYGLDTNKIHKEAYEEIGIIPDDNKL
                     IDLGYFRYEENNATLFHREFQRVYLYMSDEPLTAYSFNDNEVNAVYAIPVDYCKAIFT
                     DDY"
     gene            942..1703
                     /locus_tag="PLX22_09075"
     CDS             942..1703
                     /locus_tag="PLX22_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20087.1"
                     /translation="MLFFIASSIAIFFQYKKVTVDINDFVVEVRNISKTLITMYSDAA
                     GLAYTTKSTYKLVSLFHQQQSLRGIKEIFFVLDNGTIVAHSDETKEKELHGNITNDEF
                     RYNIDMILYPYSIQSKQLFIYDYFMPDITIPYSKDIRHLIKRFIYKDIDTNSWLVSKA
                     VFVNGKSIGTVNCIIVKEPVYQIIDSSFYESMVIIAIIGIISFFTSLIAGVIVYTRQK
                     KVCKTLLYDIGLKLHHEGGSHIPHTGIKEAIPVKE"
     gene            1710..2291
                     /locus_tag="PLX22_09080"
     CDS             1710..2291
                     /locus_tag="PLX22_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT20088.1"
                     /translation="MVIQHRVEHSIAVVTVQGYINHTNAVEFEEYISSLTKECSSAII
                     DCSQLEYLSSTGIGALMYAERILNSIGGILVVCNCNSEVKTLLDMVSLRISIFDSVDL
                     AMQAVNTILQRLPNQNISEQATGETHEDKHYTEEKQKVVPDHGSIMFDHPIVVECPKC
                     KSMVKVNASGKFKCPECNLKFTVDDDRTIYFDV"
     gene            2387..2932
                     /locus_tag="PLX22_09085"
     CDS             2387..2932
                     /locus_tag="PLX22_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20089.1"
                     /translation="MKVIHVLKRIEMIDQDIRELRKLEKSLQSNKSFTQPIFITIEKQ
                     INILLGERIKLIELQIANPPANLQPEEEEIKEPLVAVPKKKGNKKKEQKMHKSKNALS
                     ELDEEAFNEQEFSMLTQDDIDKKIATMQEKIPSTLDDHDDTDVKLLDIALEKGTINKQ
                     EVETQKKKIRFFKDNFPGGES"
     gene            complement(2939..>3576)
                     /locus_tag="PLX22_09090"
     CDS             complement(2939..>3576)
                     /locus_tag="PLX22_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20090.1"
                     /translation="YSFYGDSFYLQALHTLYNKRIQKGITIGYIEENDRLQVHIINEK
                     GNIFTFFAPKNSKMIFLYHIYGFCTNVLKRIPSSSRESQSSIICYCVQTDRRGGINMV
                     DETGKIHGDYLLNFTKSKALRVQVARHMGNEIFYNFIFPDNVSSGFMTVKELYSVKHK
                     ITDLKKTGIPIYSYIGELIFNDLNQKEALLGSSIYFVEKYKLELMLQRIHN"
     assembly_gap    3577..3676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3677..5817)
                     /locus_tag="PLX22_09095"
     CDS             complement(<3677..5817)
                     /locus_tag="PLX22_09095"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HOT20091.1"
                     /translation="MDVLQTISENKDKFIAFNNEKFKRFQQLIPNPAMRRIVNTIPFL
                     LCINNKKMPGFVEGEVPLGIKNFTLDEDTMRYLHGRYPGTTFHDFERGDFIKMFAVMG
                     SVGSVAYNKKSDLDYWACIHRNSVTQEAFDNFKKKVTIVQEWASKELEVPVHIFINDI
                     ESVKNNIFAEDEEEAFGTTVGAVLKDEFFRSSIIIAGKIPFWWVVPSFIHDDEYDTLY
                     NQLPEEMKRDDYIDLGNLFEISKEDFLGAALFQIIKSLGNPFKSILKIGVLEKYLFGK
                     TDSPLLSQKVKMHVLLSRLTNSILDSYLLMFDEVYDYYSQVINDPALLQILKQNLYLK
                     IDPQLSRYLGIKDKKNIPYKVAVMFKYVHDWGWNLKIIEDMDNFDNWDFQRVMHFWDT
                     VKRFMLMSYQKISAQFNTLNLKKQMSESDFILLSRKIKSYFSVEENKFEKLITFKDTP
                     AEPILYIEPINEGIHEVKWRLFKRNRSEKNVFTTTTLRTENDLVKLLVWLTVNNVYDP
                     TITRINIQSGYVRINQNLVVELLNQMSLFFAGKEKIKNKYYLKSPFTMLHCIIINFNN
                     EAVDHLDSLYHVYSTSWGEQFIKRYTTIAHITPILQTMLQDGLELSLPFDDYCLINSP
                     GTHKRFCKNFIQLFKESYKAIVTGKTPHCRFIGELEGIYFAALRNTTVEVQAFDNIVK
                     VLTTLTLKPAVETHYSFYGDSFYLQALHTLY"
     gene            complement(5830..>6546)
                     /locus_tag="PLX22_09100"
     CDS             complement(5830..>6546)
                     /locus_tag="PLX22_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20092.1"
                     /translation="HSMFRDCVANIPDASYVIFDTQENKPPSLIPADSMKSIQAVQFY
                     RPFAENCKTYVYKKCNSLKLTITEEAADHLVEFTASNIKEIDDILNLISFSETIDITI
                     EFLEGFLINERGSIEFEFVDKFFLKDKNALILFQEALQRNINQVLLLTLLFNHAQKIE
                     AYHIKTQKGSTPESLMKELKIYGKRQKVFAKQSIVFNLESIRKFIAKVYVYDHKLKSY
                     RFKGIFQENPMYDLLLQYLV"
BASE COUNT         2125 a         1080 c         1117 g         2124 t
ORIGIN      
        1 atagtcatcg gtaaatatag ctttgcaata atctacaggt atagcataga ccgcgtttac
       61 ctcattatca ttaaatgaat aggctgtcaa cggttcatct gacatataca aatataccct
      121 ctggaattcg cggtgaaaaa gagtagcatt gttttcttca taccggaaat atcctaaatc
      181 aatgagttta ttatcatccg gtattatccc tatctcttca tatgcctctt tatggatttt
      241 attggtgtct aatccatagg gtacatgccc accaacggtt atatctaaat aaccaggata
      301 catatcttta tgggaagcac gtttctggaa cacaagctgt ggaataggta tagaacgcac
      361 aatccataaa tgaacacctt catgggctat gccgcattga tgggcaatac ttcgtggtat
      421 agattttcct gttgggatac ctttttccca atcccattct tctaacattt cttctggttg
      481 tagtgtcatt gtaattatgt tccccctctg taaatagaat actgtgtcat tttgagggat
      541 gtagttcaga agaatctgtt cttttaaaaa actacgagat tcttcacttc gctcacaatg
      601 acattcccgt atcacgggct tcttcacttt gactttgctc agtacaggcg gctttacctc
      661 agaatatcag taacactaca tgttcattat atcaattcat caacaaactt ctaaaaactg
      721 gattgtaata ttgtatgggt acatgctatg ctcctcagtt gtttgccatt agttgacatg
      781 ctatcaaagc aactttcact atacctataa aaattgtata tatcaatatg atattttccg
      841 atgaatacta tagtatatac tggtaaccgt tattgtaaag agttatgaat aaaaaaaaca
      901 ttataaggtt tccataccta tttgtgatgc tattgtttgt tatgctgttt ttcattgcaa
      961 gcagcattgc tatttttttt caatacaaaa aagtaacagt tgatattaac gattttgttg
     1021 tagaagtgcg caatatttcc aaaacactta ttacaatgta ctcagatgca gcaggtttag
     1081 catatacaac aaaatcaaca tataagcttg tgtcattgtt tcatcagcaa caatcactga
     1141 ggggaataaa agaaatcttt tttgtgctgg ataacggtac aattgtagca cactcggatg
     1201 aaacaaaaga gaaagagctt catggtaata ttacaaatga tgaattcagg tataatattg
     1261 atatgatctt atatccttat tctatacaat caaagcaact ttttatatat gattatttta
     1321 tgcctgatat aacaatcccg tacagcaaag atatacgtca cttgataaag cgctttattt
     1381 ataaggatat tgataccaat agctggttgg tatcgaaggc tgtttttgtt aatggaaaat
     1441 caatagggac agttaattgc attattgtca aggaaccagt ataccagata attgatagct
     1501 cattttatga aagtatggtg attatagcta ttattggtat aatttctttt tttacttcac
     1561 tcattgctgg tgttattgta tacaccaggc aaaaaaaggt atgcaaaact cttttgtatg
     1621 atattggatt aaagttacat catgaagggg gaagccatat tccccatact ggcatcaaag
     1681 aagctatacc ggtaaaggag taggctatta tggttattca acatagggtg gaacattcga
     1741 tagcagtggt tacagttcag ggctatatta accatacaaa tgcagtagaa tttgaagaat
     1801 atatatcatc gctgacaaag gaatgttcat ctgctattat tgattgctcg cagttagaat
     1861 atctcagttc aacaggaatt ggagcattga tgtatgcgga aaggatacta aattctatag
     1921 ggggtatact ggtagtatgt aattgcaata gtgaagttaa aacattactt gacatggtgt
     1981 cactgagaat atcaattttt gattcggttg accttgcaat gcaagcagta aacactatcc
     2041 ttcaacggtt acccaaccag aatatctccg aacaggcaac aggggaaact catgaagaca
     2101 aacactatac agaagaaaaa caaaaagttg taccagatca tggttcaatc atgtttgatc
     2161 accctatagt agtagaatgt ccaaagtgca aatccatggt taaggttaat gcttcaggta
     2221 aatttaaatg tcctgaatgc aatctcaaat ttactgtaga tgatgacaga actatatatt
     2281 ttgatgttta aaaactatag caaaataaaa gtttattgac attattttaa ttatagattc
     2341 actattacat acagcagtat taatttcttt taattgaggt ccagctatga aagttattca
     2401 tgtgcttaaa cgtatagaga tgattgatca ggatattcgt gaactgcgaa aactggaaaa
     2461 gtcgttacag agcaataaat catttacaca accaatattt atcacaatag aaaagcaaat
     2521 aaatattttg ttgggtgaac gcataaagtt aattgaattg cagatagcca acccgccggc
     2581 taatttacaa ccagaagaag aggaaatcaa ggaaccgctt gttgcggtac caaaaaagaa
     2641 aggtaataaa aagaaagaac aaaaaatgca taaatcaaaa aatgctttat cagaacttga
     2701 tgaagaggca ttcaatgagc aggaattttc tatgcttacg caggatgata ttgataaaaa
     2761 aatagctaca atgcaggaaa aaataccgtc cacattagat gaccatgatg atacggatgt
     2821 caaattactt gatattgctc tggaaaaagg aacaataaac aaacaggaag tggaaacaca
     2881 gaaaaaaaag atacgattct ttaaggataa cttcccgggt ggcgaatcat agtttgtatt
     2941 aattgtgtat acgctgtagc atcaattcca atttgtattt ttcaacaaaa taaatacttg
     3001 accctaataa agcttctttc tggtttaagt cattaaatat cagttcacca atatatgaat
     3061 agattggtat gcccgtcttt tttaaatcag taatcttgtg tttgacacta tatagttcct
     3121 taactgtcat gaaacctgat gatacattat cgggaaaaat aaaattgtaa aatatttcat
     3181 ttcccatatg cctggcaacc tgaacacgta aagcttttga ctttgtgaag ttaagaagat
     3241 aatccccgtg tatcttgcca gtttcatcaa ccatgttgat gcctcccctg cgatctgttt
     3301 gaacacaata acaaatgatg ctggactgac tttctcttga tgagcttggg atacgtttga
     3361 gtacattggt acaaaatcca tagatgtggt ataaaaaaat cattttacta ttctttggtg
     3421 caaaaaaggt aaaaatattt cctttttcat tgattatgtg tacctgtaat cggtcatttt
     3481 cttcaatgta tcctatcgtt atgcccttct ggatgcgttt gttgtaaaga gtatgcaatg
     3541 cctgaagata aaaagaatca ccataaaatg aatagtnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnttgt aaagagtatg caatgcctga agataaaaag aatcaccata
     3721 aaatgaatag tgtgtttcaa cagcgggttt taaggtaagt gtcgttaaaa ctttgacaat
     3781 attgtcaaat gcctgcacct caacagtagt atttcgcaat gcagcaaaat agataccctc
     3841 aagttcacca ataaatcggc aatgaggcgt tttgccggta actatcgcct tatatgattc
     3901 tttgaaaagc tgaatgaaat ttttacagaa acgcttatgc gtcccaggac tgttgataag
     3961 acagtaatcg tcaaaaggga gagataattc caaaccgtcc tgcagcatgg tttgtaaaat
     4021 aggggtgata tgggctatag tagtataccg ctttatgaac tgctctcccc agcttgttga
     4081 ataaacgtga tataacgaat caaggtggtc gactgcttca ttgttaaaat tgattataat
     4141 acagtgaagc atggtaaaag gagattttaa ataatattta tttttaattt tttcttttcc
     4201 tgcaaaaaaa agtgacatct gattgagaag ttcaacaacg agattctggt ttatacgcac
     4261 ataacctgat tgtatattaa tacgggtgat tgtaggatca taaacattgt taacagttaa
     4321 ccataccagc aatttaacaa ggtcattttc agtgcgcaac gttgttgtgg taaaaacatt
     4381 tttttcactg cggtttcgtt taaaaagacg ccactttact tcatggatgc cttcattaat
     4441 aggttcaata tacaggatgg gttccgcagg tgtatcttta aatgtgatga gtttctcaaa
     4501 tttattttcc tcaacagaaa agtatgattt gattttccgt gacagtaaaa taaaatctga
     4561 ttcggacatc tgctttttta gattcagggt gttgaattga gctgatattt tttgatagct
     4621 catgagcata aatcgtttta ccgtgtccca gaaatgcata acccgctgga agtcccagtt
     4681 gtcaaaatta tccatatctt caataatttt cagattccag ccccagtcat gaacatactt
     4741 aaacatcact gccactttat aaggaatatt ctttttatcc ttaatgccta aatatcttga
     4801 taattgggga tcaattttta gataaaggtt ttgttttaaa atttgaagga gagcagggtc
     4861 attaatgacc tggctatagt aatcatatac ttcgtcaaac atcaataaat atgagtcaag
     4921 tattgaattg gtaagccggc ttaacaaaac atgcatttta actttctggc ttaatagtgg
     4981 cgaatcggtt ttaccaaaaa gatatttttc caaaacgcca atctttaaaa tagatttgaa
     5041 tggattaccc agtgatttaa taatctgaaa taatgctgct cctaaaaagt cctcctttga
     5101 tatctcaaat agattcccta aatctatata atcatcgcgt ttcatttctt caggaagttg
     5161 attataaagc gtatcatatt catcatcatg aataaaactt ggaacaaccc accagaacgg
     5221 gattttaccg gcaatgatga ttgaacttct aaaaaattca tctttaagta cagcacctac
     5281 ggtagtgcca aatgcttctt cttcatcttc agcaaatata ttatttttaa cactttcgat
     5341 atcgttaata aaaatatgta ctggtacttc cagctctttt gatgcccatt cctgtacaat
     5401 ggttactttc tttttaaaat tatcaaaagc ctcctgagta actgaatttc tatggatgca
     5461 tgcccagtaa tcaaggtcgg attttttatt gtatgctacc gagccaacac ttcccatgac
     5521 ggcaaacatc tttataaaat caccccgttc aaaatcatgg aaggtggtgc ctggatatct
     5581 tccatgcagg taacgcatgg tatcttcgtc cagcgtaaaa ttttttattc ccagtggcac
     5641 ttcaccttct acaaaaccag gcatcttttt gttattaatg catagtaaaa agggtatggt
     5701 attgacaatt cgtcgcattg cgggattggg gataagctgc tgaaaccgct taaatttttc
     5761 attgttaaaa gcaatgaatt tatctttatt ctcggaaata gtttgtaaca cgtccataca
     5821 ttaatagttt cacactaaat attgcagtaa aaggtcatac atagggtttt cctgaaaaat
     5881 acctttaaaa cgataacttt taagtttgtg atcgtaaaca tatacttttg ctataaattt
     5941 tcgtattgat tcaagattaa atactataga ttgttttgca aatacttttt gtctttttcc
     6001 atatatcttg agttctttca tcaaagattc cggggtgctt cccttttgcg tcttgatgtg
     6061 atatgcttca attttctgag catgattaaa taacaaagtt aatagtaaca cctggtttat
     6121 gtttctttgc aacgcttcct gaaacagaat taatgcattt ttatctttta aaaaaaattt
     6181 atcaacaaat tcaaactcta ttgatccgcg ttcattgatt aaaaatccct ctaaaaattc
     6241 aatggttata tctatagttt ctgaaaatga gataagatta agaatatcat caatttcttt
     6301 aatattgctg gcagtaaatt ctactagatg atctgcagct tcttcggtga tggtgagctt
     6361 gaggctattg cattttttat aaacatatgt tttacaattt tcggcaaatg gcctgtaaaa
     6421 ttgtacggct tgaatacttt tcatactatc agcagggata agtgatggag gtttattttc
     6481 ctgggtatca aagataacat aacttgcatc ggggatattg gctacgcaat ccctgaacat
     6541 ggagtg
//
LOCUS       DAOSOX010000129         6541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11882_length_6541_cov_9.576164, whole genome shotgun sequence.
ACCESSION   DAOSOX010000129 DAOSOX010000000
VERSION     DAOSOX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11882_length_6541_cov_9.576164"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1829
                     /locus_tag="PLX22_09105"
     CDS             192..1829
                     /locus_tag="PLX22_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT20093.1"
                     /translation="MMGKYKVKYFIIIFWILIFPVALYFFDKYIPVNISLNLLILLFF
                     IALLLYAIYVYSNKKIRIFIIGLLIISIIPSFFIISAFSITNTIYHRSDFDVIFTTDL
                     DESLEFIHQFVNFKIIMLLIAYFGPMPLFFKMKDIQMELNNNKRKLIVMGSFLVLILI
                     FTVNWKLLQKEYHVVDFYKSYYQFINNKNYEIIKAKRADLKWKDKVTCDLKIREHKLY
                     VLVIGESLSRAHMQIYGYQRETNPQLIKIKDELYIFNNVISPAVTTVDVMKYVLSFAN
                     LDHPEYFIEKRNVVNLFNDVGFETIWIGNQSLRGNRNNVSHSIVARECNQYYDVSSET
                     DEVVINKLDTVLTDEPHKNLFIIIHLRGCHTKYSSRYSDIFNYFNHKKIPIPYDYGLD
                     DKQKLIIDEYDNSVRFNDYIIYSIIKRVKSHCKYAWVVYFSDHGEELFEFRNMFGHNS
                     RNFSKYMCEIPFIVWVSNEYKNANKSFFEDIPKYLNRAYSTENVIYSIADLSKLKFTD
                     FDASLSIFSKQYTNHLRKVYNIEYEKIPPITTVNGFK"
     gene            1971..3332
                     /locus_tag="PLX22_09110"
     CDS             1971..3332
                     /locus_tag="PLX22_09110"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOT20094.1"
                     /translation="MPLKVKYDFNDLMEKLGGKAMPLCNFCGTCIGVCASNALSADYE
                     KGRPVFDISKCTTCSLCYNNCQGAGVNFSELDNHFRLGERYDNYLGSYKKCFLGHSLD
                     DMIRIQGASGGCVTRLLVYMLEAGVVDGVILPRQKADQPWLFKPEIITNVETVKASAQ
                     SKYCLIPTNEILKTLKGKKGRYAIVALPCQIHSLRKLQHEHNKNAQKIEYILSLICGH
                     NMEYGATTFGMKKLNIKKEDVVDIKYRDYGDWPGGLSFYLKNGERKGINFFNYHYLNA
                     VYLPNRCRLCPDYYGNYSDISFGDSWLLRLLGRGMNEKGIPKGWSSIIARSQKGLSLL
                     EAAQKAHALYLEEIEPPEIHESFPFNISYKLNGIFVRLQFAKKKPEFIGLAKPEKLDR
                     YLYHFIYNIILILGSTSVFKFFLSIMPLNFNLFLIKNFKKLMGYKPNARDRVLQYNEK
                     SNV"
     gene            3424..4224
                     /locus_tag="PLX22_09115"
     CDS             3424..4224
                     /locus_tag="PLX22_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT20095.1"
                     /translation="MSEQYYFTLKGFWKRVKQEYDYYTRRPWTLKQVGEFWDTVNDYD
                     AVNEQLYTYHRRFTNSYELAYKHLKKDTYNMIDIQARSGKGSLFWHEKGKVTSSVCVD
                     FSDFLISLADKRLNKTGLQYSIVKVLNFPLPFNDGQFDLVCSYETIEHIYDYSAFFDE
                     LVRIMSKDGIMILTCPNRMWEWVHWLSAAININHSEGPHRFLKRGELIQCIKKNRMEI
                     LEENSTIILPFNNKLSIAINTFLENYLPEFIKRIIALRRTFILGKGDK"
     gene            4233..4877
                     /locus_tag="PLX22_09120"
     CDS             4233..4877
                     /locus_tag="PLX22_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT20096.1"
                     /translation="MKKFTLYLLVCFLVYFAIFVIIDFYAEKKYDILYNTINTPFDAA
                     VVFFSDFETHNTIDDESLRRLSFAIELYKQHAINNIIFCGGWRPSINTSGSKLMATMA
                     EEMGVDRSHIYYETHSRDTVQNWKEAEKIIVVKNFKKIILLSSPLHLMRIEKIVTIHD
                     DIDVTYSSYSKRSIIPPKGYLDNFCDYNYNMVSIISYYLLPSELYQYIVTNLRN"
     gene            4925..5899
                     /locus_tag="PLX22_09125"
     CDS             4925..5899
                     /locus_tag="PLX22_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT20097.1"
                     /translation="MSKKINYLNGIGIILLAIILWKIDFKQVFKYLKDANYWYLLLAY
                     PFILMNIFLKAVRWNFLMKTQGVRNSIFESFYVYLWAFYFGAVTPGRIGEISKALYYQ
                     EKFDNFGSSLSPVVVDRLYDVGIRIAMIFILYPFYSHLFHLKYVDFIILLSCIIAGIV
                     VLARYKSIQNIIITFSKFLVPKKYYATIENNISGFINDIIKMVTTVKYLAICIVITIP
                     SFLCYCMVGYFILKSFGINMTFTYNIFCLAVSSFSVAVPISVSGLGVREAVMIFLFNS
                     IGLGRESAVLFSLSIFALSPILGFHGWLVNLIMLIRNHIKKRMKVKVN"
     gene            6020..>6541
                     /locus_tag="PLX22_09130"
     CDS             6020..>6541
                     /locus_tag="PLX22_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT20098.1"
                     /translation="MKSGLTIIVPVYNEISAIEHSINHLKEIKKKCKNIDMEIIIVND
                     GSTDGTEKILARMPNNKDIKVINHSENQGYGAALKTGIRASKHQYVAITDADGTYPDE
                     RIPEFFEIVINEGLDMLVGARIGESVHIPFIRKLPKWVINKMANYMVGTKIPDLNSGM
                     RIMKKDVVKKYFKI"
BASE COUNT         2303 a          826 c         1118 g         2294 t
ORIGIN      
        1 tattttaaat tctatttgaa gaatccacat atcaatgaca tggaaaataa ggtaaactat
       61 ttgatgtaaa ttgcttgacg atatgtataa ggtaattatg aaaacaaaaa tattgttatt
      121 ctgaaaaatt tttatactat atagttttga tatgcaattc tttaactttg aataaagtga
      181 ggtaaaacgt aatgatgggt aaatataagg tgaagtattt tataataatt ttttggatat
      241 taattttccc tgttgccttg tatttttttg ataaatatat ccctgttaac atttctttga
      301 atttattaat tttattgttt ttcatagctt tacttttata tgccatctat gtttattcga
      361 ataagaaaat caggatattt ataattggtt tattgataat ttcaataatt ccatcatttt
      421 ttataatttc tgctttttct ataactaaca caatttatca tagaagtgat tttgatgtta
      481 ttttcacaac ggatttagat gaatcattag aatttattca tcagtttgtc aattttaaga
      541 ttatcatgtt acttatagca tattttggcc ctatgccatt gttttttaaa atgaaagata
      601 tacagatgga attaaataat aataagagaa aattgattgt aatgggttct ttcttggttt
      661 tgattttgat tttcacagtg aattggaaat tattacaaaa ggaatatcat gtagttgatt
      721 tttataaatc atattatcag tttatcaata ataaaaatta tgaaataata aaagccaaga
      781 gggctgacct taaatggaaa gataaggtga catgtgattt aaaaatacga gaacataagt
      841 tatatgtctt agtaataggt gaatcgcttt cacgagcaca catgcagata tatggatacc
      901 aacgagaaac aaatccccaa ttaataaaaa ttaaagatga attatatatt tttaataacg
      961 tcatatcacc agctgtaaca acagtagatg ttatgaaata tgttttaagt tttgctaatt
     1021 tagatcatcc cgaatatttt attgaaaaga gaaatgtggt aaatcttttt aatgatgtgg
     1081 gatttgaaac tatatggata ggaaatcaaa gtttgcgggg taacagaaat aatgttagcc
     1141 atagcattgt tgctcgagaa tgcaaccaat attatgacgt ttcatcagaa accgatgaag
     1201 ttgttataaa taaacttgat acagtgctta ctgatgaacc acataaaaat ttgtttataa
     1261 taattcatct gaggggatgc catacaaaat atagttcacg gtattccgat atctttaatt
     1321 attttaatca taaaaaaatc cctatcccat atgattatgg attagatgat aaacaaaaac
     1381 ttatcattga tgaatatgat aatagtgtaa ggtttaatga ttatattatt tactcaatta
     1441 ttaaacgtgt aaaatctcac tgtaaatatg catgggtagt ttacttttct gatcatggtg
     1501 aagaactttt tgaattcaga aatatgttcg ggcataatag tagaaacttc tcaaagtata
     1561 tgtgtgaaat accatttatt gtatgggttt caaatgagta caaaaatgct aataaaagtt
     1621 tttttgaaga catcccaaaa tatctaaata gagcatatag cactgaaaat gttatatata
     1681 gtatcgcaga tttatcaaaa ttaaaattta ctgattttga tgcaagtctt agtatatttt
     1741 caaaacaata tacgaatcat ttgagaaagg tttataatat tgagtatgaa aagattcctc
     1801 caattacaac tgtaaatggt tttaaataag ggacaatcat tgattaagaa tttattagtt
     1861 gactttattg tagctgaatt aaatttagaa gcaacaacaa atttaatttc ttattttcat
     1921 tgcgcttttc tattgattaa aacaaaaaca cagaaaggag tattccttta atgccattga
     1981 aagtaaaata cgactttaat gatctcatgg aaaaactggg tggcaaagca atgccgcttt
     2041 gtaatttttg tggtacctgt ataggggtat gtgcttccaa tgccctttct gcagattatg
     2101 aaaaagggcg tccggtgttt gatatatcta aatgtactac ctgtagctta tgttataata
     2161 attgtcaggg tgcaggtgtt aattttagcg aacttgataa tcattttaga ttaggtgaaa
     2221 gatatgacaa ctacctggga agttataaaa agtgtttttt gggacattca ctggatgata
     2281 tgataagaat acaaggagcc agtggtggtt gcgttacccg attattggtg tatatgctgg
     2341 aggcaggagt ggttgacgga gtaatacttc cccgtcaaaa agctgatcag ccatggttgt
     2401 ttaaacctga aataattact aatgtagaga ctgttaaagc atctgctcaa tcaaagtatt
     2461 gccttatacc aaccaatgaa attttaaaaa cgctaaaagg gaaaaaagga agatatgcaa
     2521 tcgttgctct tccatgccag atacatagtt taagaaaact tcagcacgag cataataaaa
     2581 acgctcaaaa aattgaatat atattatcgt tgatttgtgg gcataatatg gagtacggtg
     2641 caacgacatt cggcatgaaa aaacttaata ttaaaaaaga ggatgtcgtt gatataaagt
     2701 atagggatta tggtgattgg cccggtggac tttcttttta tcttaaaaat ggtgaacgca
     2761 aaggtatcaa tttttttaac tatcactatt taaatgccgt gtacctgcca aacaggtgca
     2821 ggttatgccc tgactattat ggcaactatt cagatatcag ctttggcgat tcatggcttt
     2881 tacgactttt agggcgaggc atgaatgaaa aaggtatacc caaaggttgg tcaagcatca
     2941 ttgcaaggtc acagaaggga ctttctcttt tagaggcagc tcaaaaggct catgctctct
     3001 atttagaaga aatagagcct ccagaaattc atgaatcatt cccatttaat atctcatata
     3061 agctcaatgg aatttttgta aggttgcagt ttgccaaaaa gaaacctgaa ttcattgggc
     3121 ttgcaaaacc agaaaaactt gatcgatatc tgtatcattt tatatataat attatattga
     3181 ttcttggttc aaccagtgtt tttaaattct ttttaagtat tatgccttta aattttaatc
     3241 tttttttaat aaaaaatttt aaaaagttaa tgggatataa acccaatgcc agggacaggg
     3301 ttttgcagta taatgaaaag tcaaatgttt aacaatttta taatggacat ttcaaaaaat
     3361 atttttatat ttttgtgtgc gatagttttc ttaaaaacaa aagctacttg ataaggaata
     3421 attatgagtg agcaatatta ctttaccctt aaaggattct ggaaacgagt caaacaggaa
     3481 tatgactatt acaccaggcg gccatggact ttaaaacagg ttggagaatt ttgggatact
     3541 gtcaatgatt atgatgctgt caatgaacaa ctgtatacgt atcatagaag atttaccaat
     3601 tcttatgagc ttgcatataa acatctaaaa aaagatacat acaatatgat agatatccag
     3661 gctcgaagtg gcaaaggcag tttattctgg catgaaaagg ggaaggtcac aagctctgta
     3721 tgtgttgatt tttctgattt tttaatttct cttgctgata aacgattgaa caaaacagga
     3781 ttacaatatt ctattgttaa agttcttaat tttccattac cgtttaatga tggacaattt
     3841 gatctggttt gttcgtatga gactattgaa catatttatg attactctgc cttttttgat
     3901 gaacttgtgc gaatcatgag taaagatgga ataatgattt tgacctgccc caatcgtatg
     3961 tgggaatggg ttcattggct ttcagcagca atcaatataa atcatagtga aggtccacat
     4021 cgttttttga aaagaggcga gttgattcaa tgcataaaga aaaatcgcat ggaaattctt
     4081 gaagaaaaca gtactattat ccttcccttc aataataaac tatccatagc aatcaataca
     4141 tttttagaaa actatctccc agagttcatt aaaagaatta ttgccttgcg aaggactttt
     4201 attcttggta agggagataa atagcaataa cattgaaaaa gtttacacta tatttactgg
     4261 tttgttttct tgtgtatttt gcaatctttg tgataataga tttttatgct gaaaaaaagt
     4321 atgatatact atacaatact atcaatactc catttgatgc tgctgtagtt ttttttagtg
     4381 attttgaaac tcataataca atcgatgatg aatcattgag gcgcttatct tttgcaattg
     4441 aattatacaa acaacatgca attaacaata taattttctg tggcggatgg cggccttcaa
     4501 taaatacttc cggttctaaa ttaatggcta caatggcaga ggaaatggga gttgacagaa
     4561 gtcatattta ttatgaaaca cattcaaggg atacagtgca aaattggaaa gaagctgaga
     4621 aaattatagt tgttaaaaat tttaaaaaaa taattttatt atcttctcca ttacatctga
     4681 tgagaataga aaaaatagtt actatccatg atgatattga tgttacatat agttcataca
     4741 gcaaaagaag cattattcca ccaaaaggat atttagataa tttttgtgat tataattata
     4801 atatggtatc gattatttca tattatttgt tgccttcaga attgtatcaa tatattgtga
     4861 ccaatttgcg aaattaaaat attaatactt tattaaaaat actgaacatg aataggtaaa
     4921 tgaattgagt aaaaaaatta attatcttaa tggaatagga ataatccttt tagcaatcat
     4981 tttgtggaaa atagatttta agcaagtttt taagtattta aaagatgcaa attactggta
     5041 tttgcttctt gcttatccat ttattttaat gaacattttt cttaaagctg tacgatggaa
     5101 ttttttaatg aaaacgcaag gtgtacgtaa ttccatattt gaatcctttt atgtatatct
     5161 atgggcattt tattttggtg ctgttactcc gggaaggata ggtgaaatat cgaaagcact
     5221 ttattatcag gaaaagtttg acaattttgg gagttcgttg tccccggttg ttgttgatag
     5281 gttgtatgat gtaggcattc gtattgcaat gatttttata ttatatcctt tttatagcca
     5341 tctctttcat ttaaaatatg ttgattttat tattttacta tcctgcatta tagctggtat
     5401 tgtggtatta gcccgatata aatcaattca aaatattatc atcacattta gtaaattttt
     5461 agttccaaaa aaatactatg caactatcga aaataatatt tcaggcttta taaatgatat
     5521 tattaagatg gtaacaacag taaaatatct tgcaatttgt atagttataa caataccaag
     5581 ttttttatgt tattgtatgg ttggatattt tatattgaaa agttttggga taaatatgac
     5641 atttacatac aatatatttt gccttgctgt ttccagtttt tctgtagcgg tgccaatatc
     5701 tgtttcaggt ttgggtgtta gagaagctgt aatgattttt ctttttaata gcattggtct
     5761 gggcagggaa agtgcagtgt tgttttcact atcaatcttt gcattaagcc ctatccttgg
     5821 atttcatggc tggcttgtaa atcttattat gctgattaga aatcatatca agaaaaggat
     5881 gaaagtaaaa gtaaactaag tataccatta catttacatg ataaagaatc atactttgta
     5941 ataattatct tgacatggaa aaagataaca attcattttt tataattaaa ttaatacata
     6001 ctatgtaaga gaggattcaa tgaaatcagg attaacaatt atagttccag tgtataatga
     6061 gattagtgca atagaacatt ccattaatca tttaaaggaa ataaaaaaga aatgcaaaaa
     6121 tattgatatg gagattatca ttgttaatga cggctctaca gatggaaccg aaaaaatatt
     6181 agcaaggatg cctaacaata aggatataaa agtaattaat cattctgaaa atcaaggtta
     6241 tggtgctgca ttaaagacgg gaatacgtgc ttcaaaacat caatacgtag ctattactga
     6301 tgcggatgga acatatccag atgaacgcat tcctgaattt tttgaaattg tcatcaatga
     6361 aggattggac atgttagtag gagcaagaat aggcgagtca gttcacattc catttattcg
     6421 aaaattaccc aaatgggtta ttaataaaat ggcaaattat atggttggga ctaaaatacc
     6481 tgatttaaat tcgggtatgc ggataatgaa aaaggatgtt gtaaaaaaat attttaagat
     6541 t
//
LOCUS       DAOSOX010000130         6508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11939_length_6508_cov_10.014567, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000130 DAOSOX010000000
VERSION     DAOSOX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11939_length_6508_cov_10.014567"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PLX22_09135"
     CDS             <1..1482
                     /locus_tag="PLX22_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20099.1"
                     /translation="VSQRLFDLQSALRQRLYKILTDIVDDQSIDNPVIFNYCVVLYNA
                     LESVCLLNKHYMELKWTGPEEYKQLLSPYIESLLKLSLLDTGTKEAIIQLIDQKMRES
                     EDLQKKPENINKKNLSIEQYIYDFVTTIRCFLYPAITNDCSLIAILKKETSLPSFESF
                     CVACVEALITGKPVALSDIINMFYVSAPKVSDTAWNFDHDELVKAGKDAESLKRKAKE
                     VLHKKLNPYDELFRFLTYAPEGKEVMIQVFDDQRKIVDKRNELYEDTYSKDIVMLLEG
                     MLLGFINEYGSMLDGSMLYFENGNNQYEGCIFTESYFMHEFARFAEILSEINIFRTNN
                     PTFHVTRDELIEGINKKKKSLYPIERFMTVIGDMFYDMGIELFMLYNRHRNWILNGAP
                     GSNDVIMRTAFSARTSGVLPFDDDEATPIPFSDCLISGFTKSRAMSKLLLHKPVIHAT
                     LRDGVFPQMTAFCLQVAHLCLNDRLYGHLDERTRILTQIRGLA"
     assembly_gap    1496..1595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1596..2530
                     /locus_tag="PLX22_09140"
     CDS             <1596..2530
                     /locus_tag="PLX22_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20100.1"
                     /translation="GVSLKDDYAGIDYKRLNALLQDFVNNGGRLYAAGYSYQFVEAMG
                     CSLSFFNNFPHFGTGGQIHCFLNDDFASFVMRNDIQLPVSYNGWVALENADAVILAKG
                     YFPTVQGEREGPLAFYKQYGKGRVYYSVYHSEIDSLYMRYFIFRVAYGALLDEMFHHA
                     WYWGQKVVFSVVDTFTSNEYARSYRCTLPRGTATIYLPSDCSLQVDIVAEKTIIGSWD
                     SGQQKQITFYNATPREVIVSVYQAARIPYQPFSLVIAKGKRMVPYNPVVKVSAGALAI
                     VMVIGLTWKRLNPRRFTGKRRYWFRDGHILNKQK"
     gene            2802..3563
                     /locus_tag="PLX22_09145"
     CDS             2802..3563
                     /locus_tag="PLX22_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20101.1"
                     /translation="MGKIISISNQKGGVGKTTTAVNLAAFLAEKGKRVLIVDIDPQAN
                     AGFGLGVNVEEMETTVYEVLIGQLPVEDAIYKTTIDNLHIVPSNMNLAGAQVELMDMD
                     DKEYLLKRALQKVRDTFDFILIDCPPSLGVLTLNSLVASDSVIIPLQCEYYALEGLSQ
                     LLKIIAMVQDTLNPELEIEGVLLTMYDPRTNLSQQVVADVKQFFQNKVFAAIIPRNVR
                     LSEAPSFGKPINLYDRSSSGSVSYEQLAMELLRSR"
     gene            3673..4542
                     /locus_tag="PLX22_09150"
     CDS             3673..4542
                     /locus_tag="PLX22_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT20102.1"
                     /translation="MAKKVLGKGLGAIITTSPTPASDFEKVLLQDKDRIVDIPVASIM
                     PNPEQPRVHFDQAEIAGLAQSIQHVGLLQPIIVRKQGDEYFIVAGERRWRACKLLGKP
                     TIRAIVMNTSEEQNLTLALIENIQRTNLDPIEEAKAYKILSTRFKLKQSEISDRVGKD
                     RATVANILRLLNLPEHMQEALQDGKINVGHAKLLLSVSMEEQEALYEKIINQGLSVRD
                     VERAIADVSKSSIKKKKKAKDAHLRKLEEELISFLGTRVAIHHSAGKGRIEISFYSLE
                     DFDRIVSIIKGER"
     gene            complement(4543..>4800)
                     /locus_tag="PLX22_09155"
     CDS             complement(4543..>4800)
                     /locus_tag="PLX22_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT20103.1"
                     /translation="YACEFTVEGKHFMVFHGNNVPMFREAVNSGKYDYIIKGHTHIFE
                     NYLSGKTRIINPGALYRGEELTVAILDTATDKVEKIRIDIE"
     assembly_gap    4801..4900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4901..>6508)
                     /gene="metG"
                     /locus_tag="PLX22_09160"
     CDS             complement(<4901..>6508)
                     /gene="metG"
                     /locus_tag="PLX22_09160"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOT20104.1"
                     /translation="GTDEHGDKIVKAALHNNTQPQEYTDKISAIFKEQWKSMGIEFND
                     FIRTTEERHKKVVQHVLQKVYDNGDIYFGSYGGYYCFGCERYFTDKEMVDGKCPDHQK
                     KLDYIQEENYFFKMSKYQQWLIDYIHEHPDFIRPERYKNEVLAMLEGEALEDLCISRP
                     KARLSWGIPLPFDTNFVTYVWFDALINYISAIGYPDSDLFKKFWPVSNHLIAKDIVKP
                     HGIFWPTMLKAAGIEPYQHLNVHGYWNMESTKMSKSLGNVVTPSQLLEKYGNDQIRYF
                     FLREMTFGLDAKFSEEAITNRINYDLANDLGNLINRTFNMVQKYCDAKVPPFHSDEPA
                     NRDDMQQALQVSMHQYIDFVHQFQFNIALERLWDFVRYCNKYIDESKPWQVAKENNVK
                     KLQSILRNLLEGIHAVAILLSPVCITKSSLVIKALKSSAQCTIDTLPAMSSLSDGTTL
                     DSPGILFPRLEKETEAVNSAHSTKKEAFMETTPEGLIDIKDFLKVELRIAKVLQAKAV
                     EGSSKLIELSIDTGKDIRTIIAGLAPHY"
BASE COUNT         1853 a         1140 c         1401 g         1914 t
ORIGIN      
        1 gtaagccagc ggttatttga tctgcaatct gcactgcgcc agcgtctgta caaaatttta
       61 acagatattg tagatgatca gtcaatcgac aatcctgtta tattcaatta ctgtgtggtg
      121 ctgtacaatg ctttagaatc agtgtgtttg ctgaataaac actatatgga attgaagtgg
      181 acaggacctg aagaatataa acagttacta tcgccctata ttgagtcgtt gcttaagctt
      241 tcgcttcttg atacaggaac aaaggaagca atcatccagc ttattgatca gaaaatgcgt
      301 gagagtgaag atttgcaaaa aaaaccggaa aatataaata aaaagaattt atctattgaa
      361 cagtacatct atgattttgt taccaccata cgctgttttt tatatccggc aattaccaat
      421 gactgttcat tgattgcaat tttaaaaaag gaaacgtcat tgccctcatt tgaatcgttt
      481 tgtgttgcat gcgttgaagc tcttataacc ggtaagcctg ttgcactttc tgatataatc
      541 aacatgtttt atgtaagcgc tcccaaggtt tcggatactg catggaattt tgatcatgat
      601 gagcttgtaa aagcaggtaa agatgcggaa tcgttaaaga gaaaagcaaa agaggtttta
      661 cataaaaagt taaatcccta tgatgagctt ttcaggtttt tgacgtacgc ccctgaaggc
      721 aaagaggtta tgattcaggt ttttgatgac cagcgtaaga tagttgataa acgaaatgag
      781 ctgtatgaag atacatacag caaagatatt gtgatgctgc tggaagggat gctgcttgga
      841 tttataaatg aatatggcag tatgcttgat ggtagcatgc tctattttga aaatggtaat
      901 aatcagtatg aaggatgcat ttttaccgag tcatatttta tgcacgagtt tgcacgcttt
      961 gcagagattc tttccgagat aaatatattc agaactaaca accctacatt ccatgttacc
     1021 cgtgatgaat taattgaagg gataaacaaa aagaaaaagt cattataccc cattgaacgg
     1081 tttatgactg tgattggtga tatgttttat gatatgggga ttgaattgtt tatgctgtat
     1141 aaccgtcacc gcaactggat attgaacgga gcgccgggga gcaatgatgt aatcatgcga
     1201 acagcatttt ctgcacgaac ttcaggcgtg ttaccctttg acgatgatga agcaacccct
     1261 ataccatttt ctgattgttt gatcagtgga tttacaaaat caagggctat gtcaaaatta
     1321 ttactgcaca aacctgtcat tcatgctaca ctgcgcgatg gtgtattccc acagatgaca
     1381 gccttttgtt tgcaggttgc tcacctttgt ttaaatgaca ggctctatgg tcatttagat
     1441 gagcgaacaa ggattttaac gcagatacgg gggcttgcat gaggatagcg ctgttnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggga gtgtcattaa aagatgatta
     1621 tgcgggtatt gactataaac gattaaacgc tttattgcag gattttgtca acaatggtgg
     1681 acgactgtat gcagcaggat attcgtatca gtttgttgaa gcaatgggat gctcgctgtc
     1741 attttttaac aacttccccc attttggtac aggcggtcag atccattgtt ttttgaatga
     1801 tgattttgct tcctttgtta tgagaaatga tatacagctt ccggtcagct ataatggatg
     1861 ggttgcatta gaaaatgctg atgctgtgat actggcaaaa ggttatttcc ccactgtgca
     1921 gggcgagcgt gaaggaccgt tagccttcta caaacagtat gggaaagggc gggtatatta
     1981 ctcggtatat cattcggaga tagacagcct gtatatgcgc tactttattt tcagggtggc
     2041 gtatggtgct ttgcttgatg aaatgtttca ccatgcatgg tactgggggc aaaaggtggt
     2101 gttttcagtt gtcgatacgt ttacatcaaa tgaatatgcc aggagctatc ggtgtacatt
     2161 gccacgagga actgctacca tttatctccc ttctgactgc tccttacagg tggatattgt
     2221 tgcagaaaaa acgataatag gttcatggga tagcggacag caaaaacaaa tcacgtttta
     2281 caatgcaacc ccgcgtgagg tcattgtcag tgtatatcag gcggcacgaa ttccttacca
     2341 gccattttcg cttgttatag ctaaaggcaa acgtatggtg ccctacaatc cggtggttaa
     2401 ggtaagtgca ggtgctttag cgatagttat ggtaattggt cttacctgga agagattaaa
     2461 tccacgtcgt tttaccggta agcgcagata ctggtttaga gatggtcata tcctgaataa
     2521 acagaaataa tattttttgt agctgaggaa aaagcttgca ttctgaaatg acggatgtgt
     2581 acatttgtat agactttttg aaattatagt atcatagatt tatggtaggg atgcggattc
     2641 caaatccggt ataaaataac attgacggat gtatatataa ggaataagtc acagtgcgat
     2701 gttcactgtg tggatgcaat ctgacaatat gaaaggtatg ttttgataca ggtgtttagc
     2761 ctgttaatgg aataatagaa ttaattataa gggagcttgc catgggtaag attatttcaa
     2821 tatccaacca aaaaggggga gttgggaaaa ccacgacagc agtaaattta gctgcctttt
     2881 tagcggaaaa gggaaagcgt gtgctgattg tggatattga tccccaggct aatgcaggtt
     2941 ttgggctggg cgtcaatgtt gaggagatgg aaaccactgt gtatgaagtc ctgataggtc
     3001 agcttcccgt cgaagatgct atttataaaa caactatcga caatttacat attgtaccct
     3061 caaatatgaa tctggcaggt gcacaggtag agcttatgga catggatgac aaggagtacc
     3121 tgctgaagcg tgcgcttcaa aaggttcggg atacctttga ttttattctt attgattgcc
     3181 ctccgtcact gggtgtttta acgttaaata gccttgttgc ttctgattcg gttattattc
     3241 cactacagtg cgaatactat gcgcttgaag gtttgagcca gttgcttaag attatagcaa
     3301 tggtgcagga cacccttaat ccagagctgg aaattgaagg tgtgctgctt accatgtatg
     3361 acccgcgcac caacctttcg caacaggttg ttgctgatgt taaacaattt ttccagaaca
     3421 aagtttttgc tgcaataata ccacggaatg tacggctttc tgaagcgcct tcatttggca
     3481 agccaataaa tttatatgac aggtcatcaa gcggcagtgt aagctatgaa caattagcaa
     3541 tggagctttt acgctcacgg tgacctgttg ccatgaccgg ttacattttg tactactggt
     3601 gatggtgcag ctctactgaa agctggcaat gataatgtat gtcaacattt attatgaatg
     3661 aggatgtgaa caatggctaa aaaagtattg ggcaagggtt tgggtgcaat tattaccact
     3721 tcgccaacac cggcgagcga ttttgaaaag gtattgttgc aggataaaga ccgcattgta
     3781 gatataccgg ttgcatcaat aatgccaaat ccagaacagc cgcgtgtgca ttttgatcag
     3841 gcagagattg cagggcttgc acagtccatt cagcatgttg ggctgctgca gccaatcatt
     3901 gtgcgcaaac agggcgatga gtatttcatc gttgctggtg aacgcaggtg gcgtgcatgt
     3961 aaacttctgg ggaagcccac catcagagct attgtcatga atacctccga agaacaaaac
     4021 cttaccctgg cgcttataga aaatatacag cgcaccaatt tagaccccat tgaagaagca
     4081 aaagcatata agatactgtc cacacggttt aaattaaaac aatcagaaat ttctgacagg
     4141 gttgggaaag accgtgcaac agttgccaat attttacgcc tgcttaatct tcccgaacat
     4201 atgcaggaag cgcttcagga tggaaaaata aatgttggac atgcaaagtt gctgttaagc
     4261 gtatccatgg aagagcagga agcgctttat gaaaaaatta ttaatcaggg gctgtcggta
     4321 cgtgatgttg agagagctat cgccgatgtt tcaaaaagta gtatcaaaaa aaagaagaag
     4381 gcaaaggatg cacatctgcg gaaattggaa gaggagctta tttctttttt agggacgcgt
     4441 gttgcaatac accatagcgc cgggaagggc agaatagaga taagctttta ctcattagaa
     4501 gattttgata ggattgtaag tatcataaaa ggcgagcggt aactattcaa tgtcgatgcg
     4561 gattttttca accttatctg ttgcagtatc aagtatggcg acggtaagct cttcaccgcg
     4621 atatagtgcg ccgggattta taatgcgagt ttttcccgaa aggtaatttt caaagatatg
     4681 ggtatgccct ttaataatgt aatcatattt accagagttg actgcctctc tgaacatggg
     4741 aacattgttg ccatgaaata ccatgaaatg ttttccctct acagtaaatt cgcatgcata
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtagtgtggt gcaagcccgg
     4921 ctatgatggt tcgaatatct ttaccggtat ctattgacag ttcaataagt tttgatgaac
     4981 cttccacagc ctttgcctgt aaaactttag caattcgcaa ttcaaccttc aaaaagtctt
     5041 tgatatcaat caagccttca ggagttgttt ccataaatgc ctcttttttt gtactgtgtg
     5101 cactgtttac agcttcagtt tctttttcta acctggggaa aagaatacct ggtgaatcta
     5161 aagtagtgcc atcggataat gatgacatgg caggtaaagt atctatggtg cattgtgctg
     5221 atgatttcag tgcctttatg actaacgaag attttgttat gcaaacagga gatagcagga
     5281 tggcaacagc atggatacct tctaaaagat tgcgcaatat tgattgtagt ttcttaacat
     5341 tattttcctt tgcaacctgc catggcttac tctcgtcaat atatttattg cagtacctga
     5401 caaaatccca taatcgttct aatgctatat taaactggaa ttgatgcaca aagtcaatgt
     5461 attgatgcat agacacctgt aatgcttgtt gcatgtcatc gcggttagca ggttcatcac
     5521 tatgaaaggg aggcacttta gcatcacaat acttttgaac catattgaag gttcggttaa
     5581 tgagattccc taaatcattg gcaagatcat agttaatcct gttggtgata gcttcttcag
     5641 aaaattttgc atcaagacca aaggtcatct cgcgtaaaaa gaaatatcgt atctgatcat
     5701 tgccatattt ttccagaagc tgtgatggtg ttacaacatt ccctaatgat tttgacatct
     5761 ttgtgctttc catattccag tagccgtgta cattgagatg ctgatatggt tcaataccag
     5821 cagcttttag cattgttggc cagaaaattc catgaggctt tacgatatct tttgcaatga
     5881 ggtgatttga aaccggccag aattttttaa aaagatcact gtcaggataa ccaatggcgc
     5941 tgatgtaatt tattaatgca tcaaaccata cataggtaac aaaattggta tcaaagggaa
     6001 gcggtatacc ccatgaaaga cgcgctttag gacgcgatat gcacaaatct tccagtgctt
     6061 cgccttcaag catggcaagc acttcgtttt tatagcgttc agggcgtata aaatcaggat
     6121 gttcatggat atagtcaata agccactgct gatatttgct cattttaaag aaataatttt
     6181 cttcctggat atagtcaagc ttcttctgat gatcggggca tttgccatca accatctctt
     6241 tgtcggtaaa gtagcgttcg cagccaaagc agtaataccc tccatagctt ccaaagtaga
     6301 tatcaccatt atcatacacc ttttgtaaaa catgctgtac tactttttta tggcgctctt
     6361 cagtagtacg gataaaatcg ttaaactcaa tccccatgga tttccactgc tctttaaata
     6421 tggcgcttat tttatcagta tattcctgag gctgggtatt gttgtgcagt gcagccttaa
     6481 caattttatc accgtgttca tccgttcc
//
LOCUS       DAOSOX010000131         6506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11944_length_6506_cov_9.132228, whole genome shotgun sequence.
ACCESSION   DAOSOX010000131 DAOSOX010000000
VERSION     DAOSOX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11944_length_6506_cov_9.132228"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLX22_09165"
     CDS             complement(<1..295)
                     /locus_tag="PLX22_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20105.1"
                     /translation="MLKRIIKVSIMGPNYRCYCIIICAIALLIAQCSLLNSASNKVVQ
                     LYHNVEINWTDQLITAKASSMPSITDDGTPVDRNTLETISINYARSRSIDEARD"
     gene            complement(340..2013)
                     /locus_tag="PLX22_09170"
     CDS             complement(340..2013)
                     /locus_tag="PLX22_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOT20106.1"
                     /translation="MLQYQITTAKSGSTSLQLVDGDKVIRLHSAYDPLKEAIRAAEEF
                     NPGRAGIIVVCGLALGYHIIALHQRFPDHTILVIEKDNELVSLVKKENPLVLSLCSIV
                     HNEEEIAAIIETWDIRSFRGTALFIHRPSYMLKREFYDSMVATLHKQISSRISDLLTR
                     FEFEALWIKNILLNSVQMHTAYPVQSLFGKFKGIPGIIVSAGPSLIHSLEALRQAYDK
                     ALIVCVDTAYKVLQRHGIQPHIVMTLDAQTHSIKHFLGSSTEPLLLADVVSCPKVVRL
                     FNKKMFSTTAKYYNTPNGKIEKESTPIMDWVQHYTGYIGDVQSGGSVATSAFDLLLNA
                     GCSPIVLAGQDLAYTGREIHCRGTHHNDDWLSLTNRYNNLDTINQNIIRRRKIKYVPS
                     NDGTTVITDFVLDLYRSWFEDSAKKVGVPVYNTGAGAVIANTQFATLQSLVLQWQKAF
                     PAPEDIIYKQLSNKKYIDTSKLMKQLAKVKDALHNLDKAVQAGNTSCIYSILDDDAID
                     TLISPFMRKTLFYIARHNLEQEKIDTLIKDAALSAAHLLLKLFDRIAIK"
     gene            complement(2022..3488)
                     /locus_tag="PLX22_09175"
     CDS             complement(2022..3488)
                     /locus_tag="PLX22_09175"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOT20107.1"
                     /translation="MNIPQLIAHFDKNEPVSISFYYPNEAISRTLNAMLAAILSKNDQ
                     IYLLDMMITILREIIVNATKANAKRIYFKKLNLDINDPQSYKTGIEHFKKDIVGQLDT
                     LENELKNSNLKVTLSICKEDKGVTFIITNPAAIHSEELNRIKTRIEKAKEYLDFTQAY
                     EDVYDDTEGAGLGIVLVILLLKNAGIDPSNYNIGTDGKVTRVVLSLPLQLRPEAITTK
                     IKQQIINAIQGIPTFPEHILVLLQLCRNKEVEIKDITMTLLHDPALSTDVLKLANSAG
                     FVPSKRVNNLSEAVMTIGLKNLESLLITISARKILDDRFQKFEQIWEHCNRVASYART
                     IASKYKMNAIIEQVSLCGLLHDLGKIVLLSVDLDVTQWIAKLVSDRRIRTSTVMEEIS
                     IGISHSGIGELIASKWQLPEYLIEAIRHHHTPLSTDSRFKDIVYITYLANLLCGVESR
                     RYFYYYCDDEVSRYFTLESQSDFENLGKLCQDIYNKLK"
     gene            complement(3693..5618)
                     /locus_tag="PLX22_09180"
     CDS             complement(3693..5618)
                     /locus_tag="PLX22_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOT20108.1"
                     /translation="MLCIKWEDYNKYIAGWGIPSIAASSCACSSILHTYQYILSVSYY
                     PFISIIDLVSFLFIFALIYTLLYSFNKNKIIALLKIAIAFIWIAAVIATASFRTIAMC
                     AYKIAPHKGIFHGKVLHTQHKRYNTIATIWCTGINGQYKAYCHFDHVHTIHNHDVIVF
                     NATAYPTRDDNNSKQYLAQRGISAVFYLSNNSIIMHTVAAKNYRTKVQELLQHSLYRT
                     MTHDAASLLAALYLGNKEIIHPHVQMAFKEAGVLHVLAASGLHVGIIMATILALCRLC
                     KAPKKMSVMIASSGVLIYLYISYQPVSLLRASIMCAVAATAVLLNMQKHSINLLCCAA
                     CIILCIYPYEIFSAGFQLSFMATAGILLVYKQYHNGLKHCGRITSALAITFAAQLFTM
                     PVSVYHFHELSVTAFLANLVIIPMVSIFMIAGLCIPLFCHAGLTNVASFIMHHMYTII
                     VKLTQLFASFHGTIYIDSLLLAGIYLIIVIIPLVYKKKANIVILCGIIALLLYHNQPQ
                     NKVIHYNGGDSSWYTVLLDKGSACCIIDLKSFNDYGNLTSQLKKHSVTKVHIMLPNAS
                     NKNCTYAAMLAKQFALQDIAVGDYALYAIKKITTIASVDAIPIYFTIPHNKTMMDRDA
                     LIQLYYTTKNHTMKGQK"
     gene            complement(5590..6177)
                     /locus_tag="PLX22_09185"
     CDS             complement(5590..6177)
                     /locus_tag="PLX22_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20109.1"
                     /translation="MKQYCYKTGFLVIFLFFLCSTNNIVYAQELFFTYIGATIIGGTN
                     SISYEGWVDNKQSTVDSNGAVYGGGLIACIFVRQFAGEFSLTYMQYTSDSKPDVAVSH
                     PVAAVKGKYFYPLHAVFQPGIAVGLYLDLPPASSSYDGGAGGNIAICSAFTLSSEIKM
                     LIDIYAQYGSFGMGESSTKKESGIALSVVYKMGRL"
     gene            complement(6195..>6506)
                     /locus_tag="PLX22_09190"
     CDS             complement(6195..>6506)
                     /locus_tag="PLX22_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20110.1"
                     /translation="QDVGDGYSGGIASIVSGYNALLAAQGTGGYTIKLRNFEKAYPEI
                     VIVADSNFLLKKLILKDKQGDSTTYTISNLIAGENIPNGYFDFKPPTSAQSVKNPLNI
                     Q"
BASE COUNT         1939 a         1193 c         1279 g         2095 t
ORIGIN      
        1 tatctctggc ttcatctatt gaacggcttc gggcatagtt tatggaaata gtttctaagg
       61 tgttcctatc aactggtgtg ccatcatcgg taatagatgg catactggaa gcctttgcag
      121 taatgagctg gtcagtccag ttgatttcaa cgttgtgata aagctgaaca accttattgc
      181 tggcagagtt caataaagaa cattgtgcaa ttaaaagagc tatcgcacaa ataataatac
      241 agtaacatcg gtagtttggt cccataatgg atactttgat tattcgtttt aacatttatc
      301 gtcatgccgg tggtcaaaat atagctaaat tattcaatat cattttatgg caatcctgtc
      361 aaatagtttt aacagcaggt gagcagccga taacgctgca tccttgataa gtgtatcaat
      421 cttttcttgc tcaagattgt ggcgtgcaat gtagaacaat gttttacgca taaatggtga
      481 aatcagtgta tcaatagcat catcatccag aatgctatag atgcacgaag tattgcccgc
      541 ttgtaccgcc ttgtcaagat tgtgtaatgc atctttgact tttgctaatt gtttcataag
      601 tttggatgta tcaatatatt tcttgtttga tagttgttta taaatgatgt cttccggtgc
      661 cgggaaggct ttctgccatt gcaaaactaa tgactgcaac gttgcaaatt gcgtattggc
      721 aatgacagcg ccggcacctg tattataaac agggacacct accttttttg ctgaatcctc
      781 aaaccagctt cgatatagat ccagaacaaa atcggtgatt actgttgtgc catcatttga
      841 tggcacatat tttatcttac gtcttcggat aatgttctgg ttgatagtat caagattgtt
      901 atatcggtta gtgagcgata gccagtcatc attatgatga gtgccgcggc aatggatttc
      961 tcttcctgta taggcaaggt cctggcctgc aagaacaatt ggagagcatc cggcattcag
     1021 taaaaggtca aatgcgcttg ttgcaaccga acctcctgat tgcacgtcac caatataacc
     1081 ggtataatgt tgcacccaat ccattatagg tgttgattct ttttctattt ttccatttgg
     1141 tgtgttatag tattttgcag tagtgctgaa cattttttta ttgaaaagcc tcactacttt
     1201 tgggcagctt acaacatctg caagcagcaa tggttcagta ctgcttccaa gaaaatgttt
     1261 tatgctgtgt gtctgggcat ccagtgtcat aacaatgtgc ggttgtatac catgacgttg
     1321 cagtacttta taggcggtat caacgcatac aattaatgct ttatcgtacg cctgccgtag
     1381 tgcctccagt gaatgtatca acgatggccc ggctgaaaca atgatgccgg gtataccttt
     1441 aaatttgcca aacaacgatt gtaccggata agcagtatgc atctgaacac tgttgagcaa
     1501 aatgttttta atccatagtg cttcaaattc aaatcgtgtt aatagatcgc tgatacgcga
     1561 gcttatctgt ttatgcaaag ttgctaccat tgaatcataa aattcccttt tcagcatata
     1621 cgatggacgg tgtatgaata aagctgtgcc tctgaatgaa cgtatatccc atgtttcaat
     1681 aattgcagca atttcttctt cattatgaac aattgaacat agtgataaaa ccagaggatt
     1741 ctctttcttt accagggaaa caagctcatt atctttttct attacaagaa tggtgtggtc
     1801 agggaaacgt tggtgtaatg ctatgatatg gtagcctaac gccaatccgc atacaacaat
     1861 tatgcctgct ctcccgggat taaattcttc agcagcacgt attgcttctt ttaatggatc
     1921 atacgctgaa tggagacgta tgactttatc gccatcaact aactgtaatg atgtgctgcc
     1981 gctttttgct gtagtaatct ggtactgtag catcgtgcat attattttag tttattatat
     2041 atatcctggc atagtttgcc cagattttca aaatcagatt gtgattcaag cgtaaagtat
     2101 cgggatactt catcatcaca gtaataataa aagtaacgac gtgattccac cccacacaaa
     2161 agattagcta aataggtaat atacactatg tccttaaacc tgctatcggt tgaaagtggt
     2221 gtatgatgat gccgaatagc ctcaatgagg tattctggca gttgccattt tgaagcgatg
     2281 agctcgccta taccggaatg gcttatccca attgatattt cctccatgac agttgatgtc
     2341 cgtattctgc ggtcagatac cagtttagct atccattgtg ttacatccaa atcaactgaa
     2401 agtaaaacaa ttttacctaa atcatggagc aagccgcata atgagacctg ctcaataata
     2461 gcattcattt tatattttga ggcaatagtc ctggcatagg atgctactcg gttgcaatgt
     2521 tcccagattt gttcaaactt ctggaagcgg tcatccagta tcttacgagc gcttattgtg
     2581 atgagcaatg attctaaatt ttttagtccg atagtcatta ccgcttcgct tagattgttt
     2641 acccgtttgc ttgggacaaa accagccgaa ttggctaatt ttaacacgtc agtggataac
     2701 gctggatcat gcagaagggt catggtaata tcttttattt ctacttcttt gtttcggcaa
     2761 agctgtagca gtaccagtat atgttcagga aatgttggta taccttgtat ggcatttata
     2821 atctgttgtt ttatttttgt agtaattgct tcgggacgca gttgcaatgg cagtgatagt
     2881 acaacccgtg taaccttgcc atcagtgcca atgttataat tggatggatc tatgccggca
     2941 ttttttaaaa gtaagataac aagaacaatg ccaagccctg caccttcggt atcatcataa
     3001 acatcctcat acgcctgagt aaaatccaga tactcctttg ccttttcaat acgggttttg
     3061 atacggttta attcttcaga atggattgct gcgggattgg taatgataaa ggtaacgcct
     3121 ttatcttctt tacagatgga cagggtaact ttaagattgc tgttttttaa ctcattttcc
     3181 agcgtatcta attggccaac aatatctttt ttaaaatgtt caatgccagt tttgtaactt
     3241 tgcgggtcgt taatatccag attaagtttt ttgaaataaa tacgctttgc atttgctttt
     3301 gttgcattga caataatctc gcgtaaaata gttatcatca tgtctaaaag gtaaatttga
     3361 tcatttttgg ataggatggc agccagcatc gcgttgaggg ttctggaaat agcttcattg
     3421 ggatagtaaa atgaaatgct aacaggctca tttttatcaa aatgagcaat aagctggggt
     3481 atattcactg gcatcatggt attttttact atttttaatt aaagtaatat tacataaaat
     3541 ctatgacttg caatactact aatatcggat aaagtaaatg tatataataa ataaaggact
     3601 atcaagtaat ttatttttta taaaatatca ataagtgcta ttatttccaa acaaatgttt
     3661 agcctttttc aatataatgt atactgcaat ggctactttt gtcccttcat tgtatggttt
     3721 tttgttgtat agtatagttg tatcaatgca tctctgtcca tcatagtttt attatgaggg
     3781 atagtaaaat aaattggtat ggcatcaacc gatgctatcg tggttatttt ttttatagca
     3841 tagagcgcat aatccccaac tgcgatgtcc tgtaatgcaa attgtttggc aagcatggct
     3901 gcatacgtgc aatttttgtt tgatgcattg ggaagcatga tgtgcacttt tgtaactgaa
     3961 tgttttttta attgcgatgt caggttgcca taatcgttaa aacttttcag gtcaattata
     4021 cagcaggcgc ttcctttatc aagcagcact gtataccagg aactatcgcc accattataa
     4081 tgtattacct tgttttgtgg ttggttgtga tacagaagca atgcgatgat gccgcacagt
     4141 ataacgatat tagccttttt tttatatacc agagggatta ttacaataat caggtatatg
     4201 cctgcaagca gtaatgaatc aatatagatg gtgccatgga atgaagcaaa caactgtgta
     4261 agctttacaa taatggtata catatgatgc attatgaatg atgcaacgtt agtcaatcct
     4321 gcatggcaaa aaagcgggat gcataatcct gcaatcataa atatacttac cattggtatg
     4381 ataacaagat tggctaaaaa tgctgtaacc gatagctcat ggaaatgata tacactcacc
     4441 ggcatggtaa acagctgtgc agcaaaggta attgcaagag ctgaagtaat acgtccgcaa
     4501 tgttttaatc cgttatgata ctgtttgtaa acaagcaaaa tgccggcagt tgccataaat
     4561 gaaagctgaa agccggcgct aaatatttca taagggtata tgcacagtat aatacaggct
     4621 gcacagcaca aaagatttat gctgtgtttt tgcatattca atagtactgc tgttgctgca
     4681 actgcgcaca tgatggatgc tcgcaacagc gataccggct gataggatat ataaagatat
     4741 atcagtacgc cagatgacgc tatcatcact gacatttttt tgggagcttt gcaaagcctg
     4801 catagcgcaa ggattgtagc cataataatg cctacgtgaa gtccgcttgc cgctaaaaca
     4861 tggaggacgc ctgcttcttt aaatgccatc tgtacatgcg gatggataat ttctttattg
     4921 cctaaataca gtgcagctaa cagtgatgcc gcatcatgcg tcatggtacg gtaaagagaa
     4981 tgttgaagta gttcctgcac ttttgtgcga tagtttttgg cagcaacggt atgcataata
     5041 attgaattgt tgctcagata aaatacggcg cttattcctc gctgtgcaag atactgtttg
     5101 ctgttgttat catcgcgtgt agggtaggca gtggcattaa atacaatgac atcatggtta
     5161 tgtatggtat ggacatgatc aaaatgacag tatgctttat actgtccatt tatgcctgtg
     5221 caccagatag tagcgattgt attgtaccgt ttatgctggg tatgcaaaac tttcccatga
     5281 aatatgcctt tgtgtggtgc aattttgtat gcacacattg ctatggtgcg gaacgatgcg
     5341 gtcgcgataa ctgcagcaat ccatataaag gcaattgcta ttttcaataa ggctattatt
     5401 ttatttttat taaatgaata aagtaatgtg tagattaagg caaaaatgaa taaaaaagac
     5461 actaagtcaa tgatagagat aaaaggataa tatgacactg acagtatata ctgataggta
     5521 tggagtattg aagaacaggc acatgatgac gctgcaatgg atggtattcc ccatcctgcg
     5581 atgtatttgt tataatcttc ccattttata cacaacactt aatgctattc cgctttcttt
     5641 ttttgttgat gattcgccca taccaaaact accatactgg gcataaatgt caatgagcat
     5701 ttttatttcg gatgataatg tgaaagcact gcaaatggca atatttccac ccgcaccgcc
     5761 atcatagctg gaagatgctg gaggcaaatc taaataaagt ccaactgcta tgccaggttg
     5821 aaacacagcg tgaagtggat aaaaatattt tcccttcaca gcagcaacag gatggcttac
     5881 tgcaacatca ggtttactat ccgatgtgta ctgcatgtag gttaatgaaa attcaccagc
     5941 aaactggcga acaaaaatac aggcaatcaa tccacctcca tacactgcac cgttactatc
     6001 aacagtagat tgtttgttgt caacccagcc ttcatatgaa attgagttgg tgcctcctat
     6061 aatggttgcg ccaatatagg taaaaaataa ttcctgagca tagactatat tgtttgtact
     6121 gcataaaaaa aataaaaaga ttaccaaaaa accagtttta taacaatatt gcttcaataa
     6181 tataccatcc attgtcactg tatatttaat ggatttttaa cagactgtgc acttgttggt
     6241 ggtttaaagt caaaatatcc attagggata ttttctccag caatcaaatt ggaaattgta
     6301 taggtggttg aatcgccctg tttatcttta agtataagct tcttaagtaa aaaattacta
     6361 tcagcaacaa tgactatttc cggataagct ttttcaaaat ttcgcaattt aatagtataa
     6421 ccgccagtac cctgcgcagc cagtaaagca ttatatcctg atacaattga tgcaatgcct
     6481 cctgaatacc cgtcgccaac atcctg
//
LOCUS       DAOSOX010000132         6502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_11955_length_6502_cov_9.252521, whole genome shotgun sequence.
ACCESSION   DAOSOX010000132 DAOSOX010000000
VERSION     DAOSOX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11955_length_6502_cov_9.252521"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..1415
                     /locus_tag="PLX22_09195"
     CDS             483..1415
                     /locus_tag="PLX22_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20111.1"
                     /translation="MATKLWPGVRARLVVYKINVPKEAQMSQKKSLFNKFSTIFIIFF
                     TLPIIISCGESKEMIRQRQENELREKQRQEALQKRRAEIDAVINDLKTQYQDPFDLSD
                     EKNCELFARAYNNPEENPKIRNSTDTSENEFQYQKDKIQYSQLRNKKYAIMSFNIFGN
                     ELQKYDFQKKSFSLQFKSYTIMAPEGNVIYTKKRPPVVKLLVKGVPDIDIKIEPDLAE
                     NFKSGITNTATGFGAMYGLESQYKDRAILYVIFKINGKILDDDIQHISVDIADYVCFW
                     YIDAGSSISQGGRYGNSQCSDIIECSSDIKIFRK"
     gene            1435..1722
                     /locus_tag="PLX22_09200"
     CDS             1435..1722
                     /locus_tag="PLX22_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20112.1"
                     /translation="MTKIEKLLKIGPYQLEVTGIDDGRSYIVNVFYKKYYIPPTYSYN
                     HTDFNNIVNSQESGLFYGKLDDVMSDFIRIIETDTMIENKIKQIDKYIESK"
     gene            2457..4163
                     /locus_tag="PLX22_09205"
     CDS             2457..4163
                     /locus_tag="PLX22_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT20113.1"
                     /translation="MKDPKKDQACIERYKELLPNLADYVAHYAKKKPQELAIIEHNTG
                     AKVTWKQFNTSVSAFAAKLLSIGLNKGDIIATSLPLLKEHVYLMYACYRSGIIIAPLD
                     LRLKTKEIQYCLDRMKPKAYFFLGKTPVADFRPIIAEVMKTSPYIKHWVQFQTEPEFI
                     MEGAIGITDFVKDIKTVFIKSLFTGSVRRARKKVQKRDASLIIFTTGSTGSPKPALLC
                     HEGILTQNIGLAIGFELRESDRFLVNLPPSHVGCTTEQLATTIYGGGTSVLLHIFDPK
                     LSLEAIQTHKITCIGQIPALYNMEWLLPNYKDYDLSSLRFAIYGGQAVSREFLVKMKS
                     MSPQIGTGLGLTETSGFCTYTDVNADVDEIVKSIGYDMPLCPLSIREPMNSDGSAGKE
                     KSKGEIGEICFSGPQIFLGYLGDPENTRKTISTDGICYTGDLGYYDEEGLHFSGRSKL
                     VIKPKGYQVYPDDVEHHISEKLKGKVNMVAIVGAEHNVYSEAIIAFVEPLPGVEVTPE
                     DVMKACEDISAYSRPSHVEIIKPGQFPLNRVAKTDYVVLKDEAKKIVEKLRSEGKWDK
                     AK"
     gene            4353..5228
                     /locus_tag="PLX22_09210"
     CDS             4353..5228
                     /locus_tag="PLX22_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20114.1"
                     /translation="MERTDIWYCTDNDKGKEIYETLKLLGLNIVLVEDKNFSSCNVLP
                     SQINIFIIDLVTVEFSEILSMLRKDERLHKFIKYLILFKKQIRKASQLSIDVMHLEIL
                     SRPIKKREFSLLLEKSIIVERYKEMLNLYSLEATERIEAYTNLLDINRKESFFDEKEK
                     NIFSKIINFEKHILKEQKELNVAIANYTRLRQKELFDLKKRIDAEEMLSELRRKELMD
                     AKSVIEAQSAVIEYSSKELQSAEEIIHATEAVQELSRLEAIKLHEELEKEKKKNEVLQ
                     KKIDMLEKKINNSKS"
     gene            5324..5779
                     /locus_tag="PLX22_09215"
     CDS             5324..5779
                     /locus_tag="PLX22_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20115.1"
                     /translation="MITMQNLFSKRDLITLYNEYVHPDIKSDVLTLIDAMKNEKTDDR
                     KIALRLGFANLNQYYSASHQDIINHFFLMMVSSRYTKPSDIKNSAYLRLAISLSLLFA
                     FLDNGMALVEKDIGFRCAKLTYNGNSFTMKIYFYYSNKKWLLTDGRHCL"
     gene            5828..6094
                     /locus_tag="PLX22_09220"
     CDS             5828..6094
                     /locus_tag="PLX22_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20116.1"
                     /translation="MEWQKILVDAVQDGKIKELHLKKIPVLKTCDNWREVEPIGWIDH
                     QMKLTHYKGALVRLRGNIYFVTEKTINALSEYINWKISQRIDVI"
     gene            complement(6098..>6502)
                     /locus_tag="PLX22_09225"
     CDS             complement(6098..>6502)
                     /locus_tag="PLX22_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20117.1"
                     /translation="EVSYILLSFTEIISTDVTVYSKFSKTAMLHAIKRGMNPDIFINT
                     LEQYTRNQFPQNIKFQLNEWIDHTPTITIQRGIILTSTTKGFIDELSHSQIKNVIVER
                     LGENHVMIHESQLDAVIKYAQKKEAVINIYLQ"
BASE COUNT         2337 a          931 c         1179 g         2055 t
ORIGIN      
        1 tgacagaata gagcattgta ccgctcacac ggtttttgcc tccggcagga tgatcacgat
       61 tacccttttg gtcgaacaag acatgttgaa agcggtctca ataattcagc gaatgaagtt
      121 acctacttgg ctgacaaaag catagggaac tcaatgttca atataggtat ttattaaata
      181 tttcattttg atcttgacta atgtttcgta aagtacctat catagtacac aaatttcact
      241 attatggatt tttcaggaaa actcaactat taccaataaa tataatggat gcagaaatta
      301 aaatccaaga tggcaaatca gacatgatta aagtcttcaa aataaaggat cgattaatga
      361 agattatcaa caagggatac ttctgccaat tcaggcagaa tctggataga ggaagtgctt
      421 cgtttatact cccaaaatat gatgaaaaac gaagtacttc gtttatgaat aagttgtacg
      481 caatggcaac aaaactttgg cctggcgtcc gtgcccggct tgttgtttat aaaattaacg
      541 tgccaaagga ggcccaaatg tcacaaaaaa aatctctttt taacaaattt tcaaccattt
      601 tcattatctt tttcaccctg cctattatta tatcttgtgg tgaaagcaaa gaaatgatcc
      661 gtcaaaggca agaaaatgaa ttgagagaaa aacagcgaca agaagctcta cagaaaagaa
      721 gagcagaaat tgatgcagta ataaatgatt taaaaacaca atatcaagat ccatttgacc
      781 tttcagatga aaaaaattgt gaattatttg ctagagccta taacaatcca gaagaaaatc
      841 ccaagattag aaattcgact gatacttcgg aaaatgaatt tcagtatcag aaagataaaa
      901 ttcaatatag tcaattacga aataaaaaat atgctataat gagttttaat atttttggta
      961 atgaattaca aaaatatgac tttcaaaaaa agtctttttc attacaattt aaatcatata
     1021 cgattatggc tcctgaaggt aatgtgattt ataccaaaaa aagaccacct gttgttaaat
     1081 tacttgtcaa aggtgttcct gatattgata ttaaaattga gcctgacctc gctgaaaatt
     1141 ttaaaagcgg aatcactaat acagcaactg gttttggtgc aatgtatggc ttagagtcac
     1201 aatataaaga ccgagccatt ttatatgtta ttttcaaaat caatggaaaa attttagatg
     1261 atgatattca gcatataagt gtagatattg cagattatgt ttgcttttgg tatattgatg
     1321 caggttcaag tatatcgcaa ggcggcagat acggaaattc acaatgttct gatataattg
     1381 aatgttcttc agatattaaa atatttcgta aatgaaatta tttggagtta aattatgacc
     1441 aaaattgaaa aattattgaa aattggacct tatcaacttg aggttacggg aattgatgac
     1501 ggcaggagct atatagtaaa tgttttttac aaaaaatatt atattccccc gacatattct
     1561 tataaccata ctgattttaa taatatcgtt aatagccaag aatcaggtct tttttatggt
     1621 aaattagatg atgtaatgtc tgattttata agaataattg aaactgacac tatgattgaa
     1681 aataaaatta aacaaattga taagtatata gaaagtaaat aaatatatct ccagccttcg
     1741 ccatccttgg ctacggctgg aataaacttt ttgttgttgt tggccgcatc acttaacaaa
     1801 gcttatgcgc ttcactcgct gatggtcatt cggactgcgc cacaattcgc atggcggctg
     1861 aacgttgcat gtgaaaaaca atatgctata ttataaacca taccatgccc aacttaacgg
     1921 gataactatg atactggatt agacggcttc gtactatcaa tgttgtagac gtcatataac
     1981 acgtagccat tcaatgtgct cattttgtca gttccggatt ctgcaacatt tttagctaca
     2041 ctgtattacg gtgaaaaatc gaatgtaccc taaaagttat gaaaaactgc cggtaaaact
     2101 attagtttgc cgtcaatgat gggtaagtgg gcaaatgaat ttatacgatc ataaagtatc
     2161 attattagcg agtataaata gagaacaaga aatacttgat aacaaaatta attttttaaa
     2221 cttacctata tcaaagataa accaaaaatc ataaattgta taatatgtat atataatttt
     2281 atatataatt atttacttgc aattatttat agtatattat catattgtca tatcagaatt
     2341 attttaatac tttagtattt tcacaatgat aacaagatgg ttttgcattt atgcttttta
     2401 ttataaatag tatcacaatg tattcataac tatattttat atgcaggagg tactatatga
     2461 aagatcctaa aaaggaccag gcgtgtatcg aaaggtataa ggagttgctt cctaaccttg
     2521 ctgattatgt agcgcattat gcaaagaaaa agccacaaga gctggcaata attgaacaca
     2581 atactggagc aaaggtaacc tggaaacaat ttaatacatc agtttcagcg tttgcagcaa
     2641 agcttttgtc cataggattg aacaaaggag atattattgc aacaagtttg ccattgctca
     2701 aggagcatgt ttatttgatg tatgcctgtt atcgttctgg cattattatt gcaccactgg
     2761 atttgcgctt aaagacaaaa gaaattcagt attgccttga tagaatgaaa cctaaagcgt
     2821 attttttcct gggcaaaact cctgtagctg atttccgtcc aatcattgca gaggttatga
     2881 aaacatctcc ctatataaag cactgggtac agttccaaac tgagccagaa ttcatcatgg
     2941 aaggtgcaat aggtattacc gactttgtta aagatattaa aacggtattt atcaaaagcc
     3001 tttttacagg cagtgtaaga agggctcgga aaaaagtgca gaaaagggat gccagcttga
     3061 tcatatttac taccggttct actggttcac ctaaacctgc attgttatgt catgaaggta
     3121 ttttaacaca gaatatagga ttggctattg gttttgaatt aagagaaagc gatagatttt
     3181 tggtaaacct tcctccatca catgtggggt gtacaaccga gcagctggca acaactatct
     3241 atggtggcgg cacatcggtt cttcttcata tatttgatcc aaaattaagt ctggaagcaa
     3301 ttcaaacgca taagataacc tgtatagggc agatacctgc attgtataat atggagtggt
     3361 tgcttccaaa ctataaagat tatgatctat caagtttgcg atttgcaatc tatggtgggc
     3421 aggcagtttc acgggagttt ctggttaaga tgaaaagcat gtccccgcaa attggaacag
     3481 ggcttggatt gaccgaaact tcaggatttt gcacttatac tgatgtgaat gcagatgttg
     3541 atgaaatagt aaagagtatt ggatatgata tgccgttgtg tccactcagt attcgagaac
     3601 ctatgaattc tgatggctca gcaggtaaag agaaatccaa aggagagata ggcgaaattt
     3661 gctttagtgg accacagatt tttcttggat atcttgggga tcctgaaaat acccgtaaga
     3721 ccatatctac cgatggtata tgttatacag gtgatctggg ctattatgat gaagaggggt
     3781 tacatttttc aggaaggtca aaactggtaa taaaacctaa aggatatcag gtgtatccag
     3841 atgatgttga acatcatata agtgagaagt tgaaaggtaa agtaaatatg gtagcgatag
     3901 ttggtgctga acataatgta tattccgaag caatcattgc atttgtagaa ccattgccgg
     3961 gagttgaggt aacaccagaa gatgttatga aagcatgcga agatatatca gcgtattcac
     4021 gaccttctca tgttgagatt attaagccag ggcagttccc attaaatagg gtagcaaaaa
     4081 cagattatgt tgtgttaaaa gatgaagcaa aaaaaattgt tgaaaaacta cgaagcgaag
     4141 gcaaatggga taaagcaaaa taataaaatt attatttaga ttagatgtta tcaaagaggt
     4201 tatctggcaa aattcattat tactattaaa tgaattgtaa cgatagcctc ttttttttat
     4261 tttaatacta caataaaaaa cagttgcaaa aattaattat tcttctaata tattggttga
     4321 gatataaaat catactataa atggtgttat gtatggaacg gactgatata tggtattgta
     4381 cagataatga taaaggaaaa gaaatatatg aaacattaaa attgcttggg ttgaatattg
     4441 tattggttga agataaaaat ttttcttcat gcaatgtact cccttcacag ataaatatat
     4501 ttattattga tcttgttact gtagaatttt cagaaatact gtctatgtta agaaaagatg
     4561 aacggttaca taaatttatt aaatatctta ttttatttaa aaaacaaata agaaaagcat
     4621 cccaactttc aattgatgta atgcatcttg aaatattatc gcgccccata aaaaaacgtg
     4681 aattttcatt attattagaa aagtctataa tcgttgaacg ttataaagaa atgctcaatc
     4741 tatattcgct tgaagcaacg gaacgcatag aagcatatac caacctcctt gatattaaca
     4801 gaaaagaatc tttttttgat gaaaaggaaa aaaatatttt ttcaaaaata ataaattttg
     4861 aaaagcatat ccttaaagaa cagaaagagc ttaatgtagc aattgccaat tataccaggc
     4921 tacgacaaaa ggagctattt gatttaaaga aaagaattga tgcggaagaa atgctttcag
     4981 agctaaggcg taaggaactg atggatgcta aaagtgttat tgaagctcaa agtgcagtta
     5041 tagaatattc aagtaaagaa ttacagagtg ctgaagaaat aattcatgct accgaagcag
     5101 tgcaagaact aagcagactt gaagccataa aacttcatga agagttagaa aaagaaaaaa
     5161 aaaagaatga ggtactgcaa aaaaaaattg atatgcttga gaaaaaaata aataattcca
     5221 aatcctaata atgaactgtc aattaatact aaaaattata attattattg tcttcgttcc
     5281 atcatgttca ttcgcatggt ttaaccgggg acttgatgct actttgatta caatgcaaaa
     5341 tctttttagt aaaagagatt taataacgct atacaatgaa tatgtccatc cggatatcaa
     5401 gagtgatgta ttaacattaa ttgatgcaat gaaaaatgaa aaaactgatg atagaaagat
     5461 tgctttacgt cttggctttg ctaatcttaa ccaatattat agcgcctcgc atcaggatat
     5521 tattaatcac ttttttttaa tgatggttag ttcacgatat actaaacctt cggatatcaa
     5581 aaatagtgct tatctcaggc ttgcaatttc tttatcgttg ctatttgctt ttcttgataa
     5641 tggtatggca ctcgttgaaa aagatattgg cttcagatgt gcaaaactaa cttataatgg
     5701 taatagcttc accatgaaga tatattttta ttatagtaat aaaaaatggt tgttgacaga
     5761 tggaaggcat tgtttataag aattgttata gcaataaaat aaaataatga ttgattagat
     5821 aataagtatg gaatggcaaa aaatattagt tgatgcagta caggacggaa aaataaaaga
     5881 attgcatctt aaaaaaattc ctgttttaaa gacctgtgat aattggcgtg aggtagaacc
     5941 catcgggtgg attgatcatc aaatgaagct tactcattat aaaggagcac ttgtgaggtt
     6001 aaggggtaat atttattttg tgacagaaaa aaccatcaat gctttgagcg aatatataaa
     6061 ttggaaaata tctcagcgta ttgatgtaat ataattatta ttgtaaatat atatttataa
     6121 cagcttcctt cttttgagca tatttaataa ctgcatcaag ttgcgattca tggatcatga
     6181 cgtggttttc acctaaccgt tcaacaatta catttttgat ctggctatgg gataattcat
     6241 ctataaatcc ctttgtagtg gaagtaagta taattcctct ctggattgta atggttggag
     6301 tatggtctat ccattcatta agctgaaatt ttatattttg agggaattga tttctggtgt
     6361 attgttctaa tgtattaata aaaatatcag ggttcatacc tcttttgatt gcatgtaaca
     6421 ttgctgtttt ggaaaatttt gaataaactg ttacatctgt tgagatgatt tcagtaaaag
     6481 atagcagtat ataggaaacc tc
//
LOCUS       DAOSOX010000133         6469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12048_length_6469_cov_7.507328, whole genome shotgun sequence.
ACCESSION   DAOSOX010000133 DAOSOX010000000
VERSION     DAOSOX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12048_length_6469_cov_7.507328"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..373
                     /locus_tag="PLX22_09230"
     CDS             56..373
                     /locus_tag="PLX22_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20118.1"
                     /translation="MKRYGTTPNRLSNATQKKVVHTGNNTKRIGIIIAMAIVMVIIAT
                     VIIRDCNQQKNVSAIKSVDIEPISKKHDNKRKQTTHKKAATPYEVPRDSSPPQTNLNI
                     ITD"
     gene            complement(402..>2106)
                     /locus_tag="PLX22_09235"
     CDS             complement(402..>2106)
                     /locus_tag="PLX22_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20119.1"
                     /translation="FSNNAIYIVMQGKTLSGGLLSDDLFFLYSTNNGKSFSNPKKITT
                     SPANDSSPSIILHNDVIYCVYQNNENRQWGIKLLRGFDKGKNWETQPFNVTTVNVNCF
                     QPRVVAASNDDLLIAWYDARERYEAVFAKKFSLIDNTFTSEVRISDPNVNSNDQFPVL
                     SGQRILLFWNSNERLIAKFSDISVLPPRIYSVTHPEGRWTRSTTAILEWVPPVDESGI
                     SGYAVIYNELPAFDPAVQNIEGPIRKYIVTDLKDGVTYFHIRAIDRAGNYSRTIHYPL
                     MVSSTPLPMPMVSSPTHPEGTQINNPSPVLQWTIDEDERVKGFYYSIAKDAIKEPSDF
                     TTANTVEFNNLPQGRYFFSLRPIDKTNMKGRIATYEIVIGNVEKADAEYYEKLAKGIV
                     QPEEKPDEGMVVQRKYPSVVINIPFKEIYATHFYRFQLIPRNITPQTVDGFSVIVANN
                     KEVPPFFISHNEGDVTLTNVKPGKYVIAARMRYSDYQNGKRVYRWTKPCYRELMVKSA
                     HTSPLMHYADNLYVMIKDRQLITAIYSVGLLAIVLIVGMSARISFSFQKLLYRVSLLF
                     R"
     assembly_gap    2107..2206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2207..2645)
                     /locus_tag="PLX22_09240"
     CDS             complement(<2207..2645)
                     /locus_tag="PLX22_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20120.1"
                     /translation="MKRFFTYLFELTFRKPKLRYFVWGVIIFLLIYGSFYLFYQEVVT
                     FPVGWEKSVIITPYTLKIKSPYAVSKGNVIVAAFEGNEKNESGVYITVSLNAGHDFLP
                     PYKLDTIDPAVAIRPHVAISNNGHIACTWHNLINNRSAIMLTIS"
     gene            complement(2664..4385)
                     /locus_tag="PLX22_09245"
     CDS             complement(2664..4385)
                     /locus_tag="PLX22_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT20121.1"
                     /translation="MYEKKPWLKFYGDVPHTLEYPKVTMYEALMNTVSRKPNAIAWDF
                     MGHTATYKEFSKLIDQCARGLYALGLREGDTITISMPTVPQGIICFYAVNKIGAVASM
                     IHPLSPPKEIEFYLTISNSKFALTIDAFYNAFNEIMDKTPCKTLILAKISDYLPALKA
                     LGFKLTKGRKIPKVPQDPRVVWWNDVMKQSSGDVPKSNMKTDDMAVILYSGGTTGMPK
                     GIMLSNMNFISEGKQVAAWGRMSEGDAVLAILPIFHGFGLGVCVNAFFMGGGKSILVP
                     QFDAVVIAKLIKKTKPNFLIGVPTLFEALSKSKVIQSADLSCIKAAFSGADTLPRVVK
                     DEFERVVAQQGGNAKLREGYGLTESVTAIMAMPMNEYREGSIGIPFPDMMAKIVKTGT
                     IQEAPWGEEGEICIHGPAVMIGYLNQPEETAKTLRKHDDGLVWLHTGDIGTMDEDGFF
                     YFKLRLKRMIKSSGFNVYPTQVEDVLRQHPAVDQACVIGVPDKAQVERVKAFVVLKPE
                     YKDKKSDDLVQELIKHCREHLIKWSCPREIEFRDELPQTKVGKIAFNVLEDEEKEKLK
                     AKGEYTG"
     gene            complement(4574..5809)
                     /locus_tag="PLX22_09250"
     CDS             complement(4574..5809)
                     /locus_tag="PLX22_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOT20122.1"
                     /translation="MSGEMQDKKDNRIKSVKRMKEIMTNYYIDAKTAAQNNKKVGWIT
                     SGGPVEPLLVMDVIPVYPENHGAMIGAAKMGVQLCEKSEEMGYCRDLCSYARSDIAAA
                     PINGGPIGGLPKPDFLICCNNICGTVLKWYEVQARYFKVPLFIFDTPFCHKGITQEAK
                     DYVGAQIVEYITFLEEHCGKKFDYDKAKEVGKLSIQGQRLWQAVLDTTMHKPAPMSAF
                     DAFFHLALIVTLRGTQIAVDYYTELLQEMKERNAHGISAIPNEKYRLLWDNLPVWYKT
                     KWLSETFASHDACLVADTYTTAWCRQLDYLDENDFLKSMIEGYTAIYLNIGVDQMADA
                     VIGMVDKYDVDGLVMHSNRSCKPYSLGQYDIQRIVQQKRNIPSLIIEADMLDERNFSE
                     SQILTRIEAFMEIIKNKRR"
     gene            complement(5824..>6469)
                     /locus_tag="PLX22_09255"
     CDS             complement(5824..>6469)
                     /locus_tag="PLX22_09255"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT20123.1"
                     /translation="EKLEKAISTYNEIRSLLKTIWQLKSDNKSTLNGSQMNALIKAAM
                     VWDRDELAGMLKNLIPSIELSPKNEKAKRIMLIGGICNHPDIYTYIEDSGGVVVWDDL
                     CTGSRYFEGQIATDSNPLQAIANRYYERNICPAKHRDLNTRVETITSMIREHNVQGAI
                     FLYLKFCDPHSFDYPYIKEALDTMGIPSTLLELELQLPSEGQMKTRLEAFIDMI"
BASE COUNT         1924 a         1392 c         1030 g         2023 t
ORIGIN      
        1 atggcaatcc caacagtatc acggtaaatc aataacataa ctatcccata atatcatgaa
       61 gcgctacggc acaactccca acagattatc aaatgctaca caaaaaaagg tagtacacac
      121 tggtaataat actaaacgta ttggtataat aatcgctatg gcgatagtta tggtaattat
      181 tgcaacagtt ataataaggg attgtaatca acaaaaaaat gtctctgcca ttaaatcagt
      241 agatattgaa ccaatttcaa aaaaacatga taataaaaga aagcaaacta ctcacaaaaa
      301 agcagcaaca ccgtatgaag tacctcgcga cagcagcccg cctcagacca atttaaatat
      361 cattaccgat taaatttcat catggattac ttgtaaagta actatcgaaa caacaaagaa
      421 acacggtaca gcaatttttg aaatgagaat gatattcggg cactcatccc aacaataaga
      481 acaattgcca gtaatccgac actgtaaatt gctgtaatta attgcctgtc tttgatcata
      541 acataaagat tgtcagcata atgcataagc ggtgatgtat gcgcagattt aaccatcaat
      601 tccctgtagc atggtttagt ccaccggtac actcttttcc cattctgata atcactatac
      661 cgcatgcgtg ctgctataac atatttgccg ggctttacat tggtcaatgt aacatcgcct
      721 tcattatgtg aaataaaaaa tggaggaact tctttgttgt ttgccacaat cactgaaaaa
      781 ccatctactg tttgtggcgt tatattccgg ggtattaact gaaacctgta aaaatgtgtt
      841 gcatatattt ctttaaaagg tatattaatg acaactgatg gatattttct ttgaacaacc
      901 atgccctcat caggtttttc ttcaggctgt actatccctt ttgcaagctt ttcataatat
      961 tcagcatctg ctttttcaac attaccaata actatctcat aggttgcgat ccttcctttc
     1021 atattggttt tatcaatagg acgtaaactg aaaaaatatc tgccctgtgg taaattgtta
     1081 aactcaacgg tattggctgt agtaaaatct gaaggctctt taattgcatc ttttgcaatt
     1141 gaatagtaaa aaccttttac tctttcatct tcatcaattg tccattgtaa taccggagaa
     1201 ggattgttta tctgagtacc ctcagggtgc gtgggagagc tcaccattgg cattggcaat
     1261 ggtgtactgc ttaccataag cggataatga attgttcggc tataattgcc tgccctatca
     1321 atagccctga tgtgaaaata ggtcacccca tcttttaaat cagtaactat gtatttgcgt
     1381 ataggacctt caatattttg tacagctgga tcaaatgcag gaagttcatt ataaataaca
     1441 gcatatcctg aaatacctga ttcatctacc ggtggcaccc attccaatat tgccgttgtg
     1501 ctgcgtgtcc acctgccctc cggatgagta accgaatata tcctgggcgg caatactgat
     1561 atatccgaaa attttgcaat cagcctttca ttgctgttcc agaataataa gatgcgttgt
     1621 ccactcaaga ccggaaattg atcattagag ttaacattgg gatcggatat tcttacttca
     1681 ctggtaaatg tgttgtcaat aagtgaaaat tttttggcaa aaacagcttc ataacgctct
     1741 cgtgcatcat accatgctat gagtaaatca tcgttagatg cagcaacaac tcgtggctgg
     1801 aaacaattaa cgtttacagt tgtaacatta aaaggttgag tttcccaatt ttttccttta
     1861 tcaaatccac ggagcaactt tatgccccac tgacgatttt cattgttttg ataaacacaa
     1921 taaataacat cattatgtaa aatgattgaa ggtgatgaat cattagcagg acttgttgtt
     1981 atttttttag ggttagaaaa acttttccca ttattagtag aatataaaaa gaataaatca
     2041 tctgacaaca aaccaccact gagtgtttta ccctgcatta caatatatat tgcattatta
     2101 ctgaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttga tatggtaagc
     2221 attatggcac ttctgttatt aataagatta tgccatgtac atgctatatg cccattattt
     2281 gatattgcca catgggggcg tattgctact gccggatcaa tggtatctaa tttgtaggga
     2341 ggtaaaaaat catgtcctgc atttaatgat acagtaatat acacaccgct ttcgttcttt
     2401 tcgttacctt caaaagcagc aacaattaca tttcccttgc taaccgcata tggtgattta
     2461 attttcagcg tatagggtgt tatgataaca cttttttccc atcccacagg aaatgtaacc
     2521 acctcctgat agaacaaata aaaactgcca tagataagca aaaatataat aacaccccat
     2581 acgaaatatc gcagctttgg tttgcgaaat gttaactcaa ataaatatgt aaaaaatcgc
     2641 ttcatactcc cattatcgga tatttagccg gtatattcac cttttgcttt tagcttttcc
     2701 ttctcttcat cttcaagtac attaaatgct attttcccaa cctttgtctg tggcaattca
     2761 tctctaaact ctatctcacg tgggcaactc cattttatca agtgctccct gcagtgtttt
     2821 atcaattcct gtactaaatc atcactcttc ttatccttat attctggttt taaaacaaca
     2881 aatgccttta ctctttccac ctgtgcttta tcaggtactc caataacaca tgcctgatca
     2941 actgcagggt gttgccgcaa aacatcctca acctgtgttg gatatacatt aaaaccagac
     3001 gattttatca tccttttaag tcgtaactta aagtagaaga agccatcttc atccattgtg
     3061 ccaatatcac ctgtatgcaa ccataccaaa ccatcatcgt gtttgcgtaa tgttttagca
     3121 gtttcctcag gttgattgag atatccaatc ataacagcag gaccatgaat acaaatttca
     3181 ccttcttctc cccatggagc ctcctgaata gttccagttt ttacaatctt tgccatcata
     3241 tcaggaaatg ggattcctat gctgccctcc ctgtattcat tcatcggcat tgccataata
     3301 gcagttactg actcggttaa tccataacct tcgcgcaatt tagcatttcc tccctgttgt
     3361 gctacaactc tttcaaactc atctttgaca acccttggca atgtatctgc accactgaat
     3421 gcagctttta tgcatgacaa atctgctgat tgaataacct tgcttttact taatgcttca
     3481 aataatgttg gcacaccaat taaaaagttt ggctttgttt ttttgatcaa ttttgcaata
     3541 acaactgcat caaattgagg caccagtatg cttttaccac cacccataaa gaaagcatta
     3601 acacaaacac ctaaaccaaa tccatggaat attggtaaaa ttgccagcac tgcatcaccc
     3661 tccgacatcc tgccccatgc tgctacctgc tttccttcgc tgataaaatt catattggaa
     3721 agcataatgc ctttgggcat ccctgttgta ccgccactat agagtatcac agccatatca
     3781 tcagttttca tattggattt tggaacatca cctgaagatt gtttcattac atcattccac
     3841 catacaacac gaggatcttg tggtaccttt ggtatttttc taccctttgt tagcttaaat
     3901 cccaatgctt taagtgccgg taaataatca gaaatctttg caagtatcaa tgttttacat
     3961 ggggttttat ccataatttc attaaatgca ttatagaatg catcaatcgt taatgcaaat
     4021 ttgctattgc tgatagtaag ataaaattct atttcttttg gtggcgatag tggatgaatc
     4081 atactggcaa cagcaccaat cttattaaca gcataaaaac agataatccc ctgaggcact
     4141 gttggcatcg agatagttat tgtatcacct tctcgtaatc ccaatgcata taagcctctg
     4201 gcacactggt caataagttt tgaaaattcc ttataggtag cagtatgccc cataaaatcc
     4261 catgcaatag cattgggttt acgacttacc gtattcatga gtgcttcata catcgtaact
     4321 tttggatatt ctaaagtatg aggtacatca ccataaaatt taagccatgg ctttttttcg
     4381 tacatatata atctcctgca aaaatataat tattgctttt cgattcgatc ctgaatcaag
     4441 gttcagaatg acatgctacg tttccgggac gacattctca atttcaggat gactatcgcc
     4501 aattattttc attccttgtt ggtagccata agcaagccat cgttaactcc cctaacaaga
     4561 aaaagatgag attctacctt cttttattct taataatttc cataaatgct tcaattctgg
     4621 taagaatctg gctctcactg aaattacgtt catcaagcat atctgcctca ataatgagtg
     4681 aaggaatatt acgtttttgc tgcacaatac gctgtatatc atactgtccc agtgaatacg
     4741 gtttacagct tcggttagaa tgcataacca gtccatcgac atcatatttg tcaaccatac
     4801 caataactgc atctgccatc tgatccactc caatattaag atagatggct gtatatcctt
     4861 ctatcatgct cttcaaaaaa tcattttcat ccagatagtc taattgtctg caccacgctg
     4921 ttgtataggt atccgcaaca aggcaggcat catgcgatgc aaaggtttcc gagagccatt
     4981 tcgttttata ccatacaggc agattatccc acaataggcg gtatttttca ttgggtattg
     5041 cactgatacc atgagcattg cgctccttca tctcctgtaa aagttcagta tagtaatcca
     5101 ctgctatttg tgtgccgcgt aatgtaacaa taagagccag atggaagaag gcgtcaaatg
     5161 cactcatggg tgcaggtttg tgcatggttg tatcaagtac cgcctgccat aaccgttgcc
     5221 cttgaattga aagttttcca acttctttag ctttatcata atcaaatttc ttaccacaat
     5281 gttcttctaa aaaggtaatg tactcaacta tctgtgcccc tacataatct ttggcttcct
     5341 gagttattcc cttatgacaa aacggcgtat caaatataaa cagcggaacc ttgaaataac
     5401 gagcctgcac ctcgtaccat ttgagcaccg taccgcaaat attattgcaa caaatcaaaa
     5461 aatcaggctt tggcaaacca ccaattggac caccattaat tggtgcagca gcgatatcac
     5521 tgcgtgcata cgagcacagg tcacggcaat agcccatttc ctcagatttt tcacacaact
     5581 gcacacccat ttttgcggcg ccaatcatcg caccatgatt ttctggatat accggtataa
     5641 catccattac cagaagaggt tccacagggc ctccgcttgt aatccatcca accttcttat
     5701 tattttgagc agcagtcttt gcatcaatat agtaattggt cattatctct ttcatccgtt
     5761 taacggattt tattctgttg tcctttttat cctgcatttc acctgacata gtatcacctc
     5821 actttaaatc atatcaataa atgcttcaag tcgtgtcttc atctgccctt ctgacggcaa
     5881 ttgaagctct aattccagca atgttgacgg aatacccatt gtatccagtg cctctttaat
     5941 atagggataa tcaaatgagt gaggatcaca aaactttaaa tacaaaaata ttgcgccctg
     6001 aacattatgc tcccgtatca ttgatgtaat tgtctcaaca cgggtattca gatctctatg
     6061 cttggcaggg catatattcc gctcataata ccggttggca atagcctgca gtggattgct
     6121 atcggttgct atctgcccct caaaataacg agagccagta cacagatcat cccacaccac
     6181 cacgccaccg gaatcttcaa tataggtata aatatcagga tgattgcata tacccccaat
     6241 aagcattatg cgttttgcct tttcattttt gggcgatagt tctattgaag ggattagatt
     6301 ctttaacata cctgcaagtt catcacggtc ccacaccatt gcagctttaa tcagtgcatt
     6361 catctgactg ccattcaacg tacttttatt atcactctta agctgccaga tagtttttaa
     6421 aagcgaacgg atttcattat aggtactgat agctttttca agcttttca
//
LOCUS       DAOSOX010000134         6467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12052_length_6467_cov_5.856363, whole genome shotgun sequence.
ACCESSION   DAOSOX010000134 DAOSOX010000000
VERSION     DAOSOX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12052_length_6467_cov_5.856363"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLX22_09260"
     CDS             <1..484
                     /locus_tag="PLX22_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOT20124.1"
                     /translation="IVIAGDSAGANLATVTCLQCKDNLLPLPQLQVLFYPVVAADLCS
                     ESLRQYGNGFFLTKASMLWFTQLYVASTDDLLNPYFFPILHDNLTGSPEAVIITAEHD
                     PLCNQGEMYAVALQESHVDALCIQAKGMIHGFLNFSYLIPSAATLADMVWAIVGKKLR
                     "
     gene            complement(512..3637)
                     /locus_tag="PLX22_09265"
     CDS             complement(512..3637)
                     /locus_tag="PLX22_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOT20125.1"
                     /translation="MINTWIQKALHGQLWVILITVAVIVGGAFAIGRLPIDAVPDITN
                     VTVMVNTKTGALAPEEVEKTVTFPIESELAGLPDVQDIRSLSKYGLSQVIVVFSDDTD
                     IYFARQLVNERIQSIRDKLPEGLSPELGPVSTGLGEIFMYAVVAKKGSVLSQLSQKEQ
                     LTYLRTVQDFIIKPAIKSVPGVAEVESNGGYKKEIHIDVDPQKMDIYGITCHQMVKAL
                     EKVGENYGGGYIQVEGKQIIVRTYGKIDALQQIKNLPVRINVYGAPIRVWQVAEVVEG
                     NQQRLGAATFNGEEAVLGTVLMRINANSREVSQAIEKYISEISLPPDVQIKTLYTRSF
                     LVNATIKTVARNLTEGGLLVIAILFLILGSAKAAFLVALAIPVSMLFATSGMVISGIA
                     ASLMSLGAIDFGLIVDGSVVMVENIMRHLNLAKGKLQNKKEKMRIIGDAAKEVSQPVF
                     LGILIIMAVYVPVLSLTGIEGKMFRPMAETVLYALGGSILIALFLMPVMSYFFINPSH
                     SEKEPLFFRVLSKAYKPFLLYSLKHPVKVIIPTLLVALIAIIMFFNLGSDFIPRLDEG
                     DMVIGLVRDQTIGIDKSLEIQKKSDRAIAQFKEVTTVFSRMGTPESATDPMGVNFADT
                     FVILEKDKSKWQKYNGKTVSKVELFEIIAKKIENEAPDQEISLTQPIEMRFNEILEGN
                     RADITLRVIGQNLDILYELSNKSIPILEEIQGASTVELDPLTALKKSDIMDVVLDYDA
                     ISRYGVNINDVNNAFSMAMTGSYVGYLYDEDKRFPIVVRLGDKFRKNIADIAKIPVGL
                     PDGGTIPINTIAKIEQKKQITTIARTRAKRYAAVSINIKNRDTQSFVDEAKEKIKKLA
                     IPKGYQLNWGGQFKNLASARTRLLVIIPIVLAVIFVILIRTFHSITQTLLVFNSIPFA
                     LTGGVIFIYLRNIPLSVSAGIGFIALIGIAILNGMVLVNFFNQLRQEGMKLEKAVHEA
                     TMIRLRPVLMTALVASLGFIPMAYNTGIGAEVQRPLATVVIGGLVSSTILTLLLLPTL
                     YIWVEKLFSKD"
     gene            complement(3634..4878)
                     /locus_tag="PLX22_09270"
     CDS             complement(3634..4878)
                     /locus_tag="PLX22_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT20126.1"
                     /translation="MRRIANTIMLFLLMFYCYAFAQQNYSIKELVDSAFQTFELMKAQ
                     QLKIDEMEALRKYNTQWKNPEISAYYGSKSIESHKGDVAGVSIVQPLYFPGKISFVDE
                     IYRYKKNYEALSYEEMKYFIASTVINLSYDYAIKTLHSDHINSRLNRLKIINAYMSGR
                     PAISPQKRVEIAIVKNTIRMLESEIYKLKADKATTFARLALYTRIASPQLSLHINWIT
                     NPPLYDINAIEQKAKKESLLVKKQKQVYMASVAELSLSKKNIFPDFSILFNYDYEKVI
                     DNEKTISGGISIPIPLYNQNQNEIKAMQSRVKQEEFISHYVEKMIETDIRTIMVQYDY
                     YRNLLGLYSPDLEDELNRSMVYADNQFKRGTITLQSYLELDVQVHETLENIYTTQKEL
                     VRNISAIACLINDYTILLGIAQ"
     gene            complement(4915..>6467)
                     /locus_tag="PLX22_09275"
     CDS             complement(4915..>6467)
                     /locus_tag="PLX22_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT20127.1"
                     /translation="KNKKPFIGSFVFVPAIKKSLAVCAYPANNAWYCVAFDLHTLFSK
                     RYSHYRIGNRGYILLMDKNGIFLSHPHEQYIGKENLQQYDWGRKAFATQSVVKYPWEG
                     ERKIITVAISDVPAFSVAQTVYEQEIAMTIKNIIVFIIIISIGIIILGIIAIIVILTS
                     SFKPFTTLRDSIYRIAEGDISATPDVIYGNEIGNIATSIKILTSKISGVIDQIKNSSN
                     ELSTASEQMSAGISALSDVASNQSASTQEITATIEEISANLDSIVMNTKEESKSLSLF
                     STHLQELSQSIFSFLLIMEESLNRTHTISSEAQQGQVVLQQMNTSMKKIVDSSHGIAN
                     VAQIIRGIADQVNLLALNAAIEAARAGEAGKGFAVVADEITKLADAIAQSLKDIDNLV
                     RANEKEIQQGIGITENTVATITNVIQGIESVNLDVNTMGQKIREQSEIFNETKKVWDV
                     TESLSRQILDSVEDQKSAVEEIVRSINTINSLTQNVAGTAEEMHSSAENLASMAEQLK
                     IMLDYFKL"
BASE COUNT         1916 a         1318 c         1061 g         2172 t
ORIGIN      
        1 aatagttatt gctggagata gcgcaggtgc aaatttagca acggttacct gccttcaatg
       61 taaggataac cttcttccac tgccacaatt acaggtgctt ttttatccag tagtggctgc
      121 agatttatgc tcagaatcat tgcgacaata tggcaatgga ttctttttaa caaaagcatc
      181 catgctatgg tttactcagc tctatgttgc atcaacagat gatttgctca atccttactt
      241 tttccctatc ttacatgata atttaaccgg aagtcctgag gctgtcatta taactgctga
      301 acatgacccg ctttgtaatc agggtgagat gtatgctgtt gctctacagg aatcacatgt
      361 tgatgctctg tgtattcaag caaagggcat gattcacggt tttttaaatt tttcctatct
      421 tataccttct gctgccacat tagcggatat ggtatgggca atagtcggaa aaaaattacg
      481 gtaacgtttc attcaatatg attttaatta tttagtcttt gctaaatagt ttttctaccc
      541 aaatatataa tgttggtagc aataataagg taagtattgt tgaagatacc aaacccccaa
      601 tgactactgt tgcaagtggg cgctgtactt cagcaccaat accagtatta tatgccatag
      661 gaataaatcc taagcttgca accagagcag tcatgagtac cggtctcagc cgtatcatgg
      721 tagcttcatg tactgctttt tccaatttca tgccttcctg gcgtaattga ttgaagaaat
      781 taaccagcac catgccattg agtatagcta taccaattaa ggctataaat ccaatacctg
      841 cagaaacact taacggtatg tttcttaaat atataaaaat aactccgccg gtaagtgcaa
      901 atggtatgct gttaaatacc aataatgttt gtgttatact atgaaaagtt ctaataagta
      961 taacgaagat tactgccaat acaattggta tgataacaag taagcgtgtg cgtgctgatg
     1021 caagattttt aaattgtccg ccccagttga gttgatatcc ttttggaata gcaagctttt
     1081 taattttttc cttagcctcg tcaacaaagc tctgcgtatc acggtttttt atgttaatgg
     1141 aaactgccgc atagcgcttt gccctggtac gagcaatcgt tgttatttgt tttttttgct
     1201 ctatttttgc aatagtattg atagggattg ttccaccatc aggcaatcca acggggattt
     1261 ttgctatatc ggcaatattc tttctgaatt tatcacccaa ccgtacaact atagggaaac
     1321 gcttatcctc atcatacaga taacctacat aacttccagt catagccata gaaaatgcat
     1381 tattcacatc attaatattg actccatacc gtgaaattgc atcataatcc agcactacat
     1441 ccataatatc actttttttc agcgctgtta acggatccag ttctacggta gaagctccct
     1501 gtatctcttc taatattggt atggatttgt ttgaaagctc atataatata tccagattct
     1561 ggccaataac gcgcagggta atatcagccc tgtttccttc aaggatttca ttaaaacgca
     1621 tctcaatagg ttgcgtaagt gatatttcct gatctggagc ctcattctct atttttttag
     1681 ctattatctc aaaaagctca accttggata cggtttttcc attatacttt tgccattttg
     1741 atttatcttt ttccaatata acaaaggtgt ctgcaaaatt aactcccatt ggatcggttg
     1801 ctgattcagg agtccccatt cgagaaaata ctgttgtaac ttctttaaat tgtgctattg
     1861 ctctatcact ttttttctgt atttccaggg atttatctat accaatagtt tgatcacgca
     1921 ccaaacctat aaccatgtcc ccttcatcaa gtcggggaat aaaatcagaa ccaagattaa
     1981 aaaacattat tattgcgatt aatgccacta aaagagttgg aatgataact ttaacaggat
     2041 gtttaagaga atatagtaaa aaaggtttat aggcttttga taacactctg aaaaacaacg
     2101 gctctttttc agaatgagaa ggattaataa aaaagtaaga cattaccggc ataagaaata
     2161 aagcaatgag aatactccca cctaaagcat aaagcactgt ttcagccata ggtctgaaca
     2221 tctttccttc aatacctgtt aaactcagta caggtacata caccgccatt attataagta
     2281 ttcctaaaaa aaccggttgg cttacttctt tagcagcatc tccaattatc cgcatctttt
     2341 ctttcttatt ttgcaatttc ccttttgcta aattgaggtg ccgcattata ttttctacca
     2401 taacaactga gccgtcaaca ataagaccaa agtcaattgc accaaggctc ataaggcttg
     2461 cagcaatacc tgaaatcacc atgccacttg ttgcaaacaa catggacact ggtatggcca
     2521 atgctactaa aaatgctgcc tttgcacttc ccaagataag gaagagtatt gctataacca
     2581 gcaatccacc ttcagttaaa tttcttgcaa ctgtttttat tgttgcattt accaaaaagc
     2641 ttcttgtata caatgtcttg atctgaacat caggaggcag ggaaatttcc gatatatact
     2701 tttcaattgc ctgcgaaact tctctgctat ttgcattaat gcgcataaga actgtaccca
     2761 gcactgcttc ttcaccatta aacgtagctg cacctaaacg ttgctgattc ccttcaacta
     2821 cctcagcaac ctgccataca cggattggag caccataaac gtttatccta accggtaagt
     2881 ttttgatctg ctgtaatgca tcgatcttcc cataggttct tactataatc tgtttccctt
     2941 caacctgaat gtagccccct ccataatttt caccaacctt ctctaatgcc tttaccattt
     3001 gatgacaggt tataccataa atatccattt tttgtggatc aacatctata tgaatttcct
     3061 ttttgtaacc accatttgat tcaacttcag ccacgcccgg cacacttttt atagcaggtt
     3121 taatgataaa gtcctgtact gttctaagat aggttagctg ttctttttgt gataactgtg
     3181 agagaacact ccccttttta gcaacaacag catacatgaa aatttcacca agccctgttg
     3241 ataccggtcc aagctcaggc gataaaccct ctggcaattt atcacgaata gattgtatcc
     3301 tttcattgac caactgtcgc gcaaaataaa tatctgtatc atcagaaaac acaacgataa
     3361 cctgtgaaag accatatttt gataaagaac gtatatcctg cacatcaggc agtcctgcta
     3421 attctgattc aatagggaat gtaacagtct tttccacctc ttccggtgca agtgcacctg
     3481 ttttagtatt taccattact gttacattgg taatatccgg aacagcatca attggcaacc
     3541 ggccaatagc aaaagcacct ccaacaataa cagctacagt aataagaata acccataact
     3601 gcccatgcaa tgctttttgt atccatgtat taatcattgg gctataccaa gcaaaatagt
     3661 ataatcatta ataaggcatg caattgctga tatgttacgt acaagttctt tctgggtggt
     3721 atatatgttc tcaagggttt cgtgaacctg tacatcaagc tctaaatatg actgcaacgt
     3781 aattgtccct cgcttaaact gattatcagc atataccatt gatctgttta attcatcttc
     3841 aaggtcaggg ctatagagtc ccaacaggtt acggtaatag tcatattgta ccatgatagt
     3901 acggatatca gtttctatca ttttttccac atagtgtgaa atgaattctt cctgtttaac
     3961 tctggactgc atggctttta tttcattctg attttgatta tacaaaggta ttggaatact
     4021 tatccctcca gatatagttt tttcattatc aattaccttt tcataatcat aattaaatag
     4081 aatgctgaaa tcagggaata tatttttctt tgacaatgac agctcggcta ccgacgccat
     4141 gtatacctgc ttttgctttt taactaatag gctttccttt tttgccttct gttcaattgc
     4201 attgatatca tacaatggag ggtttgtaat ccagttaata tgcaacgata gctgtggcga
     4261 agcaatcctt gtatataacg caagacgggc aaatgtagta gccttatcgg cttttagttt
     4321 atatatttct gattcaagca tccgtatagt attttttacg atagctatct ccacgcgttt
     4381 ttgtggtgat atggcaggcc tgcctgacat atatgcatta attatcttta accgattgag
     4441 gcgtgaattg atatggtctg aatgtaatgt tttgattgca tagtcataac tcaaattaat
     4501 gaccgttgaa gcaatgaaat atttcatctc ttcatacgat agtgcttcat aatttttttt
     4561 ataccggtat atttcatcta caaacgaaat cttcccggga aaatacagcg gttgtacaat
     4621 tgaaacacca gcaacatccc ctttatgcga ttcaatactt ttgctaccat aatatgctga
     4681 tatttctgga tttttccact gagtattata ttttctgaga gcctccattt catcaatttt
     4741 tagctgctga gctttcatta attcaaatgt ttggaaagca ctatcaacta attctttgat
     4801 ggaatagttc tgttgtgcaa acgcataaca gtaaaacatt aataaaaata gcattatagt
     4861 attagctatc cttctcatgt atattcctat ataagaattt ttaaaattgt actatcataa
     4921 tttaaaataa tcaagcatta ttttaagctg ttctgccatt gatgctaaat tttcagcgct
     4981 tgaatgcatc tcttcagccg tacctgccac attctgtgtt aatgaattaa tagtattaat
     5041 tgaacgtaca atctcctcaa cagcgctttt ttgatcctct accgaatcca atatttgacg
     5101 tgatagtgat tcggttacat cccatacctt ctttgtttca ttgaatattt cactttgttc
     5161 ccgtattttt tgtcccatgg tgttgacatc cagatttacg gactctatcc cttgaatgac
     5221 atttgtaata gtagcaacgg tgttctctgt aataccaatc ccctgctgaa tctctttctc
     5281 gtttgctcgt actaaattat ctatatcttt taaactctga gctatcgcgt cagctaactt
     5341 cgtaatctca tctgcaacca ctgcaaatcc ttttccagct tcacctgctc gagcagcctc
     5401 aattgcagca ttgagtgcca ataaatttac ctgatcagct atgccacgaa taatttgggc
     5461 aacattagca attccatgag aactatcgac aatttttttc atacttgtat tcatctgttg
     5521 cagtactacc tgcccctgtt gcgcttcact gcttatggta tgcgtgcgat ttaaagattc
     5581 ctccatgata agcaaaaaag aaaatattga ttgcgatagt tcctgtaaat gtgtagaaaa
     5641 aagagaaaga ctttttgact cttctttggt attcatgact atggaatcta aatttgcaga
     5701 gatctcttca atagtagctg ttatctcctg tgtagaagca gactgatttg atgcaacatc
     5761 tgaaagtgcg ctgataccag cagacatctg ttcagaagct gttgacaatt cattggaaga
     5821 attttttatc tggtctatga caccactgat tttgctggtc aatattttta tagatgtagc
     5881 aatgttgcca atctcattgc catagatgac gtccggtgtt gctgatatat ccccttcagc
     5941 tatccggtaa attgaatcac gtaatgtagt aaatggttta aatgaactgg ttaatattac
     6001 aatgatagca ataatcccta aaattataat tcctatggaa ataataataa taaacactat
     6061 tatatttttt atagtcatgg ctatttcctg ctcatatacc gtctgagcta cggaaaaagc
     6121 aggaacatcg ctgatggcta cagtaatgat ttttcgctca ccttcccacg gatatttaac
     6181 tactgattga gtggcaaatg cctttctacc ccaatcatac tgctggagat tttctttgcc
     6241 aatatattgt tcatggggat gtgacaaaaa gataccgttt ttatccatca atagtatata
     6301 tccacgattt ccaatacgat aatggctata gcgtttagaa aatagtgtat gcagatcaaa
     6361 tgctacacaa taccatgcat tgttagcggg gtaagcacat actgctaacg atttttttat
     6421 tgccgggaca aaaacaaaac tgcctataaa tggtttctta tttttga
//
LOCUS       DAOSOX010000135         6464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12060_length_6464_cov_7.573100, whole genome shotgun sequence.
ACCESSION   DAOSOX010000135 DAOSOX010000000
VERSION     DAOSOX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12060_length_6464_cov_7.573100"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="PLX22_09280"
     CDS             complement(<1..1855)
                     /locus_tag="PLX22_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT20128.1"
                     /translation="MLLYTTNIRSSKGTRIIIFLFLKFIAFNFNNRKSLNRYLTSKDG
                     WLNFSIGLKTEDNSLQYGISFFEGKVAVTKTIAHDAKAVMIFKNDEAARKLLLGTPTD
                     VIYMLLKSDVRIEGSISYANIFSFFISLLFHDVQKRAMKTERKKDDKTVKRQQPEHNK
                     QLSDEIVKRRKERLKAEGADPHVVYLDDAYLSKYSMDDFPRLQRFLHDHLTKKPAVCA
                     ERAYLLTKWYRENGFEKDSSGNNWDPIVRNALAYKYMMENRKPLINDESLLAGTTTSK
                     PIGCPIYPDGSGIMIWNELMTISYRTYNPFDIDEQTREVLHFDVFPFWLHRNFREWVR
                     DKYNNPLSQQLDERYALYFNWKQATISHTIPNFPKVLAMGTLGMIQEMHEELNSGCGE
                     EKQNCLTAMILCLEGVNAYAQNLSKQAMGDSNREQNPQRKQELLTIADICSRIPMHGA
                     QTLHEAVMAVWIIWIALHMESMNAGLSLGRLDQWLQPYFVNDMKNIPPEKRDEYMKFA
                     LELIGDFYMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPEGTDAVCDMTYIFLKV
                     TEMLSVRDPNVNARYMPGVNSDTYLKRLCEVNLITAATPSMHNDAAIIKALT"
     gene            complement(1945..2580)
                     /locus_tag="PLX22_09285"
     CDS             complement(1945..2580)
                     /locus_tag="PLX22_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT20129.1"
                     /translation="MPRSARNTEEVDSVKQQILDAAVKIITNNGFSKLSMRRIAAQLG
                     VSATTIYNYYTSKDELYFYIRIKGFERLFEYFEKAYAIPVSTREKFIGIIHGYVDFGL
                     TYPDYYEIMFLNRNVPKFLDCVGTPLEAVATKEKAVALKPFIFTVEKLQHHLDIEPKL
                     AYELAMQLWVELNGLVSLCISRLLREVNEKQQRFVDSYLDTIVCRYLPVDM"
     gene            complement(2717..3616)
                     /locus_tag="PLX22_09290"
     CDS             complement(2717..3616)
                     /locus_tag="PLX22_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20130.1"
                     /translation="MVNDQIYYIYDNAVWCRSNNIESGYGYRLEGVAVPIDLYVYNHL
                     SKQKVYLLYNWGVLEYTEKRIYNIKNPVAVVRNHNTLYIATKDAIMVEIKPERFQLVY
                     EKGLSFGTITDIYVDPYFDDNRIETYEHQYNELEILADGKRIFLYNNDYIIEHYDSVS
                     FVQYGKNDRGNKTNEVLTVNDGLVFNSVYYKLNEPAYYPFHVYIKFDFNLQGVYFIAH
                     ENLYLLITNIYTNECAVALMAQGVAKVDGNRDYLVIFGTDNSVGFLKMDEVEAMIQKY
                     NFFPQIEWRTKLDLMIERSRSRL"
     gene            complement(3606..4022)
                     /locus_tag="PLX22_09295"
     CDS             complement(3606..4022)
                     /locus_tag="PLX22_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20131.1"
                     /translation="MPIEVKILGYVSFQKLKKPEHALIKYVPINSVIPIFNWQLYNKD
                     LNNQHTPYLLHRKKIHYRYYSPSKRFIILFGKILVIYMCTNVVLNTAKYLQKINNTPP
                     RNHFFENYINTDQRYNNNILKQLKQFKKKNGGVYGK"
     gene            complement(4026..4307)
                     /locus_tag="PLX22_09300"
     CDS             complement(4026..4307)
                     /locus_tag="PLX22_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20132.1"
                     /translation="MKVKLKFEDILVVIAYAQVLSMFYFIEDVNTTFIATIIITSWFV
                     AQKVKSHIRKKDVLYTIMHRAHSIKKIVSVDLHRLDGIYEAFKEDELGR"
     gene            complement(4360..5445)
                     /locus_tag="PLX22_09305"
     CDS             complement(4360..5445)
                     /locus_tag="PLX22_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20133.1"
                     /translation="MNTGMAHNIFSIIIPMLKAGTVAVTIALIMLFISKKLILVRQII
                     CIKRYAAKYQLAMGSITSRFAVNLPVVAESLQIPDEIIDEAVQNSKPSSDSWLYMPHQ
                     GHVYRIHIARDIEEIELENIGALQVHHIGDSRCKGEYAVLTQNGVTIYQCADDHLVLK
                     KHYPHDNIQQFYADSEIVITAAYKTIIIHNRKDTTTLRYSVDEGVCTYVSAKSLVVDS
                     VNDNKKYAVIYFGNTIYFLDLSARYIQFEPIRYITTNVPITGEYEKGFIMNEGFTYGN
                     VFIDIGTYPQKLQYAVFGGRNCVFFIAHEILYCIEVIGQDTLTGLGINIVSHQVDDFC
                     VADNVLVAHQIDNGFIRYFNYEEAEVA"
     gene            complement(5766..6326)
                     /locus_tag="PLX22_09310"
     CDS             complement(5766..6326)
                     /locus_tag="PLX22_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20134.1"
                     /translation="MNPHNHHRRSIRLRGYDYTQPGAYFITMCTHNRECLFGDIANGE
                     MVLNEWGKMVWDEWFKSQSIRNEITLYDNEFAVMPNHIHGIVWIVNTDNIGVNDVGAN
                     GRSPLQLPMTQPIGSPKRSLSSFVAGFKSAVTKQINQLRQTPGMPVWQRNYYEHIIRN
                     NNELHRIQQYIINNPKNWELDENYHI"
BASE COUNT         1866 a         1319 c         1105 g         2174 t
ORIGIN      
        1 gggtcagagc cttgatgata gcagcgtcgt tgtgcattga tggtgtagca gcggttatta
       61 aatttacctc gcataaacgc tttaaatagg tgtcactgtt aacacccggc atgtagcgcg
      121 cgttgacgtt gggatcacga actgagagca tttcagttac ctttaaaaag atgtaggtca
      181 tatcgcatac tgcatcggtg ccctcaggcg ttactccgcc cagtgtaata gcctggtcgg
      241 aagaactgcc gccaaagtag aagttagcta aatcgggggt aagcggaaaa tggtcggtac
      301 agcgcatgta aaaatcacca ataagttcaa gggcaaattt catgtattca tcacgctttt
      361 caggcgggat gtttttcata tcgtttacga agtacggttg cagccattga tcaagcctac
      421 ccaatgaaag ccctgcattc atactctcca tgtgcagtgc aatccatata atccatactg
      481 ccataactgc ttcatgcagc gtttgtgctc catgcatggg tatccgactg caaatatccg
      541 ctattgtcaa aagttcttgt ttacgctgag gattctgttc ccggttacta tcgcccatag
      601 cctgttttga aaggttttgt gcatacgcat tgacaccttc cagacacaaa atcattgccg
      661 ttaaacaatt ttgtttttcc tcaccacacc cgctatttag ctcttcatgc atttcctgta
      721 tcattcccag agtacccata gccaaaactt ttggaaagtt tggaatagta tgtgatatgg
      781 ttgcctgctt ccagttgaaa tacagcgcat atcgttcatc taactgctga ctcaacggat
      841 tattatattt gtcgcgcacc cactctctga aattgcggtg cagccagaaa ggaaaaacat
      901 caaagtgtaa aacttcacgt gtttgttcat caatatcaaa cggattgtag gtacggtatg
      961 atatcgtcat gagttcgttc catatcataa tgccgcttcc atcaggatat atggggcagc
     1021 caatgggctt gctggtggta gttcccgcta aaagcgactc atcgtttata agcggctttc
     1081 ggttttccat catgtatttg tacgccaatg cgttgcgcac aatggggtcc caattgttac
     1141 cggatgaatc cttctcaaat ccattttccc ggtaccattt tgttaaaaga tatgcacgct
     1201 cagcgcacac tgctggtttt tttgtcagat gatcatgtag aaatcgttgt aaacgcggga
     1261 agtcatccat gctatactta gacaaatagg catcatcaag atacacaacg tgcgggtcgg
     1321 ctccctcagc tttcagacgc tcctttctac gctttacgat ttcatcgctt aactgtttat
     1381 tgtgctctgg ttgttgccgt ttaactgttt tatcatcttt tttgcgctca gttttcattg
     1441 cacgtttttg tacatcatga aacagcaatg aaatgaaaaa cgaaaaaata tttgcatagc
     1501 ttatgcttcc ttctattcgc acatcgcttt ttaatagcat gtagatgaca tcggttggcg
     1561 tacctaagag cagcttgcgt gccgcttcat catttttaaa aatcataaca gctttcgcat
     1621 catgggcgat agtttttgta acagcaactt tcccctcaaa aaaagaaatc ccatactgta
     1681 aagaattatc ttcggttttc agaccaatgc taaaattaag ccatccgtct ttagatgtaa
     1741 gatagcggtt gagggattta cgattattaa aattgaaagc aatgaatttt aaaaataaga
     1801 aaattattat tctggtaccc ttgcttgatc tgatatttgt tgtatacagc aacatggcaa
     1861 acctcctgat aatgactaaa gtataacctc acccccaacc cctctccttc gcaaggcaag
     1921 ggggagttat tcctaacact gcatttacat atcaaccggc aaataacggc atacaatggt
     1981 atccagataa ctatcgacaa aacgctgctg cttttcattt acttctctga ggagcctgct
     2041 gatgcacaaa cttaccagtc cgtttaactc cacccatagt tgcattgcca attcatacgc
     2101 cagctttggt tcaatgtcca gatgatgctg taacttttct actgtaaata tgaaaggctt
     2161 caatgccact gctttttctt tagttgcaac agcttcaagt ggcgtaccca cacaatccag
     2221 aaactttggc acatttcggt ttaaaaacat tatttcataa taatcaggat aggttaaccc
     2281 aaaatcaaca tagccatgta taatacctat aaacttttca cgggtagaaa ccggtatggc
     2341 atatgctttt tcaaaatatt caaacagccg ttcaaagccc ttaatgcgta tataaaagta
     2401 tagttcgtcc ttactggtat aatagttgta gatggttgta gcactgaccc ctaactgtgc
     2461 tgcaatcctc cgcatgctga gttttgaaaa tccattgtta gtgataatct taacggcagc
     2521 atcaagtatc tgctgtttaa cactatcaac ttcttctgtg ttgcgtgcac ttcgtggcat
     2581 agtttttggc ctctaatata ttatcagtgt tattttaaca gtgttaacct tatttgccaa
     2641 gcaaaaataa aattttttcg atagttattt tcaaataagg ctattaaact tacagtaaat
     2701 acaggggtaa atgtgcttaa agtcttgatc gagagcgttc tatcatcaaa tctaactttg
     2761 ttctccattc tatttgcgga aaaaaattat atttttgtat cattgcttca acttcatcca
     2821 tttttaaaaa accaacggaa ttatctgtcc caaaaataac caggtaatcc ctattaccat
     2881 ccactttcgc tactccctgc gccatcaatg caacagcaca ctcattggta tatatattag
     2941 ttatgagcaa atacaaattt tcgtgtgcaa taaagtacac accctgaaga ttaaaatcaa
     3001 atttgatata aacatgaaaa ggataataag caggttcatt gagtttatag tacacgctgt
     3061 tgaatacaag cccatcattg actgttaata cttcatttgt tttgttacca cgatcgtttt
     3121 ttccatattg tacaaaacta acgctgtcat aatgttctat tatataatcg ttgttatata
     3181 aaaatatgcg cttcccatca gctaaaattt caagttcatt gtactgatgt tcgtatgttt
     3241 ctatcctatt gtcatcaaaa tatggatcta catatatgtc ggttattgtc ccaaacgata
     3301 aacctttctc atataccagc tgaaatcgtt ccggttttat ctctaccatg attgcatcct
     3361 tcgttgcaat ataaagtgtg ttgtgattac gtactacggc aacaggattt tttatattgt
     3421 atattcgttt ttcggtatat tcaagcacac cccaattgta cagtaaatac actttttgct
     3481 tactcaagtg attatatacg tacaagtcaa ttggaacagc tacaccttcc agcctgtatc
     3541 catacccact ttcaatatta ttacttctac accatactgc attatcgtaa atataatata
     3601 tttgatcatt taccatatac accaccgttt ttctttttaa attgtttaag ttgttttaaa
     3661 atgttattgt tataccgttg atcggtattt atataatttt cgaaaaaatg attccgaggg
     3721 ggtgtattat taatcttctg gagatatttt gcagtattta acacaacatt tgtgcacatg
     3781 taaattacaa gaatcttacc aaaaagtata ataaaccgtt ttgacggaga ataataccgg
     3841 taatgtattt ttttccgatg taaaaggtat ggtgtatgtt gattgtttaa atctttatta
     3901 tacaactgcc agttgaatat gggaataaca gaattaattg gaacatactt tattaaagca
     3961 tgttcaggtt tttttaattt ttgaaagcta acatatccca gtatttttac ctcaataggc
     4021 atagtttacc tgcctagttc atcttcctta aatgcttcat aaataccatc aagcctgtgt
     4081 aaatctacgc taacaatctt tttaattgaa tgagctctgt gcataatggt gtacagaaca
     4141 tctttttttc gtatatggct ttttaccttt tgtgcaacaa accatgaggt aataataatt
     4201 gttgctataa aagttgtgtt aacatcttca atgaaataaa acattgataa cacctgtgca
     4261 taggcaataa cgaccagtat atcttcaaat tttagtttta ctttcatgct ccctcctgta
     4321 ttgtacgctt acctatattg catagtaatc attatgtgct tatgctacct ctgcttcttc
     4381 ataattaaaa tatcgaataa atccattatc aatctggtga gcaactaaaa cattatcagc
     4441 aacacaaaag tcatcaacct gatgggatac aatatttatg ccaagacctg ttaatgtgtc
     4501 ctgccctata acctcgatgc aatacagaat ttcatgcgct atgaagaata cacaattcct
     4561 gccaccaaaa acagcatact ggagcttttg tggatatgtc ccaatatcga taaaaacatt
     4621 tccgtaggta aatccttcgt tcattatgaa tcctttttca tattcgcctg taataggtac
     4681 atttgttgta atgtaacgga ttggttcaaa ctgaatatat ctagctgata aatccaaaaa
     4741 gtatatggta tttccaaaat agattactgc atattttttg ttatcattta cgctatcgac
     4801 aactaacgat tttgctgaca catacgtaca gactccttca tcaacactgt accgtaatgt
     4861 agttgtatct ttcctgttat gtatgattat tgttttatac gctgctgtta ttactatctc
     4921 actatctgca taaaattgtt gtatgttatc atgaggataa tgtttcttta gcactaaatg
     4981 atcatcagca cactggtata tagtaacgcc gttttgtgtt agcacagcat attccccttt
     5041 acagcgacta tcgccaatat gatgtacctg aagcgcgcct atattctcca gttcaatttc
     5101 ctcaatatca cgcgcaatat gtattctata cacatgtccc tgatggggca tatacagcca
     5161 gctatcgctt gatggcttac tattttgcac agcctcatca attatttcat ctggtatctg
     5221 taacgattcc gctacaaccg gcaaattaac agcaaaccgg ctggtaatag aacccattgc
     5281 caactggtat ttagcagcat accgttttat acaaattatt tgtctcacca aaattagctt
     5341 tttactaata aaaagcataa tcagggcgat agttaccgct actgtacctg cctttaacat
     5401 gggaattatt attgaaaaaa tattatgtgc cataccagtg ttcatatagc cccctttttc
     5461 atattcactt tattgtgtat attagactat tttttccttc attatatagg gaatatactt
     5521 cctggtgaat gacatataat gtccctggtg aaattttttt tattttttat tgcgatattt
     5581 ttttatgcgt actattgaga atatccaacc aatattggtg tgcaattttc cccatttcat
     5641 ttaacaccat ttcgccattt gcaatgtcac caaacaaaca ttcccggttg agggtacaaa
     5701 tggttataaa atatgcaccg ggttgtgtat aatcacatcc cggtaaacgg atggaacggc
     5761 ggtgattata tgtgataatt ttcatccagt tcccaatttt tagggttgtt tataatatat
     5821 tgttggatgc gatgtaattc attgttgtta cggatgatgt gttcataata attgcgttgc
     5881 cagacgggca taccgggtgt ttggcgcaat tgattgattt gtttggttac ggcggattta
     5941 aaccccgcaa caaatgacga caatgaacgt tttggggatc caatgggttg cgtcatcggc
     6001 aattgtaggg gcgaacggcc gttcgcccct acatcattca ccccaatatt gtcggtattt
     6061 acaatccaca caatcccgtg gatatgattg ggcattacgg caaattcatt atcatataat
     6121 gtaatttcat ttcgtattga ttgggattta aaccattcat cccataccat tttcccccat
     6181 tcatttaaca ccatttcgcc atttgcaatg tcaccaaata aacattcccg gttgtgggtg
     6241 cacatggtta taaaatatgc accgggttgg gtgtaatcgt atccccgcaa acggattgaa
     6301 cggcggtggt gattgtgcgg attcataatt gtatttgtta ttatattttt ttatgttaca
     6361 tttccttcca ccacctgtat ctcttcatcc gttaaatcat acaacttgta aaccagtgta
     6421 tctatttccc gttcccaccg ggttgtatcg gcgcttgggt tttg
//
LOCUS       DAOSOX010000136         6463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12065_length_6463_cov_8.441011, whole genome shotgun sequence.
ACCESSION   DAOSOX010000136 DAOSOX010000000
VERSION     DAOSOX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12065_length_6463_cov_8.441011"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1448
                     /locus_tag="PLX22_09315"
     CDS             246..1448
                     /locus_tag="PLX22_09315"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT20135.1"
                     /translation="MNILDLRPENFKKAFPDSKVMHVNGKSIIKSAMKEGNHWVAMAV
                     NTRHEIAIPGIIKASMELNAPVIYELALSESDLSGGYTGLTPADFIKLVVSLNDKMGN
                     AGEKAVPFTCHRDHTTVQEVSRKAFDKADAIIKASIEAGYGSFSIDVSFLPMEQNIAL
                     SILLSEQVIRAGLMYESEVGEIGGAHGNSSVSEAVTLVEALTKLGQCPDLIAINNGTT
                     HGNVRGNIDLQLTHNTYRAMSPWGVGIAQHGTTGTSIEDVGQFYRYGIFKANVGTLWQ
                     NVIWGIDMDEYGVAVEKGPTYEKDPDKGISMELWKKMEEYALANNMKGGNMKKLFKPF
                     KDAMNDEYYNNAKIRDRVDKAVYETAAAFFKALKAVGKGDDVKAQMETITTPSKQGSK
                     ESKERDYE"
     gene            1441..3069
                     /locus_tag="PLX22_09320"
     CDS             1441..3069
                     /locus_tag="PLX22_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOT20136.1"
                     /translation="MSKPITKSWWKHGVLYQIYPRSFKDSNNDGIGDLEGIIQSLDYL
                     QDVGVDGIWLSPIYRSPMYDFGYDVSDYCSIDPIFGTIANFKKLLKEAEKRNIAIIMD
                     MVLNHTSHLHPWFVESRSSVDNPKRDWYIWHPGKNGKVPNNWMAAFGGRAWTWDELTR
                     HYYLHLFTEQQPDLNWHNPEVKKAMVDVLRYWLDMGVKGFRFDVINYIVKDINVRSNP
                     YRLRLTYPRRHDLQVHIYDRNQPEIHDILKELRQLLDSYGDTMSVGETYPNEGEKEPA
                     MAASYLGNNDELHLSFDFSTIYARFNAREFERILKNWYHAIGDSGWPCHVLSNHDQSR
                     AITRLANNDVQKAKLLAVMLLTQKGTPFIYYGEEIGMVDGKIKRSQIQDPLGKKYWPF
                     HKGRDKARTPMQWDMTQNAGFSNGKPWLPVNSDYVTTNVLQQQGNKQSILHLYKDCIA
                     IRRLHPALYAGDIEFVPAPDDVLLYKRCHNNEQLMIALNFSKAKKDVTIGLEGEVLLS
                     SCGNSSIHSSHSSHSSIHGGALTLDAYEAVIIKV"
     gene            complement(3178..5598)
                     /locus_tag="PLX22_09325"
     CDS             complement(3178..5598)
                     /locus_tag="PLX22_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOT20137.1"
                     /translation="MENMKSSNTTETQIISHDEAHMLEWDTLISFIANLCYTQPGKNY
                     CMGMQPLDYNEILEQYAKINCLKEILSIGHHPDFSGIHDITYLLEKSSKGATLQLDEI
                     FKIRLFITAQYRITSFFKSIQYPHNALTELQMLHPCEQLYTMLISSLTDSGNLNENTY
                     PVLKKLQREINDIRNAIEKSLQNLIHSHEYQQIVQEKIFTVKNNRYCIPIKTTFKNKL
                     KGTIADISSSGATLYIEPDSIHDLNNTLLYKELDLQREIEKILQILSSTIAEHAHLLM
                     ENAAIIGYLDFCTALARFAVTYNASTPALSRQPYLRLFNAKHPLLVTMHGKKIVPCDI
                     ECGINFKCLIISGVNTGGKTVLLKMLGLFALMVRYGLPITASPDSTAGYFESVYADIG
                     DEQNIMQSLSTFSGQIKNIVRILNLANEKSLVLIDEIIVGTDPKQGAAIAQAVLQYLV
                     NKKAVVAVTTHYSQLKEIASVDSQFENASVVFDIHTMQPTYQVLMGIPGASYTIEIAK
                     NLSLPQKIIDDAISLLDSTSLTVDALLSQITQQSHTLHQKEQELALLKQELNQLKNDY
                     DKKINELQQQIEKVTKEQAIEFVHELHQYRNTIIDKIRTMHSMSMKDATQLVNDITQM
                     QLSLQDTIKQLQKPSEDFLPCTVDTVQKGSKVFIKPLEQYGYVEDVDEKSKQLKVRMG
                     TVNSRFSFDDVYCIPESKKLTTVKTKTNTIATQDIQHIPMSIQTKYNTIDLRGLYVDE
                     ALQKMDHELDNLLKNNIPTAIIIHGHGTGSLKKAIRQYLKHSFYVKGYRPGQPEEGGD
                     GVTVVQLR"
     gene            complement(5673..5945)
                     /locus_tag="PLX22_09330"
     CDS             complement(5673..5945)
                     /locus_tag="PLX22_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT20138.1"
                     /translation="MNGDDPINIALTDTIDLHVFHPADITAILDEFLNNAVLQGYLQV
                     EIIHGKGKSVVKHTVHNYLKNDKRVLQFFDKSANWGRTIVILKKEV"
     gene            complement(6136..>6463)
                     /locus_tag="PLX22_09335"
     CDS             complement(6136..>6463)
                     /locus_tag="PLX22_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20139.1"
                     /translation="TVTIAHKNYNGVRACITFVGRQKLYPFVRGYLCAKYPLTNPVEP
                     PQTAVCLIPLYGLYLCHRAAEDILSLSSHEPYGATASMPSLFFHFYSVGITFCNRTFT
                     IHFKIS"
BASE COUNT         1953 a         1130 c         1360 g         2020 t
ORIGIN      
        1 gcgttggaag tatgattttt tagaattttt atttttgttc agaggcttca attatattac
       61 aaaaattcat aatatagtaa ataattatgt aataataggg taactccatg tatagctata
      121 acttgttata tgatatagca tacaatcttt tgcaaaaatg taattatcac ggcaaatttt
      181 acttgaataa ataattgata gtattacggt attagcaaat attacaatac acatcagggg
      241 gaactatgaa tattcttgat ttacgaccag aaaactttaa aaaagcattt cccgattcaa
      301 aagtgatgca tgtcaatggc aaatcaatca taaaatccgc aatgaaggaa ggaaaccact
      361 gggtagctat ggctgtcaat acacgccatg aaattgctat tccagggatt ataaaggcat
      421 cgatggagtt gaatgcaccg gttatatatg aactggctct ttcagaaagc gacctgtcag
      481 gtggctatac agggcttaca cctgcagatt ttattaaact ggttgtcagc ttaaatgaca
      541 agatgggcaa tgcaggtgaa aaggcagtgc cgtttacatg ccacagagac cataccactg
      601 ttcaggaggt gagccgtaag gcgtttgata aagccgatgc aataataaaa gcttcaatag
      661 aggcaggata tggttcattt tcaattgatg tttcattttt gccgatggaa cagaatattg
      721 cattgagcat attgctaagt gagcaggtta tacgagctgg tttaatgtat gaatccgagg
      781 ttggcgagat tggaggagct catggcaatt cttcggtaag tgaagcagtg acacttgtag
      841 aggcattgac aaaattgggg caatgccctg atttaattgc catcaataat ggaacaactc
      901 atggcaatgt tcgtggtaat attgatctac aattaactca caatacctac agggctatgt
      961 caccatgggg tgttggtatt gcacagcatg gcaccaccgg tacatccatt gaagacgttg
     1021 ggcagtttta caggtatggc atttttaaag ctaatgttgg tacgctgtgg cagaacgtta
     1081 tctgggggat agatatggat gaatatggag tagcagttga aaaaggtcct acgtacgaaa
     1141 aagaccctga taaaggcata tccatggaac tgtggaaaaa gatggaagag tatgccctgg
     1201 caaataacat gaagggcggc aatatgaaaa agctcttcaa accgtttaaa gatgcaatga
     1261 atgatgaata ttataacaat gcaaagatac gcgatcgtgt tgataaggca gtatatgaaa
     1321 ccgctgctgc attttttaaa gcgttaaagg cagtaggcaa gggggatgat gtaaaagctc
     1381 agatggaaac tataactaca ccatctaagc aaggttcaaa agaatcaaaa gaaagagatt
     1441 atgagtaaac ccataacaaa gagctggtgg aaacacggtg tactgtatca aatatatcca
     1501 cgcagcttta aggattcaaa taatgatggc attggcgatc tggagggtat catccaatcg
     1561 cttgattatc ttcaggatgt aggtgttgat ggtatatggc tatcgcctat ttaccgctca
     1621 ccaatgtatg actttggtta cgatgtgagt gactattgtt caatagatcc gatatttgga
     1681 actatcgcca attttaaaaa attattaaaa gaagccgaaa aacgcaacat tgctatcatc
     1741 atggatatgg tgttgaacca tacatcgcac ctgcatccgt ggtttgtgga atcacgttca
     1801 tcagttgata atccaaagcg tgattggtat atatggcacc ccggaaaaaa tggtaaagtg
     1861 cccaataact ggatggctgc attcggcggt agagcatgga cgtgggatga gttgacgcgt
     1921 cactactatc ttcatctttt taccgagcag cagcctgatt tgaactggca taatcctgaa
     1981 gttaaaaaag caatggttga tgtactgcgc tactggcttg acatgggtgt aaagggtttt
     2041 cgatttgatg taatcaacta tattgttaag gatattaatg tacgaagcaa tccctatagg
     2101 ttacgtttaa cctatccacg acgtcatgat ttgcaggttc atatatatga tcgcaatcag
     2161 cctgagatac atgatatcct gaaagagtta cgtcaattgt tagatagtta tggtgatact
     2221 atgagcgttg gtgaaacata tccaaacgaa ggtgaaaaag agccagcgat ggctgcatcg
     2281 tacctgggta ataatgatga gttgcatctg tcatttgatt tttcaactat ctacgcacgc
     2341 tttaacgcac gcgagtttga acgtatctta aaaaattggt accatgctat tggcgatagt
     2401 ggctggccat gtcatgtact gtccaatcat gaccagtcgc gagcaataac acggctggca
     2461 aataatgatg tgcagaaggc aaagctgtta gcagtgatgc tgttaaccca gaagggaacg
     2521 ccatttattt attacggcga agagataggt atggttgatg ggaagattaa acgcagccag
     2581 atacaggacc cgctgggaaa gaagtactgg ccatttcaca aaggcaggga taaagcacgt
     2641 actcccatgc aatgggatat gactcagaat gcaggctttt caaacggtaa accgtggctg
     2701 ccggtcaata gtgattatgt aaccactaat gttttgcagc agcagggtaa caagcagtca
     2761 atattacacc tgtataagga ttgtattgcc atcagaagat tgcatcctgc tctgtatgcg
     2821 ggtgatattg aatttgtacc tgcaccggat gatgtgctgc tgtataaacg atgccataac
     2881 aatgaacagt tgatgattgc attgaatttt tcaaaagcaa aaaaggatgt aaccattggg
     2941 ttagaaggcg aggtgttgct ttcatcctgt ggaaattctt caattcattc ttcgcattct
     3001 tcacattctt caatacatgg gggtgctctc acgcttgatg cgtatgaggc ggtgattatc
     3061 aaagtataat aaatataata agtacaataa aagagtagaa tattagtaca tgcatgcagt
     3121 cattcattac atttattaat agtacatact gttgttgttg tattgtttat gaaaagctca
     3181 tcgcaactgc actactgtta cgccatcacc accttcctcc ggctgtccgg gacggtagcc
     3241 ttttacataa aacgaatgct taaggtactg gcgaatagct ttttttaaag agcctgtccc
     3301 atgtccatga ataataatag cggttgggat attgtttttt aaaagattat ccagttcatg
     3361 atccattttt tgcaatgctt catcaacata taaaccacga agatcaatgg tattatattt
     3421 tgtctgaatg ctcattggta tatgctggat atcctgtgtt gctattgtat tggtttttgt
     3481 tttaacagtg gttagctttt tgctttccgg aatacaatac acatcatcaa atgaaaacct
     3541 gctattcaca gtacccatgc gtactttcaa ctgcttgctt ttttcatcaa catcttcaac
     3601 atatccgtat tgttccagtg gctttataaa tacttttgaa cctttttgca ctgtatcaac
     3661 agtacagggt aaaaaatctt cagagggttt ctggagctgt ttgatagtat cctgcaatga
     3721 aagctgcatt tgtgtaatgt catttacaag ctgtgttgca tccttcatgg acattgaatg
     3781 catggtgcgt attttgtcaa tgatggtgtt tcggtattga tgaagttcat gtacaaattc
     3841 aattgcctgc tcttttgtta ctttttctat ttgttgctgt agctcattta tctttttatc
     3901 gtagtcattt tttaattgat taagctcctg ttttaatagt gcaagttcct gttctttttg
     3961 atgtaatgta tgcgattgct gggttatttg ggaaagcagt gcgtccacgg ttagcgaggt
     4021 gctgtcaagt aaggatatgg catcatcaat gattttttgc ggtaaagaca aattttttgc
     4081 tatttcaata gtatagcttg ccccaggaat acccatgagc acctgatagg tgggctgcat
     4141 ggtatggata tcaaaaacaa cggatgcatt ttcaaattgg ctatcaacag aagcaatttc
     4201 ttttagctgt gaataatgtg ttgtaacggc tacaaccgct tttttattta ctaaatactg
     4261 caacactgct tgtgctattg cagctccctg ttttgggtct gtacctacaa taatctcatc
     4321 aataagcacc aggctttttt cattagcaag gtttagtatg cgtacaatat tttttatctg
     4381 tccggaaaag gttgacagcg actgcataat attctgttca tcaccaatgt cagcatacac
     4441 tgattcaaaa tatcctgctg ttgaatcagg gctggcggtg atagggagtc cgtatcgcac
     4501 cataagagca aaaagcccta acatttttaa aagcactgtt ttcccgccgg tgtttacgcc
     4561 cgatatgata agacatttaa aattgatgcc gcattcaata tcgcatggta caattttttt
     4621 gccatgcatt gtcacaagca aaggatgttt tgcattaaac aaccgcagat atggttgccg
     4681 ggaaagtgca ggcgtggatg cattgtaggt tactgcaaag cgggcaagtg cagtgcaaaa
     4741 atctaaatag cctattatgg ctgcattttc cattagcaaa tgagcatgtt cagcaattgt
     4801 ggacgatagt atttgcaata ttttttctat ttcgcgttga agatctaatt ccttatataa
     4861 cagggtgtta ttcaagtcat gaatgctgtc aggttcaata tacagtgttg cacctgaaga
     4921 tgatatatct gcgatagttc cttttaattt gtttttaaat gttgttttaa tgggtataca
     4981 gtatcgattg ttttttaccg tgaaaatttt ttcctgaaca atctgctggt actcatgcga
     5041 atgaataaga ttttgcaatg acttttcaat tgcattgcgt atatcattaa tctctcgttg
     5101 cagtttttta agtaccggat aggtattttc attaagattt cctgaatccg ttaatgaaga
     5161 aatgagcatg gtatatagct gttcacaggg atggagcatc tgcaattcgg ttagcgcatt
     5221 gtgtggatac tggattgatt taaaaaaaga agtaattcta tattgtgcag tgataaacaa
     5281 tcgtatttta aaaatctcat caagctgtaa agttgctcct tttgatgatt tttccagcaa
     5341 gtacgtaata tcatgtatgc ccgaaaaatc gggatgatgc cctatggaca atatttcttt
     5401 aagacaatta atcttagcat attgctctag tatctcatta taatcaagtg gttgcatccc
     5461 catgcaatag tttttgcctg gctgagtata acataaattg gcaataaatg atataagggt
     5521 atcccattcc agcatgtgtg cttcatcatg agatataatc tgcgtttcag tggtattact
     5581 gcttttcata ttttccataa cttcctaata gtattgtacc aaaacattat tgtaaagagt
     5641 aattattgca aagcaccttt aatgcaactg cattaaacct cttttttaag tattacgata
     5701 gtccttcccc aattagcgga tttatcaaaa aattgcaaaa ctcgtttatc attttttaag
     5761 tagttgtgga cagtgtgttt tacaaccgat ttcccttttc catgaattat ttcaacctgc
     5821 aaatacccct gtagtacagc attgttcaaa aattcatcta aaattgctgt aatgtctgca
     5881 ggatgaaaca catgcagatc aatagtatcg gttaatgcaa tattaatagg gtcatcacca
     5941 ttcataagcg gttgttactc agctattggt tccagaaatc catcatccgt tatcaggaat
     6001 gctccataca gtcaataatg cgttacgaag ctgttttcaa tatacacaat acgccatgtt
     6061 ttgtaaagta cattgtcatg gcaacgatag ttgttttgaa aaagttttaa gctctgaccc
     6121 cattttttgt cacacctatg agattttgaa gtgaatagtg aatgttctgt tacaaaaagt
     6181 gatccctact gagtaaaaat gaaaaaacag cgaaggcatg gatgccgtag cgccatatgg
     6241 ctcgtgcgag gacaaagaca ggatgtcttc cgcagcacga tggcaaagat ataatccata
     6301 cagcgggata aggcacaccg ccgtttgagg tggctccaca gggttggtaa gggggtattt
     6361 agcgcataaa tatcccctta caaaaggata taacttttgt cgcccaacaa aagttataca
     6421 tgctctgacc ccattgtaat ttttatgggc gatagttacg gta
//
LOCUS       DAOSOX010000137         6433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12152_length_6433_cov_5.746159, whole genome shotgun sequence.
ACCESSION   DAOSOX010000137 DAOSOX010000000
VERSION     DAOSOX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12152_length_6433_cov_5.746159"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLX22_09340"
     CDS             complement(<1..377)
                     /locus_tag="PLX22_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20140.1"
                     /translation="MHITITRKNSINVISLALLLPALLMSLQCNKGNDILATYNGGAV
                     TRQEFMDWLELNYYKREVSHIMKYKTLRIQKVQEMCKEKILILEAKKQGYDKQPYFSN
                     IKELYADQEIMKYFLKKEIEEKA"
     gene            complement(418..5586)
                     /locus_tag="PLX22_09345"
     CDS             complement(418..5586)
                     /locus_tag="PLX22_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20141.1"
                     /translation="MEKILGMQIIQKIAESPRTITYRIEYKDSTAILKVFNIKQYSLS
                     DFIRFKQEYEIIMNPDISGIVKPYEFIDTHEGFGIVVEDFDSISLKDLLMKKKIVALN
                     EFFTIAIKVSQIVGTFHQYNIVHQDIKPSNILLTEDLHTIKITDFGIRTLFPNEYQEI
                     YRKDIMEGTLLYMSPEQTGRLNRNIDYRTDIYSMGITFYELLTGNVPFKSDDPIEIIY
                     AHIARKPVPVSEVNPHIPGIVSQIIDKMLAKTPEERYQNSFGIVGDLIQCNDEYTKKG
                     TISPFKIASFDIPLRFNIPQQIVGRDSEIQSLLSSYKNFMEGHREIVIVKGSPGIGKS
                     ALVNEIYKPITATKGYFISGKYDQFKRDIPYSSIIQSFQMLIRQILTEDLSKLELWKE
                     ELLNAFGKSGKILTDVIPDLEHIIGPQLPVEQLSPEESQNRFNYLFKKFINVFAVQEH
                     PVVLFLDDVQWIDLASLNLIRSIVLDESVKNFFLIMAYRDNEIDASHPINPVMHEIKD
                     NGVTINIIALAPLKEEDVNTFLLNFLSNNVESTMELSSLIYSKTAGNPFFIIQFLKRL
                     FEDKLITFSPEKGWIWNIEKVKELPITDNVVDMLSQSINKMPAENIELLTICACIGNR
                     FELEDIIAITGETAEAILKVMNSLIEFGFIGLFKNIYRFYHDRIQEAAYSLLDEEERS
                     KLHYKIGKNSLSKTAKENIQDKIFYIVYHLNKSVHLIQTVEERIQLAELNLIAGEKAK
                     NSSAFDSSLIYFKQAAELLPENAWIDYYRLTFKIYLNYAESEYLAGDQNNAATLFDVL
                     SHYAQSRLDKAEIYINRCLFLTNGGQYEEAVRLGLEGLKMFGISIPEKPSMLNVIKVM
                     LALKIKLQFIKPEDLLQLPWIENPEEIAVIRIFESVTQAGYYTSRNLVIIGPIILFNF
                     IIKTGNCYLSSYLFSVLAGIVGPGLGDYKWANQFYQLSMKLLDKTPEKNACSVYFTLA
                     YLYLHWNAHVQENIVLFRKSYNMFLGLGKFDFAAHSINNIADYSFILGKNLDEIFTEY
                     KNYEKFFKNQKDKFFHSYYEDNIRKYYCYKGLTDSPSTYNIHDNYTEERLQAVRQTKN
                     NLDKFIFVFNLMKIEFLYRNLQKGYEYAREIEPIKNELVGNIHYPEFVLYYLLLLAGL
                     RKDKKISKIIWAMKSKLLVSMMKKWSTVCPFNYLHKYYLMLAEKYAVTKNYKKAKEYY
                     DKAISEANRNGYIFIEAIAHECLGSMYISLNDKTNASQQLEKAYNLFHQCGADAKATM
                     LHNNYRDKIKIEQTRTAIAIDSDSSSIASSLDIVDFSSALKASLAIAGELNFEKLPGK
                     LLSIMIENAGAEKGFLILKDNEDLIVVARKNTIDEEITALENLPVDRVDGFSHAIVRL
                     VARTGEKVIINDAMNDPFYVNDEHIFKNKVKSLLCLPILNQGKVVAVLYLENNVITAA
                     FTQRHIALLTLISSQAAISIENAQLYLNLEKKVEERTKELKEARDALWGEMQLAAKIQ
                     TVLLPKSPMAKGYEIYGYMIPATEVGGDYYDVINTSTNDWIIIGDVSGHGVSAGLVMM
                     MVQTAIQTIIRANPNLTPVTLLKVVNRTITYNIKQMNEDKYMTITALKLYPEGKVEYS
                     GLHQYILVYRYATKQVDRMESDGLWLGFDIDLNRDTSLKEFTMNKGDVFLLYTDGITE
                     AKTDNGEMFEEDQLANLLQNSGEKPLAEIKNDIIAALRNYHTNDDVTMVLTRKV"
     gene            5984..>6433
                     /locus_tag="PLX22_09350"
     CDS             5984..>6433
                     /locus_tag="PLX22_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017340186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOT20142.1"
                     /translation="MSYPLHDMKILDFTYLLPGPYGSMMLADLGADIIKVENPHNPDM
                     LRMLPPIVDGISAVYAHLNRGKKSLALDLKAPDANNIIYKLVKEYDIVIEQFRPGVLE
                     KLGFGYEDLKNINQGIIYCSLTGYGHTGSYASRAGHDINYMALSGVES"
BASE COUNT         2009 a         1170 c          956 g         2298 t
ORIGIN      
        1 ttagcttttt cttctatctc tttttttaaa aaatatttca ttatttcctg atcggcatat
       61 agctctttaa tattggaaaa ataaggctgt ttatcatagc cctgtttttt tgcttccaag
      121 atcaatatct tttctttgca catttcctgc actttttgta ttcgcaatgt tttgtacttc
      181 attatatggc taacttcacg cttataataa ttcagctcca accaatccat aaattcctgt
      241 cttgttacag cacctccatt atatgttgcc agtatatcat tgcccttgtt acactgcaaa
      301 gacatcaata gtgcaggtaa caataaagct aaacttatta cgttaatact attttttctg
      361 gttatagtta tatgcatata ctgcaacacc tccattccag tagtaataaa aacatactca
      421 taccttccgt gtaagaacca tggtcacatc atcattggta tgataatttc ttaatgctgc
      481 aataatatca ttttttatct ccgcaagtgg tttttcccct gaattttgta aaaggttagc
      541 caattgatct tcttcaaaca tttctccgtt atcagttttt gcttcagtaa taccatcagt
      601 gtaaagaaga aaaacatctc ctttattcat agtaaattct tttaaggaag tatcacggtt
      661 taaatcgata tcaaatccca gccataatcc atcagattcc attctatcta cctgtttcgt
      721 tgcatatcta tatacaagaa tatattgatg aaggcctgaa tattcaacct ttccttcagg
      781 atacagtttt aacgcagtaa tggtcatgta cttatcttca ttcatttgct taatattata
      841 ggtaattgtt ctgttaacaa ccttaagcaa ggtaaccggt gttaaatttg gatttgcgcg
      901 tataatagtt tgtatagccg tttgcaccat catcattacc aatcctgccg aaacaccatg
      961 ccctgataca tcaccaatga taatccaatc atttgtactg gtattaataa catcataata
     1021 gtctccacct acctcagttg ccggtatcat gtaaccataa atttcatatc cttttgccat
     1081 aggcgatttt ggtaataaaa cagtctggat tttagcagca agttgcattt caccccataa
     1141 agcatctcta gcctctttta gttcttttgt tcgctcctct accttttttt caagatttaa
     1201 gtaaagctga gcattttcaa tggagatagc agcttgcgaa gatatcaaag ttaacaatgc
     1261 tatatgtcgt tgtgtaaatg ctgctgttat tacattgttt tcaagataca aaacagcaac
     1321 caccttacct tgattgagta ttggtaaaca caacaatgac tttaccttat ttttaaaaat
     1381 atgttcgtca ttgacataga aaggatcatt catcgcatca ttaatgatta ctttttcacc
     1441 tgttcgtgcc actaatcgta ctattgcatg cgaaaagcca tctaccctgt ctactggaag
     1501 attttccaga gctgttattt cctcatctat tgtatttttc ctggcaacaa caatcaggtc
     1561 ttcattatcc ttgagtatta aaaagccttt ctcggcacct gcattctcta tcattattga
     1621 cagcaacttc cccggaagct tttcaaaatt aagttcaccg gcaattgcca gcgaagcctt
     1681 taatgccgaa gaaaaatcta ctatatccag tgaacttgct atagatgaac tatcgctatc
     1741 aattgctatt gctgttcggg tttgttcaat ctttattttg tcacgatagt tattgtgtaa
     1801 catggttgct tttgcatctg caccacattg atggaaaagg ttatatgcct tctcaagctg
     1861 ttgtgaagca tttgttttat cgtttaatga tatatacatg ctaccaagac attcatgcgc
     1921 aattgcctca atgaatatgt atccatttct attagcttct gagattgctt tatcataata
     1981 ttcttttgct tttttataat ttttggtaac tgcatacttt tccgccagca taagataata
     2041 tttatgcaga taattaaaag ggcaaactgt tgaccacttt ttcatcattg atacaagtag
     2101 ttttgatttc attgcccata ttatttttga aattttttta tccttcctaa gccctgctaa
     2161 tagcaataag tagtataaaa caaattcagg ataatgaata ttcccaacca gttcattttt
     2221 tattggctca atctcccggg catattcata tcccttttgt agattcctat ataaaaattc
     2281 tattttcatt aaattaaaca caaagatgaa tttatcaaga ttgttttttg tttgtcgtac
     2341 tgcttgtaat cgttcttcgg tataattgtc atgtatattg tatgttgatg gagaatcggt
     2401 aagcccttta tagcaatagt atttacgaat attatcctca tagtatgaat ggaaaaattt
     2461 atccttttgg tttttgaaaa acttttcata atttttatat tcagtaaaaa tttcatccag
     2521 gtttttaccc agtataaatg aataatcagc aatattattg attgaatgtg cagcaaaatc
     2581 aaacttaccc aatccaagaa acatgttata tgattttctg aatagtacaa tattttcttg
     2641 cacatgagca ttccaatgta aatataaata tgctaaagta aaatatacgc tgcatgcatt
     2701 tttttcaggt gttttatcta atagcttcat tgatagttgg tagaattgat ttgcccattt
     2761 gtaatcacca agccccggac ctactatacc tgctaacacc gaaaaaaggt atgaagacaa
     2821 atagcaattt cccgttttaa taataaagtt gaaaagtatg attgggccta taataaccag
     2881 attgcgactt gtataatagc cagcctgtgt tacactttca aatattcgta taactgcaat
     2941 ttcctcgggg ttttctatcc atgggagctg taataaatct tctggcttaa taaattgtaa
     3001 ttttatcttt aaagcaagca ttacctttat gacattcagc atggaaggtt tttcgggtat
     3061 gctaatccca aacattttca atccttccaa tcccagcctt accgcttctt catactgccc
     3121 accattggtt aaaaataaac atcggttaat gtatatttcg gctttatcaa ggcggctttg
     3181 tgcataatgc gataaaacat caaatagtgt agcagcatta ttttgatcac ctgctaaata
     3241 ttcactttct gcataattaa gataaatctt gaatgttagc ctgtagtaat caatccatgc
     3301 attttctgga agaagttcag cagcctgttt aaaataaatc agtgaagaat caaatgcaga
     3361 cgaattttta gccttttcac cggctattaa atttaattca gccaattgta tacgttcttc
     3421 aacagtttga atcaaatgta ctgatttatt aagatgataa acaatgtaaa atattttatc
     3481 ctgaatattt tcttttgctg ttttactcaa tgaattttta ccaatcttat aatgaagctt
     3541 actccgctcc tcttcatcca gcaatgaata tgcagcctct tgaattctat catggtaaaa
     3601 tctataaata tttttaaata atccaataaa tccaaactca atgagtgaat tcattacttt
     3661 taagattgct tctgcggttt cacctgttat tgcaatgata tcctccagtt caaatctatt
     3721 gccaatacat gcacaaatag ttagcaattc aatgttttcc gcaggcatct tgtttataga
     3781 ctgtgataac atatcaacaa cattatctgt tattggcaat tccttaacct tttcaatatt
     3841 ccatatccat cctttttcgg ggctaaatgt tattaattta tcttcaaaga gacgttttaa
     3901 aaattgtata ataaaaaaag gattgcctgc tgttttgcta taaatcaacg aagacaattc
     3961 catagtagac tcaacattat tactaagaaa atttaataga aatgtattaa catcttcttc
     4021 ttttaaaggt gctaatgcaa tgatattgat agttacacca ttatccttta tctcatgcat
     4081 gactggatta ataggatgag aggcatcaat ttcattatcc cggtaagcca tgattaaaaa
     4141 gaaattttta acggattcat caagaactat cgaacgtatt agattaagac tggctaaatc
     4201 tatccattgc acatcgtcta aaaataaaac aaccggatgc tcctgtacgg caaaaacatt
     4261 tataaacttt ttaaaaagat aattgaatct attttgcgat tcctcagggc ttaactgttc
     4321 tacaggaagc tgtggcccta tgatatgttc taaatccggg atgacatctg ttaaaatttt
     4381 accgcttttc ccaaatgcat ttaaaagctc ttctttccat aattccagct ttgataaatc
     4441 ttctgttaaa atctgtcgta tcaacatctg gaaagactga atgattgagc tatatggtat
     4501 atcacgttta aactgatcat atttacccga aataaaatat ccctttgttg cagttattgg
     4561 cttatagatt tcattaacaa gtgcagattt gccaatacca ggcgaacctt taacaataac
     4621 tatctccctg tgtccttcca taaaattttt atacgaggac aataaggatt gtatttcact
     4681 gtctcgacct acaatttgtt gaggaatatt aaaacgcaac ggaatatcaa aagaagcaat
     4741 tttaaatggt gatattgtac cttttttagt gtattcgtca ttgcattgaa tcaaatcgcc
     4801 cactatgcca aacgaatttt gatatcgttc ttcgggagtt ttagctaaca ttttatctat
     4861 tatttgcgaa acaataccag gaatatgtgg gtttacctca ctgactggca cgggcttacg
     4921 agcgatatgt gcatatatta tttcaatggg atcatcagat ttgaatggaa catttcccgt
     4981 taataactca taaaaagtaa tacccatgga atagatgtca gttctataat cgatattgcg
     5041 gtttaatcga cctgtctgtt caggtgacat gtagagcaaa gtaccttcca taatatcttt
     5101 cctgtatatt tcctgatatt cgttgggaaa caatgtccgt attccaaaat cagtaatttt
     5161 tatagtatgt aaatcttcgg ttagcagtat attggatggc ttaatatcct gatggactat
     5221 gttatattga tgaaaagtgc caacaatctg tgaaacctta atggcgatgg taaagaattc
     5281 attgagcgct actatttttt tcttcattaa aagatctttc aaggatatgc tatcaaaatc
     5341 ttctacgact atgccaaagc cttcatgtgt atcaataaat tcataaggtt ttactattcc
     5401 gctaatatct ggattcatga ttatttcgta ttcctgttta aaacgtataa aatctgacaa
     5461 tgaatactgt ttgatgttga ataccttcaa tattgcagta ctatccttat actcaatacg
     5521 gtatgttatt gttcgtggtg attcagcgat cttttgtata atttgcattc ccaatatttt
     5581 ttccattgaa atatctccct aacacctgat aactattgat atggaactat acatcgacat
     5641 tgttataaat gtagacaata tataaattaa gtccagcctt atttttttga tgatgaataa
     5701 attttgtatc atatatcata aacatttatc atggtgagat aaacatgatg tagcgataca
     5761 ttatacataa tctcccttta ttatgatttt caatcgatat cagtccatgc atgttcatta
     5821 ttatatgatt agtgtataat gattgtacaa ttttactaca ttaaagtttt tattgcaggt
     5881 actatcgccc aatatagcaa acttaaaaca tatcaagttt tgcttgacgt attgtcatta
     5941 atggtactat acaataaacc atcaactata acaaggataa tttatgagct atcctcttca
     6001 tgatatgaaa atactggatt ttacctattt gctaccagga ccttatggga gcatgatgct
     6061 ggcagattta ggagctgata ttattaaagt tgaaaatcca cataatcccg atatgttacg
     6121 tatgcttccc cccattgttg atgggatatc tgccgtttat gctcacctca acagagggaa
     6181 aaaatcattg gcattggatt taaaagcacc tgatgctaac aatattatct ataaactcgt
     6241 caaagagtat gatattgtaa tagaacagtt tagaccgggc gttcttgaaa aattagggtt
     6301 tggctatgaa gatttaaaaa atatcaatca aggcataatc tactgctcgc taacaggata
     6361 tgggcatacg ggaagttatg ccagccgtgc aggacatgat attaactata tggcactatc
     6421 aggagttgaa tca
//
LOCUS       DAOSOX010000138         6354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12348_length_6354_cov_8.490872, whole genome shotgun sequence.
ACCESSION   DAOSOX010000138 DAOSOX010000000
VERSION     DAOSOX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12348_length_6354_cov_8.490872"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..574
                     /locus_tag="PLX22_09355"
     CDS             77..574
                     /locus_tag="PLX22_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20143.1"
                     /translation="MKQQNIIPLNKVEKTERYIVVPRLIKGKVHKVVKPLKKIKGNCL
                     FTCNMPHTLIDFIYRSVKKLHLQDNKYIFSRGNVYINNKVVHNAVSTVTLEWDLGTSF
                     IIPLRLQFNSFVIIEVDNKDYSVRLMELVILIALMAQAYPSLPRSKHVSNAQRVLTLG
                     WKSIE"
     gene            complement(578..2107)
                     /locus_tag="PLX22_09360"
     CDS             complement(578..2107)
                     /locus_tag="PLX22_09360"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HOT20144.1"
                     /translation="MVDDYKDISEHLVFLNKVYELKRKRIRFMQLATVDRQLHTMADN
                     FYSLWNKTSKGFILKDAHIHESSIDTLKGLFGLHDEITIISKGNKAILTFDKIRFYQY
                     LVRHVANIHACSYLYNIFYGEENISINTLVKEQLPYLERYIPTFKKFDKAKDIASFVF
                     SQFDIENLWSIIEIYDRIKDNLIKDTALYKQFVNDFTRLYREDIALKILGYGEISTVM
                     QTIHGWQIDKTLLKMQLYEPDWVWKRMPPINSFQDVEALKKAYHEYREILIQKIGIQV
                     PEQQFRYFKNNGWYTVYAGQKKINPQMVGNVLVKRLDEVNALALFNKVLSELKRWYLF
                     NKTDSSIQVGIDGQISNWVLLSADGALNYVGSNDTLLYIDTSTPLYRINGVEQLNAEL
                     FLKNIPWFLRSIIRALFLQEVLDRYYDLRSVVVDCIANLYKEKREDLIPACIKAANYF
                     FSTMDESLQPLTLHEIVKYYQGDAFIWRLFQFARKVDKFVTERIFQKKYMYRLPKKIE
                     R"
     gene            complement(2117..3343)
                     /locus_tag="PLX22_09365"
     CDS             complement(2117..3343)
                     /locus_tag="PLX22_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT20145.1"
                     /translation="MDKEKIMDLYAQHVSPGKVEMYKKYDIALVPHKRRGVYLYDISG
                     KRFINCHCNGGVFNLGHRNKIIMDAVKKAMQRYDIGNHHLISKPKALLANKLAATLPQ
                     GLNQVIYGVSGGEAIDTAIKLARAVTGRPTVLSAIGGYHGHTGFALATGDEKFKKPFE
                     PMPPGFQQVPFNAIDALESVIDDKTAAVIFETIPATLGIVVPNKDYFKKVQALCEKHG
                     ALFIIDEVQTGFGRTGAMWGFQHFDVTPDIVVMGKGMSGGIYPITATVYKEQYAYFIK
                     ENPFIHISTFGGSEIGCFAAMAVLDLCDSNLLAHVQTMENYLKEKLAALTLHYPETPF
                     TVHGLGLMLGLAFHDEMTALFMVKLLYDNGVYVVYSGNDPRVIQFLPPLIITKKEADM
                     VMAAIDKSLEALSQSK"
     gene            complement(3582..6062)
                     /locus_tag="PLX22_09370"
     CDS             complement(3582..6062)
                     /locus_tag="PLX22_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT20146.1"
                     /translation="MSKKILSFSALIFATLLFTLVCSNGQHNTLSLTTEEVHYLKALK
                     KPIIAAPCPDYPPIDFTGSDGQFTGLTHDYLHEIGKKLGITFTVANISSWAHVLQAAK
                     EKKVDIVLSIQDVPERHSYLHFTKPYVKIPNVIVVRKSENRNFTVQDLKGLHVAAVKG
                     YAVVDDIIKKYPSIRFKFDLVTNETEGLMKLAFGEYDAMIISLSTVSYLIDTYSITNL
                     RVAGTVGYDWNLCIGVRSDNQVLYSVIAKALNSLPKTKRDEIYRKWISLAPRPVYYEI
                     QFWYIIASILLGVSIIILVIIGWNTSLRKQVAIKTQQLENELQLHKKTQEQLLLEKER
                     LFITIQSIGDIFIITDNEGNVILANKQAETVFGKAVVSGGCLYSSITFIDPNAQHTVN
                     PIDACMQSGNVTTFYHCICRYDDRSFDVSVVSSPIIIEDTVTGVVTIMRDISTMLAMQ
                     NELIKLQQFESLGILGAGIAHDFNNILMAIMGNTELLGAVLKNTGNVNDSTRITEGIK
                     KSVKSAQSLTAQLLTYARGGKPIKQSGNIVQLLNDTVDFMVHGTGVTINYSIDSYDYE
                     FDFDESQLSHVFRNLTLNAVQAMGNRGVLTIKVLEESMDTLNTIQLPAGNYVKIQFID
                     TGNGIPPEHVNKIFYPYFTTKEKGSGLGLTTSLSIVKQHGGTMTVSSSHEGTTFSVYL
                     PKVKQVQVNDTAMAHEVASPNAVVLVIDDRQDIIEVTTGMLDILGYSYDWAYSVNEAI
                     AKLVEGKNKKHLFDCVLVDVTIPGDLSGVEAFTKMKTIQPLLKGIVMSGYADNNAIAE
                     YDRYGFSWYLVKPFTLSDLKLALLKALL"
BASE COUNT         2009 a         1206 c          988 g         2151 t
ORIGIN      
        1 atttttttat gattgactaa taatagaacc atgtgccaat ttaatcaagt acattataca
       61 attactttta ccatttatga aacaacaaaa tattatccca ttaaacaagg ttgaaaaaac
      121 cgaacgttat attgttgttc cacgcctgat taaaggaaaa gtgcataaag ttgttaaacc
      181 attaaaaaaa ataaaaggca attgtctttt tacctgtaat atgccccata ctcttattga
      241 ctttatttac agaagtgtaa agaaattaca tcttcaggat aacaaatata ttttttctcg
      301 agggaatgta tacatcaata ataaggttgt tcataatgct gtatcgacag ttactttaga
      361 atgggatctt ggaacatcat ttatcattcc gctacgatta caatttaact cctttgtgat
      421 aattgaagtt gataataaag attatagtgt acgacttatg gaattagtaa tacttattgc
      481 gctgatggca caagcttacc cttcactacc aagaagcaag catgtaagca atgcacaacg
      541 ggtactgaca ctgggatgga aaagtattga ataactttta ccgttctatt ttttttggta
      601 atctatacat atatttcttc tggaaaatcc tttcagttac aaacttgtct accttacgtg
      661 caaactgaaa aagacgccag ataaaagcat cgccttgata gtattttaca atttcatgaa
      721 gtgtaagagg ctgtagtgat tcatccattg tactgaagaa ataatttgct gcttttatac
      781 atgctggtat taaatcctct cgtttttcct tatataaatt tgcaatgcag tcaaccacta
      841 ctgatcgtag atcatagtaa cgatcaagta cttcctgtaa aaataaagca cgtataattg
      901 atcgtaaaaa ccacggaata ttttttaaaa aaagttctgc attgagttgc tcaacaccat
      961 tgatgcgata taatggcgta ctggtatcga tataaagcaa tgtatcgttg ctgcccacat
     1021 aattaagtgc accatcagca gatagtaaca cccagttgga tatctgtcca tcaataccaa
     1081 cctgaataga actatcagtt ttattaaata aataccatct tttcaattca gataatactt
     1141 tgttgaaaag tgccagcgca ttgacttcat ctaaccgttt gacaagtaca ttgccaacca
     1201 tttgcggatt aatttttttc tgccctgcat atactgtata ccagccattg tttttgaaat
     1261 atctaaattg ctgttctgga acctgtatcc caattttttg tattaaaatt tctctgtatt
     1321 catgatatgc ttttttcagc gcctcaacat cctgaaaaga atttatagga ggcatacgct
     1381 tccacaccca atccggttca tataattgca tttttagcaa tgttttatca atttgccaac
     1441 cgtgtatggt ctgcattact gtggagattt caccataacc caatatttta agtgcaatat
     1501 cctcgcgata aagccgcgta aaatcgttaa caaattgctt atataaagca gtgtctttta
     1561 ttaaattatc tttgattctg tcgtatattt caataattga ccataagttt tcaatatcaa
     1621 attgtgaaaa aacaaatgat gcaatatctt ttgctttatc aaattttttg aatgtaggga
     1681 tatatcgctc aagatatggt aattgttctt taaccaatgt gtttatagaa atgttttctt
     1741 caccataaaa aatgttatat aaatagctgc aggcatgtat atttgccaca tgtcgcacta
     1801 aatattgata aaatcgtatt ttgtcaaagg taagtatagc cttattacct tttgagataa
     1861 tagttatttc atcatgcaat ccaaacaatc cttttaatgt atcaatgcta ctttcatgaa
     1921 tatgtgcatc tttcagaata aatccttttg atgttttatt ccacaatgaa taaaagttat
     1981 ctgccatggt atgcaactgc ctgtccactg ttgccagttg cataaaccgt atgcgttttc
     2041 gctttagttc gtaaacttta tttaaaaaga ctaaatgttc tgaaatatcc ttatagtcat
     2101 caaccataac gacacctcac ttagattgcg ataaagcttc aagtgattta tcaatggcag
     2161 ccattaccat atcagcttct ttttttgtaa tgatcaaagg tggtaaaaat tgtatcaccc
     2221 gcggatcatt tcctgaatac acaacatata caccattatc atataaaagc tttaccataa
     2281 acagtgcggt catctcatca tgaaatgcaa gtcctaacat caatcccaat ccatgtactg
     2341 taaaaggagt ttcaggatag tgtaaagtta aggctgcaag cttttctttt aaatagtttt
     2401 ccatggtctg cacatgcgct aaaaggttac tatcgcacaa atctaaaact gccattgcag
     2461 caaaacaacc tatttcactg ccgccaaacg ttgagatatg aataaacgga ttttctttaa
     2521 tgaaatatgc atactgctct ttatatactg tagcagttat tggataaata ccaccactca
     2581 ttcctttacc cataacaaca atatctggcg taacatcaaa atgctggaac ccccacatag
     2641 cgcctgtacg cccaaagcct gtttgcacct catcaatgat aaatagtgca ccgtgctttt
     2701 cacacagcgc ctgtaccttt ttaaaataat ccttattggg aacaacaata ccaagcgttg
     2761 ctggaattgt ttcaaatata acggctgctg ttttgtcatc aatcactgat tccaacgcat
     2821 caatagcatt gaatggaact tgttgaaatc ccggaggcat tggttcaaat ggctttttaa
     2881 atttttcatc acccgttgcg agagcaaatc cggtgtgtcc atgataacca ccaattgcag
     2941 ataatacagt agggcggcct gtcacagctc gtgctaattt tattgcagta tcaattgcct
     3001 cgccacctga aacaccataa ataacctgat tcaatccctg tggtaacgta gcagctaatt
     3061 tgttagccag taaagctttt ggcttgctta ttaaatgatg gttgccaatg tcgtaacgct
     3121 gcatcgcttt ttttacggca tccattatta ttttatttct gtgtcctaaa ttaaaaacac
     3181 caccattgca atggcagtta ataaaccgtt tcccggagat atcatataaa taaacgcctc
     3241 gcctcttatg gggcaccagc gcaatatcat attttttata catctccact ttgccgggcg
     3301 agacatgctg tgcatacaaa tccataattt tttctttatc cataaaaccc ccatgtttta
     3361 tattacaata cttttataaa tatgtcaaaa tctttttttt catgtgcgat agtcactgta
     3421 aattgaatca tcatataata taaaaatgat aatcattata tcatactcgt tttttatatt
     3481 gtgaagaggt agtctattat aaaagagtta ccagtggctg agatacaaat aaccatagtt
     3541 taatagtttc aatagtagtg actgctgcca ttgtaacctg gttacaaaag cgcttttagc
     3601 agggcaagtt ttaaatcact taatgtgaat ggttttacca gataccatga aaatccatac
     3661 cggtcatatt cagctattgc attattatca gcataaccgc tcatgacgat accttttaaa
     3721 agtggctgta ttgttttcat tttggtaaat gcttcaactc cactcaaatc ccctggtatc
     3781 gttacatcta ccagtacaca atcaaacaga tgttttttgt ttttcccttc aaccagttta
     3841 gctattgctt cattaacgct ataagcccaa tcatagctat atcctaatat atccagcatc
     3901 cctgttgtta cttctattat atcctgacgg tcatctatta ccaataccac ggcattgggt
     3961 gatgcaactt catgagccat tgcggtatca ttaacctgta cttgttttac ttttggtaaa
     4021 taaacgctaa atgttgtccc ttcatgcgaa gatgatacag tcatagttcc accatgctgt
     4081 ttaactatgg aaagcgatgt agttaaacca agccctgaac ccttttcttt tgttgtaaaa
     4141 tacggataaa aaattttatt tacatgttcc ggagggatgc catttcctgt gtcaataaac
     4201 tgtattttta cataattacc agcaggcagt tgtatggtat ttaaagtatc catactttcc
     4261 tctaatacct tgatggtcaa cacccccctg ttgcccatag cttgcaccgc atttaatgtt
     4321 aaatttctga atacatgcga tagttgactt tcatcaaaat caaattcata atcataacta
     4381 tctatactat aattaattgt tacgcctgtt ccgtgtacca taaagtctac tgtgtcgtta
     4441 agcaattgaa caatattacc ggactgtttt attggttttc cacctctggc ataggtcaat
     4501 aactgtgccg taagagactg tgccgatttc accgactttt taatcccttc agttatacgg
     4561 gtgctatcat ttacattgcc tgtatttttt aacacagctc caagcaactc tgtgttaccc
     4621 ataattgcca tgagtatatt attaaaatca tgagctatgc cagcacccag tattcccaac
     4681 gattcaaatt gttgcagttt tataagttca ttctgcattg caagcatggt gcttatatca
     4741 cgcattatag ttacaactcc cgtgacagta tcttcaatga tgataggtga agaaactaca
     4801 gaaacatcaa atgatctatc atcatatcta catatacaat ggtaaaatgt agttacgtta
     4861 cccgattgca tacatgcatc tatagggtta acggtgtgtt gagcatttgg atcaatgaaa
     4921 gtaatggaag aatataagca ccctcctgat acaactgctt ttccaaatac tgtttctgcc
     4981 tgtttatttg ccagtatcac atttccttca ttatcagtga tgataaatat atcaccaata
     5041 gattgtatgg taataaacaa acgttctttt tccagcaaca actgttcctg agttttttta
     5101 tgcaattgta attcattttc aagttgctgt gtttttatcg caacctgttt tcttagcgaa
     5161 gtattccacc caattataac cagtataata attgacactc ctaaaagtat ggatgctatg
     5221 atataccaga attgaatttc ataataaaca ggtcttggtg ctagtgaaat ccactttctg
     5281 taaatctcat ctcgttttgt ttttggtaaa gaattgagcg ctttagcaat gactgaatat
     5341 aatacttgat tatcactgcg aacaccaatg cacaggttcc aatcatagcc aacggttccc
     5401 gcaacacgta aatttgttat actgtaggtg tcaattaaat atgaaacagt tgaaagagag
     5461 attatcatag catcatattc accaaaagcc aacttcatca acccttcagt ttcatttgtt
     5521 acaagatcaa atttaaatct aatagaagga tattttttta taatatcatc taccaccgca
     5581 taccccttta ccgcagcaac atgcaatccc tttaaatcct gaactgtgaa atttctattt
     5641 tcagattttc gtacaacaat aacattggga atttttacat agggttttgt aaaatgcaaa
     5701 tatgaatgcc gttccggcac atcctgaatg gataatacga tgtcaacttt tttttcttta
     5761 gcagcctgta atacatgtgc ccatgatgat atattggcaa ctgtaaaggt tattcctaat
     5821 tttttaccaa tttcatgtaa atagtcgtga gtcaatccag taaactggcc atcactacca
     5881 gtaaaatcaa tagggggata atcagggcat ggcgcagcta taataggttt ttttagtgct
     5941 tttaaataat ggacctcttc cgttgttaag gataaggtgt tatgctgacc gttgctgcat
     6001 acaagagtaa ataaaagtgt tgcaaatata agagctgaaa atgataatat ttttttgctc
     6061 atcacaactc caggacacca ttacttttca ttagtttgtt gtaatagtaa tggcaattgt
     6121 atgaaaaaac aagtataaaa ttaaaatggt ttttatattg ttagtgtcat tgatagtaca
     6181 ggaagattgt ctgaatttct gctacagtat tttacatgct gtatgttttt ttattacgtg
     6241 tatatgtata catattactc tataatttac ttaagcgata gcaactatca ataaaagata
     6301 tctttaaatg aatagatgga acgactggtt tgaacagggg aaaagagatt ttga
//
LOCUS       DAOSOX010000139         6319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12436_length_6319_cov_8.712803, whole genome shotgun sequence.
ACCESSION   DAOSOX010000139 DAOSOX010000000
VERSION     DAOSOX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12436_length_6319_cov_8.712803"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLX22_09375"
     CDS             <1..477
                     /locus_tag="PLX22_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20147.1"
                     /translation="TTVNTKYGPIKTNHILFIAAGAFNVSKPSDLIPELQGRFPIRVE
                     LSSLSEEDFRKILTVPKNSLTLQYKELLKTEGVAIEFTRDGIVEIARIAQVLNDENEN
                     IGARRLHTIMEKLLEDILFDAPDLDTVHKNFTINADFVKNKLEPTIKNKDLSKYIL"
     gene            complement(483..1391)
                     /locus_tag="PLX22_09380"
     CDS             complement(483..1391)
                     /locus_tag="PLX22_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOT20148.1"
                     /translation="MNESKSIRYICKEHHLRIYATISKDLLEQLISIHHTTLHATIAL
                     GRTLNAAILLASSSLKPNSNDTLSIKIQCSGPLQEIMVQVDGRGNVRGLVSNPDVDAM
                     MQTQTLDIAKALGAGIITVTRDLGLKEPYTGVSPLLYGDIANDIAHYLTQSEQIPSAI
                     ILTVNYNNDGTLARSAGILIQTYPDTPEFVIQHIEQYLQSGFSLDRELQTTTDCKAII
                     EKIVKSNIEPLGCTPLQLNCRCNKEMLTQILVSIDNNELTAMIEKDHGAQITCAFCKK
                     QYDFTEDDLKKILLTKEKKSPNTSYQ"
     gene            complement(1384..>1851)
                     /locus_tag="PLX22_09385"
     CDS             complement(1384..>1851)
                     /locus_tag="PLX22_09385"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20149.1"
                     /translation="VRIYPTIVIKDTVLEKLFIEGIYTPMTLEEAVTLTAGMYSIFTM
                     NNIPVIRMGLHPFANDTSSFIVAGPYHPSFGYLVKSRYCRNQLQNMLQQATDNNSTSK
                     RYIIELPALHSAEYLGAHRENIAFLQNMFYNSYIQCTITNTLEPILYSDSYYE"
     assembly_gap    1852..1951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2062..3915)
                     /locus_tag="PLX22_09390"
     CDS             complement(2062..3915)
                     /locus_tag="PLX22_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOT20150.1"
                     /translation="MNALWIVTAAATFYMLAYRFYGAFLAAKVLALDDSRVTPAYRLN
                     DNTNYYPTNKWVLFGHHFAAIAGAGPLIGPVLAAQFGYLPGFLWILIGSVFAGGVHDM
                     VILVASIRKDGKSLAEIAKEEIGPVTGMAALFAVLFILIVAIAGLGLVVVNSLFNNPW
                     GTFIIAMTIPIAIVMGIYMKSIRPEKVLETSIIGIALLLITVIYGRNILGTGAEPLFT
                     LNQHNLAVTLAIYGFIASVLPVWLLLAPRDYLSSFMKIGVILLLAIGVLVVHPDLHLP
                     ALTQFIHGDGPIIPGKLFPFVFITIACGAISGFHALISSGTTPKMIEKERYALPIGYG
                     AMLVEGFVSMMALIAAAVLIPGDYLAINTHLSFETLAQMGFPVDKINELSQLVGINIA
                     GRPGGAVSLAVGMAYIFSSIPFLSHLMAYLYQFAIMFEALFILTTIDAGTRVARYVVQ
                     ELGGYIYKPFGDLRSLYGNIIASLIVVFAWGYFIYTGSVQTIWRMFGTANQLLAMLAL
                     CIGTTIIIRMNKIKYIWVTLIPMLFMLTTTFTAGIILIMEYIPLALSTQPDAYTYMIN
                     IVLLGLMILLALFILLDSMYKWYGFITGKRPLQNNACEPSQYSKEFPNMSD"
     assembly_gap    3925..4024
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4025..5085)
                     /locus_tag="PLX22_09395"
     CDS             complement(<4025..5085)
                     /locus_tag="PLX22_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT20151.1"
                     /translation="MRSSTFYIIFASIIAFYLIANYYILTRIQKLVPQQFKLFTVAGI
                     AILALSFIMGRILERYTVCATSDFLVWIGALWIALFLYLLLGFLLTDSIAAITGIFFN
                     ISNVKRYAYTVIIILSFAVTVAGYINELKPVVKEINITIHKPSSLKQLRIAYASDIHL
                     GSIIANSRLENLVTLINNSNPDIIIFGGDIVDEDIKPVIENNLGELLLQLKANHGIIA
                     VPGNHEYIGGIDKAMNYIKSHNIKILRDELFTINDIQIIGRDDRSKFAFTGEHRAPLE
                     HLIKKVNTNHPLIIIDHQPFAIAKTASYPVDLLLSGHSHHGQLFPFNYITSKLYQISW
                     GYDRINNTHVYVSCGYGTWG"
     gene            5415..6128
                     /locus_tag="PLX22_09400"
     CDS             5415..6128
                     /locus_tag="PLX22_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20152.1"
                     /translation="MEHIKKVRWLLIIMVIIGAMYLTYAYPDQVFRSNEFIQSDISPD
                     RLQFMPVTEDFRNYFVLQCINNTTSILIGDFTGTEKNISLAKDTNGDGKLDEVFEYFP
                     EIKKLTTPLKPTTAFFSGIETLKKDIILGDIYAVNYAYKMNSLPLLKAKLKSGRDVFK
                     FKHGYSVKIYDPDAPTTIMGEYFFGKKEGKYDLIFSTYYYKLYRTKISPPLSYSVYCK
                     DTTDKYIAEIVEELLKIVQ"
BASE COUNT         1961 a         1081 c         1142 g         1935 t
ORIGIN      
        1 acaacggtaa ataccaagta tggtcctata aaaacaaatc atatactatt tattgctgct
       61 ggtgcattca atgtgtcaaa gccttctgat cttatacctg aacttcaggg aaggttcccc
      121 atcagggtgg aattatcttc tttatccgaa gaagatttta gaaaaatcct cacagtacct
      181 aaaaactcat taacgcttca atataaggaa ttattaaaaa cagaaggagt ggctattgag
      241 tttaccagag atggtatagt agaaattgct cgcatagcac aggtattaaa tgatgaaaat
      301 gaaaatattg gagcacgccg attgcataca ataatggaaa aattgcttga ggatattttg
      361 tttgatgcgc ctgatcttga tacggtacat aaaaacttta ctatcaatgc tgattttgta
      421 aagaataaat tagaacctac tataaagaat aaagatttat caaaatatat actgtaactt
      481 ttttattgat agctggtatt gggtgatttt ttttcttttg ttaaaaggat tttttttaaa
      541 tcatcttctg taaagtcgta ttgtttttta caaaatgcac atgttatttg tgcaccatgg
      601 tctttttcta tcatcgctgt aagttcatta ttgtcaatag atacaagtat ctgggtaagc
      661 atttccttat tacacctgca gttgagctgc aatggtgtac atcctaatgg ttctatgtta
      721 cttttaacga ttttctcaat tattgcctta caatcagtag ttgtttgcaa ttccctgtct
      781 aatgaaaaac cactttgcag atattgttct atgtgctgaa taacaaattc gggtgtatcg
      841 ggatatgttt ggatcagtat cccagcagag cgtgctaacg ttccatcatt gttataatta
      901 acagtaagaa tgatggccga aggtatttgt tcagattggg ttaggtagtg ggcaatatcg
      961 tttgcaatat caccataaag aagaggtgat acacccgtat atggttcttt taatcctaaa
     1021 tcacgagtta cagtaattat acctgcaccc aatgcttttg ctatatcaag tgtttgtgtt
     1081 tgcatcatgg catcaacatc tggattgctt accaatccac gcacattacc cctcccatct
     1141 acctgtacca taatctcttg caatggcccg ctgcactgaa ttttaatgct taatgtgtca
     1201 tttgagtttg gctttaatga tgaagaagcc agtaagatcg cagcatttaa agttctcccc
     1261 agtgcgatgg ttgcatgaag ggttgtatga tgtatggaga ttaattgttc cagcaaatct
     1321 tttgagattg ttgcatatat tctgagatgg tgctctttac aaatgtatcg tatgcttttg
     1381 ctttcattca tagtaactat cgctatatag tattggttcc agggtatttg ttatggtgca
     1441 ttgtatataa ctattataaa acatgttttg taaaaatgca atgttttctc tatgagctcc
     1501 cagatattct gctgaatgca aagctggcaa ttcaatgata tagcgttttg aggtactatt
     1561 gttatccgta gcctgctgca acatgttttg tagctgattg cggcagtatc gtgatttaac
     1621 aagatagcca aacgatgggt gatatggtcc tgcaactatg aagctgctgg tgtcatttgc
     1681 aaagggatgt aatcccatcc ttatgaccgg aatgttattc atggtgaaga ttgaatacat
     1741 cccggcagta agtgttactg cttcttccag tgtcattggc gtatagattc cctcaatgaa
     1801 aagcttttct aacactgtgt ctttaataac gatggtaggg taaatcctta cnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtattactg gtatacacct ggttgcagaa
     1981 cacacagcgg tgcctgcaac caaggtgagg tatgaagaat ggtattgtta catttgtttt
     2041 tgacattcaa ctccttgatg tttaatcgct catattggga aattcttttg agtattgtga
     2101 aggttcacat gcattgttct gcagtggtcg tttgcctgtg ataaaaccat accacttata
     2161 cattgaatca aggagtataa ataacgctaa cagtatcata agtcccagca acacaatgtt
     2221 tatcatgtag gtgtatgcat ccggttgtgt tgacaatgca agcggtatgt actccataat
     2281 aagaattatc cctgcagtaa aggtggtagt taacataaaa agcatgggga tgagcgtaac
     2341 ccagatatat tttattttgt tcatcctgat gatgatggtt gttccaatac acaatgccag
     2401 cattgccagc aattgattgg ctgttccaaa catgcgccat attgtttgca ccgaaccggt
     2461 ataaataaaa tacccccacg caaaaacaac aataaggctt gcgataatat tgccgtataa
     2521 tgatctgagg tcgccaaaag gtttgtatat atagccacct aattcctgta ctacataccg
     2581 tgctacacgt gtaccagcat caattgttgt cagtatgaac agtgcctcaa acatgatggc
     2641 aaattgatac aggtatgcca tgagatgact taagaaagga atggacgaaa agatatatgc
     2701 catcccaacc gcaagtgata ccgcaccacc aggcctgcca gctatgttta ttccaaccag
     2761 ttgtgatagc tcattaattt tatctaccgg aaaacccatc tgtgccaggg tttcaaagga
     2821 aaggtgagta ttaattgcaa gatagtcacc ggggataagt acagccgcag caatgagtgc
     2881 catcatggaa acaaatccct caacaagcat tgcaccatat cctattggca gtgcataccg
     2941 ttctttctcg atcatctttg gtgtggtgcc tgatgagatg agcgcatgaa atcctgaaat
     3001 agcaccacag gcaatggtga tgaacacaaa cgggaaaagc ttaccgggta taattggccc
     3061 atcgccatgt ataaattgtg ttaatgccgg tagatgtaaa tcagggtgca ctactaaaac
     3121 gccaattgct aaaagcagga tgacgccaat tttcataaat gatgagagat aatcacgggg
     3181 ggcaagcaac agccataccg gtaaaacgct tgcgataaaa ccatatatag caagtgtaac
     3241 agcaaggtta tgctgattta atgtaaaaag aggttcagca cctgttccta aaatgttcct
     3301 gccatagata acagtgataa gtagtaacgc aattcctatg atagaagttt ccaatacttt
     3361 ttcggggcga attgacttca tataaatgcc catcacaatt gctattggta tggtcattgc
     3421 aatgataaaa gttccccatg gattattgaa taatgaattt acaacgacaa gccccaatcc
     3481 ggcgatagcc actattaaaa taaacaatac tgcaaagagc gctgccattc ctgttacagg
     3541 acctatttct tctttggcta tctcggcaag cgactttcca tccttacgaa tagaagcaac
     3601 cagtataacc atatcatgaa caccgccggc aaagaccgaa cctattaaaa tccataaaaa
     3661 gcctggaaga tacccaaact gagcggctaa cacaggtcca attaaaggac ctgcaccggc
     3721 aatggctgca aagtgatgcc caaataatac ccatttattg gtaggatagt aattggtgtt
     3781 atcattcagc cggtaagcag gtgtgacgcg ggaatcatct aatgcaagta ccttcgcagc
     3841 taaaaatgca ccatagaatc tgtatgcaag catatagaag gtggcggcag cagttactat
     3901 ccacagtgca ttaatctttt cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnccccat gttccataac cgcacgaaac atacacatgg gtgttgttta tacgatcata
     4081 tccccagctt atctggtata gttttgatgt tatgtaatta aaaggaaaca actgtccgtg
     4141 atgtgaatgc cccgaaagca aaagatcaac agggtatgat gctgtttttg caatggcaaa
     4201 tggctgatga tcaataataa tcagtggatg gtttgtgttt acttttttta taagatgctc
     4261 cagaggagca cggtgctctc cagtaaatgc aaatttgctt ctgtcatcac ggccaataat
     4321 ctgtatatcg tttattgtaa atagttcgtc ccttaagatt tttatgttat ggcttttaat
     4381 gtagttcatg gctttatcta tcccgcctat atattcatgg ttgccgggaa cggctatgat
     4441 accatggtta gcctttaact gtagcagcaa ttcacctaaa ttattttcaa taacaggttt
     4501 tatatcttca tcaacaatgt cgccgccaaa tataatgatg tcgggattag aattgtttat
     4561 taatgttact aaattttcta aacgcgagtt ggcaataatg gatcccaggt gtatatcgct
     4621 tgcgtaggca atacgtaatt gttttaatga agaaggctta tgtatagtta tgttaatttc
     4681 tttgacaaca ggtttcaact cattaatata accggcaact gttacagcaa aacttaagat
     4741 aattattact gtataagcat accgtttaac attcgatata ttgaaaaaaa tccctgttat
     4801 ggctgctatg ctatcagtta gcaaaaaacc aagtaacagg tacaaaaata aagctatcca
     4861 caatgctcca atccagacaa ggaaatcact ggtagcacat acggtatatc gttctaaaat
     4921 tcttcccatt atgaacgata gtgccagtat ggcaatacct gcaacggtaa atagtttaaa
     4981 ttgctgaggg actaatttct gaatgcgggt aagaatataa taattagcaa taagataaaa
     5041 agctataata cttgcaaaga tgatataaaa ggttgaactt cgcatactta tattcctttt
     5101 tcattagtat atcgttatta atgttttaaa ccaaataatt gttacaaaaa tatacaatgt
     5161 attcttatgt tacaggtgat cgttgactat aaaaaaagta tgtctactta taaacaattt
     5221 tttattaaat taaaaaaggt aatggaatat gtatggcaaa caattttatt ttttaaaaat
     5281 tttaaacaat aaaaattatg aaatttaaaa cattattttg tatagaaaaa atttttatta
     5341 ttgactttat tttttatttt atttacaata atattaatga tataccatat tcatagttaa
     5401 tcggggttat tataatggaa catataaaaa aagttcgctg gttattaatt ataatggtaa
     5461 ttattggtgc tatgtacctt acgtatgcat atccagatca ggtttttaga agcaatgaat
     5521 ttatacagag cgatatatct cctgatagat tacagtttat gcctgtcaca gaagatttta
     5581 ggaattattt tgttcttcaa tgtatcaata atactacttc aattttaata ggagatttta
     5641 caggcactga aaagaatatt agcctggcaa aagatactaa tggcgatgga aaacttgatg
     5701 aagtatttga atattttcct gaaataaaga aattaacgac cccgcttaag cccacaactg
     5761 catttttttc aggtattgaa acattaaaaa aagatataat tctgggtgat atttacgcag
     5821 taaactatgc ctataaaatg aactcgctgc cacttttaaa agctaaattg aaaagcggaa
     5881 gggatgtttt caagtttaag catggatatt cggtaaaaat ttatgatccg gatgctccaa
     5941 caaccataat gggcgaatat ttctttggta aaaaagaagg gaaatacgat cttatcttta
     6001 gtacgtatta ctataaactt taccggacta aaatatctcc accactcagc tattcagttt
     6061 actgtaagga tacaaccgat aaatatatcg ctgaaattgt agaagaactt ttaaaaatag
     6121 tacaataata gtttatgctc cgctattttt tgccttcttg tcgcttgtgc agcacctcaa
     6181 ccatttttac aaacagagca taaaagtcct gtaactcatc ctgcttgcgc agtgaacgtg
     6241 ggttagggat aatgccgtta attatatcat taaaataatt tgacataaca tataatggcc
     6301 ctgctatctt atgcgtttt
//
LOCUS       DAOSOX010000140         6200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12740_length_6200_cov_8.193979, whole genome shotgun sequence.
ACCESSION   DAOSOX010000140 DAOSOX010000000
VERSION     DAOSOX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12740_length_6200_cov_8.193979"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..312)
                     /locus_tag="PLX22_09405"
     CDS             complement(151..312)
                     /locus_tag="PLX22_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20153.1"
                     /translation="MGKIGGQSIVKVIVQKRKKGSEHKGPGNLLLMAYMADSFAGFQV
                     KDLIGEVIE"
     gene            713..1312
                     /locus_tag="PLX22_09410"
     CDS             713..1312
                     /locus_tag="PLX22_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20154.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKNYLQEDYFKACFVQVLNKAK
                     AKYHFKLIAFCIMDNHIHLVIHTLDDGAPISRIVQYIKARFAELYNKITGRTGPFWNE
                     RYKDSIVQFAKDGFHYLLWLLWYLAYNPIRRRLCSDPTKYHYSSIQAYLTEEANIGVP
                     IDHHDYFVKLGETFAKRVARFMQYEEYYRKKYSMILEWV"
     gene            complement(1409..2452)
                     /locus_tag="PLX22_09415"
     CDS             complement(1409..2452)
                     /locus_tag="PLX22_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT20155.1"
                     /translation="MSVIDSIPFEILEAVKKENLSIEVTTRCTSNCTYCFVLSDCIEY
                     HDLDYETVFSIIMEGYEIGYRHLHITGGEPLLWGYLFDILNDAISIGYEAIFLNTNGF
                     LLDTKILQQLKSIKGLSLSISLLGFEAIHNAFRGNNSFIKTTQAIEMALYNNIPVYLF
                     CVTGKSLLPHVPHFIQWAYQKFPGIKEITLIQLIRVSEDAGNVCNELLSPDDFLFLVR
                     MISLLKIYGFPVSLLENPLAAVVAQLFQLPWFPNPLHLIRPGKLTIKADKSITIAHSS
                     SQSVGIYSRGELHRILASEVYLRGVKEDHNTCSSCNHIAACRKAGMQRPSEFFRDMNE
                     NIPYCKRVLDSIC"
     gene            complement(2439..3470)
                     /locus_tag="PLX22_09420"
     CDS             complement(2439..3470)
                     /locus_tag="PLX22_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20156.1"
                     /translation="MEKYTMFSNTDFHFGHTSINEDIAYFYTKMNDAIINLVRSLPVS
                     VQTSAMIFLMHNAGIQPGQNLDFFSNYYSPIWSIIPHIMIRHTCTRIKQEFMYLAATA
                     HAMVMLLHSLDNHINDGQVVPDHLTLLIRSQGWKLFWDTISTFCSLIHTDKEIVNSCL
                     DRYYACITTDKSIDNLEEYLRIFRDQMATALIVPLLTVQFVQDMSLDEDIRALLECFG
                     LAWRIYDDIQDMKDDLNNGRNSAVTLSLPREVRCRWNEFLKIGTIIPEGENISERKND
                     SINILEEMIQTSGVIPILTKRIIKELKAAAKHAEDIGLFGLSDEIVKLRIHFVQGMKC
                     DTGEDNVSD"
     gene            complement(3592..4677)
                     /locus_tag="PLX22_09425"
     CDS             complement(3592..4677)
                     /locus_tag="PLX22_09425"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20157.1"
                     /translation="MERLSEEIKVYKDITPENYVFSLTNDDARMKIQEIAQYLYRIKV
                     LFAHDSVSESQAYRILARLVDEQIEITGKGKGVKVKVSNELRSSCIQSPYDPDATYRE
                     KGKQASQGYSVSIVETCDPDNKLQVITHVITEKNNVDDSTILEDNFEAIVGEDTEELI
                     ADGAYANKSVQDRLSESKKTLITTAIRGRKPDSNKVRSTDFEIENNRIIRCPYGEIPI
                     HQEYNNGVIIAHYAHESCSSCPLKCMIRKNKRKPHVLVITQERLQMDRQREKYNDSEY
                     LKKCALRPAIEGTMFQFKLHLRNGKSRYRGKSKVKCSSILRTIAINFKRVYAYRLKEA
                     LSYFILRNSMIIKSFLARKNILCCFST"
     gene            complement(5002..5748)
                     /locus_tag="PLX22_09430"
     CDS             complement(5002..5748)
                     /locus_tag="PLX22_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20158.1"
                     /translation="MDIDIKTRDYGMSFLLSYAKLDADIICVLHPLSYLIKKSNFNLL
                     KNFTKNYKLVNGVIIDSGTFKETSKSISFPIIIALYKKEKTGMDYNYVQNYKFKTIDN
                     KNFSLNDFDFISNYINKYPLKNIKPNENDILFWTMRDINALKRNRTFIHEFGYNAIIV
                     DKNKLDYYIYVDVFKQFSHLIPYYFGNLDVFINNEMFNKYKDYFIYECIMRNNFLKKY
                     VQYDDNISLEKAKEIIINYFKFLTGEHYVD"
     gene            complement(5781..6185)
                     /locus_tag="PLX22_09435"
     CDS             complement(5781..6185)
                     /locus_tag="PLX22_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20159.1"
                     /translation="MTITDIQIQENKKGIDIKNQYHLKYYDKINLGAFYTPTKYVDIV
                     WEMIKPFINNKSIVLDSSCGYGNFFDYKINCPKKANDIDYIATNQTKDNFPEIDVYNK
                     NALLNVNRKMFNISDNNELIIIGNPLIMILLQ"
BASE COUNT         1945 a         1180 c          874 g         2201 t
ORIGIN      
        1 tccctgactt tacgtttgta cattttagtc atttattttc ttttcatgaa actaatttct
       61 atttctattt aattaaatac tcatgctact tatattgaag tatccctata ttatacacaa
      121 aatttcaagt aaaatatata cttaataatt tcattcaata acttccccta ttaaatcttt
      181 cacttgaaaa cctgcaaaag agtctgccat ataagccatt aaaagtaaat tgcctggtcc
      241 tttatgctct gacccctttt tacgcttttg cacgattact tttactatgc tctgaccccc
      301 tattttcccc atatttttac caatcctctc tacaattcac ctgctccatt cagtattctt
      361 ctccatataa agttttaagg atctgacccc attctattta ctttaccctt tactatcttt
      421 actatgctct gaccccatgt tggatccctt gttctgtcga tgttgcgtat ttatgtcata
      481 cttagcatta tttgtaatca ttgcttgata tatcttaaaa ttaataggct ctgacccttt
      541 gtttctgtaa ttcatctctt atctcttatt atgctctgac cctattcgat tcataaaaaa
      601 atcttcaaaa tcttaaaaaa tttttcacta tacccgttca attttcaaat cacaagcgtt
      661 atatacagta aaggctttat tttcttacct actacataca aggaggaata ccatggcacg
      721 gaaattacgt gtacaactcc caggtttaac ctatcacatc acttcccgct gcattgaatg
      781 gaaaaactac ctgcaggaag actatttcaa ggcatgcttt gtacaggttt taaacaaagc
      841 taaagctaag tatcacttta agctcattgc cttctgtatt atggataacc atattcatct
      901 tgtcattcat acacttgatg atggtgctcc tatatcccgt attgtgcagt atataaaagc
      961 caggtttgct gaactgtata ataaaatcac cggcagaacc gggccattct ggaatgaacg
     1021 gtacaaggac agtatcgtac agtttgctaa agatggcttt cattatttgt tgtggttatt
     1081 gtggtatctg gcatataatc ccatacggag gaggttatgc tctgacccta caaaatacca
     1141 ctacagcagc atacaggcat atttaactga agaagcaaat ataggtgtac ccatagacca
     1201 tcatgactac tttgtgaagc tcggggaaac gtttgccaaa agggtggcaa gatttatgca
     1261 gtatgaggaa tattacagga agaagtattc tatgatactt gagtgggtgt aaaaggagca
     1321 atcttggact cagaggctct gacccttctt ttgtcacatt gattgtgata tttatgatac
     1381 tgaataataa atagtaatcc agagcttgtt aacatatact atcaagaaca cgtttacaat
     1441 agggaatatt ttcattcata tccctgaaaa actctgatgg gcgctgcatt cctgctttac
     1501 gacatgctgc tatgtgattg caggaagaac aggtgttatg atcctcttta actccacgta
     1561 aataaacttc agaagcaagg atgcgatgga gttcaccacg gctgtatatg ccaaccgatt
     1621 gacttgatga atgggcaatt gtaatacttt tatcagcctt tatggtaagt tttcccggac
     1681 gtataaggtg aaggggattt gggaaccatg gtagttggaa caattgagct actactgcag
     1741 caagaggatt ttcaagtaat gaaaccggga acccatatat tttcagcaaa gaaatcatac
     1801 gcaccaaaaa taaaaaatca tcaggcgata acaactcatt acatacatta cctgcatctt
     1861 cagaaacacg aataagctgt atgagagtaa tttcctttat acctggaaat ttctggtatg
     1921 cccattgtat aaaatgtggc acatgtggca aaaggctttt cccggtcacg cagaaaaggt
     1981 atactggaat attgttgtat agtgccattt caatcgcttg tgttgttttt ataaatgaat
     2041 tattaccacg gaaagcatta tgtattgctt caaatcccag taagctgatt gaaaggctca
     2101 gtccctttat agatttaagc tgctgtaata ttttagtatc taataaaaat ccatttgtat
     2161 ttaaaaaaat tgcctcataa cctattgaaa tagcatcatt caaaatatca aaaagatatc
     2221 cccacaatag cggttcacca cctgttatgt gaagatgtcg atagccaatc tcataacctt
     2281 ccattattat tgagaatact gtttcataat caagatcatg atattcaata caatcagata
     2341 gtacaaagca ataggtacag ttgctggtac atcgtgttgt aacttctatt gaaaggtttt
     2401 ccttctttac agcttcaaga atttcaaatg gtatagaatc aatcactgac attgtcttct
     2461 ccagtatcac atttcatccc ctgaacaaaa tgaatgcgta atttcactat ttcatctgaa
     2521 aggccaaaaa ggccaatatc ttctgcatgt tttgcagcag cttttaattc cttaataatc
     2581 ctttttgtaa gaatagggat gacacctgaa gtctgtatca tttcttcaag aatatttatg
     2641 ctatcatttt tacgctctga tatattttcc ccttcaggta tgatggttcc tatttttaaa
     2701 aattcattcc agcgacaacg aacttccctg ggcaggctaa gagttactgc agaattccgt
     2761 ccattattca gatcatcctt catgtcctgg atatcatcat atatccgcca ggctaaacca
     2821 aaacattcaa gtaaggctct gatatcttcg tctaaagaca tatcctgtac aaattgtaca
     2881 gtcagcaggg gaacaatgag agctgtagcc atttgatccc tgaaaatacg tagatattct
     2941 tccagattat caatgctttt atccgtggtt atacacgcat aatatctatc aagacagcta
     3001 ttcactattt ccttgtctgt atgaataagt gaacaaaaag ttgaaatggt atcccagaat
     3061 agcttccatc cctgacttcg aataaggaga gtcaaatgat ctggtacaac ctgaccatcg
     3121 ttaatatgat tatctaaaga atgaagaagc atgaccattg catgagctgt tgcagcgaga
     3181 tacataaact cctgtttaat ccgtgtacat gtatgcctta tcatgatatg aggaatgatt
     3241 gaccatatag gcgaatagta attgctgaaa aaatccagat tttgtccagg ctgaatgcca
     3301 gcattatgca ttaaaaatat catcgcagag gtttgaaccg atactggaag agaacgaaca
     3361 aggttgatta tagcatcgtt catttttgta taaaaatatg ctatatcttc atttatcgat
     3421 gtatgtccaa aatgaaaatc agtatttgaa aacattgtat atttttccat tacaattact
     3481 aactgctttg ttgcagaaac atgaacccta agggtattta gtattatcca aatacatatt
     3541 tttgacaagt taattattaa tatatgattc ccctgcacaa acacactgtt tttaagtaga
     3601 aaaacagcaa agaatatttt ttcgtgctaa aaatgacttt attatcatac tatttcttaa
     3661 aataaaatag gataaggctt ccttaagcct gtaagcgtac actcttttga aatttattgc
     3721 aattgttctc agtatcgaac tgcattttac cttacttttc cctctatacc gacttttccc
     3781 atttctcaaa tgcagcttga actgaaacat tgtaccttca attgccggtc gaagtgcgca
     3841 ttttttcaaa tactcactat cattgtattt ctctcgctgc ctatccatct gtaatctctc
     3901 ttgtgtaatc acaagcacat gcggcttacg tttgtttttc cgtatcatac atttcagtgg
     3961 acaggaggaa caactctcat gagcatagtg tgctatgata acaccattat tatattcctg
     4021 atgtatcgga atttccccat agggacagcg tattatgcga ttgttttcaa tctcaaagtc
     4081 agtacttcgt actttgttgc tgtctggctt tcttccccgt attgctgttg taataagagt
     4141 tttctttgat tccgatagcc tgtcctgtac gcttttgttt gcatatgctc catcggcaat
     4201 aagttcttcc gtgtcctccc ccactattgc ttcaaaattg tcttcaagta ttgtactgtc
     4261 atcaacattg tttttttctg ttatcacatg cgtaatcacc tggagtttgt tgtctggatc
     4321 gcatgtctct actattgaaa ccgaataccc ctgacttgcc tgcttccctt tttcccgata
     4381 tgtcgcatca ggatcatacg gtgattgtat gcaactgctc cttagttcgt ttgaaacttt
     4441 tacctttacc cctttccctt tccctgttat ctctatctgc tcatcaacca gccgtgcaag
     4501 tattcgatat gcctggcttt cactcacaga atcatgggca aatagcacct tgatacggta
     4561 aagatactgt gcaatctcct gaattttcat ccttgcatcg tcattcgtca gagaaaatac
     4621 gtaattctca ggtgttatat ccttatatac ctttatctct tccgataacc tctccaaaat
     4681 actctcatca ctatgcttca aaaaataatt caacacctct aaaaacagtt tgagccggtt
     4741 tagtctgcga atatttgaac caattaacgt tgaatcaatc ctcttgactt tctgactaat
     4801 cccaaatttc tttatataat catccctcaa atgtacaaat actttctcaa atagatttat
     4861 ccctgtcttt tcctcataat caacaacacg tcggcgcata taataaaatg ctcgttctga
     4921 tattgtcctc tcaccaatcc tgtttaaccc catcgccagt ataaacaaat gatcaaaatg
     4981 aaacctgcct aattaatttt tttaatctac atagtgttct ccagttaaaa atttaaaata
     5041 atttattata atttcctttg ccttttctaa agaaatatta tcatcatatt gaacatattt
     5101 ttttaaaaaa ttatttctca ttatacattc ataaatgaaa taatctttat atttattaaa
     5161 catttcatta tttataaata catctaagtt cccaaaataa tatggaatta aatgactaaa
     5221 ttgtttaaat acatctacat aaatataata atctaattta tttttatcta caataatagc
     5281 attataccca aattcatgga taaaagttct atttcttttt aaagcattta tatctctcat
     5341 tgtccaaaac aatatatcat tttcatttgg tttaatgttt tttaatggat atttattaat
     5401 ataattggaa ataaaatcaa aatcattcaa ggaaaagttt ttattatcaa ttgttttgaa
     5461 tttataattt tgaacataat tataatccat tccagtcttt tcttttttat ataaagctat
     5521 tattattggg aaagaaatac tttttgaagt ttctttaaat gttccactat ctataataac
     5581 tccattaact agtttataat tttttgtaaa attttttaat aaattaaaat tggatttttt
     5641 aatcaaataa gacaaagggt gtaaaacaca aattatatct gcatctaatt ttgcatatga
     5701 taacaaaaaa gacattccat aatcccttgt ttttatatca atatccattt ctaaattatc
     5761 attttttata cctcttctta ttattgaagt agtatcatta taagggggtt tccaataata
     5821 attaattcat tattatcaga gatattaaac atttttctat tcacatttaa taaagcattt
     5881 ttattataaa catcaatttc agggaaatta tcttttgttt gattagtagc aatataatca
     5941 atatcatttg cctttttcgg acaattaatt ttataatcaa aaaaatttcc atatccacaa
     6001 gagctatcta aaacaatact tttattattt ataaaaggtt ttatcatttc ccatactata
     6061 tcaacatatt tcgttggagt ataaaaagca ccaagattta ttttatcata gtactttaaa
     6121 tgatattgat ttttaatatc aatacctttt ttattttctt gaatctgtat atctgttatt
     6181 gtcatattat ctccttaata
//
LOCUS       DAOSOX010000141         6197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12750_length_6197_cov_8.588733, whole genome shotgun sequence.
ACCESSION   DAOSOX010000141 DAOSOX010000000
VERSION     DAOSOX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12750_length_6197_cov_8.588733"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="hslU"
                     /locus_tag="PLX22_09440"
     CDS             complement(<1..988)
                     /gene="hslU"
                     /locus_tag="PLX22_09440"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOT20160.1"
                     /translation="MSDIIQFMENHDLTPRQIVSELDKYIIGQKDAKKCVAIALRNRS
                     RRKKLDPELQEEISPKNIIMIGPTGVGKTEIARRLSKLVNAPFIKVEITKYTEVGYVG
                     RDVESMIRDLIAIAVNMVKKEYSTMVEEKALKNAYDKIVDIIIPKKGFEFDDRQVSGF
                     HMERESIFESDTMYEQMRKQYLEDLKNGNLDDMEIEYESEMPNVIPVMSVMGGSGLED
                     IDMQIQSMLGDLMPKKSKRKKSTVKDALRIFITEETEKLIDMEKVTIEAVKRVEDMGI
                     VFIDEIDKIAGAESKSGPDVSRQGVQRDLLPIIEGTTVNTKYGPIKTNHILFI"
     gene            complement(988..1533)
                     /gene="hslV"
                     /locus_tag="PLX22_09445"
     CDS             complement(988..1533)
                     /gene="hslV"
                     /locus_tag="PLX22_09445"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOT20161.1"
                     /translation="MNNVTYRGTTVLAIKKDNTLAMGCDGQVTLGDTVIKSQAVKLRK
                     VYNDKVMTGFAGSVADALTLFERFEQKLESYSGNIPRSVVELARDWRLDRALRRLEAV
                     LIVGDPQHLYLISGNGEIIEPDDGILGIGSGGNYALAAARALLRNTSLDALEIVKQSL
                     LIASEICIYSNSNIVIEEIKQ"
     gene            complement(1526..2434)
                     /locus_tag="PLX22_09450"
     CDS             complement(1526..2434)
                     /locus_tag="PLX22_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT20162.1"
                     /translation="MDHYIDTFIRYLISEKNASPLTVESYSKDLKQFVEAVHNYLSTD
                     DIDIKGITAEHIRQFIDICFENGNELSTIGRKIATLKSFFRYMEFHNYISINPARDIH
                     FPKQNNYLPVFLTFDQIQKLTTFQCHNFIDFRDKALLEVFYSTGARVSEIANANIVDL
                     DCEKKTLKVLGKGSKERIVFLTDDTVYWFDKYYNCRLQKFGIMTDPLFINNSGKRLTS
                     RGIFYIIEKRANMAGLWKSVSPHVLRHSFATELLNRGADIKAVQDMLGHENISTTQKY
                     THTTIKRLKELYQKCHPHAIRRDNNE"
     gene            complement(2436..4538)
                     /gene="topA"
                     /locus_tag="PLX22_09455"
     CDS             complement(2436..4538)
                     /gene="topA"
                     /locus_tag="PLX22_09455"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT20163.1"
                     /translation="MADHKKTLVIVESPTKAKTINKYLGKDYMIASSMGHVIDLPKSR
                     IAVDVDNDFKPDYITIRGKAKLIKDLKKKALEAKNVLLATDPDREGEAISWHLSNVLK
                     DKNRDIKRIEFNEITEEAIKEAVKNARDINMDLVNAQQARRILDRLVGYYLSPILWKK
                     VKKGLSAGRVQSVALRVICEREKEIENFIPQEYWTLDAIMEHNKSEFIASLVAEDGNR
                     IDLKTKADVDRILSLIERKPFTVKKIEQKERRRKPQPPYITSKLQQDAARRLGFSASK
                     TMMIAQQLYEGVKLSGEGQVGLITYMRTDSTRVAHTAVNAVREYLQKYYSKDYISDVP
                     NIYPNKKGAQDAHEAIRPTNVFRTPESIKNDLTPDQYKLYSLIWKRFVSSQMTPEVSQ
                     VISVIFDVVNFEFRSSGSKVLFDGFTAIEKEESAQKKLLPALEVGQVLMPKSFSPEQH
                     FTTPPPRFNEASLIKFLEESGIGRPSTYAPIINTLFKRYYVVKRGKQLVPTVLGRLVN
                     DIMVSYFESLINIDFTAKMEEQLDMIEEAHNDWVSMIREFYDPFKATVSHAEEHIGDM
                     KNILDEETDLVCEKCGKKMVKRLGKFGFFLACSGFPQCTNAKPLPLGKCPKCGGDIVK
                     RSGKRRGSFYACNRYPECDFATREEVSERSCPQCGSVLFIRKIKKKGEMLVCLNEQCG
                     YSVTLLDDDKTETEVNVS"
     gene            complement(4617..5225)
                     /locus_tag="PLX22_09460"
     CDS             complement(4617..5225)
                     /locus_tag="PLX22_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20164.1"
                     /translation="MIVKKILIVMLMVLWCISFVQIFSVPPYSGDIVKIYKSGYFKEL
                     ERNMFLITDSFLQIKTMSKAEYAWIYLNDISTKYNIIINVYNSEGRLVKAPGMSQIGN
                     DTKVIPLLHELKPEAKFFITGDSYCGIIPVYRKSECNFCHQVSQQPLIGIITYSMPYD
                     GYIYYTSERIIIFVIITFAISCLLFVVIRWQPYKDIQELFDK"
     gene            complement(5233..>6197)
                     /gene="dprA"
                     /locus_tag="PLX22_09465"
     CDS             complement(5233..>6197)
                     /gene="dprA"
                     /locus_tag="PLX22_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT20165.1"
                     /translation="ATWYSGTPLEESEKIIELCHKLNVTIISYWDDAYPQLLREIVYP
                     PAVIYCKGILPNKPYIAIVGTRNADPKSAFIARTLSGTLASAGIAVVSGLAVGIDREA
                     HSGALKANGSTIGVMANGIDMLYPASNRDIYTDIINSGTGCVLSEYPPKVRINKWTFV
                     RRNRIVSGLSRATIIVKAAQKSGALITARYALEQNRELFVCPGHSFDEGYKGCFMLLK
                     EGAHPVYDENDIFEVLNIGSTRKEMAIMKNTNIDDTNMMESNLFAVYDNDDIVKTLRQ
                     GAMEIDSLIRLMGKSSAEVLEKLTTMELQGIITRSGNIIALNNK"
BASE COUNT         1861 a         1323 c          942 g         2071 t
ORIGIN      
        1 caataaatag tatatgattt gtttttatag gaccatactt ggtatttacc gttgtacctt
       61 caataatggg taataaatca cgctgtaccc cctgccgtga aacatcaggc cctgattttg
      121 attctgctcc agcaatttta tcaatttcat caataaaaac tatacccata tcctctacac
      181 gtttaacagc ttcaatagtg accttttcca tgtctataag cttttctgtt tcttcggtta
      241 tgaaaatcct gagtgcatct ttaacagtac tttttttccg ttttgatttt tttggcataa
      301 gatcgcccag catggattgt atctgcatat caatatcctc aagtccggaa ccacccataa
      361 ccgacataac cgggataaca tttggcattt cagactcata ttcaatttcc atatcatcaa
      421 gatttccatt ttttaaatct tcaaggtatt gtttcctcat ctgctcatac atggtatcag
      481 actcaaaaat agattcgcgc tccatatgaa atccagaaac ctgcctgtca tcaaattcaa
      541 aacctttttt agggataatt atatcaacta tcttatcata ggcattcttc aacgcctttt
      601 cctcaaccat tgtgctatat tcttttttta ccatattgac agcaattgct attaaatctc
      661 gtatcattga ttcaacatca cgacctacat atccaacctc agtatattta gtaatttcaa
      721 cttttataaa aggagcatta acaagtttcg atagtcgccg ggcaatttcg gttttgccaa
      781 caccggtagg tcctatcatg atgatatttt taggcgatat ttcttcctgt aattcgggat
      841 ctaacttttt acgacggctt ctgtttctga gagctatcgc cacacatttt ttagcatcct
      901 tctgcccaat aatatattta tccagttcac tgacaatttg acgaggagtt agatcatgat
      961 tttccataaa ctgtatgata tcactcatta ttgttttatc tcctcaataa caatattgct
     1021 attggaataa atgcaaattt ccgaggcaat caataacgat tgtttcacta tctcaagagc
     1081 atctaatgat gtatttctga gcagtgccct ggctgctgcc aatgcataat taccacccga
     1141 accaatgcct aaaatgccat catccggctc tataatctca ccatttccgc ttataagata
     1201 aagatgctga ggatcgccaa caatcagtac agcctccagc ctgcgcaggg ctctgtccag
     1261 ccgccagtca cgcgccagct caacaaccga acgcggaata tttccggaat acgactccag
     1321 cttttgttca aagcgttcaa aaagggttag tgcatcagca accgaaccgg caaaaccggt
     1381 cattacttta tcattataca ctttgcgtaa tttaaccgcc tgacttttta taacagtatc
     1441 acccaatgta acctggccat cacatcccat ggcaagggta ttgtctttct ttatagcaag
     1501 aacagtggtt ccccggtatg taacattatt cattgttgtc tctcctgatt gcgtggggat
     1561 gacatttttg atataattct tttaatcgct taatggtagt atgagtatat ttctgcgttg
     1621 ttgaaatatt ctcatgtcct aacatgtcct gcacagcttt aatatccgca cctctgttaa
     1681 gcagttcagt ggcaaagcta tgacgcaaaa catgagggct aaccgacttc cacaaacctg
     1741 ccatgtttgc ccttttttca ataatataaa agataccacg cgatgtcaac cgtttaccac
     1801 tattattaat aaataatgga tcggtcataa taccaaattt ttgtaagcga cagttgtaat
     1861 atttatcaaa ccagtaaact gtatcatcag tcaaaaaaac aattctttct tttgaacctt
     1921 tacccaacac cttcaatgtt tttttttcac aatcaaggtc aacaatgttt gcattagcaa
     1981 tctcggaaac acgtgcgcca gttgagtaaa atacctcaag caatgcctta tcacgaaaat
     2041 caataaaatt gtgacattga aatgttgtaa gtttttgtat ctgatcaaag gttaaaaaaa
     2101 cgggaagata gttattttgt ttagggaagt gaatgtcccg tgcgggatta atacttatat
     2161 aattatgaaa ttccatatac ctaaaaaaag atttcaatgt tgctattttt cttccaatag
     2221 tagacaattc atttccattt tcaaagcaga tatcaataaa ttgacgaatg tgctcggcgg
     2281 ttatgccttt gatatcaata tcatctgttg ataaataatt atgaactgcc tcaacaaatt
     2341 gttttaaatc tttactatat gattcaaccg ttaatggcga tgcatttttc tcagaaataa
     2401 ggtagcgtat aaatgtatca atataatgat ccatatcaac ttacatttac ttctgtttca
     2461 gttttatcat cgtccagcaa tgttaccgaa taaccacact gctcatttaa acatacaagc
     2521 atttcgcctt ttttctttat cttgcgaata aacaatacgc taccgcattg tgggcatgaa
     2581 cgctctgata cctcttcacg ggttgcaaaa tcacattcag ggtatcggtt acacgcataa
     2641 aatgaaccac ggcgcttccc cgaacgtttt acaatatcac cgccacactt tggacatttc
     2701 cccaacggca atggttttgc attggtacac tgcggaaatc ctgagcaggc aaggaaaaac
     2761 ccaaatttac ccaaacgttt aaccatcttt ttgccacact tttcacatac taaatcagtt
     2821 tcttcgtcca ggatattttt catatcccct atatgttctt cggcgtgact tactgttgcc
     2881 ttaaatggat cataaaattc acgtatcatg ctaacccaat cattatgagc ctcttctatc
     2941 atatctaatt gttcttccat ctttgcggta aaatcaatat tgattaacga ttcaaaatac
     3001 gataccataa tatcgttaac cagtcttcct aaaactgttg gtaccagttg tttcccacgt
     3061 ttaaccacat aataccgttt aaacagggta ttaataatag gcgcataggt tgatggtcta
     3121 ccaatccctg attcttctaa aaactttata agtgatgctt cattaaaacg tggtggtggt
     3181 gttgtgaaat gttgttcagg gctaaacgat ttgggcatta aaacctgtcc aacctctaaa
     3241 gcaggcaaca gttttttctg ggcactttct tctttttcaa ttgcagtaaa cccatcaaac
     3301 aacaccttac ttcctgaaga cctgaattca aaatttacaa catcaaatat aacactaatc
     3361 acctgtgaaa cttcaggggt catttgtgaa gaaacaaatc gtttccatat aagtgaatat
     3421 aatttatatt gatctggcgt taagtcattt tttatagact caggtgttct gaacacattg
     3481 gttggacgta ttgcttcatg agcatcctga gctccttttt tattgggata tatatttggg
     3541 acatcagaaa tatagtcttt agaataatac ttttgcaaat attcacgaac agcattgact
     3601 gcagtatgag ctactcgtgt ggaatcagta cgcatgtaag taattaaacc aacctgcccc
     3661 tcaccggata attttacacc ttcatataac tgctgtgcaa tcatcatggt tttacttgca
     3721 gaaaatccca atcggcgggc agcatcctgt tgcaacttgc tggtaatata aggtggctgc
     3781 ggttttcgtc ttctttcctt ttgttcaatt tttttaacag tgaaaggttt cctttcaatt
     3841 aaggataata tcctgtcaac atcagctttt gtctttaaat caatcctgtt accatcttca
     3901 gctacaagtg aagcaataaa ttctgatttg ttatgctcca tgatagcatc taatgtccag
     3961 tattcctgtg gaataaaatt ttctatttct ttctcacgtt cacagattac cctcaatgca
     4021 accgattgca cacgacctgc cgataatcct ttttttactt ttttccataa tattgggctt
     4081 aaatagtaac caaccagtct atcaagaata cgccgtgcct gctgtgcatt aaccagatcc
     4141 atattaatat cgcgtgcatt cttcactgct tctttaattg cttcttcagt aatttcatta
     4201 aattctattc gctttatatc tctgttttta tcttttaaaa catttgatag atgccaggaa
     4261 attgcctcgc cttctctgtc agggtcagtt gcaaggagaa catttttagc ctcaagggct
     4321 tttttcttaa gatcttttat taatttagcc ttgccacgta ttgttatata atcaggttta
     4381 aaatcattat ccacatcaac ggctatacgt gatttgggaa gatcaatgac atgccccatt
     4441 gacgaggcaa tcatataatc cttgcctaaa tatttattta ttgttttagc tttcgtaggt
     4501 gactcaacta taacaagtgt tttcttatga tcagccatat tattttcctt ttaatacatt
     4561 aaaataagcg atagttcatg gtattattga ttgcacatca tgaaaatagc tactcattat
     4621 ttgtcaaata attcctgaat atccttatac ggttgccatc gaatgactac aaaaagcaaa
     4681 caacttatgg caaacgttat aataacaaat ataataatcc gttctgatgt ataatagata
     4741 tagccatcat acggcataga gtaggttata attccaatta aaggttgctg tgaaacctga
     4801 tgacaaaaat tgcattcgga ttttctatac acaggaatta ttccacaata actatcgccc
     4861 gtaataaaaa attttgcttc aggtttaagc tcatgcagca atggtataac cttagtatca
     4921 ttaccaatct ggctcattcc tggtgctttc accaatctgc cctcagaatt ataaacattg
     4981 ataataatat tatactttgt tgatatatca ttcaaataaa tccatgcata ttctgctttt
     5041 gacatggttt taatctgtaa aaaagaatcg gtgatcaaaa acatattcct ttcgagttct
     5101 ttgaaataac ctgatttata tattttaact atatcgccac tatacggtgg aacagaaaaa
     5161 atctggacaa aacttataca ccagagcacc atgagcataa ctattaatat tttcttaact
     5221 atcatacaat ctttatttat tgttcaaggc tattatattc ccggatcgtg taataatacc
     5281 ctgcagttcc atagtagtca atttttcaag aacttcagcg ctgcttttac ccatcaaacg
     5341 gattagtgaa tcaatttcca tagccccctg cctcaatgtt tttacaatat catcattgtc
     5401 ataaacagca aacaaattgc tctccatcat attagtatca tctatattgg tattcttcat
     5461 tatagccatt tcttttctgg tgctgccgat atttaaaact tcaaagatat cattctcatc
     5521 atagacagga tgtgcacctt ctttcaatag catgaaacaa cctttatacc cttcatcaaa
     5581 agaatgtccc gggcaaacaa acagttccct attctgttcc aatgcatacc gggcagtaat
     5641 aagagctcca cttttctggg ctgctttaac tatgatggta gctcgagata aaccactgac
     5701 tattctattc cgccttacaa aggtccattt gttaatccgt acctttggtg gatattctga
     5761 caatacacat ccagtgcccg aattaataat atccgtgtag atatccctgt tgcttgcagg
     5821 atacaacata tctattccat tagccataac accaatggtt gagccatttg ctttcaatgc
     5881 accactatgc gcttctctat caattccaac tgccaaccca ctgacaacag caatacctgc
     5941 tgaagcaagt gtccccgata aagtacgtgc aataaaggct gacttaggat cagcattcct
     6001 ggtacccact attgcaatgt atggtttatt gggcaatatg cctttacaat atataacagc
     6061 aggaggatag actatctctc gcaaaagttg tggatatgca tcatcccagt atgatataat
     6121 agtcacatta agcttatggc acaattcaat aatcttttct gattcttcca gtggcgtgcc
     6181 actataccac gtagcat
//
LOCUS       DAOSOX010000142         6187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_12773_length_6187_cov_7.305284, whole genome shotgun sequence.
ACCESSION   DAOSOX010000142 DAOSOX010000000
VERSION     DAOSOX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12773_length_6187_cov_7.305284"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..1717
                     /locus_tag="PLX22_09470"
     CDS             458..1717
                     /locus_tag="PLX22_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOT20166.1"
                     /translation="MNHYKQMYITIRCITLPIILCLSIIALPSCSDSTNGYVPSWTIM
                     VYMDGDNNLERYALADFNEMEQVDTNGHSINVIVLLDRCDGETTADGDWKGTRLYKIL
                     HDTNSSKINSERLADSRYLKITNIGDSDELNMGNPDTLSNFISFGKENYPADYYSLIL
                     WDHGSGWRSTNSTALIASEPQTFKAVCVDATSANDILEMKEVKQALLGKGLTLIGFDA
                     CLMSMAEVAYQLKDCARYMVASEDLEPASGWLYSNFLNYFIASGRSPIDLGQSIIDAY
                     ALYGSYPMSMVDLSNIDVMTGAIDSLSSALITHIDAIKDTISTARDKTLAFQSSSSPP
                     YAYIDLYDFLLNISSVNEVSYEVNDAMNAVKSAVLYHYRGDYTHSNGLSIYFPQSDTD
                     SEYSSYNTYTIDFAQTLWDDFLAEYFQ"
     gene            complement(1720..2733)
                     /locus_tag="PLX22_09475"
     CDS             complement(1720..2733)
                     /locus_tag="PLX22_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20167.1"
                     /translation="MNKEELMISANYVRYNPDNKGHYESFFIRANHPQKPLAFWIRYT
                     IFQPAKNHRNALGELWAIVFDGEGHHRALKAEYPISQCSFAPDDFNIVIGDAVLMPGI
                     AYGCIAYGDETIQWDVTYSGNSDPLFLYPAKLYTAKLPKAKTLVGLPFARFNGSIQIN
                     GETIEIMNWVGSQNHNWGSKHTDHYAWGQVCGFDNEPDAFLELATARIKVGPLFTPFM
                     TPLVLRYKGNEYALNSLSKLLCRARFGYFYWDFKAVEKNSTVKGSIVALKEDFVCLPY
                     YNPPGGIKYCLNTKIASCEITCKVGNDQWTLKTKNRCAFEILTDDTMHGLQPIREIAV
                     IKN"
     gene            complement(2806..3249)
                     /locus_tag="PLX22_09480"
     CDS             complement(2806..3249)
                     /locus_tag="PLX22_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20168.1"
                     /translation="MNKKIIRLITVTGLLYTIAFTVTAFVFIFLHPQLMAIINAVSQK
                     IFPALPLAHEQSQFYKILCLSMMSGVTVCSLLLYKDAQTYIAMVIPLCTMKFTSSLCG
                     LTAFIGGSVFHNGWNNLANIIIVITDFPLGLWIFCLFIVYRRYNN"
     gene            complement(3441..4100)
                     /locus_tag="PLX22_09485"
     CDS             complement(3441..4100)
                     /locus_tag="PLX22_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20169.1"
                     /translation="MKMKKVMVTVCLLLFMSMNIFYDAYPDTLPGLTHDDIQKLQAGT
                     IIITQQKQNTADSQIARVTGAICIHKPINEVWKCLVDWETMPRYVDSLDYYKVIAKMT
                     DDVWIIEGQLHVTFIKFRYTLIVNSNKSKYYQQWRLLQHNDIAQYNLQNKTNPNSGGI
                     KNIEGYQYCIPYDDTTTILYYAPVVAVSVPVPGFIEDSLTKKSIRDYLYGIKNYLEKT
                     Q"
     gene            complement(4100..5608)
                     /locus_tag="PLX22_09490"
     CDS             complement(4100..5608)
                     /locus_tag="PLX22_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOT20170.1"
                     /translation="MIYSYSDYEHSKQLSADVCVIGSGAGGAVAAYELAASGYRVIIV
                     EEGGFYSTKDYDGKPLTSMKAMWRGAGATVSFGIPSISIPAGKCVGGTTAINSATCFR
                     TPEHIVHQWKQRLQVNLDYKELETYCERVETIINVTKLPWDVLGNCANIVKRGADALG
                     LHCKPLMHNVKNCKGCGTCQFGCVEGAKQSMEVSYIPLALQYNAMLLTQCKAVKILFK
                     GNKAYGVQCAITNEAGRKATCTINASVIVCACGSMLTPLLLKQSGVRNKHLGKHLQIH
                     PCSRVVALMDEEVKGWIGVSQGAYIDDYADEGIMLEGIFVHPSILAASLPGVGNDFLQ
                     LVQRFNNIAAFGVMVHDTAEGVVSGNLNTPLLWYSMSGKDIETLKKGIAYLSMVFIAA
                     GAKQVFTPLSPFPVLHSADDVKKLEHAHITRKDIPEVFAFHPLSTCRMAGDSSCGVVD
                     SYGKCFNTDNVYIADGSIVPTSLGANPQITIMTLATKISRHIADTLAHTRCV"
     gene            complement(5618..5995)
                     /locus_tag="PLX22_09495"
     CDS             complement(5618..5995)
                     /locus_tag="PLX22_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20171.1"
                     /translation="MLFNNSLIIKSTITAAKSILCRDDFVHEDFFMALQQYLALTPFY
                     VRWIYSFGMYYCNLLSLIKQHTLFYKLPLDKSIQLLHSMEKGNLFINRSLIMMLKLFT
                     TMVYFDDDVHAQLIGYEHLAHCQ"
BASE COUNT         1901 a         1271 c         1087 g         1928 t
ORIGIN      
        1 ttcttggctg tttccaatga ttctataaac tgtgatatat agctattata ttcatgggta
       61 gtatacaggc ttataatacc tgataaaaca gttattataa taaaaggaat aaaaatatat
      121 attatattaa aatgtttaat tagtaaacgc ttgatattaa tcataaacca caaccatgcc
      181 attaatattt ttatataaat tttagtattg aatttaaaga ttaatgaatc aacacgtgtt
      241 taaaaaatta tgaagatagc ataattatat tgtaaaattt actattacat gccaataaaa
      301 ccattaatat aaattgatac aaagtagata gttgttgctt gataacaaca aggataccat
      361 tacattgtat agtagtaatt tataacctga atcagaaaaa tgattgccta tatactggat
      421 atacaaccta ctatgtttat acaaacaggg atataccatg aaccattata aacaaatgta
      481 catcaccata cgttgcataa cactgccaat catactttgc cttagcatca tagcattacc
      541 ttcatgcagt gacagcacta acggctatgt gccatcatgg actatcatgg tatatatgga
      601 tggtgataac aaccttgaac ggtatgcact tgctgatttt aatgagatgg aacaggtgga
      661 tacaaacggg catagcatca atgtcattgt cctccttgac cgttgtgatg gtgaaacgac
      721 agccgatggt gattggaaag gcactcgtct gtataaaatt ttgcatgata ccaacagctc
      781 aaaaataaac tctgaaagat tagccgacag ccgctattta aaaataacaa acatcggcga
      841 tagcgatgaa ttgaacatgg gaaatcccga tactttaagt aattttattt cttttggtaa
      901 agaaaattat ccggcagatt attatagcct tatactatgg gaccatggtt caggatggcg
      961 cagcaccaat agcactgctt tgattgcatc tgaacctcag acatttaaag cagtatgtgt
     1021 ggatgctaca agcgctaacg atattttgga aatgaaagag gtgaagcagg cattgcttgg
     1081 aaaagggcta acgctcattg gttttgatgc atgccttatg agtatggcag aggttgccta
     1141 ccagcttaaa gattgtgcgc gctacatggt tgcatctgaa gatttagaac ctgcaagtgg
     1201 ctggctctat agcaacttcc ttaattattt tattgcatcg ggcagaagcc ccatcgattt
     1261 aggacaatca attattgacg cttatgccct ttatggttcc taccccatgt caatggttga
     1321 tctcagtaac attgacgtta tgactggtgc cattgattca ttatcatcag cattaataac
     1381 acatattgat gctattaaag ataccattag cacagcacgt gacaaaacgt tagcatttca
     1441 aagttcatca tcaccaccgt atgcatacat cgatttgtat gattttttgt tgaacatatc
     1501 atctgttaat gaagtttctt acgaagtaaa cgatgcaatg aatgccgtga aaagtgctgt
     1561 gctctatcat taccgtggtg attacacaca ttcaaacgga ttatccatct attttccaca
     1621 atcagatacc gatagcgaat acagcagcta caatacctat actattgact ttgcacaaac
     1681 cctgtgggat gatttcctgg cagaatattt tcagtaaaat caatttttaa taactgctat
     1741 ctccctgatt ggctgtaaac catgcatggt atcatcagtc agtatttcaa aggcacatct
     1801 attctttgtt tttaaggtcc attgatcgtt gcccacttta caggttattt cacatgatgc
     1861 aatcttggtg ttgaggcaat actttatacc accaggtgga ttgtaatagg gaagacatac
     1921 aaaatcttct ttcaatgcaa caattgaacc tttcacagta ctattttttt ccactgcttt
     1981 aaagtcccaa taaaaataac caaaccgtgc cctgcatagc aattttgata acgaattgag
     2041 tgcatattca tttcctttgt atcgtaacac cagtggcgtc ataaacggag taaagagggg
     2101 ccctaccttt atgcgagctg ttgccaattc cagaaaagcg tctggctcat tgtcaaagcc
     2161 acaaacctgc ccccacgcat agtggtcggt atgctttgaa ccccagttat gattttggct
     2221 tccaacccag ttcattatct ctatcgtttc accatttatc tgtatagaac cattaaaccg
     2281 tgcaaatggc aaacccacca gcgtttttgc tttgggaagt tttgcagtgt aaagttttgc
     2341 cgggtacaaa aaaagcgggt cgctgttccc tgaataggtt acatcccact gtattgtttc
     2401 atcaccatac gcaatgcacc cgtaggctat tccaggcatt aaaactgcat ctccaatcac
     2461 aatattgaaa tcgtccggtg caaaactgca ttgtgaaatt ggatactcag cctttaatgc
     2521 tctatgatga ccttctccat caaaaactat cgcccacaat tcccctaaag catttctatg
     2581 attttttgca ggctgaaaga tggtatagcg tatccagaac gctaatggct tttgaggatg
     2641 attggcacgg ataaaaaaac tttcgtaatg acctttgtta tcgggattat agcgtacata
     2701 gttggctgaa atcataagct cttccttatt cataaagcct cctgtttgtc gttatatgct
     2761 aaaaatattt tttggcgata gttactgtta atcacaattt atgcattaat tgttataccg
     2821 cctgtatact ataaacaagc aaaaaatcca tagccctaaa ggaaaatctg ttataacaat
     2881 tattatattt gccagattgt tccagccatt gtgaaaaaca cttcccccta taaatgctgt
     2941 taatccgcat aatgacgatg taaatttcat agtgcacagt ggtataacca tagcaatata
     3001 tgtttgcgcg tctttataca agagcagtga acagacagta actcccgaca tcattgataa
     3061 acataagatt ttataaaact gactttgttc atgtgccagt ggcagtgccg ggaaaatttt
     3121 ttgcgacact gcattaataa ttgccatcag ttgtggatgt aaaaatataa aaacaaatgc
     3181 tgtaaccgta aacgctattg tgtaaagcaa ccctgttacg gtaatcaaac ggatgatttt
     3241 tttattcata aaaaataatc ctccaatcat gtgatgattc cctcaatgtc attacgggct
     3301 tttccagaaa atgatgtatt gtttttgtgg gcgtatcgca atacgccctt actatagtac
     3361 aatacaatat cccctgcagg gttcttattc ttttgtatca tgtttggcta accataatcc
     3421 gactattgtt gaatgctata ttattgtgtt ttttcaagat agttcttaat accatacaga
     3481 taatccctga tacttttttt ggtgaggcta tcttcaataa aacccggtac cggcactgat
     3541 actgcaacaa ccggagcata atacagtatt gttgtagtgt catcataggg aatacagtac
     3601 tgataccctt caatattttt tataccaccg ctgtttggat ttgttttatt ttgaagatta
     3661 tactgtgcaa tatcattgtg ctgaagcagt cgccattgtt gataatactt acttttattg
     3721 ctgttgacaa tgagtgtata tctaaatttt ataaatgtaa catgcagttg tccttcaatt
     3781 atccacacat catcagtcat ttttgcaata actttataat aatccaggct atcaacatag
     3841 cgtggcattg tttcccagtc aacgaggcat ttccacacct cattgatagg tttatgtata
     3901 caaatagccc ctgttacacg ggcaatctgg ctatcagcag tattttgctt ttgctgtgtt
     3961 ataatgatag taccagcctg cagcttttgt atatcatcat gggttaaacc tggcagcgta
     4021 tccggatagg catcataaaa aatattcatt gacataaata gtagcaaaca tactgtcacc
     4081 atgacttttt tcatcttcat cacacacacc gtgtatgagc taatgtatca gcaatgtgcc
     4141 gcgaaatctt tgtagctaaa gtcataatgg taatctgtgg atttgctccc agtgaagttg
     4201 gcacgatgct gccatcggca atatatacat tatcagtgtt aaaacattta ccataactat
     4261 cgaccactcc acaggaacta tcgcccgcca tacgacatgt actcaatgga tgaaaggcaa
     4321 aaacctccgg gatatctttc cgcgtgatat gggcatgttc caatttttta acatcatccg
     4381 ctgaatggag caccggaaat ggcgatagcg gcgtaaagac ctgttttgct ccggcagcga
     4441 taaaaaccat ggacaggtat gcgatgcctt tttttaaagt ttcaatatct ttgccactca
     4501 tggaatacca taaaagtggc gtattcaaat tccccgatac aacgccctcg gcagtatcat
     4561 gcaccattac cccaaacgct gcaatattgt taaaccgctg caccaactgt aaaaaatcat
     4621 ttcccacacc gggaagtgat gcagccaata ttgagggatg tacaaaaatg ccttccagca
     4681 taataccttc atctgcataa tcgtcaatat atgctccctg cgaaacgcca atccagcctt
     4741 taacctcttc atccatgaga gcaacaacac gggagcaggg atgaatctgc aaatgtttac
     4801 caagatgttt gttgcgtacg ccactttgtt taagcaacag cggagtaagc atgctgccgc
     4861 atgcacaaac aataacagaa gcattaatag tacaggtagc tttcctccct gcttcattgg
     4921 ttatggcaca ttgaacacca tacgctttat tccctttaaa aagaattttt accgctttac
     4981 actgtgttaa tagcattgcg ttgtattgta aggctaaagg aatatagctt acctccatac
     5041 tttgtttggc tccttcaaca cagccaaact gacaggtacc gcaaccctta caatttttta
     5101 cattgtgcat taacggtttg cagtgcaatc caagcgcatc tgcaccccgc ttaacaatgt
     5161 ttgcgcaatt gccaagcaca tcccatggca gtttcgtaac attgattatt gtttcaacac
     5221 gctcacaata tgtttcaagt tctttataat caagatttac ctgtaaccgt tgtttccatt
     5281 gatgaacaat atgctctggc gttctgaaac atgttgcaga attaatggct gtagtgccac
     5341 caacacattt gcctgctgga attgatatgg acggaatgcc aaacgaaaca gtagcacctg
     5401 caccacgcca cattgctttc atactggtaa gtggcttgcc gtcataatct tttgttgaat
     5461 aaaaaccgcc ctcttcaaca atgataacac gataccctga tgcagcaagc tcataggcag
     5521 ccactgcccc accagcaccg ctgccaatga cgcaaacatc agcgctcaat tgtttgctgt
     5581 gttcataatc gctgtatgaa tatatcatat catgcctcta ttggcaatgt gccagatgct
     5641 catagccaat caattgtgca tgcacatcat catcaaaata taccatcgtg gtgaataatt
     5701 ttagcatcat tatcaatgac ctgttgataa acaaattgcc tttttccatg ctatgcagaa
     5761 gctgtattga tttatccagt ggcagtttat aaaaaagtgt atgttgtttt ataagtgata
     5821 gcagattgca ataatacatg ccgaaactat atatccaccg tacataaaaa ggggtcagag
     5881 ctaaatattg ttgtagtgcc atgaagaaat cctcatggac aaaatcatct ctgcatagta
     5941 tgctttttgc tgctgttata gtacttttta ttatcagcga gttattaaaa agcatggtaa
     6001 gtattcctcc caataatata gactttaagt acatattatt ggggctatat attatgtcaa
     6061 catatatttt tattttggta ctttttttaa ttattataaa gctctgaccc cttttttata
     6121 cccactgtgc aatggagtag cgcttacggt acgcctcctc atactgtaaa aaacgcctca
     6181 cccgttc
//
LOCUS       DAOSOX010000143         6042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13225_length_6042_cov_7.309170, whole genome shotgun sequence.
ACCESSION   DAOSOX010000143 DAOSOX010000000
VERSION     DAOSOX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13225_length_6042_cov_7.309170"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLX22_09500"
     CDS             complement(<1..908)
                     /locus_tag="PLX22_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20172.1"
                     /translation="MTKENGNKPYALILAGYDKPDRSTMIKRKKQIIEAYDGDSIYIG
                     KNKFLQQLAGKPVIQYVLDAVYNAKKKGKPLYDAIYVYNDVKNFTDVIDTRKYHNLHV
                     MQMKQSVGGHLKDFYFNHLEYGRRVDIFFGDTPRITSEDVEWINSQYDTILGKAKDYR
                     GITAHVAYGIVEYEDLKDDTWLNHRLKIIKRGQNKGKLKNFVGFTTFEARVGNSAALL
                     KHRSLDSAVEYEIIDFFYSLRKALAPNIFSKIIYYLWKTKNFILIRQIKRHCIEEERF
                     YKAAINVIERVYNLDLSKFGGYIFHI"
     gene            1097..>2418
                     /locus_tag="PLX22_09505"
     CDS             1097..>2418
                     /locus_tag="PLX22_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT20173.1"
                     /translation="MKKYVIFILIAFVACATGKNTAHTKSLSKELKSLVSQSCFEVVV
                     NKPEKDTLTYEKELPWHLIPYNIRVDKYYSIGTAFAVSPTELLTAFHVLDLKTDSLIY
                     KDFYIRDTQGNVYEIDKILAFDSHRDFIRFTVKNKTFTKYLKLKEEHVVGDTVYAVGN
                     AYGEGIVMRQGELIGTYPEPEDNRFKLLKSSSDVNFGNSGGPLVDDDANVVGIVIQRK
                     DNIAYSLPVSEVSKTGNRGVFHVKMNFGFTLLPEKTIVKDYDDTLQLPMGYKEIKKTF
                     NQRFKKFYTDTMDQFLAQEGKGMFPNGDESLIPLFDFTDSYLLQVNYLSKDNNKWAIS
                     NIEYKETKIRNNGVIRIANPDKRLLFIDISRPDDVSMMDMLNDPKVGMRLVLEGQSIT
                     RDFGDVKTRILSLGDPIATEYHTDNYQRKWRLCIWVMEYSDEAIISCTT"
     assembly_gap    2419..2518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2519..2938
                     /locus_tag="PLX22_09510"
     CDS             <2519..2938
                     /locus_tag="PLX22_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20174.1"
                     /translation="TINVTNDSIAINNDVKMGVTFGYIRNNNAARWTIRRVMLSEDQK
                     DNYFVLINWYAPDTRLPKDYITYWDSIVKGEHPYTMKPFASNSTTKIAAVVKKIQDQS
                     EKVTKLYTLFLGKAGKVEEEKMIQDFKILMNGITINK"
     gene            complement(2982..4439)
                     /locus_tag="PLX22_09515"
     CDS             complement(2982..4439)
                     /locus_tag="PLX22_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HOT20175.1"
                     /translation="MPLKTPSDYVESIRSLNRTIYMFGQRIENPVDHPIIKPSLNAVA
                     MTYALAQKDEYKEIMTAQSHITGNIINRFTHIHQSIDDLVKKSKMGRLLGSQTGCCFQ
                     RCVGMDALNALSMTTYAIDQTYNTTYYQRFLKYLEYVQNNDLVCDGAMTDPKGDRSLP
                     PHKQEDPDLYLRVIKELQDGIIVRGAKAHQTGAINSHEIIVMPTIAMREEDKDWAVAF
                     AVPADTPGIIYIYGRQSCDTRKLEKYTIDQGNALFGGHEALIVFDDVFVPWDRIFMYK
                     EYDFAGHLVERFASYHRQSYACKVGVGDVLIGATQTIAEYNGTEKASHVKDKIIEMIH
                     LNETLYCGCIACASEGKCEQPGTYMVNTLLANVHKQNITRFPYEIARLAQDIAGGALV
                     TLPSADDLYHPEAGKWIKKYFKTKNDVPTENRIKLFRLIENITMGTAAVGYLTESMHG
                     AGSPQAQRIMIARESNVKEKQQLAKRLAQIIASEQ"
     gene            complement(4778..5515)
                     /locus_tag="PLX22_09520"
     CDS             complement(4778..5515)
                     /locus_tag="PLX22_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT20176.1"
                     /translation="MNYRTITIPYKQPVDAIVYSAHNSKGTVVYSHGLFSSKDAYKIN
                     QIAPVLCQHGYTVISFSYCYTRDYASGTPLTIELPACAHELNAVLEYAANHYNANLHI
                     VGSSMGGVIALYYCAMMPYTVSIKSVSIIATPVHLQHLVTTLAPLGINGDSVAVGEYT
                     IAASSLNQLNSIQLIHLLPRIDVPVCIIHGNNDTVVPVTHAYDIQKNLCVPHTMHIIP
                     DGDHTLTSTKHVDLILQYILSWLTMHP"
     gene            complement(5524..5979)
                     /locus_tag="PLX22_09525"
     CDS             complement(5524..5979)
                     /locus_tag="PLX22_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20177.1"
                     /translation="MKRIYRYYIIVTAVFMMIAGNSVALKFTDLHGRWKLLYRGNYGY
                     EFRFAKNYQAVCILYLQNNAVVFKGVYTLDNPQTLRINISQMKNVDNINAVYGTGGFY
                     NVKSSYFVFHAALVQKDTKKMLELKPLSIVIDGVTSEGYFEPVISLQKQ"
BASE COUNT         1834 a         1076 c         1155 g         1877 t
ORIGIN      
        1 ttaatgtgaa agatatagcc tccaaatttt gacagatcta aattatatac tcgttctatt
       61 acattaattg cagctttata aaatctttct tcttcaatgc aatgccgctt tatctgtctg
      121 ataaggataa aatttttagt tttccataaa tagtaaataa ttttagaaaa aatatttggt
      181 gccaatgctt tgcgaagact gtaaaaaaaa tcaataattt catattcaac agcactatca
      241 agacttcgat gttttagtag cgcagcagag ttgccaacgc gtgcttcaaa tgtggtaaaa
      301 ccaacaaaat ttttcaactt acctttgttt tgtcctcgtt taatgatctt caggcgatga
      361 ttaagccatg tatcatcctt taagtcttcg tattctacaa tgccatacgc tacatgtgcg
      421 gttatacctc gataatcttt tgcttttcct agtatcgtat catactgact gtttatccat
      481 tcaacatctt cagaggttat acggggggta tcaccaaaga agatgtcaac acgccttcca
      541 tactctaagt gattaaagta aaaatctttc aggtgcccgc ctacagactg tttcatctgc
      601 atgacatgca ggttatgata ttttctggta tctatcacat cggtgaagtt ttttacatca
      661 ttgtacacgt atattgcatc gtacagtggt tttcccttct tttttgcatt gtatacagca
      721 tcaagaacat actggattac aggtttcccg gccagttgct gtaaaaattt atttttccct
      781 atatagatac tatcgccatc ataagcttca ataatctgtt ttttacgctt gatcatggtt
      841 gatcggtcag gtttatcata tccagcaagg ataagcgcat agggtttgtt gccattttct
      901 ttagtcataa ttattattgt tgaatagtaa ataataaata caatgatatt atcatagcga
      961 gtatcagtca agcgtttaac agatgaaaat aaaatggggc tatcattgta atttcacttg
     1021 acagttactc tattaaaggt aagaaccaat aaacaaataa ttttaaataa tgaaatatat
     1081 ttttggagta aaaaagatga aaaaatatgt gatttttatc ttaattgcat ttgttgcatg
     1141 tgctaccggg aaaaataccg ctcataccaa atccctttcc aaagaattaa aaagccttgt
     1201 tagtcagtca tgttttgagg ttgtagtaaa caagcctgaa aaggacacgt taacctatga
     1261 aaaagaactt ccatggcatt tgattcccta taatattcgt gttgataaat attattcaat
     1321 tggcacggcg tttgctgttt cgccaacaga gcttttaacc gctttccatg tgctggattt
     1381 aaaaaccgat tcccttatct ataaggattt ttatatacgg gacactcagg gtaacgttta
     1441 tgaaatagat aaaattttag ctttcgatag tcatcgggat ttcatccgct tcacggtaaa
     1501 gaacaaaacg tttacaaaat atcttaaact aaaagaagag catgttgttg gcgatacggt
     1561 gtacgcggtt ggcaatgcat atggtgaagg catcgtcatg cgtcagggcg agcttattgg
     1621 tacatatcct gagcctgagg ataacagatt taagctgtta aagagctcat ccgatgttaa
     1681 ctttggcaat agtggtggtc ctcttgttga tgatgacgct aatgttgttg gtattgttat
     1741 acagcgtaaa gataatatcg cctattcact gccggtaagt gaggtgtcaa agacggggaa
     1801 ccggggtgtt tttcatgtaa agatgaattt tggatttaca ttgctccctg aaaaaacgat
     1861 agtcaaggat tatgatgaca cgttgcaact ccccatgggg tataaagaaa taaaaaaaac
     1921 atttaaccag cgattcaaaa aattttatac tgataccatg gatcaatttc ttgctcagga
     1981 aggcaaaggt atgtttccca atggtgatga atcgcttatc ccactttttg attttaccga
     2041 cagttacctg ttacaggtca attacttgag caaggataac aacaaatggg ctatctcaaa
     2101 tattgagtat aaagaaacaa agatccgtaa taatggtgtt atacggatag caaatcctga
     2161 taagcggttg ctgtttattg atatttcccg tcctgatgac gtaagcatga tggatatgct
     2221 taatgatcca aaggttggga tgaggctggt attagaaggg cagagcatta cgcgtgattt
     2281 tggagatgtt aaaacgcgta ttctttcatt aggagatcca atagccactg aatatcatac
     2341 cgataattat cagcggaaat ggcggctgtg tatatgggta atggaatatt cagatgaggc
     2401 tatcataagt tgtacaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac
     2521 catcaatgtt accaatgata gtattgcaat aaacaatgat gttaaaatgg gtgtaacgtt
     2581 tggttatatc agaaataata atgctgcccg atggacaata cgtcgggtaa tgctcagtga
     2641 agatcaaaaa gataactatt ttgtgcttat aaactggtat gcaccggata cacgtttacc
     2701 caaagattat atcacttact gggatagtat tgtcaaaggt gagcatccct acaccatgaa
     2761 accatttgca agcaatagta ccacaaagat tgcggcggta gtaaaaaaga tacaggatca
     2821 atctgaaaaa gttaccaagc tctatacatt attcctgggg aaggcaggca aggttgaaga
     2881 agaaaagatg attcaggatt ttaaaatcct gatgaatggg ataacaatta ataaatagtg
     2941 aacattacac gctgccttag gcagcgtgta gtaaataatt gttattgttc tgatgcaatg
     3001 atctgtgcca ggcgttttgc caattgctgc ttttctttta catttgattc gcgtgcaatc
     3061 atgatacgtt gagcctgcgg tgagcctgct ccatgcattg attcggtcaa atatcctact
     3121 gctgcagttc ccatggttat attttctata aggcgaaaca gctttatacg gttttcagtt
     3181 ggaacatcgt tttttgtttt aaaatatttt ttaatccatt tgcctgcctc aggatgatat
     3241 agatcgtcgg ctgaaggaag ggtaaccagt gcaccaccgg ctatatcctg tgctaaacga
     3301 gcaatctcgt aggggaagcg ggtaatattc tgtttatgaa cattggcaag caacgtattg
     3361 accatatagg tacccggctg ttcacacttg ccttcagatg cacaggcaat acagccgcag
     3421 tataacgttt cattaagatg tatcatttca ataatcttgt cctttacatg ggatgccttt
     3481 tcagtaccgt tgtattcagc tattgtttgc gttgcgccaa tcaatacatc gccaacgcca
     3541 accttacatg catagctctg acggtgatat gaagcaaatc gttcaactaa atggccggca
     3601 aaatcatatt ccttatacat gaatatcctg tcccatggaa caaaaacgtc atcaaaaaca
     3661 atgagtgctt catgaccacc aaacagcgca ttaccctgat caatggtata tttttctaac
     3721 ttgcgtgtat cgcacgactg cctgccatag atataaataa tccccggcgt atcagcaggt
     3781 actgcaaagg caacagccca gtctttatct tcttcgcgca tggcgatagt tggcatgaca
     3841 ataatctcat gcgaatttat tgcaccggtt tgatgtgctt tggcaccgcg tacaattata
     3901 ccgtcttgta attctttgat aacccttaaa tacaggtcag gatcttcctg cttgtgtgga
     3961 gggagtgagc ggtccccttt tggatcggtc atggcaccat cgcataccag atcattattc
     4021 tgtacgtatt ctaaatattt taaaaagcgt tgataatagg ttgtattata cgtttgatca
     4081 attgcatacg ttgtcattga cagagcattg agtgcgtcca ttccaacaca gcgctggaaa
     4141 caacaccctg tttgtgaacc taacagcctc cccatcttac ttttttttac taaatcatca
     4201 atactttgat gaatatgggt aaaccggttg ataatattcc cggtgatgtg ggattgtgct
     4261 gtcataatct ctttatattc atccttttga gcaagagcat aggtcattgc tactgcattc
     4321 agtgaaggct tgatgatggg atgatcaaca gggttttcta ttctttgtcc aaacatatag
     4381 atggtccggt ttaaactacg gatactttca acatagtcag aaggtgtttt caaaggcatg
     4441 agtatctcct ccttatagta tagttaatgt acattcatac atatgcactc aatactaaaa
     4501 atgtcaataa aatttatgaa tttgacagta tatatcatac acaaagttaa ataattgtat
     4561 acaacgataa gtcaattcta aagaggaaag ggcaggatgt ccaatatact ttttactttc
     4621 gccgctaaaa gtccacaact catttgtcca ttgcaaaaac tttgcatttg aaatatcaga
     4681 tattgaggag tattttttat gtttgtttat aaatcatgaa aatacaataa aagtaatatg
     4741 agattgtgta aagagtaaaa aatgagaata gattaaatca cggatgcatc gtaagccatg
     4801 acagtatata ctgtaatatc aggtctacat gctttgttga tgttaaggta tgatccccgt
     4861 caggtatgat gtgcatggtg tggggtacac acaggttttt ttgtatatca tacgcatggg
     4921 taacgggcac cacagtatca ttattgccat ggatgataca taccgggaca tcaatacgag
     4981 gcagtaagtg tatgagttgt atgctgttca actgattgag ggatgatgct gcaatggtat
     5041 attcacctac agcaacgcta tcaccattga tgcccagcgg agcaagcgtt gttactaaat
     5101 gctgcaaatg gacgggtgtt gcaatgatgc tgacactttt tattgaaaca gtatacggca
     5161 tcatggcaca gtagtacaat gctattacac ctcccatact ggaacctaca atatgaagat
     5221 ttgcattata atgattggct gcatattcca gtacggcatt gagctcatga gcacaggcag
     5281 ggagctctat ggtaagcggt gtacctgatg cataatcccg tgtatagcaa tagctaaagc
     5341 ttatgacagt atatccatgt tgacataaca cgggggcgat ctgatttatt ttataggcat
     5401 ctttagaaga aaaaagtcca tggctataga caacagtgcc ttttgaatta tgagcactat
     5461 agacaatagc gtcgacaggt tgtttatacg gaatggtaat ggtgcgatag ttcatggtaa
     5521 tggttattgt ttttgtaatg aaatcaccgg ttcaaaataa ccttctgatg ttactccatc
     5581 aattactatg gacagtggtt taagttcaag cattttttta gtatcctttt gtactagtgc
     5641 agcatgaaat acaaaatacg acgatttaac attgtaaaat ccaccagttc catacaccgc
     5701 attgatgtta tctacatttt tcatctggct tatgttaata cgaagtgttt gggggttatc
     5761 cagtgtatat actcccttaa atactacagc attgttctgt aaatagagta tgcaaacagc
     5821 ctgataattt ttagcaaacc tgaactcata gccatagttg cctctatata aaagtttcca
     5881 tcgcccatgc aaatcggtaa atttaagagc aacactattg ccagcaatca tcataaagac
     5941 tgctgtaaca atgatataat atctatagat tcttttcatg taactatcgc ttaacaataa
     6001 ctatcgcccc ataaaaggtt ggtacacatt atgattcagt ga
//
LOCUS       DAOSOX010000144         6039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13237_length_6039_cov_8.917112, whole genome shotgun sequence.
ACCESSION   DAOSOX010000144 DAOSOX010000000
VERSION     DAOSOX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13237_length_6039_cov_8.917112"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLX22_09530"
     CDS             <1..582
                     /locus_tag="PLX22_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT20178.1"
                     /translation="VDHYNKIGSDPNDVITEIEQGIVYKRNPQLFFQANRFLRGKMLS
                     IVNELTDNATHFLDVGCGVGFFSLYMSKRMEGTGIDSNTLSITFAKMNSTINTIPCNF
                     YATTIENYHPYTNCADCVIIDPPRQGISRKGRKTIIAMDPATIVYISCNPVTFARDVR
                     SFIDSNYRLQKLFMIDMFPCTHHTEVIGLMKKS"
     gene            complement(625..1194)
                     /locus_tag="PLX22_09535"
     CDS             complement(625..1194)
                     /locus_tag="PLX22_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOT20179.1"
                     /translation="MVSGQQNILKVTLSISICMHSAVLFFTFPAIQSTTPKEELTVTI
                     HGQTVLPYVEKAGDTTRLKKSTTPNSPAECHEQSITAAQNYTISGQDSEEVMMQYTDM
                     IRSRIQQALIYPSTARKDGIEGKAYVKFVIDSAGNVLSVAMVKSSGCALLDDESVKAI
                     YRASPFPRIPENLNKKSLSFVQGLTFTLK"
     gene            complement(1197..1592)
                     /locus_tag="PLX22_09540"
     CDS             complement(1197..1592)
                     /locus_tag="PLX22_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT20180.1"
                     /translation="MDIERRKKVSVELNIAPLIDVVFQLLLFFALTTSYVTNPGIEVA
                     LPKAASAVPVQKEHIELYITKDNAVYCNNEKVTMGSLRKRLENMPSLSKTVILKADKS
                     VELGMVVQVIDVVKQTNIKDLVIATTQEE"
     gene            complement(1595..2185)
                     /locus_tag="PLX22_09545"
     CDS             complement(1595..2185)
                     /locus_tag="PLX22_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT20181.1"
                     /translation="MIDIFLKGGPVMWPILFCSILSLAIFFERLYMLMKSRRYVWGAI
                     ARYKVEPLTDEGDSASCNGIVKDLLSYADNYSSNTLHDIEHRMYRFATVFIQGLEKRL
                     NWLAIIGNVAPLLGLLGTVTGMIRVFMAIQNMQGQVNPSALAGGIWEALITTAFGLIV
                     AIPTLVAYHYFEDRIDEMVNAIKDIIHVILENKHEC"
     assembly_gap    2277..2376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2546..2785
                     /locus_tag="PLX22_09550"
     CDS             2546..2785
                     /locus_tag="PLX22_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT20182.1"
                     /translation="MAGIKEKHVIQISSRRGRKKSPYLPPDYKTLQEVIDAIERDYIL
                     HTLELTNWNLQKTSRVLDIARNTLKAKMKKYNIDM"
     gene            complement(2792..3805)
                     /locus_tag="PLX22_09555"
     CDS             complement(2792..3805)
                     /locus_tag="PLX22_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20183.1"
                     /translation="MPDDKQYDSLSDQTQLINRALQYTNGNLEKAQQMVAGIFQDVAV
                     IKGKFKASKVGAFYVFLNIESNYIININAIVTTYTDIYNKIRIFDRWKQFYAMFGDIA
                     AELGASGGESSKFANHLADAIEGYNIYEPIKTGNMQLVSELFEEIIGKYFSMNADCQI
                     EIDRTTSLELENEDIPMELMDRQKADEAILTPDEMKMREIESQVEYIIPGSVIVSPVK
                     GKYINDIKQGEKISVMLTGKDPVSDRIARMFNAITSDGEYLPVNARIKEKFPLSSGGY
                     AIYALVAKNVMVKIIEEENVKIDIEKVEQKKEESNENMLFIYVALLLGLLFISALIVF
                     ALI"
     gene            complement(3806..4276)
                     /locus_tag="PLX22_09560"
     CDS             complement(3806..4276)
                     /locus_tag="PLX22_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20184.1"
                     /translation="MKQIIKKKDSGEDDSLFGLLASYKNLYSYITVIESPEQDIIAII
                     IAKNPSSFDELVKSKYQQFKPMRRIKIGVTRLFIKYITPEFIIKYFGPLLGIKVPENK
                     VHIINNLESGYFVLEIPGNYKEQVIQCIKRIVNLTPQKKYEIIEKSLADEEVII"
     gene            complement(4400..4954)
                     /locus_tag="PLX22_09565"
     CDS             complement(4400..4954)
                     /locus_tag="PLX22_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HOT20185.1"
                     /translation="MIVEIKKLAQGISVTRKSTDLFTRYVGRLLFQKIIEKLQHIAPH
                     EVVIIDFDGIRAVDASFVDECLVPLVKLSQTNETPFYGKLINISDNVEYIVGQVISME
                     KEHERYILMTDRLCKNGCHALGRLSEIEKDIIEYFVINRQATSDDIASFLHLSRDEAN
                     TVCSNLHKARAIRKIDDSYVFYCV"
     gene            complement(4959..5840)
                     /locus_tag="PLX22_09570"
     CDS             complement(4959..5840)
                     /locus_tag="PLX22_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20186.1"
                     /translation="MSTVSLPDQFKDPFSFETILSLHQTILSTKTAKLDMTNITFIEP
                     YSLLCLLLMGRNYLRLTGERLMLHRVPPHIMQYATRMDFFKYNIFTVDESLHGNAGLK
                     RSASSSRLLEIIEIPGKEKESVKVIAKVIATFRKRAIVILKNWFTLTTIDHLVTVISE
                     ICQNIFEHSLDSGFVVIQSYTYMNERIFRIAIVDSGIGVPGSFEHSPMVLSLSNAKLL
                     EAVLTTPLSSKRRYGYGLCQVNAIMEQLKGSIFLRSENAYISVLHHKRAAKGSYIFLK
                     DDCPTFPGTQFSITLAG"
     gene            complement(5851..>6039)
                     /locus_tag="PLX22_09575"
     CDS             complement(5851..>6039)
                     /locus_tag="PLX22_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20187.1"
                     /translation="VLPMNSNYRPCYKNMLKKSNSTLLLICYAGNNKIEEYRLKIFTK
                     MSMSIAGDVIILNAISKI"
BASE COUNT         1818 a         1185 c          956 g         1980 t
ORIGIN      
        1 gttgatcact ataataaaat aggctctgac cccaatgatg tcatcaccga gattgaacag
       61 ggtatcgttt ataagcgaaa tccacagctt ttcttccagg caaataggtt tcttcgcggg
      121 aaaatgcttt ccattgtcaa tgagcttacc gacaatgcta cacacttttt agatgttgga
      181 tgcggcgtgg gatttttcag cctttatatg tcaaaacgaa tggaaggcac cggcatagat
      241 tcaaatacat tgagcattac atttgcaaaa atgaattcaa caatcaacac tattccatgc
      301 aatttttatg caacaactat cgagaactac catccgtata caaactgtgc ggattgcgtt
      361 attatcgacc ctccacgtca gggtataagc aggaagggac gtaaaaccat catagctatg
      421 gacccggcaa cgatagtcta tatttcatgc aatcctgtta cctttgctcg cgacgtcaga
      481 agctttattg atagcaacta tcgcctgcaa aagcttttca tgattgatat gtttccgtgt
      541 acccaccata ccgaggtcat tggattgatg aaaaaaagct aatcacagga tattaccatg
      601 cattggcaaa caattacaaa accattattt aagggtaaac gttaaaccct gaacaaacga
      661 tagtgatttt ttattgaggt tttcaggaat acgtggaaaa ggtgatgccc tgtatatagc
      721 cttcactgat tcatcatcca acaaagcaca tcccgatgat ttcaccatcg caaccgacag
      781 tacattccct gcactatcta ttacaaattt cacataggct ttaccttcta tgccgtcttt
      841 ccgtgcagtc gaaggatata taagtgcctg ctgaatacga cttcgtatca tgtcggtata
      901 ctgcatcata acttcttcac tatcctgacc tgaaattgtg tagttttgtg ctgctgtgat
      961 tgattgttca tgacattctg cgggactatt gggcgtggta ctttttttta accgtgttgt
     1021 atccccggct ttttccacat agggcaatac agtctgacca tggatggtta ctgttaactc
     1081 ttcctttggg gttgttgatt gaattgctgg aaatgtaaaa aaaagcacag cactatgcat
     1141 gcagattgaa atgcttaacg tcacttttaa tatattctgt tgcccactca ccattgtcat
     1201 tcctcttgcg ttgttgctat cacaagatct tttatattag tttgcttaac cacatcaatg
     1261 acctgcacca ccatccccag ctctacactc ttatcggctt tcaggattac tgttttagat
     1321 aacgagggca tattttccag cctttttctt aaactaccca tagttacctt ttcattatta
     1381 caatatacag cattatcttt tgtaatatac agttcaatat gttctttttg tacgggaacg
     1441 gctgatgcag cctttggtaa cgctacttct ataccgggat tggttacata tgatgttgta
     1501 agcgcaaaaa ataaaagaag ctgaaagacc acatcaataa gcggcgctat gttaagttca
     1561 actgaaactt tttttctacg ttcaatatcc acagctaaca ctcgtgttta ttttccagta
     1621 ttacatggat tatatctttt attgcattta ccatctcatc tattcgatct tcaaaataat
     1681 gatatgcaac aagtgttggt atggcaacaa taagtccaaa tgctgtggtg ataagtgcct
     1741 cccatatacc gccagcaagc gccgaaggat ttacctgccc ctgcatattt tgtatagcca
     1801 taaatacccg tatcatccct gtcactgttc ccaataatcc cagcagtggt gcaacattgc
     1861 ctatgattgc cagccagttg aggcgctttt ctaaaccctg gataaaaact gttgcaaagc
     1921 gatacatcct gtgttcaata tcatgcaatg tgttgctgct gtaattatct gcatatgaca
     1981 gtagatcttt tacaatccca ttgcatgatg cactgtcacc ttcatctgtt aacggttcta
     2041 ccttataccg ggcaattgcc ccccacacat atctgcgtga tttcatgagc atatataacc
     2101 gctcaaaaaa gattgcaagc gatagtattg aacaaaaaag tattggccac attaccggcc
     2161 cgccttttaa aaaaatatca atcataggtt cacaccgtta caaattattt ataattgcta
     2221 caatatgaat atcatactga aaataatgca acagttttat gtttttttca cagtttnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat ataaatattt ttttaataat
     2401 ataaaaaatg tattgaatat ttttatggga atgatttaag ctgaaccagt catactatag
     2461 tttaatatat gacatttttt agatatcatt ttacatattt tgtatattag taatttaaat
     2521 ttttttgatg agcaaggagt tctctatggc tggcataaaa gaaaagcacg tgatacaaat
     2581 cagctcccgc aggggcagaa aaaaatctcc ctatctacct cccgattaca aaacactgca
     2641 ggaggttatt gatgcaatag aacgtgatta tattctgcat actttagaat taactaattg
     2701 gaatcttcag aagacaagcc gtgtattaga tatagcccga aatactttaa aggctaaaat
     2761 gaagaaatac aatattgata tgtaataatt atcatattaa ggcaaatact atgagggcag
     2821 atatgaataa taaaccaaga aggagagcta cgtaaataaa taacatattt tcattgcttt
     2881 cttctttttt ttgttctacc ttttcgatat caatttttac gttttcttct tcaattattt
     2941 taaccattac attttttgct acaagtgcat agatagcata accgccactg gaaagaggaa
     3001 atttttcttt tatgcgggca ttgacaggca gatattcacc atcacttgtt atagcattaa
     3061 acatgcgtgc tattctatct gatacgggat cctttcctgt aagcattaca gaaatttttt
     3121 caccctgctt aatgtcattt atatattttc ccttaacagg tgatacaatg accgaacccg
     3181 gaataatata ttcaacctgt gattctatct cgcgcatttt catttcatca ggggttaata
     3241 ttgcctcatc agctttctgt ctatccatta actccatggg aatatcttca ttttcaagtt
     3301 caagtgatgt cgtcctgtct atctctattt gacagtcagc attcattgaa aaatatttgc
     3361 caattatctc ctcaaaaagc tctgacacta actgcatatt gcctgtttta attggttcat
     3421 agatattata tccttcaatt gcatcagcta aatgattggc aaactttgat gactcgcctc
     3481 ctgaagcacc taattcggca gcaatatcac caaacatggc ataaaattgc ttccacctat
     3541 caaagatacg tattttatta tatatatcag tataggtagt aactatcgca ttgatattaa
     3601 taatataatt gctttcaata ttgagaaaca catagaatgc accaacttta ctggctttaa
     3661 attttccttt tattaccgcc acatcctgaa aaatccctgc aaccatctgc tgggcctttt
     3721 ccagattccc attggtatat tgtaaagctc tattaataag ctgggtctga tcactcaaag
     3781 aatcatattg cttatcgtcc ggcatctata ttattacctc ttcatcagct aaactttttt
     3841 caattatttc atattttttt tgtggtgtca ggttaacaat gcgttttata cattgtatta
     3901 cctgttcttt ataattacca ggtatctcca gtacaaaata accactttca agattattaa
     3961 taatgtgtac tttattttct ggaactttta taccaagcaa tggcccaaaa tatttaataa
     4021 taaattcggg cgttatgtat ttaataaata accttgtaac acctattttt attcttctca
     4081 ttggtttaaa ttgctggtat ttacttttta ctaattcatc aaatgacgat ggatttttag
     4141 ctatgataat agctatgata tcctgttctg gcgattcaat tacagtgata taactgtaaa
     4201 gatttttata tgaagccagc aatccaaaca gcgaatcatc ttctcctgaa tctttttttt
     4261 taattatctg tttcatagag tagcattcta ttatagtatc aaaaaataaa tgccgttatt
     4321 gcaagagcta tcgcctgcaa tggagcgtca ctttttaact caatactata tataaaggca
     4381 ttattaatca agtattttac taaacgcagt aaaaaacata actgtcatct atttttctga
     4441 tagctcttgc tttatgaaga tttgaacaca cagtgtttgc ctcatctctg gacaaatgca
     4501 aaaaagaagc aatatcatca gatgtagcct gtctgttgat aacaaaatat tcaataatgt
     4561 ctttttcaat ttcagaaagc ctccccagag catggcatcc gtttttacat agtctatcgg
     4621 tcataagaat atatcgttca tgttctttct ccatactgat tacctgaccc acaatgtatt
     4681 caacattgtc acttatattg ataagtttac cataaaaagg agtttcattt gtttgcgaca
     4741 gttttacaag agggacaaga cattcatcaa caaaggaagc atcaactgct cgtatgccgt
     4801 caaaatctat gatgacaacc tcgtggggag caatatgttg caatttttct ataatctttt
     4861 gaaacagcaa tcttccaaca tagcgtgtaa atagatccgt acttttacgg gttacgctaa
     4921 tgccttgcgc taattttttt atttctacaa tcatgcattt atccagcaag tgttattgaa
     4981 aactgtgttc ccgggaatgt tgggcagtca tcctttaaga aaatataact tccctttgcc
     5041 gcacgcttat ggtgcagcac actgatatat gcattttcag atcgtaaaaa tattgagcct
     5101 ttaagctgtt ccattatcgc attaacctgg catagtccat aaccatagcg ccgtttactt
     5161 gacaacggtg tggtcaatac cgcttcaagc aattttgcat tggacaaact caaaaccatg
     5221 ggtgagtgct caaacgatcc gggaacgcca atccctgaat caacaatagc aatcctgaaa
     5281 atacgttcat tcatataggt ataactctgg atgacaacaa accctgaatc aagactatgt
     5341 tcaaaaatat tctgacatat ttcggatata acagtaacaa gatggtctat cgttgtcagg
     5401 gtaaaccagt tttttaaaat gacgattgcc cgttttcgga atgtagctat aacctttgca
     5461 ataaccttta cgctttcttt ttccttcccg gggatttcaa taatttccaa taaacggctg
     5521 gatgaagcac ttcgttttaa tcctgcattc ccatgcaacg attcatctac agtaaatata
     5581 ttatacttaa aaaaatccat ccttgtagca tactgcataa tatgaggcgg aacacggtgc
     5641 agcataagcc tttcaccggt aagccgtaaa taattacgcc ccatgagcag caaacacagc
     5701 aatgaatatg gctctatgaa ggtaatattt gtcatatcaa gctttgcagt tttagtggat
     5761 agtatagttt gatgcaaaga aagtatcgtt tcaaagctga aagggtcctt gaattgatcg
     5821 ggtaaggata cagtactcac tatcaatcct ttatatttta gaaatagcat tcaggatgat
     5881 tacatcccct gctatactca tcgacatttt tgtaaaaatt ttcaaacggt attcctctat
     5941 tttattgtta cctgcgtagc atattaacag taatgtacta ttcgattttt tcaacatatt
     6001 tttatagcat gggcgatagt tactgttcat gggcaatac
//
LOCUS       DAOSOX010000145         6032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13255_length_6032_cov_8.460264, whole genome shotgun sequence.
ACCESSION   DAOSOX010000145 DAOSOX010000000
VERSION     DAOSOX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13255_length_6032_cov_8.460264"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLX22_09580"
     CDS             complement(<1..690)
                     /locus_tag="PLX22_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20188.1"
                     /translation="MKRYFFWTSLCIIVALVACGGAQKQQKPSKTACEMCGEADGFAA
                     IFDNDTALARDRAIDDAMNKLVKMKLGTSIEGRTVVEDYALVESIVEAKSTGMVRNWK
                     VIKEGAQEGAYTVRIYGEVYPQAVNDTIEATLKNYGRPKFMILINETFEGQKHEPGFT
                     VTELTMMEIMQNAGFEFVDAATVQQLIKKDRAKMMRAMQGQVGGAVQELLLDDVGAEC
                     IIVGDVTTTDQS"
     gene            944..3061
                     /locus_tag="PLX22_09585"
     CDS             944..3061
                     /locus_tag="PLX22_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOT20189.1"
                     /translation="MSLSSLYNLPVDQITRESYFNVGGMHGFMRWGVYIFLIIAAIFI
                     LYSLIQKALIWRRGKPELRTDFPEKRIIALIKYVFLQKKVLREKYAGIMHACLFYGFI
                     GLFIVTFIILIQEDVTELFFHFKFLVGDAYLLWSFAGDIFGVAVIVGLVMAFWRRYKV
                     KPSRLDTKPIDTFALILIAFIILTGFLNESLRIAITGFPKFEVWSPVGYSLAHLFGWL
                     SIPTLEITHYINWWLHMIGAFSFIGLIATDKLGHILTTMLNVYFQNLDNESPKTKFAM
                     PVISPKEFETAETFGVGMVEHFTWKQLMDGDACTRCGRCQDNCPAYLTEKPLSPKKII
                     NDVKDAMDDRIPKLMTADDPSTVESKALIGEYVLDDEIWSCTNCAACMENCPVQIEHV
                     PKIVDMRRYQVLMEGKMATELQTSFGNMENNSNPYGFAFAARADWVTEDLGVKTLAED
                     PNVDFVYFVGCAASYDKRNQKVALALCKILQAAGYKVGILGPEEACCGDSAMRAGNEY
                     LFHALATQNLETFKNYGIKKIITTCPHGYNTIKKEYPKFAKVGVDSSGNPLEANYEVY
                     HHTEVIMNLIKSGKIRLTKPLNETITYHDSCFLGRYNTIYDQPRNILKAIPGTNFVEM
                     SRNHERSFCCGAGGARMWIEEHLGTRINQFRTKDAQATGATRIGTACPFCLTMLSDGA
                     NELDIQNLQTFDLAEYVWEAMEK"
     gene            complement(3138..3662)
                     /locus_tag="PLX22_09590"
     CDS             complement(3138..3662)
                     /locus_tag="PLX22_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT20190.1"
                     /translation="MPLYEINDRKPVIGEGTWIAPSAQIIGDVRIGSRCYIGFGAIIR
                     GDYGTIIIGDESAIEELCMIHARPMQLVTIGRRVTVGHMVMIHGATIMDNAVIGMHAT
                     VSDNAEVGEWAIVAEHALVVNKQRIPAYKIYGGVPAKEIGDVQQKHKDIWDAGKQVYV
                     DLTYQYPQSFHKID"
     gene            complement(3678..4619)
                     /locus_tag="PLX22_09595"
     CDS             complement(3678..4619)
                     /locus_tag="PLX22_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT20191.1"
                     /translation="MDNTITLYSKVKIILKRYFEALNMEQRVVVPQYDADYKPKWITI
                     ILLFIATFITTSIAGSNAGGSILEIIINGLPFSLSLLAILFAHEMGHYITARYFGVIA
                     TPPYFIPFPSLIGTMGAIIRIKSPIPDKRVLLYIGAMGPIAGFIISCILVIVGLMQSS
                     VQPLPKPSGDSFVPIFGDSLLFVFLVKMILGNIPQGYDVYFSPLAWAGWIGFLVTSLN
                     LMPIGQLDGSHVLYALFGEKQRYAGWLFFGIICILTFLWVGWVVWIILTVSLLMIGHP
                     YIPDGKPLTAAEKIMGYLCIIIFIFTFIPVPVVISGQ"
     assembly_gap    4635..4734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4735..>6032)
                     /locus_tag="PLX22_09600"
     CDS             complement(<4735..>6032)
                     /locus_tag="PLX22_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT20192.1"
                     /translation="VSVGNEADISIIDVLEYLANDEQTKAIALYIEGIRDIKRFLNVA
                     ITITSKKPIIAQYVGGSQAGARAGSSHTGAMAGPDYLYEGLFKQAGIIRVHSIEELYF
                     HGYMLATQPGLKGNRVGIITNSGGPATSMANSCEKEGFIVPTFSEGLQSNIKPLIPPH
                     AQAGNPVDITFNLDLDVLTIKIPELAIQSGEVDALCIHGAYRKGFVEAVYPHIREILN
                     IQKPEDIINMFPSDLHTPGTIPKKYGIPLVMSSFYDYQDDFTKAMQDHDVLVFDAPEK
                     AAKALAALLRYKEIINRLPYEPITLPETNSEAKAIIQGAKKNNQKALDEYMAKRLLSL
                     YGIPIPDERLIENEKQLDGIVTSMSYPIVMKACHPNIMHKTEKGLVMLPIKDSIEAKQ
                     AFQTIQSRAGYAIPVLAYPMVEGKREFMVGAVNNKGFAQSV"
BASE COUNT         1825 a         1213 c         1062 g         1832 t
ORIGIN      
        1 tgactggtct gttgttgtaa catcacctac tatgatacat tcagctccaa catcatcaag
       61 caaaagctcc tgtacagccc caccaacctg tccctgcatc gcccgcatca tctttgctct
      121 gtcttttttt attaactgct gaacagttgc tgcatccaca aattcaaacc cggcattttg
      181 cataatctcc atcatggtaa gctctgtaac agtgaagcct ggttcatgct tttgaccttc
      241 aaaggtttca ttgatgagta tcatgaactt aggacgccca taatttttca atgttgcctc
      301 tatagtatca ttaacagcct gtggatatac ctcaccataa attctgactg tatatgcacc
      361 ttcctgtgca ccttctttaa taactttcca gtttcttacc atacctgttg attttgcctc
      421 aacaatggat tctactagcg cataatcttc aacgactgta cgtccttcta tggaagtacc
      481 cagcttcatc ttcaccaatt tgttcatagc atcatcaatg gctctatcac gtgcaagtgc
      541 tgtatcatta tcaaatatgg cagcaaaccc atcagcttca ccacacatct cacatgctgt
      601 ttttgatggt ttttgttgct tctgagcacc tccgcatgcc actaatgcaa caatgataca
      661 taaacttgtc cagaaaaaat atcgtttcat aattggacct cataattatt gaatatttat
      721 tgtatatgaa tgatactata gtaccatatt aaagttacgt caaaccattt ttataaatta
      781 tcaattacca cattctaaaa ttaccaacat gttcataggt tactattcaa tcataatatt
      841 ctcactattc tatttaaaat agtgttgaca aggacttcca gtattaaagt attgttaact
      901 aaagttagat gcaattattt tttatggata aggagggttg tacatgagcc tttcaagttt
      961 atataatctg cctgtcgacc agataaccag agaatcgtat tttaatgttg gcggcatgca
     1021 tggtttcatg cgatggggtg tatatatctt tttaataatc gctgccattt ttattcttta
     1081 cagcctgatc caaaaggcac taatctggag gcgtggcaaa ccagaactca gaacagattt
     1141 cccggaaaaa agaattattg cgcttattaa atatgtattt ttacaaaaaa aagttttacg
     1201 cgaaaaatat gcagggatca tgcacgcctg cctgttctat ggttttatag gactattcat
     1261 tgtaacattt ataatcctga ttcaagagga cgttactgaa ctattcttcc attttaaatt
     1321 tttggtaggg gatgcatacc tgttatggtc atttgcaggc gatatttttg gcgttgcggt
     1381 tattgtaggg cttgttatgg cattctggag gcgttataaa gtaaagccaa gcaggctgga
     1441 tacaaaacca attgatacct ttgcactgat attaatagct tttattatcc ttaccggatt
     1501 tttaaacgaa tcgctcagaa tagccattac gggattccca aaatttgagg tatggtcacc
     1561 ggttggttat tcattagcac acctttttgg ttggttaagt attccaaccc ttgagataac
     1621 ccactacatc aactggtggt tgcatatgat tggtgcattc agcttcatag gactcattgc
     1681 taccgataaa ttagggcaca tccttaccac catgctcaat gtctatttcc agaatcttga
     1741 caatgaaagt ccaaaaacaa aatttgcaat gcctgtaata agcccaaaag aatttgaaac
     1801 agccgaaaca tttggtgtag gtatggttga acactttaca tggaaacagc ttatggatgg
     1861 tgatgcatgt acacgatgtg gacgctgtca ggataattgt cctgcatacc tgactgaaaa
     1921 accactatct cctaaaaaaa ttatcaatga tgtgaaagat gcaatggatg atcgcatccc
     1981 gaaacttatg accgcagatg atccatcaac tgttgagtca aaagctttga ttggtgaata
     2041 tgttttagat gatgagatat ggtcatgtac caactgtgca gcatgtatgg aaaattgccc
     2101 cgttcagatt gagcatgtac caaaaattgt tgatatgcgc cgttatcagg tccttatgga
     2161 agggaagatg gcaacagagc tgcaaacatc atttggtaac atggaaaata attctaatcc
     2221 ctatggattt gcctttgctg ctcgtgctga ttgggttact gaagatttag gagttaaaac
     2281 attagccgaa gaccccaatg ttgattttgt atattttgtt ggctgtgcag catcatatga
     2341 taaacgcaat cagaaagtag ctctcgctct atgtaaaatt ttacaggcag ccggatataa
     2401 agtaggtata ttaggtcctg aagaagcatg ttgtggcgat tctgcaatgc gcgcaggcaa
     2461 tgaatatcta ttccatgcac tggcaacaca aaaccttgaa acattcaaaa attatggtat
     2521 aaaaaagatt attaccacct gccctcatgg atataatacc attaaaaaag aatatccaaa
     2581 atttgcaaaa gttggcgttg attccagtgg taatcctctt gaagcaaact atgaggttta
     2641 tcatcacact gaagttatta tgaacctcat taaatcaggt aaaataagat taacaaaacc
     2701 actgaacgaa accatcacct atcatgattc atgtttcctt ggacggtata ataccatcta
     2761 tgatcagcca agaaacatcc tcaaggcaat acccggaact aactttgtcg aaatgagcag
     2821 aaatcatgag cgtagttttt gttgcggcgc tggtggtgca cgcatgtgga ttgaagagca
     2881 tctgggaaca cgaatcaatc aattcagaac aaaagatgca caggctacgg gagcaaccag
     2941 gataggtact gcatgcccat tctgtttaac catgctttca gatggtgcaa acgaacttga
     3001 tattcagaat ttgcagacat ttgatcttgc agaatatgtc tgggaagcaa tggaaaaata
     3061 atgtaaaaaa aatagtaata cattacaatt atgaaaaagc atcgccaatt tgacgatgct
     3121 tttttatttt aatcaattta atctatttta tgaaaagatt gtggatattg gtaggtcaaa
     3181 tcgacataca cctgtttccc ggcatcccat atatctttat gtttttgctg tacatcacca
     3241 atctctttgg ctgggacacc accatatatt ttataagcag gtattctttg tttattgaca
     3301 accagcgcat gttcagcaac tatcgcccat tctcctacct ctgcattatc acttacggtt
     3361 gcatgcatgc ctattactgc attatccatg atagtagcac catgaatcat aaccatgtgg
     3421 ccaacggtta cgcgtcttcc tattgtaacc aattgcatgg ggcgtgcatg aatcatacac
     3481 aattcttcaa tagcagattc atcgcctata ataatagtac cgtaatctcc acggataatt
     3541 gctccaaatc caatatagca tcggctacca atcctgacat caccaattat ctgcgcagat
     3601 ggagctatcc atgttccttc accaataaca ggcttccgat cattaatctc atagagtggc
     3661 atgctaccct cccttattta ttgtcctgat ataactaccg gtacaggaat aaaagtaaat
     3721 ataaaaatta ttatgcataa ataacccatt attttctctg ctgctgttaa tggcttccca
     3781 tcagggatat agggatgacc tatcataagc aaacttacag ttaaaattat ccagactacc
     3841 catcccaccc ataaaaaagt aagaatacat ataataccaa agaatagcca gccagcatag
     3901 cgttgctttt ccccaaatag tgcgtataaa acatgactgc cgtccagttg tccaatgggc
     3961 ataagattta agcttgttac cagaaagcct atccagccag cccatgccag cggagaaaaa
     4021 tatacatcat aaccctgtgg gatattgccc agtatcattt ttactaaaaa aacaaacaga
     4081 agtgaatcac cgaatatagg tacgaaacta tcaccagatg gtttcggaag aggttgtaca
     4141 cttgattgca taagtcctac aatcaccagt atacagctta taataaaccc tgctatgggt
     4201 cccatggctc caatgtataa taaaacacgt ttatcaggaa tgggcgactt tatgcgtatt
     4261 atggcaccca tggtacctat caatgatgga aacggtataa aatagggggg tgttgcaata
     4321 actccaaaat agcgcgcagt gatataatgc cccatttcat gggcaaaaag gatagcgagc
     4381 aatgataatg aaaatggtaa accattgatg ataatttcaa gtatggaacc accggcattt
     4441 gaaccagcaa ttgatgttgt tataaatgtc gctatgaaga gcaaaattat ggtaatccat
     4501 tttggtttat aatcagcatc atattgtggt acaactactc gttgctccat gttaagcgct
     4561 tcaaaatagc gcttgagtat tatctttact tttgaataca atgttatggt attgtccatg
     4621 gtattattct attannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnactgat
     4741 tgagcaaacc ctttattatt aaccgcacca accataaatt cccgtttccc ttcaaccatg
     4801 gggtatgcca gcaccgggat agcataacca gcacgacttt gaatggtttg aaatgcctgc
     4861 tttgcttcaa tactgtcctt tataggtaac ataacaagtc ccttttctgt tttatgcata
     4921 atattggggt gacatgcttt catcactata ggatagctca tggatgtaac aataccatct
     4981 aattgctttt cattttcaat cagcctttca tcagggattg gaataccata caaggacaac
     5041 aatcgtttag ccatatattc atccaaggct ttctgattat tcttcttagc accctggata
     5101 attgcttttg cctcagagtt ggtttctggt aatgtgatag gttcatacgg taaacggttt
     5161 ataatttcct tatagcgcaa taatgcagct aatgccttcg cagccttttc aggtgcgtca
     5221 aacacaagga catcatgatc ttgcatggct tttgtaaaat catcctgata gtcataaaac
     5281 gatgacataa ccagagggat tccatatttt ttggggatag ttcctggtgt atgcagatca
     5341 ctggggaaca tattgataat atcttcaggt ttctggatat ttaaaatctc tcgtatatga
     5401 gggtaaactg cttcaacaaa tccttttcta taggcaccat gtatgcaaag agcatcaacc
     5461 tcaccacttt gtattgccag ttccgggatt ttaatggtta atacatctaa atccagattg
     5521 aaggttatat caacagggtt acctgcctgt gcatgaggtg gtataagagg tttaatgtta
     5581 ctctgtaatc cctcactaaa tgttggaaca ataaatcctt ctttctcaca gctatttgcc
     5641 attgatgttg caggacctcc tgaattggta ataatcccga cgcgatttcc ttttaaccct
     5701 ggctgcgttg ccagcatata cccatgaaaa taaagttctt caatagaatg aaccctgata
     5761 ataccagcct gtttaaataa tccttcataa agataatccg gtccggccat agcaccggta
     5821 tgacttgaac cagcacgagc tccagcctga gaaccaccaa catattgagc tataatcggc
     5881 ttttttgatg ttattgttat agccacattc agaaaacgtt ttatatcacg tattccttca
     5941 atatacaatg caatagcttt tgtctgttca tcattagcaa ggtattctaa aacatcaata
     6001 atggatatat ctgcttcatt gccaacactt ac
//
LOCUS       DAOSOX010000146         6012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13324_length_6012_cov_9.200772, whole genome shotgun sequence.
ACCESSION   DAOSOX010000146 DAOSOX010000000
VERSION     DAOSOX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13324_length_6012_cov_9.200772"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="PLX22_09605"
     CDS             complement(<1..1418)
                     /locus_tag="PLX22_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT20193.1"
                     /translation="MKQRFKKHFERKPIEELMEEAGHEHEYSLKRVLSPFHLIMLGVG
                     VIVGAGIFVLTGHVAAAYSGPAIVLSFVIAAIACGFAGLCYAEFASMISVSGSAYTYA
                     YATLGKLFAWIIGWDLILEYLFGASSVAVGWSGYVCSFLKDFGIVIPASFASAPFGYD
                     IHTGWYLTGAIVNVPAVLIIVIMTILLILGIKESATFNNIIVVIKVSVIVLFIGFGIF
                     FINFENMVPFIPPNTGHFGEYGFSGILRGAGVIFFAYIGFDVVSTAAQEVKNPKRDMP
                     IGILGSLAIVTVLYILVAFVITGLVSYTKLNVPDPIAVAIDAAGEHLFWLRPIIKIGA
                     ITGLSSVVLLLLYGQSRIFFTMARDGMLPSVFARVHQKYRTPYITTIVVGVAAVIFSG
                     LFPIGILGEMVSIGTLLAFVIVCIGVLVLRYTRPKLHRPFKTPFVPFVPIMGALVSFI
                     QMVALPKDTWLRLLIWMFVGFI"
     gene            complement(1643..1728)
                     /locus_tag="PLX22_09610"
     tRNA            complement(1643..1728)
                     /locus_tag="PLX22_09610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1692..1694),aa:Leu,seq:taa)
     gene            complement(1733..1805)
                     /locus_tag="PLX22_09615"
     tRNA            complement(1733..1805)
                     /locus_tag="PLX22_09615"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1770..1772),aa:Cys,seq:gca)
     gene            2386..4791
                     /locus_tag="PLX22_09620"
     CDS             2386..4791
                     /locus_tag="PLX22_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOT20194.1"
                     /translation="MVRRIEKAAVIGSGIMGGGIAALLADTGVNTLLFDIVPFNLTDE
                     EKKDPKARYRIVQAGFDGLMKAKPAAFMDKSNASRISLCNLEDDFDKLKECDLIIEVV
                     VENLKIKQDLFQKLDKIRKPTAIVTSNTSGLPLKEMSKNCSDAFKEHFMGTHFFNPVR
                     YMHLLELIPGEKTKKEVLEFIADFGEKRLGKGIVWAKDTPNFIGNRIGVHEIMTAMKL
                     LESENITIDEADAIMGPAMGRPKTAIFKLSDMVGLDTIHHLAENSYQLLEKDERREVY
                     KVTPWFNKMVENKWLGDKTKQGFYKKEKTPDGKRLSLVLDPVKMEYREAQKPEFESVS
                     AAKKLPTAAEKIKAIVWGKDKAAQFAWKLTAYDAIYAANRIPEIADSIVEIDNAMTWG
                     YNLELGPFQAWDAIGVKESVERMEKEGMKVPENVKKMLASGATSFYKVENGKEYYYDL
                     VNGGYKEVKKSEAAISIFNLKGAGKEVKKTNSISLIDLGDGVFCFEFHSKMNAINGEI
                     VDFMLANIDYVKENGVGAVIGNQAGGMPGAFSAGGDLAYMGSLAKAKKWSEIDKFIAN
                     VHTSQLAAKYAPIPVVAAPYGMTLGGGCEVCLNADRIVAHAELYMGLVEIGAGLLPGG
                     LGMMNLWRKYLKSVPKAAKITDLAGLFLPCFMAVAQAQVTMSAFEAQKKGFLGAKDRI
                     VFNRDLLIGEAKKEVLRMVDEGYAPPKKEKFAVLGREAQGMVDAEMLNMRIGMYITPH
                     MEFIAKKIAWVMSGGDVPSGMEIDEAHWLKLEREAFVELWQTENTQKMAEHILQTGKP
                     LFI"
     gene            4828..>6012
                     /locus_tag="PLX22_09625"
     CDS             4828..>6012
                     /locus_tag="PLX22_09625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT20195.1"
                     /translation="MRDAYIVQAVRTPGCRRGKGAFAQTRPEDLLVTALQGVIERAKV
                     DKKVVDDIMVGCAFPEAEQGLNVGRIAAQMAGFPDDTCGATVNRFCASGLEAIAISTM
                     RIQAGWCDVAIGAGLESMSIVPMGGNMPRPHPYWAKQNPDVYISMGITAENVANRYKI
                     TRQMQDEFAYHSQMKAAEAQKNKLYNEIVPTKATKFVPQPDGTIKKEEFMQDFDDGVR
                     SDTTIEGLGKLKPAFAAFGSVTAGNSSQTTDGSAATLLMSEEACRKYGVKPLAKVKAY
                     VVAGCKADEMGVGPAYAIPKLLKLVGLDIKDIGIFEINEAFASQAVYCAQQLGIADPK
                     EWAVDSKTRRINVNGGAIALGHPLGCTGAKLAAQIVNMMTKNGIKYGIESMCIGGGMG
                     AAA"
BASE COUNT         1964 a         1112 c         1256 g         1680 t
ORIGIN      
        1 ataatgaatc ctacaaacat ccagatgagc aatctcaacc atgtatcttt tggcaatgct
       61 accatctgta taaatgatac cagcgctccc atgatgggaa caaacggaac aaaaggcgtt
      121 ttaaagggcc tgtggagttt aggacgagta taccgcaata ccagcactcc aatacacaca
      181 ataacaaatg caagtaatgt tccaattgat accatttcac caaggatgcc tatggggaac
      241 aatccagaaa aaatgacagc ggcaacacct actacaatgg ttgttatata cggtgtcctg
      301 tacttttggt gaactctggc aaaaacagat ggcagcatcc catcccgtgc catagtaaaa
      361 aatattcttg actgtccata gagcaacagc aacacaacag aactcaaacc agttatcgct
      421 ccaattttta taataggccg caaccagaat agatgctcgc cagcagcatc aatagcaaca
      481 gctataggat ctggaacgtt taattttgta tagcttacca gcccggtaat aacaaaggca
      541 accaggatgt ataaaacagt aactatcgcc aatgaaccaa gtatgccaat gggcatatcg
      601 cgtttgggat ttttaacctc ctgtgctgcc gttgatacaa catcaaaccc aatgtatgca
      661 aaaaatatta cccctgctcc tcgtaatata ccgctaaatc catattcgcc aaaatgtccg
      721 gtgtttggcg gtataaaggg aaccatattt tcaaaattta taaaaaatat accaaatccg
      781 atgaaaagca ctatgacact aaccttaata actacgatga tattattaaa ggtagcagat
      841 tcctttattc ccagtatcaa caaaatggtc atgataacga ttatcagaac agcaggcaca
      901 tttactattg cacctgttag gtaccatccc gtatgaatat catatccaaa tggagcactg
      961 gcaaaagaag caggaataac aataccaaaa tctttcaaga agctacacac gtaccctgac
     1021 cacccaacag ctaccgacga agctccaaaa agatattcaa gaataagatc ccagccaatg
     1081 atccatgcaa agagcttccc taacgtggca tatgcatagg tatatgcact tcctgaaact
     1141 gatatcatgg atgcaaattc agcataacag agccctgcaa aaccgcatgc tattgcagca
     1201 atgacaaatg aaagtacgat tgccggtccc gagtatgcag cagcaacatg accggtaagt
     1261 acaaatatac cggcaccaac tattacacct acaccaagca taattaaatg aaatggactg
     1321 agtactcgct tcagtgaata ctcatgttca tgaccggcct cttccataag ttcttctatt
     1381 ggcttacgtt caaaatgctt tttaaaacgc tgcttcatgc catagcacct gcatcagtaa
     1441 atattaatgt tcattgatta caacacatta ttgaaagatg attatactat acgtttttta
     1501 tcaacaaaaa ttataatttt ttatcaaaca agccttatac catacaaaag ttttacgata
     1561 attacagtta ttgataaaat ttggaaaatt ggaaaagttg aagaaatggt gtcttactgt
     1621 gtctgactat tgtaacaata attgcccaag gccggacttg aaccggcacg gacttgtaag
     1681 tccaagggat tttaagtccc ttgtgtctac ctgttccacc acttgggcaa tgaggcgccg
     1741 agcggattcg aaccgctgca taaaggtttt gcagacctct cccttagcca cttgggtacg
     1801 gcgccagact aaccatagta cattggaata atttgtatca tcaataataa cacataattt
     1861 tactgtcaac ttaaaaaaat atatttttaa tgcagcatta taaccgtatg gtacaaagga
     1921 tagtaaaatt ccggaaatat accatttacc attattgaag acatgacact atcgctgaca
     1981 tcttgtaact aatttaaatt ttttttagat ttccctatga tgtaatcttt atcagtataa
     2041 agctgaaact tttattttga tatggtttat tatgataatt ttatcaaatt atgaacatac
     2101 tttgctatgg acatttgata caatttttta aagctgtact ggaaatattt tctttattta
     2161 atagtaaaca aaatatggta aatattatat tatcataata tgacttatgt ttataattat
     2221 ttttataaca taaatttcat atactataaa atattaaatt attagtgttt ttcaataata
     2281 aaatattgac aatttatttt attattccat cattggtaca tgtttgtatt tacgcaggag
     2341 tatgcaaatt ttcatttaat tattacatct tagtaggagg ttcttatggt tagaagaatc
     2401 gaaaaggcag ccgttatagg ctccggtatc atgggtggtg gtatcgcggc tttgttggct
     2461 gacacaggcg taaacactct cctcttcgac atagtaccat ttaatttaac agatgaggag
     2521 aagaaggatc caaaggcacg gtatcgcatt gtccaggctg gttttgatgg cttgatgaag
     2581 gctaaaccag cagcatttat ggataagagc aatgcatcgc gaatcagtct atgtaatctt
     2641 gaagatgatt ttgacaaatt aaaagaatgc gatttaatca ttgaggttgt tgttgaaaat
     2701 cttaagatta aacaggattt atttcaaaaa cttgacaaga ttcgcaagcc aactgcaata
     2761 gtaacatcta atacttccgg cttaccgctc aaagagatgt caaaaaattg tagcgatgca
     2821 tttaaagaac atttcatggg cacacacttc tttaacccgg tgcgttacat gcatctgtta
     2881 gagcttatcc ctggcgaaaa aaccaaaaaa gaggttcttg aatttattgc tgattttggt
     2941 gaaaaacgtc ttggtaaagg tattgtttgg gcaaaggata caccaaactt tattggtaac
     3001 cgaattggcg tccatgaaat catgaccgct atgaagctgt tagaatcaga aaacattacc
     3061 attgatgaag ctgatgctat catgggacct gcaatgggtc gtccaaagac agcaatattt
     3121 aaactttctg atatggttgg tctcgacaca attcatcatc ttgcagaaaa ttcataccag
     3181 cttctggaaa aagatgaacg ccgtgaagta tacaaggtta ctccatggtt taataaaatg
     3241 gtcgaaaaca aatggttggg cgataaaacc aaacagggtt tttataagaa agaaaaaaca
     3301 cctgatggta aaaggctttc attggtactt gatcctgtca agatggaata ccgggaagca
     3361 cagaagcctg aatttgaatc agtcagtgct gccaagaagc tgccaactgc tgctgaaaag
     3421 attaaagcaa ttgtatgggg aaaagacaaa gcagctcaat ttgcatggaa gcttactgcg
     3481 tatgatgcta tctatgcagc aaaccgtatc cctgaaatag cagattcaat agttgaaatt
     3541 gacaatgcaa tgacatgggg ttataactta gagttagggc cattccaggc atgggatgct
     3601 attggcgtaa aagagtctgt tgaacgaatg gaaaaagaag ggatgaaggt tcctgaaaat
     3661 gtcaagaaga tgctggcatc aggcgctact tctttctata aagtagaaaa cggcaaagag
     3721 tattactatg atctagtaaa tggcggttac aaagaagtta agaagagtga agctgcaata
     3781 tctattttta acttaaaagg cgccggcaaa gaggttaaga agactaattc aatatcactt
     3841 attgatctgg gtgatggtgt attctgcttt gaattccata gcaagatgaa cgctattaac
     3901 ggtgaaatcg tagatttcat gcttgctaat attgattatg taaaagagaa tggtgttggt
     3961 gctgttatag gcaaccaggc tggcggcatg ccgggtgcat tctcagctgg tggtgacctt
     4021 gcttatatgg gaagccttgc aaaagcaaaa aaatggtcag aaattgataa atttattgct
     4081 aatgttcata cttcacagtt agcagcaaaa tatgcaccaa tacccgttgt tgcagctcct
     4141 tatggcatga cgttaggtgg cggttgcgag gtatgtctta atgctgacag gatagtagca
     4201 catgctgagc tttatatggg tctggttgaa attggtgcag gtctgcttcc tggtggtctt
     4261 ggaatgatga acttatggag aaaatatctt aagtctgttc caaaagctgc aaagattacc
     4321 gatttagcag gtcttttcct gccatgcttt atggcagtag ctcaggctca ggttaccatg
     4381 tcagcttttg aagctcagaa gaaaggattc cttggtgcta aagaccgaat cgtattcaat
     4441 agagatcttt taattggcga agccaagaaa gaagtgctaa gaatggttga tgaaggatac
     4501 gctccaccaa agaaggaaaa atttgcggtt cttggccgtg aagctcaggg tatggtagat
     4561 gcagaaatgc tcaatatgcg aataggcatg tacattactc ctcacatgga atttatagca
     4621 aagaagattg catgggtaat gtcaggtggt gatgtgccaa gtggcatgga aattgatgaa
     4681 gcacactggt tgaagttaga gcgagaagcc tttgttgaat tgtggcagac agaaaatacc
     4741 cagaagatgg ctgagcatat cttacaaact ggcaaaccat tatttattta gtcatagaaa
     4801 atctatgcaa acaggaggaa aatttttatg agagatgcat atatcgtaca ggctgtcaga
     4861 acacctgggt gccgtagagg caaaggtgct tttgctcaga cacgacccga agatttgctt
     4921 gtcactgctc tacagggtgt aatcgaacga gcaaaggttg acaagaaagt cgttgatgat
     4981 attatggtcg gttgtgcttt ccctgaagct gaacaggggt taaacgttgg tcgtatagct
     5041 gctcagatgg caggattccc cgatgataca tgtggtgcaa cagttaaccg attttgtgct
     5101 tctggtctgg aagccatagc aatatcaaca atgcgtattc aagccggatg gtgtgatgta
     5161 gctataggtg ctggtttaga atccatgtcg atagtcccca tgggaggcaa tatgccacgc
     5221 cctcatccct actgggcaaa gcagaatccc gacgtttata tctcaatggg aataacagca
     5281 gaaaatgttg ccaacaggta taaaattacc cgtcagatgc aggatgaatt tgcctatcat
     5341 tcacagatga aagctgctga agctcaaaag aacaagctgt acaatgaaat tgttccaaca
     5401 aaggctacta agtttgttcc ccagcctgat ggcactatta agaaagaaga atttatgcag
     5461 gactttgatg acggtgttcg ttcagatact acaattgaag ggcttggaaa actaaaacca
     5521 gcctttgcag catttggttc agttactgct ggcaactctt ctcagaccac tgatggttct
     5581 gctgctacac ttcttatgag cgaagaagca tgccgtaaat acggtgtaaa accactggca
     5641 aaagttaaag cttacgttgt tgcaggatgc aaagcagatg aaatgggtgt tggaccagct
     5701 tatgccattc caaaactctt aaagcttgtt ggactggata ttaaagacat aggcatattt
     5761 gagatcaatg aagcatttgc atcacaggca gtttactgtg cacaacagct tggtatagct
     5821 gatccaaaag aatgggcagt tgacagcaaa acacgccgta tcaatgttaa tggcggagct
     5881 atagctttag ggcatccatt aggatgtacc ggtgcaaaat tggctgcaca gatcgttaat
     5941 atgatgacta aaaacggtat taaatacggt attgagtcaa tgtgtatcgg cggcggtatg
     6001 ggtgctgctg ca
//
LOCUS       DAOSOX010000147         5832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13851_length_5832_cov_8.080492, whole genome shotgun sequence.
ACCESSION   DAOSOX010000147 DAOSOX010000000
VERSION     DAOSOX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13851_length_5832_cov_8.080492"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLX22_09630"
     CDS             complement(<1..452)
                     /locus_tag="PLX22_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT20196.1"
                     /translation="MEHKIIVSAKDIVKTFVMKKGALGKHGLVKAVDRVSLSIPEGNT
                     YGLVGESGCGKTTLARALLRLIEPDSGEIMIRNTPIHTLQKKELRQFRKNMQIVFQDP
                     YGSLNPRMTVEKIIAEALKIHCQLTKKDIVSRISELLAMVGLDKHFASR"
     gene            756..1784
                     /locus_tag="PLX22_09635"
     CDS             756..1784
                     /locus_tag="PLX22_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20197.1"
                     /translation="MQKIFRLLSLLIVASIVITSSGCATVYRQQKKKINENYQEGIQL
                     YKQGDYKNAKEHFETVLSIDPQHSGAKQYLIITNEALQKRTKKYYNAGIQYKRKGDLE
                     NALIQFLQAQQRDPDYKDVKQQISNIRSSKYATKKYNTYYSTAKKQYEKKRYIAAYKN
                     CNKAELFDPNSLDLITLKARIKNQLDNNSYPYTSKAEAAKTKNPALAKKYCNKALAVN
                     PWDEKAQAIAKDIKRIEKLNDLYANGEKAYKKGDYVTAYRAFKQIDNNEPGYRNTANY
                     LATIKTKLEANINTYYQNGVTYYEQDNFKAAIAEWDIVLLINPDHQKAREYRERAVTK
                     LELQQSLQ"
     gene            complement(1799..2989)
                     /locus_tag="PLX22_09640"
     CDS             complement(1799..2989)
                     /locus_tag="PLX22_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT20198.1"
                     /translation="MFINFFFNLKAQGVPVSIHEWMALHEALVLNLNDVSLTKFYYMA
                     RAILVKNEVLYDRYDIAFLDTFKDVETTDELLEQILEGLKKVKELHLTEEEKRQVEKL
                     NLDEVLRNFEEQLRQGHYKNHVGGNKAIGTGGRSTQGAWGYNPAGIRIGQGVSRHKSA
                     VQIAEKREFKNYSNQIILDTRQMRVALSHLRSLLPIGPEEHLDVEQTVDATSKNAGEL
                     ELIWKKKEKKAAKLILLMDAGGSMTPYARLVERLFSAASSQISKLKYFYFHNCIYQDL
                     WKDIERSESISTVDFLKNTDKDYKLIIVGDAEMAPSELTWANGAIDYWYHNDIPGIVW
                     LQRLRETFKYSLWLNPIPQRSWDYIQTTRMIKNIFPMFELTLEGLDEAIKHLISGKVL
                     QKAV"
     gene            complement(2979..3833)
                     /locus_tag="PLX22_09645"
     CDS             complement(2979..3833)
                     /locus_tag="PLX22_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT20199.1"
                     /translation="MNYFTGTDEYVLSQELKDNVNVAIALGRPLLVKGEPGTGKTLLA
                     HSIARGLGKRLLVWNIKSTTKAQEGLYVYDTVQRLNDARFGGKDVSNIKQYIKLGKLG
                     ESFASEEQVVLLIDEIDKADIEFPNDLLHELDIMNFYIPETGETIAAQHRPIVIITSN
                     SEKELPDPFLRRCVFHYIAFPDEELMERIVRVHFPDIEKKLLRECLKKFYWIRQFDML
                     RKKPSTSELVDWIQALKIGGVSIKKIEDEIPFIGTLLKKKEDIDVVANSGKTHYAYNS
                     SIRTRDVY"
     gene            complement(3946..4341)
                     /locus_tag="PLX22_09650"
     CDS             complement(3946..4341)
                     /locus_tag="PLX22_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT20200.1"
                     /translation="MVSFQVSTGRKEELIDITAKVAEAVSKSSIDSGLCVVYVPHTTA
                     AVTINENADPAVSKDILLGLSHMKLESLHFAHLEGNSPAHIKSSLIGCSVSVIVEKGR
                     LQLGTWQGIFFCEFDGPRTRKVMVQCIGT"
     assembly_gap    4601..4700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4701..>5832
                     /locus_tag="PLX22_09655"
     CDS             <4701..>5832
                     /locus_tag="PLX22_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20201.1"
                     /translation="HRFCYNVIHDYKTALLQFQVQYFYLFNPQYSKQIIAKLDAVNPL
                     YYELIVMISNIFDRTRFTEIEEIIQQNEPVSTAKLKNHIIPIYRSLYILYPYIELIEL
                     AYRTAYDTLGTIEKQKSSVYYERKKFIRNSLYTTFYKLLPRLHIIICYYYNVYIDITN
                     PYVQTIMQITQDVLPGKRKAIQSNQEASQQQEAETQEQTTQTPPHIIKGISLMNISTN
                     DIMTLFKNNSSVQPSPADKAAITYVLFKKFDDEFSIVLTTNKIKYTIQFTESGKIDYK
                     NRLADLYNELRKITESFQEYMHILETYERIRSEKPMSNAQYIEYTKILTQLEKKKKVQ
                     SKNMRMVTKSIMDAITAELDILIKDMDEEHKIVDNPQDFLEFD"
BASE COUNT         1913 a         1193 c          924 g         1702 t
ORIGIN      
        1 cgtgatgcaa agtgtttatc aagtccaacc attgccaata attcagatat cctgctgaca
       61 atatcttttt ttgtcaattg gcaatgaatc ttcagcgctt cagcaattat cttttcaaca
      121 gtcatacgag gattcagcga accataggga tcctgaaaaa ctatctgcat attctttcta
      181 aactgccgta attctttttt ttgtaatgta tggataggag tattccgtat cattatttct
      241 ccgctatcag gttcaatgag gcggagtaat gcacgggcta atgttgtttt accgcatcca
      301 ctttcgccaa ccagcccata ggtattccct tcaggtatgg ataagcttac cctatcaaca
      361 gcttttacaa gcccatgctt gcccaacgct ccttttttca ttacaaaggt tttaactata
      421 tcctttgcac ttacaataat tttatgttcc attgattatg ctattctttt ctacactgtt
      481 attatgcgtt cctgccatgt tgaatcatag tctgccttca gaacaggatc atctccgcat
      541 cgattgctta acatcaaatc atgaatcaga gttcaggata actatcgcca aaaattttta
      601 ttcatttaca ttgcttcatt gcagcaatcc acaatagtta tgcaaaataa tcaattgtca
      661 accctaattt attgtgaaaa tttttacttg catatatagc atatataatt ttagtatata
      721 tctataaaaa ataagtacag tcatggggtg ctggtatgca aaaaatattc cgattacttt
      781 cattattgat agttgcaagt attgtcataa ccagttcagg atgcgcaacg gtatacaggc
      841 agcaaaagaa gaagataaat gaaaactatc aggaaggtat ccagctctat aagcaaggtg
      901 actataaaaa tgcaaaagaa cattttgaaa cggtgctttc tattgatcct cagcattcag
      961 gggcaaagca atatttgatc attacaaacg aagccctgca aaaacggact aaaaaatatt
     1021 ataatgctgg tatacaatat aaacgtaaag gtgatttaga aaatgcgttg attcaatttt
     1081 tgcaagccca gcagcgcgat cctgattata aagatgttaa acaacaaatt tcaaatatac
     1141 gcagttcaaa gtatgcaaca aaaaaatata atacttatta ctctactgca aagaaacaat
     1201 atgaaaaaaa gagatatatc gctgcttata aaaactgtaa taaagccgag ctatttgatc
     1261 ccaatagtct tgaccttata acattaaagg caaggataaa aaaccaactt gataacaatt
     1321 catatcctta cacatcaaaa gcagaagcag ctaagacaaa aaatcctgct cttgcaaaaa
     1381 aatattgtaa taaagcgctt gctgtcaatc cgtgggatga aaaagctcaa gctatagcaa
     1441 aagatattaa acggattgaa aagctcaatg atctctatgc aaatggtgaa aaagcgtaca
     1501 aaaaaggaga ttatgtaaca gcctaccggg catttaaaca aattgacaac aatgaaccgg
     1561 gatacaggaa taccgcaaat tatcttgcaa ccattaaaac caaattagag gcaaacataa
     1621 acacctacta tcaaaatggc gttacctact atgagcagga taactttaag gctgctattg
     1681 ccgaatggga tatagtactc cttattaacc ctgatcacca aaaagcacgt gaatatcgtg
     1741 aacgggcagt aacaaaattg gaattgcaac aatcacttca gtaaaaaggc ataacctttt
     1801 acacagcttt ttgcaatacc ttgcctgata ttaaatgctt gattgcttca tcaagccctt
     1861 ccagggtaag ttcaaacatg gggaatatat tttttatcat ccgcgtagtc tggatataat
     1921 cccatgaacg ctgcgggata gggttaagcc atagtgaata cttaaatgtt tcacgcaacc
     1981 gctgcagcca tacaatgcca ggaatatcat tatggtacca ataatcaata gcaccgtttg
     2041 cccatgtcag ttctgatggc gccatttcag catcgcctac aatgataagc ttatagtctt
     2101 tatcggtatt ctttaaaaag tcaacagtgc ttatgctctc tgagcgttca atatccttcc
     2161 ataaatcctg ataaatacaa ttgtgaaaat aaaagtattt taatttactg atttgcgaag
     2221 atgctgctga aaaaagcctt tcaaccagcc tggcataagg agtcattgaa ccgcccgcat
     2281 ccatgagtaa aataagcttt gctgcttttt tttctttctt tttccatata agctccagct
     2341 cacctgcatt tttactggta gcatcaacgg tttgctcaac atccagatgt tcttcggggc
     2401 caataggcaa taatgagcgc agatgcgaca gcgccaccct catctgccgt gtatcaagga
     2461 tgatctgatt ggaatagttt ttaaattcac gcttttcagc aatctgcact gcgcttttat
     2521 gccgtgatac accctgccca attctgatac ctgcaggatt atatccccat gccccctggg
     2581 tggaacgccc gccagtacca atggctttat tgccaccaac atgattttta taatgtccct
     2641 gccgcagttg ttcctcaaaa ttgcgcagaa cttcatctaa attgagcttt tcaacctgac
     2701 gcttttcctc ctcggttaga tgaagttcct ttaccttttt taagccttct aaaatttgtt
     2761 caagcaactc atcagtggtt tcaacatcct taaatgtatc aagaaatgca atatcgtatc
     2821 ggtcatacaa tacttcattc ttcacaagaa tagcccgtgc catataataa aatttagtca
     2881 ggcttacatc gtttagatta agcaccagcg cctcatggag agccatccac tcatggatgg
     2941 aaacaggcac gccctgcgct ttaaggttaa agaaaaaatt aataaacatc gcgcgtcctt
     3001 atgctgctgt tgtaggcata atgtgttttg cccgagtttg ctacaacatc aatatcttct
     3061 tttttcttta agagtgttcc aataaatggt atctcatcct ctattttttt tattgataca
     3121 ccgcctattt taagagcctg tatccagtca acaagttcgc ttgttgatgg ttttttccgc
     3181 aacatgtcaa actgccgaat ccagtaaaat ttcttcaaac attcacggag cagcttcttc
     3241 tctatatccg gaaaatgtac ccgtacaatc ctctccataa gctcttcatc aggaaatgca
     3301 atatagtgaa atacacagcg ccgtaaaaat ggatcgggca gttccttttc gctattggag
     3361 gttataatga caattggccg gtgctgtgct gctatcgtct cgcctgtttc aggaatataa
     3421 aaattcatga tatcaagctc gtgcagaagg tcattgggga attcaatatc cgccttgtct
     3481 atctcatcta ttaaaagaac cacctgctct tctgatgcaa atgattcgcc caacttcccc
     3541 agcttaatat attgtttaat gttggaaaca tccttgccac caaaccgcgc atcgtttaaa
     3601 cgctgcaccg tatcatatac atataaacct tcctgagcct ttgttgttga ttttatattc
     3661 caaaccaata aacgttttcc caatcctctg gcaatggaat gcgccaggag tgttttcccc
     3721 gtccctggct ccccttttac caataatggc ctgcccaatg ctatagcaac atttacatta
     3781 tccttcaatt cctgcgaaag aacatactca tcagtaccgg taaaataatt catcgtgcac
     3841 ctcatcatat tttatacgat agttctttat actatttata ctatttatac tatcctattg
     3901 tactattgta gtacaggaaa gcataaaaaa caaaaaatta cactgttatg ttccaataca
     3961 ttgcaccata accttacggg tgcgcggacc atcaaattca caaaaaaata tcccctgcca
     4021 tgttccaagc tgtaagcgcc ctttctcgac aatgacactt actgagcatc caatcagtga
     4081 cgatttaata tgcgcagggg aatttccttc taaatgcgca aaatgtaacg actctaattt
     4141 catgtgcgat agtcccagta aaatgtcctt ggacactgca gggtccgcat tttcgttaat
     4201 agtgactgcc gctgttgtat gaggaacata cacaacacat aagccgctat caatagatga
     4261 tttactaaca gcctccgcca ctttagcagt gatatcaatg agttcttctt ttctgcctgt
     4321 tgaaacctga aatgaaacca ttaatgtgcc tcaattatta tagtatagta tataatagta
     4381 attctttaat acaatgtaac aattaataca gttatagcgt tttttttaca agattttttc
     4441 agattgttat ccatagcagt gcataatgca accacatata aaataaaacc tgtaatgttg
     4501 ttgacataca cattacatgg tatgtatatt tattaataga gcaaagcatc atgaaaaaag
     4561 tagatagaac aaaagaaaac atcaaggttg acgaattaga nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn gcatcgattt tgttacaacg taatacatga ttataaaaca
     4741 gcactgctgc aatttcaggt gcaatatttt tatctattta atccacaata cagcaagcag
     4801 attatagcaa aattagatgc tgtcaacccc ctttattatg aacttatagt aatgatatcc
     4861 aatatatttg acagaacgcg attcaccgag atcgaagaaa taatccagca aaacgagccg
     4921 gtaagtacag caaaacttaa aaatcacatc atcccaatat acagaagttt gtatattctt
     4981 tatccatata ttgaattaat tgaactggca taccgtactg catacgatac gctgggaact
     5041 atcgaaaaac aaaaatcttc agtatactat gaaaggaaaa aatttatccg caacagcctg
     5101 tacacaacat tctataaatt acttccgcgt cttcacataa taatctgtta ttactacaat
     5161 gtctacattg acataacaaa tccctatgta caaactatca tgcaaataac tcaggatgtt
     5221 cttcccggaa aaagaaaagc aatacagtca aaccaagagg catctcagca acaagaagct
     5281 gaaacacagg agcaaaccac ccaaacacct ccgcatatca ttaaagggat ttcccttatg
     5341 aatatatcaa ccaatgatat tatgacatta tttaaaaata attccagtgt tcaacccagc
     5401 ccggctgata aggctgctat tacgtatgtg ctttttaaaa aatttgatga tgaattttct
     5461 attgtgctca ctaccaataa aattaaatat actatccaat ttaccgaatc aggcaagatt
     5521 gattataaaa acaggctagc tgacctttac aatgaactgc gaaaaattac tgagagtttt
     5581 caggaatata tgcacatact ggaaacatac gaacgcatac gttcagaaaa acccatgagc
     5641 aatgcacaat atattgaata taccaagata ctgacacaac ttgaaaaaaa gaaaaaagtg
     5701 cagtcaaaaa acatgcgcat ggttaccaaa tcaatcatgg atgcaattac agcagaattg
     5761 gatatcttaa taaaagatat ggatgaggaa cataaaattg ttgataatcc acaggatttt
     5821 cttgagtttg at
//
LOCUS       DAOSOX010000148         5802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13950_length_5802_cov_9.101270, whole genome shotgun sequence.
ACCESSION   DAOSOX010000148 DAOSOX010000000
VERSION     DAOSOX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13950_length_5802_cov_9.101270"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..484
                     /locus_tag="PLX22_09660"
     CDS             56..484
                     /locus_tag="PLX22_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT20202.1"
                     /translation="MRLSTRSRYGVRMMFEFALNYDKGPVYLKDVAAIQGISFKYLSK
                     LVIPLKSAKLLKSVRGVHGGYMLAQRPEDITIRKIVQILEGDVSLVECAENPEACSRY
                     AECVARSIWEKADKAIMDVLNSISLQDMMNEYKKNFFNSV"
     gene            510..1427
                     /gene="cysK"
                     /locus_tag="PLX22_09665"
     CDS             510..1427
                     /gene="cysK"
                     /locus_tag="PLX22_09665"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT20203.1"
                     /translation="MKIYNSVTELIGNTPLVKLSGLAPEGVTIMAKLESANPLSSVKD
                     RIGFAMIRDAEEKGLLHDKTIIVEPTSGNTGIALAFVSAVKGYKLMLTMPDTMSMERR
                     KLLQMLGAEIVLTDGAMGMRGAIEKAQEIVERTTSAFMPQQFNNPANPEIHRKTTAME
                     IWNDTGGNVDVFVAGVGTGGTITGVGEVLKAKKPSVTIIAVEPEKSPVLSGGKPGPHK
                     IQGIGAGFIPQVLNRSIIDEIVTVDDTDAGNTASKLAKVEGILAGISAGAAMWAACKI
                     ASRPESKGKTIVVLLPDSGERYVSTWLFD"
     gene            1658..2941
                     /locus_tag="PLX22_09670"
     CDS             1658..2941
                     /locus_tag="PLX22_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOT20204.1"
                     /translation="MSKNYRIETIALHGGQSLDATRSRAVPVYRTSSYVFDSAKHGAD
                     LFSLKEKGYIYTRMMNPTQDVLEQRVALLEGGSAALALASGTSAIFYSIINICKAGDE
                     FISASNLYGGTYTMFDAILPQFGISSRFVPYDDYGAYEKAVNEKTRAIFVESIGNPTL
                     GVPDFEEIAAIAHKHHLPMIVDNTFATPYHFKPIEHGATIVVHSLTKWLGGHGTAIGG
                     IVVDAGNFDWTDPRFSLYNEPDDGYHGLQFAHDLGDDNALAFILRMRLVPLRNLGAAI
                     SPDNAWLFLQGIETFALRMQRHSDNALKVAQFLQKHSKVAWVRYPGLAGDSCYDNASK
                     YFNNGFGGMVVFGVKSGYAGAVKFINSITLFSHLANVGDAKSLILHPASTSHSQLSEQ
                     QLLASEVTPDLIRLSIGIENIEDIIEALDMALKLC"
     assembly_gap    2969..3068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3182..4039
                     /locus_tag="PLX22_09675"
     CDS             3182..4039
                     /locus_tag="PLX22_09675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOT20205.1"
                     /translation="MLKKSIIIIVAIIVVGILLLGYVFYRATTIPSNNVDAMLKGTIA
                     PDKKIVICFGDSITHGAVSFDYVRFLAEDAALKKFIFVNEGVNSRLVYNLLQVVDKVA
                     AVKPNYVFILIGTNDLKGSLSDDEYNRYNKLWKLPQRPTKQWFADNYKKLVDILKAKT
                     DATIVLISIPPLGENCDSVPFTLAMEYSKIIKQIAAEKKVLYIPFNEILSTELIQHGK
                     KDIPPYTFNEWFMYTSIVQHYLCWRSWNAIADRRGLLFTPDNIHCNERSGMMLTETIK
                     NILLSSNEH"
     gene            complement(4207..4419)
                     /locus_tag="PLX22_09680"
     CDS             complement(4207..4419)
                     /locus_tag="PLX22_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20206.1"
                     /translation="MAADYYSQYHELCNFFTGTIAMKILKIFGLCLLIVIVTTALIIA
                     LWFIRSNTSEVKHSLALEIWDAVNDG"
     gene            4639..>5802
                     /locus_tag="PLX22_09685"
     CDS             4639..>5802
                     /locus_tag="PLX22_09685"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT20207.1"
                     /translation="MKDVVIASIVRTPVGTFGGALKDVSAVDLGVIVVREAVKRAGLS
                     PQQVENVILGNVLQAGLGQNTARQVLIHAGMPHEVPAMTINKVCASGLRSISMAAQII
                     KAGDAEIIVAGGIENMSASPFALPKARWGYRMGDDKIVDIMIKDGLWDAFNQYHMGIT
                     AENVAERWKLTREELDKFSLASQTKAEAAIKSGRFKDEIVPVVVKDKKGEKIFDTDEH
                     PRFGTTLEALSKLKPSFKKDGMVTAGNASGINDGAAAAVVMSADKAKELGIKPLCRIA
                     SYASAGVDPAIMGTGPIPASRKALAKAGWKVQDLDLIEANEAFASQAVVVNKEMGWDI
                     SRVNVNGGAIAIGHPIGASGARILTTLIHEMIKRDARKGIATLCIGGGQGCAVT"
BASE COUNT         1761 a          980 c         1345 g         1616 t
ORIGIN      
        1 cataatatac tactaaaaag gttataatgg tagtataatt aaaaggattc atgctatgcg
       61 actatcaaca cggagcagat atggtgtgcg aatgatgttt gaatttgcac tgaactatga
      121 taaaggacct gtgtatttaa aagatgtggc agctattcag ggtatttcgt ttaaatattt
      181 aagtaagctg gttatcccgt taaagtctgc aaagctgctt aaatcagtgc gaggggtgca
      241 cggtggatat atgctggcgc aacgtcctga agatatcacc atacgcaaaa ttgttcaaat
      301 acttgagggc gatgtttcac ttgttgaatg tgctgaaaat ccggaggcat gcagcaggta
      361 tgctgaatgt gttgctcgca gtatatggga aaaggcggat aaagccatta tggatgtgct
      421 caacagtatt tcgttgcagg atatgatgaa tgaatataaa aagaattttt ttaacagtgt
      481 atgaaatcta atttagtacg agggtagtaa tgaagatata taactcagta acggaactca
      541 ttggcaatac accattggtt aaattgtcag ggttagctcc ggaaggggtt accatcatgg
      601 caaagcttga atcagctaat cccctgtcaa gtgtcaaaga cagaattggt tttgccatga
      661 ttcgcgacgc tgaagaaaag ggattgttgc atgataaaac aattatagta gagccgacaa
      721 gcggcaatac aggcatagcg cttgcatttg tttctgcggt aaaaggatac aagcttatgc
      781 tgaccatgcc cgataccatg agcatggagc gtcgcaaatt attacagatg ctgggcgcag
      841 agattgtcct tactgatggt gccatgggga tgcgtggcgc catagaaaag gcgcaggaga
      901 ttgttgaacg cactacatct gcttttatgc cacaacaatt taataatcct gcaaatcctg
      961 aaatacatag aaaaacaact gccatggaaa tatggaatga tactggcggc aatgttgatg
     1021 tatttgttgc aggtgttggt acagggggaa ccattactgg tgtaggcgaa gtactaaaag
     1081 ctaaaaaacc atcagttacc attatagccg ttgaaccgga aaaatccccg gtgctttccg
     1141 gaggcaaacc cggtccgcat aaaattcagg gtattggtgc agggtttatc cctcaggtac
     1201 tcaaccgctc tattattgat gagatagtta ctgttgatga tactgatgca gggaatactg
     1261 ccagcaaatt agcaaaggtt gagggaatac tggctggtat ttcagcaggg gctgctatgt
     1321 gggcagcttg taaaatagcc agccgtcctg aaagcaaagg caagactata gtagtgctcc
     1381 tgccagattc cggtgagcga tatgtaagca catggctatt tgattgatgg aggccattgc
     1441 aggaagagtt gcccatgggc agcttacagc cacatataat tgatgaaaat ggtctggttt
     1501 aagtcatcct gaaatgtaga aaatcatcct ggattcattt caggatctca gttacaaatt
     1561 ttgattttgg gccaagcacg gaatgacagg gcgtatagtt ctatttttgg agtagaaatt
     1621 tatcttcaat gatgaaataa tgtatgaggg ggatataatg tcaaaaaact atcgcataga
     1681 aacaattgcc cttcatgggg gtcagagcct tgatgcaaca aggtctcggg cggtgccggt
     1741 atatcgtacc agctcatatg tatttgattc agcaaaacac ggtgcagatc ttttttcact
     1801 caaagaaaaa ggctatatct atacccgaat gatgaatcca actcaggatg tccttgaaca
     1861 gcgggttgca ttgttagaag gcggtagtgc ggcattagca ttagcttcag ggacatcggc
     1921 aatattttac agcattatca atatatgtaa agcaggtgat gagttcataa gtgcaagtaa
     1981 cctgtatggt ggcacctata ccatgtttga tgcaattctg ccacagtttg gcatttcaag
     2041 ccgttttgtc ccgtatgatg attatggtgc ctatgaaaaa gctgttaatg aaaaaacacg
     2101 ggctatcttt gttgaatcaa taggaaatcc aaccttaggg gtacctgatt ttgaagaaat
     2161 agcagcgatt gcgcacaagc atcatctccc aatgattgtt gataatacat ttgcaactcc
     2221 gtatcatttc aaacccattg aacatggtgc caccatcgtt gttcattcgc ttaccaagtg
     2281 gctgggaggg catggcaccg ccattggtgg cattgtggtt gatgcgggta actttgattg
     2341 gactgatccc agattttctt tgtataatga gcctgatgat ggctatcatg gattacaatt
     2401 tgcacacgat cttggcgatg acaatgctct tgcatttatt cttagaatgc ggctggtacc
     2461 gcttcgcaat ttaggtgctg ccatctctcc tgataacgca tggctatttt tgcagggaat
     2521 tgaaacattt gcactgcgca tgcagcgtca tagcgataat gctttgaagg tagctcaatt
     2581 tttgcaaaaa cattcaaagg tggcatgggt acgctatcca gggctggctg gcgatagttg
     2641 ttatgataat gcttcaaagt attttaacaa tggatttggc ggtatggtgg tttttggtgt
     2701 caaaagcgga tacgccggtg cagtaaagtt tatcaattcc attacgcttt tttcgcatct
     2761 ggcaaatgtt ggcgatgcaa agagtttgat attgcatccc gcaagcacat cccattcgca
     2821 gttgtctgag cagcagctgc tagcatcaga ggtgaccccc gatcttatcc gcctttccat
     2881 cggaattgaa aatatagaag atatcattga agctcttgat atggctttga agctgtgttg
     2941 aaggaatagt ggagcgatac cgcgattgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnat agtttgtgac tctttaaaaa atcatttgca aatatgttgt tataggctta
     3121 ttatgatagg gtaacctatc atcagtcatg gtttactaca tgcatcatga ggaggaaaag
     3181 tatgctcaag aagtcaatca tcatcatagt ggcaatcatt gttgttggta tactgctgct
     3241 gggatatgtc ttttataggg caactacaat accttcaaat aatgttgatg ccatgctcaa
     3301 gggaactatc gccccggata aaaaaattgt tatttgtttt ggcgatagta ttactcatgg
     3361 ggctgttagt tttgattatg taaggttttt agcggaagat gctgcattga agaaatttat
     3421 ttttgtcaat gagggtgtta acagcaggct ggtgtacaac cttttacagg ttgttgataa
     3481 agtagcagca gtaaagccga actatgtatt tattttaatt ggaacaaacg atctgaaggg
     3541 atcgctttcg gatgatgaat ataataggta caataaatta tggaagcttc cccagaggcc
     3601 aacaaagcag tggtttgccg acaattataa aaaactggtt gatatcctga aagcaaaaac
     3661 tgatgctact atagtgctta tctccatacc gccattgggt gaaaattgtg acagtgttcc
     3721 ctttacgctg gcgatggagt actcaaagat tataaaacag atagcagcag aaaaaaaagt
     3781 gctctatata ccgttcaatg aaatcctttc cactgaactt atacaacatg gcaaaaagga
     3841 tataccacca tacacattca atgaatggtt tatgtataca tcaattgtgc aacattatct
     3901 ttgttggcgt agctggaatg ctatcgcaga tagaagaggt ttgctgttta cccctgacaa
     3961 tatccattgc aatgaacgaa gcggtatgat gcttacagaa acaataaaaa atattttact
     4021 atccagcaac gaacattaat aacattaatg tttattgcta ttgatatgct taagattgca
     4081 gaggcgttat accacgcttt tgcatgaatg taccctctac ttgataaata tgtgtacaca
     4141 aaaaatagag agaggattgt agtcattaat agtaaaaata tacaccgtta gaatgtatgg
     4201 ctaaaatcaa ccatcattga cggcatccca tatttcgagt gcaagtgaat gtttaacctc
     4261 tgaagtattg gaacgtataa accataatgc tatgatgagt gctgttgtga caataacaat
     4321 caacaaacaa agaccaaaaa ttttaagtat tttcatggcg atagttcctg taaaaaaatt
     4381 gcacaactca tggtactgag aatagtaatc ggcagccatg acctgctatc atgcaagtac
     4441 tcaaacacta cggtatagat gcagtaagaa atacgatacg tgagtactta ctcgatacgc
     4501 atacttacgt attgtcaata aatattattt tttctttaca acatggcaac agtttattag
     4561 tattacatca attgtacaga ttgttgtaat aattttataa taattaatat gaaaaatttt
     4621 taataatgag aggtctttat gaaggatgtt gtaatagcgt caatagtgcg aacaccagta
     4681 ggtacttttg gcggagcatt gaaggatgtt tcggcagttg atctgggggt tattgtagtc
     4741 agggaagctg taaaacgagc cgggctgtca cctcaacagg tagagaatgt tatactggga
     4801 aatgtgttgc aggcaggttt gggacagaat acagccaggc aggtattgat ccatgctggt
     4861 atgcctcatg aagtgccggc tatgactatc aataaggtat gcgcttccgg gcttagatcc
     4921 atcagtatgg ctgctcagat tattaaagca ggcgatgcag aaattattgt tgccggtggg
     4981 atagaaaata tgagcgcttc cccgtttgct ttaccaaaag cacggtgggg atatagaatg
     5041 ggtgacgata aaatagttga tatcatgata aaagatggtt tgtgggatgc atttaatcaa
     5101 tatcatatgg gaataacagc agaaaatgtg gctgagcgct ggaagctgac tcgtgaggaa
     5161 ttagataaat tttcactggc aagccagaca aaggcagagg ctgccattaa atctggcagg
     5221 tttaaagatg agatagtccc tgttgtagta aaggataaaa aaggagaaaa aatttttgat
     5281 actgatgaac atccccgctt tggaacaaca ctggaagcat tatcaaaatt aaagccatca
     5341 tttaaaaagg atggtatggt aaccgcaggg aacgcaagcg ggattaacga tggcgcagcg
     5401 gctgcagtag tcatgtcagc agataaagca aaagagctgg ggattaagcc tttatgccgt
     5461 attgcatcgt acgcgtcagc aggtgttgat cctgcaatca tggggaccgg tccaatacct
     5521 gcaagcagga aagctcttgc aaaagcagga tggaaagtgc aggatttaga tttaattgaa
     5581 gcaaatgagg cttttgcatc tcaggctgtg gttgttaata aagagatggg atgggatata
     5641 tcaagagtca atgttaatgg aggagctatc gcaataggac atcccattgg tgcatcgggt
     5701 gcacgaatat taacaacact gattcatgaa atgataaaac gtgatgccag aaaaggcata
     5761 gcaacattat gtataggtgg cgggcagggt tgtgcagtaa ca
//
LOCUS       DAOSOX010000149         5797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_13972_length_5797_cov_8.818878, whole genome shotgun sequence.
ACCESSION   DAOSOX010000149 DAOSOX010000000
VERSION     DAOSOX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13972_length_5797_cov_8.818878"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1481)
                     /locus_tag="PLX22_09690"
     CDS             complement(<1..>1481)
                     /locus_tag="PLX22_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOT20208.1"
                     /translation="LKAGFADDNKQFNYFVNFLQMYADKVTHYPMNISERIILYCKDT
                     GNKSIPNAKVKVYAGNKLIVTGKTYADGSFMFYPSEYAANYKKYTITFEAFNTKAEIA
                     IDRQGKREHTVIMKAQRPQYKNVPLDILFVFDTTGSMGEEIARLKKTIEIINMNIASL
                     TAKPAVRFGMVLYKDKGDEYVTKVIPLTSNLNEFQDELAKVFAGGGGDGPEDLQSALY
                     DTVKKVKWNNNGIRLAFVITDAEPHLDYGQQYTYVNAAHDAKRDAIKIYSIGTGGLPL
                     MGELVLRQISQYTHARYIFLTYGESGESEGGKEGSVSHHTGANFQTDKLETIIIRFAK
                     EELAYLTDQPLEEGEAYFDAKKVEDETNEQTLQKLFDMAIAQLIDYSAINLQKGEPTS
                     VLPIKSSDETLATNAEYFTEQLSVALGKNKVFTQVERKNMQQILHEIGLGQTGIVDEA
                     SAAKAGKLLGAKMLVTGTLYQKKDSYELFLKLIRVESAEVLSAT"
     assembly_gap    1482..1581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1582..2426)
                     /locus_tag="PLX22_09695"
     CDS             complement(<1582..2426)
                     /locus_tag="PLX22_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT20209.1"
                     /translation="MPKKAFTTAKQKNDCIDAIINEMVNRSAFLILGHTNPDEDCVAS
                     MIAFALLARKFDKEVKIFTSKKIPQNFKYLVDIAVYNRIDVVMAIPRTFMADAIVICD
                     TPKPSMIDANRKVQSMLRNPDVRKIEIDHHMGADSDYAGDAGYCLVTEASSASELVGQ
                     IAIKLRAKKEILKKYVILDPLSRNLVLAILTGIIGDSQMGRYLKSSREKRMYRLFSNL
                     FNTILNTATIKEGNITNMDQIFEQLKQASENERRCFQYIKKKSKLYKWVGYAVLSEED
                     MHYLY"
     gene            2765..>3477
                     /locus_tag="PLX22_09700"
     CDS             2765..>3477
                     /locus_tag="PLX22_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20210.1"
                     /translation="MKKTPIHIIVIFIAIGLTVPFSFALQKDDDTPYKKGVKYFYQQK
                     FEIAELLFQEELKNNPENALAYSYLGDIFLYKKRYDGALQLYIKALELNPKSAEDAFR
                     IGQVHYYKKQPEEAINYFQKAFSLNPQLKFAYYHIGLTYLMLLRDKEKTIESWEKFLA
                     IAPEDPQYEKIKRVIELLRDPKFVLPPPGSDVSIEEALHLGGITLDEVQREAQDKKAG
                     HETKKTKNKLEEIYRDDEL"
     assembly_gap    3478..3577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3808..>5797
                     /locus_tag="PLX22_09705"
     CDS             3808..>5797
                     /locus_tag="PLX22_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20211.1"
                     /translation="MAYYYDRYRYVRIAGIALCIILLLFSGYYLYTKLSKSSLQDIVD
                     IRQKQIELYEKLLETNPSDVETLIKLGNLYKEIGNIGKAIGYYNKALAIEPYNYNALN
                     ELGHAYALQGDYENAIKTLELAKKYFPKYPQAYNKLGNVYDTQEKLEKALKEYKSAIA
                     VAPAYQESYWNIAKQRMKKGDYKGAEAILKKGIKANPNDAEGYYNLGNLYMRKKNYAK
                     AIPQYQKAIALSPATGKYYRALGDAYLAQNKIPEAIGAYSTALAKDPGDALSAEKLGD
                     IYASQGNWGKAVDYYRQALNYNSNDQNLKNKYANALSKLQKPVFAQKEPSPVTIASNE
                     KNDTPAQALVRDKDLSDATQQPSSVNAVNESALKWRELGDKYRAEKKYDEALKAYAKA
                     TQMDKTDDYSHHWMGRIYYHNHIDDEAKKSFQNSIIANPKNADSHYHLGLIYYTEGKY
                     NAAAIEFNKAIEEKPVFPKAYYARGLAYYKMNNLQGAIKDLKQSIEQDPNLDRAYFNL
                     GDIYLKQKNYKVALDYFTKDRELRPNNPDTLFKIAETYHEMKSYSNAETFYNKTLEAD
                     PEYFQALFNLGLISATKQNYAQAVSLYTRALSIKNDDPPTLYELGKAYEGLGDDNKAI
                     EYYSLAITKNPNYTKAYINVGNLYKKNKLHDKAIEQFKQ"
BASE COUNT         1830 a         1096 c         1004 g         1667 t
ORIGIN      
        1 gtggcagata aaacctcggc gctttccacc cttataagtt tcaagaacag ttcatagctg
       61 tcttttttct ggtacagtgt accggtaaca agcatctttg cacccagaag cttcccggct
      121 tttgcggcag atgcctcgtc tacaatgcca gtttgtccaa gcccaatctc atgtaatatc
      181 tgctgcatat ttttacgctc aacctgcgta aacaccttat ttttacccag tgctacggac
      241 aactgctcgg taaaatattc agcattggtt gcaagtgttt catcagatga tttaatgggc
      301 aataccgatg ttggttcacc cttttgaagg tttatggctg aataatcaat taattgagct
      361 atcgccatat caaaaagttt ttgcagggtt tgctcattgg tttcatcctc aacctttttt
      421 gcatcaaaat atgcttcacc ttcctcaaga ggctgatcgg taagatatgc cagctcttct
      481 ttagcaaatc gtatgatgat tgtttccagc ttatccgtct ggaaattagc accggtgtga
      541 tggcttacac taccttcttt acctccctca ctttccccgc tttcaccata ggttaaaaat
      601 atataccgcg catgagtata ctgggaaatc tgccgtaata ccaattcacc catcagggga
      661 agcccgccgg tacctatgga atatatcttt atagcatccc ttttagcatc atgagcagca
      721 ttaacatagg tatattgttg cccataatct aaatgaggtt cagcatcggt gataacaaaa
      781 gcaaggcgta tcccattgtt attccatttt acttttttta cggtatcata aagagcgctt
      841 tgcaaatcct ccgggccatc accgcctccg ccggcaaaaa ccttagcaag ctcatcctga
      901 aattcattta aattactggt aagtgggata acttttgtga catactcatc acctttatct
      961 ttatagagca ccatcccaaa ccttacagca ggctttgcag ttaatgaggc aatattcata
     1021 ttgataattt cgatagtctt ttttaaacgg gcaatctcct cgcccatgct tcctgttgta
     1081 tcaaatacaa aaagaatatc aagaggaaca tttttgtact gcggccgctg tgctttcatt
     1141 ataacagtgt gctcacgttt tccctgtcta tcaatggcga tttcagcctt tgtattgaaa
     1201 gcttcaaagg taatggtata ttttttatag tttgcagcat attctgatgg ataaaacata
     1261 aaggacccat cagcatacgt tttccctgtt acaatcagtt tgttgccggc atatacctta
     1321 acctttgcat ttggtatgga tttattccct gtatccttac aatagagtat tatcctttcg
     1381 gaaatgttca tgggataatg ggtaacctta tcagcataca tctgtaaaaa gtttacaaaa
     1441 taattaaact gcttattatc atcagcaaat cctgctttta annnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn ntcataaagg tagtgcatat cctcttcgga taaaacagca
     1621 tacccaaccc atttatagag cttactcttt ttcttaatgt actgaaaaca tctcctttca
     1681 ttttctgaag cctgtttcaa ctgttcaaaa atctgatcca tattggtgat attgccttct
     1741 tttatggttg cagtattcag gatagtattg aaaaggttgc tgaacagcct gtacatcctt
     1801 ttttcacggg atgacttaag atagcgcccc atttgcgaat cgccaataat accggttaaa
     1861 atagccagca caaggttacg tgaaagggga tctagtatta catatttttt taaaatctcc
     1921 ttctttgccc tcagcttaat ggctatttgt cccaccagct cacttgcgga agaagcctcg
     1981 gtaacaaggc agtatccggc atcacctgcg taatcactat cagcacccat atggtggtca
     2041 atctctatct tccgtacatc gggattacgt aacatgctct gtactttacg gttggcatca
     2101 atcatggaag gcttgggtgt atcacagata actatcgcat cagccataaa agtccttgga
     2161 atggccatta caacatctat acggttatat acagcaatat caacaagata tttaaaattt
     2221 tgcggtatct tctttgaagt aaaaatcttc acctctttat caaatttcct tgcaagcagg
     2281 gcaaatgcaa tcattgacgc gacacaatcc tcgtcaggat ttgtgtgtcc taaaataagg
     2341 aatgcactgc ggttaaccat ctcattaatg attgcatcaa tgcagtcatt tttttgtttt
     2401 gctgttgtaa aagctttttt aggcataggt attttttaaa atttttaatg gcgctgatca
     2461 taatacaatc ttatgaaaat gtcaacagca tattttttta ttttttccag tacttgactc
     2521 agggttgatt attggttatt ttttaaaggt aaggttttat tacattttca ttatcctatt
     2581 gagtattatt gcttttttat gaatcttatt ttgaggaggt acgatggatt ttgatattaa
     2641 gcgattttta cttgccataa cgtctttgat tattttcttt atttttgcca tgcttttttg
     2701 ttaatgtgta caatcggttt ttactcaatg agtaccctat aaagtgacga tatagtaaac
     2761 aattatgaaa aaaacaccca tccacatcat agtcattttc atagcaattg gattaactgt
     2821 acctttttca tttgctctac aaaaagatga cgatactcct tacaaaaaag gcgtgaaata
     2881 tttttaccag caaaaatttg agattgcaga acttctgttc caggaggagt taaaaaataa
     2941 tcctgaaaat gcgcttgcct attcatattt gggcgatatc tttttatata agaagcgtta
     3001 tgatggtgca ttgcagcttt atataaaagc gctggagtta aatccaaaaa gtgcggagga
     3061 tgcatttcgc atagggcagg ttcactatta caaaaaacag cctgaagaag ctatcaatta
     3121 ttttcaaaaa gcctttagcc ttaatcctca actaaaattt gcctattatc acatagggct
     3181 tacctatctt atgctgttgc gggacaagga aaaaacaatt gagtcatggg aaaaattttt
     3241 ggcgatagct cctgaagatc cacaatatga aaagataaaa agagttattg aattattgag
     3301 ggacccaaag tttgttttgc cgccgccagg aagcgatgtt tctattgaag aggcattgca
     3361 tctgggagga ataacgcttg acgaagtaca gcgcgaagct caggataaaa aagcagggca
     3421 tgaaacaaaa aagacaaaga ataaattaga agagatatat cgtgatgatg aattatannn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa caaaaaagac aaagaataaa
     3601 ttagaagaga tatatcgtga tgatgaatta taaatgccgg gatgatacca tcgtaatcaa
     3661 gcaggatgag aatacttact gtttgtcatc ctgaaccata tcctgaactg ttttcaggat
     3721 catttcagga tctaatgtaa gcaaaagaga ttccggggca agcccggaat ggcaggggta
     3781 tataattctt ttttatagga gttcatgatg gcatattatt atgaccgtta ccgttatgta
     3841 agaattgcag ggatagctct ttgtatcatt ttacttcttt tttcaggtta ctatctgtac
     3901 acaaaattgt caaagagttc gttacaggat atcgttgata tacgccaaaa acagattgaa
     3961 ctgtacgaaa agctccttga aacaaaccca tctgatgttg aaacactcat caaactggga
     4021 aacctttata aagaaatagg caatattggg aaagctatcg gctactataa caaagcgctg
     4081 gcaatagaac catataacta caatgcactt aatgaattgg ggcatgctta tgcattacag
     4141 ggtgactatg aaaatgctat taaaacgctt gaactggcaa aaaaatattt tccaaaatat
     4201 ccgcaagcct ataataaatt agggaatgtt tatgataccc aggaaaaatt ggaaaaagct
     4261 ctgaaagaat ataaaagcgc tatagcagtt gcacctgcgt atcaggaaag ttactggaac
     4321 attgctaaac aacgcatgaa aaaaggcgat tacaaaggtg ctgaggctat tctaaaaaaa
     4381 ggaattaaag ccaaccccaa tgatgcagaa ggctactata atctgggcaa cctttacatg
     4441 cgtaaaaaaa actatgcaaa agcaatacct caataccaga aagcaattgc actttctcct
     4501 gcaaccggta aatactatcg cgctctgggc gatgcctacc ttgctcagaa caaaatccct
     4561 gaagcaattg gcgcttattc aaccgcactg gcaaaggatc cgggagatgc actttcagca
     4621 gaaaaattag gcgatatcta tgcatcacag ggaaactggg ggaaagctgt agactattac
     4681 cggcaggcgc tgaactataa cagcaatgat caaaatctaa agaataaata tgcaaatgca
     4741 ctttcaaaac tgcaaaaacc agtatttgca caaaaagaac cttccccggt aactatcgcc
     4801 agcaatgaaa aaaatgatac accggcacaa gcacttgtcc gcgataaaga cctttctgat
     4861 gccacacaac aaccatcatc agtaaacgct gttaatgaat ccgcgttaaa atggcgtgag
     4921 ttaggcgata aataccgtgc agaaaaaaaa tacgatgaag cactcaaggc atacgcaaaa
     4981 gcaactcaaa tggataaaac tgatgattac tcacatcact ggatgggcag aatctactac
     5041 cataatcata ttgatgatga agctaaaaaa tcattccaga attcaattat tgcaaatcca
     5101 aaaaatgctg attcacatta tcatttagga ctcatctatt ataccgaagg gaaatataat
     5161 gcggcagcta ttgagtttaa caaagctatt gaagaaaagc cagtttttcc aaaagcatac
     5221 tatgcacgag gtttggctta ctataaaatg aacaatttac agggtgctat aaaagatcta
     5281 aaacaatcca ttgaacagga ccctaactta gacagggcat atttcaatct gggcgacatc
     5341 tacttaaagc aaaaaaatta taaagttgca ctggactatt ttactaaaga cagggagcta
     5401 cgacctaata atcctgacac cctttttaaa attgcagaaa cctaccatga aatgaaatca
     5461 tactcaaatg ctgaaacatt ttacaataaa acactggaag ctgatcctga atattttcag
     5521 gctcttttta acctgggact gatttcggca accaaacaaa attatgcaca ggcagtttct
     5581 ctttatacac gagctttatc cattaaaaat gatgacccac caacactcta tgaactgggt
     5641 aaagcatatg aaggtctggg tgatgataat aaggcaatag aatactattc actggctatc
     5701 accaaaaatc ccaactatac taaagcatat attaatgtag ggaatcttta taaaaagaac
     5761 aaacttcatg ataaggcaat tgaacaattt aaacagg
//
LOCUS       DAOSOX010000150         5740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14162_length_5740_cov_5.370449, whole genome shotgun sequence.
ACCESSION   DAOSOX010000150 DAOSOX010000000
VERSION     DAOSOX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14162_length_5740_cov_5.370449"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLX22_09710"
     CDS             <1..525
                     /locus_tag="PLX22_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgL"
                     /protein_id="HOT20212.1"
                     /translation="NNAGLSVKASLGARNNLVLETTVPHAIWLEDEGSGTFLRDAGMV
                     NPDFPNPPNNVDPTVSVSGMSIFDMVIQLRDDLVRGDQELVGGRDLGLIDMALDNVLR
                     HIAAVGAKENRIEELAKRSEYAKSNILDILAKSEGIDFPETIMNFRWLESVHNYALAV
                     GARSIKPTLMDFLR"
     gene            550..1002
                     /gene="fliW"
                     /locus_tag="PLX22_09715"
     CDS             550..1002
                     /gene="fliW"
                     /locus_tag="PLX22_09715"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOT20213.1"
                     /translation="MVTIATKPFGQIEVDERQIIDFPEGIYGFEDIKKFIILDANEKS
                     PFKWLQAYDEPDLAFVIIRPIDFMMQYELNVMPEDLEDIGAKSPDEVIVFAIVTIPED
                     PSKMTANLQGPVLINPKTRRGKQAISLSDKYTVRHYILEEIKKAQVKR"
     gene            1007..1273
                     /gene="csrA"
                     /locus_tag="PLX22_09720"
     CDS             1007..1273
                     /gene="csrA"
                     /locus_tag="PLX22_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOT20214.1"
                     /translation="MLVLARKVNESIMIGDDIEIVIIDIKGDQVKLGIKAPKSVAVHR
                     KEIYEEIQKENIAAMKSQLKPETLKDITDILQTHKEKNIDNQKK"
     gene            1420..2088
                     /locus_tag="PLX22_09725"
     CDS             1420..2088
                     /locus_tag="PLX22_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT20215.1"
                     /translation="MPDIIVQTNVAYLVGIFFIIFILREIVAFKKILKLKYIFTPLIT
                     YIVIIIALYLSYVRGFTVYNIFIIAGLVFALIADTFLMIEEISFFIHGLFFFLLTHLC
                     YVYALSINYHFGTIDIIIIMIILSIFFLYYLVIHKAKGKVYIPVILYMLVLSLVLLLA
                     IGTFLKNVYPRGAIVTMAAILFAISDGVLAFNQFVHKVPHSTVVTWSLYAPAQLLFAV
                     SCYY"
     gene            2386..3453
                     /locus_tag="PLX22_09730"
     CDS             2386..3453
                     /locus_tag="PLX22_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20216.1"
                     /translation="MKKIFIFILLIFISCGQLIKQSPQKDYERIYKNLTNAESLENPV
                     QKDKLINKAYTLLMQRQAELIKEKKSDTTVAALLAYYYYLKGNYQDASSQVELASSPS
                     GNNQLGAIIQARVLLSTKGKAGAQGALDILRAVEGLDNPMAFIAMGDAYFLKADYDKA
                     RDNYTKALLLNRELQVVAANRLEVISRIKTLSINVAKVSDFILEPTMTRDKLAYILYE
                     IFELQKYIATAKPLDANFIDLEKSQYKSAIVSLRQKGFFSYIQGSAFEPFMVVSRGEM
                     AKLVEDFLVLSRNNEGLRSKFKNESPPFFNDIKNDNEYYNAMRLAVDMGIMTASLSQD
                     AYPDESVTGLQAIMILQKLVK"
     gene            3481..5706
                     /locus_tag="PLX22_09735"
     CDS             3481..5706
                     /locus_tag="PLX22_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20217.1"
                     /translation="MIIKRRLKIFFTIISALVIAAITYAQFTDNAQSDEKNRTSKITY
                     KNYIWIEGENAVSTNFAREPIYNFFCSNKFALQLSKDVDPPQDGYFATYVFYVTHTKE
                     YDFWMGCTPPGSAYKDRPGYASPIEWQIDNGPFQTASAENVYVKSFYGIGGFYWVKIA
                     SGTLNAGKHTLTIRVKQKRSSGWDYYFYVDAILLLPQYSTGIAQLMDFPEVAPKDFAT
                     PGDGILFNAPEFYEKKLRANISDREALFSLVQIYGWLYDYNKAIFLLKKYLEKNPRDL
                     DMRLELAAFYSWSDQLDAAISEYKNIIAIDEKNITARKLLSVLAGWNNRYDEAIKYYQ
                     EIIAIDPENVDAYISLGTQYSWKGEMNRAIATFEKAETIAPSNIEVLEVLGDNYYWAG
                     RAYDAIRQYYRIIDINEKQISAYKKLAKIYLDLGESAKAARIINDAKQVVDLYPELSG
                     ISLDVKDELAKERRALIDSYKEALVKNPDDVNLRKGLVDTYIWNRMYGDAVKEYDALL
                     AYKMFHSIEQAEQKVTRLAIHTVGLQSLMPLIKTIKDDGSIIKKRYAKLIDDKKKGKA
                     VIEPSSLASDKATLEWYNAKLKEIQKELYVYNDITAAFGKDVQTYTAMQAATGWHFES
                     DRIYRYAATAKKLNPQMYQPYKVQGTIDLLYDKPSNAVSSFELVKKLAAGAEFPGLPL
                     AYAQTKKYNEAIALLEESLQSKKVSATYKNDIEQMLSLVKLSKGEVSSQSSLQVTTQK
                     K"
BASE COUNT         1908 a          928 c         1169 g         1735 t
ORIGIN      
        1 aataatgctg gcctgagcgt aaaagcatca ctaggtgcgc gaaacaattt agtgcttgaa
       61 acaacggtac cgcatgctat atggcttgaa gatgaaggca gcgggacatt tttgcgcgat
      121 gctggcatgg taaatcctga ttttcccaat ccgccaaaca atgttgatcc cacggtgtca
      181 gtaagtggta tgtcaatttt tgatatggtt atacagttgc gtgatgacct ggtgcggggt
      241 gatcaggagc ttgttggtgg gcgtgacctg ggacttattg atatggcgct tgataatgtg
      301 ttgcgccaca ttgctgcagt tggtgcaaaa gaaaacagga ttgaggaact tgcaaaacgt
      361 tcggaatatg caaagagtaa tatacttgac atactggcaa aatctgaagg gattgatttt
      421 cctgaaacca taatgaactt tagatggctt gaatcagtac acaactatgc actggcagtt
      481 ggtgcgcggt caatcaagcc cacacttatg gatttcctgc gataataacg ctatgaagag
      541 gtgctaccgt tggtaacaat agcaacaaaa ccatttgggc aaattgaagt tgatgaacgg
      601 cagattattg atttccctga aggcatttat ggttttgagg acattaaaaa atttatcatt
      661 ctggatgcca atgaaaaatc accatttaaa tggctgcagg cgtatgatga acctgattta
      721 gcatttgtga ttatacgccc catagatttt atgatgcagt atgaactgaa cgttatgcca
      781 gaagatttag aggatatagg tgcaaaaagc cctgatgaag tcatagtgtt tgcgatagtt
      841 accataccag aagatccttc aaaaatgacc gccaatctac aggggcctgt ccttattaat
      901 cctaaaacac gacgtggcaa acaggccata tcattaagcg ataaatacac ggtgcgccat
      961 tacatcctgg aagagataaa aaaagcacag gtgaagaggt gattgcatgc tggtactggc
     1021 gcgtaaagta aacgaaagca tcatgattgg cgatgacatt gagatagtta ttattgatat
     1081 caaaggtgat caggttaaat taggaatcaa agcaccaaaa tcggtggcag tgcaccgtaa
     1141 agagatatat gaagaaatac agaaggaaaa tattgccgca atgaaatctc agcttaaacc
     1201 cgaaacactc aaagatatta ctgatatatt gcagactcat aaagaaaaaa acattgacaa
     1261 ccaaaaaaaa taatagtaac acagttcata agtacctgct gtaaatgata acactatcct
     1321 gtcatattca aacatgattc aggacttaca atcacgtgat tgaatccata tcatgttggg
     1381 aatgatggtg gtgaaatcct attttttggg gaagggaaaa tgcccgatat tattgtgcag
     1441 acaaatgttg catatcttgt aggtatattt tttattattt ttattctccg tgagatagtg
     1501 gcctttaaaa agattttgaa gcttaaatat attttcaccc cccttatcac ctatattgtc
     1561 attattattg cattgtatct gtcatatgtc cgtggtttta ccgtgtataa tatctttatt
     1621 attgccggac ttgtctttgc attgattgca gatacttttc ttatgattga agagataagc
     1681 ttttttatcc atggactttt tttcttttta ttaacccatc tgtgctatgt gtatgcgctg
     1741 agtattaatt atcattttgg aaccattgat ataattatta ttatgattat tctatcaatt
     1801 ttctttttgt attatttagt aattcacaaa gcaaaaggta aagtatatat tccggttata
     1861 ctgtatatgc ttgttttatc cttggtattg ctcctggcta ttggtacgtt tctgaaaaat
     1921 gtataccccc ggggtgccat tgtaaccatg gcagcaatcc tttttgccat atctgacggt
     1981 gttcttgcat ttaaccagtt tgtacacaaa gtacctcata gcactgttgt tacatggtca
     2041 ttgtatgcac ctgcacagct tctgtttgca gtctcctgct attattgatg aaaaaaagat
     2101 tattaactca gtgcactatc ttggtggtgt gatgttgtat tgtgtttata tttaaaaaat
     2161 tatcagcaat aatttcttaa aaaatttcat aatgaaaata gtgtacaccc ccgccgccga
     2221 aggcgacggg aatgtacact attttatatt ttattcaaat ttccacattt taggaataat
     2281 tagtgaaaaa ttatcattat ttttcttgta aataatgagt tatttttagt ataatttaat
     2341 gataatactt tgagcatagt gtaaataata tgatggtggt gtgatatgaa aaagatattt
     2401 atttttattc tactaatttt tatttcttgc gggcaattaa taaagcagag cccacaaaag
     2461 gattatgaac gtatatataa aaatttaacc aatgcagaat cactggaaaa tcctgtgcaa
     2521 aaagataagc ttattaacaa agcgtatact cttttgatgc aacggcaggc agaactgata
     2581 aaagaaaaaa aatctgatac aacggtagct gctttactgg cctactatta ttatttaaaa
     2641 gggaactatc aggatgcatc atcacaggtt gaacttgcct cttctccatc cgggaataat
     2701 caattggggg caattataca ggcacgtgta ctgctttcta caaaagggaa agcgggtgct
     2761 cagggtgcac ttgatatctt acgtgcagtg gaaggcttgg ataatccaat ggcatttata
     2821 gcaatgggtg atgcgtattt tttaaaagca gattatgata aggcacgtga taattacaca
     2881 aaagccttat tgcttaacag ggaattgcag gttgttgctg ccaaccgact tgaagttata
     2941 tcccgtataa agacactgtc catcaatgta gcaaaagttt ctgactttat tcttgagccc
     3001 acaatgacaa gagataagct tgcatatata ttatatgaaa tatttgaatt gcaaaagtat
     3061 attgccactg caaagccact tgatgccaat tttattgatc tggaaaagtc tcagtataaa
     3121 agtgcaattg tctctttacg acagaaggga ttcttttcgt atattcaggg gagcgcattt
     3181 gaaccattta tggtagtatc tcgtggtgag atggcaaagc tagttgagga ttttttagta
     3241 ctatcacgta ataatgaagg attacggtca aaatttaaaa atgaatcacc tccttttttc
     3301 aatgatataa aaaatgataa tgaatattat aatgctatgc gtttggctgt tgatatgggt
     3361 attatgactg cttccttgag ccaggatgca tatcctgatg aatcagtaac aggattgcag
     3421 gcaataatga tacttcaaaa attagtaaag taagacacca tttggggatg aggggtatat
     3481 atgattataa aaagaagatt aaagatattt tttacaataa taagtgctct ggttatagca
     3541 gcaataacct atgcgcaatt taccgataat gctcaaagcg atgaaaaaaa ccgcacttct
     3601 aagataacat ataaaaatta tatatggata gaaggtgaaa acgcggtatc aacgaatttt
     3661 gcccgtgaac ccatatataa cttcttttgc agtaataagt ttgcgttaca actttcaaaa
     3721 gatgttgatc ccccacagga cggttatttt gctacctatg ttttttatgt tacccatacc
     3781 aaggaatatg atttctggat gggatgcacc cctcccggat cagcatataa ggataggcct
     3841 ggctatgcat cccccattga atggcaaatt gacaacggcc cgtttcagac agcatcagct
     3901 gagaatgtgt atgtgaaaag tttttatggt attggtgggt tctactgggt aaaaattgcc
     3961 agtggtacac tcaatgctgg gaaacacacc ctgacaatta gagtaaaaca aaaaagaagc
     4021 agcggctggg attactactt ttatgttgat gccattttat tattgcctca atatagtaca
     4081 ggtatagcac agcttatgga ttttcctgag gttgcaccaa aagattttgc cactccgggt
     4141 gatggcatac tgtttaatgc tcctgaattt tatgaaaaaa aattacgtgc aaatattagt
     4201 gatagagaag cactattttc ccttgtgcaa atttatggtt ggctatatga ttataacaaa
     4261 gcaatatttt tactaaaaaa atatttggaa aaaaatccac gtgatcttga tatgcggcta
     4321 gagcttgctg cattttattc atggtcagat caattagatg cagccatcag tgaatataaa
     4381 aatatcattg ctattgatga aaaaaatatc actgcacgaa agcttctttc agtacttgca
     4441 ggctggaaca atcgctatga tgaagcaata aagtattatc aggaaattat tgccatagat
     4501 ccggaaaatg tggatgcgta tatttcactg ggtacacaat attcgtggaa aggtgaaatg
     4561 aacagagcta tcgccacatt tgaaaaggct gaaactattg cccccagtaa tattgaagtg
     4621 cttgaggttt taggtgataa ttattactgg gcaggaaggg cgtatgatgc catacgacaa
     4681 tattaccgca taatcgatat aaatgaaaag cagatatcag catataaaaa attggctaag
     4741 atatatcttg atttaggtga aagtgcaaaa gcagcaagaa taattaatga tgcaaaacag
     4801 gttgtagatt tatatccgga actatcgggt atatcattag atgttaaaga tgagcttgca
     4861 aaagagcgtc gggcattaat agatagttat aaagaggcac tggtaaaaaa tcctgatgat
     4921 gtcaatttac gcaaagggct tgttgatacg tatatatgga acagaatgta tggtgatgcg
     4981 gttaaggaat atgatgctct gcttgcatat aagatgtttc actcaattga gcaggccgaa
     5041 caaaaggtaa cacgattggc catacatact gttggattgc aatcattaat gcctttgatt
     5101 aaaaccatta aggatgatgg tagcataatt aaaaaacgat atgcaaaact gatagatgat
     5161 aaaaagaaag gcaaggctgt cattgagcca tcgtcgcttg ccagcgataa agcaacgctt
     5221 gaatggtaca atgcaaaatt aaaagaaata cagaaagagc tgtatgtata caatgatatc
     5281 actgcggcat ttggcaaaga tgtgcagacg tatacagcaa tgcaggcggc aacaggctgg
     5341 cactttgagt ctgacaggat ttataggtat gctgcaactg caaagaaatt aaatccacaa
     5401 atgtatcaac catataaggt tcagggtacc attgatttat tgtatgataa accgtcaaat
     5461 gcagtcagtt cttttgagtt ggtaaaaaaa ttggctgccg gcgctgaatt tcccgggttg
     5521 ccccttgcct atgcacaaac aaaaaaatac aatgaggcga tagctctttt agaagagtca
     5581 ttgcagagta aaaaagtatc tgcaacatat aagaatgata ttgaacaaat gctatcactg
     5641 gtaaaattaa gtaaaggaga agtatcttct caaagctcac ttcaggtaac tactcaaaaa
     5701 aagtaggcaa ttaaaaaaaa agactattaa ttttaatgga
//
LOCUS       DAOSOX010000151         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14261_length_5713_cov_7.448922, whole genome shotgun sequence.
ACCESSION   DAOSOX010000151 DAOSOX010000000
VERSION     DAOSOX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14261_length_5713_cov_7.448922"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLX22_09740"
     CDS             complement(<1..587)
                     /locus_tag="PLX22_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOT20218.1"
                     /translation="MVQDLKMMYGRLKRWSNQSNCYYENHTRLITIKKILKVAIVIHI
                     AFVVLMGSIIMYYDKHNPSNTPLMYYRAYINNYTVKPVQCVPLKKIPDDIKKMVISIE
                     DYKFYKHNGIDIEAIKRALIINYKVGYRMYGASTITQQLARTLFLLPVKSMIRKYAEL
                     IIALEMEVLLDKDRIFELYLNYCEWGKGLFGIKNAS"
     gene            complement(538..>1395)
                     /locus_tag="PLX22_09745"
     CDS             complement(538..>1395)
                     /locus_tag="PLX22_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOT20219.1"
                     /translation="LNAPFNGRAGHTCTVFKGKLWLIGGWDGNNPYNDVWYSKEGLHW
                     YSAGEMPFKKRAGHTAFVFNDKLYVVGGLSFDECKNIIDMNDVWVSDDGMNWQCIKEM
                     AEFMPRGGHACALFKNKVYLVGGIACGAEAYKTEDGISWNRLASKLPFGSRGGHVLTN
                     FKNKLWLIGGIRVDRNNNFMSFSDVWNSGDGIHWKKVASDMPFFAGGGHGCVVYKNSL
                     WVIGGFRKGGTVYRSDDGMNWELFNSFTPMGERVSHSCTVFKDRIIIIGGFDGTGFKN
                     DVWAFKTLE"
     assembly_gap    1396..1495
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1496..3073)
                     /locus_tag="PLX22_09750"
     CDS             complement(1496..3073)
                     /locus_tag="PLX22_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT20220.1"
                     /translation="MDGTWILTIVTILLPLAYHYDTIKIVYGTIMATILEKYYHVLIA
                     DDEKGIRDLLKDLLEDSFYVNTIDRGDLIIDELKRKKYDILILDLQMPGKNGIEVLQA
                     IHNLKIDIVVVVITASNDVQLAITSMKLGAYDYLVKPFDTDKLLVILKNIVEKLDLEN
                     EVNELREKVGESFRFKNIIGKSPKMQRIFATLERVIDTDSTILIIGESGTGKEIVAKA
                     IHYNSNRKQYPFKSIDCSTIPQDLIGSELFGHEKGAFTGAIARKIGKFEIASKGTLFL
                     DEISNLSFDMQAKLLRVLQEKEFERIGGNEIIKVETRIVAASNRDIREMVKQGTFRED
                     LYYRLNVMPIYLPPLKERKEDIPLFIDYFLKRFTNEYGRTITLDLQSRLYLTDYHWPG
                     NVRQLENVIKRLVLLSTEDVVRLDIVKSVLEFEEYNKPAVQQNVYSSIPSTIATTPVH
                     ESFDNGDTVSDNDTTLQDETVKTLDEVEKEYIEKMLKQFNFNISVTAKAINVSRKTMH
                     NKLKKYNIAIKKTVADD"
     gene            complement(3012..3650)
                     /locus_tag="PLX22_09755"
     CDS             complement(3012..3650)
                     /locus_tag="PLX22_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOT20221.1"
                     /translation="MKTYTMYEVDIIEKIIRNIAHEVRNPLTTIRGYAQLLTQKLDTA
                     SIQKSQKMIIEQAERIDGMFNDLYNAFTIKEDIIVGCLIPDILQKALDASVYHDYIII
                     DDCESFQVQCYMNRLLLCFNTFVNGFNWKYFPDARLRIYVKKETFCTIYFQYTNVIFD
                     ASLENLFFYPFQTKHFFVKGTELFSIYCIANRSGWNMDFNYSNNTFAISIPL"
     gene            complement(3628..4026)
                     /gene="rsfS"
                     /locus_tag="PLX22_09760"
     CDS             complement(3628..4026)
                     /gene="rsfS"
                     /locus_tag="PLX22_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT20222.1"
                     /translation="MTGKSKKSKKQNIDEGTVTLLKGCGKVLDDKKALDIVVLDLRNV
                     NSYLDFFVICTGNSIMHCKALARELTKYFLDSGMKERSKTTANSPWIILDYNEIIIHI
                     FTAEARAFYQLEHLWADASKIDLDENIHDV"
     gene            complement(4023..5144)
                     /locus_tag="PLX22_09765"
     CDS             complement(4023..5144)
                     /locus_tag="PLX22_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOT20223.1"
                     /translation="MGYIKVKDKIRLIVSVTLVCIVILSIFYIYRRFTRNAIQMLAAN
                     KELINILVAGSNDYNERRHSFYAIVHFNQEKNSIGVTFIPPNYLVTLNKRTGKAMRLN
                     MIAFSDSNDIIDSLEDDLHITIPFYLELYGSDVVKIIDLIEGVQLYILDQVKRESGFM
                     RGVHYCDGKKTLWYINKVEHDSIYIKYDRIMDVVLTLFTNKESYKKYNRKSYISEMLS
                     SLNTNLLIQEMLSLADLVYDAKTVYTTIMPGKVDSNDNYTVDDITRKLYEQKFLRPLV
                     LAVSDTESIKVRILNGSDIPGLARKMRNVLTKEDLTVVEFGTSPYSVIDKTVIINQKG
                     NLNSALRVSSILGIDAMYHIVDSSQLADVLVIAGKDYSQ"
     gene            complement(5119..5706)
                     /gene="nadD"
                     /locus_tag="PLX22_09770"
     CDS             complement(5119..5706)
                     /gene="nadD"
                     /locus_tag="PLX22_09770"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT20224.1"
                     /translation="MKVGLFGGTFNPIHIGHLINAQFIREEFNLDFILFIPTKYPVHK
                     ELEYDVDADHRYHMIKLAIEGVEYFKVSRLEIDREKPSYTIYTIDDILKENPEWQLFF
                     IIGYDAFNEIDTWKDWQRLLLTIDFIIMKRPGDILHKRFHQYMPHIHCANNPVIDVSS
                     STIRERVKNGKPIKYLLPYNVEEYIDKHGLYQSEG"
BASE COUNT         1789 a         1123 c          776 g         1925 t
ORIGIN      
        1 gatgcatttt ttatgccaaa taaacctttt ccccattcgc aataattaag atacaattca
       61 aaaattctgt ctttatctaa caatacttcc atctctaaag ctataattaa ttcagcatat
      121 tttctgatca ttgactttac tggtaataaa aacaacgttc gtgccaactg ttgtgtaatg
      181 gtactggctc catacatcct ataaccaact ttataattaa tgatgagagc tcgtttaatt
      241 gcttctatat caataccatt atgtttataa aatttataat cttcaattga gattaccatt
      301 ttctttatat catcaggtat tttttttaag ggtacacatt gcactggttt cactgtataa
      361 ttgtttatat atgcccgata gtacatcaat ggagtatttg aaggattatg cttgtcataa
      421 tacataatta ttgatcccat caacacaaca aaggcaatat gaataactat cgccactttt
      481 aagatttttt taatagtaat taaacgtgta tgattttcgt aataacagtt actttgatta
      541 ctccagcgtt ttaaacgccc atacatcatt tttaaatcct gtaccatcaa aaccacctat
      601 tataattatc ctgtctttga aaactgtaca actatgtgaa acgcgttccc ccatgggagt
      661 aaagctgtta aataattccc aattcattcc atcatcagag cgatacaccg tacccccttt
      721 tctaaaacca ccgattaccc ataaagaatt tttataaaca acacagccat gaccgccacc
      781 agcaaaaaag ggcatgtcgc ttgctacctt tttccagtga attccatcac ctgaattcca
      841 tacatcacta aagctcataa aattattgtt tctgtcaaca cgtattccac caatcagcca
      901 caatttgttt ttgaaatttg ttaaaacatg tccccctcgg gaaccaaaag gtagtttgct
      961 tgcaagcctg ttccagctta taccatcttc ggttttatat gcttcagcac cacacgctat
     1021 gccacctact aaatagactt tatttttaaa taaagcacag gcatgcccac ctcgcggcat
     1081 aaactcagcc atttctttta tacactgcca gttcattcca tcatcactaa cccacacatc
     1141 attcatatca ataatatttt tacattcatc aaatgaaagc ccacctacca catacagctt
     1201 atcattaaaa acaaatgcag tgtgtccggc ccgcttttta aaaggcatct ctccagcact
     1261 ataccagtga agaccctctt ttgaatacca tacatcgttg tatggattat taccatccca
     1321 gccgccaata agccataatt tacctttaaa tactgtacag gtgtgtccgg cacgtccatt
     1381 aaaaggagca ttaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaat
     1501 catcagctac agtctttttt atcgctatat tatatttttt caatttatta tgcattgttt
     1561 ttctggaaac attaatagct tttgccgtta ccgatatgtt gaagttaaac tgctttaaca
     1621 ttttctcaat atattccttt tctacttcat caagtgtttt taccgtttca tcctgcaagg
     1681 tagtatcgtt atcgctaaca gtatcgccat tatcaaaact ttcatgaaca ggcgtcgttg
     1741 ctatagtaga gggtatagaa gaatatacat tttgttgtac tgccggtttg ttatattctt
     1801 caaactccag cacactttta acaatatcca gcctcacaac atcctctgtt gaaagcaata
     1861 caagacgctt gatgacattt tccagttgtc gcacattgcc aggccagtgg taatcggtta
     1921 aatataatcg cgattgtaaa tcaagagtaa ttgttctacc atattcattg gtaaatcgtt
     1981 ttaaaaaata atcaatgaaa agaggaatat cctctttgcg ttcttttaac ggtgggagat
     2041 aaataggcat aacattaagc cggtagtaca aatcttctct gaatgttccc tgtttaacca
     2101 tctcccggat atcccggtta cttgctgcta cgatacgtgt ctccaccttg attatctcat
     2161 tgccaccaat acgctcaaat tccttttcct gcagtacacg cagcaacttt gcctgcatat
     2221 caaacgaaag attgctaatc tcatccagaa atagggttcc cttgcttgca atttcaaatt
     2281 tgccaatttt tcgggcgata gctccggtaa atgcaccttt ttcatgacca aataattcac
     2341 tgccaatcaa atcctgcggt atggtgctgc aatcgataga cttaaaggga tactgtttcc
     2401 ggttgctgtt ataatgaatt gcctttgcaa caatttcttt gccagtacca ctctcaccaa
     2461 tgattaagat agtactatca gtgtcgataa cccgctcaag agtggcaaag atacgctgca
     2521 ttttaggtga tttaccaatt atatttttaa acctgaagct ttcaccaacc ttctcacgta
     2581 attcatttac ttcattttca agatcaagct tttcaacgat attctttaat attactaaaa
     2641 gtttgtcagt atcaaacggt tttaccagat aatcataagc ccctaatttc atagaagtaa
     2701 tagctaactg cacatcattt gaagcagtta tgactaccac aacaatatca atttttaaat
     2761 tatgtatagc ctgtaaaact tcaataccat ttttaccagg catctgcaga tcaagaatta
     2821 aaatatcata tttttttctt ttcagttcat ctatgattaa atcaccacgg tcaattgtat
     2881 tgacataaaa tgaatcttct aataaatcct ttaataaatc tctgattcct ttttcatcat
     2941 cagcaatcag cacatggtaa tatttttcta atattgttgc cataatagtt ccataaacaa
     3001 ttttaatagt atcataatgg tatgctaatg gcaaaagtat tgttactata gttaaaatcc
     3061 atgttccatc cacttctgtt tgcaatgcaa tatatagaaa acaattccgt tccttttaca
     3121 aagaaatgtt tagtttgaaa tggataaaaa aataaatttt ctaaactggc atcaaagatt
     3181 acattcgtat attgaaaata aatggtacag aaagtttctt tttttacata tattcttaat
     3241 ctggcatcag gaaaatattt ccagttaaaa ccattgacaa aggtgttaaa acatagtaat
     3301 aatcggttca tgtaacactg tacctgaaat gattcacaat cgtctattat aatataatca
     3361 tggtatacag acgcatcaag ggctttttgt aaaatatcag gtatcaagca gccaacaatg
     3421 atatcttctt taatggtaaa tgcgttatac aggtcattaa acattccatc aatacgctca
     3481 gcctgttcaa taatcatttt ttgtgatttc tgaattgatg cagtatccaa tttttgagtt
     3541 aaaagttgag catatcctct tatggtagtt aatgggttac gaacctcatg agcaatattt
     3601 cgtataattt tttctattat atcaacttca tacatcgtgt atgttttcat ctaaatcaat
     3661 ttttgatgca tcagcccata gatgctcaag ctgataaaag gcacgtgctt cagctgtaaa
     3721 aatatgtata ataatctcat tataatcaag gataatccat ggcgaattag ctgttgtttt
     3781 tgaacgttcc ttcatgcctg aatctaaaaa atacttagtg agctcacgtg ccaatgcttt
     3841 acagtgcatt atagaatttc ccgtacagat tacaaagaaa tctaaatacg aattaacatt
     3901 tcttaaatct agaactacta tgtccagcgc cttcttatca tccagcactt tgccgcatcc
     3961 ttttagcaag gttactgtac cttcatcaat attttgcttt ttgctttttt tactctttcc
     4021 tgtcactgtg aataatcctt tcctgctatt actaatacat ctgctaattg cgatgaatca
     4081 actatatgat acatagcgtc aatacccaat atagatgaaa ctctcaaagc agaatttaaa
     4141 tttcctttct ggtttataat gactgttttg tcaatgactg aataaggaga agtcccaaac
     4201 tcaaccacgg ttaaatcttc ctttgttaat acatttctca tcttccgggc aagtcctggg
     4261 atatctgaac catttagtat ccgtacctta attgattctg tatcgctaac agcaagaacc
     4321 aaaggccgta aaaatttttg ttcgtacaat ttcctggtaa tatcgtcaac tgtataattg
     4381 tcattactat ctaccttccc tggcattatt gttgtataga cagtttttgc atcatataca
     4441 agatctgcca atgataacat ttcttgtata agtagattgg tattcagtga actaagcatc
     4501 tcgctaatat aactttttct attatatttt ttatacgatt ctttattggt aaatagcgta
     4561 agcacaacat ccattattct gtcatattta atatatatgg aatcatgctc aactttatta
     4621 atataccata gcgttttttt gccatcacaa taatgaacac cacgcataaa tcctgactca
     4681 cgtttaacct gatcaagtat atatagctgc actccctcaa taagatcaat gattttaaca
     4741 acatcagatc catataactc cagatagaag gggattgtta tatgtaaatc atcttctaat
     4801 gaatcaatga tatcattgct atctgaaaac gcgatcatat taagccgcat ggctttgcct
     4861 gtccgtttat tgagtgttac aaggtagttg ggaggtataa atgttacacc aatactgttc
     4921 ttttcttgat taaaatgaac tatcgcataa aaggaatggc gtcgttcatt ataatcattg
     4981 cttccagcaa ctaaaatatt aatcaactct ttgtttgcgg ccagcatttg aattgcatta
     5041 cgcgtaaatc tgcggtaaat ataaaatata cttaaaatta ctatgcaaac cagagttaca
     5101 gaaactatta accgtatttt atccttcact ttgatataac ccatgtttat caatatattc
     5161 ttcaacatta tatggcaaca aatattttat cggcttacca tttttcaccc gttcacgaat
     5221 agttgacgag ctaacatcaa taacaggatt attggcacag tgtatatggg gcatgtattg
     5281 atgaaatcgt ttgtgcaaaa tatcgcccgg gcgtttcata atgataaaat caattgttaa
     5341 aagtaaacgc tgccaatctt tccatgtatc aatctcatta aacgcatcat atcctataat
     5401 aaaaaataac tgccattcag gattttcttt taaaatatcg tcaatagtat atatggtata
     5461 cgatggcttt tccctgtcaa tctcaagacg ggaaacctta aaatactcaa caccttcaat
     5521 agcaagttta atcatatggt atcggtgatc ggcatctaca tcatattcca attctttatg
     5581 tacgggatat tttgtaggga taaatagtat aaaatctaaa ttgaattcct ctcttataaa
     5641 ttgagcatta atcaaatgtc caatatgtat tggattaaat gttccaccaa ataaaccaac
     5701 cttcaatagt ttt
//
LOCUS       DAOSOX010000152         5576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14697_length_5576_cov_8.578156, whole genome shotgun sequence.
ACCESSION   DAOSOX010000152 DAOSOX010000000
VERSION     DAOSOX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_5576_cov_8.578156"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..375
                     /locus_tag="PLX22_09775"
     CDS             238..375
                     /locus_tag="PLX22_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20225.1"
                     /translation="MPQISLYIDKDTHAKIEKAAAKERISISKWIGKNIKKVIKEDYP
                     Q"
     gene            complement(881..1147)
                     /gene="rpsU"
                     /locus_tag="PLX22_09780"
     CDS             complement(881..1147)
                     /gene="rpsU"
                     /locus_tag="PLX22_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT20226.1"
                     /translation="MPVPLEVEVGDGDLERAFKNLKKRMAFEGIFKELKRRRYYEKPS
                     EEKKRKKEEAERRRLKKVRRFETQSKQRRFVAKPSGRGGSSQDE"
     gene            1396..1884
                     /locus_tag="PLX22_09785"
     CDS             1396..1884
                     /locus_tag="PLX22_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20227.1"
                     /translation="MKNCKITILIALFLFVSLYCCSKPVQKSLIPSQPQSPDFNITTI
                     VLECSQKHSNLLPFVELVLLLHNDNTSRYFIATVYGTMYATYTTPPSFAPSHAVCGVT
                     VNHNTLCHYFFVCVTNDAVIIQSTEKKCFDEPDSEKYHTIITIPWKHVNTIQTKVIIN
                     DR"
     gene            1998..2936
                     /locus_tag="PLX22_09790"
     CDS             1998..2936
                     /locus_tag="PLX22_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT20228.1"
                     /translation="MIIGLDIGGTNIKGVIINNDGTILAKTSVITGAGIDATENNIGN
                     CIKTLLELSHKNKNEVDAVGIGSAGAIDKQNGMVITSPNIPAWKHYPIVQRVEKLTGY
                     TVFLENDANAAVVGELWLGNGKNYKNWIMLTLGTGIGGGAVINGMPYTGNNGSAMEFG
                     HITIDYNGLQCKCGRKGCLECYGSATAVVHFVKSHCTFYPDSSLNTISPHAMTARHVY
                     DEAKKGDPLALYVFNLIGTYLGIGIADLVNIFNPEAVILGGGLSSASQYFLPQIIHQV
                     QKLSLEGMKENIRYMITKHGDYTAAIGAAKHALETI"
     gene            2949..3848
                     /locus_tag="PLX22_09795"
     CDS             2949..3848
                     /locus_tag="PLX22_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOT20229.1"
                     /translation="MFFSNQLLFSEHTLNQIHIYAGRWLDFLMVAFTHCGDEIFYILL
                     IPFLFWCVNKRLATIVGLSFLFSTVFNDIIKFAFNKPRPNPLNLAEGIAQLNNVYCPP
                     SSPGFPSGHAQNAVVFWGLLAYTIRRRLFSIFAVFMIVAISYSRLYLGVHFFGDVAGG
                     IIIGCMVFTCAVLLIPYFDRLWNLQLLSAIIIFVMMPVAVAIAFPDDATVKSLGVFSG
                     FSIGLIVTKNRFGEPSFKLVPALVNYFIGITVLLLLKEGIKVILPSVAYADFIRYWII
                     GGWISFGAPLTFNHIPYLSKIYR"
     gene            4092..5129
                     /gene="pheS"
                     /locus_tag="PLX22_09800"
     CDS             4092..5129
                     /gene="pheS"
                     /locus_tag="PLX22_09800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT20230.1"
                     /translation="MYMKQQLENLYKQTIATIEKAVSIDELEQIRVKVLGRKGELTQI
                     LRSLGTLPQEERIETGKLSNELKDSIEEALEKRKTQLTQQHINKLIMEDWLDVTLTKP
                     GLLQEYQRGHLHPISQIQYEIEDIFTSMGFEILDGPEVETDYYNFEALNIPPYHPARD
                     MQDTFWTEDGNLLRTHTSAIQIRGMERLKPPFRVIGPGRVFRYESIDASHENTFYQVE
                     GMMIDKDISVAHLIYFMKTLLKEIFKRDITIRLRPGYFPFVEPGFELDIQCLICGGKG
                     CSVCKYSGWLELLPCGLVQPNVLRYGKIDPDKWSGFAFGLGLNRLVMMRYGINDIRHF
                     LSGDIRFLYQF"
     gene            5164..>5576
                     /locus_tag="PLX22_09805"
     CDS             5164..>5576
                     /locus_tag="PLX22_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT20231.1"
                     /translation="MWISLNIIQDMVDIKDIPVSEIANRLTMSTAEIDSIEHVNQLLN
                     TIYTAKILEVSKHPNADKLTVCKVDTGSNVIQVVCGAPNHKAGDIVALAPAGTKFSDT
                     ITITKTKLRGVESEGMLCSERELGLSDDHSGIMIFP"
BASE COUNT         1672 a         1059 c         1033 g         1812 t
ORIGIN      
        1 gctgcggtga ctatccggat atgatttttt ggaatatact tttgaacagt actcgcatgt
       61 aacatataac atcaaccccc ctatctattt tttggtcata ttattttata tattacaata
      121 ttacaatatt ttttttcata ttagcgacaa atatattggt gcatgaaaaa atattactat
      181 agctaatata cttaactttt atctattatc ctattatcgt tgtcataggg gtttcctatg
      241 ccacaaatat cattatatat cgacaaagat actcatgcga agatagaaaa ggctgcagcc
      301 aaagaacgaa tttcaatctc gaaatggata ggtaaaaata ttaaaaaggt cataaaagaa
      361 gattatccac aatgatattt tgatttattc ggttcaataa cagataaact ggattgtgaa
      421 ataaatgcaa cagccgaaaa caacgcttat gcgcatcgtc gctgcgcttc tcggcctccg
      481 cgccaaaaat atgaagacgg gcgctacggc acattttcac ctccgttatc gctacggtga
      541 aaacgtcata tacgctggat acgttattat gcaagcagca tgttaaactc tttactcacc
      601 tttgaaaaaa ctgctgtttc tgtaaagttt ttgtcattac tgcaaatgca gaaatctaat
      661 acatcaaaat tagatttgtt tgtcattatg tacatataat atttattcat tatttgttga
      721 ctttatccat ccgcctatca tgtcgcacgc ttcattaatt tttaacggat atatgctcac
      781 actattttat ggaaaggtac cacgtatgat aaagactcaa taaataaaaa atccggctga
      841 atgccggatt ttcctgttgt tttcattatc acagttaaaa ctattcgtcc tgtgatgaac
      901 cacccctgcc agaaggtttt gcaacaaacc tgcgttgctt tgattgagtt tcgaatcttc
      961 tgactttttt caggcgccgc cgttctgctt cttccttttt gcgcttcttt tcttcgcttg
     1021 gcttttcgta ataacgcctg cgtttaagct ctttaaaaat cccttcaaat gccattcgtt
     1081 ttttcagatt tttaaatgca cgctcaagat cgccatctcc tacctcaact tctaaaggta
     1141 ctggcatatt gtaatcacat cccttcttaa ttgttttaca ttattttaat gtaattatta
     1201 tattgtagct ctatcacgtt tacattactg aaaatactgt acttttgtca acataaaaaa
     1261 ttatatctct ccttttgcca ttagttttaa caaatattca tgaaaacggt tgacaataca
     1321 taaaaatatg ctctttaata gtacaccatg cataataagg aatttgatac tttgttttat
     1381 ttaatagctg tatatgtgaa aaattgtaag attactatat taatagcatt atttttattt
     1441 gtatcgttat actgttgcag taaacctgtt cagaagtcac tcatcccatc acaaccgcaa
     1501 tcgcctgatt ttaacataac cactattgtt ttagaatgct cgcaaaaaca tagcaatttg
     1561 ttgccttttg tggaattggt acttttgctg cacaatgaca acaccagtcg ttattttatt
     1621 gcaacagtat atggaactat gtatgccact tatactaccc ctccatcctt tgcaccttca
     1681 catgcagtat gtggagttac cgtaaaccat aacacactgt gccactattt ttttgtatgc
     1741 gtaaccaacg atgctgttat tattcaatca acagaaaaaa aatgttttga tgaacctgat
     1801 agtgaaaagt atcatactat tatcaccata ccatggaagc atgtaaacac catacaaact
     1861 aaggttatta taaatgacag gtaatgaaat aaattttcaa tggttattgc catgaataat
     1921 aaaaaattat tgattacctg ctggttgcaa tcattatatg gctatctttc aataaatagt
     1981 aatgcaggaa ttttttcatg attattggac tggacattgg cggaacaaat atcaaaggcg
     2041 tcatcatcaa caatgatggc actatactgg caaaaacatc agtcataaca ggagcaggta
     2101 ttgatgccac tgaaaataat attggtaact gtatcaaaac attactggaa ctatcgcaca
     2161 aaaataaaaa cgaggttgat gctgtaggca ttggttctgc cggtgctatt gataaacaaa
     2221 acggtatggt aatcaccagc ccaaatattc ctgcatggaa gcactatcca attgtacagc
     2281 gtgtggaaaa gcttaccggt tatacagtgt tccttgaaaa tgatgccaat gctgcagtag
     2341 ttggcgagct atggcttggc aacggcaaaa attataaaaa ctggattatg cttacccttg
     2401 gcaccggcat agggggcggt gctgttatca atggtatgcc ctataccggc aataacggga
     2461 gcgctatgga atttggacac ataactattg attataacgg cctgcaatgc aaatgcggca
     2521 gaaagggatg tctggaatgt tacggctcgg caacagcggt tgttcatttt gttaagagtc
     2581 attgtacatt ctatcctgat tcttcactca ataccatatc accacatgcg atgaccgcac
     2641 gccatgtcta tgatgaagca aaaaaaggtg atccgcttgc tctgtatgtt tttaatctca
     2701 ttggaacata tttgggaatt ggcattgctg atctggtaaa tatatttaat cctgaagcag
     2761 tgatactggg cggaggatta tccagcgcca gccagtactt tcttccgcaa attattcatc
     2821 aggttcaaaa gctttccctt gaagggatga aggaaaacat acgctatatg attaccaaac
     2881 atggagatta tacagcagcc attggcgcag caaaacatgc attagaaact atataacagg
     2941 aggatgttat gtttttcagc aatcagcttt tattcagcga acatacgctc aatcagattc
     3001 atatctatgc tggcagatgg cttgattttt taatggttgc ctttacccat tgcggcgatg
     3061 agatatttta catcctgtta ataccgtttc ttttctggtg tgtcaacaaa cggcttgcta
     3121 caatagttgg attgtcattt ttgttttcca ctgtttttaa cgatattatt aaatttgctt
     3181 ttaataagcc gcggccaaat cctttgaact tagccgaagg tattgcccag cttaacaatg
     3241 tatactgccc tccatcaagt cctggatttc caagcggaca tgcacaaaac gcagtggttt
     3301 tctggggttt gctggcatat acaatcaggc gcaggttatt ttccatcttt gcagttttca
     3361 tgatagttgc aatttcgtat tcacggcttt accttggggt tcattttttt ggcgatgtag
     3421 cgggtggtat catcatcggc tgcatggtgt ttacatgcgc cgtgctgctt atcccctatt
     3481 ttgacagatt gtggaatcta caattactat ctgccattat tatttttgtc atgatgcctg
     3541 ttgcagtagc cattgcattc cccgatgacg caacggttaa atcgctcggt gttttttcag
     3601 gattttctat cggcttaatt gtcactaaaa atcgttttgg cgaaccatca tttaaacttg
     3661 tgcctgcact ggttaactat ttcattggga taaccgtatt gcttctttta aaagagggca
     3721 tcaaggtcat tttaccttct gtggcatatg cagattttat tcgctactgg ataataggcg
     3781 gctggatttc gtttggtgcg ccattaactt tcaatcatat cccctattta tctaaaattt
     3841 acaggtaatg ttgttgactt tttataaaca gttgatttgt aatacacttc taaaatttat
     3901 tttgacagta actatcaccc atactcattt ttttggcgat agttactcgt tatgtatttt
     3961 gttttccggg cattgttgaa aattgttttt ttattgtaat attaatggag caggcgaatc
     4021 taacaccact gcatgctcat agtagtcaca atgaaatctt caggtgccca ttattattta
     4081 tgtgtcaggt gatatatatg aaacaacaac tggaaaacct gtataaacaa accattgcta
     4141 ccattgaaaa agctgtctcg atagatgagc tggaacaaat acgcgttaag gtattgggac
     4201 gcaaaggcga attgacacag attttgcgtt cgctgggtac actaccacag gaagagcgga
     4261 ttgaaacagg aaaactttca aatgaattaa aagatagcat tgaagaagct ctggaaaaac
     4321 gtaaaacaca actaacgcaa caacacatta ataaactcat catggaagac tggcttgatg
     4381 taacgctgac aaaaccaggt ttattacagg aatatcagcg cgggcacttg catcctatat
     4441 cacagataca atatgaaatt gaagatatct ttacctcaat gggttttgaa attcttgatg
     4501 gtccggaggt ggaaaccgat tactataact ttgaagcttt gaatattccg ccctatcatc
     4561 cagcccgtga tatgcaagac acattctgga cggaagatgg taatttactg cgaacgcata
     4621 catcggctat ccaaatacga gggatggaac gccttaaacc gccattcaga gttatagggc
     4681 ctggacgcgt tttcaggtat gaaagcatcg atgcatcaca cgaaaacaca ttttaccagg
     4741 ttgaaggcat gatgattgat aaagatattt ctgtggctca tttaatatac ttcatgaaaa
     4801 cattgctcaa agaaatattt aaacgtgata ttaccatacg gttgcgcccc ggttatttcc
     4861 cttttgttga accgggtttt gaacttgata tacagtgtct tatttgcggc ggcaagggtt
     4921 gcagtgtatg caaatattca ggatggcttg agcttttacc ctgcggactt gttcaaccca
     4981 atgttctgcg ctatggtaaa attgatcctg ataaatggtc gggatttgcc tttgggttgg
     5041 gcttaaaccg tcttgttatg atgcggtatg gcattaatga tatacgtcac tttttaagtg
     5101 gcgatatacg tttcctctat caattttaat gatataattt tataattgta cggagtcgtt
     5161 actatgtgga tttcacttaa cattattcag gacatggttg atataaagga tattcctgtt
     5221 agcgaaatag ctaatcgttt aacaatgtct actgctgaaa tagattctat tgaacatgtg
     5281 aatcaacttt taaacactat ttataccgcc aaaattctgg aagtatcaaa acatcccaat
     5341 gccgataaac taaccgtttg taaagttgat accggcagca atgttataca ggttgtgtgc
     5401 ggtgcaccaa atcataaagc aggcgatatt gttgcattag caccggcagg gacaaagttt
     5461 tccgatacaa tcaccataac aaaaacaaaa ttgcgcggtg tagaatccga agggatgtta
     5521 tgctccgaac gtgagcttgg cttatcggac gatcattctg gtataatgat ttttcc
//
LOCUS       DAOSOX010000153         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14748_length_5560_cov_8.383106, whole genome shotgun sequence.
ACCESSION   DAOSOX010000153 DAOSOX010000000
VERSION     DAOSOX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14748_length_5560_cov_8.383106"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLX22_09810"
     CDS             <1..237
                     /locus_tag="PLX22_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20232.1"
                     /translation="SITMNYLTEFFQNEMKLTQKEKDELESKLKHSKHIKEKPKKPAA
                     DKHNAMTNHVVESWTKLDKAKWKKEKKKHKPYSE"
     gene            complement(225..1118)
                     /locus_tag="PLX22_09815"
     CDS             complement(225..1118)
                     /locus_tag="PLX22_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20233.1"
                     /translation="MRHNNCYYCGINGFIFKSYRFEDMAIIADIKIEKKHEGWPGIPH
                     GGLSMAAFVELIDIAKESAALYPHEYHFRFGGERLSCGDAVRCIVQKGDEKNFFGIFG
                     RSLQNPYCHCRYSETRVHPYNNDVIDVLKQNMDAATPFIIPNMSVNLINNKPGNNNSR
                     MFYITDYQLDRSYLIARLHAPNNFVPMDDSSIHQGVLCSLLDETMGWSTFLSVWQGGV
                     TVELKITFFDKVYNSDDVYVIGFCDTVKGEYGKKIASAYGGVFAQKDDDMEIIALAHG
                     RWLTLAGFKEIMLNYLKHYSE"
     gene            1588..2448
                     /locus_tag="PLX22_09820"
     CDS             1588..2448
                     /locus_tag="PLX22_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20234.1"
                     /translation="MVLILVTVLISTTGIAIDFSFQPIVIENLSRYNEDRIAYQHVQK
                     DVSPNLDNSFSALLIIKSKKVYLIQDGYDNPENVSTKKLQFEMENKPLDDPWENKINN
                     KPDYVRITERKIELLKNFGEDFVSKNFGNFFLSVRNALIKKHVEVFKTLMVDRKESGL
                     VVTRTPLPVPAYLNAPETPTKYKMTVSGKTIDEKLYYAEDIDGDGITETFTVNSPDGF
                     NWGYKSGPNIILILNNKDEEIKGLIGQLCNWAYYGTPEEEKEILHNFPTDMDIINEFK
                     LEAVPTQQTK"
     gene            complement(2495..3394)
                     /locus_tag="PLX22_09825"
     CDS             complement(2495..3394)
                     /locus_tag="PLX22_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT20235.1"
                     /translation="MTYRYIDLHIHSQYSEDGDYSVDHIFQMAKTIGLSAISITDHDA
                     IDAIPEGIVCSEKYNIEFIPGIELTTIYEDGSQQHILGYFINNAPVLNAIVKKIGNYR
                     HEIAKKRIQKLRDIGFVMNEKNVWRKANNRPPTATAIMLEVFENPHNRNDKRLEEYFN
                     GKKSDNKLMYFYREYLAEHMPAYVPFISISTHEGIEVVKATGGVPVLAHPKFVKGEEH
                     LKKIAVMGIAGIEAISSYHTKEDIDYFIRFAADHNLIITAGSDFHGPTSKPKVTLGGI
                     QGNDYRLLDELKKYRERSKTVIQ"
     gene            complement(3414..4136)
                     /locus_tag="PLX22_09830"
     CDS             complement(3414..4136)
                     /locus_tag="PLX22_09830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT20236.1"
                     /translation="MNIQKCVIVFLLVIVSFSFAGELYFIDNENIQRYNGKSGKWIEV
                     NMVKQIKALANQFGITVEDVYCINNIKEKQFKRSFVFFPYSDIYIQQLMEKGISLQPL
                     NVPYDAFIWPIGDPEKISSGLGRRWGRFHPGVDIPAPKGTMVRATMEGKVIYAGYFGN
                     YGNSIVLEHRDNYVTRYAHNSVLLVKVGDYVQKGQAIAFVGSTGRSTGNHLHFEIRCA
                     DIPLNPLDLLPEKSLIIMKNPK"
     gene            complement(4388..5209)
                     /locus_tag="PLX22_09835"
     CDS             complement(4388..5209)
                     /locus_tag="PLX22_09835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT20237.1"
                     /translation="MKLDNIVIAVSISSVMIVLAYLLFYYKTDAYVINNPVDIKHEEN
                     INITRLHKVPIILYHNIDGKGTYSIELKKLKNQFDLLKKHLIKVISLQTLESHINGHN
                     PFNQKVVSITFDDGYRSMYTKLMPLADSLNYPFTLFVYTDNIYYKSKNNLTWEMVKEL
                     HDHNIDIQCHSQSHPDLVKLYNKRTMQSKYELYREIYLSKKILELYLQKPIYYFAFPY
                     GRYTIELTELCRDAGYKRVFSTDYGPNIVIHDNFCLRRHHIKSNYRNEKVLTIAW"
     gene            complement(5196..>5560)
                     /locus_tag="PLX22_09840"
     CDS             complement(5196..>5560)
                     /locus_tag="PLX22_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT20238.1"
                     /translation="SAYGTSKFAVFGLADCLRGEFKEKGICLSVLCPPEVNTPFIAEE
                     LKTIPKKTKRIKKMTGMLSAETIAKKIVSAIQKNKYMIVPGTMSKITWLTHRMFNGYT
                     TRIITDGIIKFLGNDETR"
BASE COUNT         1867 a         1032 c          832 g         1829 t
ORIGIN      
        1 tcaatcacca tgaactatct aacagaattt tttcaaaatg aaatgaagct aacgcaaaaa
       61 gaaaaggatg agctggaaag taaactaaaa catagtaagc atattaaaga aaaaccaaaa
      121 aaacctgctg cggataaaca caatgccatg acaaaccatg tggttgaaag ctggacaaaa
      181 cttgataaag caaaatggaa aaaggaaaag aaaaaacata aaccctattc agaataatgc
      241 ttgagatagt ttagcattat ctctttaaat cctgccagtg taagccatcg cccatgtgca
      301 agcgcgatta tttccatatc atcatccttt tgtgcaaaaa caccaccata tgcagaagca
      361 atctttttgc catattcacc cttcactgta tcacaaaaac caatgacata tacatcatcg
      421 ctgttataaa ctttatcaaa aaaggtaatc ttaagttcaa cagtaactcc gccctgccac
      481 accgatagga atgttgacca gcccattgtt tcatcaagca atgagcataa caccccttga
      541 tgaatagaac tatcatccat tggtacaaaa ttattagggg catgtaaccg tgcaataaga
      601 taacttctat caagttgata gtcagtaata taaaacatcc ttgaattatt atttcctggt
      661 ttattattaa taagatttac tgacatgttg gggataataa atggtgttgc ggcatccatg
      721 ttttgtttta atacatcaat gacatcattg ttataaggat gcacacgagt ttcactatac
      781 cggcaatggc aatagggatt ttgcaacgat ctgccaaaaa taccaaaaaa atttttttca
      841 tcgccctttt gaacaatgca acgcacagca tccccgcatg ataaacgttc accaccaaat
      901 ctgaaatgat attcgtgggg atagagtgca gctgattctt ttgcaatatc aatgagttca
      961 acaaaggctg ccattgacaa tccgccatga ggaatccccg gccagccttc atgttttttc
     1021 tctatcttga tatcagcaat gatggccata tcctcaaaac ggtacgactt aaaaataaag
     1081 ccattgatac cacaataata acaattatta tgtctcatat tttttcttgt aatagaacaa
     1141 tcaatcccta tataatagtt tatcccctat attctacagt tatgcaatta tctcaatgaa
     1201 tgaaaaaaaa taacaataaa gcataaataa tgctatacaa tacaggttgc aaactatata
     1261 ttaaaaatca aaacaatttt tttttaaact ataaaatatt tgacaaaaat attttgttac
     1321 aataatctat gcttgtattg ttgaattata attgaaataa tgcattttta aatcccggac
     1381 agtacactat tacgggtttg catgtaacaa aaattgatta cgtatgggca ccataataaa
     1441 tcaggctttt catatttaaa aaataattaa acctgatttt ttcaatgatt gtgcccgcag
     1501 gggaacatcc cgagtataat tattagaggt tcccttataa tgaatagatg aatggaggca
     1561 tatatgtgca tattcaggaa aaaaaatttg gtacttatac tagtcaccgt tttaatctca
     1621 acgacaggta tcgcaattga tttttcattc cagccaatag ttatagaaaa cttatcacgg
     1681 tataatgaag acaggattgc atatcaacat gttcaaaaag atgtatcccc taatttagat
     1741 aattcatttt cagccctttt aataataaaa tcaaaaaagg tttatcttat ccaggacggc
     1801 tatgataatc ctgagaatgt cagtaccaaa aaattacaat ttgaaatgga aaacaaacct
     1861 cttgatgatc catgggaaaa taagataaat aataaacctg attatgtacg tataacagag
     1921 cgcaagatag aattattgaa aaattttggt gaagattttg tatcaaaaaa ttttggcaat
     1981 ttctttttaa gtgtacgcaa tgcattgata aaaaaacatg ttgaagtatt taaaacactt
     2041 atggtcgacc gcaaagaatc agggcttgta gtcacccgaa cacctcttcc agtacctgca
     2101 tatcttaatg cacccgaaac gccaacaaaa tataaaatga ccgtttcagg aaaaactatt
     2161 gatgaaaaac tatattacgc agaggatatc gatggagatg gaataactga aacttttacg
     2221 gtcaatagcc ctgatggttt taactggggc tataaatccg gccccaacat tatcctcatt
     2281 ttaaataata aagatgaaga aattaaaggt ttaattggtc agctatgtaa ctgggcatac
     2341 tatgggaccc cagaagaaga aaaagaaata ttgcataatt tccctactga tatggatatt
     2401 attaatgaat tcaagctcga agcagtacca acacaacaaa caaaataata attgattcat
     2461 ttatttatag tagaggtagg tttataacct gcctctactg aattactgtt ttgcttcttt
     2521 ctctgtactt ttttaactcg tccagcagcc tataatcatt cccctgtata ccacctaatg
     2581 taacctttgg cttagaggtt gggccatgaa aatccgaacc tgctgtaata atcagattat
     2641 ggtcggcagc aaatcgaatg aaataatcaa tgtcttcttt tgtatgatac gacgatatag
     2701 cctctatacc agctatcccc attactgcaa tctttttcag atgttcttcc cctttaacaa
     2761 attttgggtg agcaagcaca ggtacaccgc ctgtagcttt aaccacttca attccttcgt
     2821 gcgtgctaat tgatataaat ggaacataag caggcatatg ttcagccaga tattctctgt
     2881 aaaaatacat taatttgtta tctgattttt tcccattaaa atactcttca aggcgtttat
     2941 catttctatt atgtggattt tcaaatactt caagcataat ggctgttgcc gtaggaggcc
     3001 gattattagc cttcctccat acattttttt cattcattac aaagccaata tcccgcaatt
     3061 tttgaatacg cttttttgct atttcatggc gatagttacc tatttttttt actatcgcgt
     3121 tgagcacagg ggcattgtta ataaaatagc ccagtatatg ctgctgggaa ccatcttcat
     3181 aaattgtagt taactcaatt cctggaataa actctatgtt atatttttct gaacaaacaa
     3241 ttccctcggg aattgcatca attgcatcgt gatcagtaat actgatagca cttaatccaa
     3301 tcgtttttgc catctgaaaa atatgatcta cagaataatc accatcttca ctatactgcg
     3361 aatggatatg caagtctata tatcgatagg tcataactgg agcatagcac acattatttg
     3421 gggtttttca ttattattaa actcttttca ggtaacaagt ccagtggatt aagaggaata
     3481 tcggcgcatc taatctcaaa atggaggtga ttacctgttg aacgtcctgt actgcctaca
     3541 aaagctatcg cctgaccttt ctgaacataa tcgcctacct ttacaagcaa tactgaatta
     3601 tgggcatagc gggtaacata attatcccta tgttccagaa caatggaatt gccataattg
     3661 ccaaaatatc ctgcataaat tactttacct tccattgtag ccctgaccat cgtgcccttt
     3721 ggcgcaggta tatcaacccc cggatgaaac cgcccccatc ttcttcctaa tccagaacta
     3781 attttttcag gatcaccaat tggccatata aacgcatcat agggaacatt taacggttgt
     3841 aacgaaatcc ccttttccat taactgctgg atatagatat cagaataggg aaaaaacaca
     3901 aaagatcttt taaattgttt ttctttaata ttgttaatac aatacacatc ttcaacagtt
     3961 ataccaaact ggttagccaa agcctttatc tgcttaacca tgttcacttc aatccattta
     4021 cctgattttc cattataacg ctgtatattt tcattatcta tgaagtaaag ctcacccgca
     4081 aatgaaaaag aaacaataac aagaagaaat actataacac acttttgtat attcataaca
     4141 gtacaatcct gtacaaaaaa atttaaaaca acatatactc cctatcttat taataaaacg
     4201 aagtattcta tttatcgtat taatatagtt atttataagt acacagtaag cttattgtat
     4261 aatttttaat cactactttt tgtcaacaaa aatttcatcc tttaccatat gtcaactgct
     4321 ttgtgagcgt attcttagtc ctctatcttt acctgcttga tttttcccac agtttatgag
     4381 tagttaccta ccatgcaata gtcagtactt tttcattacg atagttactt ttgatatggt
     4441 gtcttcgcaa acaaaagtta tcatgtatta caatatttgg tccgtaatca gttgaaaata
     4501 cacgtttata cccagcatca cggcatagtt ctgtaagctc aattgtataa cgaccgtatg
     4561 ggaaagcaaa gtaatatatt ggtttttgca aataaagttc taatattttt ttagataaat
     4621 atatttcccg gtacagttca tatttactct gcattgttct tttgttataa agttttacaa
     4681 gatcaggatg actttgcgaa tggcattgta tatctatatt atggtcatgt aattctttca
     4741 ccatctccca tgttaaatta tttttgcttt tataataaat attgtcagta tacacaaaaa
     4801 gcgtaaaggg gtagttcaga ctatcagcca atggcatcaa tttagtatac atcgacctat
     4861 atccatcatc aaatgttata gatacaacct tctgattaaa tggattatgt ccattaatat
     4921 gcgattccaa tgtttgtagt gaaattactt tgatcaaatg tttttttaac agatcaaact
     4981 gattttttag tttttttagc tcaatagaat atgtaccttt gccatcaatg ttatgataca
     5041 atataatagg tactttgtgt aatctggtaa tattgatatt ttcttcatgt tttatatcta
     5101 ctgggttatt tattacgtat gcatcagttt tatagtaaaa taacaggtaa gccagtacta
     5161 tcataacact ggaaatgctc actgctatta caatattatc tagtttcatc attccccaaa
     5221 aattttatta taccatcagt tataattctg gttgtatatc cgttaaacat tctatgggta
     5281 agccatgtta ttttgctcat cgtgcccgga actatcatat acttattttt ttgtattgca
     5341 cttactattt ttttagcgat agtttctgct gaaagcatcc ctgtcatttt tttaatgcgt
     5401 ttggtttttt tagggattgt tttaagctct tcagcaataa acggtgtatt aacttcgggt
     5461 gggcatagta ctgaaagaca tattcctttt tctttaaact cacctcgcaa acaatcagcc
     5521 agtccaaata ctgcaaattt tgatgtccca tacgctgagt
//
LOCUS       DAOSOX010000154         5530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14828_length_5530_cov_7.213151, whole genome shotgun sequence.
ACCESSION   DAOSOX010000154 DAOSOX010000000
VERSION     DAOSOX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14828_length_5530_cov_7.213151"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1025
                     /locus_tag="PLX22_09845"
     CDS             267..1025
                     /locus_tag="PLX22_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20239.1"
                     /translation="MYVGKEQSGDLGVCYSSVQGDAIDGKHVGIGFVGLGNIWGPYIG
                     IAIGTYCDFSWGNLYDNDKLDYINFNIRIPVSLVIQVVRIDNAAINIYGGGDINGKST
                     WFDLPTVTTGNSYDTYKLTSLAGSFPLGYHVGLQASGRYAGLAITALAGMQSIYFKES
                     KYEYENTDDDNTYYFKIPAETIKNYFYGIKLSVIPWDISLSYTYQKFDKSDKTGFPEF
                     SQHTFMITFLVAGKHADVKEEKKEEKPKQQVDAV"
     gene            1089..2069
                     /locus_tag="PLX22_09850"
     CDS             1089..2069
                     /locus_tag="PLX22_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOT20240.1"
                     /translation="MNSAKSFVNMIAISSLLIIGGCVSQQEVKPVKKFPFPEPIVYNI
                     AFCKPVNVYTKEKADAILQNLQPYIYQLRSEDVLAKTDDVIVDNEAIKINWNWEELQT
                     ETLSQKSTTSQENDVTGDVIKGILSGKKEDMIGALISGAANSSSQTTTTNMQVTKKVE
                     KHNKTIIMFEDVSNIFRFMSNYIIIRLKSGEDVYIICNNEYYLQKWIDAFMSAMKARG
                     YNTDFTLGVMCQKQPMNAEQIDYLQVNSGAVVLGIMPGSPAAEMGLEYLDVITKVNGM
                     AISSGEELTAYIKSKKPKNNERLTFEIVKSKREGEEVSKVVIRKDLIVKN"
     gene            complement(2066..3622)
                     /locus_tag="PLX22_09855"
     CDS             complement(2066..3622)
                     /locus_tag="PLX22_09855"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOT20241.1"
                     /translation="MNIKLLAMQNNRVTITILPENRSVTVPAGTTIADALLNCNIILN
                     TPCGGKGICKKCTVHASGLLSQPDDYEKSLGDSSLRLACRAVIIGDATIWIDSITQRP
                     RYPELNPTNTYAVAIDIGTTTVQIAFFDMTTKQATPEITLLNPQRRFGLDVMTRLSHA
                     NDPAKASHLTQILLQTIITTIQDVCNISGIDIHSIVTIAISGNTVMSYSFLGIDITPL
                     GTYPYTTLITSFDNYNHYPPLKKTFPNAHITVLPVVSAFLGGDFLGGVGTLPKTKGNS
                     FFFDIGTNGEMAIIKEDGTIVATSCAMGPALEGMNISSGMTATEGAITHFNLYNNTLT
                     YTSLGDGKPSGISGTALIDLIAILLKNGVIEKSGAIKTAIPQPLSKHIQLEHIENMKT
                     LIILDIITINQQDIRNVQLAKGASLAAAKILLREAGLSAHDIEQVYIAGAFGQHINID
                     NFNELSFLPEFKQATYHCAGNTSLKAAITACTDNEFLQRLLQRKEHITTIDLSQHHEF
                     NDIYIKSLDF"
     gene            3760..4893
                     /locus_tag="PLX22_09860"
     CDS             3760..4893
                     /locus_tag="PLX22_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20242.1"
                     /translation="MDATIISYPDTSVVQLLSLTENRSKYMLPFAGKFRVVDFVIGNA
                     KEVDAHTIIYSNVNDDLADYVKLHGIDESRLKVVVDNFNSVDFFYSIVRDSDSAYFII
                     YNGDNPGIINFSHLIERFITIKKPSVLYKMHFEEKARMAHRILVTTKKALLDTIRKAL
                     KHNHSAPNIFEMIINMMVNIGLAKGEFSCMYWPFSTIVDYYNHNFDVINNADYSKHVF
                     DGHINAKLELYRYSMLGPDARISRSFLSDSTYINGEVVNSIIFPGVEIGKDAVIHDSI
                     ILPYNKIGSKARIMRTIIDERTVEPFSADIINIGNNCKIGTNEIGMKNSDYPRVLYNS
                     ITFIGKNCIIPDGITVGGACYIASGRAQQDFAKRKYIYNGTSV"
     gene            4877..>5530
                     /locus_tag="PLX22_09865"
     CDS             4877..>5530
                     /locus_tag="PLX22_09865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT20243.1"
                     /translation="MVPRCDMFFPYSDENPTRSFPLFTVILILANCVIYYMEVYGQPA
                     FAQLINSYALVPCNVNKLHLDDYRYYVPFISHMFLHVNIIHVAGNMLFLWIFGNNVED
                     VLGGIRFYAFYVLCGLAAALTFYLFHHTECTPIIGASGAVSGVLGGYVVLFPSARVKV
                     WFIFFVLRLRAWFFILLWFLLQLSNLYGEQSGVSNVAWSAHVGGFIAGFVLIMFFKKR
                     "
BASE COUNT         1677 a          866 c         1172 g         1815 t
ORIGIN      
        1 gaatatacac acattctacg tgaagggttt agggtttttt tcttaatagc tgaatagtta
       61 ctaatattta aggtttaagg gaaatattta atcatcatca attttatctg gaggatagtg
      121 tcatgaggaa atcaatgctg agtattatgg tgctaatttt tatgatgtcg gcttcaaaag
      181 tttttgcaac tgatattaat attggtactg ttccacttcc caatttatat attgcagcac
      241 cgccaattca attaccatgg tttgaaatgt atgttggaaa agaacaatca ggtgacttgg
      301 gtgtttgtta tagtagtgta cagggtgatg cgattgatgg caagcatgta ggaataggat
      361 ttgtcgggtt agggaatatt tgggggcctt atatagggat agcaattgga acctattgcg
      421 atttcagctg gggaaatttg tatgataatg ataagcttga ttacataaat ttcaatatta
      481 gaatacctgt gtcactagta atccaggttg tcaggataga taacgctgca atcaatatat
      541 atggcggagg tgatattaat ggcaaatcta cttggtttga tttgcccact gtaactactg
      601 gtaactctta tgacacttat aagctaacat ctttagcagg ttcgttccca ttagggtacc
      661 atgtagggct tcaggcaagc ggtaggtatg caggactagc tattacggca cttgcaggga
      721 tgcaaagtat atatttcaaa gaaagtaaat atgaatatga aaacacagac gatgataata
      781 cctactattt caaaatacct gctgaaacaa taaaaaatta tttttatggt ataaagttaa
      841 gtgtaatccc atgggatatt tcattaagtt acacctatca aaaatttgat aagtcggata
      901 agacaggatt tcctgaattc tcacaacata catttatgat tacattttta gttgctggaa
      961 aacatgctga tgttaaggaa gagaaaaagg aggaaaaacc aaaacagcag gttgatgctg
     1021 tataaattta aaggttgtaa actatggtgg tacttttttt tatgtcaatt taacttaatg
     1081 aggttaatat gaattctgct aaatcatttg tgaatatgat tgccatatct tcgttattaa
     1141 taatcggtgg ctgtgtatca cagcaggaag ttaaacctgt aaaaaaattt ccgtttcctg
     1201 aaccaatagt atacaacata gctttttgca aaccagttaa tgtttatact aaagagaagg
     1261 cagatgccat tttacaaaat ttgcagcctt acatttatca gttgcgtagt gaagatgttt
     1321 tggcaaaaac tgatgatgta attgtggata acgaagcaat aaagataaat tggaattggg
     1381 aagaactgca aactgaaacc ttatcccaga aatcaacaac ttcacaggaa aatgatgtta
     1441 ccggtgatgt cataaaaggg atattaagcg gaaaaaaaga ggatatgatt ggagccttga
     1501 tatcaggagc tgcaaattca tcatcgcaaa ctaccacaac caatatgcag gttacgaaaa
     1561 aagttgaaaa gcataataaa acaatcatta tgtttgaaga tgtgagtaat atattcagat
     1621 ttatgtctaa ttacattatt attagattaa aaagtggtga ggatgtttat ataatatgta
     1681 ataatgaata ttatctgcag aaatggattg atgcttttat gtctgcaatg aaagcacgcg
     1741 gttacaatac tgattttaca ctgggtgtaa tgtgtcagaa gcaaccaatg aatgctgagc
     1801 aaatagatta cttgcaggtc aatagcggtg cagtggtatt gggcattatg ccaggcagcc
     1861 ccgcagcaga aatgggtctg gaatatcttg atgttataac aaaagtaaat gggatggcaa
     1921 tatcatcggg tgaagagtta acagcgtata taaaaagcaa aaagcccaaa aataatgaac
     1981 ggttgacatt tgagattgta aaatcaaaaa gagaaggaga agaggtatca aaagttgtta
     2041 ttagaaagga tctgattgta aagaattaaa aatccagtga tttgatatat atatcattaa
     2101 attcatgatg ctgtgataaa tcaatggttg taatatgttc ttttctttgt agtaaccttt
     2161 gtaaaaattc attatctgtg catgcagtaa ttgctgcttt taggcttgtg ttccctgcac
     2221 aatgataggt tgcttgttta aattcaggca gaaaggataa ttcattaaaa ttatcaatat
     2281 ttatatgttg cccaaatgca ccagctatat acacctgctc aatatcgtga gctgacagtc
     2341 ctgcttcacg taataaaatt tttgcggctg caaggcttgc tccttttgca agctgaacat
     2401 tgcgaatatc ctgttgattg atggtaatga tatcaagaat gatcagggtt ttcatgtttt
     2461 caatgtgttc aagttgaatg tgctttgata gcggttgtgg tatagcagtc tttatggcac
     2521 cacttttttc aataacacca ttttttagaa gaatagcaat gaggtcaata agtgcagtgc
     2581 cgcttatccc tgatggtttg ccatcaccta acgaagtgta ggtcaacgta ttattgtaaa
     2641 gattaaagtg ggtgatagct ccctcggttg cagtcattcc acttgaaata ttcattccct
     2701 ccagagctgg ccccatagca catgatgttg caacaatagt gccatcttct tttataattg
     2761 ccatctcacc gtttgtgcca atgtcaaaga aaaagctgtt gccttttgtt tttggaagtg
     2821 taccaacacc acctaaaaag tcaccaccca gaaatgcact gactactggt aaaacggtta
     2881 tgtgtgcatt gggaaatgtt tttttcaaag ggggataatg gttatagttg tcaaaagacg
     2941 tgatgagtgt ggtataggga taagtaccta aaggggtaat atcaatccct aagaaggaat
     3001 aactcatcac agtgttgcca ctgatggcga tagttactat ggagtgtata tcaatacctg
     3061 aaatgttgca tacatcctga atagtggtta ttattgtttg taaaagaatc tgtgtgaggt
     3121 gactggcttt agcagggtca tttgcatggc ttaatcgcgt cataacatcc agcccaaatc
     3181 gtcgttgtgg attaaggaga gtgatttcgg gcgttgcctg tttggttgtc atatcaaaaa
     3241 aagctatctg cactgtggtt gtgccaatgt caattgctac ggcgtaggtg ttggttgggt
     3301 taagctctgg atagcgaggg cgctgggtaa tgctgtcaat ccagatagtt gcatcaccta
     3361 taattacagc acggcaggct aatctcaagg aactatcgcc caaacttttt tcataatcat
     3421 caggttggga tagcaaacct gaagcgtgta ctgtacattt tttgcatatt ccttttccgc
     3481 cgcatggtgt gttgagtatg atattgcaat taagcaatgc atcggctatg gtagtgccag
     3541 cgggtactgt tacactccgg ttttcaggga ggatggtaat tgttacacga ttgttttgca
     3601 ttgctaataa ctttatattc aattatagca acatatactt gctattattg aaaagtaaat
     3661 attttattat ttttttcttt acagatgaaa tgtccttgct attgaattgc cctgttgttt
     3721 taatcgtcat gaacttttgt tataggatgc actgtgcaca tggatgcaac aattatttca
     3781 tatcctgata cctcggttgt acaattactt tcgcttaccg aaaacagatc aaaatacatg
     3841 ctgccatttg ctggtaaatt tagagtggtt gattttgtca ttggcaatgc aaaagaggtt
     3901 gatgcacata ccatcattta cagcaatgtc aatgatgatc tggccgatta tgtaaagctt
     3961 catggtattg atgaatcaag gctcaaggtt gtggttgata attttaacag tgttgatttt
     4021 ttttattcaa ttgttcgtga tagcgattca gcatatttca taatatataa tggtgataat
     4081 cccggtatta taaatttttc gcatcttatt gaacgtttca ttaccataaa aaaaccttcg
     4141 gtactgtata agatgcattt tgaagaaaag gcaaggatgg cgcatcgcat ccttgttacc
     4201 actaaaaagg ctttgcttga taccatacga aaagcattaa agcataatca tagcgctccc
     4261 aacatctttg aaatgattat taatatgatg gttaatatag ggcttgcgaa aggtgaattt
     4321 tcatgtatgt actggccctt cagcacaatc gttgattatt ataatcacaa ttttgatgtt
     4381 atcaataatg ctgattattc aaaacatgtg tttgatgggc acataaacgc aaagcttgag
     4441 ctataccgct attccatgct tggtccagat gcaaggatat cacgttcatt tttgtcagat
     4501 agtacctata taaatggcga ggtggttaat tctatcatat tcccgggtgt tgaaattggg
     4561 aaggatgctg ttatccatga ttccattatt ttgccctata acaagattgg atcaaaggcg
     4621 cgtatcatgc gtacaatcat tgatgaacgt accgtagagc cttttagtgc cgatataata
     4681 aacataggga ataactgcaa gattggtaca aacgagatag ggatgaaaaa cagtgattat
     4741 cccagggtgc tgtataacag cattaccttt attggtaaaa attgcataat ccctgacggt
     4801 ataacggtgg gcggggcatg ctatattgca tcaggcaggg cacagcagga ctttgcaaaa
     4861 cgaaaatata tctataatgg tacctcggtg tgatatgttt ttcccttatt ctgatgaaaa
     4921 tcccactcgc tcatttccac tttttactgt tattcttata cttgcaaatt gtgtcatcta
     4981 ttatatggag gtttatgggc aacctgcatt tgcacaactc atcaacagct atgcgcttgt
     5041 cccctgtaat gtgaacaagt tacatcttga tgactatcgc tattatgttc cttttataag
     5101 tcatatgttt ttgcatgtta atattattca tgtggcaggc aacatgcttt ttttgtggat
     5161 atttggtaat aatgttgagg atgtgctggg aggcatacgc ttctatgctt tttatgtatt
     5221 atgcgggctt gctgctgctt taacatttta tttatttcat catactgaat gtacaccaat
     5281 cataggagca tcaggtgccg tgtcgggggt actgggtggg tatgtggtac tgttcccatc
     5341 ggcgcgtgtc aaggtgtggt ttatattttt tgtgctgcga ttgcgtgcat ggttttttat
     5401 actgttgtgg tttttactac agttgagcaa cctttacggt gagcagtctg gagtaagtaa
     5461 tgtggcatgg tctgctcatg ttggtggatt tattgctggg tttgtgctca ttatgttttt
     5521 taagaagaga
//
LOCUS       DAOSOX010000155         5515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_14873_length_5515_cov_7.944322, whole genome shotgun sequence.
ACCESSION   DAOSOX010000155 DAOSOX010000000
VERSION     DAOSOX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14873_length_5515_cov_7.944322"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLX22_09870"
     CDS             complement(<1..233)
                     /locus_tag="PLX22_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20244.1"
                     /translation="MRTLLQHINSLNSKERTIIASNLGFHNPMKASVKDIYSRMASYM
                     GIELIFSQCSAEEIAILKLLSVSDLHYRDIEHTL"
     gene            366..1436
                     /gene="lepB"
                     /locus_tag="PLX22_09875"
     CDS             366..1436
                     /gene="lepB"
                     /locus_tag="PLX22_09875"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT20245.1"
                     /translation="MLQYLALNKQNTDTIQINLPDNIFIIRLMLKRIGYLILLIYSTI
                     FLAVAIPFEATYAIGGFLCCIFLLRIINDFIKWKKFGNVSISISPSGITVTTKNETYH
                     FNAKDITYLEVNPFSNFVIRERHRSLSLPLMLLHKEDREKLLNYFLDMSPKRTVMFKK
                     IWEMFDAILVAFILAMHIRQFIIQAYYIPTGSMEDTLLVGDHLMAEKITYGPTIPRMI
                     GMEAPVHLSFLSIRGVQRGDIIIFRPPNEEDKDFIKRCIAVEGDDVHIEDGAVWINGV
                     KLDEPYVKGTTSYRGFSEKRIEGIVPKGMIVAMGDNRENSYDSRGFGYVSLDRIKGKA
                     FILYWNTDNIKRLDFSRFGLIK"
     gene            1452..3275
                     /locus_tag="PLX22_09880"
     CDS             1452..3275
                     /locus_tag="PLX22_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT20246.1"
                     /translation="MENISHKRRILLTLVFVSFIAIVLIFNVTRLHFSTKVIVQNKND
                     TIKRGDIVDVNGYPLALSIYKYSIYADPRKVDNPEEVAQKLSSVVALSYNELKDLLTR
                     DKHFVWIKRKIDDAVFQQVQQLSLHGIYYKKEYARVYPEGSMAAQVLGFVNIDNIGLE
                     GIEYKYNDILLSGDNSQNEENKGLTVQLTLDREIQYIAQKTLNAYSEQVMPQRAIALV
                     QEIKTGKILGLATYPQFNPETFYNYDRSILTCFSVVDSFEPGSTMKLFAAVTLLEHLP
                     DALKKTFICKGSIDVFDATIKCTKEHGRVNLNDIITYSCNAGIIQAMQYVKKDDFYQT
                     LVRFGFGEKTGIQLSGESSGILRPPKTWSGLSKFSMAIGQELSVTSVQLTSAIAAIAN
                     KGIYMYPQIVNQIENTNGNVVSAFFPRSKGKILNEHIAQSILLMMRNVVIEGTGKKAS
                     VAGYETGGKTGTAQKSEHQGGYIPNVYVVSFIGIAPLENPDICVLILFDEPHRGGSGG
                     ELAAPVFSDIVKRILPLRGFGRGTVKQQSPSPVSYTKKKIMHVPNFIGYKDAEALREL
                     INIQQNYPIQYIMKGSGTVVSQDPQPGLPLTKVTTISLTME"
     gene            3278..5128
                     /locus_tag="PLX22_09885"
     CDS             3278..5128
                     /locus_tag="PLX22_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOT20247.1"
                     /translation="MENYFQKNISAIVTINPLVANTINKARDNLSIEVVQSKTGKPIP
                     IIKKEHTITVHSRFDPEKEADRLIEEFDCVHYDCIIVSGFGFGYHIEKILQKLKPDAT
                     LLILERFAWMVKTAMCNRDLQSIFHDKRLILLINPSDDDIAVTMKGRSSYKTLFITHR
                     GSFQIDPEGYTNLQRIAKAYLATKEVNIATLAKFEKLWTSNIAKNIKTIIAHPPASAF
                     FNKFTTIPAIIVNAGPTLTESIPFIKENCNNALIIAVDTTYKVLEYYNIQPHFCITVD
                     PQLVNARYFEGAHENKTILIADPMVHPSTFLFYKGRKAITGVAFEMMQWIETIIGDYG
                     KLRYGGSVSTNAYDFAKRLGANPIVMVGQDLAFTRGLAHVKGAYLDEHVYLRTNRFYT
                     VEMFNRFQLTALPKIFVKGIKSKQVHTNQKMMIFISWFSRLHDSALINATYDGAYIDT
                     IVHKNSADISLSCNNVNIRDIIDTVYTETMPSSEIISHRSLKMIEQLNQMLSQTTVMN
                     TMLQQALRYSDDLINNIKMKNSKAITSLINKLNDIDRSISNLTITKNIVGLTIQHVIH
                     TVMEGYEIDETIKDEQEATALRSKYLYTGLRDGLQFSKRIIENMIMLLSQ"
     gene            complement(5129..>5515)
                     /locus_tag="PLX22_09890"
     CDS             complement(5129..>5515)
                     /locus_tag="PLX22_09890"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT20248.1"
                     /translation="GYGFGRIGCHIAGDGCYGIETSSILGVAYPDGIVPISYTVYPTP
                     LFESFFSFIVAIVLLQLRKKELPQGMLFFIYLIFNGVSRFCVEFIRRNPVVFAGLTQA
                     QIIAFCFVIAGIAGIVFINKQKLKKA"
BASE COUNT         1919 a          897 c         1030 g         1669 t
ORIGIN      
        1 agtgtatgtt caatgtcgcg gtagtgaaga tctgaaacgg atagtaattt taaaattgct
       61 atctcttcag cagaacattg agaaaaaata agttctatcc ccatatagct tgccatgcgg
      121 gaatagatat ctttaactga agccttcatg ggattatgaa agccaaggtt ggaagcaatt
      181 atggtgcgct ctttgctatt taatgaatta atatgttgta atagtgttcg cattatttaa
      241 aaagtagtag acacattgat tgcaataata tacatgatat catcttggct ttacaataaa
      301 aaatgtaact tttatcataa ctatgcatat atatactata tactataaaa atttgaggta
      361 catagatgtt acagtattta gcactcaaca aacaaaatac cgatactatc cagatcaatc
      421 tgcctgataa tatatttatt atcagactta tgttgaaacg gataggatat cttatattat
      481 taatttattc aactattttt ttagctgtag caataccttt tgaagctaca tatgctatcg
      541 gtggatttct atgttgtata tttttgctaa gaataatcaa tgattttatt aaatggaaaa
      601 aatttggaaa cgtttctatt agcatttcgc catcagggat aactgttact acaaaaaatg
      661 aaacgtatca ttttaatgca aaggatataa cgtatttaga agttaatcca tttagtaatt
      721 ttgtaataag agagcgtcac agaagtttaa gtttaccctt aatgctgtta cataaagaag
      781 atcgagaaaa gcttttaaac tattttctgg atatgtcacc taaaagaact gttatgttta
      841 aaaaaatatg ggagatgttt gatgcaatcc ttgtggcatt tattcttgca atgcatatcc
      901 gacaatttat tatacaggcg tattatattc caactggatc tatggaagat accctgcttg
      961 ttggggatca cctcatggca gaaaagatta cttatggccc aactatcccc agaatgattg
     1021 gaatggaagc gccagtacat ctttcatttt tgagtatacg gggagtgcaa agaggtgata
     1081 taataatctt cagaccaccc aatgaagaag ataaagactt cattaaacga tgtattgctg
     1141 tggaaggtga tgatgtacat attgaagatg gtgcggtgtg gattaatggt gttaaattgg
     1201 atgaaccgta tgtaaaagga actacatcat atagaggatt tagtgaaaaa cgcatagagg
     1261 gaatagttcc taaaggaatg atagtagcaa tgggtgacaa cagggaaaat tcatatgata
     1321 gccgtggctt tggatatgtt tcccttgaca ggatcaaagg taaagcattt attttatact
     1381 ggaatacaga caacataaaa cgtcttgatt tttcaagatt cggactaata aaatagagct
     1441 tttaagatga tatggagaac atatcgcata aacgaagaat cctgcttaca ttagtctttg
     1501 taagttttat tgctatagtt ttaattttca atgttacacg gttgcatttc agtacaaaag
     1561 taattgtaca aaataaaaat gatactataa agcgagggga tatagttgat gtaaatggct
     1621 acccccttgc actatcaatc tataaatatt caatttacgc cgatcctcgc aaagtagata
     1681 atcctgaaga agttgcacaa aaactttcat cggttgttgc tctatcctat aacgaattaa
     1741 aagatttatt aacgcgtgat aagcattttg tatggattaa acgtaagata gatgatgcag
     1801 tttttcaaca ggttcaacaa ttatcgttgc atggcatata ctataagaag gaatatgcac
     1861 gagtctatcc agaaggttca atggctgcgc aggtacttgg atttgttaat attgataaca
     1921 tagggcttga gggtattgaa tacaaatata atgatatact attatcgggc gataactcac
     1981 aaaatgaaga aaacaaaggg ttaacagttc aactgacatt agatagagag atacagtata
     2041 tagcgcaaaa aactcttaat gcttattcag agcaggtgat gccccaaaga gctattgctc
     2101 tggtacagga gattaaaaca ggtaagatac tgggattagc cacctacccg caatttaatc
     2161 cggaaacatt ttataattat gatagatcaa ttttaacatg cttttcagta gtggattcat
     2221 ttgaaccagg ttcaacaatg aagctatttg ctgctgtaac attacttgaa catcttcctg
     2281 atgccttgaa aaaaacattt atttgtaaag ggagcattga tgtgtttgat gcaaccatta
     2341 aatgcactaa agaacatggc agggttaatt taaatgatat tattacctac tcatgtaatg
     2401 caggtatcat tcaggcgatg cagtatgtta aaaaggatga tttctatcaa acacttgttc
     2461 gctttggatt tggagaaaaa accggaattc aactttcagg cgaatcgtca ggaatactca
     2521 ggcctcctaa aacatggtca ggtttgtcaa aattttcaat ggcaatagga caggaactgt
     2581 cggtaacttc tgtacagctt acatctgcta tagcagctat cgccaataaa ggaatatata
     2641 tgtatcctca gatagtaaat caaattgaaa atacaaatgg taatgttgta tcagcgtttt
     2701 tccccaggag caaaggaaaa atattgaatg aacatattgc gcagtctatt ttgcttatga
     2761 tgcgtaatgt tgttattgaa ggtaccggga aaaaagcatc tgtagctggc tatgaaacag
     2821 gtggtaaaac gggtacagcg caaaagtcgg aacaccaggg aggctatata cccaatgttt
     2881 atgttgtatc atttataggt attgctccgc ttgaaaatcc cgatatatgt gtactgatat
     2941 tatttgatga accacaccgt ggaggatcag gcggggaatt agcggctcct gttttcagcg
     3001 atattgtaaa gcgaatattg ccattgcgag gttttggcag gggaaccgtt aagcaacaat
     3061 cgccatcacc ggtgtcatat acaaagaaaa aaattatgca tgttcccaat tttattggct
     3121 ataaagatgc tgaagcactg cgcgagctta taaatattca acaaaattat ccgatacaat
     3181 atataatgaa aggttcaggt actgtagttt ctcaggaccc gcaaccggga ttaccattaa
     3241 ctaaagttac caccatatcg ttaaccatgg agtagatgtg gaaaattatt tccaaaaaaa
     3301 tatttcggcg atagttacta ttaatccact ggttgctaat acaataaaca aagcccgtga
     3361 caatctttct atagaagtgg tgcagtctaa aacaggaaaa ccaatcccta taataaaaaa
     3421 agaacacacc ataacagtcc acagcaggtt tgaccctgaa aaagaagctg accgcttaat
     3481 agaagaattt gattgtgttc actatgattg tattattgtt tcagggtttg gatttggata
     3541 ccacattgaa aaaattttac aaaagttaaa acctgatgca acgctgctga tattggaacg
     3601 ttttgcatgg atggttaaaa cagcgatgtg caatcgtgat ttgcagagta tattccatga
     3661 taaaagactt atactattga ttaatccatc tgacgatgat attgcagtaa ctatgaaagg
     3721 cagatcatcc tataaaacct tatttattac acatcgcggt tcgttccaga ttgatcctga
     3781 aggctatacc aatttacaac gaattgcaaa agcttatctg gcaaccaaag aagtaaatat
     3841 tgccacatta gcaaaatttg aaaaactgtg gacctcaaat attgctaaaa atattaagac
     3901 cataattgcg catccgccgg catcagcatt ttttaataaa tttactacca tccctgcaat
     3961 tattgtgaat gctggtccaa ctttgactga aagcataccg tttataaaag aaaattgtaa
     4021 taatgcgttg atcatagcag ttgatacaac gtataaggtt ctagagtact ataatatcca
     4081 gccacatttt tgcatcaccg ttgatcctca gcttgtcaat gcgcgatact ttgaaggagc
     4141 gcatgaaaac aaaaccatac tcattgcaga cccaatggta catccttcaa cctttttatt
     4201 ttacaaggga aggaaagcaa ttaccggtgt tgcatttgag atgatgcaat ggattgaaac
     4261 catcattgga gattatggca agcttagata tggcggatcg gtttcaacca atgcctatga
     4321 ctttgcaaaa aggttaggag ctaatccaat tgtgatggtt ggacaggatt tagcgtttac
     4381 cagagggctt gcccatgtta agggagcata tttagatgag catgtatatt tacgaacaaa
     4441 tcgtttttat actgtcgaga tgtttaaccg atttcaacta accgcattac caaagatctt
     4501 tgttaaaggg attaaaagca aacaggtgca taccaatcaa aagatgatga tttttatatc
     4561 atggttcagc aggttacatg attcagcatt aatcaatgct acgtatgacg gagcttatat
     4621 tgatactatt gttcataaaa attctgctga tataagtttg tcttgtaata atgtcaatat
     4681 tcgggatatc attgacactg tttatactga aacgatgcca tcatcagaga ttatatccca
     4741 tcgttcatta aaaatgattg aacaattaaa tcagatgctt tcacaaacaa cggtgatgaa
     4801 tacaatgttg caacaggcat tacggtattc tgatgactta ataaacaata ttaaaatgaa
     4861 aaattcaaag gctatcacat cacttataaa caaattaaat gacattgata gatcaatttc
     4921 aaacctaact atcaccaaaa atattgttgg acttactatt caacatgtta ttcatacagt
     4981 tatggaaggt tatgagattg atgaaacaat aaaggatgag caggaagcaa cagctcttcg
     5041 ctcaaagtat ttgtatactg gcttgcgtga tggattacaa tttagcaaac gtataattga
     5101 aaatatgatt atgctattat cacaataact aagctttctt taatttttgt ttattaatga
     5161 aaactatacc agcaatacca gcaatcacaa aacaaaatgc aattatttga gcctgggtta
     5221 aaccagcaaa aacaaccgga ttgcggcgga tgaactcaac acaaaagcgt gacacgccat
     5281 tgaaaattaa atagataaaa aaaagcattc cctgagggag ctcttttttc cttagttgaa
     5341 gcaacactat tgcgacaatg aatgaaaaaa aggattcaaa taatggagta gggtacacgg
     5401 tgtatgatat tggtacaatt ccatcagggt atgctactcc taaaatactt gaagtttcta
     5461 ttccataaca accgtcgcca gcaatatgac atcctattct gccaaaacca tagcc
//
LOCUS       DAOSOX010000156         5477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15009_length_5477_cov_7.762449, whole genome shotgun sequence.
ACCESSION   DAOSOX010000156 DAOSOX010000000
VERSION     DAOSOX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15009_length_5477_cov_7.762449"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLX22_09895"
     CDS             complement(<1..824)
                     /locus_tag="PLX22_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT20249.1"
                     /translation="MNYTHYSQVFLDQVNKYGDRVAMRYKPKDKWEEITWNQFGDLVN
                     KTSKALLECGIGEQETVGIFSQNMPLWTVADIASLCIRGIPVPIYATNTAKQAEYIIN
                     DAEIKVLFVGDAEQYSKIMQILPENKHLQKIILFKESVPIQKSDNIMYFKDFLKIGEQ
                     SNKDNMLSERMQRAKIDDLLTLIYTSGTTGEPKGVMLTHSNVIFQKGEHDLRLIDPND
                     NDVSLAFLPLSHVFERIWTYYVFATGMTNNYLEDTGKIIECIQEVKPTIMCAVPRF"
     gene            1238..1726
                     /locus_tag="PLX22_09900"
     CDS             1238..1726
                     /locus_tag="PLX22_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOT20250.1"
                     /translation="MDYVKCLLLENGVGSLLDFTDSKYIKFLKSRNKYKAIEELAGVF
                     KGTDICSDIKAFVAALKEREEIMTTGIGFGIAVPHAKLESIHKIAFAIGISKKGIDFN
                     SIDGRPVHMVVLVAAGERQHKDYLKLLSKIMSLLKNDTIRVEIINAKSAKEIINILRN
                     AK"
     gene            1755..>2377
                     /locus_tag="PLX22_09905"
     CDS             1755..>2377
                     /locus_tag="PLX22_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT20251.1"
                     /translation="MKKLHSLLKKYKCIITTFFTTRYDIREYIVQIGMALCIGAIAGL
                     GAMVFHTLIEWMRIVINPKHFADYLEIPSITIVIIPVIGGLITIGMTKMFPWQAKDRG
                     VKSIIRSVMLQNGHIPIVNTLFHLIASAVTLGTGAPLGPEGPSAKIGGGLGSYISTLL
                     RFNSRKKITYTVAGAGAAISAIFNAPIAGVFFGIEVILLNDLKNESL"
     assembly_gap    2378..2477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2478..3286
                     /locus_tag="PLX22_09910"
     CDS             <2478..3286
                     /locus_tag="PLX22_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOT20252.1"
                     /translation="INVLIMLLVLKLLFVALYIQTGAYGGLFAPSLVIGVLVGFLFAS
                     FFSYLGVKLDPAGYAIIGMGGILAGLNSIPITAILMVFELTNDYRIILPIMLASVISH
                     LTVTFYNKGTVYELELLEDGLDVSKSRINILESIQVLSILKKDFQTINARTPLNQTLD
                     KLLDSDSGLIVVDDNNEYVGILSINEVRQALLSSNIVELLICRDIVIHVEPLQKTDSA
                     AEALKKMDKHGIDFLAVVEKNKVVGMVQHRDIISIYNKLVMQLEEKAMAM"
     gene            3800..>5477
                     /gene="leuS"
                     /locus_tag="PLX22_09915"
     CDS             3800..>5477
                     /gene="leuS"
                     /locus_tag="PLX22_09915"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT20253.1"
                     /translation="MSEAEYNPKKIEKKWHERWDESFIFQSKKDTDRNKYYILEMFPY
                     PSGRIHMGHVRNYSIGDVVARYKRMQGFNVFHPMGWDAFGLPAENAAIKHGIPPKKWT
                     DDNIDYMKKQFKMLGYSYDWNREIATHTQEYYKWNQYIFIKMYEKGLAYKRKAPVNWC
                     PTCNTVLANEQVENGVCWRCETQVTQRELEQWFFKITDYAEDLLAGHEILKEGWPERV
                     IVMQRNWIGRSTGLIVNFKVADTGEDFPIFTTRPDTIYGVTFMVIAPEHPFLERIKDQ
                     KVKDFIKRIKEQSLIERLSEEKEKEGIDTGIKIINPFNGDIVPLYVGNFVLMDYGTGA
                     IMAVPAHDTRDFAFAKKYNIPIKVVIDNPQHPVDSNTMSDAYVDDGICVSSGPFTGMH
                     NRDAIEAIVDYAEKEGIGKRKINYRLKDWLISRQRYWGCPIPIVYCEKCGAQPVDYKD
                     LPVILPVDVDFHGDSRSPLTMMPDFYNATCPVCGGSAQRETDTMDTFVDSSWYFAKFT
                     SPHSTEIFDFDEVKYWTPVDQYIGGIEHACMHLLYARFFTMVLNDLGLLQY"
BASE COUNT         1714 a          812 c         1069 g         1782 t
ORIGIN      
        1 taaaatcgtg gaacggcaca catgatagtt ggttttactt cctgtatgca ttcaataatc
       61 ttcccggtat cttctaagta gttattggtc ataccagtag caaagacata gtatgtccag
      121 atgcgttcaa agacatggct caacggcaaa aaggccagag aaacatcatt gtcattgggg
      181 tcaatcaatc ggagatcatg ctcacctttt tgaaaaatta cattggaatg ggttaacata
      241 acaccttttg gctcacctgt tgttcctgat gtataaatta aggttagcag atcatcaatt
      301 tttgctcttt gcattcgttc tgatagcata ttatctttat ttgattgctc accaattttc
      361 aaaaaatctt tgaaatacat tatattatca gatttttgta ttgggacgct ttctttaaag
      421 agaattattt tttgtaaatg tttgttttca ggaagtattt gcattatttt tgaatattgt
      481 tcagcatcac cgacaaatag tacttttatc tctgcatcgt tgatgatata ttcagcctgt
      541 ttggccgtat tggtggcata aataggcacc ggtatgccac ggatacatag tgaagcaata
      601 tcagctactg tccatagggg catgttttga gaaaaaatac caacggtttc ttgctcacca
      661 ataccacatt caagtaatgc tttacttgtt ttattaacta aatcaccaaa ttgattccat
      721 gttatttctt cccatttatc ctttggctta taacgcatcg caactctatc accgtattta
      781 ttgacctgat ctaagaatac ttgagaataa tgtgtgtaat tcatattgcc actccttttt
      841 atataaagtt ttataaatta ttatttatta ttgttcatta agattttaac atataaaata
      901 tgataaagtc aataattttt tttgattttt ttgaaaactt tcctatttct atatagaata
      961 gtataaacaa atactgtctt tttcaagtgt aataatatga aaagaatagt ttttatagat
     1021 atatttcatc cataagcatt tttaacattg cttgttttaa tatttacctc aaccaatcat
     1081 attataaaaa atcagcactg gtcaacatgc aataattgat aatagtatcc atactaaatt
     1141 atttttattg aaaaatggta gatcatagca tgatgtaagc tcaatatatt tagaattttt
     1201 gtaattaagt aattgacagg gagtatttta ttatgcaatg gattatgtaa aatgtttatt
     1261 gttggagaat ggtgtgggct cattgcttga ttttacagat agtaaatata ttaaattttt
     1321 aaaatccagg aataaataca aggctataga agaacttgca ggagttttta aaggtactga
     1381 tatctgttca gatattaaag catttgttgc tgcactaaaa gaacgtgaag agataatgac
     1441 tactggtata ggatttggta ttgcagtacc tcatgccaaa ctggaatcta ttcataaaat
     1501 tgcatttgct attggtattt ctaaaaaagg gatagatttt aattccattg atggaagacc
     1561 ggttcatatg gtggttctgg tagcagcagg tgaacgtcaa cataaagatt atctgaaatt
     1621 attatcaaag ataatgtcat tacttaaaaa tgatactatt agagtggaaa taataaacgc
     1681 taaaagtgct aaagaaatta ttaatatttt aagaaatgca aaataatgat aaacacgttt
     1741 tataaagccg cacgatgaag aaattgcata gtttgctcaa gaagtataag tgcattatta
     1801 caacattttt cacaacccgt tatgatatac gtgaatatat agtacagata gggatggcgc
     1861 tgtgtatagg tgctattgca ggtttagggg caatggtatt tcatacactt attgaatgga
     1921 tgcgtattgt cattaatccc aagcattttg cagattattt agaaattcct tcaattacta
     1981 tagttattat tccagtcatt ggtggtttga ttacaatagg aatgacaaaa atgttcccat
     2041 ggcaggcaaa agatcgtggt gtaaaaagta tcatacgatc tgtaatgctt caaaacggac
     2101 atattccaat tgttaacaca ttatttcatc tgattgcttc tgctgttaca ttaggtacgg
     2161 gcgccccatt aggtcctgag gggccgtcag ctaaaattgg tgggggtttg ggatcatata
     2221 tttctacttt gctacgattt aatagcagga aaaaaataac atacactgta gcaggtgcag
     2281 gcgctgctat ttcagcaata tttaatgctc ccattgctgg agtttttttt gggattgagg
     2341 ttatattgct caatgatctg aaaaatgaat ccttgagnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnncaa ttaatgtgct tataatgcta ttagtattga aactattatt
     2521 tgttgcatta tatatccaaa caggtgctta tggtggattg tttgcaccat cgttagtaat
     2581 aggtgtgtta gttgggtttc tatttgcttc atttttttca tatttagggg taaaacttga
     2641 tcctgcaggc tatgcaataa ttggtatggg aggtatttta gcaggattaa attcaatccc
     2701 tatcactgct atcttgatgg tatttgagtt gaccaatgac tatcgcatta tccttccaat
     2761 tatgctggct tctgtaattt cacatttaac ggttacattt tataataaag gcacagtcta
     2821 tgaattggag cttttagagg atggactgga tgttagcaaa agccgtatta atatacttga
     2881 atcaatacag gtgttatcga ttttgaaaaa ggacttccag actataaatg caagaacacc
     2941 cttgaatcaa acattggata agcttttaga tagcgattca ggactcattg tcgttgatga
     3001 taacaatgaa tatgttggga ttttatcaat aaatgaggta cgtcaggcgt tgctatcaag
     3061 taatattgtg gaattattaa tatgccggga tatagtaatc catgttgagc cacttcagaa
     3121 aacagattcc gcagcggaag cattaaaaaa gatggataaa catggtatag attttcttgc
     3181 ggtagttgag aaaaacaagg ttgttggaat ggtacagcat agggatataa ttagcattta
     3241 taataaactt gtgatgcagt tagaagaaaa agctatggct atgtaggttt ttcaatatat
     3301 tagtgctcat tatttaagtc atgctgcaaa atacttgacg attacggtca ttttcgtaaa
     3361 atgatactat acattgagca aatttgtttt ttggggtttt tcataaccgc agggtagctg
     3421 tccatagttt ggcaagctca ccaaccacgt cctgtaattg gacgaagtta gcatggagga
     3481 aaacggttcc aaactaattt ttttaatggt ggataagcat ttaccatagg gggaaatggt
     3541 gcccaagatt aagcacaata acctatgaca ataataatat ttttttgaga agttatccat
     3601 ggtctgtctg tgaccaccca tacgggatga taataattgg cggctgtaat cctgaataat
     3661 gccatgaagt ggtttcagga tattgtttaa ttatttggat tccggagcag gtccagaatg
     3721 acaatggggg caaacgtaga tttcagggca caaccataat gacagtagta catacttcta
     3781 ttttcggagg acttaaacaa tgtcagaagc tgaatataat ccaaaaaaga ttgagaagaa
     3841 gtggcatgaa cgatgggatg aatcatttat atttcaatcg aaaaaagata ctgacaggaa
     3901 taagtattat atattagaaa tgtttcctta tccatcaggg agaatacata tgggtcatgt
     3961 gcgaaactac tctattgggg atgttgttgc acgatataaa cgtatgcagg gatttaatgt
     4021 atttcatcct atgggttggg atgcttttgg cctgccagct gaaaatgcag ctatcaaaca
     4081 tggcatacct cctaaaaaat ggactgacga taacattgat tatatgaaga aacagtttaa
     4141 aatgttgggc tattcgtatg attggaacag agaaattgct acccatactc aagaatatta
     4201 taaatggaat caatatatat ttattaagat gtatgagaag ggacttgcct ataaacgtaa
     4261 agctccggtc aattggtgcc ctacctgtaa tacggttctg gcaaatgaac aggttgaaaa
     4321 cggagtatgc tggaggtgtg aaacgcaggt tacacaacgt gagctagagc aatggttttt
     4381 taagattact gattatgctg aagatctatt agcagggcat gaaatattga aggaaggttg
     4441 gcccgaacgc gtaatagtta tgcagcgcaa ctggataggg cgttctactg gacttattgt
     4501 caatttcaag gtagctgata ctggtgagga ttttccaatt tttaccacac gcccagatac
     4561 catctatgga gttaccttta tggtgatagc tcctgagcat ccattccttg aacgaataaa
     4621 agatcagaag gtaaaggatt ttataaagcg gattaaagaa caatcactta ttgaacgcct
     4681 ttcagaagaa aaagaaaaag aaggtataga tactggaatt aaaataatta atccttttaa
     4741 tggagatatt gtgcctctgt atgttggcaa ttttgttctt atggactatg gaacaggtgc
     4801 gattatggca gtacctgctc atgatactcg tgactttgcc tttgctaaaa aatataatat
     4861 ccctattaag gttgtaatag ataatccgca gcatcctgtt gatagcaaca ccatgagcga
     4921 tgcgtatgtt gatgatggaa tatgtgttag ttcaggcccg tttacaggta tgcataatag
     4981 agatgccatc gaagcaatag tcgattatgc agaaaaagaa ggtattggaa aaaggaaaat
     5041 aaattatagg ttaaaagact ggcttatttc tcggcagcga tactggggat gtcctatccc
     5101 aattgtatat tgtgaaaagt gtggagccca gcccgttgac tataaggatt tacctgttat
     5161 tttaccagta gacgttgact tccacggaga ttctcgttca ccacttacca tgatgcctga
     5221 cttttataat gctacctgtc cagtttgtgg tgggagtgct caacgcgaaa cggatactat
     5281 ggacaccttt gttgattcgt catggtattt tgctaagttt acttcaccgc attctacaga
     5341 aatatttgat tttgatgagg taaagtattg gacacctgtt gaccagtaca ttggcgggat
     5401 tgaacatgca tgtatgcatc tcctgtatgc acgattcttt accatggttt tgaatgattt
     5461 aggattatta cagtatc
//
LOCUS       DAOSOX010000157         5408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15242_length_5408_cov_11.969923, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000157 DAOSOX010000000
VERSION     DAOSOX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15242_length_5408_cov_11.969923"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2138)
                     /locus_tag="PLX22_09920"
     CDS             complement(45..2138)
                     /locus_tag="PLX22_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOT20254.1"
                     /translation="MKPAKIVKYSAIVLSIVLFVIAALMAAFVYLFPRELLKSIIVET
                     LQKSLNRPVSIGTIDYSLRGIQITDLYIYDTDNNKPFIAHAKDSAIRFQLLPLLQKQF
                     VINYIYLNNGAIHIIYYTENKIFTSNLAKLIHELLAKEESSIQTQIESIAIENTAIIL
                     ENPPEVLKPLEGRYSISTLCKLNESTIDLHNLSITMPQNRGKVTGDCAISKKGNFSIR
                     GDVVLHACDLLWVYGWSRHDLASILPYRIFSGTVSNLLITTHQVQGTATGSCKLASNA
                     MIYITNGFCNVDIDKKKVAIANVKGKVENSTFVLNQLIFNFDGDIQSIKVTDADIKLR
                     EIAPLLGNIDISSINADITGNASYTGKMIDADVTVSNVTIGKNGTILSINDKVTLIIK
                     NNQCKKDAIPAQVYSIPCVLSIATVDTSFKKFIINAEIQELNLNAPDITNIPGTGKSK
                     KEIAIPVELSGKITIHTLIKDKLAINNINCSYIMSRNIISISSFTAKIFGGDIAGRSI
                     ITLHPENPQINLVATFNTIRLQNISSYVEKYENRLFGNASGQLQVTIVPKEPLLDRII
                     GKIEFTIDNGKLANTGLQNGLSIWLADLKYKLSNLEFQKIYGNFHLNGPAITINSFIF
                     NAPAIRIKLNGKYHRHDESDIDIALEFSSQFIQDLPNPALLQLAKYKKGRWYIIPFKA
                     KGTDILNGKNIKQVQ"
     gene            complement(2116..3333)
                     /locus_tag="PLX22_09925"
     CDS             complement(2116..3333)
                     /locus_tag="PLX22_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT20255.1"
                     /translation="MVQEQFKVVNYLANSFIVVEGKRNADNFYIIRQGKVKIVKENPV
                     AQESNQLLGPGDFFGVIPCMSGHAHIEGAVAMTNVSLISVQRDQFGILIQKNPAVAMK
                     IIRFFSRKLREFDQAITRLTFANAVEEDPEHLFNIGQYYLKKKNLPHAAYALQRYIQH
                     CADGINRDKAITHLKSINAPFKVPENPQKNSLTRVYKDNQMIFCENEPGDELYIIQGG
                     KVKITKIVDEEVLLAVLKPGDIFGEMALLENRPRSASAITFGDTSLMAINRQNFETMV
                     QTQPQLATRLIQLLSERIWTAYRQLENLMIRDPLGRMYDTLLIQVEKQKIRITPKESY
                     TFDFGVKELLNMVGISHDKGDYLVVELLEDKNITLDEGKLICTNLEELEKTVNFYKKK
                     SALERKREASKNS"
     gene            complement(3564..3914)
                     /locus_tag="PLX22_09930"
     CDS             complement(3564..3914)
                     /locus_tag="PLX22_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20256.1"
                     /translation="MIEHVQSNSVKPVGGQFHKQKPSIITSNTDVLAPMKRFKKDLPI
                     PKMGATASSSMQKVLINQKEFYSHRGQVYEQLRNPRAAIASYQKATYYEPSDTNLSSH
                     IIKLKVKSLTNLKA"
     gene            complement(4095..>5408)
                     /locus_tag="PLX22_09935"
     CDS             complement(4095..>5408)
                     /locus_tag="PLX22_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20257.1"
                     /translation="ERILDAETNSKIDANINYLKKYRHKQEQQQRQRETVAKTTPNEI
                     KISLDSPIRLPEKIQIGFAPQDVDINNIIDEITKRILEKQRLQNTEDTAISNYSNEIK
                     QLSSHLEELKKEVASSLKELSSKEDALKTSEIQKLQNQLNDLTDRMFKLYEEKEKTRA
                     ELENLKTVHSSLLEGSKQPAVSTVKYEGKLPVELDPEPILKILEKLPKIAAPASKPID
                     KDRMKGDSAKDFLKKLYNDTVKQGETIQSSEIVEKTEKLKEDEEWELISDIIKDEKKS
                     QLDEISDEDIFKKILEEDKRKRADDIEIVGDYRKTDDVEYDIQDTETLHKLEEDRKFY
                     EKFLQHDRRKKKELPILRVTYDFKKLPDEFSLSREKNILEYAFYKYRPLLQKADELIQ
                     KRKVRDALNYYKVVMQQNIPPEFKSMIRKNIADLNEYLEKYLTSD"
BASE COUNT         1485 a          971 c          995 g         1957 t
ORIGIN      
        1 gggtggtttg atggctaaga ttaaagctga aattgcacaa tagattattg cacctgtttt
       61 atatttttcc cattcaggat atcggtgcct ttagctttaa atggtataat gtaccatctt
      121 ccttttttat atttagccaa ttgtaatagc gcgggattgg gaagatcctg tataaattgc
      181 gagctgaatt ccagtgcaat atcaatatca ctttcatcat ggcgatggta tttcccatta
      241 agctttatcc gtattgcagg tgcgttgaat ataaatgagt tgatggtaat agccgggcca
      301 ttaagatgga agttgccata tattttctga aattcaaggt tgctcagttt gtattttaaa
      361 tctgcaagcc atatgcttag tccattttgc agacctgtgt ttgctaattt tccattatca
      421 atggtaaatt caatctttcc aataattctg tccagcaacg gctcttttgg tacgatagtt
      481 acctgtaatt gtcctgatgc attcccaaac aggcgatttt catatttttc aacatatgaa
      541 ctgatatttt gcagccgtat agtattgaac gttgctacca gatttatctg aggattttca
      601 gggtgtagcg taatgatact tcttccagct atatcgccac caaagatttt tgcagtaaac
      661 gaagaaatgg aaataatatt tctggacatg atgtaactgc agtttatatt gttaatagca
      721 agcttgtcct ttattaatgt atgaatggta attttacccg agagttccac cggtatggca
      781 atttcttttt tagattttcc tgtgcccggt atatttgtta tatcaggagc attcagattt
      841 agttcctgga tctcagcatt aattataaat tttttaaatg aggtatccac cgtagctatg
      901 gaaagaacac agggtataga gtagacctga gcagggattg catctttttt gcactgatta
      961 ttttttataa ttaacgttac tttatcattt attgaaagta ttgtaccatt tttgcctatt
     1021 gttacatttg atactgttac atctgcatca atcatcttac cggtataact ggcattgcct
     1081 gtgatgtctg cgttgatgct gctaatatca atgttaccca ataatggagc tatctccctg
     1141 agtttaatat cagcatcggt aaccttgatg gattggatgt ctccatcaaa attaaaaatc
     1201 aattgattaa gaacaaatgt agaattttca accttccctt taacatttgc tattgcaact
     1261 tttttcttat cgatgtcaac attacaaaat ccattggtta tatagatcat tgcattgctg
     1321 gcaagcttac aacttccggt tgctgtgccc tgaacctggt gtgttgttat taatagattt
     1381 gaaacggtac cgctgaagat tctgtatgga aggatagaag ccaaatcatg tctgctccac
     1441 ccataaaccc agagtaaatc acaggcatgt aatacaacat ctccgcggat agaaaagttg
     1501 cccttcttag atatggcaca gtcgccggta acttttcctc tgttctgagg cattgtgatc
     1561 gatagattat gcaaatcgat agtgctttca tttaatttgc aaagcgtgct gatactatat
     1621 ctgccttcca ggggttttaa tacttctgga ggattttcaa gaataatggc tgtattttct
     1681 attgcaattg attctatctg tgtttgtatg cttgattctt ctttggcaag aagttcgtgt
     1741 atcagttttg ctaaatttga tgtaaaaatt ttattttctg tgtaatagat aatatgaatt
     1801 gctccattgt ttagataaat ataattgatt acaaactgtt tttgtaacag gggtagtaac
     1861 tgaaaacgga ttgccgaatc ctttgcatgg gcaataaatg gtttgttgtt gtcagtatca
     1921 taaatataaa gatcagtaat ctgtataccg cgcaggctat aatcaatggt accaatactg
     1981 acaggacgat ttaatgattt ttgcaatgtt tcaacaatga tggattttaa caattctctg
     2041 gggaaaagat atacaaatgc tgccatcaaa gcagcaataa caaacagtac tatggaaagt
     2101 actattgcac tgtatttaac tatttttgct ggcttcacgt tttcgttcta atgctgattt
     2161 tttcttgtaa aaatttacgg tcttttcaag ctcttccaga ttagtacata taagcttgcc
     2221 ttcatccaat gttatatttt tatcttccag aagttccacc accaggtagt cccccttgtc
     2281 atgggaaatc cctaccatat tgaggagttc tttaacgcca aaatcaaatg tatagctttc
     2341 ttttggtgta atacgtatct tttgtttttc aacctgaatc aataacgtgt cgtacatcct
     2401 tccaagagga tcgcgaatca taagattttc aagttgacgg tatgctgtcc atatacgctc
     2461 actcaacagt tgaatcagtc gggtggctaa ttgtggctgt gtttgaacca ttgtttcaaa
     2521 attttgcctg ttgattgcca taaggctggt atcgccaaat gttatagctg aagcacttcg
     2581 tggacggttt tcaagaagcg ccatctcacc aaaaatatcg cctggtttta atacagcaag
     2641 gagcacctct tcatccacaa tcttggttat ctttaccttc ccaccctgaa tgatatagag
     2701 ctcatcgccg ggttcatttt cgcaaaatat catctgattg tctttataca cacgtgtcag
     2761 actatttttc tgaggatttt caggcacctt gaatggagca ttgatggatt ttaaatgagt
     2821 tatagccttg tccctgttta tgccgtcagc acaatgttgt atataacgct gcagtgcata
     2881 tgctgcatga ggtaaatttt tcttttttag atagtattga ccaatgttga acagatgttc
     2941 aggatcttct tccactgcgt tagcaaaagt taaccgtgtt atagcctgat caaattcacg
     3001 cagtttacgc gagaagaatc ggataatctt catggcaacc gcaggatttt tctgtatgag
     3061 gatgccaaat tgatctcgct gtactgaaat cagtgataca ttggtcatgg caacagcgcc
     3121 ttcaatatgg gcatgaccgc tcatacaggg aataacacca aaaaaatcac caggtccaag
     3181 tagctgattt gattcctgag ctactggatt ttccttaaca attttaactt tcccctgccg
     3241 tattatataa aaattatcag cgttacgttt gccttcaaca acgataaaag aatttgcaag
     3301 gtagtttaca actttaaatt gttcctgcac catgtgttgc catcaatgtt tgatttgaag
     3361 tactgtaatc atagcatagt atactattgc taaaagtaaa caaaaaaatt attatcataa
     3421 atcccttatg gtaaaatcgg aattatggaa aataatatca agaattttta agggttaaca
     3481 atgaaaagaa tctgccatgt aattttttat acattatcca tacctatcat tgattgtagc
     3541 actcacttca accagtacaa aggttatgct ttaaggttgg tgaggctttt aacctttagc
     3601 tttatgatat gagaggatag gttggtgtca gatggttcat agtaggtagc tttctgatat
     3661 gatgcaatcg cagcacgcgg attgcgtagc tgttcgtata cctgaccacg gtgcgagtaa
     3721 aattccttct ggtttatgag aactttttgc atagaactgc tggcggttgc ccccattttt
     3781 gggatgggga gatccttctt aaaacgtttc ataggggcaa ggacatcagt attgcttgtt
     3841 ataatagaag gtttttgttt atgaaactgt cctcctacag gttttacact atttgattgc
     3901 acgtgttcta tcattgtact gcacctcact ataaagatac aaggaatata tgggtaaagt
     3961 caataaaaaa atgatacatt gtgattatga tttacaggca atgaaaccac atggtggttt
     4021 acacaaaaag tggtatcaat agtaacatca atagtgaaga caggcaatat gaagaataca
     4081 cattagccat atgcctaatc agatgtaaga tatttctcta aatattcatt aaggtcagca
     4141 atatttttac gtatcatgga tttaaattcg ggcggtatgt tctgttgcat cactaccttg
     4201 taataattca gggcatcacg tactttacgt ttttgtatca attcatcggc tttttgcagc
     4261 agcggtctat atttgtaaaa tgcatattcc agtatatttt tttctcgtga aaggctgaat
     4321 tcatcgggaa gttttttaaa atcataggtt acccgtaata ttggcaattc cttttttttg
     4381 cgtcggtcat gttgtaaaaa tttctcgtaa aatttacggt cttcttccag tttgtgtaat
     4441 gtttcagtat cctggatatc gtattcaaca tcatctgttt tacgataatc acctacaatt
     4501 tcaatgtcat cagcacgttt gcgtttgtct tcttcaagta tcttcttaaa tatatcttca
     4561 tcgcttatct catctaactg gctctttttt tcatctttta taatatcact tataagttcc
     4621 cattcttcat cctctttcaa cttttcagtt ttttcaacaa tctcggagga ttgtattgtt
     4681 tcaccctgtt ttactgtatc attatataat ttctttaaaa aatcttttgc gctatcacct
     4741 ttcattctgt ctttatcaat tggtttagag gcgggtgcgg caatttttgg tagcttttcc
     4801 aaaattttta agattggttc cggatcaagt tcaaccggca gcttcccttc atatttcacg
     4861 gtggaaacgg caggttgctt gcttccctct aaaaggctgg aatgcactgt ttttaaattt
     4921 tcaagttctg ctcgtgtttt ttccttttct tcgtataatt taaacatcct gtcggtgagg
     4981 tcattgagtt gattttgcaa cttttgtatt tccgaagttt ttaaagcatc ttctttgctt
     5041 gatagctcct tcaaggaact ggcaacctct tttttaagtt cttcaaggtg tgatgataat
     5101 tgtttgatct cattgctata gttgcttatt gctgtatcct cggtgttttg taaccgctgc
     5161 ttctcaagaa ttcgtttggt aatttcatca ataatgttat taatatcaac atcctgaggt
     5221 gcaaaaccaa tctgtatctt ttcaggtagt ctaatagggc tatctaatga aatttttatt
     5281 tcattaggcg tggtttttgc tactgtttca cgctgccgtt gctgttgctc ttgtttgtgt
     5341 cggtattttt tcaggtaatt gatgttggca tctatttttg agttggtttc tgcatcaagg
     5401 atgcgttc
//
LOCUS       DAOSOX010000158         5346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15461_length_5346_cov_6.051786, whole genome shotgun sequence.
ACCESSION   DAOSOX010000158 DAOSOX010000000
VERSION     DAOSOX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15461_length_5346_cov_6.051786"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..3534)
                     /locus_tag="PLX22_09940"
     CDS             complement(136..3534)
                     /locus_tag="PLX22_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOT20258.1"
                     /translation="MNRLTSDEATELYDEIRSKCLFESNRDTLYVKTLMLRPVLEKMF
                     YYLTRDINKIYLYNNSERMEYIFNHDNVDADVLKNARILRKFLNRFHHFNETNEKSNN
                     SGNDCLTNENYWLCIKTLCNLVHYFSKVEIPPDLKELYKDVQNLKWNMKPEEPKEKID
                     YIRLLINTKDAHHNVLRCSDIHGDEKLLYLKNEKESRYFNVLNIAWQCSTIDCYNIEK
                     LADKPWYTDNQNSIIILEPDYLVDATVIVNCFENNKPNPMFYLVEKFTNDNDVKESMI
                     LGSIVNTLLDEIIVNKAQGFNKCCIKAMADYCLDIFCLFKDDEEYNNTLIKRLHDTAK
                     EHYNNILQYFKDYEKDSIYHIEPYFISPQYGLQGRLDLLVQRNSNKNYNDIFELKSGS
                     VPDIDAWHEHRVQVYCYHLLVESCYHNRAGNNYVFYSRLQQPARDIQINKALVNDLMM
                     VRNHIVSYEYQLATGNFDIVAFANKFKKYFASHQDTIKKFRKTAYENFISTITKLQPL
                     EIAYITNFIRFIATEHLIAKTGDGNEHGFSSLWRSTQKEKADNFLLLTDLEYDSDSSS
                     IKNNVVVFKRGTLFTHTTVFREGDICIVYPDIPANDDHFAYVKHFYYRATILELNNEY
                     VKVRLRNNIKETRELKTFKWCIEKDLIENNYTKLYKSLFEFMESNIKDILLGLKEPEF
                     HNHDNTILHTGKDEDINEIISLALNASDYFCIQGPPGTGKTRVVLKEIVNNLYASTDE
                     NIMLLACTNRAVDEICLALQKSSLPYIRLGKSSDDSIESLDTLKNRINNEKDLKNVYD
                     RIINQRVFVSTVSTAQQNRIFNLKKFTTVIVDEASQLLEPDIVGLLTKFKRFILIGDE
                     KQLPPVVTQDENLTKVNNPHLEKICLYDLSMSLFERLILNAKKNKWHKAYRTLKNQKR
                     MHNDIAEFINRHFYDSALATVKDDSAPYTVTFNKNSHNNYEKVLANNRMVFIESKKEP
                     GSKKENTCEAAIVVDLIKLIINTYNSKFKSDTVGVITPFRAQINCIKKKLLDSGVDDA
                     YLSNIMIDTVERFQGSERDIIIVSLAVNSSNYLESITSFNFNKRVDRKLNVTLTRGKE
                     YVIMIGCSEILQQHEHYRLLIEHIKNKGGYANYENLCEPLLFHR"
     gene            3887..4390
                     /locus_tag="PLX22_09945"
     CDS             3887..4390
                     /locus_tag="PLX22_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20259.1"
                     /translation="MSKKKNNKQLNTDDNNFWLQYAQQLENSKPMERKWITHETHDYV
                     IDDGEDKNADINMTILYTKEQATPKSKRLLFLVYKFIGFLKSLKNSIIKKKHFFTCKY
                     AKSILTPANITIMFSKKIVLLESESGEKIWIIINPANEDILVTIKFKFYETQMWLRKN
                     TIMGWTE"
     gene            complement(4694..5203)
                     /locus_tag="PLX22_09950"
     CDS             complement(4694..5203)
                     /locus_tag="PLX22_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20260.1"
                     /translation="MQNAETFFGTVRNIGYKSKQKGIPIYTEMTGCELLLGKEYTATG
                     YITIDSNQLKLFLQKNNFHNSEQVLQMFSAPYILGSLKDFYKEQLNNKKHPVSQDIVK
                     CIQKYVDANYVPIRMGRFSQVESKTFKITRTWDKGQNRSMNIEGGASRNYIKDTIGAG
                     WGALALKKA"
BASE COUNT         1761 a          913 c          739 g         1933 t
ORIGIN      
        1 tttatatagt agcattgtca aggataaaat gcaagcaaag gaaaataagg aatgggaacg
       61 gtaagataag caggcattgc gctatggcgt tgttataatt taaaaaacac tgcaaccgtg
      121 aaaataccca atctgtcacc ggtggaataa caatggttcg caaagatttt cataattggc
      181 ataaccgcct ttattcttta tatgctcaat gagcaatcta taatgttcat gctgctgcaa
      241 aatttcggaa caaccaatca ttattacata ctccttccca cgggttaatg ttacatttaa
      301 ctttctatcg acacgtttat taaaattaaa tgatgttatt gattccagat aattactgga
      361 attaaccgcc agtgaaacaa tgataatatc ccgttcactt ccctgaaatc gttcaaccgt
      421 gtcaatcatt atattgctta aatatgcatc atccactccg gaatccaata attttttctt
      481 gatacaattg atttgagccc taaatggcgt gatgaccccc actgtatcac tcttaaactt
      541 actattatag gtattgataa tgagcttgat taaatcaacc actatagccg cttcacaagt
      601 attttctttt tttgaacccg gttccttttt tgattctata aataccattc tattgtttgc
      661 aagtaccttt tcatagttgt tatgcgaatt tttattaaaa gtaactgtat acggtgcgct
      721 atcatctttt actgtagcta atgcgctgtc ataaaagtgc ctgtttataa attctgcaat
      781 atcattatgc attcgttttt gattttttag cgttctatat gctttatgcc atttattttt
      841 tttagcattt aagatgagcc gttcaaatag cgacattgat aaatcatata aacaaatttt
      901 ttctaaatgg gggttgttta cctttgttaa attttcatcc tgagttacaa caggtggcaa
      961 ttgcttttca tcaccaatca atataaatcg tttaaacttt gttaacaaac caacaatatc
     1021 gggttccagt agttgcgaag cctcatccac tattacagta gtaaattttt taagattaaa
     1081 tatcctgttt tgttgtgctg tactcactgt gctgacaaaa accctctggt ttatgattcg
     1141 gtcatacaca ttttttaaat ctttttcatt gtttattcta ttttttaatg tatccagcga
     1201 ctcaattgaa tcatcactac ttttacccaa acgaatatag ggcaaactgg atttttgcaa
     1261 ggctaaacaa atttcatcaa cagctctatt ggtacatgcc agcaacatta tattttcatc
     1321 agtagatgca tacagattgt taactatttc tttgagcaca actcgtgttt ttccggtacc
     1381 gggagggccc tgtatgcaaa aataatcgct ggcattcaat gctaatgata ttatttcatt
     1441 gatatcctcg tctttaccag tgtgtagtat agtattgtca tgattatgaa attccggctc
     1501 ttttaaaccc agtaatatat cctttatatt gctttccata aattcaaaaa gagatttata
     1561 taacttggta tagttgtttt caattaaatc cttttcaata caccatttaa aagttttcaa
     1621 ttcccgtgtt tcttttatat tatttcttaa acgaaccttc acatattcat tattcagttc
     1681 aagtattgta gctctataat aaaaatgttt tacatacgca aaatgatcat catttgcagg
     1741 gatatccgga tatactatgc atatatcacc ttctctaaaa accgttgtgt gcgtaaataa
     1801 agttccccgt ttaaatacca caacattgtt tttaatgctg gagctatcac tatcatattc
     1861 taaatcggtt aacagtaaaa aattatctgc tttttccttc tgtgttgaac gccataatga
     1921 tgaaaaacca tgttcatttc catcccctgt ttttgcaata agatgctcgg tagcaataaa
     1981 gcgaataaag ttagtaatat atgcaatttc aagtggttga agctttgtaa ttgtagaaat
     2041 aaaattttcg tatgcagttt ttctaaattt tttaattgta tcctgatgcg atgcaaaata
     2101 ttttttaaat ttatttgcaa atgcaactat atcaaagtta ccggttgcta attggtactc
     2161 atagcttaca atatgattcc taaccatcat taaatcatta acaagcgctt tgtttatttg
     2221 tatatcgcgt gctggttgct gcaacctgct atagaaaaca taattattac cagcacggtt
     2281 atggtaacat gattcaacca gaagatgata gcaataaacc tgtactctat gctcatgcca
     2341 tgcatcaatg tcaggtacac tacccgattt taattcaaaa atatcattat aatttttgtt
     2401 gctgttacgc tgaactaata gatccaatct accctgtaaa ccgtactgtg gagatataaa
     2461 atacggttct atatgatata tactatcttt ttcataatct ttaaagtatt gcaaaatatt
     2521 gttatagtgt tcttttgccg tatcgtgtaa tctttttatc aaagtattat tatattcctc
     2581 gtcatcctta aataagcaaa aaatatccag acaataatca gccattgctt taatacaaca
     2641 tttgttaaat ccttgtgctt tattaactat tatttcatct aacagagtgt ttacaatact
     2701 accaagaatc atgctttctt ttacatcatt atcattagta aatttttcaa ctaaataaaa
     2761 catgggattg ggtttattgt tttcaaaaca attaactatc actgtggcat caacaaggta
     2821 gtcaggctct aaaataatta tagagttttg gttgtcagta taccatggtt tatcagctaa
     2881 tttttcaatg ttatagcaat ctatggtgga acattgccat gctatattca atacattaaa
     2941 atagcggctt tctttttcat ttttcaaata aagaagcttc tcgtcaccat gtatatcaga
     3001 acaacgcaat acattatgat gggcatcttt tgtattaatc aataaacgta tataatcaat
     3061 tttttccttt ggctcttctg gcttcatatt ccattttaaa ttttgaacat ctttatacaa
     3121 ttcttttaaa tcaggtggta tttcaacctt tgaaaaataa tgaactaaat tacaaagtgt
     3181 ttttatacac aaccaataat tttcgttagt caaacaatca ttaccactgt tgttactttt
     3241 ttcattagtt tcattaaaat gatgaaatct atttaaaaat ttccgtagta ttctggcatt
     3301 ttttaataca tctgcatcta cattatcatg gttgaaaata tattccattc tttcagaatt
     3361 gttatataaa taaattttat tgatatctct ggttaaataa taaaacatct tttccagaac
     3421 aggtcgtagc attaatgttt tgacatacag tgtgtctctg tttgattcaa ataagcattt
     3481 gctgcgtatc tcatcataca attcagtggc ttcatcactt gttaacctgt tcatataatc
     3541 acacctcatg ttacacaatc attgactatt ataaggtttt taacccatag cagtatagta
     3601 tcgccatggt tttgcctttt ttaccacatg ctattgcaca aaacagtatg gtatggtaag
     3661 tttatataaa ttacaagtaa aaaagttacc aatttttaac ctatattttt tactattctg
     3721 tgtctaatgt agtaaatgga catctgcatg gcatgcaata aaccatgcag atgtcccact
     3781 gctgtcattc taagtaaagt ttaagaatcc cgttgagggt aaaaattctt tatgacttca
     3841 tccagtagca tgacattggt acaaaatttt atatcggagg gatattatga gcaaaaagaa
     3901 aaacaataaa caattaaaca ctgatgataa taacttctgg ttacaatatg cacagcaatt
     3961 agaaaacagt aaacccatgg aaagaaaatg gataacccat gaaacacatg actacgtaat
     4021 tgatgatgga gaggacaaaa atgcagatat aaacatgacc atattgtata ccaaagagca
     4081 ggctactcca aaaagtaaaa ggctactgtt tttagtttat aagtttattg gttttttaaa
     4141 atcgttgaaa aactccataa taaaaaagaa acatttcttt acatgcaaat atgcaaaaag
     4201 tatcttaaca cctgccaata taacaattat gtttagtaaa aagattgttc tcctggaaag
     4261 tgaatccggg gaaaaaatat ggattattat aaatcctgct aatgaagata ttctggttac
     4321 gataaaattt aaattttatg aaacccaaat gtggttacga aaaaatacca ttatgggctg
     4381 gacagaataa ggttataata gtttaccatg aaaccatacc catagctaat ggagtaccat
     4441 aatcatagta ttgataccta ttgtgagtga ttgtagtgat aacaaaatac taaattcttt
     4501 ttcaatataa aatttttctt gattttttga ttcatacatt ataaattgca cgctaattat
     4561 aagttaaaaa ttctataaat caccaatcaa ataatgaaaa gtttgccatg gaatttatta
     4621 aaaatataga atttctagtc caccccgaat tagcaaaaaa aatggagaat gacaaaaagt
     4681 attgtacagt tcactatgct ttttttaaag ccagcgcccc ccagcccgca ccaattgtgt
     4741 ctttgatata atttctgctt gcaccacctt caatattcat acttcgattt tgtcctttat
     4801 cccatgtacg agttatttta aatgtctttg attcaacctg cgaaaatctt cccatgcgta
     4861 tagggacata attagcgtcg acatattttt gtatacattt tacaatatcc tggcttacag
     4921 gatgtttttt gttattcaat tgttctttat aaaaatcttt taatgatcca agtatataag
     4981 gtgctgaaaa catttgcaat acttgttcac tattatgaaa attatttttt tgtaaaaata
     5041 gcttcaattg attactatca atagttatat aaccggtagc agtatattcc ttacctaata
     5101 gtaattcaca acctgtcatc tcagtgtaaa taggaatacc tttctgcttt gatttatagc
     5161 caatattcct tactgtgcca aaaaatgttt cagcattttg caaagtaaaa tctgatactt
     5221 ttaaaaatct aaacggatct aaaaattttg tcccattgtt tgttgaaatt gaataacacc
     5281 tccatttcta ctatgcaatc ttattatttt cttcaatcac ttttttccca aacattgcaa
     5341 tattta
//
LOCUS       DAOSOX010000159         5319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15569_length_5319_cov_7.178571, whole genome shotgun sequence.
ACCESSION   DAOSOX010000159 DAOSOX010000000
VERSION     DAOSOX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_5319_cov_7.178571"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1162)
                     /locus_tag="PLX22_09955"
                     /pseudo
     CDS             complement(1..1162)
                     /locus_tag="PLX22_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            complement(1246..3141)
                     /gene="gyrB"
                     /locus_tag="PLX22_09960"
     CDS             complement(1246..3141)
                     /gene="gyrB"
                     /locus_tag="PLX22_09960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT20261.1"
                     /translation="MTSYRADKIQVLEGLEAVRKRPAMYIGSTDINGLHHLVYEVVDN
                     SIDEALAGFCDAISITINNDNSVEVIDNGRGIPVDIHPVYKTSALEIVLTKLHSGGKF
                     DNQSYKVSGGLHGVGISVVNALSEYMEVEVYKEGRKYFQSYKRGVPTDKVKDLGETKK
                     TGTRVLFRPDKQIFEETEFHFETLAKRMRELAFLNSGIKITLKDSRGKGREHLFYYKG
                     GIVSFVQSLTENKTSITKKVIYLHDTRNNVDVEIALDYIDTYTETVYCYANNIHTKEG
                     GTHLIGFKSALTRVLNEFLRREGYDKKIPQLSGDDVREGLVAIISVKIPNPQFEGQTK
                     MKLGNSEVKGIVESITNEHLTQYFEENPQAVRKILEKCIDTARSRIAAKKARDLTRRK
                     NALENDTLPGKLSDCSERDPSLCELYLVEGDSAGGSAKQGRDRRFQAILPLKGKILNV
                     EKSRLDKILSNEEIKTIITALGAGIGESEFDIAKCRYHKIIIMTDADVDGSHIRTLLL
                     TFFFRYMPEIIANGYLYIAQPPLYNIKAGKEQYYAYSDEERDSLVKKLDKSKVAIQRY
                     KGLGEMNPEQLWETTMDPEKRTLLQVTLEDEVEADEVFSVLMGDAVEPRRKFIEENAH
                     MVENLDL"
     gene            complement(3138..3377)
                     /locus_tag="PLX22_09965"
     CDS             complement(3138..3377)
                     /locus_tag="PLX22_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20262.1"
                     /translation="MFVHIGDRKAVSDTKLVAILNCDTVAKSPGMNAILLQNIKGDDK
                     TMAVCIDSIVTTRVSSYTVIKRYGQINDTVWSKKL"
     gene            complement(3358..3720)
                     /locus_tag="PLX22_09970"
     CDS             complement(3358..3720)
                     /locus_tag="PLX22_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOT20263.1"
                     /translation="MKFRYKEKRYHRTVSYNDIVNDVISEIGLSDDLIMEQIRSLWPN
                     VVDDIIATHSYPYKIVNKVLYVMVDHPIYANELSLSQAVILKKCSEFVNFLSVNAVKC
                     TIGREKYSKKRRNVCPHR"
     gene            complement(3717..4817)
                     /gene="recF"
                     /locus_tag="PLX22_09975"
     CDS             complement(3717..4817)
                     /gene="recF"
                     /locus_tag="PLX22_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOT20264.1"
                     /translation="MYISRLLLKSFRNYENETIFFSPGINIITGKNGSGKTNILEAVY
                     ILSYVKSFKNCLDSDIVTWGNHEYFICVDVKNSHNLTIEAGVGIEDHHSIKKVKIDSK
                     EIKKLTEFYGIIKLVAFFPDDISIIYGSPDTIRKYYDRFFSKIDREYLELLSNFKQIL
                     KNRNKLLKVLEFKRDIIKQLDIWDTMYSDCVYKISKKRVQYINIFNQHFNKVYKEISG
                     FDDNLEIIYTTQCDNNSLQSIQQMLLKKRNIDIQWGTTTIGPHRDSYCVMGMNKKFFK
                     SYASTGQRRLASIAMKMAEVNIVDAMSGQVSIVMLDDVLSELDEERRINVIHKLTINN
                     HQIIITCANMSDIQFINNYKLFNVINNRVVEQ"
     gene            complement(4824..>5319)
                     /locus_tag="PLX22_09980"
     CDS             complement(4824..>5319)
                     /locus_tag="PLX22_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20265.1"
                     /translation="ILNEHDTCLIEFDESRCHIKSGRTEIVSRLIDGQFPDYKQVIPK
                     DYLLKSVVEVKRLKEALNRIKIFTNEPSWRILLKFSSNVLVIEASYPDQGEGREEVPV
                     ESDANEPVTVGINVQFLLDLLKEIDSYSVFIGVTGVMSPLVLHPEDNKEFLSVIMPIQ
                     IRSM"
BASE COUNT         1584 a         1109 c          752 g         1874 t
ORIGIN      
        1 tcgcaatcac ttcatcaata ttgtctaaag ctatttttaa accttctaat atatgagccc
       61 gctcctttgc ctttgccaga tcatactgag tacggcgtgt tacaacaatc ttgcgatgat
      121 tgatataatg gaatattaat ccttttaaat ccagcagttt tggttgatta ttaacaagag
      181 ccagtaggtt aatcccaaat gacgtctgta attgtgtatg tttatagagc tgattgatga
      241 taatgtttgt tactgcatcc tttcgacatc ctataacgat gcgtaatcct ttacgatccg
      301 actcatcccg cagttcattg ataccttcaa taatcttatt gtttaccagt tcagctattt
      361 tcgcaataag tgctgcttta ttcacctgat agggtaattc tgtaattaca atcagcgatt
      421 tatttttatg ttcttcaata tgcagtttgg atctaacgac tatacttccc ttccctttag
      481 catatgcctt caacaattct tctgaagcaa caatcgtagc tccagtaggg aaatcaggtc
      541 ccttaacaaa tttcatcagt tgcttaaccg ttgcatcagg cgtatcaatc atatagacaa
      601 tcgcatcaat tatttcgcct aaattgtgcg gagggatatt ggttgccata ccaacggcaa
      661 tacctgaaga accattaacc agtaaattgg gaaaagccga agggagtaca acaggctctt
      721 ttcgggtttc atcaaaattt gatacaaaat ctacagtatc tttatcaata tctttcagca
      781 attcctgtgc aattggtgaa agacgagctt ctgtataacg atatgcggct ggaggatctc
      841 catctacaga accaaaatta ccctgaccat caatgagtgg taatctcatc gaaaaatcct
      901 gtgccattct aacaagcgta tcatacactg cggcatcacc atggggatga taattaccaa
      961 taacttcccc tactatcttt gctgatttta catatggtct gtcactgcgc catgcacgct
     1021 cattcattgc atgtaagata cgccggtgta caggtttcag gccatcacga acatccggca
     1081 gtgctcgtcc aacaatgacg ctcatggcat aatcaagata tgagcgtttc attgattctt
     1141 caatctctat atttaccact tttttgtctt tttttacatc ttccttcatg ttaactattt
     1201 acctattatt tgaaaagatt tctgcataat gctcgatagt tatggttata aatctaaatt
     1261 ctctaccatg tgtgcatttt cttctatgaa ttttcgtctt ggttcaactg catcacccat
     1321 taaaacagaa aaaacctcat ctgcttctac ctcatcctcc agagtaacct gcagcaatgt
     1381 ccttttctca gggtccatgg ttgtttccca taattgttcg ggattcattt cacctagccc
     1441 tttgtatcgt tgtattgcaa cttttgattt atctaatttt tttactaagg aatctctttc
     1501 ttcatcactg taagcatagt attgttcttt gccagcctta atattataca atggaggctg
     1561 agcaatatat aagtatccat tagcaataat ctcaggcata tatctaaaaa agaaagtgag
     1621 caataacgta cgtatatgtg atccatcaac atcagcatct gtcatgataa taatcttatg
     1681 ataccgacat tttgcaatat caaattcact ttcaccaata cccgcaccta aggcagtgat
     1741 aatagtcttt atttcttcat ttgaaagaat tttatctaac cgcgatttct caacgttcag
     1801 aatctttccc tttaagggca aaatagcctg gaatcttcta tctctcccct gtttagctga
     1861 accacctgca gaatcaccct caaccagata aagctcacac aatgacggat cacgttcact
     1921 gcaatccgaa agcttgcccg ggagagtgtc attttctaaa gcattttttc ttcttgtaag
     1981 atctcttgct tttttggcag caattcggga gcgtgctgta tcaatacatt tctcaagaat
     2041 tttacgtact gcctgtgggt tctcttcaaa atattgtgta agatgctcat tggtaattga
     2101 ttcaactatg ccttttacct cactattacc cagcttcatc ttggtctgcc cttcaaactg
     2161 cggattagga atcttaacgc tgataattgc caccagaccc tcacgaacat catctcctga
     2221 aagctgggga atctttttgt catatccttc acgccttaaa aattcgttta atacacgtgt
     2281 taatgctgat ttaaagccta taagatgggt cccaccttct tttgtgtgga tgttatttgc
     2341 atagcaatac accgtttctg tataagtatc tatataatca agggctatct ctacatctac
     2401 attattcctg gtatcatgta aatatatgac ttttttggtt attgatgttt tattttccgt
     2461 taaactctga acaaacgaaa caataccacc tttataataa aagagatgtt ccctgccttt
     2521 accccgtgaa tcctttaacg ttattttaat gccactattc aggaaagcca gttctctcat
     2581 ccttttggca agtgtttcaa agtggaattc tgtctcttca aaaatctgtt tatcaggcct
     2641 gaaaagtact cttgtacctg ttttttttgt ttcccctaaa tctttaacct tatcagtagg
     2701 aactccccgt ttatagctct gaaaatattt tctgccttct ttatatactt ctacttccat
     2761 atattctgag agtgcattaa caaccgatat accaacacca tgtagacctc ctgatacctt
     2821 atacgactga ttatcaaatt taccaccgga atgtaatttt gtaagaacta tctccaatgc
     2881 agacgtttta taaacaggat gtatatccac cggtatacca cgcccattat ctataacctc
     2941 gacagaatta tcattgttta ttgttatact gatagcatca caaaaaccag ctaaagcctc
     3001 atctatagaa ttatcaacaa cctcgtaaac aagatggtga agcccattaa tatcagtgga
     3061 accaatatac attgcagggc gtttacgtac tgcttcaagt ccctctagaa cttgaatttt
     3121 atctgctcta taacttgtca taatttttta ctccatactg tatcgtttat ctgtccatac
     3181 cgtttaatca cagtatatga actgaccctt gttgttacta tgctatcaat gcacacagcc
     3241 attgttttgt catcaccctt aatattttgc agcaggatag cattcatacc gggtgactta
     3301 gccacagtat cacaatttaa tattgcaacc agctttgtat cagatacggc ttttctgtca
     3361 cctatgtgga caaacatttc ttctcttctt gctatatttt tcccttccaa tggtacattt
     3421 taccgcattc actgacaaaa agtttacaaa ttcagaacat ttttttagaa ttactgcttg
     3481 tgataaactc aattcatttg catagatggg atgatcaacc ataacatata ataccttatt
     3541 tactatttta taaggatagc tatgtgttgc aataatatca tctactacat tgggccataa
     3601 agaacgtatt tgttccatta tcagatcatc cgacaaacca atctcagaaa taacatcatt
     3661 cacaatatca ttatatgaaa cagttctatg gtatcgtttt tctttataac gaaatttcat
     3721 tgttcaacta ccctgttatt tataacatta aaaagcttgt agttattgat aaattgaata
     3781 tcgctcatat ttgcgcaggt tataataatc tggtgattat tgatagtaag cttgtgtatt
     3841 acatttattc gtctttcttc gtccaattct gataatacat catcaagcat gacaatagat
     3901 acctgaccac tcatagcatc aacaatgtta acctccgcca tcttcatagc tattgaagct
     3961 aatcttctct gccccgttga tgcatatgat ttaaaaaatt ttttattcat ccccattaca
     4021 caataactat ctctatgagg tcctattgtt gtagtccccc attgaatatc aatatttctt
     4081 ttttttaaca acatttgctg tatcgattgt aaagagttat tatcacattg agtggtatat
     4141 ataatctcaa gattatcatc aaatcctgaa atctctttat acaccttgtt aaaatgctga
     4201 ttgaatatat taatatactg tactcttttt ttagatatct tataaacaca atcagaatac
     4261 attgtgtccc agatgtcaag ctgtttaatt atgtctcttt taaattcaag aacttttaat
     4321 aatttattgc ggttttttaa tatttgtttg aagttactta aaagctcaag atactccctg
     4381 tcaatttttg aaaaaaaacg atcataatat tttcttattg tatcaggtga tccataaatt
     4441 atggatatat catccgggaa aaaagccact aacttaatta taccataaaa ttctgttaat
     4501 ttctttattt ctttgctatc tattttaact tttttaatac tatgatgatc ttcaattcct
     4561 acacctgctt caattgtcaa attatgtgag tttttcacat caacacagat aaagtattca
     4621 tggttacccc atgtaacaat atcactatct aaacaatttt taaacgattt gacgtacgac
     4681 agtatataaa cagcttctaa tatatttgtt ttacccgaac catttttccc ggtgataata
     4741 tttattcccg gtgaaaaaaa aattgtctca ttttcataat ttcgaaaaga ttttaataac
     4801 aatctggata tatacattac ctgttacatt gaacgtatct gaatgggcat tatcactgaa
     4861 aggaactctt tattgtcttc aggatgtaat accaatggac tcataactcc tgttacccct
     4921 ataaaaactg aatacgaatc tatctctttt aacaaatcaa gtaagaattg aacattaata
     4981 ccaaccgtta caggctcgtt agcatccgac tcaacaggca cttcctccct accctcaccc
     5041 tgatcaggat agcttgcttc tattacaaga acatttgatg aaaattttag aagaattctc
     5101 catgatggtt catttgtgaa tatttttatt ctgttgagtg cctcttttaa ccttttaacc
     5161 tcaactactg atttcaacaa ataatctttt ggtataacct gtttataatc aggaaattgc
     5221 ccatcaatga gccgtgaaac aatttctgtt ctacctgact ttatatggca tctactctca
     5281 tcaaattcaa taagacatgt atcatgttca ttcaagata
//
LOCUS       DAOSOX010000160         5278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15735_length_5278_cov_9.926479, whole genome shotgun sequence.
ACCESSION   DAOSOX010000160 DAOSOX010000000
VERSION     DAOSOX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_5278_cov_9.926479"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLX22_09985"
     CDS             <1..478
                     /locus_tag="PLX22_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20266.1"
                     /translation="PSKSHRRTQRKNIQTTVKVSELSLTRRIYDIYGDHSLSRFGQPP
                     SRQFLISNVLDICTNTYQIEYYIHSTLFAVDYIDETNKALNTIYCIFHSDFAHLSPGI
                     FSILYAIQLAQDKNLPYLYLGYYVPGCTVMEYKISFHPYQWYSWQKKQWLDEPVQL"
     gene            complement(480..821)
                     /locus_tag="PLX22_09990"
     CDS             complement(480..821)
                     /locus_tag="PLX22_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20267.1"
                     /translation="MNNYNLLFNEAIQQRLHSSQWDKAMALQVMAKVKKRNRLYTAFA
                     SAAAMILFAAAVTLWQYNSDDSTNNAMYSFVHAQVNGVYTTVFPEANNADMLYGDDRV
                     GDIIETSLFER"
     gene            complement(818..1309)
                     /locus_tag="PLX22_09995"
     CDS             complement(818..1309)
                     /locus_tag="PLX22_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT20268.1"
                     /translation="MDASEFTAIVHETKDGVLRAVQKHLAAQFYEAIDDVVQETYLRA
                     YKSLAAGKFEGRSTLDSWLYRIAANESKRMNGKLLREQKKLQKLSRATTAYSPAIDCE
                     ELSLMPVIEMLPDKYKDVVKLKAQGNNEWEIATKLNIPKGTVKSRLSRGKDLLLRLLQ
                     EEL"
     gene            complement(1328..1843)
                     /locus_tag="PLX22_10000"
     CDS             complement(1328..1843)
                     /locus_tag="PLX22_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOT20269.1"
                     /translation="MKRISLAFVVIMLVTSVAFGGEPKGRGHDCPDMQMKKGPIFGDV
                     ERLKQELGLSDAQVDKIIAINKQYGMKMLDIREKLAPNVIQLKKLLLQDDVDINAVRA
                     KLKEIADLRVELHLLRIQHVIDIEKQLNKEQLNKLRNYKRAEMKKMKKMHPGGDCGPM
                     GPEGPDDGPMM"
     gene            2068..2913
                     /locus_tag="PLX22_10005"
     CDS             2068..2913
                     /locus_tag="PLX22_10005"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT20270.1"
                     /translation="MVNLSQPVLKTISSRYSCRTYTNEELKDEDYDYLQQCIAAISTL
                     SGTTLRITLIHNNRVKLPQKIGTYGFIKNPQGFIAATTTDTKEGLVDFGYALEQMVLA
                     ATERSIGTCWLGGTFTRGPFNQIMGLLPHEIVPAVISLGYPADKKRMFERSVRYIAGS
                     DTRKKADELFFEHTWNNPYKGSEPFITLLELVRLAPSASNKQPWRILFNNNAFHFYLE
                     RTPGYYDRNRALLGFHDLQMIDMGIALCHFHYGTIDYKIDGRFAVTHHPAIDKREYII
                     SYVLG"
     gene            3096..4676
                     /locus_tag="PLX22_10010"
     CDS             3096..4676
                     /locus_tag="PLX22_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT20271.1"
                     /translation="MDTQYTSRQILEIKSSGTYIILFILIALIAHVFQFIAKVDLFEI
                     LKLSFASIICLLIAYSIYKRKKTLKRTEILEWLLGFITLTIPLAAKFAYATTYDWTFA
                     LESYNSSILLIIFMVLLQLYFNKRLVAFYSSYAIVTWVAFLLLAIAHGADIHWDATID
                     GKPIHGFIIFREIFMIVITGIFGILAYKNIQIVEQYDDRSTRQLSIIHGQSEKQKQLN
                     EIIKEKVSDLLQQVQRQDELTIMFNDKMQNQASTFEEISATLEELLGSAESIAHSSEE
                     QVGGNTMMETIVSEFKIIKNETRQNLDKTIHEVDNVVAQTGSANERIRDVESTINEIR
                     NQSQKIAETIGVIVDIADRINLLSLNASIEAARAGDYGRGFAVVADEIGKLAYQTSES
                     IKDIESVLMQSTKTTEKGVEVIRTTAAMLKELIAKMAESSSKIKILQDSTTVEEKYIK
                     NIIAQMEKNVELAKNIGTGTEEQKAAIESSARAIEHMNEVVSQMVDEIQKLAMTSRSI
                     LDNATALLEQSKGDSSSE"
     gene            4713..>5278
                     /locus_tag="PLX22_10015"
     CDS             4713..>5278
                     /locus_tag="PLX22_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOT20272.1"
                     /translation="MEQKYYQYILFCDFDGTITAEETFVGMCQKFAPDTFHEIGKRMW
                     ERQLTIREGVRAVIESLPSSLYPAMLAYIDAIDMRPGFPEFLCYCNNHSIPVVIISGG
                     LYDCVVRKLGDLINKVEAIYAAKVDASGDYLKLYSDYEYEGELVAKAVIMDNYRAQCR
                     IAVGDGPTDIEMAKHADIVFARSMLKQF"
BASE COUNT         1635 a         1071 c          993 g         1579 t
ORIGIN      
        1 accatcaaaa agccatcgca ggacacagcg aaaaaatata caaacaaccg taaaagtgag
       61 tgaactttcc ttaaccagaa ggatctacga tatttatggg gatcattcat tatcgcgttt
      121 tggccagccg ccatctcgac aatttttaat cagcaatgtc ctggatatat gtaccaacac
      181 ctatcaaatt gaatactaca tacattccac cctctttgcc gttgattaca ttgatgaaac
      241 taacaaagcg ctcaatacaa tctattgtat ttttcattct gactttgcac acttgagccc
      301 cggtatcttc agcatcctgt atgcaataca actggctcaa gataaaaatt taccttatct
      361 ttatttagga tattatgttc ccggctgtac ggttatggaa tacaaaatct catttcaccc
      421 gtatcagtgg tatagctggc aaaagaaaca gtggcttgat gaacctgtgc aactataaac
      481 taccgctcaa ataacgatgt ttcaataata tcacccactc tatcatcacc atacagcatg
      541 tcagcgttgt ttgcttcagg aaaaacagtc gtatacaccc cgttaacctg agcatgtaca
      601 aagctgtaca tagcattatt ggtgctatca tcagaattgt actgccacag ggtaacagca
      661 gccgcaaata gtatcatagc cgctgccgag gcaaaagcag tatataatct atttcttttt
      721 ttaacctttg ccattacctg caaagccata gctttatccc actgtgaact gtgcagccgc
      781 tgttgaattg cttcattaaa aagtagatta taattgttca tagttcctcc tgcaacaagc
      841 gtaataataa atccttccct cgtgacagtc gggatttaac agtgccttta ggaatattga
      901 gctttgttgc aatctcccat tcattgttac cctgtgcctt cagttttact acatctttat
      961 acttatcagg aagcatctca atgactggca taagcgatag ttcctcgcaa tctattgcag
     1021 gtgaatatgc ggttgttgcc cttgataatt tctgtagctt tttctgctcc cgtaataatt
     1081 taccattcat gcgttttgac tcattggcag caatcctgta tagccatgaa tcaagcgttg
     1141 accttccttc aaacttaccc gctgcaaggc ttttatatgc ccgtagatat gtttcctgca
     1201 caacatcatc aattgcctca taaaactgcg ctgcgaggtg cttttgcact gctcgcagca
     1261 caccatcctt tgtttcatgt actattgccg taaattcact tgcatccatc aggataagcc
     1321 tttgccccta catcattggg ccatcatcag gaccctcagg acccattggt ccacaatcac
     1381 cgccggggtg catctttttc atctttttca tctctgctct tttatagttc cttagtttgt
     1441 taagttgttc cttattaagc tgcttttcaa tatcaatgac atgctgaatt cgtaaaagat
     1501 gtagctctac ccgcaaatct gctatctctt ttagtttagc acgcactgca ttaatgtcaa
     1561 cgtcatcctg aagcaaaagc ttttttagct gaataacatt gggtgctaat ttttcccgta
     1621 tgtcaagcat cttcattcca tattgtttgt tgattgcaat aatcttatca acctgtgcat
     1681 cagataatcc aagctcctgc tttaaacgtt ctacatcacc aaaaatgggg ccctttttca
     1741 tctgcatatc aggacaatca tgtcctcttc ctttaggttc gcccccaaat gctactgatg
     1801 taaccagcat tatcacaaca aacgccagac ttattcgttt catagccatc cccttcatta
     1861 taaaattaaa cgtgaaagct tattcttccc aacaaagcta ttagacctga taaaaacacg
     1921 aaaggttcca taaatggtca tttttctact ataaaattat tgcaattatg ctatctttca
     1981 gtatataggg atatagggtt agcagttcac tacaaataat caaaattttt ttattctttg
     2041 ttatatttta ttatagtgag gtaaaaaatg gttaaccttt cacagccagt actgaaaaca
     2101 atatccagcc gctattcatg ccgtacctat accaatgaag aactaaaaga tgaagactac
     2161 gattatctgc aacagtgtat tgcagcaata agcacattat ctggcactac cctgcgaata
     2221 acgctgatac acaataacag ggtaaagcta ccacaaaaga ttggtacgta tggatttata
     2281 aaaaatcccc agggatttat tgcagcaacc actactgata caaaggaagg gctggtagat
     2341 tttggatatg ctcttgagca gatggtatta gcagcaacag aacgcagtat tggaacatgc
     2401 tggctgggtg gtacctttac cagaggccca tttaaccaga taatgggatt actgccccat
     2461 gagattgtac ctgctgttat atcgctgggc tatccagctg ataaaaaaag gatgtttgaa
     2521 cgctctgtac ggtatatagc tggttctgat acacggaaaa aggctgatga actgttcttt
     2581 gaacatacat ggaacaatcc ctataagggg tcagagcctt ttataacatt gctggaattg
     2641 gtacggcttg cgccatcggc atccaacaaa cagccatggc gtatcctttt caataacaat
     2701 gcattccatt tctatctgga acgtacaccg ggatactacg acaggaacag agccttactg
     2761 ggattccatg accttcagat gatagatatg ggtattgcac tgtgtcattt ccactatgga
     2821 actattgact acaaaataga tggaaggttt gctgtcacac atcatcctgc aatagataaa
     2881 cgcgaatata tcatcagtta tgtattggga taaaatttat gcatacagtg gtatgatgtg
     2941 ctattattat agtaggcaaa atatgtctat tctattttga atagtttaga agttttaatt
     3001 gctgtatttt tcttttttta aatttgctat tttcattgtt atgttgtatt ctttgtataa
     3061 agtttggaca accttacaat agtaaagagg tcacaatgga tacacaatac acatcccgac
     3121 agatactgga aattaaatcc agtgggacat atataatttt atttattttg atagcattga
     3181 tagcgcatgt atttcagttc atagcaaaag ttgatctttt tgaaatcctg aagctttcat
     3241 ttgccagtat aatctgttta cttattgcct atagtatata caaaagaaaa aaaacactta
     3301 aacgaactga aattctggaa tggctattag gctttataac cctgaccatt ccattagctg
     3361 ctaagtttgc gtatgcaaca acctatgact ggacatttgc cctggaatca tacaatagct
     3421 caattttact tattatattc atggttttat tacagcttta ttttaacaaa cgccttgtag
     3481 cgttttattc ttcatatgcc attgttacct gggttgcttt tttattgctg gcgatagctc
     3541 atggggcaga tattcactgg gatgcaacca ttgatgggaa gccaattcac ggctttataa
     3601 tttttagaga aatattcatg atagtaatta ccggcatatt tggaatactg gcgtacaaaa
     3661 acatacagat tgttgaacaa tacgatgaca ggtccaccag gcaactatct attatccatg
     3721 gccaatctga aaagcaaaaa caactgaatg agataatcaa ggaaaaggta tctgatttac
     3781 tgcaacaggt acaacgtcag gatgaattaa ccatcatgtt taatgataag atgcaaaacc
     3841 aggcttcaac ctttgaagag atatcagcaa cacttgaaga gttattaggt tcagccgaga
     3901 gcattgcgca cagctccgaa gagcaggttg gcggcaatac aatgatggaa actattgtaa
     3961 gcgaatttaa gataataaaa aacgaaaccc gtcaaaatct ggataaaacc atacatgaag
     4021 ttgacaatgt tgttgctcag actggcagcg caaatgaacg aatacgggat gtagaatcaa
     4081 ctatcaatga aattcgcaac cagagccaaa aaattgccga aaccattggt gttattgttg
     4141 atatagccga taggattaac cttttatccc ttaatgcaag cattgaagca gcacgtgccg
     4201 gcgattacgg aagaggtttt gccgttgttg ccgatgaaat tggcaaatta gcatatcaaa
     4261 catctgaaag tattaaagac attgagagtg tccttatgca aagcactaaa actaccgaaa
     4321 aaggtgttga agtaattcgt actacggcag caatgctcaa agagctaatt gcaaaaatgg
     4381 cagaaagctc atcaaaaatt aaaattttgc aggacagcac caccgttgaa gaaaaataca
     4441 ttaaaaacat catcgcccag atggaaaaaa atgtggaatt agcaaagaac ataggcacgg
     4501 gcaccgaaga acaaaaggca gctattgaat caagtgccag agcaattgag catatgaatg
     4561 aggttgtcag ccagatggtt gatgaaattc aaaagcttgc aatgacatca cgcagtattc
     4621 ttgacaatgc aaccgctctt cttgaacaat ctaagggcga tagctcatct gagtagcagc
     4681 gtgcctgtta tagagaaact atcaaacaaa caatggaaca aaaatactat cagtacatac
     4741 tattctgtga ttttgatggc actatcactg cagaagaaac atttgtgggg atgtgccaga
     4801 agtttgcacc ggataccttt catgaaattg gcaaaaggat gtgggagcgt cagcttacca
     4861 tcagggaagg tgtacgcgca gttattgagt cattaccttc ttcactatat ccagcgatgc
     4921 ttgcctatat tgatgctatt gacatgaggc ccggtttccc tgagttttta tgttactgca
     4981 ataaccattc tatcccggtt gtcatcatct ctggcggctt atatgattgc gttgttcgta
     5041 agctgggaga tttaatcaat aaagtagagg ctatctatgc tgcaaaggtt gatgcaagcg
     5101 gcgattactt aaagctgtat tctgattatg agtatgaagg tgagttggtg gcaaaagctg
     5161 tcattatgga taactatcgg gcacaatgca ggatagctgt tggcgacgga ccaaccgaca
     5221 tcgagatggc aaaacatgct gatatagtct ttgcgcgtag catgctgaaa cagttttt
//
LOCUS       DAOSOX010000161         5219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_15979_length_5219_cov_7.000775, whole genome shotgun sequence.
ACCESSION   DAOSOX010000161 DAOSOX010000000
VERSION     DAOSOX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15979_length_5219_cov_7.000775"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLX22_10020"
     CDS             <1..689
                     /locus_tag="PLX22_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOT20273.1"
                     /translation="KRRIASITAFVSAFLLYGLSIEPIKDFLVYPLEKQYIASMCIPS
                     LSGKNAYQGDVAKKLSTIDAIVVLGGGVYENGTLKEDSANRMLAGYSTHALTHKTLIL
                     SGGSAIGNVRESNIIYTMLLQLNVNKNKIIIDNTSKDTLENAHNVMKMCNNNGWHRIA
                     LITSAYHMPRAVKLFHTSSLHVEPVPTDSKLDLHYTVYSFMPKFSSLGNSSKAIREYI
                     ALMVLYITGN"
     gene            complement(727..1284)
                     /locus_tag="PLX22_10025"
     CDS             complement(727..1284)
                     /locus_tag="PLX22_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT20274.1"
                     /translation="MDVSFPVNKQSIRLPKINQLGIVVHSIPDAVHYYTTLLGIGPWF
                     RSGTVEHTTLYRGTNISIDVDIVLAFHKGMEVELIELKGGDECIYSECIQRGGGIHHV
                     GSLVLSYDSKMKRVHEAGIQILQSGVIKTRGGATTRYAYLDTVKQCGIITELIETRLY
                     KIPVPQNRVFMEIGCLTGDVGKAHG"
     gene            1437..3839
                     /locus_tag="PLX22_10030"
     CDS             1437..3839
                     /locus_tag="PLX22_10030"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT20275.1"
                     /translation="MSKTKKILIGIAIVIVALVLAIFLFIKNLAKSGAPQYSGQIQLN
                     GLQQPVQVYRDEHGIPHIIAKNEMDVYRATGFVMAQDRMWQMDLLRRVTSGRLSEIFG
                     KDMVDTDVLMRALRMPQKSRFVIAQTDKKVIAHLEAFADGVNQYLEQHKNNLPLEFKI
                     LGYTPESWKVEDSVNLIGYMAWDLTMPWTTETVLYKIWKKVDDAHSKALTPDVKYITD
                     VVFPSFAKNNMPELSNTILPATEKLVSLGAGVFSASNNWAVSGKKSTTGKPILANDMH
                     LGLFIPGIWYQMNQIVEGKLNVTGVAIPGQPCIICGHNEYIAWGMTNVMVDDMDFYLE
                     KINPANKYQYMLNGKWKDMIVVKEEIKIKGEKPVIQEILFTHRGPVVTDFKKMQDVIS
                     MRWMGNEPSNELLTVYMLNYAKNWNDFTNAIRHFRSVSQNIAYADVEGNIGLYSAVGI
                     PIRKGNPMMINPGWTTEYDWQGLVPFERQPHSFNPEEGYIASANNKTVKENYPYYISY
                     WYEMPWRYNRITKLLKQKEKLSVEDFKTIQTDQISELALIWKPLLLHAIERIIEGDSN
                     SLNSYKDVYTLLKDWDGSYSSESIAASVFDTFYIKLVHNIFYDELGDTYDELAKERVL
                     VNLFIDTIRLNNGSIWCDDVTTPQKENLNDMIAKSLNDTIGTLTDSYGTNINKWKWGS
                     MHKLVLKHPLGEVKLLDWLFNYNRGPYPVGGSFHTVCPYSYKLTKPFVITDGASERHI
                     FPMHNLNESLTVIPTGTSGNVASPYYCDQTSLYVGMHYHNDYIDSELIKRTGKYLLQL
                     TP"
     gene            complement(3887..4387)
                     /locus_tag="PLX22_10035"
     CDS             complement(3887..4387)
                     /locus_tag="PLX22_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20276.1"
                     /translation="MVKNYLVRDNDMNLETTTCISYEHLDVIMHHSEKYDMSMRSFIV
                     SFINFVATHKIVPNKPYSRLTYRSRGIAEWKRLHLVLLENEYEFFLDVKKLWKMSLAK
                     IIAFCIENYLYDFIEALENTDNTDNYRFSGYSFGVFLEEGIQCCQFYWGPPPSILKKT
                     KNTLQQ"
     gene            complement(4381..4557)
                     /locus_tag="PLX22_10040"
     CDS             complement(4381..4557)
                     /locus_tag="PLX22_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20277.1"
                     /translation="MPDSGPYLPLKKGGIKSKKSAGLVFNAFTQKHLQLGLKVIEKIQ
                     EKSNEGVMQLRSEW"
     gene            complement(4906..>5219)
                     /locus_tag="PLX22_10045"
     CDS             complement(4906..>5219)
                     /locus_tag="PLX22_10045"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT20278.1"
                     /translation="KISRLPVKGVKIHHLHVIKGTPLAMIYNSGALTLLSCKQYVSYV
                     CDFIERLRDDIIIHRLSGDQLKDMLVAPSWGLHKGTVLKAIEDEFKRRGTYQGFLYDS
                     Y"
BASE COUNT         1656 a         1045 c          985 g         1533 t
ORIGIN      
        1 caaagcggcg aatagcttca attactgcat ttgtttcggc ttttttgctc tacggattat
       61 ctattgaacc gataaaagat tttcttgtat accctttaga aaaacagtat atagcttcca
      121 tgtgcatacc ttccctatcg ggaaagaatg cgtatcaggg tgatgttgct aaaaaacttt
      181 cgactattga tgctatcgtt gtgcttggag gcggtgttta tgaaaatggc acactgaaag
      241 aagattctgc taataggatg cttgccggct atagcaccca tgcgttaaca cacaaaacat
      301 tgatactatc cggagggtca gctattggta atgtcaggga aagtaacatc atatatacca
      361 tgcttttaca actcaatgtc aataaaaata aaatcatcat tgataacacc agcaaagaca
      421 cactggaaaa tgctcataat gttatgaaaa tgtgcaacaa caacgggtgg catcgtatag
      481 cccttattac ctcagcctac catatgccac gagctgttaa actgtttcat acctcatcac
      541 tgcatgttga acctgtaccc accgattcta aattagattt gcactatacg gtttacagct
      601 ttatgccaaa attttcaagc cttgggaatt catcaaaggc tatacgggaa tacattgcgc
      661 tcatggtttt atatataacg gggaattgat aaacaataac tatcgccacc attcaagtat
      721 tttgattcat ccatgggcct ttcccacatc accggttaag catccaattt ccataaatac
      781 cctgttttgt ggaaccggta ttttatatag ccttgtttct ataagttcgg ttataatgcc
      841 gcactgctta acagtatcaa ggtaagcata ccgtgttgtg gcgcctcccc tggtttttat
      901 gacacctgat tgtagtatct gtatccctgc ttcgtgaacg cgtttcattt tgctgtcata
      961 cgatagcacc agagagccta catgatgtat accaccgcca cgttgtatac attcagaata
     1021 aatacactca tcgccacctt tcagctctat aagctctacc tccataccct tgtgaaaagc
     1081 taacacaata tcaacatcaa tggatatgtt cgtacccctg tacagtgttg tatgttcaac
     1141 tgtccctgac ctgaaccatg gaccaatacc cagcaacgta gtatagtaat gaacagcatc
     1201 aggaatagaa tgtactacta tccctaactg attaattttt ggaagacgaa tgctttgttt
     1261 atttacagga aatgacacat ccatatacac ctcaccgaca atgaaaaagc taagagccct
     1321 gtagacgtta ctattgtcaa ataatacccg gtgtggcagt atttagcaaa taaaaaaatt
     1381 ctttactttt attatttcat tcaatagaat gattaacact ataatggagg tttgttatga
     1441 gcaaaacaaa aaagatactt attggcatag ctattgtcat tgttgcttta gtattagcca
     1501 tatttctttt cataaagaat cttgcaaaat caggggcacc ccagtattcg gggcagatac
     1561 agttaaatgg gttgcagcaa ccggtgcagg tgtatcgcga tgaacatggc ataccgcata
     1621 tcattgcaaa aaatgaaatg gatgtttacc gggcaacagg atttgtgatg gcacaggaca
     1681 ggatgtggca gatggatctg ctgaggcgtg ttacatcagg acgactgtca gaaatatttg
     1741 gcaaggacat ggttgatacc gacgtgctta tgcgagcatt acgcatgcct caaaaatcaa
     1801 ggtttgttat tgcacaaact gacaaaaagg ttattgccca tctggaagca tttgctgatg
     1861 gcgttaatca atatcttgaa cagcataaga acaaccttcc ccttgaattt aaaattttag
     1921 gatacacccc cgaaagctgg aaggttgagg atagcgttaa tttaataggc tatatggcat
     1981 gggatctcac catgccctgg accaccgaaa cagtactgta taagatatgg aaaaaggtgg
     2041 acgatgccca tagtaaggct ctgaccccgg atgtaaagta cattaccgat gtggtttttc
     2101 cttcatttgc aaaaaataat atgccggagc tatcaaacac catactgccg gctactgaaa
     2161 aattagtatc actgggcgca ggcgtatttt cagcaagcaa taactgggct gtgtccggta
     2221 aaaagagtac aaccggtaaa cccatccttg ccaatgacat gcatcttggg ctgtttatcc
     2281 ccggtatatg gtatcagatg aaccagatag tagaaggtaa actcaatgta acaggtgttg
     2341 caattccggg acagccgtgc attatatgcg gacacaatga atatattgca tggggcatga
     2401 ccaatgtcat ggtagatgat atggactttt atcttgaaaa gataaacccc gctaacaagt
     2461 accagtatat gctgaatggc aaatggaaag atatgatagt ggtaaaagaa gaaataaaaa
     2521 tcaaaggtga gaagcctgtc atacaggaaa ttttatttac tcaccgcggt cccgttgtaa
     2581 ccgattttaa gaagatgcag gatgtcattt caatgcgctg gatggggaat gaaccaagca
     2641 atgaactgtt aaccgtgtat atgcttaact atgcaaagaa ctggaatgac tttaccaatg
     2701 caatacgcca cttccgttct gtcagccaga acattgccta tgccgatgtt gaaggaaaca
     2761 ttggattata ttcagccgtg ggcatcccta tcaggaaggg caatcccatg atgataaatc
     2821 caggctggac cactgagtat gattggcagg gactggtacc atttgaacgg caacctcata
     2881 gctttaatcc agaagaagga tatatagcat cggcaaataa taaaaccgtt aaggaaaact
     2941 acccctatta tatcagttac tggtatgaaa tgccatggcg ctacaaccgt ataacaaagc
     3001 ttttaaaaca aaaggaaaag ttgtcggttg aagattttaa aaccattcaa accgaccaga
     3061 tatccgaatt agccctgata tggaaacctt tactgttaca tgctattgaa agaattattg
     3121 agggcgatag caactcgtta aacagttata aagatgtata cacgctgttg aaagattggg
     3181 acggcagtta ttcttctgaa agcattgcgg cttcagtttt tgatacattc tacataaagc
     3241 tggtgcacaa tatcttttac gatgagctgg gcgacaccta tgacgagctt gcaaaagaga
     3301 gggtactggt aaaccttttt attgatacca tacggcttaa caacggttct atatggtgcg
     3361 acgatgttac cacaccgcaa aaagaaaacc ttaacgatat gattgcaaaa agcctgaatg
     3421 acactatagg cacactaact gatagctatg gaactaacat caacaagtgg aaatggggca
     3481 gtatgcataa gttagtgtta aaacatccat taggcgaggt aaaactgtta gactggttat
     3541 tcaactataa tcgcgggcca tatcctgttg gaggtagctt ccacaccgtt tgtccatact
     3601 catataagct caccaaaccg tttgtcatta ccgatggtgc ttcggagcgt catatttttc
     3661 caatgcacaa tctcaatgaa tcgcttacgg tcataccaac aggtaccagt ggaaatgtag
     3721 caagtcccta ctattgcgat caaacatcat tatatgttgg gatgcactat cataacgatt
     3781 atattgacag tgagttgata aaacgtaccg gtaaatatct attacagctt accccatagg
     3841 gttattgtag caataatcca gacggtatgc attgatactg tctggattat tgttgtaagg
     3901 tattttttgt ttttttgagt attgatggtg gtggtcccca ataaaattga caacattgta
     3961 taccttcttc aagaaatacc ccaaaagaat aaccgctaaa acgatagtta tcggtattat
     4021 cagtattttc taaagcttct ataaaatcat agagatagtt ctctatgcaa aaagctatta
     4081 tcttagccag tgacatcttc cagagcttct taacatcaag aaaaaattca tactcatttt
     4141 ctaataatac taaatgtaaa cgtttccatt cagcaatccc tcttgatcta tatgttaatc
     4201 gggaataagg tttgttagga acaattttat gtgtagcaac aaagttaata aatgaaacta
     4261 taaatgacct cattgacatg tcatattttt cagaatgatg cattattaca tccagatgct
     4321 cataggatat acaagtcgtt gtttcaagat tcatatcatt atcccttact aaataatttt
     4381 ttaccattcg ctccttaact gcattactcc ctcattgctt ttttcctgta ttttttctat
     4441 aacctttaaa cccaattgca aatgtttttg agtaaaagca ttaaacacta aacctgcact
     4501 ttttttgctt ttaataccac ccttttttaa gggcaaatac ggtccactat ccggcattta
     4561 ctttggctcc cgtcatgaat gcaaagtcgt ctacataacg tcgaaaatta cacagtaaaa
     4621 tgaatcatcc aaaatcatct attgctgaat cggtatagcg ttctaaagct gttcgagcaa
     4681 tggtatccgg gcgtaagttc atgctattat cgtgtgagaa tcacagggta ttgtataatt
     4741 gtataaacgg taaaaatacc ttgccgctga atacatgatt ctaacaatag ataccaacca
     4801 tcatgttacc tctatcaaag tttttgcaat tatacatgaa gtaacataca agttttattg
     4861 ttactatcat aggcgaatat tgtttataat gcaatcacaa aattttcagt aactatcgta
     4921 caaaaaaccc tggtaagtac cacgcctttt gaattcatcc tcaattgctt taaggactgt
     4981 ccctttatgc aaaccccatg acggtgccac cagcatgtct ttcaactgat ctcctgataa
     5041 cctgtgaatg ataatatcat cacggagccg ctcaataaaa tcgcatacgt atgaaacata
     5101 ctgtttgcat gacaaaaggg taagtgcacc gctgttataa atcattgcca acggtgtgcc
     5161 ttttataaca tggagatgat gaatctttac cccttttacc ggaagcctgc ttattttat
//
LOCUS       DAOSOX010000162         5201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16055_length_5201_cov_10.306452, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000162 DAOSOX010000000
VERSION     DAOSOX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16055_length_5201_cov_10.306452"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLX22_10050"
     CDS             complement(<1..1139)
                     /locus_tag="PLX22_10050"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOT20279.1"
                     /translation="MKRITTTSFFVIFIVLSSAVFPLDNNVVIFQKAKEDVMKGMYHF
                     NEMHYLAAIEFFRKALEVYPDYYTASEYLARSYKLAGYTDLALQQWQLLLEMTKGNVL
                     VQNKIDMINFRSSLYNGLEKSFELIEAAEYNPKELGRFRFSVPIDIAIDNDKNMYITS
                     FANGKLVKLDPNGQGIFTRTYSLEGKLYGIDYKSGMLAVSDFANNTVLIINTDGRVVK
                     KIGSTGNKELQFNGPEGVCFGGDSSLYVVDSGNHRVQKFGVDGRFILAFGQYGEYEGQ
                     LNKPTDVAVRNENVYVTDTNNKRIAVFDDSGNFVENMTPDEFAVPRGIYIKNNLLTIA
                     DEKKGLFMYNFENENHQWFASWEGKKKFYHLTSAVVDDNGFIYAC"
     gene            complement(1227..>1460)
                     /locus_tag="PLX22_10055"
     CDS             complement(1227..>1460)
                     /locus_tag="PLX22_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20280.1"
                     /translation="KLIDVLQSLVDKGNTVIVIEHNLDVIKCADWIIDLGPEGGDAGG
                     RIVAEGSPEDIAKIKQSYTGQFLKKIVLHKKIN"
     assembly_gap    1461..1560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1561..3909)
                     /gene="uvrA"
                     /locus_tag="PLX22_10060"
     CDS             complement(<1561..3909)
                     /gene="uvrA"
                     /locus_tag="PLX22_10060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT20281.1"
                     /translation="MSQQFIRIKGAREHNLKNISVDIPRERLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIHDYLRLLFARVGIAYCYQCGKPISSQTIDQIVENVLTHPQGTKVQVLAPVVRGR
                     KGMYQDLFEKMKQSGYSRVRVDGQLHLLDDPIAIDKNKKHSIDVIIDRITLKENIRAR
                     LSDSIETAIQLAGGQVVLIINDNEQLISTKMTCPDCDISLPELSPRMFSFNSPYGACP
                     SCDGLGFIMQFDPDLIVDNPDKSLMDGALAVWGKTTNYWYQEQVVALARHLKFDPYTP
                     WKKLSRSIQQVVLYGSNGKKIDYSIERHEAQYTFAKAFEGVISNLTRRYKETQSDEMR
                     EWMERFMRNRVCDACGGRRLRPESLAVKVADKNIADISALSIKDAVTFFHELSLSETQ
                     MAIARQIVKEITARLLFLSNVGIDYLTLDRAAGTLSGGEAQRIRLATQIGSGLTGVLY
                     ILDEPSIGLHQRDNKKLLDTLKELRDIGNTVIVVEHDEETIRESDYVIDLGPGAGLDG
                     GYIIAQGTPEEIENNESSITGLYLNGKRFIAQPQTRRKSEHHLVIRGASENNLKAIDV
                     SFPLGVFTCITGVSGSGKSTLIIDILYKALASLKMHSKDYPGKFKKIEGIEYIDKVID
                     VDQSPIGRTPRSNPATYTGTFTPIRELFANLPESKIRGYKPGRFSFNVSGGRCEACWG
                     DGIKKIEMHFLPDVYITCEVCKGKRYNHETLEVRYKGKNIYEV"
     gene            complement(3906..4193)
                     /locus_tag="PLX22_10065"
     CDS             complement(3906..4193)
                     /locus_tag="PLX22_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20282.1"
                     /translation="MANEFEKEFDDEVYEDDFYEESFDDEELLEEESIEDDAQDEQEE
                     IEEDEKHIAVILACDDCDYRWEDVIVKRKDEDIEDLEIICPMCGSTNVTQI"
     gene            complement(4498..>5201)
                     /locus_tag="PLX22_10070"
     CDS             complement(4498..>5201)
                     /locus_tag="PLX22_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20283.1"
                     /translation="NILHVKNFLPSMYYHTIIVTMKKYTITLYTIIFLLICSASYSQQ
                     LSPIVKLYPFNAADTYLINWKWITALDYKKISEWAKIYRCSVNYDDYPIIDGVAWFVS
                     KYGSTIIFDNLDDIKYFLHINFVTFDDPEKTGIDALCIVSCNGKPVKRLAFSDMIVNN
                     SRVTIEITREYIVNKTMLVEFKEYSSAGGFFGVWDVVLSKSDVLPQIIDIPESKSSKS
                     KKGTMETIPQTLIKP"
BASE COUNT         1518 a         1141 c          832 g         1610 t
ORIGIN      
        1 ctgcacgcat aaataaatcc gttatcatcc acaacggctg atgttaaatg ataaaatttc
       61 tttttgcctt cccagcttgc aaaccactgg tgattttcat tttcaaagtt atacataaaa
      121 agcccttttt tctcatcagc aattgttaaa agattgttct ttatatatat accgcgagga
      181 acggcaaact cgtcaggggt catattttcc acaaaatttc ccgaatcatc aaacacggct
      241 atgcgcttat tgtttgtatc ggttacatac acattttcat tacgcacagc aacatcggtg
      301 ggtttattaa gctgtccttc gtactcacca tattgtccaa aagcaaggat aaatcgtcca
      361 tcaacgccaa acttctgcac acggtggttg ccagaatcaa ccacatacag ggaactatcg
      421 ccaccaaaac aaacaccttc aggcccgtta aactgcaact ctttatttcc cgttgagcca
      481 atcttcttta ctaccctgcc atcggtatta ataattaaaa ctgtattgtt tgcaaaatcg
      541 ctgacagcaa gcatcccgct tttataatca atgccatata atttgccttc caggctatag
      601 gtgcgggtaa agattccctg cccgttggga tcaagcttca caagctttcc attggcaaat
      661 gaggttatgt acatgttttt gtcattatca atagcaatat caatgggcac cgaaaacctg
      721 aatcgtccaa gctcttttgg attatactca gcagcttcta tcagttcaaa tgacttctca
      781 agcccattgt acagtgagct tctgaaatta atcatatcta tcttgttctg gacaagtaca
      841 ttgccttttg tcatctccag cagcaactgc cactgctgga gagctaaatc agtatatcct
      901 gccaatttat atgaacgcgc cagatattcg ctggcggtat aatagtcggg ataaacctct
      961 aacgcttttc ggaaaaactc aatggcagca aggtagtgca tttcattgaa atggtacatg
     1021 cctttcataa catcttcttt ggctttctgg aatatcacca cattgttatc taatgggaaa
     1081 actgccgaag aaagtacaat gaatataaca aaaaaactcg ttgttgttat tcgtttcaca
     1141 gtattactcc atggaatata ataatgcacc cctttatgag tatcggtttt atgtacaggg
     1201 taagtgtata gtgtaaatta ttgcaattaa tttatctttt tgtgtaatac tatcttcttt
     1261 aaaaattgtc ctgtatagga ttgctttatc tttgctatgt cctcagggct tccctctgcc
     1321 acaatcctgc cgccggcatc accgccttca ggtcccagat caattatcca gtcggcacat
     1381 ttgatgacat ccagattatg ttcaataact atcaccgtat tgcctttatc aactaaactt
     1441 tgcaatacat caataagctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 cacttcatag atatttttac ctttatagcg cacctccagt gtttcatgat tgtagcgttt
     1621 gcctttgcac acctcacacg ttatatatac atcaggtaaa aaatgcatct caatcttctt
     1681 tatgccgtca ccccagcacg cctcacatcg cccgccactc acgttaaacg aaaaacgtcc
     1741 cggcttatag ccgcgtattt tagattcggg caaattggca aacagctccc gtatgggagt
     1801 aaatgtaccg gtataggtag cagggttgga gcgcggcgtc ctgccaatgg gcgactgatc
     1861 aacatcaatg actttatcga tatattcaat cccttcaatt tttttaaatt taccgggata
     1921 atctttgctg tgcatcttta acgatgccag tgctttatac aaaatgtcaa taatcaaggt
     1981 tgatttgccc gaaccggata caccggtaat acaggtaaac actcctaaag gaaatgaaac
     2041 atcaatagct tttagattat tttcagaagc gccacgtatc actaggtggt gctcgctttt
     2101 tctacgggtt tgaggttgtg caataaatcg cttgccgtta agatataacc ccgttattga
     2161 gctttcatta ttttcaatct cttccggtgt cccctgtgca atgatatatc caccatcaag
     2221 ccctgcacca ggtcctaaat caatgacgta atccgattcg cgaatggttt cttcgtcatg
     2281 ttcaacaact attaccgtat tgcctatatc cctcagttcc tttaatgtat ccagaagttt
     2341 tttgttatca cgctgatgca atccaatgct tggttcatcc agaatataca gcaccccggt
     2401 taaccccgaa cctatttgtg tggcaaggcg tatacgctgt gcctcaccgc ctgaaagtgt
     2461 cccggcagct cggtcaagcg tcagataatc aataccaaca tttgaaagga ataaaagcct
     2521 tgccgtaatc tccttcacta tctgacgggc aattgccatc tgcgtttctg aaagcgaaag
     2581 ctcatgaaaa aatgttactg catccttgat tgacagcgca ctgatatcag caatattttt
     2641 atcagcaacc tttactgcca gactttccgg acgcaaacgc ctgcctccgc atgcatcgca
     2701 tacacgattg cgcataaatc gttccatcca ctcgcgcatc tcatccgact gcgtttcttt
     2761 gtagcgacgg gttaaattgg aaataacccc ttcaaatgct tttgcaaatg tatactgcgc
     2821 ttcatgacgt tcaatggagt agtcaatttt tttaccattt gatccataga gtacaacctg
     2881 ctgaatactt ctggaaagtt ttttccatgg ggtataagga tcaaacttta aatgacgggc
     2941 aagcgccact acctgttcct gataccagta gtttgttgtt tttccccata ccgctaatgc
     3001 accatccatc aacgatttat caggattatc cacaatcaaa tcgggatcaa actgcataat
     3061 aaagccaagc ccatcacatg acggacaggc gccatacgga ctattaaatg aaaacattct
     3121 tggcgatagt tccggtaatg aaatatcaca gtcaggacag gtcatttttg ttgaaataag
     3181 ttgctcgtta tcattgatga ttaatacaac ctgccctccg gctaactgga tagcagtttc
     3241 aatggaatct gataaccgcg ctctaatatt ttctttcagt gtgatacgat caatgatgac
     3301 atctattgaa tgcttcttgt ttttatcaat ggcaatagga tcatccagca aatgcaactg
     3361 cccatccacc ctgacccgtg agtatcccga ttgtttcatc ttttcaaaca aatcctgata
     3421 catccctttt ctgcccctga ccaccggtgc cagcacctgc acctttgttc cctgcggatg
     3481 ggttaacaca ttttcaacaa tctggtcaat ggtttgcgaa gaaatgggtt tgccgcattg
     3541 gtaacagtac gcaataccaa cacgggcaaa taataaacgc aggtaatcat gaatttcagt
     3601 aacagtacca acggttgaac ggggattacg atgagttgtt ttttgttcta ttgatatagc
     3661 aggcgatagt ccttcaatga ggtcaacatc cggtttttcc atctgtccta aaaactgccg
     3721 cgcataggct gacagcgact caacatacct gcgctggcct tctgcataaa ttgtatcaaa
     3781 agccagagat gatttccctg aaccggaaag accagtaata accaccagcc tttcacgcgg
     3841 aatatcaaca gatatatttt ttaaattgtg ctcacgggca cctttaatac gaataaattg
     3901 ctgactcata tctgggttac attagtagaa ccgcacatag gacatataat ctctaaatcc
     3961 tcaatatcct catcttttct ttttacaata acgtcttccc atcgataatc acaatcatca
     4021 caggccaaaa ttaccgctat atgtttttca tcttcttcaa tctcttcctg ttcatcctga
     4081 gcatcatcct ctatactttc ttcttcaagt aattcctcat catcaaaact ctcttcatag
     4141 aagtcgtctt catatacttc atcatcaaac tctttttcaa attcatttgc catacgctat
     4201 cacccttttt aagcagtatt ttactataaa atactattaa caatatatga attcttaaaa
     4261 aactatctaa caaaaaaatg aaaaaataaa aaatttttat cacaaaggtg acaaaaccaa
     4321 tactgtcgtt aaaatttcac aatagcaatt ttcatattcc tttttgtcac aaaagttagt
     4381 atcgttgaaa tggtcaataa aatttcagta ataattaaaa aaaaattggt ttgctacact
     4441 gaaaaattgt aatttactac aaaatataca aaacactatt ttttttgttt tttagcttta
     4501 aggctttatc agtgtttgtg gaattgtttc catagttcct tttttacttt ttgaactttt
     4561 tgattctgga atatctataa tctgaggaag cacatcgctt ttgcttaaaa caacatccca
     4621 cacaccaaaa aaaccgccag cagaagaata ttctttaaac tcaacaagca ttgttttgtt
     4681 aacaatatat tcccgtgtta tttcaatggt taccctgctg ttattgacaa tcatatcact
     4741 gaaggctaat cttttcacag gtttaccatt acatgataca atacacagtg catcaatccc
     4801 ggttttttct ggatcatcaa atgttacaaa atttatatga agaaaatatt ttatatcatc
     4861 aagattatca aaaatgatgg ttgacccata ttttgataca aaccatgcaa ccccgtctat
     4921 gatgggataa tcatcgtagt ttacactgca gcgatagatt tttgcccact ccgaaatttt
     4981 tttataatca agagcggtta tccacttcca gtttattaaa taagtatcag cagcattaaa
     5041 tgggtacaat tttactatgg gcgatagctg ctgtgaatat gatgcagaac atataagcag
     5101 gaaaattata gtatataatg ttatagtata ctttttcatt gttacaatta ttgtatgata
     5161 atacatagaa ggcaagaaat tttttacatg caaaatattt t
//
LOCUS       DAOSOX010000163         5174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16175_length_5174_cov_7.353780, whole genome shotgun sequence.
ACCESSION   DAOSOX010000163 DAOSOX010000000
VERSION     DAOSOX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16175_length_5174_cov_7.353780"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLX22_10075"
     CDS             <1..531
                     /locus_tag="PLX22_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOT20284.1"
                     /translation="HKTEVDGVRLNIMDVEQLEDAFIDMKQSLYDYNPNAVFKGIYVH
                     TMAKAGVEFIVGAQKDAVFGPIVMVGLGGIYVELFKDIAIRLAPVTKVEAWDMIQQLK
                     SAQLFEGFRGKEPLDAEALSEVIMTLSYAISAIDEIADIECNPVMVYPKGEGCIAVDA
                     RMVLQKQTIVSKATTS"
     gene            646..2028
                     /locus_tag="PLX22_10080"
     CDS             646..2028
                     /locus_tag="PLX22_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT20285.1"
                     /translation="MQRTVETLPLWKKIIYALGQLGWSLASFSVGNLLVYFYLPPEES
                     NNAIFQPYIYQGYVIGVLTIIGLIYAFGRLWDAITDPIIATMSDRSKSRFGRRRKFLA
                     IGGLPFALLSILAFTPPVNGMSPWNAVWLFVVVTLFYLFMTMYVTPFFAWLSELGHNP
                     RERLFLSTLISITWAIGFAIGSQAVALQSMFEKSYGYSSHRAFQTVMVIFGGISLILM
                     YLPVLFIDERRYCESNVSTEGTFEALRSAFKNKNFLFFTISDLAYWVALTFITTGLVY
                     YVTVLLQMDKAVYSQYMIILFALSFIFYVPVNVIAQKIGKKKLMIIAFILFSMGYVIA
                     SMLGWFPFDKNIQGLIVIIITSLPLAIFGILPNAIIADIAEADGIEKGNFKAGIFFGA
                     RTFMSKMGQMIAALVFPSLILLGKTPENDIGVRLTCIAACFFCIAGLLLFMKYDEKKI
                     ISILAKKEKV"
     gene            2405..2869
                     /locus_tag="PLX22_10085"
     CDS             2405..2869
                     /locus_tag="PLX22_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20286.1"
                     /translation="MIKVTISRTFSFPIDEVYSAIDHINKLARLKPAFRNYRIARDEE
                     GLQLIDVDIRFIVNKFPMRLTYTAIPFKYCELKKLKGAIKEYSYTYTFEQSDNKTSVT
                     ADCAIVLPLRYFIVQPFLSAIIKHRLAKELTILEKIVNQKFNGYDKTTYRSE"
     gene            2841..4523
                     /locus_tag="PLX22_10090"
     CDS             2841..4523
                     /locus_tag="PLX22_10090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT20287.1"
                     /translation="MIKPLIVQSDNTLLLEVDNPLYEEVRDAILPFAELEKSPEHIHT
                     YRITPLSLWNAASSGLTTKEILERIAQYSRYDVPSIVVTTIKEQMQRYGLLTLIKHNE
                     NCILQSNDAHIIREIVTHKSIQKFIDSQLSDYELVIKPYSRGHIKQALIKIGFPVEDK
                     AGYIDGDPCPFALRTVTQSGSPFVIRDYQQNAINSFYRHGSDEGGSGVVVLPCGAGKT
                     IVGMGIMHIYQTKTLILVTNTVALRQWIDELLDKTTLSTDMIGEYSGDVKDIKPVTIA
                     TYNILTYRRKKEDEFKHFKLFTENNWGLIIYDEVHLLPAPVFRMTAEIQSKRRLGLTA
                     TLVREDGLETDVFSLIGPKKFDMPWKMLEECNWIAKAICSEIRIELPESIRYQYTVAG
                     DRQKFRIASENEKKLYYIERLLNAHPNDRVLIIGQYIDQLQIIKERFNYPIITGSTPV
                     KEREELYKQFKGGTITRLIVSKVANFSIDLPDANVAIQVSGTFGSRQEEAQRLGRILR
                     PKPGDNTAYFYSIITAHTVEEHFAHNRQLFLTEQGYTYIIMNESMFHEKFSA"
     gene            4504..>5174
                     /locus_tag="PLX22_10095"
     CDS             4504..>5174
                     /locus_tag="PLX22_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20288.1"
                     /translation="MKSLALSLILVFSSTVSSWKPLLVQNNGFLYYHEAKCKGLPANI
                     VINDIQVHGNIIYLTTSQHGIFKNNGGDSYWENITPASAYTRTIHSPCNQYRSISAFC
                     VDGCDPARLYCATKYTLYTSGDAGKRWEVIPMTGLPANACITALYAKGGQLVVGTSWN
                     GIYRYTAGRFVAVNGSFPRKMYSDSIGFYETIAHVSGGSPVRAATRFTPQVYSYGASG
                     WKMVFA"
BASE COUNT         1586 a          900 c         1124 g         1564 t
ORIGIN      
        1 cataaaacgg aggttgatgg agtcaggctc aatataatgg atgttgaaca gttggaagat
       61 gcttttatag atatgaaaca gtcactgtat gattataacc ccaatgcagt atttaaaggc
      121 atatatgtgc atacaatggc aaaagcaggc gttgagttta ttgtaggagc acaaaaggat
      181 gcagtctttg gtcccattgt aatggttgga ttagggggga tatatgttga actttttaaa
      241 gatatagcta tacggttagc cccggttaca aaagttgaag catgggatat gatacaacag
      301 ttgaaatctg cacaattgtt tgaaggcttc agaggtaaag aacctcttga tgcagaagca
      361 ttaagtgaag tgattatgac cttgagctat gctatttcag ctattgatga aattgctgat
      421 attgaatgca accctgttat ggtgtatccc aaaggtgaag gttgtattgc tgttgatgcg
      481 cgtatggttt tacaaaaaca aacaattgtt tcaaaagcta ccaccagtta aaatggaaat
      541 cacagtttta aatgtggtgg gtgctatgag aaaaaataat tgcaatttag tatatatatc
      601 ataataatgt cgatatcgat ataataagca gtgtgaggtg gtatcatgca acgtactgtt
      661 gaaacacttc cattatggaa aaagattatc tatgcattag gccagctggg atggtcactt
      721 gcttcattta gtgttgggaa ccttctggta tatttttacc tgccaccaga ggaaagtaat
      781 aatgcaattt tccagcccta catttatcag gggtatgtta taggtgtttt gactattatt
      841 ggattgatct atgcgtttgg caggctgtgg gatgctatta ctgatcctat tattgcaaca
      901 atgtcggaca ggtcaaaatc aaggtttgga aggcgcagaa aatttttggc aattggtgga
      961 ctcccttttg ctcttttgag tatattggca tttacccctc cggttaatgg tatgtcacca
     1021 tggaacgcgg tatggctttt tgttgtggtg acactttttt acttgttcat gacaatgtat
     1081 gtaacaccat tttttgcatg gttatctgag ttgggccata atccccgtga acggcttttt
     1141 ttaagtacgc tcatttcaat tacatgggct ataggatttg caataggttc acaggctgtg
     1201 gcgctgcaat caatgtttga aaaaagctat ggatatagtt ctcaccgagc cttccagaca
     1261 gtgatggtta tatttggtgg aatatcacta atcctcatgt atctgccagt attatttatt
     1321 gatgaacgca ggtattgtga atcaaatgtt tcaactgagg gaacgtttga agcattacgt
     1381 tcagctttta aaaataaaaa ctttttgttc ttcaccattt ctgatttagc atattgggta
     1441 gcgttaacat ttattactac tggacttgta tactacgtaa cagtgctttt acaaatggat
     1501 aaagcagtat attcacagta tatgattatt ctttttgctt taagttttat attttacgta
     1561 ccggtcaatg taattgccca aaagattggt aaaaaaaagc ttatgataat tgcatttatt
     1621 cttttttcca tgggatatgt tattgcctca atgctgggat ggtttccctt tgataaaaat
     1681 atccagggat tgatagttat tatcattaca tcattaccgc tggcaatctt tgggatattg
     1741 cccaatgcaa ttattgctga tattgctgaa gcagatggaa tagaaaaagg taattttaaa
     1801 gccgggatat tttttggagc cagaacgttt atgtcaaaaa tgggacagat gatagcagct
     1861 ctggtgttcc cttcattaat actgttgggt aagacaccag agaatgatat aggagtacgg
     1921 ttaacctgta ttgcagcatg tttcttttgt attgccgggt tgttattgtt tatgaaatat
     1981 gatgaaaaga aaataataag catattggca aaaaaagaga aggtttaaga taacattcat
     2041 caccagggat gaccagtctc atccggtttt agcatcagtc aatccatgaa ggatggaaat
     2101 gtggatgttg acagttatcc tgaacggctg attcagtatc taattatttt gattttgggc
     2161 caagctcaga atctcgttgt tttatgaata aataggttct tcgggactgc gtccctcaaa
     2221 atgacaggca tgcagggtgt cgtcctgaat tccgattcag gatctatcat gacagggggt
     2281 gtataattct attttcgcgg gagatagtat tgttttgtgg tttgaaacag cacaattaag
     2341 tatttgacaa atttttcata ggatgcagaa tacatggtgc agtttttggt aaaagagatg
     2401 tatcatgata aaagtaacca tttccagaac attttcattc cccattgatg aggtttattc
     2461 agcgatagat catatcaata aattggcacg gttaaaaccc gctttcagga actatcgcat
     2521 agctcgtgac gaagaaggat tacagcttat tgatgttgat attcgtttta ttgtaaataa
     2581 atttccaatg aggcttacct atactgcgat accttttaaa tactgtgagc taaaaaagtt
     2641 aaaaggggct attaaggaat atagttatac ctatacattt gaacaatctg acaataaaac
     2701 cagtgtaaca gcagattgtg ctatagtact ccctttacgg tattttattg ttcagccatt
     2761 tctaagtgct ataataaaac acagacttgc taaagaactc acaattttag aaaaaatagt
     2821 aaaccaaaaa tttaacggat atgataaaac cacttatcgt tcagagtgat aatactctgc
     2881 ttctggaagt agacaatcca ctatatgaag aggtgcggga tgctattctt ccttttgctg
     2941 aacttgaaaa aagtcctgaa catatacata catacaggat cacaccatta tccctgtgga
     3001 atgcagcatc gtcgggatta actacaaaag aaatacttga gcgcattgca cagtattccc
     3061 ggtatgatgt gccatcaatt gttgtcacca ccattaaaga acagatgcag cggtatggac
     3121 ttttgacatt aataaaacac aatgaaaatt gtatattgca aagtaacgat gctcatatca
     3181 ttcgtgagat agttacccat aaatccatac aaaagtttat tgatagtcaa ctgtccgact
     3241 atgaactggt aataaaacca tacagtcgtg gacatataaa acaggctctt ataaaaatag
     3301 gatttcctgt tgaggataaa gccggctata ttgatggcga cccctgtcca tttgcattgc
     3361 gaacggttac gcaaagtggt agtccgtttg ttatccgtga ttatcaacaa aacgcaataa
     3421 acagctttta taggcatggc agtgatgaag ggggaagcgg tgttgttgtg cttccctgtg
     3481 gtgcggggaa aaccattgtt gggatgggca tcatgcatat ctatcaaaca aaaacactta
     3541 tcctggtaac caacaccgtt gcattgcgtc agtggattga tgaactgctg gataagacaa
     3601 cgctgtcaac tgacatgatt ggtgagtatt ccggtgatgt taaggatata aaaccggtaa
     3661 ctattgccac ctataacata ttaacctacc gtcgaaaaaa agaagatgaa tttaaacatt
     3721 ttaaactgtt taccgaaaac aactgggggc ttattatata cgatgaggtg catctactgc
     3781 cagctccagt gttcagaatg actgccgaga ttcaatcaaa acgcaggtta gggcttactg
     3841 caacactggt gcgtgaggat ggacttgaaa cagatgtatt tagcctcatt ggaccaaaaa
     3901 aatttgatat gccatggaag atgctggaag aatgcaactg gattgcaaag gcaatctgtt
     3961 ctgagatacg cattgaactg ccggaaagca tacgctatca atacaccgtg gcaggcgaca
     4021 ggcaaaagtt tagaatagca tcagaaaacg aaaaaaaact atactacatt gaaaggctcc
     4081 tcaatgcaca tcccaatgac agggtattga ttattggaca atatattgat cagttgcaaa
     4141 taatcaagga gcgttttaac tatcctatca taaccggaag tacaccggta aaggagcgcg
     4201 aagagctcta caaacaattt aaaggtggta ctattacacg gctcattgta tcaaaggttg
     4261 caaatttttc tattgacctg ccggatgcaa acgttgccat tcaggtgtca ggcacatttg
     4321 gttcaaggca ggaagaggca caacggctgg ggaggatttt gcgcccaaag ccaggtgata
     4381 ataccgctta cttttattca attattaccg ctcatacggt tgaggaacat tttgctcata
     4441 atcgccagct ttttttaacc gagcagggat acacctatat catcatgaat gagagcatgt
     4501 tccatgaaaa gtttagcgct tagcctcatt cttgtttttt cttcaacggt atcctcatgg
     4561 aagccattgc tggtacaaaa caatggcttt ttatattatc atgaagcaaa gtgtaaagga
     4621 ttaccagcaa acattgtcat caatgatata caggttcacg ggaacatcat ataccttaca
     4681 acatcgcagc atggtatttt taaaaataat ggtggcgata gttactggga aaatataacc
     4741 cctgcaagcg catatacacg caccatacat tcaccgtgta atcagtatcg aagcatatca
     4801 gcattttgtg ttgatggctg tgaccccgcc cggctgtact gcgcaacaaa atatacgctg
     4861 tatacatcgg gtgatgcggg caaacgctgg gaggttatac caatgacagg gctgcctgct
     4921 aatgcatgca ttactgcgct ctatgcaaag ggcggtcagc ttgttgtggg gacatcgtgg
     4981 aatggcatat accgttatac ggcaggcaga tttgttgcag taaacggcag ttttcccagg
     5041 aagatgtatt cggacagcat tggcttttat gaaacaattg cgcatgtttc cggtggctcg
     5101 ccggtacggg cggcaacacg ctttacaccg caggtgtatt catacggtgc gtcaggctgg
     5161 aagatggtgt ttgc
//
LOCUS       DAOSOX010000164         5146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16299_length_5146_cov_8.654685, whole genome shotgun sequence.
ACCESSION   DAOSOX010000164 DAOSOX010000000
VERSION     DAOSOX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16299_length_5146_cov_8.654685"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLX22_10100"
     CDS             <1..201
                     /locus_tag="PLX22_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOT20289.1"
                     /translation="DISIKNTDEIILLCNKYSVKVIGKIPLDTDFVKAQIAGKTIVEF
                     SKGNASQAIQEIWDSLQVKLFS"
     gene            258..1961
                     /locus_tag="PLX22_10105"
     CDS             258..1961
                     /locus_tag="PLX22_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT20290.1"
                     /translation="MLGKKLRVVIVGGVACGPKTASRIKRLLPDAEVTIIEKGELLSY
                     AGCGLPFYLSGEVHDYKELMSTAAGVVRDTHFFKMTKDITVLNQTLVESIDRNKKTIK
                     AVHTLSGKSIDIEYDKLVLAVGGTPFIPQIKGIDLKGVHFLSTIDDARKIHDEEKNLK
                     GKNAVIIGGGLIGIEVCESFVKQGMNVTIIEKMDRVLPALLDPEMAYHVHNELQANNV
                     TVLLNDAVVEIKGDDQGNVRSVKTNKTEVPSDLVLIAIGTKPNITLAKQAGITIGTTG
                     AIAVNEFLCTSDPDIYAGGDCVENIHLVSGKPIYAPMGSTANKHGRVIANNICGKEST
                     FKGVLGTAICKVFTMNVARTGLSEEQARDGGYDFITVLNPSQDKAHFYPDAKLIIIKL
                     IVDKKSRTLLGAQIVGPGDVAKRIEVVITTITNKATIDDIANYDLAYAPPFSPAIDNI
                     IMTAYIALNKLDGITKSISPVEVKKKMGNNEDFILLDVRSPQEYEEIRIDDVRVKLIP
                     LGKLRESLHQLDKSKEIITFCKISLRGYEAQRILEGAGFRNVKFLDGGIVCWPYKKLV
                     K"
     gene            1979..2860
                     /locus_tag="PLX22_10110"
     CDS             1979..2860
                     /locus_tag="PLX22_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOT20291.1"
                     /translation="MMFGNNKNSKSNAVKPGMNDKQAIEVDEELKLEENLNKIKHTIL
                     VLSGKGGVGKSTIATNCAVALASRKLKTGLLDIDIHGPSVPTLLGIEKLGLQATNHNT
                     IIPIQYNDYLKVISAGFMLPDNKEAVIWRGPLKYNLIKQFLSDVEWDELDFLIIDSPP
                     GTGDEPLSVAQLVKNPTGAVIVTTPQNVSTIDVKKSITFCHKLNIPVLGVVENMSGFV
                     CPHCGKETFIFRKSGGSEISQEMGVPFLGSIPIDPLIVENSDAGRPFAQSANSNATAS
                     QKHFEEIISSLLQSITV"
     gene            2875..3216
                     /locus_tag="PLX22_10115"
     CDS             2875..3216
                     /locus_tag="PLX22_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT20292.1"
                     /translation="MLIAVPLTQDNIFSLHFGHCNKFAMYDIDENTKSIVKKEEIPAP
                     PHQPGLLPRWLSEKGATCILAGGIGQRAMSLFEEQHVHVVIGIEGTDPDAIVNDYLAG
                     KLKVGANVCDH"
     gene            3229..3522
                     /locus_tag="PLX22_10120"
     CDS             3229..3522
                     /locus_tag="PLX22_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20293.1"
                     /translation="MSTVCITLYLNGHCIESEAKNQLHRLYELILNSYNVTSELENQY
                     KLLYDFITIADFKALRSMDERLNGLMPSICELTKDDEGKIQLQIQGCNLFFNF"
     gene            3903..4961
                     /locus_tag="PLX22_10125"
     CDS             3903..4961
                     /locus_tag="PLX22_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT20294.1"
                     /translation="MSDIYSALQKRLDMYSLGFPETQSKIEITILKKLFSEDDAKLFM
                     ELSPMLEPAESIAARMGKPVQEIQNHLEDMVKRGLLFKKTKDGVAKYGAIPFIHGIFE
                     FQVKRLPKDLAELLEQYYDDGLAKAIATNANMFLHPIPVNESIESKSTVAPFEDAVAI
                     IQQANGIAVADCICRKEKKTLGKGCDKPMETCLMFGSMAEYYIENNLGRKISADEAIA
                     IVKNAQKQGMVTQPGTAQNPAGMCSCCGDCCAVLLSIKRFPKPAEMVFSNYFAEVNDN
                     CTSCGSCADICQMDAIEMNDFAYVNKDRCIGCGLCVSECPADAIHLVLKDNKRIPPKT
                     SGEQMMQMAKQRGILPDG"
BASE COUNT         1783 a          829 c         1008 g         1526 t
ORIGIN      
        1 gatatatcaa taaaaaatac tgatgaaatt atattgttgt gcaataaata ttcagtaaaa
       61 gtaattggta aaattccgct tgatactgat tttgtgaagg cacagatagc aggtaaaaca
      121 attgttgaat tttctaaggg caatgcttca caagctattc aggagatatg ggattcttta
      181 caggtaaaat tgttttcata aaaaaaggaa tatcaacacc ctaacaggta cattaaatac
      241 taaaaggagt ttatataatg ctgggaaaaa aattaagagt tgttattgtt ggtggagttg
      301 cctgcggacc aaaaacagct tcacgaatta agcgactgct tcctgatgca gaggtaacaa
      361 tcattgaaaa aggcgaactt ttatcgtatg ccgggtgcgg attgcctttt tatctttcag
      421 gcgaggtgca tgattataaa gaactgatga gcacagcagc aggggttgtg agggatactc
      481 atttttttaa aatgacgaaa gatattaccg ttttgaatca aacgctggtt gaatcaattg
      541 accgaaacaa aaaaacaata aaagcggtac atacgctatc aggtaaatcc atagatattg
      601 aatatgacaa attagtgtta gccgtggggg gcacaccatt tataccccaa attaaaggta
      661 ttgatttaaa aggagtacat ttcctttcaa ctattgacga tgcacgaaaa attcatgatg
      721 aggaaaaaaa tttaaaaggc aaaaatgccg ttatcattgg aggtggattg ataggaattg
      781 aagtttgcga atcttttgtt aaacagggga tgaatgtaac cattattgaa aaaatggata
      841 gggtgctccc tgcacttctt gatcctgaaa tggcttatca cgttcataat gaattacaag
      901 ccaacaatgt tactgtatta cttaacgatg cagtagtaga aattaagggc gatgatcagg
      961 gtaatgtaag aagcgtaaaa acaaataaaa cggaagtgcc ctcggatttg gtattgattg
     1021 ccattggtac aaaaccaaat ataaccttag caaaacaagc tggcattact ataggtacaa
     1081 caggtgccat tgctgttaat gaatttttat gtacatctga tcctgatatt tacgctggtg
     1141 gtgattgtgt tgagaatata catttggttt cagggaaacc aatctatgct cctatgggtt
     1201 caacagccaa taaacatggg agagttattg caaacaatat ttgtggaaaa gaatcaacgt
     1261 ttaaaggtgt attgggtacg gctatttgca aggtatttac catgaatgtt gcccgcaccg
     1321 gactttctga ggaacaagcc agagatggag gttatgattt tattacagta cttaatcctt
     1381 cacaggataa agcacatttt tatcccgatg caaaattaat tattattaag ttaattgttg
     1441 ataaaaaaag ccgtacattg cttggtgctc aaatagttgg acccggtgat gttgctaaac
     1501 gcattgaagt ggttattaca acaataacaa ataaggcaac catagatgac atagcaaatt
     1561 acgatttagc ttacgcacct ccattttctc ctgcaataga taatattatt atgactgcat
     1621 acatagcact aaataagtta gatggcatta ctaaatccat ttcaccagtt gaagttaaaa
     1681 agaaaatggg caataatgaa gattttattt tacttgatgt tcgttcacct caggaatatg
     1741 aagagattag aattgatgat gtccgggtaa aactaattcc attaggtaaa ttgcgagaat
     1801 ccctgcatca gcttgataaa tcaaaagaaa taataacttt ttgtaaaata tcattacgag
     1861 gatatgaggc acaaagaatt cttgaaggtg ctggttttag aaatgtaaaa tttttagatg
     1921 gtggaattgt atgctggccg tataaaaaat tggttaaata acatgattgc aggaggttat
     1981 gatgtttggt aataataaaa attcaaaatc caatgcggtg aaaccgggta tgaatgataa
     2041 acaggctatt gaagttgatg aagagttgaa attagaggaa aacctgaaca agattaagca
     2101 caccattctg gttttatctg gtaaaggtgg agttgggaaa agtactattg caacaaactg
     2161 tgccgtagcg cttgcatcgc gaaaattaaa aacgggtttg cttgatattg atattcatgg
     2221 cccaagtgtt ccaacacttc tgggcataga aaaattgggt ttgcaggcaa caaaccataa
     2281 tacaattatc cctatacaat ataatgacta tcttaaagtt atatcagcag gttttatgct
     2341 acccgacaat aaagaagctg ttatatggcg aggaccatta aaatacaatt taataaaaca
     2401 atttttatct gatgttgaat gggatgaact tgatttcctt attattgatt cccctccggg
     2461 aaccggtgat gaaccattat ctgttgcaca gcttgttaaa aatcctactg gtgctgtcat
     2521 agtcacaaca ccacaaaatg tttcaaccat cgatgtaaaa aaatcaatta ccttttgtca
     2581 taagcttaat ataccggtat taggtgttgt ggaaaatatg agcggatttg tatgccccca
     2641 ttgtggaaaa gaaacattca tattcaggaa aagtggcggc agtgaaattt cacaggagat
     2701 gggagtaccc tttttaggga gcatccctat tgatcctctt attgtggaaa acagtgatgc
     2761 cggcaggcca tttgcacagt cagcaaacag taatgctacc gcatctcaaa agcattttga
     2821 agaaataatc agcagtttac tgcaatcaat aactgtataa aaggagaaat gcatatgcta
     2881 atagctgtac cgttaacaca ggacaatatt ttttcattac attttggaca ttgcaataaa
     2941 tttgcaatgt atgatattga tgaaaatact aaatctattg tgaaaaaaga agaaattcct
     3001 gcaccgcctc accaaccagg tcttcttcca cgatggcttt ctgaaaaggg agctacatgt
     3061 attttagcag gtggcattgg acaaagagcc atgtcattat ttgaagaaca acatgtacat
     3121 gttgtcatag gtattgaagg tactgaccct gatgccattg taaacgatta tttagcaggg
     3181 aaactaaaag ttggtgctaa tgtttgtgat cattgatatg agttaaatat gagcactgta
     3241 tgtataacac tttatttaaa tggccattgc attgagagtg aagcaaaaaa ccagttacat
     3301 agactatatg aattaattct taattcatat aatgttacat ctgagcttga aaatcaatat
     3361 aagctgctgt atgattttat tacgatagct gattttaagg cactccgctc catggatgaa
     3421 cgtttaaacg gtttaatgcc ttcaatatgt gaattaacaa aggatgatga aggcaaaata
     3481 cagctacaaa ttcaaggatg caaccttttt ttcaacttct gaaatcgtta atcttatgac
     3541 taacgcgggc tattctaact ttcaatttaa acaaacactt tttgatgatg aaaatccctt
     3601 tagaattgaa gatggttata caggcggcag ttttgttgtt ataaaggttt taaatgagac
     3661 aaatatatta cattgtattc aaaatcaata ttaccataac atgtccttaa aaaagccgtt
     3721 gttataaaaa caataaattg ttttaaaaat atgtatatct aatataaata tttaaacatc
     3781 caatataaca acaaaacttt tggaatcaaa aaaaatatat ttgacgaaca taacagcgtt
     3841 atttgaatgt ccccaattta atgtttccat tacatgacaa tacatgacaa acggaggttg
     3901 ttatgagtga tatctactct gcactgcaaa aacgtcttga tatgtactcg cttggatttc
     3961 ccgaaactca atcaaagata gaaattacta tattaaaaaa gcttttttct gaagacgatg
     4021 caaaactttt catggaacta tcgcccatgc ttgaacctgc tgaaagcata gctgcacgta
     4081 tgggaaaacc tgtacaggaa atacaaaacc atttagagga catggtcaag cgggggcttc
     4141 tttttaaaaa aacaaaggat ggagttgcaa aatatggcgc tatccccttt atacacggca
     4201 tctttgaatt tcaggtaaag cgattaccca aagaccttgc cgaattactg gaacaatact
     4261 atgatgatgg acttgcaaaa gcaattgcaa caaatgccaa tatgtttttg catccgattc
     4321 cggtaaatga atccatagaa tcaaaaagca ctgttgcacc ctttgaagat gctgttgcaa
     4381 tcatacagca agctaatggt atagcggttg ctgattgtat atgtcgtaaa gaaaaaaaga
     4441 ccctcgggaa aggatgcgac aaacccatgg aaacatgcct tatgtttggt tcaatggctg
     4501 aatactatat tgaaaataat ctgggacgaa agataagtgc cgatgaggcg atagctatcg
     4561 taaaaaatgc acaaaaacag ggcatggtaa ctcagccagg cactgcacaa aaccctgcag
     4621 gtatgtgcag ttgctgtggc gattgctgtg cggtgttatt atctataaag cgatttccta
     4681 aacctgccga gatggtgttt tcaaactatt ttgcagaagt aaatgacaac tgtacatcat
     4741 gcggaagttg tgctgatata tgtcagatgg atgcaattga aatgaacgat tttgcatacg
     4801 ttaataagga tagatgtatt ggatgcggat tatgcgtttc agagtgccct gctgatgcca
     4861 tacaccttgt attaaaagac aacaaacgaa taccaccaaa aacaagtggt gagcagatga
     4921 tgcaaatggc aaagcaacgc ggcattttac ctgatggata agctaaagta ttattttttt
     4981 gaaattagta tcaccctgta gtatagttaa tagcatgaaa atgaaaattt caagctatcc
     5041 atttattctt tttattactc ttttgttatc atacagcagc tttgccagta ctgtccattt
     5101 tcatacctcc gctcatcata attgcccggt ttgcatatac cagcag
//
LOCUS       DAOSOX010000165         5140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16326_length_5140_cov_4.267650, whole genome shotgun sequence.
ACCESSION   DAOSOX010000165 DAOSOX010000000
VERSION     DAOSOX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16326_length_5140_cov_4.267650"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..1193
                     /locus_tag="PLX22_10130"
     CDS             9..1193
                     /locus_tag="PLX22_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002171108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HOT20295.1"
                     /translation="MQNMLATGKNVQFPRDNPVAATNQMLYKTALVEIDQYIKNIDES
                     KSKLDEVDSALQSVIRIFQRLRVLAVQGAHGIYTSFERKEAAATEINQMLEELVSIAN
                     IRGATGKPIFGGYQTGTENIPDPFVPIYQTLTAGNQGNAMIGVQYRGNIGKLQREVAT
                     GEYLDVNVPGNWAFWATNQILSANKDVSQYRTLATQAFKIDGVSIQLAAGDTIDIIVD
                     KINNAGLSVKASLGARNNLVLETTVPHALWLEDEGSGTFLRDAGMINPDFHNPPNNVD
                     PAVSVSGMSIFDMAIQLRDDLVRGDQELVGGRDLGLIDMALDNVLRHIAAVGARENRV
                     EELARRSEYAKSNVLDILSKSEGIDFPETIMNFRWLESVHNYALAVGARSIKPTLMDF
                     LR"
     gene            1217..1669
                     /gene="fliW"
                     /locus_tag="PLX22_10135"
     CDS             1217..1669
                     /gene="fliW"
                     /locus_tag="PLX22_10135"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOT20296.1"
                     /translation="MVTIVTKPFGQIEVDERQIIDFPEGIYGFEDIRKFAILDAKEKS
                     PFKWMQALDEPDLAFVIIRPADFMIKYELEVLPSDLEEIGATSPDEVIVFAIVTIPED
                     PSRMTANLQGPVIINPKTRCGKQAISLSENYSVRHYILEEIKKASVKG"
     gene            1674..1940
                     /gene="csrA"
                     /locus_tag="PLX22_10140"
     CDS             1674..1940
                     /gene="csrA"
                     /locus_tag="PLX22_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOT20297.1"
                     /translation="MLVLARKINESIMIGDDVEIVIIDIKGDQVKLGIKAPKTISVHR
                     KEIYDEIQKENIAAMKSQLKPETLKDISDILQSHKEKKIDNSAK"
     gene            2400..3068
                     /locus_tag="PLX22_10145"
     CDS             2400..3068
                     /locus_tag="PLX22_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOT20298.1"
                     /translation="MPDIIVKTNLIYLVAIFFVIFIIREIVAFRSILKLKYIFTPLIT
                     YIVIVIALYLSYISGFSQYDIFIIAGLVFALIADTFLMIEEINYFIHGLLFFLFMHIC
                     YIIALSIHYQFNYSDIIILAIIILISFSYYAVIHKAKGRLYVPVIIYMIVLSAVVLLA
                     IGTFIKTMSPKGALVMLAAIFFAVSDGILACNQFVKKIPHSTVITWSLYAPAQLLFAL
                     SCYY"
     gene            3173..4234
                     /locus_tag="PLX22_10150"
     CDS             3173..4234
                     /locus_tag="PLX22_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT20299.1"
                     /translation="MKKYIMLLLLLSVSCSQLIKPDSKREYENIYKHLTNAESLENPV
                     QKEKLLTRAYGIIQQSVLTKGEKADSRLLALLSYYYFLKGNYKDAEVNIGSAQPLSAD
                     DPFMAVLQARIMLATQGKSGVQAALGLLSPSEKYDNPMMYITTGDAYFLKGDYTTARD
                     NYKKALLLDKELQVVAANRLEVISRISTLTIDVSKVSDFILQPAMTRDKVAYLMYEIF
                     EIQKYISAVKPLDANFIDIEKSHYSTAIINLRKKGFFSYIDGGSFEPFMIVSRGEMAK
                     IVEDFLVLSKNNEGLRKKYAHESPSFFNDSKPDNPYYNALRLACDVGVMSVSLSQDAY
                     PDEAVTGLQAIMIIQKLVK"
     gene            4243..>5140
                     /locus_tag="PLX22_10155"
     CDS             4243..>5140
                     /locus_tag="PLX22_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20300.1"
                     /translation="MKKTLAIYSCVIAAFIAGVLAYAQFVTNTDKGQDERQKNKVTYK
                     NYIWIEGENAVSTNFAREPIYNFFCSNRFALQLAKDVDPPADGYYANYVFYVTHTKEY
                     DLWLGCTPPGSMYHDRPGYSSPIEWKVDNGPFQSASAENVYVKDFYGIGGFYWVKIAT
                     GTLNAGKHNLTIRVKQKRSSGWDYYFYIDAILFLPHYSIDIAELMNFPEVAPKNFTTP
                     GEGILFDDINEYEKKIRTNINDRGTLFTLVQIYGWLYDYNKAIVLLQRYLEKNPSDFQ
                     MRLLLAANYSWSDQLDAAINEYK"
BASE COUNT         1600 a          880 c         1107 g         1553 t
ORIGIN      
        1 tggatgccat gcaaaatatg cttgcaaccg ggaaaaatgt gcagttcccg cgtgacaatc
       61 ctgttgctgc taccaatcag atgttataca aaacagcact ggtggagata gatcaatata
      121 taaagaatat agatgaatca aaatcaaagc tggatgaggt cgactctgca ttgcaatcgg
      181 ttatccgtat ctttcaaagg ctgcgagttt tagcagtgca gggcgcccac gggatatata
      241 catcgtttga acgtaaagaa gcagcggcta ctgaaatcaa tcagatgctg gaagagcttg
      301 tcagcattgc caatattcgt ggcgccaccg gcaaaccaat ctttggtggc tatcaaacag
      361 gtactgaaaa tattccggac ccatttgtgc caatttacca aacgcttacc gcaggcaatc
      421 agggaaatgc catgattggt gtgcagtacc gtggaaatat aggcaagttg cagcgagagg
      481 ttgcaacagg tgaatatctt gatgtcaatg tccccggcaa ctgggcattc tgggcaacca
      541 atcaaatact ttcagcaaat aaggatgtgt cgcaatacag aacactggca acacaggctt
      601 ttaaaattga tggtgtgtcg atacagcttg cagcaggtga taccattgac ataatcgttg
      661 ataagatcaa taatgcggga ttaagtgtaa aagcatcgct gggggcgcgg aacaatctgg
      721 ttctggagac aacggttccc catgctctat ggctggagga tgaaggcagc ggaacatttt
      781 tacgcgatgc cggcatgata aatccggatt ttcacaatcc accaaacaat gttgatcctg
      841 cggtatcagt aagcgggatg tcaatctttg atatggctat tcaactcaga gatgatctgg
      901 tgcgaggcga tcaggagctt gttggagggc gtgatttagg gcttattgat atggcgcttg
      961 ataatgtgtt gcgtcatatt gcggcagttg gtgccagaga aaacagggta gaggagcttg
     1021 ccaggcgttc agaatatgca aaaagcaatg tacttgacat actgtcaaaa tctgagggta
     1081 tagatttccc cgaaactatc atgaatttca gatggcttga atcggtgcac aattacgccc
     1141 ttgcagttgg agcacggtca ataaaaccca cgcttatgga ctttttgcgt taaattagca
     1201 catacgaggt aaacagttgg ttacgattgt tacaaagcct tttggccaga tcgaagtaga
     1261 tgaacgccag attattgatt ttcctgaagg tatttacgga tttgaggata tacgaaaatt
     1321 tgccatactg gatgctaaag aaaaatcgcc ttttaaatgg atgcaagcgc tggatgaacc
     1381 cgatttagca tttgtaatta tccgtccggc ggattttatg ataaagtatg aacttgaagt
     1441 tttgccttct gatctggagg agattggtgc aacatctccg gatgaggtga ttgtttttgc
     1501 gatagttacc ataccagagg acccatccag gatgacagca aacctccagg gcccggttat
     1561 tattaatcca aaaacacgat gtggtaagca ggcaatatcg ctatctgaaa actattcggt
     1621 gcgccattac atactggaag aaataaagaa agcatcggtg aagggttaac attatgctgg
     1681 tactggcgcg aaaaataaat gaaagcataa tgattggtga tgatgttgag atagtcatca
     1741 ttgatattaa gggtgaccag gttaaattag gaataaaagc ccctaaaaca atctcggttc
     1801 acagaaaaga gatttatgat gagatacaaa aagaaaatat tgcagcaatg aaatcccagc
     1861 ttaaacctga aaccctgaag gatatttcgg atatattgca atcacacaaa gaaaaaaaaa
     1921 ttgacaattc cgctaaatag cacgataata gacataccgt tttactattg aatacatgct
     1981 tgatacattt ttcaacacgg tagcataact tttctggttg agttaccact atgtgcgata
     2041 ttgtcgtcaa aacatgctga atatgatatg aaccgtatcc ttaatccaca atgtacccgt
     2101 ctcagcaggg aggtggtgcc gatagctggc atggtgccat ccataaggga tgacgatgga
     2161 tcgttattgc cattctgagg caaagccgaa aaatcctgtc gttcaaagga tagatttttc
     2221 tgtagctaac gctccttcag aatgacaatg tgcggattgt tatcctgaac gctgattcag
     2281 gatctaacgt tagacatctg gattccgtat caagcccgga atgacagtga gaaataatgc
     2341 acttttttgc tgggaagtcc agggtgaggt taataatttt tttaaggaag gtttaatata
     2401 tgcctgatat aattgttaaa accaatctta tatatcttgt agctattttt tttgtcatat
     2461 ttatcatacg tgagatagtt gcatttagaa gtatcctgaa actaaaatat atttttacac
     2521 cgcttataac ctatattgtt attgttattg cactctatct gtcgtatata agcggttttt
     2581 cacagtatga tatttttatt atcgcagggc tggtatttgc attgattgcg gatacgttcc
     2641 ttatgattga agagataaat tattttattc atgggttgct attttttttg tttatgcaca
     2701 tatgttatat aattgcactg agcatccact atcagtttaa ttattccgat attattattc
     2761 ttgcaattat tatattaatt tctttttcat attatgctgt tatccataaa gcaaaaggca
     2821 ggctatatgt accggttatt atatatatga ttgttttgtc tgctgtcgta ttgcttgcaa
     2881 ttggcacatt tataaagacg atgagtccta aaggcgctct tgtaatgctg gctgctatct
     2941 tttttgctgt atccgatggg atactggcat gtaatcagtt tgtaaaaaaa atcccccata
     3001 gcactgttat aacatggtcc ctatatgccc ctgcacaact tctatttgct ttatcctgtt
     3061 attattaatg cttttggtat tttttcttgt aagttgcatt gcctgttgta tatactatga
     3121 acatttgatg ttaaggctgc agtggcatat atcaatataa atggtggaat atatgaaaaa
     3181 atacataatg ctgctactgt tgttgtcagt ttcatgttca cagttaataa aacctgattc
     3241 aaagcgagag tatgaaaata tatataaaca tttaacaaat gctgaatcgc tggaaaatcc
     3301 tgtacaaaaa gaaaaacttc ttaccagagc gtatggtatt atacaacaat cagtgttaac
     3361 aaaaggcgaa aaagctgaca gcagattgtt ggctctctta agctattact attttcttaa
     3421 aggcaactat aaggatgcag aagtaaacat tgggagtgcg cagccattat ctgctgatga
     3481 tccgtttatg gcagtgttgc aggcaaggat catgcttgca acacaaggta aatcaggtgt
     3541 gcaggctgct ttaggattac tatcgccctc tgaaaagtat gacaacccca tgatgtatat
     3601 tactacaggt gatgcttatt ttctaaaagg ggattatact acagcacgcg ataattataa
     3661 aaaagcttta ctgctggata aagaattgca ggttgttgca gcaaacagat tagaggtaat
     3721 ttcacgcata agtacattga caattgatgt ttcaaaggta tcggatttta tactccagcc
     3781 ggctatgacc cgtgacaagg ttgcctacct tatgtatgag atttttgaaa tacaaaaata
     3841 tatatcagca gtaaaaccac tggatgcaaa ttttatagat atagaaaaat cccactatag
     3901 cactgccata attaatttgc gcaagaaggg cttcttttca tatattgacg gaggtagctt
     3961 tgagccgttt atgatagttt caagaggtga aatggcaaaa attgtagaag attttttggt
     4021 attatcaaag aataatgagg gattgcgaaa aaaatatgca catgaatcgc catcgttttt
     4081 taatgatagt aaacctgata atccctatta taatgcattg agacttgctt gcgatgtggg
     4141 tgttatgagc gtttcgctaa gtcaggatgc ttatcctgat gaagcagtaa caggtttgca
     4201 ggctataatg ataatacaaa aattggtaaa gtaggggtgc gcatgaaaaa aacattagca
     4261 atatattcat gtgttattgc tgcgttcatt gcaggggttt tagcctatgc gcaatttgtt
     4321 acaaatactg acaaggggca ggatgaaagg caaaaaaata aagtaaccta taaaaattac
     4381 atctggattg aaggtgaaaa tgctgtatct actaattttg cccgtgaacc tatctataac
     4441 tttttttgta gtaacaggtt tgcattgcag cttgcaaaag atgttgatcc cccggcggat
     4501 ggctattacg ccaattatgt tttttatgta acccatacta aagaatatga tctttggttg
     4561 ggatgcacac ctcctggctc aatgtatcac gatagacctg gctattcatc accgattgaa
     4621 tggaaggttg acaatggacc ttttcagagt gcctcggctg aaaatgttta tgttaaggat
     4681 ttttatggta ttggtggatt ttactgggtt aaaattgcca caggaacgct caatgcgggg
     4741 aaacataatc ttaccattcg tgtaaagcaa aagcgaagca gcgggtggga ttactatttt
     4801 tatattgatg cgatactatt tttaccccat tacagcattg atatagccga attgatgaat
     4861 ttccccgaag ttgctcctaa aaattttact actccaggtg aaggaatact gtttgatgat
     4921 attaatgaat atgagaaaaa aatcaggaca aatatcaatg ataggggaac cctttttacg
     4981 ctggtacaga tttatggatg gctttatgat tacaacaagg ctatagtgct tttacaacga
     5041 tatttggaaa aaaatcctag cgattttcag atgcgactgc ttttagcggc aaactattca
     5101 tggtctgatc agttagatgc agcaataaac gaatataaaa
//
LOCUS       DAOSOX010000166         5095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16537_length_5095_cov_9.961905, whole genome shotgun sequence.
ACCESSION   DAOSOX010000166 DAOSOX010000000
VERSION     DAOSOX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16537_length_5095_cov_9.961905"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLX22_10160"
     CDS             complement(<1..469)
                     /locus_tag="PLX22_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT20301.1"
                     /translation="MEALPWYREYKILGIPKTFKPYPDKPVYDILEIAAKQYRKNGLI
                     QENFFLSYPELLIYVNKLTSAFHAFGLKKNDIIATILPTSTEFIICDYAISRAGLIHL
                     PCSALEPAEALEHKFKEAKPKAIICTVIGDHITVVKNLMKKFSLQFIIVTTIRK"
     gene            complement(549..1106)
                     /locus_tag="PLX22_10165"
     CDS             complement(549..1106)
                     /locus_tag="PLX22_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOT20302.1"
                     /translation="MAITSNTTVEQFLTEIMPKEALGMLQNSNAPKELNGTEITMVVE
                     VDGKSYGFKVNDGKDITPCGDIPNAMLRLKISEADLKKMIDTNNLDMLLGMQKDLTRA
                     RYNALSSLKGSFIAKLKNDDGSEFTIEAILNGATSPQSIFRMSTKDSAALMKKETNPV
                     NLFMSGAMKIEGDMSFAMATQPLFT"
     gene            1323..1937
                     /locus_tag="PLX22_10170"
     CDS             1323..1937
                     /locus_tag="PLX22_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT20303.1"
                     /translation="MSRADETRDKILLYAKKLFSEKGYYSSQISDIVESAKIGRGTVY
                     QYFSNKEHLFISLLERFLIEWEEYIKELSVSEDLSIISHYTYLKRRLYKAFKFFFSDR
                     DRANIILRAGIGLPEHFEQKIQHFENKLINMIIADLAIAVEFNHIDMNEDLSFAANLV
                     AGGVFRLAHYYLLIESNDEVLLQNLDDISSKAAHFIGNGIFSKK"
     gene            2001..3098
                     /locus_tag="PLX22_10175"
     CDS             2001..3098
                     /locus_tag="PLX22_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20304.1"
                     /translation="MVEEISFALSEEQQMMQDTVRQLVQATIADGIHQMDENRQLDKQ
                     AVQKFWELGIIQSMIPEEFGGYGMDSSPVLHTLVLEELAYGDMTFAIYAMLPAVFVLP
                     IVHLGAKEQKELYLPEFCNATFVPATLAINELSVAFDVWNCATTAETKGDKYIINGSK
                     CFVPLADESNFMIVVAQCEGNPELFIVTRDNSGIKVGEREKNCGWWALPTFEVTFENC
                     IIPASHRLGDSTAITWLLQRTRTALSAIGTGISRASYEYAKKYAKERQQFGEPIASRQ
                     AIAFMIAEMAYEVDAMRLLSWKAASTIENNLDATRLSYLAKMYAGEMTMKITDYGVQI
                     LGGHGYIRDHPVERYYRNGRSIALCEGMAII"
     gene            3117..4313
                     /locus_tag="PLX22_10180"
     CDS             3117..4313
                     /locus_tag="PLX22_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20305.1"
                     /translation="MIQLVTPEYLVTLQNNMRKLAEGVLRPISRKYDEAEHEYPKELD
                     MFRNIKVMARPKKKKDGEKSSSGEEKKPKRGANLGMVATIEEMCWGDAALLLSFPNAG
                     LGNAAIIAVANDEQFERFGNKWAAMAITEPGAGSDSGAITTTATRDGDFWVLNGEKIF
                     VTCADRSEVVVVWATVDKKAGKSGIKSFVVEKGTPGFTLEHLEHKLGIRASDTGTFVL
                     SDCRIPYNNILGDPEVKMSTEGFKGVMKTFDNTRPMVAAMATGIARAAVEFTKEQFEQ
                     KGYTFDYKKNTANISAVERELYMMEANVEAMRLLTWRAAWMADNGQFNNLEASMAKAK
                     AGRYATLVTQRCCELLGPVGYSTHYLAEKWMRDSKIMDIFEGTGQIQHLIVARNVLEM
                     SSKELK"
     gene            4356..>5095
                     /locus_tag="PLX22_10185"
     CDS             4356..>5095
                     /locus_tag="PLX22_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20306.1"
                     /translation="MNTIDDVKNIAVIGSGAMGHGIAQVCAQNCFPVTMIDIKQEFLD
                     TALKKVKESLDFLESKGKLNGTVDEVMKKINVTTDMKKGVSDAQVIIEAVPENMKLKQ
                     EVFSQASAACAADAILATNTSTMSITDIATAVNNPERFAGMHFFNPVTRMKLVELIYG
                     AKTSEKTIDILYELAKKIDKIPVRVLKDRPGFIVNRINAPNQALLSAILDEGKIHPAQ
                     IDGKMKKMGVKMAPFETADFVGLDVFCHT"
BASE COUNT         1655 a          781 c         1075 g         1584 t
ORIGIN      
        1 ttttgcgtat tgttgttact ataatgaatt gtaaggaaaa ctttttcatt aaatttttta
       61 ctactgtaat atgatcgcca ataacagtgc agataattgc tttaggtttt gcctctttaa
      121 atttatgctc aagcgcttca gctggttcca gggcactaca tggtaaatgg attaaccctg
      181 cacgcgatat tgcataatca catataataa attctgttga agtgggaagt attgttgcta
      241 tgatatcatt tttttttagt ccaaacgcat ggaatgcaga tgtcagctta ttaacatata
      301 ttaataattc gggatatgat aaaaagaaat tttcctgtat aagtccattt tttctatatt
      361 gttttgctgc tatctcaagt atatcataaa ccggtttgtc gggatatggt ttaaaggttt
      421 ttggtatacc taaaatttta tattctctat accacggtag tgcttccatt gcttacctcc
      481 ataagacata aaaataaaaa aatatctcaa ccacattgat gtatgtggtt gagataaaca
      541 atcatttatc aagtgaatag gggttgggtt gccatagcaa agctcatgtc tccttcaatc
      601 ttcattgcgc ctgacatgaa aagatttact ggattggttt cctttttcat caacgctgca
      661 gaatcttttg tactcatacg aaaaatagac tggggggatg tggcaccatt gagaattgct
      721 tcaatagtaa attctgaacc gtcatcattt tttagtttag ctatgaagct gccctttaat
      781 gatgatagtg cattgtaacg ggcacgtgtc agatctttct gcatgcccag caacatatca
      841 agattattgg tgtcgatcat ctttttaaga tcagcttcag aaatttttaa tctcagcata
      901 gcattaggga tatccccaca aggtgtgata tctttaccat cgttaacctt gaatccatac
      961 gactttccat ctacttcaac taccatagta atttctgtgc catttaattc ttttggtgca
     1021 ttggaatttt gaagcattcc caaagcttct ttaggcataa tttcggttaa aaattgttca
     1081 accgtggtat tggatgtaat agccataatc atcctcctac tataaaaaat ttaaaacgta
     1141 caggaaataa aaatttttct tgaaatactg acatgtcagt actataatgt tttgtcaaga
     1201 ttttttctaa aaattttcta aaaattttta gtgctggata gttattctat aataattttg
     1261 ctttgcttac tatccataat aagtgtaatg gtacacaatg taataagttt tggaggtaac
     1321 caatgtcaag ggctgatgaa acgcgagata aaattttatt gtatgctaaa aaattatttt
     1381 ctgaaaaagg atattattca tcacagattt ctgatattgt tgaatctgct aagattggca
     1441 gaggaactgt ctaccagtat ttttcaaata aggagcacct ctttatatca ttacttgaaa
     1501 gatttttgat tgaatgggaa gaatatatta aagaactatc agtcagtgag gatctaagta
     1561 ttatttcaca ttacacctat ttaaaaaggc gtttgtataa ggcatttaaa ttttttttta
     1621 gcgacagaga tagagcaaat attattttaa gggcggggat tggtttgccc gagcattttg
     1681 aacaaaaaat acaacatttt gagaataagc tgataaacat gattattgct gatctggcaa
     1741 tagctgttga atttaaccat atagacatga acgaagattt aagttttgca gctaatcttg
     1801 tagctggtgg tgtattccga ttggctcatt actatctttt gattgaatcc aatgatgagg
     1861 tactgttaca gaatttagat gacattagca gcaaagcagc acattttatt ggaaatggta
     1921 tttttagtaa aaaatagttg actaattttt atggttgata aagtactgac atgtcagtac
     1981 tattattatg ggaggtaagt atggtagaag agattagttt tgctctatct gaggaacaac
     2041 agatgatgca ggatactgta cggcagttgg tgcaggctac catcgcagac ggtatccatc
     2101 agatggatga aaataggcag ctggacaaac aggctgttca aaaattctgg gaattgggca
     2161 ttattcaatc aatgattcca gaagaatttg gcggttatgg gatggatagt tcacctgtat
     2221 tgcatacgct tgtattggaa gaattggctt atggggatat gacatttgca atatatgcaa
     2281 tgcttcctgc tgtttttgtt ttgccaatag tacatttagg agcaaaagaa caaaaagaac
     2341 tatatcttcc agaattttgt aatgcaacat ttgttcctgc tacattggct atcaatgaat
     2401 taagtgtagc ctttgatgta tggaactgtg caactactgc tgaaacgaaa ggggacaaat
     2461 acatcatcaa tggcagcaaa tgctttgtgc cgctggctga cgaatcaaac tttatgattg
     2521 tcgtggctca atgtgagggt aacccggagc tctttatagt taccagagat aactctggaa
     2581 taaaagttgg agaacgggaa aaaaattgtg gatggtgggc attgccaact tttgaagtta
     2641 cttttgaaaa ttgtatcatt cctgcatcac atagattagg tgatagcacc gctattacat
     2701 ggttgctgca acgaacaaga acagccctta gtgcaattgg tacaggtatt tcacgagcat
     2761 cgtatgaata tgcaaagaaa tatgcaaaag agcgtcagca atttggtgaa ccaatagcca
     2821 gccgacaggc aattgcattt atgatagctg aaatggcgta tgaggtagat gctatgcgac
     2881 tgttatcatg gaaggctgct tcaaccattg aaaacaattt agatgcgaca aggttatcat
     2941 atttagcaaa gatgtatgca ggtgaaatga ccatgaagat tactgactat ggtgtccaga
     3001 tacttggagg ccatggttat attcgcgacc atcctgttga gcggtattat cgcaatggta
     3061 gaagcatagc actatgtgaa ggtatggcaa ttatataaac cagtggaggt atatgtatga
     3121 ttcaattagt tacacctgaa tatttagtaa cattacaaaa caatatgcgc aaattggctg
     3181 aaggagtatt gcgccctata tcacgaaagt atgatgaagc tgaacatgaa tatccaaaag
     3241 aacttgatat gttcaggaat attaaagtta tggctcgtcc caagaaaaaa aaagacggtg
     3301 aaaaatcatc atctggtgaa gaaaaaaaac caaaacgagg tgcaaattta ggaatggtag
     3361 caactattga agagatgtgc tggggtgatg cagcgctgct attatcgttc cccaatgctg
     3421 gtttgggcaa tgcagctatc attgcagttg ctaatgatga acaatttgaa cgattcggaa
     3481 ataaatgggc cgcgatggca attaccgagc caggtgctgg ttctgattcg ggtgccatta
     3541 ccactacagc aactcgggat ggtgacttct gggtattgaa cggtgaaaag atatttgtaa
     3601 cctgtgctga tagaagtgag gtggtagttg tttgggcgac agttgataaa aaggctggca
     3661 aatcaggtat taaatcattt gtagtagaga aaggaactcc aggatttaca ttagaacatt
     3721 tagagcataa attgggtatc agagcctcag ataccggaac ctttgtttta agcgattgta
     3781 gaatacccta taacaacata ttaggcgatc cagaagtaaa gatgtccact gaaggcttta
     3841 aaggagtgat gaaaaccttt gataataccc gaccaatggt tgctgctatg gctaccggta
     3901 tagcacgagc agcagtagaa tttactaaag aacaatttga acaaaaggga tacacatttg
     3961 attataagaa aaatactgca aatatttccg cagttgaacg tgaattgtat atgatggagg
     4021 caaatgtaga agcaatgcgt cttttaacat ggcgtgctgc atggatggct gataatggtc
     4081 aatttaataa tcttgaagct tcaatggcaa aagcaaaagc cggacggtat gcaacactgg
     4141 ttacgcagcg ctgctgtgag cttttagggc ctgttggcta ttcaactcac tatttagctg
     4201 aaaagtggat gagagacagc aaaattatgg atatatttga gggtacggga caaattcagc
     4261 atcttattgt tgcccgcaat gtactggaaa tgtcaagtaa agagttaaag taatatagat
     4321 ttataatcat tatcattatt tcataggagg tgtttatgaa tactatcgat gatgtaaaga
     4381 acattgcagt tataggctca ggtgctatgg gacatggtat agcacaggtt tgtgcccaaa
     4441 attgctttcc ggttaccatg attgatatta aacaagagtt tttagatact gcattaaaaa
     4501 aagttaagga aagtttagat tttttagaat caaaaggaaa actgaacggc acagttgatg
     4561 aggtgatgaa aaaaataaat gttacaactg atatgaaaaa aggggtgtct gatgcgcagg
     4621 ttatcattga agctgtacct gaaaatatga agcttaaaca agaggtattt tcacaagctt
     4681 ctgctgcatg tgctgctgat gcaatattag ccaccaatac ttcaacgatg agtattacag
     4741 atattgcaac tgcagtaaat aatcctgagc gatttgccgg gatgcatttt tttaatccag
     4801 taacaagaat gaaattagtt gaattgatct atggtgcaaa aacatcagaa aaaactatcg
     4861 atatattgta tgaacttgcc aaaaagattg ataaaattcc tgtgagggtt ttaaaagata
     4921 ggccgggatt tattgttaat agaatcaatg caccaaatca ggcactactg agcgctatcc
     4981 ttgatgaagg gaaaattcat cctgcacaga ttgatggtaa aatgaagaag atgggtgtta
     5041 aaatggcgcc ttttgaaaca gcagattttg ttggacttga tgtattttgt catac
//
LOCUS       DAOSOX010000167         5040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16769_length_5040_cov_9.310331, whole genome shotgun sequence.
ACCESSION   DAOSOX010000167 DAOSOX010000000
VERSION     DAOSOX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16769_length_5040_cov_9.310331"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2615
                     /locus_tag="PLX22_10190"
     CDS             <1..2615
                     /locus_tag="PLX22_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT20307.1"
                     /translation="NIYMRSLATRGSKSSVSHYIQDAIDILKLAGFDVIMLETSGIGQ
                     AQTDVLDYCNCSIYVMTPEYGAATQLEKIDMLDYADIVVINKFDKSGALDALHDVKKQ
                     MQRNKEAFDMPLEQMPVFGTIASQFNDDGVNKFFNYLIEKLNEKLNEKGGKKFAAYVA
                     EKQKKDIVYIVPPERTRYLSEIAETIKKYNNNTLEESKKALYLYSIKKCIEYYKQSKS
                     EHSVIDELNKKYHEVYGSLSADSKTILKTWEDKKKNYQGEYYTYHVRNKEIKVKAYTE
                     SLAHTKIPKVSVPKFENWGDIITWNMQENFPGEFPYTAGVYPFKREDENPTRMFAGEG
                     IAEKTNKRFHYLSYNMPAVRLSTAFDSVTLYGYNPDRRPDIYGKIGNSGVSISTIDDV
                     KRLYSGFKLTDPSTSVSMTINGPAPIMAAYFFNAAIDQECEVYIQQNGLTEEVKKKIE
                     ALYNERNAKKPEYRAEQLPDGHNGLGLLLLGISGDMVLPQEIYNNIKAQTLRKVRGTV
                     QADILKEEQAQNTCIFSIDFALKLMGDMQEYFIKHTINNFYSVSISGYHIAEAGANPV
                     TQLAFTLANGFTYVEYYLARGLAINDFAPNLSFFFSNGLDPEYSVIGRVARRIWAKAM
                     KYKYGGNERSQMLKYHIQTSGRSLHSQEIEFNDIRTTLQALYAIYDNCNSLHTNSYDE
                     AITTPTEESVRRALAIQMIINQEYGLAKNQNPLQGAFIIEELTDLVEEAVIAEFNNIS
                     IRGGVLGAMETMYQRNKIQEESLYYENKKMTGELPIIGVNTFLSKDGSPIQIPNEVVR
                     ATPEEKDVQLESLAQFHRVWKPEADIALQKLKQIVKQHENVFETLMDVAQYCSLGQLT
                     DALYSVGGMYRRSM"
     gene            2618..3916
                     /locus_tag="PLX22_10195"
     CDS             2618..3916
                     /locus_tag="PLX22_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT20308.1"
                     /translation="MNLFNKDTYVPLAERMRPQTIEEFVGQRHLLSKGKLLYSVFDQK
                     KALSIILWGDPGTGKTTLARLIAHTCRMDSYFISAISAGVADVRKVIEKGRDNRSQGI
                     QTLLFIDEIHRFNKAQQDAVLGAVESGDIVLIGATTENPSFSIISPLLSRTRVLRLYP
                     LQEDDIRVIVTRALTHDQELSHKNISIPDEALDSISQLAMGDARRALNILEAAVFLNG
                     EGELNKEIILEAYREQSIAYDKSGDKHYDTISAFIKSLRGSDPNAAIYYLARMLVAGE
                     EPEFIARRMVIFASEDIGNAYPLALTVATSGMIAVKNIGLPECEIILAHMATFLASCP
                     KSNASYMALLNAKEVVQQGIHEIPLYLRNAPTKLMQQFGYAQGYKYPHEYPGHFVDEQ
                     YLPDAIKELIFYTPSLEGNEKNIRERLKKLWPKKYKENNH"
     gene            3913..4476
                     /gene="pyrE"
                     /locus_tag="PLX22_10200"
     CDS             3913..4476
                     /gene="pyrE"
                     /locus_tag="PLX22_10200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT20309.1"
                     /translation="MTNKERLFEILYQKSFMYRDNPPFTLVSGKQSPYYFDCKATTLY
                     PEGCILTGKCMYALIQDIPADAIGGLTLGADPISLSTAMEAYLQGRILYPLIVRKEPK
                     KHGTQKWIEGDITRVKNVIAIDDVITTGGSTITAIERLTESGLQVVAAAVIIDREEGG
                     REAIEEKGITVYSLFTRSDFDKKRLSH"
     gene            complement(4537..>5040)
                     /locus_tag="PLX22_10205"
     CDS             complement(4537..>5040)
                     /locus_tag="PLX22_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT20310.1"
                     /translation="LVVLIVFTASTVFAAGIGVKGGYSKMYDDYSDWFDDTWNIGVYF
                     DLGKFLFDTLQFRPGLDYLSLEPDEGDGDVDVYGIHIDWYWFFMGKKQISPYLGFGPA
                     LNYYDFDSNSTIDEDSDAGIEGFAGVEFNLTGPWTLLVELRYLWHDIADRDTTIVKFN
                     VGLMYYF"
BASE COUNT         1676 a          920 c         1062 g         1382 t
ORIGIN      
        1 ccaatatcta tatgaggtca ctggctactc gaggctcaaa gtcttctgtg tctcactata
       61 ttcaggatgc gatagatatt ttaaagttag ccggttttga tgtcattatg cttgaaacct
      121 ctggaattgg gcaggcgcag acagatgttc tggattattg taattgttca atttatgtca
      181 tgacccctga atatggagct gctacacaat tggaaaaaat tgatatgctg gactatgccg
      241 acatagtagt tataaataaa tttgataaat caggagcact tgatgcgttg catgatgtaa
      301 aaaaacagat gcagcgcaat aaagaagctt ttgatatgcc gcttgagcag atgccggtat
      361 ttggaactat tgccagtcag tttaacgatg atggtgtcaa taaatttttt aattatctca
      421 ttgaaaaatt aaatgaaaaa ttgaatgaaa aaggtggaaa aaaatttgct gcgtatgttg
      481 ccgaaaaaca aaagaaggat atagtatata ttgtaccgcc tgaaagaacc agatatcttt
      541 ctgaaatagc agaaacaata aaaaaatata ataataacac tctggaagaa tcaaaaaaag
      601 ccctttatct ttatagcata aaaaaatgta ttgaatatta caaacaaagt aagagtgaac
      661 attcagtaat tgatgaactt aataaaaagt atcatgaggt atatggatca cttagcgctg
      721 acagcaaaac aattttaaaa acatgggagg ataagaaaaa aaattatcag ggtgaatatt
      781 atacatatca tgtacgaaat aaagaaataa aagtaaaagc atataccgaa tcattggcac
      841 atacaaaaat ccccaaagtt agtgtcccaa aatttgagaa ctggggcgat atcattacat
      901 ggaatatgca ggaaaatttt cctggcgaat tcccctatac cgccggcgta tatcctttta
      961 aacgtgaaga tgaaaatcct acccgtatgt ttgcaggtga aggcatagca gaaaaaacaa
     1021 ataaacgttt tcattatctt tcatataata tgcctgctgt gcgtctatca actgcttttg
     1081 attctgttac attgtatggt tataatccag ataggcgacc tgacatttat ggaaagattg
     1141 gcaattcagg tgtttctata tcaactattg atgatgtaaa aaggttatat tcaggattta
     1201 agcttactga tcccagcaca tcagtttcaa tgaccatcaa tggtcctgct ccaataatgg
     1261 cagcttactt ttttaatgca gctattgatc aagaatgtga agtatatata cagcaaaatg
     1321 gtttaactga agaggtaaaa aagaagattg aagcattgta taatgaaaga aatgctaaaa
     1381 agcctgaata tagagcagag cagctgccgg acgggcataa tgggctgggt ctgctactgc
     1441 ttggtattag tggtgatatg gttttacctc aggagatata caacaatatt aaggcacaaa
     1501 cgttgcgaaa ggttaggggc actgtgcagg cagatatatt gaaagaggaa caggctcaga
     1561 atacatgtat tttttcaatt gattttgcat tgaaactgat gggggatatg caggaatatt
     1621 ttataaagca taccattaat aatttttact cggtgtctat ttcaggatat cacatcgctg
     1681 aagctggtgc aaatcctgtt acgcaactgg cttttacgtt agctaatgga tttacctatg
     1741 ttgaatacta tctggcacga gggcttgcca ttaatgattt tgctcccaat ttgtcatttt
     1801 tcttttcaaa tggacttgat cctgaatatt ctgttatagg acgggttgcg cgcaggatat
     1861 gggcaaaggc aatgaaatat aagtatggtg gtaatgaacg ttcacagatg cttaaatatc
     1921 atatacaaac ttcggggaga tcgcttcatt cacaggagat tgaatttaac gatatacgta
     1981 ccacccttca ggctttgtat gccatttatg ataattgtaa ttctttgcat accaattcat
     2041 atgatgaagc aataaccacg cctacggaag aatcggtgcg ccgggcatta gcaatacaga
     2101 tgattattaa ccaggaatat ggactggcaa aaaatcagaa cccactgcag ggagcattca
     2161 tcatagaaga gttgactgat ctggtggagg aggctgttat tgctgagttt aacaatatca
     2221 gtatacgcgg tggcgtttta ggagccatgg aaaccatgta tcagcgcaat aaaattcagg
     2281 aagaatcact ttactatgaa aataaaaaaa tgaccggtga attgcccatt ataggagtaa
     2341 acacctttct ttcaaaggat ggctcaccca tacagattcc caatgaggtt gtacgagcaa
     2401 cgccggaaga aaaagatgta cagcttgaat cattagcgca atttcacagg gtatggaagc
     2461 cggaagccga tatagcctta caaaaattaa aacaaatagt aaaacaacat gaaaatgtgt
     2521 ttgaaacatt aatggatgtt gctcaatatt gctctttagg acagctaact gatgcactct
     2581 acagcgttgg tggtatgtat agaagaagca tgtaataatg aatctattta acaaagacac
     2641 ctatgttccc ttagccgaac ggatgcgccc gcaaacaatt gaggaatttg ttggtcagag
     2701 gcacctgctt tcaaaaggca aactgttgta ttctgtcttt gatcagaaga aagctctgtc
     2761 gattatcctg tggggtgacc cgggaacggg caaaactacc cttgcaaggc ttattgcgca
     2821 tacatgccgg atggattcgt attttataag cgctatttcg gcaggcgttg ctgatgtgcg
     2881 caaggttata gaaaaaggaa gggataaccg ttcacagggc attcaaacat tgctttttat
     2941 tgatgagatt caccgcttca acaaagctca acaggatgca gtgcttggtg ctgttgaaag
     3001 tggtgatatc gttttaatag gagcaaccac tgaaaatcct tcgttcagca ttatttcgcc
     3061 gttattgtcg cgaacccgtg ttttacggct gtatcctttg caggaggacg atataagggt
     3121 gatagttacc cgggcattga ctcatgatca ggaactatcg cacaaaaata taagtatacc
     3181 tgatgaagca ctggacagta tatcacaact ggcaatgggt gatgcgcggc gagcattgaa
     3241 catacttgaa gcagcggtat ttttaaacgg tgagggggaa ctcaataaag agattatact
     3301 ggaggcatac cgggagcaga gcattgctta tgataaaagc ggtgataaac attatgatac
     3361 gatatcagca tttataaaat cattgcgagg ctctgacccc aatgcagcta tctattattt
     3421 agcacggatg cttgttgccg gtgaagaacc cgaatttatt gcacgccgaa tggtaatatt
     3481 tgcttcggag gatattggca atgcgtatcc acttgcatta acggtggcaa cctcagggat
     3541 gattgcggta aagaatatag gactgcccga gtgtgagata atactggcac atatggcaac
     3601 ttttctggca tcgtgtccca aaagcaatgc atcgtatatg gcactgctca acgcaaagga
     3661 ggttgtgcag cagggtattc acgagatacc gctgtattta cgaaatgctc ccaccaaact
     3721 tatgcagcag tttggctatg ctcaagggta taagtatccc catgagtatc ccggccattt
     3781 tgttgacgaa caatatttgc ccgatgcaat aaaagaattg attttttaca cgccatcgtt
     3841 agaaggtaac gaaaaaaaca tcagggaaag attaaaaaag ctgtggccaa aaaaatacaa
     3901 ggagaacaac cattgaccaa taaagaacgg ctatttgaaa tactctatca aaaatcattt
     3961 atgtacaggg acaacccgcc atttaccctt gtttcgggga aacagagccc atactatttt
     4021 gattgcaagg ctaccacgtt atatcctgaa ggatgcatcc tgacgggtaa atgtatgtat
     4081 gcattaatac aggatatacc tgctgatgcc attggcggat taacattggg agccgaccct
     4141 atttcgttgt caactgcaat ggaagcatac ttgcagggca ggatacttta cccgttgata
     4201 gtgcgcaaag aaccaaaaaa gcacggtaca cagaaatgga ttgaagggga tatcaccagg
     4261 gtaaaaaatg tcatagctat tgatgatgtc attactaccg gcggatcaac tatcaccgca
     4321 atagaaaggc ttactgaatc gggcttacag gtggttgctg cagcggtcat tatagacagg
     4381 gaggaaggcg gcagagaagc tattgaggaa aaaggtatta ccgtatattc tctctttacc
     4441 cgcagtgatt ttgataaaaa aaggttatca cactaagcaa tatacactat ggccatggcg
     4501 attgtttcat cgccatagcc atagaattat catcatctaa aaataataca tcaacccaac
     4561 gttaaatttt actatcgttg tatccctgtc ggctatatca tgccatagat agcgcagttc
     4621 aaccagtaat gtccatggac cggtcaaatt aaattcaaca ccggcaaagc cttctatacc
     4681 ggcatcagaa tcctcatcta tggtagaatt actgtcaaaa tcatagtaat ttaaagcagg
     4741 tccaaatcct aaatatggcg aaatttgctt tttgcccatg aagaaccagt accaatcaat
     4801 atgaatgcca taaacatcaa catcgccatc accttcatct ggttcaagtg atagataatc
     4861 aagccctggc ctgaactgca atgtgtcgaa taaaaattta cctaaatcaa aatatacacc
     4921 aatattccat gtgtcatcaa accagtcaga gtaatcatca tacatctttg aatagccacc
     4981 tttgacacca atacccgcag caaaaaccgt tgaagcagta aatactatga gtaccactaa
//
LOCUS       DAOSOX010000168         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16825_length_5026_cov_10.172199, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000168 DAOSOX010000000
VERSION     DAOSOX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16825_length_5026_cov_10.172199"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PLX22_10210"
     CDS             complement(<1..1256)
                     /locus_tag="PLX22_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20311.1"
                     /translation="MALPSSTQSSLCRFALQDEEKLSDEHVKQEIQKAFDEHRAFPFV
                     VDSSIIDAIRKGVDTAILRDSFLAIRDAMVLSMSNSSQSTDKLLADIQLQTRIVNAIT
                     KIHVSIAQGNFTSIVSRRFVLIKERPNWPTLYYVSPQSAVLSHVGQGPQWTEIPTIYL
                     GLNIFNVLTLDSTNTYDRAFYWLLKVEERAIETGYSHIEVYPPHVSVALNQLLDEVIR
                     IATLSEVEFKEIPERKKIRPFTARNRESFLKMLNARNPDDSFDFEKNLKAIRSLERLA
                     RRYKKGYDVASLREIVRLLVAASGHDIHEIRNSANILLERIFAPKEFDAPLATSFINC
                     ANNSTHLFEFIIPEEGDYFVRIFSNAGSNQFPLEQDIEFFDIDCIYKEKKGVYTARYQ
                     FTRIGSYDYCVYKKRKKRNQWLRYEG"
     gene            complement(1279..2025)
                     /locus_tag="PLX22_10215"
     CDS             complement(1279..2025)
                     /locus_tag="PLX22_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20312.1"
                     /translation="MNTTQHYNSALPFLFLGIVLVIVTVSCTSVEFVKTGNEYTPLPP
                     NSDVQVLLTVDKNQFDEIGIADIGGVSLPARIEKAKEIARSKGGDVIVPAIMCNEESK
                     KRMRSGYIIQSFYILKTRSTAIIQKPVEETRSFTQMVEKELLPEKRSATFQQLLREYP
                     QLHGEVFTNTMQPSGFYKIPPSLLVYGLSQHKLCLMKFDEENSALVFIPMDKINTIKA
                     SIEGKKSLSIDYTPLGVYKEKYPVLKMVSQ"
     assembly_gap    2156..2255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2634..3806
                     /locus_tag="PLX22_10220"
     CDS             2634..3806
                     /locus_tag="PLX22_10220"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT20313.1"
                     /translation="MKEVVIVSAVRTPLGNFNGALSSMPATQLGAIVIEEAVKRAGIQ
                     KEDVDEVIMGQVLPCGYGQNPARQAAIIASMPWEVGCLTINKVCGSGLKAVMLAAQAI
                     QAGDAEVIVAGGMENMSMAPYYLEKARFGYRMGDSPLKDHMVHDGLWDIVNDFHMGIS
                     NELCSEKYGISRQEQDRYAEASYTKAMHAIASGAFKKEIVPVTIAQKQRTVVFDTDEC
                     PKETPYEVLAAMKPSFKAGGVTTAGNSSVISDGAAALVVMSKEKADKLGCTIMATVGA
                     QASAGIDMKYVLVAPIWAIPKCCKKEGVNVEDIELYEVNEAFSGSSVAVLKELKLDAG
                     KVNVNGGSVALGHPIGASGARVLVTLVHEMIARDKKSGLASLCLGGGEAVALIVKR"
     gene            3824..4684
                     /locus_tag="PLX22_10225"
     CDS             3824..4684
                     /locus_tag="PLX22_10225"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOT20314.1"
                     /translation="MQVNDIKVFGVVGAGQMGHGIACVAALSGLTVILNDISMEYVNK
                     GIQGIDAVLTRNVDKGTLTHEEKDAVLHRIKPCTALNDLADADFIVEAASENPQIKWK
                     LFSDLDALCPSHVVLATNTSSIPIGRIASVTRRADKVIGMHFMNPVPVMKLVEVIRAL
                     ATSDETFDVTWNLSIKFGKTPALANDYPGFIANRILMPMINEAIYSLYEGVGTRESID
                     TVMKLGMNHPMGPLALADLIGLDTCLAIMETLYEGFKDTKYRPCPLLRRYVEAGWLGC
                     KTKKGFYDYT"
     gene            4702..>5026
                     /locus_tag="PLX22_10230"
     CDS             4702..>5026
                     /locus_tag="PLX22_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20315.1"
                     /translation="MHFELSEEQIIIQETASRFAKQELEPAAAVLDATKNREILKSNL
                     KKLAKLGFNGIAVDPRYGGTGAGSVAFSLVLTQLGKACAATAVTTSVTNMVAEVIQSV
                     GTEEQK"
BASE COUNT         1460 a          904 c         1161 g         1401 t
ORIGIN      
        1 cacccttcat agcgcagcca ctggtttctt tttttccgct ttttataaac acagtagtca
       61 taggatccta tccgggtaaa ctgatagcgt gcagtatata cgcctttttt ttctttataa
      121 atgcaatcaa tatcaaaaaa ttcaatatcc tgttctaacg gaaattgatt gctgccggca
      181 ttagagaata tccgtacaaa ataatccccc tcttccggga tgataaattc aaatagatgg
      241 gtgctgttgt ttgcacaatt gataaatgat gttgccagtg gagcatcaaa ttcctttggg
      301 gcaaagatgc gctcaagcaa aatgttggca ctgttgcgta tttcatgaat atcgtgacct
      361 gaggcagcaa caagtaagcg cactatttct ctgagtgatg caacatcgta acctttttta
      421 taacgccgtg ccagccgttc aagtgaacgg atggctttta gatttttttc aaaatcaaag
      481 ctgtcgtctg gattacgcgc attgagcatt tttaaaaaac tttcgcggtt gcgggcggta
      541 aatggtcgta tttttttccg ctcggggatt tctttgaatt caacctcaga aagggtggct
      601 atgcgtatga cttcatcaag cagctggtta agggcaacag atacatgagg agggtacacc
      661 tcaatatgtg aatatcctgt ctcaatggct cgctcctcaa cctttaaaag ccagtaaaaa
      721 gcgcgatcat aggtattggt actatctaat gttaaaacat taaaaatatt gagccctaaa
      781 tagatagtgg gaatttcagt ccattgtggt ccctgcccaa catgagaaag tacagcactt
      841 tgtggtgaga catagtacag tgttggccag tttggccttt cttttataag tacaaaacgc
      901 cggctaacaa ttgatgtaaa attaccctgt gctatggaaa catggatttt tgtaatagca
      961 ttgactattc tagtctgcaa ttgaatatcc gcaagtaact tatcggttga ttgggaacta
     1021 ttgctcattg acaaaaccat agcatcacgg atagctaaaa aagaatccct gagtattgca
     1081 gtgtctaccc cttttctaat agcatcaata attgaagaat caacaacaaa ggggaatgcc
     1141 cggtgctcat caaatgcttt ttgtatttcc tgttttacat gttcatcaga aagtttttct
     1201 tcatcctgaa gtgcaaatct acacaggctg ctctgagtgg atgatggtaa tgccatattc
     1261 ccgggttcct ttcttttgtt attgcgatac catttttaaa acaggatatt tttctttgta
     1321 aacaccaagt ggagtataat caatgcttaa tgattttttt ccttcaatgg atgccttaat
     1381 agtgtttatt ttatccatgg ggataaagac gagagcactg ttttcttcat caaacttcat
     1441 aaggcacagc ttgtgttgtg acaatccgta aactaaaagc gaaggaggta tcttataaaa
     1501 cccggatggc tgcatggtat tggtaaatac ttcgccatgc agttggggat attcccgtaa
     1561 aagctgttga aaagtggcag atcgtttttc tggcaataac tccttttcca ccatctgagt
     1621 aaatgatctg gtttcttcta caggtttttg tattattgct gtacttctgg ttttcaaaat
     1681 gtagaagctt tgaatgatat agccactccg cattcttttt ttggattctt cattacacat
     1741 gatagccgga acaataacat cgccaccctt gctgcgtgct atctcttttg ccttttcaat
     1801 acgggcaggt aatgaaacac cgccaatatc ggctatgcca atttcatcaa attgattttt
     1861 atcaacggtg agcaatacct gcacatcgct gtttggtggg agtggagtgt attcattccc
     1921 ggtttttaca aattcaacag atgtgcatga tactgtaaca attaccaaaa ctatacccag
     1981 aaataaaaat ggtagtgcgc tgttataatg ttgtgttgta ttcataatca attccttact
     2041 attgtggtca tttaaatcat agtgaaacag taaaaaatag tcaattgaaa aaaatacttt
     2101 ttgacttttt caataaaact ttaccatgta tcattaattg ttgacatata atctgnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgac tttttcaata aaactttacc
     2281 atgtatcatt aattgttgac atataatctg gccgggatat aggggcaaca ggtgcttttt
     2341 tttagtgcgc cgtactttcc cggtggggaa tgcataatgg cttgatttgt gttcatatat
     2401 agaggatgaa aatatttgat gacagtcatc atggaccatg tcctgaactg tagaacgtca
     2461 tcctgaactg gtttcaggat ctcatggtgg caaaagagat tccggaacag gtccgcaatg
     2521 acaggagcgg caggagagat tccggaaaaa acccggaatg gcagcagagt tacggaaagg
     2581 tgcactgaaa tgtttctaaa aaagaaatac caatctattc ctggagggga ttaatgaaag
     2641 aggttgtcat tgtaagtgcc gtacgaacgc cactgggtaa ttttaatgga gcgctttcat
     2701 ccatgccggc aacacaattg ggtgctatcg tcatagaaga ggcagtgaaa agagcgggca
     2761 tacaaaaaga agatgtggat gaggttatca tggggcaggt tttgccctgc gggtatggac
     2821 aaaatcctgc gcggcaggca gccatcattg ccagcatgcc gtgggaggtg ggatgcctta
     2881 ccatcaataa ggtgtgtggc tcaggattaa aagctgtcat gcttgcagca caggcaatac
     2941 aggctggaga tgctgaggtg atagtggctg gtgggatgga aaacatgagt atggcaccct
     3001 actatttaga aaaagcccgg tttggttatc gtatgggcga tagtcccctg aaagatcaca
     3061 tggttcatga tggattgtgg gacattgtca atgattttca tatggggata tccaatgagt
     3121 tgtgctcaga aaaatatgga atcagcaggc aggagcagga tcgatatgcc gaagcgtcat
     3181 atacgaaagc aatgcatgct attgcatcag gtgcatttaa aaaagagata gtcccggtaa
     3241 ctatcgctca aaaacaacgc acggtagttt ttgatactga tgaatgtcca aaggaaacac
     3301 cgtacgaagt tttagctgcc atgaaaccat cgtttaaagc aggtggggtt acaacagctg
     3361 gcaatagttc ggttatcagt gatggtgctg ctgcactggt ggtgatgtca aaagaaaagg
     3421 cagataagct gggatgcacc atcatggcta ctgttggagc gcaggcaagt gccggtattg
     3481 acatgaagta tgtgctggtg gcgccaatat gggcaatacc aaaatgctgt aaaaaagaag
     3541 gtgtgaacgt ggaagatatt gagctttatg aggttaatga agcgttcagc ggctcaagtg
     3601 ttgcagtgct gaaagaatta aaacttgatg ccggcaaagt caatgttaat ggcggcagcg
     3661 ttgcattggg gcatcctatt ggtgcaagtg gtgcacgggt gctggtgacg cttgtccatg
     3721 aaatgatagc gcgagacaaa aaatcagggc ttgcctctct gtgtctgggt ggaggcgagg
     3781 ctgttgcatt aattgtaaaa cgataactaa aaggataaac gcaatgcagg taaacgatat
     3841 aaaagtattt ggtgttgtag gtgcagggca gatgggtcat ggcatagcct gtgtggcagc
     3901 gttaagcggg cttaccgtta tactgaatga cattagcatg gagtatgtta acaaaggtat
     3961 tcagggtata gatgcagtgt tgacacgaaa tgttgataag ggcacattga cacatgagga
     4021 aaaagatgca gtgctgcata ggattaaacc gtgcacagca ttgaatgacc ttgcagatgc
     4081 agattttatt gttgaagctg ccagcgaaaa tcctcaaata aaatggaaac tatttagcga
     4141 tttagacgcg ctatgcccgt cgcatgttgt attagctacc aatacctcat ctatccccat
     4201 tggacgtatt gcatcggtaa ccagacgggc tgacaaagtt attggcatgc actttatgaa
     4261 tcctgtgccg gtaatgaagc tggttgaggt aataagggca cttgccacat ctgatgaaac
     4321 ctttgatgta acatggaatt taagcataaa gtttggtaaa acgccagcat tggctaatga
     4381 ttatcccggc tttattgcca acaggatact tatgcccatg ataaatgagg caatctatag
     4441 cctttatgaa ggggtaggga cacgcgaatc aatagacact gtcatgaagt tagggatgaa
     4501 ccatcccatg gggccgcttg cactggcaga ccttattggg cttgatacct gccttgctat
     4561 tatggaaacc ctgtatgaag gatttaagga tacaaaatac cgtccatgtc cattactacg
     4621 aaggtatgtt gaagcagggt ggttggggtg taaaaccaaa aaaggttttt atgattatac
     4681 atagtataag ggtaatagtc catgcacttt gaattatcag aagaacagat aatcattcag
     4741 gaaactgcaa gccgttttgc aaaacaggaa ctggaacctg cagcagccgt tctggatgca
     4801 accaaaaaca gagaaatttt aaaatcaaat ttaaaaaagc tggcaaagct tgggtttaac
     4861 ggaatagcag tagacccccg ctatggcgga acaggtgccg gcagtgttgc attttcactg
     4921 gtgctgacac agctggggaa agcatgtgca gctacagccg ttacaacatc agtgacgaac
     4981 atggtggcag aggttattca gtcagtgggt acagaagagc aaaaat
//
LOCUS       DAOSOX010000169         5014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16874_length_5014_cov_7.077637, whole genome shotgun sequence.
ACCESSION   DAOSOX010000169 DAOSOX010000000
VERSION     DAOSOX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16874_length_5014_cov_7.077637"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLX22_10235"
     CDS             complement(<1..281)
                     /locus_tag="PLX22_10235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT20316.1"
                     /translation="MKVIACLGNPGKKYSNNRHNAGILVGAAIAQHYSITVGKKEHDA
                     LTGSGIIEGHECIFMIPQTYMNNSGIAVQKVMSYYKELPDNLIVLHDDI"
     gene            complement(289..630)
                     /locus_tag="PLX22_10240"
     CDS             complement(289..630)
                     /locus_tag="PLX22_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT20317.1"
                     /translation="MKITSTKINGVVLIKVEGSLATEQIKQFEKELYTALDKNSNVII
                     DFNELSFICSAGLSLLIASHKKAEEKKLRLVLTGCSDDILKLFSLTEIDQHLNIVNSI
                     EEAQSLINAMK"
     gene            897..1850
                     /locus_tag="PLX22_10245"
     CDS             897..1850
                     /locus_tag="PLX22_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT20318.1"
                     /translation="MRAARIIAPRKFTVDNDPIPDCSDSHVRVKLLKAAICGSDIPYF
                     TSVYSRESYPLPPGYPGHECLGVIEASRVKQFKEGTPVMYYPPFLDGFKEYHVTPPSR
                     VQSIPDTLPLNELLITQLLGAVSHCAFRIDKPYNKHVAIMGQGPVGLLFTALMKNFGA
                     RSVIAIDPLDYRCKAAINMGATHTINPHTSDLLEAIKAITDGSMADIVIDAYGQDVAV
                     INKCFEIARHNGQVAFFGICLEEAPRLNFNTFFRKELRMIASVGPDLTMDYPYALDMI
                     LKGAIDVTPLLTHDIPFEDIQKGFEMASNREDGAIKIVLSF"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1979..2164
                     /locus_tag="PLX22_10250"
     CDS             <1979..2164
                     /locus_tag="PLX22_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20319.1"
                     /translation="KAFLLCLVTIALAVWFVYGLYHHSYLAIAIPAACITLVILGMIF
                     WVGIAIITTRSTLKKDK"
     gene            2171..3409
                     /locus_tag="PLX22_10255"
     CDS             2171..3409
                     /locus_tag="PLX22_10255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT20320.1"
                     /translation="MRALFLCYRGNPFCGGQGIYLYNLTKELAKLGVVIDVIVGPPYP
                     DPLDEWATVYKLENLNMWAIKTKHFPYEKLIKLYKPWHFVDYCLTRFHIFPEMETFSM
                     KAFFLLRKLLKTNHYDIIHDVQCLGWGLLPMKGYGIPIVSTVHHPLTKDREADFLVDN
                     TVWEMACTILFYPLTMQRIVINRLDRVITSSKEGINALNEAFHLSPEKVSVVYNGMDV
                     EFFRNNGSPRQENTLLFVGNTEDHKKGLRYLFDAMTMLPEHVTLTIVDDGPPKRLTAW
                     GWITERGLEKRVTFTGKVGLSTLLHLYSTSTILVMASLYEGFGLPAAEAMACNTPVVV
                     TSAGALPEVVGDCGVIVPPKDAVALKEGILKLINNKALREELGKKGRKRVEDNFAWPV
                     AAKNTLAVYHDVIQSYRSRI"
     gene            3406..4638
                     /locus_tag="PLX22_10260"
     CDS             3406..4638
                     /locus_tag="PLX22_10260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOT20321.1"
                     /translation="MKICLMSYRGNPYCGGQGIYLMYLARELAKLGHEVHAIVGPPYP
                     FPVEGVTYHYVSNHNYFNIKDFVKPHKPFATFNPLNFYEFVASKLGIFPEIESFSFRA
                     YSKLRQLLKTHSFDVIHDNQCLGYGFLLIKQFGIPFISTIHHPLTIDRSTWFEYPSSF
                     FLKTKRILYYPLVMQKYVANTMDLIVTVSHDSAQEIYNAFGVPLEKQRVVYNGMDSSI
                     FNPVKLPKRHNSLIFVGNVEDRKKGVIYLLKALTLTRNKVHLTIVDGGAPNRNYVPRW
                     IDKFNLNEQITFTGKIPLDKLVQLYAQTQIALCPSLYEGFGFPAAEAMACELPVIAST
                     GGALPEVVGQHMKTGILVPPRDPYALAEAIDFLIENPDIRNRIGKAARQRVLKTFTWE
                     NAARQMVNVYQEAIDAYR"
     gene            4625..>5014
                     /locus_tag="PLX22_10265"
     CDS             4625..>5014
                     /locus_tag="PLX22_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT20322.1"
                     /translation="MLTVDFDLLAVKDNNIALDAGCGLGRHSFEFVSRGANVFSMDMD
                     MDCVRRTRFTLVYMRDNGQAKQGTHFQVLLGNALKLPFRDNTFDRVICAEVMEHVPDD
                     NQACHELARVLKPGGLIAITVPTYISEM"
BASE COUNT         1443 a          975 c          989 g         1507 t
ORIGIN      
        1 tctatatcat catgcagaac aattaaatta tcaggcaatt ctttgtaata tgacatcacc
       61 ttttgcactg ctatgccgct gttattcatg tacgtttgcg gtatcatgaa aatacattca
      121 tgaccttcga ttataccgct tccggttagt gcatcatgct ctttctttcc tactgttata
      181 ctgtagtgct gcgctatagc agcacccaca agtataccgg cattatgacg attgttagaa
      241 tatttttttc cgggatttcc caggcaggct attactttca tttgctaatt atttcattgc
      301 attaatcagt gactgcgctt cttctattga gtttacaata ttgagatgtt gatcaatttc
      361 agttaacgag aataatttta atatatcatc actgcacccg gttaaaacaa gccttaattt
      421 tttttcctct gcctttttgt gtgatgcaat gagcaaactt aaaccagcac tacatataaa
      481 cgatagttca ttaaaatcga ttattacatt gctattttta tcaagtgccg tatatagttc
      541 tttttcaaac tgcttgattt gttctgtagc aagcgaaccc tcaactttta tcaataccac
      601 cccatttatc tttgttgatg tgatcttcat tcccaaaccc ctgaagctac tatataataa
      661 actataggca tatatcctaa aacataaata ataattgtca agtaaaatca ttatatcata
      721 ggtacaacta tttaaaaaat ttttaagtta aacaacaatg aaccactata tacatgttaa
      781 ctattcaaat acgctttaaa atgtcaataa atagattgac aataaccata tgaaataaca
      841 ttgtgtagtt atgatttatt tgtatatatt aatttatttt atcaggattt cactctatgc
      901 gtgcagccag gattattgct cccagaaaat ttacagttga taatgatcct atccccgatt
      961 gttctgacag tcatgtcagg gttaagttat taaaggcagc gatatgtggt tcggacatac
     1021 cctattttac atcagtttat tccagagaat cctacccgct gccacctgga tatcccggcc
     1081 atgaatgtct gggtgttata gaagcatcca gggttaagca gtttaaagaa ggaactcctg
     1141 taatgtatta tccgccattc cttgacggat ttaaagaata ccatgtaacg ccaccatcac
     1201 gagtgcagtc aatccctgac accttacctt tgaatgagct tttgataaca cagcttttag
     1261 gtgcagtatc acattgtgcc ttcaggattg acaaaccata caacaaacat gtagccatca
     1321 tggggcaggg gccggtagga ttgctattta cagcattgat gaaaaatttt ggggcacgga
     1381 gtgttattgc tattgaccca ttagactacc gttgtaaggc tgcaataaac atgggtgcaa
     1441 cccataccat aaatccccat accagcgacc ttttagaagc aatcaaagct attactgatg
     1501 gttcaatggc tgatattgtt attgacgcat acggacagga tgtggcagtt attaataaat
     1561 gctttgagat agcccgtcac aatggtcagg tagctttttt tggaatatgt ctggaagaag
     1621 ctccccgcct taacttcaat acctttttca gaaaagagct gcgcatgatt gcttctgttg
     1681 ggcccgacct gacaatggat tatccctacg cactggatat gattttaaaa ggtgctattg
     1741 atgttacacc attattaaca cacgatatcc cttttgaaga tattcaaaaa ggttttgaaa
     1801 tggcaagcaa tagagaggac ggcgcaataa aaattgtatt aagcttttaa caataacgag
     1861 ttataacaga cgaggtacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1981 agcatttttg ttatgtttgg ttactatcgc acttgctgtg tggtttgtat atgggttata
     2041 tcatcattct tatttagcca ttgctattcc ggctgcatgc ataacgcttg ttatacttgg
     2101 catgattttc tgggttggta ttgctataat tactacacga tcaactttaa agaaagataa
     2161 atagcaattt atgcgtgcat tatttctgtg ttaccgtggc aacccttttt gtgggggaca
     2221 gggtatatat ttatacaatc tcacaaagga attagcaaaa ttaggcgttg taattgatgt
     2281 tattgtagga cctccctacc ctgacccact tgatgaatgg gcgacagtat ataagctgga
     2341 aaatttaaat atgtgggcaa tcaaaacaaa acatttccca tatgaaaagc taattaaact
     2401 gtataagcca tggcattttg tagattattg tttaacccgt tttcacatat tccctgaaat
     2461 ggaaaccttc agcatgaaag cgttcttttt actgcgaaag cttttaaaga caaaccatta
     2521 cgatattatt cacgatgtac agtgtttagg gtggggatta ttacccatga aaggttacgg
     2581 cattcccatt gtatccacgg tacatcatcc tttaacaaag gatcgcgagg cagattttct
     2641 tgtggacaat accgtatggg agatggcatg taccatactc ttttaccctt taaccatgca
     2701 gcgtatcgtt ataaatcggc tggacagggt aataacctca tcaaaagaag gcattaatgc
     2761 actcaatgaa gctttccacc tgtcacctga aaaggtatcg gttgtttata atgggatgga
     2821 tgttgaattt ttccgtaata atggtagccc ccgtcaggaa aatacattgc tttttgttgg
     2881 caataccgaa gaccacaaaa aagggttgcg ttatcttttt gatgccatga cgatgttacc
     2941 tgaacatgtt acacttacca tagttgatga tggaccccct aaacgtttaa cagcatgggg
     3001 ttggataaca gagcgtggtc ttgaaaagcg ggtaaccttt accggtaagg tgggattatc
     3061 tacattactt catttgtaca gtaccagcac tattcttgtc atggcttcgt tgtacgaagg
     3121 atttggatta cctgctgcag aggcaatggc atgtaatacg cccgtggtgg taaccagcgc
     3181 aggggcatta cccgaagtag ttggcgattg tggcgtgata gtaccaccca aagatgctgt
     3241 tgcattaaaa gaaggtattt tgaaacttat taataataaa gcactccgcg aagaattagg
     3301 caaaaaagga aggaaacgcg ttgaggataa cttcgcatgg cctgttgccg caaaaaatac
     3361 gcttgctgtg tatcatgatg ttatccagtc atacaggagc aggatatgaa aatatgttta
     3421 atgagttatc gtggcaatcc gtattgcggt ggtcagggga tttacctgat gtacctggcg
     3481 cgcgaactgg caaaattagg acatgaggtc catgctattg ttggtccacc ttatccattt
     3541 cccgttgaag gtgtaacata ccattatgtc agtaaccaca attattttaa cataaaggat
     3601 tttgttaaac cacacaagcc atttgccact tttaaccctt taaactttta cgaatttgta
     3661 gcatcaaagc ttggcatatt ccccgagata gaatcatttt cattcagagc gtatagcaaa
     3721 ttgcgacagt tgcttaaaac tcactcattt gatgtcatcc atgataatca gtgtctggga
     3781 tatggatttt tgcttataaa gcagtttggc attcctttta tatccaccat acatcacccg
     3841 cttactatcg accgttcaac atggtttgaa tatccttcaa gcttcttcct taaaacaaag
     3901 cgcatccttt attacccgct tgtgatgcaa aaatatgtag caaataccat ggaccttatt
     3961 gtaacggtgt cgcacgactc cgcacaggag atatacaacg catttggagt gccacttgaa
     4021 aaacagcgtg ttgtgtacaa tggcatggac agctccatat ttaatcctgt aaaacttcct
     4081 aaaaggcaca acagccttat atttgttggt aatgtagagg accgcaaaaa aggtgtcata
     4141 tatttgctca aagctttaac gcttacccgt aataaggttc atttaaccat tgttgacgga
     4201 ggagcaccca accgaaacta tgtcccacgt tggattgata aatttaatct taatgaacaa
     4261 ataaccttta ccgggaagat accactggat aaactggttc aactgtatgc acaaacacag
     4321 atagcactgt gcccttcctt atacgaggga tttggatttc cggcagcaga agccatggct
     4381 tgtgagttgc cggtgattgc aagcacaggt ggtgctcttc cagaagttgt tggacaacac
     4441 atgaaaacag gaatactggt gccaccacgc gacccgtatg cattagccga ggctatagac
     4501 tttttaatag aaaatcctga tatacggaac cgtataggga aagctgcacg ccagcgggta
     4561 ttaaaaactt tcacatggga aaatgctgcc agacagatgg taaacgtata tcaggaggca
     4621 attgatgctt accgttgatt ttgatctcct tgctgttaag gataacaaca ttgcgcttga
     4681 tgcaggctgt ggtctggggc gccattcctt tgaatttgta tcacgaggcg ccaatgtttt
     4741 ttccatggat atggatatgg attgcgtgcg gcgcactcgc tttacgttag tgtacatgcg
     4801 cgacaatggg caggcaaagc agggtaccca ttttcaggta ttgttgggga atgcgctgaa
     4861 gctccctttc agggataaca cctttgaccg cgttatctgt gccgaggtca tggaacatgt
     4921 gcctgatgat aatcaggcat gtcatgagct ggcacgagtg ttaaagccag gcgggctgat
     4981 tgccattacc gttccaacct atatttccga aatg
//
LOCUS       DAOSOX010000170         4997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16942_length_4997_cov_8.312626, whole genome shotgun sequence.
ACCESSION   DAOSOX010000170 DAOSOX010000000
VERSION     DAOSOX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16942_length_4997_cov_8.312626"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLX22_10270"
     CDS             <1..933
                     /locus_tag="PLX22_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20323.1"
                     /translation="KQLLTFSKGGEPIKAVASLEQLITDTAQFVFSGSNVRCHLDIQP
                     ELWNCEIDAGQMGQVFQNIMINAMQSMPQGGIVTIKAYNYVYDGKAQIPLLPGDYIAI
                     TIKDEGIGIPEENLSKIFDPFFTTKEHGSGLGLATSYSIIKRHGGYIEVTSDVGKGSE
                     FIIYLHATHETNEEKKEGSAIINSYEGKILLMDDDFTIYEVVSHMLDMYGFTVDWVES
                     GEKAIAQYKKSLDEKRPYDIIIMDLTVPGAMGGAQATKEILRINPDAKVIVSSGYSND
                     PIMSHYKEYGFKGVIAKPYRIEEIMNVINDLMKQ"
     gene            1075..1767
                     /locus_tag="PLX22_10275"
     CDS             1075..1767
                     /locus_tag="PLX22_10275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT20324.1"
                     /translation="MKKQCIVFIGIAALLTINASVVKQKQSDSAKVKEQYSNDIKRKE
                     DIQKKIYDLMKEINAHAGGDALIVPVDPEKADNVVDHDDYQKKEAGEKKADDKKTDDT
                     ALIAPTKGKTTSKYGYRQNPVTKKYAFHKGIDIAAPKGTKVVASAGGVVATSNYLNNG
                     YGNCVIIEHANNVKTLYAHLAQRTVAAGDTVIQGQKIGTVGATGRATGPHLHYEVYKG
                     GKSINPEDLVHY"
     gene            1785..2423
                     /locus_tag="PLX22_10280"
     CDS             1785..2423
                     /locus_tag="PLX22_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOT20325.1"
                     /translation="MNKKACILGATGLVGGELLSLLLHDDYYAHITIIVRKKIAIPSQ
                     KVAVFVTDFANLEKVVEAFQVDDVFCCLGTTMKKAGSKDAFKQVDYEYVLKAAAIAHK
                     QGAQQFLVISAVGANPHAYAFYNRIKGLMKKDVSSIPLQTIHIFRPSLLVGKRQEKRL
                     LEDIARLFAKPMQFLLKGSFAKYAPVEARTVAEAMAGAAKLNTKGVHIHEAL"
     gene            2669..3559
                     /locus_tag="PLX22_10285"
     CDS             2669..3559
                     /locus_tag="PLX22_10285"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOT20326.1"
                     /translation="MTGNDIKTVLIIGSGTMGQQIGFVCALHGCHVHMYDVHEEALKS
                     AQNRLTQLAQWFEKHTVKNTTNALSLIRYYTDPQQAAIDVDFVSESVPENPELKAKVF
                     STFHALCREQTIFTTNTSSLVPSQIAAATGRPDRFLAFHFHDIRTTKIVDVMPHAGTS
                     QEVINATVEFAKKIGQIPIVLKKENNGYVFNTMLMTLLASALTLASNGVADIHDIDKS
                     FMGVMGTPIGPFGIMDSIGLDTVHEITKYWADLLDDKQGKKNAALLQQYVEKGLLGTK
                     TNKGFYTYPHPEFSKPDFLQ"
     gene            3761..4894
                     /gene="tgt"
                     /locus_tag="PLX22_10290"
     CDS             3761..4894
                     /gene="tgt"
                     /locus_tag="PLX22_10290"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT20327.1"
                     /translation="MRFEILQHDSNCNARLGRIHTPHGMVHTPVFMPVATRGAIRALT
                     FRDIEELGFEIVLSNTYHMYIRPGTNLLQKAGGLHNFMNYHKPLLTDSGGFQVFSLAD
                     LRKVHQDGVEFQSHIDGSKHFFTPEKVLDIQRIIGSDIMMVLDECVAYPADYQTAKTA
                     VDRTVQWAGRSYEYWHNNFDIEAQALFAIVQGGVYPDLRLQCSTELQQYNFSGFAVGG
                     LSVGEPKSQYSDITAVTVPALPWDKPRYMMGVGSPLEILEAIRYGVDMFDCVMPTRIA
                     RNGTLFTTNGRINIKSGIYAEDFNPLDEHCHCYVCKTFTRAYLRHIYKMNEIASLIYN
                     TYHNLYFMKYFMDTIQKSIANNQFMDEYNRWKKIYNNSEINTM"
BASE COUNT         1635 a          864 c         1093 g         1405 t
ORIGIN      
        1 aagcagcttt taaccttttc aaaaggtggc gagccaataa aagcggttgc ttcactggaa
       61 cagttgatta ccgatacagc acagtttgtt ttttcaggct caaatgtacg gtgccatctt
      121 gatattcagc ctgaactatg gaactgcgaa atagatgccg ggcagatggg tcaggttttt
      181 caaaacatca tgataaatgc catgcaatcc atgccgcagg gtggcattgt tacaattaaa
      241 gcatataatt atgtctacga tggcaaagcc cagataccac ttttaccagg ggattatatt
      301 gccataacca ttaaggatga aggtataggt ataccagaag aaaatttgtc caaaatattt
      361 gatccatttt ttaccacaaa agagcacggc agcggattgg gacttgcaac aagctattct
      421 ataatcaagc ggcatggagg ttatattgag gttacttccg atgtagggaa aggttcggaa
      481 tttattatct atttgcatgc aacccatgaa acaaatgaag aaaaaaagga aggatcggca
      541 atcattaatt catatgaagg taaaatttta ttgatggatg atgattttac catctatgag
      601 gtagtttcac atatgctgga tatgtacgga tttacagttg attgggtaga atctggtgaa
      661 aaagcaattg ctcagtataa aaaatcgtta gatgaaaaaa ggccctatga tatcatcatc
      721 atggatttaa cggtgcccgg cgctatggga ggagcgcagg ctacaaaaga aattctaagg
      781 attaatcctg atgcaaaggt tatcgtatca agcggttatt ccaatgatcc aataatgtcc
      841 cattacaaag aatatggatt taaaggcgtt attgccaaac cataccgcat agaagagatc
      901 atgaatgtaa taaatgatct catgaagcag taacaattgt atgcaatcca tccgataact
      961 atagtgtaca acggcagtta tcatttttac tggcgatagt tttagaggat aaaggtatag
     1021 tatgatgaac tatcgcacac aggtaaataa aacattgtgg gataggaaca cagtatgaaa
     1081 aaacaatgca ttgtatttat tggtattgca gccttactga caataaatgc ctcggtggta
     1141 aaacaaaaac aatcggattc tgcaaaggta aaggagcagt acagcaatga cataaagagg
     1201 aaagaagata tccagaaaaa gatatatgat ttaatgaaag agatcaatgc acatgcaggc
     1261 ggtgatgcat tgatagtgcc ggttgatcct gagaaggcag acaatgttgt tgaccatgat
     1321 gactatcaaa aaaaagaagc aggtgaaaaa aaggctgatg acaaaaaaac tgatgataca
     1381 gcattgatag caccaacaaa aggcaaaacc acaagcaaat atggataccg gcagaatcct
     1441 gttaccaaaa aatatgcatt ccacaagggg attgatattg ctgcaccaaa gggtacaaag
     1501 gttgtagcaa gtgctggtgg cgttgttgca acatcaaact atttaaacaa tgggtatggc
     1561 aattgtgtta ttattgagca tgcaaacaac gtaaaaacac tctatgcaca tcttgcgcag
     1621 agaacagtag ctgctggcga tacagtcatt cagggacaaa agattgggac ggtaggtgct
     1681 acaggaaggg ctaccggccc gcatctgcac tatgaggtat ataaaggcgg aaaatcaata
     1741 aatcctgagg atctggtaca ctactaagaa ggtgtgttga ttatatgaat aaaaaagcgt
     1801 gtatactggg tgccaccggg ttggtaggtg gggaattact ttctttacta ctccatgatg
     1861 attattatgc acatatcact atcatcgtac gaaaaaagat tgcaatacct tcacaaaagg
     1921 ttgctgtctt tgttactgat tttgccaatc tggaaaaggt ggtggaagca tttcaagttg
     1981 atgatgtatt ttgctgtctt ggtaccacca tgaaaaaagc aggctcaaag gatgccttca
     2041 aacaggtaga ttatgagtat gtgctgaaag ctgcagcaat tgcccataaa cagggtgcac
     2101 aacaattttt agttataagc gctgttggtg ccaaccccca tgcttatgca ttttacaaca
     2161 ggataaaagg gttgatgaag aaggatgtgt catcaatccc cctgcagaca attcacattt
     2221 tcaggccttc gcttttagtg ggtaaaaggc aggagaagcg cctgcttgag gatatagccc
     2281 gattgtttgc aaagcccatg cagtttttgc taaaaggaag ctttgcaaag tatgcccctg
     2341 tcgaagcccg tactgttgca gaagcaatgg ctggtgcagc aaagttgaat acgaaaggag
     2401 ttcatatcca cgaagcatta taggcgatag ttattgtgca ggtgcttttt tgttgcataa
     2461 aaatattgtt actgtagtga tgaatgatgg cagtgttgcc attcatgagt gacagagata
     2521 tagccgcatg agatggagaa agcggttttt tatttttaca gtgcattaat gtattgacga
     2581 acatacaggc ataattgtta tccctccaca ttgaactgtt atagagtaac tatcgtacac
     2641 aaataattat taattattgg aggattgtat gacgggtaat gacattaaaa cagtattgat
     2701 cattgggtcg ggtaccatgg gacagcagat tggttttgtg tgtgccctcc atggatgtca
     2761 tgtgcacatg tacgatgtcc acgaagaagc attgaagagt gcacagaata ggctgacaca
     2821 gttagcacag tggtttgaaa agcatactgt taaaaatact actaatgctc tgtcccttat
     2881 acgctattat actgatccac aacaggctgc tattgatgtt gattttgtca gcgaatcagt
     2941 gcccgaaaac ccagaattaa aagctaaagt tttttcaaca tttcatgcac tgtgccgtga
     3001 gcaaacaata tttaccacca atacctcatc gcttgttcca tcacagattg ctgccgctac
     3061 aggaaggccc gacagatttt tagcatttca cttccatgat atcaggacca caaagatagt
     3121 tgatgttatg ccgcatgcgg gaacctcgca agaggtaatc aatgcaactg ttgaatttgc
     3181 aaaaaaaata ggtcagatac caatcgttct taaaaaagaa aacaatggct atgtttttaa
     3241 taccatgctc atgacattac ttgcctcggc attgaccctg gcttctaatg gtgtggctga
     3301 tatccatgat attgataaaa gctttatggg cgttatgggc acaccaattg gaccatttgg
     3361 tattatggat agcatcggat tggatacggt acatgaaatt acaaaatact gggcagattt
     3421 gcttgatgac aaacagggca aaaaaaatgc tgcattgtta cagcagtatg ttgaaaaagg
     3481 cttattaggc actaaaacca acaaaggttt ttacacctat ccgcatccag aattttccaa
     3541 accggatttt ttgcagtaat ggcttgtaat gtttgatttt gttgagccct ctatagctgt
     3601 gcccagcaag agtacacggc aatttttggc gatagtaact cttgaatgaa attagcagtg
     3661 catagtagtt ttattgtgat gaaacaaaaa atagtattgt ttattccatg attttgtgat
     3721 gctatggcaa tggtgataga atgtcactga taggtatggt atgcgatttg aaatactaca
     3781 acacgatagc aattgcaatg cacgtctggg gcggatacac actcctcatg ggatggtgca
     3841 tacaccggta ttcatgcctg ttgccacgcg cggagcaatt cgtgcattga cattccgtga
     3901 cattgaagaa ttaggctttg agatagttct ttcaaatacc taccatatgt acatacgccc
     3961 cggcaccaat cttttacaaa aagcgggcgg gttgcataat ttcatgaact atcacaaacc
     4021 tctattaact gactcagggg gattccaggt tttttcactg gctgacttac ggaaggtgca
     4081 ccaggatggc gttgaatttc aatcacatat tgatggctca aagcattttt ttactcctga
     4141 gaaggtgctg gatattcaaa ggattatagg ttcagatatc atgatggtgc tggatgaatg
     4201 tgttgcctat cctgctgact atcaaacagc taaaacagca gtagacagaa cagtgcagtg
     4261 ggctggacgg tcatacgaat actggcataa caattttgat attgaagcac aggctctttt
     4321 tgccatagta cagggtgggg tgtatcctga tttgcggtta cagtgctcaa ctgaattgca
     4381 acagtataac ttttcaggat ttgctgttgg aggactttca gtaggcgaac caaaatctca
     4441 atatagtgac attactgctg taacagtgcc ggcgctgcca tgggataagc cgcgctatat
     4501 gatgggagtt ggcagcccac tcgaaattct tgaagcaatc aggtatggtg ttgatatgtt
     4561 tgattgtgtt atgccaactc gaatagcacg caatggtacg ctttttacta ctaacggcag
     4621 gataaacata aagtcaggta tctatgctga agattttaat ccgttagatg aacattgtca
     4681 ttgttatgtg tgtaaaacat ttacacgtgc atatttgcgc catatttata aaatgaatga
     4741 aatagcttct ctgatttata atacctatca taacctttat tttatgaaat attttatgga
     4801 taccatacag aaaagtatag caaacaatca atttatggat gaatataacc gatggaagaa
     4861 aatatataac aatagtgaaa taaatactat gtaaatagta aaacaacagg ataataacct
     4921 gaatcaaaag taaatttttc tggaattatt attgtgtata aataaaaaaa attatttaca
     4981 aaacaaaaaa aataaaa
//
LOCUS       DAOSOX010000171         4994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_16952_length_4994_cov_9.382871, whole genome shotgun sequence.
ACCESSION   DAOSOX010000171 DAOSOX010000000
VERSION     DAOSOX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16952_length_4994_cov_9.382871"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLX22_10295"
     CDS             complement(<1..447)
                     /locus_tag="PLX22_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOT20328.1"
                     /translation="MIHNNVKTDFISEEYSPQVDTSAYLHPMAAVIGHVIIGKRVMVA
                     PFAVVRGDEGQPIHVGDDSNIQDGVVIHALETEHNGHPINNNLVEVDSKKYAVYVGKR
                     VSLAHQSQIHGPAYVGDSTFIGMQSLIFKAKVGNNCVVEPKSLVLGV"
     gene            908..3202
                     /locus_tag="PLX22_10300"
     CDS             908..3202
                     /locus_tag="PLX22_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT20329.1"
                     /translation="MFKQLLHRYSIHQVTLLSTESPLHQRIVRYLTRNRIEFSTITSF
                     NDSADTSLLILPLSMHTITPVSDILQQMLQLDHTPLLVFATDFPVTTRFKKRFSIAGI
                     IGILELTEHPRFITVESHTTQSNPTASLNALIYTTENLISRSIYKILSQNILYNALIK
                     LHPSIICITTIEGTIIYFRANREYLQRNNNIKKILYGKKIYDFATNPDSINNLFNLIN
                     KNIEQRETEITFCIDNNTFNLLVTGEKIQLYNDFNAYLLIGINITTQRKTEDLLISSE
                     LKYRTLVENLPDGLSLSKGEMLLYANPQFFKLFHYPPDTLMTDIDFHVNIHPEDWDTL
                     FPLLHQSLDGVTNLSIRGITNDEKQILLELHILSFQIDNSLYHQIYWQDITRTQKLLD
                     TILHSERLSAIGELASGITHEFNNILTSIQGYIQYTINNPDDAEANKKAFKVIEKMTE
                     QGHTILKNLSMLSRKDITNKERHSVAELLQDILKIQEKILTKDNITVVRKFFKDPVVY
                     VDPGLIQQVFLNILLNAVHAIRPKGKGTITITTDESNGNAIIKIHDTGTGIDPKIKDE
                     IFNPFFTTKQGGAYNIVGTGMGLSISKNIIEQHSGSIYFYSIPEKETEFIIELPKFSS
                     AYLPNEIKISCEPRDIANLSVLIVDDDEAIRDLFKTLLQSLGITTISFATNGYEGYSL
                     CKDSNFDIVFMDVSMPVLSGIDAYKMIKDEKPQQKVVFITGIFYEDQIKELVDKENAY
                     GYIKKPFDIKEIKALLYSIASCRR"
     gene            3227..4093
                     /gene="mtnP"
                     /locus_tag="PLX22_10305"
     CDS             3227..4093
                     /gene="mtnP"
                     /locus_tag="PLX22_10305"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOT20330.1"
                     /translation="MKTTIDAGIIGGSGLYQIDGVTIVDEVSVKTPWGMPSDIITIAE
                     IDGVRAGFLPRHGRGHFILPHEINYRANIAALKMLGAGQIIAFSAVGSLKDRIKPLDF
                     VIPDQIIDRTKSRISTFFGDGIVAHIAFADPFCSRLHELILVAAQQLNIPMHTNEILI
                     CMEGPAFSTRAESHLYRSWQAGVINMSTLPEAKLAREAEMCYAVICMSTDYDCWKEDE
                     EHVTVDMVVNNLNKNAANAKALIKHLVPLLKQERTCACKEAIKYAIMTDSTKQPQKQK
                     KKLKAILPHYFS"
     gene            complement(4081..>4994)
                     /locus_tag="PLX22_10310"
     CDS             complement(4081..>4994)
                     /locus_tag="PLX22_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT20331.1"
                     /translation="LAAKLHFSINKKTLEAIPQMLDVASKISVERVRDEIKKMLEVDK
                     PSVGFEYMRVTGLLDLCLPELSQGFGMQQNKYHLYDIYYHSIYSCDAAPNDKPLIRLA
                     ALFHDIGKITTRREGHDGDYTFYNHEIIGAKIARRIMRRLKFSNDEIEYVTHLVENHM
                     FYYTQDWTDGAARRFIRKVGLHNLEDLFILRLADRVGSGMRDGLPEPIKYLKKHIEKV
                     IAKDNAFTVKDLDIDGYSIMDALHIKPGPMVGKILNELLEKVLDNPELNNYQTLLTLA
                     KEIYAIMERSDESSCHDSIQHQQHYEK"
BASE COUNT         1640 a          978 c          843 g         1533 t
ORIGIN      
        1 cacccccaga accagtgatt taggctctac tacacaattg ttccccacct ttgccttgaa
       61 tattaacgat tgcatcccta taaaggtact atcgccaaca tacgccgggc cgtgaatctg
      121 tgactgatgt gcaagtgaaa cacgtttccc aacatataca gcatattttt tactatctac
      181 ctcaactaaa ttgttattga ttggatgccc attatgctct gtttcaaggg catgaatgac
      241 gaccccatcc tgaatattgg aatcatctcc tacatgaatt ggttgccctt catcaccacg
      301 cactacagca aatggtgcca ccatcacccg tttaccaatg ataacatgtc caatgacggc
      361 agccattgga tgcaaatatg ccgaggtatc cacctgtgga gaatattctt cactgatgaa
      421 gtctgttttt acattgttat gaatcataga ccaatcctcc ataaaaaata tattgttttt
      481 tgaaaggtta tcgatattat atttctgtca agattaatca caatgacatg aataaaaaag
      541 cgttgatgaa taactattat tgttagtatt gtgtactcat tgagaagtta ctcatggctg
      601 gggtgtaacc catagatgat gtctatggaa taatacttta attgtaaggc aaagccgata
      661 gttattgcct atggtatgca tcgtcgcagc aaagaatcta ctttttcata acagagattc
      721 tttgggactg cgcacatgca aatgacaatg atcgtagggg attttttagc cgatgtactc
      781 cctatgcttg acaatgcttg gcaatgatta acaatgctat ggtggtgtta gtgatggcat
      841 tactatattt aatttaagtg aacctttatc aggtttttca tcattaaaaa attaataaca
      901 aaatattatg tttaaacaat tacttcatag gtactctatc catcaggtaa ctctgttatc
      961 aacggagtca ccattgcatc agcgtatagt tcgctattta acccgaaatc gcatagaatt
     1021 ttctacaatt acatcgttca atgatagtgc tgacacttct ctgttaattt tgccgttgag
     1081 catgcatacc ataacacctg tttctgatat attacaacaa atgttgcaat tggatcatac
     1141 accgctattg gtttttgcta ctgattttcc tgtaacaacg cgcttcaaaa aaaggttcag
     1201 tatagctggt ataatcggca tactggagct aaccgaacat cctcgtttta ttacagtaga
     1261 atcgcatacc acacaatcca atccaacggc ttcgttaaat gccttaatat acaccacaga
     1321 aaaccttatt tcacgcagta tctataaaat attatcacag aacattttat ataatgcatt
     1381 gatcaaatta cacccttcta ttatctgtat tactaccatt gagggaacaa taatatattt
     1441 tagagcaaat agagagtatt tacagcgcaa taataatatt aaaaagatac tctatggaaa
     1501 aaaaatttat gattttgcaa ctaatcctga ttccataaat aaccttttta acctcataaa
     1561 taaaaatatt gagcagcgcg aaactgaaat aactttctgt attgataaca atacattcaa
     1621 cctgcttgtc accggtgaaa aaatacaact ctataatgat tttaatgcct atttattaat
     1681 tggtataaac atcacaacgc aaagaaaaac tgaggacctg cttatttcat cagaattgaa
     1741 ataccgtacg ctcgtggaaa accttcctga tgggttatcc ctgtctaaag gtgaaatgtt
     1801 actgtatgcc aatcctcagt tttttaaatt atttcattat ccacccgata cccttatgac
     1861 tgatatcgat ttccatgtaa acattcaccc ggaagactgg gatacacttt tcccattgct
     1921 ccatcaatca cttgatggtg taacaaatct ttcaattaga ggtattacta acgatgaaaa
     1981 acaaatacta ttagaattac atatattgag ttttcagata gataattcat tatatcatca
     2041 aatatactgg caagatataa cccggacaca aaagctatta gatacaatac ttcattctga
     2101 gcgtctttca gccattggcg aattggcaag tggtatcact catgaattta ataacatcct
     2161 taccagcata cagggctata tacaatatac cattaataat cctgatgatg ccgaagctaa
     2221 taaaaaagct tttaaggtta ttgaaaagat gacggagcag ggacacacca ttttaaaaaa
     2281 tctatcaatg ctatcacgaa aagatataac caataaagaa cgccatagcg ttgcagaatt
     2341 attacaggat atattaaaga ttcaggaaaa aattttaacc aaagataata taacagtagt
     2401 aagaaaattt tttaaagatc cagttgtcta tgttgaccct ggacttatac agcaggtttt
     2461 tctcaatatc ctgctcaatg cagttcatgc catacgtcca aaagggaaag gaaccattac
     2521 cattaccact gatgaatcaa atggtaatgc tataattaaa attcacgata ccggtaccgg
     2581 gatcgatcca aaaatcaaag atgaaatatt caatcctttt ttcactacca aacagggtgg
     2641 agcttataat attgttggca ccggcatggg attatcaatc tcaaaaaaca ttattgagca
     2701 acatagtgga agtatatatt tttacagcat cccggaaaaa gaaaccgaat ttatcattga
     2761 gctccccaaa ttttcttcag cgtatcttcc taatgaaatt aaaatttcat gtgaacccag
     2821 agatatagca aatctttcag tattaattgt tgacgatgat gaagcaatcc gtgatctttt
     2881 taaaacatta ttgcaatcac taggtataac aaccatttct ttcgcaacca acggatatga
     2941 aggatattcc ttatgcaagg attcaaactt tgatattgtt ttcatggatg tatccatgcc
     3001 agttttatca ggaattgatg catataagat gataaaagat gaaaaacctc agcaaaaagt
     3061 tgtttttatt accggtatat tttatgaaga ccagataaaa gagttagttg acaaagaaaa
     3121 cgcctatggc tacattaaaa agccgtttga cataaaagaa ataaaagcat tactatattc
     3181 aatcgcttca tgcaggcgat agttattgaa gaagaggtga aatacaatga aaacaacaat
     3241 tgatgctggc attattggcg gttcaggatt atatcagatt gatggtgtaa cgatagttga
     3301 tgaagtatca gtaaaaactc cgtggggaat gccttctgat ataataacca ttgccgaaat
     3361 tgatggtgtc agggcaggtt ttttaccacg tcatggcaga ggacatttta ttctgcccca
     3421 tgaaataaac taccgggcaa acattgcggc attaaaaatg ctgggagcag ggcaaattat
     3481 tgcattttca gcagttggga gtttaaaaga tcggattaaa cctcttgatt ttgttatccc
     3541 tgatcaaatt attgacagga ctaaatctag aatttccact ttttttgggg atggcattgt
     3601 tgcccatatt gcctttgcag atcctttttg cagcagactg catgaattaa ttttagttgc
     3661 agcacaacag ctcaatatcc ccatgcatac caatgaaata cttatctgca tggaaggacc
     3721 cgctttttca acacgtgcag aaagccatct ttaccgttca tggcaggctg gtgtcatcaa
     3781 tatgagcaca ctacccgaag caaaattagc ccgcgaagca gaaatgtgct acgctgtcat
     3841 ctgcatgagt accgactatg attgctggaa agaagatgaa gaacatgtca cggttgatat
     3901 ggtggtaaac aacctcaata aaaatgctgc aaacgctaag gctttgataa agcatcttgt
     3961 gccattattg aaacaggaaa ggacatgtgc ctgtaaggaa gctatcaaat atgctattat
     4021 gactgattct acaaagcagc cacaaaaaca gaagaaaaaa ttaaaagcta tcctgccaca
     4081 ctatttttca taatgttgtt gatgctgaat gctatcatgg catgagcttt catcagaacg
     4141 ttccatgata gcatatatct cttttgcaag tgttaaaagt gtttgataat tgttaagctc
     4201 gggattatca agtaccttct ccagtagctc attgagtatt ttcccaacca ttggtcccgg
     4261 tttgatatgc aatgcgtcca ttatactata accatcaata tccagatcct taaccgtgaa
     4321 tgcattgtct tttgcaataa ccttttcaat atgtttttta agatatttta ttggttcagg
     4381 caatccatct cgcatgccac taccaactct atccgccaat cgcagtataa acaaatcctc
     4441 cagattgtgc agcccaactt tcctgataaa gcgccttgcc gcaccatcag tccaatcctg
     4501 tgtatagtaa aacatatgat tttctacaag gtgagtaaca tactctatct catcatttga
     4561 aaatttaagc ctgcgcataa tcctgcgggc aatctttgca ccgatgatct catgattgta
     4621 aaaggtataa tcaccgtcat ggccttccct gcgtgttgta atcttgccta tatcatggaa
     4681 taatgcagca agccgtatta aaggcttatc gttaggagca gcatcacatg agtaaatgct
     4741 atggtaatag atatcatata aatgatattt attttgttgc ataccaaatc cttgcgatag
     4801 ttccggtaaa cacaaatcca gcaaaccagt cactcgcata tattcaaaac ctaccgatgg
     4861 cttatctacc tccagcattt tttttatttc atctcgtaca cgctccactg atatctttga
     4921 tgccacatca agcatctgtg ggattgcttc cagtgttttt ttattaatgc tgaaatgcaa
     4981 cttggccgct aatc
//
LOCUS       DAOSOX010000172         4977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17030_length_4977_cov_9.428078, whole genome shotgun sequence.
ACCESSION   DAOSOX010000172 DAOSOX010000000
VERSION     DAOSOX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17030_length_4977_cov_9.428078"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PLX22_10315"
     CDS             complement(<1..1715)
                     /locus_tag="PLX22_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT20332.1"
                     /translation="MIVAKNLSKKFGNSLLFEDVTFSIGSKEKVGLVGRNGLGKTTLL
                     KIIAGTEQPDEGAITIPRHYTLGFMSQNLSFTKMVARDEALSLLPQSEQHEYWRAEKI
                     LSGLGFSNDDMVKRVSELSGGFQVRLQLAKTLILNPDMLILDEPTNYLDITSIRWLAR
                     FLQSWRGELLLVTHDRSFMDAIITHTLGIHRNHIKKIKGTTEQWYEQIAQEEEIYEKT
                     RINDERKQKEIQEFISRFRAKARLANLVQSRIKTIEKMQKREKLIPVKNLDFEFRYKP
                     NNAKYIMQVDSLTFGYNEKPLINNFSLSIASGDRICIIGPNGKGKTTLCELLAGTMKP
                     QSGIITRASGVEIGYFEQTNSIMLNPNATVEDEIVLSDPDKNRQLARNLCGLLLFEGD
                     DALKPISVLSGGEKSRVLIGKILVKPINLLILDEPTNHLDMEACDALLAAIDSFDGAV
                     ILVTHNELLLKALAQRCIIFQKNTITIFEGDYQRFLETIGWENESEPVKKEYENINEQ
                     KLNKKELRRIRSQLLMEKQNECKPLQETVSSIEQEITQHENLLKILNEEIINASQLQK
                     SDDIQ"
     gene            complement(1712..2194)
                     /locus_tag="PLX22_10320"
     CDS             complement(1712..2194)
                     /locus_tag="PLX22_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOT20333.1"
                     /translation="MLLCYILIALCLLIVLKRVVKVVPADSICIIEKKHHYYKTLRPG
                     WHCITPFYSQARIVPVEKRNSIPVTKEITFGDSSRHAVTVSTTFLLWDPYTPFFTFEK
                     LDTSMSNQALKELETIASTCSMQEFTHTIDAIKQKCLDALNQFGKEYGIEFTQIIISL
                     "
     gene            2584..4173
                     /locus_tag="PLX22_10325"
     CDS             2584..4173
                     /locus_tag="PLX22_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT20334.1"
                     /translation="MEPIAEQIKILVLDDDPFIGRMLTSMLIGYNVRCATTFNEFHTI
                     IEEFIPDIAIIDLFLPDANGLDVCKWIRNQSTYEDTILYILTSNTDEQTLVRAYSIGV
                     MDYIIKPFNKFILFSKLHRCALMIEIKRKLETSLEYQKDLKKRLLQLNDFVKKCITID
                     DLDSLVALMVYVQHIVPVDGLCVFAVNNNKYTPLELIPHQRSPKIITALYRAAAITKN
                     DSVLFFFDNKNNIYVSRIALQQHGFVFLVKEKQFNPDEKNMLTLFAELLKVIISRTEI
                     HDYVQKQNRIYQEEISKVRQIQVAQLPKFKNIHGFEIASTFLPAEDISGDFYDGFFIN
                     GNTDIFQIVLCDVSGHGVASSYIGNAIRALIRTYSKENIYPAQVLYKLNQQMAIDGKG
                     LFYYGTIVIVRLYPDGKIVYASGGHPPLYYFNSANQSIEEIQNTGPLVGIFYNIEFKD
                     SILNMNSKDMLLLYTDGIIESMDPELRKLYGFERLKNNIMAFSGEPVMDMLHSICGSM
                     YEFTGYAPLEDDVTLICIKKT"
     gene            complement(4170..4943)
                     /locus_tag="PLX22_10330"
     CDS             complement(4170..4943)
                     /locus_tag="PLX22_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20335.1"
                     /translation="MLTDHEIETLITKLRNKYNDYAHKYNARWFSVDAFEERLQTALR
                     NKINMEAFIVAEIAHFEKIKQRYEQKNTTSFSTKVDALIEELTARINHYPEIAFHPEA
                     HFEIMHMYGACNQLLELYFPVLWLIIDDRSTVYNFEQQLHYLCGRTSEKESKRIEDHI
                     ALLNRHGVKSIEIEKNKNEYLKECAFLLHAIHEWLNNILNNYDTTPLNFSKLYIRGDK
                     KHKIISLFNNETCRSALATIQNYIHQIIEDFRLQAFKKI"
BASE COUNT         1492 a          848 c          938 g         1699 t
ORIGIN      
        1 tgctggatgt catcactttt ttgtaactgt gatgcattga taatttcttc gttaagaatt
       61 tttaaaagat tttcatgctg tgtaatttcc tgctctatcg atgatactgt ttcctgcaat
      121 ggtttgcatt cattttgttt ttccatcaat agttgtgaac ggatacgccg caattctttt
      181 ttattcaatt tttgctcatt gatattttca tattctttct taacaggttc actctcgttt
      241 tcccatccga tagtttctaa aaaacgttga taatcgcctt caaatatggt gatagtgttt
      301 ttttgaaaga tgatacatcg ttgtgccagg gctttgagca gtagttcgtt atgagttacc
      361 agtataacgg caccgtcaaa actatctatt gccgctaaca gcgcatcaca tgcttccata
      421 tcaaggtgat tcgttggttc atccagaatg agcaggttaa taggtttaac cagtattttt
      481 cctataagaa cacgactttt ttcgccacct gaaagaactg agattggttt taatgcgtca
      541 tcaccctcaa aaagaagcaa accacaaagg ttccgggcaa gttgtctatt tttatctgga
      601 tcgcttagca caatttcatc ttcaacagta gcattaggat taagcataat actattggtt
      661 tgttcaaaat agccaatttc aactcctgat gcacgagtaa taatccctga ttgtggtttc
      721 atggtgccag caagaagttc gcaaagggtt gttttcccct taccgttagg gccaatgatg
      781 caaattctat cacctgaagc aatggacaaa gaaaaattat tgattaaagg cttttcgtta
      841 tacccaaagg ttaacgagtc aacctgcatt atatattttg cattattggg tttgtacctg
      901 aattcaaaat ctaaattttt taccgggatg agcttttctc ttttttgcat tttttctata
      961 gttttaattc ttgattgtac aagattagca agacgggctt ttgctctgaa acgtgaaata
     1021 aactcttgaa tttctttttg cttacgttca tcgtttattc tggttttttc ataaatttct
     1081 tcttcctgcg caatttgttc ataccattgt tctgtggtgc ccttgatttt ttttatgtga
     1141 ttacgatgta tacccagtgt atgagtaatt attgcatcca taaaacttct gtcatgggta
     1201 acaagtaaaa gctcaccccg ccatgactgc aaaaaacgcg ctaaccatcg tattgatgtt
     1261 atatctaaat agttggttgg ttcatccagt atgagcatgt cagggtttag tatcaatgtt
     1321 tttgccagtt gtagccttac ctgaaatcca ccggataatt cagaaactct ttttaccata
     1381 tcgtcatttg aaaaacccaa ccctgatagt atcttctcgg ctcgccagta ttcatgttgt
     1441 tcagattggg gaagcaaaga cagtgcttca tcacgtgcta ccatttttgt aaagctaaga
     1501 ttctgggaca tgaagcccag ggtataatgt ctgggaatgg ttatagcgcc ttcatcaggc
     1561 tgttctgttc ctgcaataat ctttaaaagg gttgttttgc caagcccatt acgcccaaca
     1621 agtccaacct tttccttact gccaatagaa aaggttacat cctcaaagag caatgaattc
     1681 ccaaattttt ttgaaaggtt ttttgctact atcatagtga tattattatt tgtgtgaatt
     1741 ctattccgta ctcttttcca aactggttta atgcgtcaag gcatttttgt tttatagcat
     1801 caatggtatg ggtaaattcc tgcattgagc aggtagaagc aattgtttct aattctttga
     1861 gagcctggtt gctcatgctt gtgtcaagct tttcaaaggt gaagaaaggg gtgtacggat
     1921 cccataataa aaatgttgtt gatacagtga ctgcatgtcg ggaactatcg ccaaacgtta
     1981 tttctttagt tactggaatg ctgttgcgtt tttcaacggg tacaatacgt gcctgagaat
     2041 aaaaaggagt aatgcaatgc cagccaggcc gtaacgtttt gtaatagtga tgtttttttt
     2101 ctattataca tattgaatca gcgggtacaa cttttaccac tcgctttaac actatgagca
     2161 aacaaagcgc aataagtatg tagcacaata acataaccta cctccaataa aatatttaaa
     2221 taattataat taagaagctg caatagcaat taaggtcacg caatatcgtt aaccaaaatt
     2281 ttatagtaaa actgaatgct aatagtatac aatgatagta atttttgcaa taatttcaat
     2341 aaaagatttt tagtattttt tggcgatagt catcctgaaa cgcaggacgt aatcctgaat
     2401 tcattgcagg atataacgtt actcatatgg attccggaat gacggcagtg gcaaaagaga
     2461 ttccgggtca agcccggaat gacaggggtg catattgcta tttttggaaa aataccaata
     2521 aaaatacact tgaaaatatg gaaatgtttg tgtataaaag atatatattt gcaggtaaaa
     2581 aatatggaac ccattgctga acaaataaag atacttgtgc tggatgatga tccgtttatt
     2641 ggcaggatgc tgacctctat gcttataggg tataatgtca ggtgtgctac aacttttaat
     2701 gaatttcata ctataattga ggaatttata cctgatattg ccattattga tctttttttg
     2761 ccagatgcca acgggcttga tgtgtgtaaa tggatacgga atcaaagtac gtatgaggat
     2821 acaatactct atatattgac atcaaacact gatgagcaaa cgcttgtacg ggcctatagc
     2881 atcggtgtca tggattatat catcaaacca ttcaataaat ttatattatt ttcaaaattg
     2941 caccggtgcg ctctgatgat agaaattaaa cggaaattgg aaacctcact ggaataccag
     3001 aaggacttaa aaaaacgttt gctccagctt aatgattttg ttaaaaaatg tataactata
     3061 gatgatctgg attcactggt tgctcttatg gtgtacgtcc agcatatcgt tcctgttgat
     3121 gggctatgtg tatttgcagt taataataac aaatatacac cattagaact aataccacat
     3181 caacgttcac caaaaattat tactgcatta tatagggctg ctgctataac taagaatgac
     3241 agcgttttgt ttttctttga taataagaac aacatctatg tttcacgaat tgcactgcag
     3301 cagcatggat ttgttttttt agtaaaggag aagcagttta atcctgatga aaaaaatatg
     3361 ctcacattgt ttgctgaatt gctaaaagta attatcagca gaactgaaat tcatgattat
     3421 gtgcaaaagc aaaacagaat atatcaggag gagatatcca aagtaagaca gatccaggtt
     3481 gcgcagttgc caaaatttaa aaatattcat ggatttgaaa ttgcgtctac atttttacct
     3541 gcagaagata tctcgggaga tttttatgat ggatttttta ttaacggcaa tactgatatt
     3601 tttcagatag tgttatgtga tgtgtcgggg catggggttg cttcatcata tatcggcaat
     3661 gccatacgcg cattaatacg aacttatagt aaagagaata tataccctgc acaggtgctg
     3721 tataaactaa atcaacagat ggcaatagat gggaaagggc tcttctacta tggtaccatt
     3781 gttattgtta gattatatcc tgatgggaag attgtgtatg catcaggggg tcatccgccg
     3841 ctgtactact ttaacagtgc aaaccagagt atagaggaaa ttcagaatac aggcccttta
     3901 gtaggaatat tttataacat agagtttaaa gactctattc tgaacatgaa cagtaaagac
     3961 atgctattgt tatatactga tggaattatt gaatccatgg accctgaatt gagaaaatta
     4021 tatggttttg agcgtttaaa aaataatatt atggcatttt ccggtgaacc tgttatggat
     4081 atgctgcatt ccatttgcgg cagcatgtac gaatttaccg gctatgcacc gctggaggat
     4141 gatgttaccc ttatctgtat taagaaaacc taaatttttt taaatgcctg cagtctaaaa
     4201 tcttctatta tttgatggat atagttttgt attgttgcaa gtgccgagcg gcatgtctcg
     4261 ttgttaaaca aacttattat tttgtgtttt ttgtcgccac gtatataaag ttttgaaaaa
     4321 ttgagaggag tagtgtcata gttattaaga atattgttta accactcatg tatggcatga
     4381 agcaaaaatg cgcactcttt taaatattca tttttatttt tttctatttc aatgcttttt
     4441 acaccatgac ggtttaatag tgcaatatga tcttctatcc gctttgattc tttttcagat
     4501 gtacgtccac aaagatagtg aagctgttgt tcaaaattat acaccgttga cctgtcatca
     4561 atgataagcc ataatactgg aaaatacagc tccagcagtt ggttacacgc accatacatg
     4621 tgcattattt caaaatgggc ttctggatgg aatgctatct ctggataatg gttgatcctg
     4681 gcagtgagct cttcaatgag cgcatcaact tttgtgctga agcttgtagt gtttttttgc
     4741 tcatagcgtt gttttatttt ttcaaagtgt gcaatttcag caacaataaa agcctccatg
     4801 ttgattttat tacgcaatgc ggtttgcaaa cgctcttcaa aggcatcgac ggaaaaccag
     4861 cgggcattgt atttatgtgc gtaatcatta tatttattac gcagttttgt aatcagggtt
     4921 tcaatttcat gatctgtaag cataaaaaat ttatgttcga tagttattgt tcattat
//
LOCUS       DAOSOX010000173         4959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17102_length_4959_cov_5.091558, whole genome shotgun sequence.
ACCESSION   DAOSOX010000173 DAOSOX010000000
VERSION     DAOSOX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17102_length_4959_cov_5.091558"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="PLX22_10335"
     CDS             complement(<1..1595)
                     /locus_tag="PLX22_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20336.1"
                     /translation="MEWVKTKGAFLFISIIMMLLALTPLYGRSWDDLDEEHEVYGSEK
                     EEKYPIKSLFIEQEKWQNHYSMMVLWLYKYTDYPNYTSYRVLPLYYHITSKIDNRKKT
                     IIFPLLTYVKQDGAEKTKFLLFPAYYSYTNQVKYDREILLLLWWGKQNRAQEYQQFTS
                     QYNVIVPLLSYYRVYRSDELSQTWYGNPLFWYSSHSTPHDSEYTWWAPIIPLTYHHVR
                     NNYGHRNIVWLLDYSWDEHGLQRLWCLPVFMWGKDLSKATIEHYTVTPLFGRRTVYDT
                     STQHYTYNNIWFPVIPVYYHSAGDDSSHTNILWLIDWRRNHNALHTLWIVPLLFHEFT
                     DNGYRYYVPLYCSPRRTAYEGVSFGLFHFHRWQPDMDRLWIWLYYHSKHYAINNAPQK
                     NGVEQYYIHFLPIYFSWRNDTSKGNILLPFVYNYSDTNTTVHINLTGYARKTYTGIFK
                     PDVGVNLSQSEGRKYLDTDVSWLYAMWSFSSRIPYSRDKANTGLGDSYLQNADAVDNN
                     KPADYDADSALMKRTIANNHLNG"
     gene            complement(1722..2267)
                     /locus_tag="PLX22_10340"
                     /pseudo
     CDS             complement(1722..2267)
                     /locus_tag="PLX22_10340"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            complement(2552..3664)
                     /locus_tag="PLX22_10345"
     CDS             complement(2552..3664)
                     /locus_tag="PLX22_10345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT20337.1"
                     /translation="MDVFDLVVFFIAFIISLVFIPVIIKVSQKNGLYDYVNERKIHKG
                     NISRLGGLAIFIGFMCSLVYLLVYDFKVQFNIPLFAGAVSLAFLTGFVDDIKKISARY
                     KLFLQLVCGLLVALAGLQINGITVSPELSIQFGVLSYVVTILWVAGFMNAINMLDGMD
                     GLAGGIVMIASLFLAIIGWLQGMVVVTYLAIALMGAIGGFFIFNYPPAKIFMGDGGAY
                     FLGFMYAILPLIGIKKVSTITVFFVPLVLLLIPLIDMAHVVQNRFVGGNHIFLADRSH
                     IHHRLLHIGFSNKGILWILYSYTAILGCFSIVLLYLKPHQSIIITILLFLLTVLAFFT
                     ISVAEGRIAQLEGNTNNKKSNKGLLKHIAKHKFSKA"
     gene            complement(4114..4320)
                     /locus_tag="PLX22_10350"
                     /pseudo
     CDS             complement(4114..4320)
                     /locus_tag="PLX22_10350"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(4336..4665)
                     /locus_tag="PLX22_10355"
     CDS             complement(4336..4665)
                     /locus_tag="PLX22_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20338.1"
                     /translation="MKAGRGFFISSSGWVKTHGKYSIEKELELAWLFGVIDHFNSEVI
                     RFIMVKHGDRFESGRAIQQSILRLYGTSGMNCSMGLEVRCDLGGQYTSAYCKNTMEYY
                     GIQISYS"
     gene            complement(4637..4768)
                     /locus_tag="PLX22_10360"
     CDS             complement(4637..4768)
                     /locus_tag="PLX22_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT20339.1"
                     /translation="MPTCARKKEEIVLRLLQGESIEMLSREVKVSVGDIESWKRLFY"
BASE COUNT         1631 a          977 c          716 g         1635 t
ORIGIN      
        1 ctaccattca aatgattatt ggcgatagtt ctcttcatca aggcactgtc agcatcatag
       61 tctgctggtt tattattatc aacagcatca gcattttgca aataactatc gcccaaacct
      121 gtatttgctt tatcccgtga atatggaata cgagatgaaa agctccacat tgcatataac
      181 cacgatacat cagtatcaag atattttctc ccttcagatt gggaaagatt aaccccaaca
      241 tctggcttaa aaataccagt ataggtttta cgcgcatacc ctgtaaggtt aatatgaaca
      301 gtagtgttgg tatcactata gttgtaaaca aaaggcagta gtatattccc tttgcttgta
      361 tcattacgcc atgaaaaata gatgggcaaa aaatgaatat agtattgctc aacaccgttt
      421 ttttgaggcg cattatttat ggcatagtgc ttgctatggt aatacaacca tatccatagc
      481 ctatccatat ccggctgcca gcggtgaaaa tgaaataagc caaaggaaac accctcataa
      541 gctgtcctgc gtggactgca gtataatggc acatagtagc ggtatccatt atcagtaaat
      601 tcgtgaaaca gcaatggcac aatccacagg gtgtgtaatg cattatggtt tcttctccaa
      661 tcaatcaacc atagtatatt ggtatgtgag ctatcatcac ctgcactatg ataatataca
      721 gggataaccg gaaaccagat gttgttgtag gtatagtgtt gtgtgcttgt gtcataaaca
      781 gtgcgcctgc caaaaagtgg tgttaccgta tagtgttcga tagtagcctt tgataagtct
      841 ttaccccaca taaataccgg caaacaccat aaacgctgca acccgtgttc atcccagcta
      901 taatccagca accatacaat attacggtgt ccatagttgt tgcgcacatg gtggtaagtg
      961 agcggtatta ttggtgccca ccatgtatac tcactatcat gaggagtact atggctacta
     1021 taccaaaaaa gtgggttgcc ataccatgtt tgtgacagtt catccgaacg gtatacacgg
     1081 taatatgaca gcaatggaac tatgacattg tactgagagg taaactgctg gtattcctgt
     1141 gcacgatttt gtttacccca ccacaataac agcaaaatct cacggtcata tttaacctga
     1201 ttggtgtatg aataatatgc aggaaataat aaaaactttg ttttctctgc accatcctgt
     1261 tttacatagg ttaacagggg gaagataatg gttttttttc gattatcaat tttactggta
     1321 atgtgataat ataatggaag tactctgtat gaagtatagt ttggataatc agtatactta
     1381 tatagccata gaaccatcat tgaataatgg ttctgccatt tttcctgttc tataaaaagc
     1441 gattttatag gatacttttc ctctttttca cttccataca cctcatgctc ttcatccaga
     1501 tcatcccagc ttctgccata caatggagtc aaagccaaaa gcatcatgat aatggaaatg
     1561 aaaaggaatg cacctttggt ttttacccat tccatagttt tcttattcac ctgctatgtt
     1621 gtataatttt ccagttttac acttcggatg gaacattata catcaaacat aataaccttg
     1681 aacactttaa atcaaagtac atcatccaaa agtttacaac actacgtaac atccaattcc
     1741 tttatctgtt gctgtatata gtcccgtatt tgatattttg taattgatat aacaggttca
     1801 agatgattaa tcttatcgtc aataatatca tctacatata acaatgccat agaaaatagt
     1861 ttcattgtta atccaggtat tatttttgaa gatatatcat aaagtttttt tattcccaat
     1921 tctttcatta aaaaatatac atcataataa tctctatatt tagctcgtaa aaataataca
     1981 tgtatcttca tggcagcaat ggattcaata gttgcaatat tcagttgttt aatttttctt
     2041 ggttttaaaa attcccagcc agcatcaaaa aatgttactt ttgttccatc aatcaaaaga
     2101 tcaatctgct cactgctttg atttgtaatt tccacctttc caaaatattt tatatactgc
     2161 aaaatgtcat gtatgctata gagattttta ttgtacgtaa aaaaatctaa atcatcgctt
     2221 ttcctatgat gtaaatatat tgctaacaca gagccaccag ctaaaacata gctgttgttt
     2281 tgtttgtttc atagacacat gcactattat tttattatgg ttacttttaa caataaaatt
     2341 ttcatcattt caaaaaatag taaaccattt tctgaatgat tataccttca ggatatacca
     2401 aatataatta tgataacttc ccctatagca aagctgtatt agtatactat gatacattat
     2461 ttttgtcaaa tgtttggtta tatggtatgc tacattacac actagttcaa gctaaggatg
     2521 cactattcat atattgctat tacaatttta actatgcctt tgaaaattta tgttttgcaa
     2581 tatgtttcaa taaaccctta ttgctttttt tattattagt attaccttcc aattgagcaa
     2641 tacgcccttc agcaactgat atggtgaaga aagcaaggac agtcaataaa aataaaagta
     2701 tcgttataat aatagattgg tgaggtttaa gatacagcaa cacaattgaa aaacatccaa
     2761 ggattgccgt gtagctgtat aatatccaca atatcccttt gttactaaaa ccaatatgaa
     2821 gcaatcggtg atgtatatgg cttctgtcag ctaaaaagat atgattcccg cctacaaatc
     2881 tgttttgcac tacatgtgcc atatcaatca gcggtatcaa tagcaacacc agcggtacaa
     2941 aaaatactgt tatggtagat acctttttaa tacctatcag tggtaatatt gcatacataa
     3001 agcctaaaaa gtatgcccct ccatcaccca taaaaatctt cgcaggagga tagttaaaaa
     3061 taaaaaatcc accgatggca cccatcaatg caattgctaa ataagtaacc actaccattc
     3121 cctgtagcca gccaataatt gccaaaaaca atgaagctat cattacaatg ccaccggcaa
     3181 gcccatccat accatcaagc atattgatgg cattcataaa tccagcaacc cataatatgg
     3241 taacaacata tgatagtacg ccgaattgta tgctcaattc aggagataca gtaatcccgt
     3301 taatctgcaa tccggctaag gcaacaagca aaccacatac taactgtaaa aataacttat
     3361 agcgtgcaga aatctttttg atatcatcaa caaaacctgt taaaaatgcc agcgatacag
     3421 caccagcaaa cagtggtata ttgaattgta ctttaaaatc atatactaaa aggtatacca
     3481 gcgagcacat aaacccaata aagatagcca gcccgcccaa tctgctgatg ttgcctttat
     3541 gaattttgcg ttcattaaca taatcataaa ggccattttt ttgactaact tttataatta
     3601 caggaataaa tactaatgaa ataataaatg ctataaaaaa aacaaccaaa tcaaatacat
     3661 ccatgacagt gcaccttttt ttgtttatga ttacaaacct atactgttct tattttcaat
     3721 agtgcaacag tttttgtatt agtcaaggat tttttatgca tgttttgtta tgtgctatgt
     3781 aggtattaat tattttgttt gcctataatt aggttaacat cagcatctcc gtcatacacc
     3841 ttattacata ctatatcagt ataatgatat actataaaac tattaatact gaacttgctc
     3901 atgcaacact aaattccgat gctcagtata atgatatact ataaaactat taatacccta
     3961 gacatggaat tttatattaa cttattgttt atgggcgtat cgtttataaa atagtattat
     4021 ttgatataat agaaccaata aaaataaagt actgtacaaa aacaatacta aacgtatttc
     4081 atgtaacaat aatgaaagtg ttctggcccc gatttaacaa gcacttttta tgctcgtatc
     4141 ataatattcc tgtaatgcct catgcggact tttatatcct aatcgttcta tcatccactc
     4201 ttcgttataa cgcttaataa attcttgtac ggcatctatt gcttctgcaa tggtttcaaa
     4261 atcatttacc gaaaacacct gctcttctaa tgttctaaaa aatcgctcta ttatcccatt
     4321 acattctgga cttcgtcacg aataacttat ctgtatccca taatattcca tcgtgttctt
     4381 acaatacgcc gatgtgtatt gcccccctaa atcacatcgc acctcaagac ccatggaaca
     4441 gttcattcca gaggtcccgt atagcctcag tatgctttgt tgtattgccc tgcctgattc
     4501 aaatctatcc ccatgcttca ccataataaa ccttattact tcagaattaa aatgatcaat
     4561 aacgccaaat aaccaagcta attctaactc cttctcaata gaatatttcc catgcgtttt
     4621 aacccatccc gagctgctaa taaaaaagcc tcttccagct ttcaatatcg cctactgata
     4681 cttttacctc ccgtgaaagc atttctatac tttcaccctg taataaccgt aatactattt
     4741 cttctttttt ccttgcacag gtgggcactt atttatttta tggtttcatc tgtactcata
     4801 tctattccac tttttcaatc taaattgtat actattttgt gctcggttat aattggggcc
     4861 aatataaaaa atattccttg ttaactagtt tattcataaa gaacaaatcc attcaactaa
     4921 aattgttgca ttacgtttat tattatgatt atcttctac
//
LOCUS       DAOSOX010000174         4957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17111_length_4957_cov_8.716646, whole genome shotgun sequence.
ACCESSION   DAOSOX010000174 DAOSOX010000000
VERSION     DAOSOX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17111_length_4957_cov_8.716646"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..777
                     /locus_tag="PLX22_10365"
     CDS             292..777
                     /locus_tag="PLX22_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20340.1"
                     /translation="MDFETTTCISYEHLNTLYSLADKFNMPLHSCIVYLLIYAAKKEK
                     APAKAFKGISYRSRNKLNPWKRVHLYLAFKEYEYLLDVKKIWKMSVARAIAFCVENVL
                     DEFVQFLNNLIEAEREGDIDNYLVYEINRSYLFEYDTKEGVHCCRFYWGLPRKYARCK
                     P"
     gene            1036..1638
                     /locus_tag="PLX22_10370"
     CDS             1036..1638
                     /locus_tag="PLX22_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20341.1"
                     /translation="MVKKLSLCIMALCMACMTTGLLAQELTDDVAFLRVGYQWNTVNF
                     DKNLPVIGDNLENNGIAIEGEYNLNLGGWLLGVSLEWARLVNDDDSDFVFYYLSPMIS
                     LKFIAAGGLYIGPGISARYMMSADTPSGVDEPDKEVDLWVNAVAGWMTPIAEAVYLDV
                     QARFGWNLTKNQFEDVGDIDNNYDYAVYVGIGTRTRSTGI"
     gene            complement(1712..3769)
                     /locus_tag="PLX22_10375"
     CDS             complement(1712..3769)
                     /locus_tag="PLX22_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT20342.1"
                     /translation="MNNTTLVSVTIAMIFSCSSLAYSAEATYDMRNLTFDTTTTYAIK
                     AQWLFWWNQIIDPVSIDDNKAPQPTDIVMMPQHWTDYKLNGTLLPAKGCATYALKILL
                     PDNAIYGIYLDHMVTAYRLYINGVEVANNGKVSKNLSAMRNECRHQAIYFTPKNKTAL
                     CVLQISNRDYRTAGMWHPLIVGKDRAILAHYNTKVISDLFLFGAILIIGLYNIALFLF
                     RTRDRAPLWFGLFCLTVCARIAATGSTTIANIFPGFPWELQIKMELSVFYLGSLFFGL
                     FFHEIYPMEIKKKHLQFFNGIFILLTVLAITTPVSFFNRLVPYMQIAVMAGLSYILVC
                     LIIAALRKRPHIHYFLAGFVLFMVTAVNDILYAKNILPTMYILPAGLFVFIFTQAIAI
                     ARIFSFSFLQVEHLSAHLSDLNISLERFVPHEFLSFLQKQSILDVQLGDQTLKDITIL
                     FADIRSFTTLSEKMSPEETFKFLNSYLNRIGPIIRSHNGFIDKYIGDGIMALFPQSPQ
                     DALNAAIAMVKRIAEYNDERKKYQLSPISIGIGIHTGSAILGIIGETMRIESTVISDT
                     VNLSSRIESLTKHFRTTILVSGDVYEQVKDIPEYNFRYISKVIVKGKSQPTKVYEVLS
                     MYPDDAVALFTSTKEQFKKALTLYNKKQYTEALHIFKEIALKNPCDMITKNFIEDMEE
                     KKD"
     gene            complement(3952..4944)
                     /locus_tag="PLX22_10380"
     CDS             complement(3952..4944)
                     /locus_tag="PLX22_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT20343.1"
                     /translation="MDNTEKPLITISFNLNAEEKDRIESVLSPIARIIYIKDLNADER
                     AKLLQTSHVVLAWAPHSEFSPAEITLLEKVKFMQLLSAGADHLDFSRLPANLTVASNV
                     GAYAQPMAEHVLAMVLALSKRLYIHHEKLKQGIFDQFSENRALSGTTAAILGFGGIGR
                     EVARLFKPFNMHILAINRSGKSPDPAEFLGTPDDMGKVLPLADIIVITLPLNRATRGL
                     IGEREFKLMKPDSILVNVGRGEIIDEGAFYHHLKNTPTFMAGIDAWWTEPFRHGHFEM
                     KYPFLDLPNVLGTPHNSALVHGALATAAQKAAQNIRRFLLSEAITGLVRPEDYE"
BASE COUNT         1518 a          882 c         1046 g         1511 t
ORIGIN      
        1 gcacggcggt tagaaaataa tgtacagtat gtaaaaccaa ccccggatga acatgctcgc
       61 ttactttcac agatacatga caataaagag ccgctgtata caaacaatgc atttatacag
      121 aaaaacatgc agtatacaat tcccggtact ttttttgatg caattgcata aataatcaat
      181 acaccaataa aatatatagt attaaaatat agttgaataa tatgatgtta ttttatagtc
      241 taccattata aatagaaaaa taatagtata tagtatatag gagattgtac catggatttt
      301 gaaaccacta cttgtatttc gtatgaacat ttaaacacac tttatagttt agcagataaa
      361 tttaatatgc ctcttcactc atgtattgta taccttctta tctatgccgc aaaaaaagaa
      421 aaagcaccag caaaagcgtt taaaggtatt tcatatagaa gcaggaacaa gcttaatccg
      481 tggaagaggg tgcatttata tttagcattt aaggagtatg agtatttact ggatgttaag
      541 aaaatatgga aaatgagtgt tgcaagggca attgcgtttt gtgttgaaaa tgtgctggat
      601 gagtttgtac aatttttaaa taacttgatt gaagcagaaa gggaagggga tatcgataac
      661 tatctggttt acgagataaa tagaagctac ctatttgaat atgatactaa agaaggtgtt
      721 cattgctgcc gattttattg gggattgcca cgaaaatatg cccgatgtaa gccatagatt
      781 aaaaaaccaa ttttttaatc aacaatgacc attcatcaaa agcatgtcaa ataggctatt
      841 tactaaaata acattaatac catgagcata aataagtaag gccaatgtat cggtaaaaaa
      901 aggtgtatga ataaaaataa ggcttgattt tcatatatac taattgctat aatgcgaaat
      961 ctctattatt acattttgtt ttatgataca taaaacaaag atgagtaaag attatattat
     1021 ttcaggaggt tttcaatggt taaaaaattg tcgttgtgta tcatggcttt atgcatggca
     1081 tgtatgacta ctggattact ggcacaggaa ctaactgatg atgttgcttt tttgcgtgta
     1141 ggctatcagt ggaatacagt gaattttgat aagaatttac cggtcatagg tgataatttg
     1201 gaaaataatg gtatagcaat agaaggtgag tataacctga acttaggtgg atggttgctg
     1261 ggagtttcac tggaatgggc acgtttggta aatgatgatg atagcgactt tgttttttat
     1321 tacctttcgc caatgatttc attaaaattt atagctgccg gcggcttata cattggtcct
     1381 ggaatttctg cccggtacat gatgagtgca gatacacctt caggtgttga tgaacctgac
     1441 aaagaggttg acctgtgggt caacgctgtt gcaggctgga tgacccccat cgccgaggct
     1501 gtttatctgg atgttcaggc acgatttggc tggaacctta ccaaaaacca gtttgaagat
     1561 gttggtgata ttgataacaa ctatgattat gctgtgtatg ttggtatagg tacacgcacc
     1621 cgttcaacgg ggatatagta tagacatgca tttttatagc taacagctta gaaaacagta
     1681 caggcaggga aaccctgcct gtactgtttt cctagtcctt tttttcttcc atgtcctcta
     1741 taaaattttt tgtaatcatg tcgcaggggt tttttaatgc aatctcttta aagatgtgaa
     1801 gcgcttcagt atactgtttt ttgttataga gggttaaagc cttcttgaat tgctcctttg
     1861 tagatgtaaa cagcgccact gcatcatccg gatacatgga taatacctca taaacttttg
     1921 ttggttggga ttttcccttt actattacct tactgatgta gcggaagtta tattctggaa
     1981 tgtcttttac ctgttcgtag acgtcacctg aaacaagtat ggtggtgcga aaatgttttg
     2041 ttaagctctc tatacggctt gataggttta ctgtgtcgga tataacggta ctttcaatac
     2101 gcatggtttc gccaattatt cctaagatag cgctgccggt gtgaatacct attccaatac
     2161 tgatgggtga aagctgatat tttttacgct cgtcattata ttcagcaata cgttttacca
     2221 tggctatcgc cgcattcaat gcgtcttgag gagattgcgg gaaaagcgcc atgatgccat
     2281 cccctatgta tttatcaata aaaccgttat ggctgcgtat aattggcccg atacggttaa
     2341 ggtacgaatt taaaaatttg aatgtttctt ccggagacat tttttcggat aacgtggtaa
     2401 acgaacgaat atctgcaaag aggatggtaa tgtctttgag cgtctggtcg ccaagctgca
     2461 catcaaggat gctctgtttt tgcaaaaatg ataaaaattc atgcggaaca aagcgttcaa
     2521 gcgatatgtt gagatcgcta agatgtgcgg aaagatgctc aacctgcaaa aaagaaaatg
     2581 aaaagatgcg tgcaatagca atagcctggg taaatataaa aacaaacaat cctgcaggaa
     2641 gaatatacat ggttggcaat atattttttg catagaggat gtcattaacg gctgttacca
     2701 taaatagcac aaaccctgca aggaaatagt gtatatgcgg acgtttgcgt aatgctgcaa
     2761 tgataagaca aacaagaata tatgaaagtc ctgccataac tgctatttgc atataaggta
     2821 ccaacctatt aaaaaatgaa accggtgtgg ttattgccag cactgtcagc agtataaaaa
     2881 tgccgttgaa gaattgcaga tgtttctttt ttatttccat gggataaatt tcatggaaaa
     2941 aaagtccaaa gaaaagactg cctaaataaa atacactcaa ttccattttg atctgcaact
     3001 cccatgggaa tcccggaaat atattggcaa tggttgtact gccggtagct gcaatgcggg
     3061 cacatactgt taaacaaaat aacccaaacc acagtggagc cctgtccctg gttcggaaaa
     3121 gaaacaatgc tatgttgtat aaaccaatga tgaggattgc gccaaacaga aaaagatcgc
     3181 ttatgacttt agtgttgtag tgcgcaagta ttgctctatc tttaccaaca atgagggggt
     3241 gccacatacc agcggtgcga tagtcccggt tactgatttg cagcacgcac aatgcagttt
     3301 tattttttgg agtaaaatat attgcctggt gcctgcattc gttacgcatt gctgacagat
     3361 tctttgacac cttcccgtta tttgcaacct caacaccgtt gatgtacaat cggtacgctg
     3421 tgaccatgtg gtctaaataa atcccataga tggcattatc aggaaggagt atcttcagtg
     3481 cataggttgc acatcctttt gcagggagca aggtgccatt gagcttgtag tcggtccagt
     3541 gttgcggcat cataacgata tcggtaggtt gaggcgcttt gttgtcatca atagatacag
     3601 ggtcaatgat ctgattccac cagaatagcc attgtgcttt aatggcatag gtggttgtag
     3661 tatcaaatgt tagattacgc atgtcatagg tagcctcagc gctataagcc agagaagaac
     3721 aagaaaaaat catggcgata gttactgata caagagtggt attattcata atggattatc
     3781 ctgtaataca atatttatat gatattacag tataatcaat actattgcaa tagaaaaatg
     3841 tttttggtat aaaggcagct gtggttgatg cagactacac aaggttgatt tttaatcctg
     3901 aaatgtgatt gcattacttt ataccagttt atatttccct gtcaagactg cctattcata
     3961 gtcttcaggg cgtacaagcc ccgtaatggc ttctgatagc aaaaatcgcc tgatgttttg
     4021 cgcagctttt tgtgctgctg tagcaagtgc accatgcaca agtgctgaat tatgaggtgt
     4081 gccaagcaca tttggcagat caaggaaggg atacttcatc tcaaaatgac cgtgtcgaaa
     4141 cggttcagtc caccatgcat caatcccggc cataaaggtt ggagtatttt taaggtgatg
     4201 gtaaaatgct ccttcgtcga taatttcccc gcggcctacg ttaaccaaaa ttgaatctgg
     4261 tttcatgagt ttaaattcac gctcgccaat aagccctcgc gttgcacggt ttaagggcaa
     4321 tgtgatgaca atgatgtcgg caagtggtaa taccttgccc atatcatcag gtgtacctaa
     4381 aaattcagcc ggatcaggac ttttgccact acggttaatg gcaaggatat gcatgttaaa
     4441 aggcttgaaa agccttgcca cctctctacc aatcccgcca aaacccagta tagctgcggt
     4501 ggtgccggac aatgctctgt tttccgaaaa ttggtcaaaa attccttgct ttaacttttc
     4561 atgatggata tagagtctct tgctcaaagc cagcaccata gcaagaacgt gttcagccat
     4621 tggttgggcg taggctccta cattgcttgc cactgtcagg tttgctggca gacggctgaa
     4681 gtcaagatga tcagcgccag cagacaaaag ttgcataaac tttacttttt ccagcaaggt
     4741 aatttcagct ggtgaaaatt cactgtgtgg tgcccatgcc agcacaacat gggatgtttg
     4801 caacaatttt gcgcgttcat cagcgttgag gtcttttata taaataatcc ttgcaatggg
     4861 agacagaaca gactctatac gatctttttc ttcagcattt aagttaaagc ttattgttat
     4921 aagaggtttt tcagtattgt ccatatcatt cctcctt
//
LOCUS       DAOSOX010000175         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17144_length_4951_cov_7.249183, whole genome shotgun sequence.
ACCESSION   DAOSOX010000175 DAOSOX010000000
VERSION     DAOSOX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17144_length_4951_cov_7.249183"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLX22_10385"
     CDS             complement(<1..621)
                     /locus_tag="PLX22_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20344.1"
                     /translation="MKKFIYLLLTAIMLCTVACFDVIHYIEPRNDKTVFIQFRVITGM
                     VNNKELDDTFTKNNITKLYQGVNFAFTQISTDFKKGFELTATIKENQLKYYKDSATDN
                     NLNTIIPYKDNKNQYILIYDNNEFQANKKDEELSSGLTDAVFSSINYKIIVAGSLKPK
                     HVHLITYNESPKTMYLNPYKVGQQYFIEVPASLILQNLCAIIISSNN"
     gene            complement(802..1701)
                     /locus_tag="PLX22_10390"
     CDS             complement(802..1701)
                     /locus_tag="PLX22_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT20345.1"
                     /translation="MQCWLNGRLCNIHDAHVSLLSHSFGRGTAIFEVLEIVTNNRGTA
                     LLAIHQHLERFTNSAHAMYLNLPYSNEEIVAGIMSCTKANNVTNGLVKFFAYYDDIEL
                     SIIPHNTSISCAIFCMDYEKLPFTREQLLKPARACISSYKKLDAATVPVHAKIAGNYV
                     NGYLAQMEATRKGFTDAILVDTGGNIAEAATSNIFFVMGDSIHTPTLQSIMPGITRMI
                     ILDMLRYEKFNYEECDIHVNDMKHYTEAFFSGSIDNILPIASIDDIAFTPAPGTMTAQ
                     IQQLLTALLNDEHALSQKYLYYF"
     gene            2056..2457
                     /locus_tag="PLX22_10395"
     CDS             2056..2457
                     /locus_tag="PLX22_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20346.1"
                     /translation="MRKKLLNIVYLLGILNNTINKAKAAMKYHQLSLKLIQIRIAEMQ
                     AKYLCMTPFYKKPSLPRDIVVRLTERCFLHCAFCAQGKEGGRVDRKNLKEPPIQFDTL
                     KKIIDETARWKIKPFYKFTGGEPLTMGTPCA"
     gene            3042..3221
                     /locus_tag="PLX22_10400"
     CDS             3042..3221
                     /locus_tag="PLX22_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20347.1"
                     /translation="MLQYNVKNEYELIAKHCPRCQALVMAAVVKTLKGEVLKYKDIRC
                     SNCDWQSPLTQLKSC"
     gene            3359..3865
                     /gene="ndk"
                     /locus_tag="PLX22_10405"
     CDS             3359..3865
                     /gene="ndk"
                     /locus_tag="PLX22_10405"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT20348.1"
                     /translation="MAVEQTLIIIKPDGLVKSLTGNILTRLSEAKLKIVGAKALKVSR
                     ELAEKHYEHLKGKPFFEELIQYIMGELHKTNRVVAFVYEGDNAIQKVRDIVGDTNPEN
                     ANPVTIRGAYGRITTSGVFENVVHASSSPEDAEKEIKLWFEPDELVTTIYPVKKITLE
                     KVSKLVWE"
     gene            3870..4517
                     /locus_tag="PLX22_10410"
     CDS             3870..4517
                     /locus_tag="PLX22_10410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOT20349.1"
                     /translation="MLIIMLGAPGAGKGTIARMLVEKFGIVQISTGDMLRQAVNEGTP
                     VGKKALEFMNSGKLVPDDVIMAIIEERIAQPDCAQGFIFDGFPRTIPQAHALESLLTK
                     KNMTVDAIVNLEVPEEVVLKRLTSRRTCSNPHCQAIYNIYTMPPKKEGICDKCGSSLI
                     QRDDETEEAIKVRLDTYYEKTQPLIDYYCKHKAYFAVSSLDAHETFDAIVQRLQK"
     gene            4624..4710
                     /locus_tag="PLX22_10415"
     tRNA            4624..4710
                     /locus_tag="PLX22_10415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4658..4660,aa:Ser,seq:cga)
BASE COUNT         1646 a          811 c          947 g         1547 t
ORIGIN      
        1 attattagac gatataatga ttgcgcatag attttgcaat atcaatgatg ccggtacttc
       61 aataaaatat tgctgaccca ccttgtaagg attaagatac atagtttttg gcgattcgtt
      121 gtacgttata aggtgcacat gttttggttt gagcgagccg gctacaataa ttttgtaatt
      181 gatagaagaa aaaacagcat cggttaaacc agaagacaat tcttcatctt ttttatttgc
      241 ttgaaattca ttattgtcat atatcaaaat atattgattt ttattatcct tatacgggat
      301 gattgtatta agattattat ctgttgcaga gtctttataa tatttcaatt gattttcttt
      361 gatcgttgca gttaattcaa atcccttttt aaaatctgtt gaaatctgcg taaaggcaaa
      421 attgacaccc tgataaagtt ttgtgatatt atttttagta aaagtatcat ccaattcttt
      481 attgtttacc atgccggtta ttactctgaa ttgaataaat actgttttgt cgttacgagg
      541 ctcaatgtaa tgtatcacat caaagcatgc aacggtgcac agcattatgg cagttaaaag
      601 caaataaata aattttttca tatattcacc agtttttata aaattgtaac gcttgtagat
      661 acaatgaagc agtataattt ttaataacac taacctgact gcatgtcaac aaattatatt
      721 cttcttaaga aataagcctt tcatggacaa cgcaaaaaaa ggtgcattgg ttatattggc
      781 accatgctgg atgaggcagt tttaaaaata atacaaatat ttttgtgata gggcatgctc
      841 atcattcagc agtgcggtca gcagttgttg aatctgtgct gtcatggtgc ctggtgcagg
      901 tgtaaacgca atatcatcaa tcgatgcaat aggcagtatg ttgtcaatgc ttcccgaaaa
      961 gaatgcttcg gtataatgtt tcatatcatt tacatggata tcgcattcct cataattaaa
     1021 cttttcatat cgcagcatgt caagaataat catccgcgta atccccggca ttatggactg
     1081 cagggtaggt gtatggatac tatcgcccat aacaaaaaaa atattagacg ttgcagcttc
     1141 agcaatgttg ccgccggtat caactaaaat agcatcagta aatccctttc tggttgcttc
     1201 catctgagca aggtaaccgt tgacataatt gcccgcaatc tttgcatgca ccggtactgt
     1261 agcagcatct aattttttat acgagcttat acatgcacgg gcaggtttta aaagttgttc
     1321 cctggtgaac ggcagctttt cataatccat acaaaagatg gcgcatgaaa tgctggtgtt
     1381 gtgcggtata atagaaagtt ctatgtcatc ataataggca aaaaatttca caagcccgtt
     1441 tgtcacatta tttgcctttg tacagctcat aattcctgca acaatttctt cattggaata
     1501 gggaaggttc aggtacatag catgcgcact gtttgtaaag cgttctaaat gctggtgtat
     1561 ggcaagcagt gcagtgcccc tgttattagt aactatctcc agaacctcaa atattgctgt
     1621 accccggcca aagctgtgcg agagcaatga aacatgcgcg tcatgtatat tacataatct
     1681 accattaagc cagcattgca ttttggtacc atcctttact gcacatcaca actatgtaca
     1741 accatgaaaa gtattaccta tattcaaaaa ataattttac tatgtgcgat agttattgtt
     1801 tatgtaaaca ttttttctta atagctggca aaaattgtta atatactatc agaatacctg
     1861 ctttcactga ctgagtttta acaattacgt ataatatttt tttcattatt gcatttgcta
     1921 ttttgaaaaa gtgtagaaaa tgagattgag tgtgtgcaat actgttttca tacttttaac
     1981 atttcatcaa ttgtaataag gatgtgtaca atatgaacaa taaagaaaat aactctttat
     2041 caaataatgc taaacatgag aaaaaaattg ttaaacatag tatatttgtt aggaatatta
     2101 aacaacacta tcaataaagc aaaagctgca atgaaatatc accagttgtc actgaaactt
     2161 atccagataa ggattgcaga gatgcaagct aaatatttat gcatgacacc tttttacaaa
     2221 aagccttctt tacccaggga tattgtggta cgcctgaccg aaagatgttt tttacattgt
     2281 gctttttgtg cacaggggaa agagggtggt agggtcgatc gcaaaaattt aaaagaacca
     2341 ccaatacaat ttgacacctt gaaaaaaatt attgatgaaa ctgccaggtg gaaaataaaa
     2401 cctttttata aatttacagg tggtgaacca cttaccatgg gtactccttg tgcttaaaat
     2461 atgattatac tctattaacc atagtattaa aatgctatgg tgatgctgca ctattacact
     2521 attataagta aatcggttga agtactctat agaagcattg ctcaccacca attttcctga
     2581 atactttgtt gtactataaa atcaaaatat gcatcaatgc attacaaata ttacaacatg
     2641 acaattggtt gatatagctt ttgaaattga ataatagtaa tgtcaagcaa taattttttc
     2701 cataaataaa ttgataaagt ttttaatact ttttcgatga gggatacaaa aaaatttttt
     2761 tcagtattta taaagaatga aaaaaatctt gacaaaaaaa tgattatcat taatatgagt
     2821 gactactcac tcatattaat gaaagggata tttttgccga tagtttttat gagtaactat
     2881 cgaacaatat aaaagaagga gggtaaacat tatctttgag aatatactat atatcgataa
     2941 cgatataatg attgacggat gtaaaaaagc atgctataat gcttaaaaaa tacacatata
     3001 ttaataaggg acttttgtta tgattaaacg gaggcgtatc catgttacag tacaatgtaa
     3061 agaatgagta cgagcttatt gccaaacatt gcccacgatg tcaggcgctt gttatggcag
     3121 cggttgtaaa aacacttaaa ggtgaagttt taaagtataa ggacatccgc tgttcaaatt
     3181 gtgattggca aagcccgctc actcagctaa aaagttgttg aaaaaaatac agtgattaga
     3241 gtaatagcac tgccatggtg ataacatcgt attgtgtcga tgtattgaca taattttgca
     3301 tatggatcat ggacacaaat gatgtaaacc ctgtaaaaat actattagag gtaaacctat
     3361 ggcagtggaa cagacgttga ttataattaa acctgatggt cttgttaagt cattgacagg
     3421 aaatatctta acccgccttt ctgaagcaaa attaaagata gttggtgcca aagccttgaa
     3481 ggttagcaga gagttagctg aaaaacacta tgagcattta aaaggaaaac ctttttttga
     3541 agagttaata cagtacatca tgggcgagtt gcataaaacc aatcgtgttg ttgcatttgt
     3601 gtatgaaggt gacaatgcaa ttcagaaggt aagggatatc gttggtgata ccaatcccga
     3661 aaatgcaaat cccgtaacga tacggggtgc gtatggacga attacaacaa gcggtgtatt
     3721 tgaaaacgta gtgcatgctt cttccagccc ggaggatgct gaaaaggaga taaaattatg
     3781 gtttgaaccg gatgagcttg ttactaccat atatccggta aaaaaaataa cattagaaaa
     3841 agtttctaaa ttagtatggg agtaatgata tgctgatcat tatgttagga gcaccaggcg
     3901 caggcaaggg gactatcgcc aggatgcttg ttgaaaaatt tggtattgtg cagatttcta
     3961 cgggtgatat gttacgtcag gcagtgaatg aagggacacc ggttggcaaa aaggctctgg
     4021 aatttatgaa ctctggaaaa cttgttcctg atgatgtcat tatggctatt attgaagagc
     4081 gcattgcaca acccgactgt gcacagggat ttatctttga tggctttccg cgtaccattc
     4141 cgcaggcaca tgcacttgaa tcactgctta caaaaaaaaa tatgacagtg gatgccatcg
     4201 ttaatctgga agtgcccgaa gaagtggtgc tgaagcggtt aaccagccgg cgcacctgct
     4261 caaaccccca ttgtcaggca atctataata tctataccat gccgccaaaa aaagaaggta
     4321 tttgcgataa atgcggcagt agcttaatac aacgtgatga tgaaactgaa gaggctatta
     4381 aggtgcgcct tgatacctac tatgaaaaga cacagccatt gattgattac tactgtaaac
     4441 ataaagcata ttttgcagtt tcctcactgg acgctcatga aacatttgat gcaattgttc
     4501 aacgtttgca aaagtaacaa aaagttcttt acaaacatac aaatcatttt tgatataact
     4561 attccatgat gtatggttga gacgctttat aggtgcattt ctgtactatt tggtagtata
     4621 catggagaga tgcctgagag gtcgaaaggg gcggtctcga aaaccgttga gggcttatgc
     4681 cttccgaggg ttcgaatccc tctctctccg aaactataat agtatctggt ataattaaat
     4741 gtattgacat catcctgcaa agcacctata ttttaagcga tagttgttaa aggaagagca
     4801 gggtgatagt taatggcaag gtatactgtt gcatcaatga taatcttttc tgtattattg
     4861 tcatcttcat atattgctgc taaagatatt ccttatacat tagatgatag agacaggctt
     4921 atcaggcttg aagaaggttt aaaagaaggt t
//
LOCUS       DAOSOX010000176         4936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17211_length_4936_cov_9.083794, whole genome shotgun sequence.
ACCESSION   DAOSOX010000176 DAOSOX010000000
VERSION     DAOSOX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17211_length_4936_cov_9.083794"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLX22_10420"
     CDS             complement(<1..641)
                     /locus_tag="PLX22_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT20350.1"
                     /translation="MNTVIRKGYTYATWLFIGVDTAFWGTISLFSWFVSPRGDLAHFC
                     MQNWSKNILWFCGIRYQVEGIENISKEHVQIFASNHQSFFDIWLLSAIIPVKFGWTAK
                     KELFKIPFMGWHMSLSGYIAIDRKNKERSRASMKEAAVKIRKGNRIMIFPEGTRSRTG
                     VLQPFKKGLFYLCIETGVPIVPIYIHGTYQRLKPETGDINPGKVYVMIGEEMPT"
     gene            complement(737..1882)
                     /locus_tag="PLX22_10425"
     CDS             complement(737..1882)
                     /locus_tag="PLX22_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOT20351.1"
                     /translation="MERSIQFTQEHALFRQTVRKFFEQQVMPFYDTWEHEGIVPREMW
                     NRCGQLGLLLPWLEEQYGGPGGDFLYSVILNEELAYSGANSFMIPLHNDIVAPYIYSF
                     GTEEQKMKYLPKCASGEIVLAIAMTEPEAGSDLAGMKTSAVSNGDSWVLNGQKTFISN
                     GILADLVIVAARSSKELPPHQSVSLFLVEDGTEGFTRGKPIKKIGLKAQDTAELFFDN
                     CRIPDNNLLGQEGQGFIYLMQKLQQERLVCALTSQAAMEKCLAITIDYVKERKLFGKP
                     LSKFQNTQFTIAEMASEIAAGRSFIDDLIHHHIQGHDVVKETCMAKYWICETAKRVAD
                     RCLQLFGGYGYCLEYPVSRFYVDARIQTIYAGTSEVMKMIVSRMMGL"
     gene            complement(1887..4010)
                     /locus_tag="PLX22_10430"
     CDS             complement(1887..4010)
                     /locus_tag="PLX22_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOT20352.1"
                     /translation="MNAFTYCKPEVLNDVLVVTIDCPGKVNKVSSAMLNEMEVIINDV
                     KSKKYKGLAIVSGKDDNFVVGADLDELTSMKTEDEIRAYITKANSVLSGLEKLPFPTV
                     CVINGNCLGGGVELALVTDYRIATDSTNTVIGFPEIKLGLIPAAGGTQRLPRLIGIQH
                     ALPLIVQGSTIRARRAKRLGIIDEIVMPYGLKEIAVKKVLQLKGKRTIKRKRTFAEWL
                     LESNPLGRSIIFSKARTMVMRQTRGLYPAAVAVIDAVEYGYKTTIAKGIAKDSERFIH
                     LVQSYQSQSLVNLFFAMTDLKKNPFKKMEKNVSKLAVIGTGLMGSGIAFVSLPLCDTV
                     LLKDTSLDFVTRSMAQIRKSLEKRAQSGAIRNFDKEVMYGKLIPCDDYTYFKNTDLVI
                     EAVFEDLQLKQNLVKQVEAVCGDTTIVASNTSAIPISLIAKASKRPQNMIGMHYFSPV
                     PAMPLLEIIKTAKTADWVVATAVATGIAQGKSCIVVKDGPGFYTTRILAPYLNESVVL
                     IEEGVPMNDIDSAMLAFGYPIGPIALLDEVGIDVGAHVTIELGQMFTARGAAPSNVLP
                     LLLSKGFKGKKNKKGFYMYDSPKKKGMRLPNKEVYTVIGKVTPEAISVEEIQQRVSLM
                     MINEAAHCLQEGIISSPRDGDVGSVLGLGFPPFKGGIFRYIDHEGAGTIVDTMLQYYN
                     RGLERFKPAMILADMAKTDKRFYKD"
     gene            complement(4022..>4936)
                     /locus_tag="PLX22_10435"
     CDS             complement(4022..>4936)
                     /locus_tag="PLX22_10435"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOT20353.1"
                     /translation="ISKNYRQFIMDLTMFKRPRTIVGKLKLLKGMSFKDFIVPEQPEI
                     GEFSTQLRMGDTADRLAKRLGISQREQDAFAALSHNRAAAAWEQGILKKEVVPVVTPQ
                     GRLVDKDNGFRKGTRAEKLAALKPAFDKRYGTVTAGNSSFLTDGGAAVLLMSETLAKK
                     MRLKPLAYIKSYAFTAQDLWEELLLGPVFSIPKALAMGKLKFKDIGVWEIHEAFGVQM
                     VAVVQCLASDSFCKNRLGLSGKVGEINHDDLNIYGGSLSLGHPFGATGARLVTTCANR
                     LQESGKRYGVVAGCAAGAIGNAIILERS"
BASE COUNT         1372 a         1202 c          888 g         1474 t
ORIGIN      
        1 gttggcatct cctcccctat cataacatat acttttccag ggttaatatc cccggtttcg
       61 ggctttaatc gttgataggt gccatggata taaataggaa ctatcggcac gcctgtctca
      121 atgcataaat aaaataaccc ttttttaaag ggttggagca ctcccgtacg gctgcgcgta
      181 ccctcaggaa atatcataat acggtttccc tttcgtattt ttactgctgc ctctttcatg
      241 ctggctcttg accgctcttt attttttcta tctatggcta tataaccgct taaagacatg
      301 tgccatccca tgaatggtat tttgaatagt tctttttttg ccgtccagcc aaactttacc
      361 ggaataatgg cactcaaaag ccatatatca aaaaaacttt gatgattgga tgcaaatatc
      421 tgaacatgtt cttttgaaat attttcaata ccctccacct gatatcgtat cccacaaaac
      481 catagtatat tctttgacca gttttgcatg caaaaatgag caaggtcacc tctggggctg
      541 acaaaccatg aaaaaagcga gatggttccc cagaatgcgg tatcaacgcc aatgaataac
      601 cacgttgcat aggtgtaacc cttgcgtatc acagtattca tataaacctc cgcaaatcaa
      661 catattttga tttatgaaca tatttgtgaa aacgcatgga ttacatcgtc tacaaaggga
      721 tttgtcaact aataaatcac aaccccatca ttctggatac aatcatcttc ataacttcag
      781 atgtaccagc ataaatagtt tgtatacgag catccacata aaatcgtgat accgggtatt
      841 ccagacaata cccataccct ccaaaaagct gcaagcaacg gtcagcaaca cgtttggcag
      901 tttcacatat ccagtattta gccatacagg tttctttcac tacatcatga ccctgtatat
      961 ggtgatgtat cagatcatca ataaagcttc tgccagctgc aatttccgaa gccatttcgg
     1021 caatggtaaa ctgcgtattc tgaaattttg aaagtggttt cccaaataac tttcgctctt
     1081 ttacataatc gatagttatt gccaaacatt tttccatggc tgcttgtgaa gttaacgcac
     1141 ataccagcct ttcctgttgc agcttctgca taagataaat aaatccctgt ccttcctgtc
     1201 ctaaaagatt attgtcaggg atgcgacagt tatcaaaaaa tagttcggct gtatcctgcg
     1261 ctttaagacc aatctttttg atgggtttcc ctctggtaaa gccttcagtc ccatcttcaa
     1321 ctaaaaaaag gcttaccgac tgatggggag gtaactcttt tgacgacctg gctgcaacta
     1381 taaccagatc tgccaggata ccatttgata taaatgtttt ctgaccattg agcacccagc
     1441 tatcgccatt ggaaacagca ctggttttca tgcctgcaag atccgaaccc gcctccggtt
     1501 cggtcattgc aatggcaagc actatctccc ctgaagcgca ttttggcagg tatttcatct
     1561 tttgttcttc tgtgccaaat gagtatatat atggcgcaac aatatcattg tggagtggaa
     1621 tcataaatga attagcaccg ctgtatgcca gctcctcatt gagaataacc gaatagagga
     1681 agtcgccacc tggaccgcca tattgttcct caagccatgg aagaagcaaa ccaagctgac
     1741 cgcagcgatt ccacatctca cgaggcacaa tgccttcatg ttcccatgta tcgtaaaaag
     1801 gcattacctg ttgctcaaaa aacttgcgta cggtttgcct gaacagagca tgctcctgcg
     1861 taaactgaat tgaacgttcc atttatttaa tccttataaa atcgcttatc cgtttttgcc
     1921 atatctgcca gtatcattgc aggtttaaaa cgctccagac ccctgttgta gtattgcaac
     1981 atggtatcaa caatggtacc ggcaccttca tggtcaatat atctaaatat tccgccctta
     2041 aatggtggaa aacccaatcc cagaacggaa ccaacatcgc cgtcacgcgg tgaggagatt
     2101 ataccttcct gcaagcagtg agctgcttca tttatcatca taaggcttac ccgctgttga
     2161 atctcttcta ctgatattgc ttctggagtt actttcccta tgaccgtgta cacttcttta
     2221 ttgggcaaac gcatgccttt tttcttagga ctatcgtaca tataaaatcc ttttttattt
     2281 tttttgccct taaagccttt gcttaaaagc aacggcagaa cattggacgg tgcagcaccc
     2341 cgcgcggtaa acatctgtcc taattcaatg gtaacatggg caccaacatc tataccaacc
     2401 tcgtccagca gtgctatggg accaatcgga tagccaaacg caagcatagc gctatcaata
     2461 tcattcattg gtacaccttc ttcaatcaaa acaacactct cattcagata cggtgctaat
     2521 attctggttg tataaaaacc aggaccatcc tttaccacaa tacaactttt tccctgagct
     2581 atcccggttg caacagctgt cgccaccacc caatctgctg tttttgcggt ttttattatt
     2641 tctaacaatg gcatggcagg tacgggtgaa aagtaatgca tccctatcat attctgcgga
     2701 cgctttgatg ctttcgcaat aaggctgata ggaattgccg aggtatttga tgcaacaata
     2761 gtggtatcgc cgcaaacagc ctcaacctgt tttaccagat tttgctttaa ttgcaaatct
     2821 tcaaaaacgg cttcaatgac caaatcagta tttttaaaat aggtataatc atcgcatgga
     2881 ataagttttc cgtacattac ctccttgtca aaattacgga tagcccccga ctgcgcccgc
     2941 ttttccaggc tttttctaat ctgtgccatg ctgcgtgtaa caaaatcaag cgaggtgtct
     3001 tttaacagca ccgtatcgca cagcggcaat gaaacgaaag caataccgct acccatgagc
     3061 cccgttccaa tgacggccag ctttgacaca tttttttcca tttttttaaa cggatttttc
     3121 tttaaatcgg tcatggcaaa aaagaggttt acaagtgatt gtgattgata actttgaaca
     3181 agatgaataa aacgctcact gtcctttgca atgccttttg cgatagttgt tttataacca
     3241 tattcaacag cgtcaatgac agcaacagca gccggatata acccccgtgt ttgcctcatc
     3301 accatggtgc gtgcctttga aaatatgata ctgcgcccta acggatttga ttctaaaagc
     3361 cactcagcaa atgttctttt tcgttttatt gttcgtttac cttttagctg taataccttt
     3421 ttcactgcta tttcttttaa tccatacggc ataactatct catcaataat cccaagccgt
     3481 tttgcacgcc ttgcccgtat agttgatccc tgcacaatga ggggaagtgc atgttgaatc
     3541 ccgattaatc gtggaagacg ttgtgttcct cccgcagcag gaataagtcc taatttaatt
     3601 tcaggaaatc ctatcacggt gtttgtggaa tcagttgcaa tgcggtaatc ggtaaccagt
     3661 gccagctcca caccgccacc cagacagttg ccattgataa cacaaactgt tgggaaggga
     3721 agtttctcta atccggacaa aacactattt gcttttgtaa tatacgctct aatctcatct
     3781 tcagttttca tggatgtaag ttcatcaaga tcagccccta ccacaaaatt gtcatccttc
     3841 ccgctaacta tcgccaaacc tttatatttt ttgctcttta catcattaat aattacttcc
     3901 atttcattga gcatagcact ggaaacttta ttaactttgc cgggacaatc tattgttact
     3961 accagcacat cgttcaaaac ctcaggttta caataggtga atgcgttcat tgcagtcctc
     4021 cttatgatcg ttctaatata attgcgttgc caatcgcacc agcagcacac ccggcaacaa
     4081 caccgtaacg cttgccactt tcctgcaaac gattggcaca tgttgttaca aggcgtgcac
     4141 ctgttgcacc aaatggatgc cccagcgata gcgaaccgcc atagatgtta aggtcatcat
     4201 gattaatctc acctacttta ccggacaatc ccagcctgtt tttgcaaaac gagtcagagg
     4261 caaggcattg cactacagca accatctgaa cgccaaatgc ctcatgaatt tcccacactc
     4321 caatgtcttt aaattttaat ttacccattg ccagtgcttt tggtatggaa aagacaggac
     4381 caagtaacag ctcttcccat aaatcctgag ccgtaaaggc atacgatttg atataggcaa
     4441 gcggttttaa tctcattttt ttagccagcg tttcactcat caaaagcact gcagcgccgc
     4501 catcggttaa aaatgaagag ttgccggcag ttactgtacc gtagcgctta tcaaatgcag
     4561 gcttgagtgc agctaatttt tcagctcttg tcccttttct gaaaccatta tctttgtcta
     4621 ccagtctgcc ctgcggggtt acaaccggca caacctcttt ttttaatatc ccttgttccc
     4681 atgctgctgc agctcggtta tgcgacagcg cagcaaatgc atcctgctcg cgctgtgata
     4741 tccccagcct ttttgccagc ctatcagcag tatcacccat acgcagttga gtggaaaact
     4801 cacctatttc aggttgttcg ggaacgataa aatctttaaa gctcatgccc tttaacagtt
     4861 taagctttcc cacaatagtt ctgggacgtt taaacatggt taagtccata ataaattggc
     4921 gatagttctt tgaaat
//
LOCUS       DAOSOX010000177         4907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17347_length_4907_cov_9.105936, whole genome shotgun sequence.
ACCESSION   DAOSOX010000177 DAOSOX010000000
VERSION     DAOSOX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17347_length_4907_cov_9.105936"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLX22_10440"
     CDS             <1..1475
                     /locus_tag="PLX22_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT20354.1"
                     /translation="VSVWATNVPEWVYLQFSLGMIGGVLVTVNTSYKSHELEYQLKQS
                     DSTTLFMIEGYRDSNYHQIVRELIPNLDCAQPGMINEPKLPMLKNIVYIGKRDATPGM
                     FTFHDMVMMGNAISDAALDKRISECDVDDVINMQYTSGTTGFPKGVMLTHYNIVNNGL
                     MVGDVMGTSPHDKLLIHVPLFHCFGCVMSTLNCVTHGAAMVVMESFDPLKSLQAVASE
                     KCTAINGVPTMFIAMLNHPDFDSYDMSSLRTGIMAGAPCPVETMNQVRTRMHCPDIVI
                     AFGQTESSPVMTMTRRDDPVSLRVSTIGRLLPDIEGKIVDPETGEDLPPNIQGEIVTR
                     SACVMKGYYKMPEATANAIDKDGWLHTGDLGSVDENGYFKVTGRIKDMIIRGGENIYP
                     REIEEFLLTNQNIQDVQVVGIPDEKYGEQVLAVIQLKEGQSSSAEEMVAFCTGRIARH
                     KIPKYWEFVKAFPMTASGKVQKYKLREMFASKYGMAASVF"
     gene            1503..1877
                     /locus_tag="PLX22_10445"
     CDS             1503..1877
                     /locus_tag="PLX22_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT20355.1"
                     /translation="MTTYSIGELAKLLEMSTRTIRYYEELGLLNSVKRIDNGRRIYTD
                     DDVRRLKLIKRLKILGLTLSEMHELEYIWQIHKTNDIVLKRLLEILDLHIRRIDDRMK
                     DLEILKNEIGEYKSRIKNKING"
     gene            complement(1879..2610)
                     /locus_tag="PLX22_10450"
     CDS             complement(1879..2610)
                     /locus_tag="PLX22_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT20356.1"
                     /translation="MGEKITIINLHKSFGQQVVFSGLHLSVYAGEILCIIGKSGSGKS
                     VLLKHCIGILKPDSGSILVDGQKISNDDSDASIIKKFGVLFQGGALFDSMNVYDNIAF
                     PLRRQGASEEIIAQKIPELLAHVGLKGIEARMPSELSGGIQKRVALARAIAKNPEIML
                     YDEPTTGVDPINSGSLYRLIAYMRKIFHVTSVIVTHDMRLAFTIADRIAMLHDGTIIV
                     EGTPQQIKECQDAVVQQFVSGKAKV"
     gene            2909..4186
                     /locus_tag="PLX22_10455"
     CDS             2909..4186
                     /locus_tag="PLX22_10455"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT20357.1"
                     /translation="MKVRCGLIGFGTVGSGVYHLLEKHRGDILKKTGIDIELVKICDV
                     RNIASMVAIPVTDKWEDIIYDTSIDTVIELIGGTEPAKSIIVSALKAGKNVVTANKKL
                     LAENGDEIIAASMSVKGSLAFEAAIGGGIPCVAALRNGLVGNSIQSVVGILNGTTNYI
                     LTRMSEDDMSFAEALALAQDKGYAEADPTFDIEGYDAAHKIVLLSMLAYNVGVSMKSV
                     YVEGITNITALDITYAHEMGYTIKLLGVSRMLDCKLDIRLQPVMIPLTNPLASVRNEF
                     NAIMYHGDMTGPVTLYGKGAGSFPTASAVVSDIVSIAQKQNGGKDCIFIEKDAEYISP
                     EYRMLRYYIRLYSEDRAGILSKIAGVLGGYNISIASVIQKEAMTEYVPIVITTHRANE
                     AGMRKAKNEIERFNFVNGHVMIIPIEDFDNTGE"
     gene            4189..>4907
                     /locus_tag="PLX22_10460"
     CDS             4189..>4907
                     /locus_tag="PLX22_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOT20358.1"
                     /translation="MGGDSNYRAVFRCIAGCSEEYPLDEVVYRCKKCGGLLEVYHDRD
                     RLRELSPKKWKELFNSRVGTTKWPYGSGVWSKKEWVCPEVDNENIVSMFEGNTNLFWA
                     KRFGAMIGMHDLWIKMCGNSHTGSFKDLGMTVLVSMVNEMIMRGKPIKAVACASTGDT
                     SAALAAYCAYAGIPSIVFLPRNKISTAQLVQPMSNGSIVMSLDTDFDGCMRIVQEITK
                     DNTIYLANSMNSLRIEGQKSIS"
BASE COUNT         1448 a          878 c         1182 g         1399 t
ORIGIN      
        1 atgtttcggt atgggcaacc aatgtacctg aatgggtata tttgcagttt tcactgggta
       61 tgattggcgg tgtgctggtg acggtgaata ccagttataa gtcgcatgaa ctggaatacc
      121 agttaaaaca atcagattca acaacgctgt ttatgattga aggatacaga gatagcaatt
      181 accatcagat tgtacgggaa ttaatcccta atttagattg tgctcaaccc ggtatgataa
      241 atgagccaaa actgccgatg cttaaaaata ttgtttatat tggaaagcgc gatgctactc
      301 ccggaatgtt tacctttcat gacatggtaa tgatggggaa tgctataagt gatgcggcac
      361 tggacaaacg aatcagcgag tgcgatgttg atgatgttat caatatgcag tacacatccg
      421 gtacaacagg ttttccaaag ggtgttatgt taactcatta taacatagtc aataatgggc
      481 taatggttgg tgatgttatg gggacatcac cccatgataa gctgctcatt catgtcccgc
      541 tgttccactg ttttggatgc gttatgagta cattaaactg tgtgacccat ggtgctgcga
      601 tggttgttat ggaaagtttt gatccgctga aatcgttgca ggctgttgcc agtgaaaagt
      661 gtacagccat aaatggtgtt cccacaatgt tcattgcaat gcttaaccat cccgattttg
      721 acagctatga catgtcatca ctgcgaactg gtataatggc aggcgctccg tgtcctgttg
      781 aaactatgaa tcaggttaga acacgcatgc attgtccgga tattgtcatt gcatttgggc
      841 aaaccgaatc ctcgcctgtt atgacgatga cacgccgtga tgacccggtg agtctgcgtg
      901 tgtcaactat tggcagactg ctaccggata tagaaggtaa aatcgttgat cctgaaacag
      961 gtgaagattt gccacccaac attcagggcg agattgttac acgcagtgcc tgtgtaatga
     1021 agggatacta taagatgcca gaagctactg caaatgcaat tgacaaagac ggctggttgc
     1081 atactggcga tttaggttcg gttgatgaaa acggctattt taaggttacc ggacgaataa
     1141 aggatatgat tatccgcggt ggtgaaaata tctatccgcg tgaaattgaa gaatttttgt
     1201 tgacgaatca aaatattcag gatgtacagg ttgtaggtat tcctgatgaa aagtatggcg
     1261 agcaggtttt agctgtcatc caattaaagg aaggacaatc gtccagtgca gaagaaatgg
     1321 ttgctttttg tactggcagg atagctcgcc ataagatacc aaaatactgg gaatttgtaa
     1381 aagcattccc catgacggca agtggtaagg ttcagaagta taaattacgt gagatgtttg
     1441 catcaaaata tggcatggca gcttcggttt tttaaaaata ataacaggtg attgaagtaa
     1501 aagtgacaac ctattcaatt ggtgagttag caaagctttt agagatgagc acgcgcacca
     1561 tacggtatta tgaagagctg ggactgctca actcggtgaa gcgcatagac aatggcagac
     1621 gtatctatac tgatgatgat gtcaggcgct tgaaattaat caagcgcctg aagattcttg
     1681 gattgaccct ttctgagatg cacgagttag agtatatctg gcagatacat aaaacaaatg
     1741 atattgtgct taaacgctta ttggagattt tagatttgca catcaggaga attgacgaca
     1801 ggatgaagga ccttgaaatc ctgaaaaacg agattggtga atataaaagc aggattaaaa
     1861 ataaaattaa tggataattt atacttttgc ttttcctgaa acaaactgtt gtacaactgc
     1921 atcctgacat tcctttattt gctgtggcgt tccctcaaca atgattgtgc catcatgaag
     1981 cattgcaatg cggtcggcaa tggtaaaggc aagcctcata tcatgggtaa ctatcaccga
     2041 agtaacatgg aatatctttc gcatgtatgc tatcagcctg tacagcgaac ctgagtttat
     2101 gggatccacg ccggtggttg gttcgtcgta cagcataatt tcaggatttt tggcgatagc
     2161 tcttgcaagc gccacccttt tctggatgcc gccggaaagc tctgaaggca tccttgcctc
     2221 aattcccttt agcccaacat gggcaagcaa ttcagggatt ttttgtgcga ttatctcctc
     2281 gcttgctccc tgcctgcgta agggaaaggc aatattgtca tatacattca ttgagtcaaa
     2341 cagcgcaccg ccctggaaaa gaaccccaaa tttctttata atagatgcat cgctatcatc
     2401 attggaaatt ttttgaccat caacaagtat gctgccgcta tcaggtttta aaatgccaat
     2461 acaatgcttt agcagtactg attttcccga accgcttttg cctataatac acagtatctc
     2521 acccgcatag accgaaagat gaagcccgga aaaaaccacc tgttgtccaa atgatttgtg
     2581 gaggttgata attgtaattt tttcacccat ggtactatta tagtataatg gcatattctt
     2641 ataaagtaaa aaattaatat aaactgcatg gatgccgttt tatacgtttg agtgcagcac
     2701 catgattctt cgctttgctt tgaatgacag acactttgtt tagaatgact atcgccagat
     2761 attgttaact attatgggca gacaaaaatc ggctgtcaaa aacctgtcaa catatatgct
     2821 gcataagtca atacctataa ataaagttga caattaaact gttgccggat tatgaatgac
     2881 tatcatctat aaaaaatgag gaaaggctat gaaggtgcga tgcgggctta ttggttttgg
     2941 tactgtggga agtggtgttt atcatctttt agaaaaacat cggggcgata ttttaaaaaa
     3001 aacaggaata gatattgagc tggtaaagat atgtgatgtg cgcaacattg cttctatggt
     3061 tgccatcccg gttaccgaca aatgggaaga catcatatat gacaccagca ttgatacggt
     3121 gatagagctt attggtggaa cagaacctgc taaatctatt attgtttcag cactgaaggc
     3181 gggtaaaaat gttgtaacag caaacaaaaa gcttttagcc gaaaatggtg atgaaataat
     3241 tgcagcaagc atgtcagtaa aagggtcact ggcatttgag gctgccattg gtggtggtat
     3301 accctgcgtt gcggcactgc gcaatgggct ggttgggaat tccatacagt cggtagtggg
     3361 aatactaaat ggtaccacca attatatatt gacacgcatg tcagaagatg acatgtcgtt
     3421 tgccgaggca cttgcattgg cacaggataa agggtatgct gaagctgatc caacctttga
     3481 tattgaaggg tacgatgcag cgcataagat tgtgctgcta tcgatgttag cctataatgt
     3541 tggtgtttcc atgaagtcgg tttatgttga aggaattacc aatatcaccg ctctggacat
     3601 tacgtatgct catgagatgg gatataccat caagcttctg ggtgttagcc gtatgcttga
     3661 ttgtaagctt gacatacggc tgcagccggt gatgattccg cttactaatc cgctggcttc
     3721 ggtgcgcaat gaatttaatg ctattatgta tcacggtgat atgaccggcc cggtgacgct
     3781 gtatggcaaa ggtgccggaa gcttccctac agcatctgcg gtagtcagtg atattgtttc
     3841 tattgctcag aagcaaaatg gtgggaagga ttgtatattt attgaaaagg atgcagaata
     3901 tatatcgcct gaatacagga tgctgaggta ttatatacgg ttgtattctg aggatagggc
     3961 aggcatactt tcaaagatag ctggtgtact gggagggtat aatatttcaa ttgcttcggt
     4021 aatacaaaag gaggcaatga ctgaatatgt tcctattgtc atcacaacgc atagagcaaa
     4081 tgaagcagga atgcgaaagg caaaaaacga aattgaacgg ttcaattttg ttaatggtca
     4141 tgtgatgatt attccaatag aagattttga taatacaggt gaatagttat ggggggcgac
     4201 agtaactata gagcagtttt tcgctgtata gctggttgca gtgaagagta tccattagat
     4261 gaggttgtat acaggtgcaa aaagtgtggt gggctgttag aggtttatca cgatagagac
     4321 cgtttacggg aactatcgcc aaaaaaatgg aaagagcttt ttaatagccg tgttggcacc
     4381 acaaaatggc catacgggag cggcgtctgg agcaaaaagg aatgggtgtg ccctgaggtt
     4441 gacaatgaaa acattgtttc catgtttgaa ggtaacacaa atttattctg ggctaaacgc
     4501 tttggtgcta tgattggcat gcatgatctg tggattaaga tgtgtggcaa cagccatacc
     4561 ggttcattta aggatttagg gatgacggtg ctggtatcca tggttaatga aatgatcatg
     4621 cgggggaagc caattaaagc ggttgcatgc gcttcaaccg gtgatacatc ggcagcattg
     4681 gcagcatact gtgcatacgc cgggatacca tcgattgtat ttttaccacg gaacaaaatt
     4741 tctaccgctc aactggtgca gcccatgagt aatggctcta tcgtcatgtc gcttgatact
     4801 gactttgatg gttgcatgag gattgtgcag gagataacaa aagacaatac catctatctg
     4861 gctaattcta tgaattcgtt gcgtatagaa gggcagaagt caatctc
//
LOCUS       DAOSOX010000178         4865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17506_length_4865_cov_7.855717, whole genome shotgun sequence.
ACCESSION   DAOSOX010000178 DAOSOX010000000
VERSION     DAOSOX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17506_length_4865_cov_7.855717"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLX22_10465"
     CDS             complement(<1..233)
                     /locus_tag="PLX22_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT20359.1"
                     /translation="MAFKRLTIGKALYDTVEKYPDHEALVCPEFGVRLTYKQFYHKCR
                     EVARGLIALGVKKGDHVSVWATNVPEWVYLQFSL"
     gene            707..1129
                     /locus_tag="PLX22_10470"
     CDS             707..1129
                     /locus_tag="PLX22_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT20360.1"
                     /translation="MDYTILTAPCGLDCFNCPMYLAANDGSLRIKIAKSMNISDEQAV
                     CKGCRNENGTIGFLNMKKQCTVYACTTSKGIHFCHECDDFPCDNLHPYADRASQVPHN
                     TKVFNLCLIKKMGLEKWAQEKAKDVKKIYFGGKFTLTE"
     gene            complement(1185..1964)
                     /locus_tag="PLX22_10475"
     CDS             complement(1185..1964)
                     /locus_tag="PLX22_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOT20361.1"
                     /translation="MQLKDFKEELTTVWSFPERGKWATHNAKYRGNYAPQVARNLIQR
                     YTEQYDWILDPMVGSGTSLIEAKLLCRNIIGIDINPYAIELSKKALSFEVEDGNCKQE
                     AYVGDARKLSNISDESIDFIIFHPPYLNIIKYSQGVIKEDLSNISNVGKFFDEIEIIA
                     KELLRVLKHDKYCAVLIGDTRKGQHYIPLSYFLLQRFLKLGFILKEEIIKTQHNCVYS
                     KRWEENAKRFGFYLIMHEHLFVFRKPKLGENLLKIRWSCHI"
     gene            complement(1966..3309)
                     /locus_tag="PLX22_10480"
     CDS             complement(1966..3309)
                     /locus_tag="PLX22_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20362.1"
                     /translation="MESFSVYMKTLLEKIRNSLDDDLSIDEVRFIISKTNEYFFTYKE
                     QLGQTFILDEYYEYFSEFHKFWEKYHRKILNPSIDENNCEQVANVLKKLYESENGQKL
                     FYELYDTKNLKPEEICQIRYFSANQDFRGSRDFEDLFLIYSSDPTIFDKQKIYDNPED
                     FLKSIGITSLSQNDKRVKYAKTAAEILIKLNIDAFNLFEHFESDIFKIRNFLITDNRG
                     SGFGNKKTDMFLRDMVVLGVWKNPKNFEKIDVASDINTIKVALRTRILKTEIPLISSF
                     MDIFCYQYSLIDEMNALAWRKVWEIWKNKYPESCIESPCLMDYFIYRIIGREFCKESL
                     CIFECEAEKHAFRWHSSRNRTCQICKKSKATIIKKVLPCTDSEGYIAIQKNVFVTGND
                     AIFPNVNECPFVVVCNPKSENFKKLNPPKSISILGQTGWESARTKAEEGGGGLMS"
     gene            complement(3323..4246)
                     /locus_tag="PLX22_10485"
     CDS             complement(3323..4246)
                     /locus_tag="PLX22_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOT20363.1"
                     /translation="MIDEISMLSRPFIKWAGGKSQLIDEIDERLPVELKERKITTYFE
                     PFLGGGAVFFYIAQKYSIERAILFDINADLIITYKVVKYNPEELMRFIDYYKSFYNSL
                     DIEQQKDFFYRIRQTYNKNKVKINFNDYNNGWIEHAAQMIFLNKTCYNGLYRLNSKGE
                     FNVPFGKYKNVKFYDKENILYSSALLQKAELYICDFEEIIHYDVNNAFIYFDPPYRPI
                     SQTASFTSYSKFDFSENDQIRLAKIFKELDKRNARLMLSNSDPKNVNLNDDFFEKHYK
                     GFNIQRVYANRMINCNGEKRGKINELIITNY"
     gene            complement(4358..4687)
                     /locus_tag="PLX22_10490"
     CDS             complement(4358..4687)
                     /locus_tag="PLX22_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20364.1"
                     /translation="MSVHFKHPQLEHEVESISGVYAYTYEKRLPVDDKEILCFIGYAI
                     ANKSCCGVGGCMFATVAGFLQEYAVQKEGDSIISVVEPVSDTSTRQRIEAMLRLEGIQ
                     QVQFHAA"
BASE COUNT         1597 a          906 c          615 g         1747 t
ORIGIN      
        1 agtgaaaact gcaaatatac ccattcaggt acattggttg cccataccga aacatgatca
       61 ccttttttta cacccaatgc aattaatcct ctggcaacct ccctgcattt gtgataaaac
      121 tgcttatacg tcagccttac cccaaattca ggacatacca atgcctcatg atctggatat
      181 ttttccacag tatcataaag cgccttccct atcgttaatc tcttaaaagc cataccaacc
      241 tctccttttt taaaatatat atagcttata ataatacaac aaaataattt ttataagacc
      301 agtttacgta aactgttata tgactgcatg gtatgtcaac ataattacat aattttttat
      361 caatttttga aatttacaaa acagcagaca ctacctgccc ggtatacaac cctaccactc
      421 ttttttttat tctttgtgcg atagttactg cttattgaca ttttgtgcaa taaggtatat
      481 cctgtcaata tttaattggc tgtccaatca attttattaa tgtattctgc accatcaaat
      541 atatttttgc cagataaaaa ttatagtgca atcaatgtat tatataaaca atcatgtttt
      601 acatgaatca taacaaacct tgcatacaca ttttatggaa ggcgttgtaa caggcaaagt
      661 ataactgtca ttgagtaacc tgattacatt acaacaagga ggttttatgg attatactat
      721 tttgacagca ccatgcggat tagactgctt taactgcccc atgtatctgg cagcaaatga
      781 tggcagcctt cgcatcaaga ttgcaaagtc tatgaatatc agtgatgaac aggcagtgtg
      841 caagggttgc cgtaatgaaa acggaacgat tggtttttta aacatgaaaa agcaatgcac
      901 ggtatacgca tgtacaactt caaaagggat acatttttgc catgaatgtg atgatttccc
      961 ctgtgataac ctgcacccct atgccgacag agcatcacag gtaccccata atacaaaggt
     1021 tttcaacctg tgccttataa aaaagatggg tcttgaaaag tgggcacaag aaaaggcaaa
     1081 ggatgtgaag aaaatctatt ttggagggaa atttacactg acggaataaa ttgagtggta
     1141 ctattactgt agtatattta tgtgaattaa tgtggcatcc ggatttatat atggcaactc
     1201 catcgtattt ttaataaatt ttctcctagt ttaggtttcc taaacacaaa caaatgttca
     1261 tgcataatta aataaaaccc gaatcgtttt gcattttctt cccatctttt tgaataaaca
     1321 caattatgct gtgtttttat tatttcttct tttaagataa atcccaattt taaaaatcgt
     1381 tgtaataaaa aatatgaaag tggtatatag tgctgacctt tccttgtatc gcctattaac
     1441 actgcacaat atttatcatg tttcaagacc cttaataact ctttggcaat aatttcaatt
     1501 tcatcaaaaa atttaccaac attagaaata tttgataaat cctctttaat gacaccctga
     1561 gaatatttta ttatatttaa atatggtgga tgaaaaataa tgaaatctat tgactcatca
     1621 gaaatattag acagttttct tgcatcacct acataggctt cttgtttaca attaccatct
     1681 tctacctcga agcttaaggc tttttttgat agttcaatag cataaggatt gatatcaatt
     1741 cctataatat tcctacataa aagttttgcc tcaattaatg acgttccaga ccctaccatt
     1801 ggatcaagta tccaatcata ttgctctgta tatctttgta tcaaatttct tgctacttga
     1861 ggtgcataat tacctctata ctttgcatta tgcgtagccc attttcccct ttcaggaaat
     1921 gaccatacag tagttaattc ttctttaaaa tctttaagct gcatattatg acattaaacc
     1981 tcctcctcct tcttcagctt ttgttcttgc actttcccag ccagtttgtc ctaaaatact
     2041 aattgatttt ggcggattta attttttaaa attttcactt tttgggttac atacaacaac
     2101 aaacggacat tcattgacat ttgggaatat agcatcgttg cctgtaacaa aaacattttt
     2161 ttgaatggca atatatcctt cactatccgt acatggtaaa acttttttaa taattgttgc
     2221 cttacttttt ttacaaattt gacatgttct gttccttgac gagtgccacc gaaaagcatg
     2281 tttttctgct tcacactcaa aaatgcataa agattcttta caaaactctc tacctattat
     2341 acgatatata aaataatcca tcaaacaagg actttctata caagattctg gatatttatt
     2401 cttccaaatt tcccacactt ttctccatgc caatgcattc atctcatcaa ttaaactata
     2461 ttgataacaa aagatatcca taaaacttga tatcagtgga atttctgttt ttaaaattct
     2521 tgtgcgcaag gcaactttaa ttgtgttgat gtcacttgcc acatctattt tttcaaagtt
     2581 ttttggattt ttccaaacac caagtactac catatccctt aaaaacatat ctgttttttt
     2641 attaccgaat ccacttcctc tgttgtcagt tataaggaaa tttcttattt taaaaatatc
     2701 gctttcaaaa tgttcaaata aattaaaagc atcaatattt aactttatta atatctcagc
     2761 agcagttttt gcatacttta cgcgtttatc gttttgtgat aaacttgtaa tgccaatact
     2821 ttttaaaaaa tcttcgggat tatcataaat tttttgctta tcaaagatag taggatcact
     2881 tgagtaaatt aaaaataaat cttcaaaatc acgagaaccc ctgaaatcct ggttagctga
     2941 aaaatatcta atctggcata tttcttctgg tttaagattt tttgtatcat aaagttcata
     3001 aaaaagtttt tgaccatttt ctgattcata taattttttt aaaacatttg caacttgctc
     3061 acaattattt tcatcaatag aaggatttaa aattttccta tgatattttt cccaaaattt
     3121 gtggaattct gaaaagtatt cgtaatattc atccagaata aatgtttgac ccaattgttc
     3181 tttatatgtg aaaaaatact cattagtttt tgatataata aatcttacct catcaatact
     3241 aagatcatca tctaaagaat ttcttatttt ttctaataat gttttcatgt aaacagaaaa
     3301 actttccatt aaccatctcc aatcaataat tagtgataat caattcgtta atctttcccc
     3361 ttttctctcc attacaattt atcattctat ttgcatatac tctttgaata ttaaatcctt
     3421 tataatgctt ttcaaaaaaa tcatcattga gattaacatt tttagggtca gagttactta
     3481 acataagtct tgcatttctt ttatctaatt ctttaaatat tttagccaat cttatttgat
     3541 cattttcact gaaatcaaat ttactatatg aagtaaaact tgctgtctga cttatagggc
     3601 ggtatggtgg atcaaaataa ataaacgcgt tgttaacatc ataatgtata atctcttcaa
     3661 aatcacaaat atatagctct gccttttgta ataatgcaga actatataat atattttcct
     3721 tatcataaaa tttcacattt ttatatttcc caaaggggac attgaactct ccttttgaat
     3781 ttaatcgata taatccatta taacatgttt tatttaaaaa aatcatctgg gctgcatgct
     3841 caatccatcc gttattataa tcattgaaat tgattttaac tttatttttg ttataagttt
     3901 gtcttatgcg gtaaaaaaaa tctttttgtt gttcaatatc caaactatta taaaaagact
     3961 tataataatc aatgaaacgc attaattctt caggattata tttgacaact ttatatgtta
     4021 ttatgagatc tgcattaata tcaaataaaa tagccctttc tattgaatat ttctgtgcaa
     4081 tataaaaaaa cacagctcca ccacctaaaa aaggctcaaa atatgtagtt atttttcttt
     4141 cttttaattc tactggtaat ctttcatcta tttcatcaat aagttgcgat ttcccaccag
     4201 cccatttaat aaatggcctg gataacatac taatttcatc aatcatccct ttcccttaaa
     4261 attgaacaaa tttataaaat agtaaataat ttacaataag cttctctgtt gtaatatcaa
     4321 cacatttttt ataaatgaag gcataacgca acatagttca tgccgcatga aactgtacct
     4381 gctgtatacc ctccaaacgc aacattgcct caatcctttg acgagtgctg gtatcgctaa
     4441 caggttcaac cactgaaata atactatcac cctctttttg taccgcatat tcctgtaaaa
     4501 atcctgcaac ggttgcaaac atacatcctc ctaccccgca acaactttta tttgcaatgg
     4561 catatccaat aaaacataat atctctttat catcaacagg cagacgtttt tcataggtat
     4621 aggcatacac gcccgaaatg gattcaacct catgctccag ttggggatgt ttaaaatgca
     4681 cactcattgt tacctgcagc aactaccatt caatgagcac aggttgctgt gttcaacctg
     4741 caatgttgtg tgggtaatat taaatttttc ttcaagcatg gcatttattt ttgttatcag
     4801 cacatcagtg ttagagtaac tatcgccctt atcaatacac aggtgacacg ataaaaacac
     4861 ctcat
//
LOCUS       DAOSOX010000179         4817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17699_length_4817_cov_9.155397, whole genome shotgun sequence.
ACCESSION   DAOSOX010000179 DAOSOX010000000
VERSION     DAOSOX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17699_length_4817_cov_9.155397"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..303)
                     /locus_tag="PLX22_10495"
     CDS             complement(70..303)
                     /locus_tag="PLX22_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20365.1"
                     /translation="MIYILTIYFGDYNGHNCSYVFSLTVTIEKIHPLYYSFENHALCN
                     YTITKGVLETQKYINKKLDKIPYFSGILKESSI"
     gene            complement(353..1084)
                     /locus_tag="PLX22_10500"
     CDS             complement(353..1084)
                     /locus_tag="PLX22_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20366.1"
                     /translation="MEQEFIRKGRQFVKSCAATLPLPRFYRDNYRYISIAQWIATASS
                     FVRYCSRCMYNKGTALGHSFYHAEKVAIESAAIVLKERGASEHSIHLAVLALMAGYFH
                     DYFREMSDHPSKAAEYVQNHFYHFIPEDDVLAISFAIQNHEAFKEHQQVDNDDLMLLS
                     NALYDADKFRWGPDNFLYTIWDMVATLHISHNDIVMHFSEGVAHIDTIRHTFRSKTGI
                     QYGPEFIDHGLVIAKQLKNYLEAKE"
     gene            complement(1118..2011)
                     /gene="hisG"
                     /locus_tag="PLX22_10505"
     CDS             complement(1118..2011)
                     /gene="hisG"
                     /locus_tag="PLX22_10505"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOT20367.1"
                     /translation="MSLIKLGIPKGSLENATIELFKHAGFTISVSSRNYFPSIDDDEI
                     SCSLVRAQEMSKYVESGVLDVGLTGLDWILENESDVEVISDLMYSKVSARKARWVLAV
                     PNDSPIKTVEDCAGKTISTELVNFTRKYFSERNIPVKVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIKAHGLKIIYTLLETNTKLIANKKSMANPQKRAKIKQIALLLKGALNASN
                     LVGIKMNVPEEKLDVVIKLIPSLTSPTVSKLYNATWFSVESVINESVVRDLIPRLINA
                     GADGIIEYPLNKVVSQEDVCL"
     gene            complement(2008..2391)
                     /gene="hisI"
                     /locus_tag="PLX22_10510"
     CDS             complement(2008..2391)
                     /gene="hisI"
                     /locus_tag="PLX22_10510"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOT20368.1"
                     /translation="MIQLDFTKLDGLVPAVAQDYKTGEILMVAFMNEEAFELTLKTGI
                     VHYWSRSRKTLWKKGESSGNVQQVKEIRIDCDNDCVLLKINQIGDAACHTGYRSCFFR
                     VVKGDELAIDGIKVFNPEDKYGDNK"
     gene            2698..>4817
                     /gene="ileS"
                     /locus_tag="PLX22_10515"
     CDS             2698..>4817
                     /gene="ileS"
                     /locus_tag="PLX22_10515"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT20369.1"
                     /translation="MDYSKTVNLPSSDFPMKANLPVREPHILQRWEKDNIYQLILKSR
                     EGNELYILHDGPPYANGHIHLGHALNKILKDIIIKHKTMTGFQAPYVPGWDCHGLPIE
                     LQISKELGDKAKTLPKQQIRKLCREYAQKFIDIQRDEFKRLGVFGDYHNPYLTMSSDY
                     EATIVEIFASLFELGFITKGKKPIYWCPTCVTALAEAEVEYHQHSSPSIFVKFKVDPS
                     SVSFKGVDSNNLYVVVWTTTPWTLPANLAVCFHPDFDYSAYKSNDEYFIMADGLAQSF
                     TSITGIQLGEKTPLTGEMIRLLKVYHPFIDRESKVILGNFVTLEQGTGIVHIAPGHGL
                     EDYIAGLEYGLDVFCPVDDEGKFTDEFAPMKGINVFDANPKIIELLQEKNALIFTNDI
                     EHSYPHCWRCKQPLIFRATAQWFLLIDHNDMRNIALQAIEDTQWIPEWGKLRFKGMVE
                     TRPDWCLSRQRSWGVPIPSFYCKQCGKNLMNADTIFYFAKVARERTIEAWYTDEIHSL
                     VPDGFTCECGSDSFEKEYDILDVWFDSGVSHFAVLDARDDLRWPADLYLEGSDQHRGW
                     FQSSLWPALALRQRAPYDTVLTHGFMLDDQGRAMSKSLGNVIPPEDIIDKFGADILRL
                     WVASEDYRNDVRIGYDMIQQIADSYRKIRNTFKFMIGNLSDFTATDCVLYEELSNIDT
                     WILHKVSVLSDQVRQHYEHYEFHLV"
BASE COUNT         1434 a          988 c          884 g         1511 t
ORIGIN      
        1 ctggtgacct tttgcttacc atgcaaatgc tctaccgact gagctacgtg ggcaaacctg
       61 aattaaacat tatatactac tttctttaag tatccctgaa aaatatggta ttttgtcaag
      121 ttttttatta atatacttct gcgtttcaag cacacctttt gttattgtat aattgcataa
      181 agcgtggttt tcaaaactat aatacaaagg atgtatcttt tcgatagtta ctgtcaaaga
      241 aaaaacatag ctgcaattgt gcccattata atcaccaaag tatatagtaa ggatgtatat
      301 cattcatcaa taagtcatca ataagcacag catacgttat caacaacatt cactactctt
      361 ttgcttctaa ataatttttt aattgttttg caatgaccag cccatgatca ataaattctg
      421 gaccatactg tatgccagtt tttgatctga atgtatgccg tattgtatca atatgtgcca
      481 ccccttctga aaaatgcatt actatatcat tgtgcgaaat atgcaacgtg gctaccatat
      541 cccatattgt gtataaaaaa ttatccggac cccacctaaa tttatcagca tcatacagtg
      601 cattagacaa aagcattaaa tcgtcattgt ccacctgttg atgctcttta aatgcttcat
      661 gattttgtat ggcaaatgag attgccagca catcatcttc cggaataaaa tggtaaaaat
      721 gattttgcac atattctgca gcttttgaag gatgatcgct catctcacgg aaataatcat
      781 ggaaatatcc tgccatgagt gccaatactg ctaaatgtat cgaatgttct gaggcccccc
      841 tctctttaag aactatcgct gctgattcaa tagctacttt ttctgcatga taaaagctat
      901 gccccaatgc tgtacctttg ttatacatgc aacgagagca ataccgcaca aacgacgatg
      961 cggttgctat ccattgtgct atagatatat accggtaatt atctctatag aaacggggta
     1021 gaggcaatgt tgcagcacat gacttaacaa actgcctgcc cttacgtatg aattcctgct
     1081 ccatgaaatt cctgtaacat tgaaatgtcg caggttatta taaacaaaca tcctcctgcg
     1141 atactacctt gttgagcgga tattcgatga ttccatcagc gccggcatta ataagcctgg
     1201 ggataaggtc ccggaccacc gattcattta tcacgctttc aactgaaaac catgttgcat
     1261 tatatagttt agataccgtt ggagacgtaa ggcttggaat aagctttata acaacatcaa
     1321 gcttctcttc gggtacattc attttaatac caacaagatt gctggcattg agtgcacctt
     1381 ttagcaatag agcaatttgt tttatctttg cgcgtttttg cggattagcc atactttttt
     1441 tattggcaat aagcttggta ttggtttcca gcaatgtata gatgattttt agcccgtgtg
     1501 ccttaattgt tgaaccggtt tcggtaacct caactattgc atcaacaagc ccttcaacta
     1561 cctttgcttc agttgcaccc catgaaaatt caactttcac aggaatattc cgctcgctga
     1621 aatatttccg tgtaaaattg acaagctcag ttgatattgt tttgcctgca cagtcctcta
     1681 ctgtctttat tggtgaatca ttgggaactg caagcaccca tctggctttc cttgcgctca
     1741 cctttgagta catcaggtcc gaaattacct ccacgtctga ctcattttca agtatccagt
     1801 caagaccggt caatcctaca tcaagaaccc cgctttcaac atattttgac atctcctgcg
     1861 cacgaacaag tgaacaggaa atctcatcat catcgatact tgggaaataa ttacgcgatg
     1921 atactgatat ggtaaatcct gcatgtttga aaagttcaat ggtagcattc tccagacttc
     1981 ctttaggaat acctaatttg ataaggctca tttgttatct ccatatttat cttcaggatt
     2041 aaataccttt atgccatcaa tggcaagctc atcccctttc acaactctga aaaaacagct
     2101 acggtatccg gtatggcatg ctgcgtcacc aatctgatta atttttaata acacacaatc
     2161 gttgtcacag tcaatacgaa tttcttttac ctgctgtaca ttgcccgagg attccccttt
     2221 tttccatagc gttttgcggg atctactcca gtaatgaaca atacctgttt taagtgtcaa
     2281 ctcaaatgcc tcttcattca taaaggcaac catcaaaatt tcacccgttt tataatcttg
     2341 agctactgct ggaaccaatc catccagttt ggtaaaatct aattgtatca ttagtatatc
     2401 ctgaatatat aaaatttttt cacaataact atcgccaaaa aaaacgttaa tggttactaa
     2461 actactgaca caccagtagt aaaactattt aatatctgct aataccataa aaacattttt
     2521 aacaatatcc ataaaattaa tatatacaaa aaacagaagt tatgtgatag tgtatcttat
     2581 atcacaggct gttataaaaa tcattaaaat gtatttataa attgctaaaa ttctattgac
     2641 aaatgtattt ttgcactatc actgaatttc acattactac tgtgttgcga ggtatcaatg
     2701 gattattcta aaactgttaa tcttccttca agtgatttcc ctatgaaggc taaccttccg
     2761 gtacgggaac ctcacatttt acaacgctgg gaaaaagaca atatctatca attaattcta
     2821 aaatcccgcg aagggaatga actgtatatt ttgcatgatg gtccaccgta tgcaaacgga
     2881 catatacacc ttgggcatgc attaaataaa attcttaaag atatcatcat taaacataaa
     2941 acaatgaccg gttttcaagc cccgtatgtc cccggctggg actgtcatgg tcttcctatt
     3001 gagctacaga tttcaaaaga attaggcgac aaagcaaaaa cattgccgaa acagcagata
     3061 cgaaaacttt gccgggaata tgcacaaaaa tttattgata tccagcgtga tgaatttaaa
     3121 cgattgggcg tctttggtga ctaccataat ccttatttaa ccatgtcatc agattatgag
     3181 gcaactattg ttgagatatt tgcatcgctc tttgagcttg gatttatcac caaaggtaaa
     3241 aagcctatct actggtgccc tacctgcgtt accgctttag ctgaagccga ggtagaatat
     3301 caccagcaca gctcaccttc aatctttgtt aagtttaagg ttgatccatc atcagtttcg
     3361 tttaagggag ttgattcaaa taatctttac gttgtagtat ggacaacgac accatggaca
     3421 ctgcccgcta atttagcagt gtgtttccat cctgattttg attattcagc ctataagagc
     3481 aatgatgaat attttattat ggctgatgga cttgcccaga gttttaccag tataacaggt
     3541 atacagcttg gtgaaaaaac acctcttact ggtgaaatga tccgcctgtt aaaagtatat
     3601 catcctttta ttgacaggga atcaaaagtg atattgggca actttgtaac tctggaacag
     3661 ggtaccggca ttgtgcatat tgcaccaggg catgggcttg aggattacat tgcagggctt
     3721 gaatatgggc tggatgtatt ctgtcctgtg gatgatgagg gtaaatttac cgatgaattt
     3781 gcaccaatga agggtatcaa tgtctttgat gctaacccaa aaataattga acttttacaa
     3841 gaaaaaaatg ctttaatctt taccaatgac atagagcatt cctacccgca ttgctggcga
     3901 tgcaaacaac cgcttatatt cagagctact gcacagtggt ttttgctcat cgatcataat
     3961 gatatgcgca atattgcatt acaggcaatt gaagatacgc agtggatacc tgaatgggga
     4021 aaattgcgat ttaaaggcat ggtagaaaca cgtcctgact ggtgcttatc ccgccaacgt
     4081 tcgtgggggg ttcccatccc ttcattttac tgtaaacaat gcggaaaaaa cctgatgaac
     4141 gctgatacaa ttttttattt tgctaaggta gcccgtgaac gcaccattga ggcatggtat
     4201 accgatgaga ttcatagcct tgtaccggat ggttttacct gcgaatgtgg tagcgatagt
     4261 tttgaaaaag aatatgatat tcttgatgta tggtttgact cgggcgtatc gcattttgct
     4321 gttctggatg cccgggatga tcttcgatgg cctgctgatc tttatcttga ggggagcgat
     4381 cagcatcgcg gatggttcca gtcttcactg tggcctgcac tggcactcag gcagcgagct
     4441 ccttatgata ctgtgcttac tcacggtttt atgctggatg atcagggaag ggcgatgagc
     4501 aaatcattgg gcaatgtcat cccgcctgag gatattattg ataaatttgg tgctgatata
     4561 cttcgcctgt gggtagcttc cgaggactat cgcaatgatg ttcgtattgg ctatgacatg
     4621 attcagcaga tagccgactc ataccgcaaa atacgcaata cctttaaatt tatgattggc
     4681 aacctttccg attttacagc aacagattgc gttttatatg aggaactatc gaacattgat
     4741 acatggatat tgcataaagt atcagtgctt tctgaccagg tacgacagca ttatgaacat
     4801 tacgaattcc atttagt
//
LOCUS       DAOSOX010000180         4812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17721_length_4812_cov_9.490856, whole genome shotgun sequence.
ACCESSION   DAOSOX010000180 DAOSOX010000000
VERSION     DAOSOX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17721_length_4812_cov_9.490856"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1462)
                     /locus_tag="PLX22_10520"
     CDS             complement(110..1462)
                     /locus_tag="PLX22_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOT20370.1"
                     /translation="MELKRQLGLFTGIMVIIADMIGTGIFMTTGNILGMTNNALIVIL
                     LWVLGGVVALCGALSYSELAVLWPDVGGEYIYLKKIFGNLPAFLTGWVSLIVGFTAPV
                     ATSSMLLVQYLNKFFINIHATGITTITDDIFWQKNIAALIIITFGCIHIMGVKRGSAI
                     QNILTVIKIAIVAAFIIAGFYVLDSSAIHRLYAHYGTVEAGSIPLIGLSLLMVMFAYT
                     GWNGATYIAGEIVAPEKNLPRIMFWGVTITTVLYILLNIVFLMSTDGINIMNHDEIGA
                     IAANALFGPVASNFFSITIAIILLSSVSAQMMLGPRVYYAMANNNMIFSLLSKISNRF
                     GTPLYAIVLQMILAVIYVYSGTAMSLVIYMGFALNVFPVLTVIGLMVIRKKHPELKSS
                     YRTPLYPLVPLVYVVLTIIMMIAALCCWTITSAFAIGVLLLGIPVYYIWQRYTTGKSS
                     "
     gene            complement(1474..4221)
                     /locus_tag="PLX22_10525"
     CDS             complement(1474..4221)
                     /locus_tag="PLX22_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20371.1"
                     /translation="MYLDSLEIFGFKSFADKIKIHFEKGITAIVGPNGCGKSNIVDAT
                     KWVLGEKQAKNIRGDKMEDIIFAGTDQRKPLSLAEVSLVINNTNRILHIDSDTVTVTR
                     RVFRDGESEYMINKSPVRLKDIEKLFLDTGLGKASYSVMEQGKIDLILSTKAEDRRYI
                     FEEAAGISRFKVQKKESLKKLEDTTVNLARLNDIIKEVEREKDLKSKQAEKTKIYFGL
                     REKYKRNDISLQLLRYRDFQKKLQKVTEDREKLFAEREELSKKVTLISSENENDEKRK
                     NDIQLQLFEWDKKLHTYTIKLEDIDGKTSRNKQLIQQQEQQITATRQRINEIDETVKK
                     LTEEKEKNIQQSQIIVNNIASDKQQLENLYTHRKKKIQNIHSFRDAIEQKKHAIKDFE
                     ITVKKLRESLEIVIKQLVDAIEKRKSELESSESQREQVREQIHSELAEAETCLQNALH
                     NLSLNLVDEAIQQLKKIKFDVLKQDIQLFENYEDGFRSILFDKTGIHAEKERLDKAIL
                     KTTEDIETLRNDINQLELSIHSEQEELDEVNNMITRVEKDLSRNENEKAWIDKHIQTI
                     ERQIADDVKQKVHLENEIKKLQHYISELVKEIEQWQNSMVEFNEKSNDLKQQIHDLIV
                     KRDEIEKKIIGRKQSSKKDMEQLQRLADRISSLDKNIVELQFRLNSIEEYLWTEYETR
                     LTDTSIKLDESLYESINSELQNIKQQIQELGPINNLAIEEYKDLKKRFEYYIEQKKDI
                     EKAREDILSVIADINKQSVDLFLQTFKEIQKNFQEIFKQLFNGGEAIIELTDPENVLE
                     SGIEIIVRPPGKKNKNINMLSGGERSMIAIALLFATYMVKASPFCFLDEIDAALDEEN
                     IGRFIRLVQKFSKSTQFVIVTHNKKTMSVCDSIFGVTMEEPGVSKVLSVKLETAYVMS
                     K"
BASE COUNT         1453 a          963 c          775 g         1621 t
ORIGIN      
        1 atcaagaata tattcaataa aaagatcatg gtataatcta tacaaaaagc aaacaaaact
       61 acaaagcttg tggagacggc tatcatcata aagatgttag ctatacttat catgaagatt
      121 tccctgtagt ataacgctgc catatataat aaacgggaat gcccagtagt agtacaccaa
      181 tagcaaaagc tgaggtaatg gtccagcagc agagcgctgc aatcatcatg atgatggtca
      241 atacaacata tacaagggga acaagggggt atagcggtgt tctgtacgag cttttgagct
      301 cgggatgttt tttccgaata accatgagcc ctatgacggt taacacaggg aatacattga
      361 gcgcaaaacc catgtagata accagcgaca tagccgttcc tgaatataca tagataaccg
      421 ccagaatcat ctgtaaaaca atagcataga gcggagtacc aaaacggttg ctgatttttg
      481 acaacaatga aaagatcatg ttattgttag ccatagcata atacacacgt ggccctagca
      541 tcatctgtgc agatactgac gataaaagta taatggcgat agttatacta aaaaaattgc
      601 ttgcaacagg accaaataat gcattggctg caatagcacc tatctcatca tgattcatga
      661 tattaatgcc atcggttgac ataagaaaaa caatatttaa aaggatatac aaaactgtgg
      721 tgatagttac tccccagaac attatccgcg gcaaattttt ttcaggagct actatctcac
      781 ctgcaatata ggtagcacca ttccatcctg tgtaggcaaa catcaccatt aaaagcgata
      841 gtcctatcaa tgggattgaa cctgcttcca cggtgccata gtgcgcatag agcctgtgaa
      901 tagcggaact atcgagcaca taaaaccctg caataataaa tgccgcaacg atagcaattt
      961 ttatgacagt aaggatattc tgtatggcgc ttccccgttt tacccccatt atatgaatac
     1021 agccaaatgt tatgatgata agcgcagcaa tatttttttg ccagaagata tcatcggtaa
     1081 tagtggttat accagtagca tgaatgttta taaaaaattt attgagatac tgcactaaaa
     1141 gcatggagct ggttgcaacc ggtgcagtaa atccaacaat gagtgatacc caccccgtta
     1201 aaaatgcagg gaggttgcca aatattttct ttaaataaat atattcaccg ccaacatcag
     1261 gccatagtac agctaattca ctgtagctta atgcgccaca cagagcaaca acaccaccta
     1321 acacccacaa tagtataaca atcagagcgt tgttggtcat gcccaatata ttgcctgttg
     1381 tcataaaaat cccggtgcca atcatatcgg caatgataac cattataccg gtaaagagtc
     1441 ctaactggcg tttaagctcc aaaaaattac ctcttatttt gacatgacat acgcagtttc
     1501 taatttaacc gaaagcacct ttgaaactcc gggctcttcc attgttacac caaaaataga
     1561 atcgcataca ctcattgttt ttttgttatg agtaacaata acaaactgag tggattttga
     1621 aaatttctga acaagccgta taaaacggcc tatattctct tcatcaagtg cagcgtcaat
     1681 ctcatccagg aagcaaaagg gcgaagcttt taccatatat gttgcaaaca aaagcgcaat
     1741 cgctatcatg gagcgttcgc cgcctgatag catatttata tttttattct ttttcccggg
     1801 tggacgcact ataatctcaa taccactttc aagaacattt tcaggatcgg ttaactcaat
     1861 aattgcttcg ccaccattaa aaagctgctt aaatatctcc tgaaaatttt tttgaatttc
     1921 tttaaaagtc tgtaaaaaca gatctacgga ttgtttattg atgtcagcaa taactgacag
     1981 aatatcttca cgggctttct ctatatcttt tttttgttca atataatatt caaatcgttt
     2041 ttttaagtcc ttatattctt ctattgctaa gttgttaata ggtccaagct cctggatttg
     2101 ctgttttata ttttgcagtt cgctattaat gctttcatac aatgattcat caagctttat
     2161 gctggtatca gttaggcgtg tttcatattc agtccataaa tattcttcaa ttgaatttaa
     2221 tctaaactgc aattcaacaa tatttttatc cagagagctt atcctgtcgg caagccgttg
     2281 cagttgttcc atgtcttttt ttgatgattg cttacgtcca ataatttttt tttctatttc
     2341 atcacgtttt acaatcagat catgaatctg ctgtttcaaa tcattgctct tttcattaaa
     2401 ttcaaccatg ctattctgcc attgctctat ctctttcacc agttcgctga tatagtgttg
     2461 caacttttta atttcatttt ccaggtgtac cttttgcttt acatcatcgg ctatctgcct
     2521 ttcaatagtc tgaatgtgtt tatcaatcca tgctttttca ttttcattcc tggataaatc
     2581 cttctcaaca cgggtaatca tattattaac ttcgtcaagc tcttcctgct cactgtgtat
     2641 gcttaattcc aattgattga tatcattccg taacgtttct atatcctcag tagttttaag
     2701 gattgcttta tcaagccttt ctttttcggc gtgtatacct gtcttatcaa acagtatgct
     2761 tctaaatccg tcttcataat tttcaaaaag ctgtatatcc tgcttcaata catcaaattt
     2821 aatttttttt aactgctgta tggcttcgtc aaccagattc aaagataaat tatgcaatgc
     2881 attctgcaaa catgtttctg cttcggcaag ctcgctgtga atttgctccc tcacctgctc
     2941 acgctggctt tcagatgatt ctaattcgga tttacgtttt tcaatagcat caaccagttg
     3001 tttaataact atctccaatg actccctcaa tttttttacg gttatctcaa aatccttaat
     3061 ggcatgtttt ttttgttcga tagcatctct aaatgaatgt atattctgaa ttttcttttt
     3121 ccggtgagta taaaggtttt caagctgctg tttatcgctg gcaatattgt taacgattat
     3181 ctggctttgc tgtatattct tttctttttc ttcagtcaat ttttttacag tttcatcaat
     3241 ttcattgatg cgctgtctgg tagcagtaat ttgctgttcc tgctgttgaa taagctgttt
     3301 attccgtgaa gttttcccat ctatatcttc aagttttata gtataggtat gcagcttttt
     3361 atcccattca aagagctgca actgaatatc attttttctt ttttcatcat tttcattttc
     3421 tgatgatata agggtaacct tctttgaaag ctcttcgcgt tcggcaaaaa gtttttccct
     3481 gtcttcggtt accttctgca attttttttg aaaatccctg tatcgcaata attgcaggga
     3541 aatatcattc cttttatatt tctcgcgcaa gccaaaatag atttttgttt tttcagcctg
     3601 ttttgatttt aaatcttttt cgcgttcaac ttctttaata atatcgttta accgtgctaa
     3661 attaaccgta gtgtcctcaa gcttttttaa cgactctttt ttctgaacct taaatcgcga
     3721 aatgcccgca gcctcttcaa aaatatagcg acggtcttca gcttttgttg aaagaattaa
     3781 atctattttg ccctgctcca taacagaata tgaagcttta ccaagtccgg tatcaagaaa
     3841 aagcttttca atatctttaa gcctgacagg tgatttattt atcatatatt cggattcacc
     3901 atcgcggaaa accctgcggg tgacagttac tgtgtcagaa tcaatatgta atatcctgtt
     3961 tgtgttgtta ataaccagtg aaacctcagc cagtgacaac ggtttgcgct gatcggtacc
     4021 ggcaaagata atatcttcca ttttatcgcc gcggatattc tttgcctgtt tttcgcccag
     4081 cacccatttg gttgcatcaa caatatttga tttaccacaa ccgtttggac caacgattgc
     4141 ggttatgcct ttttcgaaat gtatctttat tttatcggca aatgatttaa atccaaatat
     4201 ttccagtgag tctaaataca ttgataccac cttacaatgg ttatagcatt atgcatgatt
     4261 atagtgcagt aatttcttta cgattatcta cgggaatctt taaaaaaact ctggttaaca
     4321 tggtaaaaaa tcaggaaaaa ctgtttttac cactatagtg tcataaaaca gcattaatca
     4381 ttgccattga aaactttttc caattttctc atggtcatcg ttaaaaatca gggttttcgt
     4441 aattatcaat tattatcaat ataaacaaac atatctccaa tacgtatgcc acaaaaaaat
     4501 atccggaatt gaattatcag ggttatcctt atgccgcact ataaaaagca attacatgca
     4561 acataccaat aattgcgcaa caacattttt taaaaaaaga tttacccaga cctggctatg
     4621 ttgaggaaaa taattgttgc aataataaag taatagtatt atcatcgcag taacaggatg
     4681 atgagcacca tcaaacaaca atccttatgt ttaattggca cacataacgg cagaaactat
     4741 aaaaatagta ttgacctata tgttatgtat tatcatcaag gagttgctga tggctgcgct
     4801 gtagccgctc aa
//
LOCUS       DAOSOX010000181         4795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17794_length_4795_cov_9.009283, whole genome shotgun sequence.
ACCESSION   DAOSOX010000181 DAOSOX010000000
VERSION     DAOSOX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17794_length_4795_cov_9.009283"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(650..1864)
                     /locus_tag="PLX22_10530"
     CDS             complement(650..1864)
                     /locus_tag="PLX22_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOT20372.1"
                     /translation="MKQTIGIIGGGASGLAAAYFASQNSNNSVHIFEKQNKIGRKVAA
                     SGNGRCNLTNMNMGSEHYHGDKTFVAAVLGAFSLQDTIQFFETIGIVTTTLQNGKVYP
                     ASLQATTVVRILEHELSHNNVSIHLSRKIESIKPAASGFMVTTAGKEEHFFHKIILAC
                     GSCAYGPLGASKDGYDLARMMGHTVIEPFPAILPVTITNKPLHTLQGIRWDCTLSVII
                     DDSVVQQVTDEVLFTAYGISGPAALSIARDVNHALLQGKSVTIQCNIFPYYAYDDVRN
                     KLMYLLSQPDKSLEFALYGIMNNAMPKVFLSIAGIEYTLPAAASLNNIDAIINAFTHA
                     TLHPGAIRPFTEAVVAAGGVATDEVNPTTMQSKLVPGLFITGELLNIDGNSGGYNLQF
                     AWSTGAIAGKHC"
     gene            complement(2373..4214)
                     /locus_tag="PLX22_10535"
     CDS             complement(2373..4214)
                     /locus_tag="PLX22_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT20373.1"
                     /translation="MKDINTLFDSEVTRKDFLQSTMTLAVSLSALSLLQCSEETPQKP
                     SFNAGWITQKPESRPTMNVASSSSRVFLKNGIIIDGSGKPPFIGNVMVNNTIIEIVTP
                     KKIVFSGTTIDCTDKIIAPGFIDAHSHLDWILPMDGHPELKNPFMEQGITTVVSGNCG
                     YGVAGFLPKSKYLPMIKTVGKSFFGGDSSSENDNFVKQAAVSMITMQQYLTYCKQHGI
                     AMNMANLAGHGTTQMSLRGYDPKPLNNDEIKTMLYLLEQAMDEGAYGVSFGLQYEPGL
                     FASNDEIATIARLVKKKDKIVTCHMKAYSALSGTYPLKPFGTPHNIIALNEMIDIAKQ
                     TDVRLELSHLIFVGTKTWKTCQDALQIIDRAIDSNLDIMFDTYAYHCGTSVINVFYPN
                     WFLASTPQVYEDKMAMLKLRMQLELIVALLGFGYSDIQIINANHPELVKYNGMLLKDI
                     AKQRNMSQYDNFIDFTKKSNGRARVLNHRYSSFENIMDLMKHRASLFMTDATPYSQGA
                     QNPAAYGNYPRFFEIARDYKLLSPQQLIRKMTGAVADRYNLKKRGYLKDGYMADITIV
                     DWKKVKDNNTITQTSNRPSGIDYVFINGKKVVDKGKATGVPDAGMVL"
BASE COUNT         1203 a         1081 c         1017 g         1494 t
ORIGIN      
        1 ttttgttttc acaagagaaa aaggggcttc atgcgccagc ccccttttct cttgtgtatc
       61 tcttttcccc cggcttggcg actatcattt tttgtccctc attccattcg ggattccctc
      121 cgttccttgc acactgcggg ggctgcggaa ctcgccccga ttacatcggg tctcaaacag
      181 tcctcgccct tattccgcag tgtgcttgcg gtactcggca aaaaacgtcg ccttttgtat
      241 tggtgttgag ccacctcaaa cggcggtgag ccttattttc tgtatatagt atatcttttc
      301 catcgtgctg cggaagacat cctgcctttg tcctcgcacg agccgtatgg cgctacggca
      361 tccatgcctt cgctgttttt tcgttaatcc cgcaggtatg acttaaaaaa tggggtcaga
      421 gccgtgttaa cttcaccctt tcgttcaaag aggggctatg atattcttca ctcccttcat
      481 aatgacagcc tgcaaaagtg ccttctacaa ggtttgccgt gcaacctatt ttcatctttt
      541 ataagtagtg gcaacatgct gtttatgagg cagcttttga ctaaagtttt cataaggcag
      601 taattggctg tgtaattccc tgctacttgt gtagcctttc tgctatggtt tagcaatgtt
      661 ttccggcaat agctccagta ctccatgcaa actgaagatt atatccaccg ctgttgccgt
      721 ctatattcaa aagctctccg gtaataaaaa gtccgggtac cagctttgat tgcattgttg
      781 tgggattaac ctcgtccgta gcaacgccgc cagcagcaac cactgcctcg gtaaagggac
      841 gtattgctcc aggatgcaac gtcgcgtgtg taaaagcgtt gatgatggca tcaatgttgt
      901 tgagggatgc ggcagccggc agggtatatt caataccagc tatcgacaaa aatacctttg
      961 gcattgcatt gttcataatc ccgtataagg caaattccag cgatttatca ggttgtgaaa
     1021 gtaagtacat caatttgttg cgcacatcat cgtaagcata gtatggaaaa atattgcact
     1081 gtattgttac tgacttgccc tgaagaagag catggttgac atcccgtgca atggaaagtg
     1141 cagccggacc tgaaatgcca tacgctgtaa acagcacctc atcggttacc tgctgcacaa
     1201 cgctgtcatc aataataacg ctcagggtgc agtcccagcg tatgccctgc aatgtgtgca
     1261 gaggtttgtt ggtgatggta acgggtaaaa tagccgggaa tggctcaata acggtgtgtc
     1321 ccatcatgcg ggctaaatcg tagccgtcct ttgaggcacc cagcggtcca tacgcacagc
     1381 ttccgcatgc aagtataatt ttatgaaaaa agtgctcctc tttgccggcg gttgtcacca
     1441 taaatccgct ggcagcaggt ttaatacttt ctatttttcg ggataggtgt atggatacat
     1501 tattgtgcga tagttcatgt tcaagtattc gcaccacggt agtagcctgc agtgatgcag
     1561 ggtacacttt gccgttttga agcgtcgtgg taacgatgcc tatagtttca aagaattgta
     1621 tggtatcctg caatgaaaag gcaccgagga ccgcagcaac aaatgtttta tcgccgtgat
     1681 agtgctctga ccccatattc atattggtga ggttgcagcg gccgttaccg ctggcagcta
     1741 ctttgcgccc aatcttattt tgcttttcaa atatatgcac actgttgttg ctattctgcg
     1801 aagcaaaata tgcggcagca agccccgaag caccaccacc tataatacca attgtttgtt
     1861 tcattattct tttattacat acctgccttg cgcaatgtat caaaagcttt tttataagtt
     1921 tttgccatct cgttaagagc ttttttaact tcctggtttt tcatctcatc cattattgtt
     1981 tacaatgact ggtggaagtg ttgtgtgcct gtcccgcaac acaaagccaa tagcaccaat
     2041 agtttgtgca ggcaaagcgg ttatcaacac aatgtatact attttttcat attgatgact
     2101 caactggcta taccattata ctatcaatga cattataaaa ttttgactca tagttcaaat
     2161 aattaacgtt atatcctgaa tcagcgctca ggatgacagg tgacactaac cccccttgtg
     2221 ttcccccctt tataaaaggt gggaattgtg tggaggagtt tattggcaca aaataggttt
     2281 ttattcttca caattgtttt tgcagcttaa ttccgcaggg atgacttgaa aaatggggtc
     2341 agagccctgt caactgtagt aatggtatta ccttacagca ccatgcctgc atcaggcaca
     2401 ccggtagctt ttcctttatc aactactttt ttaccattta tgaatacata gtcaatgccc
     2461 gaggggcgat tgctggtctg ggttatggtg ttgttatcct tcaccttttt ccagtcaacg
     2521 attgtaatat cagccatata cccgtctttc aggtatccgc gttttttcaa attgtagcgg
     2581 tctgcaacgg caccggtcat tttacggata agctgctggg gcgatagtaa cttataatct
     2641 cgggctatct caaaaaatcg ggggtaattt ccataagcag caggattttg agcgccctgt
     2701 gagtacggtg ttgcatcggt cataaaaagt gatgcgcggt gtttcatgag gtccatgata
     2761 ttttcaaagc ttgaataacg atgatttaat acacgagctc ttccgtttga tttctttgta
     2821 aagtcaatga agttatcata ctggctcatg ttgcgctgct ttgcaatatc cttcaataac
     2881 atgccattgt atttaacaag ctcaggatga ttggcattta ttatctgaat gtcgctatag
     2941 ccaaagccga gcaaggcaac gattaactct aattgcatcc ttagtttcag cattgccatt
     3001 ttatcttcat atacctgcgg tgtgctggca aggaaccagt taggataaaa tacattaata
     3061 actgaagttc cacagtgata agcataggta tcaaacataa tgtcaaggtt agaatcaatt
     3121 gccctgtcaa ttatctgcaa agcatcctga catgttttcc atgtttttgt tcctacaaag
     3181 ataaggtgcg aaagctctaa tcgcacatca gtttgttttg caatatcaat catctcattg
     3241 agggcaatga tattgtgtgg ggttccaaaa ggcttcaatg ggtatgtccc tgacagggca
     3301 gagtaggctt tcatgtgaca ggtcacaatc ttgtcttttt tctttaccag cctggctatg
     3361 gttgcgatct cgtcgttgct ggcaaaaagt cccggctcat attgcaagcc aaatgatacg
     3421 ccataggcgc cttcgtccat agcctgttct aacaaataca acatggtctt tatttcatca
     3481 ttgtttagcg gtttcggatc ataccctcga agcgacattt gcgttgttcc atgtcctgct
     3541 aaatttgcca tgttcatagc tataccatgt tgcttgcagt aggtaaggta ttgctgcatg
     3601 gttatcattg aaactgctgc ctgctttaca aagttgtcat tttccgagga actatcgcca
     3661 ccaaaaaagc tttttcctac cgtttttatc atgggcaggt atttgctttt tggcaaaaag
     3721 ccagccacac cataaccaca attgccactt acgacagtgg taatgccctg ttccataaat
     3781 ggatttttta actcaggatg accatccatg ggtaaaatcc agtctaaatg cgaatgagca
     3841 tcaataaatc ccggagcaat aattttatcg gtacagtcga tggtggtgcc gctaaaaaca
     3901 attttttttg gggtcactat ctctattata gtattgttaa ccattacatt accaataaaa
     3961 ggcggcttgc cactgccgtc aatgatgatg ccatttttca agaacacacg tgaagaggaa
     4021 gatgccacgt tcatggttgg acggctttct ggtttttgcg ttatccagcc agcattgaat
     4081 gaaggctttt gtggagtttc ctctgaacat tgcagcaacg atagtgctga caatgataca
     4141 gctaaagtca tggttgattg taaaaaatct ttgcgagtaa cttcagaatc aaatagtgta
     4201 ttgatatcct tcatgatagt gccccctttt taaagaatca gtgattgagt agttgctgtt
     4261 tacacgttca caaattacag ccataacaca attgttgtaa tttgttactg agcataccct
     4321 taatgtaatt tatttcaagt atttaaaaaa tattttttat agtaccaacc ttgtggagcc
     4381 acctcaaacg gcggtgtgcc tcttttgttt tcacaagaga aaaaggggct tcatgcgcca
     4441 gccccctttt ctcttgtgta tctcttttcc cccggcttgg cgactatcat tttttgtccc
     4501 tcattccatt cgggattccc tccgttcctt gcacactgcg ggggctgcgg aactcgcccc
     4561 gattacatcg ggtctcaaac agtcctcgcc cttattccgc agtgtgcttg cggtactcgg
     4621 caaaaaacgt cgccttttgt attggtatgg atatatccgt tccacggcgg gcgcacaacg
     4681 ggatttcccg cctggcttaa tgacatggct atgtagtgtc atctacggca gtctgctcta
     4741 tcatgcgccc gcatccaaat gcgccataca tccctgtatg gctgtttatt gttgt
//
LOCUS       DAOSOX010000182         4779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17863_length_4779_cov_8.102879, whole genome shotgun sequence.
ACCESSION   DAOSOX010000182 DAOSOX010000000
VERSION     DAOSOX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17863_length_4779_cov_8.102879"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..382
                     /gene="rpmG"
                     /locus_tag="PLX22_10540"
     CDS             230..382
                     /gene="rpmG"
                     /locus_tag="PLX22_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT20374.1"
                     /translation="MREVILLSCKDCKNRNYATKKDKKKQTGKLEVMKYCRHCNKHTL
                     HKETKA"
     gene            394..579
                     /gene="secE"
                     /locus_tag="PLX22_10545"
     CDS             394..579
                     /gene="secE"
                     /locus_tag="PLX22_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT20375.1"
                     /translation="MFNKTIQFVKESRDELKKVSWPERDEVSALTMVVLITVIITSLF
                     LWLVDATLMKIVSLVMK"
     gene            582..1124
                     /gene="nusG"
                     /locus_tag="PLX22_10550"
     CDS             582..1124
                     /gene="nusG"
                     /locus_tag="PLX22_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT20376.1"
                     /translation="MIKGWYVIHTYSGHENKVKLALEKKVQNLNLADKIFQVKIPTVE
                     EHSVKDGKKVIKSKKIFPGYVLVEMIIDDETWMAIKNIPGVTNFVGSFGSENPRPLSE
                     KEVDNLFNKMGEVTTKDKVSVVMDFSVGEHVKVVDGPFKEFTGVIEEVYPEKGRLRVK
                     VEIFGRGTPVELDFVQVGKI"
     gene            1151..1585
                     /gene="rplK"
                     /locus_tag="PLX22_10555"
     CDS             1151..1585
                     /gene="rplK"
                     /locus_tag="PLX22_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT20377.1"
                     /translation="MAPKKKKIVTQIKLQIPGGQANPAPPVGPALGQHGLNIMEFCKA
                     FNDRTKDQQGTILPIIITVYEDRTYTFIVKTPPAAVLIKKALKLEKGSSEPNKKKVGT
                     ITQSQLEEIAKLKMPDLNANDVEAAKKIIAGTARSMGVEVSG"
     gene            1631..2314
                     /gene="rplA"
                     /locus_tag="PLX22_10560"
     CDS             1631..2314
                     /gene="rplA"
                     /locus_tag="PLX22_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT20378.1"
                     /translation="MKRGKKITDAKAKVDKTKLYPIDEALSLMKELAFVKFDETVEVA
                     IKVIHKSYQNVRGSVVLPHGTGKEIKVLVICKGDKQKEALEAGADFVGAEDVIEKIKN
                     GWLDFEAVVATPDMMKEVGKLGPILGKKGLMPKPKSGTVTDDVKTAVKELKGGRVEYK
                     ADKTGVIHLGVGKLSFEQQKLAENIKSFYGQVVKDKPSDAKGNYIRSFHICSTMGPGI
                     KINYKGIER"
     gene            2314..>2630
                     /gene="rplJ"
                     /locus_tag="PLX22_10565"
     CDS             2314..>2630
                     /gene="rplJ"
                     /locus_tag="PLX22_10565"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT20379.1"
                     /translation="MVKQYKIDSVNELKEKLNSKKNIILTHYSGIKVKDLYQLRKILR
                     DKGAEYKVIKNNLFRKALKDSGYPPIDTHLTGPIGVVFVDKELSEIAKVLKDFQKEQD
                     KFQ"
     assembly_gap    2631..2730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2819..3205
                     /gene="rplL"
                     /locus_tag="PLX22_10570"
     CDS             2819..3205
                     /gene="rplL"
                     /locus_tag="PLX22_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT20380.1"
                     /translation="MAKLSVQELLEAIGSLTLVEAAELVKAMEEKFGISAAAPVAVAA
                     ASGGSGAGAAEVEEKTEFDVILTGFGDNKINVIKEVRAITGLGLKEAKDLVEAGGKAV
                     KEKVSKEEAEKIKKQLVEAGATVEVK"
     gene            3482..>4779
                     /locus_tag="PLX22_10575"
     CDS             3482..>4779
                     /locus_tag="PLX22_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT20381.1"
                     /translation="MQKQQKIIRFGKIKSGIGLPNLIENQTESFKWFLQKDVPPAKRK
                     NQGLQNVFLETFPIVSPNDDIELEFVDYDLGEPKYNVQECKERDVTYAAPLKATIRLI
                     KKDTMEVREQSVYMGDIPLMTESGTFIINGAERVVVNQLHRSPGIFFFWDEAERMYNA
                     RIIPDRGSWLEFEMDHKGLIVARIDRKKKFPATIILKALGYNTNEEILKLFYATKIVT
                     LLEEKDYESLKNRRVATDIVNHETGEVFLEAGERITIDYVDILRDEKVKEVEIIEFPK
                     NKDDAYLITSLEKDDCKSSEDALLKLHQMLRPGEPTNIDNAREIFNRLFFDPRTYSLG
                     GVGRYKINKKFGFDMKEVSEDYLRKEDIVMTMKYLINLIAEVEGYIVDDIDHLGNRRI
                     RTVGELIQNQIKIGFQRMERVIKERMTIQDVDVVTPQALI"
BASE COUNT         1643 a          630 c         1058 g         1348 t
ORIGIN      
        1 gtagagcatt tgcatggtaa gcaaaaggtc accagttcga ttctggtcgt gggctatttt
       61 gttatgtatt aaaaaatttg tatttttttt aatttgagca attaattgct tgacgcaatg
      121 tgctatattt atagcgtgct ttcatatctt taaaaagcat acacattttc agtaattgtt
      181 aaacattaca taacgcaaaa aaatactatt acattaacag gatgtcatta tgcgtgaagt
      241 gatattatta tcatgtaagg attgtaaaaa tcgcaactat gccacaaaga aggataagaa
      301 aaagcaaacc ggcaagcttg aggtgatgaa atattgcagg cattgcaaca agcatacatt
      361 gcataaggaa acgaaagcat aaggtgggcg atagtgttta ataaaaccat acaatttgtt
      421 aaagaatcac gtgatgagct taaaaaagtt tcctggcctg aaagggatga ggttagtgca
      481 ttgaccatgg tggttcttat aactgttata attacttcac tttttttatg gcttgttgat
      541 gctaccttga tgaaaattgt ttccctggtg atgaaataac tatgataaaa ggttggtatg
      601 taatacatac ttattcgggt catgagaata aggttaagct ggctttagaa aagaaggtgc
      661 aaaatctgaa tttagctgat aagatatttc aggttaagat tccaactgtt gaagaacatt
      721 cagttaaaga tgggaagaag gttataaaat ccaaaaaaat atttccggga tatgttcttg
      781 ttgaaatgat tattgatgat gaaacgtgga tggcaataaa aaacattccg ggagttacaa
      841 attttgtagg ttcgtttgga agtgagaatc caaggccatt atctgaaaaa gaggttgaca
      901 acctgttcaa taaaatgggt gaggttacca ctaaagataa ggtgtcggtt gttatggatt
      961 tttcggtagg agagcacgta aaggttgttg atggtccatt taaagaattt accggtgtta
     1021 ttgaagaggt atatccagag aagggcaggt tgcgcgttaa ggttgaaata tttggcaggg
     1081 ggacgcctgt tgaattggac tttgtgcagg taggtaagat ataataatag ttatataagg
     1141 gttgatgaca atggctccaa aaaagaagaa aatagtaact cagattaaat tacagattcc
     1201 tggtggacag gcaaatcctg caccaccggt agggcctgct ctgggacagc atgggcttaa
     1261 catcatggaa ttttgcaagg cttttaacga tagaacaaag gatcagcagg gtaccatact
     1321 gccgataatt attactgtat atgaagacag aacatatacg ttcattgtta aaacaccgcc
     1381 agcagctgta ttgattaaaa aggctcttaa gttggaaaaa ggttctagtg aacctaataa
     1441 gaagaaggtt ggtaccatta cacagtctca acttgaagaa attgctaaat taaaaatgcc
     1501 ggatctcaat gctaatgatg ttgaggcagc aaagaaaatc attgctggta cggcgcgttc
     1561 aatgggagtt gaggtaagtg gataacataa ttaatataat taattaataa tgaattattt
     1621 ggggtttttt atgaagcgag gaaaaaaaat tactgatgca aaggctaagg ttgataagac
     1681 caaattatac ccgattgatg aagcattgtc attaatgaaa gaattagcct ttgtaaagtt
     1741 tgatgaaaca gttgaggttg caataaaagt tatccataaa agctatcaaa atgtacgtgg
     1801 ttccgtggta ttaccgcatg gcactggtaa agaaataaaa gtgcttgtga tttgcaaagg
     1861 tgataagcaa aaagaagctt tagaagcagg agctgatttt gtaggtgcag aagatgttat
     1921 cgagaagata aagaatgggt ggcttgattt tgaggcggtt gttgcaaccc ctgatatgat
     1981 gaaagaggta ggaaagttag gccctatatt gggtaaaaaa gggcttatgc caaaacctaa
     2041 atctggcacc gtcactgatg atgtaaaaac agccgttaaa gagcttaagg gtggaagggt
     2101 tgaatacaag gccgataaga ccggggttat ccatcttggt gttggtaaac tttcatttga
     2161 gcaacaaaag cttgcggaaa acattaaatc gttctatggc caggttgtta aagataaacc
     2221 ctctgatgca aaaggaaact atattcgttc ttttcatata tgctcaacaa tggggccagg
     2281 cattaaaatc aattacaagg ggatagaacg gtaatggtta aacaatataa aatagattct
     2341 gttaatgaat taaaagaaaa actgaattca aaaaagaata ttatattaac acattattcc
     2401 ggtatcaagg taaaagatct ttatcaattg aggaagattt tgcgggataa aggtgctgag
     2461 tataaagtaa ttaaaaataa tttattcaga aaagcattga aggatagcgg atatccccca
     2521 atagatacac atctgacagg tcctattggt gtggtgtttg ttgataagga attgagtgaa
     2581 atagcaaagg tattaaagga ttttcagaaa gagcaggata aatttcagtt nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacattatgg agtattggtt gcccttataa
     2761 gtttttacga caaatgttcg tattaacttc agattcacta agtataagga gtgacataat
     2821 ggcaaaatta tcagtacagg aattgttgga agcaattggt agtttaacgc ttgttgaagc
     2881 cgcagagctt gtgaaagcca tggaagagaa gtttggcatt tcagcagctg caccagttgc
     2941 tgttgcagct gcatctggcg ggagcggagc aggtgcagca gaagttgaag agaagactga
     3001 atttgatgtt attctcacag gttttggtga taacaagatt aacgttatca aggaagtgcg
     3061 tgccattacc ggtttaggct taaaagaagc taaagatctg gtagaagctg gaggcaaagc
     3121 ggttaaggaa aaggtttcta aagaagaagc cgaaaagatt aaaaagcagc ttgtagaagc
     3181 tggagcaaca gtagaggtta agtaacgttt attgacgcaa aaatatcttg aaaagattat
     3241 tgtaatactg tatactgaaa atagtagtag gtttggttgc gatgcaatca aacctactgg
     3301 tgtttatgtt tttgtaattt actatcaata acaataaagc gatgttctgc atttgaaaca
     3361 gtgccccaag atatatgaat aaatgatgaa tagttactat caaacaaaaa caattatatt
     3421 tttttatgtt cattgtctga tttatttata tgtatgataa attttatatc aggtgtatac
     3481 tatgcaaaaa caacaaaaaa ttatacgttt tggtaaaata aagtcaggca ttggattgcc
     3541 caacttgatc gaaaatcaaa ctgagtcgtt taaatggttt ttacaaaaag atgttccacc
     3601 tgcaaaaagg aaaaatcaag gattgcaaaa tgtatttctg gaaacattcc ctattgtcag
     3661 ccctaatgat gatatagaat tggagtttgt tgactatgat ttaggggagc ccaaatataa
     3721 tgttcaggaa tgtaaagagc gtgatgtaac ctatgctgca ccactgaaag caacgatacg
     3781 ccttataaaa aaggatacca tggaagtcag ggaacaatcc gtttatatgg gtgatatccc
     3841 cttgatgaca gagagcggaa catttatcat caatggagca gagcgtgttg ttgtgaatca
     3901 gttgcataga tcaccaggga ttttcttctt ctgggatgag gctgaacgaa tgtataatgc
     3961 acgtatcatc cccgatagag gatcgtggct ggaatttgaa atggatcata aggggcttat
     4021 tgtcgctcgc attgatagaa agaagaaatt tcctgcaacg attatattaa aagctcttgg
     4081 atacaataca aatgaagaaa tactaaagct cttctatgct acaaaaatag ttacgctttt
     4141 agaagaaaaa gattatgaat cgttgaaaaa cagacgagtt gccactgata tagtaaacca
     4201 cgagacaggt gaagtgttct tagaagctgg tgaacgtatt actattgact atgttgatat
     4261 tttacgggat gaaaaggtta aggaagttga aataattgaa tttcccaaaa acaaagatga
     4321 tgcctatctg attacctcgc tggaaaagga tgattgtaaa agttccgaag atgcactgtt
     4381 gaaattacac cagatgttaa ggcctggtga accaaccaat attgacaatg cacgggaaat
     4441 atttaacaga ttattttttg atcccagaac ctatagtctt ggtggtgttg ggcgttataa
     4501 aattaataaa aagtttggat ttgatatgaa agaggtgagt gaagattacc tcagaaagga
     4561 agatatagtc atgacaatga aatatctcat caacttaata gcagaggttg aaggatatat
     4621 tgtcgatgat attgaccatt taggaaatag aagaatacga actgtgggag agttaataca
     4681 gaatcaaata aagattgggt tccagcgtat ggaacgtgtt atcaaagagc gtatgacaat
     4741 acaggatgtt gatgttgtaa cacctcaggc gcttataag
//
LOCUS       DAOSOX010000183         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_17867_length_4778_cov_5.811560, whole genome shotgun sequence.
ACCESSION   DAOSOX010000183 DAOSOX010000000
VERSION     DAOSOX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17867_length_4778_cov_5.811560"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>1333)
                     /gene="glnS"
                     /locus_tag="PLX22_10580"
     CDS             complement(131..>1333)
                     /gene="glnS"
                     /locus_tag="PLX22_10580"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOT20382.1"
                     /translation="MKNGEFPDGSKTLRAKIDMSSPNMHMRDPVMYRIKHATHHRTGD
                     TWCIYPTYDWAHGQSDYIEGITHSICTLEFEVHRPLYDWFLDQIASGQRPRQIEFARL
                     NLSYTVMSKRMLLKLVQQGYVSGWDDPRMPTIAGLRRRGYTPDSIKIFARTVGVAKKD
                     SMVDIALLEHCIREDLNTKANRVMCVLNPLKVIITNYPENQVEYVEGINNPENQVAGT
                     RKIPFSRIIYIERDDFMENPPKKYFRLSPGSEVRLRYTYFIQCTDVIKDASGNIIELH
                     CTYDPQTKDGYAPDGRKVKGTIHWVSAHHSLPVEVRLYDRLFTREDPSQVDDGSDFTD
                     YINPDSLKTVHGFIEPSVANAKAFTNFQFERLGYFTVDPDSHDNNVIFSRTVTLKDTW
                     AKIVQKEG"
     assembly_gap    1334..1433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1434..1597)
                     /locus_tag="PLX22_10585"
     CDS             complement(<1434..1597)
                     /locus_tag="PLX22_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT20383.1"
                     /translation="MEIPPATNFIEEIINEDLKNNVCTGVITRFPPEPNGYLHIGHAK
                     SICLNFGIAI"
     gene            2372..>2631
                     /locus_tag="PLX22_10590"
     CDS             2372..>2631
                     /locus_tag="PLX22_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT20384.1"
                     /translation="MITENKQYYIGCSGWNYKHWKGLFYPETVPQYRWLQYYASIFTT
                     VEINATFYRQFKDATYTSWYNRVPNEFCYSLKLSRYITHIKRL"
     assembly_gap    2632..2731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2732..2938
                     /locus_tag="PLX22_10595"
     CDS             <2732..2938
                     /locus_tag="PLX22_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT20385.1"
                     /translation="RLHGSKKLYASEYTMRELEEWANKIIAWNKNTYCYFDNDVNAYA
                     VFNALSLKRLLHITSPLPCSEFTV"
     gene            3021..3860
                     /locus_tag="PLX22_10600"
     CDS             3021..3860
                     /locus_tag="PLX22_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT20386.1"
                     /translation="MELIDLHCHTTASDGIYKPCAIIDYAIEHGVKILAITDHDTIDG
                     LDEALCYARQKSFTLVPGIEFSIEYPHGSFHLVGMGIDHSNEELKRELSHLQKLRKDR
                     IYKIINDLQVHGIDISDKEVEQEAVGNSPGRPHVARVLVKKGYASDVKDVFVNFMVKG
                     KPGYVKKDKITFYKAIQLIKDSGGIPIVAHPISLGFGSWAEGESLIKDLFDGGIQGIE
                     VYSAMHSLEDVEQFLAIAKKYSLLISGGSDFHGDKQEVMGYYADGKPIPFEIGQQFLK
                     VLR"
     gene            3869..4681
                     /locus_tag="PLX22_10605"
     CDS             3869..4681
                     /locus_tag="PLX22_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT20387.1"
                     /translation="MKTDRGYQKSFLIDIDGVIVRGNSLISGAKDFIGNLQKGNFPFL
                     FLTNNSYYTPKELSERFFTLGLDVDESRFYTSAMATASFLVYQKPGCSAYVIGGRGLI
                     QELEKNGIVISYKNPDYVIIGETEEYDYSRIIEATYLIQEGAKFIATNPDLTGPTDRG
                     PVPACGALVAPVEKVTAVTPYFLGKPNPAMMYWARKKLNVHSANCFMIGDRMDTDILG
                     GSEAGMTCVLVLSGVTTLAMVDRFPYQPDYIVEHVGEIDPDTILSRRERIKE"
BASE COUNT         1403 a          823 c          930 g         1422 t
ORIGIN      
        1 ctcgaacctg gacttctctg atccagagtc agatgtgttg ccaattacac caccgggcaa
       61 aacaacacca taacaccttt atagcacaat gtttattctg ataaaaattt tgtcaatact
      121 tttgaaacaa tcacccttct ttttgtacta tttttgccca tgtatccttt aatgtaactg
      181 ttctgctaaa tattacattg ttatcatgtg aatcagggtc aacggtaaaa taacccaggc
      241 gttcaaactg aaaattggta aatgctttag cattagcaac cgatggttct ataaatccat
      301 gaacagtctt caatgaatca ggattaatat aatcagtaaa gtcgctgcca tcatcaacct
      361 gtgaaggatc ctctctggta aacaatctat cgtacaggcg cacttcaacc ggcagtgaat
      421 ggtgagcgct tacccagtgt atggttcctt ttaccttcct gccatcggga gcatagccat
      481 cctttgtttg tgggtcatag gtgcaatgca gttcaatgat attgccgctg gcatccttta
      541 tgacgtcagt acattgtata aaataggtgt atcgcaagcg aacttcgctt cccggtgata
      601 atctaaaata tttctttgga ggattttcca taaaatcatc gcgctcaata tagataatcc
      661 gtgaaaatgg aatttttcgt gtacctgcaa cctggttttc gggattattg atgccttcaa
      721 catattcaac ctggttttca ggatagtttg taataatgac cttcaacgga ttaagcacac
      781 acattacacg gtttgctttt gtattaaggt cttcacggat acagtgttct aaaagggcta
      841 tatcaaccat gctatctttc tttgcaacac caactgtcct tgcaaaaatt tttatagaat
      901 caggcgtata accccgccgg cgcaacccag caatggttgg catgcgggga tcatcccatc
      961 ctgaaacgta cccttgctgt actaatttta gcaacatacg cttgctcatg acagtatagc
     1021 ttaaattaag acgtgcaaac tcaatctgtc ggggacgctg accggatgct atctgatcta
     1081 aaaaccaatc atataatgga cgatgaactt caaattctaa tgtacatatt gaatgcgtaa
     1141 tgccttcaat gtaatccgat tgcccatgag cccagtcata ggtggggtat atacaccatg
     1201 tatcaccggt gcgatgatgg gttgcatgtt ttatgcggta catcaccggg tcgcgcatgt
     1261 gcatgttagg tgaagacata tctatctttg cgcgcagcgt ctttgatcca tctggaaatt
     1321 caccattttt catnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttatag
     1441 caataccaaa gttaaggcat attgattttg catgtccaat gtgcaaatag ccattaggct
     1501 ctggtgggaa gcgtgtaata acacctgtgc agacattatt tttaaggtct tcgttgataa
     1561 tctcttcaat aaaatttgtt gccggtggaa tttccatatc agtatcgcaa tgtataaaga
     1621 ttttacatac tgtaatgagc agtatatcca ttttgtcaag acattatagt gccagtattg
     1681 atataagcca ttatagtact atagcatgtg cgatagttac tggtaaggta cctgttgcct
     1741 gtgacaatgt attgttgtat tgaatatttc ctgcgtgcaa cgaagctgcc gataacagat
     1801 tttactgtca ctatgtttcg tagcttcgaa tgagaatgtc attggatacc actgtcattc
     1861 tgagggagcg ccagcgactg aagaatctat gttttaacaa aacagagaga ttcttcgctg
     1921 catttagaat gtcattggat actactgtca ttctgaaggt cgcagtcctg aagaatctat
     1981 tatttcactt ttgcaaaaag ggcaagatcc ttcgctcaga atgacagttt ttcaccatac
     2041 gccgtgcctc cgtgcacaac tatttattat cgtgataata atatacccct ctctgtattc
     2101 ccccctttat aaaaggtgga aattgtgtgg aggagtttat tagcaaaaag tacgtttgtg
     2161 ttcttcataa cagtttttgc agtttaatcc tgtaggagtg acataaaaaa tggggtcaga
     2221 gccctgataa ccccccttat tcccccattt gaacgaaagg ggggggaatt gtatggaatt
     2281 ttgttgaata atccatgaca ttgtatacac tatttttgct ttatagatta tagtatcaat
     2341 agtttgatgt tttttaaagg gcaattgcgc aatgataaca gagaacaaac aatattacat
     2401 cggttgctcg gggtggaact ataagcactg gaagggattg ttttatcctg aaactgttcc
     2461 acaatacagg tggctacaat attatgcatc catctttacc actgttgaga tcaatgccac
     2521 cttctatcgg caatttaagg atgcaaccta taccagctgg tataacaggg tgcccaatga
     2581 attttgctat agccttaaat tgtcgcgata tattactcat ataaaaaggc tnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgtttgcat ggttcaaaaa aattatatgc
     2761 atcagaatac accatgcggg agcttgaaga gtgggcaaac aagattattg catggaataa
     2821 aaatacctat tgttattttg ataatgatgt caatgcgtat gcggtgttta atgctctatc
     2881 tcttaaacgg ttattgcata taacatctcc gctgccatgc tctgagttta cagtgtaact
     2941 atttgtgata aacaataatg attgcaattt ttttcttctg tgctattata taccattaca
     3001 cctataaaag gatatgatga atggagctta ttgatctgca ttgtcacact actgcaagcg
     3061 atggcatata taaaccctgt gcaattattg attatgctat tgagcatggt gtcaaaatcc
     3121 ttgcaataac tgatcatgat accattgatg gacttgatga ggcgctttgt tatgcacgtc
     3181 aaaaatcgtt tacattagta ccagggatcg aattcagcat tgagtatcct catggatcat
     3241 ttcatcttgt tgggatgggg attgatcata gcaatgaaga gttaaaaagg gaactgtcgc
     3301 acctgcaaaa gttacgaaaa gacaggatat acaaaataat aaacgactta caggtacatg
     3361 ggatagatat ttctgataaa gaggtggaac aggaagccgt tggcaactca ccgggacgac
     3421 cgcatgtggc acgtgtactg gtaaagaaag gatacgcttc agatgtaaaa gatgtttttg
     3481 ttaatttcat ggtgaaagga aagcctggtt atgttaaaaa agataagata acattttata
     3541 aagcaataca acttataaaa gatagtggtg gcatccctat tgttgcgcat cccatttcgt
     3601 taggttttgg ttcatgggct gaaggcgaaa gcctgataaa agatttattt gatgggggta
     3661 ttcaggggat agaggtatat tcagcaatgc atagccttga ggatgtggag caatttttag
     3721 caattgcaaa aaaatattct ttgcttattt caggtggcag tgatttccat ggcgataaac
     3781 aggaagtaat gggatactat gcagatggta agcccatacc atttgaaatt ggtcagcaat
     3841 ttttaaaggt attacgttaa ggtactatat gaagacagat agaggttatc aaaagtcgtt
     3901 tttaattgat attgacggtg ttattgtacg aggtaattcc ttaatttcag gagcaaaaga
     3961 ttttattggc aatcttcaaa aaggtaattt tccgttttta ttcctcacca ataattcata
     4021 ttatacacct aaagagttat ctgaaagatt ttttacattg ggtcttgatg ttgatgaatc
     4081 acgattctat acctcagcta tggctacagc aagctttctg gtatatcaaa agcctggctg
     4141 ctcagcatat gttattggtg gcagaggatt aatacaggaa ttggaaaaaa atggtattgt
     4201 catttcgtat aaaaaccctg attatgttat tattggcgaa acagaagaat atgattattc
     4261 ccgtattatt gaagcaacct atcttattca ggaaggagca aagtttattg caacaaatcc
     4321 tgatttaacc ggaccaactg acaggggccc tgtaccagca tgcggagcac ttgttgcacc
     4381 agtagaaaaa gtaaccgctg taacgccata ctttttaggg aagcctaatc ctgcaatgat
     4441 gtactgggca cgtaaaaagt taaatgttca ctcagcaaac tgtttcatga ttggagatag
     4501 aatggatact gatattttag gcggatctga ggctggtatg acatgtgtgc ttgtgctgtc
     4561 gggtgttaca acactggcaa tggtagatag gttcccatac cagccggact atattgtaga
     4621 gcatgttggt gaaatagacc ccgatacaat actttcacgc agggaaagaa ttaaagaatg
     4681 atagtatcat aaaatgatga atatatataa acatatagat atataaatat atataaacaa
     4741 atatagtgat aattacataa aataacagac aacataga
//
LOCUS       DAOSOX010000184         4649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_18508_length_4649_cov_8.142360, whole genome shotgun sequence.
ACCESSION   DAOSOX010000184 DAOSOX010000000
VERSION     DAOSOX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18508_length_4649_cov_8.142360"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            841..3009
                     /locus_tag="PLX22_10610"
     CDS             841..3009
                     /locus_tag="PLX22_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOT20388.1"
                     /translation="MKRFVKNIASEREYVVSREKDVPVSQYYGEDTFNFEVMRQKLPA
                     DVCKKLMNCIKEADKLDTEIAQTVAHAMKEWAIDKGATHFCHWFQPMTGTTAEKHDSF
                     IELTEEGIPIERFSASQLVKGEPDASSFPSGGLRSTFEARGYTAWDPSSPAFILRNGI
                     GATLCIPTVFLSYTGEALDNKTPLLRSINVLNKSALNMMRLLGNDRATRILATLGPEQ
                     EYFLIDMDYYYKRPDLVLTGRTLIGAPPSKGQQLEDHYFGSIKERILSYMHDVEEELY
                     KLGIPAKTRHNEVAPAQYEIAPIFEEANIAVDHNHLIMETLKRVAQKHKLAALLHEKP
                     FAGINGSGKHCNWSLSDNFGNNLLNPGQSPQDNIQFLVFLTATIRAVYYHADLLRASI
                     ASSSNDHRLGANEAPPAIISVFLGEQLTKILYDIEQGVITKATNKAIIDMGLSTLPVL
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSIALPITILNTIVAESLDILADKIRTKSG
                     DVRQAAIDVIREEFKTVKPILFMGDNYSKEWEKEAAKRGLPNKKVTAEALEDLVTDKA
                     KELFNKYGILSTVELEARYTIRLERYIKEVDIEAKTLLDMVQTKVIPAALRYQNSIAS
                     SCLRIKDLLGDGAPLDAQKQLCETIAVLSNDIIKEANKLAGLIEKVTVMDDEIEQAKL
                     YAYDVKLQMEALRSKVDLIETVASDNLWPFPRLWEMLFIS"
     gene            complement(3006..4313)
                     /locus_tag="PLX22_10615"
     CDS             complement(3006..4313)
                     /locus_tag="PLX22_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOT20389.1"
                     /translation="MKAQSSLGVVFFPAFDWAIHPLHPEREERLLYTLDQLNEEGIFD
                     VEGITQYKPGLATQEDIMRTHFCFPDVSSVTTESHLISAGGTIKAAALVLDKIVKKSF
                     ALVRPPGHHAMKVVHGARGFCNINIEAVMVEYIRENYPVKKIAIVDTDCHHGDGTQDI
                     YWHDKDILYISLHQDGRTLYPGTGAIHELGGPNALGTTINIPLPPNTSDEGFLMAMER
                     VVLPVLKDFNPDIIINSAGQDNHYSDPITNMNFSASGYAQLTDMLNADIAVLEGGYSI
                     QGALPYVNLGITLAMAGLDYSYVHEPDYAIKDLTQDREITKYIKKLCDKVLQRYEECK
                     KPSFKLMPGNYDVRKKEIYYDTDNIHEMQLESIMVCDQCRGVLKIETTSSINPLCLGI
                     EIPSGACDQCISEGYRLLDHALSGNRYKYVQLIDRINKKYVMY"
     gene            complement(4310..>4649)
                     /locus_tag="PLX22_10620"
     CDS             complement(4310..>4649)
                     /locus_tag="PLX22_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOT20390.1"
                     /translation="VANAIGAAVARTTVEIELFADTQRCRLLIPNLEVNTRIPAGYTL
                     DNAIDDAKNYMIQYLTTLGVDEKEREIEIVDAYSFNMVKGFETVGKNIRVRCQVKPGI
                     LHQCKDILKI"
BASE COUNT         1418 a          874 c          982 g         1375 t
ORIGIN      
        1 ttaccttgat actgttaagc agtgcggcat tataaccgaa cttatagaaa caaggttata
       61 taaaataccg gttccgcaaa acaggctgtt tatggaaatt ggatgcttaa ccggtgatgt
      121 gggaaaggtc tatggataaa tcaaaaatac tttaatggtg gcgatagtta ttgtttatca
      181 atttcccgtt atatataaaa ctataagcgc aatgtattcc cgtatagcct ttgatgaatt
      241 gcacaggctt gaaaattttg gcatgaagct gtaaacagta tagtgcaaat ccagcttgga
      301 atcggtgggt acaggttcaa catgcagtga tgaggcatga aacagtggat aacccgtaga
      361 gcaaaaaagc cgaaacaaat gcagtaattg aagctattcg ccgctttgat agtaaaccgc
      421 tgataatgaa taacactata aatatccctg gcggaatgat aaaataggta atgcattttt
      481 taataaagaa catgtcttcc tcgcatataa attaactttg ttgctatact attcataaat
      541 tgtcagggaa aaatggcatg atgcataatc ccctcggtgg tttttagcta tgatacacct
      601 tatatcaatt gatagcatga tgattacaag taaaaaatta tggttgaatt tttttcaggc
      661 aattgaataa tcaaaggaac tatcgcccat acattattat ttaccattta catgttacag
      721 aaatgttaaa tatgcagcat ttatggctgt attgggaggt aagctcttga cttttattgt
      781 gaataatttt gtgttgctat acattgtagc tgttacacta acattataac gaggtatacc
      841 atgaaacggt ttgttaagaa tattgcatct gagcgtgagt atgtagtgtc acgggaaaag
      901 gatgtgccgg tgtctcaata ctatggtgag gatacattta attttgaagt gatgcggcaa
      961 aaattaccgg cagatgtttg taaaaagctg atgaactgta taaaagaagc tgataagctt
     1021 gatacagaga tagcacagac ggttgcgcat gcaatgaagg aatgggcaat agataaaggt
     1081 gctacccact tttgtcattg gtttcagcca atgacgggga caactgctga aaaacatgac
     1141 tcatttattg aattgaccga ggaaggaatt cctattgaac ggttttcagc aagccagctt
     1201 gttaaaggtg aaccggatgc ttcaagtttc cccagtggag gcttgcgttc aacctttgag
     1261 gcgcgcggat atactgcatg ggatccatca agccctgcat ttattttgcg caatggaatt
     1321 ggtgcaaccc tgtgtattcc aacagttttt ttgtcatata ccggtgaggc tcttgacaat
     1381 aaaacaccat tgctgcgttc aataaatgtt ttaaataaaa gtgctttgaa catgatgcgc
     1441 ctgctgggta acgatagagc cacaagaatc ttagcaaccc ttggtcctga gcaggagtat
     1501 tttcttatag atatggatta ctattacaaa cgtcctgacc ttgtgttaac cgggcgtacc
     1561 cttattggcg ctccaccgtc aaaaggacag caactggagg atcattattt tgggagcatt
     1621 aaggagcgga tactttcata tatgcatgat gtggaagaag aattatataa attggggatt
     1681 cctgcaaaaa cacgtcacaa tgaagttgct cccgcccagt atgaaatagc tccaatattt
     1741 gaagaagcta atattgctgt tgaccataac catttaatta tggaaacatt aaagcgtgtt
     1801 gcccaaaaac ataagcttgc ggcacttctt catgaaaaac cttttgcagg tatcaatggt
     1861 tcagggaagc attgcaactg gtcattatca gataattttg gtaataacct gcttaatccc
     1921 ggtcaatcac cgcaggataa tattcagttt ttagtttttt taacggcaac catacgagca
     1981 gtgtactatc atgctgattt actgagagca tctatcgctt ccagcagcaa tgatcaccga
     2041 ttaggtgcaa atgaggcacc gcctgcaatt atctcagtat ttttaggcga gcagctaaca
     2101 aagattttat atgacataga acagggtgtt ataacaaagg ctaccaataa ggctattatt
     2161 gacatggggc tttcaacact gcctgtgcta agtaaggata ataccgaccg caaccgcacc
     2221 tcgccttttg cttttaccgg caataagttt gaatttaggg ctgtaggctc ctcgcaatcc
     2281 atagcattac cgattacaat actcaacact attgtagctg aaagccttga tatacttgcc
     2341 gataaaatta gaacaaagag cggcgatgta aggcaggcag ccattgatgt tatacgggaa
     2401 gagtttaaaa cagtgaaacc tatccttttt atgggtgata attattcaaa agaatgggag
     2461 aaggaagcag caaaacgtgg tttgcccaat aagaaggtta ctgccgaggc tctggaggat
     2521 ttagttaccg ataaagctaa agaacttttt aacaaatacg gcatactttc aacggttgag
     2581 ctggaagcgc gctatactat caggctggaa cggtatataa aggaagttga tatagaagca
     2641 aaaacacttc ttgatatggt acagacaaag gtaataccag cagcattgcg ttatcaaaat
     2701 tcaatagctt catcgtgttt acggatcaaa gatcttctgg gcgacggagc accgctagat
     2761 gcacaaaagc aattgtgtga aacgattgct gtattaagca atgatattat caaagaagct
     2821 aataagcttg ccggattaat tgagaaggta acggtaatgg atgatgagat agagcaggca
     2881 aaactgtatg cgtatgatgt taaattgcag atggaagctt tgcgaagtaa agtagatttg
     2941 atagagacag tagcaagtga taacctgtgg ccatttccaa gactgtggga gatgctgttt
     3001 atttcttaat acataacata tttcttgttt attctgtcaa tgagttgaac atatttatat
     3061 ctattgcctg acagcgcatg gtcaagcagg cgatagcctt cgctaataca ttggtcacat
     3121 gcgcctgagg gaatttcaat gcccaggcac aaggggttta tcgatgaagt ggtttcaatc
     3181 ttcaatactc cgcggcattg atcacatacc atgatagatt caagttgcat ctcgtggata
     3241 ttgtccgtat catagtagat ctctttttta cggacatcat aatttcctgg cataagctta
     3301 aatgaaggct tcttgcactc ttcgtatcgc tgaagaacct tatcgcacag ttttttaata
     3361 tattttgtta tttcacgatc ctgagttaga tctttaattg cgtaatcagg ctcgtgcaca
     3421 tatgaataat caagacctgc catagccagt gtaataccta aatttacata gggcagtgca
     3481 ccctgaatag agtaacctcc ttcaagtact gctatgtcag cattgagcat atccgttaat
     3541 tgtgcatatc cggatgctga aaaattcata ttggttattg ggtcagagta atgattatcc
     3601 tgcccggctg agttaatgat aatgtcggga ttgaagtctt tcaatacagg taatactacc
     3661 cgctccattg ccattaaaaa accctcatcg gatgtattgg gtggcagtgg aatgtttatg
     3721 gttgttccca gtgcgttggg accgcctaat tcgtggatgg caccggtacc ggggtatagc
     3781 gttcttccgt cttgatgtaa tgagatatac agtatatcct tatcatgcca gtaaatatcc
     3841 tgtgttccat caccatgatg acaatcagta tcaactatag caattttttt tactggatag
     3901 ttttcacgga tatattcaac cataaccgct tcaatgttga tattacaaaa accgcgtgct
     3961 ccgtgtacca ctttcattgc atgatgaccg ggtgggcgca ccaatgcaaa cgatttttta
     4021 acaatcttat ccagcaccag cgctgctgct tttatggtac cacctgctga tatgagatgc
     4081 gactcggtgg ttacactgga tacatcggga aaacaaaagt gtgtacgcat aatatcttct
     4141 tgtgttgcaa gacctggttt atactgtgtg ataccctcaa catcaaatat accttcttca
     4201 ttaagctgat ccagtgtata cagtaatcgc tcctcacgtt ctggatgaag aggatgtatt
     4261 gcccagtcaa aagccgggaa aaatacaaca cctaatgatg attgtgcttt catatcttca
     4321 atatgtcctt acattgatgc aatatgccgg gtttaacctg acaccgtaca cggatatttt
     4381 tccctacagt ttcaaagccc tttaccatat taaaggaata tgcatcaact atttctattt
     4441 cccgctcttt ttcatcaact cccagtgttg ttaaatactg tatcatatag tttttagcat
     4501 cgtcaatagc attatccagg gtatagccag ccgggatacg ggtattaacc tctaaattag
     4561 ggataagaag gcggcaacgc tgtgtgtctg caaaaagctc aatctcaaca gtagtgcggg
     4621 caactgctgc accaatagca ttagctacc
//
LOCUS       DAOSOX010000185         4640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_18572_length_4640_cov_8.521919, whole genome shotgun sequence.
ACCESSION   DAOSOX010000185 DAOSOX010000000
VERSION     DAOSOX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18572_length_4640_cov_8.521919"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLX22_10625"
     CDS             complement(<1..211)
                     /locus_tag="PLX22_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20391.1"
                     /translation="MVNNKYKIFLLLMLNLISVGLLNYLAYAQVDNSRIAGMIIKDEK
                     LLVPGMGGGGVLLGSTEKDVMMTRAK"
     gene            complement(216..1025)
                     /gene="rsmG"
                     /locus_tag="PLX22_10630"
     CDS             complement(216..1025)
                     /gene="rsmG"
                     /locus_tag="PLX22_10630"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT20392.1"
                     /translation="MMNAGDNIDCNVINETLWEYASLIYDYNKITNITGFHTVEAIYK
                     ELIIKSITPLQNLNVPRGTSFVDIGTGAGIPGLALLLYFHGMVNGLLIDSNNKKIQFV
                     KEAVKKFDLHNYCTIIHGRAEDLAHDIHFREKFDFVVSRAFAHPYMAIEYGLPFVKVG
                     GFYYIYYQVDNFEQSIDEIKSTIGDRLYGEYRHGNGADKNNNTSLARDENYQMKNVLQ
                     HAEECGGHLASQEELMKRGLTGGLVVIKDKTMPLCFPRKNAVVKRIIHKLR"
     gene            complement(1987..3876)
                     /gene="mnmG"
                     /locus_tag="PLX22_10635"
     CDS             complement(1987..3876)
                     /gene="mnmG"
                     /locus_tag="PLX22_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT20393.1"
                     /translation="MIRYFDIVVVGAGHAGAEAALASARRGFSTLLVTGNLDTICQMS
                     CNPAIGGLAKGHLVKEIDALGGEMALAIDEAGIHYKMLNKSKGPAVWAPRAQADKKHY
                     QLRMKEVLEKQKNLTIVQDIVEKLMVEGNQIKGITTIRGQHHASQAVILCTGTFLKGL
                     IHIGNYNEKAGRLGDFSAEMLSDSLKALGFPVKRLKTGTPPRVNGNTINFNKCEMQQP
                     DPVPTPFSYRTKSIDRLQVPCYITHTTEKTHAIIVNNLHLSPLYSGKIKGIGPRYCPS
                     IEDKVVRFSSRNQHHLFLEPEGLHTNEYYINGFSSSLPEDVQLEMIKTIPGLEEVQVM
                     RPAYAVEYDYVPPIELYHTLETKRVSGLYHAGQINGTSGYEEAAAQGIVAAINAGNKI
                     SGKSPLVLQRSEAYIGVLIDDLVTKGADEPYRMFTSRAEYRLLLRQDNADERIMKYGY
                     EAGLIDKELYSDTLKKYKEINELIQKMSALSIVVDEKIKIILSKKAIPIGEKASFNAG
                     KLLKRPEITIDDILPIINEQDSSFTMTYDEAKIVEMSIKYDGYIKKDLERIKKLKMME
                     NKKIPATVDYSTIQGLKNEAREKLKTVRPETIGQAMRISGVDPSDISIVLVHIESLNK
                     NKTGA"
     gene            4021..4560
                     /locus_tag="PLX22_10640"
     CDS             4021..4560
                     /locus_tag="PLX22_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOT20394.1"
                     /translation="MNNYDTTILERIHIIQGDITKMDVDAIVNAANPSLLGGGGVDGA
                     IHRAAGPQLLEECRTLGGAKPGEAKITKGYRLPAKYVIHTPGPVYRGGNQHESTILKN
                     SYHNSMQLAKQYNCRSIAFPAISTGVYGYPKDEACRIAIDTVLDFMQKENFLIDVYFV
                     LFDDENFSLYINYLKELSK"
BASE COUNT         1368 a          947 c          766 g         1559 t
ORIGIN      
        1 actttgccct ggtcatcata acatcttttt ctgttgaacc aagcagaaca cccccgcctc
       61 ccataccagg aactaataat ttttcatctt ttattatcat ccccgcaatc ctgctattat
      121 caacctgagc ataagcaaga taatttaata aaccaacact gattaaatta agcattaata
      181 ataaaaatat cttatattta ttattaacca tatagctacc gtaatttatg aattattctt
      241 ttcacaactg cattctttct tggaaaacat aatggcattg ttttatcttt tataacaacc
      301 aatccccctg tcaaccccct cttcatcaat tcttcttgag atgccagatg acccccacat
      361 tcttcggcat gttgcaaaac atttttcatc tgatagtttt catctcttgc caaactcgta
      421 ttattgtttt tatctgcacc attgccatgc ctgtattcac cgtacaatct atccccaata
      481 gtactcttta tttcatctat actttgctca aaattatcca cttgataata tatataataa
      541 aatccaccaa ctttgacaaa tggtaacccg tattcaattg ccatatatgg atgcgcaaaa
      601 gccctgctta caacaaagtc aaatttttca cggaaatgaa tatcatgagc caggtcttca
      661 gcacgcccat gaattatggt acaataattg tgcaaatcaa attttttcac agcttccttt
      721 acaaattgta tttttttatt attggaatct atcaacaatc cattgaccat cccatggaag
      781 tatagcaata atgccaaccc ggggattccc gctccggttc ctatatcaac gaaggatgtt
      841 ccacgtggaa catttaaatt ttgtaaaggt gttatgctct taataataag ttctttataa
      901 atagcttcaa cagtatgaaa tccagttata tttgttattt tattatagtc ataaattaaa
      961 ctggcatatt cccataatgt ttcatttata acattacaat caatattatc acctgcattc
     1021 atcatatata ccaacttttg cacttttaat acaactattt catcgcacga aagagatagt
     1081 tgccagtgta ctgtttaata cttgtaatta ctcagctaaa aattttcaat agaaattttt
     1141 ttactattat gttttaggca tatagtacat gatgcctcat gaaccgggga gatgctgaaa
     1201 agatatatgc cccgtgtaaa gatgcagttt caatcttatg cttcagttag ttggcagaaa
     1261 agagaagcct ggagttcagc tcacgaagaa tagccacaac atcagcaagc cctcttcaag
     1321 tgataggcaa caagtatttt aaattcctaa aatatctatc catatgacag agtataaggc
     1381 agagataaag gattgccatc atggcggagt tgataataaa gttgattttc ctgaagactt
     1441 caaacgtaat gccccgtatg tgaattacct gagatgagcg acaccatatt tcaggaatta
     1501 tcagttgatg cctctggttc ggtctatgga aattttttaa gatatatttt ttatcccaat
     1561 tgcttggagg gattttgtta atatcgcaaa gtaatgtggc aggtcattaa tgacctttta
     1621 tgacatggtg attgataagt tgatacattc aaaagttatc aattttggcg aaaccgcgta
     1681 aatacaggct gaaatctaca ttggtttcat aacgatagcc tgcctctttt gacatcgtat
     1741 ttttggcatc ataaaatgga gtctctcctg tgggtagtca tctgcaatgg cccggatata
     1801 tattcccata acattcaggc tggctttgga tgggcatcct tatatcatcc gtaatgtttt
     1861 atgaaaatat attataatgc tatacccatt atattgccaa tgtgacataa tgttccacgt
     1921 ggaacatacc gattgttgaa gtattcacat ataatatttc tatacaatac attgttatca
     1981 atatacttat gcgcctgttt tatttttatt caaagactct atgtgaacca atacaattga
     2041 aatatcagat gggtccactc cagatattct catggcctgc cctattgttt caggccttac
     2101 tgttttcagc ttttctcgag cttcattttt tagcccctgt atggtactat aatcaactgt
     2161 tgcaggtatc tttttattct ccatcatttt caattttttt attcgttcaa ggtctttttt
     2221 gatataccca tcgtacttaa tactcatttc aacaatcttt gcctcatcgt atgtcatggt
     2281 aaatgaacta tcctgttcat tgataatagg caaaatatca tcgattgtga tctcaggcct
     2341 ttttaacagc ttccctgcat tgaaagatgc tttttcaccg attggaattg cttttttgct
     2401 tagaataatc tttatttttt catcaacaac tatcgacaat gcagacatct tttgaataag
     2461 ctcgtttatc tctttgtatt tttttaaggt atcagaatat aattctttat cgataagtcc
     2521 agcttcatag ccatatttca tgatacgttc atcagcatta tcctgccgca ataacaatcg
     2581 atattctgca cgtgaggtaa acatccggta tggttcatct gcccctttgg taaccagatc
     2641 atctatcaaa acaccgatgt acgcctctga acgttgtaat accagggggg atttacctga
     2701 aattttgttg ccagcattta ttgcagcaac tattccctgc gctgcggctt cttcatatcc
     2761 tgacgtccca tttatctgac ccgcatgata taatcctgat accctttttg tttccaaagt
     2821 atggtataat tcaatgggag gtacatagtc atattctact gcatatgcag ggcgcataac
     2881 ctgtacctct tctaatccag gtattgtttt aatcatctcc agctgtacat cctctggcaa
     2941 tgaacttgaa aatccattaa tgtaatattc attggtatgt aaaccctcgg gctctaaaaa
     3001 tagatgatgc tggttccgtg atgaaaatcg tactacctta tcctcaattg aaggacaata
     3061 acgcggtcca atacctttaa tttttcccga atagagtgga gagagatgta aattattaac
     3121 gataatggca tgggtctttt cggttgtatg ggttatataa cagggaacct gtagtctatc
     3181 tattgatttt gtacggtatg aaaaaggtgt tggtacagga tcaggctgct gcatttcgca
     3241 cttgttaaaa ttgatggtat taccgtttac acgaggaggt gtcccggtct taagacgttt
     3301 tacaggaaaa cccaatgctt ttaatgaatc cgatagcatc tctgcagaaa aatcccccaa
     3361 cctcccggct ttttcattgt aattgccgat atggatcagc ccctttaaga atgtcccggt
     3421 acacaggata actgcctggg aagcatggtg ttgtcctcgt attgttgtaa ttcctttaat
     3481 ttgatttcct tcaaccatta gcttttctac aatatcctga acaattgtta aatttttctg
     3541 cttttccaat acctctttca tacgcaactg atagtgtttt ttatcagcct gcgctcgcgg
     3601 agcccataca gcagggccct tgcttttgtt gagcatctta taatgtattc cggcctcgtc
     3661 tatcgccagt gccatctcac cacctaatgc atcaatctct tttaccagat gtccttttgc
     3721 caatccgccg atagccgggt tacagctcat ctggcagata gtatctaaat taccggtaac
     3781 caatagtgtt gaaaaaccac gccgtgctga agctaaagct gcttcagcac cagcatggcc
     3841 cgcaccgaca actactatat caaaatatcg tatcattgta tggtaactat cgcccttact
     3901 tttaattttc caataatatg cttgcaattg ctacatattt ttgtatacta taaatataat
     3961 atcgtaaaat gggtgattac aaatattttt taatcgttta cacaaatgat tggagttatt
     4021 atgaataact atgatacaac catactggaa cgaatccata tcatacaggg tgatataacc
     4081 aaaatggatg ttgatgccat agtcaatgct gcaaatccat ctttacttgg tggaggtggg
     4141 gttgatggag ctattcatcg tgcagccggg ccacaactat tagaagaatg ccgaacgctg
     4201 ggtggcgcca aacctggtga agctaagatt acaaaaggct accgacttcc tgcaaaatac
     4261 gttatccata ctcccggacc tgtgtatcga gggggcaacc agcatgaatc tacaatactc
     4321 aaaaattcat atcacaacag catgcaactg gcaaaacaat ataattgtag atctattgcc
     4381 ttccctgcca tctctactgg tgtctatggt tatccaaagg atgaagcgtg caggatagcc
     4441 attgatacag ttcttgattt tatgcaaaaa gaaaatttct taatagatgt atattttgtg
     4501 ctttttgatg acgaaaattt ttctctatat atcaactatc ttaaggaact atcgaaatga
     4561 attattgtga aagtttgcag tggtatacac agaaatataa cagtatagta tgcatgggtc
     4621 ttgatccagt gattgaagat
//
LOCUS       DAOSOX010000186         4614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_18706_length_4614_cov_8.755868, whole genome shotgun sequence.
ACCESSION   DAOSOX010000186 DAOSOX010000000
VERSION     DAOSOX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18706_length_4614_cov_8.755868"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1219
                     /locus_tag="PLX22_10645"
     CDS             242..1219
                     /locus_tag="PLX22_10645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT20395.1"
                     /translation="MIQQPLSPHEQKLISIVPDGYAPMRLDVYLAHRFTYLSRTKWQH
                     EIKTGKIVVNEKQVTNPHYIVYQHYIIEYKRPSVQEPAIIPHYTILYEDDYIIAVNKP
                     GNLPTHPAGKYFHNTLTMLLWNDIGIQCMPLNRLDRETSGVAVLAKSGSIASAFLKYS
                     HTIAKQYYAIVTGNCSFSEITVAMPIGLSPTSSIRKKRSAYTDAHWQAQTHIKVLQHY
                     DTCTLIEATPKTGRTHQIRVHCEHIGHPIIGDTLYCNSDTTFIEYIKNKGALLREFQR
                     CALHAHIYKFYHPFLQKMIAIKAPMPHDMHTFLLRLDKNYGTTNNTTKS"
     gene            1188..1709
                     /locus_tag="PLX22_10650"
     CDS             1188..1709
                     /locus_tag="PLX22_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOT20396.1"
                     /translation="MAQPTIPPKAKLFIGLLTSHDEVLFHVINILKKKFGVIDLQSEP
                     IPFSHTDYYSTIGKNLYKVLIGFNRLIKREEIAKIKLFTNKVENKFLKNGNRTINIDP
                     GYLTLSNVFLVSCKDYFHRIYIGRGIYAENEYRYVAKHYHPWEWTYPDYRKKEYLQFF
                     ETLRSIYYEQIKK"
     gene            complement(1722..2741)
                     /locus_tag="PLX22_10655"
     CDS             complement(1722..2741)
                     /locus_tag="PLX22_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20397.1"
                     /translation="MKKLIIIVFLLLLIQSQEFASSRRDTALLYHHNEGEHCIDYIAM
                     YDLLATINTLYLNGYYGLMEKHINNFMKDFPPTKEVFYFKAITLINKKKYDDGLKELF
                     IALAMEPKFSRAQNAAGYIHALRSQWATALQFFREANSNNTYNAFIEYNIAGVYYQLL
                     DFSNALNWSKKVVEHKPNFVQGYYLCALSAMQLNDFASAIEYFNIVIQLKMKNDSMYY
                     NRALAHYFLGNYEKALNDCTTALTINKKNYFAAMLTGIINVNQKNYEKALKVFTDVLK
                     IKKDDLFAKVFQALCIHTINKNISNQLFFAIFGSIDNGEQVAAQLTQLYQLKPVEPLP
                     QFFLY"
     gene            complement(2807..3964)
                     /locus_tag="PLX22_10660"
     CDS             complement(2807..3964)
                     /locus_tag="PLX22_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOT20398.1"
                     /translation="MRFLKFLVAIVMVAGVAAPVFAADSIAKLTLGDDQFIELHYLLQ
                     VQAYSLQKEGATATGDEDYWSKDTKLRRSRIIVKGQAAQNVEFFMETDAPNQEGNESK
                     NLYTQDAYIDFTIAKELKIAFGHILLPFMHHDRQSAATLLCADYTAAVVPVGSNVWRD
                     TGVELRGLLFNGLVDYRVGAWDGQDRKLDTTTNTVVNEDDMPRVTGRIQINLKDAEDG
                     FFYSGNYLGKKSIISFGAGLDYQPDAYDDGSDAKNYYAWTVDAYFDHPIGTNNALTLQ
                     AAYVYVKNSPFFDIDPVAAGTQGADKAKMFFAEGGFLINNTWQPVARFYYKDTESLAG
                     DKNKVSEIAGGLNYYLKGHSANVKVEYAQELKDEADDYKNKRITVQCQIFI"
     gene            4308..>4614
                     /locus_tag="PLX22_10665"
     CDS             4308..>4614
                     /locus_tag="PLX22_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20399.1"
                     /translation="MRFAYHFKKWHLFYLFLILIAAYYIVATHPLGTYNAYPDKLSPK
                     EKESSLSTLPLDTRSPCPKFRANGLQNGRTTVQSKASMVQPWEFKTGKGIFSSPVVDE
                     "
BASE COUNT         1486 a          968 c          745 g         1415 t
ORIGIN      
        1 ataataccga taactatcga tgtggaggct atacattcag gtttttctta gaagacggga
       61 tacaatgttg ccaattttac tggggaccac cgccggaaat actaaaacga gcactccaaa
      121 catcatgaaa catcaaaatc ttctctaata ctatcgcaca actttattaa atactttatg
      181 aacattttaa agtacagtaa aatagtttac aaaacatgag aatattttca ctataataat
      241 tatgattcaa caaccattat caccccatga acaaaagctc atatccatag tacccgatgg
      301 ttatgctcct atgcgtcttg atgtatattt agcacatcgg ttcacctatc ttagccgtac
      361 aaaatggcaa catgagatca aaaccgggaa gatagtggtt aatgaaaaac aggtaaccaa
      421 cccgcactat attgtttatc aacattacat aattgagtat aagcgcccgt cagttcagga
      481 acctgcaata ataccccatt acacaatact ctatgaagat gattacatca ttgctgttaa
      541 taaaccgggc aatcttccca cacatccagc agggaaatac tttcacaata ccctcacaat
      601 gttgttgtgg aacgacatcg gtatccaatg catgcccctc aaccgtttag accgggaaac
      661 ctctggagtt gcagtgcttg ctaaaagtgg aagtattgca tcagcatttt taaaatattc
      721 acatactatc gccaaacaat attatgcgat agttaccggc aattgttctt tttctgaaat
      781 aacagttgcc atgcccatag gattgtcccc cacttcatca atcaggaaaa aacgctctgc
      841 ctatacagac gcacactggc aggcacaaac acacatcaaa gtgcttcaac actatgacac
      901 atgcacgtta attgaagcca cacccaaaac agggcggact catcagatac gcgtccattg
      961 cgagcatatt ggccacccca ttattggtga taccctttac tgtaacagcg atactacatt
     1021 tattgaatat atcaaaaaca aaggtgctct attgcgtgaa tttcaacgct gcgctttgca
     1081 cgctcacata tacaagtttt atcatccatt cctacaaaag atgattgcaa taaaagcacc
     1141 aatgccacac gatatgcata catttttatt acgattagat aaaaactatg gcacaaccaa
     1201 caataccacc aaaagctaaa ctttttattg gactgttaac ctcccatgac gaggtgttat
     1261 ttcatgtcat caatatatta aaaaaaaaat ttggggttat tgatcttcaa tctgaaccca
     1321 ttccgttttc tcatactgat tactatagta ctatcgggaa aaatctttac aaggtactta
     1381 ttggttttaa caggcttata aaaagagaag aaattgctaa aattaaacta tttaccaaca
     1441 aagttgaaaa taaattctta aaaaacggca accgaaccat caacatagac cccggttacc
     1501 tcaccctgtc caatgttttt ttggtttcat gcaaagatta ttttcataga atatatattg
     1561 gcaggggaat atatgcagaa aatgaatatc ggtatgttgc taaacactat catccatggg
     1621 aatggacata tcccgattat cgaaaaaaag agtacctcca gttttttgaa acactcagaa
     1681 gcatctatta cgagcagata aaaaaataat agtaactgcc atcagtacaa aaaaaattgc
     1741 ggcaatggtt ccaccggttt taattggtaa agctgtgtaa gctgtgctgc aacctgttca
     1801 ccattgtcga tggaaccaaa tatagcaaaa aaaagctgat tggaaatatt tttattaatt
     1861 gtgtgtatac aaagcgcctg aaacaccttt gcaaacagat catccttttt aatttttaat
     1921 acatcagtaa atactttgag agctttttca taatttttct ggttaacatt aataatacct
     1981 gtaagcattg ctgcaaaata atttttttta tttatcgtta gtgcagtagt acagtcattc
     2041 agcgcctttt cataatttcc caaaaaataa tgcgctaatg cacgattata atacatagaa
     2101 tcatttttca tcttcagttg tatcacaata ttaaaatatt caattgctga tgcaaaatca
     2161 tttaattgca tagcactgag ggcacataga taatacccct gaacaaaatt tggtttgtgc
     2221 tctacaacct ttttgctcca gtttagcgcg tttgaaaaat caagtaactg ataatagaca
     2281 cctgctatat tatattctat aaatgcgtta taggtattgt tactgtttgc ctcacgaaag
     2341 aactgcaatg ctgtagccca ttgactgcgc aatgcatgga tatatcctgc agcattttgc
     2401 gcacgcgaaa attttggttc cattgccagt gcaataaaaa gttcttttaa tccatcatca
     2461 tatttttttt tattaatcaa tgttattgct ttaaaataaa aaacttcttt tgttggtgga
     2521 aagtccttca taaaattatt tatatgcttt tccattaaac catagtagcc atttaaatat
     2581 aatgtattga tagtagccag aagatcatac atggcaatat aatcgatgca atgttcaccc
     2641 tcattatggt gatataataa cgctgtatca cgtcgcgaag atgcaaactc ctgtgattga
     2701 ataagaagta acaaaaaaac aataataatt aattttttca tgtatacctt tttatgctaa
     2761 aaaggctgcg cgctgcacag ccttttttat caatgcattg catacatcat ataaaaatct
     2821 ggcactgaac agttatcctc ttgtttttat aatcatctgc ctcatccttc aattcctgag
     2881 catattctac cttaacgttg gcgctatgcc cttttaagta atagttcaat ccaccagcaa
     2941 tctcggacac cttgtttttg tcacctgcta atgattcggt atctttataa taaaaacgag
     3001 caactggctg ccatgtgtta ttgatcaaga aaccaccttc tgcaaaaaac atctttgctt
     3061 tgtcagcacc ttgagtaccg gcagcaacag ggtctatatc aaaaaaagga ctatttttta
     3121 catatacata cgctgcctgc agtgttagcg cattgtttgt accaatagga tgatcaaaat
     3181 aggcatcgac tgtccatgca taataatttt ttgcatcact gccatcatca taagcatcgg
     3241 gctgataatc caatccagcg ccaaagctta tgatgctttt tttacccaga tagttgccgc
     3301 tataaaagaa accatcctca gcatctttaa gattaatctg aatacgtccg gttactcgtg
     3361 gcatgtcatc ttcattaaca actgtgtttg tcgttgtatc taattttcta tcctgtccat
     3421 cccatgctcc aactcggtaa tcaaccaatc cattgaaaag cagccctctc aattcaacac
     3481 cagtatcacg ccacacattg ctaccgacag ggacaacggc tgcggtataa tcggcacaca
     3541 aaagcgttgc ggctgactga cggtcatgat gcatgaatgg taaaaggata tgaccaaacg
     3601 ctatcttcag ttcctttgca atagtaaaat caatatacgc atcctgagtg tataaatttt
     3661 ttgactcatt accctcctgg tttggcgcat cggtttccat aaaaaactca acattctgag
     3721 cagcctgtcc tttcacaatg atacgggagc gtcgcaactt tgtatctttt gaccagtaat
     3781 cctcatcgcc tgtggcagtg gcgccttctt tttgcaatga atatgcctgc acctgtaaca
     3841 gatagtggag ttctataaac tgatcatcac ccaacgttag cttagcaata ctatccgcag
     3901 caaacaccgg tgcagctacg ccagcaacca taacaatcgc aactaaaaat tttaaaaaac
     3961 gcatacactg atcctcctta aaaatttttt agattttttg caaaaacatt ggtaaagata
     4021 ataatttttg cttttgtatt tcacagggtt attttttcaa tgattttttg tattaattgc
     4081 cctgtgaata tctttttaat tcaattgaat aacctcaaca attgtagcgt taaccatcca
     4141 taggaaatca tgctcatgcg gttttcaaac tgtttttgag gcattcctta ttggtactgt
     4201 aacgccctct ttgcctgttc attgagccaa aatattgttg tattttagga gtaaacaata
     4261 tactactgtg gttttgttat ccatactgtt gctggggagg tatttctatg aggtttgcat
     4321 accatttcaa aaaatggcat cttttttatc tttttctgat attgatagcg gcatactaca
     4381 tagtggcaac gcatccattg ggaacatata atgcctatcc cgataaacta tcgcccaaag
     4441 aaaaggaaag ctcactctcg acattgccgc ttgataccag aagcccctgt ccaaaattca
     4501 gggctaacgg actgcaaaac ggcagaacca ctgtgcaatc aaaggcatcc atggtgcagc
     4561 catgggaatt taaaacaggc aaaggtattt tcagctcgcc tgttgttgat gaag
//
LOCUS       DAOSOX010000187         4566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_18998_length_4566_cov_8.639991, whole genome shotgun sequence.
ACCESSION   DAOSOX010000187 DAOSOX010000000
VERSION     DAOSOX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_4566_cov_8.639991"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLX22_10670"
     CDS             complement(<1..728)
                     /locus_tag="PLX22_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20400.1"
                     /translation="MQTKTKKTPLHNTFCSYCEYTGIVRNPNFEQIGEEPLVPCPRCV
                     LTKCQCNGLPPYFIEKDGMISDCDCRAIRMKINRIKTIYAKSGIDKKYQWRFFNSFEA
                     NSKLAANAKNIAYDLVSKFPDVRKGLFLWGNPGTGKTLLSSIILTELIIRHAIEGRFI
                     KISRTFFNKIRATFSEGSATYGTAQEIEYELQNADILVVDDFGVQRDSAWEQETLYNL
                     VDARYEAEKFTLFTSNVDPYRALA"
     gene            complement(949..>1191)
                     /locus_tag="PLX22_10675"
     CDS             complement(949..>1191)
                     /locus_tag="PLX22_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20401.1"
                     /translation="PLRTQDERLSYSTIADIVDMELAGIAYASYKFKKQCYSIKYISD
                     TGQHTTHQTIMNNIINFSEKTVDTILEILFSHSLIK"
     assembly_gap    1192..1291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1292..1612)
                     /locus_tag="PLX22_10680"
     CDS             complement(<1292..1612)
                     /locus_tag="PLX22_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20402.1"
                     /translation="MSKPAIAIQCATHTEAKPFINYFHCTLLQQKPFKIFLGDSSLYI
                     LIGGIGITKAAIAATYICLVYDVQYIINIGAAGALALQLLPGDICCVKSVIDNSRYNL
                     TTLEP"
     gene            complement(1594..2382)
                     /locus_tag="PLX22_10685"
     CDS             complement(1594..2382)
                     /locus_tag="PLX22_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOT20403.1"
                     /translation="MKTLAFSSCPNDTFIFHALVHSLININGLSFSTHIDDVESLNHA
                     ALNQEYDITKLSFHGFLHVQDNYELLDAGAALGFGCGPLVITRKGLNNIQHAVIATPG
                     IYTTAHLLFRLRFGNSNEFVHVRFDEILPGIQQGIFDAGVIIHEGRFVYQNYNCDMLI
                     DLGKWWEDVTGLPIPLGCIALHKKHSAQKSLIEKMIRNSIDYARRHPHDSKEYIRMLA
                     QELDETVIQQHIDLYVNDFSVSLGHTGNKALATLREMAQCQNLL"
     gene            complement(2387..3073)
                     /locus_tag="PLX22_10690"
     CDS             complement(2387..3073)
                     /locus_tag="PLX22_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20404.1"
                     /translation="MTIDEAIEQNKKIIIKAYSVSESIEYFLKVALTKVLHNYSCDML
                     LIPLYTSVKELVVNAVKANHKFIYFEGRKSYNSVFFDSEKALQLFKLEMTRGDARYFD
                     LLARKQDLYAMTTMSVIDRILYIDVENPTPMSDIEKEAVVKKLTIAQDCQDITDYFIQ
                     AQEDDYKEGAGLGLVLITMMFKGMGLSQKHFFIDSDFEKTIASMHVPLTATTIQEYKK
                     RLEETTPKSA"
     gene            complement(3124..3540)
                     /locus_tag="PLX22_10695"
     CDS             complement(3124..3540)
                     /locus_tag="PLX22_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT20405.1"
                     /translation="MNEKMLASPCGIFCAACPRFYKFGICKGCRTDSLHDNCEIYDCC
                     IRIGGMDFCFECDCFPCERLKQFSNYHHGENFAHYRHIIIDNLRQIKEMGVEKWYVIM
                     NQKFMNGEYGIQQRNPDGSFDISPCLCCKSTNHKKP"
     gene            complement(3809..4396)
                     /locus_tag="PLX22_10700"
     CDS             complement(3809..4396)
                     /locus_tag="PLX22_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20406.1"
                     /translation="MKKIYITIIFLFLVLNPLYSQLQYKHDQSFIPDFVVSQFAGYIG
                     FVSSGVGYNFYDESLKLELLYGYVPKSAGGISIHMFTCRGSYSLSHYRTFSGFLFSPL
                     NFSFFVNYARGNQYVLRWSPNYPKRYYRPTSIYTGESIGMSIKKSCENSIIKGYEFYG
                     DVVTMSEYLYEYCKNETVTLKDIISLAFGVRFYFL"
BASE COUNT         1385 a          792 c          817 g         1472 t
ORIGIN      
        1 tcagccagcg cccggtatgg atcaacattt gaagtgaata gtgtaaattt ttctgcttca
       61 tagcgggcat caaccagatt atacaatgtt tcctgctccc atgccgaatc acgctgaaca
      121 ccaaaatcat caaccacaag tatatcagca ttttgaagtt catattcgat ttcctgggct
      181 gtcccatagg tggcagagcc ctctgaaaat gttgcccgga ttttattgaa aaatgtcctg
      241 gatattttta taaatctacc ttcaattgca tggcgaatga taagctctgt aaggataatg
      301 gacgaaagaa gggtttttcc ggtgccggga ttaccccata aaaataatcc cttccgtaca
      361 tcaggaaatt ttgaaactaa atcatatgct atattttttg catttgctgc taatttagag
      421 tttgcttcaa atgagttgaa aaagcgccat tggtactttt tatcgatacc tgactttgca
      481 tagattgttt taatgcgatt gatcttcata cgtattgcac ggcaatcgca atcggatatc
      541 atgccgtctt tttcaataaa ataggggggt aatccattgc attggcattt tgtcaacaca
      601 caccgcgggc aggggaccag cggctcttca cctatctgtt caaagtttgg attgcgaacg
      661 atccctgtat attcacaata cgagcaaaaa gtattgtgca aaggcgtctt tttagttttt
      721 gtctgcatcg ctactccgat gaagtataat tgctacaatt gatatactat agaaacagtc
      781 tatatatttt caacgacaat ttcaaatatt ttgttatgag taaaatcttt attagtaaat
      841 acataaatga atcaattcaa aatacagtaa gtttagcaga tatgctggta aataatgcaa
      901 agaaagttga aaaaatggca aatgagttat tatcataatg attgtgattt atttgattaa
      961 tgaatgagaa aacagtatct ccagaattgt atcaacggtt ttttctgaaa aattgataat
     1021 gttattcatt attgtttggt gtgttgtgtg ttgtcctgta tcgcttatat attttataga
     1081 atagcattgt tttttaaatt tatatgatgc atatgcaatt ccggccagct ccatatcaac
     1141 aatatctgca atggtgctgt atgataaacg ctcatcctgt gttctcaaag gnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naggttctaa cgtggtgaga ttatatcgtg
     1321 aattatcgat aacagatttg acacaacata tatctcccgg caaaagttgc aaggcaagtg
     1381 ctcctgctgc gcctatgtta ataatatact gaacatcata tacaaggcag atgtatgttg
     1441 cggctattgc tgcttttgta atgccaattc cgccaatgag tatatacagg gagctatcgc
     1501 ccaaaaatat tttaaatggt ttttgttgca ataaagtgca atgaaaataa ttaataaatg
     1561 gttttgcttc tgtgtgtgtt gcacattgta tcgctatagc aggttttgac attgtgccat
     1621 ttccctgagt gtagccaaag ctttattacc cgtatgtcct aatgatacgc taaaatcatt
     1681 aacataaaga tcaatgtgct gctgtattac tgtttcatca agttcctgcg caagcatacg
     1741 gatatattct tttgagtcat gtggatgcct gcgtgcataa tcaattgaat tacgtatcat
     1801 tttttctata aggctttttt gtgcactatg ttttttatgt agtgcaatac atcccaatgg
     1861 aatgggcagc ccggttacat cttcccacca ttttcctaag tcaataagca tatcacaatt
     1921 atagttttga tatacaaagc gcccttcgtg gattataacc cctgcatcaa aaataccttg
     1981 ctgaatgccg ggtaaaattt catcaaatct gacatggaca aattcattac tgttaccaaa
     2041 gcgcagtctg aataaaagat gtgctgttgt gtatataccc ggtgtggcta ttacagcatg
     2101 ctgaatattg tttaatcctt tccgtgttat cacaagcggt ccgcagccaa atcctaaagc
     2161 ggcaccagca tccagaagtt cataattatc ctgtacatgt aaaaatccat gaaacgatag
     2221 ttttgtaata tcgtattcct ggttcagggc ggcatgattt aatgattcaa catcgtcaat
     2281 atgggttgaa aacgataatc cattgatgtt gatgagtgag tgtacaaggg catgaaaaat
     2341 aaaggtgtcg ttggggcatg atgaaaaggc caaagttttc atggtatcag gcactcttag
     2401 gtgttgtttc ttcaaggcgc tttttatatt cctgtattgt ggttgcagtt aacggtacat
     2461 gcatggatgc tattgttttt tcaaaatctg agtcaataaa aaaatgtttt tgtgataagc
     2521 ccatgccttt aaacatcatg gtgataagaa ccagtcccaa ccccgcacct tctttatagt
     2581 catcttcctg tgcttgaata aaataatcag tgatgtcctg gcaatcttgt gcaattgtta
     2641 acttttttac aactgcctcc ttttctatat cgctcatggg ggttggattt tcaacatcaa
     2701 tatagagtat tctgtcgata acagacatgg ttgtcatggc ataaagatct tgcttacgag
     2761 ctaatagatc aaaatagcga gcatcaccgc gggtcatctc taatttaaaa agttgcagtg
     2821 ctttctcact atcaaaaaaa acggaattgt atgattttcg tccttcaaaa tatataaatt
     2881 tatgattagc tttaacagca ttgactacca gttcttttac gctggtatag agcggtatca
     2941 acagcatatc acagctatag ttatgcaaaa cttttgttaa agcaactttt aaaaaatatt
     3001 ctattgattc gcttaccgaa taggccttaa tgataatttt tttattttgc tctatagctt
     3061 catcaatagt catatactcc ctcacatcat ctttttaaaa gatactatca taagtggttt
     3121 ttgtcaaggt tttttatgat ttgttgattt acaacataaa cagggggaaa tgtcaaaaga
     3181 accgtctgga tttctttgtt gtatgccgta ttcgccattc ataaatttct gattcatgat
     3241 aacataccac ttttcaacgc ccatttcttt aatctgtcgt agattatcaa tgatgatatg
     3301 gcggtaatgg gcaaaatttt caccatggtg ataattgctg aactgtttaa ggcgttcgca
     3361 ggggaaacaa tcacactcaa agcaaaaatc catgccacct atgcggatac agcagtcata
     3421 aatttcgcag ttatcatgaa ggctgtcggt tctacaaccc ttgcagattc caaatttata
     3481 aaacctggga caggcagcgc agaagatacc gcagggggat gctaacattt tttcattcat
     3541 agctattgtt gcaaatacac tttattaata aaagcgtatc atttttttta ttgtttccaa
     3601 aaaaattgct acattttttc agtggataaa ccataatgct gctgtgcaag tatcaagcga
     3661 tagtgaaaat gcggtacaat ggtgaagcaa agaattttgc tattttgatc aatgataaat
     3721 tcttcggctg ccaacgtttt gtcagtatgg caaaaatgct gtgttcctaa gaatgacaac
     3781 gaatacgtac ttttgttgtt gtaaaggttt ataaaaaata aaatctaaca ccaaaagcaa
     3841 gacttataat atcttttaat gttactgttt catttttaca gtattcataa aggtattcac
     3901 tcatcgtcac aacatcgcca tagaattcat agcctttgat gatggaattt tcacagctct
     3961 tttttatact catgccaata gattctcccg tgtaaataga agttggcctg taataccttt
     4021 ttgggtaatt tggtgaccat cgtaaaacgt attggttacc gcgggcataa ttaacaaaaa
     4081 atgaaaagtt aagaggggaa aacaaaaatc cactaaaagt tctatagtgt gataatgaat
     4141 aagaacctct gcatgtaaac atatggatac taattccgcc tgcagatttg gggacatagc
     4201 catataataa ttccagctta agactttcat cgtagaaatt gtatcccact ccggaagaaa
     4261 caaaccctat atatccagca aattgtgaaa ctacaaaatc agggataaat gattgatcgt
     4321 gtttatactg tagttgtgaa tacaaaggat ttaatacaag aaataaaaaa ataatggtta
     4381 tatatatctt cttcatgatg tttcgttaaa atccaacaat ttcatggtct ataccggttg
     4441 cccttacatg gacgagataa aatttatcat ggactattgc atttgcaatg agcatagcag
     4501 ttttgctggt aaaatccggt atatagtagt gtctatgctt gtgtccatgg agggaaagcg
     4561 caacat
//
LOCUS       DAOSOX010000188         4500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19357_length_4500_cov_8.707312, whole genome shotgun sequence.
ACCESSION   DAOSOX010000188 DAOSOX010000000
VERSION     DAOSOX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19357_length_4500_cov_8.707312"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLX22_10705"
     CDS             <1..719
                     /locus_tag="PLX22_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HOT20407.1"
                     /translation="HVRIQAAFQEYVDNAVSKTVNFPHSATVGDIETVYMLAYHLGCK
                     GITVYRDGSRETQVLQVEKGKQPVPIMFNIQPRPRKEVTWGKTLKMNTGCGSLYVTIN
                     EDEYGLFEVFATMGKAGGCAASQAEAVSRLISLGLRSGIEPEQIIKQLKGVRCPNQAW
                     VMGGRIYSCADAIAKAIERYIHPDAVQEQNEDIHKNIAETNGKGSDTVMVGVCPECHG
                     PLEFESGCSVCRICGFSRCG"
     gene            723..1541
                     /locus_tag="PLX22_10710"
     CDS             723..1541
                     /locus_tag="PLX22_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOT20408.1"
                     /translation="MYQVYFTADIDEQLSEKLALYAQSISILYDEKAIIALFDEEDEI
                     PSFAKDYILQINNLEEEVWKYRYLDYLTGVAITPSIYVYPTTKALGGDSSYTYTIALN
                     PKDAFGDGTHPTTRMCIQQLEKIVNTFSLEERRSLTLCDIGTGTGIIAIVAAMLGIGT
                     VEAFDIDTTSVERAKENAAQNGCNRILFFVSDIANVNTYKKYKIIVANLLSDIIEKNV
                     KNIVSLVEKGGTIILSGVGHRWVKGIIQLFQQCGCTPIHDESMSTWHCMVLKAK"
     gene            1544..2551
                     /locus_tag="PLX22_10715"
     CDS             1544..2551
                     /locus_tag="PLX22_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT20409.1"
                     /translation="MKIKNIKEVYPGIFRIVEYRSLKVTPPINLYIIAGSDGLLFDGG
                     YGDDNSIQIVGDAIDYVHSLNEKWRITRVLLSHTHPDHFAGLRRLQQQYHLKVMLTEP
                     MTHVIANRKSYIHANKEDGVLKKRYSTIKQRLAALPLEFFYQKMFKKLYGIQYVPNPD
                     IIISPQDTITVDNAKWKIIPGPGHCADHIMLYNKKQGILFGGDNVLRSIYTWLGPPRS
                     DLQAYMQTLHTCLQLPHLQCILGAHGKPVTNPRQRIEQIIEYRKSRTKQIYSIIANAP
                     NGGITFDRLMNMMYPKKNGHRWLGRGWIIVTIEYLVNKNALRFQDKKIIANPVDINSV
                     L"
     gene            complement(2535..3500)
                     /locus_tag="PLX22_10720"
     CDS             complement(2535..3500)
                     /locus_tag="PLX22_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT20410.1"
                     /translation="MDSNYRPWILLFAFLMLLPGCTSLGMRSIPVDELKKKYASASSR
                     FIVIEDVTIHYRDEGKKDAPVLLLLHGVCASLHTWDGWVALLKDDYRIIRVDAPGFGL
                     TGPISSGNYTPEYYVELINKMLEQLSIPELSIAGNSLGGYMAWQFALKYPQKVEKLIL
                     IDSVGFPQEMPWLLKFSTNALVRPFARSAMPRYFINKAVHEVYGDQSKVTKEVVDRYF
                     ELSQREGNKSAWIDIFMVMKEYSKREDLSKGLTDIHCPIMVMWGTKDIWIPYTTEFPK
                     WQKALPHATFKVYEGAGHIPMEEIPEVTAHDAFAFLTGKELTARN"
BASE COUNT         1401 a          825 c          989 g         1285 t
ORIGIN      
        1 ggcatgttcg tattcaggca gcgtttcagg aatatgttga taatgcagtt tccaagacgg
       61 taaacttccc tcattcggca acggtaggag atattgaaac ggtgtatatg ctggcatatc
      121 atctgggatg caagggcata actgtttatc gggatggttc acgtgaaacg caggtgctgc
      181 aggttgaaaa gggaaagcag cctgtgccta ttatgttcaa tattcaacca cgcccaagga
      241 aagaggtaac atggggtaaa acactgaaga tgaatacggg gtgcggctcg ctctatgtta
      301 ccattaatga agatgagtat gggttgtttg aggtgtttgc aaccatgggc aaggcgggcg
      361 gatgcgcagc cagccaggcc gaggcagtaa gcaggcttat ctcgttagga ttgcgatcag
      421 ggattgaacc tgagcaaatc ataaagcagt tgaaaggagt acgctgcccc aatcaggcat
      481 gggttatggg tggaaggatc tactcatgtg ccgatgccat tgcaaaggct atagagcgat
      541 acatacatcc tgatgcagta caggagcaga acgaggacat acacaaaaat attgccgaga
      601 ccaatggtaa agggagcgat acggtgatgg ttggcgtgtg ccctgaatgt catggcccgc
      661 tggaatttga gtcaggatgc tcggtatgcc gtatatgtgg tttttcacga tgtggttagc
      721 ctatgtatca ggtttatttt actgctgata ttgatgaaca gttaagtgaa aagttagcgc
      781 tctacgcgca gagcatttca atactgtatg atgaaaaggc aattattgcg ttgtttgatg
      841 aagaggatga aataccatca tttgcaaaag attatatttt acagataaac aatttagaag
      901 aagaggtctg gaaataccgg tatcttgatt atcttactgg tgttgcaata acgccatcaa
      961 tctatgttta tcctaccaca aaagcattgg ggggcgatag ttcatataca tatactattg
     1021 ccttaaatcc aaaggatgcc tttggtgatg gcacccatcc cacaacgcgc atgtgcattc
     1081 agcaactcga aaaaattgta aatacttttt ctctggaaga acgacgatcg cttacactgt
     1141 gtgatattgg caccggtacc ggcatcatcg ctattgtagc ggcaatgctt ggcataggaa
     1201 cagttgaagc atttgatatc gatacaacct cggtagagcg cgcaaaggag aatgctgcac
     1261 aaaatggctg taacagaata ttgttttttg tttcagatat tgcaaatgtt aatacatata
     1321 aaaaatataa aattattgtt gcaaatttat tatcagatat tattgagaaa aatgttaaaa
     1381 acattgtatc attggttgaa aaaggtggta caataatact aagcggtgtt ggccatcgct
     1441 gggttaaagg gataatacaa ttatttcaac agtgtggatg tacaccaatt catgatgaaa
     1501 gtatgagcac ctggcattgc atggtattga aagcgaaata gatatgaaga taaaaaacat
     1561 taaggaagta tatcccggta tattcagaat tgtagagtac aggtcactga aggtaacgcc
     1621 accaattaac ctttatatta tagctggcag tgatggattg ctgtttgatg gcggttatgg
     1681 tgatgataat tcaatacaga ttgttggcga tgcaattgat tacgtacata gccttaatga
     1741 aaagtggcgc atcacgcggg tactgttaag ccatacccat ccagatcatt ttgcaggatt
     1801 acgacgattg cagcagcagt accatttaaa ggtaatgctt acggaaccta tgacgcatgt
     1861 tatagcaaac cggaaaagtt atattcatgc caataaagag gatggagtat taaaaaagcg
     1921 gtattcaacc attaaacagc ggttagcggc gctgcctctg gagttttttt accaaaaaat
     1981 gtttaaaaaa ttgtacggca tacagtatgt tcccaaccct gacatcatca tatcaccgca
     2041 ggatactata acagtagaca atgcaaaatg gaaaatcata ccagggccag gacattgcgc
     2101 cgatcatatc atgctttaca acaaaaagca gggcatactg tttggcggcg ataatgtttt
     2161 gcgcagtatc tatacatggc ttggacctcc ccgctcggat cttcaggcat acatgcaaac
     2221 cttacatact tgcttgcagc ttcctcattt gcaatgtata cttggtgcgc atggtaaacc
     2281 ggttaccaat ccgcggcaac gcattgagca aataatagaa taccgaaaat cacgcacaaa
     2341 gcagatttac tctatcattg caaatgctcc aaacggtggg attacctttg atagattgat
     2401 gaatatgatg tatccgaaga agaacggaca ccgctggctt gggcgcggct ggataattgt
     2461 aaccattgaa tatcttgtaa ataaaaatgc cctgaggttc caggataaaa agattatagc
     2521 caacccggta gatattaatt ccgtgctgtg agttctttac cggttaaaaa tgcaaaggca
     2581 tcatgggcgg ttacttcagg tatttcttcc atcgggatgt gtccagcacc ctcgtacacc
     2641 ttgaaggttg catgcggcag tgctttctgc catttaggga actcagtagt atagggaatc
     2701 catatatctt ttgtgcccca catgaccatg atgggacagt gtatatcagt taagcctttt
     2761 gaaaggtctt cccgttttga atactctttc ataaccataa agatgtctat ccatgcgctt
     2821 ttatttcctt cccgttgcga aagctcaaaa tatcggtcaa ccacttcctt tgttaccttg
     2881 ctctgatcac catacacttc atgaacagct ttattgataa aatagcgagg catcgcactg
     2941 cgcgcaaatg ggcgtaccaa agcatttgtt gaaaatttta aaagccacgg catttcctgc
     3001 gggaagccaa ctgaatcaat taagattagt ttttcaactt tttggggata ttttaacgca
     3061 aattgccatg ccatataacc gccaagcgaa ttccctgcaa ttgaaagctc tggtattgaa
     3121 agctgctcaa gcattttgtt aataagttca acataatact cgggtgtata attgccggat
     3181 gaaatgggcc cggttaaacc aaaacccggg gcatccacgc ggattattct gtaatcatct
     3241 tttaatagag ctacccagcc gtcccaggtg tgaagagaag cgcaaacccc atgtaaaagc
     3301 aacaaaacgg gtgcatcctt tttcccttca tcacgataat ggattgtaac atcctcaatc
     3361 acgataaagc gtgaagaggc gctggcatat tttttcttta attcatctac gggtatgctg
     3421 cgcatcccta atgaggtgca gcctggcaat aacattaaaa aggcaaagag caatatccat
     3481 gggcgatagt tactgtccat gataacctcc gataaaaata taataatgat aacaatataa
     3541 tttcagaatg ttgtgcacat gtataaatac aaccgacgaa tcagtattta tgtgactgct
     3601 ggaaaaagtc aataataata aaagggttac tcaataaatt tttatcagtg aataacatgt
     3661 cattcagaat gacgcagtac tgaagctact attcgttcat tctcacaaaa gacaatatcc
     3721 ttcgcctgcg ttcagaataa tatgccgctg cggtcattcc gggcttgacc tggaatctca
     3781 ataattaacc ttagatcttg aaacaaggta aggacataat tcaggacgac tttctgcgtt
     3841 taaggatgac acagtggaag ataaaaatta atttaaaaaa ttttttataa attttgttaa
     3901 atgacttgac ttttccataa tcattgtatt tattaataac tagaagagta agctttaggt
     3961 tttttacaac tctaagttct tactgttctg gccgcaagta cacttgcgga aagatgagtt
     4021 tggtacacaa tgtaccacag gtttaagtgt tatggtttta cagaaaagaa agaggatgaa
     4081 gttcctgaaa tttcatcctc tttctttttt attgcagctc ggaaacgtat tgacagacac
     4141 taagcgatgc tatagttgat tagtgggagc aattagagaa aagcaacatg actgtataat
     4201 gcttattgat aaatgatgaa aatatccatg tagtatgata tggattcaac gaaatcatta
     4261 ttaccattat cagagtgggg agatattttt gtaaaaacct ttggttttta catagtctgt
     4321 tgtttcaggt tctaatgttg tatatatgga ttgcgggtca cgcccggaat gacgttgagg
     4381 agtaatcata ttttcagagg agcaggtaaa ggtaatttta tgaaaaggtc aggtatacta
     4441 taactctata tcgtcgataa ggcaatggta tgtaatcatg gatgagtgta tgctttctat
//
LOCUS       DAOSOX010000189         4468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19546_length_4468_cov_8.787899, whole genome shotgun sequence.
ACCESSION   DAOSOX010000189 DAOSOX010000000
VERSION     DAOSOX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19546_length_4468_cov_8.787899"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLX22_10725"
     CDS             <1..971
                     /locus_tag="PLX22_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT20411.1"
                     /translation="CQLSQHLAQAIRYHHVDYMQRKENSIGVTIPLTSYIIHVADRVD
                     VLINRRKDYLLQKDDVLATIKKYSGKRFDPEIVQCFEEVSAKESFWFDLQFDNIDDKI
                     QSLVFHNPVLSLDEIYEISRLFTKIIDFRSRFTATHSTSVANIARELGLVLNISKREC
                     LMLDIAGHLHDIGKLAIPLTILEKQGPLTKEEWMIMKRHAYFTYTILTVIDSPEFRII
                     NEWASYHHERQDGRGYPFKITKDNLTIGSRIMAVADIFTALAEERPYRSAMDDNKIVS
                     AMNKAKKDLLDPMIVEAMLDNYSHFKELRSEAFRNSLKEFKEQFDF"
     gene            1178..2995
                     /locus_tag="PLX22_10730"
     CDS             1178..2995
                     /locus_tag="PLX22_10730"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT20412.1"
                     /translation="MKKINEILNKLSIKARVTVGVIVVLIVSLLFTNIFWRISFIGEV
                     ERQFIDKARSICTMGEAMREYMADNWERQLYDTDYLKKDIKGKFIYSVPVFSSIITMQ
                     KKAEVLGYTFRVPKEYPRNKANTPTPLEQKVLDELKTKNVPEYFLLDYKNNVLRYFRP
                     VRLTKDCLICHGDPAQSKALWGRDDGKDPTGGTMEGWKAGEVHGAFEIVYSLKEYVAA
                     RFQILIISVVANLIILGLAIVLIRRVVRKGLSPLDEMVKSLEEMNRGEGDLTHKIAIE
                     SDDEVGKLALLFNQFIDNLRLLILAVKDAADHVASSSTEMTQSSQQLANVAQDQAASI
                     EETSSAMEEIKATIDSVSENAKHQAKKADATRNSMEYLADAINRINHNAQEAYTMADE
                     THDYAKDGESILGSTVSGMKEINESSKRITEIVTIISDISDQINLLSLNASIEAARAG
                     EHGKGFAVVAEEISKLADQTAESSKEINKLIHETNNKVNAGSGLVERTAESLRKIIEN
                     VKTTAVLMENIAQSSQELNRMSNDVKGDVEEVNKMSEEISMMMEEQSASTNEIIKAIN
                     QINEITQVVSSGSEELAAASEELSSQAESLNQVVKKFNT"
     gene            complement(3008..3361)
                     /locus_tag="PLX22_10735"
     CDS             complement(3008..3361)
                     /locus_tag="PLX22_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20413.1"
                     /translation="MKKIYCILLITGMMFCAHACAHVNQPDTKVTPVKQQQKFAQNVV
                     PCEYLFTVDETCDVQCLYSLLVKDYSITNIKAIGNNLFLVTFTIDPGMDALLSMYNNI
                     STIKAIQPNFKYKLQ"
     gene            complement(3376..>4468)
                     /locus_tag="PLX22_10740"
     CDS             complement(3376..>4468)
                     /locus_tag="PLX22_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOT20414.1"
                     /translation="VRVLNAVGEGTTANIVSGINFAVSRGAKVINMSLGGNLSDAVFS
                     NAITNAQNNGVLVVVAAGNKGYNLNTAGKDSYPCEYTQDNIVCVAALDQAYALADFSN
                     YGATGVDVGAPGTNIVSGYFGNYQAITTDDFSSGWGLTGWAQQANLGGFPALTDPTNF
                     NYSNEYLAYANHKAYKSFSLSGYTNAILLFEAYIDIEGGYDFFSVNYKIGTGDPFVTG
                     QQLLRISGSFSNPWYHDYYPFPFDISSYTGTNCSIGFRLQSDGSYQYTGVAIRNFAIV
                     NANTSVTNAYEVMDGTSMASPYVAGLAAMLIAFNPDYTYQDVANALKNGGETVASLAG
                     ITTTGKSINAWGSLKYINTPAGLSAQQIQ"
BASE COUNT         1481 a          793 c          931 g         1263 t
ORIGIN      
        1 catgccaatt gtcacaacat ttagcgcagg ctatacgcta tcatcatgtt gattacatgc
       61 agaggaagga gaatagtatt ggggtaacta tccccctgac ttcctatatt attcatgtag
      121 cggatagagt agatgtgctc attaacaggc gtaaggatta tcttttacaa aaagatgatg
      181 tactggcaac aataaaaaaa tattctggca aaagatttga tcctgaaata gtgcaatgct
      241 ttgaggaagt atctgcaaaa gagtcattct ggtttgacct tcaatttgac aatatagacg
      301 acaaaataca atcattggta tttcataatc cagtgctttc attggatgaa atatacgaaa
      361 tatccaggct gtttacaaag attatagatt tcagaagccg gtttacagca acccattcaa
      421 catctgtagc taatattgcc agagaattag gtttagtact taatatttca aaacgagaat
      481 gcttgatgct tgatattgca gggcatctgc atgatatagg gaagttagca ataccgttaa
      541 ccattctgga aaagcaaggg ccattaacaa aagaagaatg gatgataatg aagcgacatg
      601 cctattttac ctatactata ttgacagtaa tagattctcc tgaatttaga ataataaatg
      661 aatgggcatc atatcatcat gaaaggcagg atgggagagg ctaccccttt aaaataacaa
      721 aagataattt aactattggt tcccgtatta tggcggttgc tgacatattt actgctttag
      781 ctgaagaaag gccatatcga agcgcaatgg atgacaataa gatagtgtct gcaatgaata
      841 aagctaaaaa agatttactg gacccaatga ttgttgaagc aatgctggat aattattctc
      901 attttaaaga attaagatct gaagcatttc gtaattcatt aaaagaattt aaagaacagt
      961 ttgattttta atataattta caaacaatat tttcattgaa taatgtattg gattgttata
     1021 tgattgatta ataaaggcta aaaagcagat tccggatcag gtccggaatg aaagtggtta
     1081 atgatgccat tttatgaaga caatgccccc ttgcttacga aggagaatgg tcagggcgag
     1141 gttaataatt atagtttctg aggaggagat gctgtctatg aaaaaaataa atgagattct
     1201 caataaacta tcaatcaaag ctcgtgttac ggttggggtg attgttgtgc ttattgtaag
     1261 cttgttattt accaacatat tctggcgcat aagttttata ggcgaagttg aacgtcagtt
     1321 tattgacaag gcacgatcaa tatgcacaat gggtgaggcg atgcgtgagt atatggcaga
     1381 taactgggaa cggcagcttt acgatacaga ctatttaaaa aaggatatca aaggaaaatt
     1441 tatatactca gtaccggttt tttcatcaat tataaccatg caaaaaaaag cagaggtgct
     1501 tggctatact tttagagtac caaaggaata cccacgaaat aaggcaaata caccaacacc
     1561 attagagcaa aaggtgctcg atgagctaaa aaccaaaaat gtacctgaat actttttgct
     1621 ggattataaa aacaatgtac tgcgttattt caggccggta aggcttacaa aggattgttt
     1681 gatatgccat ggcgaccctg cacaatcaaa agcattgtgg gggcgcgacg atggaaaaga
     1741 cccaacgggc ggcaccatgg aagggtggaa agcaggagag gttcatggag cgtttgagat
     1801 tgtctattcg ttaaaggaat atgttgccgc acgattccag attcttatta taagtgttgt
     1861 cgccaattta atcattttag gtttggcgat agttcttata cgaagggttg taaggaaagg
     1921 attgagtcca ctggatgaaa tggtgaaatc gctggaagag atgaacaggg gtgaaggcga
     1981 tttaacacat aaaatagcta ttgagagcga tgatgaggtt ggaaaacttg cattgctgtt
     2041 taatcaattt attgataatc tgcgcttgct tatcctggct gtgaaagatg cagcagatca
     2101 tgtggcttcg tcatcaacag agatgactca gtcaagccag cagcttgcca atgtcgctca
     2161 ggatcaggca gcttccattg aagaaacatc ctctgccatg gaagagatta aggcaactat
     2221 cgactcggtt tcagaaaatg ccaaacatca ggcaaagaaa gcagatgcaa cccgaaactc
     2281 tatggaatat ttagctgatg ctatcaatcg tattaatcac aatgcccagg aagcgtatac
     2341 aatggcagac gaaacccatg actatgcaaa ggatggcgaa tcaattttag ggagtactgt
     2401 aagcggcatg aaagagataa acgaaagctc aaaacgtatt actgaaattg taacaataat
     2461 ttcagatatt tctgaccaga tcaatttgct ttcactcaac gcttcaatag aagctgcacg
     2521 agcgggtgag catgggaaag gctttgccgt tgtagcagaa gagatatcaa aattggctga
     2581 tcaaactgcc gaaagttcaa aagagatcaa taagcttatc catgaaacaa ataataaagt
     2641 aaacgcaggt tcagggcttg tggaaaggac ggccgaatca ctgcgaaaga ttattgaaaa
     2701 tgtaaaaaca acagcagtgc ttatggaaaa tattgcgcag tcatcacagg agctcaaccg
     2761 aatgagcaat gatgtcaagg gcgatgtaga ggaagttaac aagatgtctg aagaaatatc
     2821 aatgatgatg gaagagcaga gtgcttcaac caatgagatt atcaaagcta tcaaccagat
     2881 caatgaaatc acccaggtgg tgtcaagcgg ttctgaggaa ctggctgcag catctgaaga
     2941 gctttcctca caggcagaaa gtttgaatca ggttgtaaaa aaatttaata catagggaag
     3001 atgaatatta ctgtagttta tacttaaaat ttggctgtat agcctttatt gtagatatat
     3061 tattatacat ggacaataaa gcgtccatgc caggatcaat agtaaaagta accaggaata
     3121 gattgttacc tatggcttta atgttggtaa tgctgtagtc ttttactagt agcgagtata
     3181 agcactgcac atcacaggtt tcatcaacag taaataaata ttcacaggga acaacattct
     3241 gagcaaactt ttgttgttgt ttcaccggag ttacctttgt atcaggttga tttacatgtg
     3301 cacaggcatg agcacaaaac atcatgcctg tgattaacag tatacaatat atttttttca
     3361 tgcactacac acctattact gaatttgttg tgcagacaac cctgctggag tattgatata
     3421 tttcaatgaa ccccatgcat tgatagactt gcctgttgtt gttataccag ctagagaggc
     3481 aactgtttcc cctccatttt ttagtgcatt ggctacatcc tgataggtat agtccggatt
     3541 aaaggcaata agcatagcgg caagccctgc aacatacggg gaagccatag atgtgccatc
     3601 cataacctca tatgcgttgg tgacggaggt atttgcattt actattgcaa aattacggat
     3661 tgcaacacca gtgtattgat aacttccatc agattgtaat cggaatccta tggagcagtt
     3721 tgtaccggta tacgatgaaa tatcaaatgg gaatggataa taatcatgat accatggatt
     3781 actaaaactc cctgatattc tcaataattg ttgcccggtt acaaaaggat ctcccgtacc
     3841 aattttgtaa ttgacactga aaaaatcata acctccttca atatcaatat acgcttcaaa
     3901 taacaggatc gcattagtat agcctgataa actaaaactt ttatatgcct tgtgatttgc
     3961 atatgctaag tattcattgg aataattgaa attagttgga tcagtaagtg ctggaaagcc
     4021 gccaagatta gcctgctgtg cccaccctgt taatccccat ccactggaaa aatcatctgt
     4081 ggtgatagct tgatagttac caaaatatcc acttactata ttggttccgg gagcgcctac
     4141 atcaacccct gttgcaccat agtttgaaaa atcggccaga gcatatgcct gatcaagcgc
     4201 tgccacgcat acaatattgt cctgtgtata ttcacagggg tatgaatctt tgccggcagt
     4261 attcaggtta tatcctttat tacccgccgc aactactacc aatactccat tgttttgtgc
     4321 attggttata gcattggaaa aaactgcatc gcttagattt ccacccaaac tcatattgat
     4381 tacttttgcc cctctactaa cagcaaagtt tataccgctt acaatattgg ctgttgttcc
     4441 ttcacctact gcattaagta ccctcacc
//
LOCUS       DAOSOX010000190         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19603_length_4457_cov_7.725579, whole genome shotgun sequence.
ACCESSION   DAOSOX010000190 DAOSOX010000000
VERSION     DAOSOX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19603_length_4457_cov_7.725579"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..1535
                     /locus_tag="PLX22_10745"
     CDS             579..1535
                     /locus_tag="PLX22_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT20415.1"
                     /translation="MKVKFWGVRGSIPTPLNSDVIRDKIKQALQLASPKDISNEESID
                     TFLDTLPISIVGTYGGNTTTLEVRTSTDDLIIVDCGTGIKQLGHELLKTEFGNGKGFA
                     TILLTHTHWDHIQGFPFFAPFYVKGNRFNIYSPYNDIKERIEYQQVYTHFPINLEYML
                     AQKEFFTIDKESEIYINDVKIKNQSMRHPGGSFGFRFEENGKSFVFTSDCEFNIDEID
                     KIDTYKNFFENADVCVFDTQYTFEESINKIDWGHSSASIAIDIASMFNIKRLILFHHE
                     PDYNDQKLDTVLSNAKLYMGINPDRRGNLTINIAREGMEIEI"
     gene            complement(1536..2222)
                     /locus_tag="PLX22_10750"
     CDS             complement(1536..2222)
                     /locus_tag="PLX22_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT20416.1"
                     /translation="MKKYFVIGLGNFGFNIARSLEENGCEVLGMDVNKDLVQRAKDSI
                     SHAIIGDASDREVLESLAISDFDGSIISIGQDMAASILIALYLKEIGIKKIIVRAISE
                     DHGKILSLIGVDTVVFPERDMAIRLANKLALKNAMDYLPITDEYGILEVIPPKTFINK
                     SLKDLKLSTRYGCQVIGLKYFPNGNYNDISEKNVQIKIAPTADDVILENSVMIVIGKV
                     EDIERLQNAD"
     gene            complement(2229..>2663)
                     /locus_tag="PLX22_10755"
     CDS             complement(2229..>2663)
                     /locus_tag="PLX22_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT20417.1"
                     /translation="SPGSIAGGVKTSTVAVLYIYLISRLKGLKQIVIGERALSYDNIE
                     RATTLVIVSGIFIFCTTFILVLIPTLPIPEFLASLFEVTSAFGTVGLSMDFTPKTLPI
                     ERIILCIVMYAGRLGPLTLISALTSHRKTINIRYAEDHVMIG"
     assembly_gap    2664..2763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2764..3449)
                     /locus_tag="PLX22_10760"
     CDS             complement(<2764..3449)
                     /locus_tag="PLX22_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT20418.1"
                     /translation="MKKLQEYLSPIEKVLLSFVIVIFSGAFLLCLPVCTTHGITFIDA
                     LFTSTSAVCVTGLVVLDTAKDFTFIGQVVILLLIQLGGLGIMTFSVALLSLIGSSISI
                     KWRFTLQNMYSDVSKLPVRHVLTRIVIYTFSIEACIAFILFTQFIQQFPFRVAIWHSI
                     FHSVSAFCNAGFSTFSSNLMDYNGNTVVIISIGLDIILGGLGFLTLSELFSNKTFKLK
                     KLWKALTIHS"
     gene            complement(3493..3565)
                     /locus_tag="PLX22_10765"
     tRNA            complement(3493..3565)
                     /locus_tag="PLX22_10765"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3530..3532),aa:Val,seq:tac)
     gene            complement(3566..3832)
                     /locus_tag="PLX22_10770"
     CDS             complement(3566..3832)
                     /locus_tag="PLX22_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOT20419.1"
                     /translation="MTKHEIITRLHIKHEIPRDVVQQIVDGFVEEIVQSVQNGEKVTI
                     KGFGVFTRIRKAERKVFSPIAQREVVVPSKYVIDFKSSKNTAIE"
     gene            complement(3848..4123)
                     /gene="rpsT"
                     /locus_tag="PLX22_10775"
     CDS             complement(3848..4123)
                     /gene="rpsT"
                     /locus_tag="PLX22_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT20420.1"
                     /translation="MANIKSAEKRNRQNEKRRQKNSKIKSSVRTAEKKVRKSLENNSN
                     ETAQVLFKEFVKKIDAAAGKDILHWKTAARKKSRLAKKVNSVNAVSQ"
BASE COUNT         1488 a          811 c          680 g         1378 t
ORIGIN      
        1 ctcgggcagt tgtaggcgca atttccgtgc catgggtatt cctccgtgtt gttataataa
       61 ttgaaatccc tactatacat aacgccagag atgaaaaaaa ttaaaaagaa atggtgtatt
      121 ttttatactt tttgtgattt tttttaaaaa aacactaact ataaggggtc agagccttaa
      181 taacccccct tatcccccct ttgaacgaaa ggggggaatt gtatggagga attcaatctt
      241 tgattaatga taaatatgct aaaatctaca tctcttcctt tttgtgagac gaaacattat
      301 aaattattta tataacccat aatatcatac aaataatcag caacaaacat ctaacaatgc
      361 atatcttgca ttgtacagga aataatgggg tcagagcata tgttctgtat ctgcgatgtt
      421 ctgtgtctgc gtaagtacat acatatcaca ttgaaaattg gattacacat gaaaaaataa
      481 gaaagaaatt ggggccagag ccttataaaa aattattagt tgatttcttt atatggatat
      541 attttatata accatattat attgaggaga aaaatttaat gaaagtaaaa ttctggggcg
      601 ttcgaggttc aatcccaact ccattaaatt cagatgtaat aagagacaaa ataaaacagg
      661 cattacaatt agcttcacca aaagatatct ccaatgaaga atctatcgat acattccttg
      721 atacattgcc catatcaata gttggcacct atggaggtaa tacaacaaca ctggaagttc
      781 gcacctctac tgatgattta attattgttg attgcggtac aggaataaaa caattaggac
      841 atgaattatt aaagactgaa tttggtaatg gtaaaggttt tgcaacaata ttacttactc
      901 atacccactg ggatcatata cagggttttc cattttttgc ccctttttat gttaaaggca
      961 accgattcaa tatttattca ccatataatg atattaaaga acgaattgaa tatcagcagg
     1021 tctataccca tttcccaatt aatttagaat atatgcttgc tcagaaagaa ttttttacaa
     1081 ttgataagga aagtgaaata tatattaacg atgtcaaaat caaaaatcaa agcatgcggc
     1141 atccaggtgg ttcatttggg ttcaggtttg aagaaaatgg caaatcattt gtttttacaa
     1201 gtgattgcga gttcaatatt gatgaaattg ataagataga tacatataaa aatttctttg
     1261 aaaatgcgga tgtatgtgta tttgacacgc agtatacatt tgaagaatca ataaataaaa
     1321 tagattgggg acattcatct gcttctattg caatagatat tgccagcatg tttaacatca
     1381 agcgattaat tctattccac catgaacctg attataatga tcaaaagctt gatactgttc
     1441 tatcaaatgc aaaactctac atgggaatta atcctgatcg aagaggcaat ttaacaataa
     1501 atatcgcacg cgaaggaatg gaaattgaaa tataatcaat cagcattttg taaacgttca
     1561 atatcctcta ctttaccaat aactatcata accgaatttt ccagtataac atcatctgct
     1621 gttggcgcaa ttttaatctg aacatttttt tcgcttatat cattataatt tccatttgga
     1681 aaatatttta agcctattac ctggcatcca tatcgggtac ttaatttcaa atccttcaat
     1741 gatttgttta taaaggtttt tggaggtata acttccagaa ttccatattc atcagtaata
     1801 ggaagataat ccattgcatt ttttagtgct aatttgttag caagccttat tgccatatct
     1861 cgctcaggaa aaaccaccgt atcaacacca ataagtgata atatttttcc atggtcttcc
     1921 gagatagctc ttacaataat ttttttgatg ccaatctctt taagatataa ggcaatcaat
     1981 atgcttgccg ccatatcctg tcctatactt ataatagaac catcaaaatc acttattgcc
     2041 aatgattcta aaacttccct gtctgaagca tcgccaataa ttgcatgact tatagaatct
     2101 ttggctcgct ggaccagatc tttattaaca tccatcccca atacttcgca tccattttct
     2161 tctaatgaac gagcaatatt aaacccaaaa ttcccaagtc ctattacaaa atattttttc
     2221 atatttaact accctatcat tacatgatct tctgcatatc ggatattaat cgtttttcta
     2281 tgtgatgtca atgccgaaat caatgtcaat ggtcccaacc gtcctgcata cataacaata
     2341 cacaaaataa tccgttcaat aggtaaagtt ttgggagtga aatccatcga cagcccaact
     2401 gtaccaaatg ccgaagtaac ctcaaataaa gatgcaagaa attcaggtat tggcaatgta
     2461 ggtattaaga caagaataaa cgtagtacaa aaaataaaaa ttcccgatac aatgaccagc
     2521 gttgtagccc tttcaatatt atcatatgac aaagctcttt cccctataac tatctgttta
     2581 agccctttta aacgggatat aagatatatg tataatacag ccacagtact tgttttcaca
     2641 ccgccagcta tagaaccggg cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnncttgaat gaatagtcag agctttccag agtttcttta gcttgaatgt tttgttggaa
     2821 aataactcgc tcaacgttaa aaatccaagc ccacctaaaa taatatccag acctatactg
     2881 ataatgacaa cggtattacc attgtaatcc ataagattac tggaaaacgt ggaaaaacca
     2941 gcattgcaaa aagcagaaac agaatgaaat atagaatgcc agatagcaac acgaaatggg
     3001 aactgctgaa taaattgagt aaacagtata aaagctatac atgcctcgat gctaaatgta
     3061 tagatgacaa tccttgttaa aacatggcga acaggaagtt ttgatacatc gctatacatg
     3121 ttttgcaagg taaaacgcca tttaatggat atgctactac ctattaagga aagtaaagca
     3181 actgaaaatg tcattatgcc caaaccacct aactggataa gcaacaagat gactacctgt
     3241 ccaataaagg tgaaatcctt agcagtatct aaaaccacta agccagtaac gcaaactgct
     3301 gatgttgaag taaataatgc atcgataaat gtgatgccgt gagttgtgca aacaggaagg
     3361 cataaaagaa aagcaccgga aaatattacg ataacaaagc ttaaaaggac cttctcaatt
     3421 ggcgatagat attcttgtaa ctttttcata gcactataaa ttggggtata cgcctgtata
     3481 ccccaattac tgtgggcgct agaggatttg aacctctgac cctctgcttg taaggcagat
     3541 gctctcccag ctgagctaag cgcccctatt caatagcagt gtttttactg gacttaaagt
     3601 caataacata ctttgacggt actaccactt cccgctgagc aattggtgaa aacaccttgc
     3661 gctccgcctt tctgatcctt gtaaaaacgc caaacccctt aatagtaacc ttctcgccat
     3721 tttgaaccga ttgcactatt tcttccacaa aaccatcaac aatttgctgt actacatcac
     3781 gggggatttc atgcttaata tgcaatctgg taattatttc gtgcttggtc aaaaattacc
     3841 ctctgcgtca ttgtgataca gcattaacgc tattaacctt ctttgctaat cgtgactttt
     3901 tgcgtgctgc ggttttccag tgaagtatat ccttacctgc tgctgcatca atttttttaa
     3961 caaattcttt aaacaacacc tgtgcagttt catttgaatt gttttcaagg cttttgcgca
     4021 ctttcttttc agcagttctg acagatgatt taatcttaga atttttttgc cttctttttt
     4081 cgttttgtct attccgtttc tcagctgatt ttatattagc caatgtaaca ccctcatatg
     4141 taaaaagtat aatttaagat tatcaattat attgaagatt caatgaaaag gatatatgca
     4201 gattgtacca cattgtgtca agaatttaat aaaaattttc ataaaaaacc tgtagaaaac
     4261 ttaacatttc tttttgagaa tatattttgg accgtttaaa aaagacttat attattctac
     4321 ctttaaatga actattaagt agaagcttaa tcaaacaatc tatattttcc cctgaatgtg
     4381 cctctacttt ctgagtactt acaaaatatc gccatagaca ttgttacatg aaaacccaca
     4441 attactactt actgcag
//
LOCUS       DAOSOX010000191         4441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19704_length_4441_cov_6.034200, whole genome shotgun sequence.
ACCESSION   DAOSOX010000191 DAOSOX010000000
VERSION     DAOSOX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19704_length_4441_cov_6.034200"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLX22_10780"
     CDS             complement(<1..231)
                     /locus_tag="PLX22_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20421.1"
                     /translation="MKRHTMLIVITLILAGMLSCSSGDTATVTIDTGIRQQAQLSMLD
                     KLIAWITFSQAVKADPVPSVLDDITSISVTITA"
     gene            729..1352
                     /locus_tag="PLX22_10785"
     CDS             729..1352
                     /locus_tag="PLX22_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20422.1"
                     /translation="MSTKKIIITIVTITMLLSCNEQKSEKITNTQVQNTTNVIAHTSA
                     NNITAKNEAETTTEVPKKIVPVEKWEYGFGLNQSGKPVCFDVTKSKYNTFYRYIPDID
                     EITGDMVTRATITIRTNAIVVCNGKKEVKYSAGFERSYINNPCRYYKHKFEDNTNYIS
                     ADGINWDTLEIPNLKITNETSPLGEPMVLVYFKCKYFQGSYYIVFGE"
     gene            complement(1392..1583)
                     /locus_tag="PLX22_10790"
     CDS             complement(1392..1583)
                     /locus_tag="PLX22_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20423.1"
                     /translation="MGTITIDKNELKELVRANLIDISKDRNDIKEIMEDILLGRMIKE
                     GRTGKYCSKKEIVDFLDSI"
     gene            2108..2701
                     /locus_tag="PLX22_10795"
     CDS             2108..2701
                     /locus_tag="PLX22_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT20424.1"
                     /translation="MKRLHYLLPVFIVAFVFSFSGCGYNKLQMLEEEVFKAYGDLESA
                     LQRRADLIPNLVATVKGYAAHEKQTLQAVIEARAKATSVQLSPKDLSNPEAIAKFQAA
                     QGELSNALGRLMVVIERYPNLKADAHFTDLMHQLEGTENRINVARQRYNEAVKDFNYN
                     IRMFPYNLTNMILLHLERKEYFKAEEGAKAAPEVSFD"
     gene            2713..3372
                     /locus_tag="PLX22_10800"
     CDS             2713..3372
                     /locus_tag="PLX22_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT20425.1"
                     /translation="MKNLVAQYITQADAQQIISTVKKVEKKTSAEIVPMIVNASFHYP
                     LASVYAAVTLSLPVALVLTHYIAPLFYYSRDNMWIFIALFSLLFLLLKGTIARNIFLL
                     RLFLNQHEVEAEVKEAAITAFYKNGLYKTAEKNGVLIYISLLEKRVWILADEDAHKKI
                     EQEFWDSCVATIIDGIKKKQFVPVLCTVIETIGSKLSILYPYKSDDVDELKNIIIGDS
                     Q"
     gene            3369..4199
                     /locus_tag="PLX22_10805"
     CDS             3369..4199
                     /locus_tag="PLX22_10805"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOT20426.1"
                     /translation="MRWFATICITMFIVVSPGYSQDSATVSIPPLKGRVNDYAGILNE
                     QTESMLEAKLAQLEKEDSTQVVVATVPSIGDEDIESYSIRLAEKWKIGQKGLDNGVII
                     LVAYNNRKMRIEVGKGLEEKLTDLLAGRIIDYIMIPHFKNGDYDTGISQAVDAIIDIV
                     KGTFDASTLKKKKSVSDYATFFFIALCTLMSACSANKWTSGILGVFTFSIMGLIFSFG
                     LTALIVFTVIGFFAGFILSFVPIIGGKGGSSSGGFFSGGGGFSGGGGSFGGGGASGSW
                     "
BASE COUNT         1386 a          782 c          850 g         1423 t
ORIGIN      
        1 tgctgttatc gtaacactta ttgaagtaat atcgtctaag accgaaggta ctggatcagc
       61 ttttactgcc tgcgaaaagg taatccatgc aatgagctta tccagcatgg aaagctgtgc
      121 ctgctggcgt atgccggtat cgatggtgac ggtggctgta tcacccgagc tgcatgatag
      181 catccctgca agtattaatg taatgactat gagcatggta tgtcgtttca ttggtttttc
      241 ctccattagt ttagtgtaac ctcacccttc attctgcccg gtacagcttc cgctttgtgc
      301 aaagcggaag gttgcccggt attgccagag cacgattgct gagcgaaatg aaagcaaagg
      361 gttggatata atagtatgaa ctatcgccac aacattaagt taaaagagct gataaataaa
      421 tactatttgc ctatatatac cttataataa ttaaagaagt gtaacaatta tgccaattta
      481 tagcaatact ttttttagat ttttaaaaaa aagaacaata agtccatgcg gattttttta
      541 ttgtagcaac gccattcatt atgtctttaa ccgattgtac agtgtcttta cccataattt
      601 tttattaatt ttattacttg acatatcata gtatgttatc ctgactatag aggttctttg
      661 aaataaaggt gccaatcatt attatgctat atacgttcaa aatagtgctt tattgaaaga
      721 ggtacaacat gagtacaaaa aaaataatca taacgatagt taccattact atgttattgt
      781 catgtaatga gcaaaaatca gaaaagataa ccaataccca ggtgcagaat acaactaatg
      841 tcattgcaca taccagcgcc aataatatca ccgcaaagaa tgaagctgaa accactacgg
      901 aagttccaaa aaaaattgta ccggttgaaa agtgggaata tggatttggg ttaaaccaaa
      961 gcggcaagcc agtgtgcttt gatgttacta aatcaaagta taatacattt tatagatata
     1021 tacctgatat tgatgaaatt accggcgaca tggtaacaag agcaaccatt actatccgta
     1081 ctaatgcaat agtggtatgt aatggtaaaa aagaggttaa atattcagcg ggttttgaac
     1141 gctcgtatat caacaacccc tgcagatatt ataagcataa atttgaagac aacaccaatt
     1201 atatatccgc tgatggtatt aactgggata cattagaaat accaaattta aaaataacca
     1261 atgaaacaag cccactgggg gaacctatgg ttctggtata ttttaaatgt aaatattttc
     1321 agggttcata ctatattgtt tttggcgaat aatgactgat tttttgtaat ttttaaaatt
     1381 tccaatactg tttatattga atcaaggaag tccactattt cctttttgct gcaatatttt
     1441 cctgttctgc cttctttaat cattctaccc aataggatat cttccataat ttctttaata
     1501 tcatttctat cttttgatat atcaattaaa ttagcccgta ccagttcttt taattcattt
     1561 ttatcgatag ttattgttcc cataatcaca cctcatcaat gacatttata ccgctataat
     1621 acaattgcta ccaaatgtaa ataactcttc ttacgcttat cttacagtcg tttttatccc
     1681 ctctgcatct ttattgtatt aaatatatat aaaaatttta ttaactcaca taacaatcaa
     1741 ataggctctg accctatacg gtcttatctc attcatttta ttaaatgaaa atatcttctc
     1801 tataaaaaaa taaatgaact atccatgtca tgtgaagacg ccacgtatgg tgtttctaca
     1861 taattcatgg cgtccttacc attgctctta tttatttatt tattttatta cttgacatca
     1921 ttttttatat agtttatttt tatacaatct ttttttgaat aaatacatgg cagtaaccat
     1981 gccgtatggt atgttgtact gcacatgaat cagtgatagt aaataaacaa cataacatgg
     2041 taaaaacgtt gtttgccatg ttgttgtttc gggaatacta attgattgta attacaatgg
     2101 aggacacatg aagaggctac attacctgct ccctgttttt attgttgctt tcgttttttc
     2161 tttttccggg tgtggttata acaaacttca gatgctggaa gaagaggtat ttaaagcata
     2221 cggtgattta gaatcagcat tacagcgcag ggctgatctt attcccaatc tggttgctac
     2281 tgttaaaggc tatgcagcgc acgaaaaaca aacattgcag gcagtgatag aagcgcgggc
     2341 aaaagccact tcggtacaac tatcgccaaa ggatttaagc aatcccgaag ccattgcaaa
     2401 atttcaggct gctcagggtg agctttccaa tgcgctaggc cgtctcatgg tggttattga
     2461 acgctatccc aatcttaaag ccgatgccca tttcaccgac cttatgcatc agcttgaagg
     2521 caccgaaaac cgaatcaatg tggcacgcca gcgttacaac gaagcggtaa aagattttaa
     2581 ttataatatc agaatgtttc cctataatct tactaatatg attttacttc acctggaacg
     2641 caaagaatat tttaaagccg aagagggagc aaaagcggct cctgaagttt cttttgatta
     2701 gcgatagtta ctatgaaaaa tctggttgca caatatatta cacaggctga cgcacagcag
     2761 attataagca ctgtcaagaa ggtggaaaag aaaaccagcg ctgagattgt tcccatgatt
     2821 gttaatgcaa gtttccacta tccccttgca agcgtgtatg ctgctgtaac tttatcactt
     2881 cctgtagcgc ttgtgttaac tcattatata gcaccgcttt tttactatag cagggataat
     2941 atgtggattt ttattgcgtt gttttcactg ctgttccttt tattgaaagg aactatcgcc
     3001 cgaaatattt ttcttttgcg ccttttttta aatcagcatg aggtagaagc tgaggtaaaa
     3061 gaggctgcca tcacagcctt ttataaaaat ggtttatata aaacagccga aaaaaatggc
     3121 gtgcttattt atatctcatt gctggaaaag cgcgtctgga tattagccga tgaggatgca
     3181 cacaaaaaga ttgagcagga attctgggat agctgtgttg ccaccattat tgacggaata
     3241 aagaaaaaac agtttgtgcc tgtactatgt actgttatag aaacaattgg cagcaagctg
     3301 tcaatactgt atccatacaa aagtgatgat gtagatgagc ttaaaaatat aattattggt
     3361 gatagccaat gagatggttt gcaactatat gtataactat gtttatagtt gtttccccag
     3421 gctactcaca ggatagcgcc acggtttcaa taccaccgtt aaaaggccgg gttaatgact
     3481 atgcaggtat tctcaatgaa caaaccgaaa gtatgctgga agcaaagttg gctcaactgg
     3541 agaaggagga ttcaacgcag gttgttgtag cgacagttcc ttcaattggc gacgaagaca
     3601 ttgaatcata ttctatacgg ctggctgaaa aatggaaaat tggacagaag ggactggata
     3661 atggtgttat catcttagtt gcatacaata atcgtaaaat gcgcattgag gttggaaaag
     3721 ggcttgagga aaaattgacc gaccttctag caggcaggat tatagattac atcatgatac
     3781 cacatttcaa aaacggcgat tatgataccg gcatatcgca ggctgttgat gctatcattg
     3841 acattgtaaa aggcacattt gatgcaagca cattgaaaaa aaagaaatcg gtttctgact
     3901 atgcaacatt tttttttata gcgctttgta cattgatgag tgcttgctca gcaaataaat
     3961 ggacaagtgg tatattaggg gtatttacct tttccattat gggattaatt ttttcatttg
     4021 gattgactgc acttatagtg tttactgtta ttggtttttt tgccggattt attttatcat
     4081 tcgttcccat cattggtggc aaaggtggtt ctagtagcgg aggatttttt tctggtggtg
     4141 gaggcttcag cggaggcggc ggcagctttg gcggcggcgg tgcttcaggt agctggtaaa
     4201 ttgcaaaact cagaatagta ttcaaacaaa agttattata attatttttt gttagacagt
     4261 tcatacagta aaggacttgc aatgtattta ggacttgacg taggcggcac acatactgac
     4321 acagtaatta ttggcaattc aggtgttatt gcaaagtcaa agattgcgac cactcatgat
     4381 aatctgttaa gctgcataac agattctttt tccgcagttt tggccaatgt tgatccatcg
     4441 c
//
LOCUS       DAOSOX010000192         4429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19763_length_4429_cov_6.336077, whole genome shotgun sequence.
ACCESSION   DAOSOX010000192 DAOSOX010000000
VERSION     DAOSOX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19763_length_4429_cov_6.336077"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLX22_10810"
     CDS             <1..374
                     /locus_tag="PLX22_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20427.1"
                     /translation="FSTTPETTGLVCRDGSIFSIDDCEIFHASPPQCMVVNACQSASG
                     SLFLKNSFAYAVLKMGVATYIGTNFFLEAQRSFVFIKTFLKNITSNTTYYNAYASALQ
                     ELQKNFGNDDIALYNYVYYGN"
     gene            complement(420..2435)
                     /locus_tag="PLX22_10815"
     CDS             complement(420..2435)
                     /locus_tag="PLX22_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOT20428.1"
                     /translation="MSIIYIDESGAFDQYQKKRNVIGGIWCKMPVPHNEVKQLFSQCG
                     LSKVHACELDNETLSLLVQKLIPFCEGHSIKPVILLPRRGFFIISDTATYINVLSDGL
                     VEFIKTYAKELDATIEIIIEHRKGFKVDVDTKRLHEAIAKGVALSKVQNNINYTITFR
                     GKDNIFLQLADVVVHSFYRLDHDGSFLKGEVKEAFKEWMEPNTVYIYGNESYVTRIYD
                     CLRKGDYGEALQLYANHYMIPEVRQQKDFIIKNILKLSQPTMAVVLKNILTLYYDKVN
                     TNRLLNEYEPLVKLWIYTILPDIEKAIADGNNTSTAVDDLYWAYGELYQILLTIYNHK
                     GQIADFETTYNEARILLKKAVYDLDSLSVRIRIQVLNAVHYINQYQFEQAYTSMKVLA
                     KRVEDAFVFLSEADNDVKVKPRILGEIYGTMVQACMYHTLVTKNNFNEARELSNKALH
                     LFSHTDDIQRQYQYRAQIETYDGNYEQAMQYIAKGVGCTTYSDDDLIAQCLKNKSMFG
                     LQHLLRIWYCKLMNGYENTYYTVLSNINKPGSTIAKNYKEIMEHKVYPVHSIYRCVIV
                     IYGLLQSKHWEEHINKLLPRAKELFDGQPLTMQTLQLGLLADVLWIFAHHDAENKEIM
                     DRFNKRFNDVMHEASKYAIGDYLQKAYQHYQQTPVKKWNTLWWVFPF"
     gene            complement(2496..3062)
                     /locus_tag="PLX22_10820"
     CDS             complement(2496..3062)
                     /locus_tag="PLX22_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20429.1"
                     /translation="MGHLGNDLQCSSQMEELVLGVADGTITDNAIIDEVLEHLVHCNY
                     CYELYKDYFIIAQPLLTEQHGDDTLTLDIDYDNGFIPLNVNAQSFAAQNVVAVLSKQK
                     EPVIEFEIPLADGALTVRIIHVDGVFDIVVSNSNNSNGAKIFLLTDEKFEVATLSNNR
                     ATFTFIKPGIITLIYNFKKIITIKLNCK"
     gene            complement(3064..3699)
                     /locus_tag="PLX22_10825"
     CDS             complement(3064..3699)
                     /locus_tag="PLX22_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT20430.1"
                     /translation="MKKQRLYILIIMMINNMIGNKSKEELLKENFEYIDKTIRNVNNK
                     YNYNLTDEDREDILQQICLKLYETGFDNFQWQCKVTTYIFKIVENEIRSYVRKRNKII
                     VSTDLRKHDEQYNNNITEDFINNISYTIDFAQQLTNDEVAKIIKDTVDAMPALQQLIF
                     KLHFVDGIKQEDVAKMCKRSQATIAEHIGNIRKSIKQEVEKKFKGFIKELQ"
     gene            complement(3877..4158)
                     /locus_tag="PLX22_10830"
     CDS             complement(3877..4158)
                     /locus_tag="PLX22_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOT20431.1"
                     /translation="MIATQHFLQRAHNRGITNMELQLVQMYGYQKNDCIILDRKQTRD
                     MLDELNKLRRVLINIYEKGGISMVVQDDSLITTYPINRRINKKKAKYHV"
BASE COUNT         1299 a          857 c          637 g         1636 t
ORIGIN      
        1 tattcagcac aacacctgaa actaccggac ttgtatgcag ggatggaagt atattttcca
       61 ttgatgattg tgaaattttt catgcaagcc caccacaatg catggttgtt aatgcctgcc
      121 aatctgcaag cggcagttta tttttaaaaa attcgtttgc gtatgccgtg ctaaaaatgg
      181 gagttgcaac atacattggc acaaactttt ttctggaagc acaacgcagc tttgttttta
      241 taaaaacctt tttaaaaaat attaccagca acactaccta ttataacgcc tatgccagcg
      301 cactgcaaga attacaaaaa aactttggca acgatgatat tgcattatac aattatgttt
      361 attatggaaa ttaatcatac ctatgttatg ggcgatagtt actggcaatg ttcttttttt
      421 taaaaaggga aaacccacca taatgtattc cactttttta ccggtgtttg ctgatagtgc
      481 tgatatgcct tttgcagata atctccaatt gcatattttg atgcctcatg catcacatca
      541 ttaaatcgtt tgttaaatct atccatgatt tctttatttt ctgcatcatg gtgtgcaaat
      601 atccacaaaa catcagcaag caatcctaac tgtaatgttt gcattgttaa tggctgtcca
      661 tcaaataatt cttttgctct gggcaacagt ttattaatat gttcttccca atgttttgac
      721 tgcaataaac catatataac aataacacac cggtatatac tatgaacagg ataaaccttg
      781 tgttccatta tctctttata attttttgct attgtacttc ctggtttgtt tatatttgac
      841 aaaacggtat aataagtatt ttcataacca ttcataagtt tgcagtacca gatacgcaat
      901 aaatgttgca acccaaacat ggatttattt tttaaacatt gcgcaattaa atcatcgtcg
      961 ctgtatgttg tgcatcccac cccttttgca atatattgca tagcctgttc gtaattgcca
     1021 tcgtaagttt caatctgagc acgatactga tactgccgct gtatatcatc tgtatgagaa
     1081 aataaatgta gtgccttatt tgaaagctcc cgtgcctcat tgaaattgtt tttagtaaca
     1141 agcgtatggt acatacatgc ctgtaccatt gttccgtata tttcgccaag tatccgcggc
     1201 tttaccttaa catcattatc cgcttcagat aaaaagacaa acgcatcttc caccctttta
     1261 gctaatactt tcatgctggt atacgcctgc tcaaactgat actggttaat atagtgtaca
     1321 gcatttaaca cctgtattcg tatccgcacg gataatgaat caaggtcata tactgctttt
     1381 tttaacagta tccgtgcttc gttataggta gtttcaaaat cagctatctg ccctttatga
     1441 ttgtatatgg ttaacaaaat ttgatacagt tcaccatatg cccagtacaa atcatcaaca
     1501 gctgtgctgg tgttattacc atcagctatt gctttttcaa tatccggcaa tatcgtataa
     1561 atccacagtt taaccaatgg ctcatattca tttaacaaac ggttggtatt taccttatca
     1621 tagtataatg tcagtatgtt tttaagaact accgccatgg ttggctgact caatttcagt
     1681 atgtttttta ttataaaatc tttctgttgc cgtacttcag gaatcatgta atggtttgca
     1741 tagagctgta aggcttcacc ataatcgccc tttcgcaggc aatcataaat tcttgttaca
     1801 tacgattcat tgccataaat atatactgtg tttggttcca tccattcctt aaatgcttct
     1861 tttacctcac cttttaaaaa gctgccatca tggtcaagac ggtaaaagct gtgaaccacc
     1921 acatctgcca gttgcaaaaa aatattatct ttccctctaa atgttattgt gtagtttata
     1981 ttgttttgta ctttgctaag cgctactcct tttgcaattg cctcatggag cctttttgta
     2041 tcaacatcta ctttaaagcc ttttctgtgt tcaataatga tttcaatagt ggcatcaagt
     2101 tcttttgcat aagtttttat aaattcaacc aatccatcgc tcagcacatt aatataggtg
     2161 gctgtatcgc taataatgaa aaaacccctt cgtggaagca aaataacggg ctttatggaa
     2221 tgaccttcac aaaatggtat aagcttttga acaagcaatg aaagcgtctc gttatccagt
     2281 tcgcaggcat gaacttttga tagtccacat tgtgaaaata actgttttac ttcattgtgt
     2341 ggtactggca tcttgcacca tataccacca ataacattac gctttttttg atactggtca
     2401 aatgctccgc tttcatcaat ataaattatg ctcatactat cataccctgt tagttataca
     2461 acagcgtaaa gtataacaaa ttattattac atgttctatt tacagtttaa ttttatggtt
     2521 ataatttttt taaaattgta tattaaagta ataatacctg gctttataaa ggtaaatgtt
     2581 gctctgttat ttgacaatgt tgccacttca aatttttcat cagttaataa aaatatcttt
     2641 gcgccattac tattgttact atttgaaaca acaatatcaa aaacaccatc aacatgtatt
     2701 atccttacag tcagcgcacc atccgctaag ggtatttcaa actcaattac cggttccttt
     2761 tgttttgaca aaacagcaac aacattttgg gcagcaaagg attgagcatt tacattcagc
     2821 ggtataaaac cattatcata atcaatatca agggtaagtg tatcatcacc atgttgttcc
     2881 gtgagtaagg gttgtgcaat aataaaataa tctttatata gttcatagca atagttgcaa
     2941 tggactaaat gctccagcac ctcatcaata atggcattat cagtaattgt tccatcagca
     3001 acacccagta ccaattcttc catctggctg ctacattgca gatcattgcc taagtgtccc
     3061 atgttactgc aactccttta taaatccttt gaactttttt tccacttcct gtttaatgct
     3121 tttccgtata tttcctatat gctcagcgat agtagcctgt gaccgtttgc acatctttgc
     3181 gacatcttcc tgttttattc catctacaaa atgtaattta aaaataagct gttgtaaagc
     3241 aggcattgca tcaacggtat ccttaattat tttagctact tcatcattgg ttagttgctg
     3301 agcaaaatct atagtatagg agatattatt gataaaatct tctgtaatgt tattattata
     3361 ttgttcatca tgctttctta aatccgtaga tactattatt ttatttcttt ttcgtacata
     3421 ggatcgtatt tcattttcta ctattttaaa gatatatgtt gttaccttgc actgccattg
     3481 aaaattatca aaacctgttt catacagctt taaacatatc tgctgtaaaa tatcttccct
     3541 atcttcatct gtaagattat aattatattt attattaaca tttcttattg tcttgtctat
     3601 gtattcaaaa ttttctttta atagttcttc ttttgattta ttgccaatca tgttgtttat
     3661 catcattatt attaaaatat ataagcgctg ttttttcatg gtattacctt tatagtatta
     3721 gtcaataaca ttttaatatt tatataactc ctctatcatc ccatgcttga tttttcccac
     3781 agtttctgag tagttacagc atcacatcaa taatacagaa taattaaaaa aaagaggctt
     3841 gcaacagtgc attgttattg caagcctcat caaatattac acatgatatt ttgccttttt
     3901 cttgttaatg cgcctattga taggataggt agttattaag ctatcatcct gcaccaccat
     3961 tgaaatacca cccttttcat aaatatttat caataccctg cgcagcttgt ttagctcatc
     4021 cagcatatcc ctggtttgct ttctatccag gataatacag tcgttttttt gatacccgta
     4081 catctgcacc agttgaagct ccatatttgt aatacctctg ttatgcgcac gctgtaaaaa
     4141 atgttgtgtt gctatcatag agccctccct ctatataata gttagtgttt atagtattag
     4201 attactcaac ataaaattta taggtaattt tgttacacat tatacagtat cagcttcatt
     4261 ttgtacaatt tgcttatgat aacattacta ttatgtattt ttcttctctt tcgtttgtta
     4321 aagcgtcatg tgcatggcta ctaccttgct cttaacgtgt gattatactt tttgctcttt
     4381 ctttttactg ttttttattt ctctttttta cttcggccaa acgctttat
//
LOCUS       DAOSOX010000193         4420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_19810_length_4420_cov_10.476976, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000193 DAOSOX010000000
VERSION     DAOSOX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19810_length_4420_cov_10.476976"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLX22_10835"
     CDS             <1..770
                     /locus_tag="PLX22_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT20432.1"
                     /translation="IGYLRIKVFSEDTASEVKKAFQYFQKNNIPKCIVDLRWNPGGLL
                     DQAVAISDMFLPKDALIVSTKGRSGKETLQEYRSTTIPLYTGKCIILVNKGSASASEI
                     VSGALKDNGRAKLLGEKTFGKGSVQKFFNLDEDTGITLTIAKYYTPSGVSIHGIGIQP
                     DYTVTMATISREEQKQINILLKEKILQNFIDKHSKYDEATRKEFKDVLQKNNIKLSDD
                     TANFILKSEIYRFAKKPLYDLEFDNQLSEAMKIINVQ"
     gene            760..1755
                     /gene="tsaD"
                     /locus_tag="PLX22_10840"
     CDS             760..1755
                     /gene="tsaD"
                     /locus_tag="PLX22_10840"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT20433.1"
                     /translation="MFNKVLGIGLESSCDETAVAIVADGRKIVSHAIFSQIKLHQEYF
                     GVVPEIASRAHLEVINDCIDHCMKQANLSFHAIDYVAATCRPGLVGSLLIALQSAKAI
                     SYALSIPLIGVHHLEAHLYAPFLEGYGIEFPFIGLLVSGGNTALYLVHDFGKFELLGN
                     TTDDAVGEAFDKVAKFLLLGYPGGPIIETLAQQATVKKQLFPKILADSSESYSFSYSG
                     IKTAVIQYIHEHPDADIAEIAYSFQERALEILARRLFEASRRLKISTLVIAGGVASNK
                     RLREIIFEKKKEYHKIFIPSPILCTDNGAMVAGIGYQYFKKRMFSPLTIDVTARA"
     gene            1814..2560
                     /locus_tag="PLX22_10845"
     CDS             1814..2560
                     /locus_tag="PLX22_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20434.1"
                     /translation="METQTTYQNLIDAIIDIEEDAQYRDPGKAFVQLLSEYFFATETS
                     QNKAIAGFICQLPVPDQITKLPTLLKITPEDIKPFLETESINESLCARIMLQPAYLSF
                     AYPHHSPSFSKMPPEIKGEIIQSIKQRNQSILTAFEKLQQDIKATKERNIKALIALIL
                     KNVHLKTGMPFTKLSEPVSQLIEKNFNFCNEIFIASNKQISEINDDAKIKNILKSLFI
                     IKKFDELTEMSRVFKSEARRFIRRTQRILQ"
     gene            complement(2562..3764)
                     /locus_tag="PLX22_10850"
     CDS             complement(2562..3764)
                     /locus_tag="PLX22_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOT20435.1"
                     /translation="MKFNNFITSTIVPLYIVAIILFFAQNIFAVTLKWDIQPGSKLEF
                     VYDANVEYYENGMLRKAYSERNIIDLLCYNKETKGQFVKGTFSVYRKNINDYLYRLEE
                     QYSSDFFIRVNGMYDVPEGKFMPNLRHLPSFPEGDVKEGSVWQAPVNIIFNNFSVPLI
                     LLLEAQYIVLYINNESHEAIINYSILIDKTLKDKPYRDDMPIKIYGQYAGQVTWDFMQ
                     NQPKKSVNAYNLLLLFGRTGELNTIEFKMAIDQTNMAYKHIPDNEKKKQQKEIEKELK
                     HDGVSVDSDTRGMIIRFSDILFEFDKYNLNGEALKVLEKLVTIIKAKYPNNEVLVEGF
                     TDNVGSDDYNMILSEKRAQTVATYLKNSLGHDKISYRGLGKNNPIADNTTAEGRQKNR
                     RVEIIIKM"
     gene            complement(3754..4257)
                     /locus_tag="PLX22_10855"
     CDS             complement(3754..4257)
                     /locus_tag="PLX22_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20436.1"
                     /translation="MDSGDIGRQASVEGFAHEHIVVGLLMKKYQNVSLVDLPLSPYDI
                     IIVRKLINGSEDIIRAQVKTSKKSIIFTGGIRGGVDREYKSNVKTYIQSPETSDVIIG
                     LNPLKEGSFDMYFIPTVLIKRFGTKSKSIKSMELFKNNYEMLENCKNEEFIIQRCKDS
                     GFKLYEV"
BASE COUNT         1450 a          841 c          730 g         1399 t
ORIGIN      
        1 atattgggta tctcaggata aaggtcttta gcgaggatac cgcatccgaa gttaagaaag
       61 catttcaata tttccagaag aacaacatac caaaatgtat tgttgatttg cgatggaatc
      121 cgggaggact tctggatcag gctgttgcaa taagcgatat gtttttgcca aaggatgctc
      181 tcatagtatc aacaaaaggg cgtagtggca aggaaacatt gcaggaatac cgttccacaa
      241 caatacctct gtatactggc aaatgtatta tactggtcaa taaaggaagt gcttcagctt
      301 ctgaaattgt ttcaggtgct ttaaaggaca atgggcgggc aaagctattg ggcgaaaaaa
      361 catttgggaa aggctctgtt cagaaattct ttaatttaga cgaagacacc ggtataacac
      421 ttactatcgc aaagtactac acgccgtcag gtgtttccat acacggcata ggaatacagc
      481 ccgattacac cgttaccatg gcaactatct ccagagaaga gcagaagcag ataaatattt
      541 tactgaaaga aaagatttta caaaatttta ttgataaaca ttcaaaatat gatgaagcaa
      601 ccagaaaaga atttaaagat gttctgcaaa aaaacaatat aaagctgtct gatgatacag
      661 caaactttat attaaaatca gaaatctacc ggtttgcaaa aaagccgctg tatgatcttg
      721 aatttgataa tcaattatct gaggctatga aaattattaa tgttcaataa agtacttgga
      781 attgggcttg aaagttcatg cgatgagacg gctgttgcga tagttgctga tggaaggaag
      841 attgtatccc atgctatttt cagccagata aaacttcatc aagaatattt tggtgttgtg
      901 cctgagattg cctcacgtgc tcatcttgag gtaatcaacg attgtattga ccattgcatg
      961 aaacaggcaa acctatcatt ccatgctatt gattatgtgg ctgcaacctg ccgacctgga
     1021 ctggttggtt ctttgcttat tgcactccaa tctgcgaagg ctataagcta tgcactttcc
     1081 atcccactga ttggggttca tcatctcgaa gctcacctgt atgcaccatt ccttgaagga
     1141 tatggcattg agttcccatt tatagggttg ctggtttcag gagggaatac agcattgtat
     1201 ctggtacatg actttggcaa atttgagctt ttaggcaata ccaccgatga tgcggtaggt
     1261 gaagcattcg ataaggttgc aaaattttta ttactgggat accccggtgg ccccattatt
     1321 gaaacattag cccaacaggc taccgttaaa aaacaattat tccccaagat actggcagat
     1381 agttccgaat catattcatt ttcatacagc ggcattaaaa ccgctgttat acagtacata
     1441 catgaacatc ctgatgctga cattgcagag attgcctata gcttccagga acgcgccctg
     1501 gaaattctgg cacgcagact ctttgaagca tcccgacgat taaagatatc tacactggtt
     1561 atagccggag gtgttgccag taacaaacgc cttagagaaa ttatttttga aaaaaagaaa
     1621 gaatatcata aaatttttat cccatcacct attttgtgta ccgataatgg tgctatggtt
     1681 gcaggtatag gctaccagta tttcaaaaaa aggatgttta gccctttaac cattgatgtt
     1741 acggcacgag cttaaaaaaa tattgattat ttcttagttt cattatacta tattggcata
     1801 tattaacaac actatggaaa cacaaacaac atatcaaaat cttattgatg caatcataga
     1861 catcgaagaa gatgctcagt accgtgatcc tggcaaagcc tttgtacaac ttttatcaga
     1921 atattttttt gcaaccgaga catcccagaa taaagcaatt gctggcttta tatgccagtt
     1981 gcctgttcca gatcaaatca caaagctgcc aaccttacta aaaataaccc ctgaggatat
     2041 aaaacctttt cttgaaacag aaagtatcaa tgaatcacta tgcgcacgga ttatgctgca
     2101 acctgcatat cttagttttg catatcctca ccatagccca tcattttcta aaatgcctcc
     2161 agagataaaa ggcgagatta ttcaatcaat caaacaaaga aatcaatcta tacttactgc
     2221 ttttgaaaag ttgcagcagg atataaaagc tacaaaagaa cgaaacatca aagctcttat
     2281 agcattaata ttaaaaaatg ttcatcttaa aacaggaatg ccatttacaa aactatctga
     2341 acctgtatcg caactcattg aaaaaaactt taacttctgt aatgaaattt ttattgcttc
     2401 caacaaacag atatctgaaa taaacgatga tgcaaagata aaaaatatat tgaaatccct
     2461 gtttattatc aaaaagtttg acgaattaac agagatgtcc agggtattta aatccgaagc
     2521 caggcgcttt attagaagaa cacaaagaat tttacaataa tttacatctt aataataatc
     2581 tcaacccttc tgtttttttg tctgccctcg gcagtggtat tatcagcgat aggattattt
     2641 ttacccaatc cccggtatga tatcttgtca tgtcccagac tatttttaag atatgtcgca
     2701 accgtttgtg cacgtttttc ggaaagaatc atattgtagt catcactacc gacattatcg
     2761 gtaaaacctt ccaccagaac ttcattattg gggtatttag cctttatgat tgtcaccaat
     2821 ttctcaagta cctttaacgc ttcaccatta agattatatt tatcgaattc aaacaatata
     2881 tcagaaaatc gtataatcat cccgcgcgtg tcagaatcaa cggaaacgcc atcgtgtttt
     2941 aattcctttt caatttcttt ttgttgtttt ttcttttcat tgtctggaat atgtttatat
     3001 gccatattgg tttgatcaat tgccatttta aactcaatag tatttaactc accagtacga
     3061 ccaaaaagta acaaaagatt ataggcatta acagatttct taggctggtt ttgcataaaa
     3121 tcccaggtca cctgacctgc atactgacca tatattttta ttggcatatc atcacggtat
     3181 ggtttgtctt ttaaggtttt atcaattaaa attgaataat tgataattgc ttcgtgactt
     3241 tcattattta tatacagcac aatgtattga gcttcaagca acagaataag cggaactgaa
     3301 aaattattaa atataatatt aaccggtgct tgccacacac tcccttcttt tacatcaccc
     3361 tccgggaaag aaggcaaatg ccgtaaattg ggcataaatt tcccttctgg aacatcatac
     3421 atgccattaa cacgtataaa aaaatcagag ctatactgct cttccaaacg gtagagatag
     3481 tcattaatat tttttctata taccgaaaat gtccccttaa caaactgacc ttttgtttct
     3541 ttattatagc ataaaagatc aataatattg cgttcactgt atgccttccg tagcatacca
     3601 ttttcatagt attccacatt ggcatcatat acaaattcta attttgaccc gggctgtata
     3661 tcccatttca aggttaccgc aaagatattt tgtgcaaaaa ataatataat agcaacaata
     3721 tatagtggta ctatagtaga agtaataaaa ttattaaact tcatataatt taaaccctga
     3781 atctttacat cgctgaataa taaattcttc atttttacaa ttttcaagca tttcataatt
     3841 gtttttaaat aattccattg atttaattga tttactttta gtaccaaatc tctttatgag
     3901 aactgttgga ataaaataca tatcaaaaga accttctttt aaaggattta acccaataat
     3961 tacgtctgat gtttcaggtg attgaatata tgtttttaca tttgatttat attcccgatc
     4021 aacacctcct cttattcccc cggtaaaaat tatacttttt tttgaagttt taacctgtgc
     4081 tctaattata tcttcagatc cattgatgag ttttctgaca ataataatat cataagggga
     4141 tagtggcaaa tcaaccaatg atacattttg atattttttc atcaacaagc caacaactat
     4201 atgttcatgt gcaaaacctt caacagaagc ttgccgtcct atatcaccac tatccatgct
     4261 aattatttcc tcaatatcaa tatccagtca gtgcttattc tttcttccct tggtactttt
     4321 ataaaatgtt taattatttc tgaagcaaat tacaaggtcg ttttccggcg aacgtcatcc
     4381 acgacgggtc ggacgaggtg acggggctgt ttccgatgac
//
LOCUS       DAOSOX010000194         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_20193_length_4354_cov_9.073505, whole genome shotgun sequence.
ACCESSION   DAOSOX010000194 DAOSOX010000000
VERSION     DAOSOX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20193_length_4354_cov_9.073505"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLX22_10860"
     CDS             complement(<1..718)
                     /locus_tag="PLX22_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20437.1"
                     /translation="MKKPKKKSLLKYFEPVESGKSLSENFGSLDLEGSFGYFALSAQN
                     KSLFLNRLSVDLLYEMMKKVGLIDHLKKIGFYNIKLEIDKDDAQIHYLKIYYDTIDPD
                     HLLIDLRVSESRFIPEKRFCEEYNAPMFMDMVVIEWLSAQNPKSDFNTSKPQLPGQSR
                     PGLGSLNYMMELMYIVGEQVVKDGFMDVPDHFHGAVMYAKKFKFFNPSHEAILRAILR
                     DIKGYSLYDMSWGMITGTIID"
     gene            complement(741..1571)
                     /locus_tag="PLX22_10865"
     CDS             complement(741..1571)
                     /locus_tag="PLX22_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20438.1"
                     /translation="MVFENNSGCVCRLLESYNVKGKKYQESDQIYLDDINLFARAGID
                     TIDIKYDEIIYSYFSTLYPAEFRIPYSSADFFAFDRKLETLERISTLTKRKRYLICVG
                     DIYAYDQHTGKRIIVFRHNDSIEYKQWNQVKRLVDHSKKLYYRNSENGIIIFVNLQPH
                     ADSSYIERFKKNADLVSAIVSRKKDCKIEISPDFLPTEDVYTINNPNDLLSFYQKSNA
                     RLIIIGESLNDDYRKALIKVRDYDKFARMMVVPNIDLRNVDHFLLQVKMVYNSDRWTE
                     "
     gene            complement(1730..2812)
                     /locus_tag="PLX22_10870"
     CDS             complement(1730..2812)
                     /locus_tag="PLX22_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20439.1"
                     /translation="METLEFNEEVQKLRERTFNKEFRDLVAKVITKPASSTIDTPEKL
                     LEHFHQLYYIVLSKSFNDSVQFVDVESYIEDNSRHHGETVSSKLMSLGFAENLILQYD
                     FINTYFGILTNSVKGNFFSEFYLLPSDAIYNDVITQSEGVVISYKSIRDDQLYSKRLS
                     YVPDHDFYVWIFSTCHLVKKVISVLNITIPTVLELWCPIIMVKVYEKFSITSQLLNEI
                     YYATIIPIMTLLESYIIMNKSSLLLSSNYFHEVYKVFENYFYISKSENYTKYFILKSK
                     CENQLIYCLFRYLSQKYKDKKSFSTINFRPMIGLIIMFLTDEDEKLVSEAKNLVSILN
                     DYLEIAIIDKYMIKNAVQLYRYIIET"
     assembly_gap    2918..3017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3018..3319)
                     /gene="nadA"
                     /locus_tag="PLX22_10875"
     CDS             complement(<3018..3319)
                     /gene="nadA"
                     /locus_tag="PLX22_10875"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT20440.1"
                     /translation="MNMNLEESIARLKKEKKALIIAHNYQNPDVQDIADYTGDSLELS
                     RIAAKNDADIIIFCGVYFMAESACILSPQKKVILPDSSAGCPMADMADVDSILEMK"
BASE COUNT         1470 a          683 c          553 g         1548 t
ORIGIN      
        1 tatctattat agttcctgta atcatccccc atgacatatc atataatgaa taacctttta
       61 tatctctgag tatagctcgt aaaattgctt catgacttgg attaaagaat ttaaattttt
      121 ttgcatacat cactgcacca tgaaaatgat ccggcacatc cataaaccca tcttttacca
      181 cctgttcacc aacaatatac atcaattcca tcatatagtt taaactaccc aaacccggcc
      241 tggattgtcc aggaagttga ggtttggaag tattaaaatc tgattttgga ttttgtgctg
      301 atagccattc aattaccacc atatccataa acataggtgc gttatactct tcacaaaatc
      361 ttttttcagg aataaatctt gattcagaaa cccttaaatc tatgagcaaa tgatctggat
      421 ctattgtatc ataatatatt ttcaaatagt gtatttgagc atcatcttta tcaatttcaa
      481 gcttaatgtt gtaaaaccca atttttttca ggtgatcaat caatccaact tttttcatca
      541 tttcatagag aagatcaaca gataatctat ttaaaaacaa acttttattc tgagcactta
      601 gtgcaaaata gccaaaactt ccttccaaat caagcgatcc aaaattttcg gataatgatt
      661 ttccagattc aacaggttca aaatatttta atagtgattt ttttttaggt tttttcacaa
      721 attattcctt cttttcttaa ttattcagtc catcggtcag aattatagac catttttacc
      781 tgtaataaaa aatgatcaac atttctcaga tcaatattgg gcacaaccat catccttgca
      841 aatttatcat aatccctaac ctttatcaat gcttttctat aatcatcatt taaagattca
      901 ccaataataa taagacgcgc attcgacttt tgataaaagg acagaagatc gttaggatta
      961 ttaatggtat aaacatcttc tgtaggtaaa aaatctggtg atatctcaat tttacaatct
     1021 ttttttctgg atactatcgc actaaccaga tccgcatttt ttttaaaacg ttcaatatag
     1081 cttgaatctg catgaggttg taaattaaca aaaataatta tgccattctc tgagtttcta
     1141 taatacaatt ttttactatg atcaaccagt ctttttacct gattccattg tttgtattca
     1201 atgctatcat tatgccgaaa cactataatt ctttttcctg tatgttgatc atatgcatag
     1261 atatcaccaa cacatatcaa atatcgttta cgttttgtaa gcgtacttat tcgttctaat
     1321 gtttctaatt tcctatcaaa agcaaaaaaa tcagctgaag aataagggat gcgaaactct
     1381 gctggatata gtgtagaaaa atagctgtaa attatttcat catatttaat atcaatggta
     1441 tcaatcccag cccgggcaaa aagattaata tcatcaagat aaatttgatc tgattcttga
     1501 tatttttttc cttttacgtt atagctttcc agcagtctac aaacgcatcc tgaattattt
     1561 tcaaaaacca tttttaattg atcaattttt accaacatta tataataaaa aattttactt
     1621 ttatttatta tatcatcggc acacattata tactattaaa agaaatataa aaacaatatg
     1681 gttttttatt caagtacatt ttgaaatatc aatataataa ttaatttgtt taagtttcaa
     1741 taatatatct atataattga actgcatttt tgatcatata tttatctatt attgcaatct
     1801 ccaagtaatc atttaatatt gaaacaagat ttttagcttc actcacaagt ttttcatctt
     1861 catcagttaa aaacataatt attaaaccaa tcatagggcg gaaattaatt gttgaaaaag
     1921 attttttatc tttatatttt tgtgataaat atctgaaaag acaataaatt aactgatttt
     1981 cacattttga cttcaatata aaatattttg tatagttttc tgattttgat atataaaaat
     2041 aattttcaaa aactttatac acttcatgaa aataatttga tgataaaagc aatgaacttt
     2101 tattcataat tatataagat tcaagcaaag tcattattgg aataatagtt gcataataaa
     2161 tttcattcaa taattgggat gttatgctaa acttttcata aaccttaacc ataattatag
     2221 gacaccacaa ttcaagtaca gtggggatgg taatatttaa aaccgatata acctttttaa
     2281 ccagatggca tgttgaaaaa atccatacat aaaaatcatg atctggtaca tagctaagtc
     2341 tttttgaata taactgatca tctcttatag atttatatga tattacaacg ccttcagatt
     2401 gtgtgattac atcattataa atagcatctg aaggtaataa ataaaattct gaaaaaaagt
     2461 ttcccttaac agaatttgtt aaaataccaa aataagtgtt tataaaatcg tactgcagaa
     2521 taagattttc agcaaaaccc agggacatta attttgaaga cactgtctca ccatgatgcc
     2581 gggaattatc ttcaatatat gattctacat caacaaactg tacagaatcg ttaaaagatt
     2641 tacttaaaac aatgtaatat aattggtgaa aatgttccaa taacttttca ggtgtatcaa
     2701 ttgtacttga tgctggtttt gtaataactt tagctaccag atctctaaat tctttgttaa
     2761 atgttctttc tcgtaatttt tgtacttctt cattaaattc taatgtttcc aaagcacgcc
     2821 tcttccacat ttcaggataa ttctaacata cgttgcagcg attgtaaagc ttttaccctg
     2881 atatcttcct gcactgatac tatcggttct tcattaannn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnttt ttcatttcaa gaatagaatc aacatctgcc atatcagcca
     3061 ttggacaacc tgcactgcta tcaggtaaaa taactttttt ttgtggtgac agtatgcagg
     3121 cagactcggc catgaaatat acgccacaga atataatgat atcggcatca ttttttgctg
     3181 ctatgcgcga tagctccagt gaatcaccag tataatcagc aatatcctga acatcaggat
     3241 tttgataatt atgcgctatg attagtgctt tcttttcctt ttttaatcgg gctatcgatt
     3301 cttcaaggtt catatttata ttccttttga atgtaattta taatggcaat tttgaacatt
     3361 aaaacacatt gtctatttta tgcaattata ttattgcctt ttgaaaaagg tagtttaatg
     3421 aatattgaaa ataatataga cggcagcata gtaatagtat tttttaaaga acatttcgag
     3481 gcgatagttg ttgatgaaaa cacatgaact tatagcagat agtgtatatg ccaatagcgc
     3541 aattattaaa aacctgctga ggaagataac agtatcgcaa caacaatacg agtagtaagc
     3601 tatacagtga tatggttcgc catataattt atatagtata tagccccacc agagatgtaa
     3661 atgaaaaatc tattgagcgc tacgaccata caaagatgat aaaacgatta agatatagaa
     3721 gcccacgtga gacattacaa gaatattaca ataagagctt aaaagtgccc gataaatatg
     3781 aaccagaaca tacaataagc ttgagaatgg caattttttt gctgataatg ctgtaaaaca
     3841 ttcataataa tagatgtatc attaatgtta aaatgtactg caaaatacag ggtagatgtt
     3901 gtgcagtaaa gaagtaattt tattatataa ggttagcagt ccttttgatg attgagattt
     3961 taatacattg atggacaatt aaagcaaata atggaaggta atacgcaagt tttttggcaa
     4021 aaatttgata aaaattaaat tcaaaaaaag tagttttcta ttccatagtt agatattaaa
     4081 ggtcatttta atttaaactc aaaaacagtc gattctgagg ttacaaaaaa gtgctgctaa
     4141 tcattgatta attatttttt tacacaataa aaaaagccat acttataaag catggctttt
     4201 ttcccataca attcatcttg ttgttattac tttgcagttt tttcttcttt cttctgttct
     4261 ttagcttctt tcttctgttc tttagcttct ttcttctgtt ctttagcttc tttcttctgt
     4321 tcttgcttct gtgctttttc ttctttcttt actt
//
LOCUS       DAOSOX010000195         4349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_20220_length_4349_cov_7.674197, whole genome shotgun sequence.
ACCESSION   DAOSOX010000195 DAOSOX010000000
VERSION     DAOSOX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20220_length_4349_cov_7.674197"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLX22_10880"
     CDS             <1..760
                     /locus_tag="PLX22_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOT20441.1"
                     /translation="MDYEFIKSRLHLYAVLQNIEDLVQYDDVAKNLIKDWNIIVKFSV
                     RKAGSVYLIFENGKCTVRELSPKKPDVILFFTSPAHLNKMFDGKANPIPLKGITKLGF
                     LTKKFPKLTERLEYYLKPDEQKLKDKNYVTINTVMTLNTALYALKELLLFDPIGEHAA
                     KKVKGSMVMMNVNKGPAAYIEQKGNTFEVHKGYAQQPLAVLEFNSIKIANDFLNGKMD
                     AFSAIASGAVATKGHIEWLDAVSPVLDRVALYLS"
     gene            783..>1273
                     /locus_tag="PLX22_10885"
     CDS             783..>1273
                     /locus_tag="PLX22_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT20442.1"
                     /translation="MKVEAKGIKYSVSSKSKSKTYTYTKSQKLFQRALKVIPCGIPGH
                     FSPAPFVPPTMYPFYAERSKGSHFWDIDGNEFIDYMCAYGPIVLGYNHPQVEKAAQEQ
                     NSKGNCMTAPGKIMVDLAEYLTDTIAMADWAFFAKNGGDVTNLAVMIARAATGRKKIV
                     MIKG"
     assembly_gap    1274..1373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1374..1820
                     /locus_tag="PLX22_10890"
     CDS             <1374..1820
                     /locus_tag="PLX22_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOT20443.1"
                     /translation="HPISALVGVDALKTVAAKVFYTGSYWYQAAPMAASLATLKELRR
                     INAPVLLNKIGKKLFEGMKKICASYGYELKITGAYSMPFVRITNDPSNMLHQEWCAEC
                     TARGAYFTSHHNWFVCAAVSDKDIQKTWNIVDDVCKLLKKKYGDKY"
     gene            1915..2757
                     /locus_tag="PLX22_10895"
     CDS             1915..2757
                     /locus_tag="PLX22_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT20444.1"
                     /translation="MPLTQQEIQQFTAKACQLRKDAIDTVYWAGGGHIGGSLSFTEIM
                     ILLYYKYARIDPKNPGWDDRDRIVVSKGHAGVGYAPILADKGYFPRELLKEFNHTGSI
                     LGMHLDGNKVPGVDVSTGSLGHGLPITVGLGIGARLQGKSWLTYCVLGDGECDEGSVW
                     EAAMAAAHYKVTNIITIVDRNRMMIDGPTEEVMRLEPFADKWKAFGWIVKEVDGHDFN
                     QLADAIEYAQKEDKAPVVIIAHTIKGKCVDFMEDKYEWHYGGLDSEKVELAKACIDKH
                     YCEL"
     gene            2774..3787
                     /locus_tag="PLX22_10900"
     CDS             2774..3787
                     /locus_tag="PLX22_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT20445.1"
                     /translation="MAADGLTYTIVDTANMSTAEIYGKVLCDLAEKHKEIVGLSADLA
                     KSTKMGQFAATFPDRFFNAGIAEQNMFGMAAGLAKAGLTPFVSTFAVFASLRAAEQVR
                     TDICYQKLNVKIIATHAGTSFGQAGTTHHCTEDLAVMRSFANMVVIAPADGIETANAV
                     MAAYEHKGPVYIRINRGFDPFLYEKTDYGFQIGKAVQLKDGTDITIIACGAAAYNAYH
                     AANILEKEDKLSVRVINMHTIKPIDEEAILKAVMETRRIVTFEDHNIIGGLGSAVAEV
                     IVKSGKGCAFETVGIPDQYAIIGLPEDLQNYYGFDQNKIIEKVRQIMGKDFEQTEDWE
                     DEV"
     gene            3790..>4349
                     /locus_tag="PLX22_10905"
     CDS             3790..>4349
                     /locus_tag="PLX22_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20446.1"
                     /translation="MATQNELFASTIFLKAALPLTKVILEYRPKMAAKYKGLNGVVQF
                     IAKDEAGNIGAFLQFSDGVLDVKYGIHEHPDIVFTFKSVKAMNDFFAGKPAVPSIKGL
                     TKLKLLIKSVNLLLALKIIGRKTPPKKPEDRELMVRLLLYLLPLGISQLNKAGYPDIA
                     KFCQKSPDRTFAWSVTGTDIASYLRM"
BASE COUNT         1346 a          657 c         1009 g         1237 t
ORIGIN      
        1 tatggattat gaatttataa aatcacgatt gcatctgtat gcagtgttgc aaaatatcga
       61 ggatttggtg cagtatgatg atgttgcaaa gaatctcatt aaggactgga atatcatcgt
      121 aaagtttagt gtaaggaaag caggttcagt gtatctcatt tttgaaaacg gaaaatgtac
      181 tgtcagggaa ctatcgccaa aaaaacctga cgtcatcttg ttttttactt cacctgctca
      241 tttaaacaag atgtttgatg gaaaagcaaa tccaattccg ttaaagggaa ttacaaaact
      301 tggtttttta accaaaaaat ttcccaaact taccgagcgg cttgaatatt acttaaaacc
      361 tgatgaacaa aaacttaaag ataaaaatta tgttactata aatacggtaa tgactttgaa
      421 taccgcattg tatgcgctca aagaattgtt attgtttgac cctattggcg aacatgcagc
      481 aaaaaaagtg aagggtagca tggtgatgat gaatgtcaac aaagggcctg ctgcctatat
      541 tgagcaaaag ggcaatacct ttgaggtgca taagggatat gcacagcaac cattagctgt
      601 actggagttt aattcaataa aaattgccaa tgatttttta aatggaaaga tggatgcatt
      661 cagtgccatt gcttcaggtg ctgtggctac aaaaggtcat attgagtggc ttgatgcagt
      721 cagtccggtg cttgataggg tagctttgta tttatcataa cctgtaacag tgaggtaaaa
      781 gtatgaaagt agaagcaaaa ggaattaagt atagcgtttc atcaaaatca aaaagcaaaa
      841 catatacgta cacaaagtct cagaagctat ttcagcgcgc acttaaggtt ataccgtgcg
      901 ggattccggg acatttcagt cctgcgccgt ttgtgccgcc aacgatgtat cctttttatg
      961 ctgaaaggtc caaaggttca catttctggg atattgatgg gaatgaattt atagattata
     1021 tgtgtgcgta tggacccatt gttttagggt acaatcatcc tcaggtagag aaagctgctc
     1081 aggagcaaaa tagtaaaggt aattgcatga cagctccggg taaaattatg gtagatttag
     1141 ctgaatatct tactgacacc attgcgatgg ctgattgggc tttctttgca aagaatggtg
     1201 gtgatgttac caatttagca gtgatgattg ctcgggcagc tacaggaaga aaaaaaattg
     1261 ttatgataaa gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncatccta
     1381 tttctgcact ggttggtgtt gatgcattga agactgttgc agcaaaggtg ttttatacgg
     1441 gcagttactg gtatcaggca gcaccaatgg cagcatctct tgccacatta aaagaattac
     1501 ggcgcatcaa tgctccagtt cttttaaata aaattggcaa gaagctcttt gaaggtatga
     1561 aaaagatttg cgcatcatat ggatatgaat taaaaataac gggtgcatat tcaatgccct
     1621 ttgtaaggat taccaatgat cccagtaata tgttacatca ggagtggtgt gctgaatgta
     1681 ctgcaagggg tgcgtatttt acctctcatc ataattggtt tgtttgtgcg gcagtatctg
     1741 ataaggatat acagaaaaca tggaacatcg tagacgatgt gtgcaaactc ttaaagaaga
     1801 aatatggtga taaatattaa ttatgcttgc ttttgtattg aaggcaacca gaatagtata
     1861 aggaactatc gaacataaaa catacatgtg aaattatttt acaggaggag atatatgcca
     1921 ttaacacagc aggaaataca acaatttaca gccaaagcct gtcagttacg aaaagatgct
     1981 attgatacag tgtattgggc tggaggtgga catattggcg gttcgttaag ttttaccgaa
     2041 attatgatct tattgtatta taaatatgca cgaattgatc caaagaatcc gggatgggat
     2101 gaccgcgaca gaatagtggt aagtaaaggc catgctggag ttgggtatgc gccaatatta
     2161 gctgataaag gttactttcc cagagagctg ttaaaagaat ttaatcatac tggatctatt
     2221 ttaggaatgc atctggatgg caacaaggtt cctggcgtag atgtttcaac gggttcactg
     2281 ggtcatggac tgcccatcac tgttgggttg gggattggtg cgcgacttca gggtaaatca
     2341 tggttaacat actgtgtgct gggtgatggt gaatgtgatg aaggttcggt atgggaagct
     2401 gctatggctg ctgctcatta taaggtaacg aatattataa caattgttga tagaaacaga
     2461 atgatgattg atggtcctac cgaagaggtg atgcgccttg aaccgtttgc cgataaatgg
     2521 aaagcatttg gatggatagt gaaagaggtt gatgggcatg attttaatca actggctgat
     2581 gctatagaat atgcacaaaa agaggataag gctcctgttg tcattattgc tcataccatt
     2641 aagggcaagt gtgttgattt tatggaagat aaatacgagt ggcattatgg cgggttggac
     2701 agtgaaaagg ttgagcttgc caaagcatgt atagacaaac actactgcga actataatgt
     2761 aaggaggatt attatggctg ctgatggatt aacatatact attgttgaca ctgccaatat
     2821 gtcaacggca gaaatttatg gtaaggtttt atgcgattta gctgaaaaac ataaggagat
     2881 tgtagggctt tctgcagacc tggctaaatc caccaagatg ggacagtttg ctgcaacctt
     2941 ccctgacaga ttttttaatg ctggtattgc cgaacaaaat atgtttggca tggctgcagg
     3001 gcttgcaaaa gcagggctta cgccatttgt ttcaacgttt gcggtttttg cttcattgcg
     3061 ggcagcagag caggtgcgaa ctgatatatg ctatcaaaaa cttaatgtca agataattgc
     3121 tactcacgcg ggaacttcgt ttggtcaggc tggtactact catcattgca cagaggatct
     3181 ggcggtaatg cgttcttttg ccaacatggt ggtgatagct cctgcagatg gaattgaaac
     3241 tgctaatgcg gtaatggcgg cgtatgagca taagggccct gtgtatatac gtatcaatag
     3301 aggatttgat ccatttttat atgaaaaaac tgattatggc ttccagattg gcaaagcagt
     3361 acagcttaaa gatggtactg atattactat tattgcgtgt ggtgctgcag cgtataatgc
     3421 gtatcatgct gccaatatac tggaaaaaga ggataaatta agtgtacgcg ttatcaatat
     3481 gcataccata aagccaattg atgaagaggc tattttaaaa gctgttatgg aaacacggcg
     3541 tatagttacc tttgaagacc ataatataat aggtggactg ggaagtgcag tagctgaggt
     3601 aatagtgaaa agcggaaagg gatgtgcctt tgaaacagtg ggtatacctg atcagtatgc
     3661 aataatagga ctgcccgaag atttgcagaa ctactacggg tttgatcaga ataagattat
     3721 tgaaaaagta cgtcagataa tgggtaagga ttttgaacaa actgaagact gggaagatga
     3781 ggtgtgatta tggcaaccca gaatgaattg tttgcttcaa cgatattttt aaaagctgca
     3841 ttaccactta ccaaagtgat tctggaatac cggccaaaaa tggctgcaaa gtataaaggg
     3901 cttaatggtg ttgttcaatt tatagctaaa gatgaagcag gtaatattgg tgcttttttg
     3961 caatttagtg atggtgtact ggatgtaaaa tatggtatac atgaacatcc tgacattgtt
     4021 tttaccttta aaagcgtaaa agcaatgaat gacttctttg caggtaagcc agcagtgcca
     4081 tctattaagg gattaactaa attaaaactt ttgataaagt cagtaaattt actgttagcg
     4141 ctaaagatta ttggaagaaa aacccctcca aagaaaccgg aagatagaga gcttatggta
     4201 aggttattgt tatatctgtt accgttgggt atcagccagc ttaataaagc tggatacccg
     4261 gatatagcaa aattttgtca aaaaagcccg gatagaactt ttgcatggtc ggtgacgggt
     4321 actgatatag cttcgtattt gcgcatgaa
//
LOCUS       DAOSOX010000196         4335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_20321_length_4335_cov_6.846028, whole genome shotgun sequence.
ACCESSION   DAOSOX010000196 DAOSOX010000000
VERSION     DAOSOX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20321_length_4335_cov_6.846028"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLX22_10910"
     CDS             complement(<1..857)
                     /locus_tag="PLX22_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOT20447.1"
                     /translation="MQPLSGNHKQQTIIYHCLHNALLPHSLLFTGLEGIGKKLFALTL
                     AKMMFCKNNACNTCPSCLQIEHGIHPDVIVVEPNDKGTIPIGNNDEHEGSIRWLIARI
                     SLAPVNGNRVVIINDAHTMNVQAQNALLKTIEEPPRNNFIILISSERNQLLPTIHSRI
                     TEYVFNPLSQEELTAILVHNGVPHEKHNAILPVASGSASYALTLSDDTLYAMIVDAYR
                     AIVSFVVHPDVFFHDLLPLLQAIGPYKTIDLLINIFRLHCSNSFPSLPWLDSYLKEET
                     AVEIIKILL"
     gene            complement(863..1303)
                     /locus_tag="PLX22_10915"
     CDS             complement(863..1303)
                     /locus_tag="PLX22_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HOT20448.1"
                     /translation="MIEIVPPNQKKNEQKLVKQKKGSKTAGASSPFRTTFEQTIEFSL
                     PETIEELLNNLKDQEKNFLSDPSMYNMQRYKALIQKIVKILVSEEYTVNVLQRKRRDR
                     ADFIVIQKIENKLEAITNAYIKNEAFNFLKTFDEIRGLILDLKH"
     gene            complement(1300..1662)
                     /locus_tag="PLX22_10920"
     CDS             complement(1300..1662)
                     /locus_tag="PLX22_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT20449.1"
                     /translation="MARTNKFNVEDNIVNSIIGEGSEFKGEFKINGLLRIDGKFKGTI
                     ATDGKVLIGQTGEAITDIKARVVVIGGTIKGNIFASERVIMLSTGVIQGNVITPSLIM
                     EDGVTFEGNCIINKEQNI"
     gene            2896..2967
                     /locus_tag="PLX22_10925"
     tRNA            2896..2967
                     /locus_tag="PLX22_10925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2929..2931,aa:Arg,seq:ccg)
     gene            3108..4019
                     /locus_tag="PLX22_10930"
     CDS             3108..4019
                     /locus_tag="PLX22_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOT20450.1"
                     /translation="MTNLTHIIEKYFLVWIVVFSLCGYHFPALFKGLAAYIPLFLGII
                     MFGMGMTLEFTDFIHIAKIPGALIAGVIGQFIIMPFVAYLLCLVFQLPPMLAIGVILV
                     GTCPGGTASNVVTYLSKGDVALSVALTITTTLVSPLATPLLTYLYAGTWIHVPVIPML
                     VSILQVIIIPIILGITIRSVFKEKIKTVITILPSVSVVAIVTIVAGITAGNALALKTV
                     GLLVIGVVMVHNVLGLIMGYLASWLFGFDEKIRRTIAIEVGMQNSGLAVSLAVIHFEA
                     ISALPGALFSIWHNISGSLIAWWWRRH"
BASE COUNT         1276 a          752 c          828 g         1479 t
ORIGIN      
        1 tcaagtaata ttttaataat ttccacagcg gtttcctctt taaggtaact atcgagccag
       61 ggaagggaag ggaaactatt gctgcaatga agtctgaaga tgttaattaa aagatcaatt
      121 gttttataag gacctatggc ctgtagcagt ggcaaaagat catggaaaaa aacatcaggg
      181 tgtacaacaa agcttacaat ggcacgatag gcgtctacta tcatagcata tagtgtgtcg
      241 tcagaaagtg tcagagcata ggatgctgaa ccgcttgcaa cgggaaggat ggcgttgtgt
      301 ttttcatgag gcacgccatt gtgtactaga atggctgtaa gctcttcttg cgaaagaggg
      361 ttaaagacat attcagtaat ccgggagtgt atggttggca atagctgatt gcgctcagag
      421 cttattaaaa tgataaagtt attgcgaggt ggttcttcaa tggttttaag aagggcattt
      481 tgcgcctgca cattcatggt atgagcatca ttaatgataa caactctatt gccattaacc
      541 ggcgcaagcg aaatgcgtgc aataagccag cgtatagaac cttcatgttc atcattatta
      601 ccaatcggta tggtgccttt atcattgggt tcaactacaa taacgtcagg gtgtattcca
      661 tgttcaatct gaagacatga ggggcatgta ttacacgcat tgtttttgca aaacatcatt
      721 ttggcaagtg tcagagcaaa caattttttg ccaatacctt caagcccggt aaaaagcagg
      781 gaatggggaa gcaaagcatt gtgtaaacag tgataaataa tagtttgttg cttatggttg
      841 cctgataatg gttgcatggt aatcaatgtt taagatccag gataagtcct ctgatttcat
      901 caaaggtttt taaaaaatta aatgcctcat tttttatata tgcatttgtt attgcttcaa
      961 gcttgttctc aattttctgg ataacaataa aatctgctct gtctcgacgc ttgcgctgta
     1021 aaacattaac tgtatattct tcgctcacca gtatttttac tatcttttgt atcagtgctt
     1081 tatatcgctg catattatac attgaaggat cggataggaa atttttttcc tggtctttca
     1141 ggttgtttaa aagttcttct atagtttcgg gtaaagaaaa ctcaatggtt tgttcaaagg
     1201 tagtacggaa gggagatgat gcccccgctg ttttactccc ttttttttgt ttaacaagct
     1261 tttgctcgtt tttcttttga tttggcggta ctatctcgat catatatttt gctctttatt
     1321 aatgatacaa ttaccttcaa aggtaacacc atcttccatt ataagactgg gagtaataac
     1381 gttaccctgt attacaccgg tagaaagcat aataacacgc tcagaggcaa agatattgcc
     1441 ttttatagtt ccaccaataa cgacaacacg agctttgata tctgtgatag cttctcccgt
     1501 ttgtcctata agtactttcc catcggtggc gatagttcct ttaaatttac catcaatacg
     1561 gagcaaccca ttgattttaa attcaccttt aaattcagag ccttcaccga ttatgctatt
     1621 tacaatatta tcttcaacat taaatttatt tgttcgtgcc atatgaactt cttatttaag
     1681 attttgtgct gtatgcaatt atgttattat tttattaata tggtacagta caaaattata
     1741 cctataaaga caagtataat tttatgacat ttttgctata tacatgagga tcaaaaaatt
     1801 ttgacaaata taaaaaaaag cttgcaaatt ttataaaagg ttatattata tccagaacgg
     1861 aatagtatgc ttaaattcaa ggagggaacc atgaggaaaa caatcatcaa acttttggaa
     1921 tatggttggc ccgaccgaat tatcgcaagc taccttatga taaagcctgc cattgttaag
     1981 tactatagga aaaaatatag aataacccgt aacgagtatc aggggtttta ctgatataat
     2041 gtcagatctt tacataataa cactataaaa aagtagaggg gaagagggtt cccctctaaa
     2101 gagaatttgg aggttcgcca atcattaaat tggaggaaag tatagcacca aaaaaatttt
     2161 gctgcttact tgtcaacact attaatagct tatatcggca aaaaaaacaa aaaattttgt
     2221 attaatcctt taaaaaattc aaaagattgt gtttatataa cctgtaatgc aaaaattttt
     2281 tattttgtat agttatatat ttatattttg aagttatgga atcgatatgg tacatgaaaa
     2341 atggatcttt tagttttaat ccattgtaat tatctccatt ccttgttacg agagttacgt
     2401 catatatctg tgctgttgca gctatgatag tatcagcaat ctttactttt tttcgttttc
     2461 tattttttaa agcctggatc ggtacccttt gagcttaaaa gctccctttt ttttcctatt
     2521 cctgaagcaa tatttgattt tactgctata ccattttctg gagaatcaat cagaaataat
     2581 agtatttcga tattcttgtt tatatagtca tcaggtactt ttattgtata attatttttt
     2641 gctggtttta taaaaaaaca tagattttac tatctccata tttttttaag agctactcgt
     2701 attttaaatg tctacagtgt atactattga aaacacaatg attatttgta tctataaaca
     2761 gtatgccaat cattgtatta tttcttgttt gtgttggtaa tactactgtt gctattgcct
     2821 cagcattgca tggtattata aattatcttg acaggtaacc gtgtctttta ctaaatagaa
     2881 acggtttggt atacagctct cctagctcaa cggatagagc gtcggcctcc ggagccgtgt
     2941 gtccgggttc aattcccggg gagagcgagt tttttacctg ctctgtacat tccatgctgg
     3001 tgtaaccact acgcagttca ttacaccttt gcaattatta aatgctataa attgttaaac
     3061 agtaactatc gccccatacc ttttttataa gtaaccggaa cacatatatg acaaacctaa
     3121 cgcatatcat tgaaaaatat tttttagtgt ggattgtggt tttttcacta tgtggctatc
     3181 atttccctgc tctattcaaa gggcttgcgg catatatacc gttgttttta ggtattatca
     3241 tgtttgggat gggcatgaca cttgaattta ctgattttat acacatagca aaaattcctg
     3301 gtgcacttat tgcgggagtg ataggtcagt ttattatcat gccatttgtt gcatacctgt
     3361 tgtgcctggt atttcaattg cctccgatgc ttgcaattgg ggttatactc gttggcacat
     3421 gccccggcgg cacagcatcc aatgttgtca cctacctgtc aaaaggcgat gtggcacttt
     3481 cggttgcact gacaattaca acaacacttg tttcaccgtt agctacaccg ctgttaacct
     3541 acctgtatgc gggtacatgg atacatgtgc cggttatacc catgctggtt tccatactgc
     3601 aggtcattat tataccaatt attctgggaa tcaccatccg ctccgtgttc aaagaaaaaa
     3661 taaaaactgt cataaccata ctcccctctg tttcggttgt ggcgatagtt actattgtag
     3721 cgggtattac tgccggaaat gctttagccc tgaaaacagt tgggctgctt gtgataggcg
     3781 ttgttatggt gcacaatgtg ctgggactga ttatgggtta cctggcaagc tggttatttg
     3841 gctttgatga aaagataagg cgaaccattg ccattgaggt gggtatgcaa aattccggac
     3901 ttgcggtgtc cctcgcggtt atccattttg aagcaatcag tgcgctgcca ggtgcactat
     3961 tcagtatatg gcacaacatt tcgggctcgc tcattgcgtg gtggtggaga cgacattagt
     4021 tcatattaca cataattgct gtaattttgt ctgttaacga ggtgctgatg agttatagaa
     4081 aattattata agccagactg aggtaaaaaa gcacatctct actttatcgg tgcgttggat
     4141 atacaaaaat cagcatatgt tattaattgt ctctgtaata acaaaaattg aaaatatcgc
     4201 tttcgaaggt cttttaatgg cgaatgctat taatgaaaat gattatcata ttttatgcaa
     4261 tgaatttgag attgattatc tatagagtac ttattcacct aatgttttct gcaagttgtc
     4321 aacatgaccg gtgag
//
LOCUS       DAOSOX010000197         4319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_20418_length_4319_cov_9.909944, whole genome shotgun sequence.
ACCESSION   DAOSOX010000197 DAOSOX010000000
VERSION     DAOSOX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20418_length_4319_cov_9.909944"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLX22_10935"
     CDS             <1..605
                     /locus_tag="PLX22_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20451.1"
                     /translation="LSMHDETLIPFFYDIALLPDVDSAISAIVGLSLYRNKFANWEKL
                     YTGEKDYDAMVAIVSSTNINEYNLCAYNQNKYMLFLYCRWAEMFNAKKGDILGLMNIT
                     LHTLPENHILFTRTVEIIGHILCTLKNHEFNHFNDNDIDELIMIFNSLPPEKVDGIIR
                     FWGIPKSNFIHTLQQTINKKQIRLDNCSNIATLLCTTEFE"
     gene            723..>1330
                     /locus_tag="PLX22_10940"
     CDS             723..>1330
                     /locus_tag="PLX22_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT20452.1"
                     /translation="MVLVTGCNSLIGQTLIKRLITDRYQVKAIDMWKDAKLPDEVTFL
                     QGNLLDTDFIDEAMSDVDIIFHLMDVEDASYYGRRFMKKINIKSTENLLRAAAENNIK
                     HFIFLSSGKVYGNPDVMPIKEDDPCKPNTAYGKDKVKAEKLCRKFAEDGLPVTIIRPT
                     IITGPGVDDAMILIILYMAMAMGDSNRLYIAGDGDSRYQLVHP"
     assembly_gap    1331..1430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1431..2701)
                     /locus_tag="PLX22_10945"
     CDS             complement(<1431..2701)
                     /locus_tag="PLX22_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20453.1"
                     /translation="MPQIIANIQMPSRPSLDFFDYWDSLGLFDELSVLLPPEFFIIFE
                     IAQVIFTPELISEALNAVEDISREEKIEAPNRDESILINRVERISPLSDIMVIDRYRT
                     IYDLKKALPRELAWEDTIFDIKLFKHELLVQKFYEEKADTFKPISTLQDESGRQANRF
                     DQKFYLLLDRSRSMEIRMRSFFSKCLVAEFLRRKLNSNAKIFYRPFDSKPGDLFKIEK
                     KEDFPFLIERVLLTTTGGTSTNLQQAVFQAIDDINFDKDMLNSEILVVTDGISKIDKY
                     LLKEKLGDIKLNILKVGRDLAEPDYFEMKKTFDANKMKFDPTALNIKEIQHKMDKSQE
                     EEALSPLQKRAYRYILEYSETMFKDLKEVSHRFVEIPDLDPSSLYELTDEKLDAIETA
                     VDDFSRIDFNTKTLEERKKLFKQAYFLSQYVT"
     gene            complement(2707..4176)
                     /locus_tag="PLX22_10950"
     CDS             complement(2707..4176)
                     /locus_tag="PLX22_10950"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20454.1"
                     /translation="MKSQNPSHRNYLSDAESLLDQEGYIIIAPDEDKKELQPEDKPTT
                     KTEHVVTQQVEKLPGKKPEIVTKTIEAEATDIPSTIQYLKWHMERIAKNVINREEIIK
                     QAMYAILTREHMLLLSRTGMAKSYLANYIFNTFDNVRVFASQASKEQTPDNYFGPYNI
                     EEFKKGRIRHNIHGSIIEANLVFLDEFFDASDVVLRSLLSVLNERKFINGPEIIDAAI
                     HTAIATANYMRMNEVTEAILDRFTYKSIIPEDDNVYQQLLIDHTYAFSRGKAIEPDKK
                     IPFNQIIYLSDIVRNKSKEVKIEIPDFVYFMKNVITNKFVSDMRKSDYKFFVSPRKQA
                     KLQDFLRANALLNNRFEVTMDDLKEMYLASCTLNSFISVKAKDKSEKDVFLDAYQQTM
                     VHFNATGAIQQIEFLLNLRRIFQEIMENPEKKERLLASSSGILRGLRNLLKKIFPSRS
                     SDEEITLETLKRSLTDLQPAVEEVRELKEGITKDYFAIL"
BASE COUNT         1293 a          717 c          769 g         1440 t
ORIGIN      
        1 tgttatcgat gcatgatgaa acactcatac cctttttcta tgatatagca ctgcttcctg
       61 atgttgatag tgcaatcagt gcaattgttg gattatcact gtataggaat aagtttgcta
      121 attgggaaaa attatataca ggtgaaaagg attacgatgc aatggttgca atagtatcat
      181 caactaatat taatgaatac aacttgtgtg catataacca gaataaatat atgttatttt
      241 tatattgccg ttgggccgaa atgtttaatg caaaaaaagg tgatattctg ggtctcatga
      301 atataacatt acacacatta cctgaaaacc atatactttt cacaagaaca gttgaaatta
      361 ttgggcatat actctgtact ttaaaaaatc atgaatttaa tcattttaat gataatgata
      421 ttgatgagct aattatgatt tttaatagcc ttccacctga aaaagttgat ggcataattc
      481 gtttctgggg tattccaaaa agtaatttta tacatactct tcagcaaact atcaataaaa
      541 aacaaataag attagacaat tgttcaaata tagcaacttt attatgcaca accgagtttg
      601 aatagtagcc atgcaatctg taatggctct atatttaatg ctatcatcgc tattttacgc
      661 ttgacgaaat caataatcaa aataaaatgc ttgggcatgt agatcatcct atggagggta
      721 ccatggttct ggttacagga tgtaacagtt tgattggcca aactcttata aaacggctca
      781 ttactgatag gtatcaggtt aaggccatag atatgtggaa ggatgcaaaa ttacctgatg
      841 aggtgacatt tctacagggt aacttgcttg atactgattt tattgatgaa gctatgagcg
      901 atgtggatat aatattccat ttaatggatg ttgaagatgc ttcgtactat ggtcgtagat
      961 tcatgaaaaa aataaatata aaaagtacag aaaatctttt aagggctgct gctgaaaata
     1021 atataaaaca ttttattttt ttatcaagtg ggaaggtata tggtaatcct gacgttatgc
     1081 caattaaaga agatgatcct tgcaaaccca acacggcgta tggtaaggac aaggtgaagg
     1141 ctgagaagtt gtgcagaaag tttgctgaag atggtttgcc ggtaactatt atacgcccaa
     1201 caataattac cgggcccggt gtagacgatg caatgatcct tattatttta tatatggcaa
     1261 tggcaatggg tgattcaaac agattatata ttgccggtga cggtgattca cgatatcaac
     1321 ttgtacatcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtcacgtact
     1441 gagataagaa ataagcttgc ttaaaaagtt ttttgcgttc ttccagtgtt ttggtattaa
     1501 aatctattcg agagaaatca tctactgctg tttcaatggc atctaacttt tcatcagtaa
     1561 gctcatataa tgaagaagga tctaaatcgg gaatttcaac aaaacgatgc gatacttctt
     1621 ttaaatcctt aaacatcgtt tctgaatatt caagtatata tcggtaagcc cgtttttgta
     1681 atggcgataa tgcttcttct tcttgagatt tatccatttt gtgttgtatc tctttaatat
     1741 taagagcagt aggatcaaac ttcattttgt tagcatcaaa tgtttttttc atttcaaaat
     1801 aatcaggctc agccagatca cggccaacct tcaagatatt aagtttaata tcgcccagct
     1861 tctcctttaa taaatatttg tcaattttgc tgataccatc ggtaacaacc agtatttctg
     1921 aattcagcat atctttatca aaattaatgt catcgatggc ttgaaacaca gcttgctgca
     1981 agttggtgct tgtcccaccg gttgttgtaa gtaaaaccct ttcaattaaa aaagggaagt
     2041 cttctttttt ttctatttta aaaaggtcac ctggttttga atcaaaagga cggtaaaata
     2101 tttttgcatt gctgttaagc ttgcgtcgta aaaattctgc cacaagacat tttgaaaaaa
     2161 acgatcgcat cctgatttcc atggatcgtg aacggtcaag taatagataa aatttttgat
     2221 caaatctgtt tgcttgtctt ccactttcat cctgtagcgt ggatattggt ttaaatgtat
     2281 cagctttctc ttcgtaaaat ttttgtacaa gtaattcatg cttaaaaagc ttgatatcaa
     2341 aaattgtatc ttcccacgct aattccctgg ggagcgcttt ttttaaatcg taaatggttc
     2401 ggtaacggtc aatgaccata atatcactta atggcgatat gcgctcaacg cgatttatta
     2461 aaatagactc atcgcgattg ggagcttcaa ttttttcttc acgggatata tcttcaacgg
     2521 cattgagcgc ttctgatatc aattcgggag taaatataac ctgagctatc tcaaagataa
     2581 taaaaaattc aggtggtaat aataccgata gctcatcaaa taaacctaat gaatcccagt
     2641 agtcaaaaaa gtctaaactt ggtcgtgatg gcatctgaat gtttgcaatt atctgtggca
     2701 tagcttttat aatattgcaa aataatcttt tgttattcct tctttaagtt cgcgaacttc
     2761 ctcaacggcg ggctgtaaat cggttaagct tcgctttaag gtctcgagcg taatctcctc
     2821 atcagatgat cgtgatggaa atattttctt taaaagattt cgtaagcctc tgagtatacc
     2881 tgaggacgaa gcaagcagac gttctttttt ttcagggttt tccatgattt cctgaaaaat
     2941 tcgtctaagg ttcagtaaaa actcaatttg ctgtatagcc cccgtggcat taaaatgaac
     3001 catggtctgt tgatatgcat caaggaatac atccttttct gatttatcct ttgctttaac
     3061 agaaataaag ctattcagcg tacacgatgc taaatacatc tcttttaaat catccatggt
     3121 aacctcaaaa cggttattta acaaagcatt tgctcgtaaa aaatcctgca actttgcctg
     3181 ttttcggggg cttacaaaaa acttataatc tgattttcgc atatcgctta caaatttatt
     3241 ggtaataaca tttttcataa aatatacaaa atcagggatt tcaattttta cctctttact
     3301 tttattgcga acaatatctg agagataaat aatctgatta aatggaattt ttttatcagg
     3361 ttctatcgct ttaccgcgac taaacgcata agtgtggtca ataagaagtt gttgatatac
     3421 attgtcatct tcgggaatta tggatttata ggtaaaacgg tctaaaatag cttccgttac
     3481 ttcattcatg cgcatgtagt ttgctgttgc aatagcggtg tggatggctg catcaattat
     3541 ttcagggcca tttatgaatt tacgctcatt cagaactgat aaaagagagc gtaatacaac
     3601 atcacttgca tcaaaaaatt catccagaaa aacaaggttg gcttcaataa ttgatccgtg
     3661 aatattatgc cgtatacgcc cttttttaaa ttcttcaata ttgtaagggc caaaatagtt
     3721 atcaggtgtt tgttctttac ttgcttgtga ggcaaaaact ctgacattat caaacgtgtt
     3781 gaagatataa tttgcaagat atgattttgc cataccggtg cgtgataata acagcatatg
     3841 ctcgcgtgtt aagatagcgt acatagcctg ttttattatt tcttccctgt tgatgacatt
     3901 ctttgcaatg cgttccatat gccatttgag atactgaata gtgctgggaa tatcagtagc
     3961 ttctgcttca attgtttttg ttacaatttc aggtttttta ccgggcagtt tttcaacctg
     4021 ctgagttact acatgctctg ttttagttgt gggtttatcc tcgggctgca attccttttt
     4081 atcttcgtca ggcgcgatga tgatatatcc ttcctgatca aggagtgatt ctgcatcact
     4141 gaggtaattt cgatgagaag ggttttgtga tttcatgtgg tccccctatt agtcaatagc
     4201 tggaagtaaa attttaaatg atgttccttc accaggggcg ctttttaccc ctattgctcc
     4261 gccgtggttg tttataatgg tataggtaac catcagtccc aatccagttg atttgggtc
//
LOCUS       DAOSOX010000198         4313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_20456_length_4313_cov_9.332081, whole genome shotgun sequence.
ACCESSION   DAOSOX010000198 DAOSOX010000000
VERSION     DAOSOX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20456_length_4313_cov_9.332081"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLX22_10955"
     CDS             <1..233
                     /locus_tag="PLX22_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20455.1"
                     /translation="LWYIAFNPVRKRRCDSPVKYRYSSIQSYVQEGADVGVPIDHHEY
                     FLQLGDSFSERVRRFLQYEEAYRKRYSIGQWV"
     gene            complement(242..1660)
                     /locus_tag="PLX22_10960"
     CDS             complement(242..1660)
                     /locus_tag="PLX22_10960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT20456.1"
                     /translation="MATEKKSPNQELQEKLSVKKESAWLLLNDKVNDIYDFAEEYKQF
                     IGKNKTERLCVRSMVEMLKQKKYKDIQTVSKLLKGDAVYKNIKGKSLIACKIGSDPTK
                     IRIIGAHIDSPRLDLKPYPLYEDAEVALLKSHYYGGIKKYQWVNIPLAIHGVAFTKDG
                     HRVEIHIGENHDEPRFIIPDLLPHLAKDQMKKTGENIIEGEELNILFAHIPIDDKDIK
                     EKIKLNVLQYLHTTYGLVEEDFIAADIELVPAMQPVDIGIDKGLIAGYGQDDRACAYT
                     VWKALVDAGNCKDTVIMYLADREEIGSMGDTGAESFVFIHFMREYIRKTGITSTWDEL
                     LEGALSISADVTGALDPTFKNVNDTNNVSYLGHGVSIEKYGGRGGKYSTSEAHAEYMQ
                     YLRSLADKHKIPWQTGEQGKIDMGGGGTIAMFMSRYGMNCVDVGPCMLGMHSPYEVTS
                     KVDIYCAYRIYKAFFEDDTFKL"
     gene            1955..2311
                     /locus_tag="PLX22_10965"
     CDS             1955..2311
                     /locus_tag="PLX22_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT20457.1"
                     /translation="MVKPIDLEGRVVHDPILKKTKTGKSVCTFAIVLDYPTKGETKPY
                     PSYIDIETWDKLADLCAEHIKKGRLVQATGRLKQDRWQGEDGKARSKIKLVTKIVKFI
                     DPITSSKNNEEVVLQP"
     gene            complement(2342..2500)
                     /locus_tag="PLX22_10970"
     CDS             complement(2342..2500)
                     /locus_tag="PLX22_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT20458.1"
                     /translation="MVEYITLTVLLVLSLAYSIRYIIRAIVTVNECNCDNCAIDCERK
                     RRPQKRLV"
     gene            complement(2539..4269)
                     /locus_tag="PLX22_10975"
     CDS             complement(2539..4269)
                     /locus_tag="PLX22_10975"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT20459.1"
                     /translation="MKMSRYLVPTLKEDPSDAVITSHRLMLRAGLIRKESAGMYIYLP
                     LGLRVLQKIITIIREEMNKSGALEFLMPELTSADLWKESGRWHSMGPEMIRIKDRNGI
                     EYALAPTHEEAFTNVIRMVVASYKDLPVNAYQINTKFRDEIRPRFGVMRSKEFIMKDA
                     YSFDSDEAGLEDSYQIMRQTYRTIFKRCGLETIPVEADTGSMGGSNSEEFMVASEVGE
                     EVLLLCEVCGYKSNQERATCQYQLSPTNEDEEALAIVDTPDVRTINELVQFFGCSAHT
                     FLKSLIYVADGTPIMAVVPGDREINEVKLKNTLNAVDLELAPDAVVEEVTGAPVGFAG
                     PILVKGKALRIIFDTQIKTIKNAITGANQRDKHYKGVNPGRDFVIGQEADIVLVVAGD
                     RCPHCGNALIETRGIEVGHIFKLGYKYTRAMNVKVLDKNGKAITPIMGCYGIGVNRTM
                     AAVIEQHNDDRGIIWPMSVAPFQVHCISLAKNEKEEGAADTIYSMLVDAGIEVLYDDR
                     TASPGFKFGDADLIGIPIRITLGKAFFGDSSSIEVKLRNKDEKIIIAKDMIVDYCKKQ
                     IVQMLQKLNA"
BASE COUNT         1262 a          998 c          738 g         1315 t
ORIGIN      
        1 tgctgtggta catagcgttc aatccggtac ggaagcgccg gtgcgatagt cctgtcaagt
       61 accgctacag cagcatacaa tcctatgtgc aggagggtgc tgatgttggt gtaccgatag
      121 atcatcatga gtactttctg cagttagggg acagtttcag tgaacgagtg agacgttttt
      181 tacagtatga ggaagcatac cgcaagcgct actccattgg acagtgggtg taaaaaaggg
      241 gtcagagctt gaaagtatca tcttcaaaga aagctttata gatacgatag gcgcagtaaa
      301 tatccacctt tgaggtaacc tcatacggcg aatgcatccc taacatacat ggacctacat
      361 caacacaatt catgccatat cgactcataa acattgctat ggttcctcca ccacccatgt
      421 cgatcttacc ctgttcacct gtttgccatg gaattttatg tttatcagcc aatgagcgca
      481 aatactgcat atattctgca tgtgcttcac tggtggaata ctttccaccc ctaccaccat
      541 atttttcaat gctaacccca tgccccaaat acgatacatt atttgtatca ttgacatttt
      601 taaaagtagg atcaagtgca ccagtaacat ctgctgaaat cgaaagcgct ccttcaagca
      661 attcatccca tgttgacgtt atacccgttt ttcgtatata ttcacgcata aaatgtataa
      721 acacaaaact ttctgctcct gtatcaccca tagaacctat ctcttcccta tctgccaaat
      781 acattataac tgtatctttg caattaccag catctactaa agctttccat acagtataag
      841 cacaggccct atcatcctga ccataccctg caataagccc tttatcaatg ccaatatcta
      901 caggctgcat agcaggtaca agttcaatat cagcagcaat aaaatcctct tctaccagtc
      961 cataagtggt atggaggtat tgcaatacat tcaatttaat cttttccttt atgtccttat
     1021 catcaatcgg gatatgcgca aatagaatat taagttcttc accttctatt atattttcac
     1081 cagtcttttt catctgatct tttgcaaggt gtggtagtaa atcaggaata atgaatcgcg
     1141 gttcatcatg attttcgcca atatggattt caacacgatg accatctttt gtaaatgcaa
     1201 caccgtgtat tgccagtggt atattaaccc actggtactt ttttatccct ccgtaataat
     1261 gtgacttcaa aagtgcaacc tccgcatctt catataatgg gtagggtttt aagtctaatc
     1321 taggtgaatc aatatgcgcg ccaataattc tgatttttgt ggggtcagag cctattttgc
     1381 aggcaattaa acttttccct tttatgtttt tataaacggc atcacctttt agtagtttgc
     1441 taaccgtttg gatatcctta tatttttttt gcttcagcat ttctaccatt gaccgcacgc
     1501 acagtcgctc agttttattt ttcccaatga attgtttata ctcttccgca aaatcatata
     1561 tatcattaac cttatcgttc aataatagcc atgcactctc tttttttaca ctcaactttt
     1621 cctgcaattc ctgatttggt gatttcttct ctgttgccat actatcttca cccttattaa
     1681 tgtaatttat ttaatcaaag atacgtattt cataattcct tcactatact atatcacatg
     1741 catctaataa acacaatcct ttcatggcat ttttataggg aataaaccag cattaaggtt
     1801 actcacatag aaaattgggg tcagagctta ataaacttaa aattttttta aaaaaattaa
     1861 aaaatttttg cccaaagtgt taatttttcc aatctccggc gttttatata atgaaaagtt
     1921 ctttatcaaa atttatagta aacgaggtgt tactatggtt aaaccaatcg atcttgaggg
     1981 aagagttgtt catgacccaa tcttaaaaaa aacaaaaaca ggaaaatcag tatgcacatt
     2041 tgccattgtg cttgattatc caacaaaagg cgaaacaaaa ccttatccat cctacattga
     2101 tattgaaaca tgggacaagc ttgccgacct ctgtgctgaa catatcaaaa aaggcaggct
     2161 tgtccaggca acagggcggt taaaacagga ccgatggcag ggtgaggatg gtaaagctcg
     2221 ctccaagatt aagcttgtca ctaaaatagt aaaattcatt gaccctataa caagcagcaa
     2281 aaataatgaa gaggttgtgc ttcagccata agtctgtggt tgctcttccc taaaaattat
     2341 tttaaacaag gcgcttttgg gggcgccttt ttctttcaca atcaatagca caattgtcac
     2401 agttacattc attgacagta actatcgccc tgataatata acgaatgcta taagcaagcg
     2461 atagcaccag taaaaccgtt agtgtaatat attccaccat agtatcaagt atagcactac
     2521 taacgccggg aaacaaggtt atgcgttgag tttctgtaac atctggacaa tctgcttttt
     2581 acagtaatcc acaatcatat cttttgcaat gattattttt tcgtctttat tccgcagctt
     2641 aacctctatc gaggaactat cgccaaaaaa tgcttttccc agcgtaatgc gaataggaat
     2701 gcctattaga tccgcatcgc caaatttaaa gccagggctt gccgtgcgat catcatacaa
     2761 aacctcaatg cccgcatcaa caagcatcga atagattgtg tcagccgcac cctcttcttt
     2821 ttcattcttt gctaaactga tgcaatgtac ctgaaacgga gcaacactca ttggccatat
     2881 aatacctctg tcgtcattgt gctgttcaat gactgctgcc atagtacggt taacacctat
     2941 cccataacat cccataatag gcgtgattgc cttaccgttt ttatcaagaa ctttcacatt
     3001 cattgctctg gtatatttat atcccaattt gaaaatatgg ccaacctcaa tccctcttgt
     3061 ttcaataagc gcattgccac aatggggaca cctatcacca gccacaacca gtactatatc
     3121 agcttcctgt ccaataacaa aatcacgtcc tggattaaca cctttgtaat gcttatccct
     3181 ctggtttgcg ccggtaatgg catttttgat agtctttatc tgagtatcaa aaattattct
     3241 taaggctttc cccttcacaa gaatgggacc ggcaaaacct acaggcgccc cggtcacctc
     3301 ctcaactaca gcgtcaggtg caagttctaa gtcaacagca ttcagtgtat ttttaagttt
     3361 cacctcgttt atctctctat cgcccggcac cactgccata ataggggtgc catctgcaac
     3421 atagattaaa ctttttaaaa atgtatgagc gctacaacca aaaaactgta caagctcatt
     3481 gatggtgcgc acatcagggg tatcgacaat agctaacgct tcttcatctt cgtttgtggg
     3541 cgatagttga tactgacagg ttgcccgctc ctgattggat ttatagccac acacctcaca
     3601 cagcagcagt acctcctcac caacctctga tgccaccata aattcctctg agttactgcc
     3661 acccattgaa ccggtatcgg cttcaaccgg gattgtttcc agaccgcatc gtttaaagat
     3721 ggtacgatag gtttgccgca taatctgata tgaatcttcc aatccagctt catcactatc
     3781 aaatgagtac gcatccttca tgatgaactc cttgctgcgc ataacaccaa aacgaggtcg
     3841 tatctcatca cgaaattttg tattgatctg gtatgcattg acaggcaaat ccttatacga
     3901 ggcaactacc atacgaataa cattggtaaa tgcttcttca tgggtgggtg ccagtgcata
     3961 ctcaatgcca ttacggtctt ttatgcgtat catctccgga cccatggaat gccagcgccc
     4021 tgattccttc cataaatcgg cactggtaag ctcgggcatt aaaaactcta aagcacctga
     4081 tttgttcatc tcttccctga taatggtgat gatcttctgt aatacgcgca aacccaatgg
     4141 caaatagata tacatgccag ccgattcttt tcgtataagc ccggcacgca acatgagcct
     4201 gtggcttgta ataacagcat cagaaggatc ctccttcagt gtaggaacaa ggtagcgtga
     4261 cattttcata tagttttcct ctcatcaaat ggatattgca tgatgcataa cga
//
LOCUS       DAOSOX010000199         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21028_length_4226_cov_5.304004, whole genome shotgun sequence.
ACCESSION   DAOSOX010000199 DAOSOX010000000
VERSION     DAOSOX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21028_length_4226_cov_5.304004"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLX22_10980"
     CDS             complement(<1..644)
                     /locus_tag="PLX22_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20460.1"
                     /translation="MQHGKLYSIMLCVLLVTGCSDKVTVTECGIFAPSRLHYVKGQDG
                     VTPVDFDAQHTMWTFGDTITHEGMIANSLAFTNKVDASNIRELPFHYYTENGEIVPFI
                     RNDKHEDPSRDRLWAFDGIRINNTVYVYYVHVYIHDPLKPLSFSLMESSVAHWDVPYN
                     WSLGKPVRFKRFKNLFTGDVPALGASVLQHNDYVYVVGHISKNGKSSLVIARVKP"
     gene            796..>4226
                     /locus_tag="PLX22_10985"
     CDS             796..>4226
                     /locus_tag="PLX22_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOT20461.1"
                     /translation="MELKYGVTVWGNYFLEAIYQYYADIKRLQRGLRYANSDRVKKIE
                     INNTLVLAKVKGNQGRSYTVSLYFSPFKSDEKEKVVTLIEDNLLVLGEIIKGNLPDIL
                     LDELTKNNIKLFPSDWKELNRGCSCPDWGDPCKHMAAVYYLLTREIDLNPFLLFTLRG
                     LDLTKLFKITTKSLIDYPINIQEAHENQYNNELQSFREVAIVTINNYTNFILSKLSNN
                     PSFIDKDFKPIIEKFYKKSQALSDAILLRDIASMDSDLLLKLENLFKSGKISFIPDKN
                     FVGSYFTFYSELIEHEPVRQKIDSLNIMVYDAKTSTYHISPLNMLKLFLQFTTMYESN
                     ESTSECLMYNFLFILSRVAYNFVKSNAFIPAVYLEKNQFYCIFRLLSSPVDIKKQIHN
                     VISAMPVSLTLPSLTGKNTKKSRGTMDKESTIKFMLACFFTEYASIIGTSMKSSLKLT
                     KQFHSTMYDAIFLSKPYSMERFNDKALPLAIANYFSIFELIESNLDFSVIIDKHNDEY
                     FLQFLVNNTTINQITEQKTKNIVLKVLSNFIDVLPEIPLLLKNSTLPVSQERLEDLIT
                     KKLELLQNLGVEVLLPKELKEIIKPQLKIKTRLKDKTHVSFFSLNDMVTFDYEFYIDN
                     ETISYEELIALMNQNKELLKFRDSYIRINTTDIAKLMQQYETKSNLSQFDLLKSHLSN
                     ESETIELCDEIKAMFETMFTEQQYQLPHLNASLREYQERGFNWMLHLLLHGFGVILAD
                     DMGLGKTLQAIAVIQFLKENGYIKKPMLIVVPKTLMFNWESEIKKFSENLSYTFYYGT
                     KRTFSDTDIIFTTYETLQRDYKKLEDIVYDGIILDEAQKIKNYQTLTAKAIKHLSKKA
                     KFRIALSGTPIENRLTELWSIFDFILPGYLKSIDYFVNEYTKEIELKRNQQKIENLRK
                     ITSPFILRRLKTDKSIIKDLPEKIIIDQFVEMEKEQIALYEHVVQQLREEIAASENLL
                     PIILKSFTILKQICNHPRNYDKKSPAISHISGKTKLLLEILPAIIAKQEKVLIFTQFV
                     EMGNILKQIIENELLSEPLFLTGNLSQIKRKELIEKFEHDDSAQIFILSLKAGGVGLN
                     LTKANHVIHYDLWYNPSVENQATDRAFRIGQKKNVFVHRFVTKSSFEEKINAMIQ"
BASE COUNT         1525 a          738 c          674 g         1289 t
ORIGIN      
        1 ggtttaaccc gtgcaataac aagtgaactt ttaccgtttt tgcttatgtg cccaaccaca
       61 tacacataat cattatgctg cagcacagaa gcccccaaag caggtacatc ccctgtaaaa
      121 agatttttga accttttaaa gcgtacaggt tttcccaatg accagttata gggcacatcc
      181 cagtgagcaa ccgagctttc catcaatgaa aatgacagag gtttcaacgg atcatggata
      241 tagacatgga catagtatac atacaccgtg ttattaatcc gtataccatc aaaagcccac
      301 aatctgtctc ttgaagggtc ctcatgtttg tcattccgga taaatggaac aatttcccca
      361 ttttcagtgt aatagtggaa tggtaattcc ctgatattgc ttgcatcaac tttattggta
      421 aatgccagac tattagctat catcccctca tgagtaatgg tgtcaccgaa tgtccacatg
      481 gtatgctgtg cgtcaaaatc tactggcgtt acaccatcct gccctttaac atagtgaaga
      541 cgtgaaggag caaagatacc gcattcagtt actgtaacct tatctgaaca accagtaacg
      601 agtaaaacac ataacataat gctataaagt ttaccatgtt gcatactgta tgattagcct
      661 ttaccttttt tactatcgta tgactatcga taaatactac tttttttcaa ttattttttc
      721 tttttttgtt gattaaacgg ctttatcaga gctattagta atttttatta taatcttttt
      781 ttatagagca tggctatgga attgaaatat ggcgtaacgg tatggggtaa ttatttttta
      841 gaggctatct accagtacta tgctgacata aaacggttac agcggggatt gcgatatgca
      901 aatagcgaca gagtaaagaa gattgagatt aacaataccc ttgttttagc aaaagtaaaa
      961 ggaaatcaag gcagatcata tacagtgtcg ctctatttta gccctttcaa aagcgacgag
     1021 aaagaaaagg ttgttacgct catagaagac aatctacttg tattgggcga gatcatcaaa
     1081 ggcaatctac ccgatatact gcttgatgaa ctaacaaaaa ataatattaa attattccct
     1141 tctgactgga aggaattgaa tagaggatgc agctgccctg actggggcga cccgtgcaag
     1201 cacatggcag cagtatacta tctattaacc agagaaatag atttaaatcc atttttatta
     1261 tttacattac gtggattgga tttaacaaaa ctgttcaaaa tcaccactaa atcgctcatt
     1321 gattatccca tcaatattca agaagcacac gaaaaccaat acaacaacga attgcaaagc
     1381 tttcgcgaag tggcgatagt tactattaat aattacacta attttatatt atcaaaacta
     1441 tcaaataatc catcatttat tgataaagat tttaaaccca ttattgaaaa attttataaa
     1501 aaatcacaag ccttaagcga tgctattctt ttgagggata tagcatcgat ggacagtgat
     1561 ttattgttga agctcgaaaa tctttttaaa tccgggaaga tctcttttat accggataaa
     1621 aattttgttg gttcatattt taccttctat agtgagctca ttgaacatga accggtgcgt
     1681 caaaaaatag atagcctcaa cataatggta tatgatgcca aaaccagcac ataccatata
     1741 tcaccattaa acatgctcaa attatttctt caattcacaa ccatgtatga atcaaacgaa
     1801 tctactagcg aatgtttgat gtataatttt ttgtttatcc tttcgcgtgt tgcatacaat
     1861 tttgtaaaat caaatgcctt tatccctgct gtttatctag aaaaaaatca gttctattgt
     1921 atctttcgat tgctttcatc gcctgttgat attaaaaagc agatacataa tgtaatatca
     1981 gccatgcctg tctcactaac attgccatca ttgaccggta aaaatacaaa gaaaagccgt
     2041 ggtaccatgg ataaagaatc cactatcaaa tttatgctgg catgtttctt tactgaatac
     2101 gcttcaataa ttggaacatc aatgaaatct tcgttaaagt taacaaagca atttcacagt
     2161 accatgtacg atgctatatt tttatcaaaa ccatatagta tggaacgctt caatgataaa
     2221 gctttaccat tggctattgc taattatttt tctatctttg aacttataga atcaaatctt
     2281 gatttttctg ttatcataga taaacataat gatgaatact ttttgcaatt tttagtcaac
     2341 aatacaacca tcaatcaaat taccgaacaa aagacaaaaa atatagtact gaaagtttta
     2401 tcaaacttca ttgatgtttt acctgaaata ccgttactat taaaaaatag cactctgcct
     2461 gtatcacagg agcgattgga agatctcata acaaaaaaac ttgaactgtt acaaaacctt
     2521 ggggtagagg ttttactacc aaaagaatta aaagagatta taaaacctca acttaaaata
     2581 aaaacgcgct taaaagacaa aacacatgta tcgtttttta gccttaacga tatggttaca
     2641 tttgattacg agttttatat agacaatgaa acaatttcct atgaggaact tattgcttta
     2701 atgaaccaaa ataaggagct acttaaattt agagatagct atatacggat taacacaact
     2761 gatattgcca agctcatgca acagtatgaa accaaatcaa acctgtcgca atttgatctt
     2821 ttaaaatctc atctcagcaa tgaatcagaa acgattgaac tgtgtgatga aatcaaagca
     2881 atgtttgaaa ccatgttcac agagcagcag taccagcttc ctcatctcaa cgcatcgctg
     2941 agagaatatc aagaacgagg ctttaactgg atgctccatc tgcttttaca tggatttgga
     3001 gtaattttag ccgacgacat gggtttggga aaaacattac aggcaatagc tgttatacaa
     3061 tttttaaaag aaaatgggta cataaaaaaa ccaatgctta ttgttgttcc caaaacatta
     3121 atgtttaact gggaatcaga aattaaaaaa ttttctgaaa atctttcgta tacattttat
     3181 tatggaacta aaagaacatt cagtgatact gatatcatat ttaccaccta cgaaacatta
     3241 cagagggatt ataaaaaact ggaggatatt gtatatgatg gcataatcct tgatgaggca
     3301 cagaagataa aaaattatca aacattaact gcaaaagcta ttaagcatct atcaaagaaa
     3361 gcaaagtttc gtattgcatt gagtggcaca cctatagaaa ataggcttac agagctctgg
     3421 agtatctttg attttatatt gccagggtat ttaaaatcaa tagattattt tgttaacgaa
     3481 tacacaaagg aaatagaatt aaagcgtaat cagcaaaaaa ttgaaaattt acgaaagatt
     3541 acatcaccgt ttattttgcg caggcttaaa actgacaaat caatcatcaa agatttaccc
     3601 gaaaagataa tcattgatca atttgttgaa atggaaaaag agcaaattgc actctatgaa
     3661 catgttgttc agcaactcag agaagagatt gccgcaagcg aaaatctgct tcccattata
     3721 ttgaaaagct ttacaatatt aaagcaaata tgcaatcatc cccgaaatta tgataaaaaa
     3781 agtcctgcta tttcacatat ctcagggaaa acaaaattat tgcttgagat tttaccagca
     3841 attatagcga aacaagaaaa ggtattaatc ttcacccaat ttgtagaaat gggaaatata
     3901 ttaaaacaaa ttattgaaaa tgaattatta tcggaaccat tatttttaac cggcaattta
     3961 tctcagatta aaagaaaaga actcattgaa aaatttgaac atgatgacag cgcacaaatc
     4021 tttatacttt cattaaaagc aggaggggtt ggtttaaatt taacaaaagc aaatcatgtt
     4081 atccattatg atctttggta taacccatct gttgaaaacc aggctaccga tagagccttt
     4141 agaattgggc agaaaaaaaa tgtctttgta cacaggtttg ttactaaaag ttcatttgaa
     4201 gaaaaaatca atgccatgat tcaaca
//
LOCUS       DAOSOX010000200         4209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21142_length_4209_cov_7.706548, whole genome shotgun sequence.
ACCESSION   DAOSOX010000200 DAOSOX010000000
VERSION     DAOSOX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21142_length_4209_cov_7.706548"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLX22_10990"
     CDS             complement(<1..407)
                     /locus_tag="PLX22_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT20462.1"
                     /translation="MRCKNCHVVGIVFVLMFVVAIASGEVAPETALYDNHGKRYVLSQ
                     LLQKVQPQGIAILYFTSIHCKPCEKEIPELLQCIKKSQKNVLCYFVYAEGIEEVNAQA
                     SQYGITCYFTDVLGTTRTSMDVVRFPTIILLRQ"
     gene            672..860
                     /gene="rpmB"
                     /locus_tag="PLX22_10995"
     CDS             672..860
                     /gene="rpmB"
                     /locus_tag="PLX22_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT20463.1"
                     /translation="MAKCEVCGKSILFGHNVSHSNKKTGKIWHANVQKIRVVEGNKVV
                     RKNVCTRCIRSGAVRKAI"
     gene            complement(861..1442)
                     /locus_tag="PLX22_11000"
     CDS             complement(861..1442)
                     /locus_tag="PLX22_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20464.1"
                     /translation="MIQILDYWYSALIDPEKLSQIIAVEDKKHFIVSAVVVAIVVFMD
                     IVAQSLLSMQTGFFYYKITYGFIALFLLDVLCLMIYAGLLTVILQIGGYQASGGYIIN
                     ALALSQIPRIFIVPVVLIFKSLYFAPVFFYSAASLALFIWSLFIAMRCISQRYQIESL
                     KTIGYIALPAILAGVTAFLILVAGGMVLIGLIK"
     gene            complement(1445..2374)
                     /gene="sppA"
                     /locus_tag="PLX22_11005"
     CDS             complement(1445..2374)
                     /gene="sppA"
                     /locus_tag="PLX22_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOT20465.1"
                     /translation="MDKNRRILIAILAMVVFAVVLAIIQISITMQRQMTIGTRINLTE
                     GPGVGIVRVYGRIEATGDMMAQANADYIVKRLDALAQDSRIRAIVIRINSPGGTVAAT
                     QEIYTKILSLRKKNIPIIASMGDVAASGGYYIASACNAIVVNYGTITGSIGVIAMAPN
                     LKGLFDKLGIRMNVIKSGRYKDILAMHREISQEELQLIQQIIDSSYQKFVHDVAAGRN
                     MAVSDIMPYADGRVFTGNQAVEYKLADELGSYDDALNKARTLAKLPDDSAVYDDTASP
                     IERLLMTMSSFFKPKLEQMVNSQYSLVEYSYLP"
     gene            2775..3821
                     /locus_tag="PLX22_11010"
     CDS             2775..3821
                     /locus_tag="PLX22_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20466.1"
                     /translation="MKKCFIIVAIVLAILCAVAFAIFFHYWQQPSVKYLLTMGGYLPV
                     YGISITEERITVNNKTVPLDIYRKKNAHSNTYFVLFHGLTPQAHKHPQLVQMAKSIAF
                     ATGMNVIVPKIPEYFIKDATLHDVKQGIRSLYSSIVNAYPGHYRAFGACLSGTVMLSA
                     FTMLPQNIYPEKILLFGPMFQGDTIKGTLFEKYFDVDFLVKLTISSNMSMFSPEEKDL
                     IHKAMMSANMGITDETRMKQILGDRLFRDISIVTIDEEYLKKTGARTLLKNYKAIPNC
                     KYFILHSKTDRIIPFDEGKSLFNFLKGSGTFSEFLGTELLDHVSGRITVKGFVSELKY
                     MKHFFDELFEGDSY"
     gene            complement(3866..>4209)
                     /locus_tag="PLX22_11015"
     CDS             complement(3866..>4209)
                     /locus_tag="PLX22_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20467.1"
                     /translation="AEMYNKITGRTGPFWNERYKDIIVQFAHDAFHYLLWLLWYIAFN
                     PVRKRRCDSPIKYRYSSIQSYLQEDADVGVPIDHHEYFLQLGNSFSERVRRFLQYEEA
                     YRKRYSIAQWV"
BASE COUNT         1283 a          934 c          776 g         1216 t
ORIGIN      
        1 ttttgccgta acagtataat agttgggaaa cgtacaacat ccatgctggt acgtgtcgta
       61 cccagcacat ctgtaaaata gcacgttatg ccatactgtg aagcctgcgc atttacctct
      121 tctatgcctt cggcatagac aaaatagcac agtacatttt tttgtgattt tttaatacat
      181 tgcaatagtt ccggtatctc tttttcacac ggtttacagt gtattgacgt aaaatatagt
      241 attgcaatcc cctgcggttg tactttctgc aataactggg aaagcacata acgcttgcca
      301 tgattatcgt acagcgctgt ttcaggtgcg acctcgccgc tggctatggc taccacaaac
      361 attaatacaa aaacaatacc tacaacatga cagtttttac aacgcatacg ccatcccctt
      421 tttttgtttg gtattgtata ttgtagcaat tacatccatg gcaagttatt tcaagtaaaa
      481 aattacgtgt ctgccacata aaattttgtg acagtgaaat aaaatccttg caatttttat
      541 atgccattac cactattgtc catcgttggt agcataatat taatttccag taactatcgc
      601 ccataaaaat attttttttg cttttaaaaa tgctattata tagtaaataa ttaccagtaa
      661 gggagatacc aatggctaaa tgtgaagttt gtggcaaatc tatactgttt ggtcataatg
      721 taagccattc caataaaaaa accggcaaaa tatggcatgc aaatgttcaa aaaattcgcg
      781 tggtagaggg aaacaaagta gtccgtaaaa acgtatgcac acgctgtatc cgctcgggag
      841 cagtgcgcaa ggctatataa tcactttatt aaaccaatta aaaccatacc accagccacc
      901 agtatcaaaa aagctgttac ccctgccagt attgcaggga gggctatata tcctattgtc
      961 tttaaacttt ctatctgata gcgctgggaa atgcagcgca ttgcaataaa caatgaccat
     1021 ataaacagtg ctaacgatgc agcgctgtaa aaaaataccg gtgcaaaata taatgatttg
     1081 aatatcagta caacaggcac aataaaaatc cggggaatct gcgaaagcgc cagcgcattg
     1141 atgatatagc cacccgaagc ctgatagccg cctatctgca atattaccgt tagcaaccct
     1201 gcgtatatca taaggcacaa cacatcaagt aaaaacaatg ctataaagcc ataggtaatt
     1261 ttatagtaaa aaaagcctgt ttgcattgaa agcagtgatt gcgccacaat atccataaag
     1321 acaactatcg ccacaaccac agcgctgacg atgaaatgct ttttatcctc aaccgctata
     1381 atctgcgaca gcttttcagg atcaatcagc gcactatacc aataatccaa tatctgtatc
     1441 acattcatgg caggtaacta tattccacca aagaatactg tgagtttacc atctgctcta
     1501 actttggttt aaaaaacgat gacatcgtca tcagtaaccg ttcaatgggg cttgccgtat
     1561 catcataaac ggctgaatcg tccggcagct tagctaatgt acgcgcctta tttaatgcat
     1621 cgtcatagct gcccagctca tcagcaagtt tatactcaac cgcctgattg ccggtaaaca
     1681 cacgcccatc agcatacggc atgatatcgc ttaccgccat atttctgcct gctgctacat
     1741 cgtgcacaaa cttctgataa cttgaatcaa taatctgttg aatcaactgc aattcttcct
     1801 gcgaaatctc tctgtgcatg gcaaggatat ccttatacct tccgcttttt attacattca
     1861 tacggatacc caacttatca aataagcctt tgagattggg agccatggca ataacgccaa
     1921 ttgagcctgt tatggtgcca tagttgacaa caatagcatt gcacgccgaa gcaatgtagt
     1981 aaccgcctga tgctgccaca tcacccatcg aagctatgat gggaatattt ttttttctaa
     2041 gcgatagtat cttggtatat atctcctgtg ttgcagcaac cgtcccgcca ggagaattaa
     2101 tccgtataac tatcgccctg atgcggctgt cctgtgctaa tgcatcaagc ctttttacaa
     2161 tgtaatcggc attagcctgc gccatcatat caccggttgc ttcgatacgt ccatacacac
     2221 gcacgatgcc aactccggga ccctcggtga ggttaatccg tgtaccaatg gtcatctggc
     2281 gctgcatggt tatgcttatc tggattattg caagtacaac cgcaaaaacc accattgcca
     2341 gtatggctat aagtatccgt ctgtttttat ccatacctta tcccctgcca atatttgaca
     2401 acaatacacc atgtacagca ataaatgcaa catttttttc ttgctttttt ttggcgatag
     2461 ttactattaa aaccgataag tcggtatagt ataaaaccgt tgctctttat ggaaaatcag
     2521 tacgcattgc cgggtgaata atgttctggc agcgttatag caatccagcc gtgctcaggc
     2581 gaaatcaaag cgaagaatct cgccgttgta tgtgcatgtc atcctgaact ctgattcggg
     2641 atctaatgta agcaaaaggg attccgggtc aagcccggaa tgacactggt gtaaaaactc
     2701 ccctcgcctt gcaaagaaga gtggcaggag gttaggttca taatcatagt ttgaaaagga
     2761 ggagtaataa cccaatgaaa aaatgcttca ttattgtagc catagtcctt gcaatactat
     2821 gtgccgttgc ttttgcaatt tttttccatt actggcagca gccatcggtt aaatatcttc
     2881 ttaccatggg aggatacctt cctgtttatg gcatatccat taccgaagaa aggataacag
     2941 taaataataa aactgtacct cttgacatct atcgcaaaaa aaatgcgcat agcaatacat
     3001 actttgtact gttccatggt ctgactcccc aggcgcataa acatccccag cttgtacaaa
     3061 tggcaaaatc cattgccttt gctaccggga tgaacgtcat tgtccctaaa attccggaat
     3121 attttattaa agatgcaacc cttcacgatg ttaaacaggg aatacgttct ctttacagca
     3181 gtattgtcaa tgcttatcca ggacattacc gtgcatttgg tgcatgctta agcggcactg
     3241 ttatgctcag tgcatttacc atgctgccgc aaaacatata tcctgaaaaa atattgttat
     3301 ttggtcccat gtttcagggg gataccatta aaggcacctt gtttgaaaaa tattttgatg
     3361 tagatttttt ggttaaatta acaatttcat ctaacatgtc tatgtttagc ccagaagaaa
     3421 aagatctcat ccacaaagcc atgatgagcg ctaatatggg cattaccgat gaaacaagga
     3481 tgaaacagat actgggtgat aggctatttc gcgatatctc tatagtaaca atcgatgaag
     3541 agtatctaaa aaaaactgga gcacgtacac ttttaaaaaa ttataaagcc atccccaatt
     3601 gcaaatattt tattcttcat agtaaaaccg acaggatcat cccttttgat gaagggaaat
     3661 cgctgttcaa ctttttaaaa ggaagcggta cattttctga atttttaggc acagaacttt
     3721 tagatcatgt atccggacgc attaccgtca aaggttttgt cagtgaactg aaatatatga
     3781 aacacttttt tgacgaactt tttgagggcg acagttactg agtactgggg ttttccttat
     3841 aatgctataa ggctctgacc cctttttata cccactgtgc aatggagtag cgcttgcgat
     3901 acgcctcctc atactgtaaa aaacgtctta cccgttcact gaaactgttt cccaactgca
     3961 gaaagtactc atgatggtct atcggtaccc caacatcagc atcctcctgt aaataggatt
     4021 gtatactgct gtagcgatac ttgataggac tatcgcacct gcgcttgcgt accggattaa
     4081 acgctatgta ccacagcaac cacaagagat aatgaaaggc atcatgtgca aactgtacaa
     4141 tgatgtcctt ataccgctca ttccagaatg gcccggtcct gccggttatt ttattataca
     4201 tctcagcaa
//
LOCUS       DAOSOX010000201         4193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21259_length_4193_cov_7.689705, whole genome shotgun sequence.
ACCESSION   DAOSOX010000201 DAOSOX010000000
VERSION     DAOSOX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21259_length_4193_cov_7.689705"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..911
                     /locus_tag="PLX22_11020"
     CDS             93..911
                     /locus_tag="PLX22_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOT20468.1"
                     /translation="MEYKYYLVEKKPPVAWVFLNRPEKKNAMNPAAWWESPSVFKELD
                     ADDDIRVIVLAAKGASFTVGIDLIEMAGEIKELAEPSQKGGIKRSLLQKIYRMQDTMT
                     CIEKCRKPVIAAVHGYCIGAGLDMIASCDIRLASADAQFSLREAAVGFVADVGVLQRL
                     PHIVGQGITRELAYTAKYITAQRAKEISLINEVFADQKALYAGAEKMAMEIAENSPLA
                     VQASKDVLNFGIGKTTDEALKYVASISANIIPSDDLFEAFTAFAQKRKPNFTGK"
     gene            complement(975..>1805)
                     /locus_tag="PLX22_11025"
     CDS             complement(975..>1805)
                     /locus_tag="PLX22_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20469.1"
                     /translation="NALVHNIEIDPKDLKNKNQVKLTVDIGIKTIGKTSTGSVESFDI
                     GLSAKGNVKPFDLKTRILNPEIALKAGSPYGTMTGLQIFDAIKSNETLANYAGKFDFL
                     AKDVKWKNGYIDIWYKGGLLKITNGKISTDDYAGNFKADINLNTSTVNADIDMILNEK
                     HNKSIYNGIKGNASKLIKGDVAKYVKPENVADQAFKYLTNKDGKVYLKFAVTGPMNKP
                     KTKLVEPKLPSLNDIIKDMAGDVVDVAKEKAVKKVSQTVEKTTDKAKDKATKKLKKLF
                     "
     assembly_gap    1806..1905
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1906..2560)
                     /locus_tag="PLX22_11030"
     CDS             complement(<1906..2560)
                     /locus_tag="PLX22_11030"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOT20470.1"
                     /translation="MKRFLKWLAIVIGGLIALVIIASIVLMLIINKDMIAQQAEKVLN
                     RHVTIESIDVSIFSVLSGIEVKGVAISNFKTPQQLSALKGKPVDKADLFAGLDSFTFK
                     LKILPLLQGKFELRELLLSGPKVNIVRYKSGAFNFSDLMKPSTKEEKKVEEVKKEEPS
                     KPLTADTLPVSITIGRIGIEKGTVTFTDQSSGQKVMLYNCNALVHNIEIDPKDLKNKN
                     "
     gene            2930..>4193
                     /locus_tag="PLX22_11035"
     CDS             2930..>4193
                     /locus_tag="PLX22_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOT20471.1"
                     /translation="MKSKIIITVSFVLCVGYALFLGCKSTPHYRLAQFISPSTCGGCH
                     DEIYEQWKNSMHNLSQKDVIYKEFARFYLDDSKSQNPVNNDELKEAESCVKCHIPVGY
                     ITGKPVSVTEELSMEKDLPEITMEGIQCDYCHTIVEAKKYYNARFVYKPGNGEADPGI
                     KRGPFKDANPDWHTAEYSSFHTKASFCGTCHDVRHVVYGTKLETTFEEWTKSPYNTGD
                     SKTTVPCQGCHMYHRPGVPATGSTPRPDNPGFAAFGGPQRDHIFTHYFVGGNSVIPSL
                     YKDTVKAQMAQERLKNAAVVEIETSDSNSGTVGIAVTNIGAGHKLPTGLTDVRQMWLE
                     IVVVNQYGREVYAVGKADKKGYLPDNTIIFKTIFGDGSGKPVMNIAKAREILFDNRIP
                     PKQKVTSTIVLPKGLKGNYTIKVRLLYRI"
BASE COUNT         1231 a          707 c          850 g         1305 t
ORIGIN      
        1 aacacaataa aacataacag tgtaattttt aattgataaa taaatgcatg gtgattacta
       61 tgttcataaa tattgtaata ggggtataga ctatggaata caaatattat ttagtagaga
      121 aaaagccgcc ggttgcatgg gtctttttga acaggcctga aaagaaaaat gctatgaatc
      181 ctgcagcgtg gtgggagtcg ccgtctgtgt ttaaagagct ggatgctgat gatgatatac
      241 gagttattgt cttagctgct aaaggggcat cctttacggt tgggattgat ttgattgaga
      301 tggctggtga aataaaagag cttgccgaac catcccagaa aggagggatt aagcgaagcc
      361 tgttacagaa aatttataga atgcaggaca ccatgacatg cattgaaaaa tgccgtaaac
      421 cggttattgc cgcagtgcat ggatactgca ttggtgccgg gctagatatg atagcctcgt
      481 gtgatatcag gctggcaagt gctgatgcac agtttagcct tcgtgaagct gcggtaggat
      541 ttgtggctga tgttggtgtt ttgcagcgcc tccctcacat tgttggtcag ggcattacac
      601 gtgagttagc ctataccgca aaatatatta ccgcacagcg tgcaaaagag atatcgttaa
      661 ttaatgaagt gtttgctgat caaaaggcac tgtatgcagg ggctgagaag atggctatgg
      721 agattgctga aaattcacca ttagcggtac aggcatcaaa ggatgtatta aactttggta
      781 ttggtaaaac tactgatgag gcattaaaat atgtagcatc aataagtgcc aacatcattc
      841 cttctgatga tctatttgaa gcctttacgg catttgctca gaagcgaaag ccaaacttta
      901 ccgggaaata ataatgaacc ctcattatcg ccctctcctt aaaaaaagga gagggagtaa
      961 tgatgaggtt tgatctaaaa caacttttta agtttctttg tagctttatc ttttgctttg
     1021 tcggttgttt tttctactgt ttgtgatact tttttaacgg ctttttcttt agcaacatca
     1081 acaacatcgc ctgccatatc tttaataata tcatttaaag aaggaagctt tggttctaca
     1141 agttttgttt taggtttatt cattgggccg gttacagcaa atttaaggta aaccttcccg
     1201 tctttattgg ttaagtattt gaaggcttga tcagcaacat tttcaggttt tacatatttt
     1261 gcaacatcac ccttgataag tttagaagcg ttgcctttta taccgttata gatactctta
     1321 ttgtgcttct cgttgaggat catatcaatg tcggcattaa ctgtgctggt gttaagattt
     1381 atgtcggctt tgaagttgcc tgcataatca tcagtgctta ttttaccgtt agtgatcttc
     1441 agcaatcctc ccttgtacca tatatcaata tagccgtttt tccatttaac atcctttgcc
     1501 aggaaatcaa atttcccggc gtagtttgcc agtgtttcat tgctctttat agcatcaaat
     1561 atctgtaatc ctgtcatggt accatagggg cttcccgctt taagcgctat ttcaggattt
     1621 aagatacgtg ttttaagatc aaatggtttt acgttgcctt ttgcagatag ccctatatca
     1681 aatgactcga cactcccggt acttgtcttg ccaatggtct tgatgcctat gtctacggta
     1741 agctttacct gatttttatt ctttaaatct ttaggatcaa tttctatatt gtgcaccaat
     1801 gcattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngattt ttattcttta
     1921 aatctttagg atcaatttct atattgtgca ccaatgcatt gcaattatag agcattacct
     1981 tttgccctga tgattgatcg gtaaaggtga cagttccttt ttctatgcca atcctgccaa
     2041 tggtaatact taccggcaat gtatctgctg ttaaaggttt tgatggttct tcttttttaa
     2101 cctcttctac ttttttctct tcttttgtag atggtttcat taaatcggag aaattaaatg
     2161 caccgctttt atagcgtaca atatttacct tcggaccact gagtaaaagt tctcttagct
     2221 caaatttacc ctgtaacaat ggaaggattt tgagcttaaa ggtaaacgaa tcaagccctg
     2281 caaaaagatc agctttatca acaggttttc cctttaatgc tgatagttgt tgcggtgttt
     2341 tgaaattgga tatagcaacg cctttcacct caatgccaga aagcacactg aatatactga
     2401 catcaatgga ttcaatggtt acatgacggt taaggacttt ttctgcctgc tgagcaatca
     2461 tatctttgtt gatgatgagc attaaaacaa tactggcaat aataaccagt gcaatcaatc
     2521 caccaataac tatcgcaagc cattttaaaa atcttttcat aactccctcc agtaattata
     2581 taattgttgt aataaatgta ttaataagta tagtaaaaca tattttggtt acattcagag
     2641 taattaacta tgtattgtaa tatgatagct aaccattcta tattagcaag caaaaatttt
     2701 cttgaaaaca aatgggatat tttcaacaat aacgtaattg acatttttgt ttataaatac
     2761 tgaaatgaac aagaaatagt gatgctatag catagtaatt gaaatgaata gtttatcttt
     2821 gtgagtaagg tagtaaaagc agtcattgta taattttaaa tatgatagtt gaagatagta
     2881 attactgtat agtgcaatga tgagaataaa caggtgtaag ggatacagta tgaaatcaaa
     2941 aataataatt accgttagtt ttgttttatg tgttgggtat gcactttttc ttggctgcaa
     3001 atcaacgcct cattatagat tggcacagtt tataagtcct tccacatgtg gtggttgtca
     3061 tgatgagata tatgaacagt ggaaaaattc aatgcataat ttatctcaaa aggatgtcat
     3121 ctataaagag tttgcacgat tttatctgga tgatagcaaa agtcaaaatc ctgtcaataa
     3181 tgatgaacta aaagaggcag aaagctgtgt aaagtgccac atccctgtgg gatatattac
     3241 tggtaagcca gtatcagtaa ctgaagaatt atccatggag aaggacctgc cggaaataac
     3301 catggaaggt atccagtgcg attactgtca tactattgtt gaagcaaaga aatactataa
     3361 cgcacgattt gtgtataaac cagggaatgg cgaggcggat cccgggataa aacgtggtcc
     3421 ttttaaggat gctaatcctg attggcatac agcagaatat tcttcgttcc atacaaaagc
     3481 tagtttttgt gggacatgcc atgatgtacg ccatgtggtg tatggaacaa aattggagac
     3541 tacctttgag gaatggacaa aaagccctta taacaccggt gactccaaaa caacggtgcc
     3601 ctgtcagggt tgtcacatgt atcaccgacc aggggtgcct gcaactggct ccacacctcg
     3661 tcctgacaat cccggttttg cagcgtttgg tggtccacag cgtgatcata tctttactca
     3721 ttattttgtt ggcggaaata gtgtcatccc ttcattatac aaggataccg tgaaagccca
     3781 gatggcacag gaacggctga aaaacgcagc agtagtggaa attgaaacca gtgacagtaa
     3841 tagtggtacg gttggtattg ctgtaaccaa tattggtgcc ggtcataagc ttccaacagg
     3901 gcttactgat gtgcggcaga tgtggcttga gatagtagtt gtcaatcagt atggaagaga
     3961 ggtgtatgca gtagggaagg ctgataaaaa gggttatttg cctgataata caatcatttt
     4021 taaaacaatt tttggtgatg gtagcggtaa accagtgatg aatattgcaa aagccaggga
     4081 aatactcttt gataacagga ttccacccaa acaaaaagtt accagcacca ttgtgttgcc
     4141 taaagggtta aagggtaact atactatcaa ggtgcggttg ctgtacagaa ttg
//
LOCUS       DAOSOX010000202         4164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21437_length_4164_cov_8.244098, whole genome shotgun sequence.
ACCESSION   DAOSOX010000202 DAOSOX010000000
VERSION     DAOSOX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21437_length_4164_cov_8.244098"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLX22_11040"
     CDS             <1..195
                     /locus_tag="PLX22_11040"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT20472.1"
                     /translation="ITMLSEITSVQVVIEHDQSKMRPAEVQTIIGSYQKINTLTGWSP
                     TINIRQSLEDIVKGQSSALH"
     gene            complement(192..377)
                     /locus_tag="PLX22_11045"
     CDS             complement(192..377)
                     /locus_tag="PLX22_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20473.1"
                     /translation="MKRLVFFFIIATVIFASHSGFAANTISEVPIRIFFELVPALRIA
                     PDVDFGIRGGLGARYFF"
     gene            782..1231
                     /locus_tag="PLX22_11050"
     CDS             782..1231
                     /locus_tag="PLX22_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HOT20474.1"
                     /translation="MRLRQTLLKILINFYPPFLGAGIRIRFDKKNKKVYVSMKLTWYN
                     RNYVGTHFGGSIYAMCDPFYMFLLMEILGSNYIVWDKKATIDFIKPGKSTLHAEFFID
                     EQTVEAIKTEVEQKGKALPQFTVAVKDDDGEIIARVDKYIYVKKKKT"
     gene            1228..2184
                     /locus_tag="PLX22_11055"
     CDS             1228..2184
                     /locus_tag="PLX22_11055"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT20475.1"
                     /translation="MIYPATDITIQQAAEAIKQGEVVAFPTETVYGLGANAFDRLAVA
                     SIFAIKERPHFDPLIVHVNSLSMVSMVAAAIPCAAMKCMEKFWPGPLTIVLPKKDDIP
                     DIVTAGLATVAIRMPLHPVALRLIELAGVPVAAPSANPFGYLSPTTAQHVAMQLGNKV
                     AIILDGGKCAVGVESTIIKIGNDEINVLRYGGIPVEEIERTTGLVVTSHTTTIEAPGQ
                     LPYHYAPQTKIVLVSDIMSVPDDATSGLLLFKKRDIPYTSIHIMYLSDEGDMRQAAGN
                     LFSCLHALDAMKLKVIYAEQVEEVGLGRAIMDRLKKAQARFM"
     gene            2488..3879
                     /locus_tag="PLX22_11060"
     CDS             2488..3879
                     /locus_tag="PLX22_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT20476.1"
                     /translation="MIRFDYNYFMDEHIGTQHGLSQNDIDGIRPEFNEVLKRMDDEKA
                     EGLLGFLEIPFNTSLVKDIQSFRQHTDWCDTIALIGIGGSALGPQALKNALADIYWNE
                     LPDEKRGKRCKVYFFDNVDPIEMNSLLDIINIPTTLFLVISKSGGTTETNANFAILLD
                     TIKKNTRDYKKHIVTITDPAKGIMRQITREEGFTSFPIPANVGGRFSVLTAVGLVMAE
                     FIGIDINGLLHGAAEVIDRYYGKEVWSNAPLLNAAFHYCFDVKKNKRINVLMPYTRKL
                     FLFADWFRQLWAESLGKRFDIYGNEVMVGLTPVAALGTVDQHSQLQLYLEGPNDKVIT
                     FLKLQDFGTDITIPEFYKGKAELDYLSGKSLRKLNGFEEQATELVLKEASRPNASIIL
                     PELDERSAGQLIMFLEMQTAYAGYLYNINPYDQPAVEQGKRFTFGLLHRPGYDEYEQK
                     FKSGYQKKENYIL"
     gene            3876..>4164
                     /gene="tmk"
                     /locus_tag="PLX22_11065"
     CDS             3876..>4164
                     /gene="tmk"
                     /locus_tag="PLX22_11065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT20477.1"
                     /translation="MKIMTPLLIVFEGIDGAGKSTQATLLYAFCRECAKTALLQEPTD
                     SRWGKSIRKILQGELQATTQQMLDLFLLDRAYDVEHNIKPLLHKGYVVIVDR"
BASE COUNT         1326 a          732 c          883 g         1223 t
ORIGIN      
        1 atcaccatgt tatctgaaat aaccagtgtg caggtagtca tagagcatga tcaaagtaaa
       61 atgcgacctg cagaggtgca aaccattatt ggctcatatc aaaaaataaa tacattaaca
      121 ggatggagcc caactataaa catccgccaa tcactggaag atattgttaa gggtcagagc
      181 tctgccttac attaaaaaaa gtatcgtgcg cctaaaccac cacggatgcc aaaatcaaca
      241 tcaggtgcaa tcctgagcgc tggcactaac tcaaaaaata tccgtattgg tacttcacta
      301 atggtattag cagcaaaacc agagtgtgat gcaaaaatca cagttgctat tatgaaaaaa
      361 aatactaatc gcttcatagt agtctcccct tgctatagta aaccattgat aaaccgtttg
      421 tggcattgca ttgtttactg tgaatacata attttatcta taaaattata aaaaatactc
      481 tctactggca actgctaata cttacaatta ttatttatta tattatttat tactgtacac
      541 tgttcatgat gggcatcttc accaatcaaa tttttcattg atgaataata atccactatt
      601 gtgcactatt tgtgattatt gggggatagc gtaacaacaa ttattaaaaa actgctactt
      661 cacctgttat actgttactg agtaactatc gtgacaacaa aaagaaaatt ttttatctac
      721 ttttgtttgc ggtactatac tatatagggt aattattatt tcttaattaa taggtgctga
      781 tatgcgattg cgacaaacat tattgaagat tttaatcaat ttttatcctc catttttagg
      841 agcaggaata cgcattagat ttgataaaaa aaataaaaaa gtctatgtat ccatgaagtt
      901 gacctggtac aaccgtaatt atgtcggtac gcattttggc ggctccatct atgccatgtg
      961 cgatcccttt tacatgtttt tgctcatgga aattctggga agcaactata ttgtgtggga
     1021 taaaaaagca acaatagatt ttattaagcc cgggaaaagt acactgcatg ctgaattttt
     1081 tatagatgaa caaacagtgg aagctattaa aacagaggtg gaacaaaaag ggaaagcatt
     1141 gcctcaattc actgttgcag taaaagatga cgatggcgag attattgccc gggtggataa
     1201 gtatatttat gtgaagaaga aaaaaacatg atttaccctg caactgacat taccatacaa
     1261 caggcagccg aagcaataaa acagggtgag gtggtagcct tccccactga aacggtatat
     1321 ggattgggtg caaatgcatt tgataggctg gctgttgcaa gtatatttgc tataaaagaa
     1381 cgcccccatt ttgatccctt gattgtgcat gtcaattctc tgtccatggt ttccatggtg
     1441 gcggctgcca taccatgtgc agcaatgaag tgtatggaaa agttctggcc aggaccattg
     1501 acaatagttt taccaaagaa ggatgatatc cctgatatag taacagcggg gcttgcaacg
     1561 gttgccatac gcatgccatt gcatccggtt gcgctcaggc ttatagaatt agcaggtgtg
     1621 cctgtagcag cacccagtgc aaatcctttt ggctatttaa gcccaactac cgcacaacat
     1681 gttgctatgc agttgggcaa taaagttgct atcattcttg atggtgggaa atgtgctgtt
     1741 ggggttgaat ccaccattat aaaaattgga aatgatgaaa ttaatgtact tcgctatggc
     1801 ggtatacccg ttgaggaaat tgaaagaaca accggacttg tggtcacttc tcataccacc
     1861 acaattgaag ctccgggtca gttgccttac cattacgcac cgcaaacaaa gattgtactg
     1921 gttagcgata tcatgtctgt tcctgacgat gcaaccagtg ggctattgct ttttaaaaaa
     1981 agagatatcc cctataccag tattcacatc atgtacttat cggatgaagg tgatatgcgg
     2041 caggctgccg gcaatctctt ttcatgtttg cacgcgcttg atgctatgaa gcttaaggtt
     2101 atctatgctg agcaggtgga ggaggttggt ctgggcaggg caatcatgga tagattaaaa
     2161 aaagcacagg cgcgctttat gtagctatgg ataaaaagta aattgatatg gattatatga
     2221 aaagttacca ttataatgct taataacctg acaagaataa tagtatacaa aatgccgggg
     2281 cgatagcaat ggcttccttt gttgctcttt tcttactata agatagataa aagtaatagt
     2341 gcgctacttc cgaaacgtgc acacaaccag gattatagca acaatattat gattgacatt
     2401 ttgataatgt gcacatagta tcttgtgctt tgaaaataat taatagcaga atcttatccc
     2461 gggtattttg ataaaaagga gagcatgatg atacgctttg attataacta ttttatggat
     2521 gaacatattg gcacacagca tggattgtca caaaatgata tagatggaat aagacctgaa
     2581 tttaatgaag tccttaaacg catggatgat gaaaaggcag aaggattgct tggtttttta
     2641 gaaataccgt ttaacacttc cttagtcaaa gacatacaaa gttttagaca acatactgat
     2701 tggtgtgata ccattgcgct tattggtatt ggcggctcgg cgttgggtcc ccaggcactt
     2761 aagaatgcac tggctgatat ctattggaat gagttgcctg atgaaaaacg gggtaaacga
     2821 tgcaaagttt atttctttga taatgttgat ccaattgaga tgaactcact gttagatatt
     2881 atcaatatac caacaacgtt gtttcttgtt atatcaaaat caggaggaac taccgaaaca
     2941 aatgcaaatt ttgctatttt gttagataca attaaaaaga atacacgcga ttataaaaaa
     3001 catattgtta ccattactga tcctgcaaaa gggataatgc ggcagattac acgcgaggaa
     3061 ggttttacat catttccaat ccctgcaaac gttggcggac ggttttccgt attaacagct
     3121 gttgggcttg ttatggcaga atttattggt attgatataa atggtctctt gcatggagca
     3181 gcagaagtta tcgatagata ttatggcaag gaggtatgga gcaatgcacc gctgcttaat
     3241 gctgcatttc attattgctt tgatgttaaa aagaataaac gcatcaatgt gcttatgcca
     3301 tatacgcgaa aactatttct ctttgctgat tggtttagac agctttgggc agaatcgctg
     3361 ggtaaacgct ttgacatata tggcaatgag gttatggtgg ggcttactcc tgttgcagcg
     3421 ctgggtactg ttgatcagca ttctcagctt cagctttatc tggaaggacc aaatgataag
     3481 gtaattacat tccttaaatt gcaggatttt ggtaccgata tcaccatccc tgaattttat
     3541 aaagggaaag ctgagctgga ttatctttct ggaaaaagtt tgcgcaagct caatggtttt
     3601 gaggagcagg ctacagagct tgtattaaaa gaagcatccc gaccaaatgc ctcgataatt
     3661 ttaccggagt tagatgaaag atcagcagga cagcttatta tgtttttaga aatgcaaaca
     3721 gcctacgccg gatatctata taacattaat ccatatgatc agccagcggt tgaacagggt
     3781 aagcgtttta cctttgggct gctccaccgg cccgggtatg atgagtatga gcaaaagttt
     3841 aaaagcgggt atcaaaaaaa agaaaactat atactatgaa gataatgacg ccccttttga
     3901 ttgtctttga aggaattgat ggtgcaggaa aatcaacaca ggcgacatta ctgtatgcct
     3961 tttgcaggga atgtgccaaa acagccttat tacaggaacc aacggatagc aggtggggaa
     4021 aaagtatccg aaaaatattg cagggtgagc tacaggcaac aacgcagcag atgcttgatc
     4081 tttttctact tgatagagcg tatgatgtgg agcacaacat caagcctttg ttgcataaag
     4141 gatatgtggt gatagttgat agat
//
LOCUS       DAOSOX010000203         4151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21548_length_4151_cov_6.423584, whole genome shotgun sequence.
ACCESSION   DAOSOX010000203 DAOSOX010000000
VERSION     DAOSOX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21548_length_4151_cov_6.423584"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLX22_11070"
     CDS             <1..715
                     /locus_tag="PLX22_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT20478.1"
                     /translation="ITIIGNYLNACKPDVSILEIGTGMGIAAACLKKAGFDVHAIEPH
                     TAGFENRSLLYQRVYALLDIGDIVLPVTIEQMPLNKQYDFIFSFNVLEHVNRIEAAFR
                     VLSHILKPAGVMVHSCPNYYIPYEPHCNVLLLPFVPQYTHYICRRLRNSVGLSLTFTT
                     VGLIKKLAKTNNLAVTFKNDVLYNAFRRFEYDAAFAQRQKGLPLVVYKLLKKCRILWL
                     AKHVPVVMQTPMVFELKKK"
     gene            complement(717..2861)
                     /locus_tag="PLX22_11075"
     CDS             complement(717..2861)
                     /locus_tag="PLX22_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G-protein coupled receptor"
                     /protein_id="HOT20479.1"
                     /translation="MKIAKIVVTVILLLFIAAALFIGNTRPIDDGDTWWHMEYGQYML
                     EHKTLIPHHSLYTWTPASNDTVYCAWIPEIILYALYSAYGFYSLYAVKYALAVFALAL
                     FILYAIKRNVLTHPVSLFVILMGVITHGNSSGSMIKPELFTYVFMTIMVFLWYMFSLT
                     MQKNLAYLYGIPIVILIWVNSHGGFIFAAPFFVCTYIGILLNYKFQKDTTVNKKVLRH
                     ITIAWILSFLATFVTPYGYSYHLDLLQSILLKPDVENLKTVQAYTPTLAVPTFVLYFV
                     IPIIILLYTIISTKRYRLHTIDWVFIVNNVMYAALYTIFGRLIYYWIPVYVFSTLFYI
                     KGDAWYYTPKKRWIPAIITAIMLLAGLDQLRWHTFINFTANATWFGFGNSFINSDEEA
                     NFIAKYYPGYTIGNPYNCGGYFLWRMYPDNKIMIDPRYFPFKSWYNEYLKFASRQDVE
                     EFMSKYPFDILHLQYMDLHLYPYFLYHPDWKVAFYGTGGIVFVKKHIPLPGNTFQYSN
                     YIYSYPSLTQSVLILNFLARTLNWQGCELLLQHMQQEFTWSNQKPLVEAANTMYQGVR
                     AFYRRDYAKANELFENTKGRAPIPANILSTLYHYKAVDLWTQNKIYDALDYTTRVLEL
                     NNYDEPYATFNIGVINWYIYKQYQQQGITGFITQTPVQKNWMEPLQQFLKQTAGNPKA
                     KPARDIANAIINGTYSGDKPPLLYPPEPAYMP"
     gene            complement(3217..3513)
                     /locus_tag="PLX22_11080"
     CDS             complement(3217..3513)
                     /locus_tag="PLX22_11080"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT20480.1"
                     /translation="MDQTIVAKAKQYASIVRRTMPVKRIVLYGSYAKNSENSSSDIDI
                     AVIVDKFNGDYLELSAKLFELVQKVDVRIEPILLNENSDTSGFIESILKYGKEI"
     gene            complement(3907..>4151)
                     /locus_tag="PLX22_11085"
     CDS             complement(3907..>4151)
                     /locus_tag="PLX22_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20481.1"
                     /translation="TQEVKTNIEEFLRQSEIKAKELSLIERVIRVEEELKALKEIEKA
                     RFEAMEKRFTTLQWFIGIGFTVISILIAVFGLFVGQ"
BASE COUNT         1182 a          786 c          848 g         1335 t
ORIGIN      
        1 tattactatc ataggcaact atctcaatgc atgcaagccc gatgtttcaa tactggagat
       61 tggcaccggc atggggattg ctgctgcctg tcttaaaaag gcaggttttg atgtgcatgc
      121 cattgaaccg cataccgcag gctttgaaaa tcgttccctg ctgtatcaaa gggtgtatgc
      181 tcttttagac ataggtgata ttgttttgcc ggtaactatc gaacagatgc ctttgaacaa
      241 acaatacgat tttatttttt catttaatgt gcttgaacat gttaacagga ttgaagctgc
      301 tttcagggta ctatcgcaca tactaaaacc cgctggcgtt atggttcata gctgtcccaa
      361 ctattacata ccgtatgaac cgcattgcaa tgtgctgctg ctgccttttg tgccgcagta
      421 tacacactat atttgcagac gcctgcgcaa tagtgtgggg ctatcgttaa catttaccac
      481 ggtggggtta ataaaaaagc ttgccaaaac caataacctt gcggtaacct ttaaaaacga
      541 tgtgctgtac aatgcgttta ggcgttttga gtatgatgca gcgtttgcac aacgtcaaaa
      601 ggggctgccg ctggtggtgt ataaattgtt aaaaaaatgc aggatactgt ggctggcaaa
      661 gcatgtacct gttgttatgc aaacacccat ggtatttgag ctgaaaaaaa agtaggctat
      721 ggcatatacg caggctcagg ggggtacaaa agcggcggct tatcaccgct ataggtgccg
      781 tttatgatgg cgttggcaat atcgcgggcg ggctttgcct ttgggttgcc ggcggtttgt
      841 tttaaaaatt gctgtaacgg ctccatccag tttttttgta ccggtgtttg cgtaatgaag
      901 ccggtaatgc cctgctgttg atattgtttg tagatgtacc agtttatgac gcctatatta
      961 aaggttgcgt agggctcatc gtaattgttt agctccagca cccttgtggt atagtcaagg
     1021 gcgtcgtaaa ttttgttttg cgtccataaa tcaaccgctt tatagtggta cagtgtactt
     1081 aaaatattgg cggggattgg tgctctgcct tttgtatttt caaaaagctc attggctttt
     1141 gcatagtcgc ggcggtaaaa ggctctgacc ccctgataca tggtgtttgc cgcctcaacc
     1201 agcggctttt gattggacca tgtaaattct tgctgcatat gctgtaacaa aagctcgcaa
     1261 ccttgccagt ttaatgttcg cgctaaaaag tttagtatca gtactgattg cgttaagctg
     1321 ggatagctgt agatgtaatt gctgtattgg aaggtattgc cgggaagtgg tatatgcttt
     1381 tttacaaata ctatcccgcc cgttccgtaa aatgccacct tccaatcggg atgataaagg
     1441 aaataagggt atagatgtaa atccatatac tgaagatgca gtatatcaaa ggggtatttg
     1501 ctcatgaact cttcaacatc ctgccgggat gcaaatttaa ggtattcatt gtaccagctt
     1561 ttaaacggga agtagcgcgg gtcaatcatg atcttattgt ccgggtacat gcgccacaaa
     1621 aagtagcctc cgcagttgta gggattgcca atggtatagc ccggatagta ctttgctata
     1681 aaatttgctt cttcatcgct gttaataaat gaattgccaa atccaaacca tgttgcattg
     1741 gcggtaaagt tgataaaggt atgccagcgc aactggtcca accccgctaa cagcatgatg
     1801 gcggtaatga tggctggtat ccaccttttt tttggtgtat agtaccacgc atcccctttt
     1861 atataaaaca gcgtgctgaa tacatacacc ggtatccagt agtagataag ccttccaaat
     1921 atggtgtata aagccgcata catcacattg tttacaataa acacccagtc aattgtatgt
     1981 aaacggtagc gtttggtgct tataatggta tatagcagta taataatggg gataacaaaa
     2041 tacagcacaa aggttggtac ggcaagggta ggtgtatatg cctgcactgt tttaaggttt
     2101 tcaacatcag gctttagtaa aatcgactgc agcaaatcaa ggtgatagct gtatccatac
     2161 ggtgttacaa atgttgccaa aaatgacaat atccatgcaa tggtaatatg ccgtaatact
     2221 tttttattga ccgttgtatc cttttgaaat ttataattta agagtatgcc tatataggtg
     2281 cacacaaaaa atggtgcagc aaagataaaa ccgccgtggc tatttaccca tataaggatg
     2341 acaatcggaa tgccataaag gtatgcaagg tttttttgca tggtaaggct aaacatgtac
     2401 cataaaaata ccatgatggt cataaacaca taggtaaaaa gctccggctt tatcatactt
     2461 cccgacgagt tgccgtgcgt tataaccccc attaaaataa caaacaggct tacaggatgg
     2521 gttagcacat ttcttttgat tgcatacagt ataaacagcg caagcgcaaa aacagccagt
     2581 gcgtatttta ctgcatacag gctgtaaaat ccatatgctg agtacagtgc atagagtata
     2641 atctcaggta tccatgcgca gtacaccgtg tcgttgcttg ccggtgtcca tgtgtacagt
     2701 gagtggtgtg ggattagcgt tttatgttcc agcatgtatt gtccatactc catgtgccac
     2761 catgtgtcgc catcatcaat ggggcgggtg ttaccaatga ataaggcagc agcaataaac
     2821 agcaacagta tgacggttac tactatcttt gcaattttca tgatgtgtac cgtattaaca
     2881 tggattttta tgccggtgaa aatgatagta tatgccgggt atactttttt ttagcaagca
     2941 ttttattgtt ctttcttttt ttttgggcga tagttaccgg gtagatagta tcataaatga
     3001 tgtaatgtac agtaactatt ggctatttgt taaaaaaagt atataaagta tataaagtat
     3061 atattgaatc aattactggt tttaattcat catttgtcat tccgcaatat agcggcagtc
     3121 gtaataatcg ttgactttct tgtgttgtga actggtcaat ctactataat gtgaaaactt
     3181 tatcatcttt ttcttattgc ttcaaatttg actttatcaa atctctttcc catatttcag
     3241 aatgctttca ataaaaccac ttgtatcaga attttcatta agcagaatgg gttcaatgcg
     3301 cacatcaacc ttttgtacta attcaaaaag ttttgcactt agttccagat aatcaccatt
     3361 aaacttatca acgataactg ctatatcaat atcgctgcta ctgttttcag aattttttgc
     3421 gtatgatcca tacaaaacaa ttcttttcac aggcattgtt cgtctaacta tgctggcata
     3481 ctgctttgct tttgcaacaa ttgtttgatc cattcaaata actcctcggt ttttttgagt
     3541 agatcattac aaacctgctc agtgagtgct tttattaatt cgtttttgtc ctgtggatag
     3601 cgtgctaaaa tattcaaagg cattaaataa tttaaaagcg tgttcatatc ttcattcata
     3661 tactgtttta attttatata aaatattatt ttatgtcaag cagattacaa taggtgttaa
     3721 ttaatacaat ggaatgctat agttactatg caaacaaagt tttcccttaa tgaactttga
     3781 gtcatcatta gctatttgtt aaaaaaagta tatactgcat ttactccatt gttatttaag
     3841 tattcaatca atgcgctgcg cgggtttata tcccttactg agttattaag gctttgcgta
     3901 tggtatctat tgcccaacaa aaagaccaaa cacagcaata agtatgctga tgacggtaaa
     3961 gccaatgcct ataaaccact gcagggtggt gaaacgtttt tccattgcct caaagcgtgc
     4021 tttttctatc tcttttaatg ctttcaattc ctcttctaca cggataacac gctcaatgag
     4081 cgataattct ttagccttga tttcactttg acgcaaaaac tcctcgatat tggtttttac
     4141 ctcctgtgtt a
//
LOCUS       DAOSOX010000204         4138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21643_length_4138_cov_7.663728, whole genome shotgun sequence.
ACCESSION   DAOSOX010000204 DAOSOX010000000
VERSION     DAOSOX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21643_length_4138_cov_7.663728"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      14..89
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            249..2231
                     /locus_tag="PLX22_11090"
     CDS             249..2231
                     /locus_tag="PLX22_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOT20482.1"
                     /translation="MLKINNCFISLLFFLSIAPAIYAQDNGSTPDNLTGIIEAVAPST
                     NAQTGEVALDDVIISAYKSNITYAESASVAVITQKDIEKGGYRFVSDVLQSVPGLQLV
                     QSSTLGGTASVFIRGAKTGNMVVLIDGVKVNDISTIERTFDFATLATDSIERIEIIKG
                     PQSALYGSDATGGIINIVTKKGKGKPAVTLNMYAGSYYTTQQSANISGGDSSLNYSLT
                     AGQISAQGYSKAAKPKNAAAPFDDDGFYQRYATGKFGFTPVAPLLIDAGFTVKKSTIE
                     VDDSSYTDDPNSDEERTELSGYTTIKHTIASFWEQSVTVNASRTQREYYDYNDGAWDW
                     PADNTYTGSLYSAEWLQVFSLKNVDTLTVGVNYEKELSHIKDYLNGTSTNNDNSTTGF
                     FAQNHINAGNILYVTAGVRADNNEIFGWQQSYTVSPAIVIPKIKTKLKANYGKGYKAP
                     SLYQIYGDGGSIVVENKDLKPEENTGYDIGIEQPLLSIATVNLTYFHNKYKDMIDYDS
                     SSYPGRYININRVKTYGYESSVTITPLKALTLSGGYTYLKTEDETTDKPLMRRPKHQV
                     YSSLTVTIAKFEICATGTYIGKRKDAYYDSATYSTVDVTLDGYFKADIAAHYALTPQL
                     TIHAKAENITDKKYQQTCGYAMPQRSYYVGLTAMVQ"
     gene            2336..2587
                     /locus_tag="PLX22_11095"
     CDS             2336..2587
                     /locus_tag="PLX22_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HOT20483.1"
                     /translation="MAKNTIHDRVYKADIIYRLQLDDTDIYLLIKFPLTVDKFMTFRM
                     LTYVMGLYRELIYNHKLTRLPVTFPLVYRGDAQWAVAPS"
     gene            2944..3102
                     /locus_tag="PLX22_11100"
     CDS             2944..3102
                     /locus_tag="PLX22_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20484.1"
                     /translation="MHGMTKERLDIAKKMSRNGIDDAIIKISKISPDELQTMKKKMNS
                     DYTIWRKS"
     gene            3099..3461
                     /locus_tag="PLX22_11105"
     CDS             3099..3461
                     /locus_tag="PLX22_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20485.1"
                     /translation="MTQYRIITIIIASLIVTAGIGCTNDDTTELAWFNAAGNPINDII
                     WANDDQQWTTENGYADQTKTESKKVNALYGIVIASIDEGSGFREGTVIIKETSSSSLS
                     LHEGSSEVYTIDSVSVDP"
     gene            3501..>4138
                     /locus_tag="PLX22_11110"
     CDS             3501..>4138
                     /locus_tag="PLX22_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20486.1"
                     /translation="MKQYQTIVLVLTVCLIGIGTTLLQASDFIWQQISGNWSVVTDNK
                     ESYLKENRSKAFNFDYSPLINCNAIISTNSAIVFTTVTHTLTLDAPQGTATYLTFFNA
                     SDYRQFYAVQFTGNDSCITTISIIQSDIKDTTLPKQAKGNFIITTLASSDVHLAYGQP
                     YTLTITINNTSIAVLVDNKQVLSHTLNEKLEGGSIGFASKNCIPIIDNCKVY"
BASE COUNT         1339 a          833 c          865 g         1101 t
ORIGIN      
        1 aaaatcagta gacaaggagg aaggtgggtg caaatcccac gctgacccgc aactgtgaac
       61 ccattatatt ttgggtaagc cagatactcc tttagctgga attccccgag gattgggaac
      121 cagttagtat caaagcaatt tgatcctaac taatgtggca tgcctcgggc atgctttttt
      181 atttttagct tattggtttc cctcctcctc attccagaca tacactttta aactgtgagg
      241 aggtttcaat gcttaaaatc aacaattgtt ttatttcact tttatttttt ttgtcgatag
      301 ctcctgccat atatgcacag gacaacggtt caaccccgga taatctgacg ggtattattg
      361 aggcagtagc cccttccacc aatgcacaaa caggcgaggt tgcattggac gacgttatca
      421 tcagtgctta caaaagcaac atcacctatg ctgaaagcgc ctcggttgct gtaataacac
      481 aaaaggatat tgaaaaggga gggtatcgct ttgtttcgga tgtgcttcag tcggtgccgg
      541 ggctacagct tgttcagtca tcaacattgg gtggtactgc aagcgttttt atccgtggtg
      601 caaagaccgg caacatggtg gtattgattg atggcgtaaa ggttaatgac atatcaacta
      661 tcgagcgaac atttgacttt gctacccttg caaccgactc tattgagcgc attgaaatta
      721 ttaaaggtcc gcaaagcgct ctgtatggtt cggatgcaac cggtgggatt atcaacattg
      781 taacaaaaaa ggggaaaggc aaaccagcgg taacacttaa tatgtatgca ggttcatatt
      841 acaccacaca gcagtcggca aatataagcg ggggcgatag ctcattgaat tattctttga
      901 ccgcagggca gatatcagca cagggttatt caaaagcagc aaagccaaaa aatgctgcag
      961 caccttttga cgatgatggc ttttatcagc ggtacgccac gggaaaattt ggatttaccc
     1021 ctgtagcccc attattgatt gatgctggat ttaccgtaaa aaagtcaact attgaggttg
     1081 atgactcttc gtatactgat gatcctaaca gtgatgaaga gcgtaccgaa ctttcgggct
     1141 acacaaccat caagcatacc attgcatcgt tctgggaaca aagtgttacg gtgaatgctt
     1201 cgcgaacgca gcgtgaatac tatgattata atgatggcgc atgggattgg ccagcagaca
     1261 atacttacac cggctcacta tacagtgcag aatggctgca ggttttcagc ctcaagaatg
     1321 ttgatacatt gaccgtggga gtaaactatg aaaaggaact atcgcacata aaagattatt
     1381 taaatggcac atcaacaaac aatgataaca gcaccaccgg cttttttgcg caaaatcata
     1441 tcaatgcagg caatatactg tatgtaactg caggggtcag agccgataac aatgaaattt
     1501 ttggctggca gcaaagctat actgtttcac ccgccatcgt tataccaaaa ataaaaacaa
     1561 agctcaaagc aaattacggc aagggataca aagcgccatc cctgtaccag atttatggcg
     1621 atggcggcag tatagtagtt gaaaataagg acctgaaacc ggaggaaaat accggttatg
     1681 atattggcat tgagcagcca ttgttatcga tagcaactgt taatctgact tattttcaca
     1741 acaaatacaa ggatatgatc gattacgatt cttcatccta tccaggaagg tatatcaaca
     1801 ttaacagggt taaaacctat ggttacgaaa gctcggtaac aataacgcca ttgaaggctc
     1861 tgaccctatc gggcggatat acctatctta aaactgagga tgaaacaacg gataaaccat
     1921 tgatgcgaag gccaaaacat caggtgtata gctcattaac agttactatc gccaaatttg
     1981 agatatgtgc aaccggcacg tacattggta aacgaaaaga tgcttactat gacagtgcta
     2041 catactcaac ggttgatgta acactggatg gttactttaa ggctgatatt gcagctcact
     2101 atgctctgac ccctcagcta accatacatg caaaagctga aaatatcacc gataaaaagt
     2161 atcagcaaac atgcggctat gcaatgcctc agcgcagtta ctatgtgggg cttacggcta
     2221 tggtgcaata aaacttttta tataattttt tacatttttt ctcatgtact ttttaatttt
     2281 ttctatcgca agcgtattaa tagatagcac ataactatat agtggcgggg catcgatggc
     2341 aaaaaacact atccacgaca gggtatataa ggccgatatt atttacaggc ttcagcttga
     2401 cgataccgac atataccttt tgattaaatt cccgttaaca gtggacaagt ttatgacttt
     2461 taggatgctc acctatgtga tggggctata ccgtgagctt atctataacc ataagctcac
     2521 gcgcttgccg gttaccttcc cattagtgta ccgtggcgat gcgcaatggg cagtagcacc
     2581 atcatgaacc gtatttttac tggaaaccag tgataaacag cagtttttca aaacacttga
     2641 caaaatagaa gaactgctgc atcgttataa caaagagccg tatatactgc agacattgtt
     2701 gacatggttt atgcattatt tgcacactca tggtataatt aacacaatac cagagatata
     2761 ccagagattg aagagcattt atcaaacgtt caggaagcac aaacgatatt atatacatca
     2821 ctgcaaaaaa taaatgaaga ttataaaaga gaaggatatc ctgaaggaca tcagcaaggc
     2881 agagaagaag ggattaagaa agggttaaaa gcgggtatta cccagggcaa acaacagggt
     2941 ttagtgcatg ggatgacaaa agagcggttg gacattgcaa agaagatgag cagaaatggc
     3001 attgatgatg cgataataaa aatatcaaag ataagtcctg atgaattaca aacaatgaaa
     3061 aagaagatga actctgacta tacaatctgg aggaaatcat gacacagtac cgtataataa
     3121 ctataataat agcaagcctt atagtgacag ctggcattgg ctgtactaat gatgacacaa
     3181 ctgagctggc atggtttaat gctgctggta atccaataaa tgatattata tgggcaaatg
     3241 atgaccagca atggactaca gaaaacggct atgctgacca aactaaaact gagtcaaaaa
     3301 aggttaatgc attatatgga attgttatcg caagcatcga tgagggctca ggattccggg
     3361 aaggaacggt tattattaag gaaaccagta gcagttcgct cagtttgcat gaaggttctt
     3421 ctgaagtata tacaatcgat tcagtttcag tagatccatg atttttttta aaataagcat
     3481 atgaacaaaa tagtgtcacc gtgaaacaat atcaaactat cgtacttgtt ttaacagtgt
     3541 gcctgattgg cattggcacc acgctattgc aggcttcgga ctttatatgg cagcaaatat
     3601 cgggaaactg gtcggttgtt actgataaca aagaatccta ccttaaagaa aaccgcagca
     3661 aagcattcaa ctttgattat agcccgctta ttaattgtaa cgccatcata tctaccaata
     3721 gtgctattgt atttaccacg gtaacgcata ccctaacgct tgatgcaccg cagggcactg
     3781 ccacctatct tacctttttt aatgcatctg attatcgcca gttttatgct gtgcaattta
     3841 ccggcaatga ttcctgcatc acaactatta gtattataca gtctgacata aaggatacaa
     3901 cactgccaaa gcaggctaag ggcaacttca tcatcaccac acttgcctcc agcgatgtgc
     3961 atcttgctta cgggcaacca tatacgctga ccatcactat aaacaacaca tccattgcag
     4021 tactggtgga taataaacag gtactatcgc acacgcttaa tgaaaaactg gaaggtggca
     4081 gcattggctt tgcatcaaaa aactgcattc ccatcattga caattgcaag gtgtataa
//
LOCUS       DAOSOX010000205         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_21872_length_4103_cov_10.051877, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000205 DAOSOX010000000
VERSION     DAOSOX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21872_length_4103_cov_10.051877"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1091)
                     /locus_tag="PLX22_11115"
     CDS             complement(27..1091)
                     /locus_tag="PLX22_11115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT20487.1"
                     /translation="MEDNGLTFFEETELTPETFAGLEKKVYDLRNLLEMGISLSSNLQ
                     FESLVESLLYSCIGQMFVEQVALLLQKDIDVNDYYIHMTKGYDTECKNGIVLLETSPL
                     IGFLANNPYPIEYETLLELPDIVEDLKAIAFLNPYIVVPLKSKDSLMGILLLGKKISG
                     EGFTNSEKEFLHYVARFGAVAVENSRLYLMSTLDRMTRLYIHHYFEIRLIEEMKRSQR
                     YNVPLSLLISDIDHFKRFNDTYGHVQGDSVLKEVAQLFLKDLRKMDIPSRYGGEEFAV
                     ILPQTDLEQAGIVAQRLRKLIEQHQFKGQEDALHVTISIGVAQYDPNKDKDTKGFIAR
                     TDKALYKAKQMGRNRVALFK"
     gene            1293..1727
                     /gene="dut"
                     /locus_tag="PLX22_11120"
     CDS             1293..1727
                     /gene="dut"
                     /locus_tag="PLX22_11120"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT20488.1"
                     /translation="MITIKITLKHGAKKPDYVTHSASGADIYTFLDKPVILQPFQRVL
                     IPTGLSIELPPGYEAQIRPRSGLAINHGITLLNTPGTIDADYRGEIKLIVINLGEKPF
                     TIENNMRIAQMIIAQTVPATFTVVAELSDTKRNDGGFGHTGL"
     gene            1724..3370
                     /locus_tag="PLX22_11125"
     CDS             1724..3370
                     /locus_tag="PLX22_11125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT20489.1"
                     /translation="MKEKKIRIIPIGGLHAIGGNMLVIEYDNEMLIIDCGISFPNGET
                     PGIDFMIPDFTYILENKHKVKGIIITHGHEDHIGAIPFFLQKINVPVYATKLTLGLVA
                     SRLAERPVSQHTFIEIEPRDTIHIGPFIIEFLLVNHSIIDGVGLAIKTPLGIIIHTGD
                     FKIDYSPVDGKVADLHRFAEYGQQDVLLLISDSTNATKPGYTPSEIILKKKLLEIFAR
                     SKGRIFVASFASNIHRIQQVLEASQKYNRKVVISGTTMQKNVEIATELGYLQYKKDLI
                     IDAKDTGKYTHKKLVVLCTGSQGEPMSALHRMANGTHKHFRISAGDTVVITASVIPGN
                     ERMVYNVINSLMQLGALVYYEQDEDIHVSGHASQEELKLMITLTQPKYFLPVHGEYRH
                     LKAHAALAESLNIKPKDIIVAKNGDIIELTDKSFEKRGSIPLSTVYVDGNYIGDITDE
                     VIAERKTLSSDGVIVISIVIGDGLLLYPPAIIAKGFIGLKNDKFLQVLQKELSATIEK
                     HLAQNASSENISIAVKKLVKMIAHRFTNNTPLVETIIQEI"
     gene            3372..>4103
                     /locus_tag="PLX22_11130"
     CDS             3372..>4103
                     /locus_tag="PLX22_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT20490.1"
                     /translation="MAYVIVMFCVLGIIQGITEFFPVSSSGHLALLESIPYFNSIFSG
                     MDEGIKLFFNVALHLASLCAIIIFYYHDIVALIKGTIAELYKKNWGPNCRFVLHIIIA
                     SIPAAIIGILFNDYVEATMASPAIVSILLVANGIMLIKANTLTKKNRKCEEMHYWQAF
                     FIGLFQAVAILPGISRSGSTIAGGLMAGLQPEEAGKFSFLMAIPVIAGAGLLESRKLF
                     AIDGIHQIVVPTIIAMVVTFIAALLS"
BASE COUNT         1282 a          819 c          756 g         1246 t
ORIGIN      
        1 attctatacc atttactatc gacattttac ttaaagaggg caactctatt tcttcccatc
       61 tgttttgcct tatataaagc cttatcagta cgtgctatga accccttggt gtccttatcc
      121 ttattggggt catactgtgc aacacctatg ctgatagtta catgcaaagc atcttcctgc
      181 cctttaaatt gatgttgctc aatcaattta cgcaaccttt gcgcaacaat tcctgcctgc
      241 tcaagatctg tctgcggcaa aattactgca aattcttccc caccataccg tgaagggata
      301 tccatcttac gtaaatcttt taaaaaaagt tgagccacct cttttaaaac gctatcaccc
      361 tgcacatgcc cataggtatc attgaatcgt ttaaaatgat caatatcact aattaaaagc
      421 gatagcggaa cattatatcg ttgactgcgc ttcatctctt caatgaggcg gatttcaaaa
      481 taatgatgga tatatagccg tgtcatacgg tcaagtgttg acatcaaata cagacgtgaa
      541 ttttcaacag caactgcgcc aaagcgggca acataatgta aaaattcttt ttctgaattg
      601 gtaaaccctt cccctgatat ctttttccct aaaagcaaaa tccccatcaa tgaatctttt
      661 gatttcaatg gtactacaat ataaggattt aagaatgcta ttgcttttaa atcctcaaca
      721 atatccggca attccaatag cgtttcgtat tctataggat atgggttatt agcaagaaat
      781 ccaattaatg ggctggtttc aagtaacacg ataccattct tacattcagt atcataccct
      841 ttggtcatgt gtatgtaata atcgtttaca tcaatatcct tctgcaaaag aagtgcaacc
      901 tgctctacaa acatctgacc aatacaactg tataataacg attcaaccag gctttcaaat
      961 tgcagattgg aagaaaggct tatacccatt tccagaaggt tgcgaaggtc atataccttt
     1021 ttttcaagtc ctgcaaacgt ttcaggcgtt aattctgttt cttcaaaaaa agttaagcca
     1081 ttatcttcca tgactatccc ttaaattttt cttgtttttt ttacggtacc aatacgttat
     1141 gactctattt atgcatagta tgtcccatgc attgttttgt taaaacaaat tataatagat
     1201 taatttctaa aaaaattata tactaattaa aaaaaatgtc aacaattata tcatggcatg
     1261 atataattta ttcataattc aatataatta ttatgataac aataaagatt acgctaaaac
     1321 atggtgcaaa aaaacctgat tacgtaacac acagtgcatc aggtgctgat atatatactt
     1381 ttctggataa accggtcatt ctccagccct tccagcgggt attgatacca accggattat
     1441 ccattgaatt gccaccaggc tatgaagcac agatacgacc gcgaagtgga cttgctatta
     1501 atcatggcat taccttactc aacacaccgg ggactattga tgcagattat cgtggtgaaa
     1561 taaaattaat tgttattaat ctaggtgaga aaccatttac catagagaac aatatgcgca
     1621 ttgcacagat gatcattgct caaacagtac cagctacctt tacagtagta gctgaacttt
     1681 cagatacaaa acgaaatgac ggaggctttg gacacacagg cttatgaaag aaaagaagat
     1741 acgaattatt cctataggcg gacttcatgc cattggcggc aatatgcttg ttattgagta
     1801 cgacaatgag atgctcatca ttgactgtgg tatatcattc cccaatggtg aaacaccagg
     1861 gatagatttt atgatccctg attttaccta tattctggaa aacaagcata aagttaaggg
     1921 tataatcatt actcatggtc atgaggacca tattggtgct atcccgttct ttctccaaaa
     1981 aattaacgtt ccggtatatg ctacaaagct tacactggga cttgttgcaa gcaggcttgc
     2041 cgaacggccg gtaagccagc acacatttat cgagattgaa cccagagata ccatccacat
     2101 tggtccattt atcattgaat ttttactggt taaccattct attatcgatg gtgtggggct
     2161 tgccataaaa acaccgcttg gtataatcat tcatacaggc gattttaaaa tagattattc
     2221 tccagttgat ggcaaggttg ccgatctgca tcgttttgcc gagtacgggc agcaggatgt
     2281 gctgctcctc ataagcgata gtaccaatgc aacaaagcca gggtacacac catccgaaat
     2341 aatattgaaa aagaaactgc tggaaatatt tgccagatca aaaggacgta tttttgtagc
     2401 aagttttgct tctaatattc accgtatcca gcaagtgctt gaagcatccc agaaatacaa
     2461 tcgtaaggtt gttatatcgg gtactaccat gcagaaaaat gtggagatag caaccgagtt
     2521 aggatatcta cagtataaaa aggatcttat cattgatgca aaggataccg gtaaatacac
     2581 ccataaaaaa ttggtggtgc tgtgtacagg ttcgcagggc gagccaatgt cagctctgca
     2641 tcgcatggca aatggtacac acaagcattt cagaatcagt gccggtgata cagtggtgat
     2701 aacagcatcg gttataccgg gcaatgagcg tatggtttat aatgttatca attcattgat
     2761 gcagctcggc gctttagtat attatgaaca ggatgaggat attcatgtat ccggtcatgc
     2821 atctcaggaa gaattaaaat taatgattac ccttacccaa cccaaatact ttttacctgt
     2881 tcatggggaa tatcgtcacc taaaagcaca tgctgcgctt gccgaatcac tcaacataaa
     2941 acctaaagat attattgtgg caaaaaatgg tgatatcatt gagcttaccg ataaaagttt
     3001 tgaaaaaagg ggaagcatcc cactttcaac tgtctatgta gatggcaact atattggtga
     3061 cattaccgat gaggttattg ctgaacgcaa aacattatct tctgatggag ttattgttat
     3121 aagtattgtc attggcgacg gcttgctact ttatccacct gctattattg ccaagggttt
     3181 cataggatta aaaaatgata aatttttaca ggttctccag aaagaattaa gcgccaccat
     3241 agaaaaacat ctggcacaaa acgcttcttc agaaaatata tcgatagcgg taaaaaagct
     3301 tgttaaaatg attgcccacc gatttaccaa caacacccca ttggtagaaa ctatcattca
     3361 ggagatttaa catggcatat gttattgtga tgttttgcgt tctgggtatc atccagggta
     3421 ttactgaatt tttcccggta tcatcatcag gacatctggc gcttcttgaa tccatcccat
     3481 attttaattc catcttcagc ggcatggatg aaggaataaa actttttttt aatgttgcac
     3541 tccatttagc aagtttgtgt gcaattatca tattttacta tcatgatatc gttgccctga
     3601 ttaagggaac tatcgccgaa ctatataaaa aaaattgggg gcccaattgc aggtttgttt
     3661 tacatattat catagcatca atccctgcag ctattattgg catactgttt aatgactatg
     3721 tagaagctac tatggcaagc cccgctattg tgagtatact tcttgtggca aacggcatca
     3781 tgctgataaa ggctaataca ttaactaaga aaaaccgaaa atgtgaagag atgcactatt
     3841 ggcaggcgtt tttcatagga ttgtttcagg ctgttgctat tttacccgga atctcacgct
     3901 cgggaagcac cattgcaggc ggtttaatgg caggattaca accagaggaa gccggcaaat
     3961 tttcattttt aatggccata ccggtcattg ccggcgcagg gcttcttgag tcacgaaagc
     4021 tctttgccat agatggtatt caccagatag ttgtgccaac cattatagca atggttgtta
     4081 catttattgc agcactgtta tcg
//
LOCUS       DAOSOX010000206         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_22122_length_4069_cov_8.200548, whole genome shotgun sequence.
ACCESSION   DAOSOX010000206 DAOSOX010000000
VERSION     DAOSOX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22122_length_4069_cov_8.200548"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1792
                     /locus_tag="PLX22_11135"
     CDS             <1..1792
                     /locus_tag="PLX22_11135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT20491.1"
                     /translation="STVVSLLSSEAEKKGITLKLECGDVPIIIADRKRLRQVLINLVG
                     NAIKFSDGGTVTVTIQPKDDRGVRIEVCDEGRGIDTKDLDRIWNPFVQAEDPDIRVAG
                     GTGLGLAISKNLVELHGGSIRVESELGKGSRFIVDLPESPGMRGISRRRVEKTQDIVT
                     IKKDVRFESDEKENNTLAQLQSFASARILAVDDDEVNLRVMKGFCDVAGYEFISATNG
                     HEALEILATQDVDIVLLDLMLPGISGYEVCKKIREDAKTMGIPVIMVTAKDTTTDLVR
                     GFITGANDYITKPFSRDELLVRIENQLAIKQMLDAERSIIRELRTESEGHENLYRRSV
                     DMKQMTLQMVAWERLIRDDMEIARAFQDRLMSHHRNVPGIETYVYYRPLMKLGGDIYD
                     IIEVRPGVLRVFLADATGHGITASLNTVKILSEYSSVKQVMQTPEEILNFLNHRFIEL
                     YRDYKIIFTCIIADIDIVHGELSLASAGHPQSYLLSEGKVTPLKPQGPIICLSSNFEY
                     RQMVMPINNRDLLIFYTDGLLELIGSQYRGAEGPGFDTGKLLEEQIASISKSNSLEEI
                     CKSFVAGAESVSTTMNRGSDDITIVAIRKL"
     gene            1864..1947
                     /locus_tag="PLX22_11140"
     tRNA            1864..1947
                     /locus_tag="PLX22_11140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1898..1900,aa:Leu,seq:cag)
     gene            complement(2182..2613)
                     /locus_tag="PLX22_11145"
     CDS             complement(2182..2613)
                     /locus_tag="PLX22_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT20492.1"
                     /translation="MPLFDEKVKTQLAQLLSKLTNPITLHFFTQEIECPTCQTTRQFV
                     EEIASLSDKISLATYDFARDEEARKEFNVDTIPAIIISDKDKAVKGVRFFGIPAGYEI
                     NSFIGACLEVSGAKEPLPQAIIDRIKKIAKPIHIQVFVTLT"
     gene            complement(2744..3727)
                     /locus_tag="PLX22_11150"
     CDS             complement(2744..3727)
                     /locus_tag="PLX22_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT20493.1"
                     /translation="MEITFNLPGLETIEQRTVPLDSSVIYDVIIIGGGPAALTAAVYC
                     MRKGVKTGLVTLDVGGQVAETSVVENYMGYNHIEGISLVQRFKEQVEQFEIGFLQGYK
                     AIQLQDGTIKKVTCEDGNTYMAKAVIITTGKRWRRLNVPGESELTGKGVAYCAICDAP
                     LFKDKDVIVVGGGNSAVEAAIDLAKLATSVTVVQLLPHLTADKVLQDELSKFNNVLIL
                     FEHEVRTIHGTQSVDAVTLYDTKNKKETKSKVQGIFIEIGLIPNTELVKDVVALNHND
                     EIIIDCNCRTSRPGIFAAGDVTSVPYKQIIIAAGEAAKAALSACEYVLLKK"
BASE COUNT         1215 a          776 c          862 g         1216 t
ORIGIN      
        1 ctcaactgtg gtgtcgcttc tttcttcaga ggcagagaag aaaggaataa cgctgaaatt
       61 ggagtgtggt gatgtcccaa taattatagc cgacaggaaa cgcctgaggc aagtacttat
      121 caatctggtt ggtaatgcca taaagttttc tgatggcggg acagtaaccg taacaataca
      181 gcccaaagat gacagaggag tacggattga agtttgcgat gaaggcaggg gaatagatac
      241 aaaggacctt gaccggatat ggaacccctt tgtgcaggca gaggatcctg acattcgcgt
      301 tgctggtggt acgggacttg gccttgcaat ttcaaaaaac ctggtggaac tccatggggg
      361 aagtatacgg gttgaaagtg aattagggaa agggagtaga tttatagttg accttcccga
      421 aagcccggga atgcggggga taagtagaag gcgggtagaa aagacacaag acattgttac
      481 tataaaaaag gatgttcgtt ttgaatctga cgaaaaggaa aataatactc ttgcacaact
      541 gcaatccttc gcttccgctc gcatccttgc cgttgacgat gatgaggtta atctgcgggt
      601 aatgaagggc ttctgcgatg tagctggcta tgagtttatt agcgctacaa atggacatga
      661 agccctggaa attctggcaa cgcaggatgt tgatattgtg ctgttagacc tcatgcttcc
      721 cgggataagc ggttatgagg tgtgcaaaaa aattagggaa gatgcaaaaa ccatgggaat
      781 ccccgttatt atggtaacag caaaagatac cacaacagac ctggtgcgtg gtttcataac
      841 tggcgccaat gactatatca caaagccctt tagccgggat gaacttcttg tcaggataga
      901 aaatcagctt gcgataaagc aaatgctgga tgcagagcgc tccataataa gagaactgcg
      961 aactgaaagt gaaggacatg aaaaccttta ccggcgaagc gtggacatga agcagatgac
     1021 cttacaaatg gttgcatggg agcgccttat acgggatgac atggagattg ctcgagcttt
     1081 tcaggaccgg ctcatgtcgc atcaccgtaa tgttcctgga atagaaacgt atgtgtacta
     1141 tagaccactt atgaaacttg gaggcgatat ctacgatatt attgaggtga ggcctggtgt
     1201 tctcagggtt ttccttgcag atgccaccgg gcatggcatt acagcctccc ttaacacagt
     1261 aaagatactt tcagaatatt cgtctgtgaa gcaagtgatg caaacaccag aggaaatttt
     1321 aaactttctc aatcatcgtt ttatagaatt gtatcgggat tacaaaatta ttttcacatg
     1381 cattattgct gatattgata tcgtacatgg cgagctttct cttgcctcgg cgggacaccc
     1441 tcaatcgtac ctgttatcag agggaaaagt gacaccgcta aaaccgcagg gtcctataat
     1501 ttgcttatcg tcaaattttg aataccgtca gatggttatg cctattaata accgtgatct
     1561 actcattttt tatacagatg gtcttcttga attaataggt tcgcagtatc gtggtgctga
     1621 aggtcctggc tttgatacag ggaagctcct ggaagagcaa attgcttcaa taagcaaatc
     1681 taattcgctt gaagaaatct gtaaatcctt tgttgctggt gctgagtcag tgagtactac
     1741 catgaatcgt ggaagtgacg atataaccat tgttgctatc cggaagctgt gaggaataat
     1801 tattttcttt ttttatttga caaaaaacta tagtatacag aatcaattaa ctaatataaa
     1861 aaagccgcga tggcggaatt ggtagacgcg ccaggttcag ggtctggtag gtgtacgcct
     1921 gtgagggttc aaatccctct cgcggcattt ttaataaaac ggtaattttt ctcaaatctt
     1981 ctcaatcata tctataaata cagtaattgg ttgggctccc acaaactcat atgtatcatt
     2041 gataacaact tttggtactg atgaaacatt atgcttgatt gctaattgtg ggaaagttga
     2101 agtttcaacc atgtcggcta tgatattttg attttcaatt gccagcctgt gagcggatgc
     2161 aactgcaccc ggacagtatg gtcaggtgag tgtgacaaat acctgaatat gtatgggttt
     2221 tgctatcttt ttaattctgt cgataattgc ttgaggcagc ggttcttttg ctccagagac
     2281 ttcaagacag gcgcctataa atgagtttat ttcgtatccg gcagggatac caaaaaaacg
     2341 tactcctttt actgccttgt ctttatctga aataataatt gctggtattg tatcgacatt
     2401 aaattctttt ctggcttctt catctctggc aaaatcgtat gtagcaaggg atatcttatc
     2461 agaaagtgat gcaatctctt caacaaactg gcgtgtagtt tgacaggttg gacattcaat
     2521 ctcctgggta aaaaaatgca gcgtaatagg atttgttaat tttgatagta actgcgctaa
     2581 ttgcgttttt accttttcgt caaaaagcgg catagtaaaa cctccttgtg aaacaaattt
     2641 attagcctta gaatataatt ttaacgaaaa ttgtttaact cattatcagg tattacatag
     2701 taatgtaaaa aactccctgc atagggttat taccaaacaa taattatttc ttcagtaaaa
     2761 catattcgca tgctgataaa gctgctttag ccgcttcacc tgctgctata ataatctgct
     2821 tatagggtac agatgtaaca tcgcctgcgg caaaaattcc gggtcgtgat gttctacagt
     2881 tacaatcgat aatgatttca tcattatggt ttaaggcaac aacatctttt accagttcgg
     2941 tattgggaat caacccgatc tctatgaaga ttccctgtac ttttgattta gtctcttttt
     3001 tattttttgt atcatagagg gtaacagcat ctacagattg tgtgccatga atggtacgca
     3061 cctcatgctc aaatagtatc aaaacattgt taaactttga cagttcatcc tgtaaaacct
     3121 tgtcagccgt taaatgtggt aacagttgaa caaccgtaac cgacgttgcc aattttgcta
     3181 aatcaattgc ggcttccacg gctgaattac caccgccaac cactataaca tctttatcct
     3241 tgaacagcgg tgcgtcgcaa atggcacagt aggcaacgcc tttacccgtt agctcgcttt
     3301 cacccggtac attaagtcgt cgccatcgct tcccggtagt tataataaca gcttttgcca
     3361 tataggtatt cccgtcttcg catgttactt tctttatagt gccgtcctgt aattgtatgg
     3421 ctttatatcc ctgcaaaaaa ccaatttcaa actgttctac ctgttcttta aaacgttgca
     3481 ccagagatat gccttcgata tggttatagc ccatataatt ttcaacaaca gaagtttctg
     3541 caacctgccc gccaacatca agggttacaa ggcctgtttt aacaccttta cgcatgcaat
     3601 aaactgcagc agtaagtgct gcagggcctc cgcctatgat tataacatca tagataacag
     3661 aactgtccag aggtaccgta cgttgttcta ttgtttctaa tccgggaaga ttgaatgtta
     3721 tttccatcgt tacctcatgc tccacggtat taactagtta aaaatgaact gctctctacc
     3781 attatttttt ataaaacgaa aaaacatcca tcattactaa aaaatatata aaaatctttt
     3841 ctataggtca aggattaatt ggtttataaa aaataaataa agagtgacta tcgccagaat
     3901 tttgaaaata tagttttttg taaatgaaaa atttaacata gttgtaacat ttacaggagc
     3961 atttccattg aattgttcaa ggtgtgagct atttttggta atcaccacaa taggtatgaa
     4021 gaggtatata tgtgtggaat agtaggcata ataaatactg atagaacaa
//
LOCUS       DAOSOX010000207         4043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_22321_length_4043_cov_5.957623, whole genome shotgun sequence.
ACCESSION   DAOSOX010000207 DAOSOX010000000
VERSION     DAOSOX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22321_length_4043_cov_5.957623"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1081)
                     /locus_tag="PLX22_11155"
     CDS             complement(170..1081)
                     /locus_tag="PLX22_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOT20494.1"
                     /translation="MTNLTHIIEKYFLVWIVVFSLCGYHFPAIFKGLVAYIPLFLGII
                     MFGMGITLEFSDFVHIVKIPGALIAGVIGQFFIMPLVAYLLCIVFQLPPMLAIGVILV
                     GTCPGGTASNVITYLSKGDVALSVALTTTTTLVSPLATPLLTYLYAGTWIQVPVIPML
                     VSILQVIIIPIVLGITIRYAFKEKIKTIITILPSVSVVAIVTIVAGITAGNALALKTV
                     GLLVIGVVMVHNVLGLIMGYLASWLFGFDEKIRRTIAIEVGMQNSGLAVSLAVTHFEA
                     ISALPGALFSIWHNISGSFIAWWWRRH"
     gene            1080..1238
                     /locus_tag="PLX22_11160"
     CDS             1080..1238
                     /locus_tag="PLX22_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20495.1"
                     /translation="MYAFRLLIKKVWGDSYCLIIYSIEQLHGVMNRIVVTPAWNAQSS
                     KKIALPGN"
     gene            complement(1220..1291)
                     /locus_tag="PLX22_11165"
     tRNA            complement(1220..1291)
                     /locus_tag="PLX22_11165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Arg,seq:ccg)
     gene            1500..1643
                     /locus_tag="PLX22_11170"
     CDS             1500..1643
                     /locus_tag="PLX22_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20496.1"
                     /translation="MQANGERQKEIAKVLGVTERTVRICARAMSCAETTEEGLGIFAF
                     SDY"
     gene            complement(2011..2154)
                     /locus_tag="PLX22_11175"
     CDS             complement(2011..2154)
                     /locus_tag="PLX22_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20497.1"
                     /translation="MRRTIIKLLEYGWPDRIIASYLMIKPAIVKYYRKKYRIIRNEYQ
                     GFY"
     gene            2382..2744
                     /locus_tag="PLX22_11180"
     CDS             2382..2744
                     /locus_tag="PLX22_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT20498.1"
                     /translation="MARTNKFNVEDNIVNSIIGEGSEFKGEFTINGLLRIDGKFKGTI
                     ATDGKVLIGQTGEAITDIKARVVVIGGTIKGNIFASERVIMLSTGFIQGNVITPSLIM
                     EDGVTFEGNCIINKEQDV"
     gene            2741..3181
                     /locus_tag="PLX22_11185"
     CDS             2741..3181
                     /locus_tag="PLX22_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HOT20499.1"
                     /translation="MIEIVPPNQKKNEQKLVKQKKGSKTAGASSSFRTTFEQTIEFSL
                     PETMEELLNNLKDQEKNFLSDPSMYNMQRYKALIQKIVKILVSEEYTVNVLQRKRRDR
                     ADFIVIQKIENKLEAITNAYIKNEAFNFLKTFDEIRGLILDLKH"
     gene            3187..>4043
                     /locus_tag="PLX22_11190"
     CDS             3187..>4043
                     /locus_tag="PLX22_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOT20500.1"
                     /translation="MQPLSGNHKQQTIISHCLHNASLPHSLLFSGLEGIGKKTFAMTL
                     AKMMFCKNNACNTCPSCLQIEHGIHPDVIVMGPNDKGTIPIGNDDEHEGSIRWLIARI
                     SLAPVNNCRVVIINDAHTMNMQAQNALLKTIEEPPHNNFIILISSERNQLLPTIRSRI
                     TEYVFNPLSQEELTAILVHNGVSHEKHNAILAVASGSASYALILSDDTLYAMIVDAYR
                     AIVNFVIHPEVFSHDLLPLLQALGPYKTIDLLINIFRLHCGNSFPSLPWFDSYLKEET
                     AVEIIKILL"
BASE COUNT         1339 a          795 c          737 g         1172 t
ORIGIN      
        1 ctcaccggtc atgttgacaa cttgcagaaa acattaggtg aataagtact ctataatgtt
       61 ctgctaccga tttggcactc actttttatg cttgacgttt tcctcttaat tcactaaatt
      121 atatatattt tggtggatgt attcacaatt attatattat tttgcttatc taatgtcgtc
      181 tccaccacca cgcaatgaac gagcccgaaa tgttgtgcca tatactgaat agtgcacccg
      241 gcagcgcact gattgcttca aaatgggtaa ccgcaaggga caccgcaagt ccggaatttt
      301 gcatacccac ctcaatggca atggttcgcc ttatcttttc atcaaagcca aataaccagc
      361 ttgccaggta acccataatc agtcccagca cattgtgcac cataacaacg cctatcacaa
      421 gcagcccaac tgttttcagg gctaaagcat ttccggcagt aatacccgct acaatagtaa
      481 ctatcgccac aaccgaaaca gaggggagta tggttataat agtttttatt ttttctttga
      541 acgcatagcg gatggttatt cccagaacaa ttggtataat aatgacctgc agtatggaaa
      601 cgagcatggg tataaccggc acctgtatcc atgtacctgc atacaggtag gttaacagcg
      661 gtgtagctaa cggtgaaaca agtgtggtgg tagttgtcag tgcaaccgaa agcgccacat
      721 cgccttttga caggtaggta ataacattgg atgctgtgcc gccggggcat gttccaacaa
      781 gtataacccc aattgcaagc ataggaggta attgaaatac tatgcacaac aggtatgcaa
      841 caagaggcat gataaaaaat tgacctatca ctcccgcaat aagtgcgccg ggaattttta
      901 ctatgtgtac aaaatcagaa aattcaagtg ttatgcccat cccaaacatg ataatgccta
      961 aaaacaatgg tatatatgcc acaagccctt tgaatatagc agggaaatgg tagccacata
     1021 gtgaaaaaac cacaatccat actaaaaaat atttttcaat gatatgcgtt aggtttgtca
     1081 tgtatgcgtt ccggttactt ataaaaaagg tatggggcga tagttactgt ttaataattt
     1141 atagcataga acaattgcat ggtgtaatga accgcatagt ggttacacca gcatggaatg
     1201 cacagagcag taaaaaaatc gctctccccg ggaattgaac ccggacacac ggctccggag
     1261 gccgacgctc tatccgttga gctaggagag ctgtataaga aaccgtttct atatagtaaa
     1321 agccacggtt acctgtcaag ataatttatg gtaataccgt gctgtcaaca ccgatttaaa
     1381 attgccgctt tttagtataa taaaaagggg tcaaccccga agatgggatg tgttacaaaa
     1441 ccatcaacaa gggagacccc ataatgaaaa aggagtagaa aatcatggat gcaaaaatta
     1501 tgcaagcaaa cggtgaacgg cagaaagaaa ttgcgaaggt actgggtgtg acagaaagga
     1561 cagtacggat ttgtgcacgg gcgatgtcat gtgcggaaac cacagaagaa ggactcggca
     1621 tatttgcttt ttcagattat taatgcacga tatgaactag atgattacaa aggattaaaa
     1681 ccaaaaataa acaatccatt tttaatgtaa caggtctatc taataacttc aaaatataaa
     1741 tataaatcta tacctataca aaatagaaaa aatttgcatt acaggttata tatacacaat
     1801 cttttgattt ttttaaagga ttaatacaaa atttttttgt ttttttgccg atataagcta
     1861 ttaatagtgt tgacaagtaa gcagcaaaat ttttttggtg ctatactttc ctccaattta
     1921 atgattggcg aacctccaaa ttctctttag aggggaatcc tcttcccctc tactttttat
     1981 agtgttgtta tgtaaagatc tgacattgta tcagtaaaat ccctgatact cgttacggat
     2041 tattctatat tttttcctgt aatacttgac aatggcaggc tttatcataa ggtaacttgc
     2101 aataattcgg tcgggccaac catattctaa aagtttgatg attgttctcc tcatggttcc
     2161 ctccttgaat ttaagcatac tagtccgttc tggttataat ataaccaatt ataaaaattg
     2221 caagcttttt ttaaaaattg tcaaaatttt ttgacactca tatatatggc aaaaatgtca
     2281 taaaattata cttgtcttta taggtataat tttgtaatgt aacatattaa taaaataaca
     2341 aaacaattgc atcagcataa aatcttaaaa aagaagctca tatggcacga acaaataaat
     2401 ttaatgttga agataatatt gtaaacagca taatcggtga aggctctgaa tttaaaggtg
     2461 aatttaccat caatggactg ctccgtattg atggtaaatt taaaggaact atcgccaccg
     2521 atgggaaggt acttatagga caaacaggcg aagctatcac agacatcaaa gctcgtgttg
     2581 tcgttattgg tggaactata aaaggcaata tctttgcatc tgaacgtgtt atcatgcttt
     2641 ctaccggttt catacagggt aacgtcatca ctcccagtct tataatggaa gatggtgtta
     2701 cctttgaagg taattgtatt attaataaag agcaagatgt atgattgaga tagtacctcc
     2761 aaaccagaag aaaaacgagc aaaagcttgt taagcaaaaa aaagggagta aaaccgccgg
     2821 tgcatcatct tccttccgta ccacctttga acaaaccatt gaattttctt tacccgaaac
     2881 tatggaagaa cttttaaata acctgaaaga ccaggaaaaa aatttcctct ctgatccttc
     2941 aatgtataat atgcagcgat ataaagcact gatacaaaag atagtaaaaa tactggtgag
     3001 cgaagaatat acagttaatg ttttacagcg caagcgtcga gacagagcag attttattgt
     3061 tatccagaaa attgagaaca agcttgaagc aataacaaat gcatatataa aaaatgaggc
     3121 atttaatttt ttaaaaacct ttgatgaaat cagaggactt atcctggatc ttaaacattg
     3181 attaccatgc aaccattatc aggcaaccat aagcaacaaa ctattatttc acactgttta
     3241 cacaatgctt ctcttcccca ttccctgctt ttttccgggc ttgaaggtat tggtaaaaaa
     3301 acgtttgcta tgacacttgc caaaatgatg ttttgcaaaa acaatgcatg caatacatgc
     3361 ccctcatgtc tacagattga acatggaata catcctgacg ttattgttat gggacccaat
     3421 gataaaggca ccatacctat tggtaatgat gatgaacatg aaggctctat acgctggctt
     3481 attgcccgca tttcacttgc tccggttaat aactgcaggg tggttatcat taatgatgct
     3541 cacaccatga atatgcaggc tcaaaatgcc ctgcttaaaa ctattgaaga accaccacac
     3601 aataacttta tcattttaat aagctctgaa cgcaatcagc tattaccaac aatacgctcc
     3661 cgtattaccg aatatgtctt taacccgctt tcacaagaag agcttacagc cattctggta
     3721 cacaatggcg tgtctcatga aaaacacaac gccatccttg ccgttgcaag cggttcggca
     3781 tcgtatgccc tgatactttc cgatgacaca ctatacgcta tgatagtaga cgcctaccgt
     3841 gccattgtaa actttgttat acaccctgag gttttttccc atgatctttt gccactgcta
     3901 caggctcttg gcccttataa aacaattgat cttttaatta acatcttcag acttcattgt
     3961 ggcaacagtt tcccttccct tccctggttc gatagttacc ttaaagagga aaccgctgtg
     4021 gaaattatta aaatattact tga
//
LOCUS       DAOSOX010000208         4038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_22359_length_4038_cov_8.128295, whole genome shotgun sequence.
ACCESSION   DAOSOX010000208 DAOSOX010000000
VERSION     DAOSOX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22359_length_4038_cov_8.128295"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLX22_11195"
     CDS             <1..243
                     /locus_tag="PLX22_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT20501.1"
                     /translation="VKSRGNEIALMLTDLVMPGMNGVELYEAINKINPHIKNIFMSGY
                     TDLVIYEHIVNKESNYLQKPFSMFDLAKKLKHVLTS"
     gene            complement(305..1621)
                     /locus_tag="PLX22_11200"
     CDS             complement(305..1621)
                     /locus_tag="PLX22_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20502.1"
                     /translation="MKTKHLIIFLSAIALITSCSSSAKKTDTKTDNTMNGLVKIYWED
                     KSLKGQGNVINGVKTGRWILYHKGSGEKLAEGEYVNDKQQGFWTYYHKNGAKSLEGNF
                     LEDQKTGEWLAYYPDGNIMWKATYVIKEKTVSGFTIKAGTIEGIKIWYYQSGKIWKEE
                     QYTNGEQTGRTQEYYENGTPKIIAWYKAGQLNGQCNKWYENGKKQEEGMYTENKKNGN
                     FKFYFDNGQVATTGSFVMDKPEGDWKFYSKEGLLQKEGKYNNGKEIGLWNFYMYPSRV
                     QRVKIMELPLQGGMIDNTNEKAVLYNEKGQIVGTGQLTGIPKGIYSIIKDGKQTGEIS
                     SSSVPVDNPKEKISYTWTGKWETPIKNGKWTEYYPGTKAIKLEAIYLMDKLNGAYKEY
                     YQNGKIKSEGEYLNGKMNGEWKFYNPDGSIDEAQSGRYMLGKKINF"
     gene            1964..2758
                     /gene="flgG"
                     /locus_tag="PLX22_11205"
     CDS             1964..2758
                     /gene="flgG"
                     /locus_tag="PLX22_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOT20503.1"
                     /translation="MMRSLWTAASGMIGQQFNIDTISNNLSNVNTAGFKKMRAEFEDL
                     IYQTVLMAGTPATEISEVPTGIQVGHGVKVSATQKLFEQGSLQSTGNTMDIALEGEGF
                     FKVQLYDGTIAYTRDGSFKVDSNRQIVTSNGYLLEPPIVLPENFIMETLSISQDGRVT
                     VKVVGDDDPIEVGQLEIARFVNPAGLQNIGGNLYKTTPASGEEIAGPPGIDGMALTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYEINSKAIQTSDSMLATAIGLKR"
     gene            2755..3294
                     /locus_tag="PLX22_11210"
     CDS             2755..3294
                     /locus_tag="PLX22_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HOT20504.1"
                     /translation="MTYRFFIGVISICFIFVYSQAFADVVLYLKSNVKANDKITIADI
                     ALVDGDEDIKSIKNIELPESCKRDGYFTKDEVFQCIKNYVKGLTIIHGYSVKLITADG
                     SFSNSQDSYNELSPMMKRRTVSKGDFVMVKLIKKSIAIELMGRSMQSGTTGDEITVTL
                     QNGKVVTGTIHDDIIECKL"
     gene            3291..3914
                     /locus_tag="PLX22_11215"
     CDS             3291..3914
                     /locus_tag="PLX22_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOT20505.1"
                     /translation="MKKIFVAIALVIASSVYADTIWKDKNLYTAQDAIKVGDIFTIIV
                     TDISQMRFTLTLANNDAFTVTSAPDGTITPFLPQINASRHATKNNTTNLQHRGNIAIS
                     IAATVQERLANGNFRVNGTKTFSFSGVTTIFNVSGIVNPASIKGGTINSSDIANLVLQ
                     IQGVKESVRITRAPLKEGESASYTLTEQEKQQIIIQYLQRILGELTQ"
BASE COUNT         1271 a          713 c          681 g         1373 t
ORIGIN      
        1 gtaaaatcaa gaggtaatga gattgcatta atgcttaccg atcttgtaat gcccggcatg
       61 aatggagtcg agctctatga agctataaat aaaataaacc cgcatattaa aaatatattc
      121 atgtcagggt ataccgattt agtaatttac gaacatattg taaataagga aagcaattat
      181 ctacagaagc cattttctat gtttgattta gctaaaaagt taaaacatgt tcttacatca
      241 taattactca gatagacatt gctgctataa ttattaaata aggcagcaat gtctataatt
      301 tatttcagaa atttattttc tttcctaaca tatatcttcc cgattgcgct tcatctatag
      361 agccatctgg attataaaat ttccattcac cattcatttt cccatttaaa tattcgccct
      421 cggattttat cttcccattt tgataatatt ctttatatgc accatttaat ttatccatga
      481 gatatatagc ttcaagtttt attgcttttg ttccaggata atattctgtc cattttccat
      541 tttttattgg tgtttcccat ttacctgtcc atgtatagct tatcttttcc ttaggattat
      601 caactgggac tgacgatgat gaaatttcac ctgtttgttt tccatctttt atgatactgt
      661 aaattccttt tggaataccg gtaagttgtc ctgtgccaac gatctgacct ttttcattat
      721 acagcacagc tttttcattt gtattgtcaa tcattccacc ctgaaggggg agttccatta
      781 tctttactcg ctgaacacgt gaaggataca tataaaaatt ccacaatcca atctctttgc
      841 cattgttata tttcccttct ttttgtaata gcccttcttt agaataaaat ttccaatcac
      901 cctccggttt atccatcaca aaacttcctg tcgtagcaac ctgtccatta tcaaagtaaa
      961 atttgaagtt gccgttcttc ttattttctg tatacattcc ttcttcttgt tttttgccat
     1021 tttcatacca tttattacat tgtccgttca attgacctgc tttataccat gctataattt
     1081 ttggtgttcc attttcatag tattcctgag tccttcctgt ttgctcgcca ttggtatatt
     1141 gttcttcttt ccatattttt cctgattgat aataccaaat ttttatccct tcaatggtgc
     1201 cagcttttat ggtaaatccg ctcactgttt tttcttttat tacataggtg gctttccaca
     1261 taatgttgcc gtcaggataa taggctaacc attcccctgt cttttggtcc tctaaaaaat
     1321 taccttctaa actttttgct ccgtttttgt gatagtatgt ccagaaaccc tgctgtttat
     1381 cattgacata ttcgccttca gctaattttt ctccgctacc tttatggtac aatatccatc
     1441 ttcctgtttt aactccatta ataacattcc cctgaccctt caatgatttg tcttcccaat
     1501 agattttaac aagaccattc atagtattat ctgtttttgt atcagtcttt tttgctgaac
     1561 tgctacatga tgttatcaag gcaatcgctg aaagaaatat aatcaaatgt tttgttttca
     1621 taaaaaaatc tccttcgtct attgttatac accttttaat taaaattctc taaatcactt
     1681 ttttcatagt actgacaatt gaaaaaagcg gataaaacaa catctgatgc ctacacaatt
     1741 actaaaaatg taatttctgg atgttattat atagtaagta catagtaaac gttagaaagt
     1801 tacaataatc aaaacaatgt tgtaggcgat agtttcttat tattattttt tgcttttaat
     1861 ctatatttat tatagtaaat cattttgtca atcaattatg tcaagcaaat aaataattat
     1921 gccgaaaaat atatataaga ttatatataa acgaggtgac aatatgatgc gttcattatg
     1981 gactgcagca tctggtatga taggacagca atttaacata gataccattt caaataattt
     2041 atcaaatgta aatactgcag gatttaaaaa gatgcgagcc gaatttgagg atttaatcta
     2101 ccagactgtg cttatggcgg gaacacctgc aaccgaaata agtgaagttc ccacaggaat
     2161 tcaggttggt catggagtaa aagtatcagc cacgcaaaaa ctgtttgagc agggaagttt
     2221 gcaatcaacc ggtaacacaa tggatatagc actggagggt gaaggctttt ttaaagtaca
     2281 attatatgat ggtactatcg cctatacccg tgatggttct tttaaagtag attccaacag
     2341 gcagatagtt acttcaaatg gctatctatt agagcctcca attgtactac ctgaaaattt
     2401 tattatggaa acattgtcta tcagtcagga tggaagggta acggttaagg tagtagggga
     2461 tgatgaccca atagaagttg gacagcttga gattgcacgt tttgtaaatc ctgccggatt
     2521 gcaaaatatt ggtggcaatt tatataaaac aacaccagcc tcaggcgaag agattgcagg
     2581 acctcccgga attgacggaa tggcacttac tcatcagggc tttttagaaa tgtcaaatgt
     2641 taaggttgta gaagagatgg tcaatatgat tgttgcccaa cgtgcatacg agataaactc
     2701 aaaagctata cagacgtctg attcaatgct tgccactgca attggattaa aacgatgacg
     2761 tatcgatttt tcattggtgt cattagcatc tgttttattt ttgtttattc gcaggctttt
     2821 gccgatgttg tgctgtattt aaagtctaat gttaaagcca acgataaaat taccattgct
     2881 gatatagctc tcgtggacgg tgatgaggat atcaagagca ttaaaaatat tgaattgcca
     2941 gaatcgtgta aaagagacgg atattttacc aaagatgaag ttttccagtg tataaaaaat
     3001 tatgtaaaag ggcttactat tatccatggc tactctgtta aactgataac cgcagatggt
     3061 tcattttcaa acagtcaaga ttcatacaat gaactatcgc ccatgatgaa acgtcgtacc
     3121 gtaagcaaag gtgattttgt catggtaaaa cttattaaga aatcaattgc tattgagttg
     3181 atgggcaggt cgatgcaaag tggtacaaca ggcgatgaaa ttacagtgac actacaaaat
     3241 ggcaaggttg tcaccggaac aattcatgac gatattattg agtgtaagct atgaaaaaaa
     3301 tatttgtggc gatagctctg gttattgcat cttcagtata tgctgataca atctggaaag
     3361 ataaaaacct ttatacagca caggatgcta ttaaagtagg tgatatattt actattattg
     3421 ttacggatat atcgcagatg cgatttactt tgacacttgc caataacgat gcttttacgg
     3481 ttacttctgc gcctgatggc accataacac cttttttacc tcagataaat gccagcagac
     3541 atgctaccaa aaataatacc acaaatttgc agcacagggg aaatatagca atctcaatag
     3601 ctgcaacagt acaggaacgt ctggcaaatg gtaattttag agtaaacggc acaaaaacat
     3661 tttcatttag tggcgtaaca accattttta atgtctcagg aatagttaat cctgcttcta
     3721 taaaaggtgg tacaattaat tccagtgaca tcgctaattt agtactgcag atacagggtg
     3781 ttaaagagag tgtacgtata acacgagcac cattgaagga aggcgaatcg gcttcatata
     3841 cattaaccga acaggaaaaa cagcagatta taattcaata cttacaaaga atattgggcg
     3901 aattaacaca ataatagcta tgaaaatggt aaacaaaata attattgcat gtaacctgat
     3961 tttatttttt catgtgagta taaatgcaca ggttgctgta aagattaaag atttaattgc
     4021 aattgatgga ttaaagga
//
LOCUS       DAOSOX010000209         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_22430_length_4030_cov_5.773333, whole genome shotgun sequence.
ACCESSION   DAOSOX010000209 DAOSOX010000000
VERSION     DAOSOX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22430_length_4030_cov_5.773333"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4030
                     /locus_tag="PLX22_11220"
     CDS             <1..>4030
                     /locus_tag="PLX22_11220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOT20506.1"
                     /translation="NDGSSLDDAFRRLVGMSIEKRHTAIADSYAAWDVWCKIKELTSF
                     HKGHFLKHLTNVTAIAHDKKAKRHCGDSLRKFIASHTKGIIECNDTTDIIQQLIAATP
                     DDQFAFIFKTAKDMRNTVAQLLLDKIITIDDISNSVFLDSEEFHIDSDKLKQVKPSHD
                     VLQLYIQSLLLYNVSTTSLISLPLFIAEHKQGSLMHQLQTIMESCICHGSPDIKSNAK
                     YVFLNIGGFNTMKLSKTIFASRHVVFNSIHQYDNLFTRLLSKQADIELTSDIINKLGL
                     EDSNKVYITRDYIIHNTAYNNYEQFLHELLPLIHTSDGKWATTLFTRINTLNKFEYVP
                     CIGKAGNTWKLIFEPLYWKADIDTLFSEASSMHFLAHFVDKSNEGALCYKIMFGIDDD
                     AIFEKKDKLVPVKLKAYRSNLKQTKFNTAVYRKNALDIIHNLAGTDAIKGKIVIICTN
                     DEERDFYYNYCTRYFNTTIFRTDTIPPRFFEKNITYHSNYIVITTIYDVNSIKGGNID
                     YVIVPRLSLGYDAPSINQWRKQHIEELLLNENIEMLQLFVKHFGSAEADKVLGAFIYT
                     YPFLNAMNVLYTMLLNNTIDTVAVTLMDTSSLLLPGNQRMHKKFEEIFKSNDLVEESM
                     VHVENLVKNELVDEIMGKEYLQLNKDLYTMLLHKHWGKDKTFKIFNKDIKAFPDKEYI
                     ISNSGVIRQDDIIHYVMRSFHDPNCKDKDSLVIAATGIGKSLIYQIPAIILSQELEPE
                     LTIVISPLIALMKDQVDGLHKHGVYSVAQFNSTLESESRSVIKNLILEGFINIIYVSP
                     EMIVQSKNFQDMIGRRPPSFLVVDEAHSLSQWGHDFRIDYHIVASTIKQIFKYRDFPV
                     AAFTATAKISKFENLSVVHDIIKIMEMDISRDRALLCTTIRDNLYYHVKTFESGTKLE
                     NYNLDEQKVTFLVNFFKNPARYIANEHKQSILKDTSTDKKYKTIIYCSSVNLTNTLSE
                     YLTSNTNYTFKPFNAKLEQYQKEDIQNRFKAGDGIDGICATTAFGMGVDVPDIRIVIH
                     YDLPQGIEAYYQESGRAGRDGKNSYCLLLYQKKLITKNTSNKLENDYQRVQYLMGRDR
                     IRLYDFSTIYNGIAVFLQQQPDKSKDCYVNGTMLHALFIDQNNDASNKVDSLLFYMSD
                     YYHGDMKGYWCYSKEKASLTYKILKPKIHYSFTSDSTLNKVIWSVHKCLCRYKEQQSP
                     ESKSYVEITHQHLNNMLKKDGNYHFSQNDLYRALSTLITHEVIRIDDVKSYYLNDKKA
                     VGGLTALFDKMIKVCENYSVSTKQDGYGTTCRIDFRKMLTGDHNKTINMNTLELIVSL
                     IVKQYIDGKYNKNTRNKKNKNN"
BASE COUNT         1421 a          663 c          734 g         1212 t
ORIGIN      
        1 tcaatgatgg ctcatcgctg gatgatgcgt tcaggcgttt ggttgggatg tccattgaaa
       61 agcgtcatac tgcaatagcc gattcgtatg ctgcatggga tgtgtggtgc aaaataaaag
      121 agcttacatc atttcataaa gggcattttt taaaacatct taccaatgta acagctatag
      181 cacatgacaa aaaagcaaaa cgtcattgtg gcgatagttt gcgtaaattt attgcatccc
      241 atacaaaagg gattatagaa tgtaatgata ccactgatat tatacaacag cttatagctg
      301 caactcccga cgaccaattt gcttttattt ttaaaacagc aaaggatatg cgaaataccg
      361 ttgcgcaatt gttattggat aaaattatta ccattgatga tataagcaat tcagtatttt
      421 tagattcaga agaattccat attgatagcg ataagcttaa acaggtaaaa ccatcccatg
      481 atgtactaca gctgtatatt caatcactac tactgtacaa tgtaagtact acctcactta
      541 tttcgcttcc tctgtttatt gctgagcata agcagggttc attgatgcat cagctgcaaa
      601 ctattatgga aagctgtata tgccatggta gccctgatat taaaagcaat gcaaaatatg
      661 tttttttaaa tatcggtggt tttaacacca tgaaacttag taaaactata tttgcttcac
      721 ggcatgtagt gtttaacagc atacatcaat acgataacct ttttaccagg ctcctttcaa
      781 aacaggctga tatagaactt accagtgaca ttattaataa gcttgggctt gaggatagca
      841 ataaagtgta tattacacgc gattatatta tacacaatac tgcctataat aattatgaac
      901 aatttttaca tgaactttta ccccttatac ataccagcga tggtaaatgg gcaacaacac
      961 tttttaccag gataaatacg ttaaacaagt ttgagtatgt tccctgtata ggtaaagcgg
     1021 gcaatacatg gaaattgatt tttgaaccgc tgtactggaa ggcagatatt gataccctct
     1081 tttcagaggc ttcatccatg cactttttag cacactttgt agataaatct aatgagggcg
     1141 cattatgcta taaaataatg tttggcatag acgatgatgc catttttgaa aaaaaagata
     1201 aacttgttcc ggtaaagcta aaggcgtacc gatcaaattt aaaacaaaca aagtttaata
     1261 ctgcggtgta tcgtaaaaat gcacttgaca tcatccataa ccttgccggg acagatgcta
     1321 taaaagggaa aattgttatt atatgcacta acgatgaaga gcgtgatttt tactacaact
     1381 attgtacgcg atattttaat accactatct ttagaaccga taccatcccc ccgcgttttt
     1441 ttgaaaagaa tataacttac catagtaatt acattgttat tactaccata tatgatgtta
     1501 acagtataaa gggtggcaat attgattatg ttatagtacc ccgtttgtcg ctggggtatg
     1561 atgctccgtc tattaaccag tggcgaaaac aacatataga ggagttgttg ttaaatgaaa
     1621 atattgaaat gttgcaacta tttgttaagc actttggtag tgcagaagcg gataaggtgc
     1681 tgggagcctt tatatatacc tacccttttt taaatgctat gaatgtacta tataccatgc
     1741 ttttaaataa taccattgat actgttgccg ttacacttat ggataccagc agcttattat
     1801 taccaggaaa ccagcgaatg cataaaaagt ttgaagaaat ttttaaaagc aatgacctgg
     1861 ttgaggaaag catggtgcat gttgaaaacc tggtaaaaaa tgagcttgtt gatgaaatta
     1921 tgggtaaaga gtatttacaa ttaaataaag acctttacac catgttattg cacaaacact
     1981 ggggtaaaga caaaaccttt aagatattta ataaagacat aaaagcattc ccggataaag
     2041 aatatatcat cagcaatagt ggtgttataa ggcaggatga tattatccat tatgttatgc
     2101 gttcattcca tgatcccaat tgtaaggata aagattcgct tgttattgca gctactggca
     2161 ttggaaagtc gctcatatat caaatacctg caattatcct ttcacaggag ttagaaccag
     2221 agctaacaat agtaatatcc ccgcttattg cattgatgaa ggaccaggtt gatggactgc
     2281 acaagcatgg tgtttactcc gttgctcagt ttaattccac tctggaaagt gaatcgcgca
     2341 gtgtaataaa aaaccttatt ttagaaggtt ttataaatat tatatatgta tcgcccgaga
     2401 tgattgttca gtcaaaaaat tttcaggata tgataggacg gcgcccgccc tcatttttgg
     2461 tggtggacga agctcatagc ttatcgcagt ggggacatga tttccgtatt gattatcaca
     2521 tagtagcaag cacaataaag caaattttta aataccgtga ttttcctgtt gcagcgttta
     2581 ctgcaactgc aaagataagc aagtttgaaa acctatcggt tgtacatgat attataaaaa
     2641 tcatggagat ggatatatcc cgcgacagag cgctgctgtg tacaacaata cgtgataacc
     2701 tttattatca tgtaaagaca tttgaatctg gaactaaatt agaaaattat aatcttgatg
     2761 aacaaaaggt tacatttttg gtaaattttt ttaaaaatcc tgcacggtat atagcaaatg
     2821 aacataagca atcaatactt aaagatacat ctactgataa aaaatataaa actatcatct
     2881 actgttcatc ggttaactta accaatacat tatctgaata tttaaccagc aataccaatt
     2941 acacgtttaa gccatttaat gcaaaacttg aacaatacca gaaagaagac atacagaata
     3001 gatttaaagc gggtgatggc attgacggta tatgtgcaac aactgccttt ggtatgggtg
     3061 ttgatgtgcc agatatacgc atagtaatcc attatgattt accccagggt attgaagcct
     3121 actatcagga atcgggaagg gcaggcagag atggtaaaaa ctcatactgc cttttactgt
     3181 atcaaaaaaa attgattacc aaaaatacat ccaataaatt ggaaaatgac tatcaaagag
     3241 ttcaatattt aatgggaagg gataggatac ggttgtatga tttctcaact atttataatg
     3301 gcattgcagt atttttgcaa cagcagcctg ataaaagtaa agactgctat gttaatggaa
     3361 ccatgctcca tgcacttttt attgatcaaa ataacgatgc ttccaataag gtggattcac
     3421 ttctttttta tatgagcgac tattaccatg gtgatatgaa aggatattgg tgctattcaa
     3481 aggaaaaggc atcattaacc tataaaatac ttaagccaaa gattcattat tcctttacca
     3541 gtgacagtac attaaataag gtaatctgga gtgtgcataa atgcctttgc aggtacaaag
     3601 aacaacaaag cccggaaagc aaaagctatg ttgaaattac acatcaacat ttaaataaca
     3661 tgctaaaaaa ggatggcaac tatcatttca gtcaaaatga tttataccgg gcattaagca
     3721 ctttgataac acatgaggtt atacgcatag atgatgttaa aagctactat ttgaacgata
     3781 aaaaagctgt gggcggatta acagcattat ttgataaaat gataaaggta tgcgaaaact
     3841 acagcgtttc aactaaacag gatggttatg gcacaacctg tagaatagat ttcagaaaga
     3901 tgttaactgg tgatcataac aaaactataa atatgaatac gcttgagctt atagtatcat
     3961 taatagtaaa acagtatatt gacggtaaat ataataaaaa tacgagaaat aagaaaaata
     4021 aaaacaatga
//
LOCUS       DAOSOX010000210         3928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_23175_length_3928_cov_7.900077, whole genome shotgun sequence.
ACCESSION   DAOSOX010000210 DAOSOX010000000
VERSION     DAOSOX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23175_length_3928_cov_7.900077"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /locus_tag="PLX22_11225"
     CDS             <1..2373
                     /locus_tag="PLX22_11225"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOT20507.1"
                     /translation="TLYIIGDPKQAIYSFRGADVFAYCKARECAQTKYTLRHSFRSTQ
                     TLTKAINTIFGTMENPFVLDQIDYSGVESSMPPTAFDNEPSLHIWFVDTEAGEKHVTI
                     PKAKTIIAQRIAKEINTLVQEKPIQYSDIAVLVRKHKEARGVQDILQRYGIHSVLYST
                     ESIFESDEAMELLQVMEAIVNPSSAQKIFRALITSLYGYTAQHIVALNDDSKLDSITM
                     RFMDYYNAWKSKGFIQCFQLMMHDDTINRHFNEDIDTVSSRLLHYPDGERVITNLYHC
                     AEILSQAETTKKLDALSLVEWFKKSILIKPDVEENKLRLESDANAVKIVTVHFSKGLE
                     YPVVFCPYLFNVGMLDDIIVFHGGDSNCTTNVCLKLNADKDKTNGDNLYGIDYKHYVN
                     QALKEQLAEDIRVAYVAMTRAKYRCYVGWGNINKTNRSALSYLFHYRFANNTTIDNFL
                     DNNFNNNAASNELKDGLKKLVNLSDGSIALEVFNTVPLDKAMVTQVMDTIVVEPERIQ
                     KDISPGWQLTSYTALSYTKEDEKGDEVYASVPHDEQHEPTIFTFPAGSVTGLCIHSIF
                     EEVDFASGEDAMRTIVAEKLQHYRFDVSWTDTIVTMVRNVLSVELNTAVPLNRITREN
                     RQHEIEFYYPVDRLNRDECVQLFSMNNLFDTHYGSALQRLSFDHIQGFMHGYIDMVFR
                     YNDAYYIVDWKTNQLGYTMDDYHRSKLKSAMIDHFYFLQYHIYAVALHRHLAMLLGKS
                     YNYNKHFGGVFYIFVRGVDTGKGGDYGIFYDKPDCQFIEQLNNYLHAVKV"
     gene            2370..>3928
                     /gene="recD"
                     /locus_tag="PLX22_11230"
     CDS             2370..>3928
                     /gene="recD"
                     /locus_tag="PLX22_11230"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOT20508.1"
                     /translation="MMQQQLYTDSIHYYFADFICNKYNCNDEVVKALLFCVSYMTSYG
                     HSCLSLVTIAHKKLDEIVFEDYTSIPELTFPDVTSLHTVLMECHAVKADDDDAIKPIV
                     YDNGNCYLYRYFNYEEIIAQWFLNSISKSHEIDMQQVNAVLETFEQKGMHFASLQKIA
                     QLAALHHTFCVISGGPGTGKTTTVAGIVYQLLTAHNTLSIALCAPTGKAASRLKESMK
                     QFKDKYCDDAIKDIFPVESSTIHRLLGYKKGALEFYYNEDNRLPYDVVIVDESSMVDI
                     ALLAKLIKALKEGARFIVVGDKDQLSSVEAGAFFGDLCWGVTQFGYTSQWNKTVAAIK
                     DDDAQQLPSHPVRDAIVVLSKAYRFGADSGIAVLAQAVRDADMHKVDNVLKNSYNDVE
                     FIQYSSIDEIHTIIKEYAKQHYESVAIAHNVSDAFSKISHQCIVTPFNQGPWGVSTLN
                     AIIEKQINPLASGWYHGKIIMVTANDYNLQLFNGDMGIFVNNDDGESGVCFPLEDSYR
                     KINPLLLSEYIP"
BASE COUNT         1247 a          675 c          827 g         1179 t
ORIGIN      
        1 acgctgtata tcattggtga tccaaaacag gcaatataca gcttcagagg tgctgatgtc
       61 tttgcctatt gtaaagcgcg tgaatgtgca caaacaaaat atacattacg ccatagcttc
      121 cgttccactc aaacactgac aaaggctatc aatacaattt ttggcaccat ggaaaatccc
      181 tttgtgctgg atcagattga ctatagcggt gtggaatcat ccatgccacc aaccgcattt
      241 gataatgaac cttcattgca tatatggttt gttgatacag aagctggtga aaaacatgta
      301 actataccaa aagcaaaaac gatcattgca cagcgtattg caaaggagat taatactctt
      361 gtacaggaaa aaccaataca gtatagcgat attgctgtgc tggtgaggaa acataaggaa
      421 gctcgtgggg tgcaggatat attacaacga tatgggatac acagtgtatt gtacagcaca
      481 gaatcaatat ttgaatctga tgaagctatg gaacttttgc aggtgatgga ggcaatagta
      541 aatccttcat cggcacaaaa gatattcagg gcactgataa caagcttgta tggatataca
      601 gcacagcata ttgttgcatt aaacgatgat agtaaacttg atagcattac aatgcgcttt
      661 atggattatt ataatgcatg gaagagcaaa ggatttattc agtgctttca gctcatgatg
      721 cacgatgaca caatcaacag gcattttaat gaagatattg atactgtttc atcacgttta
      781 ttgcattatc ctgatggcga gcgtgttata accaatcttt atcactgtgc cgaaattttg
      841 tcacaggctg aaaccacaaa gaagctggat gcactttcgc tggtggaatg gtttaaaaaa
      901 tctatactca taaagcccga tgttgaagaa aacaaattac gattagaatc cgatgccaat
      961 gcggtaaaga tagtaaccgt acattttagc aaaggattgg aatatcctgt ggtgttttgc
     1021 ccataccttt ttaatgtggg catgctggat gatattattg tttttcatgg tggcgatagt
     1081 aactgtacaa caaatgtttg ccttaaattg aatgctgata aggataaaac caatggtgac
     1141 aacctgtatg gaatagatta taaacactat gtcaatcagg cattgaaaga gcagcttgcc
     1201 gaagatatac gcgtggcata cgttgcaatg acgcgggcaa agtaccgttg ttatgttggg
     1261 tggggtaata ttaataaaac taacagatct gcgttaagtt atcttttcca ctatagattt
     1321 gcaaataata ccactattga caattttctt gataacaatt ttaataataa tgcagcttca
     1381 aatgaattaa aagatggatt aaaaaaactt gttaaccttt ctgatggatc cattgcgctt
     1441 gaggttttta acactgtacc tctggataaa gccatggtaa cgcaggttat ggatactatc
     1501 gtggttgagc cagagcgcat acaaaaagat atttcacctg gctggcagtt gaccagctat
     1561 accgcactat cgtacacaaa agaagatgaa aaaggtgatg aggtatatgc ttcagtgccg
     1621 cacgatgaac agcatgaacc aacaatcttt actttcccgg caggcagtgt aacggggctt
     1681 tgtattcaca gtatctttga agaggttgac ttcgcatccg gtgaagatgc aatgcgcacc
     1741 atagttgctg aaaaattgca acactatcgg tttgatgtaa gctggacgga tacaattgta
     1801 actatggtac gcaatgttct ttccgttgag ctcaatactg ctgttccttt aaacaggata
     1861 acacgggaaa atagacaaca cgagattgaa ttttattatc ctgttgacag gcttaatcgt
     1921 gatgaatgtg tgcaattatt ttcaatgaat aacctatttg atacacacta tggcagtgca
     1981 ttgcagcgcc tttcctttga tcatattcag ggatttatgc atggatatat tgatatggta
     2041 ttccggtata atgacgcata ttatattgtt gactggaaaa caaatcagct tggctataca
     2101 atggacgact atcatcgtag caaattaaaa agtgccatga tagatcattt ttatttttta
     2161 cagtatcaca tctatgctgt tgctcttcac agacatctgg caatgctgtt gggaaaaagt
     2221 tataattaca acaaacattt tggcggggta ttctacattt ttgtgcgtgg cgtagatact
     2281 ggtaaaggtg gtgactatgg catcttttat gataagccag attgtcaatt tattgaacag
     2341 ctaaataatt atttacatgc ggtgaaagta tgatgcagca acaattatac actgattcaa
     2401 tccattacta ttttgctgat ttcatctgca ataaatataa ttgtaacgat gaagtagtca
     2461 aggcactatt attttgtgta agctatatga cctcatatgg tcatagctgc ctttcactgg
     2521 taactatcgc ccataaaaaa ctggatgaga tagtttttga agattatacc tccatccccg
     2581 agctgacatt tcctgatgta acatcattgc atactgtact tatggagtgt catgcagtaa
     2641 aagctgacga tgacgatgct ataaagccaa ttgtatatga taatggcaat tgctatttat
     2701 atagatactt taattatgaa gaaattatag cacagtggtt tttgaatagt ataagcaaaa
     2761 gtcatgaaat agatatgcag caggtaaacg ccgtacttga aacatttgaa caaaagggga
     2821 tgcattttgc ttctttacaa aagattgcac agcttgcggc attgcaccat acgttttgtg
     2881 ttatttctgg tggccccggt acaggcaaaa caacaaccgt agcaggcatt gtatatcagc
     2941 ttcttacggc acataataca ttgtccattg cattgtgtgc gccaacgggg aaagctgcat
     3001 cacgccttaa agaatcaatg aaacaattta aggataagta ttgtgatgat gctataaaag
     3061 atatattccc ggttgaatcg tcaacaatac ataggttgct gggctataaa aaaggcgcat
     3121 tagaatttta ttataatgaa gataatcgtc ttccctatga tgttgtgatt gttgatgaat
     3181 catccatggt ggatattgct ttactcgcaa aacttatcaa ggcattaaaa gaaggtgcac
     3241 gtttcattgt agtaggtgat aaggaccagc tttcatcggt tgaggcgggt gcgttctttg
     3301 gcgatttatg ctggggtgtt acccaatttg gctatacatc acagtggaat aaaacagtag
     3361 cagctataaa agatgatgat gcgcaacagt tgccatcaca tcctgtgcgc gatgcaattg
     3421 ttgtgctttc taaagcttat agatttggtg cggatagcgg gatagcagta ttggcgcagg
     3481 ctgttcgcga tgctgatatg cacaaggttg acaatgtttt aaaaaatagc tataacgatg
     3541 tggagtttat acaatacagt tcaattgatg aaatacatac cattatcaaa gagtatgcaa
     3601 agcagcatta tgaatctgtt gccatagcac acaatgtcag tgatgctttt agtaaaatat
     3661 cccatcaatg tatagtaaca ccgtttaatc agggtccgtg gggtgtatca acgctgaacg
     3721 caataattga aaagcaaata aacccccttg cttcagggtg gtatcatggc aagattatta
     3781 tggttaccgc caatgattat aatctacaac tgttcaatgg tgatatggga atatttgtta
     3841 ataatgatga tggtgaaagc ggggtgtgct tcccattaga agatagttac cggaagatta
     3901 atcctttgct gttatccgaa tatatccc
//
LOCUS       DAOSOX010000211         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_23337_length_3908_cov_10.173112, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000211 DAOSOX010000000
VERSION     DAOSOX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23337_length_3908_cov_10.173112"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLX22_11235"
     CDS             <1..1059
                     /locus_tag="PLX22_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20509.1"
                     /translation="LCKATDILIELIANHLGLPPHYYNAPDAEIKSRIQFAEFKSYTL
                     PSIIGNIHKDENDTRSIESELQTLQADSSDATINEKFEDEKLNPHNHKVESKKIFNRN
                     DQNNELTKQANVFFTVKGSKPWNTRDPYILPVDENVLLVEEEDLEYMVYYSRTSMNEE
                     QIKAELRRALLKLTIDRQKYDIVEEYIEYINKKSISVLQEMLEYFHIDDTDQWLFYYH
                     LGPQTICRIVLADMQLTGEGYCFSSRDGKKIRKLLPYEFIKKMSLEWCENNINNRDIP
                     FDSVNALDLIRRQVSKKYYSEVDEFVTKIDSYLGTLDSEILKKLDRRLFIKQKALQIY
                     NPKQILVYNRFIDKTIFK"
     gene            1070..1771
                     /locus_tag="PLX22_11240"
     CDS             1070..1771
                     /locus_tag="PLX22_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT20510.1"
                     /translation="MFDIKKYHGDEKFLKILDKNAIAIDIYTLKAAILGVQASGGDFS
                     MKMLAKDFFSDEALDNKALADQLESLWFYLLRLQRFDDGELEQPCYEDKTREMYLSYV
                     KSSILRAETFLKYLAMGSIDEMRKNKKVDTIYSLFIGNVNLLKSLQTSMEEKWTDEDF
                     TGDTTFLQKLTGFIRVMWDTKIALKEHAKIFKLQKMNNKNIIHVLEKQTGAQIGRNEQ
                     CPCGSGKKYKNCCGS"
     gene            complement(1798..3882)
                     /gene="recG"
                     /locus_tag="PLX22_11245"
     CDS             complement(1798..3882)
                     /gene="recG"
                     /locus_tag="PLX22_11245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT20511.1"
                     /translation="MLDKSIQYIKGIGPKRAALLYNELGLETVEDLLYFLPRKYIDRS
                     NTKSINNCFVNDFVTVMGIVKQVKISGHKKKFLEIVIDDGTDTLSGVFFGGIQYFIKL
                     FEKGDTVLFSGRVDYYTNKQIIHPEYDFIGDSSDTLHTGRIVPIYPSTENLKTNGFNS
                     RFFRKIIYTVLSEANQYITEPFDEDMLNRLQCMPLSQAIECIHFPSSMDEAYNARKRL
                     AFNELFFLQYYLHLSRQYIRQTNKQNKPMINTATYHAFIKNLPFQLTGDQLQAIEEII
                     NDLNADFPMNRMLQGDVGSGKTVVSLIASIAVVSRGQQVAFMAPTEILAQQHYATFKR
                     LLPNTISVAVLTGSTSQAERKSILNGLNNSSIDVIIGTHALIQETVEFANLGFIIIDE
                     QHRFGVNQRAQLRTKGAIPDLLVMTATPIPRSLSLTLYGDLDISIIKEKPANRIPITT
                     LAFPVSKLEAVYRSLEKYMDEGRQVYYVLPLIDDSEKLDLKSATATFKQLQKRFPNKS
                     IALLHGKLPNNDKEKIMSLFNNHALDMLVSTTVIEVGIDVANASIIVIEHAERFGLSQ
                     LHQLRGRVGRGTYKSFCILIYPDSITPESMQRINILTSTDDGFIIAEEDLKIRGAGEF
                     IGTRQHGKGIAFEFADPINDFELISIAREEAKKKVESLQDIPALFENSKRLHTINILE
                     GIRKKHILAMLS"
BASE COUNT         1280 a          636 c          734 g         1258 t
ORIGIN      
        1 ttatgcaagg caaccgatat tttaattgag ttaattgcca atcatttagg attgcctccc
       61 cattattata atgcaccaga tgctgagatt aaatccagaa ttcaatttgc tgaatttaaa
      121 tcctatacat tgccatcaat aattggtaat attcacaaag atgaaaatga taccaggtca
      181 attgaatcag agctgcaaac attgcaggca gattcatctg atgctacaat aaatgaaaag
      241 tttgaagacg aaaaactgaa tccccataat cataaagtag aatcaaaaaa aatttttaat
      301 cgcaatgatc aaaataatga attaaccaaa caagctaacg ttttttttac agttaaaggc
      361 agcaaaccat ggaatacgcg cgatccctac attcttccag ttgatgagaa tgtgttgctt
      421 gttgaagagg aagatcttga atatatggtt tattattcca gaacatcaat gaatgaagag
      481 caaattaaag cagaattacg aagggcttta ttgaaattaa ctattgatag acaaaaatat
      541 gatattgttg aagaatatat agaatacatc aataagaaga gtatttcggt attacaggaa
      601 atgcttgaat attttcatat cgacgatacc gatcaatggc ttttttatta ccatttgggc
      661 cctcaaacta tatgtcgcat tgttttagca gatatgcagc ttactggtga gggttactgt
      721 tttagttcac gtgatggtaa aaagataagg aaattattac cttatgaatt tattaagaag
      781 atgtctctgg aatggtgcga aaataatatt aataacaggg atataccgtt tgatagtgtc
      841 aatgcattag atttaatacg aaggcaggtt tctaaaaaat attattcaga agttgatgaa
      901 tttgttacaa aaattgattc ttatctgggg acgctggatt ctgaaatttt gaaaaaactt
      961 gataggcgat tatttataaa acaaaaagct ttacagatat ataacccaaa gcaaatactg
     1021 gtatataatc gatttataga taaaacaatt tttaaataat agggagccca tgtttgatat
     1081 aaaaaaatat cacggtgatg aaaaattttt aaaaatctta gataagaatg ctattgcaat
     1141 agatatttat acccttaaag ccgcaatcct tggtgttcag gcttcagggg gggatttttc
     1201 aatgaaaatg cttgcaaaag atttttttag tgatgaagca ctggataata aggctctggc
     1261 tgatcaactg gaatcgttat ggttttatct tctgcggttg cagcgttttg atgatggtga
     1321 actggaacaa ccctgctatg aggataaaac acgagagatg tatctatcct atgtaaaatc
     1381 atcaatactt cgtgcagaaa cctttttgaa atatcttgca atgggaagta ttgatgaaat
     1441 gagaaaaaac aagaaggttg atacaattta ctcattgttt ataggtaatg ttaatttatt
     1501 aaagtcatta caaacaagta tggaagaaaa atggactgat gaggatttta ccggggatac
     1561 aacatttttg caaaaattaa ccggatttat acgtgtaatg tgggatacca aaattgcgtt
     1621 gaaggaacat gcaaaaattt ttaaattgca aaagatgaat aataaaaata tcattcatgt
     1681 actggaaaag cagactggtg cacagattgg gcgcaatgaa caatgcccct gcggcagtgg
     1741 gaagaaatac aaaaattgtt gtggctcata gatataaccc ggtagtttgc cgttatttca
     1801 tgagagcatt gccagtatat gtttttttcg tattccttcc aggatgttta ttgtatgcag
     1861 acgtttgctg ttttcaaata gtgcaggtat gtcttgtaat gattctactt tcttttttgc
     1921 ttcttcacgt gctatgctta taagctcaaa atcgttgata gggtcggcaa attcaaaagc
     1981 tatacctttt ccatgctggc gtgttccaat aaattcgccg gcgccacgta ttttcagatc
     2041 ttcttcggca atgataaagc catcatcggt agaagttaaa atatttatgc gttgcatact
     2101 ttcgggtgta atagagtcag gatatatcag aatacaaaat gatttgtatg taccacgccc
     2161 aacacgtcca cggagctgat gaagctgaga taatccaaaa cgctctgcat gctcaataac
     2221 aataatagaa gcattggcaa catcaatccc aacttcaata acggttgtac tgacaagcat
     2281 atccagtgca tgattattaa agaggctcat aatcttttct ttatcgttgt ttggaagttt
     2341 tccatgcaga agtgcaatgg atttattggg aaatcgtttc tgtaactgtt taaaagtagc
     2401 ggttgctgat tttagatcta atttttcaga atcgtcaata agaggcagta catagtatac
     2461 ctgtctgccc tcatccatat acttttcgag tgagcggtat actgcttcca gttttgatac
     2521 tggaaatgct aacgttgtta tgggaattct attagctggc ttttctttta ttattgaaat
     2581 atcaagatcc ccgtataatg ttagcgatag tgaccgggga atgggagttg ctgtcataac
     2641 cagtaaatca gggatggcac cttttgttct tagctgggca cgctgattga caccaaacct
     2701 atgctgttca tcaataatga taaaacctaa attggcaaat tctactgttt cctgaataag
     2761 agcatgtgtt ccaattatta catcgatgct gctgttgttt aaaccattaa gaatagattt
     2821 acgctcagcc tgtgatgttg aaccagtaag tacagcaaca gagatggtat tgggtagcag
     2881 ccttttaaag gttgcataat gttgctgtgc caatatctcg gttggtgcca taaacgctac
     2941 ctgctgacct cggcttacca ctgctatact tgcaataagg gaaacaacgg ttttaccgga
     3001 accaacatcg ccctgtaaca tgcggttcat tggaaaatct gcatttaaat cattaataat
     3061 ttcttctatt gcctgtagct ggtcaccggt taattgaaat ggaagatttt ttataaatgc
     3121 atgatatgtt gcagtattta tcattggttt attttgttta tttgtctgtc ggatatactg
     3181 tcgtgaaaga tgcagatagt attgcaaaaa gaaaagttcg ttgaacgcaa gcctcttccg
     3241 tgcattgtaa gcttcatcca tagatgatgg aaaatgaata cattcaattg cctgtgataa
     3301 cggcatgcat tgtaaacggt taagcatgtc ctcatcaaaa ggttcagtaa tatattggtt
     3361 tgcttctgat aatactgtgt aaataatctt tctgaaaaac cgtgaattaa aaccatttgt
     3421 ttttaaattt tcggttgagg gatatatggg gactattctg cctgtatgca atgtatcaga
     3481 actatctcca ataaaatcgt attcgggatg tattatctgc ttattggtgt agtaatcaac
     3541 tcgtcctgaa aataaaaccg tatcaccttt ttcaaaaagt ttgataaaat actgaatgcc
     3601 tccaaaaaat acccctgaaa gagtatcagt accatcatca ataactatct ccaaaaattt
     3661 ttttttatgg cctgatatct ttacctgttt tacaatgccc attacagtaa caaaatcatt
     3721 gacaaaacaa ttgttaatgc tttttgtatt ggaacggtca atatatttcc ttggcaaaaa
     3781 atacagtaaa tcttcaacgg tttcaagccc taattcatta tataacagtg ctgcccgctt
     3841 tgggccaatg cccttgatat attgaatgct tttatcaagc acggttactc ctcttcagca
     3901 atatacca
//
LOCUS       DAOSOX010000212         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_23343_length_3907_cov_8.808411, whole genome shotgun sequence.
ACCESSION   DAOSOX010000212 DAOSOX010000000
VERSION     DAOSOX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23343_length_3907_cov_8.808411"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1081)
                     /gene="fmt"
                     /locus_tag="PLX22_11250"
     CDS             complement(149..1081)
                     /gene="fmt"
                     /locus_tag="PLX22_11250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT20512.1"
                     /translation="MKIGFFGTPDIASYCLDYLLKKYDIAFVITTPDKPKGRSKDPVP
                     SPVKEKAAALGIPCFQPLNLNDTAIIQTLKNFHCDIFVVVAYGKLIPGDVFLLPPLGT
                     INLHPSLLPKYRGAAPVQWALYNGEHEIGITVQLINEQLDAGDIVMQKTIPVDLHDTS
                     ADVYERIKPIGAEMLAESVDCLFKGTIAPKPQDHALATYCGKITTETAHINWELSSVQ
                     IHNMVRAFNPKPIAWTMFREKLIKIWKTDLPDNSSEKMIGQPGTLVPASKRLFVCTGR
                     GMIEVLLLQPETKKPMDAIAFLNGYRLQEGERFI"
     gene            complement(1081..1599)
                     /gene="def"
                     /locus_tag="PLX22_11255"
     CDS             complement(1081..1599)
                     /gene="def"
                     /locus_tag="PLX22_11255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT20513.1"
                     /translation="MITDTQHTLVYYGNNILRTTAQEVVQISQMVRDLVDEMFAIMHR
                     EQGIGLAAVQIGVPLQVIVIDIRSNKGPMMALINPEITAFSEEKDVYEEGCLSLPGVM
                     ADVIRPLSIQVEGIAINEKPVKFEASGLLARVLQHEIDHCNGKLFIDYLEDYKRKELN
                     SQLKKIKKLNRE"
     gene            1981..3471
                     /locus_tag="PLX22_11260"
     CDS             1981..3471
                     /locus_tag="PLX22_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOT20514.1"
                     /translation="MAFNEYEKYDATGLAELIQKKKVSVYEVINEAIQRMQTINPVIN
                     AVITPMYENIDARIKELPKGRFYGVPFFIKDLLTTYKGVRMASGCRAYKNYIPNFHSE
                     VAKRYINAGVVILGKTNTPEFGLMAYTEPEAFGPTLNPWNIALTPGGSSGGSAAAVAS
                     GMAPFAGGGDGGGSIRIPSAYCGLFGLKPSRGRVPAGPEYGQIWQGAAVEHVLTRSVR
                     DSAAMLDILQGADIGAPYIIEPPEMPYTKLMQKSPGKLTIAISTESPIGTQVHPECKK
                     AAQEAAKLCERLGHHVEYDTPDIDGIALAKSYLMLYFGEVAADIATIGLTLGRKTKQS
                     DVEITTWTLGMLGKAYGAGDFVLALREWNKASRILGQFFTKYDIYLTPTTAQPPAPIG
                     SLKPKPIELFASKIMNTLGLGKLLKASGIVDKLAVESLSQVPFTQLANLTGVPAMSVP
                     LHWGSDGLPYGVQFIASSGKEGLLLQLAAQLEKAKPWFNKRPDLTI"
     gene            3712..>3907
                     /locus_tag="PLX22_11265"
     CDS             3712..>3907
                     /locus_tag="PLX22_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20515.1"
                     /translation="MKKLYKALIETIYFIYRPVFYVLVVIDTTILGILTIALSFFDPT
                     GNTVHYIGVFWSQLNLFLSGV"
BASE COUNT         1178 a          780 c          747 g         1202 t
ORIGIN      
        1 tggcaatttt tttcttccac catgaggaat gtcgcaactc ccttgctttt tgcttctcac
       61 gcttaatatg caactcatta tcagaaaaat ttataccaga tacagtggca ttgtcatcaa
      121 aaaatttcct gtatctatct cccatatatt atataaaccg ttccccttcc tgtaatcgat
      181 aaccatttaa aaatgcaatt gcatccatag gcttctttgt ttcaggttgc agtaaaagca
      241 cctcaatcat acccctgccg gtacaaacaa acaggcgttt tgaagcaggt actaatgtcc
      301 ccggttgccc aatcattttt tccgatgaat tgtcaggtaa atctgttttc catattttta
      361 taagcttctc cctgaacatc gtccacgcta tgggttttgg attaaatgcc ctgaccatat
      421 tatggatttg taccgaactc agttcccagt taatatgagc tgtttcagtt gtaattttac
      481 cacaataggt agcaagcgca tggtcctgtg gttttggggc gatagttcct ttaaaaagac
      541 aatcaacaga ttctgcaagc atctctgcac caattggttt aatcctttca taaacatctg
      601 ctgaggtatc atgcaaatca acagggatag ttttttgcat cacgatatca cctgcgtcaa
      661 gctgttcatt aattaattgt acagtaatac caatttcatg ttcaccatta tacaatgccc
      721 actgcacagg agcagcacct cgatattttg gcagaagcga tggatgaaga ttgatagtgc
      781 ctaatggagg caacaaaaaa acatctccgg gaataagttt gccatacgca acaacaacaa
      841 agatgtcaca atgaaaattt tttagtgttt gtattattgc agtatcattt aaattcaacg
      901 gttgaaaaca cggaatgccc aatgcagcag ccttttcctt taccggggaa gggacaggat
      961 ctttactgcg cccttttggt ttatcagggg tggtgatcac aaaagcaata tcatactttt
     1021 taagtaaata atccagacaa tatgaagcaa tgtccggtgt accaaaaaaa cctattttca
     1081 ttattccctg ttaagctttt ttatcttttt aagctgcgaa tttaattctt tccgcttgta
     1141 atcttccaga tagtcaataa atagttttcc attacaatga tcaatctcat gctgtaaaac
     1201 ccgtgcaagt aaccctgatg cttcaaactt taccggcttt tcattaatag ctattccttc
     1261 aacctgaata gatagcggac gaataacgtc tgccattact cctggcaacg ataaacaacc
     1321 ctcctcatag acatcttttt cttcagaaaa tgctgtaatc tcaggattta tcagtgccat
     1381 catgggtcct ttattagagc gtatatcaat gacaatgacc tgcaatggca caccaatctg
     1441 cacagcagca agcccaatac cctgctctct atgcataatg gcaaacatct catcaactaa
     1501 atcacgtacc atttgtgata tctggacaac ctcctgcgct gttgtccgca atatgttatt
     1561 gccataatag actaatgtat gctgtgtatc ggttatcata actatctcac aatatcattt
     1621 tatatataat taattataat taatatatca cacagagcat agcatgtcaa gatatgaatt
     1681 ttcgaaaact tatttttact gtaatttatc acaatactaa ttgacaattt gttatatttt
     1741 tcatacactt tgatacgatt ttagtccagc agtttatcct gtgttaccaa ataatttctg
     1801 ggtgcagaat acaactaaaa cgatagttac catgcaatta cttaaaacag gataaaatgc
     1861 aacactgcta tcattatgaa cgatagcacc tgtactggat gtagttgagc aacttatgtt
     1921 gttgttgctc tgatatatta ttctgaatga cacactatat tttatttctg gaggaatacc
     1981 atggctttta acgaatacga aaaatatgat gcaaccgggc ttgctgagct tatacaaaaa
     2041 aagaaagtat cggtatatga ggtcatcaac gaagcaatac agcgtatgca aacaataaat
     2101 cctgttatca atgctgttat tactcccatg tatgaaaata ttgatgcaag gataaaagag
     2161 ctgccaaaag ggcgttttta tggcgttcca tttttcatca aggatttatt aacaacctat
     2221 aaaggcgtga ggatggcaag cggctgcagg gcatataaaa attatatccc caattttcac
     2281 agtgaggttg ccaaacggta catcaatgca ggtgttgtaa tattaggcaa aaccaatact
     2341 ccagaatttg gattaatggc atatacggaa ccagaggcat ttggtccaac ccttaaccca
     2401 tggaacattg ctctgacccc tggcggttcc agcggtggtt ctgcagccgc tgttgcttcg
     2461 ggcatggctc cttttgcagg tggcggtgat ggaggaggtt ctatacggat accgtcagca
     2521 tattgtggat tatttgggct taaaccatcg cgcgggcgag ttcctgccgg tccggaatat
     2581 gggcaaatat ggcagggtgc agcggtagaa catgttttaa cccgctcagt gcgagatagc
     2641 gcagccatgc tggatatact gcagggtgct gatattggcg caccatacat cattgaacca
     2701 ccagagatgc cttacactaa attaatgcaa aagtcaccag gaaaacttac cattgccatc
     2761 tctactgaat cgcccattgg tacccaggta catcctgaat gcaaaaaggc agcacaggaa
     2821 gctgcaaaac tgtgtgagcg gttaggacat catgttgaat atgatacacc agatattgac
     2881 ggtatagcgc ttgccaaaag ctatctgatg ctttattttg gtgaggttgc tgctgatatt
     2941 gcaaccattg ggctaacgtt gggaagaaaa acaaaacaaa gcgatgttga gataacaaca
     3001 tggacattag ggatgctggg aaaggcttat ggtgctggag attttgttct tgcattgcga
     3061 gaatggaata aagcctcacg catactggga cagtttttta ctaaatacga tatatacctt
     3121 actccaacaa cagctcagcc gccagcgccc attggttcac tgaaaccaaa acctattgag
     3181 ctttttgcat caaagatcat gaatacattg ggtttgggaa aattgctgaa agcatccggg
     3241 attgttgaca aattagcagt agaaagttta tcacaggtgc cgtttaccca attagctaat
     3301 ttaaccggtg tccctgcaat gtccgttccc ctgcactggg gtagtgatgg tttaccgtat
     3361 ggtgttcagt ttattgcttc atctggtaag gaaggattgc tattacagct tgcagctcaa
     3421 ctggaaaaag caaaaccatg gtttaacaag cgaccagatt tgactatatg actaatataa
     3481 tgagtaaatt gttatagtat aaagttgttg atacagctgt atgtaactgc tgatattcct
     3541 caagtttttt aaaaggtgta tcagatagta tgtatatatt gaaaactaaa ttttttatta
     3601 tgctgggcac aaaaaatggt aaaaccactt tgttgatcgc tgttacactg acaatagtgt
     3661 tgatcaaatg taaaaggtgg ttttcaatgt gcctccagga tggaggtatt catgaaaaaa
     3721 ttatataaag cattgattga aacaatctat tttatataca ggccagtttt ttatgttctg
     3781 gttgttattg atacaaccat ccttggtatt ttgaccattg cactttcttt ctttgatcct
     3841 actggcaaca ccgttcatta cataggtgta ttctggtcac agttaaatct atttctttcc
     3901 ggtgtca
//
LOCUS       DAOSOX010000213         3829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_23913_length_3829_cov_8.301802, whole genome shotgun sequence.
ACCESSION   DAOSOX010000213 DAOSOX010000000
VERSION     DAOSOX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23913_length_3829_cov_8.301802"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3404)
                     /gene="dnaE"
                     /locus_tag="PLX22_11270"
     CDS             complement(<1..3404)
                     /gene="dnaE"
                     /locus_tag="PLX22_11270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT20516.1"
                     /translation="MDFVHLHNHSEYSILDGAIRIQQLIQFAVDNAMPAIALTDHGNM
                     FGTIKFYEEAHKAGIKPIIGQEFYIAPESRFHKQSTRGSNDENAYHLILLAQNYEGYK
                     NLVKLSSLGYLEGFYYKPRIDWELLTQYSTGLIASTACIAGEIPSLILDGKVSQAYKR
                     ASEFADLFGKDKFFLELQDHNLKEQKKVNEELIKMAAQLNVGLIATNDAHYLNKDDAY
                     YHDVLLCIQTGKSLNDQSRMRFPNSEFYLKTPGQMETIFEDYPDALYNTVKIAEMIDC
                     QIPLGNAILPNFEVPEGYTKDSYLTELVNTGAQKRFGAIVPDYVKHRIEYELSVIQRM
                     QFSGYFLVVWDFIQFAKKHGIPVGPGRGSAAGSMVSYCLEITELDPMKYNLLFERFLN
                     PERNEMPDMDIDFCALRRDEVIDYVKQKYGEDHVSQIITFNKMKSKGAIRDVARALEI
                     PLQQVNALTKLIRHEDLEEALQASDELKQLCKTNDTGKQLITIAKKVEGLTRSAGKHA
                     AGVVISREPLMEHVPLYRDPKDGSISSQYEKVSLEKAGLVKMDFLGLANLTIIDTCIK
                     LIKKHKNIAIDITAIPLNDAKTYKLLQKADTMGVFQLESSGMQNILLKLGPTNFDDII
                     AIVALYRPGPLDSGMVEEFIERKRNPQKVEYLHPSLEPILKDTMGVIVYQEQVMLISQ
                     VMGGFTLPDADKLRKAMSKKKMDIIDAMEEKFVQGAKQKKIDESVARTMYDMMRTFGR
                     YGFNKSHSAAYALVSYQTAYLKAHYPLEFMTALLTAQVDNIDNIAKYYTDCKAKGIAV
                     LPPDINHSYRDFSIEGKAIRYGLISIKGVGDKAIESIIEAREKVDSFHDLTQFFTHID
                     LMTVNKGVIESLIKAGAFDPVYPNRGELFASVDSMLEKARNIQQDIATGQGDLFGASD
                     KQFSRINIEPAPAADWTENVKLQYEKEVLGIYVSAHPLSRYAKDIEKFACTPIKDLEN
                     NVNGSPITLVGILNNVQIKQSQKGNKFAQAAIEDLTGSLQVLLMPQTLSEYETLITSQ
                     EPVVITGTVEVENEKPSRMIAQKIFQFTQFKLTQISELHIIINPIGTNTELLEKIKKL
                     LQRYQGNKPVYFHVRLPNGNENIIKANPLYHIKPDKKLMDGLTAIVG"
BASE COUNT         1066 a          771 c          726 g         1266 t
ORIGIN      
        1 ccaacaatgg cagttaatcc atccataagc tttttatctg gttttatgtg atacaaaggg
       61 ttggctttaa taatattttc atttccgttt ggaagacgta catgaaaata taccggctta
      121 tttccctggt agcgctgtaa taattttttt attttttcaa gtaattctgt atttgttcct
      181 attggattaa taatgatatg caattcgctt atctgggtaa gtttaaattg agtaaattga
      241 aaaattttct gtgcaatcat cctggagggc ttttcatttt ctacttcaac tgtaccggtg
      301 atgacaacag gttcctgaga tgttattaac gtttcatatt cggataatgt ttgtggcata
      361 aggagcactt gcaaactgcc tgttaaatct tcaattgcag cctgagcaaa cttattcccc
      421 ttctggcttt gctttatctg cacgttgttt aatataccta ccagtgtaat aggactgcca
      481 ttaacattat tttccagatc ttttatggga gtgcatgcaa atttttctat atcttttgca
      541 taacggctca aggggtgagc gctaacataa attcccagca cttccttttc atattgcagc
      601 ttaacatttt cagtccaatc agcagcaggg gcaggttcaa tattaatacg tgaaaattgc
      661 ttatcagagg ctccaaataa atcaccctga ccggttgcta tatcctgctg aatgttcctc
      721 gccttttcaa gcatgctatc aactgatgca aaaagttccc ctctgtttgg atatacagga
      781 tcaaaagctc ctgccttaat gagcgattct ataacacctt tgttaacggt cataagatct
      841 atgtgagtaa aaaactgcgt taaatcatga aagctgtcca ctttctccct tgcttcaata
      901 attgattcaa tagctttgtc gccaacacct ttaatagaga taagtccata ccgtatcgct
      961 ttaccctcaa ttgagaaatc tctatagcta tgatttatat cgggtggtaa cactgctatc
     1021 cctttagctt tgcaatccgt gtaatacttt gcaatgttat cgatgttgtc cacctgagct
     1081 gttaaaaggg ctgtcataaa ttctaatgga tagtgtgctt taagatatgc ggtttgatat
     1141 gaaactaaag cataggctgc agaatgtgat ttattaaatc catagcggcc aaaggttcgc
     1201 atcatatcgt acatggtgcg ggcaactgat tcatcaatct ttttttgctt tgcaccctga
     1261 acaaattttt cctccattgc atcaatgata tccatcttct ttttgctcat ggcttttcta
     1321 agtttatcag catcgggtaa tgtaaagcct cccattacct gtgatatgag catgacctgc
     1381 tcctgataaa caataacacc catcgtatcc tttaaaattg gttccagtga aggatgcaaa
     1441 tattctactt tttggggatt acgcttgcgt tcaataaact cttcaaccat gcccgaatca
     1501 agaggtcccg ggcgatagag tgcaacaatt gcaatgatgt catcaaagtt agtaggtccc
     1561 aatttaagga gaatattttg catgccgcta ctttcaagct ggaatacacc cattgtatcg
     1621 gctttctgta aaagcttata tgtttttgca tcattaagag ggatagctgt tatatcaata
     1681 gcaatgtttt tatgtttttt aataagcttt atacaggtat ctatgatggt aaggttggct
     1741 aatcctaaaa aatccatctt caccagtcca gccttttcca aacttacctt ttcgtattgt
     1801 gatgaaatgc ttccatcttt gggatctctg tataatggca cgtgctccat caacggctcc
     1861 cgtgaaatga caacacctgc tgcatgtttt cctgcagagc gtgtaagccc ctctaccttc
     1921 tttgcaatag tgatgagctg cttgccggta tcatttgttt tgcataattg tttcaactca
     1981 tccgaagcct gcaatgcctc ttcaagatcc tcatggcgta tcaattttgt aagggcatta
     2041 acctgctgta atggtatctc aagggcacga gcaacatcgc ggatagcacc ttttgatttc
     2101 atcttgttaa aagtaataat ctggcttaca tggtcttcgc catatttttg ttttacatag
     2161 tctatgactt cgtcacggcg cagagcacaa aagtcaatat ccatatcagg catttcattg
     2221 cgttcgggat ttaaaaatcg ttcaaagagc aggttgtatt tcataggatc aagctcggta
     2281 atctccagac agtatgaaac cattgaacct gcggcagaac ctcgccccgg tccaaccggt
     2341 atgccatgct tctttgcaaa ttgtataaaa tcccacacaa caaggaagta tccggaaaac
     2401 tgcattcgtt gtataaccga tagttcatat tcaatacggt gcttcacata atcaggaact
     2461 atcgccccaa accttttttg tgcccctgta ttaacaagct ctgtaagata actatctttg
     2521 gtgtaacctt ctggaacctc aaaatttggc aggatagcat tccccaacgg tatctggcag
     2581 tcgatcatct cggcaatttt gacagtgttg tacagtgcat cagggtaatc ctcaaaaatg
     2641 gtttccattt gtccgggagt ttttagataa aattcagaat tggggaaacg catccggctt
     2701 tggtcgttca ggcttttccc ggtttgtatg cagagcaaaa cgtcatggta gtatgcatcg
     2761 tctttattga gatagtgagc atcattggtg gctataagcc caacattgag ttgagcagcc
     2821 attttaataa gttcttcgtt aacttttttt tgttctttca gattgtggtc ctgcagttca
     2881 aggaaaaatt tgtctttgcc aaatagatcg gcaaattcag atgcacgttt atatgcctgg
     2941 cttaccttgc catcaagaat gagtgaaggt atttcaccgg ctatgcaggc agtgcttgca
     3001 atgagccctg tgctatactg cgttagcagt tcccaatcaa tgcgcggttt gtagtaaaat
     3061 ccttccaggt accccagtga cgatagttta acaagatttt tgtagccttc ataattttga
     3121 gcaagcaaga ttaaatgata tgcattttca tcattgctgc cccgtgtgct ctgtttatga
     3181 aaccgtgatt ctggtgctat ataaaactcc tgcccgatga taggttttat cccggcttta
     3241 tgtgcttctt cgtaaaattt tatggttcca aacatattgc catgatcggt aagcgctatt
     3301 gcaggcatgg cattatccac ggcaaattgt atcagttgct gtatgcggat tgcaccatcc
     3361 agtatggaat attcagaatg gttgtgaagg tgtacaaaat ccatagcttt ctctcattgt
     3421 gttaacatac taaaattttt cagcttgcga aaatttcaat ataccatgca tggcatggca
     3481 taatatttgt atgattgtaa tttccatatg tgccagggtg cgttttgtac agcagggatt
     3541 caacagtaac tatcgcccaa caaatacgac ataattaaaa taccactata aacactatag
     3601 tggcaggtat ttgcaatttt caagctaaat tttaaaaaat tgtaaattgt atagcgacaa
     3661 taattaaaaa aacaggttgc cagaattaaa aaagcaaatt ataatggcaa tgaaattacg
     3721 gtgtgtgcct gcaatgtacg tgtttatttt agcacagata gtgcattccc aatttatttt
     3781 tcaggagtaa gctgatgatt gatacaaagc tgcttgacat tttagcatg
//
LOCUS       DAOSOX010000214         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24020_length_3817_cov_10.299841, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000214 DAOSOX010000000
VERSION     DAOSOX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24020_length_3817_cov_10.299841"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLX22_11275"
     CDS             <1..687
                     /locus_tag="PLX22_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20517.1"
                     /translation="VKSFSEYQSMLDSNFFKIAMADSSGNSVVSSEINELVVLGTISG
                     PATIARALIKRQSESSAQIYKIGMDVYGFKLIKIDNAKVYLKKDGKIEIIDMFAKEKP
                     QEALSSQQTMQQGTTSANRVAKSISKAEIQQKVLNNLDNALNGVRAGPYRVGGKIEGF
                     KLFSVAPFNILYSMGARSGDIVKRINGQPIDSTEKLMKMWETLKTDTRFVIELERSGK
                     MITYDLNVSE"
     gene            775..>1274
                     /locus_tag="PLX22_11280"
     CDS             775..>1274
                     /locus_tag="PLX22_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20518.1"
                     /translation="MIFFTIIYAQQENITPDKSVDSQIQAEITPKAVEANPQSQETVS
                     QNEQKDVTQKPEIKIEKFKGKKKDEEKYFTLNFKDVELAEFLSVMSQLIGKNIALDER
                     VRGKITISSVKKIPVRDAYDIMKAILELKGFAVVESENLIKIIPIKDAIKKNVEVIID
                     TTKKQV"
     assembly_gap    1275..1374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1375..2460
                     /locus_tag="PLX22_11285"
     CDS             <1375..2460
                     /locus_tag="PLX22_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOT20519.1"
                     /translation="ADAEKLAAVLSRIPFAEYAAISVGETQTPQTVEAIKKAQQAVQA
                     KSSITDKQKLSIIANKETNSLIITAKPEEFKMIKNIIDQLDIVREQVLIEALIIEVVA
                     DSGWGFGIDWMIGDQSGTHLYGGSSIQGTPPSYTLPSGMTGKEVAVPLSTGFQIGYLS
                     DKSMLGFALLNATGAEKHYNILSTPQILTTDNQEAEINVGEEIPVPTNNRISDTGVQF
                     YTYEYKSVGVKLKITPHITSENSITLDLYQEVNSVLGQTTVLESGSIIPPTLGKRDIK
                     TKINVADSKTIVVGGLITNNKTEEETKVPILGDIPLLGWFFKRKVVEYKKTNLLVFIT
                     PHIVTKQAKMDALSEQKREEQRRLQLE"
     assembly_gap    2461..2560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2571..>3817
                     /locus_tag="PLX22_11290"
     CDS             2571..>3817
                     /locus_tag="PLX22_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT20520.1"
                     /translation="MIGIKTKKLGQILIDKRLISQEDLQDALLSQQNTPLKIGQILIK
                     KGLIAEDEVLKALATQYSIDYVETLEFTDADNILGNIPVQFLKRYKIAPYTVKKNTIK
                     IAINDPLLIQPLDNIKIMYDSFKIIPVLSTEKEILKIINNHFDVLKENSTTSVIENLE
                     ESDFEILSSPITETEDILDMANEAPIIRLVNTVIKQAIEDRASDIHIEPFEKDLIIRF
                     RIDGILYNMFTPPKKYQAAIISRIKIMSNLNIAENRLPQDGRIQLKVGGKDIDIRVSV
                     FPTYYGERIVLRLLNKSDMKFDLDSLGFSKDSLSIFNELIKKTHGIILVTGPTGSGKT
                     TTLYSVLTRLNTYDVNILTVEDPIEYQLHGVGQMQVKPKIDLTFAAGLRSILRQDPDI
                     IMVGEIRDLETAEIAIQAALTGH"
BASE COUNT         1377 a          570 c          644 g         1026 t
ORIGIN      
        1 gtaaaaagtt tttctgaata tcaatcgatg ctggattcta atttttttaa aatagccatg
       61 gccgatagtt ccggtaattc agtagtcagc agcgaaataa atgaacttgt tgtattgggt
      121 actatttctg gaccagctac tattgcacgt gctttaataa aaagacaatc agaaagctct
      181 gcacaaattt ataagattgg catggacgta tatgggttca aacttattaa aattgataat
      241 gcaaaagttt atttaaaaaa agatggcaag attgagatca ttgatatgtt tgctaaagaa
      301 aaacctcagg aggcattatc ttcacagcaa acaatgcaac aaggtacaac ttctgcaaac
      361 agagttgcca aatcaataag taaagctgaa atccaacaaa aagtactaaa caatcttgac
      421 aatgctttaa atggtgttag agctggtcca tacagagttg ggggtaaaat agaaggattt
      481 aaactatttt cagtagctcc atttaatata ttatattcta tgggagcacg tagtggtgat
      541 atagtaaaaa gaataaatgg acaaccaatc gatagcaccg aaaaattaat gaaaatgtgg
      601 gaaaccctta aaactgacac acgctttgtt atagaattag aacgttcagg aaagatgata
      661 acctatgatt taaatgtttc agagtaatta tactaacaat ataaaaagat tggtgattca
      721 atgctaaaca aaattttttg catttataaa tatttcctgt gttcagcact tgttatgatt
      781 tttttcacaa taatttatgc gcaacaggaa aatataactc cggataaatc tgttgattca
      841 caaatacagg cagaaattac tccaaaggct gttgaagcaa atcctcaaag tcaggaaact
      901 gtttctcaaa atgaacaaaa ggatgtaaca caaaaaccag aaataaaaat tgaaaaattt
      961 aaaggcaaaa aaaaagatga ggaaaaatat tttacgctga attttaaaga tgttgaactc
     1021 gccgaatttt taagtgtgat gagtcagctt ataggtaaaa atatagctct tgatgaaagg
     1081 gtccgtggta aaataactat ctcatcagtt aaaaaaattc cagttagaga cgcctatgat
     1141 attatgaaag ctatcctgga gttaaaagga tttgcagtgg tagaatcaga aaatttaatt
     1201 aaaattatcc ccataaaaga tgcaatcaag aaaaatgttg aagttattat agatactaca
     1261 aaaaaacaag ttatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctgat
     1381 gcagaaaaat tagcagcagt tttatcaaga ataccttttg ctgaatacgc tgcaattagc
     1441 gttggggaaa cacaaacacc acaaacagtt gaagctataa agaaagcaca acaagccgtt
     1501 caggctaaat catcgataac tgacaaacaa aagctttcaa tcatagccaa caaagaaacc
     1561 aactccctta tcattacggc aaaacctgaa gaatttaaaa tgataaaaaa tattattgat
     1621 cagcttgata ttgttcgtga acaggtactg atagaagctt tgattattga agttgtagcc
     1681 gatagtggtt ggggatttgg tatagactgg atgataggcg accagagtgg cacccatcta
     1741 tatggcggtt catccataca gggaactcca ccatcatata cactgccatc gggaatgact
     1801 ggcaaagaag ttgcagtgcc tttatcaaca ggcttccaga ttggatattt atctgataaa
     1861 tcaatgttag gatttgcact tctaaatgca acaggtgcag aaaaacatta caatatacta
     1921 tcgacccctc agattttaac taccgataat caggaagcag aaataaatgt aggagaagaa
     1981 atacccgttc ccacaaataa caggattagc gacacgggtg ttcagtttta tacctatgaa
     2041 tacaaatcgg taggtgtgaa attgaaaatt actcctcaca taacatccga aaacagcata
     2101 actctggatt tgtatcagga ggtaaattca gtacttggac aaacaacagt actggaatct
     2161 ggctctataa tacctcccac attaggaaaa cgcgatatca aaacaaaaat aaatgttgct
     2221 gatagcaaaa caattgtagt tggaggatta attaccaata ataaaactga agaagaaaca
     2281 aaagtgccta ttttaggtga tataccactt ttaggatggt tttttaaacg aaaggttgtt
     2341 gaatacaaaa agacaaacct tttagttttt attacccctc atattgtgac taaacaagct
     2401 aaaatggatg ccttatccga acaaaaacga gaggagcaac gaagattaca attagaataa
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tagaataact atgataggga
     2581 ttaaaacaaa aaaattaggt caaattttaa ttgataaacg gttgatttca caggaagatc
     2641 ttcaggatgc attgttgtct caacaaaata cgcctttaaa aattggtcag atattgataa
     2701 aaaaaggatt gattgctgaa gatgaagtat tgaaagctct ggcaacgcaa tattcaattg
     2761 attatgttga aacacttgaa tttactgatg cagataatat acttggtaat attccagtac
     2821 aatttttaaa acgttataaa atcgcacctt atactgttaa aaaaaatacc attaaaattg
     2881 ctatcaacga tcctcttctt atacagccgc tggataacat taaaataatg tatgacagct
     2941 ttaagatcat tccggtgtta tctaccgaaa aagaaatact aaaaatcatt aataatcatt
     3001 ttgatgtatt aaaagaaaat tcaacaacat ccgttattga gaatttagag gagtctgatt
     3061 ttgaaatatt gtcctcgcct ataactgaaa ccgaagatat tttagacatg gccaatgagg
     3121 cacctattat tcgccttgtc aatactgtaa taaaacaagc aattgaagac agggcaagcg
     3181 atatacatat tgaacctttt gaaaaagatc tcatcatacg ttttagaatt gatggtatat
     3241 tgtataatat gtttacgccg cctaaaaaat accaggctgc aatcatttca cgaattaaaa
     3301 ttatgtctaa tcttaacatc gcagaaaacc gattaccaca ggatggcaga attcaattaa
     3361 aagttggcgg caaggatatt gatatccgtg tttccgtgtt tccaacgtat tatggtgagc
     3421 gtatagtgtt acggttgctt aataaaagtg atatgaaatt tgatcttgat tcattaggtt
     3481 tttcaaaaga ttcattatca atattcaatg aattaataaa aaaaacacat ggaataattt
     3541 tggtaacagg tccaacggga agtggtaaaa caactacact atacagtgta ttaacacgat
     3601 tgaataccta tgatgtaaat atattaactg tagaagaccc aattgaatac cagcttcacg
     3661 gtgttggaca gatgcaggta aaacctaaga ttgatcttac atttgcagca ggtttacgct
     3721 caatattacg tcaggaccct gatataatca tggtaggtga aatacgtgac ctggaaactg
     3781 ctgagattgc cattcaggca gcactcacag gccacag
//
LOCUS       DAOSOX010000215         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24104_length_3807_cov_9.571162, whole genome shotgun sequence.
ACCESSION   DAOSOX010000215 DAOSOX010000000
VERSION     DAOSOX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24104_length_3807_cov_9.571162"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLX22_11295"
     CDS             complement(<1..1072)
                     /locus_tag="PLX22_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT20521.1"
                     /translation="MTVKCNTIFKTILSVFMYLVLFIITATGTIYAQNPLTLKEAIDI
                     ALKNNNTYKVALLKVEENRYKVRESWGMLWPKLSTDVSYTRQWYEAGFQSQVEGQYNV
                     KIINGEISINPGTFYNTLQSSRNNFIASEHDARRVKADTTVTTLRLYYQILLTKELIA
                     MHEGSLKALEENLRIVTIGYQKGTFSKLDFLRAKVAYNNEKTQLINAKNDYLTVCATF
                     NIHLNREINEALILDESVLKVHDEDLQYLVLEEAKKNEIVGQLIALALKNRPELLQLT
                     YSKTALEYATDATSSVYLWPSFFVSGSYGSSKNISKDGGGGLTPTGDPTIDPILLAMS
                     QGMSDSFAPSGWNDNWMITFGAT"
     gene            complement(1069..1809)
                     /locus_tag="PLX22_11300"
     CDS             complement(1069..1809)
                     /locus_tag="PLX22_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT20522.1"
                     /translation="MVIDYMEEFKYSKETRTVILQAAKTEFAEKGYDGARLSLIAKRA
                     GVNQALIHYYFSNKEKLYLEVLQYLFHHGEISIIREHLKDVTLNPKEALFTAIYILVY
                     LHHNAIDPEINKIISWELAQGGDKFKMLLTDYMLPKLNMLSQVIQEGIESGDFETDDP
                     FLVVFNLTNMVLSFENSKQYLKDTPWYDRFYGGDNGKKLFEFALKSTFKSLAPKHKAC
                     EIPQINEDLKHKLEEIMQKLKNTSEHEK"
     gene            2336..3493
                     /locus_tag="PLX22_11305"
     CDS             2336..3493
                     /locus_tag="PLX22_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT20523.1"
                     /translation="MQPSNGIVYEFFIFCFKVASLIMRHKAHLKGKEYLKKIPHLVIF
                     APTHDSYFEVPSLSKVYYSLKPRPHFVVMAKKDFLSGNYLASNFGEKNKLLYYFFYFL
                     DKTGLPKAFFKKMKLIDIPRPFIEDFDNKNQVKHEIHSQMVKFKHSIHSGFSTLIFPE
                     GTTWSFGGLKNLRSGIFQLVDSTYRHFRQKLYILPINVKVDRCIAGKKDIFINVGEPF
                     FMLGDKEKFLDRVSEILKSLHTITFSQIAATYLQRMAMAVENIDKAIVLKKEELASQL
                     DTVINDIIVLVKEGKLPGIDEYLLDSNYRHKKLKRFLKFCIKKGYIEKIDGQYMINAQ
                     RVLASFHIKSYRKENPVGFTANEFLSINYNCIMTIYEKYCNNYKVITAPIQ"
     gene            complement(3499..>3807)
                     /locus_tag="PLX22_11310"
     CDS             complement(3499..>3807)
                     /locus_tag="PLX22_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20524.1"
                     /translation="YVIYKNTMIDIAPYISKFMTLPVNTKLIPVAYVAPVVLVIIAVF
                     LFKQTAGIYTRWLALVILLTSVALFYSLDPSFIKNLLSTPQVQQNIKEGVKRGIRNIH
                     "
BASE COUNT         1190 a          651 c          694 g         1272 t
ORIGIN      
        1 aggtagcacc aaaggttatc atccagttgt cgttccagcc actgggagca aaactatcgg
       61 acataccctg actcatagcc agtaaaattg ggtctatagt tgggtcccct gttggcgtaa
      121 gtcctccccc accatccttt gatatattct tgcttgagcc atagctaccg ctcacaaaaa
      181 aacttggcca tagataaacg gatgaggttg catcggttgc atattccagt gctgtcttgg
      241 aataagtcaa ttgcaaaagt tctggacgat ttttcaaagc caatgcaata agctgtccaa
      301 cgatctcatt ttttttggct tcttcaagaa caaggtattg taaatcttca tcatgcacct
      361 ttaaaacaga ttcgtccagt ataagggctt catttatttc ccggtttaga tgaatattaa
      421 atgtagcgca cactgttaaa taatcatttt ttgcattgat cagctgtgtt ttttcgttgt
      481 tgtaggctac cttggcgcgt aaaaaatcca attttgaaaa agttcccttt tgatagccaa
      541 ttgtcactat acgtaaattt tcttccaatg cctttagtga tccctcatgc atggctatca
      601 attcctttgt cagcaatatc tgatagtaga gcctcagcgt tgtgacagtt gtatcagcct
      661 ttaccctcct tgcatcatgt tcgctggcaa taaaattgtt gcgtgaggat tgtaatgtat
      721 tataaaatgt accgggattt atagatatct ccccattaat aattttgaca ttatattgtc
      781 cttcaacctg cgattggaaa ccggcttcat accattggcg ggtataggaa acatccgtag
      841 aaagttttgg ccataacatc ccccagcttt cgcgcacctt atagcggttt tcttccacct
      901 tcaataatgc tactttatag gtgttattgt ttttgagtgc tatatctata gcttctttta
      961 atgttaatgg attttgtgca tagattgtac ctgttgcggt aattataaac agtaccaaat
     1021 acatgaatac tgatagtatt gttttaaaaa ttgtgttgca tttaactgtc atttttcatg
     1081 ctccgaagtg tttttaagtt tttgcataat ttcttcaagt ttatgtttta aatcctcatt
     1141 tatttgcggt atctcacaag ctttatgttt gggggctaat gacttgaatg tgctttttaa
     1201 ggcaaactca aataactttt ttccattatc accgccatag aagcgatcat accatggtgt
     1261 gtctttgaga tattgtttgc tattttcaaa tgataaaacc atattggtaa ggttaaagac
     1321 aacaagaaaa ggatcatcag tttcaaagtc cccgctttct ataccctcct gaattacctg
     1381 cgacagcatg ttgagtttgg gtaacatata atcggtaagg agcattttaa atttatcgcc
     1441 accctgcgct aattcccatg aaataatttt atttatttca ggatcaatgg cattatgatg
     1501 cagatatacc agtatgtaga ttgctgtaaa aagagcctct ttaggattta atgtaacatc
     1561 tttcagatgt tcccggataa tgctgatttc tccatggtga aacaggtatt gcagcacttc
     1621 aaggtatagt ttttccttat tggaaaaata atagtggata agtgcctggt ttacccccgc
     1681 tcgcttagca attaaactta aacgggcacc atcatagcct ttttcagcaa attccgtctt
     1741 tgcagcctgc aatatgacag ttcgagtttc ctttgaatat ttaaattctt ccatgtaatc
     1801 aataaccata cttttaagtt gtcatccatg acattaatca attattttaa ttaaaaaatt
     1861 aaattgatta gttaaatata tagcaattct tgtcaatagc aaaataaaaa aataaaaaat
     1921 tttaaaaaaa tattgtatta taaatcaaac gataacacct atagaatatt attttaggca
     1981 attttagggt agttgcttat ggcagagcat tcatccagcc ataagggatg aacatattta
     2041 gcgtctatca ttctgagctg aagccgtctg tgtggagcga agtcgaagaa aaaaatctca
     2101 ctgtggcatg gggatgtcat tctgaacgaa gtgaagaatc tctgcttttt atgaaaggat
     2161 agattcttgg ggactgggtc tctcagaatg acacggtatt ctattttaag aggaatgcaa
     2221 tcatttgaag atgaaaacac catgcttttc attatacttt aatgtatttt ttactattga
     2281 caattgctgg tgaatagttt atttttatac cagtaaagtg gtttgaggta tgtatatgca
     2341 gccatcaaat ggcatagtct atgaattttt tattttctgt tttaaagttg cttcattgat
     2401 tatgcggcat aaagctcatt taaaagggaa agaatatcta aaaaaaatcc cccaccttgt
     2461 aatatttgcc cctacgcatg atagctattt tgaggtgcca tccctttcaa aggtatatta
     2521 ttcattgaaa ccacgtccac actttgtggt aatggcaaaa aaagattttt taagtgggaa
     2581 ctatctggca tcaaattttg gagaaaagaa taaactgctc tattattttt tttatttttt
     2641 agataaaaca gggttgccaa aagctttttt caaaaagatg aaactgattg atattccacg
     2701 ccccttcata gaagattttg acaataaaaa tcaggtgaaa catgagatac actctcagat
     2761 ggtaaagttt aaacattcta tacatagtgg tttttctacg cttatatttc cggaagggac
     2821 aacgtggagt tttggaggtt taaaaaattt acgcagtggg atattccagc ttgttgatag
     2881 tacctatcgc cacttcaggc aaaaattata tatactgcca attaatgtta aggttgaccg
     2941 ttgcatagcc ggtaaaaagg atatttttat aaatgttggg gagccctttt ttatgcttgg
     3001 ggataaagaa aaatttttag atagagtttc tgaaatttta aaaagtttac ataccataac
     3061 cttcagtcag attgctgcaa catatctcca gcgcatggca atggcggttg aaaatattga
     3121 taaagcgata gttcttaaaa aagaagaatt ggcatcacag ttagatactg tcatcaatga
     3181 tattattgtg ctggtcaaag aagggaaact gcccggtatt gatgaatacc ttttggatag
     3241 taactatcgc cacaaaaaat taaaacggtt tttaaaattt tgcatcaaga aagggtatat
     3301 tgaaaagatt gatggacagt atatgatcaa tgctcaacgg gtgctcgctt catttcatat
     3361 caagagctac aggaaggaaa atcccgttgg atttaccgct aacgaatttt tatccattaa
     3421 ttataattgt ataatgacaa tatatgaaaa gtactgtaat aattacaaag taattacagc
     3481 gcctatacag taacaggttt aatgaatatt gcgtatcccc cgttttacgc cttctttgat
     3541 gttttgctga acctgcggag ttgaaagtag atttttaata aatgaagggt ccaggctata
     3601 gaaaagcgca actgatgtta acagtattac aagcgctaac cagcgggtat agatacctgc
     3661 agtttgctta aacaaaaata cagcaataat gacaagaaca accggtgcaa catatgcaac
     3721 aggtataagc tttgtattaa ccggcagtgt cataaatttt gatatatagg gggcaatatc
     3781 aatcatggta tttttataga taacata
//
LOCUS       DAOSOX010000216         3802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24146_length_3802_cov_8.071524, whole genome shotgun sequence.
ACCESSION   DAOSOX010000216 DAOSOX010000000
VERSION     DAOSOX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24146_length_3802_cov_8.071524"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /gene="glmS"
                     /locus_tag="PLX22_11315"
     CDS             complement(<1..1054)
                     /gene="glmS"
                     /locus_tag="PLX22_11315"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOT20525.1"
                     /translation="MCGIVGYIGSKNVAEVLINGLRRLEYRGYDSVGIATLIGDELII
                     QKQKGKVIDLIKRLDYEKFNGCTVGIGHTRWATHGEPNDINAHPHCDCKGDIALVHNG
                     IIENYGAIKKVLIEHGHTIQSETDTEVLVHLIEYYHQTMSLVDAVQKTLTKVEGTYGI
                     VIISKKEPNKMVAARNGSPLILGIGDGEMIVASDASAIVEHTRNVMYVSDGEIVEITK
                     DTVRTFDLRKNELEKHSNKIDWDIAYIEKQGFPHFMLKEIFEQPETIQNAFRGRIVLD
                     EGKTRLDGLRLTEDELNSIKRIIFIACGTSWHAGLIGEYLIEEYARIPVEVEYASEFR
                     YCSPILEKGDLVIVISQ"
     gene            complement(1285..1455)
                     /locus_tag="PLX22_11320"
     CDS             complement(1285..1455)
                     /locus_tag="PLX22_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT20526.1"
                     /translation="MAYKITEDCTMCGTCASECSSQAISEGNDRYVIDNDICTECGTC
                     IDVCPAEAIVEV"
     gene            1863..2855
                     /locus_tag="PLX22_11325"
     CDS             1863..2855
                     /locus_tag="PLX22_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20527.1"
                     /translation="MKFISKICLIMLIVFTTSTIFAGQWVYKPMSINAQKGDVILSTS
                     PGFIMDLLAILGCYWSHSGMTVDNGFNIRHNTMYVSDIPIEYNKIWFIQTTPKRMNPD
                     KLSNGMPGILTENIDTTYNVTKNFAAAGGAVLKPTSADEALYRQYLVNAADKMLYVKG
                     YYRVNAYVNMYQLDYVNYYIKGRGNHCSGTCWYANYFAGKTMNVAYIPPSLVSQCGYS
                     LYNSVKNMVRDEAGGFGSFIIDIEGLFGTGADEKIANQIVNTFGWDRSTDTSSYWKSY
                     INKISATANAPDHLLLYTYINPSGKNTGVQTTSSSYYGQVDPLVITSGYYYWVD"
     gene            3073..>3802
                     /locus_tag="PLX22_11330"
     CDS             3073..>3802
                     /locus_tag="PLX22_11330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT20528.1"
                     /translation="MKRIYIIIGIIAAVMLIFIFLIHSNNDKLKNDDPSKNQSLLNIF
                     QFMSQQEQSEELPPVDPEKVETYEIEQANFIKDIILEKNPVCAGEDFKVTVIAKNPFG
                     SDAHLVYRISNKLGNPAIMRFNKAGQREFYVTVRDEGKHIDMRKVQITVVECPDKPLV
                     VLSARLHNLEPESAIFEVTSQKGLSGKCTYEWDFGDGTYATTQNGYITHSYASRDQKG
                     LQSSFLVKVTVADANNITATGRNSI"
BASE COUNT         1219 a          718 c          614 g         1251 t
ORIGIN      
        1 attgacttat aacaataaca agatcacctt tttcaaggat tgggctgcaa tatctgaatt
       61 ctgaggcata ttcaacctca acaggtattc gggcatattc ttctattaaa tactctccaa
      121 taagtcctgc atgccatgag gtaccacagg ctataaaaat aatacgtttt atactattca
      181 actcatcttc agtaagccgt aatccatcca aacgggtttt accctcatcc aatactatcc
      241 gtcccctgaa cgcattctga attgtctcgg gttgctcaaa tatttccttc agcataaaat
      301 ggggaaatcc ctgtttttct atatatgcaa tatcccaatc aattttattg ctgtgctttt
      361 ccaattcatt ttttcgtaga tcaaatgttc gtacagtatc ttttgttatt tctactattt
      421 caccatctga aacatacatg acattccgtg tatgttcaac gattgcactg gcatcagaag
      481 ccactatcat ttcgccatcg ccaataccaa gtatcagcgg actcccattc ctggcagcta
      541 ccattttgtt gggttctttt tttgatataa taacaatacc ataggttcct tcaaccttgg
      601 ttaatgtttt ctgtactgca tcgacaagtg acatggtttg gtgatagtat tcaatgagat
      661 gcactaaaac ttcggtatct gtttcacttt gaatagtgtg accatgttca atgagtactt
      721 tttttatagc accataattt tctatgattc cattgtgaac caacgcgata tcacctttac
      781 agtcacagtg tggatgggca ttaatatcat ttggctcacc atgtgttgcc catcgtgtgt
      841 gccctatacc aactgtacat ccattaaatt tttcataatc taatctttta ataagatcaa
      901 taaccttacc cttttgcttc tgaattatca attcatctcc tatcaacgta gctatcccta
      961 ctgagtcata accacgatat tctaatcttc tcaaaccatt gataagaacc tcagcaacat
     1021 ttttactacc tatatagcct acaataccac acatgaaaaa ctcccaaaaa aagattttac
     1081 tataaagaag aattatacca tgccattctg aatgacgaag ccctttatgt cattctgaga
     1141 aagcgttagc acccaaagaa tctttccttt caacagcttg attttcttca tcagcctcag
     1201 agtgacagca cactatattt catcgtttaa aaaaagcaaa gcatttttat attaaaaaaa
     1261 gtggggctgc atgccccact atcatcatac ttccactatt gcttcagctg ggcacacatc
     1321 aatacatgtg ccgcactctg tacaaatatc gttatctatg acataacgat catttccttc
     1381 agaaatagcc tgagatgaac attcagaagc acatgttcca cacattgtac aatcttcagt
     1441 aattttataa gccatggact cctcctgtaa agtttaaata aaaaccattc aactgattac
     1501 attattacaa aaattatttt tatgtcatat atgtagcaca tcagaatttg taaatcaaaa
     1561 ataatcgtat gtatcctctt gttaccaaca tcatatatca cttactatta ttacaataaa
     1621 gctttcactt tatttttaat tgaattatat aaatcatttt tttatgtaat acaattaaac
     1681 aaatagttaa aaaaattttt tattttatac ctaatagtcg gtttatgaat tgactttttt
     1741 ttgtaatcaa ctcattttag attcgagggt gtttattatt aaaaagtgag tgataattca
     1801 ctcacttttg atgagcacaa ttctatgatt ttgaattata tattgatcaa aaggaggttc
     1861 caatgaagtt tatttcaaaa atatgcctta ttatgcttat tgtgtttaca acatcaacaa
     1921 tctttgcagg gcaatgggtt tataaaccaa tgagcattaa tgcccagaag ggagatgtta
     1981 ttctttcaac cagtccggga tttattatgg atttacttgc aatattgggt tgctactgga
     2041 gccatagcgg gatgactgtt gataatggat ttaacattcg ccacaacacc atgtatgtta
     2101 gcgatatccc tattgaatac aacaaaatat ggtttattca aacaacaccc aaaagaatga
     2161 atcctgataa attgagcaac ggcatgcctg gcattttgac tgagaatatt gatactacct
     2221 ataatgtaac aaaaaatttt gctgctgccg gtggtgctgt acttaagcca acttctgcag
     2281 atgaagcact gtaccgacaa tatcttgtta atgctgctga caaaatgttg tatgtcaagg
     2341 gatattaccg cgttaatgca tatgttaaca tgtaccagtt agattatgta aattattaca
     2401 taaaagggcg aggcaatcac tgctcaggca cttgctggta tgctaattac tttgcaggca
     2461 aaacaatgaa tgttgcctat attcctccat cattggtatc acaatgcggc tatagtttat
     2521 acaactcggt caaaaatatg gtccgtgatg aagccggcgg atttggatct tttattattg
     2581 atattgaggg gctttttgga actggtgctg acgaaaagat agccaatcag attgttaata
     2641 catttggctg ggacagatca actgacactt catcatactg gaaaagctat attaataaaa
     2701 tatccgcaac cgccaatgca cctgaccatc tgttgcttta tacctatatc aatccatcgg
     2761 gaaaaaatac cggtgtgcaa actacctcct cttcatacta tggacaggtt gatcctctgg
     2821 ttattacctc cggatactat tactgggtgg attaatctgc tataatacaa catcccgggt
     2881 tttttatgcc cgggattttt ttattataac aaattcattt atagttagtg gcagttgttc
     2941 atatgatcaa tgttatagtg aaagaatgat gtgagataag tttttatgag taactatcga
     3001 acaaaatttt tgttgatatt ttttatttaa atccttatct tattttaaaa tttataccat
     3061 caggtaaaac gtatgaaacg tatatatatc attattggaa ttattgccgc agtcatgtta
     3121 atatttattt ttttgataca cagtaataat gataaattaa aaaatgatga tccttccaaa
     3181 aatcaatcgc tattaaatat ttttcaattc atgtcccagc aggaacaatc tgaagaattg
     3241 ccgccggttg atcctgaaaa agttgaaaca tatgaaatag agcaggcaaa ctttataaaa
     3301 gatatcatac ttgaaaaaaa tcccgtctgt gccggcgaag attttaaagt aacggtcatt
     3361 gcaaaaaatc catttggatc agatgctcat ctggtgtatc gcataagcaa taaattgggc
     3421 aatcctgcaa tcatgcgatt taacaaagca ggacagcgag aattttatgt aacggtgcgt
     3481 gacgaaggta aacatataga tatgcgcaaa gttcagatta ctgttgtaga atgccctgat
     3541 aaaccgcttg tagtattaag tgcacgttta cacaatttag agccagaatc tgcaatattt
     3601 gaagtaacct cgcaaaaagg gctatcaggg aaatgcacgt atgaatggga ttttggcgat
     3661 ggtacttatg ctactactca aaacggatat atcacccata gctatgccag tcgtgatcaa
     3721 aaaggattac aatcaagttt tttggtaaag gtaacagttg ctgatgcaaa caatattacc
     3781 gcaacaggca ggaatagtat tt
//
LOCUS       DAOSOX010000217         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24339_length_3779_cov_7.233083, whole genome shotgun sequence.
ACCESSION   DAOSOX010000217 DAOSOX010000000
VERSION     DAOSOX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24339_length_3779_cov_7.233083"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1946
                     /locus_tag="PLX22_11335"
     CDS             28..>1946
                     /locus_tag="PLX22_11335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT20529.1"
                     /translation="MNGPRIVVHGNAQDGVGNTMNNGVIVIHGKAGEIPGHSMRGGII
                     IIRDDAEYRAGIHMKEYEDQIPLIIIGGTVKDYCGEYMAGGRIVVLNRLNDNYPAGND
                     VGTGIHGGVIYIRGNVDSRRLGVGAVFNTLDDDDKNFLQYALSLFKQEFPYAELSNIS
                     IDDFLKITKKGHRPFANLYSPAMNIKTKKPIHLNLTPPCTYACPTGIPTPVFLNLIKD
                     GKVKDAQMLMDEYTPFRMSVCGTVCPAPCMEACSRNMIDGPMDIPYLAREYYPDFNPT
                     VYGKPNNKKVAVIGAGPAGLSAAWQLARRGYTVTVFEKTSDIGGKVRLAIPKDRLPDE
                     ILGRDLERIKSLPIEFKVNTRVDAELFSRIYQDNDLVLIATGSYISRKLLVKGGERIV
                     SGLEFLMGINEGNPMDLTDNDVVIIGAGNVGMDIACESWRHGAKTVTAVDIQKPLSFG
                     KEREMAERLGTAILWPRSIDYIDDAMIYFTNGESMHADAIFISIGEKPDISFLPETVM
                     VNERGYIVTADKSFQTSDSKVYACGDIVTTGLITDAIGMGRLAAMEIHAYCEGEDFIY
                     PGKTPVSRRRINTAYFGGETHEIDRCISCGTCIFCDKCVQICPQGAITRNGEIFTIDS
                     ELCTACYTCVNVCPRG"
     assembly_gap    1947..2046
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2047..2522)
                     /locus_tag="PLX22_11340"
     CDS             complement(<2047..2522)
                     /locus_tag="PLX22_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT20530.1"
                     /translation="MAPRNKGIQLMINKIEKAPDIPSLVVSQIIELISSGVLKPGDRL
                     PSELEMTRRFGISRISLREAMKLLEAKGYIESQGRKGKYVKSVINDAIRTPIEGLIQI
                     DHKKIWELLEVRRIIDAEAAALAALRANEEQRRKLNSFKTQIELMGVENLIQKREGG"
     gene            2959..>3779
                     /locus_tag="PLX22_11345"
     CDS             2959..>3779
                     /locus_tag="PLX22_11345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT20531.1"
                     /translation="MKIIISGPKGCGKSTVGKRIAEEIGVSFYETDEMIESMFADKHG
                     TRKTCRMIVREFGEEHFREWETAAIEKIALLDWCVVATGGGTMLLPINRALLRRDSII
                     VLLKASIDVLWERMQITGLPPFLQGDDAYHNYCQRVMKVYEATEHISDVAVEVTHDNE
                     ATVHKEIIEQISFVMGQWMFAPNTFGQVLRITTFGESHGAAVGVVIDGLKPDIPIDEN
                     DIQKQLDKRKPGQSTISTQRKEADHVAIVSGIFEGKTTGTPVCMIVYNKDKDSMA"
BASE COUNT         1103 a          641 c          871 g         1064 t
ORIGIN      
        1 actccgggac aggatctggg tgcttttatg aatggaccac gaattgtggt acatggtaat
       61 gcacaggatg gtgttggaaa tacgatgaat aatggggtga tagttattca tggaaaagca
      121 ggggagattc caggacattc catgcgcggc ggtattatca tcatccggga tgacgctgaa
      181 tacagagctg gcattcatat gaaagagtac gaggatcaga ttccactgat catcataggt
      241 ggaactgtaa aagattattg tggtgaatat atggcaggtg gcaggatagt tgtattgaac
      301 aggctgaacg ataactatcc agcagggaat gatgtgggta ctggcatcca cggcggagtc
      361 atctatatac gaggaaatgt tgacagccgc aggctgggag ttggtgcagt gtttaataca
      421 ctggatgacg acgataaaaa ttttttacag tacgcactat cactttttaa gcaggagttc
      481 ccctacgcag aactatcgaa cataagtatt gatgattttt taaaaataac caaaaaagga
      541 catcgtccat ttgcaaacct gtattcaccg gcaatgaaca tcaaaacaaa aaaaccaatt
      601 catcttaacc tgaccccgcc atgcacctat gcatgtccaa ccggaatacc aacgccggta
      661 tttttaaatc ttataaagga tggtaaggta aaagatgcac agatgcttat ggatgagtat
      721 acgccattca ggatgtcagt atgcggtacg gtttgccctg cgccctgcat ggaagcctgc
      781 agtaggaaca tgattgatgg accaatggat attccctatc tggcacggga gtattaccct
      841 gattttaatc caacggtata tggaaaacca aacaataaaa aagttgctgt tattggcgca
      901 ggccctgctg ggctttctgc agcatggcaa ctggcgcgaa gagggtatac agtaacagta
      961 tttgaaaaga ccagtgatat tggtggcaag gtgagattgg caatcccaaa agacaggttg
     1021 ccggatgaga tattgggaag ggatctggaa cgtatcaaaa gtttgccaat agaatttaag
     1081 gtcaatactc gtgtggatgc agagctcttt tccagaatat accaagataa tgatctggtg
     1141 ctcattgcaa caggtagtta catttcacgt aaacttttgg ttaagggtgg tgagcgcatt
     1201 gtttcaggac tggaattttt aatgggcata aatgaaggca accccatgga tttaaccgat
     1261 aacgatgttg tcattattgg tgccgggaat gttgggatgg atatagcctg tgagtcatgg
     1321 cgtcacggtg caaaaacagt tactgctgtt gatattcaaa agccgttatc ctttggtaaa
     1381 gaacgcgaga tggcagaaag gcttggtact gccatactct ggcccagaag catagattat
     1441 attgatgatg ccatgatcta ttttaccaat ggcgaatcga tgcacgccga tgctattttt
     1501 ataagcattg gtgaaaagcc ggatatttca tttttacctg aaacagtaat ggtaaatgag
     1561 cggggatata ttgtaacagc agataaatca tttcaaacct ctgatagcaa ggtttatgct
     1621 tgtggcgata ttgtaacgac aggtttaata acggatgcta ttgggatggg caggttggct
     1681 gcaatggaaa tacatgccta ctgtgaaggt gaagatttta tctatccagg aaaaacaccg
     1741 gtgtcacgac gaagaattaa cactgcctat tttggaggtg aaacacacga gattgataga
     1801 tgtataagct gtggcacctg tattttttgc gataaatgtg tgcagatatg tccacaggga
     1861 gcaataacac gaaatggtga aatttttacc attgattcag aattgtgcac agcatgttat
     1921 acctgtgtta acgtttgccc gcggggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnccac cttcacgttt ttgaatcaaa ttttcaacac ccataagttc aatctgggtt
     2101 ttgaatgaat taagcttacg cctttgctct tcgttggcgc gcaatgcagc taaagcggct
     2161 gcctcggcat ctataatgcg tcttacctct aaaagttccc atattttttt atggtcaatc
     2221 tggatcaatc cttcaatagg agttctaatt gcatcgttga tgactgattt tacatatttt
     2281 cctttgcgtc cctgtgactc gatatagcct tttgcttcaa gcaatttcat tgcctcgcgc
     2341 aaggatatac ggctaatacc aaatcttctg gtcatctcta attctgaagg cagcctgtca
     2401 cctggcttta acacgccgga tgaaataagt tcaataatct gtgaaactac cagactcggg
     2461 atgtcagggg ctttttcaat tttgttgatc atgagctgaa tgcctttgtt acgtggtgcc
     2521 attgatgtgg tctcctttat agggtagtgt agtgtttttc atagtgccat gaaatgatat
     2581 atcagaatga tcattactcc aaagtattat tgtattacat attataatat taatgatatt
     2641 gtgtcaacac aattaatata gcaaagttgc atgactatct atacaaaata aaataatttt
     2701 aattaaagca aaaaatatgc attattatat catgaccata caaaatatct aaaaattttg
     2761 ttgatttgtt tattgtcata caataggcaa catcgtattt gataaaatat gggttgaata
     2821 gtatgaatgt gttgcaatag ttttttttgg cgatagtaac tttttattga ctgatgcttg
     2881 ttaattgcat ttattgttat aattatattc cgggttgtta ccattgcctg caataatcta
     2941 tttttgggaa aaacgtacat gaagattatc ataagcggac caaaaggttg cggaaaatca
     3001 accgtgggga aacgaatagc tgaggagata ggtgtctcgt tttacgaaac cgatgaaatg
     3061 attgaatcca tgtttgctga taagcatgga acacgtaaaa catgcaggat gatagtgcgg
     3121 gagtttggtg aggagcattt cagagaatgg gaaacggcag ccattgaaaa gattgccctg
     3181 ctggactggt gtgtggttgc taccggtggg ggtaccatgc tgttgcccat caacagggca
     3241 ttattgcgca gggatagcat tattgtttta ctaaaggcat ctattgatgt attgtgggag
     3301 cgtatgcaga taaccgggct cccgccattt ttacaggggg atgatgcata ccataactat
     3361 tgtcagcggg ttatgaaggt ttatgaagca acagagcata tcagtgatgt tgccgttgaa
     3421 gtaactcacg ataatgaagc aacagttcat aaggagatta tagagcagat ttcatttgtt
     3481 atggggcaat ggatgtttgc tcccaatacc tttgggcagg tattgcgaat aaccaccttt
     3541 ggcgaaagcc atggggctgc agtgggtgtg gttattgatg gcttaaaacc ggacatccct
     3601 attgatgaaa acgatattca aaaacagctt gataaacgaa agcccgggca gagcaccata
     3661 agcactcagc gcaaagaagc tgaccacgtt gcaattgtat cagggatatt tgaagggaaa
     3721 acaacgggca ctcctgtatg tatgatagta tataataaag ataaggattc catggcgta
//
LOCUS       DAOSOX010000218         3771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24404_length_3771_cov_9.726588, whole genome shotgun sequence.
ACCESSION   DAOSOX010000218 DAOSOX010000000
VERSION     DAOSOX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24404_length_3771_cov_9.726588"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2082
                     /gene="carB"
                     /locus_tag="PLX22_11350"
     CDS             <1..2082
                     /gene="carB"
                     /locus_tag="PLX22_11350"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOT20532.1"
                     /translation="MRSVGEVMSIGKNYKEAFQKAIRSLEIGRHGPGFAKDFNKLPLE
                     ELIRLLSTPSSERHFIMYEALRKGADIAILKERTQIKEYFIQQMKELVDLENEMLPFA
                     KKSLPDSLLKTAKEWGFSDRYLSKILELPEDAIRKQRISLGVVERWEAVPVSGVEDAA
                     YYYSTYNAPDTVSVSHRKKIMVLGGGPNRIGQGIEFDYCCVHAAFAIKEAGYEAIIIN
                     CNPETVSTDYDTADKLYFEPLTVEDVLSIYEKEKPEGVIVQFGGQTPLNIAGELVKAG
                     VKILGTSVDSIDLAEDRDRFRKVITKLSIPQPESGMASSLEEALSIANQIGYPLMVRP
                     SYVLGGRAMEIIYDEEMLKQYVKAAVEVTPERPILIDRFLVDAIECEADAICDGENVF
                     VPSVMEHIELAGVHSGDSACAIPPDNIKKEHIETIEKYTKMLALELNVIGLLNIQYAI
                     VNDTVYILEANPRASRTVPLVSKVCGIPMAKIATKLMLGSKLSPDLTVKKKIKHFGVK
                     EAVFPFNMFPEVDPVLGPEMRSTGEVLGIANSFGVAYFKAQDAAGWRLPLEGNILITV
                     NRKDRESALEVAKKFHSLGFTIIATEGTSKYLDEHSIPTQMVFKLNEGRPDVVDRIKN
                     KEINLIINTPFGRISEVDDSYIRKAAIKYKIPYITTIAAARAAVRGIEMIKRYGEETP
                     KSLQQYHSEMS"
BASE COUNT         1254 a          682 c          823 g         1012 t
ORIGIN      
        1 atgcgttcgg ttggcgaggt catgagcata ggaaaaaatt ataaggaggc ttttcagaag
       61 gcaatacgtt ctttagagat tggccggcat ggacccggct ttgctaaaga ttttaataag
      121 cttccacttg aagaactgat acgcctgctc agtacaccat caagcgaacg acacttcatc
      181 atgtatgaag ccttgcgaaa aggtgccgat attgcaattt taaaagaacg cacacaaata
      241 aaagaatatt ttatacaaca aatgaaagag cttgttgatt tagaaaatga gatgcttcca
      301 tttgcaaaaa aatcacttcc cgactctctt ttaaaaactg caaaggagtg gggattttct
      361 gatagatacc tttcaaaaat acttgaactt cctgaagatg caatccgcaa acaaagaata
      421 tcgctgggag ttgttgagcg atgggaagct gtaccggtaa gcggtgtaga agatgctgct
      481 tattactatt ccacatacaa tgcacccgat acagtatcag tatcacatcg taaaaagata
      541 atggtgcttg gaggaggacc taacagaatt ggtcagggca ttgaatttga ttactgttgc
      601 gttcatgcag cttttgctat aaaagaagct ggatatgaag caatcattat caactgcaat
      661 cccgaaacgg tatccaccga ttatgatact gctgacaagc tgtattttga accactaaca
      721 gtagaagacg tactgagtat ttatgaaaaa gaaaaacccg aaggtgttat tgtacaattt
      781 ggcgggcaga ctccgcttaa tattgcaggt gaactggtca aagcaggggt aaaaatttta
      841 ggaacttctg tcgattctat tgatttagca gaagaccgtg atcgttttag aaaagtaatt
      901 acaaaattaa gcatcccgca gcctgaatcg gggatggcat catcgcttga agaagcgttg
      961 tcgattgcca accaaatagg ataccctctt atggtacgcc cttcatatgt actgggtggt
     1021 agggcaatgg aaataatata tgatgaagag atgcttaaac aatacgtaaa agctgcggta
     1081 gaagtaaccc ctgaaaggcc aattttgata gaccgttttc ttgttgatgc tatagagtgt
     1141 gaagctgatg ccatctgtga tggtgaaaat gtttttgtcc cttctgtgat ggaacatatt
     1201 gagcttgcag gtgttcattc cggagattca gcatgtgcta tccctcctga taacataaaa
     1261 aaagaacata ttgaaactat tgaaaaatat accaaaatgc ttgcacttga gcttaacgtt
     1321 atagggcttt taaatataca gtatgctatt gtaaatgata cagtatatat tcttgaagca
     1381 aatccaaggg catcacgtac ggtaccactt gtttcaaaag tatgtggcat accaatggca
     1441 aaaattgcta caaagttaat gcttggcagc aagctatctc ccgatttaac agttaaaaag
     1501 aagattaaac actttggtgt taaagaagca gttttcccat ttaatatgtt ccctgaagtt
     1561 gaccctgttt taggacccga gatgcgttct accggggagg tgcttggcat agccaatagc
     1621 tttggcgttg catattttaa agcacaggat gctgcaggat ggcgactgcc cttagagggc
     1681 aatatactca ttactgtaaa tcgcaaagat cgtgaaagcg ctcttgaagt tgcaaaaaaa
     1741 tttcattcac tgggatttac aataattgca actgaaggta catcaaaata tcttgatgaa
     1801 cacagtatac caacacagat ggtttttaaa ctcaacgagg gaagaccgga tgttgttgac
     1861 aggattaaaa acaaagaaat taacctcata atcaacacac catttggccg gataagtgaa
     1921 gtggatgatt catatatccg taaagctgca ataaaatata aaattccata tatcaccacc
     1981 attgcagcag caagagcagc agtcagaggt attgaaatga taaaacgcta tggtgaagaa
     2041 acaccaaaat cactacagca ataccattca gagatgtcgt aatttggtat aaaaatgatg
     2101 ttgagggtgc gatgtactcc cccttaaacg aaagggggaa ttatcaaggc tctgacccca
     2161 ttttataagt atcgattcta tccctatggg attagactgc aaaagcaatt atgaagaatt
     2221 aacagcatat tttatgctga taatgactta ttactgtata tgaacgaaaa aacagcgaag
     2281 gcacggatgc cgtagcgcca tacggctcgt gcgaggacaa agacaggatg tcttccgcag
     2341 cacgatggca aagacataat ccatacagcg ggataaggca caccgccgcc tgaggtgcgc
     2401 tttaggggtt ggtaagggga catttagcgc ataaatgtcc ccttacaaaa cgataaaact
     2461 tttattatca aacaaaagaa agtatgagaa agaaacatga atatatatct aacattcctt
     2521 ttaaaaaaag taatgcctgt cattctgagg cgcagacgaa gaaactcttc gttgtataaa
     2581 caaaaataat tcaccactgt cgtcaaaaca acaataaaca gccatacagg gatgtatggc
     2641 gcatttggat gcgggcgcat gagaggtcag cctgccgtag atgacactac atagccatgt
     2701 cattaaacca ggcgggaaag ctccgactcc attttttcat cactgcggga ttaacgaaaa
     2761 aacagcgaag gcacggatgc cgtagcgctc tacggctcgt gcgaggacaa agacaggatg
     2821 tcttccgcag cacgatggca aagatatact ataatacaga aaataaggca caccgccgtt
     2881 tgaggtgcgc tttgggggtt ggtaaggggg tatttagcgc ataaatatcc ccttacgaaa
     2941 ggataaaact tttactacca aacaaaagtt atacatgctc tgatcccatt ttttataaac
     3001 gaaagttttt ataaaacatt acacaaaagt aaaaaaaata gttgacaata gatgctatgt
     3061 actttaaaaa gcttctattg atgtaagata ttctttgaaa acagtatcat gatatacaac
     3121 tttgtatatc gccaacctgc taaaaatgta aaaagtggcg tttttggggg gaggttggcg
     3181 cacgtggaag tgctggaaaa tccagcagtt acaagtttgt aatacaaaat catggtaaaa
     3241 atagtgattt ttgcgatagg ttggcgaaaa tgggcttaaa aacgctggaa attccagagg
     3301 tttacagggt aagggtctga aaactgcccc gctgataggg gattgagact ttgagaagta
     3361 ccgtgcaaag tatggtacta ttttctttgt ctgaaaactg ccccgctgat aggggattga
     3421 gacatagctg tttcagattt gaattttgag tgcagcccct tttgtcgtct gaaaactgcc
     3481 ccgctgatag gggattgtga cggtatgatc gatgaagcga gcccaacagc ccaaccaaca
     3541 gccttccgtc tgaaaactgc cccgctgata ggggattgag acgtaaccgt taaaagttga
     3601 aagttataat gttgaaagtt gtggtgtgga ttgattgttt gctgttttga atgaagttgt
     3661 aagttttaaa tatgttttac taaaaataaa tataaaagct gacccctttt tttatctcat
     3721 ctctacggaa tgaaaccaca aaaactgtta tgaagaatta acagcatatt t
//
LOCUS       DAOSOX010000219         3765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24455_length_3765_cov_8.492453, whole genome shotgun sequence.
ACCESSION   DAOSOX010000219 DAOSOX010000000
VERSION     DAOSOX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24455_length_3765_cov_8.492453"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PLX22_11355"
     CDS             complement(<1..902)
                     /locus_tag="PLX22_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT20533.1"
                     /translation="MARILVVAEQRNGKISDATLELCKAAKVLASAMGASPAAAVFYK
                     DDSLAKEVAQYIPEVFSVVDAKLDVYNADTYTAAAKAVVESQDVKGVLIPHTYDGTDY
                     AAKLAMAIGCGIVTNCNKCEAQGGTVVFTRNTYNGKIQEQRSVKTDKFVATFEKGAFE
                     KEAAGGAGSVTAVTASIPDARRKFKQFVETMSGSVDITQAKVIVSGGRGTKEKDKYND
                     VIVNFAKKLGGEYAASRPVVDAGWTDAARQVGQSGKTVTPDLYIACGISGAIQHVAGM
                     KGSKVIVAINKDPEAPIFNIATYG"
     gene            complement(914..1702)
                     /locus_tag="PLX22_11360"
     CDS             complement(914..1702)
                     /locus_tag="PLX22_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT20534.1"
                     /translation="MKIAVLVKQVPDMESKFKIIGDSKIDESQIAFKMNDFDEYAVEA
                     ALQLKEKFSGEVIIISAGPERASKEIRQAFAMGGDWGIQIQDEQVDAGDNFVVASALC
                     KAVESLGGVDLVLTGVQAEDDQAAVTGVMIADMLGMPHCTNVVKIEVGGDGKSMTVNR
                     ELEGGLNEVLEMAMPAVLTIQSGINQPRYPTLPGIMKAKKKRLDVKKAADLGVAAVGE
                     AGSKTKFVKMFFPVSDHKAEIIEGDPKSAAAKLVDKLRNEAKVI"
     assembly_gap    1813..1912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1913..3690)
                     /locus_tag="PLX22_11365"
     CDS             complement(<1913..3690)
                     /locus_tag="PLX22_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20535.1"
                     /translation="MSRKNIIIALCILCFGFIVYGQEGIKLQQKNAIYHNKKGWESLQ
                     AGNAYSAIVSFQGALSQNPKYKESLLGLGYAYVQTQGYNESIKLFDKVLKLYPDNEQA
                     LMGMAKAYTGLGRYKEAMKLYDRLNELSYANNESRYGMAYLYYKMGKMLWAKRLCDRI
                     LQNDPYYYDALLLYADIKAKDKRVDDAIDFVKKAIDAKDDYPDAFVKLAEMLYLKYVY
                     SGDSAYLLQATDELQKALVMNKTHLQANRDMGFLLYVQGDYQNAITYYESIVKNYPYI
                     VNNEILYNVGLMYELTGNIMKALEYYTKALSKDNLNSMIAMKMRQLSLLEHIKIGHPV
                     RIQSSNDDFASSQELRKKGFPDLSMLFLRLSLALNPNNSTARQELLRIYETSGYFELY
                     YDEIKKMHSMNPTQKSQDLMSIAVFQRRDKLFFKLGYDKEEIPRDVPEVLVLDFTPDV
                     PVPAFADAGSVFANSINFAMGQFGRYQNFPMSQRLSIIKSLSSNNLDDILKNLNDKMN
                     NNEIPRISYLLYGEYNLSGRYIECNAKLMDAKTGVVIAHFTESDNSKYSTHTISIDLA
                     KKMYDTIPFRGKIISYSEDMAVINLGT"
BASE COUNT         1095 a          810 c          567 g         1193 t
ORIGIN      
        1 ataccatagg tagcaatatt aaaaatagga gcttctggat ctttgttaat agcaacaata
       61 accttagaac ccttcattcc ggcaacatgc tgtatagcac cggaaatacc gcatgcaata
      121 taaagatcag gagtaacagt ttttcctgac tgccccacct gccttgctgc atctgtccag
      181 cctgcatcaa caactggacg tgacgctgca tattcaccac ctaatttttt ggcaaagtta
      241 acgataacgt cattatattt atctttttcc tttgttccac gaccacctga tacaataacc
      301 tttgcctgag taatatcaac cgaacctgac atcgtttcaa caaattgttt aaattttcga
      361 cgcgcatcgg gaatagatgc agttacagca gtaaccgaac ctgcaccacc agcagcttct
      421 ttttcaaaag cacctttttc aaacgttgca acaaatttat cagtttttac tgaacgctgc
      481 tcttgaatct tcccgttgta ggtatttcgc gtaaaaacaa cagttccgcc ctgagcttca
      541 catttattac agttagttac aataccacat cctatcgcca tggctaattt tgcagcatag
      601 tcagttccat cataggtatg tggaattaaa acgcctttaa catcctgact ttcaacaaca
      661 gcttttgctg cagcagtata agtatctgca ttataaacat ccagctttgc atctactact
      721 gaaaatactt caggtatata ctgtgcaact tcttttgcta agctatcatc tttataaaaa
      781 actgcagcag ccggggatgc acccattgca gatgctaaaa ccttggcagc cttacaaagt
      841 tctaatgtag catcactgat tttaccgttt ctttgttctg caactactaa tatacgtgcc
      901 attgtgtacc cccttatatc acttttgctt catttcgtaa tttgtcaaca agcttagcag
      961 cagctgattt tggatcaccc tcgattatct cagccttatg atctgatacc gggaagaaca
     1021 tttttacaaa cttagtttta gaccctgctt caccaacagc agctactcct aaatcagcag
     1081 cctttttgac atccaatctt ttcttcttgg ctttcataat tccaggaagg gttgggtatc
     1141 ttggctgatt tataccagac tgtatggtaa gaacagccgg catagccatt tctaatactt
     1201 cattcaatcc accttcaagc tctctgttga cagtcattga tttgccatca ccgccaactt
     1261 caattttaac aacattggta caatgtggca tacccagcat atcggcaatc attactccag
     1321 taactgccgc ctggtcatct tcggcctgaa caccggttaa tacaaggtca actccaccta
     1381 agctctctac agccttgcaa agcgctgatg caacaacaaa attatcacct gcatcaacct
     1441 gttcatcctg aatctggatg ccccagtcgc cacccattgc aaaagcctga cgaatttcct
     1501 ttgatgctcg ctccggccct gctgaaataa taataacctc accagaaaac ttttccttta
     1561 attgcagcgc agcctcaaca gcatactcat caaaatcatt cattttaaaa gcaatctggg
     1621 attcgtcgat cttactgtcg ccaataattt taaattttga ttccatatct ggaacctgct
     1681 taactaagac agctatcttc atagtcccac tccctttttt aaaattttat gcaaaaaaaa
     1741 tagaataagc ccatttgtgc acaatggtat aaattgcttt ttatatgtca agatttatta
     1801 ttaaattaca atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngttcctaa
     1921 gtttatgact gccatatctt ccgaatatga tataattttt cccctgaatg gtattgtatc
     1981 atacattttt tttgccagat caatcgaaat ggtatgagtt gaatacttac tattatcgga
     2041 ttcagtaaaa tgagctatca ccacaccggt ctttgcgtcc atcagtttag cattacattc
     2101 aatatatctt cctgaaagat tatactcacc gtatagtaaa tacgaaatcc ggggaatttc
     2161 attgttgttc attttatcat taagattttt gagaatgtca tccaaattgt ttgacgataa
     2221 gcttttaatg atagataacc tttgagacat ggggaaattt tgataacgtc caaattgacc
     2281 cattgcaaag ttaatagaat ttgcaaagac gctgccagca tctgcaaaag caggaacagg
     2341 aacatcagga gtaaaatcaa gcaccaatac ctcaggaaca tcacggggaa tctcctcttt
     2401 atcatatccc agcttaaaaa aaagtttatc cctgcgctgg aatactgcga tgctcattaa
     2461 gtcctgcgat ttttgagttg gattcattga atgcattttc tttatttcat catagtataa
     2521 ttcaaaataa cctgaggttt catatatacg caatagttcc tgacgtgctg tactattgtt
     2581 gggatttaat gctaatgata atcgcaaaaa tagcatggat aaatcgggaa agcctttttt
     2641 acgtaattcc tgcgatgagg caaagtcatc gttgctactt tgaatccgaa caggatggcc
     2701 aatcttaata tgttcaagca agcttaattg ccgcatcttc atggctatca tactattaag
     2761 attatctttt gaaagagctt ttgtataata ttccagagct ttcataatgt ttccggtaag
     2821 ctcgtacata agccctacat tatacaatat ttcattattg actatatagg gatagttttt
     2881 aacaattgac tcatagtagg taatagcatt ctggtaatct ccctgcacat acaacaaaaa
     2941 acccatatcg cggtttgcct gtagatgtgt tttgttcata acaagggctt tttgtaattc
     3001 atcggtagct tgtaataaat aggcactatc gccagaataa acatatttta ggtataacat
     3061 ctcagctaac ttaacaaaag catcaggata atcatccttt gcatctattg cctttttaac
     3121 aaaatctatt gcatcatcaa cacgtttatc tttagcctta atatcagcat ataatagcaa
     3181 tgcatcataa taatagggat cattttgtaa aattctgtca cacaaccgtt ttgcccacag
     3241 cattttcccc attttatagt acagatacgc cataccgtaa cgcgactcat tgtttgcgta
     3301 cgacagctca tttaatctat catataattt catcgcttct ttatatctgc caagtccggt
     3361 atatgctttt gccattccca taagcgcctg ctcattatcc ggatagagct tcaatacttt
     3421 atcaaacaac tttattgatt cattgtatcc ctgagtttgc acataggcat accccaatcc
     3481 taataaggat tctttatatt tgggattttg cgacagtgca ccttgaaaag atacaattgc
     3541 tgaataggca ttgcccgcct gcaggctttc ccatcctttc ttgttgtgat atatggcatt
     3601 tttttgttga agtttgatac cttcctgtcc atacactata aacccaaaac atagtataca
     3661 tagcgctatt atgatatttt ttctgctcat gtgttttttt attgataaaa taatagtatt
     3721 gaaaatcaaa gtaattaaac agctacctta caatttaagt caaca
//
LOCUS       DAOSOX010000220         3704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_24950_length_3704_cov_8.289942, whole genome shotgun sequence.
ACCESSION   DAOSOX010000220 DAOSOX010000000
VERSION     DAOSOX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24950_length_3704_cov_8.289942"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLX22_11370"
     CDS             <1..269
                     /locus_tag="PLX22_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20536.1"
                     /translation="PHFYSLKTSRFAQFSELTPMIHMLQGKIMKTNMHENDPYLIPPL
                     WYMVSIIESSSENKVDKFLLRCDSSSKLRAVLDEISFFYGRHGY"
     gene            275..1057
                     /locus_tag="PLX22_11375"
     CDS             275..1057
                     /locus_tag="PLX22_11375"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOT20537.1"
                     /translation="MIDYHVHTYLCDHASGTPGDYIEKALEARLFEIGFSDHAPLPEH
                     LRNDITMEPEETEMYCSMLEDVKDKYPIAVKIGFEVDYPLHPTFDTHYFHDNRIDYMI
                     GSCHFIDGWAFDHPLFVDEYNKRDINQVYRDYYSIVFDMVNSGLFDIIGHFDLVKKFG
                     YRATVDLTDHILPIVKLAGKQATAIEINTSGLRKPVKEIYPSYDIIKLMFENNVPITL
                     GSDAHAPEEVAYNFYDALLLIKRAGYKKIAGYSKRKQYSIIL"
     gene            1054..1776
                     /locus_tag="PLX22_11380"
     CDS             1054..1776
                     /locus_tag="PLX22_11380"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOT20538.1"
                     /translation="MITLKKDTNNIYRVYGLCNLQAGVIAKAVNTIDYSDTDNNVRFK
                     EKLLIYKATGILPEHIVMLDQVHGSSIIEMNYRPQKNDPFVTQADGFVTMVHGLCLVI
                     RTADCVPVILYDSAKGILAALHSGWRGCMQNITAEGIKKLYQKGSSPASVHVMILPSI
                     SRDAYEVGDDVASQFNGYILNKNGRCYLDMWNHIADTAMKNKIPCNNIYVTGICTFQN
                     NTEFFSYRKGDKGRNLNFVYLS"
     gene            1982..2896
                     /locus_tag="PLX22_11385"
     CDS             1982..2896
                     /locus_tag="PLX22_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20539.1"
                     /translation="MEKLQLSKLLDTHNHNKTLDEIKSLYRKYYSEFAIIEDLFFIVK
                     DLFYGEFPGYQKCNTQYHDFQHTVDATLAAMRLLDGYNIKCKIIPEDIAYCLIIAALL
                     HDTGYIQEDWDTEGTGAKYTAVHVGRSVEFLRKHYTKFQIAEKYIPIISLCIQSTGLS
                     TNFKELAFISKEHMIAGAMMGTADLLGQMSDREYLEKLLFLYYEFKEAGIPGYSTEYD
                     IIWKTIDFYVMTVKRMKRDYLNVQRYCTYHFEKRYAIPSNLYMVAINRNIRYIKKIIA
                     DESTNFRHKLKRGDQYQLQMLSQQSVHH"
     gene            3131..3658
                     /locus_tag="PLX22_11390"
     CDS             3131..3658
                     /locus_tag="PLX22_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20540.1"
                     /translation="MKKAISTIVMLKLVLLAANVWAYSVVTIQKWEINQNDVIKQYLP
                     NANYKQYTLNQIAGYNNKLLNYILAIDADYKNKITIFRTGKGIARDFMFIGTKLYVVT
                     DYMQLSNAESSSEVISALGKIYGTPQKQTEANTTTYTYTKDQTKIIAILEAKGQKYSM
                     TIYYYYKNLFKSLLQ"
BASE COUNT         1272 a          581 c          705 g         1146 t
ORIGIN      
        1 atcctcattt ctattcatta aagacatcac ggtttgccca gttttccgaa ttaacaccca
       61 tgatacatat gctgcagggt aaaataatga aaacaaacat gcatgaaaat gatccctatc
      121 ttataccacc attgtggtat atggtatcaa ttattgaatc atcatctgaa aataaagttg
      181 ataaattttt gttgcggtgc gatagttctt cgaaattgcg ggcagtactg gatgagatat
      241 ccttcttcta cgggcgtcat ggatattaaa caccatgata gattaccatg tacataccta
      301 tctgtgcgat cacgcatcag gtacgcccgg tgattatatt gaaaaagcgt tggaagccag
      361 gctctttgaa attggttttt ctgatcatgc accgttgcct gaacatttgc ggaatgatat
      421 taccatggaa cctgaagaaa ccgagatgta ctgctcaatg cttgaagatg taaaggataa
      481 atatcccata gcagtgaaaa taggttttga ggttgactat ccgcttcacc caacatttga
      541 tacccattat tttcatgata atcgcattga ctatatgatt ggttcatgtc attttattga
      601 tggctgggct tttgatcatc ccctgtttgt agatgaatac aataaacggg atatcaatca
      661 ggtttatcgt gattactata gtattgtttt tgacatggtc aattcagggc tttttgatat
      721 aattggccat tttgatcttg taaaaaagtt tggttacagg gcaactgttg atctaaccga
      781 tcatattctt cccattgtaa agcttgcagg aaaacaggca acagccattg aaataaatac
      841 gtcagggtta cgcaaaccgg tgaaggaaat ttatccgtcg tatgacatta ttaagcttat
      901 gtttgaaaac aatgttccca ttacactggg ttcagatgct catgcacctg aggaggttgc
      961 ctataatttt tatgatgccc tgcttttgat aaagcgggcg gggtacaaaa aaattgcagg
     1021 atacagtaaa cgaaagcaat attcgataat actatgataa cattaaagaa ggacacaaat
     1081 aatatctaca gggtttatgg actgtgcaat ttgcaggcag gtgttatcgc aaaagctgtc
     1141 aatactatag attattctga tactgacaat aacgtccgct ttaaagagaa attacttatc
     1201 tataaagcta cagggatatt gcccgaacat attgttatgc ttgaccaggt tcatggcagc
     1261 agtatcattg agatgaacta tcgcccacaa aagaatgacc cctttgttac acaagctgat
     1321 ggttttgtta ccatggtaca tggattgtgc cttgttatac gaacggctga ttgcgttccg
     1381 gttatactgt atgattcggc aaaaggtatt ctggcagcgt tgcattccgg ctggcgcgga
     1441 tgtatgcaaa atattactgc tgaagggata aaaaaattat atcaaaaagg ttcttcacct
     1501 gcttcagtcc atgttatgat actgccgtca ataagccgtg atgcgtatga ggttggggat
     1561 gatgttgctt cccaatttaa cgggtatata cttaacaaaa atggcaggtg ctatttagat
     1621 atgtggaatc atattgctga caccgcaatg aaaaacaaaa ttccgtgcaa caatatatat
     1681 gtaacgggga tatgcacgtt ccaaaacaat acagaatttt tttcatacag gaaaggagat
     1741 aaaggtagaa atttaaattt tgtatattta tcatagcaat agttaatttt tagcctgtga
     1801 aaataaatat aaacctgcat tgtaataaat acaatgccaa tttattcaga gattgtgata
     1861 tgggatattg ctttttacaa gagcatttat atatggttaa tttttttaaa aaaactgaat
     1921 aaaagcttgc attttttaaa aaaaataaaa tattatatta ataagtagtc aggaggaaag
     1981 tatggaaaag cttcaactgt caaaacttct tgatacgcat aatcacaata aaacattaga
     2041 tgaaataaag tcattatata gaaaatatta tagtgaattt gcaataattg aagatctttt
     2101 ttttattgtt aaagatttat tttatggcga atttcctggt taccagaaat gtaatactca
     2161 atatcatgat tttcagcata ccgttgatgc aacattagca gcaatgcggc tgttagatgg
     2221 atataatatt aaatgtaaga taataccaga agatattgca tattgcctta ttattgctgc
     2281 tcttttacat gataccgggt acatccagga agattgggat accgaaggaa caggagcaaa
     2341 atatactgcg gtacatgtag ggcgcagtgt tgaattttta cggaaacact atacaaaatt
     2401 tcaaatagct gaaaagtata tccctataat ttcattgtgt atacagtcaa cagggctgtc
     2461 cacaaatttt aaggagcttg catttatatc aaaggaacac atgatcgccg gggcgatgat
     2521 gggaactgcc gaccttttag ggcagatgtc agacagagaa tatttggaaa agctgctatt
     2581 tttatactat gaatttaaag aggcaggtat tcctggttat tccaccgagt atgatatcat
     2641 atggaaaact atagattttt atgtcatgac agtaaagcgc atgaagagag attatctcaa
     2701 tgtgcagcgt tattgtacat atcattttga aaagcgatat gctattccca gtaatcttta
     2761 tatggtagca ataaaccgca atattagata tatcaaaaag ataattgctg atgaaagcac
     2821 aaactttaga cataaattga aacgtggtga ccagtatcag cttcagatgc tttcacaaca
     2881 atcggtacat cattaatatt ttaatgtgct tatgcaatgc aaatggcagg atacgtgtgt
     2941 tgtattatca atgtcctcgg ataaaaagat aaaagaaccc gtagaaattg cgatgatata
     3001 taagccatag taagggctta tttgatatac cacctgctac cagagcataa tggaaagaaa
     3061 aatgtttgac ataatatgtt catctgggtt attttttaac actatagtga taaagcaaaa
     3121 aggaatagat atgaaaaaag caataagcac aattgttatg ctgaaacttg tcttgctggc
     3181 ggcaaatgtt tgggcttata gtgtggttac catacagaag tgggaaatta atcaaaatga
     3241 tgttataaaa caatatctgc caaacgcgaa ttataaacag tatactctta atcagattgc
     3301 tggatataat aataaacttt taaattatat acttgccatt gatgcagatt ataaaaataa
     3361 gatcacgatt tttagaacag gcaagggtat tgccagagat tttatgttca taggaacaaa
     3421 attgtatgtt gtaactgact atatgcaact atccaatgct gaatcttctt ctgaagtaat
     3481 ctcagcgctg ggaaaaatat atggaacacc acagaaacaa acagaagcaa acacaaccac
     3541 ctatacctat acaaaagatc aaacaaaaat tattgcgatt ttagaagcta aagggcaaaa
     3601 atattcaatg actatatatt attactataa aaatttattt aaaagcctgc tgcagtaagt
     3661 agtaattgtg ggttttcatg taacaatgtc tatggcgata tttt
//
LOCUS       DAOSOX010000221         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_25390_length_3655_cov_9.745000, whole genome shotgun sequence.
ACCESSION   DAOSOX010000221 DAOSOX010000000
VERSION     DAOSOX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25390_length_3655_cov_9.745000"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLX22_11395"
     CDS             complement(<1..595)
                     /locus_tag="PLX22_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOT20541.1"
                     /translation="MSTTIHALTIQRYANNGFGIGFLHNKAVFIPYCAIGDVLTVEII
                     NSTKTYSFARIVDIVNPSPIRIQPRCPAFACCGGCDFLHIPYEEELSLKKNLFINTLQ
                     RIGGIPIQSVPGIEIIASQRFAYRSHATFQSNGADIGFFKKDSHVIQPLPQTGCLLVH
                     NDINRAIVTGKKNSGAIRIAVDHYNKIGSDPNDVITEI"
     gene            874..1470
                     /locus_tag="PLX22_11400"
     CDS             874..1470
                     /locus_tag="PLX22_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20542.1"
                     /translation="MARKLRVQLPEHTYHIMSRCIEWRDMMQEEYFKACFVDILNRTK
                     QKYDFKLIAYCIMDNHVHIVIHTTSTGAPIARIVQYIKARFAEMYNKITGRTGPFWNE
                     RYKDIIVQFAHDAFHYLLWLLWYIAFNPVRKRQCDSPVKYRYSSIQSYVQEGADVGVP
                     IDHHEYFLQLGDSFSERVKRFLQYEEAYRKRYSIAQWV"
     gene            complement(1585..3282)
                     /locus_tag="PLX22_11405"
     CDS             complement(1585..3282)
                     /locus_tag="PLX22_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT20543.1"
                     /translation="MILLNPKKYSAQFSDKESEEIMRKTIDFFETKGLKKILDDDHNY
                     VWYDDFLQFQKDNQIFAKFFTPEKYGDKNSRWDTYRIVNYAEILGFYGLCYWYTFQVS
                     ALGLGPIWIGKNEEVKQKAAKLLKEGAIFAFGLSEKEHGADIYSSDMMLYPNGDGTYR
                     ANGDKYYIGNANKAAMVSTFGKFADTGDYVFFVADSQHEKFECIKNVIQNQSYVAEYA
                     LHDYPIAEADILDKGRDAWDSSLNTINICKFNLGWGSIGIATHCFYEALNHAGHRNLY
                     GKYVTDFPHIKQFFVDAYTRLVAMKLFALRATDYMRCANENDKRYLLYNPMVKMKVTT
                     QGEEVINLLWDIIAAKGFEKDTYFHMAARDIRALPKLEGTVHVNMALIIKFMFNYFFN
                     PKEFPEVPQRLDAANDDFLFNQGPTKGLSKIQFHDYNIAYNSVNLPNVEIFKKQINAF
                     KDFMTGATPDKQQSEDIDFLLSIGELFTLVAYGQLILENKTIYAVEDDLIDQIFDFMI
                     RDFSKFALQIYSKTSSTSKQQELCLKMIEKPVVDHDRYNRVLEKYVYALKDEYYMNE"
BASE COUNT         1102 a          768 c          692 g         1093 t
ORIGIN      
        1 caatctcggt gatgacatca ttggggtcag agcctatttt attatagtga tcaacagcaa
       61 tcctgatggc accggaattt tttttgccag taactatcgc cctattaatg tcattgtgta
      121 cgagcaggca gcccgtttgt ggtaatggct gtatgacatg agagtctttt ttaaaaaatc
      181 caatatccgc tccgtttgat tgaaatgttg cgtggcttcg gtaggcaaaa cgttgcgagg
      241 caatgatttc tataccgggg acgctctgta tgggtatccc accaattctt tgcagtgtgt
      301 ttataaaaag atttttttta agcgatagtt cctcttcata gggaatgtgt aaaaaatcac
      361 aacctccgca gcaggcaaac gcagggcagc ggggttggat acgaattggc gaagggttaa
      421 caatatcaac aatcctggca aatgaatagg tttttgtact gtttataatt tcaacagtaa
      481 ggacatcgcc aatggcgcag tatggtataa agacggcttt attgtgtaaa aaaccaatac
      541 caaaaccgtt attggcataa cgctgaatag tgagtgcgtg aattgttgtg ctcatatata
      601 gatactggat aactattgat ggtaaaaaga tatcatgcta tacctatgca attgtccaat
      661 gatgattaca tagtataagg atactgttgg aagttaggga tcaatattca attaaaattt
      721 gtgaatatat gctctgaccc cttgtagata gtgttttttc aaaaaaaatc gcaaaaagta
      781 tcaacaaata caccaattct ttttaatatt ttctatctcc ggcgttatgt atagtaggga
      841 tgtacattat aataaaaaca cggaggaata cccatggcac ggaaattgcg cgtacaactg
      901 cccgagcaca cctaccacat catgagccgc tgtatcgagt ggcgtgatat gatgcaggag
      961 gagtacttta aagcctgttt tgtcgacata ttgaacagaa caaaacaaaa atatgacttt
     1021 aagctgatag cgtactgcat catggacaat cacgtccata ttgtcatcca cacaaccagc
     1081 acaggagcgc ccatagcacg cattgtacag tatatcaagg cacggtttgc cgagatgtat
     1141 aataaaataa ccggcaggac cgggccattc tggaatgagc gctataagga tatcattgta
     1201 cagtttgccc atgatgcgtt tcattatctg ttgtggttat tgtggtatat agcgttcaat
     1261 ccagtgcgga agcgccagtg cgatagtcct gtcaagtatc gctacagcag catacaatcc
     1321 tatgtacagg agggtgctga tgttggtgta ccgatagacc atcatgagta ctttctgcag
     1381 ttgggggaca gtttcagcga acgagtgaag cggtttttgc agtatgagga ggcgtaccgc
     1441 aagcgttatt ctattgcaca gtgggtataa aaaaggggtc agagcctatt ttaacgaata
     1501 ccaaaaaaga ggccgtcttt attagagaca gccccacttt tacgctttaa cacccgtata
     1561 caacaaattc aattactgta ttatttactc attcatataa tattcatcct tgagagcata
     1621 cacatatttc tccagaacgc ggttatacct atcgtggtca acaacaggct tctcaatcat
     1681 cttcaagcac aattcctgtt gttttgatgt actgcttgtt tttgaataaa tttgcagagc
     1741 aaactttgaa aagtcacgga tcataaagtc aaatatctga tcaatcaaat catcttccac
     1801 cgcatagatt gtcttgtttt caaggataag ctgaccatag gcaaccagcg taaacaactc
     1861 gcctatagac aataaaaagt caatatcctc actctgctgt ttatcagggg tagcaccggt
     1921 cataaaatcc ttaaacgcat taatctgttt tttgaaaatt tctacgtttg gaaggttcac
     1981 actgttatag gcaatattgt aatcatgaaa ttgaatctta ctcaacccct tcgttggacc
     2041 ctgattaaat agaaaatcat cattggcagc atcaagacgc tgaggaactt cagggaactc
     2101 ttttggatta aagaaatagt tgaacatgaa tttaattatc aacgccatat tcacatggac
     2161 agttccctcc agctttggca atgccctgat atcgcgtgca gccatgtgga agtatgtatc
     2221 cttttcaaaa ccttttgcag ctatgatatc ccacagtaaa ttgatgacct cctcgccctg
     2281 tgtggtcact ttcatcttca ccattggatt gtacaaaagg tatcgtttgt cattttcatt
     2341 ggcacagcgc atataatcgg ttgcacgcag agcaaaaagt ttcattgcaa caagccgtgt
     2401 ataggcatca acaaaaaact gtttaatatg cggaaagtcg gttacatatt tgccatacag
     2461 attacggtgt ccggcatgat tcaacgcttc ataaaagcag tgtgttgcaa taccaattga
     2521 accccatcct agattaaact tacaaatatt gatagtgttc agcgatgaat cccatgcatc
     2581 acgtccctta tcaaggatat cagcttcggc aattggataa tcgtgcagtg catattcagc
     2641 aacgtatgat tgattctgaa taacattttt tatacactca aatttttcat gctgcgaatc
     2701 cgcaacaaag aaaacataat caccggtatc agcaaattta ccaaatgttg acaccattgc
     2761 agctttattg gcattcccta tatagtattt atcaccatta gcccggtaag tgccatcacc
     2821 attaggatac agcatcatat cgctggaata gatatcagcg ccatgttcct tttcagaaag
     2881 cccaaaagca aagatagcgc cctctttcaa aagtttggca gccttttgct tcacctcttc
     2941 gtttttgcca atccatatag gtcccagacc caatgctgaa acctgaaagg tataccagta
     3001 gcaaagtccg taaaatccta aaatttctgc atagttaaca atcctgtagg tatcccatcg
     3061 gctgttttta tcgccatatt tttctggagt aaagaacttt gcaaatatct ggttatcctt
     3121 ttgaaattgt aaaaagtcat cataccacac atagttgtga tcgtcatcca gaatcttttt
     3181 taaccccttg gtttcaaaaa aatcaattgt tttgcgcata atctcttctg actctttgtc
     3241 agaaaactgc gcactgtact tctttggatt taacagtatc ataccccacc tctattcaaa
     3301 ttatagttac aaatttgaat atatcatgca cctgatgcat tgtacagaaa aaattacacc
     3361 gttatgtatt tttttaaaca acttatttca taaattgttt acctgtgttt tgggcgtgcc
     3421 cctgccaaca gtcaaaatca tcataccaac ggaacaccac ctctattccc tctatgcata
     3481 atcccgctga tattatggca gggtcgggct actgcaggct acgctatcgc ttcggccacg
     3541 ggcatgtata gtataaaagc tttcacatta tctatcaata ccaatccgta atgcctatgt
     3601 gtctctacaa ataaacgccc gtggctgctt ttcaagcacc ttgcgtatcc ctcac
//
LOCUS       DAOSOX010000222         3622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_25728_length_3622_cov_8.562097, whole genome shotgun sequence.
ACCESSION   DAOSOX010000222 DAOSOX010000000
VERSION     DAOSOX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25728_length_3622_cov_8.562097"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..797
                     /locus_tag="PLX22_11410"
     CDS             24..797
                     /locus_tag="PLX22_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20544.1"
                     /translation="MIARQYLITPAAGKRMIAQSMLYIPEIIEALRSKTIVIVAGTTN
                     GYIAEEMLRHVNQSEGFSMQHFFRGITLPSWYKRTESGRLPDESTFKGDVVIKGGIYE
                     KNKTIFDVMGDLQKGDIIIKGANAVNIELQQAAVLIGHPQAGTTGAIMQVVIGKRVQL
                     YIPVGLEKRLHDNINELSQMINATTTEGVRYFPVTGNIITELDAIHLVIGAQAKLFAA
                     GGVGGAEGSYWVLVRGSKEQIQKMDEWYATVKNEPNFVI"
     gene            complement(822..2996)
                     /locus_tag="PLX22_11415"
     CDS             complement(822..2996)
                     /locus_tag="PLX22_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20545.1"
                     /translation="MKKKVLFVILAAFFIGFSSSIAQAIMIRELLTIFRGNELSVGII
                     MAIWLGGIFTGAVFSKTKQIVHPKFIIAMIIVHPFLFLVSLIVLYSIPIVYSSYGAFY
                     QLDAEIMIALLCLFPQCFVIGYVFPVLIQYADELLIKHPGSIVFLLESSGAFAGGIVF
                     TFFILFVLNPIGTAIVLLLCATILSLLIYNQKKHYVTITLVCIILLFLTTQTNKAEQA
                     LIQKSFAINHPGSLLLYKRTPYQMMYVAKNYDTYSLYGNNIFYTQLPDDYEVRPLLHA
                     LFASIHDILGKNILIAGDTLYLPYVATKLQASIDYCTVDRSLWNTFHTLGKQLYPNFD
                     FNKLNVIFSDTRGLLSKTPRRWDGIVIIPPPPDSILHNRLYTLEFFQFCRMHLNQNGV
                     FIMQIEGFANIMNPSLQRYISSVLNSFTKMFPTSIVSAGETIYLIGFNTGNTIDFNNY
                     RQNYLANFLKSDTGKSVAALIPHFNPKELDMFFQSTHLAYLNTAIKHSAMLNTDIMPQ
                     AYFEYLANTLKQTDSYTGNFIMQPAVMAIIMICIVVAATVYFLRRGKEHAVKGLVVFI
                     AGFISMSAMVIALMAYQNVYGILYYRIAFCNALFMLGLASGCTTTFAAKPLYAMRIVI
                     LIGSLAMLALFIIMKHVWLLYCAIFFIAAITGSLLPLLLKNTQETYTKRLLASTLDAS
                     DHLGAMCGALAAPFIVLPIFGMYSIGILLLLSISLFIFKKWI"
     gene            complement(2993..3376)
                     /locus_tag="PLX22_11420"
     CDS             complement(2993..3376)
                     /locus_tag="PLX22_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20546.1"
                     /translation="MWIELVNLVIPTLNDNPNDIKAMARFIKSHVGDTTPLHFTRFYP
                     IYQLKNIPPTPVSTLERCKEIAVSEGLKYVYIGNVPGHPANNTYCHACGKLVIERSGF
                     YTTSNKLKSGHCPHCNTRIPGIWTS"
BASE COUNT         1186 a          622 c          755 g         1059 t
ORIGIN      
        1 atattcttga tagtgaggta actatgattg ccagacaata ccttattaca cctgctgctg
       61 gaaaaaggat gattgcacaa tcaatgctgt atattccaga gattatagaa gccctgcgca
      121 gcaaaactat tgttatcgta gccggtacaa ccaatggata tattgctgaa gagatgctgc
      181 gccatgtcaa tcagtcagaa ggattttcaa tgcaacattt ttttagagga ataacattgc
      241 cgtcatggta taagcgcact gaatcagggc gattgcccga tgaatcgaca tttaaaggtg
      301 atgtcgtcat taaaggtggt atatatgaaa aaaataaaac aatatttgat gttatgggtg
      361 acctgcaaaa aggtgacata atcatcaaag gagcaaatgc agtcaatata gagctgcagc
      421 aggctgcagt gttaataggg catccccagg ctggtactac cggtgcaatt atgcaggttg
      481 tcatagggaa aagagtacag ctttatattc cggtaggatt ggaaaagcga ctgcatgata
      541 atataaacga gttgtctcaa atgattaatg ccactacaac cgagggtgta cggtattttc
      601 ctgttacagg gaatattatc acagagcttg atgccataca tctggttatt ggcgcacagg
      661 ctaaactttt tgcagcaggt ggagtaggcg gtgccgaggg cagttactgg gttttggttc
      721 gtggaagcaa agaacagata cagaagatgg atgaatggta tgctactgta aaaaatgagc
      781 caaattttgt aatataatag ttgatgatgt atacaatttt tctatatcca ttttttgaat
      841 ataaacaggc ttattgaaag cagtagtaag atgccaatgg agtacatccc aaaaataggg
      901 agtacaataa acggtgcagc taaagctccg cacattgcac caaggtgatc actggcatcc
      961 agtgtagaag ccagcaatcg ttttgtatat gtttcctgcg tattttttaa cagtaatggt
     1021 agcaaagagc ctgtgattgc tgctatgaaa aaaatagcac aatacagcaa ccatacatgc
     1081 ttcatgatga tgaaaagagc aagcattgcc aaactaccga tgagtatcac tatccgcatt
     1141 gcatatagtg gctttgcagc aaatgttgtt gtacaaccag aagccaaacc aagcataaag
     1201 agcgcattac aaaatgctat cctgtaataa agaattccgt atacattctg gtaagccata
     1261 agtgcaataa ccatagccga catactgata aaacctgcaa taaataccac cagccctttt
     1321 actgcgtgct ctttaccccg tcgtaaaaaa tagacagtag cagcaaccac aatacaaatc
     1381 attatgattg ccataactgc tggttgcatt atgaaattgc ctgtatatga atcagtttgc
     1441 ttaagcgtgt tagctaaata ttcaaaatat gcttgcggca taatatcggt atttaacatt
     1501 gcagagtgtt tgattgcagt gttaagatag gcaaggtgag ttgactgaaa aaacatatcc
     1561 agttcttttg gattaaaatg agggattagt gctgcaacgg attttccggt atcagatttt
     1621 aaaaaattgg caaggtaatt ctggcgatag ttattgaaat caatggtatt gccggtatta
     1681 aacccaatta aatagatggt ttcgccggct gaaactatgg atgttgggaa catttttgta
     1741 aagctgttca aaactgatga gatatagcgt tgcaacgaag gattcataat attggcaaac
     1801 ccctctatct gcatgatgaa tacaccattc tggttaagat gcattctgca aaattgaaaa
     1861 aattctaatg tatataatct gttgtgaagt atgctatcgg gaggtggagg gataatgaca
     1921 attccatccc atcttcttgg tgtttttgac agcaagccac gtgtgtcact gaatataaca
     1981 ttaagtttgt taaaatcaaa gtttggatat agttgtttgc caagtgtatg gaaggtgttc
     2041 cataatgatc tgtcaacggt acaataatca atcgatgcct gtaattttgt agcaacatag
     2101 ggaagataca atgtatcgcc ggcaataagt atatttttgc ccaaaatatc atgtattgaa
     2161 gcaaacagtg catgcaataa tggccttacc tcataatcat caggaagctg tgtatagaaa
     2221 atattgttgc catagagtga gtacgtatca taattttttg caacatacat catctgatat
     2281 ggtgttcttt tatagagcag caatgaaccc gggtgattta ttgcaaatga tttttgaatc
     2341 agggcttgct ctgctttatt tgtttgagtt gttaaaaaca aaagaataat acaaaccagt
     2401 gttattgtga catagtgttt tttttgatta tatataagta gtgacagtat ggtggcgcag
     2461 agcagcagta ctatagctgt cccaatggga tttaaaacaa acagtataaa aaatgtaaat
     2521 acaatgccac cagcaaatgc acctgatgat tccagcaaaa agacaatgct tcccggatgt
     2581 tttatcaata actcgtctgc atactgaatc aatacaggaa aaacatagcc aatgacaaaa
     2641 cattgcggga aaaggcatag caaagcaatc atgatctcag catcaagctg gtaaaaggca
     2701 ccgtatgagc tgtatacaat cggtatgcta tatagtacaa ttaatgaaac taaaaacaaa
     2761 aaaggatgaa caataatcat tgctatgatg aattttggat gaacaatctg ttttgtttta
     2821 ctaaatactg caccggtgaa tattccgcca agccatattg ccatgataat cccaaccgac
     2881 agctcgttgc cccggaatat cgtcaaaagt tcccgtatca tgattgcctg cgctatgctt
     2941 gaagaaaagc ctataaagaa tgctgccaga attacaaata gcaccttttt tttcatgatg
     3001 tccatattcc cggtattctg gtattgcaat gcggacagtg tcctgatttg agcttattgg
     3061 aggtagtgta aaatccgctg cgttctataa caagttttcc acatgcatgg cagtaggtgt
     3121 tgttagcagg gtgtccgggc acattgccaa tatatacata tttaagtcct tcggaaactg
     3181 caatttcttt gcagcgctca agtgttgaaa caggtgttgg tgggatattt tttaattggt
     3241 aaatgggata aaagcgtgta aagtgtaatg gtgttgtatc gccaacatgg gattttataa
     3301 aacgtgccat agccttaatg tcattgggat tatcattgag cgttgggatt actaaattaa
     3361 caagttcaat ccacacattt ttcttttttg ccagtataat gttgtttaaa accggctgta
     3421 aatttccgcc gcaaatttcc aggtaaaatt tttgtgaata tgccttaaaa tcaattatgg
     3481 attcaagtca caagtgcaac ctttcttaaa aaagaagggt tgactgccaa taagtttact
     3541 cttcttttgc tagttaacac accaaacaaa agaggagata caaatggctt atcagcagtt
     3601 aacccttgaa gagagaagca tg
//
LOCUS       DAOSOX010000223         3620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_25747_length_3620_cov_8.232258, whole genome shotgun sequence.
ACCESSION   DAOSOX010000223 DAOSOX010000000
VERSION     DAOSOX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25747_length_3620_cov_8.232258"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PLX22_11425"
     CDS             complement(<1..155)
                     /locus_tag="PLX22_11425"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HOT20547.1"
                     /translation="MTNSTTIWNKVLGSVEAYINKQSFNMWFKNTEPVAFNEDVLVIK
                     VVDDVAKR"
     gene            498..755
                     /locus_tag="PLX22_11430"
     CDS             498..755
                     /locus_tag="PLX22_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOT20548.1"
                     /translation="MSVIIKDIQRNNTEIIRVEISEFKGKELINLRIWYQSIENGAVV
                     YKPTQKGVALSVDKFHELQEAVSKIAEYLHDKKFGTTPENV"
     gene            complement(736..1854)
                     /locus_tag="PLX22_11435"
     CDS             complement(736..1854)
                     /locus_tag="PLX22_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20549.1"
                     /translation="MPNIEIKASIRDTDDRNSNNTFSIYCENDFFKLFKINMDTSHRP
                     VNTYELESQFLHFFKDPYTLFPSLPFILEVMSDDEFQKLIYYLLNEKLLTPYHLFLIS
                     RAYPQYSLKIKHNISRHTISDIINVGKSIHKITYRDVLEAIYAFEELLFLKLRSKPYF
                     HYGTFIEQVHSILHHMRMVTLFQQKSFDEWVYEFIKSNLLYAVLAKCDDITIAAAFYN
                     NRHLISLLDNYISSKRINGIASLFEMECDTNLIISSQHRMIELFLNTISSHNKLYSQP
                     FNILLKKYMTPENMYYVLFEIGWFVIATALKQSSKKLCLECIKHFPEGAQYCIIDVYN
                     GVLNPNIVHDEMQIKKARNQLIHSLLKLNMYGIIHFQE"
     gene            complement(2230..3384)
                     /locus_tag="PLX22_11440"
     CDS             complement(2230..3384)
                     /locus_tag="PLX22_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT20550.1"
                     /translation="MSLLSQHNTTVPKNILVIDDSKLNRAIVKNTLLNYNIHVFEAAD
                     GLEGLTVLQSRHFDLILLDIVMPNMDGFGFLEQCKQYIGDDFIPIILMTGLDDLNSKI
                     KGLSIGADDFLLKPLNQKELIARVQSLLRLKQAHDELYEKNQQIQKELEYAKRLQQFI
                     IPKDFSFISYPSISGRYLPIADIGGDLFDCYKISDSKTGVLIADVTGHGIPAALVMSM
                     AKMTFNIHSYEIFPTQQLMSKINEEMRGLLLDMQYITAFYMLIDNEHKNIAFTNAGHT
                     RPLYYRKSTDKIIALDTNGLFLGIQDESLYEQKSIHYESGDRCILYTDGITELKDDHN
                     EEFGEVRLAKFLKQHNNASGSFCDNLLQTLEHFAPLNERSDDIAFLFIEF"
     gene            complement(3399..>3620)
                     /locus_tag="PLX22_11445"
     CDS             complement(3399..>3620)
                     /locus_tag="PLX22_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20551.1"
                     /translation="SDHIIRNIHYFKDRIKIINGLRLFCDQINVPIYAPNILKEGEYK
                     IIKDITVAYMSGPYIEQQFTLFSLNKKVV"
BASE COUNT         1219 a          523 c          599 g         1279 t
ORIGIN      
        1 cgtttagcaa catcgtcaac tactttgatt acaagaacat cttcattgaa tgctaccggt
       61 tcagtatttt taaaccacat attaaatgat tgcttattaa tataagcttc taccgaacct
      121 aatactttat tccagattgt tgttgaattt gtcataatac aaacctgttt agttattaac
      181 aattgttaat aactttgttg atatcataat atacgtttag catgcggaaa aaaggttaac
      241 tgagtgcttc agcataataa ctatagtacc tatgatactg tattgtttct acttcagcat
      301 aaaggtttta ttttgcaacc aattaataaa taaaattgat ttaacgaatg aattttttta
      361 ttattatgga tatagtgaga ataagtcctg atattgtgtg tataaaatgt taataaatca
      421 atatttatag ttgaaaaata ttaaatcatg tgtattaatt gttaataatt tttacattat
      481 aaaaaggaag taactctatg agcgttatca taaaggatat acagagaaat aacactgaga
      541 ttatacgagt ggaaatatca gaatttaaag gcaaagaact tattaatctt agaatctggt
      601 atcaatcaat tgagaatggc gcggttgttt ataaacccac acaaaaagga gtagcacttt
      661 cagttgacaa atttcatgag ctacaggaag cagtttcaaa aattgctgaa tatcttcatg
      721 ataaaaaatt tggtactact cctgaaaatg tataatgcca tacatgttta gtttcagcag
      781 ggagtgtatt agttgattcc ttgctttttt tatctgcatt tcgtcatgta caatgttagg
      841 atttaaaaca ccgttataga catcaataat acagtactgt gcaccctcag gaaaatgttt
      901 tatgcattct aaacataatt ttttagaaga ttgttttagt gcagttgcta taacaaacca
      961 tcctatttca aataatacat aatacatgtt ttcaggtgtc atatatttct ttaatagtat
     1021 attgaatggc tgagagtaca gtttattatg agagcttatt gtattcaaaa agagttcaat
     1081 cattctgtgc tgtgatgaga taatcagatt agtatcacat tccatttcaa aaagggatgc
     1141 tataccgttg atcctttttg atgaaatata gttatccaga agtgagatca aatgtctgtt
     1201 attgtaaaaa gctgctgcaa tggttatatc atcacatttt gcaaggactg catataaaag
     1261 attggatttt ataaattcat acacccattc atcaaaagac ttttgttgaa atagtgttac
     1321 cattcgcata tggtgtaaaa tgctatgaac ctgttcaatg aacgttccat aatggaaata
     1381 tggtttgcta cgtaatttca aaaataaaag ttcttcaaat gcatatattg cttcgagaac
     1441 atcccggtat gttattttat ggattgattt acccacattg atgatatctg aaatagtatg
     1501 cctggatata ttatgtttta tcttcagtga atattgaggg tatgctctgg aaataagaaa
     1561 aagatggtaa ggagttagta gcttttcatt taataaataa taaataagct tttggaattc
     1621 atcatcgctc atcacctcaa ggataaaggg caatgaagga aatagtgtat agggatcttt
     1681 gaaaaaatgt aaaaattgtg attcaagttc ataggtattg acaggtcgat gagaggtatc
     1741 catgtttatt ttaaaaagtt taaagaaatc attctcacaa taaattgaaa aggtgttatt
     1801 gctgtttcga tcatctgtat cccggataga tgctttaatc tcaatgtttg gcacttcatt
     1861 ttctcctttc gacaagaaat gattgcttat tagtgtaatg tttaatttat gtttcactat
     1921 caaactcaaa cgcagggcaa tagtgttgct attatcgtta agatactgca gtatgcaatg
     1981 gtgtactgat ggtgtgctgg tggatattat aatccggaaa ttgttttctg atgctattga
     2041 tttgtgaaaa gatactacaa tatattgatt tgttgtatcc aatttttcta tcatgaaagg
     2101 tgataatgct aaacctgaaa ggttaaaata actttttaga taagcatcga tcttatggaa
     2161 cagtatagaa aatataatat gagaagcggc attataatac atgtaaatta gtttaataat
     2221 tttattatat taaaattcaa taaataaaaa ggctatatca tcacttctct catttaaagg
     2281 tgcaaaatgt tctaatgtct gaagaagatt gtcacaaaaa gaacctgatg cgttattatg
     2341 ttgttttaaa aattttgcca gtctgacttc accaaattct tcattatggt catcttttag
     2401 ttcagtaatc ccatcagtgt ataaaataca tctatcacct gattcataat gaattgattt
     2461 ttgttcataa agtgattcat cctgaatgcc taaaaaaagt ccattggtat ccaatgcaat
     2521 aattttatca gtacttttgc gatagtaaag agggcgagta tggcctgcat tggtgaaagc
     2581 tatatttttg tgttcattat ctatgagcat ataaaatgct gtgatatact gcatatctaa
     2641 caaaaggcca cgcatttctt catttatctt tgacatcagt tgttgtgtag ggaatatttc
     2701 atatgaatga atattgaatg tcatctttgc catgctcatc accagtgcag cagggatccc
     2761 atgccctgtc acatctgcaa ttaatactcc agtctttgaa tcagaaattt tatagcaatc
     2821 aaagagatcg ccacctatgt cagcaattgg aagataccga ccactgattg acggataaga
     2881 aataaatgaa aaatctttag gtatgatgaa ttgctgcagc cgtttggcat attccaattc
     2941 tttttgtatc tgttgatttt tttcgtataa ttcatcatga gcttgtttca gcctcaataa
     3001 tgattgtaca cgggcaatga gctctttttg attcaatggc tttaaaagaa aatcatcagc
     3061 tcctattgat aagcctttga tctttgagtt taaatcatct aaacccgtca tcagaataat
     3121 aggaataaag tcatctccaa tgtattgttt gcattgttct aaaaaaccaa aaccgtccat
     3181 gttaggcata acaatatcaa gaagaattaa atcaaaatgc cgtgattgca aaacggtaag
     3241 tccttctaaa ccatctgctg cttcaaaaac atggatatta taatttaata gagtattttt
     3301 tactattgct ctgtttagtt tactgtcgtc gataaccaga atgtttttcg gtactgttgt
     3361 gttgtgttgt gatagtaaag acataatggc aacacctcct aaaccacctt tttatttaag
     3421 gaaaaaagag tgaactgttg ttcaatatat ggacctgaca tataggcaac ggtgatatct
     3481 ttgattattt tgtattcacc ttcttttaaa atattgggtg catatatggg cacatttatc
     3541 tggtcacaga ataaacgtaa tccattgata attttaattc tatctttaaa ataatgtata
     3601 ttacggataa tatgatcaga
//
LOCUS       DAOSOX010000224         3604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_25893_length_3604_cov_7.468019, whole genome shotgun sequence.
ACCESSION   DAOSOX010000224 DAOSOX010000000
VERSION     DAOSOX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25893_length_3604_cov_7.468019"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..650
                     /locus_tag="PLX22_11450"
     CDS             201..650
                     /locus_tag="PLX22_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOT20552.1"
                     /translation="MPRKLNIDIDYLINVMQMHDRLNEYYIDLETGKIVCNEEFPDDY
                     NLSEDDLLSEDVSIDYESDRYLWIPVFESSKAYKFMEDFIYELENEEARKKLVYAIQG
                     KKPFRRFKDVLQAFPDIEKQWYGFEEKRMKEYIVEWLHEYDIAIESS"
     gene            759..1652
                     /locus_tag="PLX22_11455"
     CDS             759..1652
                     /locus_tag="PLX22_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20553.1"
                     /translation="MINNELKERMEALINSREDWTVDELNQELEKIVNEMNKKPLDNF
                     EGFSPADMHYILYTPFHEKCPIGINTDISKDFLLQHSPLLKLAVYIMEKISAEGPMPL
                     TSKGNLKVSFIKELYNLGIYYGYDRDSFEKRPYQGNWDFLKVVMLTCIIAGVLKKIKG
                     ILDITKKGNALLTDNGSSLFKVLFIANTIKLNWGYFDFFKNDDCGQLGFLWTLWILKK
                     YGFTPKPASFYAQKYLTAFPIFINQQNNMHFSDVCFTVRFMARFCSYFGFIASDNNDN
                     TAFFLKNPEIVSTPLLNAFIK"
     gene            2249..2779
                     /locus_tag="PLX22_11460"
     CDS             2249..2779
                     /locus_tag="PLX22_11460"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOT20554.1"
                     /translation="MELRTVIRHIHGYAVGFTIFLVCLPAVIYFIASYFHQLIPAGII
                     GNTIIRVLVSVVLFLIGLIFMIWSNVALLVIGKGGPVDAFNVSISPRTKHLVVKGLYR
                     YTRNPMVFGALCCYIAFALYLNSLVVVVFILAFVPVIIIYLKQTEEKRLEQDFGQEFR
                     EYSKKVSMLLPLPPHK"
     gene            complement(2782..>3604)
                     /locus_tag="PLX22_11465"
     CDS             complement(2782..>3604)
                     /locus_tag="PLX22_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOT20555.1"
                     /translation="RMQFDTRATWILLEALVEMGYLQKDNDIYSPTQYCIDHLINEES
                     DDYDGDFLKFLLYLMHPWQTLPYVLKTGTPDESSFKELSMQDFIKGMDSPWKKRITPE
                     VVSLCLAYCTASSCIDIGGAPGTIAKEFVKRGVKTIIFDLPQSLEITQDELKDVPDIE
                     IIAGDATVALPHGKVDIAFLGNLCHGQSPQDNQKIISMCYDIVNPDGIIAIFDNLRDE
                     SYLGATLALHMLTQSKHGNIYAREEYFAWLHNAGFKEPQVLQLSDKAWQMIIAKK"
BASE COUNT         1112 a          581 c          705 g         1206 t
ORIGIN      
        1 atgagtacgg tgtttatggt atactcatgg gagtcattag agaacagaat gaaaggtgga
       61 cttataaaag tcactgggaa aaaagttaat tggtggcata ctgctctgac agattacaca
      121 ctaaaaagga cgtgacacac tatcttaata ttttcatcct aattatcaca actgtgctat
      181 acattaggag gaactatcgc atgccacgta aacttaacat tgatattgat tatcttatca
      241 atgtaatgca gatgcatgat aggttgaatg aatactatat tgatcttgaa acaggtaaaa
      301 tagtttgtaa tgaagaattc cctgatgatt ataatcttag tgaagatgat ttgctgtctg
      361 aagatgtatc tattgattat gaatctgatc gttatttatg gattcccgtt tttgaatcaa
      421 gcaaagcgta taagtttatg gaagatttta tatatgaatt agagaatgaa gaggcgcgta
      481 aaaaattagt atatgcaata cagggtaaaa aaccctttag gagatttaaa gatgtgctac
      541 aggcgtttcc agatattgaa aaacagtggt atggttttga agaaaaaagg atgaaagagt
      601 atatcgtaga gtggttacat gaatatgata ttgcaattga aagctcataa ttattttaat
      661 tggttgtgta tatatgaaca tttttttgtc cattatactg taccatataa attgcgatat
      721 tttgtttaca gctacattaa tctatcaagg gtaaatatat gataaacaac gaacttaaag
      781 aaagaatgga agcactgata aacagcagag aagattggac tgttgatgaa cttaatcaag
      841 agctggaaaa aattgtcaat gaaatgaaca aaaaaccatt agataatttt gaaggatttt
      901 ctcctgcaga tatgcactat atactttata ctccgtttca tgaaaaatgc cctattggta
      961 taaatactga tatttccaaa gattttttac tacagcattc acctttattg aagttagcag
     1021 tatatattat ggaaaaaatt agcgcagaag gtcctatgcc attaacatct aaaggtaatc
     1081 ttaaggtaag ctttattaaa gaattgtata atcttgggat ttattatggt tatgataggg
     1141 acagttttga aaaacgtcct taccagggca actgggattt tcttaaggtg gttatgctta
     1201 cttgtattat cgccggtgtt ttgaaaaaaa taaaaggaat acttgatatt acaaaaaaag
     1261 gtaacgcatt attgacggac aatggttcgt cgctctttaa ggtattgttt atagctaata
     1321 caattaaatt aaactggggg tattttgatt tttttaagaa tgatgattgt ggccaacttg
     1381 gatttttgtg gacattatgg attcttaaaa aatatggttt tacgcctaaa ccagccagtt
     1441 tttatgcgca aaaatattta acagcgtttc caatatttat taaccagcag aataacatgc
     1501 atttttcgga tgtctgtttt acagttcgct ttatggctcg tttttgtagt tattttggtt
     1561 ttattgccag tgataataat gacaatacgg catttttttt aaaaaatcct gaaattgtta
     1621 gtacaccact tttgaatgca tttatcaaat agttttggtt gattctttta tatacatttt
     1681 cactgctgtc ccataaaata aagggagaaa aataaaaaat gctattaatc tcactgttga
     1741 aagatattgt tggattacta aagtcctcaa taccccagca gttacaggag attaacagca
     1801 tgttcaaaag taaaacggta ttgtcacaaa tgcaatcatt aatttcagga tatgatttta
     1861 aaaatgcagt ttctgagtat aacggagaca agggggcaag gtattttcaa caaagaacct
     1921 gcttagtgtg atgctgtatg tgcatatggc ggcaaaacaa agtctaaggg acgttataga
     1981 ttccctggaa agcaaacgta atctctggta tcacatggga ctacaatcat tgtcacggaa
     2041 caatttatca tacgcattac agaacaggcc gcatcaaata tttgcgaaaa tatttttata
     2101 cattactggg acgttttcat aatgaaagag gtcgggtaca tgacaaaagg ttcaagttta
     2161 aaatgccgtt gcgaaccatt gattcaacaa ccgtaagact gtacaatact tcatcagtac
     2221 aataaaaaaa ttttagaagg tgaatagcat ggagcttaga acagttatcc gtcatattca
     2281 tggttatgcg gttggattta ctattttttt ggtatgtttg cccgcagtga tctatttcat
     2341 tgccagctat tttcatcaac tgatacctgc aggcatcatt ggcaatacta ttatacgggt
     2401 gctggtgtcg gtggtgctgt ttcttattgg gcttatcttt atgatatggt caaatgttgc
     2461 tttgcttgta attggcaaag gcggtcctgt ggatgcgttt aatgtttcca ttagtccgcg
     2521 tacaaagcat ctggtggtaa agggcctcta tcgttatacg cgtaatccta tggtttttgg
     2581 tgcattatgt tgttatattg catttgcgtt gtatctcaat tcgttagttg ttgttgtttt
     2641 cattctggcg tttgttccag tcatcatcat atatttaaag caaactgaag aaaaacgttt
     2701 agaacaggat tttggccagg aatttcgcga atattctaag aaagtctcaa tgcttttgcc
     2761 gctgccacct cataaataat gttatttttt tgctataatc atctgccatg ctttgtcgga
     2821 tagctgtaat acctgtggtt ccttaaatcc tgcattatgt aaccatgcaa agtattcttc
     2881 gcgtgcatat atattgccat gtttgctctg ggtaagcata tggagtgcca gcgttgctcc
     2941 taaatagctt tcatccctga gattgtcaaa gatagctata attccgtcag gatttactat
     3001 atcatagcac atgctgataa tcttctggtt atcctgcggc gattgcccat ggcacagatt
     3061 gcctaaaaaa gctatatcaa ccttaccgtg aggcagagca acggtggcat caccggctat
     3121 tatttctata tctggtacat cctttaattc atcctgtgtt atctccagtg attgcggaag
     3181 atcaaatatg atggttttaa ctcctcgttt tacaaattct ttggcgatag ttcccggtgc
     3241 tcctcctatg tcaatgcatg atgaagcagt gcaataagca aggcacaggc tgactacctc
     3301 tggagttatt cttttcttcc atggcgaatc catccctttt ataaaatcct gcatggatag
     3361 ctccttgaag cttgattcat caggagtacc ggtcttcagt acgtagggaa gtgtctgcca
     3421 tggatgcatc aggtataaca aaaatttcag gaagtcgcca tcgtaatcgt cgctttcttc
     3481 gtttatcaga tgatcaatgc agtattgtgt ggggctatag atatcgttat ccttttgcaa
     3541 atagcccatc tcaaccagtg cctctaataa tatccatgtt gcccgtgtgt caaactgcat
     3601 tcgc
//
LOCUS       DAOSOX010000225         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_26015_length_3591_cov_5.859446, whole genome shotgun sequence.
ACCESSION   DAOSOX010000225 DAOSOX010000000
VERSION     DAOSOX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26015_length_3591_cov_5.859446"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLX22_11470"
     CDS             <1..761
                     /locus_tag="PLX22_11470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT20556.1"
                     /translation="YEHADNETKKQFMFHHISTDEVYGDLPHPDDCKGNLPLFTETTA
                     YNPSSPYSASKASSDHIVRAWNRTYKLPVVITNCSNNYGPYQFPEKLVPLIILNAIEG
                     KPLPVYGKGDQIRDWLYVEDHAKALVLVATGKKTGQTYNIGGHNERRNIDVVTTICSI
                     LDEQYPVNQNEKIQEKKLPITSYSDLIHYVADRPGHDRRYAIDAGKIQKELGWKPEET
                     FESGLTKTVEWYLNNQWWWEPLRARYNRERLGVV"
     gene            758..1642
                     /gene="rfbA"
                     /locus_tag="PLX22_11475"
     CDS             758..1642
                     /gene="rfbA"
                     /locus_tag="PLX22_11475"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOT20557.1"
                     /translation="MKGIILAGGSGTRLYPITYPVCKQLLPVYDKPLIYYPLSTLMLA
                     GIQEILIISTPEDTPKFEKIFGNGNQLGLKLSYTVQPQPKGLAQAFILAEEFLAGDNA
                     CLVLGDNIFYGQGFTNILKRSKQCVENERKSVVFGYYVNDPQRYGVVEFDNHGNVLSI
                     EEKPEQPKSNYAVVGLYFYTADVVEIAKQVKPSWRGELEITTVNQMYLEKGKLKVELL
                     GRGYAWLDTGTFESMLEAANFIETIEHRQGLKIACIEEIAYNNKWITTEQLEKLAEPL
                     IKSGYGQYLKKLLQNIKV"
     gene            1681..2226
                     /gene="rfbC"
                     /locus_tag="PLX22_11480"
     CDS             1681..2226
                     /gene="rfbC"
                     /locus_tag="PLX22_11480"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOT20558.1"
                     /translation="MEFIKTELTGVFIVIPEVFKDNRGYFCEIYRDDLFKKNIGTTTF
                     VQDNESQSSYGVLRGLHYQLHPYAQSKLVRAVYGSILDIAVDIRKDSSTYGKHFSIEL
                     SHSNKKALFIPQGFAHGFIALSEYAIVQYKVDNFYSRAHDRGIDAFDGELAIDWKLPL
                     DVIKRSDKDKNLPCLREAEVL"
     gene            2223..3059
                     /gene="rfbD"
                     /locus_tag="PLX22_11485"
     CDS             2223..3059
                     /gene="rfbD"
                     /locus_tag="PLX22_11485"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOT20559.1"
                     /translation="MNILVTGAHGQLGSELNTMIDGIFTDVDELDITNSEMLSGFVER
                     HTITHIINCAAYTQVDNAEREKEQAFFINRDAVKNLANVARMHNIRLVHISTDFVFNG
                     SKNTPYTEDDMPDPISAYGASKYEGERAIVTSDIDYAIVRTSWLYSTYGNNFVKTIAR
                     LAKEKKSLRVVYDQVGTPTYASDLAQVLSKIVGDSSLKGVYHYSNEGVASWYDFAKAI
                     VDIMGIACTIEPIVSSDYPALAQRPPYSVMDKSKIKKQLGISVPYWRDSLQQCLNRLH
                     NG"
BASE COUNT         1162 a          594 c          829 g         1006 t
ORIGIN      
        1 attatgagca tgcagacaat gaaacaaaaa aacaatttat gtttcatcat atctcaacag
       61 atgaggtata tggggatctg ccgcatcctg atgattgtaa aggcaatttg cctcttttta
      121 ctgagacaac ggcatataat ccaagctcac cgtattctgc atccaaggca agctctgatc
      181 acatagtacg ggcatggaat agaacctata agcttcccgt ggtgataaca aattgttcta
      241 acaactatgg accatatcag ttccctgaaa agcttgttcc cctgataatt ttaaatgcta
      301 ttgagggtaa gccattgccg gtgtatggta aaggagatca gatcagagat tggctgtatg
      361 ttgaggatca tgcaaaagcc ctggtactgg ttgctacagg taaaaaaaca gggcaaacct
      421 ataacatcgg tggacataac gaaagaagga atattgatgt ggttacaacg atatgttcaa
      481 tacttgatga acagtatccc gttaaccaaa atgaaaaaat acaggaaaaa aaattaccaa
      541 ttacttcata tagcgattta attcactatg tagccgacag gccagggcat gatcggaggt
      601 atgcaattga tgcgggtaaa atccagaaag agttgggctg gaagccagaa gaaacatttg
      661 agtcggggtt aacaaaaaca gtagaatggt atctcaataa ccaatggtgg tgggaaccat
      721 tacgagcccg ttacaataga gagcgtctgg gggtggtatg aaagggatta tacttgcagg
      781 gggaagtggt acacgtcttt accctataac ctatccggtt tgtaagcagt tgttgcctgt
      841 gtatgataaa ccattgatat actatccatt gtcaacgctg atgcttgcag gcatacagga
      901 aatactcata atatcaacac cggaggatac tccaaaattt gaaaaaattt ttggtaatgg
      961 taatcagttg gggttgaaac tatcttatac agtacagcca cagcccaaag ggcttgcaca
     1021 ggcatttata ttagctgaag aatttttggc aggtgacaat gcctgtttag tactgggcga
     1081 taatatattt tatgggcagg gatttacgaa tattttaaaa agatccaaac aatgtgttga
     1141 aaatgaaaga aaatctgttg tatttggcta ctatgtcaat gatccacagc gctatggtgt
     1201 ggtggagttt gataatcatg gtaatgtatt aagcattgag gaaaagccag agcagcctaa
     1261 atcaaactat gcggttgtag ggctttattt ttataccgct gatgttgtag aaattgcaaa
     1321 gcaggtaaaa ccttcatggc gaggtgagct tgaaataaca acggtaaacc agatgtacct
     1381 tgaaaagggc aagctcaaag tagagctttt agggcgcggg tatgcatggc ttgatacggg
     1441 cacatttgaa agcatgctgg aggctgccaa ttttattgaa acaatagagc acaggcaggg
     1501 tttaaagata gcatgtattg aagagattgc gtataataat aagtggataa caacggagca
     1561 gcttgaaaaa cttgccgagc cattaattaa aagtggctat ggccagtatt taaaaaaatt
     1621 attgcaaaat ataaaggttt aatttgacaa tattaatatt taagttatag gggaattact
     1681 atggagttta taaaaacaga attgacgggc gtttttatag ttattcctga agtatttaag
     1741 gataatcgag gctatttttg cgagatatac cgggatgatc tcttcaaaaa aaacattgga
     1801 actacaacat ttgtacagga taatgaatca caatcatcgt acggtgtttt gcgaggatta
     1861 cactatcaat tgcatcctta cgcacagagc aagctggtac gtgcagttta tggaagcatt
     1921 cttgacatcg ctgttgatat acggaaggat tcgtctacgt atggaaaaca cttttcaata
     1981 gaactatcgc acagcaataa aaaagctctt tttataccgc agggatttgc tcatggattt
     2041 attgcacttt cagaatatgc catagtacag tataaggtag ataactttta ttcacgagca
     2101 catgacaggg gcattgatgc ttttgatggg gagcttgcta ttgattggaa acttccgctt
     2161 gatgtaataa agcgttcgga taaagataaa aacctgccat gcttgcgcga ggcagaagtg
     2221 ttatgaacat cctggttaca ggtgcgcatg ggcagttagg gtcagagctt aacaccatga
     2281 ttgatgggat ttttaccgat gttgatgagc ttgatattac caatagtgaa atgctcagcg
     2341 gctttgtaga gcgccatact attacccata ttattaattg tgcagcatat acgcaggtag
     2401 acaatgctga aagggaaaag gaacaggcat ttttcattaa ccgtgatgct gtaaaaaatc
     2461 ttgcaaatgt tgcacgcatg cataatataa ggctggtgca catttcaacg gattttgttt
     2521 ttaacggcag taaaaacaca ccttatactg aggatgacat gcctgacccc atttcagcgt
     2581 atggtgcatc aaaatatgag ggcgagcgtg caattgtaac gagcgatatt gattatgcaa
     2641 tagtgcgcac ctcatggctt tattcaacct atggcaataa ttttgttaag actatagcac
     2701 ggcttgcaaa agaaaaaaaa tcattacggg tggtatatga ccaggtgggc actccaacct
     2761 atgctagcga tttggctcaa gtactttcaa aaattgtggg cgatagttcc ttgaaagggg
     2821 tgtatcatta tagcaatgag ggtgttgcaa gctggtacga ttttgcaaaa gcaatagtgg
     2881 acattatggg catagcatgt accatagagc ctattgtatc cagcgactat ccagcgctgg
     2941 cacagcgtcc accctatagc gtcatggata aaagcaagat aaaaaaacag cttggaattt
     3001 ccgtccctta ctggcgcgat tcgttgcaac aatgtttaaa caggttacat aatgggtgat
     3061 ggtttattgg ttgatagtgg atggtaattt agttggtggt atatggtgaa tagttgattg
     3121 tgttataaag gaaatgttag atgttagatg taaagtggtg ttggggaaat gtatttgtgg
     3181 caatagtcat tctaaatgaa ggcaatgtcg ttctgagcgt tagcgcctgt catcctgaac
     3241 ttgtttcagg atctgtcgtt agtagataag attccgaatc aagttccaaa tgacatttta
     3301 aaaaacactc ccccctcgcc ttaccaagga gaggggctgg gggtgagatt gatatgtcat
     3361 cctaatccaa aggcgaaaaa tctcgttgca ttaagaaaga atagattctt cggacactgc
     3421 gtgctctttt agaatgacga aatgaaccaa tccaacgtcg tatagggtaa tagtatatac
     3481 ataaaaaata ctttgacatg ctcatagaaa atagtatact atatataaaa caggaggaat
     3541 tgtacccatg ggatatgcac taaggatata cgaagcattt aaggacgatg a
//
LOCUS       DAOSOX010000226         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_26186_length_3575_cov_9.760511, whole genome shotgun sequence.
ACCESSION   DAOSOX010000226 DAOSOX010000000
VERSION     DAOSOX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26186_length_3575_cov_9.760511"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLX22_11490"
     CDS             complement(<1..191)
                     /locus_tag="PLX22_11490"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HOT20560.1"
                     /translation="MSKSIEGVIASPGIVIGKAYIYRGDNIIIPKYTIPAEQVEKEIK
                     RYDESVKKTKEEIKAIQSQ"
     gene            676..1281
                     /locus_tag="PLX22_11495"
     CDS             676..1281
                     /locus_tag="PLX22_11495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT20561.1"
                     /translation="MVKKQIEARGVTDQRVLQAMLKVERHKFVPELSLSSAYEDHPLP
                     IGYGQTISQPYIVALMTELCELDGTEKVLEIGTGSGYQAAILSLLAKEVYTIEIIEPL
                     GKQAALRLKSLGYNNVTVKIGDGYKGWPEHSPFDVIILTAAPPTIPDALLNQLSEDGG
                     ILVAPVGDYNQELIKMVRHGNTYSQKVITYVRFVPMVPGKE"
     gene            complement(1285..2331)
                     /gene="argC"
                     /locus_tag="PLX22_11500"
     CDS             complement(1285..2331)
                     /gene="argC"
                     /locus_tag="PLX22_11500"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT20562.1"
                     /translation="MKNALIVGATGFGGLGLIEILLRHNGFKIKQLIARKDTGKKISE
                     VFPHMEGFCDMYVQSTEEIDYNDIDIAFFSTPDKAGMQLIQSFYQRNIPVIDFSGDFR
                     FNTINDYNIYAKNKGMNTEHAAPDILQHAVYGLPEKYAEKIKKAKIVGNPGCFAIAMI
                     LGLLPAVEEGIIDSETIICDGKTGVSGAGKNPGEANFYPQRYEDVNTYREGYHQHIVE
                     VENIVNAHAKKKHTIFFVPQIVPLNRGILVTIYADAIVTMDNEKLYDVYARYYKGKPF
                     IHITQRSCHTTDVRGTNLCLIRPAIDRRTGKLFITSVIDNLVKGQSGNAVQNANIMMG
                     FDETEGLMVPAFYP"
     gene            2562..3575
                     /locus_tag="PLX22_11505"
     CDS             2562..3575
                     /locus_tag="PLX22_11505"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT20563.1"
                     /translation="MKKYNVVILGATGAVGIEFRKILLQRNFPINTIRFLGKTTVGKT
                     IDFDDKKVTVEAVTDDAFKGFDIALFSAGASISREVARKAADSGCIVIDNSSAWRMEK
                     DVPLVVPEVNPDDVDWHNGIIANPNCSTIQMVVALKPIHDVAKIKRIVVSTYQAVSGT
                     GLKAIEELEIQTKDIMNGIPISQRKVYPHQIAFNVLPHIDVFLDNGYTKEEMKMVNET
                     KKIMRDSSIQVTATTVRVPVFYGHSESVNIETQKKITPIEVRELLQATKGITVVDNPS
                     KNEYPLAIYAAGKDDVFVGRIREDESIPNGINMWVVSDNIRKGAALNAIQIAELLIAK
                     KIV"
BASE COUNT         1197 a          594 c          659 g         1125 t
ORIGIN      
        1 atttgtgatt ggattgcttt tatttcttct tttgtttttt taactgattc atcatatcgt
       61 ttaatttctt tttctacctg ttcggcaggt attgtatatt ttggaataat gatattatcc
      121 cctctatata tataggcttt acctataaca atacccggcg aggcaataac cccttcaatt
      181 gattttgaca taataactaa tacctgtaat tactataaaa agattaatgt aaaaaaacta
      241 tcgcccataa taattctcag caccattaaa acactaaata agggtatact gctatattct
      301 atattaatgt aaatcaaatt ttacactgtt tcagaaaaag aattaaataa gtaatgtaaa
      361 aaagttgtca agataattaa taaattttat atttattata acgtgatagt taaaattaaa
      421 attttttgta tcagtatgga aaatccaatg gttacaattg atattttaca gtttttttaa
      481 ttattattca aacggaaaag ctgttttcca tgaatataaa tgtatacatg ggttattatg
      541 agttataaaa aaatgaattt acaaatcata tccattgttt ttataatagc cacattagcc
      601 atagctttta tatcaatatt ttatgctatt tctactggtg aaaggggatt atcattgcag
      661 cagcttcggg aattaatggt taaaaaacag attgaggctc gtggagtcac tgatcaacga
      721 gtactacagg ctatgctaaa ggtagagcgc cataaatttg ttccggaact aagtctgtct
      781 tctgcttatg aagatcatcc attacctatt ggttatgggc agacaatatc ccagccatat
      841 attgttgccc ttatgactga attatgcgaa ctggatggaa cggagaaggt tctggaaatt
      901 ggcacaggtt cagggtatca ggcagcaata ttatcattgc ttgcaaaaga ggtatatact
      961 attgaaatta ttgaaccact gggtaaacag gctgcgttac gattaaagtc attgggttat
     1021 aacaatgtga cagtaaaaat tggtgatggc tataaaggct ggccagagca ttcaccattt
     1081 gatgtaataa tacttactgc agcaccacca acaatacctg atgcattgtt aaatcagctc
     1141 agcgaagatg gcggtatcct tgtagcgcca gtaggtgatt acaaccagga attaattaaa
     1201 atggtgcggc atggcaatac ctattcgcaa aaagtaatta cctatgtacg ttttgtacct
     1261 atggttccag gaaaagaata atatttaagg ataaaatgca ggaaccatta atccttctgt
     1321 ttcatcaaat cccatcatta tatttgcatt ttgtacggca ttgcccgatt gtccttttac
     1381 cagattatcg ataaccgatg ttatgaatag ttttcctgtc cttctatcga tagcaggtcg
     1441 tatgaggcac aggttggttc cgcgcacgtc ggtagtatgg caggaacgct gtgtgatatg
     1501 aataaatggt ttgcctttat aatagcgggc atatacatca tacagctttt cattatccat
     1561 ggtaactatc gcatcagcat aaatggtaac taaaataccc cggttcaagg gtacaatttg
     1621 tggaacaaaa aaaattgtat gtttcttttt agcatgagca ttcactatat tttcaacttc
     1681 tacaatatgt tgatgatacc cttcacgata ggtgttgaca tcctcatagc gttgaggata
     1741 aaaattagcc tcaccaggat ttttgccggc acctgaaaca cctgttttgc catcacaaat
     1801 gattgtttct gaatcaataa taccttcttc aactgcagga agcaaaccca gtatcattgc
     1861 aattgcaaag caacccggat tgccaacaat ttttgctttt ttaatttttt cggcgtattt
     1921 ttcgggcaat ccataaacag catgttgtaa aatatcaggt gcagcatgtt ctgtgttcat
     1981 acctttattt tttgcataga tgttgtaatc attaatagta ttaaacctga aatcaccgct
     2041 aaaatcaatg acgggaatat ttctctggta aaatgattga ataagttgca taccggcttt
     2101 atcaggagtt gaaaaaaagg ctatgtcaat atcattataa tcaatctctt cagtggattg
     2161 aacatacata tcacaaaagc cttccatgtg agggaaaacc tctgaaattt ttttacccgt
     2221 atccttgcga gcaataagtt gttttatttt gaaaccgtta tgacgtagta aaatttcaat
     2281 aagccctaat ccgccaaaac ctgtagcacc aacaattaaa gcatttttca tacacacctc
     2341 gttgctattg acgttataat gttttgctgt actaaactat catagtattg tatactgtaa
     2401 aacaaaaaat ttatgggcga tagttacttt tgacgacaat accatcaaaa aaataaatgt
     2461 caattgggat aaaattttat tttaaaaaaa gttattattg acaaaaaaaa gtgatatata
     2521 tgaacaatac aaaacaccgt taatttcgat ggggggtcac tatgaagaaa tacaatgttg
     2581 ttattctggg cgccacagga gccgtaggga ttgaatttag aaaaattttg ctgcaacgca
     2641 attttccaat caatactatt cgttttttag gtaaaacaac tgttggtaaa acaatagatt
     2701 ttgatgataa aaaagtaact gttgaagcag taacagatga tgcattcaag gggtttgata
     2761 tagcactttt ttcagcagga gcttccataa gccgtgaggt tgcacgtaag gctgctgact
     2821 caggatgtat tgttattgac aacagttcag catggcggat ggaaaaggat gtgccgctgg
     2881 ttgtaccgga ggttaatcct gatgatgttg actggcacaa cggaataata gcaaatccca
     2941 actgctcaac catacagatg gttgttgcgc taaaacccat acatgatgtt gcaaagatta
     3001 aacgcattgt tgtcagcact tatcaggcag tttcaggaac tgggttaaaa gcaattgaag
     3061 aattagagat tcaaacaaag gatatcatga atggcattcc catttcccag cgtaaagtat
     3121 accctcatca aatagcgttt aatgtactgc cgcatatcga tgtctttctg gataatggat
     3181 atacaaaaga agagatgaag atggtaaacg aaactaaaaa gattatgcgc gatagttcta
     3241 tacaggtaac agcaacgaca gtacgcgtgc cggtatttta tggacattca gaatcagtaa
     3301 atattgaaac ccagaaaaag ataacaccca tagaagtaag agaattatta caagcaacta
     3361 aagggattac tgttgttgat aatccatcaa agaatgaata tcctcttgcc atatatgcag
     3421 cagggaaaga tgatgttttt gtgggaagaa tacgcgaaga tgaatcaatc cccaatggca
     3481 ttaatatgtg ggtggtttca gataatatac gtaaaggtgc agcactcaat gctatacaaa
     3541 tagctgagtt gcttattgca aagaagatag tttaa
//
LOCUS       DAOSOX010000227         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_26482_length_3545_cov_6.232092, whole genome shotgun sequence.
ACCESSION   DAOSOX010000227 DAOSOX010000000
VERSION     DAOSOX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26482_length_3545_cov_6.232092"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /gene="ptsP"
                     /locus_tag="PLX22_11510"
     CDS             <1..>1574
                     /gene="ptsP"
                     /locus_tag="PLX22_11510"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT20564.1"
                     /translation="DESVKKTKEEIKAIQSQIATSLSSDMADIFQSHLMVLEDPYMDQ
                     KVRETISKEKRNAEWVLNDFSLQLIQNLKSIEDEYLRERIIDIADINKRLINNLQKTH
                     DESLADLKEEVIIIATDLTPSDTAMMNKKYVLGFVTDRGGRTSHTAIMARALEIPAIV
                     GAQVATSLINHGDMIILDAIHGKIIVNPSKDEISEYEKYRDDLKQLENELATIIELPS
                     HTLDNETVYIYGNIEIPEEMNIIRDHGAQGIGLFRSEFLFLDKSLPDEERQFEEYKRV
                     VEFFTPHPVTIRTLDIGGDKIYAFTKQYKERNPFLGCRAIRFSLENEFLFRPQLRAIL
                     RVSALGNVKLMFPMISSVEEFLKAKSILHEEMENLDKAGIPFDKNLPVGIMIEVPSAA
                     INIDIFCKYCDFFSVGTNDLVQYTLAVDRISEKIAYLYNPLNLAVLRLLKQIADVSNQ
                     NNIPLSICGEMAGEPKYTMLLIGLGYRQLSMSSAFMYQIKRIIRSVSVKECIEMTDYL
                     LQLHNTYDIENAIMEV"
     assembly_gap    1575..1584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1609..2361
                     /locus_tag="PLX22_11515"
     CDS             1609..2361
                     /locus_tag="PLX22_11515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT20565.1"
                     /translation="MKLYHDLAEYYFVIEKGNRNINDDVNLILELSNTKKNLLLLDLG
                     CGTGEHLNELSKKGFICTGLDYSKNMLTHAQLRFPNAANFLLCDITNFDFYEEFDVII
                     SLFGSFDYMIDNDDVDKALWNTWRAMKSGGFGLFEIWNAIPVRKIKEKPLTTVSKVKY
                     KNMTIERQRGFTLIPDDNKTITEVNYIYTISENSKREVIKDKHIMRAFYKEEIESFFA
                     NNGLSIINIYANSKKETFNSLSNKILIHFMKE"
     gene            complement(2372..>3545)
                     /locus_tag="PLX22_11520"
     CDS             complement(2372..>3545)
                     /locus_tag="PLX22_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOT20566.1"
                     /translation="DKMSMDFRGDPSSALLEVLDPEQNNTFVDHYLEVPYDLSDVLFI
                     TTANVQHKIPAPLQDRMEIIEINSYTEPEKLNIALQFLLPKQIKENGLNESHIQYGND
                     AMLYTIRHYTREAGVRNLERVIAKVCRKVAREVVKNGPLHIRKITVDILHEYLGPIKF
                     KYGEKEQKSEIGLANGLAWTEVGGDVLQVEASLIKGKGQLILTGQLGEVMQESAHAAF
                     TYVRANSQYFNIPDDVLRKHDIHLHVPEGAIPKDGPSAGITMGVVIASLLSQIAVRCD
                     VAMTGEITLRGKVLPIGGLKEKVLAAHRAQIKHLIIPMDNEKDLEKIPEYVRKQLIFH
                     PVTSMEEVLFLSLEEPDKFFKDRQKTEKLTEQWKDTVRESVISDVTNELLTKHNYT"
BASE COUNT         1191 a          606 c          597 g         1141 t
ORIGIN      
        1 atgatgaatc agttaaaaaa acaaaagaag aaataaaagc aatccaatca caaattgcaa
       61 caagcctttc cagtgatatg gctgatattt ttcaaagcca tcttatggtt cttgaagatc
      121 cctatatgga tcaaaaagtt cgtgaaacaa tcagcaaaga aaaacgaaat gccgaatggg
      181 ttttaaatga tttttcatta caattaattc aaaatttaaa atccatagaa gatgagtatc
      241 tacgtgaacg catcattgat attgctgata tcaataagcg cttgataaat aatttacaaa
      301 aaacacatga tgaatcatta gccgatttaa aagaggaggt aatcattata gcaactgatt
      361 taacaccgtc cgatactgca atgatgaata aaaagtatgt gttaggcttt gtaactgacc
      421 ggggaggtag aacatcccat actgcaatca tggcgcgtgc attggaaata cctgcaattg
      481 ttggtgcaca ggttgctact tcactcatta atcatggtga tatgataata ttggatgcaa
      541 tccatgggaa aataattgtt aatccatcaa aagatgaaat cagtgaatat gaaaaatacc
      601 gtgatgatct taaacaactg gaaaatgaat tagctacaat catagaactt ccttcacata
      661 ccttagataa tgagacagta tacatttatg gcaatattga aataccagaa gaaatgaaca
      721 taatccgcga ccatggcgca cagggtattg gtttattccg atcagaattt ttatttttag
      781 ataaatcgtt gcctgatgaa gaaaggcagt ttgaagaata caagcgagta gttgaatttt
      841 ttaccccaca tcctgttacc atacgtaccc ttgatattgg tggggataaa atatatgcat
      901 ttaccaaaca atataaagaa cggaatccct tcttaggttg ccgtgccatt cgttttagtc
      961 ttgaaaatga atttttgttt agaccgcaat tgcgtgccat actcagggtt tctgctctgg
     1021 ggaatgtcaa attgatgttc ccaatgattt catctgtgga agagttttta aaagcaaaat
     1081 caattttaca tgaagagatg gaaaatcttg ataaagccgg catcccattt gataaaaatt
     1141 tgccggtggg aataatgata gaggttccct cggcagctat taatatcgat atattttgta
     1201 aatattgtga tttcttctct gttggtacca atgatctggt tcagtataca ttggctgtag
     1261 accgaatcag cgaaaaaatt gcttatcttt acaatccttt aaatcttgct gttctccgtt
     1321 tgcttaagca gatagcagat gtttctaatc aaaataatat accgctatct atatgtggcg
     1381 aaatggcagg tgaaccaaaa tataccatgc tccttattgg attgggatat cgacagttgt
     1441 cgatgagttc tgcttttatg tatcaaataa aacgtatcat acgttcagta agcgttaagg
     1501 aatgcattga aatgactgat tatcttttgc agcttcacaa cacctacgac attgaaaatg
     1561 ctataatgga agttnnnnnn nnnntaatca aattacaatc ataaaactat gaaattatat
     1621 catgatttag ctgaatatta ttttgtaatt gaaaaaggga atagaaatat taacgatgat
     1681 gtaaatctta ttctggaact atcgaacaca aaaaaaaatc ttttactcct tgatcttggt
     1741 tgtggtacag gtgaacacct taatgaatta tctaaaaaag gttttatctg tacaggattg
     1801 gattatagta aaaatatgtt aacccacgca caattacgat ttcccaatgc tgcaaacttt
     1861 ttactatgcg atatcaccaa ctttgacttt tatgaggaat ttgatgtcat tatttctttg
     1921 tttggttctt ttgactatat gattgacaat gatgatgttg ataaagcatt atggaataca
     1981 tggcgtgcaa tgaaatcagg tggatttgga ctctttgaaa tatggaatgc aatacctgta
     2041 agaaagataa aagaaaaacc attgacaaca gtatcaaaag ttaaatataa aaacatgaca
     2101 attgaacggc aacgtggttt tacacttatc cctgatgaca ataaaacaat caccgaggtt
     2161 aattatatct acactatatc tgaaaattca aaaagagagg ttataaaaga taaacatatc
     2221 atgcgtgctt tctataagga agaaattgaa agttttttcg ctaataacgg attaagcata
     2281 atcaatatct atgcaaattc aaaaaaagaa acatttaatt cactatccaa taaaatattg
     2341 attcatttta tgaaagaata aaatgtataa gctatgtata attatgtttt gtcaataatt
     2401 cattagtaac atctgaaatt acactttccc ttacagtatc tttccattgt tcagtaagtt
     2461 tttctgtttt ctgtctatcc ttaaaaaatt tatcaggctc ttctaatgaa aggaagagta
     2521 cctcttccat actggtaaca ggatgaaaga taagctgctt gcgaacatat tctggaattt
     2581 tttcaagatc tttttcatta tccattggta taattaagtg ttttatctga gctctatgtg
     2641 ctgctaaaac tttttccttt aaaccaccta tgggcaatac ttttccacgc agtgtaatct
     2701 caccagtcat tgcaacatca catctcaccg caatctgact gagtaacgaa gcaataacaa
     2761 cacccattgt tatgcctgct gaaggtccat ctttggggat agcaccttca ggtacgtgca
     2821 gatgaatgtc atgttttcgt aatacatcgt caggtatatt aaaatactgt gagtttgctc
     2881 gtacataggt aaatgcagca tgcgctgatt cctgcataac ttcaccaagc tgaccggtta
     2941 agataagctg acctttgcct ttaattaaag atgcttcaac ctgtaaaaca tcacctccaa
     3001 cctctgtcca tgccaaccca ttggcaagtc ctatttcact cttttgttcc ttttctccat
     3061 atttaaactt aattgggcct aaatattcat gaagaatatc aacagtaatt ttcctgatat
     3121 gcaaaggacc gttttttact acttcacgag caacttttcg gcaaactttt gctataacac
     3181 gctcaagatt tcgtacaccc gcttcacgtg tataatgtcg tatggtatag agcattgcat
     3241 cattaccgta ttgtatatgt gattcattta atccattttc tttaatttgc tttggcaata
     3301 aaaattgtaa tgctatattt aacttttcag gctcagtata gctgtttatt tcaatgattt
     3361 ccatcctgtc ctgaagtgga gcaggtattt tatgctgtac attggctgta gtaataaaca
     3421 gaacatcaga taaatcgtac ggaacttcca gataatgatc aacaaacgta ttgttttgtt
     3481 cgggatctaa aacttccaat aacgctgatg aaggatcacc cctgaaatcc attgacatct
     3541 tatca
//
LOCUS       DAOSOX010000228         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_27275_length_3464_cov_8.287181, whole genome shotgun sequence.
ACCESSION   DAOSOX010000228 DAOSOX010000000
VERSION     DAOSOX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27275_length_3464_cov_8.287181"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLX22_11525"
     CDS             <1..356
                     /locus_tag="PLX22_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT20567.1"
                     /translation="IDDKEFHRKQIIQILESEGYEIIAAASNGAEALKLYESLAADLD
                     LITTDLDMPKLDGYALMYELSHKKPKAKIVFISEETTKGVLSDLLKMGAADFILKPIQ
                     RGTILERIKAVLQKK"
     gene            463..1539
                     /locus_tag="PLX22_11530"
     CDS             463..1539
                     /locus_tag="PLX22_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20568.1"
                     /translation="MEENDKAYMHLSEQTRKINIAMHYTHGDMERARQMVSGNYNDLY
                     AIKVRFASSTLFGAFLLFFNTIYNSISGLSLIVSPSYTLLNIDPALDWILFEKEIAKQ
                     MQKDEYDHVLAKTFSDKMIKNFSTILQNELSRNIEIKNDIAIDNLFRKLIQETFALQR
                     VDSLVVYQSTSSLTMELNSITIPKLESVKVEGEQTQESEVIKADTPSGEYTPLVGVDG
                     VKLIIKATLILSPIKGKHISQLSAGDRIMISIIDSKPVAVKVAKALQAYQDEQFKPIP
                     ARITHVQCIPQKGYKIYGLIAKGIVVDITEEEENIKVALDPTYAATVQAAEEEAKPNY
                     IIIAIVAVTFLIVIGILYLLLNAN"
     gene            complement(1532..2299)
                     /gene="hisF"
                     /locus_tag="PLX22_11535"
     CDS             complement(1532..2299)
                     /gene="hisF"
                     /locus_tag="PLX22_11535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT20569.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFVNLSDAGDPVNNGMFYDAEGA
                     DELVFLDITASSDRRAIILKMVKDVAETVTIPFTVGGGIRTIDDVRMILENGADKVSI
                     NTQAVLMPQLITQCAMRFGSQCIVVAIDAKQNSAGWNVYLHGGKTKTDIDALQWAKQA
                     EALGAGEILLTSMDRDGTKAGYDLKLTQSIADIVHIPVIASGGAGTLEHLYEGFVYGK
                     ADAVLAASIFHYREYSIKQAKDFLASHGIPIRPVDFS"
     gene            2413..>3464
                     /locus_tag="PLX22_11540"
     CDS             2413..>3464
                     /locus_tag="PLX22_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT20570.1"
                     /translation="MSKKIEKHNTFHDFSSRYVPLINQYIDSFYNNKELSAKNHELKI
                     AYRLLQEYCTREGKRIRPLLLLLAFAGYGGLVKKINAMIPVASSLECMHAFLLIQDDI
                     IDGAVVRRGKPAFHILCDSSYYQYNANIGTWVAIVLADVVMSNALEMIATLESSRIKY
                     FLNEFAVMYERTALGQLLDIIWSHPETISDDGIAEMIATMKTAHYTVSGPLKLGYILA
                     GGNDSNELQRLTEMGYSLGFAFQMRDDIIGVFGDEKDMGKSASSDIAEGKYTVLVQQA
                     WNKLNEKDRTHFLDLLRKDEKSSEEIDTIKHYITKSGAVTVLHAIIDEHLQKVHAILP
                     QLQCNDFMKQCMLQFI"
BASE COUNT         1153 a          605 c          676 g         1030 t
ORIGIN      
        1 taattgatga taaagagttc catcgcaagc agattattca aatactggaa tctgaaggat
       61 atgagataat tgctgctgct tccaatggag ctgaagcttt gaagctatat gaatcactgg
      121 ctgctgatct tgatcttatt acaactgatc tggatatgcc aaagttagat ggatatgcgc
      181 ttatgtatga attaagtcat aagaaaccaa aagctaaaat tgtgttcatc agtgaagaaa
      241 ccacaaaagg ggtgctgtct gacttattaa aaatgggtgc tgctgatttt attttaaaac
      301 ctatccagcg tggaacaata ttggagcgga tcaaagcagt attacaaaaa aaatgatagt
      361 atactatgaa taccatcaat tccattttgc tattacattt taactgataa agtctattgg
      421 tataaaaaat tgatataaag cacttttcac aggatagtat ccatggaaga aaatgataaa
      481 gcttacatgc atctgtctga acaaacaaga aagataaata ttgcaatgca ttatacccat
      541 ggtgatatgg aaagagcccg ccagatggtg tcagggaatt acaatgattt atatgctatc
      601 aaggttcgtt ttgcatcttc aacactgttt ggagctttct tgcttttttt taatactatt
      661 tataattcaa tttccggatt atcgctcatt gtatcacctt cctatacact gctgaatata
      721 gatccggcac tggactggat actatttgaa aaagaaatag caaaacagat gcaaaaagat
      781 gaatatgacc atgttcttgc taaaactttt agtgataaaa tgattaaaaa ttttagtact
      841 attttgcaaa atgaattatc ccgaaatatt gaaataaaaa atgatatagc aatagacaac
      901 ctattcagga agcttattca ggagacattc gcattgcaac gtgttgattc acttgtagtg
      961 tatcaatcaa catcatccct gacaatggaa ttgaattcaa ttactatccc aaagttggaa
     1021 tctgtaaagg tggaagggga gcaaacacag gaatcagagg taataaaagc agatactccg
     1081 tcaggcgaat atacaccatt ggtgggtgtt gatggtgtta agcttattat aaaagcaaca
     1141 ttaattttat cacccattaa agggaagcat atttcacaat tatcagccgg agacagaatc
     1201 atgatttcta ttatcgatag taaacctgtg gcggtaaagg ttgcaaaggc attgcaagcg
     1261 tatcaagatg aacaatttaa acccatccct gcgcgcatta cacatgtcca atgtattcca
     1321 caaaagggat ataaaatcta tgggcttatt gcaaaaggca ttgttgttga tataactgag
     1381 gaagaagaaa atataaaggt tgcattggat ccaacgtatg ctgctacagt gcaggcagca
     1441 gaggaagagg caaaaccaaa ttatattata atagcaattg ttgcagttac atttttaatt
     1501 gtaataggaa ttctgtattt attgttaaat gctaactaaa atctaccgga cgaataggga
     1561 tgccgtgtga agctaaaaaa tcctttgcct gcttgatgct gtattctcgg tagtgaaaaa
     1621 ttgaagctgc cagtactgca tctgctttac cataaacaaa tccttcgtat agatgctcta
     1681 aagttccagc acctcctgaa gcaataaccg gtatatgcac aatatctgca atgctttgcg
     1741 ttaactttaa atcataacct gctttagtgc cgtcccggtc catgctggtt aaaaggatct
     1801 cacctgctcc cagtgcttcg gcttgttttg cccattgcaa tgcatctata tcggtttttg
     1861 ttttaccgcc gtgcagataa acattccagc cggcactgtt ttgtttagca tcaatggcaa
     1921 caacaataca ttgagatcca aatcgcatgg cacattgggt aataagctgt ggcattaaca
     1981 ctgcctgagt attaattgat actttgtcag caccgttttc taaaatcatc ctgacatcat
     2041 caatagtacg tatgccacca ccaacggtaa aaggtatagt aaccgtttcg gcaacatctt
     2101 ttaccatttt tagtatgatt gcccgtcggt cgcttgatgc ggtaatatca agaaacacca
     2161 gctcatcggc accttcagca tcgtagaaca taccgttgtt aacaggatca cctgcatctg
     2221 aaaggtttac aaaattaaca cctttaacaa ccctgccatc tttaacatcc agacagggta
     2281 taatacgttt ggctaacatt aatagcaact atcctataaa attttgtatt gacataaggt
     2341 ttagatcaaa agggacaatt gtatcaaaac tcatttgaca ataccatagt caatctatca
     2401 aatggaattc atatgtcaaa aaaaattgaa aaacataata cttttcatga cttttccagc
     2461 agatatgttc cgctcatcaa tcagtatatt gacagttttt ataataataa agaactatct
     2521 gccaaaaatc atgagctaaa aattgcatat agactgttac aggaatattg tactcgcgaa
     2581 ggcaagcgta tccgtcctct gttgcttttg cttgcttttg caggatatgg gggcttggtt
     2641 aaaaaaatta atgcaatgat tcctgttgca tcgtcacttg agtgcatgca tgcatttttg
     2701 ttgattcagg atgacattat tgatggtgca gttgtaaggc gtgggaagcc ggcattccat
     2761 atactgtgtg acagctcata ttatcagtac aatgcaaata ttggtacatg ggtggcgata
     2821 gttcttgctg atgttgttat gagcaatgca cttgagatga tagcaacact ggagagcagt
     2881 cgtataaaat attttttgaa tgaatttgca gtaatgtatg aacgtaccgc gttaggtcaa
     2941 ctgcttgata ttatctggtc acatcctgaa acaatcagcg atgatggcat agcggagatg
     3001 attgctacaa tgaaaacggc tcactataca gtatctggcc ctttgaaact gggatatata
     3061 ctggctggtg gtaatgatag taatgagttg caacggctaa cagaaatggg ctattcattg
     3121 ggatttgctt tccagatgcg tgatgatatc ataggagttt ttggtgatga aaaggatatg
     3181 gggaaatcag caagttctga tatagccgaa ggaaaatata ctgtactggt acaacaggca
     3241 tggaacaaat taaatgaaaa agacagaacg catttccttg atttattaag gaaggatgaa
     3301 aaatcaagtg aagaaataga tacaataaaa cactatataa caaaaagtgg tgctgtcaca
     3361 gtactccatg ctatcattga tgaacatctg caaaaggtgc atgccatatt gcctcaactg
     3421 cagtgcaatg atttcatgaa acaatgcatg ctgcaattta taga
//
LOCUS       DAOSOX010000229         3455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_27379_length_3455_cov_11.648824, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000229 DAOSOX010000000
VERSION     DAOSOX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27379_length_3455_cov_11.648824"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1562
                     /locus_tag="PLX22_11545"
     CDS             267..1562
                     /locus_tag="PLX22_11545"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT20571.1"
                     /translation="MNIMQSAKAAKKASVYLAAVTPNSKNNALQLIARELHNNVALIK
                     QANETDVERSKKENLPAPLLKRLVFDDAKINEVIAGIESLMTLPDPVGKTLLATRLDD
                     ALTLYKVSCPIGVIGVIFESRPDALVQISTLCLKSGNAVLLKGGSEARETNKTLADII
                     YNAGCKAGLPQQWLTLLETREDVSAMLKMDDYIDLIIPRGSNAFVKYIMDNSRIPVLG
                     HAEGICHVYVDSDADMQMALKVVTDAKVQYVAVCNAAETLLVHNNIAKTFLPLLKQEL
                     DKYNVAIVGCEQTQAIINVNPATDEDWKTEYLDYKLSIKVVADVDEAINHINTYGSRH
                     TDSIVTANRATAKKFLDLVDSGNVFWNCSTRFSDGFRYGFGAEVGISTAKIHARGPVG
                     LDGLVIYKYKLIGNGDIVADFVTGNRTFKHKKIDEDCPV"
     gene            complement(1563..3107)
                     /locus_tag="PLX22_11550"
     CDS             complement(1563..3107)
                     /locus_tag="PLX22_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT20572.1"
                     /translation="MKFSGGLFEKYQDSDYLTKRKAFLIFIFAIAVIVLLNAGVILSF
                     IFISVERGLNFLQSAVGASIFSLITIYYLKKGKLQLASNIFVIFCSLVVLAGLFTKAA
                     HVGFVTMVYFMFATMLFAAAFSTKIIASIIYGSYVVALITFFYINKGSVEGKLAEALT
                     VGLVDSSAALTLCYIIVMMTITSFQSVIRSLDKEKNISVDQINVMKNLHSFIQDASDK
                     MIAISQLISDKLQDFLKNIQDQASAVEEITASTQEVSDGFGNVNNNVNNQYSALKKLF
                     DTINALAQEIDLLKNRSVEISKAFESILAITQRGENAIQLVNENAKTLLDSSGKLTGI
                     MNILEDIFDKIQLLALNASIEAARAGEAGRGFAVVADEVNKLSEQSVMSLKEINVNIQ
                     SNIQSVTTTNSGVATIIDLFKNIVETLNTVSTGMQDIFNHIDNQEKIKEAIQLQATGS
                     QSLSQKIAEDTNRHNDIIAEISNSIASINTLIQNNMSVTEDISDTSYELSNMGNDLLG
                     KIKAQV"
BASE COUNT         1079 a          678 c          651 g         1047 t
ORIGIN      
        1 atgtttacct tgctcatgga tacatattta aaaaaaacca tcactatgta gtggtgcaat
       61 ctaccatgca ttattttttt tgatgcaaac tactacggct tatttttttt ggcgatagtt
      121 actcagtatc agtacactgc ctgtgaatgt gccaccgctt taacaataaa tgaaaaccac
      181 tccttttttt accggcaaaa aaacaattga cagtaactat cggtactata cccattatac
      241 aaaattcagc ataacgaggc agtatcatga acataatgca atccgcaaaa gcagcaaaaa
      301 aagcttcggt ttatttagca gccgttacgc ccaatagtaa aaataacgca ttgcaattga
      361 ttgctcgtga attacacaat aatgtagcgc tcattaaaca agccaatgaa accgatgtgg
      421 aaagaagtaa aaaagaaaac ctgcctgcac cgctgttaaa acgccttgtt tttgacgatg
      481 caaaaataaa cgaggttatt gccggtattg aaagtttgat gacattgccc gaccccgttg
      541 gcaaaacact gttagctacc cggcttgatg atgctctgac cctctataaa gtaagctgcc
      601 ccataggtgt tattggcgtc atctttgaat ctcgccctga tgcgctggtg caaatttcaa
      661 cgttatgcct taaaagcggg aatgccgtac tgctgaaagg aggcagcgag gcgcgagaaa
      721 ccaacaaaac acttgccgac attatttaca atgctggttg taaagccggc ctgccacagc
      781 agtggcttac actgcttgaa acgcgcgaag atgtaagcgc catgctcaaa atggatgatt
      841 atattgacct catcatacct cgcggctcaa atgcatttgt taaatacatc atggacaatt
      901 cgcgcatacc ggtgcttggg catgctgaag gtatatgtca tgtatatgtt gacagcgatg
      961 ccgatatgca aatggcgctg aaggtggtaa ccgatgcaaa ggtgcagtat gtggcggtgt
     1021 gcaatgcagc cgaaacactg ctggtgcaca ataacattgc taaaactttt ttgcctttac
     1081 tgaagcagga gcttgacaaa tacaatgtgg cgatagttgg ctgtgaacaa acacaggcaa
     1141 ttattaatgt taatcccgca actgatgaag actggaaaac cgagtatctt gattacaagc
     1201 tttcaattaa agtagttgca gatgttgatg aagccattaa ccatataaac acctacggct
     1261 cgcggcatac cgacagcata gtaacagcaa acagagcaac agcaaaaaaa tttcttgatc
     1321 tggttgattc cggcaatgtt ttctggaact gttcaacgcg cttcagcgat ggctttagat
     1381 atggttttgg tgcagaggtt ggtataagca cagcaaaaat tcatgcacgc gggccagttg
     1441 gtctggatgg gcttgttatt tataagtata agctcattgg caatggggac atcgttgctg
     1501 attttgtcac aggcaatcga acatttaagc ataaaaaaat tgatgaagac tgccctgttt
     1561 aattatacct gtgcttttat cttgcccagc agatcattac ccatgtttga tagctcatat
     1621 gatgtgtcgc tgatatcctc ggtaaccgac atattattct ggatgagtgt gttgatgctt
     1681 gctatcgaat ttgaaatttc ggctatgata tcattatgac gattggtatc ttcggctatt
     1741 ttttgtgata aactctgtga acctgttgcc tgcaattgta ttgcttcctt gattttttcc
     1801 tgattatcaa tatggttaaa aatgtcctgc atgccggtgc taacagtatt cagcgtttcc
     1861 acaatatttt taaatagatc aataatggta gcaacaccag agttggttgt ggtaacactc
     1921 tgaatatttg actgtatatt gacattgatc tcttttaaac tcattaccga ctgctctgac
     1981 aatttgttta cctcatcagc aacaacggca aaaccccttc ccgcttcgcc cgcacgtgct
     2041 gcctcaatgg aagcgttaag agcaaggagc tgaatcttgt caaatatatc ttccagtata
     2101 ttcattatac cagttaattt accggagcta tctaacagag tttttgcatt ttcattaaca
     2161 agctgtattg cgttttcacc ccgctgcgtt atagcaagga ttgactcaaa tgcttttgaa
     2221 atttcaaccg aacgattttt aagcaaatct atttcctgtg ccaatgcatt gatggtatca
     2281 aaaagttttt ttaatgcact atactgattg tttacattgt tgttgacatt gccaaaaccg
     2341 tcggaaacct cctgcgttga tgcggttatc tcctcaactg cggatgcctg atcctgaata
     2401 ttttttaaaa agtcctgcaa tttatcagaa attaattgtg atatggcaat catcttatca
     2461 gaggcgtcct gtataaatga gtgcagattt ttcattacat taatctgatc cacactaata
     2521 tttttttctt tatccagtga acgtataaca ctttgaaatg atgtgatagt catcatcact
     2581 ataatgtagc acagtgttaa agcagcagaa ctatcaacta aaccaactgt taatgcttct
     2641 gcaagctttc cctcaacaga acctttgtta atataaaaaa atgtaattaa tgccacgaca
     2701 tatgaaccat agataattga cgctattatt tttgttgaaa acgccgcagc aaacagcatg
     2761 gtggcaaaca taaaatacac catggtaaca aacccaacat gagcagcctt ggtaaaaagc
     2821 ccggcaagaa caaccagtga acaaaagata acaaaaatat ttgaagctaa ttgcagcttt
     2881 ccttttttta gataatagat agttatcagg gaaaaaattg aagcaccaac agcagattgt
     2941 aaaaaattta aacctctttc aacgctgata aaaataaatg ataaaataac ccctgcattt
     3001 aataaaacaa tgaccgctat tgcaaagata aatatcagaa atgctttacg ctttgttaaa
     3061 taatcgctgt cttgatattt ttcaaataat cctccagaaa acttcataaa cgtacctctg
     3121 taatagtata ttactgtatt attactgttt ttataatttg tatatgcttt ttacgcttgt
     3181 ccactgcagc gaatttagat accattactg ttaattagtc aagaataatt tatcgttacc
     3241 aattaaaata gtttcggaaa taatcgcaaa gcaggtagaa ctgcctatgg ctggagtgta
     3301 gtaacccata atatcctgaa ctgatttcag gatctcattg tggcaataga ggttccggag
     3361 caggtccgga atgccagtga ggaataataa atcattaacc ttatagtagc ttttttaatg
     3421 tttttagcca ttatggcatg gtatccttta ctatt
//
LOCUS       DAOSOX010000230         3430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_27643_length_3430_cov_8.955852, whole genome shotgun sequence.
ACCESSION   DAOSOX010000230 DAOSOX010000000
VERSION     DAOSOX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27643_length_3430_cov_8.955852"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1753)
                     /locus_tag="PLX22_11555"
     CDS             complement(155..1753)
                     /locus_tag="PLX22_11555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT20573.1"
                     /translation="MQSTYILTHDVGTTGNKTCLYKVDDKIKLVNSYLVEYPLYMLPD
                     GGVEQKADDWWNAVCKATKWVMKQSSVAPAKIAAMAFCAQMQGSVFIDAGGRALRNPM
                     SYLDQRATKQIEKHLYKGLIKIDKWNALKTIQSLVITGGLAATPKDPLWKYHWVKDNE
                     PEIFEKAYKWLDVKDYLIFRCTGKLGMTVDSANVTFIYDTRKNRLGWHKGLCTMFEVD
                     MKHLPPVFQSTDVIGTITKKAASELGLTPGIPVFGGGGDVPLTLVGSGCTDLYDTNIY
                     VGTSGWVSSNVDKRMVDIGNFIASILGAIPHHYVYVAEQETAGACLKWVRDHLALDEI
                     GVYLKARHIADKSKEYESLYDFLDSVVLQTPPGAGNLIFTPWLHGNRSPREDSYSRGM
                     FFNLSLATGKRQMIRSVLEGVAFHIRWMLEAMEKKIPMQHSLRFAGGGAKSDVWCQII
                     ADVTGRTIEAIENEINAGTIGATIVCAVGLGIMDNFTEAKKLVGLRTSYKPRAQYHDM
                     YNRNFKVFTQLYEKNKELFKLLNY"
     gene            complement(2075..3202)
                     /locus_tag="PLX22_11560"
     CDS             complement(2075..3202)
                     /locus_tag="PLX22_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT20574.1"
                     /translation="MIPIIAIPPLLMAAIAMYVAVSYGIMYSRRRNEPENLWFAIMCG
                     TIALYDIASAMLYISPSPFQAVLYQRFQFALLACFIIAITWFIASLVHIRTNIVYSIT
                     AIMVLFIISGFLLDSSYTLNPYNPYIKKFVFLGATITYNEADPGILYIAQYIAMLVAG
                     TYLMYKLFIWCRYGEVHIKILFASLLVFLLASINDVCVGKGMYHFIYILEYAYFIVII
                     SMAYILQSRFLTLHKEIEQVTYLFNQKLLEQQKQQKIDDAFKQKCISSSNSDKIQQAV
                     EYIHDNFCFAISREGLAAMVNLHPDTFSRLFKLYTGKSLPDYINELRVNYAKVLLAKG
                     NESIVTIAFKTGFESLSTFNRAFHKLLNMTPTIYRKNNRKF"
BASE COUNT         1068 a          683 c          649 g         1030 t
ORIGIN      
        1 cttgtttcat ggaatgcctc ctttaaaatt aatttaattt taacctcaca ttatgcccct
       61 ctccttcgta aggcgagggg agagttcaac ctcacccccg gcccctctcc ttcgtaaggc
      121 gaggggggat gacattaaac atatacagaa tgacttaata attgagtaac ttaaaaagct
      181 ccttattctt ttcatacaat tgagtaaaga ccttgaaatt acgattatac atgtcatggt
      241 actgtgccct tggcttatac gatgtgcgta atcctacaag tttttttgct tcggtaaaat
      301 tgtccattat gccaagccca actgcacata caatcgtggc accaatagtg cctgcattga
      361 tctcgttttc tattgcttca attgttctgc cggttacatc agcaattatc tggcaccata
      421 cgtctgattt ggcaccaccg ccagcaaagc gcaaactgtg ctgcatggga atcttctttt
      481 ccatcgcttc aagcatccag cgtatatgaa atgcaacgcc ttccagcacg ctgcggatca
      541 tctgacgttt accggtagca aggctcaaat taaaaaacat cccgcgtgaa taggaatctt
      601 cacggggtga acggttgcca tggagccatg gagtaaagat tagattgcct gcacctggag
      661 gtgtttgcaa aaccactgaa tctaaaaaat cgtatagtga ttcatattct ttacttttgt
      721 cagcaatgtg acgtgccttt aaataaacac caatttcatc cagtgcaagg tgatcgcgta
      781 cccattttaa gcaggcaccg gctgtttcct gttcggcaac ataaacataa tggtgcggga
      841 tagctcctaa aattgatgca atgaagttgc caatatcaac catgcgtttg tcaacgttgg
      901 aagaaaccca gcccgaggtg ccaacataga tattggtatc atataagtca gtgcaccctg
      961 aacccaccag agttaaaggc acatctcctc caccgccaaa aacagggatg ccgggtgtta
     1021 atccaagttc gcttgctgct ttttttgtga ttgttccaat aacatcggtt gactgaaata
     1081 ccggcggcag atgtttcatg tcaacttcaa acatagtgca taatcccttg tgccagccca
     1141 atctattttt gcgggtatcg tagataaagg ttacatttgc cgaatcaacg gtcatgccta
     1201 attttcctgt acatctgaat atcaaataat ccttaacatc cagccactta tacgcctttt
     1261 caaagatttc aggttcatta tctttgaccc agtgatattt ccacagcggg tcttttgggg
     1321 ttgcggcaag cccgccggta attaccagtg attgaatggt tttcaatgcg ttccatttat
     1381 ctatctttat taagccttta tagaggtgtt tttctatttg ctttgttgca cgctggtcca
     1441 gatagctcat ggggttccgt aacgccctgc ccccggcatc aataaatacc gaaccctgca
     1501 tttgagcaca aaatgccatg gcagctattt tagctggtgc aacactactt tgtttcatca
     1561 cccattttgt agctttacaa acagcattcc accaatcatc agctttttgc tcaacaccac
     1621 catccggcag catatacagc ggatattcca caaggtatga gttaaccagc tttattttgt
     1681 catcaacctt atataaacat gttttgtttc cggttgtgcc aacatcgtga gtcaatatat
     1741 atgtcgattg catcgcaaac ccccagaaga ttatatactt gaataaacat aatgtcactc
     1801 tctatggaca caataggctt cctgtcttta tatatatgcg aaatgtgcaa gcgatagctc
     1861 ctgaaatcat aattattatt atgaaatctg aaaccactgt gaaagcacat ctaacctgtg
     1921 tacaacaccg ttgcataaaa tttgatacaa acaaaaaata catgaaaact gctcgtgaca
     1981 ttcattgaca ttagcacagg ggcactgatt ccataacaac tatcgaacac aatagcttta
     2041 tttttgtatc attgtctatg caatatctga ttaatcagaa tttgcgatta tttttgcgat
     2101 agatagttgg ggtcatattg agcagtttat ggaaagcacg gttgaatgtt gaaaggcttt
     2161 caaatccggt tttgaaggcg atagttacta ttgattcatt accttttgct aataaaacct
     2221 ttgcgtagtt aaccctgagt tcattgatgt agtccggaag cgattttccc gtatataatt
     2281 taaaaagcct gctgaatgta tccggatgaa gattaaccat ggcagccagc ccttcgcgtg
     2341 agatagcaaa acaaaagtta tcatgaatat actcaacagc ctgttgaatc ttatcagagt
     2401 tggacgaaga gatacatttt tgcttaaagg catcatcaat tttttgttgc ttctgttgtt
     2461 ctaaaagttt ttgattaaat aaataggtaa cctgttcaat ttctttatgt aatgttaaaa
     2521 atcgcgactg taatatatat gccatggata taataactat aaaatatgca tattcaagaa
     2581 tataaataaa atgatacatg cctttgccaa cacagacatc attgatactt gcaagcaaaa
     2641 aaaccagtaa gcttgcaaat aaaattttga tatgcacttc gccatacctg caccagataa
     2701 aaagtttata cattaaatat gttccagcaa caagcattgc aatatattgt gctatataaa
     2761 gaataccggg gtcagcctcg ttgtaggtga tggttgcacc taaaaagaca aattttttta
     2821 tgtaaggatt gtatggattg agagtatatg aactatccag caaaaaacct gatatgatga
     2881 acaataccat aatggcggtt atgctgtaaa caatgtttgt acgaatatgc accagtgaag
     2941 caatgaacca cgttattgct atgatgaaac aggcaagtag tgcaaactga aagcgctggt
     3001 acaatacagc ctggaaaggt gatggggata tatagagcat agcagatgca atgtcgtaaa
     3061 gagcaatagt gccgcacatt atggcaaacc acaggttttc gggttcattc cttctgcgtg
     3121 aatacataat accatacgag acggcaacat acatggcgat agcagccata agaagaggtg
     3181 gtatagctat tataggtatc atggtaaata ctcagcattc attcatcagg gtctcatcaa
     3241 taaatattaa aaagtgatta ttcttttttg caagaaaata aaaaaaggtg tatataaaaa
     3301 atcttgaatt atgaattgag aagcattgtg aaggtgaagt ggctgtgtag tgtttacaaa
     3361 atacggaaga ggataaaaat tagtatattt tttatataaa gaatacttat atatgaaagg
     3421 atatatgaaa
//
LOCUS       DAOSOX010000231         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_28088_length_3388_cov_7.285329, whole genome shotgun sequence.
ACCESSION   DAOSOX010000231 DAOSOX010000000
VERSION     DAOSOX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28088_length_3388_cov_7.285329"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..268
                     /locus_tag="PLX22_11565"
     CDS             128..268
                     /locus_tag="PLX22_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20575.1"
                     /translation="MKSNTYCIDNRCSYWRNTEHIYSAFVSKNEELGKHLFSFKNFIA
                     LE"
     gene            complement(390..866)
                     /locus_tag="PLX22_11570"
     CDS             complement(390..866)
                     /locus_tag="PLX22_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20576.1"
                     /translation="MAKKPLNFQQSIIDFMRSKSDRMERELRVPGDWYFNDGDEQEIK
                     SWADEEAEKVWEKIKHNIFKLGCSGLRYELCPFCHYYGYEHNGCYKALKNPICVKCNY
                     GKRHGICIGEEGQVSEYKKILQSFEDSRISMYKFFTNEYYTELIEKIEKNNAKAIA"
     gene            complement(1058..1504)
                     /locus_tag="PLX22_11575"
     CDS             complement(1058..1504)
                     /locus_tag="PLX22_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOT20577.1"
                     /translation="MQCDKLIVGIGNRLRSDDAAGSIIAARLAQAGLCSIDTAYMPEN
                     YIGTIRRLMPKTLVIVDACDMNLPAGEVRRIDLSAIYGRIITTHGLPVHFLLQKLQEV
                     TPHITFIGIQPETIAIGDKVSVQMHHGIDYCITMLVNGKEIDIPLL"
     gene            1641..2840
                     /locus_tag="PLX22_11580"
     CDS             1641..2840
                     /locus_tag="PLX22_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT20578.1"
                     /translation="MISKGLIERIFQAASIQRWNDHVRPMEFAEIDKQAHKMILAFTI
                     AKFQEEEKPGCVNWRLLIEGGIIELLHRVIVTDIKPPIFHKIMKEKGRELNEWVFEQL
                     QDDIGSVKGNFRETFQRYLFDDNFAVFEKKILHASHYLATNWEFQIIYHSNTFLYDIE
                     KTRNEIESQIEYHIDLTGVQRILLHSDIARFISLCGQLRFQQRWAQTHRIPKTSVLGH
                     MLIVAILSYFCSCELNTCQRRMYNNFFGSLFHDLPEVLTRDIVSPIKRSIKGLDQLIK
                     EYELSEVNERIFPLIPQNWHDEMKYFIFHEFENKVYIDNSITMGISFDAMNTQYNHDI
                     YNPMDGEILKACDDFAAFLEASFSINYGIKPEGLVSARRSIYQKYKAKHLVIGDVDFI
                     ALFDYFY"
     gene            2865..3260
                     /locus_tag="PLX22_11585"
     CDS             2865..3260
                     /locus_tag="PLX22_11585"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOT20579.1"
                     /translation="MPRTIKNLLLNYRDSIGALLVALKHPHVPMHSKMLIIIALAYAL
                     SPIDIIPDFIPILGYLDDAVIVPLLFYVAIHSIPQPVFYQCKQQAKNTFKKSIGCMVL
                     GLLLAALFWGFMALLLFILIKTILRIVAV"
BASE COUNT         1016 a          686 c          572 g         1114 t
ORIGIN      
        1 tggctttttt aagttatttt ttattaaatt ttttttacaa aggaaaaatt ttacttgatt
       61 atttagtatt ttagcatata tttaattaca tgattgtttt taattccttt ttaataggag
      121 gaaaattatg aaatcaaata cctactgcat agataacaga tgctcatact ggaggaatac
      181 tgaacatatt tactccgcat ttgttagcaa aaatgaagaa ttgggcaaac atctttttag
      241 ttttaaaaat tttattgccc ttgagtaata gtataaagcc atcaaccata ttaacctctg
      301 atacattata cgcaaaaaat aaagagacgg tacacttgac cgtctcttta ttttttataa
      361 agatgtcgta atatactcag tactataaat caggcaatag cttttgcgtt attcttctct
      421 atcttttcaa ttaattcagt ataatattca ttagtaaaaa atttatacat gctgattcga
      481 gaatcttcaa aggactgtaa gatcttctta tattcgctta cctgtccttc ctcgccaata
      541 catattccat ggcgcttgcc atagttgcat ttgacacata ttggattttt taatgcctta
      601 tagcaaccat tatgttcata accataataa tggcaaaaag gacaaagttc atagcgcaat
      661 cccgagcaac ctaatttgaa gatattatgt ttaatctttt cccatacttt ctccgcttct
      721 tcatcagccc agctttttat ttcttgttca tcgccatcat tgaaatacca gtcacccggt
      781 accctgagct cccgttccat cctatcagac ttggatcgca taaaatcgat aatagattgc
      841 tgaaaattta atggtttttt tgccataaca atatcccttt aattatattt taattattac
      901 atataccttt cacttcagac tatcgaccag atttatcatt ccaaacctca ttttaacata
      961 atttaaatta atatatatgg aatccacttc ctgtttaata aagtataccc attcggtatt
     1021 ttttcaagct ttttattacc attattacaa ttatttttca taataaaggg atatcaattt
     1081 ccttgccatt gacaagcatt gttatgcaat aatcaatgcc atggtgcatc tgtacactta
     1141 ctttatctcc tatggcaata gtttcaggct ggataccaat aaaagtaata tgtggtgtta
     1201 cttcctgtaa cttttgtagc aaaaaatgta ccggcaaccc atgggtggta ataattctac
     1261 cgtatattgc tgataaatca atccgccgca cctcgcctgc cggtaaattc atatcacagg
     1321 catcgacaat aaccaatgtt tttggcataa gccttcgtat ggtgccaata taattttccg
     1381 gcatatatgc tgtatcaata ctgcacaggc cagcctgtgc taatcttgct gcaatgatac
     1441 tccctgcagc gtcatcgctc cgcaaacgat tcccaatacc cactatgagc ttatcacact
     1501 gcataaccca ttaccggtaa ctatcgccaa aaatttttgc atttgtgcca ctttccacaa
     1561 tcacctttaa atattttact tgcatttacc agcaattcaa gttttatttt gctatgcgat
     1621 agttactgga gatgtaatct atgatttcaa aaggtttaat tgaaaggata tttcaagctg
     1681 ccagcatcca gcgatggaat gaccatgtgc gcccaatgga gtttgccgag attgacaaac
     1741 aggcacataa gatgatactt gcctttacca ttgcaaagtt tcaggaagaa gaaaagcctg
     1801 gctgtgttaa ctggcgattg ctcattgaag gtggcattat tgagctttta cacagggtga
     1861 tagttaccga catcaaacct cctatattcc acaaaataat gaaggaaaaa ggcagggagt
     1921 taaacgaatg ggtttttgaa cagcttcagg atgatattgg ttcggttaaa ggtaatttca
     1981 gggaaacatt ccaacgctac ctgtttgatg acaactttgc agtatttgaa aaaaagattt
     2041 tgcacgcttc ccattacctt gccactaact gggagtttca gattatctac cacagcaata
     2101 catttttgta tgacattgaa aaaacacgaa acgagataga aagtcagatt gagtaccaca
     2161 ttgacctcac aggggtccag aggattttgc tccacagcga tatcgcacga tttataagcc
     2221 tgtgcgggca actgcgtttt cagcagcgct gggcacagac tcaccgcatc cctaaaacat
     2281 cggtgctggg gcatatgctt attgttgcaa tactgtcgta cttttgctcg tgcgagctta
     2341 acacctgcca gcggcgtatg tataataact tttttggatc gctcttccac gatctgcccg
     2401 aggtgctcac gcgcgatatt gtttcaccca ttaagcgttc cataaagggg ctggatcaac
     2461 tgataaagga atatgaactt agcgaggtca atgaacgtat tttcccatta ataccgcaaa
     2521 actggcatga tgagatgaaa tactttatct ttcatgaatt tgaaaacaag gtgtacatcg
     2581 acaactccat aaccatgggg attagctttg atgcaatgaa cacacagtac aaccatgata
     2641 tatataatcc tatggatggt gaaatactca aagcctgtga tgactttgca gcatttttag
     2701 aggcttcatt ttccattaat tatggaatta aacccgaagg gcttgtatct gctcgacgca
     2761 gtatttacca aaaatataaa gcaaaacatc ttgttattgg cgatgttgat ttcatagcac
     2821 tgtttgatta tttttattag acaattactt tttttcgcaa atgtatgcca aggactatta
     2881 aaaacctgtt attaaattat cgcgattcaa tcggtgcatt gcttgttgct ctgaagcatc
     2941 cccatgttcc catgcattca aagatgctca taattattgc actggcatat gcattgtctc
     3001 caattgatat aatacccgat ttcattccca tacttggcta tttagacgat gctgtgattg
     3061 tgccactgct gttttatgta gccattcatt caatcccgca gcctgtgttt taccagtgta
     3121 aacaacaggc aaaaaacaca tttaaaaaaa gcattggatg tatggtattg ggcttattgc
     3181 ttgctgcatt gttctggggt ttcatggcac tgcttctctt tatacttata aaaacaatac
     3241 tacgcattgt tgcggtatga tagcaattat taatcctgat acgcccaaac caattgttga
     3301 agggcttatg agccatggct ttactccggt aaccattccg ctatgtacca cggtacagcg
     3361 tcctattgcc ggtcatcctg atattcaa
//
LOCUS       DAOSOX010000232         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_28346_length_3363_cov_8.902358, whole genome shotgun sequence.
ACCESSION   DAOSOX010000232 DAOSOX010000000
VERSION     DAOSOX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28346_length_3363_cov_8.902358"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1904)
                     /locus_tag="PLX22_11590"
     CDS             complement(<1..>1904)
                     /locus_tag="PLX22_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOT20580.1"
                     /translation="KPYIVKMAYEVDREKQWSKTTRALVLPARYHNPAEVAIPLTWTK
                     GKLTFDCASMGKGRLIVQLLSHKKKMTLGEYTLSPVPFTTMAIAIEKNYSDAVLILNF
                     QSDSEEYAYCFVANAMIKHTDNTTIPNVVFISVDALRADAVHCIVPKYAITPNMDALA
                     KDGAAFVNHCVVANWTRPSTIAMLSSVYGSTTGVNIYYFQVSNQEKKYFYTQSGVVPL
                     PVLFKHHGYITRSIGNNAFILDHSGIGVDVGFDDMSEYERQWEDTMDIASEVEQWLAD
                     NKDKPFFLFVNFNAPHNAYIPPAQYLEPLKKSIKGVHPWFRRYLGEVAYTDDYIGRIV
                     AVLKSLKLYDNTIIVVTTDHGEVFHEAHSHSPYTNKKSIFTHGQTLFDEEMHVPLIIK
                     LPEKKITGAISVPYQVRNIDIAPTVCELAGIPIPQSYQGISLLPYIECSEHGDRPVYC
                     EGRLMYGVRADGYKYVEKFYGFGVKPYHWGGQHVSEYHELYDLQKDTDELYNIAGIDD
                     EKVSAMKKKLYALRFKQPGNVLYAKKNVTGSIAVKEGFFYTVESEGVVQQVHRQSCRF
                     KLKKGQRLIFSTIPARTQYFITTDGVVVLLGYGGIALPKHGNEYIFDTTNPLLQQPIK
                     SDAIAAFPDA"
     assembly_gap    1905..2004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2005..2525
                     /locus_tag="PLX22_11595"
     CDS             <2005..2525
                     /locus_tag="PLX22_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20581.1"
                     /translation="TITVEPFTDDTWFRIAENSQFIDKANIVRLPKLFFFLILIENKT
                     DTPVMIKDIVLRYDNTTTAALTTEELKLSLSSPAYEWIKVDELTVPQRLLYFNGTIQN
                     IDFAHDAIPCTLPFVPAGDSHINIIAFTWIPVNVRKFTIVVTAITPDSKKIVECNFIR
                     NEYRTKGKHFLQ"
     gene            2556..2852
                     /gene="gatC"
                     /locus_tag="PLX22_11600"
     CDS             2556..2852
                     /gene="gatC"
                     /locus_tag="PLX22_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT20582.1"
                     /translation="MKFDENVINKVCELSRLELTPREKQEFTMQLEDIIRYVEKINEL
                     DTINVQPADHIVPLSNVFRDDRINESLPVQEVQKIAPQFEKNHFVVPRIIEEME"
     gene            2849..>3363
                     /gene="thpR"
                     /locus_tag="PLX22_11605"
     CDS             2849..>3363
                     /gene="thpR"
                     /locus_tag="PLX22_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT20583.1"
                     /translation="MNRLFIALPIDEAIIQNLLPVHDVLGNFADVVKVVEPSQYHLTL
                     KFIGECEGSIARAIEHEFTQLSLPITRFDYTVKDIGMFPNIKHPAVIWTGIHTDERAL
                     SQMVTSTQIFAKKFGIKEEVRSFVPHLTLARIRKGRKLTEKINDTLQPFTSMEFGSAT
                     FNRIVLFSSKLT"
BASE COUNT          997 a          706 c          619 g          941 t
ORIGIN      
        1 tgcatcaggg aatgcagcaa tagcatcact tttgataggt tgctgtaaca atgggttggt
       61 tgtatcaaag atatattcat tgccatgttt tggtaacgca atgccaccat atcccagaag
      121 aacaacaaca ccgtcagtgg taataaaata ttgtgtccgc gcaggaattg tagaaaaaat
      181 gagtctttga ccttttttaa gcttgaagcg acagctctga cgatgtacct gttgcaccac
      241 accttctgac tccaccgtat aaaaaaaacc ttcctttaca gcaatgctcc cggtaacatt
      301 ttttttagca tagagtacat tgcccggctg tttaaaacgc agtgcataca gcttcttttt
      361 catggctgaa accttttcat catcaatgcc tgctatgtta tagagctcat cagtatcttt
      421 ctgcaaatca tataattcat ggtattctga aacatgttgc ccaccccaat gatatggttt
      481 aaccccaaag ccataaaatt tttctacata cttataacca tctgctctga ccccatacat
      541 aagcctgccc tcacagtaaa ccggacggtc cccatgctct gaacactcaa tgtacggtag
      601 caagcttatg ccctgatagc tctgtggaat gggaatgcct gccagctcgc aaacagtagg
      661 agcaatatcg atatttcgca cctgataggg aacagatata gccccagtaa tttttttttc
      721 agggagtttg atgataagtg gcacatgcat ctcctcatca aacaatgttt gtccgtgggt
      781 aaatatagat tttttgttgg tatagggact atggctatgc gcctcatgaa aaacctcacc
      841 gtggtcagtt gttacaacta tgatggtgtt atcgtaaagc tttaagcttt ttaaaacagc
      901 aactatcctg ccaatataat catcggtata ggcaacctcg ccaagatagc gtctaaacca
      961 tggatgcacc cctttgatgc tttttttcaa tggttccaga tactgcgcag gagggatata
     1021 agcattatgc ggtgcattaa aatttacaaa aagaaaaaac ggtttatcct tattgtcagc
     1081 aagccactgc tcaacctcac tggcaatatc catcgtgtcc tcccactgtc gctcatattc
     1141 tgacatatca tcaaacccta catcgacacc aatgcccgaa tgatccagaa taaaagcatt
     1201 gttgccaata gaacgcgtga tatagccatg atgcttaaaa agtaccggga gcggcactac
     1261 accgctttgc gtataaaaat attttttttc ctgattggac acctgaaagt aatagatatt
     1321 caccccggta gttgatccat acaccgaaga gagcatcgca atcgttgaag gacgcgtcca
     1381 gttagctacc acacagtgat tgacaaatgc agcgccatcc tttgcaagcg catccatgtt
     1441 aggggtgata gcgtactttg gcacaataca gtgtacggca tctgctctta gtgcatcaac
     1501 cgagataaat acaacattgg ggattgttgt attatcagtg tgtttaatca ttgcattggc
     1561 aacaaagcag tacgcatatt cttcagaatc actctggaaa tttagtatca gtacagcatc
     1621 ggaataattt ttttctatag caattgccat tgttgtaaac ggcaccggtg acagcgtata
     1681 ttcgcctaac gtcatctttt ttttgtgcga tagtaactgt acaatcagcc ttcccttccc
     1741 cattgaggca cagtcaaaag ttaatttccc ttttgtccat gtaagaggaa tagcaacctc
     1801 ggcaggatta tggtaccgag ctggtaaaac aagtgcccgt gttgtctttg accattgttt
     1861 ttccctgtca acttcatacg ccatcttaac gatatatggt ttttnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnttacca ttaccgttga accttttact gacgatacat
     2041 ggtttagaat agctgaaaac agtcaattta ttgacaaagc aaatattgta cgccttccaa
     2101 aactattttt tttcctcata ttgattgaaa ataaaactga tacccctgtt atgataaagg
     2161 atatagtatt gaggtatgac aacacaacaa ctgctgcctt aaccactgaa gaattaaaac
     2221 tatctctttc ttcgcctgct tatgaatgga taaaggttga tgagcttacc gtaccccagc
     2281 gcttactgta cttcaacggc actatacaaa atattgattt tgcacacgat gctatcccct
     2341 gtacactgcc atttgtgccc gcaggcgata gccacatcaa catcattgca ttcacatgga
     2401 taccggttaa tgtacgcaaa tttaccattg tagtaacagc aataacacct gatagtaaaa
     2461 aaatagttga atgtaatttc atacgaaatg aatatagaac caaaggtaaa cattttttac
     2521 aatgaccata cttatgtact ataaggagat aaaccatgaa atttgacgaa aacgtcatca
     2581 acaaggtatg cgaactttcc cggttagaat tgacacctcg ggaaaaacag gaatttacca
     2641 tgcagttaga agatattata cgctatgttg aaaaaatcaa cgagcttgat accatcaatg
     2701 tgcagcctgc tgaccacatt gtaccattat ccaatgtatt cagggatgat aggattaatg
     2761 agtcgcttcc tgttcaggag gtgcaaaaaa ttgctccgca gtttgaaaaa aaccattttg
     2821 tggtaccacg cattattgag gagatggaat gaacaggctt tttattgcgc ttcctattga
     2881 tgaggcaatc atacaaaatc tgctgccggt acatgatgta cttggaaatt ttgccgatgt
     2941 agtaaaggtt gttgaaccgt cgcagtatca tttaacctta aaatttattg gcgaatgtga
     3001 aggcagtatt gcccgcgcaa tagaacatga attcacacag ctttcacttc ccataacccg
     3061 gtttgactat acggtaaagg acattgggat gtttcccaac atcaaacatc cggcagtgat
     3121 atggacaggt atacataccg atgaaagggc actatcacaa atggttacca gtacgcagat
     3181 atttgcaaaa aaatttggca ttaaggagga agtccgcagt tttgtgcctc atcttacatt
     3241 agcgcgtatc cggaaaggaa gaaagttaac cgaaaagatt aacgatacgc tacaaccatt
     3301 cacctccatg gagtttggca gcgcaacatt taaccgcatt gtactgtttt cctcaaagct
     3361 aac
//
LOCUS       DAOSOX010000233         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_28408_length_3358_cov_10.550409, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000233 DAOSOX010000000
VERSION     DAOSOX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28408_length_3358_cov_10.550409"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1003)
                     /locus_tag="PLX22_11610"
     CDS             complement(14..1003)
                     /locus_tag="PLX22_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOT20584.1"
                     /translation="MEFQKLRVAFVLLLCTIGFGTTGYYIFENMKPFDAFYMTMITIS
                     TVGFHEVKPLTNIGRIITITIILSGVSISAYSIGKLLRLLIEGELRKEFWRKKLERTI
                     QSLSNHFIICGFGRIGKLICKELFERRVPFVVIENNSVAIDELDKLGYLYIDMDATND
                     EALIKAGIMKSKGIVTSVMSDADNVYIVLTARGLRGDICILSRGSDEKSEQKLLRAGA
                     NRVVLPYQIGGKRMAQVLTRPTVVDFLDMAMMDSELGLAMEELKVGHNSSMVRKTLVE
                     SNLRRDYGVIIVAIKKINGQMIFNPQSSEILDAGDVIVALGKKYLMEQMSKVL"
     gene            1335..2306
                     /locus_tag="PLX22_11615"
     CDS             1335..2306
                     /locus_tag="PLX22_11615"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOT20585.1"
                     /translation="MATYQHSTGTFIGKGGIEIFFQQWEVANPKAVLIIIHGLGEHSG
                     RYGNLIDALQNKGIAIFGLDHRGFGRSGGKRGHTDSFMDYIYDMKIFVNMIKDKNPNK
                     PVIMLGHSMGGVLAIKYALTHAEDLDALILSSPGLVPTIKVPAWKEKLAIFLSKRIPS
                     FTMPSGLDASTISRDKEVVKKYMEDPLVHDKVTPRFYVEMMKTIEECINRCGEIKMPL
                     LLFQGTADALVKDEASKIVYQKASSRDKTLKLFEGLYHETMNELEADRKKVLKVVTDW
                     ILAHSKKTSGVKHAAAPKTKTKAAAKKPKAPQKKVSKSNDKSKSKKK"
     gene            complement(2426..3274)
                     /locus_tag="PLX22_11620"
     CDS             complement(2426..3274)
                     /locus_tag="PLX22_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT20586.1"
                     /translation="MIINHNISAIFAHRTLKFHDWAITKDMEKLSSGMRINKAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQEVHDILQRVRVLAVQSANGIY
                     TPEDRQQIQVEVSELVDEIDRIASQAEFNKMKLLTGAFARLHPSASMWFHMGPNMHQR
                     ERVFIETMTTAALGLRNAVTLTFISISTPGKANAVIGLADEALRQISKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDTDMAEQMAEFTKNQILTQAATAMLAQANTKSQT
                     VLQLLK"
BASE COUNT         1095 a          648 c          550 g         1065 t
ORIGIN      
        1 ttttttatag ctattataat actttactca tttgctccat caaatatttc ttacctaaag
       61 ctactataac atcacctgca tcaagaattt ctgacgattg cggattaaat atcatttgac
      121 catttatttt tttaattgcc acaataataa caccataatc tcgtcgcaaa ttactttcaa
      181 caagagtttt cctaaccatt gatgagttat gaccaacttt taactcttcc attgccaatc
      241 ctaattctga atccatcatt gccatatcaa gaaaatcaac aacagttgga cgtgttaaaa
      301 cctgggccat acgcttaccc cctatttgat aaggaagtac caccctgttt gctcctgcac
      361 gtaacagttt ttgttctgac ttttcatctg aacctcttga taaaatacaa atgtcaccac
      421 gtaaccctct ggctgttaat acaatataaa cattatctgc atcagacatc accgatgtga
      481 ctatcccttt tgatttcata ataccagctt tgatcaaagc ctcatcgttg gtagcgtcca
      541 tatcaatata caggtatcct aacttgtcta attcatctat agccacagaa ttgttttcaa
      601 tgactacaaa tggaactctt ctttcaaata attccttaca aattaatttc ccaattctgc
      661 caaaaccaca tattataaaa tggtttgata acgattgaat ggtacgttcc aattttttcc
      721 tccaaaattc tttgcgtaac tcaccttcaa ttaacaagcg taaaagtttt ccaattgagt
      781 atgcacttat tgatacacca cttaatatga ttgtaatagt aataattctc ccaatattag
      841 ttaacggttt gacttcgtgg aaacctacag tgctaatagt tatcattgtc atataaaaag
      901 catcaaatgg tttcatgttt tcaaaaatat aataaccagt tgttccaaaa ccaatagtac
      961 atagaaggag aacaaatgca acacgtaatt tctgaaactc cataataccc ttctatacaa
     1021 taaattcatt atcaatgcaa tcatatatca atatcgtatt atagaatgta aataattttt
     1081 aaaattaaaa attttatttg atttttttta tataaattta agataatgaa aaattattat
     1141 tcttctttaa tatttattac tatatacatt atataagggg tttgggagct atattttcat
     1201 tatctgtctt aacgcacact aaaggtaaaa gctgcttttt taaattattc ataagatgga
     1261 aagcagttct ttgtagtacc tatataataa tgtacatgta gtaattaaaa cttttaaaaa
     1321 agtgaggtga gattatggca acatatcaac atagtaccgg tacatttatt ggtaaaggag
     1381 gtattgaaat cttttttcag caatgggaag ttgctaaccc taaagcagtt ttaatcatta
     1441 ttcatgggct cggagagcat agcggccgat atggtaacct tattgatgcc cttcagaata
     1501 aagggattgc aatctttggt ttagaccaca gaggctttgg ccgcagtggg ggtaaacgag
     1561 gacatactga tagttttatg gattatatat acgatatgaa aatttttgta aatatgataa
     1621 aagacaaaaa tcctaacaaa cccgttatta tgttaggcca ttcaatgggt ggtgttcttg
     1681 ctattaaata cgcgctgaca catgctgagg atctggatgc actaatttta tcatcccctg
     1741 gattggttcc aacaattaaa gtaccggcat ggaaagaaaa actggcaata tttttgtcaa
     1801 aaaggattcc ctcatttacc atgccatccg ggttggacgc ttcaactatc tcccgtgaca
     1861 aggaggtggt taaaaaatat atggaggatc cgcttgttca tgataaagta actcctcgtt
     1921 tttatgttga gatgatgaaa accattgaag aatgtataaa tagatgcggc gaaataaaaa
     1981 tgccactttt actatttcaa ggaactgctg atgcgcttgt taaagatgag gcaagtaaaa
     2041 tagtttacca aaaagccagc tccagggata aaacattgaa gctttttgaa ggattatatc
     2101 atgaaactat gaatgaactg gaggcagacc gtaaaaaagt actaaaggtt gtaactgatt
     2161 ggattttagc gcactcaaaa aaaacctctg gagtaaagca tgcagcagcg ccaaaaacta
     2221 aaacaaaagc tgcagcaaag aaaccaaaag cacctcaaaa aaaagtatcc aaaagtaatg
     2281 acaaatcaaa atccaaaaag aaataaacat gctaaacaaa gttaaaagaa caatacttac
     2341 taatgttaac caataatacg aataaagccg ggtgggagtg cccggcttta tttatagtgg
     2401 tgttacgatg ctatctcaga tttatttatt ttaaaagctg taatactgtt tgagactttg
     2461 tatttgcctg cgccagcatt gcagtagctg cctgagtaag aatctggttc tttgtaaatt
     2521 ctgccatctg ctcggccata tcagtatctc gtatgcggct ttctgatgcc tggatattct
     2581 catatgcatt catcaagccc ttagcagcat gttccaaccg gttgtaataa gcaccaagat
     2641 cagctcgttg ctttgaaatc tgccgcagtg cctcatcggc taatccaata acagcattag
     2701 ctttaccagg tgttgaaatc gatataaatg ttagggtaac agcatttcgc aaacccaatg
     2761 cagcagttgt cattgtttca ataaataccc tttccctctg atgcatatta ggtcccatat
     2821 gaaaccacat ggatgctgaa ggatgaagcc gtgcaaatgc accagttaaa agtttcatct
     2881 tattaaattc agcttgagaa gcaatacgat cgatttcatc aaccaattct gaaacttcaa
     2941 cctgaatctg ctgtctgtct tccggtgtgt aaataccgtt tgctgactgt accgctaaca
     3001 ctcttacacg ctgtagtatg tcatgaactt cctgcaaata cccttctgct gtctgaatca
     3061 tcgacatacc atcttctgtg ttacgttcag cctgccgtaa accctgaatc tgtgaacgca
     3121 ttttttcgga cacagctaac cctgatgcat catcaccggc tttattgatg cgcatccctg
     3181 aagaaagctt ttccatgtcc ttggttattg cccaatcgtg gaatttcaac gttcggtggg
     3241 caaaaattgc gctgatgttg tggtttataa tcattgtatc ctccttgaac aatatactat
     3301 catccgtgat agcattaact atataaatag ttcattccaa gattcgtctt atttcaga
//
LOCUS       DAOSOX010000234         3331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_28702_length_3331_cov_5.884005, whole genome shotgun sequence.
ACCESSION   DAOSOX010000234 DAOSOX010000000
VERSION     DAOSOX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28702_length_3331_cov_5.884005"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLX22_11625"
     CDS             <1..656
                     /locus_tag="PLX22_11625"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOT20587.1"
                     /translation="IVLFQNGWGNAEKFISYFDKERIFNARVMTGFMKQELYHVIITV
                     HADAIRMGSLYHGYGKRLLSLAEALTTGGMAAEVVNDVVKYIWAKMLYNCALNPLGAL
                     FNVEYGDLGKSEYTKAIMDDIITEIFETMHRAGYTTHFSNAAEYINVFYEKLLPPTAK
                     HRSSMLQDIQAKRKTEIDALNGAVVALAQHYGYEAKVNGIITGLIKFIESTYTGATC"
     gene            complement(922..2163)
                     /locus_tag="PLX22_11630"
     CDS             complement(922..2163)
                     /locus_tag="PLX22_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT20588.1"
                     /translation="MKRLLWCLSTILTCLTIISFAIAQTDFQPTQGKITASTVNIRNI
                     PSAGGVNIGTLKRSDVVNVIGRSQNKSEIEGKEDYWYKIQFNDGKKTITGWVFGGFIS
                     FELNLESGLRWKSATPSPGASLTAISTDTNGNIYAGTSSGDLFISFDYGKSWRKVVPQ
                     ALGVSIGHIKKIVIDDNMILIASKGDKNGGIWKSTNNGASWSQFTTSQGLPSNEVVDI
                     AIAPNKYIYAITSDKICVSNDGCANWKILDDSGLKGKYNVIAIDRSGIVFVGTNKGAF
                     KMKKLIDVFGKEDTSWIAVGQKSPNMGEVYAIAINPHANEIYVGCNKGLNKSNLDNLD
                     SWQGIGGQSIINDIMIEPDGRTSVATEYGLNISIDNGASWVTYKSENGLSNNYVYRIA
                     LHSKNKSIWLITGKDGINYSE"
     gene            complement(2185..3141)
                     /locus_tag="PLX22_11635"
     CDS             complement(2185..3141)
                     /locus_tag="PLX22_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOT20589.1"
                     /translation="MKITRFFVSLLLIACLVPFVACKGKTKTILNEVYLIQNPEDKKI
                     AYVPNSKLKRGEFVKVIDEKVVNQKKYYYVQIEDVDVKGWIAENYVHDGKLESVTVLT
                     DTDLYMRPSIKSDKIGKVFAGQIAFKIDKKGDFVLIQYPGKEGYVLKDKVGTGEIIIK
                     KVTIPGIGSATVNASSQLVLGEGRELEFDPRNAFDGSLQTAWCEGKSGDTGVGEWIQI
                     SFDQCINLEEVSIVNGWSKSEDLYKTNTRVAQLKITSNKGGEAVVELSDGIFDFQKFD
                     ISLKGSSFKFIITKVYPGKDSDTCISEISLKSSSNQCEDYYD"
BASE COUNT         1004 a          651 c          512 g         1164 t
ORIGIN      
        1 ctattgtttt gtttcagaat ggatggggaa atgcagaaaa atttataagc tatttcgata
       61 aagagagaat ctttaatgca cgtgtcatga ccggatttat gaagcaggag ctatatcatg
      121 tgattataac ggttcatgct gatgccattc gtatgggaag cctctatcat ggatatggta
      181 agagattact atcgttagca gaggcgctca ctacaggtgg aatggcagca gaagttgtca
      241 atgatgtggt aaagtatata tgggctaaga tgctttacaa ttgtgcgctt aatcccctgg
      301 gtgcactctt caatgtagag tacggtgatc ttggtaaatc agaatacaca aaagcaataa
      361 tggatgacat cataaccgag atctttgaaa caatgcacag ggctggatac accacgcatt
      421 tcagcaatgc agcggaatat attaatgtgt tctatgaaaa gcttttgcca ccaacagcaa
      481 agcatcgttc atccatgctt caggatattc aggcaaaacg aaaaaccgag attgatgccc
      541 tcaacggcgc agttgttgca ttagcacaac actatggata tgaggctaaa gtaaatggaa
      601 ttatcacagg tctgataaaa tttattgaga gtacctatac cggcgctacc tgttagcgtt
      661 acgcttattg ctttatcgaa agtcctgcta atgcagtaac aacatttttt gctaacttct
      721 atgacaaaat cattttcaga atatagaatt gttcaccact accatcattc ctgatgatgg
      781 aatctttttt taggttgtca atgattatag tactgtcatc ctatgtaaaa atagtaaagc
      841 aaccatcacg gttgctttac tattatctgt tgtattaata tgtattaata acgcaaaata
      901 aattaaacaa gcagggaaat atcattctga atagtttatc ccatctttgc cggtaattag
      961 ccagatactt ttattcttgc tatggagagc tatcctgtaa acatagttat ttgacaatcc
     1021 attttcagat ttgtaggtaa cccacgatgc accattatct attgatatat ttaaaccata
     1081 ttcagtagca acacttgttc taccgtcggg ttcaatcata atatcattta taattgattg
     1141 tccacctatt ccctgccagc tatcaagatt atctaaattt gacttgttta aacctttatt
     1201 gcatccaaca taaatctcat tagcatgtgg gttaattgct atggcatata cttcgcccat
     1261 attaggtgat ttttgtccca ctgctatcca tgaagtatct tctttaccaa atacatcaat
     1321 taactttttc atcttgaaag cgcccttatt agttccaaca aatactatac ctgacctgtc
     1381 aattgcaata acattatatt tcccctttaa accgctgtcg tccagtattt tccaattagc
     1441 acagccatcg ttacttacac atattttatc gctggtaata gcataaatat atttattagg
     1501 agcaattgct atatcaacca cctcattaga aggtaaaccc tgtgatgtgg taaactgtga
     1561 ccacgatgca ccattgtttg ttgacttcca gatacctcca tttttatccc ctttagaagc
     1621 aattaaaatc atgttatcgt ctataactat ttttttgata tgccctatcg atacacccaa
     1681 tgcctgaggt actacttttc tccacgattt tccgtaatca aatgaaataa agaggtcccc
     1741 cgatgatgtt cctgcataaa tgttaccatt ggtatccgta gaaattgctg ttaaagaagc
     1801 tcctggagat ggagtagcgc ttttccatcg caaaccactt tcaagattta actcaaacga
     1861 tataaatcca ccaaataccc accctgtaat agttttttta ccatcattaa actgaatctt
     1921 gtaccaatag tcttctttac cttcaatttc tgatttattc tgtgaacgtc ctataacatt
     1981 aacaacatcg cttctcttta aagtgccaat atttacccct ccagcagatg gtatattacg
     2041 aatgttgact gtactggctg ttattttacc ctgggtaggt tgaaaatccg tctgggctat
     2101 agcaaatgat atgatcgtta aacatgttag tatcgttgac aagcaccata aaagtctttt
     2161 cattattccc ctccatttgt aatattaatc ataataatct tcacattgat ttgatgaact
     2221 tttgagtgat atttcactta tacatgtgtc agaatcttta cccggatata ctttagtaat
     2281 aatgaattta aatgatgatc cttttaagct aatatcaaat ttttgaaaat caaaaatccc
     2341 atcagataat tcaaccactg cttcgccacc tttattagat gtaatcttta actgtgctac
     2401 tcgtgtattg gttttatata agtcttcact ctttgaccaa ccgttaacta tgcttacctc
     2461 ttcaagattt atacattgat caaacgaaat ttgtatccat tcgcctaccc ccgtatctcc
     2521 ggatttccct tcacaccatg cggtctgcaa gctgccatca aaagcatttc ttggatcaaa
     2581 ttcaagctct cgaccttcac ctaaaactaa ttgtgatgat gcattgacgg ttgcacttcc
     2641 aattccaggg atagttactt tctttataat aatttctcca gttccaactt tatctttgag
     2701 cacatatcct tcttttccag gatattggat aaggacaaaa tcaccttttt tatctatttt
     2761 aaatgcaatc tgtccggcaa agactttgcc aattttatca cttttgatag acggcctcat
     2821 ataaaggtcg gtatcggtaa gtacagttac agattcaagt tttccgtcat gtacataatt
     2881 ctcagcaatc cagcctttga catcaacatc ttctatctgg acatagtaat actttttttg
     2941 gtttactact ttttcatcaa tgactttaac aaattcacct ctttttaatt tactgttggg
     3001 gacataagca atttttttgt cctcaggatt ctggattaaa taaacctcgt ttaagattgt
     3061 ttttgttttt cctttacatg ctacaaacgg taccaaacaa gctattaata gtaatgaaac
     3121 aaaaaatctg gtaatcttca tggacgtctc cttttgttaa ttttagtatt attgtttatc
     3181 aggattttat taacatttgt ttaatagtta caaattattt gtcaattaaa attttatatt
     3241 ttcctttttt tcgtaatctg tacataaact ttttttataa aaaagtgctg gattttagca
     3301 aagttgtatt agcatgtatg tcatttattt a
//
LOCUS       DAOSOX010000235         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29101_length_3296_cov_8.444924, whole genome shotgun sequence.
ACCESSION   DAOSOX010000235 DAOSOX010000000
VERSION     DAOSOX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29101_length_3296_cov_8.444924"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="amrS"
                     /locus_tag="PLX22_11640"
     CDS             complement(<1..781)
                     /gene="amrS"
                     /locus_tag="PLX22_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT20590.1"
                     /translation="MQCVLCPHNCIIKEGKTGICGVRQNKDGTLYSLIYEKPCAVHVD
                     PIEKKPLYHFMPGSKSYSLGTVGCNLKCMHCQNWELSTSLPGEVFCNTLTSDDCVKLA
                     LQYECASISYTYNEPGINFEYVLETAIKAHEKGIKNVMVTNGYINEEPLRELYQHIDA
                     ANVDLKAFDDDFYKTVCKARLQPVLKTLTILKQMNVWIEVTNLVIPTYNDDSKSIQML
                     VDWFVEHLGTQTPLHFTAFHPSHRMTNIPPTSLRILKNAYDI"
     gene            complement(845..>1357)
                     /locus_tag="PLX22_11645"
     CDS             complement(845..>1357)
                     /locus_tag="PLX22_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20591.1"
                     /translation="GQYDKALEMYKMAVSKYPNYILGLRNLGIGYRNLNRLDEALEAF
                     FKALKLKKDDAQTLSEIGYVYLLKAEYNKATDYFKQSLRYDPSYRYSLANMGWCLVQL
                     GKNNDAVPYLEKAHNDNSDYAWPAGYLGWLYENKFQDTNKALYWYGEALKRDPNNQDY
                     INAYNRLSGN"
     assembly_gap    1358..1457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1458..2368)
                     /locus_tag="PLX22_11650"
     CDS             complement(<1458..2368)
                     /locus_tag="PLX22_11650"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOT20592.1"
                     /translation="MKSFKKAVVVLIIGIACIANAKPGKKVIGVTAFTNLSKDTAYDW
                     LCIGIADTISYKLRNVQEYILVDRVNVDKIIGELQLSQSGLIDENTVEAGKALGASIL
                     VVGNFQKSGNQIRITAKIVDVQSHAVINQVQVTGTINDIFTLQDSIALGIIDKNDTAI
                     TTETKNKIVQNDTNNISAYEYFVRGQKYLLYQLNYSKAIEMYQKAISIDANYAVAYAG
                     LGKAYSLRSWELREYYNTTDPALIEKSFQSSKKALALNPNLDEAHLSLARYYQEVDEK
                     KIPNKWTLCEEETRKALEINENNGEAYL"
     gene            complement(2517..>3296)
                     /locus_tag="PLX22_11655"
     CDS             complement(2517..>3296)
                     /locus_tag="PLX22_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT20593.1"
                     /translation="LTPVIIEPFMVPKALKWTMLEMERRYYLLSEYNTRDIDRYNQMV
                     ERNKSGEYLPYIVIIMDELADLMMVAAKEIEGYITRIAQKARAVGIHLVLATQRPSVD
                     IITGIIKANFPARIAFQVAQKTDSRTIIDQNGAEKLLGKGDMLYQSPMSSFPIRIQGA
                     YISEDELSSVVLFLKKIANPHYVDIEQSLMEFDDDDEESEDDLFIEAVKIVETTKKAS
                     ASYLQRRLSIGYNRAARIIERMEELGYIGPQQGSKPREVYI"
BASE COUNT          896 a          635 c          597 g         1068 t
ORIGIN      
        1 ctatatcgta agcatttttt aaaattcgca gagaagttgg tggtatgttt gtcatcctgt
       61 gggatgggtg aaaggcggta aaatggagag gcgtttgtgt acccaggtgc tccacaaacc
      121 agtcaacaag catttgaatt gacttgctgt cgtcattata ggttggtata acaagattgg
      181 taacctcaat ccatacattc atctgtttaa ggatggttaa tgtttttaaa acaggttgca
      241 gccttgcttt gcatactgtt ttataaaagt catcatcaaa tgccttgaga tcaacatttg
      301 ccgcgtctat atgctggtac agttcacgaa gaggctcttc attgatatag ccattggtaa
      361 ccatcacatt ttttataccc ttttcatggg cttttatagc agtttcaaga acatactcaa
      421 agtttattcc tggttcatta taggtatacg atatagaagc acattcatat tgcaacgcta
      481 actttacgca atcatcagag gtaagcgtat tgcaaaaaac ttctccgggc aggctggtag
      541 ataattccca gttttggcaa tgcatacatt tgagattgca tcctacggtg cccagtgaat
      601 acgatttgct ccctggcata aaatggtata gcggtttttt ttctatgggg tcaacatgga
      661 ctgcgcaagg tttttcatag atgagagagt atagtgtgcc atctttattt tggcgtacac
      721 cgcatatacc tgttttccct tccttgatga tacaattgtg aggacataat acacattgca
      781 cactgtcccc atcatgtaag tgataatact gagcctcttt catgaagcgt ttaaaccttt
      841 agcgttagtt acctgataaa cggttatagg cattaatgta atcctgatta ttgggatcac
      901 gctttaaagc ttcaccgtac cagtagagag ctttgtttgt atcctgaaat ttattttcat
      961 aaagccagcc taaatatcct gctggccatg catagtcgct attatcatta tgagcttttt
     1021 ctaaataggg tactgcgtca ttatttttgc ccagttgaac cagacaccat cccatgttag
     1081 ctaaactata ccggtatgaa ggatcatacc gtaacgattg tttaaagtaa tctgttgctt
     1141 tgttatattc agcttttaat agatatacgt atccaatctc gcttaatgtt tgtgcgtcat
     1201 ccttctttaa ctttaaagct ttgaagaagg cttcgagagc ttcatcaagc ctgtttagat
     1261 tacggtatcc tattcccaaa tttctcagcc ccagtatgta gttagggtat tttgatacag
     1321 ccattttgta catttccaat gccttatcat actgtccnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnaga tatgcttcac cattattttc attaatttct aaagcctttc
     1501 gggtttcttc ttcacataat gtccatttgt tgggtatctt tttttcatca acttcctgat
     1561 aataacgggc taaagaaagg tgagcttcgt ctaaatttgg attaagggct aacgcttttt
     1621 tgctgctctg aaatgatttt tcaatgagtg caggatcagt ggtgttgtaa tattctctca
     1681 gttcccatga acgcagcgag tacgctttgc caagcccggc atacgcaaca gcataattgg
     1741 catcaataga gatggctttc tgatacatct cgatggcttt tgaataattt agctgataca
     1801 ataaatattt ttgtcctcgt acaaaatatt catacgcact aatattattg gtatcatttt
     1861 gaacaatttt attttttgtt tcagtggtaa tggctgtatc atttttatca atgattccca
     1921 gtgcaatgga atcctgtaag gtaaaaatat cgttgatagt tcctgttacc tgtacctggt
     1981 taataacagc atgtgactgc acatctacaa tttttgctgt tatgcgaatt tgattgccag
     2041 atttctggaa attaccaacc accaggatgc ttgcacctaa tgctttgcct gcttctacag
     2101 tgttttcatc tataagtccc gattgcgaaa gctgtaactc gccaattatc ttatcaacat
     2161 taacgcggtc cacaagaata tattcctgaa catttctgag tttatacgaa atagtatcag
     2221 caatacctat acatagccag tcatacgctg tatcttttga aagattggta aatgcagtga
     2281 cacctattac ttttttgcct ggtttagcgt tggctataca tgcaatgcca attatcaaaa
     2341 caactaccgc ttttttaaag gatttcatag accctctcat actgataaat gtgggatgat
     2401 agtaacatta aaatgaagcg tattgtatca ttacattttt ctaaaataac aataaagtaa
     2461 ctttatttat agataatata ctaaaggtgg ttgaaaaagt catgcaaaaa attaatttat
     2521 atatatactt ctcgtggttt acttccctgc tggggaccaa tatagcctaa ttcctccatc
     2581 cgctcaatga tgcgggctgc tctgttgtaa ccaatagata aacgtcgctg aagatatgaa
     2641 gctgatgcct ttttagtggt ttcaacaatc ttcacagcct caatgaaaag gtcatcttca
     2701 ctttcctcat catcgtcgtc aaattccata aggctctgtt caatgtcaac ataatgggga
     2761 ttggctattt tctttaagaa taacactact gatgacagct catcttcaga gatataggca
     2821 ccctgtatac gtatgggaaa agagctcatc ggcgactggt agagcatgtc gcccttcccc
     2881 agtagctttt cagcaccatt ttgatcaatg atggttcggg aatcagtctt ctgtgcaacc
     2941 tgaaatgcta tccgagcggg gaagtttgct tttatgattc cggtaatgat atcaacggac
     3001 ggccgctggg ttgcaagtac cagatgaatg cccacagcac gtgctttctg agcaatgcgt
     3061 gtaatatatc cttctatctc tttagcagca accatcatca agtcggcaag ttcatccata
     3121 ataataacga tgtacggtag atattcaccg cttttatttc gctctaccat ctgattgtag
     3181 cggtcaatat cacgggtatt gtattcagaa agcagatagt agcgacgctc catctctaac
     3241 atggtccact ttaatgcttt aggtaccata aaaggctcaa taatgacagg tgttaa
//
LOCUS       DAOSOX010000236         3294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29131_length_3294_cov_8.241741, whole genome shotgun sequence.
ACCESSION   DAOSOX010000236 DAOSOX010000000
VERSION     DAOSOX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29131_length_3294_cov_8.241741"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="cyaB"
                     /locus_tag="PLX22_11660"
     CDS             complement(<1..393)
                     /gene="cyaB"
                     /locus_tag="PLX22_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOT20594.1"
                     /translation="MAYRLPVHYEDIKHNNAMLNTMLEVEIKSKCDNLDEVKKKIVDC
                     GGTLLSFGIEDDSYFIHPSRDFAKTDEALRIRKVNDAYYFTYKGPRLGGKSKTRYEKE
                     MKITDADALQEILLRLGFQLYGRVQKKRE"
     gene            complement(356..1102)
                     /locus_tag="PLX22_11665"
     CDS             complement(356..1102)
                     /locus_tag="PLX22_11665"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOT20595.1"
                     /translation="MATKKKGKSSATKKASGSKKVKRGSSSNAPYILVIMALAAGLIF
                     LLSLQSDSLKNIFDKKNNRKSITKVEVPPDDRKLGKKDSNATMNKAGDTIKEKPHTQQ
                     LSPKEKEKAVTVSAKVYFLYYNEKTDHIELMPVVRMVNAHTPVKSALEELAKGPTANE
                     EKKGLVNAVPSTLKVIDVSIINNIAFINFNSALEEGAAGNILMNRLDQIVYTATQFDN
                     VEGIHILVNGQRKRFLGSDGISVAGPLRRH"
     gene            1327..2748
                     /locus_tag="PLX22_11670"
     CDS             1327..2748
                     /locus_tag="PLX22_11670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT20596.1"
                     /translation="MAQPSNTLEQIIENIHAGDDSTRNKAFEDIIECGSEAIVPLITL
                     LGHPDEQMRDSAVWALCQMNDVAGPILIQELLNKNPLIRESASFCMGICKNKDASQAL
                     LLLLKDENEKVRETAAWSLVKIGVDDENTVKQIIACLDDESVLVKDTAQWILEHIGES
                     AFEPLFQKLMESSSNSERIIHILSIISSDKIDALLQLLETTQDIQKLSYLIQLIGQLR
                     DPQSIECLLPFLNHSNRHIITHTVEALINIGELTIEPLFEKLEQGSISPTNEMMPLFI
                     RHKSASVPQLIAMLQSSNNSLRIFAARALGVIEDKRAIEPCITGLKDSNIYFVEACAR
                     ALSAFTNKLIIKPLTDCIMDSNYPIVKSNAIDALAGVLDSKAALQLLQKFNEASLTVK
                     KRLLQLASYYPSSEAEELLQQALLIDDDECKMIALQGLRKIGTEKSIECVRDAIIKEN
                     PYISDEARSALKEIIERKQREIK"
     gene            2824..3027
                     /locus_tag="PLX22_11675"
     CDS             2824..3027
                     /locus_tag="PLX22_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20597.1"
                     /translation="MAMNIKEVILYDADSLEYSGKILVDGNNWQFSEVSNNFLVTFTK
                     GMQLKAMLQCLISFNIVYDIIES"
     gene            3038..>3294
                     /locus_tag="PLX22_11680"
     CDS             3038..>3294
                     /locus_tag="PLX22_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT20598.1"
                     /translation="MDNHLSHETILTIIKFQKSEITEYHIYNILSSHIKNHHNARILK
                     KIADDEKKHYDFWKGYTHSEVKPNRFKIAKYYIISRLFGLT"
BASE COUNT         1078 a          643 c          539 g         1034 t
ORIGIN      
        1 ttccctcttt ttttgcactc gcccgtataa ttgaaatcca agccgcagca gtatctcctg
       61 caatgcatcg gcatcagtta ttttcatctc tttttcataa cgtgttttgc tttttcctcc
      121 cagcctgggt cccttatagg taaaataata tgcatcattt accttccgta ttctcaacgc
      181 ctcatcggtt tttgcaaaat cacgagatgg atgaataaaa tagctgtcat cttcaatgcc
      241 aaaagataac aatgttccac cacaatcaac tatctttttt tttacttcat caaggttgtc
      301 acattttgat tttatttcta cttctaacat agtatttaac atagcattat tgtgtttaat
      361 gtcttcgtaa tggaccggca accgatatgc catcactgcc cagaaaacgt ttacgctgtc
      421 cattaaccaa tatatgaatt ccttcaacat tatcaaactg cgttgctgtg tacacaatct
      481 gatcaagacg attcatcaaa atatttcctg ctgcaccctc ttccaatgca ctgttgaaat
      541 taataaaagc aatattgtta attattgaaa catcaataac cttgagtgtt gatggaacgg
      601 catttaccag cccctttttc tcttcatttg cggttggacc ttttgccagt tcttcaagtg
      661 cactcttcac cggagtatgg gcatttacca tacgcacaac aggcatcaac tcaatatggt
      721 cagtcttttc attgtaatac aaaaaataaa cttttgctga tacggtaact gctttttcct
      781 tttcttttgg cgatagttgt tgtgtatgag gcttttcttt tatagtatcg ccggctttat
      841 tcattgttgc attgctgtct ttttttccca gcttcctgtc atcaggaggc acttcaactt
      901 ttgttatgct tttacggtta ttctttttat caaaaatatt ttttagtgaa tccgattgca
      961 aagaaagtaa aaatataagc cctgcagcta aagccataat gacaaggata tagggagcat
     1021 tagacgatga gccgcgttta accttcttag aacctgatgc cttttttgtt gcagaagatt
     1081 ttcccttttt tttagttgcc attatccttc cctaaaaata caatcaaaat gcaattatag
     1141 aaatatcatc atacagtatg ctaatttaga tattcatcgc atgctggaat tactagatgt
     1201 aataaaccat actcataaca aatattgcaa ctaattattt tgaaattgta gtatttttag
     1261 tatatattgc aaacataata atgctaagta tttttttaat caatcatatt ctataatagt
     1321 acaataatgg ctcaaccatc aaacactctg gaacagatta tcgagaacat ccatgcaggt
     1381 gatgatagta cccgcaataa agcatttgaa gatataattg aatgcgggag tgaagcgata
     1441 gttcctttaa ttacattgct tggtcatccc gatgaacaga tgcgcgacag tgctgtatgg
     1501 gcgttgtgcc agatgaacga tgtagccggg ccaatcctta tacaggaact attaaacaaa
     1561 aatcctttaa tacgcgaatc agccagcttc tgcatgggta tatgcaaaaa taaagatgca
     1621 tcacaagcac tgctgttgtt attaaaagac gagaatgaaa aagtgcgtga aacagcggca
     1681 tggtcgctgg tgaagattgg ggttgatgac gaaaatactg taaagcaaat tattgcatgc
     1741 cttgatgatg aaagtgtact ggtaaaagac acagcacagt ggatattaga gcacattggc
     1801 gaatcagcat ttgaaccttt gttccaaaaa cttatggaat catcatccaa tagcgagcgg
     1861 atcattcata tcctttccat aatatcatct gacaaaattg atgccttgct gcaattgctg
     1921 gaaaccacac aggacattca aaaactatca tacttaattc aactaatagg acaactgcgt
     1981 gatccacaat ctatagagtg ccttttaccg tttctcaatc atagcaacag gcatatcata
     2041 acacacaccg ttgaagccct cattaacatt ggcgaattaa ccatagaacc tctatttgaa
     2101 aagttggaac agggtagcat ttccccaacc aatgagatga tgcccctttt tatacggcat
     2161 aaaagcgcat cggtccctca acttattgcc atgctacaat ccagcaataa tagtttacgc
     2221 atctttgccg cccgtgcttt aggtgtcatc gaagataaaa gagcaattga gccatgcatc
     2281 actggactaa aagacagcaa catatatttt gtagaagcat gtgcccgtgc attgagcgct
     2341 tttaccaata agcttatcat taaaccatta accgattgta ttatggatag caactatcct
     2401 attgttaaat ccaatgccat tgatgcatta gccggtgtcc ttgacagtaa agcagcattg
     2461 caattattgc aaaaatttaa tgaagcatct cttacagtta aaaaacgatt actgcaactt
     2521 gcatcatact atccatcctc tgaagctgaa gaacttttac agcaagccct gcttatagat
     2581 gatgacgaat gtaaaatgat agcccttcag ggattacgaa aaataggcac tgaaaaatct
     2641 attgaatgtg tacgggatgc tatcatcaaa gaaaatccat atataagtga tgaagcccgc
     2701 tcagcgttaa aagaaataat tgaaagaaaa caacgcgaaa taaaataaaa aacttgataa
     2761 attttacatt tatactttaa ctgtataaat catcattata ttcaaatatc aggagttatg
     2821 actatggcaa tgaatataaa agaagtaatt ttatatgacg ctgattcatt agaatactct
     2881 ggtaaaattc tggtagatgg caataactgg cagttttcag aagtttcaaa taattttctg
     2941 gttaccttta caaaaggcat gcagttaaaa gccatgctcc agtgtcttat atcgttcaat
     3001 attgtgtatg atattattga atcgtaaggt aaccataatg gataatcacc tatcacatga
     3061 aaccatactc actataataa aatttcagaa aagtgaaata accgaatacc atatctataa
     3121 cattctgtca tcacatatta aaaatcacca taatgctcgt atcttaaaaa agatagcgga
     3181 tgatgaaaaa aaacattatg atttctggaa aggctatacc cactctgaag taaaacccaa
     3241 tcgctttaag attgcaaaat attatattat ttctcgctta tttggactta cctt
//
LOCUS       DAOSOX010000237         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29332_length_3276_cov_5.951568, whole genome shotgun sequence.
ACCESSION   DAOSOX010000237 DAOSOX010000000
VERSION     DAOSOX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29332_length_3276_cov_5.951568"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1391
                     /locus_tag="PLX22_11685"
     CDS             12..1391
                     /locus_tag="PLX22_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT20599.1"
                     /translation="MATHLTLDQRSPGSSPGRADVHYKDTSIISHISYNFPSISIAMY
                     TVALIIFIITYSGIMVTRLPGINIDRPSAAYFGAIAMIAAGVLSFEEAIAAIDFNTIA
                     LLLGMMIVIATLELDGFFAFVAHKTIAFSKTPSQLLWIITFTTGIASAFLVNDAVVLM
                     FTPVIISICRHAQLDPKPYLIAEILASNIGSSMTITGNPQNMLIGIYSKIPYIYFILH
                     LFPIAILGMIIIIIVIKRIYSQTFEAPSLLHYDENEENEYDYNLSSMIFSVPIFLIMT
                     ILFLFHHMLHLSIPLIALIGSSFILLLGKIKPSKIIKEIDWVLLLFFASLFIVVKGAE
                     KAGILNIVMHYSQLNNTFYSTIVLHGISLISSQIISNVPFVILLVPILKQNNSKLLWL
                     ALASSSTLAGNATIIGAMANLIVLESAARAGIKISFIEFLKPGLMVTIITLLMSIVLL
                     YLQLNFQLL"
     gene            1716..2057
                     /locus_tag="PLX22_11690"
     CDS             1716..2057
                     /locus_tag="PLX22_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT20600.1"
                     /translation="MQIDVHKEGPLLILTLNGRYDISKIHNFETIYRQNLEDNPKIIA
                     LNLRDLKYIDSSGIGSLVRCMNLALKNNIEFICYDINENVESIFKIAKLDQFISILTH
                     DEFIKKYIKKQ"
     gene            complement(2044..3036)
                     /locus_tag="PLX22_11695"
     CDS             complement(2044..3036)
                     /locus_tag="PLX22_11695"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT20601.1"
                     /translation="MAIASFLQYTTMKMKKVTVLLYCILLSAIVTITVLFLLFNHFIL
                     NQYHLSLLLKSVFFIAVIIVIFNISFIIIAKVLHKQYYGVARSMLKVLKQYEKGNYTY
                     LFSIDNNGFDSILKYMQKLLAGSNNPEVSLDKQLDEKVFAIISVLISAMEDKDPYTSG
                     HTARVAQYSLAIGKHLGLPKEKMRLLNHAAAAHDIGKIGIPIEIIEKNGKLTDREKLL
                     LQSHPIRGMKILSTLDYLKDIATIVMYHHERIDGQGYYELSGNKIPVEAKIIAVADAL
                     DAMLSDRPYRKGLSLHEAKQELEKNKGTQFDPLIVDALIQLLEEKGTLKELIAF"
BASE COUNT         1085 a          564 c          487 g         1140 t
ORIGIN      
        1 cggtggtgta attggcaaca catctgactc tggatcagag aagtccaggt tcgagccctg
       61 gccgggcaga tgtccattat aaagatactt caattataag tcatataagt tacaatttcc
      121 ccagcatatc aattgccatg tatactgttg ctcttattat cttcattatt acatattcag
      181 ggataatggt aacccgcctg ccaggcatta acattgacag gccatcagca gcttattttg
      241 gagcaattgc aatgattgct gcaggtgttt tatcttttga agaggcaatt gccgctatag
      301 atttcaatac aatagcactg cttttaggaa tgatgattgt tattgcaact cttgaactgg
      361 atggattttt tgcttttgtt gctcataaaa caattgcttt ttccaaaacg ccatcacaat
      421 tattgtggat aataacattt actaccggta ttgccagtgc atttctggtt aatgacgctg
      481 ttgttttaat gtttacccct gttattatct caatatgccg ccatgcacaa ttagatccta
      541 aaccttattt aatagctgaa attttagcat ctaacattgg aagttcaatg accattaccg
      601 gtaacccgca aaatatgctg attggtattt attcaaagat tccctatata tattttatac
      661 tgcatctgtt ccctattgct atactcggca tgataattat tattattgtt attaaacgga
      721 tatattcgca aacctttgaa gcaccatcac tattacatta cgatgaaaat gaagaaaatg
      781 aatatgatta caaccttagt tctatgatct tttctgtacc aatttttttg attatgacta
      841 tacttttcct ttttcatcat atgcttcatt tgtcaatacc tcttattgct ctgattggaa
      901 gttcttttat tcttttatta gggaaaataa agccttcaaa aattataaaa gaaattgatt
      961 gggtgttact gttatttttt gcttcactat ttattgtcgt aaaaggtgct gaaaaagcag
     1021 ggatattgaa tatagtgatg cattactcac agcttaataa tactttttat agtactattg
     1081 tattacatgg aataagtctt atctcttccc agataatcag caatgtgccc tttgtcattc
     1141 ttctcgttcc aatactgaaa caaaacaaca gcaaactgct atggctggca ttggcttcat
     1201 catcaaccct ggcaggtaat gccacaataa ttggtgccat ggctaatctt attgttctgg
     1261 aatcagcagc aagagcaggt atcaaaataa gctttataga atttttaaaa cctggattaa
     1321 tggttacaat aataaccttg ttaatgtcaa ttgtgctatt atatcttcaa ctgaactttc
     1381 agttgctata aatttcatag tatcaactac cacgtttata tcatacagga aaatcaaaac
     1441 aaaattgtaa atacccgtgt gattacaaaa tatttgataa gctgcttttt atcttttgct
     1501 ttcaagcata taaaaaaatg aaaaaaacac tattgatagc tacctataaa tatatgcaaa
     1561 ggtaagtggt acataattat tttaattatt tgttgacatc tttcaataaa taaattattc
     1621 ttatgaataa ttatacaata atttgttatt acctttcaca acacattatt gtatatcagg
     1681 atacactatt aatattttaa ttgatgagta attgcatgca aattgatgtt cataaagaag
     1741 gtccactatt aatacttacc ttaaacggac gctatgatat ctcaaagatt cataattttg
     1801 aaactatata ccgacaaaat ttagaagata atccaaaaat cattgcactt aatttacggg
     1861 atctaaaata tattgactca tccggtattg gttcattggt acgctgtatg aaccttgcat
     1921 tgaaaaacaa tattgaattt atttgttatg acatcaatga aaatgttgaa agcattttta
     1981 aaatagcaaa acttgatcaa tttatatcca tattaaccca tgacgagttt ataaaaaaat
     2041 atattaaaaa gcaataagtt cttttagtgt gcctttttct tctaatagtt gtattagtgc
     2101 atcaactata agcgggtcaa attgcgtgcc tttatttttt tccaactctt gtttagcttc
     2161 atggagcgat agtccctttc tatatggtct gtcgctcaac atggcatcaa gggcatcggc
     2221 aacagcaatg atcttagctt caacagggat tttattgcct gacaattcat aatatccctg
     2281 cccatcaatt cgttcatgat ggtacatcac aattgttgca atatccttta aataatcaag
     2341 cgttgataat attttcatgc cgcgtatggg atgactttgt aaaagcaact tttccctatc
     2401 agttaatttc ccattcttct cgataatttc aattggtatg ccaatttttc caatatcatg
     2461 cgcagcggca gcatgattga gcaatcgcat cttttctttt ggtaagccaa gatgtttacc
     2521 aattgcaagc gagtactgtg ccacccgtgc agtatgaccg ctggtatagg gatctttatc
     2581 ttccatggcg cttatgagca cactaatgat tgcaaatacc ttttcatcta attgtttatc
     2641 aagtgatact tctggattat ttgatccggc taacaatttc tgcatatatt ttaaaatact
     2701 atcgaaacca ttattatcta tggaaaataa ataggtataa ttgccctttt catactgttt
     2761 cagaaccttc agcatacttc gagcaacacc gtaatattgt ttatgcaaaa cttttgctat
     2821 aataataaat gatatgttga aaataacaat aataaccgca atgaagaata cacttttaag
     2881 caataatgac agatgatact gatttaaaat aaaatgatta aataacagga aaagaacagt
     2941 tatagtaact atcgcagaca gtaatatgca atagagtaac actgttacct ttttcatttt
     3001 catagtagta tattgtaaaa aacttgcaat agccatacat tacacctcat tactcaccat
     3061 acgtaaaata atcgtttttg atgaaataaa ccaaaaatat aatatttttt ataagcaggt
     3121 tattaaaatt aatattgacg atataatgca gacagttaca tctgtttata tcaacattgc
     3181 atgcaataca caaaattaac catacttaaa aaaggtagca aaaatgtcaa ggatactttc
     3241 aggaatacaa ccatcgggta cattgcatat aggtaa
//
LOCUS       DAOSOX010000238         3266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29438_length_3266_cov_7.194021, whole genome shotgun sequence.
ACCESSION   DAOSOX010000238 DAOSOX010000000
VERSION     DAOSOX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29438_length_3266_cov_7.194021"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..>3266
                     /locus_tag="PLX22_11700"
     CDS             222..>3266
                     /locus_tag="PLX22_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT20602.1"
                     /translation="MNNNKSIFLGIDAGSTALSIVAIDSGKNIVDTVYTFHHGKIHEA
                     IANYCAQFGKTDVCGIGFTSSAAAITGGHEVNSIVAYVTAIKHTLPQIRSCVIVGGEK
                     FGVIEFDDKGNYSQYRGNTSCAAGTGGFLDQQARRLQLDGSHELSAMALKNDGDIPKI
                     ASRCAVFAKTDLIHVQQEGYDITEICDGLCYGVARNIADTLFASHTPAQPVAIVGGVS
                     LNSAVVKHLESIVGVRFHVPELSHIYGAFGAALYVANNTLQNAVEFTFNTTLKNKLYH
                     FPPLAITMSNYPTFNGIESFVHTSLHDVEVDIYKPMNGFVETFIGIDIGSTSTKMALI
                     DKQGTMLAGFYTRTAGKPLEAVQSIFEACDVYAAKHGISIFVKGAATTGSGRKFIGTL
                     IGADCILDEITAHAKAACTLQPDVDTIIEIGGQDSKFTMIKNGNVIFSQMNNVCAAGT
                     GSFIEEQAYKLGCNLAEYATRALNKQSPLSSDRCTVFMERDINYCMSQGYDTDELLAA
                     VLHSVVENYLSKVSGEAKIGNVICFQGATAKNKALVAAFEQKLQKPLHVSQYCHLTGA
                     LGCALEVMELDITQSSFRGIGLYKKSIPVKSEICNLCTNHCKLKVADVDGEVVAYGFL
                     CGRDYHYKKYVSKTKGIFDFSKERNKIVTLSSNEDTAQVGIPYALHLVDEMDLWIDFF
                     KRLNVTVITSSGCDDPVTIGKRIAQAEFCAPIAAYHGHVTYLADRCNYIFTPFYLEKD
                     APSHSRRQYCYYTQFAPSLTSLIPSVSKKVIMPILNYEIQPLNSRRLLYKACKQMGIN
                     ANSIEVMMAFEQAQNTHKENQKKLMKSGKHAIETRKDIAVVLLGRPYNCIPDSMNKNI
                     PSLFVNLGIPVLYQDMIQYNDDDSHAIDELLKAFHWHYAARILQIAHGIAKSDGLYPV
                     LLTSFKCAPDSFVMEYFKRILDEAGKPYLILQLDEHDSTVGYETRIEAAVRSFKNHYT
                     KRLVKTTAIPVVPSVTRETLKGKTVLLPNWDTMASRLLKANLM"
BASE COUNT          993 a          565 c          728 g          980 t
ORIGIN      
        1 ggcgtaccgc aagcgttatt ctattgcaca gtgggtataa aaaaggggtc agagctaaag
       61 aaaaggaatt tataaattac tgtcactatt tataaaaatt atattttttt attgattatt
      121 tatgctattt atgtatacta tccagaataa acaaaattat gtatgtgtaa ataaatagta
      181 ctgtaatata ctatcaattt actatgtgac agggtttggc tatgaataat aataaatcta
      241 tttttttagg tattgatgct ggctctacgg cgctttccat tgttgctatt gacagcggaa
      301 aaaatatcgt tgatacagtg tatacatttc atcatggaaa aattcatgaa gcaattgcaa
      361 actattgtgc ccaatttggt aaaaccgatg tttgtggtat aggttttact tcttcggcag
      421 ctgctataac gggaggtcac gaggttaata gcattgttgc atacgttaca gctattaaac
      481 atactttgcc gcagatacgt agctgtgtta ttgttggtgg tgaaaagttt ggtgttattg
      541 agtttgatga caaaggcaat tattcacagt atcgtggcaa tacaagctgt gcagcaggaa
      601 ccggaggatt tttagatcaa caggctcgaa gattgcagct tgatggttca catgaattgt
      661 ctgccatggc tttaaaaaat gacggtgata tccctaagat tgcatcgcgg tgtgccgtat
      721 ttgccaagac cgaccttatt catgtacaac aggaaggata tgatatcacc gaaatatgtg
      781 atggactatg ttatggtgtt gcgcgcaata ttgctgatac cctgtttgca tcgcatacac
      841 ctgcgcagcc ggttgccatc gtgggtggcg tgtcgttaaa cagtgcagtt gttaaacacc
      901 ttgaatcgat tgtaggtgta aggttccatg taccggaact atcgcacata tatggcgctt
      961 ttggtgctgc attatatgtg gcaaataata ctttacaaaa tgcggttgaa tttaccttca
     1021 atacaacatt aaaaaataag ctgtatcatt tcccaccatt agcaatcaca atgagcaatt
     1081 atcccacatt taacgggatt gaatcgtttg tgcatacatc cctgcatgat gttgaggtgg
     1141 atatctataa acctatgaat ggcttcgttg aaacatttat tggcattgat attgggtcca
     1201 ccagcacaaa gatggcactg attgataaac aggggactat gctggcaggt ttttatacgc
     1261 gtactgcggg aaaaccgctt gaggcggtgc aatcaatttt tgaagcgtgt gatgtatatg
     1321 ctgcaaaaca tggtataagc atttttgtta aaggggcggc aacaacaggt tcgggacgaa
     1381 agttcattgg aacactcatt ggggcagatt gtatccttga tgagattact gcacatgcaa
     1441 aggcagcatg cacattgcag cccgatgttg atacaatcat tgagattggc ggacaggatt
     1501 caaagtttac catgataaaa aatggcaatg ttattttttc gcagatgaat aatgtgtgtg
     1561 cggctggcac tggcagtttc attgaggagc aggcatacaa gctgggatgt aaccttgctg
     1621 aatatgctac cagagcgcta aataaacagt cgccgctttc cagtgaccgc tgcacagtat
     1681 ttatggagcg ggatatcaat tattgcatga gtcagggtta tgatacagat gagctgttag
     1741 cggcggtatt gcattcagtg gtggaaaatt atctttctaa agtttccggc gaggctaaga
     1801 ttggcaatgt tatctgtttt cagggagcaa ctgcaaaaaa taaagcgctg gttgctgctt
     1861 ttgaacaaaa gctgcaaaag cctttgcacg tttcacagta ctgtcatctg accggtgcgc
     1921 ttggttgtgc gttagaggta atggaattgg atattacaca atcaagtttt agaggtatag
     1981 ggctgtataa aaaatcaatt ccggtaaaaa gtgaaatttg caatctgtgt accaaccatt
     2041 gcaagttaaa agttgctgat gttgatggcg aggttgttgc gtatggattt ttatgcggtc
     2101 gcgattatca ttacaaaaag tatgtcagta aaacaaaggg tatctttgat tttagcaagg
     2161 aacgcaataa gattgttaca ttatcttcaa atgaagatac tgcacaggtg ggtattccgt
     2221 atgcattgca ccttgttgat gaaatggatt tatggataga cttttttaaa cggttgaatg
     2281 ttacggttat taccagcagt ggatgtgatg acccggtaac tatcggcaaa aggattgcac
     2341 aggctgaatt ttgcgcacca attgctgcat accatggaca tgtgacatat ctggcagata
     2401 gatgtaatta tatatttacg cctttttatt tagaaaaaga tgctccttcc cattcaagga
     2461 gacagtattg ctactataca cagtttgcgc catcattgac ctcgttaata ccatcggtta
     2521 gcaaaaaagt tatcatgcct atacttaact atgaaataca accattgaac agcagacgat
     2581 tgctatacaa agcctgcaaa cagatgggaa ttaatgctaa ctctattgaa gttatgatgg
     2641 catttgaaca ggcacagaat acccataaag aaaatcagaa aaaattgatg aaaagtggaa
     2701 aacatgccat tgaaaccaga aaagacattg cagttgtatt gcttggcagg ccatataatt
     2761 gtatccccga ttccatgaat aaaaatatac cgtcattgtt tgttaattta ggcattcccg
     2821 tcctctatca ggatatgata cagtataacg atgatgatag ccatgcaatt gatgaactgc
     2881 tcaaagcatt tcactggcac tatgctgcca ggattttgca gatagctcat ggtatagcaa
     2941 aatctgatgg attatatcca gtattgctga catcatttaa atgcgccccc gattcatttg
     3001 ttatggagta cttcaagcgc attcttgatg aggcgggcaa accgtatctt atattgcagc
     3061 ttgatgagca tgattcaacg gtgggatatg aaacgcgcat tgaagctgct gtcaggtcat
     3121 ttaaaaatca ttatacgaaa aggctggtaa aaacaactgc tattccggta gttccttcag
     3181 ttacccgtga aacactcaaa ggtaaaacgg tactgttgcc aaactgggat acaatggcat
     3241 cccgattgct gaaagcaaat cttatg
//
LOCUS       DAOSOX010000239         3249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29641_length_3249_cov_9.887602, whole genome shotgun sequence.
ACCESSION   DAOSOX010000239 DAOSOX010000000
VERSION     DAOSOX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29641_length_3249_cov_9.887602"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PLX22_11705"
     CDS             <1..1196
                     /locus_tag="PLX22_11705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOT20603.1"
                     /translation="YGEVTMRECVFIDGVRTPNGRAHREKGWFRHLRPDELLTPVYNA
                     LFERNSKITPDMLDAVFVGCANPSGMQNDIGRLAWLASGFPDSVPTNTLTNQCPSGMS
                     ATMHAARAIMTGEADIIIAAGVEDMEKVPMGANMDFPPRLLKYYNGADLLMGPTAEKV
                     AELWKIAREDMENMAIWSNKNAWEATVAGKFRKEIVPIKGLDDNGNEFTVDRDQWIRE
                     KIEPEKMKTMMSPFKPNGVVTAATSSPLTQGACALLLMSREKADALGLSYKYKYSYGV
                     LAGCDPTIMGIGPIPAIKKLLSRTGLNLDDVGAIELNEAFASQSLACIRELKLDNENA
                     PFKRVNVWGGALALGHPLGESGARIIVTLLNVLATEFPAAKYGLASLCGAFGNAGALL
                     VEKVN"
     gene            1241..1882
                     /locus_tag="PLX22_11710"
     CDS             1241..1882
                     /locus_tag="PLX22_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HOT20604.1"
                     /translation="MKKSFTFLFTVLFITITVFVFSQQQWKLIYEGKEDNAEIKIYTR
                     ELPGSIYKEFKGVSYADIPFNVALKVIQDYTNYHKWYGMCDALYAITVRNQQDLDMYF
                     VLDLPVVKNRDVVVKVESGWDENKGTAWVTMTSINGSEYKKDSGYVRITSLKGRWDII
                     KKDENHVEVIYQVHPELGGSLPAWIVNVAAKDQPLKTLKGVYKYSKKIIDKND"
     gene            2010..2399
                     /locus_tag="PLX22_11715"
     CDS             2010..2399
                     /locus_tag="PLX22_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20605.1"
                     /translation="MLMYYILTFGCVAFAIGLLEIIIPQVMMSLLIKWTSSKLYSIHG
                     IILIAAGFPLTQIPSSNPLSIPLFIVGLIIVFTGPFILIYPEKVKKLFLEGLDELGHE
                     GRHLALTIDALFQMIVGIVCAVAFFLQ"
     gene            complement(2407..>3249)
                     /locus_tag="PLX22_11720"
     CDS             complement(2407..>3249)
                     /locus_tag="PLX22_11720"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT20606.1"
                     /translation="LYARFFTMVLNDLGLLQYREPFTRLLTQGMVIKDGAKMSKSKGN
                     IVDPDDSIDKYGADTVRLFMLFAAPPDKDLDWSDKGVEGCYRFINRVWRIVQKYRDCY
                     TTYNFDTIVLDSSLKNLRNEIHRTVKAVTHDIEDRMQFNTAIARMMELVNALYLADET
                     LIQTDRGKMVVSELFDKLLPMLGPFIPHVAEELWELLGHKDFIVNAAWPDYIEELTMK
                     EEIELVFQINGKIRSKCNVAANITREEMEKAALEDDRIKEMIAGKTVKKVIVVPGKLV
                     NIVI"
BASE COUNT         1031 a          582 c          612 g         1024 t
ORIGIN      
        1 tttatggaga ggttaccatg agagaatgtg ttttcattga tggtgtaaga acaccaaatg
       61 gaagggctca tcgagagaaa ggttggttta gacatttaag gcctgatgag cttttaacac
      121 ctgtttataa tgcattattt gaacgcaata gcaaaattac tcccgatatg cttgatgcag
      181 tttttgtagg atgtgctaat ccttctggaa tgcaaaatga tattgggaga ttagcatggc
      241 tggcatctgg atttcctgat tcagtaccaa ccaacaccct taccaatcaa tgtccatcag
      301 ggatgtcggc aaccatgcat gctgcacgag caattatgac cggtgaagct gacattatca
      361 tagcagcagg agtggaggat atggaaaagg ttcccatggg agccaatatg gactttccac
      421 cacgactttt aaaatactac aacggtgcag atcttttgat gggccccacc gctgaaaaag
      481 ttgctgaact gtggaaaatt gcacgtgaag atatggaaaa tatggcgata tggtcaaaca
      541 aaaatgcatg ggaagcaact gtcgctggta aatttagaaa ggagatagta cctataaaag
      601 gtttggatga taatggtaat gagtttacag ttgataggga tcaatggatt cgcgaaaaaa
      661 tagaaccaga aaagatgaaa acaatgatgt ctccattcaa accgaatggt gttgtgacag
      721 cagcaacatc atcaccatta actcaaggtg catgcgcact tcttctcatg agtcgcgaga
      781 aggctgacgc ccttggttta tcatataagt acaaatattc ttatggagtt ttagctggtt
      841 gtgatccaac cattatgggt attggtccca taccagccat taagaagctt ctctcacgga
      901 caggattaaa tttagatgat gttggtgcta ttgaacttaa tgaggcattt gcaagccaat
      961 cattagcatg catccgggag ctcaaacttg ataatgagaa tgcaccgttt aaacgtgtta
     1021 atgtgtgggg aggagcactt gcattaggtc atcctcttgg ggaatctgga gcacgcatta
     1081 ttgtaacact acttaatgtt ttagcaactg aattccctgc tgcaaaatat gggcttgctt
     1141 cattatgtgg agcatttggt aatgcaggtg cattactggt agaaaaggtt aattaattat
     1201 aatagtattt cataaatata ttaaaccatg gaggtttgct atgaaaaaat cgtttacctt
     1261 tttattcaca gtcctattta tcactataac agtttttgtt tttagccaac aacaatggaa
     1321 attgatatac gaaggtaaag aagacaatgc tgaaatcaaa atttacacac gggaattacc
     1381 tggttcaatc tataaagaat ttaaaggagt tagctatgca gacataccat ttaatgttgc
     1441 tttaaaagtg attcaggatt atacaaatta tcataaatgg tatggtatgt gcgatgcttt
     1501 atatgcaatc actgtacgga atcaacagga tttagacatg tactttgtac tggaccttcc
     1561 tgttgtaaaa aatcgggatg tagtagttaa ggttgaatct ggctgggatg aaaataaagg
     1621 aactgcatgg gtaaccatga catctattaa tgggagtgaa tataaaaagg acagtggcta
     1681 tgttagaata acttctttaa agggtcgatg ggatataatc aaaaaagatg aaaaccacgt
     1741 tgaggtgata tatcaggtac atccagaatt aggcggctca ctcccggcat ggatagtaaa
     1801 tgttgctgca aaagatcaac cgttaaaaac actgaaaggt gtatataaat attcaaaaaa
     1861 aattatagat aaaaatgact gatacagtaa ataaaccata agaggagctt ttaagcctct
     1921 tatggtttat taattctccc aaatttcttg acatatttat gattccaaat gttaatcttc
     1981 ctatatattg tatttattaa ggatagacca tgttaatgta ttatatttta acatttggtt
     2041 gtgttgcatt tgctataggg ttattggaaa ttattatccc acaagttatg atgtcattat
     2101 tgattaaatg gacttcaagc aagttatatt ctatccatgg catcatactt attgcagcag
     2161 gatttccact tacacaaata ccatcgtcaa atccactttc aatacctctg ttcattgttg
     2221 ggttgattat agtattcacc ggaccattta tattaatata cccggaaaaa gtaaaaaaac
     2281 ttttccttga aggccttgat gaattaggac acgagggcag acaccttgca cttactatcg
     2341 atgcactatt ccagatgatt gttggaatag tttgtgcagt agcttttttt ttacaataaa
     2401 tctgacttat ataacaatat ttaccagttt accaggaact actataacct ttttaacagt
     2461 tttccctgca atcatttctt ttatcctatc atcttccagc gctgcttttt ccatttcttc
     2521 tcttgttatg tttgcagcaa cattacactt tgaccgtatc ttcccattaa tttgaaaaac
     2581 taattctatc tcttctttca tagttaattc ttcaatatag tctggccatg ctgcattgac
     2641 aataaaatcc ttatgcccta atagctccca taactcctcc gcaacatgag gtataaatgg
     2701 tcctaacatg ggtaaaagct tgtcaaaaag ttcggataca accattttgc ccctgtcagt
     2761 ttgtatcaga gtttcatctg ccagatagag tgcattgaca agttccatca ttcgtgcgat
     2821 agctgtatta aattgcattc tatcttcaat atcatgggta acagctttaa ctgttctgtg
     2881 gatttcattc cttaagtttt tcagagaact atctaacacg atagtatcaa aattatatgt
     2941 ggtataacaa tctctatact tctgtactat acgccacact ctgttaataa atctgtagca
     3001 accttccact cctttatctg accagtccag atctttatcg ggtggagcag caaaaagcat
     3061 gaacaaacga actgtatcgg caccatactt atcaatacta tcatctggat ccacaatatt
     3121 cccttttgat ttactcatct tagcgccatc tttaatgacc attccctgtg tcaacaatcg
     3181 tgtaaatggt tcgcgatact gtaataatcc taaatcattc aaaaccatgg taaagaatcg
     3241 tgcatacag
//
LOCUS       DAOSOX010000240         3241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29735_length_3241_cov_9.639674, whole genome shotgun sequence.
ACCESSION   DAOSOX010000240 DAOSOX010000000
VERSION     DAOSOX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29735_length_3241_cov_9.639674"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLX22_11725"
     CDS             complement(<1..917)
                     /locus_tag="PLX22_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20607.1"
                     /translation="MKKLIVLFIVFIFPAYAFAQRQVDKKPVVHTVTIEFNGETIKGF
                     YCFIDARSNEEKSSNVENKALHGSVILFLQGHAQRADDAYKFTSQMALTSKSGVVIVV
                     VCDTPYGKDPQYCGDAGKEIIVMAMMQYALTLQGINVIDAEQMPEAITINGKTVSISQ
                     SGIPATLTVVGWSHGALLARRVANRYNCVKNLVQICPAGFNDWPDNSCAANCCVLSAF
                     SWESLRISTEMFKGHANDVFAASWGITKGIAGDSCRSCGSCLHGNFTVCKSLRAVKDI
                     TDVTLQANADNFPVTTLSSIVVIFGSNDSL"
     gene            complement(979..1647)
                     /locus_tag="PLX22_11730"
     CDS             complement(979..1647)
                     /locus_tag="PLX22_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOT20608.1"
                     /translation="MRLENKKIIVSGGPTREWIDPVRYISNASSGKMGVAIADAAYNR
                     CINTVFIHGPVEHSIIANKPYQCIGVETTVDMLEAIQQELSPNTILIMAAAPADYAPV
                     HKSPVKIKKNNDTLTIALKRNPDILKTIATLRKENKQLRDVTLIGFAAETTNIMQYGK
                     TKLVEKELAMICINDVSSTDSGFGSDYNEVTIMLANEETMHLHKMTKKEVAENILQII
                     ETMV"
     gene            complement(1631..2167)
                     /locus_tag="PLX22_11735"
     CDS             complement(1631..2167)
                     /locus_tag="PLX22_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOT20609.1"
                     /translation="MKSKIILGISSSIAAYKSCDLARMLTRDGYDVFCVLTHNATHLV
                     TPLTLQTLTGNPVYTTSFALEWREMGHIELKDNAQLLLVAPATANIIGKFANGIADDL
                     LSTTFLSVNCPVVIAPAMNPNMWNHKAVQENITKLKKWGAIIVEPDTGLVACGDEGQG
                     RLASIESIYNIAINAIRK"
     gene            complement(2164..>3241)
                     /locus_tag="PLX22_11740"
     CDS             complement(2164..>3241)
                     /locus_tag="PLX22_11740"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20610.1"
                     /translation="NLIDGSLQDLECTITGYISKPEYYRSNRSLQFLYVNKRPVQFPY
                     FSFILQQVYQTILQKGQYPVAFICMDVKPDLIDVNVHPAKREIRFFDNNYIHSMLYNF
                     ANKIISGGIHTIAIGDYERRYAIATNHHEATDTNDNEMVSEVTPPLQYTESGNKNAFL
                     KEIRNVYTQMQMKDYAVLGVIFGEFIVIQKHDELYFIDFHAAHERKLYDYLLQVNTTV
                     AVQQLLIPVVIEFSPDMVMLVNSYRDVLLDYGLLLEQFDDNSVVVNAIPDFCTTSDIE
                     GLIKDVVDSLYENKNHIDNIKMTIIEKIACHAAKRAHDSITDADIIAITDYVFTCSDY
                     RCPHGRPFVYKLSKSELEGYFKRL"
BASE COUNT          992 a          677 c          544 g         1028 t
ORIGIN      
        1 agtgaatcat tgctgccaaa tataacaact atcgatgata gagtagttac cgggaaatta
       61 tctgcgtttg cttgtagtgt tacatcggtg atatctttta ctgctcgtaa tgacttacat
      121 accgtaaaat taccatgcag gcacgaacca catgaacgac aactatcgcc cgctatccct
      181 tttgtaatac cccagcttgc cgcaaataca tcgtttgcat gccctttaaa catttcggtt
      241 gatatacgca ggctttccca tgaaaatgct gataatacac agcaattggc ggcacagcta
      301 ttatcaggcc agtcattgaa accagccgga catatctgta caagattttt gacacagtta
      361 tatctgtttg ccacacgccg ggctaacaat gcaccatgcg accacccaac caccgtaagc
      421 gttgcaggta tgccactttg cgatatcgat accgttttgc catttattgt tatagcttct
      481 ggcatctgct cagcatcaat gacattaata ccctgcaagg ttaaagcata ctgcatcatt
      541 gccataacaa taatctcttt acctgcatca ccgcagtatt gagggtcttt accgtagggc
      601 gtatcacata ccacaacaat cacaacacct gattttgagg ttaatgccat ctgcgatgta
      661 aatttatatg catcatctgc tcgctgggca tgcccctgca aaaacaaaat aactgaccca
      721 tgtaatgcct tgttttctac attagaactt ttttcttcat tgctacgagc atctatgaaa
      781 caataaaagc ccttgattgt ctcaccatta aattcaatgg taacggtatg cacaactggc
      841 tttttatcca cctgacgctg tgcaaatgca taagctggaa aaataaatac aataaacaaa
      901 acaattaatt ttttcatgca tagtcactgt gtaataaatt aatacaataa accacaatca
      961 atatttgcat tctttaattt ataccatagt ttcaataatt tgtagtatat tttccgcaac
     1021 ttcttttttt gtcattttat gcagatgcat tgtttcttca tttgcaagca taatggttac
     1081 ttcattatag tcgctcccaa accctgaatc ggtactgctt acatcattga tacatatcat
     1141 ggctaattct ttttcaacaa gttttgtttt gccatattgc ataatattgg ttgtttctgc
     1201 tgcaaaccct atcagtgtta catcacgcaa ctgtttattt tcctttcgca aggtagcgat
     1261 agtttttaaa atatcgggat tacgctttaa tgcaatggtt aaggtatcat tatttttttt
     1321 tattttcaca ggcgatttat gaacaggagc ataatctgcc ggtgcagcag ccataattag
     1381 aatagtattg ggcgatagtt cctgttgaat agcttcaagc atatcaacgg tagtttccac
     1441 gccaatacat tgatagggtt tattagctat gatagaatgt tcaaccgggc catggatgaa
     1501 aactgtattg atacacctat tgtacgcagc atctgcgata gcaaccccca tctttccgct
     1561 tgatgcgttg gagatatagc gaacagggtc aatccactcg cgcgttggcc cgccggaaac
     1621 aattattttt ttattttcta atcgcattga ttgcaatgtt ataaatggat tcaatagatg
     1681 ctaatctacc ctgcccttca tcaccgcagg caaccaatcc tgtgtccggt tcaacaatga
     1741 tagcgcccca ttttttaagc tttgtaatat tttcctgtac tgctttatgg ttccacatgt
     1801 tgggattcat ggcaggagct atcaccaccg ggcaattgac cgataaaaat gttgtgctaa
     1861 gaagatcatc cgcaatgcca ttggcaaatt taccaattat attggcagta gcaggtgcaa
     1921 ccaataataa ctgcgcattg tcttttaatt caatatgacc catttcacgc cactcaagtg
     1981 caaagctggt agtatacact ggattgccgg ttaatgtttg cagcgtcaat ggtgttacaa
     2041 gatgcgtggc attgtgtgtt aaaacgcaaa agacatcata accatctctg gtaagcatac
     2101 gtgctaaatc acatgattta tatgcagcaa tagacgagga tatacccaat ataattttgg
     2161 atttcataac cgtttaaaat acccctctaa ttctgatttt gacagcttat acacaaatgg
     2221 cctgccatga ggacatcggt agtcactgca tgtaaaaaca taatctgtaa tggcaataat
     2281 gtctgcatcg gttatactat catgtgctct ttttgccgca tgacaagcaa ttttttcaat
     2341 tatagtcatt tttatattat caatatgatt tttattttca tataaactat caacaacgtc
     2401 ttttatcaat ccttcgatat cactggttgt acaaaaatca ggaatagcat tgacaacaac
     2461 tgaattatcg tcaaattgtt ccagcaacaa accatagtca agcaaaacat cacggtaact
     2521 attgacaagc ataaccatgt cgggtgaaaa ctcaataaca accggtatca ataattgctg
     2581 aacagcaact gtcgtattta cctgtaaaag ataatcgtat agtttacgtt catgcgcagc
     2641 atgaaaatca ataaaataga gctcatcatg cttttgtatt acaataaatt caccaaaaat
     2701 aacccccagt acagcatagt ctttcatctg catttgagta taaacattac gtatctcttt
     2761 caaaaaagca tttttattac ctgattccgt atactgtaat ggcggcgtta cttcggatac
     2821 catttcatta tcgttagtat cagtagcttc atggtgatta gttgctatag cataccgcct
     2881 ttcatagtca ccaattgcaa tggtatggat accgccgctg ataatcttat tggcaaagtt
     2941 atacagcatg gaatgaatat aattgttatc aaaaaatcgt atctctcgtt ttgcaggatg
     3001 cacattaaca tcaattaaat caggtttaac atccatacat ataaaagcaa ccggatactg
     3061 tcccttttgc aatattgtct ggtaaacctg ttgtaaaata aaactgaagt atggaaactg
     3121 caccggccgt ttattgacat acaaaaactg taatgacctg ttagaacggt agtactcagg
     3181 tttagaaatg tatcctgtta tagtacattc taaatcctgt aatgaaccat caattaaatt
     3241 t
//
LOCUS       DAOSOX010000241         3235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29808_length_3235_cov_10.237421, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000241 DAOSOX010000000
VERSION     DAOSOX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29808_length_3235_cov_10.237421"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLX22_11745"
     CDS             <1..1221
                     /locus_tag="PLX22_11745"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20611.1"
                     /translation="ITEAVDEGYNTDVVFVPISINYDRILEESSYVQELKGKEKEKES
                     VVSLMQSSKLLNRKYGMVYINFNKTFTLKELKEKLNPVNITEAVAMNIVRRINDVVTI
                     TPFSLTTTAILMSSVKGFSRDMVKANFESLYDYCEYKNYSMSESLKDKSYFDEIIDYV
                     IQSFKEDKILETLKIVGEGKKLEAMPDMYVLPEENRPRMIFYKNSIVHYFLPLTFSSL
                     ALRVLHKNGQSSFDSFRSEYVYIRDLFMKEFIYSEDMLEDVMQSIQSVVTYMQMTGFA
                     TVAQNEITINADREKDAKLRFFGRILQEYLESYYVVCATLLTATHTRIQKKDLLTEIR
                     KNGVRMYHTGEIMLSESLSLPNYTNALSLLRSEKALSEKQMGRKNIVIEIINQKTLEI
                     LFEKIKIYLEGIRA"
     gene            complement(1411..1980)
                     /locus_tag="PLX22_11750"
     CDS             complement(1411..1980)
                     /locus_tag="PLX22_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOT20612.1"
                     /translation="MKLSNKLACLVVAMVLVPSVSFAIVDFGAYGGYTFAGKIDSPTI
                     DDAETDGWQYGFIGHLNKSIIPMILSMGIGGFYQRSPLEYSDSGKDYDLTKTMYGIDA
                     IATLELPIIHPYLRAGIAINEKIKVDTPSGTYTDEKKFNSYYFGLGAAFTVFPFIQIF
                     GEYLYNYSKQEHDSTLHSNSINIGARLNI"
     gene            2460..>3235
                     /locus_tag="PLX22_11755"
     CDS             2460..>3235
                     /locus_tag="PLX22_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20613.1"
                     /translation="MSQKSKDIVHELEEIADIPETIIDISEEDIVIDLPEEEKPSGEF
                     EEITDLTDSTSSTITIEDENPSLQLHEMDDDVINITLNVDAIHEVRVNNRSSKPAISN
                     PVQKAKLFEDIPEPMESFINGTSSLNVKEEYVHTAYDDENREVKGKIYFDNAADALVI
                     EDIVEEDEKLSQKSESQKLSQKTIEQNKNIPTDVEQHASSAMNDDKESQKGTSLRSDV
                     ITIEIPPEVRTKIKKSKIEDFDFINLDEAEQIASEEILILS"
BASE COUNT         1200 a          460 c          553 g         1022 t
ORIGIN      
        1 attaccgaag ctgttgatga agggtataat accgatgtgg tttttgttcc tatttcaatt
       61 aattatgacc gtattcttga agagtcaagc tatgttcagg aacttaaagg aaaagaaaaa
      121 gaaaaagaat cagtggtaag cctgatgcaa agcagtaaat tgcttaaccg taaatatggt
      181 atggtgtata taaattttaa taaaacattt actttaaagg aacttaaaga aaagcttaac
      241 cctgtcaata ttactgaagc agttgccatg aatattgtcc gaagaattaa tgatgttgta
      301 actatcacac cgttttcatt aacaactact gccatcctca tgtcatcggt gaaaggattt
      361 tcacgtgata tggttaaagc taactttgag tcattatacg attactgtga atataaaaat
      421 tattcaatgt cagagtcatt aaaagataaa tcatattttg atgagatcat tgattatgtt
      481 attcaatcgt ttaaagaaga caaaatttta gagacattaa aaatagttgg agaaggtaaa
      541 aagttagaag ccatgcctga tatgtatgtg ctcccagaag aaaacaggcc aagaatgatt
      601 ttttacaaaa attcaattgt tcattacttt ttgccactga cattttcatc attagctttg
      661 cgggttttac ataaaaatgg gcaatcttct tttgattcat ttagaagcga atatgtatat
      721 attcgtgatc tgttcatgaa agaattcatt tattcagagg atatgcttga ggatgttatg
      781 caatcaatac aaagtgttgt tacatacatg cagatgactg gttttgctac agtcgctcag
      841 aatgaaataa caattaatgc ggatcgtgaa aaggatgcaa agcttagatt ttttggaaga
      901 atattgcagg aatatttaga gtcatactat gttgtatgcg caacattact tactgcaacg
      961 catactcgga ttcaaaaaaa agatttgcta actgagatac gcaaaaatgg tgtcaggatg
     1021 tatcatacag gcgagataat gctttccgag tcactatcgc tccctaatta taccaatgca
     1081 ttgagtttat tgcgatctga aaaagcatta tcagagaagc aaatgggaag aaaaaatatt
     1141 gttatagaaa ttataaatca aaaaacatta gagattctct ttgaaaaaat aaaaatttat
     1201 ctggaaggaa ttcgcgcata aaacaaggct gattatttta atgatagtaa tagaggcagt
     1261 tttaaacaaa agactataaa aaaataaaca cgcctctatt ttttttgcta ccgtaacaaa
     1321 tacttttttt atgtaattaa caattgagta tacaatgtta gcactatttg ttattaatca
     1381 cacagttgtt gaaataaagc tatagttgtg ttatatgttt aagcgcgctc caatatttat
     1441 ggaattactg tgtaatgttg aatcgtgttc ctgctttgaa taattataaa gatattcccc
     1501 aaaaatctgt ataaatggaa atactgtaaa agcggcccct aatccaaaat aatatgaatt
     1561 aaactttttt tcatcggtat aagtgcccga tggtgtatca accttaatct tttcattaat
     1621 ggctatgcct gcacgcaggt aaggatgaat aataggaagc tcaagcgttg caattgcatc
     1681 tatgccatac atggtttttg ttagatcata atctttaccg ctatctgaat attccagtgg
     1741 ggaccgttga taaaaaccac caatacccat actcagtatc attgggatta ttgatttgtt
     1801 aagatgtcca ataaaaccat actgccagcc atcagtttca gcatcatcaa tggtggggct
     1861 atctatttta ccagcaaagg tatatccacc atatgcgcca aaatcaacta tcgcaaagga
     1921 tactgatggt acaagcacca ttgctactac taaacatgct aatttgtttg ataacttcat
     1981 aataagtatc tctccttgct ttttaaaata ttaaaaatat tagtgatagt tatcaatatt
     2041 aatgacacaa tacctataat aggatatacg atatcattaa ttattgaata ccattggaaa
     2101 ctatcatagt ggttaataat acattggcta tacaacaaag tgcaatgatt tttcatgagt
     2161 aatgaaaaaa ttttatataa tcatgtatat agtattataa tgtaatgatt tgaatgtcaa
     2221 aatatttaca tggtacacgc tgttttatgg tatttaataa tattacatgg ttaaccgata
     2281 ttgtacagta gggtagtttt ataattattt ataaattgtg tttgattata tatctgagag
     2341 tttgtactac gttattgtat tatttgtggt aagcaataga aattttaata aaaattgtat
     2401 atattgatac tataaggaaa tctaaagttt aaaatttaaa agaaaagaat gcaaatggta
     2461 tgagccagaa aagcaaagat attgttcatg aacttgaaga aatcgctgat attcctgaaa
     2521 ccattattga catctcagag gaggatatcg ttattgattt acctgaagaa gaaaaacctt
     2581 caggcgaatt tgaagaaata actgatttaa cggatagtac atcctccacc attactattg
     2641 aggatgaaaa tccttctctc caactacatg agatggatga tgatgttatt aatattacgt
     2701 tgaatgtaga tgccatacat gaagtaaggg ttaacaatag aagcagtaaa cctgctatca
     2761 gcaatcctgt gcaaaaagca aagttatttg aagacatacc cgaaccaatg gaatctttta
     2821 taaacggaac atcttcatta aatgttaaag aagaatatgt tcatactgcc tatgatgacg
     2881 aaaataggga agttaaagga aaaatttatt ttgataatgc tgctgacgca ttggttattg
     2941 aagatatagt ggaagaagat gaaaaattat cccaaaaaag tgaatcccaa aaactatcgc
     3001 aaaaaacaat tgagcaaaac aaaaacatac cgacagatgt tgaacaacat gcgtcatcag
     3061 caatgaacga tgataaagaa agtcaaaaag gtaccagttt acgttctgat gtaataacaa
     3121 tagaaatacc tccagaagtg agaacaaaga ttaaaaaatc aaaaattgag gattttgatt
     3181 ttataaatct ggatgaagct gaacaaatag ccagtgaaga aatccttatt ctttc
//
LOCUS       DAOSOX010000242         3227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_29882_length_3227_cov_9.859079, whole genome shotgun sequence.
ACCESSION   DAOSOX010000242 DAOSOX010000000
VERSION     DAOSOX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29882_length_3227_cov_9.859079"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..754)
                     /locus_tag="PLX22_11760"
     CDS             complement(5..754)
                     /locus_tag="PLX22_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT20614.1"
                     /translation="MVSSQEIEAQCILHINNFEGPLDLLWELIKKSKIEITQVSIAEI
                     TDQYCNYLSLMEKINIPIAMDFIVMASELIYYKSKALLPGADIGDEFFVPPLPPELIQ
                     KLLEFKKYQLASKNLISLHAMQSDVFTRQPNLVNNDEVVYLEVNLFDLLNAFVDVLES
                     EKEVESQSIVFDEILVSDRIILLISMLQDKEQIEFNELFPQKPGRMEVVVTLLAVLEM
                     IRIGAIAVLQNAQFGNMYIFKKSLEKAELIY"
     gene            complement(900..1259)
                     /locus_tag="PLX22_11765"
     CDS             complement(900..1259)
                     /locus_tag="PLX22_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT20615.1"
                     /translation="MAKILIVDDAKFMRTLVKDALVPRGHEIIGEAENGVEAVQLYKK
                     LRPDLVTMDITMREKDGIEAAEEILLFDPNARIIMVTALGQENLLTRAIKIGVKDFVV
                     KPFPPERLQKAADKALA"
     gene            complement(1442..2494)
                     /locus_tag="PLX22_11770"
     CDS             complement(1442..2494)
                     /locus_tag="PLX22_11770"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOT20616.1"
                     /translation="MSMSQKIKVMVVDDSALVRKVITDILESDKDIEVIGTANNGKSA
                     VFKNLTLNPDVITMDIEMPIMDGLEALQEIIKTNPKPVIMMSVLTQYGAEATFRALEF
                     GAIDFIPKPSNLLAMSMDDIAELLISKVKAVARVSLKISHSKDIMNIQSTKLTVDSNQ
                     KRVKKIVGIGTSTGGPSALQRVLQKLPGNFPSPILVVQHMPPGFTKAFAERLDSLCHV
                     SVKEAEDSEEAVDGCVYIAPGDSHIVMEKMNNKRIIRLSQSEKVSGHRPSADVLFQSM
                     VKADLENNIGVIMTGMGRDGAMGIKEIHNNGGYTIAQDESTSVVYGMNRVAVEMGAVD
                     AVVDLDSIPDEIVARI"
     gene            complement(2494..>3227)
                     /locus_tag="PLX22_11775"
     CDS             complement(2494..>3227)
                     /locus_tag="PLX22_11775"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOT20617.1"
                     /translation="GESEKSQNNNIIEKQVHNALEEFKSELKEKIKTTLGEENEIYKK
                     LGISDDDLAKVQVLANVGITDAAEKLSKILGRRVDLSIPDVSVLAIETIPQFFGDIDS
                     VYLGVYMPIVGQINGTILFSLHQESGYELVDMLLGLPQGTTTSLSEDGESVLKEVTNI
                     VGSAVVNAFADKIEMAIKPTVPSVVHDYMQSILDSILAFHTAENEYALVMDTEFFHQD
                     DRVIGKLMIMPTTSSLSTIVERLRN"
BASE COUNT         1000 a          690 c          450 g         1087 t
ORIGIN      
        1 tacctcaata tattaattca gctttttcca acgatttttt aaaaatatac atattgccaa
       61 attgtgcatt ttgtaatact gctattgcac caatacggat catttccaat accgccagca
      121 acgtaactac cacttccatc ctgccaggtt tttgtggaaa tagctcatta aactcaatct
      181 gctctttatc ctgcagcatg cttataagta gtataatacg atcactaaca agaatttcat
      241 caaatactat actctggctc tcaacctctt tttcagattc aaggacatca acaaaggcat
      301 tgagaagatc aaaaaggtta acttctaaat atacaacttc atcattgtta acaagattgg
      361 gctgccttgt aaatacatct gattgcattg catgtaatga aatcaaattc tttgaagcaa
      421 gctgatattt cttaaattca agtaattttt gtattaattc agggggcaaa ggtggaacaa
      481 aaaattcatc tccaatatca gcaccaggta atagtgcttt tgatttgtag taaataagct
      541 cgcttgccat aacaataaaa tccatagcaa ttggaatatt tatcttctcc ataagggaca
      601 gatagttaca atactgatca gtaatttcag ctatggaaac ctgcgttatt tctattttag
      661 actttttaat aagctcccat agtaaatcca gtggaccttc aaaattattt atatgcaaaa
      721 tacattgcgc ttcaatttcc tgtgatgaaa ccatgattta accacaatta caaatagttt
      781 ttttaattat aaaaaaaaga ctattaatga caatcttttt atttatgcaa cgataattaa
      841 tttttattat ttaaataata caagtttgca ataattcctt aattcattct acgatgtatt
      901 tatgctaatg ccttatctgc agctttttga agtctttcgg gagggaatgg tttcactaca
      961 aaatctttaa ctccaatttt tattgctctg gttaataaat tttcctgacc caaagcggtt
     1021 accattataa tacgtgcatt gggatcaaac agtaaaattt cctcagctgc ttcaatacca
     1081 tctttttcac gcattgtaat atccatggta accagatctg gccgtaattt tttataaagc
     1141 tgaacagctt caacaccatt ttcagcctca ccaataattt catgccctct tgggaccaac
     1201 gcatctttaa caagggttct cataaatttt gcatcatcta ctattagtat tttagccata
     1261 gccatacccc aatgtatata atatcccaca tccacacacc agtatataat cttaaataca
     1321 tattaccgat aagcctattt cattcaagaa aaatttttat taattacaat tttttactaa
     1381 tactcctgca ttgaatactc ttataaactt taaccgccta ataacagcta taacaagcaa
     1441 cctatatgcg agctactatc tcatcaggta ttgaatcaag atcaacaact gcatcaactg
     1501 cccccatttc taccgccacc ctattcatac catataccac tgacgttgat tcatcctgtg
     1561 caatagtata accaccatta ttatgaattt ctttaatacc catagctcca tctcgtccca
     1621 ttccggtcat tatcacccct atattatttt ccagatctgc ttttaccatc gattgaaaca
     1681 agacgtccgc tgatggtctg tgacccgata ccttttcaga ctgtgacaac cgtatgatac
     1741 gtttattgtt cattttttcc atcacaatat gagaatctcc cggcgcaata tatacacatc
     1801 catcaacagc ttcttcacta tcttcagctt ccttaacaga aacatgacat agactatcca
     1861 atcgttcggc aaatgccttt gtaaatcccg gaggcatatg ttgtactacc agtattggtg
     1921 aaggaaagtt tccaggcaat ttttgcaata cccgttgcaa tgccgatggc ccaccagttg
     1981 atgtaccaat cccaacaatt ttttttaccc ttttttgatt actatcaacc gtcagctttg
     2041 ttgattgtat attcattata tctttgctat gagatatctt cagggatacg cgcgccacag
     2101 cctttacttt cgatataagt aattctgcaa tatcatccat gctcattgcc agaagatttg
     2161 aaggtttagg aataaaatca attgccccaa attcaagtgc tctaaacgtt gcttcagcac
     2221 catattgcgt tagcacactc atcataatga caggttttgg attcgtcttt atgatttcct
     2281 gcaatgcttc aagcccatcc attatgggca tctctatatc cattgtaatt acatcaggat
     2341 taagagtcag atttttaaat actgcagatt tgccattatt tgcagtgcca ataacctcta
     2401 tatctttgtc actttcaaga atatcagtta tgacttttct gacaagagca gaatcgtcaa
     2461 caaccatgac cttaattttt tgtgacatag agattaattt cgcaaccttt caactatcgt
     2521 acttaatgat gaagttgttg gcattatcat aagtttacca atcactctgt catcctgatg
     2581 gaaaaattca gtatccatca ccagggcata ttcattttcc gctgtatgaa aagccagaat
     2641 ggaatcaagt attgactgca tgtaatcatg aacaactgat ggaacagttg gctttattgc
     2701 catttctatt ttatcagcaa atgcattaac aacagccgaa ccaacaatat tggtaacctc
     2761 tttaaggact gattcaccat cttcgcttaa tgatgtggta gtaccctgag gcaacccaag
     2821 cagcatatca acaagttcat aaccggattc ctggtgcaaa ctgaaaagga tggtaccatt
     2881 aatctgtcca acaattggca tataaacacc aagataaaca ctgtcaatat caccaaagaa
     2941 ttgagggatt gtttctatcg ccagtactga tacatccggt atggacagat caactcttct
     3001 gcccagtatt tttgaaagtt tttctgcagc atcagtaatc ccaacatttg caagaacctg
     3061 aacctttgca agatcatcat ctgatatacc taatttttta taaatttcgt tttcttcacc
     3121 aagggttgtt tttatttttt ccttcagctc cgatttaaac tcttcaaggg cattatgaac
     3181 ctgtttttca attatattgt tgttttgact tttttctgat tccccta
//
LOCUS       DAOSOX010000243         3197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_30216_length_3197_cov_7.849141, whole genome shotgun sequence.
ACCESSION   DAOSOX010000243 DAOSOX010000000
VERSION     DAOSOX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30216_length_3197_cov_7.849141"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLX22_11780"
     CDS             complement(<1..572)
                     /locus_tag="PLX22_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOT20618.1"
                     /translation="MEHNTGNIILYDTTLRDGAQTFGISFSLQDKIRIALELDALKID
                     YIEGGWPGSNPKDNLFFSEIRSASINHAKIAAFGSTKRPKMKLAEDELLQNLIQSNAH
                     TLTIVAKSWDFHVTDALNITLEENLNMVYDTIAYCKDKGFEVFLDAEHFYDGFKANGD
                     YAMKVLESAKKAGVDMLVLCDTNGGTLTHE"
     gene            853..1986
                     /locus_tag="PLX22_11785"
     CDS             853..1986
                     /locus_tag="PLX22_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20619.1"
                     /translation="MKQSITYNAKQKRWGLLLYGVLALLIITLCIVMYVFIYYSINHS
                     LLQPSYLFLFVLFVLMLVTGIYSTIKFLVITTNEIVSSKNELALKDVIIEHSNEIIIL
                     LDNIGKILSINPALTTILNFEKEELIQQPFRAVLYEKSFEDSVRLRDLLLSRFKDVFK
                     GYEAEIVLPCKVQHYAEIKSIAFKLVPIFKDEQLQYILAIGRIIQSDYLTSNYLKSEF
                     AEFILDNNIRMVNMLSYRLTRNLETYMEKKEIIRIQLGLQEAIINAIEHGNLEIDFKT
                     KSLLKKKNGNYWDHVIDRCNKEYLEKRKIKVQYYLSPDHVKYIITDEGKGFEWEHYIT
                     TLPDGNLINNYHGVGLTMLQDIFNVSFNEKGNEITMIKYFNKG"
     gene            1990..2412
                     /locus_tag="PLX22_11790"
     CDS             1990..2412
                     /locus_tag="PLX22_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT20620.1"
                     /translation="MTAYRNPVPTVDIIIQLEDEYGNVKPDEIVLIKRKNPPYGWALP
                     GGFVDYGETLEQAAIREAKEETSLDVILLRQFHTYSDPARDPRQHTITTVYIARAKGK
                     PEANDDAREVKIFNICNVPATMAFDHAQILEDYKNNRY"
     gene            2384..>3197
                     /locus_tag="PLX22_11795"
     CDS             2384..>3197
                     /locus_tag="PLX22_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOT20621.1"
                     /translation="MKTIKITDIEEVAMDISFNLMKLTGLAIDTLSKLSKATIRVHDP
                     ERIPKAPVVFVVNHFTRMETFFLPNVIYKITGKYLYSLAHYSFFTGSFGRFMEKVGAV
                     STKDPMRDVKFIGALLRGDMDCIIFPEGQMIKDKRFIERGKYMVYNSGIRRPPHTGAA
                     NIALHAQFYREELKSCQIRNACDELNTLMAHFGIHDNELSHVVDQETYIVPVNITYFP
                     IRAHRNIINKIASTFVKSLPERIEEELEVEGTMLVEGVDIDINFGNPIAISPY"
BASE COUNT         1066 a          504 c          614 g         1013 t
ORIGIN      
        1 atttcatgag tcagagtgcc gccgttagtg tcgcatagta ccagcatatc aacaccagct
       61 ttttttgcac tttctaaaac cttcatagca taatcgccgt tggctttaaa accatcataa
      121 aaatgctcgg catctaaaaa gacctcaaat cccttatcct tgcaataggc aatggtatca
      181 tacaccatgt ttaaattttc ttccagcgtt atgttgagtg cgtcagtaac atgaaaatcc
      241 catgattttg ctacaatggt cagggtatga gcattggatt ggataagatt ttgtaaaagt
      301 tcatcttcgg ctaatttcat tttaggacgt ttggtggaac caaaggctgc aattttagca
      361 tggtttatag aagcgctacg gatttcacta aagaaaagat tatcctttgg attggatcct
      421 ggccagccgc cttcgatata gtcaatcttc aatgcatcta attccagtgc aatacgtatt
      481 ttatcctgaa gggaaaacga aataccaaac gtttgggctc catcgcgtaa tgtagtatca
      541 tataaaatga tattccctgt gttatgttcc ataatagctc acgagtatta taactgttta
      601 ccatggtgtt agcatatcat taaatatata acactttttc tggtagtaca aatattatta
      661 aataatttta atggagtgtt atattttttt tgcattgaat gacaatataa acgataatgc
      721 atataatggg aactgtgtaa aatattcaag ctattttttt gacaaaatgg acaatttgct
      781 tgaaattaat aattaatatt gattatagtg gttactatag taaataatta atatcgtttg
      841 ccataatgaa gtatgaaaca gagtataaca tataatgcta aacaaaagcg atggggtttg
      901 cttctttatg gagttttggc tctattaatt attaccttat gtatagtaat gtatgtattt
      961 atatattact ccatcaatca ttcattgtta caaccttctt atttatttct ttttgtttta
     1021 tttgttctga tgttagttac agggatctat tcaactataa aatttttggt tataacaaca
     1081 aatgagattg ttagcagtaa aaatgaatta gcattaaagg acgtcattat agagcattcc
     1141 aatgaaatta tcatactcct tgataatatt ggaaaaattt taagcattaa tcctgcatta
     1201 acaaccatac ttaattttga aaaagaagaa ttgatacagc aaccattcag agcagtttta
     1261 tatgaaaagt catttgaaga ttcggtgaga ttacgggatc ttttgcttag cagatttaaa
     1321 gatgtattta aaggatatga agctgaaatt gttttaccgt gtaaggtgca acattatgcc
     1381 gaaataaaat ctattgcatt taagttagtt cccattttta aagatgaaca gttgcaatat
     1441 atccttgcta taggaaggat tatacaatcc gactacctta ccagtaacta tctcaaaagc
     1501 gaatttgcag aatttattct ggacaacaac atacgcatgg taaacatgct gtcatataga
     1561 ttaacaagaa atttagaaac ctatatggaa aaaaaagaga tcatacgcat acagttgggg
     1621 ctgcaggaag ctatcatcaa tgccatagag catggtaatt tagagataga ttttaagaca
     1681 aagagccttt taaagaaaaa gaatggcaat tattgggatc atgttattga tagatgcaat
     1741 aaagagtatt tagagaagcg gaagattaag gtacagtatt atttatcccc tgatcatgtg
     1801 aaatacatca taactgatga gggtaaaggt tttgaatggg agcactatat tacaacgctg
     1861 cccgacggta atttaatcaa caattatcat ggtgtggggt taacgatgtt gcaggatatc
     1921 tttaatgtgt cgtttaatga gaaaggtaat gagataacca tgataaagta ttttaacaaa
     1981 gggtgacata tgactgctta ccgcaaccct gtacctaccg ttgacattat tatacagctt
     2041 gaagatgaat atggcaatgt taaacctgat gagattgtgt taataaaacg taaaaatcct
     2101 ccttatgggt gggctttacc aggtggcttt gtggattatg gtgaaacgct ggagcaggca
     2161 gcaatacggg aggcaaagga agagacatcg cttgatgtta tattgctgag gcaatttcac
     2221 acctattctg atccagcacg ggatccgcgt cagcatacta ttaccactgt ctatattgcc
     2281 agggctaaag gaaaaccaga agccaatgat gatgccagag aagtaaaaat ttttaatata
     2341 tgcaatgtgc ccgccaccat ggcttttgat catgcacaga tacttgaaga ctataaaaat
     2401 aaccgatatt gaggaagtag caatggacat ttcgtttaat cttatgaaat taacagggtt
     2461 ggcaattgat acgctttcta aattaagcaa agctactata cgggtacatg atcccgaaag
     2521 aattcctaaa gctcctgttg tatttgtggt aaaccatttt acccgaatgg aaacattttt
     2581 tcttcccaat gttatttata aaataacagg aaagtatctc tattcattag cacattacag
     2641 cttttttaca ggcagctttg gaagattcat ggaaaaggtt ggtgctgttt caacgaaaga
     2701 tcccatgaga gatgttaagt tcattggtgc tttgctcaga ggggatatgg attgtataat
     2761 attccctgaa gggcagatga taaaggataa aaggtttata gaacgtggca aatatatggt
     2821 ttacaactcc gggatacgac ggccacctca taccggtgct gccaatattg cattacacgc
     2881 acaattttat agagaagagt tgaaaagctg ccaaattcgt aatgcatgtg atgagttgaa
     2941 cacattaatg gctcattttg gtatacatga taatgaatta tcacatgttg ttgatcagga
     3001 aacttatatt gtaccggtaa atataacata ttttcccata cgagcgcatc gaaatattat
     3061 caataagata gccagcacat ttgttaaaag tttgcccgag cgcatagaag aggagcttga
     3121 ggttgaaggg acaatgctgg tagaaggtgt tgatatcgat atcaattttg gcaatcccat
     3181 tgccataagc ccctatt
//
LOCUS       DAOSOX010000244         3196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_30232_length_3196_cov_4.778733, whole genome shotgun sequence.
ACCESSION   DAOSOX010000244 DAOSOX010000000
VERSION     DAOSOX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30232_length_3196_cov_4.778733"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PLX22_11800"
     CDS             complement(<1..1375)
                     /locus_tag="PLX22_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT20622.1"
                     /translation="MKKCMVLTCMFFIFTVSLYAQDITIIQTTDLHSHFLGFSPNIDY
                     TPATINDDNTVGGWARIATVINDTKKSRKNPVLVLDSGDFLMGSLFHLLCREESFELR
                     LMKTMGYDCITLGNHEFDLFPDGLARIIQSAKRYNALPAIVASNVVFSAESKDDDSLE
                     NVFEQELVKTYTVLQKGRLKIGLFGIIGKDAAEVSPFADPVTFADQVKTATTMVDILK
                     NKEKVDVVICLSHSGIKYYKNKKEIDPDRSEDVQLAKVVNGIDVIISGHTHTKITRPL
                     IVNKTIITQSYEYGKQVAVLDCTVSKDGVSLKDYKYIDINDSIKGDAAITAMIQQFTQ
                     VINQRVLSPMKLQWNSILAKTTFDLTLKEDECNLGNLIADSIRWYVNKVDANPDDPDS
                     KVAMTVISGGVIRDNILVGKTGKVALCDAFAAIPLGIGMDDTMGYPLITCYLYASEVK
                     KALEILTS"
     gene            1826..2740
                     /gene="dusB"
                     /locus_tag="PLX22_11805"
     CDS             1826..2740
                     /gene="dusB"
                     /locus_tag="PLX22_11805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT20623.1"
                     /translation="MNTTFPIHVKSKLFLAPMAGYTDLPFRLIAHTSGAGMVFSELLS
                     ADGIVRSNKKTLAMLSFSDEEKPLGIQIFGNDPAVMAEAAVRLLSHKPDCIDINAGCC
                     APKVCNNGKGAGLLNNVELLHRIAYAVVQSVDIPVSAKIRLGFTQGTKNYREVVAALE
                     QAGVSFITVHGRTRDQFYSGKADWDAIGTIADFSKVPIVGNGDIASYEDAMEKLSTSG
                     CAAVMIGRAAIGNPWIFCGQQPSYQEKIAMIKKHYECMVQHYGNYGIILMRKHIVHYL
                     KGFSGAVKLRQKLLTLTSMDEILEVLHY"
     gene            complement(2729..2953)
                     /locus_tag="PLX22_11810"
     CDS             complement(2729..2953)
                     /locus_tag="PLX22_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOT20624.1"
                     /translation="MNWKDRIIIDPNICHGKPCIKGTRIIVSVILDNLANGMKEEDIV
                     RSYPSLTSEDIQAAIAYAAELSHERLILIM"
BASE COUNT          935 a          677 c          589 g          995 t
ORIGIN      
        1 tactggttaa aatttccagc gcctttttaa cctcagaggc atacagatag caggtgatca
       61 aaggatagcc catggtgtca tccataccaa tgccaagcgg gattgctgca aaggcatcac
      121 acaatgcaac cttccccgtt ttgccaacca gaatattatc ccggataacc ccgcctgata
      181 taacagtcat tgctactttg ctgtcaggat catcgggatt ggcgtcaacc ttattaacat
      241 accatcgtat ggaatcggca atcagattgc caaggttgca ctcatcctct ttcaaggtaa
      301 gatcaaatgt tgttttagca agtatactgt tccattgtaa tttcatgggc gatagtaccc
      361 gttgattgat tacctgtgta aattgctgaa tcatcgcggt aatggcagca tcacctttta
      421 tactatcgtt tatatcaata tacttatagt cttttaacga taccccatct tttgagacag
      481 tgcaatccag cacagcaacc tgcttgccgt attcatatga ttgtgtaatg atggttttat
      541 tgacaataag cggccgggta atctttgtgt gagtatgacc gctgataata acatcaatac
      601 cattgactac ctttgccaat tgcacatcct cagagcggtc aggatctatt tcttttttat
      661 ttttataata ctttataccg ctgtgcgaaa ggcatataac aacatccact ttttctttat
      721 tttttaagat gtcaaccatg gtggttgctg tcttcacctg atcggcaaat gttaccggat
      781 cagcaaaagg agaaacctcg gcagcatctt tgccaataat accaaacaat cctattttta
      841 atcttccttt ttgcaaaact gtatatgtct taacgagctc ctgttcaaag acattttcaa
      901 ggctgtcatc gtctttgctt tccgcactga agacaacatt tgaagcaact atcgccggca
      961 gagcattgta ccgttttgct gattgtataa tgcgagcaag cccatccgga aatagatcaa
     1021 attcatgatt gcctaatgtg atgcaatcat atcccattgt tttcatcaaa cgcaattcaa
     1081 acgattcttc cctgcaaaga agatggaaca atgaacccat caaaaaatca cctgaatcaa
     1141 gtaccagcac aggatttttc ctgctctttt tggtatcgtt tattacggtg gctatgcgtg
     1201 cccagccacc aacagtattg tcatcattaa tggttgcagg tgtataatca atattgggcg
     1261 agaaacctaa aaaatgcgaa tgtaaatcgg ttgtctgtat aattgtaata tcctgtgcat
     1321 acagtgatac agtaaaaata aagaacatgc acgttagtac catacatttt ttcataacag
     1381 tcaaaccccc tatcattatc tttcattgct gtttgtacat gcatcgtact gaattatcaa
     1441 gcataaaatt tttaaatttt ttccacctgt actaaaacat agcattattc caacctgtca
     1501 ttttgggctt gaccttgaat ccattaaaat cattgtcatt ctgagtgaac gcagtgcttt
     1561 ctgtcattct gagggagcgc cagcgaccga agaatctatc cttattgtac aatgagattc
     1621 tttacttcgc tcagaatgcc ccccttgtgt cccccccccc ttataaaaag cggggaattt
     1681 tagtcagagt tttgacgaca gatagatttt tttacttcgt tcaggatgac atgccactcc
     1741 attcttatct gcggttgaca agtttttgct aaaccaatac agattatttt tttattgata
     1801 gcattcaaag agctattatc gttgtatgaa cactacattt cccatacatg taaaaagtaa
     1861 actttttctt gctcccatgg ccgggtacac tgatctgcca ttcaggctga tagcccatac
     1921 atctggtgca gggatggtat tctcagagct tttaagtgcg gatggtatcg ttcgcagcaa
     1981 taaaaaaact cttgccatgc tgtcgttttc tgatgaggaa aaaccgctgg gaattcaaat
     2041 ttttggcaat gaccctgctg tcatggcaga agccgctgtc aggttactat cgcacaagcc
     2101 tgattgtatt gatatcaatg ctggctgttg cgctccaaaa gtatgcaaca acgggaaagg
     2161 tgctggttta ctcaacaacg ttgaactgct acacaggata gcatacgcgg tggtgcaaag
     2221 cgttgacata ccggtttcag ccaaaatacg gcttggcttt acgcagggca ccaaaaatta
     2281 tagagaggtt gttgcagcgt tagagcaagc aggggtgtca ttcattacag tgcatggcag
     2341 aactcgcgac cagttttaca gcggaaaagc agattgggat gcgataggta caatagctga
     2401 ttttagcaaa gtccctattg ttggtaacgg cgacatcgca agctatgaag atgccatgga
     2461 aaaactttct acaagcggtt gtgctgctgt gatgataggg cgtgccgcta taggcaaccc
     2521 atggatattt tgcgggcaac aaccatcata tcaggaaaag atagcgatga taaaaaaaca
     2581 ttatgaatgt atggtacagc attacggaaa ttatggcatt atactcatgc gaaaacatat
     2641 agtacactat ttaaaaggat tttccggtgc agttaaactg cggcagaaac tgctaacact
     2701 tacctcaatg gatgagatac tggaggtact acattattaa aatcaatctt tcatgggata
     2761 attctgctgc ataagcaata gctgcttgta tatcttcaga tgtcaatgaa ggataactcc
     2821 tcactatatc ttcttctttc atcccattag ctaaattatc caatataacc gaaactataa
     2881 ttcttgtgcc tttaatacat ggttttccat gacatatgtt tggatcaatt attattctgt
     2941 ctttccaatt catacttacc tcctgctaca tataagctta ctatacaaca attataaaaa
     3001 caattatttt tcatctttaa caacttccgc atcaataaaa tatcagatat tataaccttt
     3061 cacgcaataa tcctatcatt tcatcatcat tcaggttaac aggattattt ttgttatcag
     3121 cttctttgat tattgtagca aaatcctgtt cccttactcc atacattgaa aggcgtggca
     3181 tggctaatgt ttccgt
//
LOCUS       DAOSOX010000245         3182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_30382_length_3182_cov_4.970259, whole genome shotgun sequence.
ACCESSION   DAOSOX010000245 DAOSOX010000000
VERSION     DAOSOX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30382_length_3182_cov_4.970259"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLX22_11815"
     CDS             complement(<1..229)
                     /locus_tag="PLX22_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20625.1"
                     /translation="MPDDSQNIIESKHLILPSEILPEIIPIIPISHRPIFPGMMVPLV
                     LTGDTMLNTARAIAESDNKIGGVVLIQNQREG"
     gene            485..1465
                     /locus_tag="PLX22_11820"
     CDS             485..1465
                     /locus_tag="PLX22_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOT20626.1"
                     /translation="MLKRYYGALFITFVISTLYACKQQQNAVTARLIFMQGDVTIIQN
                     DINKLAGIGALVSPDDMITTGDKSIAVVQIADRAIAHIGSNSKVIVKSLLDTSVNLYL
                     EKGEVISKVERLQKGHEYMIKTRSVVASVRGTQFLVKSDEKIGKVAVNTGSVMVQPVI
                     ETEKGVVKESVIEGGKQAIVTVEEEKVTEEVPVTIQKISVKDKMKMEAMINLDVIPQD
                     VIMQPEAFEKVQETIKQNVEKMQEIESLSEDTIKQQIKKERIERLMQQKTRTLEEIKE
                     ACERIDVVKLYSGKEIQGAIISRGESYKILTTTGVVDVAKKDVRSISVIR"
     gene            1676..2770
                     /locus_tag="PLX22_11825"
     CDS             1676..2770
                     /locus_tag="PLX22_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20627.1"
                     /translation="MNIIQQMYASLCLVVVCSIVVLVAPVYGESIFLKDGSIVEGDIV
                     KETDKAMDVKLPDGNKITIPRKDIVRTLVNTNYKTKMYIMKTNKEVLPVYIVEEDNES
                     YTYRNELQSADEYKISKGDVLFLSKIPPQDWIADEVQKKVNELGVKKHYTWEQKVKWR
                     APLFRFGYSNIGKYADSDIEDAYSDSRNINAFIDLFPLRFRNESGSGFDVMMRVRAVG
                     NFDEPELYDEKTQLLSKLFDVTIGEEYEAQFNIGMIGGGIRYAYTLYYGVAIQPYVFG
                     LINYVVIDQIKVESTTETESFDEKISSSKYGFQAGVGVDIGLTPHIGIFVEGMYGYIS
                     AKFNDGKKHNIDGYYIYYGVTWRTSYGLID"
BASE COUNT         1116 a          424 c          659 g          983 t
ORIGIN      
        1 tcccttcacg ctggttttgt attaaaacca caccgcctat tttgttgtcg gattctgcta
       61 ttgccctggc agtattgagc atagtatcac ccgttagtac aagcggcacc atcatgcccg
      121 gaaatattgg cctgtgcgat atcgggataa taggtataat ttcaggtaaa atctcagatg
      181 gcagtataag atgttttgat tcaataatat tctgagaatc gtctggcatg agctatcacc
      241 tcttgtgaat tgtttttata gtagtgtagc atggcagatt tttaaaatca agataatttt
      301 ttttggcagg catatggtaa gtggttacac actggttatt tttagtccag acgtctttaa
      361 atatttttaa aaaaaagatt tacagattaa caattatatg ttgataatac tatataataa
      421 atgctggtat tacattatta cactgctaat taaatatcaa aaaattatca atcgaggtgt
      481 agctatgctt aaacgatatt atggtgcgct ttttattaca tttgttatat ccactttgta
      541 tgcctgtaag caacaacaaa atgctgttac agcacgtctc atttttatgc aaggggatgt
      601 aacaattatt cagaatgata taaacaaact agcaggtata ggagctttgg ttagtccaga
      661 tgatatgatt acaactggtg ataaatccat agcagtggtt caaattgctg atagggcaat
      721 agcccatatt ggcagcaaca gtaaggttat tgtaaaatca ttactggata catcggtaaa
      781 cctgtatctg gaaaaaggtg aggttatatc caaggttgaa cgcctgcaaa aagggcatga
      841 gtatatgata aaaacgcgtt cagtggttgc tagtgtccgc ggtacacagt ttttagtaaa
      901 atccgatgaa aagattggca aagttgcagt taataccggc agtgtaatgg tacaacctgt
      961 tatagaaact gaaaagggag tagtcaaaga atcggtaata gaaggaggca agcaggcgat
     1021 agtaactgtt gaggaagaaa aggttactga agaagttccg gttaccatac aaaagatatc
     1081 ggtaaaggat aaaatgaaaa tggaggctat gattaacctt gatgttatac cacaggatgt
     1141 tattatgcaa cctgaagcat ttgagaaggt gcaggaaaca ataaaacaaa atgttgaaaa
     1201 aatgcaagag attgaatcgt tatccgaaga cacaataaaa cagcagataa aaaaagagcg
     1261 tatagaacga ttgatgcaac agaaaacaag aactttagag gaaataaaag aggcatgtga
     1321 gcgaatagat gtagtaaaat tatattcagg taaagaaatt cagggggcaa ttataagcag
     1381 aggtgaaagt tacaagattt taacaacaac aggggttgtt gatgttgcca agaaagatgt
     1441 acgatcaata agtgttatca ggtaaattat tgtaatagac atgttaaaca acatatccat
     1501 tacacaatgg agatgccggt ttattagtat cgtatatttt tgtttttggg tgggattgcc
     1561 aatgactgta ttgcagccac ccataagaaa tgaacgttgt acaaacctca aacaatgccc
     1621 tcctcgcttt acgaagggga ggggtgaagt taataaattt attttaggag gagttatgaa
     1681 tataatacaa caaatgtatg catcattatg tttggtggtt gtatgcagca tagtagtact
     1741 tgtagcaccg gtgtatgggg aatctatttt tttgaaagat ggtagcatcg ttgaaggtga
     1801 tattgtcaag gaaacagata aagcaatgga tgtaaagttg cccgatggta acaaaataac
     1861 aattcctcgc aaagatattg tgcgaacatt ggttaacaca aattataaga caaagatgta
     1921 cataatgaag accaacaaag aggttttgcc ggtatacatt gttgaagagg ataatgaaag
     1981 ctacacctac cggaatgagt tgcaatcagc ggatgaatat aaaataagta aaggagatgt
     2041 attatttctt tcaaagatac caccacaaga ttggatagca gatgaggtac aaaagaaggt
     2101 aaatgaatta ggtgtaaaaa agcattacac ctgggaacaa aaggtaaaat ggcgtgctcc
     2161 attatttcgt tttgggtatt ctaatatagg aaaatatgct gatagtgata tcgaagatgc
     2221 gtattcagat agccgtaata tcaatgcttt tatagatcta ttccccttac ggttcaggaa
     2281 tgaaagtggt agcggatttg atgtgatgat gagagtacga gctgtaggta attttgatga
     2341 acctgaacta tatgacgaaa aaacgcaact attatctaaa ctatttgatg ttactatagg
     2401 tgaagagtat gaagcacaat tcaatatagg gatgataggt ggaggtatac gatatgcata
     2461 cacactatac tatggcgtgg cgatacaacc gtatgtattt ggactaataa attatgtggt
     2521 gatagatcaa attaaagtag aatccacaac ggaaacagaa agttttgatg aaaaaatatc
     2581 ttcctcaaaa tatggatttc aggctggggt aggcgttgat ataggattaa ctccacatat
     2641 tggtatattt gttgaaggaa tgtacgggta tatctctgca aaatttaatg atggtaaaaa
     2701 acataatatt gatggttact atatttacta tggtgtaaca tggcgaacat cgtatgggct
     2761 tatagattaa ttgtaaactt atcaatgctt atgcttggat aatacatttt ttgtttatat
     2821 cattgctatg gctccaataa tgttaattaa atattattgg agccatttta ttattataaa
     2881 tgtaactcat tcctatgtat agatagttta ttatcaattc taaccattca taatatgaaa
     2941 ataaaatagt aaaatatcaa tggtatagct gtaaaagtac acactttcat ggtaatagta
     3001 ttaaatcatt taatcatgat ttcaacgctt ttaacctgta taaatagaca tctgattact
     3061 ttggttttct atgaattttt tattgacagg tataaatggg ctgctgtgag tatacagtta
     3121 ttaagatttt ttttatgcat tacatagatt tttagtttta tactttttat atgttcgata
     3181 gt
//
LOCUS       DAOSOX010000246         3170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_30548_length_3170_cov_7.926806, whole genome shotgun sequence.
ACCESSION   DAOSOX010000246 DAOSOX010000000
VERSION     DAOSOX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30548_length_3170_cov_7.926806"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLX22_11830"
     CDS             <1..615
                     /locus_tag="PLX22_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT20628.1"
                     /translation="PGNTVNVYGPGTFEKSLEDIIKGQMQYSYFPVRTEELRATLHFY
                     EIKNNQFTVGNFNVYTHLLNHPVTCYGYKVVCNGKIFVYLGDNEPYYNVYKDNDPEVI
                     AFAKQMNEQLVDFVKGAHVLVSDAQYIPAEYPKKTGWGHSTTHHVINLALKAKVKTLF
                     LFHHEPLRKDSELDAIVTHYRNILQKKGLPLELYAAAERNSYTF"
     gene            complement(616..1542)
                     /locus_tag="PLX22_11835"
     CDS             complement(616..1542)
                     /locus_tag="PLX22_11835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT20629.1"
                     /translation="MLNKIMIPFFKILFYNAIVTGSVCFVLHLHHIVLPIQCILYILA
                     ITIISSIYITYTKAIPLQLLLHTIKTIVDTKHKTYSISLKETAIKLSHLLEQFKMKSD
                     AVMKEKDTIVHELTESKKLLEKYSTNLEEMVEQRTEILKWLSITDPLTGLYNRRYFIE
                     QIELEFKRSKRYSRDLSLLMLDIDHFKLVNDNYGHQIGDIVLRKISSVIINLLRDSDL
                     AFRYGGEEFMIILPETKSEDAINVAERMKQEIMNTQHNYSSINFTVTASFGIVSIKDM
                     IDKFETIDDIIKKVDDNLYKAKNSGRNTIIYE"
     gene            complement(1561..2340)
                     /locus_tag="PLX22_11840"
     CDS             complement(1561..2340)
                     /locus_tag="PLX22_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOT20630.1"
                     /translation="MEQPFYIVYDRLYTTNYITASVEKSERVRQIMSSLGKTYPIVKP
                     DPCTDEDILRCHSEGLLFMEKQDPERYEIAKLSAGGALKAATMAMENKPCFAVIRPPG
                     HHANPDHNWGFCYFNNMGIALNHLIAKGIITRAIVLDIDLHFGDGTDTIFKSNANVPV
                     LNIQASNPVDFINETKSRLESCDHADILGISAGFDQYIKDWGANLSTPDYFTLGEISG
                     SFAKKRCNGHIFAILEGGYYIPDLGKNVIALIEGILKGLYD"
     gene            complement(2419..3006)
                     /locus_tag="PLX22_11845"
     CDS             complement(2419..3006)
                     /locus_tag="PLX22_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20631.1"
                     /translation="MEITDTINYYVEPVEIEIYLKKAGKVRTIIKDMFVELIDVEPLP
                     NETSKKIFEHFKSNNQPIDLIEITNLFPELISMVFESYYHNINLYEKLSLYFKAGLSG
                     STDSWRLALYFTELLMKFEPTIASSQYIGDFQTYNLNYLIRKLNQLGEKFLLEDATVL
                     YLIKRRNKAYEGKPRDKEFEKLVELWQFNVKEHPF"
BASE COUNT          976 a          537 c          557 g         1100 t
ORIGIN      
        1 cctggcaata ctgtcaatgt atatggtccc ggaacatttg aaaaaagttt agaagatatc
       61 ataaaaggac agatgcaata cagttatttc cctgtacgta ctgaagagtt gcgggcaaca
      121 ttacattttt atgaaataaa aaataatcaa tttactgttg gaaattttaa cgtttacaca
      181 catcttttaa atcatcctgt tacatgttat ggatataaag tagtatgcaa tggtaaaata
      241 tttgtttatc tgggtgataa tgaaccatat tacaatgttt ataaagataa tgatcccgag
      301 gttattgcat ttgcaaaaca gatgaatgaa caacttgttg attttgttaa aggtgctcat
      361 gttttggttt ctgatgcaca gtatatccct gctgaatatc caaaaaaaac agggtggggc
      421 cacagcacta cccatcatgt cattaattta gctttaaaag caaaggtaaa aacattgttt
      481 ttatttcacc atgaaccatt gaggaaggat tcagaattag atgcgatagt tactcattat
      541 agaaatatat tacaaaagaa agggctcccc ttagagttat atgctgctgc ggaaaggaat
      601 tcatatacat tttaattact cgtatattat agtgtttctg cctgaatttt tagccttata
      661 gagattatca tccacttttt taataatatc atcaattgtt tcaaatttat caatcatatc
      721 tttgatggaa acaatcccaa agcttgctgt aacagtaaaa ttgattgagc tatagttgtg
      781 ctgtgtattc attatttcct gtttcatcct ttcggctaca ttaatggcat cctcagattt
      841 tgtttccggt aatattatca taaattcttc tccaccgtat cgaaaggcta aatcagaatc
      901 acggagcaga ttaataataa ctgaagatat ttttctaagc acaatatcac caatctgatg
      961 gccgtaatta tcattaacaa gcttaaaatg atcaatatct aacatcagga gagataagtc
     1021 tctgctataa cgttttgacc gtttaaattc cagttcaatt tgctcaataa aatatcgacg
     1081 gttgtaaagc cctgtcaatg gatcggttat ggatagccat tttaatattt cggtccgctg
     1141 ttctaccatt tcttccagat ttgtagaata tttttccaac agtttttttg attcggtcag
     1201 ctcatgaaca attgtatctt tttctttcat aacagcgtct gatttcattt taaattgttc
     1261 cagcaaatgg gatagcttta ttgctgtttc ttttaacgat atactatagg ttttatgttt
     1321 agtatctact atcgttttga tagtatgcag caacaattgc aaaggtatgg ctttagtata
     1381 ggttatgtat atgctgctta taatggtaat ggcaaggatg taaaggatac actgtattgg
     1441 cagtacaatg tgatgtaaat gcagaacaaa acataccgaa cctgtcacta ttgcattata
     1501 gaatagtatc ttgaagaaag gaatcataat cttattaagc ataaaattgt tagcatggta
     1561 ttaatcgtat aaacctttta gtattccttc tataagggca ataacatttt tgcctaaatc
     1621 agggatatag tatccgcctt ccaatatggc aaatatgtgc ccattgcatc gctttttggc
     1681 aaaagaacca gaaatctccc ctaatgtaaa gtaatccggg gttgaaagat tggcgcccca
     1741 atcttttata tattgatcaa aacctgcact tatccctaaa atgtctgcat ggtcacagct
     1801 ttccaatcgg gattttgttt cattgatgaa atcaaccgga ttggatgctt gaatattcag
     1861 aacaggaaca ttggcatttg atttaaatat ggtatcagtt ccatcgccaa aatgcaaatc
     1921 aatatcaaga actatcgccc ttgttatgat tccctttgcg atgagatggt ttaatgcaat
     1981 acccatattg ttaaaataac aaaaacccca gttatgatca ggatttgcat gatgtccggg
     2041 gggacgtatt actgcaaagc agggcttatt ttccatagcc atggttgctg ctttcaaagc
     2101 accaccagcc gaaagttttg ctatttcata gcgctcgggg tcctgttttt ccataaatag
     2161 caacccctcg gaatggcatc gtaaaatatc ttcgtcggtg cagggatcag gtttaacgat
     2221 agggtaggtt ttccccaatg agctcattat ctgacgaaca cgctcagact tttcaactga
     2281 tgctgttata tagttggtgg tgtaaagcct atcgtatact atataaaagg gttgctccat
     2341 aaataaatac ctgttgggat ttttaaggtg tacttttact aaaaataagc tatcagtatg
     2401 gaaatgtcaa attacttttt aaaatgggtg ctctttaaca ttaaattgcc atagttccac
     2461 cagcttttca aattctttgt ctctgggttt accttcatag gctttattcc ggcgtttgat
     2521 gagatacaaa actgttgcat cctccagtaa aaatttttca ccaagctggt taagcttacg
     2581 gataagataa ttgaggttgt atgtttggaa gtcaccaata tactgggatg aagcgatagt
     2641 aggctcaaat ttcatcaata gctcggtaaa gtaaagtgct aaacgccatg agtcagtgct
     2701 accactcaaa cctgctttga agtacaggct caatttttcg tagagattga tattgtgata
     2761 atacgattca aataccatgg atattaattc agggaaaagg tttgttatct caattaaatc
     2821 tataggttga ttattgcttt taaaatgttc aaatattttt ttgctggttt cattgggaag
     2881 gggttcaacg tcaatgagct caacaaacat atctttgatg atagttcgca ccttacctgc
     2941 tttttttaaa taaatttcta tctcaacggg ctcaacataa tagttaatag tatctgttat
     3001 ttccattgtt attaaaggtt ttaaaaatta ttttttatta tttgctgtca tagtattcat
     3061 acgctgcatt aaggttttaa ggtattcttt ttgcttttga ctggtagctg tatcaaaagc
     3121 ttttttgagc aaagcctcgt cttttatccc ttgatcaatc tttttttcca
//
LOCUS       DAOSOX010000247         3112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_31345_length_3112_cov_7.810272, whole genome shotgun sequence.
ACCESSION   DAOSOX010000247 DAOSOX010000000
VERSION     DAOSOX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31345_length_3112_cov_7.810272"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLX22_11850"
     CDS             <1..259
                     /locus_tag="PLX22_11850"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT20632.1"
                     /translation="VAYLYGCGAQAVYIFGSLVYNTMFDEMSDVDIYVEGLPRGKQKG
                     LFSALDEIFGEIKFDLFFDDDVLKPEIAIKIKKEGKLWKPS"
     gene            244..720
                     /locus_tag="PLX22_11855"
     CDS             244..720
                     /locus_tag="PLX22_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20633.1"
                     /translation="MEALLADIKSDLTYLQKEKSSIQRKLLRLQSEKDPENIDSHIKA
                     IAGSLHAIYTGCENILERIVRTVDGDIPLGKDYHSLLLRRAITTIDAIRPEILSIETY
                     AKLDEMRAYRHKFRNIYLYLIAPARIIDLAQEGIALVDQFHNDIDVFCAFLSSKQP"
     gene            772..2316
                     /locus_tag="PLX22_11860"
     CDS             772..2316
                     /locus_tag="PLX22_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT20634.1"
                     /translation="MHKHTEEVTMLSRATSLVIHGIEAHLVDVEVDIVKGLPNFTIVG
                     LPDSIIRESKDRIRSAIENSGFEFPPKNFIVNLAPAGYRKEGANLDLPIAVSILVATG
                     QVESTQLTIPMVGELSLDGRVRPIKGVIAMAIALYHAGIKEIIVPYENRNEATAIEGI
                     KVYPVKDIAQAIEACNGSMQPYTEHVSGDSSWNGYLDMASVMGQETAKRAMEIAAAGR
                     HNILMYGPPGSGKTMLARCVPGILPPLTKEQAIMTTMIHSVGGVLAHGQGLVYMPPFR
                     APHHTSSDAALVGGGKIPNVGEVSLAHNGVLFLDEFVEFRNDVIQALRQPLEDCQITI
                     SRALGTMTFPADFMLVASSNPCQCGYLFDPEIPCKCTAGKIRNYFSKIAGPILDRIDI
                     EVLVGRVPYKDLLGKSNAESSARVRERVVKAREKQRQRFSGRTTTCNARMTSDEVKEF
                     CAINGECESILELAIKRMNLSARSFFRILKVARTITDLDDAESIEKRHILEALSYKNL
                     QRYYEI"
     gene            2433..>3112
                     /locus_tag="PLX22_11865"
     CDS             2433..>3112
                     /locus_tag="PLX22_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT20635.1"
                     /translation="MLCALFLAIGDALVKNAPAMHPATIAAGRVFFSLPVLWAIALVN
                     GIPAINGDIIYALLLSPPIEVVALVLYMRALQISPMYRTLPFLAFTPLLLIITSWVLL
                     HERASLSGIIGIVCVVLGTYSLYFQRGQSLLVPLKTVFHDKGSLMMLIVAFLYSITAN
                     FGKMGVMYSSPAFFGAVYFTIMVIILGIWCACVDSIKNIFTKNNIVIGLTQGIMITFH
                     MLAVAIAPV"
BASE COUNT          870 a          584 c          716 g          942 t
ORIGIN      
        1 agttgcctat ctatatggct gtggtgcaca ggctgtttat atatttggtt cattagtata
       61 taacaccatg tttgatgaaa tgtccgatgt tgatatatat gtggaaggat tgccacgggg
      121 aaagcaaaaa ggcttgttca gcgcactgga tgaaatattt ggtgaaataa aatttgatct
      181 gttttttgat gatgatgtgc tcaaacctga aattgctata aaaataaaaa aagaaggtaa
      241 gttatggaag ccctcttagc tgatattaaa tcggatttga catatttaca aaaagaaaaa
      301 tccagtatac agcgaaagtt gctccggttg caatctgaaa aggatcctga aaatattgac
      361 tcgcacataa aggctattgc cgggtcatta catgcaatat atactggatg tgaaaatatt
      421 ctggagagga ttgtccgcac tgttgatggc gatatcccat tggggaaaga ttatcatagt
      481 ttgcttttac gaagagctat cactacaata gatgccatta gacctgaaat tcttagtatt
      541 gaaacgtatg ccaaacttga tgaaatgcga gcataccgac ataaattcag gaatatatat
      601 ctttatttaa tagctccagc ccgtataata gatttagcac aggaaggtat tgcattggta
      661 gatcagttcc ataatgatat tgatgttttt tgtgcatttt tatccagcaa acaaccgtaa
      721 aaaataaaaa aattaaaaaa atttttaaaa ttttcccgca aaagcgtatt aatacataaa
      781 cacactgagg aggttactat gctttcgcgg gctacttcgc ttgttatcca tggcattgaa
      841 gcgcatctcg ttgatgttga agtggatatt gtaaaagggc ttcctaactt taccattgtt
      901 gggctgcctg attccattat ccgtgaatca aaggacagaa tccgttcggc aattgaaaat
      961 tcaggatttg aatttccgcc caaaaatttt attgttaatt tagcacctgc cggctatcgc
     1021 aaggaaggag caaatcttga tttgcccatt gcggtttcca ttctggtggc aacagggcag
     1081 gttgagagta cacagcttac catacccatg gttggtgagc tttcgcttga tggcagggtg
     1141 cggcccatta aaggtgttat tgcaatggca attgctttgt accatgccgg tattaaagag
     1201 attattgttc cgtatgaaaa ccgcaatgag gcaacggcta tagaagggat caaggtgtat
     1261 cctgtaaagg atattgctca ggcaatagaa gcctgcaatg gtagcatgca gccatataca
     1321 gaacatgtaa gtggcgatag ctcatggaat ggctaccttg atatggcaag cgtcatgggt
     1381 caggaaacag ccaaacgtgc catggagata gctgctgccg gacggcacaa tattttgatg
     1441 tatggaccgc caggatcagg gaaaaccatg cttgcgcggt gcgttccagg catcttaccg
     1501 ccgcttacca aagagcaggc aattatgacc accatgatac attcggttgg tggtgtgctt
     1561 gctcatgggc agggattggt ttatatgcca ccgttcagag ccccgcacca taccagctct
     1621 gatgcagcgc tggttggcgg aggcaaaata cccaatgttg gcgaggtatc attagctcat
     1681 aatggcgtac tgtttttaga tgagtttgtt gaatttcgta acgatgtgat acaggcattg
     1741 cgtcagccac ttgaggattg ccagattacc atatcgcgtg cgctgggaac catgacattc
     1801 cctgccgatt ttatgctggt tgcttccagc aatccgtgcc agtgcggcta cctctttgac
     1861 cctgaaatac cgtgcaagtg tacagcaggt aaaatacgca attacttttc aaagattgcc
     1921 ggccccatac ttgaccgcat tgatatcgag gtgcttgttg gcagggttcc ttataaggat
     1981 ttactgggca aaagcaatgc tgaaagctcg gcaagggtaa gggaacgggt ggtaaaagca
     2041 cgtgaaaaac aacggcagcg tttttccgga agaacaacca cctgcaatgc acgtatgaca
     2101 agcgatgagg tcaaagaatt ttgtgccatc aatggcgagt gtgaatcaat tcttgaactg
     2161 gcaataaagc gaatgaatct atctgctcgc agttttttta gaatccttaa ggttgcacga
     2221 accataaccg atcttgatga tgcggaatcc attgaaaaac ggcatatact ggaagcattg
     2281 tcatataaaa atctacagcg gtactatgaa atttaataat ggaatatatg tgttttacac
     2341 tatatttttc tattgacgaa agtccatgaa cataaaaaac ctttgtccat agtgttatta
     2401 aagggaggct ttctatctgg attgtttatg ctttgttatg tgcattgttc cttgctattg
     2461 gtgatgcgct ggttaaaaac gcaccagcaa tgcatcctgc aaccattgct gccgggcgtg
     2521 tcttcttttc attaccggtg ttatgggcga tagctcttgt aaatggtatc cctgctatca
     2581 atggtgatat catctatgca ttgttgcttt caccgcctat tgaggtggtt gcacttgttt
     2641 tatatatgcg tgccttacag atatcaccca tgtacaggac actcccgttt ctggcgttta
     2701 ccccgcttct tcttattata acttcatggg tgctattaca tgaaagggcg tccctatctg
     2761 ggattattgg tatagtatgt gttgttctgg gaacgtatag cctttatttt cagcgtggtc
     2821 aaagtttgct ggtgccattg aaaacggttt tccatgataa gggttcgtta atgatgctca
     2881 tcgtggcgtt tttgtatagt atcaccgcaa attttggcaa gatgggtgtt atgtactcaa
     2941 gccccgcttt ttttggcgct gtgtatttta ccataatggt tattattctt ggtatatggt
     3001 gtgcctgtgt tgattctatc aaaaatattt ttacaaaaaa caatattgtt attggattaa
     3061 cgcaggggat tatgataaca ttccacatgc ttgctgtggc gatagctccg gt
//
LOCUS       DAOSOX010000248         3084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_31699_length_3084_cov_11.466491, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000248 DAOSOX010000000
VERSION     DAOSOX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31699_length_3084_cov_11.466491"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLX22_11870"
     CDS             complement(<1..251)
                     /locus_tag="PLX22_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20636.1"
                     /translation="MDKKHDKRVGRFLVENNIVTEKQLQEALELQHDNPERLIGEILV
                     TLGVLTKEELIMALEMYMMTTDAVPDHVDEWLDQDEIDL"
     gene            406..984
                     /locus_tag="PLX22_11875"
     CDS             406..984
                     /locus_tag="PLX22_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HOT20637.1"
                     /translation="MKIRIFITALILCLAFIGAQKNVSFVGVSVCKKCHESEAIGNQY
                     KFWLQSPHAKAYLLLKQDKALELAKELCIQNPHSNELCLKCHTTGFGKNPALWDDGVG
                     CESCHGPGSIYSSFENHVSFDNRETAYKKAVSKGMYPIRGTDGIKAREKVCRSCHNEK
                     RPCYPKDPALQQHQFLPLQLIADFVFPHRLRR"
     gene            complement(999..1922)
                     /locus_tag="PLX22_11880"
     CDS             complement(999..1922)
                     /locus_tag="PLX22_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20638.1"
                     /translation="MQKFIFISNDEHKRNELTKQLDCVDCIVWDEQTTTINKWVNDDM
                     CTIIDVELLPAIHGIGSVIDEFSRRKKEIIGVSSKSSISEIDKKNIVQYGITAVFPLS
                     QCRYIPPYCDKYQGLVSGIIGIVDNNIFHCQGLSTLIKKFGYDAIVMNDLKDCCSADN
                     SMDILCINCSQVSPHEIAAMYIGGKLPKKNAMVLYKSHEDDIFIHDILKLNRIVKVIY
                     TLEEVYAVLLQLLFRQQFHALVYSLYESSDMQSSATSYRGNLRQWYWEAGMDIFTMAD
                     VLQPELVSTYQKHIQLMHALMTRAMAFQWMR"
     gene            complement(2348..2608)
                     /locus_tag="PLX22_11885"
     CDS             complement(2348..2608)
                     /locus_tag="PLX22_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20639.1"
                     /translation="MQKLSLIWGILALLSMLTIFIPYLNSFIMIAILFSVAGLIISIV
                     ALTIAKDNKGFAIAGIICCASAVIFGLLIVAYFVFDVEVKSL"
     gene            2762..3019
                     /locus_tag="PLX22_11890"
     CDS             2762..3019
                     /locus_tag="PLX22_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20640.1"
                     /translation="MEDDCNEVVKALAAKETVAKIIIGILAVLYVFLLYGSFNAYRAP
                     GKVSQEMLEQTEKLSSEQQKALEQLQQLQKLLEKMQQEEKE"
BASE COUNT         1058 a          591 c          480 g          955 t
ORIGIN      
        1 aataaatcaa tctcatcttg atccagccat tcatcaacat gatctggtac ggcatcggtg
       61 gtcatcatat acatttccag cgccatgata agctcctctt tggtaagcac acccagagtt
      121 acaagaattt caccaataag cctttcagga ttgtcatgtt gcaattctaa tgcttcctgt
      181 aactgttttt cagttactat attgttttct accagaaaac gtccaactct tttatcgtgt
      241 tttttatcca taatagtaac attactgtat tagcttattt actattggat agtaatagca
      301 aaaattaaat aagtcaagtt aaaaaaattg acaataacta tcgcccacaa taaaaattat
      361 ttacattttt ttattttata gttagatagt tactatggta ttattatgaa aatacgaatt
      421 tttataacag ccttgatact ttgtcttgcc ttcattggtg cacaaaaaaa tgtatctttt
      481 gttggtgtat ccgtatgtaa aaaatgtcac gagtctgaag ctattggcaa tcaatacaaa
      541 ttctggctac agtctcctca tgcaaaagca tatctcttac ttaagcagga caaggcactt
      601 gaactggcaa aggagctatg tatacaaaat cctcacagta atgaattatg cctcaagtgc
      661 cataccactg gatttggcaa aaatcctgct ctatgggatg atggcgtagg atgcgagtcc
      721 tgccatgggc caggaagcat ctatagtagt tttgaaaatc atgtttcgtt tgataatcgc
      781 gagacagcct acaaaaaagc agtgtcaaaa ggcatgtacc caatacgggg aactgacggg
      841 ataaaagcac gcgaaaaggt ttgccgcagc tgccataatg aaaaaagacc atgctatcca
      901 aaggaccctg ctttgcaaca gcatcaattt ttgccgttgc aactaattgc tgattttgta
      961 tttccccatc gtttgcgccg gtaacactat tatcaggact atcgcatcca ttggaaagcc
     1021 atagctcttg tcatcaaagc gtgcattaat tgaatatgct tttgatacgt gctaacaagt
     1081 tcaggttgta gcacatcagc catggtaaaa atatccattc cggcttccca ataccattga
     1141 cgcaagtttc ctcgatatga tgttgcgcta ctttgcatat ctgatgattc atataaagaa
     1201 tataccaatg catggaattg ttgcctgaat agaagctgta ataatactgc ataaacttct
     1261 tccagtgtat agataacttt tactattcgg ttaagtttca gtatatcatg aatgaatata
     1321 tcatcctcat gacttttata caataccatt gcattttttt ttgggagctt tccccctatg
     1381 tacatagctg caatttcatg agggcttacc tgtgaacaat taatgcacaa aatatccatt
     1441 gaattgtctg cactgcaaca atccttcaaa tcattcataa caattgcatc atagccaaac
     1501 ttttttatta atgtcgatag tccctgacaa tgaaatatat tgttatccac tatgcctata
     1561 attccggaaa caagtccctg gtacttgtca cagtagggag ggatatacct gcattgagat
     1621 aatgggaaaa cagcagtaat accatactgg acaatatttt ttttatcaat ctcacttatt
     1681 gaagattttg agctaacacc aataatctct tttttgcgtc tggaaaactc atcaataact
     1741 gacccaattc catggatggc tggtaataac tcaacatcaa taatagtgca catatcgtca
     1801 ttaacccatt tattaatcgt ggttgtttgc tcatcccata ctatacaatc aacacaatct
     1861 aattgttttg ttagttcatt tcttttgtgc tcatcattgc tgataaatat aaacttttgc
     1921 atttttttca ccgatttaca attgctggaa aaaaccaaaa tataatttac gcttttttgt
     1981 ataataaaaa ggtaccaccc ccgaagatgg gaaccatcaa aaggggagga ccaccatgta
     2041 caaagagtat aaaataattt atgtaaacat gtttaaggta aagctataca atacaaagaa
     2101 aatgctcctg tcatcccaca caaaacacac tttccattat cgttatgcac tacagacatt
     2161 tttgaagaca aaaataagat ttttttctct tctattctca caattatagt tacaatcgtt
     2221 aacgatagta tgctaacaat tatccataca ataaatactg gtaaaacaat gtcatgcata
     2281 taatttttat taactgtgat agtaattttg ctaacttctc ttttcaatca tttattccat
     2341 tcataaatca taaacttttt acttctacat caaaaacaaa atatgctaca attaataatc
     2401 caaaaatgac ggcacttgca caacatataa tgccagctat cgcaaatcct ttattgtctt
     2461 tagctatagt taatgctaca attgaaatta ttaatccagc aacactaaat aaaatagcta
     2521 tcataataaa agaattaaga tagggaataa agatcgtaag cattgaaagt aaagccaaaa
     2581 ttccccatat taatgaaagt ttttgcattg tatcctcacg aacccgtcct caaaccaatt
     2641 gggaatgagt agcatcgccc cggatattgg ggtataatgt cccaaagtaa caataaacat
     2701 aaatactacc gtaaagtgta aaacagaagc cacaaaaaag caaccagaaa aaattgtatc
     2761 agtggaggat gattgtaacg aagttgtcaa agcgcttgcc gcaaaagaaa ctgtagcaaa
     2821 aataatcata gggatattgg ctgtcctgta tgttttttta ctatacggtt ctttcaatgc
     2881 ataccgagcc cccgggaaag ttagtcagga gatgttagaa caaacagaaa agctttcatc
     2941 agaacaacaa aaagcattgg aacagctaca gcaattacaa aaattactgg aaaaaatgca
     3001 gcaagaagaa aaagaataat tcatataacg gttacatcct ctgtgcatag aggatgtaac
     3061 catttaaatc ttctttacct tata
//
LOCUS       DAOSOX010000249         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_32026_length_3059_cov_7.260652, whole genome shotgun sequence.
ACCESSION   DAOSOX010000249 DAOSOX010000000
VERSION     DAOSOX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32026_length_3059_cov_7.260652"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1236
                     /locus_tag="PLX22_11895"
     CDS             361..1236
                     /locus_tag="PLX22_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HOT20641.1"
                     /translation="MEKEDSKKKISFRQKNATICPVCGYEFYREEMLTGGGRLIAGKL
                     TDELRRTYEKNEKWGVIYPLAYVVTVCPRCLYAAYPKDFSNLQSEDLQKIQATANARK
                     QSIEKFFGKLDFNIDRGLYHGAASYLLAMDCYSFRNKMVAPTFKMAISAIRAAWLFGD
                     LAKLEPDTPYKKISDFFYKKAYDLYFKVVDIMQTGAEPVDAAGNIGPDIDKNWGYDGI
                     LYMCAILTLKVGAKEPDLKKRIENFERTKRYLSKLFGSGKASKNKPGPLLDMTRDLYD
                     KMNEMLEQWKNETPV"
     gene            1212..1994
                     /gene="truA"
                     /locus_tag="PLX22_11900"
     CDS             1212..1994
                     /gene="truA"
                     /locus_tag="PLX22_11900"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT20642.1"
                     /translation="MEERDSRLRYALIVQYDGTHYSGFQIQHNANTIQYELEKAIKIL
                     TREDIRIAAAGRTDAGVHALGQVIHFDLSKLIPLEKMCIGLNGILPADIAIKKAFNVD
                     SHFHARYSAVAREYSYVLYNHRYKSPFMLYRALWVREPINIAFFKEACQYCIGEKDFS
                     SFCKKSSATEGTIRFVEYITVDRIFNDFVVLTIKGNAFLHNMIRILVGTLLMLMNNNE
                     HPLKMKEILDRCDRNYSGETAPAYGLYFKKVYYPAVYDFLNV"
     gene            2164..2736
                     /locus_tag="PLX22_11905"
     CDS             2164..2736
                     /locus_tag="PLX22_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20643.1"
                     /translation="MKNNVLYGMLTILLVFIIAGNGYAQRKKGDYFLKPQIGMWFGPV
                     TPVFSTADDVDTDLWGGGFMRFNISSLPLKIGFDSSYEHFKSEGIKELTIIPVYGSLL
                     WRLPIDMPIVFQLKAGAGSAKVMIKPENMEQWDPMGMLGFEISFPAGKWINIGLRIDY
                     LFIYEEYRKGASKNGHIVNTGLTLYLNIGI"
     gene            2743..>3059
                     /locus_tag="PLX22_11910"
     CDS             2743..>3059
                     /locus_tag="PLX22_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20644.1"
                     /translation="MKKYLKFLYALAIVILIGCTGDNALFDELSNSRVTVIIKGTFES
                     TTDSSSIKGWDTTVSATQSIDDSVNDMITDISETFPNTFMVDIAEMRLAKEDGGSDDK
                     VAY"
BASE COUNT          997 a          448 c          657 g          957 t
ORIGIN      
        1 gataatgtta aaaaataatc gttttgtgct ttaacagccg ttgaaattgc aaatagtggc
       61 tgtgcaacgc acaccataat cagtacaata ataagcttga tagtatggtt cttcataaac
      121 attaccttcg gtgatattct ttttaaattt atcgacacgc gaaagttttc gcttgatata
      181 aaaatatcat aatgctttat ctaaaataga aagtctatta gatttttatg aaatttcaca
      241 ggaactatcg ccaaaaattt attttttgat agttattggg caattattta gttgcaaaga
      301 ttatctggta tgatagttaa tgataaaaat gatagtagta caaatatcag gatgtagggt
      361 atggaaaaag aggatagtaa aaagaagata tcattccgac agaaaaatgc tacaatttgt
      421 ccggtatgtg gatatgagtt ttatcgtgaa gaaatgctaa caggaggtgg gcggcttatt
      481 gcagggaagc taaccgatga attgcggcgc acctatgaaa agaatgaaaa atggggtgtt
      541 atttatccac tggcatatgt agtaactgtt tgtcctcgat gtttatatgc tgcctatcct
      601 aaagattttt ctaatttaca gtcagaagat ttgcagaaaa ttcaggcaac agcgaatgca
      661 cgaaaacaat caatagaaaa attttttggg aagctggatt ttaatatcga taggggttta
      721 tatcatggag ctgcttcata tcttcttgct atggattgct attcatttag aaataaaatg
      781 gttgctccta catttaaaat ggctatatca gcaatacgtg ctgcatggct ttttggggat
      841 ttagctaaac tggagcctga tacgccatat aaaaagattt cagatttctt ttataaaaaa
      901 gcgtatgacc tttactttaa agtggtggat attatgcaaa caggagccga gccggtagat
      961 gcagcgggga atattgggcc tgatattgat aagaactggg gatatgatgg tattttatat
     1021 atgtgtgcaa tacttacatt aaaagttggt gctaaagaac ctgatttgaa aaaaaggatt
     1081 gaaaactttg aaaggacaaa acgttacctg agcaaactct ttggtagtgg aaaagcatca
     1141 aagaataagc ccgggcccct tcttgatatg acccgcgatt tgtatgacaa gatgaatgag
     1201 atgctggaac aatggaagaa cgagactccc gtctgaggta tgcgttaatt gtccaatatg
     1261 atggtacaca ctatagcgga tttcaaatac aacataatgc taataccata caatatgaac
     1321 tggaaaaagc aatcaagata ctaaccaggg aggatataag gattgctgca gcaggcagaa
     1381 ccgatgctgg tgttcatgcg ttagggcagg ttatccattt tgatttgtcc aaactcatcc
     1441 ccttagaaaa gatgtgtatt gggcttaatg ggattttacc tgctgatata gctataaaaa
     1501 aagcatttaa tgttgatagc cattttcatg cgcgctatag tgctgttgct cgtgaatata
     1561 gctatgtact ctataatcat agatataaaa gtccgtttat gctctataga gcattatggg
     1621 tacgtgaacc tatcaatatt gcatttttta aagaagcctg tcagtattgc attggtgaaa
     1681 aagatttttc atcattttgt aaaaagagtt cagccacaga gggtactata cgatttgtgg
     1741 aatatattac agttgataga atctttaatg atttcgttgt tttaacaata aagggaaatg
     1801 cttttttaca caatatgatt agaatacttg tcgggacact ccttatgctg atgaataata
     1861 atgagcatcc tcttaagatg aaagagatat tagacagatg tgacaggaat tatagtggtg
     1921 aaacagctcc tgcatatgga ttgtatttta aaaaggtata ttatccggca gtatatgatt
     1981 ttttaaatgt gtaactaaac tgtatgttat gatacagatt atacaaaagg ttacaggcaa
     2041 taaagttaat accgataact atcgccgaaa gtattttaaa aatagtgatg agcattaaat
     2101 atgtattgac aggtatacca agtatgcaaa gtttagtggt aattaataaa ataggggttt
     2161 ctggtgaaaa ataatgtatt gtatggaatg ctaactatac tgcttgtttt tattatagca
     2221 ggcaatggat atgcacagcg gaaaaaggga gattattttt taaaaccgca gattggcatg
     2281 tggtttggtc cggttacacc agttttttca accgccgatg atgttgatac tgatttatgg
     2341 ggtggcggtt ttatgcgctt caacatatcg tcacttcctt taaagattgg atttgattct
     2401 tcgtatgaac attttaaatc ggaagggatt aaagagctga caattatccc tgtttatggc
     2461 agcctgctat ggcgtttacc tattgatatg cccattgtat tccagcttaa agcaggtgct
     2521 ggcagcgcaa aggtaatgat aaaaccagaa aacatggagc agtgggaccc tatgggaatg
     2581 cttggctttg aaatatcgtt ccctgctggc aagtggataa acattgggtt gcgtattgat
     2641 taccttttta tttatgaaga ataccgaaag ggtgcgagca aaaacgggca tattgttaat
     2701 accgggctca ccctttatct caatattggg atttagataa ttatgaaaaa atatctgaaa
     2761 tttttatatg ctctggcgat agttatcctt ataggttgca ccggtgataa tgcattattt
     2821 gatgagcttt ccaacagccg tgtgacggtg attataaaag ggacatttga atcgacaaca
     2881 gacagcagca gtataaaggg ttgggatact actgtatcag caactcaatc aattgatgat
     2941 tccgtcaatg atatgattac cgatatttct gaaacctttc ctaacacatt tatggttgat
     3001 attgccgaga tgcggcttgc aaaggaggat ggcggcagcg atgataaagt ggcatatga
//
LOCUS       DAOSOX010000250         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_32302_length_3041_cov_5.216343, whole genome shotgun sequence.
ACCESSION   DAOSOX010000250 DAOSOX010000000
VERSION     DAOSOX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32302_length_3041_cov_5.216343"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..2032
                     /locus_tag="PLX22_11915"
     CDS             317..2032
                     /locus_tag="PLX22_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT20645.1"
                     /translation="MSKKFIPDWTEKAPEPYSYRSIFKWGDPHSFKHPNPKLYQELKT
                     VFNLDDSYFAKKHSEGRNTVAYKKPVRLTKKQLQDIADIAGYENISTTQFDRLKFATG
                     KTIEEAMKLRKGIVERVADCVVHPRSVDDVEKLIQYCHRHKIPVYVYGGGSSVNFGLR
                     TAKGGITLVLSTHMNNILHVNEKNQTVTVQAGIQGPALEEALNNAKKHFGTKYNYTCG
                     HFPQSFEYSSIGGWIVTLGSGQSSSYYGDAYDLVISMKWVTPAGTFTTRDYAATATGP
                     KINDIMKGSEGTFGVLVEVTWKIYRYMPQNRRRFGFIFKTFQDAINASREISQGEFGM
                     PAVFRVSDEEETHIGLKLYGIDGTILDKLMQLRGFKPMQRCLFISSAEGEKHFTKNIK
                     RQVKKICKKYGGMYITGLPTLMWEPGRYKDPYIREDLHDYGILIDTLETSVKWDNLHD
                     VHKAVRQFIKNRPQTVCMTHASHFYPQGTNLYFIYIMKTDDMNEYITFQDGIIDSIAK
                     SGGSLSHHHGVGRMLAPWMEEHIGKEQMAVLRAIKKHFDPNAIMNPGGQLGLDLKGKD
                     WRSIK"
     gene            complement(2208..>3041)
                     /locus_tag="PLX22_11920"
     CDS             complement(2208..>3041)
                     /locus_tag="PLX22_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT20646.1"
                     /translation="DYADDNDNLQKYYNYKYTKPSFFLNVHRDLTQNLKILVGIEVKK
                     VDIEKWDGYKFDHDKQGPTLLKQWQPEGYDGGWSNFVRAGIYYDTRDYEPDPKRGYLI
                     DYAYEISDNVLGSDYDFMRNTVQLQGYIPLLSSLTLALRVAYTDTNSDTPFFEMGYFG
                     FSLNRRTGLGNNRTLRGYREQRFVGPTMTVGNAELRWKFAETNAWGQNFQFKLTGFYD
                     VGNVYDEAGDPFSDPRFSDYHQGYGGGLVIAWNMATIVHFYYGTSEEDTSISVDFSHT
                     F"
BASE COUNT          991 a          541 c          612 g          897 t
ORIGIN      
        1 gccagcggta taaacattgg taaaaatagt gagcccaatc ctacaagatg gaatatgata
       61 ggaagagcta cagcacaggc agccgtaatt gatgcgatta taatctgcgt tattgtaatc
      121 tcttcatgca taaatataaa ttatagatac tattttatga ttaaaaatat attgtttgta
      181 atactcaatt gatggtgatt tatttcaagc agtatttttt gttgtgataa taaaattttt
      241 cttgaacata atgatgttta tgatagtatt gaatttagag gttgaaccac tgaaccacta
      301 aaaaggagaa tatagtatgt caaaaaaatt tatacccgat tggaccgaaa aagctccaga
      361 gccctattca tatcgttcta ttttcaaatg gggcgatcca cattcattta aacatcccaa
      421 tccaaaatta tatcaagaat taaaaacggt gtttaatctt gacgatagct attttgcaaa
      481 aaaacacagc gaggggagaa atacagttgc atataaaaaa cctgtaaggc tgacaaaaaa
      541 gcaattacag gatattgccg atattgcagg ctatgaaaat ataagcacaa cgcagtttga
      601 tagattaaaa tttgcaacag gtaaaaccat agaagaagct atgaaattac gtaaaggtat
      661 tgttgaacga gttgccgatt gtgttgtcca tccacgcagt gttgatgatg ttgaaaagct
      721 tatacagtat tgccatagac ataaaatacc ggtatatgtt tatggcggtg gctcttcggt
      781 taattttgga ctgcgaactg caaaaggtgg cattactctg gtactatcca cccatatgaa
      841 taacatacta catgttaatg aaaaaaatca aacggttact gtacaggctg gtatacaggg
      901 acctgctctg gaagaagcat tgaacaatgc taaaaagcat tttggtacaa agtataatta
      961 tacctgcggg cattttccac aatcgtttga atattcatcc attgggggct ggattgtaac
     1021 attgggttcg gggcagtcat caagctatta tggtgatgcc tatgatcttg ttataagcat
     1081 gaagtgggtt acccctgctg gtacatttac aaccagggat tatgctgcaa cagcaaccgg
     1141 tcctaaaata aacgatatca tgaaagggag tgaaggcacc tttggcgttt tagtggaggt
     1201 aacatggaag atatatcgtt atatgccaca aaatagaaga agatttggtt ttatttttaa
     1261 aacatttcag gatgccatca atgcttcgcg tgaaatatcg cagggtgagt ttggtatgcc
     1321 agcagtattc agggtttcag atgaagaaga aacgcatata ggtttgaaac tctatggcat
     1381 agatggaacc attctggata aactaatgca gctacgcggt tttaaaccaa tgcagcgttg
     1441 cctttttata agctcagcag aaggcgaaaa gcattttacc aaaaacataa aacgtcaggt
     1501 aaagaaaatt tgtaaaaaat acggagggat gtacattaca ggattgccaa cgcttatgtg
     1561 ggagcccgga cgatataagg acccttatat acgagaggat ttgcatgatt acgggatact
     1621 cattgataca ctggaaacaa gtgtaaagtg ggataacctc catgatgttc ataaggctgt
     1681 gcgacagttt ataaaaaaca ggcctcagac agtatgtatg actcatgctt cgcattttta
     1741 tcctcagggt acaaacctgt attttatcta tattatgaaa actgatgata tgaacgaata
     1801 cattacattt caggatggta ttatcgatag catagcaaaa agtggtggat cattgagcca
     1861 tcatcatggt gtagggcgaa tgttagcacc atggatggaa gagcatattg gcaaagaaca
     1921 gatggcagtt ttacgagcaa taaagaagca ttttgatccc aatgctatta tgaatccggg
     1981 tggacagctg ggattggatt taaaaggcaa agattggcgc tctataaagt aatatacatc
     2041 gtatgcaata aagggcagtg gtatacttcc ctttattgta ataatagatg aatagcactg
     2101 gaatcctttg ctatagaata aagtaaattt tggcgatagt tcatattaat taaaaggggc
     2161 aacgtaaaca gttgcccctt tttgtgtata ttttaaatct attactatta aaatgtatgg
     2221 ctaaaatcaa ccgatattga tgtatcctct tcacttgtac cataatagaa atgaactatg
     2281 gttgccatat tccatgctat gaccaaaccg ccaccataac cctgatgata gtcgctaaaa
     2341 cggggatcac tgaatggatc gcctgcctca tcgtatacat ttcctacatc ataaaatccc
     2401 gttaatttaa actggaagtt ctgaccccat gcattcgttt cggcaaattt ccagcgcagt
     2461 tcagcattcc ctaccgtcat tgttggccca acaaaacgtt gctcgcgata tccccgtaag
     2521 gtcctgttgt tgccaagccc tgttctgcgg ttgagcgaaa atccaaagta acccatctca
     2581 aagaatgggg tgtcactgtt ggtatcagta taggctacac gcaatgccag cgttaaactt
     2641 gaaagcaatg gtatataacc ctgtaattgc acggtgttac gcataaagtc ataatctgag
     2701 ccaagtacat tatcagatat ttcatacgca taatcaatca agtatcctct tttggggtca
     2761 ggttcataat cacgggtatc gtagtaaata ccagcacgaa caaagttgga ccatccacca
     2821 tcgtatccct ctggttgcca ttgtttcaat aatgttggcc cctgtttatc gtggtcaaat
     2881 ttatagccat cccatttttc tatatctact tttttaacct caatccctac cagtatcttc
     2941 aggttttgtg ttaaatccct gtgaacattc aggaaaaagc tgggcttggt atacttatag
     3001 ttgtaatatt tttgcaaatt atcattatca tcagcataat c
//
LOCUS       DAOSOX010000251         3010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_32718_length_3010_cov_7.898139, whole genome shotgun sequence.
ACCESSION   DAOSOX010000251 DAOSOX010000000
VERSION     DAOSOX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32718_length_3010_cov_7.898139"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..668)
                     /locus_tag="PLX22_11925"
     CDS             complement(33..668)
                     /locus_tag="PLX22_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOT20647.1"
                     /translation="MLYKARKSVNADAGSIYIKVNDELVFSHVQNDTLSRYLPHGQKL
                     IYTTFRIKINMRSIAGFVAATGLIVNISNVNNIAPNLLYKFNSTYDDISHYKTTSMLT
                     VPLQTINGTIIGILQLINAIDANTNIIPFTKEDEPIVMLFANNASIVLQRAQLTRALL
                     LRMIRMAELRDPKETASHVNRVATYAIEIYERWAKRYKLNEKELLKLSSLQ"
     gene            1167..2897
                     /locus_tag="PLX22_11930"
     CDS             1167..2897
                     /locus_tag="PLX22_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOT20648.1"
                     /translation="MKLTVFYFDPTVNSMFVEKTDETGNTFYTLSSPYNFVYRDEIVA
                     RVIDVDDPSDVVYHLDTGYKYYKTVKFKPFKAGDGIIYDTISGCYRATDYGFVTYDQR
                     TSRINLKLPLQVKKDKLKAFYIIFPTKFLKIPTYKDIEQQLLAHKIIGILDKTEIESQ
                     LNGIDINNPKVHRILVARGKEPMNGYDDYYVSLIPIEKKAGKLLDDGRIDFKEVGTII
                     EIKKGQDVLQRIPGIKPEDGFDIYGEKVEATIEQKEGYYPGKNIVPSSDDPSIYISQI
                     DGCLDIDNKNLSVLPVAIIKGDVNYESGNIDFDGSVQIMGSILPGFTVNATGDVYVDK
                     NIDDAKVTANGDIIVKLGIGGKGDSKIIAGGNVKAKFILNSYLEALGKIEIEDSIINS
                     TAFSNDDVVVSGRHGKIIGGETIARHQIVVNVAGSQMQNITKLTAGKSLIVERELADL
                     RKQMGKYKSNVDDLLLKIKNSFGEGVFENPKEFLNSLPPIKKKNCLMLLKELSENNHQ
                     LKLLLDQRMEIENKYKLEKEPSISIIDIAYPGVIISIKKSKMQLEKSMQNTKFYEDAE
                     TKRITFTAAV"
BASE COUNT         1046 a          409 c          539 g         1016 t
ORIGIN      
        1 agcactcaat gctatacaaa tagctgagtt gcttattgca aagaagatag tttaagtagt
       61 tctttctcat ttagtttata tcgtttcgcc caacgttcat agatttcaat agcataggtc
      121 gcaactctgt taacatggga agctgtttct ttaggatcgc gcaattcagc cattctgatc
      181 atcctcagta gtaatgcacg agtcaattgt gcgcgttgta atacaatact ggcattattt
      241 gcaaagagca ttacaatcgg ttcatcttct ttagtgaaag gaataatatt agtgtttgca
      301 tctattgcat ttattaactg cagtatacca atgatagttc cattgatggt ttgtagtgga
      361 actgtcagca tcgaagtggt tttataatgc gaaatatcat cataggttga gttaaattta
      421 tatagcagat tgggagcaat attgtttacg ttcgaaatat ttactattaa tccggttgct
      481 gcaacaaaac cagcgataga acgcatgttg atttttatcc tgaatgttgt gtaaatcagt
      541 ttttgtccat ggggcaggta tcttgaaagt gtatcgtttt ggacatggct gaaaacaagc
      601 tcatcattaa ctttaatgta tattgagcca gcatctgcat tcactgactt gcgagccttg
      661 tacagaattt tttcaagtag tatatctgaa ccctgaatag tatttaattc attagaaagt
      721 tctattattt cattaattga taatctggtg ttttgtcata atatcccatt tcgatacata
      781 cctataattc agttatagta caatacaaaa tattggaagt aaagtctaca atattacagt
      841 aaataatgca ttcctaaaat tcatttttac aggataaatg tattatcaga ttatgctatt
      901 tattaaatga gtattacaaa ttcatgatcg ctctcaatat aggcaaaaat cgatagaact
      961 tatccgtcct tatagtatta attcatattg agagcaacat tttttagtga tgttaataaa
     1021 ttataataaa tgtttataca atattttact tgtgtttcat aatatgatga tgtataatta
     1081 atgttgtatt gccgatagtt aattataaga ggtgtaattt ctgtgaataa taatagcagt
     1141 tgaataatta tcattagcga ggattgatga aattaaccgt tttctatttt gatccaactg
     1201 taaattcaat gtttgttgaa aaaaccgacg aaacaggcaa tacattttat acattatctt
     1261 ctccctataa ttttgtttat cgcgatgaaa ttgttgcacg tgttattgat gttgatgacc
     1321 cttccgatgt tgtttatcat ctggatactg gttataaata ttataaaact gttaaattta
     1381 aaccatttaa agctggtgat ggcataattt atgatacaat tagcggttgc tatcgtgcaa
     1441 cagattatgg ttttgtaacg tatgatcaga ggacttcacg cattaatcta aaattacctc
     1501 ttcaggtgaa gaaagataaa ttaaaagcat tctatattat tttccctaca aaatttctaa
     1561 aaatacctac ttacaaagat attgaacagc aacttttagc tcataaaata ataggtattt
     1621 tagataaaac cgaaatagaa tcacagctta atggaataga tatcaataat cctaaggttc
     1681 accgtattct tgttgctcgt ggtaaagagc caatgaatgg ttatgacgat tattatgttt
     1741 cattaattcc gattgaaaaa aaagccggaa aattattgga tgatgggcga atagatttta
     1801 aagaagttgg tacgattatt gagataaaaa agggtcagga tgttttacaa cgtattcctg
     1861 gtataaaacc tgaagatggt ttcgacatct atggcgagaa ggttgaagca accatagaac
     1921 aaaaagaagg ttattatcct ggtaaaaata ttgtgccctc cagtgatgat ccatcaatat
     1981 acatttctca gatcgacgga tgtcttgata ttgataataa aaatttatct gtattacctg
     2041 tagcaataat taagggtgat gtaaattatg aatctggcaa tattgatttt gatggttcgg
     2101 tgcaaattat gggatctata ctgccaggtt ttactgtcaa tgcaacgggc gatgtttatg
     2161 ttgataagaa tattgatgat gccaaagtaa cagctaatgg tgatattatt gtaaaattag
     2221 gcataggtgg gaaaggcgat agtaaaataa tagcgggtgg taatgttaaa gctaaattta
     2281 tattaaattc gtatcttgaa gctttaggga agattgaaat tgaagattca attataaata
     2341 gcactgcatt ttctaatgat gatgttgttg tatcagggag gcacggaaaa attatagggg
     2401 gcgaaactat agcacgtcat cagatagttg ttaatgttgc aggttcgcaa atgcaaaata
     2461 ttacaaagtt aacagcaggt aaaagtttga tagtggaacg tgaattggct gatttgcgta
     2521 aacagatggg aaagtataaa tcaaatgtag atgacttgtt attaaagata aaaaatagtt
     2581 ttggtgaagg tgtatttgaa aatccaaaag agtttttaaa tagtcttcca ccaattaaaa
     2641 agaaaaattg tttaatgtta ttaaaagaac tatcagaaaa caaccatcaa ttaaaattgc
     2701 tgttagatca gcgcatggag attgaaaata aatataaatt agaaaaggaa ccatcaataa
     2761 gcatcattga cattgcttat cctggtgtta ttatatcaat taagaaaagc aaaatgcagc
     2821 ttgaaaaatc aatgcaaaat actaaatttt atgaagatgc cgaaacaaaa aggattacat
     2881 ttacggctgc cgtgtaaatt acacttaaat taacaaatct ctcattcaat gagctatgac
     2941 cattacattt tatcctcttg acatcataat aattatgcta tgcgatagtt atcggtacta
     3001 tatacactac
//
LOCUS       DAOSOX010000252         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_32847_length_3001_cov_8.079769, whole genome shotgun sequence.
ACCESSION   DAOSOX010000252 DAOSOX010000000
VERSION     DAOSOX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32847_length_3001_cov_8.079769"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLX22_11935"
     CDS             <1..736
                     /locus_tag="PLX22_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT20649.1"
                     /translation="KIAIVTGASRGIGQSIAKGLAAHGATVFCTSRKIEDLNKVKDEI
                     ELEGGKAHAIACHIGKLDDIQNLFKAVEERYGRLDILVNNAATNPYFGDVLHATADAW
                     DKTIDVNFKGYFFMSQYAANLMIKNKGGSIVNVASINGIRPAPFQGIYSMTKAGVIAM
                     TKSFAKELAPFNVRVNAILPGLTDTKFSSVMTQNEEILKLILPTIPMKRVANPDEMVG
                     AVVYLVSDAASYTTGATIVIDGGALA"
     gene            764..2035
                     /locus_tag="PLX22_11940"
     CDS             764..2035
                     /locus_tag="PLX22_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20650.1"
                     /translation="MATITMNNETDTIPFDQMQMQINKATEDVSVKCNTAIEPYIGFE
                     KGCTRISIPVPELSPALIIQSLGVIKDHIEHIRIHSFSEGYYAVQALNKNIFKTENIF
                     DNCKIEFSGLMTNFKIECTKKGNLSQEEINCIIALMQKAYSSMKENPVTRLIRRGASV
                     YTGTEGIDWNYIAGYEEVKRIIRESIILPLKNPSVYDNIARLTRKTFEYNKPKAILFE
                     GPPGVGKTTIARIIAREVQVPLIYVPVESIMSKWYGQSSQNLAEIFDASEDMGGSILF
                     IDEIDSLAGSRDQNMYEATRRILSVLLRRLDGIGAIANTMVIGATNRKQDLDHALINR
                     FDQSIHFPLPGENERASIFGNYAQHLNPEELMKLAHISNTLSGRTIKDLCEQTERRWA
                     RKLIIKNLPPSQPPFEYYYHTVKNFFTTQSG"
     gene            complement(2016..>3001)
                     /locus_tag="PLX22_11945"
     CDS             complement(2016..>3001)
                     /locus_tag="PLX22_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOT20651.1"
                     /translation="GFWLPELGYCYGMDSYLKDENIAYTIVNDKSILLSNAVPACGNF
                     SPSMTKSGLMVLPRDSVLSMKIWSGQGYPGNPYYREFHRDALYELQELSPTPDNTLLG
                     LKIYAVSGSDDKEYYNPDCIPEIVSKQVDDFIDAIYKRSEQVANITHKSPVFILPFDA
                     ELFGHWWFEGPLFLELLIEKITQLHGVELIMPSDLLGSECATVDLVESSWGKQADFST
                     WYNPLVRRNAAMLDEMIYEFLQYVNVKNEMLYQCAREILLAMSSDWLFMISTGSYTDY
                     AGRRFSEHSDAARTLLTMLKNHESNETLIHERYKKYPIFPSIDRVLTRIEL"
BASE COUNT          966 a          618 c          595 g          822 t
ORIGIN      
        1 caaaatagct attgttaccg gagcaagccg tggtattgga cagtcaatag caaaaggttt
       61 agcagcgcat ggtgcaacag ttttttgtac cagcagaaaa atagaagatt taaataaggt
      121 aaaagatgaa attgaattag aaggcggtaa ggcacatgcc atagcctgtc acatcgggaa
      181 gcttgatgat atacagaatc tttttaaagc tgttgaagaa cgctatggta gattggatat
      241 actggttaac aatgcagcaa ccaatccata ttttggcgat gtattacatg caacggctga
      301 tgcatgggat aagaccattg atgttaactt taaagggtac tttttcatga gccagtatgc
      361 tgcaaatctg atgataaaaa ataagggggg ctccattgtc aatgttgctt caatcaatgg
      421 tattcgtcct gcaccatttc aggggattta ttctatgaca aaggcaggtg ttattgccat
      481 gacaaaatcg tttgctaaag aactggcacc ttttaatgtg cgcgtcaatg caatcttacc
      541 gggtttaacc gatacaaaat tttcttcagt tatgacacaa aatgaagaaa ttttaaaact
      601 cattctgccg acaataccaa tgaagcgagt agctaaccct gatgagatgg tgggagcggt
      661 agtatatctt gtatccgacg cagcaagtta tacaacaggg gctactattg ttatagatgg
      721 gggagcgctt gcataatttt tgaagtttta tttactggta tatatggcaa ccataaccat
      781 gaacaatgaa acagatacca tcccatttga ccagatgcag atgcagataa acaaagccac
      841 tgaggatgtg tcggttaaat gcaacactgc aattgagcca tatattggtt ttgaaaaagg
      901 atgtacgcgc atcagtatac ctgttccgga gctatcgcct gcactcataa tacaaagcct
      961 gggtgttatc aaagatcata ttgaacatat acgcatccac tctttttctg aagggtacta
     1021 tgctgtgcag gcattaaata agaatatatt taaaaccgaa aacatctttg acaactgtaa
     1081 gattgaattc tcagggctta tgacaaattt taagatagaa tgtacaaaaa aaggcaatct
     1141 atcgcaggaa gagattaact gcatcattgc acttatgcaa aaagcatatt cgtcaatgaa
     1201 agaaaatccg gtaacacggc tcatacgacg gggtgcatcc gtatataccg gtacagaagg
     1261 tattgactgg aattacattg caggatatga agaggtaaaa cgtatcatac gtgaatcaat
     1321 aatactccct ttaaaaaatc cttcagtata cgacaacatt gcccggttga cacgcaaaac
     1381 atttgaatac aataaaccaa aagcaatact ttttgaaggg cctcccggtg ttggcaaaac
     1441 caccatagca cgtatcattg ccagagaagt acaggtgcct ctcatctatg ttccagttga
     1501 atcaataatg tcaaaatggt acggacagtc atcgcaaaat ttagccgaga tatttgatgc
     1561 aagcgaagat atgggaggat caatcctttt cattgacgag attgattcac tggcaggctc
     1621 tcgtgaccag aacatgtatg aagcaacccg tcgcatttta tcggtgcttt tacggcgtct
     1681 tgatggtata ggagctatcg ccaatactat ggttattggt gcaacaaatc gaaaacagga
     1741 tttagatcat gcgcttataa accgatttga tcagagcatt cactttcccc ttcccggcga
     1801 aaatgagcgc gcaagcattt ttggaaacta tgcacagcat cttaaccctg aagagcttat
     1861 gaagttagcg catataagca atacactttc cggaagaacc atcaaagatc tatgcgagca
     1921 gaccgagcgc agatgggcac gaaagctcat tataaaaaat ttacccccct cacagccgcc
     1981 atttgaatat tactatcaca ccgttaaaaa ttttttcaca actcaatccg ggtaagtact
     2041 ctgtcaatag aggggaatat tggatacttt ttataccgtt catgaatcaa tgtttcatta
     2101 ctttcatgat tttttagcat cgtaagcagt gttcgcgcag catcgctatg ttcagaaaat
     2161 cgcctgccag cataatcagt gtagctgcct gttgatatca taaacaacca gtcagatgac
     2221 atagccagta aaatctcacg ggcacattga tacagcatct catttttcac gttcacatat
     2281 tgtaaaaatt catagatcat ctcatcaagc atggcagcat tcctgcgcac cagcggattg
     2341 taccatgttg aaaagtctgc ctgttttccc cacgatgact caactaaatc aactgtcgca
     2401 cattcactgc ccaacaaatc agaaggcata atcagctcta caccatgtaa ttgtgtaatc
     2461 ttctctatga gcaattccag aaaaagtggc ccttcaaacc accagtgtcc aaaaagctcg
     2521 gcatcaaatg gaagaataaa aacaggggat ttgtgggtaa tgtttgcaac ctgctctgac
     2581 cgcttataga ttgcatcaat aaaatcatct acctgtttac ttactatctc aggaatacag
     2641 tctggattat aatattcctt atcatcactt ccgctaactg cataaatctt aagccctaaa
     2701 agggtattat caggtgtggg cgatagctct tgtaattcat agagtgcatc cctatgaaat
     2761 tcccggtagt atggattgcc agggtatccc tgccctgacc atatcttcat tgaaagaaca
     2821 gaatcacgtg gcagtaccat caatccgctt tttgtcattg atggggaaaa attcccacac
     2881 gctggaacag cattgctcag caaaatgctt ttatcattaa caatggtata agcaatgttt
     2941 tcatccttca aatagctatc catgccgtaa caatagccca gctcgggcaa ccagaagcca
     3001 t
//
LOCUS       DAOSOX010000253         3000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_32859_length_3000_cov_7.821392, whole genome shotgun sequence.
ACCESSION   DAOSOX010000253 DAOSOX010000000
VERSION     DAOSOX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32859_length_3000_cov_7.821392"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..636
                     /locus_tag="PLX22_11950"
     CDS             229..636
                     /locus_tag="PLX22_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOT20652.1"
                     /translation="MISFDLQCANGHLFEGYFKDYDAFDKQLNAGLVECPLCNSREIK
                     RLFTGCSIQTKNSFSDKKQPSFFEMIRMVESYIKQHFENVGNEFPEIARAIYYGIEEE
                     RNIYGTASREDMEELLDEGIPILPVPKIDDVEQ"
     gene            complement(644..1126)
                     /locus_tag="PLX22_11955"
     CDS             complement(644..1126)
                     /locus_tag="PLX22_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20653.1"
                     /translation="MMDAVIDFFAKILSYCVQFINRVADLLLRLYEYCIGIFRELEIF
                     EKCIIIVSIVSLVIPVLPIARFYIFESWYYVNNPLAIYFIGIIIIMVVSALWQKFWIL
                     LARLLIILYYCAWIIYLPIGNCITKARPYELAYGYYCNIVVSVVYIVLCVLSLINSRR
                     "
     gene            1500..1572
                     /locus_tag="PLX22_11960"
     tRNA            1500..1572
                     /locus_tag="PLX22_11960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1533..1535,aa:Ala,seq:cgc)
     gene            complement(1686..>3000)
                     /locus_tag="PLX22_11965"
     CDS             complement(1686..>3000)
                     /locus_tag="PLX22_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT20654.1"
                     /translation="FCKHPTQTNNTIVYYQSATGYKEFLKEQSLNYVSRFTDTGAVIS
                     DAFEESVKDTVIALAYISPEKGYFNAMLDAPFATSCNDYINTVKDYKGQPRIFVVQDG
                     VTSFQIIAGNTITDNDSPVLATPQKALIAKTVANLFDVQQMDMLVAGSEMIENPSFAV
                     QKMMAFNNLMRLKTIQDAMSQVAIYRNNDVASLLTDIKYPAAQQYLSIMNNLLGKMPI
                     TSAKLANIYFQEWNFTMDKDYVAPTLYNAIITSLVKETFTDELPYTVGDMLQYYMIFE
                     PEFLRIFQEDKSPYFDDTATVGKLESRDLIFDRAFLHSLNYFNYTIGPYIDTWRWGKV
                     NRGIVDDKTQGSNVVNRVKKKKTISLSGFDNTVFRNLIKRQDYSIDSITVCASYMDEN
                     THRMTALISPVMDVMPSYYTDINANTFFTINVNNKHDLFTINPVK"
BASE COUNT          976 a          565 c          502 g          957 t
ORIGIN      
        1 gtattcctca tttttacttt ttgatttttt tgccatggtt tagagttatg gcgatacaaa
       61 tgatgtactt tgctactatt ttatataact atctttcttg acattatgca actgcccaat
      121 caacttttga ataattgcct ctatcacaat tatatatttt ttttgaaatg taattgaaaa
      181 actattacaa ttgttgccag atagttatta ttaatgaagt gagttgatat gataagcttt
      241 gatttacaat gtgccaatgg ccacctattt gaaggatatt ttaaagatta tgatgcgttt
      301 gacaaacaat tgaatgctgg tcttgtggaa tgtcctttat gcaattcgcg tgaaattaaa
      361 agacttttca ccggatgttc catacaaaca aaaaattcat tttcagataa aaaacagcca
      421 tctttttttg agatgatacg gatggtcgaa tcatacataa aacaacattt tgaaaatgtt
      481 ggtaatgagt tccccgagat tgcccgtgcc atatactatg gcattgaaga ggaacgaaac
      541 atttatggaa ctgcatcccg tgaagatatg gaagagctcc ttgacgaagg aataccaatc
      601 ctgcctgtgc caaaaattga cgatgtagaa caatgaaggg ttgctatcgc ctgctattaa
      661 taagcgatag cacacataaa actatataga caactgaaac aacaatatta cagtaatatc
      721 catatgccag ctcatatggt cgcgcttttg tgatacaatt gccaataggg agatagatta
      781 tccatgcaca gtaatacagt attatgagta gtcgtgccag taaaatccag aatttttgcc
      841 atagagcaga aacaaccatg ataatgataa tcccgataaa gtatatggca agcggattat
      901 tgacataata ccagctttca aagatataaa aacgcgctat aggcaaaacg gggataacca
      961 gagaaacaat tgatactata ataatacatt tttcaaaaat ttcaagctcg cggaagatac
     1021 caatgcaata ttcatacaat cgcagaagaa gatcagcaac ccgattaata aactgaacac
     1081 aatatgaaag tatttttgca aaaaaatcta ttaccgcatc catcatattt ctcctcttgc
     1141 aattctggta gttgtactac caaatgttag tatccataaa aaaatacaat gatatttcat
     1201 tatctaccgg atggcctttc attttttaaa agttctgcaa acaattcatc aattcataac
     1261 gatactatcc gcaacaatga aacggattta tacgaaatat ttccattgga aacgcatagt
     1321 tattataagc atactttcat aaaaattgct ctatgataag gttatgaaaa tattttttgt
     1381 cttcaccagg agctgtggca ccatgatgaa atccttttat acccatacct gccactaaaa
     1441 aatctattga cgaaaaacat gatttaccct tccatgatta atatactata tattcttttg
     1501 gggctgtagc tcagctggga gagcgctaga atcgcactct agaggccgag ggttcgactc
     1561 ccttcagctc cacaggcata gtataaatat tttttgtcct ttcattgcgc ttcatgcatg
     1621 cacagctcaa tacatactca aacaacatct catactgcaa ccgtgttgtg tgcctctacc
     1681 aaaatttatt taacagggtt aatggtaaaa agatcgtgct tattattgac attgatggta
     1741 aaaaaagtat tggcattaat atcggtataa taggatggca tcacatccat aacgggacta
     1801 atcaatgctg tcatccggtg tgtgttttca tccatatacg atgcacatac agtaatgcta
     1861 tcgatactat aatcctgcct tttgatcaaa ttcctgaaaa cagtattatc aaagccgctt
     1921 aatgatatgg tcttcttttt cttcactctg ttcactacat ttgaaccctg cgttttatca
     1981 tcaactatcc ccctattaac tttaccccat cgccatgtat caatatatgg tccaatagta
     2041 tagttaaaat aatttaaaga atgcaaaaac gccctgtcaa agataaggtc acgtgattcc
     2101 agttttccca cagtagcagt atcatcaaaa tagggtgatt tatcctcctg aaaaatcctt
     2161 aaaaattctg gttcaaagat catataatac tgcaacatat cacctacagt atacgggagt
     2221 tcatcggtaa atgtttcttt aacaagcgat gttatgatgg cattatacaa tgtaggcgct
     2281 acatagtctt tgtccatggt aaaattccac tcctgaaaat aaatgtttgc caatttagcc
     2341 gatgtaatgg gcatttttcc cagaagattg ttcattatag acaggtattg ctgtgcagca
     2401 ggatatttaa tatcagtaag caatgatgca acatcattat tccggtagat agcaacctgt
     2461 gacatggcat cctgtatagt ttttaatcgc atcagattat taaatgccat cattttctgg
     2521 acagcaaatg atggattctc tatcatttca ctgcctgcta caagcatatc catctgttga
     2581 acatcaaaaa ggttggctac cgtttttgca atcagcgctt tttgtggtgt tgcaagcaca
     2641 gggctatcat tatctgttat ggtattgcct gcgattatct gaaaagaggt caccccatcc
     2701 tgaactacaa aaattcttgg ttgaccctta tagtctttta cagtattgat ataatcattg
     2761 caggacgtcg caaatggtgc atcaagcatt gcattaaaat atcccttctc tggacttata
     2821 taggcaagtg caataacggt atcttttact gattcttcaa atgcatccga tataaccgcg
     2881 ccagtatcag taaatcgtga cacatagttt aatgattgtt ctttcaaaaa ctctttatag
     2941 cctgttgcac tttgataata aacaatggta ttgtttgttt gcgtggggtg cttgcaaaaa
//
LOCUS       DAOSOX010000254         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_33920_length_2926_cov_8.629397, whole genome shotgun sequence.
ACCESSION   DAOSOX010000254 DAOSOX010000000
VERSION     DAOSOX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33920_length_2926_cov_8.629397"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLX22_11970"
     CDS             <1..186
                     /locus_tag="PLX22_11970"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT20655.1"
                     /translation="ILDRITGKPGVVDFIYIGDDAVFRWPAFNVADSSIVIGATLLIL
                     HQIIEEKKRKSQKGHHQ"
     gene            complement(180..578)
                     /locus_tag="PLX22_11975"
     CDS             complement(180..578)
                     /locus_tag="PLX22_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20656.1"
                     /translation="MFRGHYCRVLVRCLFIIVLVIVSCGKTPPLQFCEGVTDEGTPVN
                     CGSQFTTGDMTLYVTAKEAFGSTTATVVIYEKKHYSQPEYTRFDYTINPDVSSTIIPL
                     SLYAEGTYIVTVIVGDKEIASGTISIVDTY"
     gene            complement(556..843)
                     /gene="xseB"
                     /locus_tag="PLX22_11980"
     CDS             complement(556..843)
                     /gene="xseB"
                     /locus_tag="PLX22_11980"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT20657.1"
                     /translation="MEKKAKKPTFEEALEELETIAQKLESGELTLDESIQYYEKGVAL
                     AKFCHQKLEEAERKIEILQKQPDGSIQKKSIKVKEDTGEIEDDEDVQGSLL"
     gene            complement(827..2131)
                     /gene="xseA"
                     /locus_tag="PLX22_11985"
     CDS             complement(827..2131)
                     /gene="xseA"
                     /locus_tag="PLX22_11985"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT20658.1"
                     /translation="MEDIEKHETVYSVSDITGIIKQLVASAPELNGVWIKGEISNLTY
                     HSSGHIYFSLKDEGAVIQAAFFKYSNKNLDFRLEEGMSVVVFGGVTVFEKRGQYQFIV
                     YKMRKEGLGELLQRIEKLKEKLYKEGLFDPRRKKPLPQLPRRIGIVTSPTGAAIRDII
                     KVAMRRFPNLEIVIAPAKVQGEGAAESIVAGIQELNKPVWGIDVIIAGRGGGSFEDLM
                     AFNEEKVVRAFAASKLPIISAVGHQIDSPLSDLAADVAATTPSAAAELAVPVKDDIQN
                     TIEQIIGKMTKRMMMVLQTFNNRIALALQYRAFQNPMSMLEQHELALSDIENRISLSI
                     HKRLYDARESLNRIKDLHLLVKNRLQNYKHVFMMNASKVEHLSPVAVLKRGYSITLKG
                     NTSVASVQGIRQGDEVKVIVYDGSMNCEVQSTSNEVNFGKKS"
     gene            complement(2142..>2926)
                     /locus_tag="PLX22_11990"
     CDS             complement(2142..>2926)
                     /locus_tag="PLX22_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOT20659.1"
                     /translation="VDAAATMAKIAQYAEDSIMYKATMEERKIYTRAIITDAIALSAS
                     ESAKTLGADCIITATQSGYTARKISKYKPQIPVIAVTTNQKVVNQLSLSYGVVPVQIK
                     HCEDINTLVDEALNTCKQKELIHNGDLLIITAGILAGVTGGTNVLRVYLVANEIARGI
                     GYGDTVVKGYIRKKTVSGELSHNGIIITDDIEQLKRSPLPLAVITSQSTIASPIIHFC
                     REHGIPVIGGIAIDTLPCTDGDDVTLDILRGTIYRGNVHLPF"
BASE COUNT          776 a          697 c          519 g          934 t
ORIGIN      
        1 atcctggaca gaattaccgg caaacctggc gttgttgatt ttatctatat tggtgatgat
       61 gctgtctttc gctggcctgc ctttaatgtt gctgattcat cgatagtcat aggtgctacc
      121 ttgcttatac tgcatcaaat tatagaagag aaaaagcgta aatcgcaaaa aggtcatcat
      181 caataagtat caacaatgct gatagttcct gaagctatct ctttgtcacc aactatcact
      241 gtaacaatat aggtgccttc tgcatatagc gaaagcggga taatggtgct ggatacatca
      301 ggattgattg tataatcaaa tcgggtatat tcgggctgac tatagtgttt tttttcatat
      361 ataacaacgg tagcggtggt gctgccaaaa gcttcctttg cagtaacata caatgtcata
      421 tcaccggtgg taaactggct gccgcagttg accggcgtac cttcatcggt aaccccttca
      481 caaaattgca atggcggcgt tttcccacac gatactatca ccagtactat tatgaataaa
      541 caccgtacca atactctaca ataatgaccc ctgaacatcc tcatcatcct ctatctctcc
      601 cgtgtcttct tttaccttta ttgatttttt ttgtatgctt ccatcgggct gtttttgcaa
      661 aatctctatc tttcgctcag cttcttctaa tttctggtgg caaaattttg ccagcgcaac
      721 acctttttca taatactgta tggattcatc aagggttaat tcgcccgatt ctaacttttg
      781 ggctattgtt tcaagctcct ccagcgcctc ttcaaatgtt ggctttttag cttttttttc
      841 caaaattcac ctcgtttgat gttgattgta cctcacagtt catagaacca tcgtatacaa
      901 tgacctttac ctcatccccc tgccgtatcc cttgaactga ggctacactg gtattgcctt
      961 tcagggtaat ggaatagccg cgctttaaaa ccgccaccgg tgataggtgc tcaaccttgc
     1021 ttgcattcat cataaataca tgcttatagt tttgcaatct attttttaca agcagatgca
     1081 gatcctttat cctgtttaat gattcccttg catcgtacag gcgcttatgg attgacaatg
     1141 aaatcctgtt ctcaatatca gacagcgcaa gctcatgctg ctccagcata ctcatgggat
     1201 tttgaaacgc acgatattgc aatgccagcg caattcggtt attaaatgtt tgtaatacca
     1261 tcatcattcg cttggtcatc tttcctataa tttgttcgat agtattctgg atatcatcct
     1321 tcaccggaac agcaagctct gctgctgccg aaggggttgt tgccgcaaca tccgcagcaa
     1381 ggtcgctcag cgggctatca atctgatgcc caactgccga gatgatggga agctttgatg
     1441 ctgcaaacgc acgcacaacc ttctcttcat tgaatgccat caaatcttca aatgaaccgc
     1501 caccccttcc tgcaatgata acgtcaatac cccacacggg tttgttcaac tcctgtatac
     1561 ctgcaacaat agattcggcg gctccctctc cctgaacctt agccggagca atgacaattt
     1621 caaggttagg gaagcgcctc atcgcaacct tgataatatc gcggattgca gctcccgttg
     1681 gcgaggttac aattccaatg cgtctgggaa gttgtggtaa tggttttttc cgccggggat
     1741 caaacaatcc ttctttatat aatttttcct ttagcttttc aatgcgctgc aaaagctctc
     1801 caagtccttc ctttctcatt ttatatacta taaattgata ctgccctcgc ttttcaaata
     1861 ctgtcacacc accaaaaaca acaacgctca tcccttcttc taatctaaag tctaaatttt
     1921 tattggaata tttaaaaaaa gctgcctgaa tcactgcacc ttcatccttc aacgaaaagt
     1981 agatgtgccc cgatgaatga taagtaagat ttgaaatctc accttttatc catacaccat
     2041 tgagctcagg cgctgatgca accagctgtt ttataatgcc ggtaatatcg cttaccgaat
     2101 acaccgtttc atgtttttca atgtcttcca tacatttcta cttaaaaggg taaatgaaca
     2161 ttgccacgat agatagtacc tcgtaaaata tcaagcgtaa catcatcacc atcagtacat
     2221 ggtagcgtat ctatagcaat tccacctatc accgggatgc catgttcccg acaaaaatga
     2281 ataatgggtg atgctatagt actctggctg gtaataaccg ctaacggcaa tggtgatctt
     2341 tttaactgct caatatcatc ggttatgatg ataccattgt gcgatagctc tcctgataca
     2401 gttttttttc gtatatatcc ttttaccacg gtatcaccat agccaatacc acgagctatt
     2461 tcatttgcaa caagatacac tcgcaataca tttgtcccac cggttactcc cgctaaaata
     2521 cccgcagtta taatcagcaa atccccatta tgaataagct ctttttgttt acatgtatta
     2581 agagcttcat caacaagtgt attgatatct tcacagtgtt taatctgcac cggaacaact
     2641 ccatacgaca acgataactg attcaccact ttttggtttg ttgttaccgc tataaccggt
     2701 atctgaggtt tgtatttgga tatctttcga gcagtatacc ctgattgcgt ggcagtaatt
     2761 atacaatctg ctcccagcgt ttttgctgat tctgatgctg ataaggcaat ggcatctgta
     2821 atgatcgcgc gtgtatatat ctttctttct tccattgtag ctttgtacat aatggagtct
     2881 tcagcatatt gagcaatctt tgccattgtt gctgctgcat caacag
//
LOCUS       DAOSOX010000255         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_33922_length_2926_cov_4.876001, whole genome shotgun sequence.
ACCESSION   DAOSOX010000255 DAOSOX010000000
VERSION     DAOSOX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33922_length_2926_cov_4.876001"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLX22_11995"
     CDS             <1..226
                     /locus_tag="PLX22_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20660.1"
                     /translation="YGSPDGFYIDIITRLGEAFCYEDITWDSKEVDGHMVRIAKAETL
                     YKMKNDTVRAIDKADALYLHELMKKKKNDL"
     gene            216..446
                     /locus_tag="PLX22_12000"
     CDS             216..446
                     /locus_tag="PLX22_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20661.1"
                     /translation="MIYKFKTFDEAQRALWNFNPDAKYYEQVRQLFLLSSKLYPLHCR
                     RGIFLFKTIEEANEFRKQEELTRALMNITNSD"
     gene            526..1059
                     /locus_tag="PLX22_12005"
     CDS             526..1059
                     /locus_tag="PLX22_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20662.1"
                     /translation="MVKRLVFMVWCVIVVTNSYAVDINTIFNDEQIAFIKSATDMVQA
                     SLGYDDFIEVVYVTFWSSQPVDKKKVAAFNAYIAQQYRVQPDAILYVYERLLRSLYMI
                     EYMSIDAREKRQWKQYYYYTENLLPDTKRFLDLFKNAIVAVDSLYAAQADNKEKAIKN
                     FAIDLVKYNQNLYGGGF"
     gene            complement(1174..1956)
                     /locus_tag="PLX22_12010"
     CDS             complement(1174..1956)
                     /locus_tag="PLX22_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT20663.1"
                     /translation="MKTKKVKLIQSIAKAHDILSFFVHDKRTIGISEFAKKLSLPKTT
                     IHSIVKTLCYLGYLEKDVTTGKYMLGPYVFQLGMKYATNLDIVSIGRVWAERLCFQYR
                     QPVNIGMLVGGKVVVVLRVEPENRFMTYPQTGSVIPTHTTCIGKLLCAYTDNYRLHEL
                     LESYEFQALTKNSISSKENFINELKMVRSMGISFDNQESIAGLAGIGGPIFNYTGQII
                     AAFAITGDADFIEKHRNDIITTVKDTSLQLSLQLGYIPAHHQ"
     gene            2411..>2926
                     /locus_tag="PLX22_12015"
     CDS             2411..>2926
                     /locus_tag="PLX22_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT20664.1"
                     /translation="MDIIKSFENLNPYEYVASIKKATGKSVLAYMCSYTPEEIIYAAG
                     IHPFRIFGMVDSIAKADAYLQVYSCSLVRGALEDALSGKLSFVDGAVFPHTCDSIQRL
                     SDVWRLNAGMKHHFDVVWAVKLNTNSAKKYMYDVLAKFVKELQDAFGITITNEKLEKA
                     ISTYNEIRSLLK"
BASE COUNT          930 a          459 c          592 g          945 t
ORIGIN      
        1 gtacggttct ccggatggat tttatattga tattattacc agattaggag aggcattttg
       61 ttatgaagat attacatggg atagtaaaga agtagatggg catatggtga ggattgcaaa
      121 ggctgagaca ttgtataaaa tgaaaaatga tactgtacgt gcaattgata aagcagatgc
      181 actatattta catgagttaa tgaaaaagaa gaaaaatgat ctataaattt aaaacatttg
      241 atgaagcaca acgagcatta tggaatttta atcctgatgc aaagtattat gaacaggtgc
      301 gacaattgtt tctattgtct tcaaagctat atcctctgca ctgtagacgt ggcatatttt
      361 tgtttaaaac aatagaagaa gccaatgaat ttagaaaaca ggaagagcta acaagagctt
      421 tgatgaatat aaccaatagt gattgacaca aagtagtgat ggtatgatac cgtttgttgt
      481 ccagtgattg tgttgagaca taaattaaaa aggtgataat atagtatggt aaaaagattg
      541 gttttcatgg tatggtgtgt gatagttgtt accaatagtt atgctgtgga catcaatacc
      601 attttcaatg atgagcagat tgcatttata aaatcagcaa ctgatatggt acaggcttca
      661 ttgggatatg atgattttat tgaagttgtt tatgttacct tctggagcag ccagccggtg
      721 gataagaaaa aggttgcagc atttaatgca tatattgccc agcagtatag agtacaacct
      781 gatgccatac tctatgttta tgagcgattg cttcgttcat tgtatatgat tgaatatatg
      841 tcgatagacg cccgggaaaa aagacagtgg aaacaatact actattatac tgaaaatttg
      901 ctgcctgata caaaacgttt tcttgatttg tttaaaaatg ccattgttgc tgttgattcg
      961 ctgtatgctg cacaggctga taataaagaa aaggctatta aaaattttgc tattgactta
     1021 gttaagtata atcaaaacct gtatggcgga ggattttaag aaaaagcccc ttttttcaag
     1081 gggcaaagct tgtctgttat agaatttatt agcactctcc attaatgagt gctaatcata
     1141 atatactact gtctcctgtt ttcgtcaagt attttattga tgatgggctg ggatataacc
     1201 caattgtaat gagagctgta atgatgtatc ttttactgtt gtgattatat cattcctatg
     1261 tttttcaata aaatctgcat cgccggttat agcaaatgca gcaataattt ggccggtata
     1321 gttgaatatt ggcccaccaa tacctgcaag ccctgcaata gattcttgat tatcaaagct
     1381 gatgcccata gaacgaacca ttttcagttc atttataaaa ttttccttgc tgcttatgga
     1441 attctttgtt aatgcctgga attcatagct ttccagtaat tcgtgtaacc gatagttatc
     1501 ggtatatgca cataaaagct tccctatgca ggttgtatga gtaggaatta ccgaacctgt
     1561 ttgagggtat gtcataaatc tattttctgg ttcaacacga agtactacta caaccttgcc
     1621 gccaactaac atgccaatgt ttactggttg ccgatattgg aagcaaagac gttcagccca
     1681 cactctgcct atactgacaa tgtctaaatt ggttgcgtat ttcattccaa gctggaatac
     1741 atacggaccc agcatgtact tgccggttgt tacatctttt tctaaatatc ctaaataaca
     1801 aagtgttttt acaatactgt gtatggttgt ttttggcaaa gaaagctttt tagcaaattc
     1861 actgatgcca atggttcttt tatcatggac aaaaaatgat aaaatatcat gtgcctttgc
     1921 aattgactgg ataagtttaa cttttttagt tttcatgttt gtcatactat ataataaaaa
     1981 aatgttgaca gtatacatat acaaatttat attgaaatat gtatcgtaca atgtacgaca
     2041 ttatcgtaca gtgtcaacca tttttttata ggacagtata ctgtatttgg gagtgcagtt
     2101 gcattacaga ttgttataaa aaaattgcta taacgaattg tacgtgttcg atagttacca
     2161 ttaaatgatg tgtaagtgca ccaataagat ggcaatagtt ggcaacagtt attctgaaat
     2221 gcagtacgtg cccccggaaa gacggcagag aaaagtgtca tcctgaactg tgattcagga
     2281 tctataggat ctcagctgga tatctggatt tcgcatcaag tccggaatga cggcagaggt
     2341 aaagtatgca gatacgcatt gtgatgcgcc cgaaaattgc gaataattct attttctcag
     2401 gaggtttttt gtggatatca taaaaagttt tgaaaaccta aatccctatg agtatgttgc
     2461 ttctattaaa aaagcaacag gcaaatcagt gctcgcatat atgtgttcat atacacccga
     2521 agagatcatc tatgcagcgg gtattcatcc attcagaatt ttcggcatgg ttgattctat
     2581 tgcaaaggct gatgcatatc tacaggtgta tagctgttca ctggtgcgtg gtgcgcttga
     2641 agatgcactg agcggaaaat tatcatttgt tgacggggcg gtatttcccc atacctgcga
     2701 ttctatacag cgcctgtctg atgtctggcg attaaatgcc ggcatgaaac atcattttga
     2761 tgttgtatgg gcagtgaaat taaatactaa tagcgctaaa aaatatatgt atgatgtgct
     2821 ggcaaagttt gtcaaagaat tgcaagatgc atttggcata acaataacca atgaaaagct
     2881 tgaaaaagct atcagtacct ataatgaaat ccgttcgctt ttaaaa
//
LOCUS       DAOSOX010000256         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_34030_length_2919_cov_7.486034, whole genome shotgun sequence.
ACCESSION   DAOSOX010000256 DAOSOX010000000
VERSION     DAOSOX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34030_length_2919_cov_7.486034"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLX22_12020"
     CDS             <1..185
                     /locus_tag="PLX22_12020"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOT20665.1"
                     /translation="EEARKKLVYAIQGKKPFRRFKDALQAFPDIEKQWYSFEEKKMKE
                     YIIKWLHEYDIAIENS"
     gene            1037..1540
                     /gene="cas4"
                     /locus_tag="PLX22_12025"
     CDS             1037..1540
                     /gene="cas4"
                     /locus_tag="PLX22_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOT20666.1"
                     /translation="MYNNITPVITGTQIAYYIVCHRKLWLFNKNISLEHTSELVEIGN
                     LIHEASYTRKRKEINLEGIKIDLLEAKRGIIHEVKKSKALEESHAWQLKYYIYYCKQI
                     GIEVEGIIDYPKLRRREKIILNMEDEEKIKNILNEIYVIMSDDKLPAVIKKPYCKKCS
                     YYEFCYC"
     gene            1549..2526
                     /gene="cas1b"
                     /locus_tag="PLX22_12030"
     CDS             1549..2526
                     /gene="cas1b"
                     /locus_tag="PLX22_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOT20667.1"
                     /translation="MKDYYIFKSGRISRKDNSIVFETNDSNIFIPINDIDSLCFFGEI
                     DINTKAMNFLAQQQVVVHFFNYYGWYTGSFYPREFLLSGDVLVAQVKAYLDMHSRTYI
                     ARQFVQGAIHGFITNIKRYPDVGDAAVDTLQQYLSATEEQSNVLSLMGIEGNAHELYY
                     STFPVIIKQTIEFEKRVKHPPNNMMNAMISFCNSLVYTHVIKELYKTQLNPTVSYLHE
                     PSNRRFSLALDVAEIFKPIYADRVIFDLLNNNMITQKHFDKDLNYCYLKEDGRKIVVK
                     AFDDKINTTVMHKKLKRNVSYQKMIRLELYKLIKHLLNEEQYQSLRIWW"
     gene            2729..>2919
                     /gene="cas2"
                     /locus_tag="PLX22_12035"
     CDS             2729..>2919
                     /gene="cas2"
                     /locus_tag="PLX22_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOT20668.1"
                     /translation="MYIILVYDISTVTSSGRKRLPKVMKKCRQYLRHTQKSVFEGEIT
                     EAKLYALKQEIAKLIDDKE"
BASE COUNT         1096 a          415 c          536 g          872 t
ORIGIN      
        1 atgaagaggc gcgtaaaaaa ttagtatatg caatacaggg taaaaaaccc tttagaagat
       61 ttaaagatgc actacaggcg tttccagata ttgaaaaaca gtggtatagt tttgaagaaa
      121 aaaaaatgaa agagtatatc ataaagtggt tacatgaata tgatattgca attgaaaact
      181 catagttatt ttaataagta ctgcatatat gcacaatttt tttgcacata tacagtacca
      241 tataaattac gttattttat tggcaactac atgaatctat caagggtaaa tatatgataa
      301 acaaagaact taaagaaaga atggaagcac tgctaaacag cagagaagat tagcttgctg
      361 atgaacttaa tcaagagctg gaaaaaattg tcaatgaaat gaataaaaga cattagataa
      421 ttttgaagga ttttctcctg cagatatgca ctatatgctt tatactcctt ttcataaaca
      481 atgctctatt ggtataaata ccaatatttc caaatatttt tattacagta ttcaccttta
      541 ttgaagttgg cagtacatat tatggaaaaa attaaagcag aaggtcatat gctgttaaca
      601 tctaaagata atcttaaagt aagctttatt aaataattgt ataatcttgg aatttattat
      661 ggtcatgata gagacagttt tgaaaaccgt ccatgccagg acaacctgga tttttgtggt
      721 cactatggat tcttaaaaaa tatggttcaa aacgcgaacc agccagtttt tatgcgcaaa
      781 aatatttaac agcgtttcca atatttatta accagcagaa caatataaat tttgtggatg
      841 cctgttatac agtttgtttt gtggctggta ataataatga caatagttca ttattttaaa
      901 aaccctgaaa ttgttagcac atcacttttg aatacatttt tgaaatagta atggttgatg
      961 ctgtataaag tttatgcttc attatcaaat atatttattg cattaatagt gccatgtatt
     1021 ttataattga agggatatgt ataataatat aacacctgta atcactggta ctcaaatagc
     1081 ttattatatc gtctgccata gaaaactatg gttattcaac aagaatattt cgttggaaca
     1141 tacaagcgaa cttgttgaga ttggtaacct aattcatgaa gcatcatata ctagaaaaag
     1201 aaaagaaatt aatttagaag gcattaaaat tgatttacta gaagcaaaaa gaggaattat
     1261 tcatgaggta aaaaagtcaa aagcattaga agaatcacat gcatggcaat tgaagtatta
     1321 tatttattat tgcaagcaaa taggtattga agtggaaggg atcattgatt atcctaaatt
     1381 gcgaagaaga gaaaaaatta tccttaatat ggaagatgaa gaaaaaataa aaaatattct
     1441 taatgaaatt tatgtaataa tgagtgatga taaactccca gcagtcatta aaaaaccgta
     1501 ctgcaaaaaa tgtagctact atgaattttg ctattgttag gtattaccat gaaggactac
     1561 tacatattta aaagcgggcg cattagccgc aaggataact ccatagtgtt tgagactaat
     1621 gacagtaata tctttatacc cattaatgat attgatagcc tttgcttttt tggcgagatt
     1681 gatataaata caaaagcaat gaatttttta gcacaacagc aggttgttgt acactttttt
     1741 aactactatg ggtggtatac cggcagtttt tatccgcgcg aatttttgtt atcgggtgat
     1801 gtgctggtag ctcaggtaaa ggcatacctt gacatgcaca gcaggacata tattgcacgg
     1861 cagtttgtgc agggtgcaat tcacggcttt ataaccaata taaaacggta tcctgatgtt
     1921 ggcgatgcgg cggtggatac attacaacag tacctaagcg caactgaaga gcaatccaat
     1981 gtactatcgc tcatgggtat tgaaggaaac gcgcatgaat tgtattatag cacattccct
     2041 gttattataa agcaaaccat agaatttgaa aaaagggtaa aacatccgcc caacaatatg
     2101 atgaatgcaa tgatatcgtt ttgcaacagc cttgtttata cgcatgtaat aaaagaactg
     2161 tataaaacac agcttaaccc aacagtaagc tatttgcatg agccgtcaaa caggcgcttt
     2221 tcgctggcgc ttgatgtggc agaaattttt aaaccaatat acgcagaccg tgttattttt
     2281 gacctgttaa acaataacat gataacacaa aaacattttg acaaagattt aaattactgt
     2341 tatttaaaag aggatgggcg caagattgtg gtaaaagcct ttgacgataa gattaataca
     2401 acggttatgc ataaaaagtt aaaacgcaat gtaagctacc aaaagatgat acgccttgaa
     2461 ttgtataagc ttattaaaca tctgttaaac gaagagcagt atcaaagttt gcggatatgg
     2521 tggtaagata gttattatac atagggtaat caacatgcat ggaataaata aaagagatta
     2581 tgaaatgacc agaactgcaa taaaagctgc tttcattatt aaaaatgtat ttaacaaagg
     2641 tattactata tgaatactat atttaaaaaa attgccgggg gcgatagtta ctggacatta
     2701 aaacattgat ggataacagg gcaatactat gtatataatc cttgtatacg acatctcaac
     2761 cgtaacatcg agtggacgga agcgattgcc aaaagtaatg aaaaaatgcc gacagtacct
     2821 gaggcatacg caaaagtcgg tatttgaagg cgagataacc gaagcaaagc tgtatgcatt
     2881 aaaacaggag attgcaaagc ttatagatga caaagagga
//
LOCUS       DAOSOX010000257         2918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_34049_length_2918_cov_6.752008, whole genome shotgun sequence.
ACCESSION   DAOSOX010000257 DAOSOX010000000
VERSION     DAOSOX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34049_length_2918_cov_6.752008"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLX22_12040"
     CDS             <1..976
                     /locus_tag="PLX22_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20669.1"
                     /translation="AGRAQKAIFKARDLTTQLLAFSKGGAPVKTSASLVNIIKDTAEF
                     VLSGSAIQCRFFISDDLHNALIDPAQISQVIHNIILNARQAIGHQGIITITANNVVVN
                     DPSLPISGEFIEITIHNTGETIPPDIMPYIFDPFFTTRKDGHGLGLSIVYSIIKRHDG
                     HITVESSNEGTCFTLYLPKAESIYKKDQNQPDTGYLTSQMSILIMDDDETVTESLKEM
                     LSLQGCHVISVRDGDEAVKAALQAVKQNKPFDIAILDLTIKGGKGGVDSVRELKAILP
                     CCKFIASTGYANEPILANYKEYGFDAILPKPYTFNELIKVLSSLGNNQ"
     gene            complement(981..1652)
                     /locus_tag="PLX22_12045"
     CDS             complement(981..1652)
                     /locus_tag="PLX22_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT20670.1"
                     /translation="MKKHINDMCGRFAQIEPIDSIAQRLGIDEVLIDTIAPRYNISPG
                     EDVVALVHTKKKSLVLLRWGFIPYWVRDISRVKPLINARGETVAVKPGFKKAFFNNRC
                     IILASGFYEWKKEGSKKIPYYITIDDFFGFGGIFDTAIIGGKELRTCAILTTEANDAM
                     HVIHSRMPLIIRQKEFELWIKSETPLDILKELIKPFSGNCIMHPVTTKVNNPAYNDPD
                     CINPV"
     gene            complement(1633..>2918)
                     /locus_tag="PLX22_12050"
     CDS             complement(1633..>2918)
                     /locus_tag="PLX22_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOT20671.1"
                     /translation="ISNQDSSKTDTKPFEQFQTFLAKTYPKLHKTLKKEVVNGYALLY
                     TWKGSDPSLKPILLMAHQDVVPVDVVTLDQWTHPAFSGAIADGYIWGRGALDVKNALI
                     SIMESVEYLVNKRFVPKRTIYVAFGNDEEVGGEGAKSIAKLLKDRNVQLEYVLDEGGA
                     IMEGALPGISNPIAMVGIAEKGYLTLKLTVKGTGGHSSMPPHHTALGVLARALVAIED
                     NPFPKEIKGPAQQMFEYVTPHMSFPLKVMFANMWCFKPVLKWQFEKAESSNAIMRTTV
                     ALTMAGASEKDNILPTQAWAMINCRILPGETMESTTAYIKKVISNEAVLIESTPWSND
                     PSPVSDINAPSFHSIATITRQLDNSIIVAPYLVLGATDSRHYNALTNQIYRFTPVKMN
                     NEDLKRTHGINERISISDMGKSVKFFIALIEKTHQ"
BASE COUNT          869 a          616 c          536 g          897 t
ORIGIN      
        1 agccggacga gcacaaaaag caatatttaa agcacgtgat ttaacaacac agttactggc
       61 tttttcaaaa ggtggggctc ctgttaagac atcagcatca cttgtcaata ttatcaaaga
      121 tacagccgaa ttcgtgctca gtggttccgc aatacaatgc cgtttcttta tatcagatga
      181 tttacataat gcattgattg atcctgcaca gatctcacag gttatccata atatcatcct
      241 caatgctcgt caggcaattg gacatcaggg cataattacc attacggcta acaatgtagt
      301 ggtaaacgac ccttcattgc caatcagcgg tgaattcatt gagataacga tacacaatac
      361 cggtgaaaca ataccgcccg atattatgcc ctatatcttt gatccatttt ttacgacacg
      421 taaagacgga catgggctgg gattatccat tgtttattcc attattaaaa gacatgatgg
      481 gcatatcacg gtagaatcgt caaatgaagg aacatgtttt acactctatt taccaaaagc
      541 tgaatcaata tataagaaag atcaaaatca acctgatacc ggctatttaa catcgcaaat
      601 gtctattttg ataatggatg atgatgaaac ggtaaccgag tcacttaagg aaatgctttc
      661 ccttcagggt tgccatgtaa taagcgtcag ggatggtgat gaagcagtaa aagcagcatt
      721 acaagctgta aagcaaaaca aaccttttga tattgctatt cttgatctaa ccattaaggg
      781 gggtaaaggt ggtgtcgata gtgtccgtga attaaaggca atattaccat gctgtaagtt
      841 tatcgcatca accggatatg ctaacgaacc aatattagct aactataaag aatatggctt
      901 tgatgcaata ctgccaaaac cgtatacctt caatgaactc atcaaggtac tgtcttcact
      961 tggcaataac caataatctg ttatacagga tttatgcaat caggatcatt atacgcaggg
     1021 ttattaacct ttgttgtaac cggatgcatg atacaattac cactaaaggg ttttatcaat
     1081 tcttttaata tgtccagtgg cgtttcagat tttatccaga gttcaaattc tttctgccgt
     1141 attataagag gcatcctgct atggatcaca tgcattgcat catttgcttc agtggtaagt
     1201 atagcacatg tacgtagctc cttgcctcca atgatagcag tatcaaatat ccctccaaaa
     1261 ccaaaaaaat catctatcgt tatatagtat ggtatttttt tactgccttc ctttttccac
     1321 tcatagaagc cgcttgccag tataatgcac ctgttattga aaaacgcctt tttaaaacct
     1381 ggctttacag caactgtttc accgcgtgca ttaatgagtg gttttactct ggatatatcc
     1441 cttacccaat aggggatgaa cccccatcgc aatagcacta acgatttttt cttagtatgc
     1501 accagcgcta caacatcctc acctgggctg atgttgtagc gcggtgcgat agtatctatt
     1561 aatacttcat caattcctaa acgctgtgca atactgtcaa taggctcaat ctgtgcaaat
     1621 cgcccgcaca tatcattgat gtgttttttc aattagtgca ataaagaatt taacgctttt
     1681 acccatatca gaaatactta tacgctcatt gatgccatgg gtgcgtttta aatcttcatt
     1741 attcatcttt acaggtgtaa accggtaaat ctgatttgtt aaagcattat aatgtcttga
     1801 atcagttgcc cccaatacta aataaggtgc tacaatgata gaattatcta actgtcgggt
     1861 gatagttgct atggaatgaa atgatggcgc attgatatcc gataccggtg aaggatcatt
     1921 tgaccatggt gttgactcaa taagtactgc ttcattactg ataaccttct ttatgtatgc
     1981 agtggtactt tccattgttt caccgggcag gattcggcaa tttatcattg cccatgcctg
     2041 tgttggtaat atgttatcct tttcgcttgc acctgccatg gtaagtgcaa cagtggtacg
     2101 cattattgca ttcgatgatt cggccttttc aaactgccac tttaatactg gcttaaaaca
     2161 ccacatattt gcaaacataa ctttcaaagg aaaactcata tggggagtaa catactcaaa
     2221 catctgctgt gccgggcctt taatttcttt tgggaaggga ttgtcttcaa tagcaaccaa
     2281 tgctctggca agcaccccca atgcagtatg gtgaggtggc atggatgagt gcccaccggt
     2341 accttttacc gtcaacttca atgttaaata tcctttttcg gcaatcccta ccatggctat
     2401 aggatttgat atccccggta gcgccccctc catgatagca ccgccttcat ccagaacata
     2461 ttccagttga acattacgat cttttaaaag tttagcaata gatttagcac cctcaccacc
     2521 aacctcttca tcattaccaa atgctacata gattgtccgt ttgggaacaa aacgtttatt
     2581 gaccagatat tctacagact ccatgatgga aattaatgca tttttaacat caagtgcacc
     2641 tcttccccag atataaccat ccgctattgc tcctgaaaaa gctggatggg tccactgatc
     2701 aagcgttaca acatctacag ggacaacatc ttgatgagcc attaagagta ttggcttcaa
     2761 tgaggggtca gagcccttcc atgtgtagag cagtgcatat ccattaacaa cctctttttt
     2821 taaggtttta tgcaactttg gatatgtttt agccaaaaaa gtttgaaact gctcaaatgg
     2881 tttagtatca gtttttgagc tatcctgatt ggaaattg
//
LOCUS       DAOSOX010000258         2884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_34576_length_2884_cov_10.039943, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000258 DAOSOX010000000
VERSION     DAOSOX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34576_length_2884_cov_10.039943"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..2061
                     /locus_tag="PLX22_12055"
     CDS             19..2061
                     /locus_tag="PLX22_12055"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT20672.1"
                     /translation="MLPLELKIKSRDIDTLIGAIQSNSINANIETIKRAYETANEAHK
                     NQQRLSGEPFIVHPMEVSIILANLKMDETTIIAALLHDTVEDTPIDINKVKELFGDEV
                     ALLVDGVTKISSLKKHTKQKEQAETLRKMLLATIQDPRVIIIKLADKLHNMRTIMFQP
                     ENKQKYIAQEVLDIYAPLAGRLGMSQIRSELEDLAFKVLYPDDYQAILEHIKARRTEL
                     ESYIDTVRNTLLHRLKKLNIEAQVQGRIKHLYSIFKKLQQGRTFDDIFDLRAVRVITN
                     EVKDCYGILGIVHTIWTPIPSRFKDYIAVPKSNMYQSLHTTVIGPDGHPLEIQIRTWD
                     MHATAEMGIAAHWMYKEGIKSFTPKYKSITILNNLHEWENEVRDTREFIKELKMDLYE
                     DEIFVFTPKGKIIKLAKGSTPVDFAYLIHTEIGNHCAGAKVNNQLVPLKTELKSGDII
                     EILTSKKGHPSPSWLKFVKSSNARYKIRNWLRKNVEIEDEHLEKEDKQESDKDKKGKI
                     ANVSIPEKELVKIKSYTNKKKVGITIEGTSNVMIKLSQCCQPIPGDDVIGFITRGRGI
                     TVHKRNCPSLLRLQNESERFVDIKWEQSESTYPVKLAVEAMDRPNLLKDVADEISLTK
                     TNIIKAEASLVAKDQAMLKFVLEVKGINHLNDIIKRLKQIRNVTRVYKLNEKVVLK"
     gene            2064..2672
                     /locus_tag="PLX22_12060"
     CDS             2064..2672
                     /locus_tag="PLX22_12060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT20673.1"
                     /translation="MINIVLGSSSPRRKSLLNNLFQDFTIIHPDIVETPHHGEAPLEF
                     VNRIASHKLMDIYHKVTYEHALIITCDTIVTIDNEILGKPYDEEHAYFMLLKLSGKTH
                     QVISSISMAYKNGHHNQKHNDYEITNVNFKLLPSSDIKNYLSKINFLDKAGSYAAQEY
                     GDDIIQKIDGSLTNVIGFPLRCFFRMLIDMNILHNFLYYFKF"
BASE COUNT         1078 a          487 c          515 g          804 t
ORIGIN      
        1 aatggttacc tgaactatat gttgccctta gagttaaaaa taaaaagtcg cgatattgac
       61 acattaatag gtgctatcca gtcaaatagc atcaatgcaa atattgaaac tataaaaaga
      121 gcttacgaaa cagctaatga agctcataaa aatcagcaac gtctttcagg cgagcctttc
      181 attgttcatc ccatggaagt atcaattatt ttagctaatt taaaaatgga tgagacaacc
      241 atcattgctg ctttgctgca tgacactgtt gaagatactc ctatagatat aaataaagta
      301 aaagaactat ttggagatga ggttgcacta cttgtagacg gtgtaacaaa aatttcatcg
      361 ttaaaaaaac acaccaagca aaaagaacaa gcggaaacat tacggaaaat gcttttagct
      421 actattcagg acccccgcgt cattattatc aaacttgctg ataaactgca taatatgcgc
      481 acaataatgt ttcagcccga gaataagcaa aaatatatag ctcaggaagt acttgatatc
      541 tatgctccat tagccggacg attggggatg tcgcaaatac gttcagaatt ggaagattta
      601 gcattcaaag tattatatcc tgacgactat caagccatac ttgaacacat caaagcacgc
      661 agaactgagc tggaatcgta tattgacact gtacgcaata ccttactaca tcgattaaaa
      721 aagcttaaca ttgaagctca ggtccaggga aggataaaac acctttactc aattttcaaa
      781 aaacttcagc aggggagaac atttgacgat atatttgacc tcagagcagt gagagtaatt
      841 actaacgagg ttaaagattg ctatggaatt ttaggtattg tccatactat atggacacct
      901 atcccatcac gatttaaaga ttatattgct gtccctaaat ccaatatgta ccaatcatta
      961 cataccacgg tcattggtcc tgatggccac ccgctggaga tacagatacg cacatgggat
     1021 atgcacgcca cggctgagat gggtattgca gcgcattgga tgtataaaga agggattaaa
     1081 tcatttacac caaaatataa aagtataact atccttaaca accttcacga atgggaaaat
     1141 gaggtacggg atacccgtga atttattaaa gaattaaaga tggacctcta tgaagatgaa
     1201 atttttgtat ttaccccaaa aggtaaaatt attaaattgg caaaaggttc aacgccggta
     1261 gattttgcct atttaataca tacagaaatc gggaatcatt gtgcaggtgc aaaagttaat
     1321 aatcaattag ttcccttaaa aactgaatta aaaagcggtg atattattga aatattaacc
     1381 agtaaaaagg ggcacccatc accttcatgg cttaaatttg ttaagtcatc caatgcacgg
     1441 tataaaataa gaaattggct gcgcaaaaat gttgaaatag aagatgagca ccttgaaaag
     1501 gaagataagc aagaaagtga taaagataaa aaaggaaaaa ttgccaatgt tagcatccct
     1561 gaaaaagagc ttgtcaaaat taaaagctat acaaacaaaa aaaaggttgg catcaccatt
     1621 gagggtacaa gcaatgtgat gattaagctt tcacaatgtt gtcagccaat cccgggggat
     1681 gatgttattg gatttattac ccggggcaga ggaataaccg ttcacaaaag gaactgccct
     1741 tctctgctgc gattgcagaa tgaaagtgag cgttttgttg atataaaatg ggaacaatcg
     1801 gaaagtacct atcccgtaaa actggcagtg gaagcaatgg atagaccaaa tcttctgaaa
     1861 gatgttgccg atgaaatttc actgacaaaa acaaatatta taaaagctga agcctcattg
     1921 gtggcaaaag atcaggcaat gctaaaattt gtacttgaag ttaagggcat taaccatctg
     1981 aatgatatta tcaagaggct aaaacagata agaaatgtga cccgcgtcta taaacttaat
     2041 gaaaaagtag tattgaaata acagtgataa acattgtact tggttcctca tcccctagaa
     2101 gaaaatcttt actaaataat ttatttcagg attttactat tatccatcct gacatagtgg
     2161 aaacaccaca ccatggcgaa gcaccgttag aatttgttaa ccgcattgct tctcataaat
     2221 taatggacat atatcataaa gtaacatacg aacatgcatt aattattaca tgcgatacta
     2281 tcgtaaccat cgacaatgaa attttaggga aaccatatga tgaggaacat gcatacttca
     2341 tgctgttaaa attatcaggc aaaacacatc aggttatttc atctattagc atggcataca
     2401 aaaatggaca tcataaccaa aaacacaatg attatgaaat taccaatgtg aatttcaaat
     2461 tgcttccatc atctgatata aaaaactatc tatcaaaaat aaattttttg gataaggccg
     2521 gaagctatgc agcccaggaa tatggggatg atattatcca gaaaattgac ggttcgttaa
     2581 caaatgtaat aggatttcca ctgcgttgtt ttttcaggat gctgattgac atgaatatat
     2641 tacacaactt tttatattat ttcaaatttt aagattgaca taaaataaaa ttagtttatt
     2701 atttattcac agctattata gcggtttaaa atttacttgt ataaaaggtt atacatatga
     2761 aaaagagaaa gcaatatatt ataaacaaaa aatttcagct caagactaca ttttctgtca
     2821 taggcatcat atttgttatt gtcgctatca taattgctgc aataggagta aatgcagcat
     2881 ataa
//
LOCUS       DAOSOX010000259         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_34822_length_2869_cov_10.583156, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000259 DAOSOX010000000
VERSION     DAOSOX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34822_length_2869_cov_10.583156"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLX22_12065"
     CDS             <1..533
                     /locus_tag="PLX22_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20674.1"
                     /translation="LQWAQDVGFSKADATRIANACNNVDRIGSGLSFLPILGNQGYHF
                     NTNAGGIDSRILYAVGHLNKAIGYEKMANKFRNDDGGIMGWLKNTIADAFHNAALDEL
                     GEGIHPLQDVYAHTDDLTKDVNFLGISLFKYHDSPQLNGRPNEADDPKGGEKRVNVTE
                     WATKAFLEFFKEKVEE"
     gene            537..1223
                     /locus_tag="PLX22_12070"
     CDS             537..1223
                     /locus_tag="PLX22_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20675.1"
                     /translation="MKQIIYLIMIVSISTLVNCSSMMKKEVEVRPITIKVVDDKTNEP
                     LAGIIVYRAVEAAYGENRMLFGLLPKPEPKIHDRVILTEEGETDNNGIIVFQTKKVLI
                     KKKEILYTENVFINLEPNLDEISKDTVVLALGKDVNKDDKIINLYKYTLRTFFYPDRK
                     EEFLTNPNNTYKGVYIFSPEWEISAADFEYDKQQNLKNRYFYTISISKGILKEEENIV
                     IRLQRKKNTQ"
     gene            1270..1560
                     /locus_tag="PLX22_12075"
                     /pseudo
     CDS             1270..1560
                     /locus_tag="PLX22_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2059..2730
                     /locus_tag="PLX22_12080"
     CDS             2059..2730
                     /locus_tag="PLX22_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20676.1"
                     /translation="MKKAILTLLIILFLFMSAFLNGDQMKIQKLPLKESPIIRIFNTG
                     IEIPLGYFALVKRNNKICAIRFLETGKHPKKDKNYAIYEYYFQCDGRGDFLNNTSKHG
                     TGKATYKSFCIIGRLCFNTGNMNLACGEINIKWTGGRYLEYGDGLNRNEKISDVIKAG
                     VQIAPTKWTDIKAVNVLEKGLKWYKYGTLPEYPEYQRFKVVKNIDWPPGVKIEFYEIP
                     AEDLW"
BASE COUNT         1099 a          366 c          651 g          753 t
ORIGIN      
        1 cattgcaatg ggctcaggat gtaggattta gtaaagcaga tgcaaccaga attgcaaacg
       61 catgcaataa tgttgataga atagggagcg ggttgagttt cttgccaatt ttgggtaacc
      121 agggatatca ttttaatact aatgcgggag gaatagattc aaggatactg tatgcagttg
      181 ggcatttgaa taaagctata gggtatgaga agatggcgaa taaattcagg aatgatgatg
      241 gagggataat gggatggttg aagaatacga tagcggatgc atttcataat gcagcgcttg
      301 atgaattagg ggaaggaata catccattac aggatgtata tgcacataca gatgacttaa
      361 caaaagatgt gaatttttta ggtatatcat tgtttaagta ccatgattca ccgcaattga
      421 atgggagacc taacgaagct gatgatccga aaggaggaga aaaaagagta aatgttacag
      481 agtgggcaac aaaggcattt ttagagtttt tcaaagaaaa ggtggaggaa taggaaatga
      541 aacaaattat ctatttgatt atgatagtga gtataagtac attagtaaat tgtagcagta
      601 tgatgaaaaa ggaagtagaa gtaagaccaa taacaattaa agtggttgat gacaaaacca
      661 atgagccatt ggcagggata attgtatata gggcagttga agcagcgtat ggtgagaaca
      721 gaatgttgtt tggattattg ccgaagccag aaccaaaaat acatgatagg gtaatactaa
      781 cggaagaggg ggaaacagat aacaatggta tcattgtatt ccaaacaaaa aaggttctta
      841 ttaagaaaaa ggaaattttg tataccgaaa atgtttttat aaaccttgag ccaaacttag
      901 atgaaatttc taaagatacg gttgttttag ctttagggaa agatgttaat aaagatgata
      961 aaataataaa tttatataaa tatacactaa ggacgttttt ttatcctgat agaaaagaag
     1021 agtttttaac aaatccaaat aatacttata aaggtgtata tatattcagt ccagagtggg
     1081 agatatcagc agcagatttt gagtatgata aacaacaaaa cttaaagaat agatattttt
     1141 atacgataag cattagtaaa ggaatattaa aggaagagga gaatatcgtt ataaggttac
     1201 agcgtaagaa aaatacacaa tagttttaat ttataaaatg ataaagtaat atcagattca
     1261 tatacaacgc cgtacaaatt caccagcaaa gaattggata cggaaacggg attgtattac
     1321 tttggtgcac ggtactacga tgcacgggta agtagatggg tgagtgcgga cccggctctt
     1381 gcggaaggga agtattttcc gaaaccaaac gactatgaca cggaacatga tttttactgg
     1441 tacgtacagc aggatggcag caaaaagtta gcgggattgg gtggtgtatt taatgcagta
     1501 aatatggata tatatcatta cgcgggaaat aatccggtga agctgattga tccagatggg
     1561 agcatggcct tcttctggca ttttattttg acattttcag aagcgcaaaa agctggtgta
     1621 ggattttggg atagcttgaa aatggcatgg catgcaatgg cgtgggactt tagaggaaca
     1681 caaagcacaa cagacgaaaa aatggttaat tcacatgcta tgattagaca aaagataaca
     1741 aatgaagctg gtgaaacagt aagaccacag cagacaatag aagaagcagc aaaaggcaca
     1801 gaaaaattca ttcaagagaa aataaaggaa ggtacaatta gaagtacagg agaagcaaca
     1861 catgctgcag tcgataaacc tgcagaaagt cataaagaac aagtatggac aggagaatta
     1921 acatgggatc atgtaaaaaa agatatattc cccagtaaag aatccagaga agaagcacgc
     1981 aagatcatac gggaggtatt gatagatgca attaaaataa atcctgatct tatagataaa
     2041 aaatagggta aatataagat gaaaaaagca atattaaccc tcttaattat tcttttttta
     2101 ttcatgtcag cttttctgaa tggtgatcaa atgaagatac aaaaattacc actaaaagaa
     2161 agtcctataa tccggatatt taatacaggt attgaaatac cactaggata ttttgctctt
     2221 gttaagagga acaataaaat atgtgcaatc aggtttttgg aaactggcaa acaccctaaa
     2281 aaagataaga attatgcaat ttacgaatat tatttccaat gcgacggtag aggggatttc
     2341 ttaaacaata cgtcaaaaca tgggacaggc aaagcaacat ataagtcatt ttgcataata
     2401 ggtcgcttat gttttaatac aggaaatatg aatttggcgt gtggagaaat aaatattaag
     2461 tggactggtg gacgttattt agaatatgga gacggattaa atagaaatga gaaaattagt
     2521 gatgtaatca aggccggtgt acaaatcgca cctaccaaat ggactgatat taaagccgta
     2581 aatgtcttag aaaaagggct taaatggtat aaatatggta cattgcccga atatccggaa
     2641 tatcaaagat ttaaagtagt taaaaacata gactggcctc ctggagtaaa gattgagttt
     2701 tacgagatac cggcagagga tttgtggtaa aaattaacta ctgaatattt tgccaaatta
     2761 tagagtttgg gtcaagatga aatttatagt aactatcgcc aaaataaaaa aagccagtgg
     2821 gtgcttgcag cggtaacggc tgatgacggc actggcgcac ttgatgggg
//
LOCUS       DAOSOX010000260         2854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_35070_length_2854_cov_10.434084, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000260 DAOSOX010000000
VERSION     DAOSOX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35070_length_2854_cov_10.434084"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLX22_12085"
     CDS             <1..369
                     /locus_tag="PLX22_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOT20677.1"
                     /translation="RQSILANTLEAIIGAVYIDSGLTKSKKLVLRLLKKDIQRIDKLS
                     YLRDPKTTLQEIVQKKYKNRPIYTVIQESGPDHSKIYTVKLVINDEDITMGSGSSKRK
                     AESDAAKKALELIYSDRIHI"
     gene            366..830
                     /locus_tag="PLX22_12090"
     CDS             366..830
                     /locus_tag="PLX22_12090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT20678.1"
                     /translation="MRFKLSLSHKKIRIRVAGLVVKDDTILMIAHKKGNDIYWLLPGG
                     GVEYRESLDNALKREFKEELGLDVEVGDIAMIADSIEPNGRRHIINVIFWCNLVSESI
                     QLGNEKILYGYSFKPIQEIDTIVIYPPLNTCIIQLLKNKNEKIYKGSLWIPK"
     gene            815..>1131
                     /locus_tag="PLX22_12095"
     CDS             815..>1131
                     /locus_tag="PLX22_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20679.1"
                     /translation="MDSKINSETIVAHYSSKQSTITIGHGIFNFLVRLLPYSKWDVIG
                     AIISDRVYLLHRQTIDDSLQEINACIKGYDDTEYNKSYAYAESYFNYLLHNQFSRKSL
                     LIG"
     assembly_gap    1132..1231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1232..1594
                     /locus_tag="PLX22_12100"
     CDS             <1232..1594
                     /locus_tag="PLX22_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT20680.1"
                     /translation="RSILNYGHTVGHAIESVFHYTLSHGEAVAVGMLVESYISMKMGM
                     PQEDFELIKELLLYYKLLPNISFSTSDIINHMQYDKKNIKGTILFVLLEKVGKPVIGK
                     EVPGTLITEALNYYLTMV"
     gene            1599..1946
                     /locus_tag="PLX22_12105"
     CDS             1599..1946
                     /locus_tag="PLX22_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT20681.1"
                     /translation="MATEINYSIEEREGAKIIHLSGVLCSATEREFINLVESLIATNN
                     VIINFSATSMVTSSGLRALIDVSHLARQKKRRVMILGASENLHGLINVTESYDLLLIN
                     SVEEGLRKLEYYL"
     gene            complement(1959..2729)
                     /locus_tag="PLX22_12110"
     CDS             complement(1959..2729)
                     /locus_tag="PLX22_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT20682.1"
                     /translation="MLTLNNISSSLNNQQLLSHINAEFFEGEIIGIIGKPASGKSLLL
                     QVIAQHIQYDGHIMFNKKPIRAYTKRDLSAQIAFHPFSILQNTYLTVHDYLLLSRLPH
                     KKFLSPFSSYDHDVVNDYIALFELQPFVDVPIVTVSSDVMQRALLAFHFIRESILLML
                     DNPTQNLSLSSYTILHNAIIKYTSSGKNLCIIASHDLNFLTQTTDKLYIMDTGTIASW
                     YLPADINETIIKKYLDVDVFITRNIYNGKPNVHLYFES"
BASE COUNT          953 a          412 c          548 g          841 t
ORIGIN      
        1 aggcaatcca tacttgcaaa tacgcttgaa gctattattg gtgcagtata cattgattca
       61 ggtttgacaa aatcaaaaaa attggtgtta cgattgttaa aaaaggatat ccagaggatt
      121 gacaaacttt cctatcttcg tgatcccaaa acaacattac aggaaatagt ccagaagaag
      181 tacaaaaacc gaccaattta tactgttata caggaatcgg gacctgacca tagcaaaata
      241 tatactgtaa agctggtcat taatgatgag gatattacca tgggaagtgg gagcagtaaa
      301 cgcaaggcgg aatcagatgc tgctaaaaaa gcgttagagc tcatctattc tgacaggata
      361 cacatatgag gtttaagctg tcactatcgc ataaaaagat aagaattcgt gttgccggat
      421 tggttgtaaa agatgataca atactgatga ttgcacataa aaaagggaac gacatctatt
      481 ggttgctgcc tggagggggc gttgaatatc gggaatcact ggataatgca ttaaaaagag
      541 aatttaaaga agagctgggt cttgacgttg aggtcggcga tatagctatg attgctgatt
      601 ccatagagcc aaatggtagg cgacacataa taaatgtcat tttctggtgc aaccttgtaa
      661 gtgagtcaat acagcttggg aatgaaaaaa ttttgtatgg ctatagtttt aaacccatac
      721 aggaaataga taccattgtc atttatcctc ctcttaacac atgtatcatc cagcttttga
      781 aaaacaaaaa tgaaaaaatt tataaaggtt cattgtggat tccaaaataa atagtgaaac
      841 aattgtagct cattattcat caaaacaatc gacaataact atcggacacg gtatttttaa
      901 ttttcttgtt cgtttattgc catattcaaa atgggatgtt attggagcaa ttattagtga
      961 cagggtgtat ttacttcaca ggcaaactat tgatgatagc ttacaagaaa tcaatgcctg
     1021 cattaaagga tatgacgata cagaatataa taaatcatat gcgtatgctg aaagttattt
     1081 taattattta ttgcataatc aattttccag aaagtcatta ctcattggta tnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggtctatt ttaaactatg gtcatacagt
     1261 tgggcatgcc attgaatcag tgtttcacta tacgttaagt catggcgaag ctgtcgcggt
     1321 agggatgctg gtagagtcgt atatatctat gaagatgggg atgccgcaag aagattttga
     1381 actcataaag gagttgctcc tgtactataa gttgctgcca aacatttcat tttctaccag
     1441 tgatataata aatcatatgc agtatgataa aaaaaacata aaaggtacaa tcctctttgt
     1501 tcttctggaa aaggtgggca agccagtgat aggcaaggag gtgcctggca cattgataac
     1561 tgaagcattg aattattatt taacaatggt gtgatactat ggcgacagaa ataaactatt
     1621 caattgaaga aagagagggg gcaaaaatta ttcacctatc gggagtattg tgttctgcta
     1681 ctgaacgaga atttataaat ttagttgaaa gtttaattgc tacaaataat gttataatca
     1741 atttttccgc tacttcgatg gtaacatcat caggattaag agcattaatt gatgtatcac
     1801 acttagcacg gcaaaaaaaa cggcgtgtca tgatactggg tgcatccgaa aatcttcatg
     1861 gtttaataaa tgttactgag tcatatgatc ttttgctcat caatagtgtg gaagaaggat
     1921 taagaaaatt agaatattac ctgtaaactt gatgtaagtt acgattcaaa atataaatgt
     1981 acattgggct tcccattata gatattacgt gttataaata catcaacatc cagatatttt
     2041 tttattatgg tttcatttat atcagcagga aggtaccagc tggcaatagt tccagtatcc
     2101 ataatatata atttatctgt agtttgtgtt aaaaaattta aatcatgtga ggctataata
     2161 caaagatttt tccccgaaga tgtgtattta atgatagcat tatgcaatat agtgtatgaa
     2221 gataatgaaa ggttctgtgt gggattatca agcatcagta aaattgattc tctgataaag
     2281 tgaaatgcca ataaggcgcg ttgcattaca tcagatgata ctgtcacaat gggaacgtca
     2341 acaaatggtt gcagctcaaa caaggctata tagtcattta ccacatcatg atcatatgaa
     2401 gaaaatggtg ataaaaactt tttgtgaggt agcctgctca gcaacaaata gtcatgtacg
     2461 gtaaggtaag tgttttgaag aatactaaaa ggatgaaatg caatttgtgc ggataaatct
     2521 cttttggtat acgcccttat tggtttttta ttgaacatta tgtgtccatc gtattgtata
     2581 tgctgagcaa tgacctgtag caataacgat ttcccacttg ctggtttacc aataatgcca
     2641 ataatctcgc cttcaaaaaa ttcagcatta atgtgcgata gtaactgttg attgttcagt
     2701 gatgatgata tgttattcaa cgtgagcatg gcattctcct ggtaactatc gccaaaaaac
     2761 aaaagtcggt tatcgtaagt ggaataatga tagaaaaagg attaacaaag tatgatacat
     2821 acagtagcag tgttatgcat agtgctaaaa caat
//
LOCUS       DAOSOX010000261         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_35503_length_2826_cov_5.193793, whole genome shotgun sequence.
ACCESSION   DAOSOX010000261 DAOSOX010000000
VERSION     DAOSOX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35503_length_2826_cov_5.193793"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(450..2786)
                     /locus_tag="PLX22_12115"
     CDS             complement(450..2786)
                     /locus_tag="PLX22_12115"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOT20683.1"
                     /translation="MKKIIIILVVVIVILGAVIYLFLRTGLPDYTSSLQVPGLASPVT
                     IERNKFAVPTITAQNMDDLYFAWGYVNAQDRLFQMEFTKRVGQGRISEFAGEEALSKD
                     YFLRAVGFYERAKEYTKKLSPYYKRLCQRYADGVNYFIETKGRNAYMILLGMKKEKWE
                     IADSVLVGMMLNWSLAYNMKYELLYHKIVQKIGKEKASLLLNFVPPDTPTVIGDSSGK
                     WLDDAHVSSLLKEFDWLFGCRSASNNWAVAPQLTSHGKAILCSDMQVHQSKLPNDFYL
                     IHVKAGDFEVAGAQVTGLPFIASGFNKHCAWGLTNQGADMVDLFKERIDWKAKRYHYN
                     GKWLPLKNRKEVFAVKGKQPIVKTMYYAGKKPVLTEVFKDLNFDVSLDWTGFDAIALE
                     GFFMINAAKNFDEFMEGAKQIRMSPQNLVYADDKGNIAFRVIGSLPVRKKGTGNFIQD
                     GEKIYSNWNGNIPDDKYPILKNPERGYIATANNLNAADYPYELNGTYAPGYRYENIAT
                     MLRNKKDLDVNYMKKMQTDTHTVLAKKVKAILKKFIQRDALDAYMQKAFDRVIGWDAD
                     SSKDSIGASVYNTFYVRFAYQTLVDELGDTVTEEYVSERYISMERFFEMVEKGSVFFD
                     DVRTNKSETVADIAQRAFKETCQLLCDYFGTDNVEKWQWGKIHSIKFDHILGMSALLK
                     PLVNYGPFPFEGDGETNNRAKFLEVAPPFIADLASAPRIIVKFTPQPEASMMLITGEN
                     EHFLSKHNTDMVDAWLDHVYFELGKGEVAYSMELIPEK"
BASE COUNT          853 a          654 c          441 g          878 t
ORIGIN      
        1 cgttgtattg tccatataca acctgcatat cgatattcat attgtgcaag ctctgaacat
       61 cattccgagc accatcctga gcaaaaataa aagaaccaaa caccacaccc tgtagtataa
      121 gtgccatgaa tatataaaga caccttttgc tattatgtat attttccatg caaccccata
      181 ctgtatatgt aattcattaa ggatgaatac tatcgtaaaa atggaatgta atgaaggttg
      241 attattgaaa cagggatgtt ttaagtcaat acaaatatga acaagttttc atacaaacac
      301 ctgtttcatc aataattata ttgtaataac catgcatttc ccattgtaat gcttcattag
      361 ctcaattgtg gtgagcatca ttatctctta acacaataac tgccaataag ctattgtgct
      421 gtcacaagca ctatatgcac ttcaatgcgt tatttctcag ggatcaattc catagaataa
      481 gccacctctc ccttacccag ttcaaaatat acatgatcaa gccatgcatc aaccatatca
      541 gtattatgct tacttaaaaa gtgttcattt tcccctgtaa tgagcatcat ggatgcttca
      601 ggttgtggag taaactttac aataatacgc ggagctgaag ctagatcagc aataaacggg
      661 ggagcaacct caagaaattt tgcacggtta tttgtttcgc catcaccttc aaaagggaac
      721 ggaccataat tgacaagggg ctttaaaagt gctgacatcc caagtatatg atcaaatttt
      781 atgctatgaa tttttcccca ttgccatttt tcaacattat ctgtaccaaa ataatcacac
      841 agcaactgac atgtttcttt aaatgcccgt tgtgctatat ctgcaacggt ttcacttttg
      901 tttgtccgca catcatcaaa aaaaacactc cccttttcaa ccatctcaaa aaagcgttcc
      961 atgctgatat atcgctcact tacatactcc tctgtaactg tatcacccaa ctcatcaacc
     1021 agagtttgat acgcaaatcg cacataaaat gtgttgtaaa cagaggcgcc aatagaatct
     1081 ttagaactat cagcatccca tccaattacc ctatcaaatg ccttttgcat gtaagcatca
     1141 agtgcatcac gttgtataaa ctttttcagt attgctttaa ccttttttgc aagcactgta
     1201 tgagtatcgg tctgcatttt tttcatataa ttaacatcaa gatccttctt atttcgcagc
     1261 atcgtcgcaa tattttcata gcgatagccg ggcgcataag taccgttcaa ctcataagga
     1321 tagtcagcag cattgagatt atttgcagtg gcaatgtacc ctcgttcagg atttttcaat
     1381 atgggatact tatcatcagg tatatttcca ttccagttgc tgtatatctt ttctccatcc
     1441 tggataaaat tgccagtacc cttttttcgc accggcagtg agccaatgac cctgaatgca
     1501 atatttccct tatcatcagc ataaaccaga ttctgaggcg acatcctgat ctgttttgcg
     1561 ccttccataa attcatcaaa atttttagct gcatttatca taaaaaaacc ctctaatgca
     1621 attgcatcaa aaccggtcca atcaaggctt acatcaaaat tcagatcttt aaatacttca
     1681 gttaacacag gtttcttccc tgcataatac atggtcttta caattggttg tttccctttt
     1741 acagcaaata cctcttttct attttttaat ggcagccatt ttccattgta atgataacgt
     1801 tttgctttcc aatcaatcct ttctttaaaa aggtcaacca tatcggcacc ctgattggta
     1861 agcccccatg cacaatgctt gttaaatcct gaagcaataa acggcaagcc cgttacctga
     1921 gcacccgcaa cttcaaaatc gccagcttta acatggataa gataaaaatc gttgggaagt
     1981 tttgactggt gaacctgcat atcgctgcac agtattgctt tcccgtgcga tgtcagttgc
     2041 ggagccaccg cccagttatt ggaagcagag cggcagccaa aaagccaatc aaactctttc
     2101 agcaaagagc taacatgtgc atcatccagc cactttccgg aactatcgcc aataaccgtt
     2161 ggcgtatcag gtggtacaaa attcaaaagt aacgaggctt tctctttccc tattttctga
     2221 acaattttgt ggtacaacag ttcatacttc atattatatg ccagactcca gttaagcatc
     2281 atgccaacca gcactgagtc tgctatctcc catttttctt ttttcattcc cagtagaatc
     2341 atgtaagcat ttctgccttt tgtttcaata aaatagttaa ctccatcggc atagcgctga
     2401 cacaaccttt tataataggg cgatagtttt ttggtatatt cctttgccct ttcataaaat
     2461 cctaccgccc gcaaaaagta atctttactc aaagcttctt caccagcaaa ttcgctgatg
     2521 cgcccctgtc caactcgttt tgtgaattcc atctgaaaaa gcctgtcctg cgcattgaca
     2581 taaccccacg caaagtacag atcatccatg ttttgtgctg taatggttgg cacggcaaat
     2641 ttattgcgct cgattgttac cggagaagca agcccgggta cttgtaaact agaggtataa
     2701 tccggcaagc ctgttctcaa aaataaataa atgacagcac caagaatcac tataacaact
     2761 accagtataa tgattatctt tttcattgca aacccccatt gtttcatctg catctattat
     2821 attata
//
LOCUS       DAOSOX010000262         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_35576_length_2822_cov_7.951934, whole genome shotgun sequence.
ACCESSION   DAOSOX010000262 DAOSOX010000000
VERSION     DAOSOX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35576_length_2822_cov_7.951934"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLX22_12120"
     CDS             complement(<1..209)
                     /locus_tag="PLX22_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20684.1"
                     /translation="MSLLSITFFIVGASIVGALLVIIRAHQYPAKVRKAQEYLDNGDI
                     AKASEIVKLVLERKKDYIPARYLRA"
     gene            724..1254
                     /gene="purE"
                     /locus_tag="PLX22_12125"
     CDS             724..1254
                     /gene="purE"
                     /locus_tag="PLX22_12125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT20685.1"
                     /translation="MTEIGIIVGSDSDLPELKGCFQTLDDFNVSYDVKAYSAHRTPHL
                     VAEWVVQFEKDGGRVIIAAAGGAAHLPGVVASHTVLPVIGIPIGTSIAGGLDSLLSIV
                     QMPTGIPVATVATGKAGATNAALLAIAILSTSNPDYSTKLKNYRITMQKTITRKNETL
                     TKQGIYEYIKNLEAQK"
     gene            1251..2819
                     /gene="guaA"
                     /locus_tag="PLX22_12130"
     CDS             1251..2819
                     /gene="guaA"
                     /locus_tag="PLX22_12130"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT20686.1"
                     /translation="MIEHGIGISSQNKVLILDFGSQYTQLIARKIRELHVYAEIVPYT
                     ITIADIKKENPGAIVLSGGPSSLYEDNAPHISDDLFSLGLPVLGICYGLYAVVNAFHG
                     IINPSPFKEYGRATITLIEESPLFKDLPRTQTVWMSHGDTVERIPETFKTIARSENAP
                     HAAIENREKKIYGVQFHPEVYHTQYGKEILRNFLFTICNFQPTWTMQSFIDAAIDDIR
                     TMVKNGTVLLGLSGGVDSSVTAVLLQKAIGDRLYCVFVNNGLLRKDEHIKVIERFKKH
                     LKLNLIYADAEEHFLHKLKGVIDPEEKRKIIGKEFITIFKQEAAKIGTFDFLAQGTLY
                     PDVIESVSTKGPSDTIKSHHNRVPEVLELINEGKVIEPLKELFKDEVRELGRQLGMPE
                     ELINRHPFPGPGLAIRIIGEVTRERLAIVREADDIFIEEIKNAHFYNTLWQAFAVFLP
                     VKSVGVMGDKRTYDNVIALRAVTSLDAMTADWAYLPEDLMRRISTRIINEVKGVNRVV
                     YDISSKPPSTIEWE"
BASE COUNT          877 a          539 c          575 g          831 t
ORIGIN      
        1 tgcgccctga gataacgcgc tggtatataa tccttttttc gttcaagaac cagcttgaca
       61 atttcacttg cttttgcaat gtctccatta tcaagatatt cttgggcttt tcgcactttt
      121 gcaggatatt gatgtgcccg tataattacc agcagcgcgc caacaattga tgcgccaact
      181 ataaaaaatg taatgcttaa taatgacata gatttttcat attttacatt tgattttatt
      241 actacatgag ttatatttta agtcaagcat tatagcttta aaagcaaaaa atgatatgat
      301 agctttttaa cttatgaggt tatgtgtata atataacaca ttatatgcaa gtaagttttt
      361 catcatatca aagaggtaac tctaaaaatt aaaaatactt tcactttata attattagag
      421 gttcctttgt gcttatactg tactatggat caattccatg acagttttgt ttcaggatat
      481 cagttatgaa atagatgctg aaataagcct gccctggcga tagtccggga ttaatcatgg
      541 caatggcaag aaatatcatc ctgaactccg attcaggatc tcatggtgaa aaagagtttc
      601 cggatcaagt ccagtatggg aggggtgtat agtacctatt ttatgaggac acttcattcc
      661 tcgccttacg aaggagagag acagggggtg agggtaataa atttattttc catggaggaa
      721 tatatgacag aaatagggat tatcgttggc agtgattcgg accttcctga attaaagggg
      781 tgtttccaga cattagatga ttttaatgtt tcgtatgatg taaaagcgta ttctgctcac
      841 cgtactcctc atctggttgc tgaatgggtt gttcagtttg aaaaggatgg tggcagggtt
      901 atcattgcag cggcaggcgg cgctgctcat ctgccagggg ttgttgcttc tcatactgtc
      961 ctgccggtca taggcatccc cattggaaca tccatagcag gagggcttga ttcattgctt
     1021 tctattgtcc agatgccaac gggtataccg gtagcaacgg ttgctacagg aaaagcaggt
     1081 gcaaccaatg cagcacttct ggctattgca attctatcaa cctccaatcc tgactatagt
     1141 actaagttaa aaaattaccg catcacaatg caaaaaacta ttaccagaaa aaatgaaacc
     1201 ttaacaaaac agggtatata cgaatatatc aaaaacttag aggcacaaaa atgatagagc
     1261 atggcattgg aatatcctct caaaataaag tgctcatact ggactttggt tcgcagtaca
     1321 cacagctcat tgctcgtaaa atacgggaat tgcatgtata tgcagagata gttccctata
     1381 caataactat cgccgacata aaaaaagaaa atcccggtgc tattgtgctt tcaggcgggc
     1441 cttcctcact atatgaggat aacgccccgc acatctcaga tgatctgttt tcactgggat
     1501 tgccggtact tggtatttgc tatgggttat atgctgtcgt taatgcattt catggtatca
     1561 tcaatccttc tccattcaag gaatatggaa gggcaaccat aacgttaatt gaagaatctc
     1621 cattgtttaa ggatctgccc cgcacgcaaa cagtatggat gagtcacggt gataccgtag
     1681 aacgtattcc cgagacattc aaaaccatag cacgttccga gaatgcacca cacgctgcta
     1741 tagaaaacag ggaaaagaaa atttacggtg tccaatttca ccctgaagtg tatcataccc
     1801 agtacggaaa ggaaatttta cgtaattttt tatttactat ctgcaatttc cagcctacat
     1861 ggaccatgca atctttcatt gatgctgcga ttgatgatat acgcactatg gtaaaaaatg
     1921 gaactgtttt actgggactg tccggcggcg ttgattcttc tgttaccgca gtgttgttac
     1981 aaaaagctat cggtgatcgc ttatattgtg ttttcgttaa caacggcctt ttacgaaaag
     2041 acgaacacat aaaggttatt gaacgtttca aaaaacatct aaaactcaat ctcatctatg
     2101 ctgatgctga agaacatttt ttacataaac ttaaaggcgt tattgacccg gaagaaaagc
     2161 gtaaaataat tggcaaagaa tttattacta tttttaaaca agaagctgct aaaattggaa
     2221 cctttgactt tttagcacag ggtacactct atcccgatgt cattgaatca gtttctacaa
     2281 agggaccatc tgatacaata aaaagccacc acaacagggt gcctgaagtg ctggagctta
     2341 tcaatgaagg caaagtgatt gaaccattaa aagagctatt taaggatgag gttcgcgaat
     2401 tgggcagaca attaggaatg cccgaagaat taattaacag gcatccattc cccggtcctg
     2461 gtttagctat tcgcatcatt ggcgaggtta ccagagaacg tcttgccata gtgcgtgaag
     2521 ctgatgacat atttatagaa gaaattaaga atgctcattt ttataacaca ttgtggcagg
     2581 cttttgcggt atttttaccc gttaaatcgg taggtgtcat gggtgataaa cgtacctacg
     2641 ataatgtcat agcactgcgt gcagttacct cacttgatgc aatgacagcc gactgggcat
     2701 atctgcctga agatttaatg cgccgaatct ctacacgcat tattaatgaa gtaaaaggcg
     2761 tgaacagagt tgtatatgat atctcatcaa aaccaccatc aaccattgaa tgggaataaa
     2821 aa
//
LOCUS       DAOSOX010000263         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36007_length_2796_cov_9.722364, whole genome shotgun sequence.
ACCESSION   DAOSOX010000263 DAOSOX010000000
VERSION     DAOSOX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36007_length_2796_cov_9.722364"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..742)
                     /locus_tag="PLX22_12135"
     CDS             complement(206..742)
                     /locus_tag="PLX22_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20687.1"
                     /translation="MPATNKKQTAKKSVKKEVLHNNNTNNDNTRKYIKAINALLKKLD
                     EESLVFLYEQAKVLLHNLEVSKLQEEFEELDAKTITAKRYKFSKEKLEVIEADDLRHF
                     IFVINGARNFFSRDEMRTIVKLCHSAPSLKDGMRMLYNWFEKNRKDVIIDTEIDGPND
                     KALETIYNYIINHYTVSK"
     gene            1339..>2796
                     /locus_tag="PLX22_12140"
     CDS             1339..>2796
                     /locus_tag="PLX22_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT20688.1"
                     /translation="MKKAAAKKEEKTIAINPATGEVLGSFPINTVEDVKKAVEEARIA
                     QPLWQALPVKQRVHHIKKCIDFLQQNAEKIAKVISQDNGKTIVDALTAEIVPAVLATR
                     FYCKNAQKFLNDRKLPMGSILFVNKRSKIVRVPYGVVGIISPWNYPFSIPYSEIIMGL
                     LAGNAVIVKTASETQCVGHILKECIEAAELPPHIFTYINMPGSIAGDAFIEAGIDKLF
                     FTGSVRVGKYLMKKASERLTPLVLELGGNDPMIVCDDADLYRAAAGAVWAGMQNAGQS
                     CGGVERIYVDRKVYNKFLTILKGMVESLRVGDGSDISSDIGAMTTKRQMEVVQHHIDD
                     AVKKGATIYAKSKVNSKKGQFMPCMVLTDVNHDMLTMKDETFGPIVGVMPYDTIDEAI
                     ALSNDSYLGLTASVWSKNRSKAMKIGREIKAGAITINDHLMSHGLAETPWGGFKESGI
                     GRTHGDIGFAEMTQPQVIVNDILPFARRNFWWKPSD"
BASE COUNT          966 a          408 c          521 g          901 t
ORIGIN      
        1 acacattttg cttttataaa gaagaagata ttgtttgttg caaattttac tgggggccac
       61 acccggacat actacgaaaa gccaaacaac agcaataaat actatactga attctgattc
      121 aacctctaac gttaattatt ttgattccgg atcaagcccg taatgacgaa aggaataatc
      181 ctattttcaa taagaattgt ttgttctact ttgaaacagt ataatgattt ataatataat
      241 tgtagattgt ttctaaagct ttatcatttg ggccatcaat ctcagtgtca ataataacat
      301 cttttctgtt tttctcaaac cagttgtata acattcgcat tccatctttt aacgaaggag
      361 cgctatgaca taatttaaca attgtccgca tttcatcacg gctgaagaaa ttgcgggcac
      421 catttataac aaaaataaaa tgccgcaaat catcagcttc aataacttct aatttttctt
      481 tgctaaattt ataacgtttt gcagttattg ttttagcatc aagttcttca aattcctcct
      541 gaagtttgga tacttccaaa ttatgtaata aaacttttgc ctgttcatat aaaaatacaa
      601 ggctttcttc atctaacttt tttaatagtg catttattgc tttaatatac tttctggtat
      661 tgtcattgtt tgtgttatta ttatggagca cttctttttt tacacttttt tttgctgttt
      721 gttttttgtt tgttgctggc atataatacc ccctttactt tattagaaat gctttataac
      781 gttgcatcga aatgtataga aaaaatatac atttaataga ttctactcaa tcatgcttta
      841 tatgtaacta taaaaaatag catattggaa atttagaaat ttaattttgt caataaaaat
      901 tatgattata ataatggtaa tagggtaagt tcaataattg aagtatagag taattgtaat
      961 ttcgaaactt caaataacta aaaatacaag gaggctgtta ataattagat tttttacatt
     1021 cccttgcatg gaaaagtatt ataaacatat atcatcgtat tattgatatt ggaaaaactt
     1081 aattttaaaa atgtgtataa aacgatagtg tacataaaaa caacaattgt gtaatgacaa
     1141 tgctaaagaa aaaattaaca aaaatgaagt gactattgac agacaaataa aatattttat
     1201 ttcttgacaa atattgaatt actgacaata agtagtgttg attaaaataa gattcatttg
     1261 aatattatta aaaaataaag atagcaatta ttatacctaa atagataggg gtaaaattat
     1321 ttttaactgg aggaaaaaat gaaaaaagct gctgcaaaga aagaggaaaa aacaatagcc
     1381 atcaatcctg ccaccggtga agttttagga agtttcccca tcaacaccgt tgaagatgtt
     1441 aaaaaagcgg ttgaagaagc ccgtatagca caacctttat ggcaggcctt acctgttaag
     1501 caacgggtgc accatataaa aaaatgtatt gattttctac aacagaacgc agagaaaatt
     1561 gctaaagtaa tttctcagga taatggtaaa acaatagttg atgcattgac ggctgagata
     1621 gttcctgctg tgcttgctac aaggttttat tgtaaaaatg cacaaaaatt cctgaacgat
     1681 agaaagctac ccatgggttc tatattgttt gtcaataagc gaagtaaaat agtgcgtgtt
     1741 ccctatggtg ttgtaggtat tatatcacca tggaattatc cattttccat tccttattct
     1801 gaaattatca tgggattgtt agcagggaat gcagtaatag taaaaacagc ttcagaaaca
     1861 caatgtgtag gtcatatttt aaaagagtgt attgaagcag ctgaattacc ccctcatata
     1921 tttacctata tcaatatgcc gggtagtatt gctggtgatg cattcattga ggctggtata
     1981 gataaattgt tttttactgg ttcggtaagg gtgggcaaat accttatgaa gaaagcatct
     2041 gaacgattaa ctcctctggt acttgaactt ggtggcaatg acccaatgat tgtttgtgat
     2101 gatgccgatc tgtatagagc tgctgcaggt gctgtatggg caggtatgca aaatgcaggg
     2161 cagtcatgcg gaggggttga aaggatatat gtcgatagaa aagtatataa taaattcctt
     2221 acaatcttaa agggcatggt agaatcattg cgagttggtg atgggagtga tatctcatct
     2281 gatattggtg cgatgacaac aaagaggcag atggaggtag tacaacatca tattgacgat
     2341 gctgttaaaa aaggtgcaac catatatgca aaatcaaagg ttaatagtaa aaaaggccag
     2401 tttatgccat gtatggtact tacggatgtt aaccatgata tgctgacaat gaaagatgaa
     2461 acctttggac ccatagtagg cgttatgccg tatgatacaa ttgatgaggc aatagcgttg
     2521 tcaaatgatt cgtatcttgg attaacagcg tcagtatggt caaagaacag atctaaagca
     2581 atgaagattg gcagggaaat aaaagctggt gccatcacaa taaatgatca tttaatgagc
     2641 catggattgg ctgagactcc atggggtgga tttaaagaaa gcgggatagg aagaactcat
     2701 ggtgatattg gttttgcaga aatgacacag ccacaggtca ttgtaaatga tattttacca
     2761 tttgcacgca gaaatttctg gtggaagcct tctgat
//
LOCUS       DAOSOX010000264         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36108_length_2790_cov_5.263985, whole genome shotgun sequence.
ACCESSION   DAOSOX010000264 DAOSOX010000000
VERSION     DAOSOX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36108_length_2790_cov_5.263985"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLX22_12145"
     CDS             <1..301
                     /locus_tag="PLX22_12145"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOT20689.1"
                     /translation="CDPVHGNTYVNKYNRKVRRFEDIVKEIESFWEICIHEKVIPGGI
                     HIELTGDNVTECIGGLRALSEEDLDNNYTSLCDPRLNAEQSVELAFKIADIVKQY"
     gene            333..1508
                     /locus_tag="PLX22_12150"
     CDS             333..1508
                     /locus_tag="PLX22_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT20690.1"
                     /translation="MKKLLIAGGSHSDIPLILAAKKLGYFVITTGTREEDLGHQYSDK
                     YIKADYSNMEQIVSIAIDEKVDALCPCCNDFSAITCSYVAEKLGFKTFDTLEVTRTIH
                     YKNKFREFMKQYNFPSPVAANFDNVEDALQSIEALQLPLIVKPVDLTGGKGISRIATY
                     DEAKQAIIKALSISRQKKIVIEEFIDGSYHGLSVFLKNKKVAFYFFDNEYYYLNKYLV
                     AGAYCPGSVPKQAIQQLCAVYQKMAEILSLHDGIFHTQFILKDDKPYITEICRRPPGD
                     LYLKLVEYATGIPYSEYIVRAFAGLDITGIQQSPINGNFIRHCIMPNKCGIVKKIVYS
                     HEINNNILEKFEWYTTGETVTDYLTHKFGIVFLKFDSYNNMVDCINSINELIKVEME"
     gene            1556..2704
                     /gene="rffA"
                     /locus_tag="PLX22_12155"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             1556..2704
                     /gene="rffA"
                     /locus_tag="PLX22_12155"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOT20691.1"
                     /translation="MDTHNIFIPFNKPPHTGNEEQYIKQALQSGKISGDGKFGNLCEQ
                     WFQSQLQCKRTLLTSSCTHALEMCAMLIDTKPGDEIIMPSYTFVSTANAFVLRGATIV
                     FVDIRPDTMNIDETKIEKAITTKTKAIVVVHYAGVACAMDAIRNIAMKYNIPVIEDAA
                     QAMLSTYNGSHAGTLGQLGTFSFHESKNYTSAGEGGLLLVNHAQWIQRSEIIREKGTN
                     RSQFFRGMVDKYTWVDIGSSYLMNDISAAYLWGQLEKADDIFHNRMKTWNMYYESLLP
                     LNKKSIIALPIVPDECRHNAHMFYIKVDDLHVRTQLLKYLKNNGIYAVFHYVPLHSSP
                     AGQQYGRFDGTDEYTTKESERLLRLPLYYGMPEQHCKKVIDTIYQFFS"
BASE COUNT          962 a          505 c          535 g          788 t
ORIGIN      
        1 atgcgatcca gtccatggta atacctatgt taataaatat aatcgtaaag tgcggcgttt
       61 tgaagacata gtaaaagaaa ttgaatcatt ctgggaaatt tgtatacatg aaaaagttat
      121 cccgggaggc atccatatag aattgactgg cgataatgta actgaatgca ttggtggatt
      181 gcgtgcttta tcagaagaag atttagataa caactacact tcattatgcg atccaaggct
      241 taatgcagag caatctgttg agttagcctt taaaattgct gatattgtaa aacaatattg
      301 atgcttatag cttaaaaatc aggaatcaaa caatgaaaaa gttattgata gcaggaggaa
      361 gccattcaga tattccatta attttagcag caaaaaaatt gggctatttt gttattacta
      421 ctggcacgcg tgaagaagat ttaggacacc aatactctga taaatacatt aaagcggatt
      481 actcaaatat ggaacaaata gtatcgatag ccattgatga gaaagttgat gcgctatgcc
      541 cctgttgcaa tgacttttcg gcaataacct gttcgtatgt tgctgaaaaa cttggcttta
      601 aaacatttga tactcttgaa gtaaccagaa caattcatta caaaaacaaa tttcgcgaat
      661 ttatgaaaca atataatttt ccatcgcctg ttgcagcaaa ctttgataat gttgaagatg
      721 cactgcaatc aatagaagca ttacaattgc cgctcatagt aaagcctgtt gatttaacag
      781 gagggaaagg tatatccaga atagcaacgt atgatgaagc aaaacaagcc attattaaag
      841 cactatctat ttcccgtcaa aaaaaaatag taatagaaga atttattgac ggatcatatc
      901 atggactttc tgtgttttta aaaaacaaaa aggtagcctt ctattttttt gacaacgaat
      961 actattactt aaacaaatat cttgttgctg gcgcatactg tccaggaagt gtacccaaac
     1021 aagctattca acagctttgc gctgtgtatc aaaaaatggc ggaaattctt tctttacatg
     1081 atggtatttt ccacacgcaa tttattttaa aagatgataa accttacata actgaaatat
     1141 gcagaagacc acccggcgac ttatacctta aactggttga gtatgctacc ggtattccat
     1201 actctgaata tattgtccgc gcttttgcag gtttagatat taccggtata cagcaatcac
     1261 caattaatgg taattttata cgccattgta taatgcctaa taaatgtggt atcgtaaaga
     1321 agatagtcta tagccatgaa ataaacaata acatattgga aaaatttgaa tggtacacaa
     1381 ccggcgaaac tgttacggat tatttaactc acaaatttgg gattgtattt ttaaaatttg
     1441 attcatacaa taatatggtt gactgcatta atagtattaa tgaacttatt aaagttgaaa
     1501 tggaatagat agtagtatat taactacgat aacaataata aaataattta aaactatgga
     1561 tacccataac atctttattc cctttaataa accaccacat accggcaacg aagagcaata
     1621 cataaaacag gcattgcaaa gcggtaaaat ttctggcgat ggcaaatttg gcaacctgtg
     1681 cgagcaatgg ttccaatcgc aactgcagtg taagcgcaca ctgcttacct cttcatgcac
     1741 ccatgcattg gagatgtgtg caatgctcat tgatacaaag cccggagatg aaatcataat
     1801 gcccagctat acctttgtaa gcaccgccaa tgcctttgta ctgcggggtg caaccattgt
     1861 atttgttgat atacgtcccg ataccatgaa catagatgaa acaaagattg aaaaagctat
     1921 aaccacaaaa acaaaggcaa ttgttgtggt gcattatgca ggcgttgcat gcgccatgga
     1981 tgcaatacgt aacattgcca tgaaatataa tataccggtt attgaagacg cagcacaggc
     2041 tatgttgagc acctataacg gtagccatgc tggcacactg ggacagcttg gaacattcag
     2101 cttccatgaa agcaaaaatt ataccagtgc aggcgaaggc ggattactgc ttgtaaacca
     2161 tgcgcaatgg attcagcgtt cagagataat ccgtgaaaag ggcaccaacc gcagtcaatt
     2221 tttccgcggg atggtggata aatatacatg ggttgatatt ggctcaagct atcttatgaa
     2281 tgatatcagc gctgcatatc tgtggggaca actggaaaag gcggatgaca ttttccacaa
     2341 cagaatgaag acatggaaca tgtactatga atcgctgctg ccgcttaaca aaaagagcat
     2401 aatagcgttg ccgatagttc ctgatgaatg caggcacaat gcgcatatgt tctatatcaa
     2461 ggtagatgat ttgcatgtta ggacacagtt gcttaaatat ttaaaaaaca atggcatata
     2521 tgcagtattc cattatgtgc cgttgcacag ctcccctgcc ggacagcaat acggaagatt
     2581 tgatggcact gatgaatata ccacaaaaga aagtgagcgt ctactgcgcc tgcccctgta
     2641 ttatggaatg ccagagcaac attgcaaaaa ggttatagat accatctatc aatttttttc
     2701 ataaaaccat ctgctttata aaaatgtaac actatttaca ttctgaagac acgcagtact
     2761 tgtgtcagag catactatcc ttgaaggtac
//
LOCUS       DAOSOX010000265         2765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36544_length_2765_cov_5.684502, whole genome shotgun sequence.
ACCESSION   DAOSOX010000265 DAOSOX010000000
VERSION     DAOSOX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36544_length_2765_cov_5.684502"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1028
                     /locus_tag="PLX22_12160"
     CDS             351..1028
                     /locus_tag="PLX22_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOT20692.1"
                     /translation="MYTIHIFFLFAFGLFIGFVSSVPVGAVQLEVIKKAINGHLKPAI
                     SVALGSVTSDLIYGILVLFGIGNFLHQHNVQIVTYSLGIIVIIALLYRSYREHKHIPP
                     HLKTHIHYKKRTSYLTGFTIAITNPGMIVWWVIGYKLLLDLALFNTMTVPLKITFLIA
                     CCSGLAGYLILMAFMINKLQESISDKLLDRMNIVIMFLLVLVIIYFSVKLISLITNTS
                     TDIIHNF"
     gene            1108..1554
                     /locus_tag="PLX22_12165"
     CDS             1108..1554
                     /locus_tag="PLX22_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20693.1"
                     /translation="MDITVTQQFITDLQSLRENVPLEKEFFAFLYDYFIDAGMSMLLS
                     TPHDYCLIYFDLPEAIDDIQPSNVSWLVEKEEKFLSLTMFANGNQIQTYHTFHIAKNP
                     VNAFFLRSLKDSGTITIHFFTLIYGNMYKLKSIAFHLPEVIINQLE"
     gene            1562..2137
                     /locus_tag="PLX22_12170"
     CDS             1562..2137
                     /locus_tag="PLX22_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20694.1"
                     /translation="MREEIKEKFEHIKKGEPIPETNPRKKRFSRIFLLMNLIVFLILL
                     LLIFQKKEHSDYLYQTVLINNIQVRFSIIHSINKPILATITLTSPDKSVLHIKKPFAT
                     ITILHQNTEITSVPVYIDSTVLHIAPDKPITHTVSIPLDAIIAFGKKHDLLKYKRYAY
                     HSVIPLTVMFTLHTIMDYSTSLQCKCKVTAK"
     gene            2134..2580
                     /locus_tag="PLX22_12175"
     CDS             2134..2580
                     /locus_tag="PLX22_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOT20695.1"
                     /translation="MNRPTWDEYFMMIAHDVSSRATCIRRKVGAVIVKEKRILSTGYN
                     GVPTGITHCTPETCLRNIYNVPSGERHELCRGLHAEQNAIIQAAYHGVSIADAIIYVT
                     HQPCSICTKMLINAGIKTFIFEHPYNDELAKAMMDEAGIETIMFKK"
     gene            complement(2581..>2765)
                     /locus_tag="PLX22_12180"
     CDS             complement(2581..>2765)
                     /locus_tag="PLX22_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20696.1"
                     /translation="DLKKLSNTDTIKKLLQDDITIGGKLGIHATPTIIINKTMLKPGI
                     PPAYILEMAIKIEMQQ"
BASE COUNT          868 a          538 c          462 g          897 t
ORIGIN      
        1 ttggctttgc atcaaaaaac tgcattccca tcattgacaa ttgcaaggtg tataacggtt
       61 caagcgttgt ttttgaagat gacttttcta aagaatcaat acgaaagatt atattaaaag
      121 ggaaaaagct aacaccacag gaaatagagg aacaaaagaa gaaacaaaat aaatgacatg
      181 ttttaactat gtgttttgct tctatggctc ttctatggtt gtcgaagcac tataaacata
      241 cagaaaatat ccattaaaaa tgtattttgt aaaaaattaa aaaatacttg caacttttta
      301 tacataattt gtaccgtata gattatgatt tcattctgat aaggtatggt atgtatacaa
      361 ttcacatctt tttccttttt gcatttgggc tttttattgg gtttgtttct tcagtacctg
      421 ttggtgccgt tcaactggag gttataaaaa aagccattaa tggacatctt aaaccggcaa
      481 tctctgtggc gctgggttcg gttacatcgg accttattta cggcatcctt gttctttttg
      541 gcattggcaa ctttttgcat cagcataatg tgcagattgt tacctactcg ttgggcatca
      601 ttgttatcat agcattgcta taccgttcat atcgcgagca caaacatatc ccacctcatc
      661 ttaaaacaca tatacattat aaaaagcgta catcgtattt aaccggcttt actattgcca
      721 taaccaaccc cggtatgatt gtatggtggg ttattggtta caaacttctt ctggaccttg
      781 cactttttaa cactatgact gttccattga agataacatt tttaattgca tgctgcagcg
      841 ggcttgcagg ttaccttatt ctgatggctt ttatgataaa caaattgcag gaatcaattt
      901 cggataagct gcttgaccgc atgaacatag tcattatgtt tttgcttgta ctggtaataa
      961 tttatttcag cgttaaactt attagcctga ttaccaatac cagcactgat atcattcata
     1021 atttttaggg cgatagttac tgttaatgct ttcggttttg ttcaatacgg ttatacgtta
     1081 atactatatg gcaggcaata cccaattatg gatattactg taacacaaca atttattacc
     1141 gatttgcaaa gcttacgtga aaatgttcct cttgaaaaag agttctttgc ttttctgtac
     1201 gattacttca tagatgcggg aatgtccatg cttttgtcca caccccacga ttactgtttg
     1261 atctactttg atctaccgga agctattgat gatatacaac cttccaatgt ttcatggctt
     1321 gtggaaaaag aggaaaaatt tttaagctta accatgtttg ccaatggcaa ccagatacaa
     1381 acctaccata cattccatat tgcaaaaaat ccagttaatg catttttttt acggtcatta
     1441 aaggacagcg gtacaataac cattcatttt tttacattaa tatatggcaa catgtataaa
     1501 ctaaaatcaa ttgcatttca tcttcctgaa gtaatcatca atcaattaga atagtaaaac
     1561 catgcgtgaa gaaattaaag aaaagtttga acatataaaa aaaggggaac ccatccctga
     1621 aacaaatccg cgtaaaaaga gattttcacg aatatttttg ctcatgaacc ttattgtatt
     1681 tttgatatta ctattgctta tctttcaaaa aaaagagcat tcagactacc tctatcaaac
     1741 tgttttgata aacaacattc aggttcgctt ttcaatcatt cattcaatca acaagcccat
     1801 actggcaacc attacactga caagccctga caaatcagtt ttacacatta aaaaaccgtt
     1861 tgccacaata accatactgc accaaaatac tgaaattacc tcggtgcctg tgtacattga
     1921 ttcaactgta ctacatattg cacctgataa accaattact cataccgttt ctattcctct
     1981 tgatgcaatt attgcatttg ggaaaaaaca tgatttgctt aaatacaagc gctatgctta
     2041 tcacagcgta atccctttaa ccgtgatgtt tacacttcac acaataatgg attattcaac
     2101 gtcattacaa tgtaagtgta aggtaacagc aaaatgaacc gaccaacctg ggatgaatat
     2161 tttatgatga tagctcatga tgtttcgtca cgtgcaacct gcatacgacg caaggttggc
     2221 gcggttatag taaaggaaaa acgaattctt tcaaccggtt acaatggagt tccaaccgga
     2281 ataacccatt gcacccctga aacgtgtctt cgcaatatat acaatgtacc atcgggcgag
     2341 cgccatgagc tatgccgcgg cctccatgcc gagcaaaatg ccatcataca ggctgcctat
     2401 catggtgtat ccattgccga tgcaattatc tatgtcaccc atcagccatg ctctatctgc
     2461 acaaagatgc ttatcaatgc cggcataaag acatttatct ttgaacaccc ctataacgat
     2521 gagctggcaa aagcaatgat ggatgaggca ggtattgaaa caattatgtt taaaaaataa
     2581 ttattgttgc atctcaatct tgattgccat ctctaaaata taggctggcg gtatccccgg
     2641 ttttagcata gttttgttaa tgatgatggt tggcgttgca tgtatgccaa gtttaccacc
     2701 tattgttatg tcatcctgta aaagcttttt tattgtatca gtgttcgata gtttttttaa
     2761 atcag
//
LOCUS       DAOSOX010000266         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36694_length_2757_cov_8.386010, whole genome shotgun sequence.
ACCESSION   DAOSOX010000266 DAOSOX010000000
VERSION     DAOSOX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36694_length_2757_cov_8.386010"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /gene="ccsA"
                     /locus_tag="PLX22_12185"
     CDS             complement(<1..881)
                     /gene="ccsA"
                     /locus_tag="PLX22_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT20697.1"
                     /translation="MNSLYYLPWITALLIMLSTMAAVYSLSKPKIFTLAKYLYYAATG
                     IVALMLICLLMAFVTFKFQYSYVYHYSSVDLPVLYRIAGVWAGQEGSIVLWTFFIMIA
                     GVIAVTREEQLKQGFLTIVGLVSLIFVIHCINNNPFTYIWDTSKNFPVNIIPTDGAGL
                     NPLLQDFWMIIHPPVLFIGYALSTIAFIYAILSLLYNNYSIILTAYRYVLATVLTLGA
                     GIFLGGYWAYKVLGWGGYWGWDPVENASLIPWLISIALLHGLILQKRKQVLVRANIAM
                     AIGVYVFVLFGTFLTRSG"
     gene            complement(882..1253)
                     /locus_tag="PLX22_12190"
     CDS             complement(882..1253)
                     /locus_tag="PLX22_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOT20698.1"
                     /translation="MAKSKIIIVAIIVICSAVALFSFKGLLTPYVPFDDAKNNPDYVQ
                     IIGRLVKTVPMQNTTDGFLFVIEDNALERMTVLYRGAKPLNFEHATSVVAIGIYNNKK
                     QMFEADKILVKCPSKYTREQK"
     gene            complement(1240..1413)
                     /locus_tag="PLX22_12195"
     CDS             complement(1240..1413)
                     /locus_tag="PLX22_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HOT20699.1"
                     /translation="MKLYAIVTAMHFLAQTDSNYSKTLFWVSAVVWCGIVCYLVVLHR
                     KIKQLEKRVNGKK"
     gene            complement(1415..2197)
                     /gene="ccsA"
                     /locus_tag="PLX22_12200"
     CDS             complement(1415..2197)
                     /gene="ccsA"
                     /locus_tag="PLX22_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOT20700.1"
                     /translation="MFSTAGFFSIKRYNFSTCIFNSSWNGIIFIISIYLARILIVTRI
                     AMIVILLNGIVMMLSLYLIYIWVPPAAILGETSRIVYLHVPLAWVSCLAFAVAGIASL
                     LFLKKSKINYIVYARSSAHIGLFFSILTVLTGSLWAKIMWGSYWNWDPRETSITLLLL
                     LYIAYFSLQQIVSGNPKAPMLESVYLIIAFVVSPFLMFVAPRLYPTLHPEPVINAQGQ
                     LMLDTEMRLVLGISLVSFTLLYYILLGLWVRIHVLKSKLHEE"
     gene            complement(2080..2742)
                     /locus_tag="PLX22_12205"
     CDS             complement(2080..2742)
                     /locus_tag="PLX22_12205"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOT20701.1"
                     /translation="MIKALWRNIQKDILIEFRSGIIVSIVLSFSVIVTISIGFATGGI
                     INSPLLHAVILWIIIFFSGMNTLSHVFFREIEESTALFGVIHYPAAVIYLSKLLINCC
                     VMVVIAIMVIIIYTFVISVSVYYGVYLIAILIAGSLSLAFATTVIGAIAALAGARGGL
                     FTILAFPVVLPLFTVAIQSTARCFQQQVFSPLSAIIFLLAFSIVLGMVSYLLFPYIWR
                     EF"
BASE COUNT          965 a          573 c          472 g          747 t
ORIGIN      
        1 ccacttcgtg ttaaaaatgt gccaaaaagg acaaaaacat atacccctat tgccatggca
       61 atattagccc gtaccagaac ctgttttcgt ttctgcagta ttaatccatg caatagtgca
      121 atactgataa gccatggtat aagggatgca ttctcaactg ggtcccaacc ccaataacca
      181 ccccacccca ataccttgta tgcccagtag ccacctaaaa atatcccggc tcccagggtc
      241 aacacggttg cgagcacata acggtaagcg gtaagtataa tggaatagtt attatatagt
      301 aacgatagta ttgcataaat aaacgctatg gtagataatg catagccaat aaaaagaacc
      361 ggtggatgaa tgatcatcca gaaatcctgt aatagtggat taagcccggc accatctgtg
      421 ggaattatat ttacgggaaa attttttgat gtatcccata tataggtaaa cggattatta
      481 ttgatacaat gaataacaaa gataagcgat actaaaccca ctattgttaa aaagccttgt
      541 ttcaattgtt cttccctggt aacggcaata accccggcaa tcattataaa aaatgtccat
      601 aagacgatgg aaccttcctg acctgcccat accccggcaa tacggtatag tacgggcaaa
      661 tcaaccgatg aatagtggta tacataagaa tattgaaatt taaaggtaac aaatgccatt
      721 aacaaacata taagcattaa tgctacaatc cctgttgcag cataataaag atattttgct
      781 aaagtaaata tttttggttt gctcaatgaa tatacagctg ccattgtcga tagcataatc
      841 aatagtgccg taatccatgg cagataatat aaactattca tctatttttg ctccctggta
      901 tactttgatg ggcatttaac caatatttta tctgcttcaa acatctgttt tttgttatta
      961 tagatgccga ttgcaacaac gctggtagca tgttcaaagt tcaacggttt ggcacccctg
     1021 tatagtaccg tcatccgttc caatgcatta tcttctatca caaatagaaa accatctgtg
     1081 gtattctgca taggcactgt tttaaccagc ctgccaatta tctgaacata atcgggatta
     1141 ttctttgcat catcaaaggg aacatacggc gttaaaagcc ccttaaacga aaaaagtgct
     1201 acagcagaac atataacaat gattgccaca ataatgatct tactttttgc cattgaccct
     1261 cttttccagc tgttttattt tacggtgcaa tacaacaaga tagcagacta ttccacacca
     1321 tacaaccgca gatacccaga aaagcgtttt tgaataattt gaatctgtct gcgccaaaaa
     1381 atgcattgcg gtaactatcg catataattt catactattc ctcgtgcaat tttgatttca
     1441 gtacatgaat ccgcacccac aatccaagca atatgtagta caaaagcgta aatgatacca
     1501 atgatatacc aagcaccaac cgcatttcag tatccagcat caattgtccc tgagcattaa
     1561 taacaggttc tggatgcagc gttgggtaaa gccgtggtgc tacaaacatt aaaaacggtg
     1621 atacaacaaa cgcaattata agatacacac tttctaacat aggggctttt ggattaccgc
     1681 taacaatctg ttgcaggctg aaataggcaa tgtacaataa aagcaataat gtgatggagg
     1741 tttcacgcgg gtcccagttc cagtaactgc cccacataat ctttgcccac aacgagccag
     1801 taagtacagt aaggatgcta aaaaaaagac ctatgtgtgc agaacttcgt gcatacacta
     1861 tatagtttat ttttgatttt ttcaaaaaca atagtgaagc aatacctgct actgcaaaag
     1921 ccaaacaaga tacccatgca agtggcacat ggaggtatac tatacggctt gtttctccta
     1981 atatagccgc gggaggcacc cagatataga ttaaatacaa tgatagcatc atgacaatgc
     2041 catttaaaag tattacaatc atagctattc tcgttactat taaaattcgc gccagatata
     2101 tggaaataat aaatatgata ccattccaag aactattgaa aatgcaagta gaaaaattat
     2161 agcgcttaat ggagaaaaaa cctgctgttg aaaacacctg gctgttgatt gtattgcaac
     2221 agtaaaaagc ggtaatacta ccggaaaagc caatatggta aataatcctc cccgtgcgcc
     2281 ggctaatgct gcaatggctc ctataaccgt tgttgcaaaa gcaagcgata aacttcccgc
     2341 aatgagtatt gctataagat agacaccgta atacacagaa actgagataa caaaagtata
     2401 gataatgatg accataatgg caataacaac cattacacaa caattaatca agagttttga
     2461 aagatagata acagcagccg gataatgtat tacaccaaac agtgcagttg actcctctat
     2521 ctccctgaaa aaaacatgcg atagtgtatt catcccggaa aaaaaaatga ttatccatag
     2581 tataacagca tgcagtaaag ggctattgat gataccacct gttgcaaatc caatggaaat
     2641 agtaacaatg acactgaatg ataaaacaat tgaaacgata atcccacttc tgaattcaat
     2701 caatatatcc ttctggatgt tccgccataa tgccttaatc aagcactata ctccttt
//
LOCUS       DAOSOX010000267         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36732_length_2755_cov_7.399630, whole genome shotgun sequence.
ACCESSION   DAOSOX010000267 DAOSOX010000000
VERSION     DAOSOX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36732_length_2755_cov_7.399630"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..842)
                     /locus_tag="PLX22_12210"
     CDS             complement(6..842)
                     /locus_tag="PLX22_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOT20702.1"
                     /translation="MKRYNDFEDLSINQYFNAINQVELLTQEEEIDLAKKKGIGDEKA
                     KESLINANLRLVVKIAKNYTNLESSLIDLIQEGNLGLIRAAEKFDYNMGCRFSTYASY
                     WIKHYITRFIAKRSRTIRVPIRKGDLFKKIKRVQDELTNELGKEPSYRDIADMLGIDE
                     RSVSDILEVFQPTVSLEYPLNDDEFNLYEVVSDEKFLSPDTSISRRDLKKELKSAMES
                     LMENERLILTKRFGLDGSEPVTLKDVGNEFGISAETVRQIEARAMQKIKQRFSYLADY
                     IE"
     gene            complement(1296..1496)
                     /gene="rpmE"
                     /locus_tag="PLX22_12215"
     CDS             complement(1296..1496)
                     /gene="rpmE"
                     /locus_tag="PLX22_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT20703.1"
                     /translation="MKKGIHPEYFDTVIKCACGNEIHTRSTVKDLHVEICSNCHPFYT
                     KKQKIIDTTGRVEKFKKKYNIK"
     gene            complement(1533..2750)
                     /gene="rho"
                     /locus_tag="PLX22_12220"
     CDS             complement(1533..2750)
                     /gene="rho"
                     /locus_tag="PLX22_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT20704.1"
                     /translation="MSALMEIARSMGIEGISGLKKQDLIFEILKAQTERSGLIFSSGV
                     IEVLNDGYGFLRSPNYSYLPGPDDIYVSPSQIRLFGLRTGDTVAGQIRPPKDNERFFA
                     LLRIEAVNYENPEKLQNRVLFDNLTPLYPETRINLETTPEEIDMRILNIIAPIGMGQR
                     ALIVAPPRTGKTIILQKIANAITTNNPDIILIVLLIDERPEEVTDMERSVKGEVISST
                     FDEPASRHVQVSEMVLEKAKRLVEHKKDVVILLDSITRLARAYNQVVPTSGKILSGGV
                     DSNALHKPKRFFGAARNIEGGGSLTIIATALIETGSRMDEVIFEEFKGTGNCEIVLDR
                     KLADRRIFPAIDINKSGTRKEELLVDAEELNKIWILRKVLSSMSSTESMEFLIEKMRS
                     TKNNRAFFKAMQG"
BASE COUNT          832 a          553 c          443 g          927 t
ORIGIN      
        1 agaaattatt caatataatc agctaaatac gaaaatcgct gcttaatttt ctgcattgct
       61 cgggcttcta tctggcgaac tgtttcagca gaaataccaa actcatttcc gacatccttt
      121 aaagtaacag gttcactccc atcaagccca aacctctttg ttaagataag ccgttcattc
      181 tccattaatg attccattgc tgactttagt tcctttttta aatctctgcg tgaaatgctg
      241 gtgtcaggag aaagaaattt ttcgtcgctt accacctcat aaagattaaa ttcgtcgtca
      301 ttaagcggat attctaatga aacggtaggt tggaatacct ctaatatatc gcttacactt
      361 cgttcatcaa tacccagcat atctgcaata tctctataag aaggttcttt ccctaattcg
      421 ttggtaagct catcctgaac ccgtttaatc tttttaaaga gatcgccctt acggatagga
      481 acacggatag tgcggcttcg ttttgcaata aaacgtgtaa tatagtgctt gatccagtat
      541 gaagcatatg ttgaaaaacg gcagcccatg ttgtaatcaa acttctctgc tgctcttatt
      601 aaacctaaat tgccctcctg aatcaggtca ataagagatg actctaaatt tgtataattt
      661 tttgcaatct ttacaaccag tcgcaaatta gcattaatca acgattcctt tgctttttca
      721 tcaccgatgc cttttttctt tgctaaatca atctcttctt cctgagtgag aagttcaacc
      781 tgattaatgg cattgaaata ttgattgata gataaatctt caaaatcgtt atatcttttc
      841 atggctgcct cctatccatg atttcctcat cactatattt taaagcaatt tatgtgccaa
      901 atacttcatt tttttttaat attacaaaaa taattcaaca ggaataaaaa aatattgggt
      961 attcctgcgt attttttatt atatagtaat gaaaaaatct tgaaagttac aaataaatta
     1021 gtacactatt aaaatataat ataattagct tctaaaatac ttgataattg gtatagatag
     1081 tgttcattac agttacaatt gtgcctaaaa acaaagaata aagtcaacaa gtgtataata
     1141 aatacacata gacatagctt agtgtgtatt aattacacat aatagaacat ttttgccaat
     1201 ataccattaa taaataaacg cactcactac aagcgcgtta attgttataa ctgcatggta
     1261 tctatccgaa ggatagaata tcccattcct gtagattatt taatgttata tttcttctta
     1321 aatttttcta cacgtcctgt tgtgtcaata atcttttgct tcttggtata aaacggatga
     1381 cagtttgaac atatttcaac gtgcagatcc ttaaccgttg aacgagtgtg aatctcattc
     1441 ccacatgcac actttatcac agtatcaaaa tattcaggat gtataccttt tttcataata
     1501 actatctcct tatgccaaat aattatatcg cactatccct gcattgcttt aaaaaatgct
     1561 ctattatttt ttgtactgcg cattttctca attaaaaatt ccatggattc cgttgaactc
     1621 atagacgata atacttttcg caaaatccat attttattaa gttcttctgc atctacaagc
     1681 aactcttcct tacgggttcc ggacttgtta atatcgattg cggggaatat tcttcgatca
     1741 gctaattttc tgtcaagcac gatttcacag tttccagtac ctttaaattc ctcaaatatt
     1801 acttcgtcca tacggctgcc agtttctata agtgctgttg ctatgatggt taagctccca
     1861 ccgccttcta tattacgggc tgcaccaaaa aaacgttttg gtttatgcag ggcattggaa
     1921 tcaaccccac cggaaagtat ctttccgctt gttggcacca cctggttgta tgctcgtgct
     1981 aaacgggtaa tcgaatcaag tagtataaca acatcttttt tatgctcaac aaggcgcttt
     2041 gccttttcaa gtaccatctc actaacctga acatggcgtg atgcaggttc gtcaaacgtt
     2101 gatgaaataa cttcaccttt gaccgaacgt tccatatctg taacctcttc aggacgctca
     2161 tcaatgagca atacaatcaa gataatatcg ggattgtttg tggtaatagc atttgcaatt
     2221 ttttgtaaga taatggtttt gccggtacgc gggggtgcaa cgataagtgc acgttgaccc
     2281 atgccaatag gtgctatgat gttcagaata cgcatatcaa tctcttcagg tgttgtttca
     2341 aggttaatcc gtgtttcggg atataatggc gtaaggttat caaaaaggac cctgttctgc
     2401 aatttttcag gattttcgta attgacggct tctatcctta aaagggcaaa aaagcgttca
     2461 ttatctttcg gtgggcgaat ctgccctgca acagtatcac cagttcgtaa tccaaaaagc
     2521 cgtatctgag aaggggaaac atatatatcg tctggtcctg gaaggtaact atagtttggc
     2581 gatcttagaa aaccataccc atcattaaga acttcgataa caccgctgga aaatattaat
     2641 ccgctccgct cagtttgagc ttttaaaatt tcaaaaatca aatcctgctt ttttagtccg
     2701 gaaattcctt caatgcccat tgatcgggca atttccatga gagcacttat tgttt
//
LOCUS       DAOSOX010000268         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36733_length_2755_cov_5.739259, whole genome shotgun sequence.
ACCESSION   DAOSOX010000268 DAOSOX010000000
VERSION     DAOSOX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36733_length_2755_cov_5.739259"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLX22_12225"
     CDS             complement(<1..1078)
                     /locus_tag="PLX22_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HOT20705.1"
                     /translation="MKQSIAQTGLSLITKLGYAVGDFGANMVFQSVVLFLMFYLTDVF
                     LIPAGVAGTIFLIAKIWDAVSDPMMGYISDRTKSRWGQKRPYLLFGALPLGICMMLLF
                     YTPALEGSSKTMYALVMFLLVCTAYTVVNIPYGALTANLTLDTNERSRLTGYRMFFAI
                     LGTLFVAGATKPLVGLFDSSVEGWRFIGMLYGAIAAFFTLVTFATVKERIQHKDTDEY
                     HLRDIATTLKVNKPFIFLSIGVFLHLCAIGVLASMVNYLLKYNYAKEEFVPVAFLCMF
                     ATAAVAIPLWVFLSQKMGKKHAFNAGMGMLAVILLVLFFVKEFNSVLFIVLFVIAGIS
                     ISTVFFSPWAMIPDTVEYSQWKTGI"
     gene            complement(1096..2598)
                     /locus_tag="PLX22_12230"
     CDS             complement(1096..2598)
                     /locus_tag="PLX22_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT20706.1"
                     /translation="MKSIQIGFIGLGRIADLHYLGYTNNKQAKVYAVCDSNLELAQKR
                     KKQWNAEKVYTDYRDLVADAHVDAVEILTPHSLHESMTVAAAHAGKHIALQKPMTVDL
                     KSSDRIIAATDTAGKIFKVTDNYPFYPPIRLAKKIIDNGEIGKPIALRIKFIGGGSGG
                     WHVPPEAWQWRIKENTESGGMRGLDTFDHGHHLWTTAWYLCGGVEKTAGWIDSIDGII
                     DAPAVMMWKYDNGCYGSIEFVHLPSLTIPCKYYANDEWIEVSGTHGIVVIRRCTGSIN
                     KGPVVGVFTNKGWKNYNVPSDWSLGFVGATHNFINAILGKEEPMLSGRDAREILRFSL
                     AIQKSARLNREVYTHELDSKFPSLYYVRHHCKELKAKKPKKSIAERLGLSANFAKYAP
                     QAKELTRQFIKRYNPEAAKQWTVTIALHIKKEGNADEFLYSIAINNGALMVKEGALPE
                     KWDMKITAQAGVWAAILLKKKRIEMAYLQGLLKIEGKSEEALRLRSAFGI"
BASE COUNT          796 a          664 c          514 g          781 t
ORIGIN      
        1 gtatgcctgt tttccactgc gagtattcaa cagtatcggg gatcattgcc catggactga
       61 agaacacggt ggagatgctg atacccgcaa tgacgaatag cacaataaaa agaacactat
      121 taaattcctt aacaaaaaac aaaacaagca gtataacagc aagcatcccc atacctgcgt
      181 tgaatgcatg ttttttgccc atcttttgcg ataaaaaaac ccatagaggt attgccaccg
      241 ccgctgttgc aaacatacat aaaaatgcaa ccggaacaaa ttcctctttt gcataattat
      301 acttgagcag ataatttacc atcgatgcca gcacgcctat tgcacacaaa tgtaaaaaca
      361 cacctatcga taaaaaaata aacggtttat ttacctttaa cgttgtggca atatcgcgca
      421 gatgatattc atcggtatcc ttatgctgta tgcgctcctt tactgtggca aatgttacaa
      481 gggtaaaaaa tgctgctatg gcgccgtaca acattccaat aaagcgccac ccttcaacgg
      541 aactatcgaa caaaccaaca agaggctttg ttgcccctgc tacaaataat gttcccagta
      601 ttgcaaaaaa catccggtaa ccggtaaggc gtgagcgttc attggtatca agggtaaggt
      661 ttgctgtcaa agcgccgtaa ggaatattaa caacggtgta cgcagtgcat accagcaaaa
      721 acatcaccag cgcatacatt gtttttgatg aaccttcaag cgcaggagta taaaaaagta
      781 gcatcataca aatacccaga ggcagcgcac caaaaagaag atatggccgc ttctgccccc
      841 accgtgattt tgttctatcg ctgatgtacc ccatcattgg gtcgcttact gcatcccata
      901 tctttgcaat taaaaaaatt gtgccggcaa ctcccgcagg tatcaaaaaa acatctgtta
      961 aataaaacat taaaaaaaga actaccgact ggaacaccat gttggcgcca aaatcaccaa
     1021 cggcatatcc taattttgtt atgagtgata aaccggtttg tgctatactc tgtttcatac
     1081 gaaacctccc ctcaatcaaa ttccaaatgc tgaacgcaat cgcagcgcct cttccgactt
     1141 cccttcaatt ttcagcaacc cctgaagata cgccatttca atgcgtttct ttttgagtaa
     1201 aattgcagcc catacgcctg cctgcgcggt aatcttcatg tcccattttt cagggagcgc
     1261 cccttccttt accatcaacg cgccattatt aattgcaatg ctgtacagaa actcatcggc
     1321 attgccctct ttttttatat gcagggcgat agttactgtc cactgttttg ctgcctcggg
     1381 attataccgc tttataaatt gccgggtcag ctcttttgcc tgtggcgcat attttgcaaa
     1441 gtttgccgat aagccaaggc gctcagcaat gctttttttg ggcttttttg ccttgagctc
     1501 tttgcagtgg tggcgaacat agtaaagtga gggaaatttg ctgtccagtt cgtgtgtata
     1561 aacctccctg ttaagccggg ccgatttttg aattgctaaa gaaaagcgca aaatttcacg
     1621 tgcatccctt cctgaaagca tgggctcttc tttaccaaga attgcgttta taaaattatg
     1681 ggtagcgccc acaaatccaa ggctccagtc ggatggaaca ttgtagtttt tccatccctt
     1741 gttagtaaaa actccaacaa ccgggccttt attaatactc cccgtacatc gacgtataac
     1801 tactatacca tgagtgccac ttacctctat ccactcatca ttggcataat atttacaggg
     1861 gatggtaagc gaaggcaaat gaacaaactc aattgagccg taacatccat tgtcatactt
     1921 ccacatcatg actgcaggag catcaataat gccatcaatt gagtcaatcc atcctgctgt
     1981 cttttcaaca ccgccgcata aataccatgc tgttgtccat aggtgatgtc catgatcaaa
     2041 tgtatcaagc ccgcgcatgc caccgctctc ggtattttcc ttaatccgcc actgccatgc
     2101 ttctggcggc acatgccacc cgccgctgcc tcctccaata aattttatgc gcagggcaat
     2161 aggttttcca atttcgccgt tatctataat cttttttgcc agcctgatag gcgggtaaaa
     2221 tggatagtta tcggtaacct taaaaatttt accggcagtg tctgtcgcag caattatgcg
     2281 gtcggaactt ttcaggtcaa ccgtcatggg tttttgcagt gcaatatgtt ttccggcatg
     2341 agcggcagct accgtcattg attcatggag cgagtgaggg gttagaattt caacggcatc
     2401 aacatgtgca tcagctacaa gatcacggta atcggtatat actttttcgg cattccattg
     2461 tttttttctt ttctgtgcaa gttcaaggtt actatcgcac accgcatata cctttgcctg
     2521 tttattattg gtatatccca gataatgaag atcggcaatg cgtcccagcc caataaaacc
     2581 tatctgtata gatttcatga cacctccatt tattattgca tattataaaa tagtattgca
     2641 taaatgtcaa tgattttttg tgatacccgg caaacaatcc agatttacta tacacttatg
     2701 atatatgcac aacctgtgaa ttcaagtcaa ataacacagg aactatcacc aatcc
//
LOCUS       DAOSOX010000269         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36788_length_2752_cov_5.474231, whole genome shotgun sequence.
ACCESSION   DAOSOX010000269 DAOSOX010000000
VERSION     DAOSOX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36788_length_2752_cov_5.474231"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLX22_12235"
     CDS             complement(<1..879)
                     /locus_tag="PLX22_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT20707.1"
                     /translation="MAKLNLPMRDSPYIKSGLMQQYLQLLYVKLGIGFVFLLCLGAAF
                     LFSLTAGSLHIPFGKIITTLFWQNDLSYVIYNIRLPRSVAAVIAGASLGVSGCVIQNI
                     LKNPLASPFTLGISHGAAFGASVAIIGLGAGITHSYGNESVSVFNVYMVVFAAFAGAI
                     VAVSIILLLSLVKKVSPEAIVLAGVAMSSLFGASTMALQYFATDFQVSATIFWTFGDI
                     GKAGWVENLIMGICFLACFVYFYVQRWNYNALLWGDDVAKGLGVNSASLRLMGMLLCA
                     LIVSLCTAFLGIIGFVG"
     gene            complement(836..1858)
                     /locus_tag="PLX22_12240"
     CDS             complement(836..1858)
                     /locus_tag="PLX22_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT20708.1"
                     /translation="MITDFAGRRVAIPEEIKGVVALSASLRYVVYLQAFDKVVGVEEL
                     EKRNSMRTINADGRMYWAAIADRVDGIPGIGEGGPGKLPDFERLLMVKPDIVFTFEAN
                     NAEIIQGKTGIPCIVISGATGMDGFSIDDIKKTFLFMGNILGKQVRALQLCTYIDTCI
                     ADLQQRVHNTKKVSVYVGGVSARGAHGITSTESFYPPLQWLNVLNVADELGKKGHLFI
                     DMEKLIVWNCDNLFIDAAGLALIEIDYKKNSGFYKKLKAVVKNNVYTVYPYNFYRTNI
                     EVMIANSYFIGKTLYPERFNDIDTEEKARVIVKEFVGMDVYDNLKKSYKGYGKVKFTN
                     AGLTVH"
     gene            complement(1981..2385)
                     /gene="tsaA"
                     /locus_tag="PLX22_12245"
     CDS             complement(1981..2385)
                     /gene="tsaA"
                     /locus_tag="PLX22_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOT20709.1"
                     /translation="MESISYPIKPIGYIRHRHKDVPRHWSLSELEGEIVIDEDYALGI
                     HDIKAGNLIVVLFLFDRAVSFTMDRLIVKPPHCDEYLGVFSTCSPVRPNPIGLSIVEV
                     LEVHGFTIKVKRIDMFDGTPVLDIKPYIPYMS"
     gene            complement(2401..>2752)
                     /locus_tag="PLX22_12250"
     CDS             complement(2401..>2752)
                     /locus_tag="PLX22_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20710.1"
                     /translation="VLIVDADESNTGLHRMFGFSKMPQSFMEYLGGKKVVQQTMLKRF
                     RNGKNEPEMSIIEKEQFDTNNIDDDYIVKEGPLSFIFIGKIYHPLEGCACPMGAVSTA
                     AQEVRAIIGNIFTH"
BASE COUNT          928 a          624 c          381 g          819 t
ORIGIN      
        1 cccaacaaat ccaatgatac ctaaaaaagc agtacagagt gaaactatca gtgcacataa
       61 taacattccc attaagcgta aggatgcact attaacaccc aaaccttttg caacatcatc
      121 gccccataac aatgcattgt aattccatcg ctggacataa aaatagacaa aacaagctaa
      181 aaagcatata cccataatca aattttccac ccatcccgct tttccaatat caccaaatgt
      241 ccagaatatt gttgccgaaa cctgaaagtc tgtagcaaag tattgcagcg ccattgttga
      301 agcaccaaaa agactgctca tagcaacacc cgccaataca attgcttccg gtgatacctt
      361 tttaaccaat gacaaaagaa gtattattga tacagctaca atagcacctg caaatgcagc
      421 aaacaccacc atatagacat tgaaaacact cacagattca ttgccatatg aatgtgtaat
      481 ccccgcaccc aaaccaatga ttgcaactga cgcaccaaat gctgcaccat gagatatacc
      541 cagtgtaaag ggagatgcca atggattttt taaaatattc tggataacac atccagaaac
      601 gcctaacgat gctcctgcaa ttacagcagc aacagatcta ggtaatcgaa tattgtatat
      661 cacatacgaa agatcatttt gccaaaataa tgttgtgata atcttaccaa acggtatatg
      721 aagcgaacca gcagtaagag aaaacaaaaa agcagcaccc agacaaagca gaaacacaaa
      781 ccctatcccc aattttacat ataataactg taaatattgt tgcattaatc cagatttaat
      841 gtacggtgag tcccgcattg gtaaatttaa ctttgccata tcctttataa ctctttttta
      901 aattatcgta tacatccata ccaacaaatt ctttcacaat taccctggcc ttttcttcag
      961 tatcaatatc attaaaccgt tcaggataaa gcgttttccc aataaaatat gaattagcaa
     1021 tcataacttc tatatttgtt ctataaaaat tatagggata tacagtatat acattatttt
     1081 taacaactgc ttttaatttt ttataaaaac cactgttttt cttgtaatca atttcaatga
     1141 gtgcaagtcc agcagcatca ataaataaat tatcgcaatt ccacacaata agcttttcca
     1201 tatcaataaa gagatgtccc tttttgccca attcatcggc aacattcaaa acgtttagcc
     1261 attgtaaagg tggataaaaa ctttcagtgc tggttattcc atgtgcacca cgtgcactca
     1321 cccctcctac atatactgat actttttttg tattatggac cctttgctgt aaatcagcaa
     1381 tgcaggtatc aatatatgta catagttgca acgctcgtac ttgtttacct aaaatatttc
     1441 ccataaataa aaatgttttc tttatatcat caatagaaaa accatccatt cccgttgcgc
     1501 ccgatatgac aatacatgga atacctgttt taccctgtat tatttcagca ttgtttgctt
     1561 caaaagtaaa tactatatca ggtttaacca tcagaagcct ttcaaaatcc ggaagttttc
     1621 ctggaccgcc ttcgcctatg cctggaatac cgtcaactct gtctgctatt gcagcccaat
     1681 acattctgcc atctgcatta atagtgcgca tagagtttct tttctcaagc tcctcaaccc
     1741 caactacttt atcaaaagcc tgtaaataga ccacatatcg taaagaagca ctcaatgcca
     1801 ctaccccttt aatttcttct ggaatagcta ccctcctgcc tgcaaaatca gttatcacac
     1861 tattaccttt aggtaacctg gtatcacaca atatacaaaa taagcataga acgcaacagc
     1921 caattgtttt atatagatcc gtattcatag tatataaaag caactcccct gcttattggt
     1981 ttatgacata taaggaatat acggtttaat atcaagtacc ggcgtaccat caaacatatc
     2041 tatccgttta acttttattg taaaaccatg tacttctaaa acttcaacaa tggataatcc
     2101 aataggattt ggcctaactg gcgaacatgt actgaaaaca ccaaggtact catcacaatg
     2161 tggcggcttt acaatgagcc tgtccatggt aaaggataca gctctgtcaa aaagaaataa
     2221 taccacaatg aggttgcctg cctttatatc gtgtataccc agcgcataat cctcatcaat
     2281 aactatctca ccttcaagct ctgataatga ccaatggcgc ggaacatctt tatgacggtg
     2341 tctaatatac ccaatgggtt taattggata gcttattgat tccatttcac ctcttataat
     2401 ttaatgagta aaaatatttc ctattatagc acgtacttct tgcgcagccg tactaactgc
     2461 acccatcggg caagcgcacc cttccagagg atgatatatt tttccaataa aaataaaaga
     2521 taatggaccc tcttttacaa tatagtcatc atctatattg ttagtatcaa actgttcttt
     2581 ttcaataatg ctcatttcag gctcattttt gccattccta aatcttttga gcatcgtctg
     2641 ctgtactact tttttcccac ctaagtattc catgaaagat tgtggcattt ttgaaaaacc
     2701 aaacattcga tgcagtcctg tattagattc atcagcatcg actataagaa ca
//
LOCUS       DAOSOX010000270         2749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36841_length_2749_cov_6.020045, whole genome shotgun sequence.
ACCESSION   DAOSOX010000270 DAOSOX010000000
VERSION     DAOSOX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36841_length_2749_cov_6.020045"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..1844
                     /locus_tag="PLX22_12255"
     CDS             357..1844
                     /locus_tag="PLX22_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20711.1"
                     /translation="MKQCVLYKLKDFSYMNTVHYLNNSMKLALIHRKTINTMTAVVSV
                     MGGKLQAYKMIVIFFISIHLILQLLSPLHAQQVYSASTLHNAASRSQTIQHIFYQRWD
                     NMQNNFCYFDAYVNVNWVFDFDVNAYNRGTGETEAVPMKLVRTFSSMLLALPVYTPRD
                     VTTNDYQQSPQTDITNATGALPIKKSWKKNSVLLGLSVCGYHYGLTRTVEISRGSAGS
                     EKASDYVFSQFFDDIAALTLFIVPYITIHCGIIFNQQIEPNDDGTLDYNFDPSKTAKR
                     LLTSLSLFNAITWESTAASNQLEATDISVNITQFISIVKTTPQYMPAIIIGYKQFKKY
                     NDEDYDPVWVKNEKLLGDSSRDSATLYSYYATVRKNIDNFYMAASIQLQHVDQTLIDK
                     RTLETIDIPVLQMWSMKAGYDFLHKPGWDFFVWLGITHWYDPAISFHSNHNNNNVTGW
                     FAMMDIASPLFTFTLKGSYNESYELEKLVESVDKYIIEGSASFRL"
     gene            1846..2355
                     /locus_tag="PLX22_12260"
     CDS             1846..2355
                     /locus_tag="PLX22_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20712.1"
                     /translation="MQSILSKIIVVLFLVSCIGCSYIADSIEAELTNRANFSIDAYYD
                     SGSVMVSWDTNPDYDSFAGYEIYMTRDANDEFSEMIVVGAYYDIGNSSYFQQDATLGN
                     PSTSHFIHSNLPSPGIYFYRVGVIEWDERDYNNDGEDEKKPDFPDESLYKSYTHIEKI
                     SGSAMVEIF"
BASE COUNT          871 a          514 c          492 g          872 t
ORIGIN      
        1 taaaagcttt gccgaaaagt aaaacggcat atgttttttg taaagtattt tatttcataa
       61 aattgtgaaa gtggcaatta actattattt ttttatcttt ttattctatt gtagtagagt
      121 cgccaacctg ctttgttaca aaagaaatat cttgaaattt aaaaaaagtt tttattttga
      181 tatttcgaaa atgatatatc aatattttac ttgcaataac ttatttcata taacctataa
      241 tgatagttta cacaatagcc acattgcaat gacaaggagg agatgaaaat tgggaggttt
      301 ctgaacgagt aaggttttta ttttaagcga gagtacttct tcaactgctc tttcagatga
      361 agcaatgcgt tctctataaa ttgaaagatt ttagttacat gaatacagtg cactatctca
      421 ataatagtat gaagttagct ttgatacatc gtaaaacaat caacactatg actgcggtag
      481 tttctgtcat ggggggcaaa ctgcaagctt ataaaatgat agttattttt tttataagca
      541 tccatctcat tttacagtta ctatcgcccc tgcatgccca gcaggtatac agtgcttcta
      601 cattgcataa tgctgcatcc cgcagccaga caatacagca catcttctat caacgctggg
      661 acaatatgca aaataatttt tgctactttg atgcgtatgt caatgttaac tgggtttttg
      721 attttgatgt taacgcatat aatcgcggca ccggtgaaac cgaagcggtc cccatgaaac
      781 ttgtccgaac cttttccagc atgttgctgg cattaccagt gtatacaccc cgcgatgtta
      841 caaccaacga ttaccagcaa tcaccacaaa ctgatataac caatgcaact ggagctttac
      901 ccattaaaaa atcctggaaa aaaaattcag ttttactggg actttctgta tgtggctatc
      961 actatggatt aacacgaacc gttgaaatta gtcgtggaag tgcaggcagc gaaaaagctt
     1021 ctgattatgt cttttcacaa ttctttgacg acattgctgc cctgacgctt ttcatagtac
     1081 cctatattac catccattgc gggattatct tcaatcagca aattgaaccc aatgatgatg
     1141 gcacgctgga ttacaacttt gatccatcaa agactgcaaa acgccttctt accagtttat
     1201 ccctttttaa tgcaataaca tgggaatcaa cagcagccag caatcaatta gaagcaaccg
     1261 atatcagtgt aaacattact caatttattt ccattgtaaa aacaacacca cagtacatgc
     1321 ctgctatcat catagggtat aaacaattta aaaagtataa tgacgaggac tatgatccgg
     1381 tctgggtaaa aaatgaaaaa ttgttgggcg atagttcccg tgacagtgca acgctgtatt
     1441 catactatgc cactgtcagg aaaaatattg acaactttta tatggcagca agcattcagc
     1501 tacagcatgt tgaccagacc ctcattgata agcgaacact ggaaacaata gacataccag
     1561 tactacaaat gtggtccatg aaagcaggct atgatttttt acataaaccc ggatgggatt
     1621 tctttgtatg gttgggcatt acgcactggt atgatcctgc tattagcttc cacagcaacc
     1681 ataataacaa caacgtgaca ggctggtttg ccatgatgga tattgcgtca ccgcttttta
     1741 cattcactct taaaggtagc tataacgaaa gctacgaact tgaaaaactg gtagagtcag
     1801 ttgataaata tatcattgag gggagcgctt cattcaggtt ataatatgca atccatacta
     1861 tcaaaaataa tagtagtatt gtttttagta agctgcattg gttgctcgta tatagcagat
     1921 tcaatagaag ctgagctaac aaaccgcgca aatttttcca tcgatgccta ttatgacagc
     1981 ggttcagtca tggtatcatg ggatactaat cctgattacg attcatttgc aggctatgag
     2041 atatatatga ccagagatgc aaatgatgag ttttctgaaa tgattgtagt tggtgcttac
     2101 tatgatattg gcaattcatc atattttcaa caggatgcaa cacttggtaa tccatcaact
     2161 tcacatttta tacactccaa tctcccatca cctggcattt acttttaccg tgttggcgta
     2221 attgaatggg atgagcgtga ctacaacaac gacggtgaag atgaaaaaaa acctgatttt
     2281 cctgatgaat cattgtataa atcatataca cacattgaaa aaataagcgg cagtgcaatg
     2341 gttgaaatat tttaatcata gccatggata atatcttcaa taatagatgt tgttgacctc
     2401 acccccgacc ctctccttct caaggcgagg gggcattgtt tccttttgta taatttccat
     2461 taacaatggg cagcaatatg ccaggcatcg agtactcgtg caatagtttt tgctttggta
     2521 tatgattttg ttttttatat tgtgaaatca attgacccat gccatgatca ttttttggtt
     2581 gaaaatatta cacaatattc tatttttgca ctatagtatt gtttttctga taaaaagatg
     2641 tattgtttga tgttcattat ttggcaggga tagaatcctt tgaaaaccat cttcgaatat
     2701 tatttacaat gtcacctata gttacgaaag gggtaaacca tgcactact
//
LOCUS       DAOSOX010000271         2741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_36987_length_2741_cov_8.731943, whole genome shotgun sequence.
ACCESSION   DAOSOX010000271 DAOSOX010000000
VERSION     DAOSOX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36987_length_2741_cov_8.731943"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLX22_12265"
     CDS             complement(<1..392)
                     /locus_tag="PLX22_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia permease"
                     /protein_id="HOT20713.1"
                     /translation="MMCAGFSFAQDTELATVQTASDSLALNNVALDTLWVLFAAFLVF
                     FMNLGFAMVEAGLQQAKNSVNISAKNFIVFAISSIAFWFIGWGLMFGDGNGFMGYKGM
                     FMMSGPDNSPAIGNSYHGVYSALNWTGVP"
     gene            complement(450..650)
                     /locus_tag="PLX22_12270"
                     /pseudo
     CDS             complement(450..650)
                     /locus_tag="PLX22_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
     gene            839..1276
                     /gene="mraZ"
                     /locus_tag="PLX22_12275"
     CDS             839..1276
                     /gene="mraZ"
                     /locus_tag="PLX22_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT20714.1"
                     /translation="MFMGEYSPALDEKCRIAIPMKLRKAFGEENIIQKLIITHGFDKC
                     VMAFREEDWKEFVETKLVPLSQADPMNRMRIRFLLGGATECELDKQGRILIPNYLLEY
                     AQINREVVILGLYNRIEIWSAEVYKNYKPDGNALNQFAHDLGF"
     gene            1306..>1533
                     /gene="mraW"
                     /locus_tag="PLX22_12280"
     CDS             1306..>1533
                     /gene="mraW"
                     /locus_tag="PLX22_12280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOT20715.1"
                     /translation="MEYQYYHTPVMQKEVLSFLAQIQDIESATIVDCTVGEGGHSELI
                     LKQWGGVTLYGFERDKEIFAIAHKRLQSYEKV"
     assembly_gap    1534..1633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1786..>2741
                     /locus_tag="PLX22_12285"
     CDS             1786..>2741
                     /locus_tag="PLX22_12285"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOT20716.1"
                     /translation="MEIKIYNSFDLYTLLDSIDVSIIKGDEDIRISSVEYDSRRVEKE
                     SIFVAIEGFETDGHKFIKDALENKASCIVLNENRLDEFSFLLDRNDLAVVTAKDTRKA
                     LSQISARFYGEPSLQAVVIGVTGTNGKTTTTYMLESILSKAGFHCGVIGTINYRWKDK
                     KINALHTTPESKDLHEMMSIMVLDGVDCIIMEVSSHALSLLRVDDIHFDCALFTNLTR
                     DHLDYHITFNDYFMAKVRLFEILNKSCKQKKAAFINSDDNYGRQILNWRQRFNYPLFS
                     YGIQNNADYHCIESSIQTSIRGTRFATDVPFAKQFEMRLAGK"
BASE COUNT          833 a          448 c          568 g          792 t
ORIGIN      
        1 ggaactcccg tccagttgag agcagaatac actccatggt aactattgcc gatagctgga
       61 ctgttatcag gcccgctcat catgaacatc cccttgtatc ccataaagcc gttaccatct
      121 ccaaacataa gcccccatcc aatgaaccag aatgcaatgg aagaaatggc aaatactata
      181 aagttttttg cagaaatatt tactgaattt tttgcctgtt gcaatccagc ttctaccatg
      241 gcaaaaccta aattcataaa gaaaaccaga aatgcagcaa agagtaccca gagcgtatcc
      301 agtgcaacat tatttaaagc aagagagtca gaagctgttt gaactgtcgc taactcggta
      361 tcctgcgcaa atgaaaaacc tgcgcacatc atgacaataa taaaaacagt cagtattttc
      421 agaaccttca tattgtcctc ctttcaatgt tagcctatag ctcttgaacc gcgttcaccg
      481 gtttgtatgc gaatacattc ctcaatggga atgacaaaaa ttttaccatc accaatattg
      541 cctgttttgg caccgcgggt tattgcttta attgttggtt caacatagtc atcgtttaca
      601 gcaatctgga gctcaagtaa cctcgcaaag tttttcaggt caatgtttga ttgttttata
      661 tgctataaag tctttgtatt taacattgag ttaatgcatt tgtaacgtaa ttgaaacaat
      721 tgggaaattg gaatattttt gttttttact tgacaattat gtcacattgt gtacataagt
      781 ggtaaaaagt ggggattggt gttctggagt agaaagaagg gggaaagtat agaaatttat
      841 gtttatgggt gaatatagcc cagcgcttga tgaaaaatgc cgtattgcaa tacccatgaa
      901 gttacgaaaa gcgtttggcg aggaaaacat cattcaaaag cttattatca cgcatggttt
      961 tgataagtgt gttatggcgt tcagggaaga ggactggaag gaatttgtgg aaacaaagct
     1021 tgtccccctt tcacaggctg atcccatgaa cagaatgcgt attcgttttt tgttaggtgg
     1081 tgcaaccgaa tgtgagcttg acaaacaggg aaggatatta ataccaaact atctgctgga
     1141 atatgcacag ataaacaggg aagttgttat tctgggattg tataatcgta ttgagatatg
     1201 gagtgctgaa gtttacaaaa actataaacc tgatggcaat gcacttaatc agtttgctca
     1261 tgatctgggg ttttgataaa ctaatgatga ttaatgtgac ataatatgga ataccagtac
     1321 tatcatacac ctgttatgca gaaagaagtg ttgagctttc ttgctcaaat acaggatata
     1381 gaatcggcaa ctattgttga ttgtacggtt ggcgagggtg ggcattcaga attgatacta
     1441 aagcaatggg gtggcgttac gctatacgga tttgaaaggg ataaagaaat ttttgcgata
     1501 gctcataaac gattgcaatc ctatgaaaag gttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnngagtatg atcgtcaaaa gtatatatta caaaaagctg tgtcaatgaa
     1681 aaatcttgat gcctttgcac agttacatgg attggtgcca gttagtaagg aaaatgtagt
     1741 gatagttgaa agtaatgtta aaaacactat gcgggaacag agataatgga gataaagata
     1801 tacaattcat ttgatttata tacgttactt gattcgattg atgttagcat cattaaaggt
     1861 gatgaagata ttcgtatcag ttcagttgaa tatgattcgc gcagagttga aaaagaatcg
     1921 atatttgttg cgatagaagg gtttgaaact gacggacata agtttattaa agacgctctg
     1981 gaaaacaaag catcatgcat agtgcttaat gaaaacaggc tggatgaatt ttccttttta
     2041 cttgatcgta atgatctggc agtagttacc gcaaaggata cccgcaaggc tctttcgcag
     2101 atatctgcgc gtttttatgg ggagccttct ctgcaggcag tggtaatagg tgttaccggt
     2161 accaatggta aaacaactac aacatacatg ctggagtcta ttttatcgaa agcaggattt
     2221 cattgcggtg tgataggcac tattaactat cgctggaagg ataaaaaaat caatgcatta
     2281 cacaccacgc ccgaatcaaa agaccttcac gagatgatgt caataatggt gctggatgga
     2341 gtggattgta ttatcatgga ggtttcatct catgcgcttt cattgttgcg tgttgatgat
     2401 attcattttg attgtgctct atttaccaat ttaacccgtg accatcttga ttatcatata
     2461 acatttaatg actatttcat ggcaaaggtt cgattatttg aaattttaaa taaaagttgt
     2521 aaacaaaaaa aagcagcgtt tatcaatagc gatgacaatt acgggagaca gatcctgaac
     2581 tggcggcagc gctttaacta tcctcttttt tcgtatggga ttcagaacaa cgctgactat
     2641 cattgtattg agtcgtccat ccagacatcc atcaggggta cgcggtttgc caccgatgtg
     2701 ccttttgcaa aacaatttga gatgcgtctt gccgggaaat t
//
LOCUS       DAOSOX010000272         2701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_37726_length_2701_cov_7.903628, whole genome shotgun sequence.
ACCESSION   DAOSOX010000272 DAOSOX010000000
VERSION     DAOSOX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37726_length_2701_cov_7.903628"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..496
                     /locus_tag="PLX22_12290"
     CDS             371..496
                     /locus_tag="PLX22_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20717.1"
                     /translation="MRAHDRAACRRQPLHDIVDKAGEEARCAPAVERIYPEGAFL"
     gene            1100..2032
                     /locus_tag="PLX22_12295"
     CDS             1100..2032
                     /locus_tag="PLX22_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT20718.1"
                     /translation="MNDTKLWMALSRVQGVGIVALKQIYTALSNLNISLYDIVELSPK
                     TMAFETGLPIELCQLVANVSSTIGTAQKEYEQLLDNTIEPILFFDVRYPQRLLRSMEY
                     PPAILYMYGNYDVITSKQIAILSDHTASERGHYIAYQAAHIAAQHSITVTSGMAKGPQ
                     SMAHRGAIEGGGNTIAVLPMGLLHLSIPQILQDCVDLSRWLFISPFEPAQEYAIHHSY
                     KRNSLIAAMANALIIIETTPDGGSFEAAKSAHNYKTPLFVVEYAEYPATASGNPGIIK
                     EYNATPLKGRMQNGTLLPNMDMCIAKARYGDSHS"
     gene            2190..>2701
                     /locus_tag="PLX22_12300"
     CDS             2190..>2701
                     /locus_tag="PLX22_12300"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT20719.1"
                     /translation="MKYYESLEKFKTYNIVLLCGLYGAGKTEFASTYFKGSGLYRVSR
                     LELRKLMYEMTHFGESWEAEKFNEEDDVLAKHVERKVVEHFLQIKRNVLIINTFMTKK
                     SRQRFVNIAKETKKTIGAIFLDTPLDVCIAQSKRLNTAIPENVIRQLHSRKELPTKLE
                     GYNDVLIIND"
BASE COUNT          824 a          579 c          568 g          730 t
ORIGIN      
        1 catggatgca ggtgcaccct ctaccgttat accggatata tatatcttct tcttttcctg
       61 ctgcgcctgc gttgctgttt gtggtatgct ccctgctacc atcaatatac ccagcagagc
      121 taccagtatg gttttctttc ccataaaacg ttcccccaga ttggataaat aattattatt
      181 catagctgtt ttataccgtc tatgcactgt ttgcaataaa aaaatagttt tacccacttt
      241 aaacaagggg ggggatcgtc tgtcatcctg agcgcaggcg cagaatccca taattgtata
      301 aacaacaata attcaccact gttgtcattc taacaataag cagccgtgca cggatgcacg
      361 gcgcacctgg atgcgggcgc atgatagggc agcctgccgc agacaaccat tacacgacat
      421 cgttgacaag gcaggcgagg aagcccgttg tgcgcccgcc gtggaacgga tatatccaga
      481 aggcgctttt ctttgatact ttcttttgtt gcctcacaaa agaaagtatg agaagttatc
      541 aaggcttttt acacaattcc caccttttat aaagggggga acacaaggga aaaatgaaaa
      601 agtagcggag gcacggatgc cgtagcgctc tacggctcgt gcgaggacat agacaggatg
      661 tcttccgcag cacgatggca aagttatatt attatacaga aaataaggct caccgccgtt
      721 tgaggtgcgc tttgggggtt ggtaaggggg tatttagcgc ataaatatcc ccttacgaaa
      781 tgattaaact tttgtcgccc cacaaaagaa agtatgaata gttatcaagg ctctttacac
      841 cattcccacc ttttataaag gggggaacac aaggggggtt atgtcatccg tcattctgaa
      901 cgaagtgaag aatctcgctg tttgatgaac aaatagattc ttcagtcgct ggcgctttct
      961 cagaatgaca atggtgcgta gacgttgccc atggccagct tataacgtat tgaaataaga
     1021 aaataaaaaa gtgttgattt tatagcttgt tttaccatga gtaaattcat tttataataa
     1081 taaactcatc gacaatacta tgaatgatac aaaattatgg atggcactgt ctcgtgttca
     1141 aggcgtgggt attgtggcgc tgaaacaaat ttatactgca ttgtccaatc tcaatatttc
     1201 tctgtatgat atcgtagaac tatcgccaaa aacgatggct tttgaaacag gacttcccat
     1261 tgaattatgc cagcttgttg ccaatgtatc atcaactatc ggcacagcac aaaaagaata
     1321 cgagcaattg cttgataaca ctattgaacc cattttattt tttgatgtac gctacccgca
     1381 gcgtttgttg cgttcaatgg aatatcctcc tgccatactg tatatgtatg ggaattacga
     1441 tgtaatcaca tcaaaacaga tagcaatact atccgaccac actgcaagcg agcgtggtca
     1501 ctacattgcc taccaggcag cacacattgc agcacaacat tccatcacgg taaccagtgg
     1561 catggcaaaa ggtccgcaaa gcatggcaca ccgcggtgca attgaaggcg gcggcaacac
     1621 catcgctgtg cttcccatgg gattgcttca tctgtcaatc ccgcagatat tacaggactg
     1681 tgtggacctt tcccgctggc tattcatatc gccatttgaa ccagctcagg aatatgccat
     1741 tcaccattcg tataagcgca atagcctcat tgctgccatg gccaatgccc tgataatcat
     1801 tgaaaccaca ccagacggcg gtagttttga ggcagcaaaa agcgcacata actataaaac
     1861 cccgttgttt gttgttgaat atgctgagta ccccgctacc gcatcgggca accccggtat
     1921 catcaaagaa tacaatgcca caccgcttaa agggagaatg caaaacggca ccctgctacc
     1981 caacatggat atgtgcattg caaaggctcg ctatggcgat agtcactcat aatttaatta
     2041 taagcatcat ataaccgata ctgattatag tgttaccgta taatagctgc ttatatgata
     2101 gcaaattgtg attgatttat tcacaagttg atgattgcat tgagctatcg caccaccatt
     2161 actgtaaaca ataaacaggg gtattataaa tgaaatatta tgaatcgtta gaaaaattta
     2221 agacatacaa cattgtttta ttatgcgggc tttatggtgc aggtaaaact gaatttgcca
     2281 gcacctattt taagggttcg gggctatacc gcgtgtcacg ccttgagctg cgcaagctaa
     2341 tgtacgagat gactcatttt ggtgaaagct gggaggctga aaaatttaat gaagaagatg
     2401 atgtgctggc aaaacatgta gaaagaaagg ttgtagaaca ttttttgcag attaaacgca
     2461 atgtacttat catcaacacc tttatgacaa agaaatcccg tcagcgtttt gtaaacatag
     2521 caaaagaaac taaaaagacc atcggagcaa tatttttaga cactcctctt gatgtatgta
     2581 ttgcacagag caagcgtttg aatacagcaa ttcctgaaaa tgtcatccga caactgcaca
     2641 gcagaaagga actgccaaca aaattggaag ggtataacga cgttttaata atcaatgatt
     2701 t
//
LOCUS       DAOSOX010000273         2676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38162_length_2676_cov_8.640977, whole genome shotgun sequence.
ACCESSION   DAOSOX010000273 DAOSOX010000000
VERSION     DAOSOX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38162_length_2676_cov_8.640977"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /locus_tag="PLX22_12305"
     CDS             <1..2104
                     /locus_tag="PLX22_12305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOT20720.1"
                     /translation="YTIADQSFKTASRYISAFVDLLDALTGRMEADIVMELLDYDEIA
                     TVFAISKQDVAVYQDWVSRVNVRWGKDAKHREQFIQKESKAFTWQYAMERFLMGYAVP
                     ENTGIIDDIVPFDCVEGTAAQRLGNFIMFLKTLFSFHTIAGQKHSISEWAARLEQLMS
                     SLLVNNDTVSNDIVFVQSLLQQLVATSATLQITDEIDFIVIKDWINTYLAGQRVSTNF
                     LRKGITFCQLLPMRSIPFQVVCLLGMNDGQFPRIDERLSFDILKNKEKQPRCVRSKRN
                     DDRYLFLESMLSAQKKLFISYEGQSPVDLSLKQPSLVVAELMDYIEQGFQCSDTAQSI
                     SNFILTKHHLHRFHNNYFNNDSKKYSYSKLSYHESKAWYGSKHRYHLLVQTPVAVDQM
                     PFHITMDDFISFFKNPARYFIKNVLGISLAENVNDIASDEPFSINGLEHYQLTNELCQ
                     RLINGGDSYGYKVLLKKQGKLPDGDLGDIIFEDAQSQVMDFVAKVKSKITCDAEQHEL
                     HYTSNNNLVITGNPYLYANQQIYYRYATIKPDDRLHALLWHLFVCATQSDECNDKFTT
                     LLIGKDNDKNKYKELTYQHLLKEKSINDLEQLVSIFLQGQSTILPFFPKTSYVYYNAV
                     TDGTSSKSKNALYKARDAFYGDNGYESIDYDNPYIKRAFYKVDIFDDEKLYNDFDNLS
                     HTIFGIVKDYESEEKESQ"
     gene            2101..>2676
                     /locus_tag="PLX22_12310"
     CDS             2101..>2676
                     /locus_tag="PLX22_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT20721.1"
                     /translation="MNTIAIPEKFTDYALDGINCIEASAGTGKTYTITHLFLRLIIEN
                     DLPLDAILVVTFTNAATDELRERIRRLLKTAYDVVDSNFDDAVINGYKKNDELISYLK
                     KLCKEHDSEDYRLQIALRLQLALHDIDNAHVYTIHSFCRQALSEFAFESGSLFGSEVV
                     TDASPYYRRVIEEFFYKEIAPLGKEFMYYFIS"
BASE COUNT          868 a          469 c          544 g          795 t
ORIGIN      
        1 atacactatt gccgaccagt catttaaaac agcaagccga tacatctcag catttgttga
       61 tttgcttgat gcgttaacgg gaaggatgga ggcagatatt gttatggaac ttctggacta
      121 tgacgagata gcaacggtgt ttgctataag caaacaggat gtcgcagtat atcaggattg
      181 ggtttcgcgt gtaaatgtac ggtgggggaa agatgcaaag catagagaac agtttataca
      241 aaaagaaagc aaagccttca catggcaata tgcaatggag cgcttcttga tgggatatgc
      301 agtcccggaa aatacaggaa ttattgatga tattgtgccg tttgattgtg ttgagggaac
      361 agcagctcaa cgattgggta attttatcat gtttctgaaa acactatttt cttttcacac
      421 aattgccggt caaaagcata gcataagcga atgggcagca cgattagagc agctaatgtc
      481 ttcgctgctt gtcaataacg ataccgttag caatgatatt gtttttgttc aatcgttgct
      541 gcagcagctt gtagcaactt ctgcaacatt acagataacc gatgaaattg attttattgt
      601 tataaaggat tggatcaata cctatttagc cgggcagcgc gttagcacca attttttacg
      661 taaaggtatt accttttgtc agcttcttcc catgcgaagt atcccctttc aggtggtttg
      721 tcttttagga atgaatgatg gtcagttccc acgcattgat gaacgcttaa gttttgatat
      781 cctgaaaaac aaagaaaagc agccgcgctg tgttcgttca aagcgcaatg atgatcgcta
      841 tctttttctg gagagcatgc tatcggcgca gaagaaattg tttataagct atgaaggtca
      901 aagccctgtt gatttaagcc tgaaacaacc ttcactggtt gtagctgaac ttatggatta
      961 tattgagcag ggttttcaat gcagcgatac cgctcaatca atcagcaatt tcatactaac
     1021 aaagcatcac ctgcacaggt ttcataataa ctattttaac aatgacagca aaaaatattc
     1081 atattcaaaa ctatcgtacc atgaaagtaa ggcatggtat ggtagcaagc ataggtatca
     1141 cctattggta caaactccag tggctgtgga tcaaatgccc tttcatatta ccatggatga
     1201 ttttatatct ttttttaaaa atccagcacg ctacttcata aaaaatgtat tgggaatatc
     1261 gctggcagaa aatgtcaatg atattgcaag cgatgaacct ttttcaatca atggactgga
     1321 acactatcag ttgaccaatg agctgtgtca aaggctcatt aatggtggcg atagttatgg
     1381 ctataaggtt ttgttgaaga aacaggggaa actccctgat ggtgatttag gggacatcat
     1441 ttttgaagat gcgcagtcac aggttatgga ttttgttgca aaggtaaaga gcaagatcac
     1501 ctgcgatgca gaacaacatg agttgcacta tacaagcaat aataaccttg tgattacagg
     1561 gaatccttac ctgtacgcaa accagcagat atattaccga tatgccacaa taaaaccaga
     1621 tgacagattg catgcattgt tatggcattt atttgtatgc gcaacacaaa gcgatgaatg
     1681 taatgataaa tttaccacac tactcattgg taaggataat gataaaaata aatataaaga
     1741 gttaacctat caacatctat taaaagaaaa atcaatcaat gatcttgagc agcttgttag
     1801 tatatttttg caagggcaat caaccatact gccttttttt ccaaaaacat cgtatgtata
     1861 ttataatgca gtcaccgatg gtacaagctc taaaagcaaa aatgcactat ataaggcacg
     1921 tgatgccttt tatggtgata atggctatga aagtattgac tatgataatc cttacattaa
     1981 gagggcattc tataaggttg atatctttga tgatgaaaag ttatataatg actttgataa
     2041 tttatcacat acaatttttg ggatagtaaa agattatgaa tctgaagaaa aggaatcgca
     2101 atgaacacaa tagctattcc agaaaagttt actgattatg cacttgatgg cattaactgc
     2161 attgaagcaa gtgctggtac cggcaaaaca tataccataa cccatctttt tttgcgcctc
     2221 atcatcgaaa atgaccttcc actggatgct attcttgttg taacctttac caatgctgca
     2281 accgacgaat tgcgtgaacg gatacgccgc cttttaaaga cagcgtacga tgtagttgat
     2341 agtaattttg atgatgccgt aatcaatgga tataaaaaaa atgatgaatt aatcagctat
     2401 ctaaaaaaat tatgtaagga gcatgattca gaagactatc gcctgcaaat agcattacga
     2461 ttacaattag cattgcatga tattgataat gcacatgttt acaccattca cagcttttgc
     2521 agacaggcat tgagcgagtt tgcatttgaa agcggttccc tctttggcag tgaggtggtt
     2581 acggatgctt caccatacta ccgtcgtgtc attgaagaat ttttttataa agaaattgca
     2641 ccactgggca aggaattcat gtattatttt ataagc
//
LOCUS       DAOSOX010000274         2673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38220_length_2673_cov_5.948816, whole genome shotgun sequence.
ACCESSION   DAOSOX010000274 DAOSOX010000000
VERSION     DAOSOX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38220_length_2673_cov_5.948816"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2673
                     /locus_tag="PLX22_12315"
     CDS             <1..>2673
                     /locus_tag="PLX22_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT20722.1"
                     /translation="IIVPINYKGTVIKDNEGKVLGAIIVIQDQTKESFLQLLSEIRIR
                     FVNYAIEHTRDEFIHHALKNLADLTDSFAGCFIGYNYYFNKTNDFNQYLLPDFCNIYC
                     ADTTTEISIDYNSVWQQCFECKSVIINDSNTSYFSLNNNQNKKDKHELFIPIIDNNNT
                     VAVIGFANITREYTENEKSKIMILADILYEMIVYKEKEETYKNLVTLTNDLVCIIDLH
                     NATFILVNPSFVKILGYSEEELKSKSFVEFIHPEDVEKTLGIMNNTLLAGENIETFEN
                     RYRCIDGTYRWLDWNAHPIASMGLAFAIARDVTERKQLEDILRKSEEKFKNLFIHSND
                     GISLHQLVYDNNGNPVDYIILDVNPKYEEMLSINKNEAIGKRSTEVYKQATPPYFDIY
                     KEVTLTGNPTVFETFYEPAGKYFIISVYSPQKGQFATIFKDITPFKKSEQELQESEKK
                     FSLFMNNLPLVVVIRDIMGHYVFMNDKWEDAMDLKKEDWLGKTPFDVFPEEDAKKLIQ
                     IDHDAIVNGTTEPIELTLHHKTGIKHWLANRFVIYNTNNKPVYVATLYIDISEKQKVE
                     EERERLKDQLIQAQKLESIGRLAGGVAHDYNNMLEVIMGNAQLAMMQLENTAELKIFL
                     NEILNASRRSAEITRQLLTFARKQITEPQIVNVNFVIETMLKMLGRLIGENIELIFNP
                     ENKIWNILIDPTQINQIIANLCINARDAIDANGIITITTNNVTLDEYYCSQAPDCLPG
                     EYVMISVNDNGCGMDKEIMDKIFDPFFTTKEFGKGTGLGLSTVYGIVKQNNGFIKVYS
                     EKGSGSTFNIYFPRYVTENEYQETEIIDDKPLGNGETVLVVEDEQSLLKMIQIMLERL
                     QYNVIAVSSPLEAIELVKSRGNEIALMLTDLVMP"
BASE COUNT         1027 a          370 c          472 g          804 t
ORIGIN      
        1 gcataatagt cccaatcaat tataaaggca ctgttattaa ggataatgaa ggcaaggtgt
       61 taggagcaat tatcgttatc caggatcaaa caaaagaaag ctttttacaa cttctttcag
      121 aaataagaat acgttttgtt aactatgcca ttgaacatac cagagatgaa tttattcacc
      181 atgcattaaa aaatctggcc gatttaaccg atagttttgc aggttgtttt attggttaca
      241 actattactt taacaaaaca aatgatttta atcaatattt actgcctgat ttttgcaata
      301 tatattgtgc agatactact actgaaattt ctattgacta taacagtgta tggcaacaat
      361 gttttgaatg taaaagtgtt attattaatg attccaatac atcttatttt tctttaaaca
      421 ataatcaaaa taaaaaagat aagcatgaat tatttatccc tattattgat aataataata
      481 ctgttgcagt aattggcttt gccaatataa caagggagta tactgaaaat gaaaaaagca
      541 aaataatgat attggcagac atactatatg aaatgattgt atataaagaa aaagaagaaa
      601 catacaaaaa tcttgtcact ttaacaaatg atttagtgtg cataattgat ttacataatg
      661 ctacatttat tttagtaaat ccatcttttg taaaaatttt gggatacagc gaagaagaat
      721 taaaatcgaa atcatttgta gaatttatac atcctgaaga tgttgaaaag acattaggga
      781 ttatgaacaa tacattgctg gcgggagaaa atatagaaac atttgaaaat agatatcgtt
      841 gcatagatgg aacatacaga tggctggatt ggaatgctca tcctatagct tctatgggtt
      901 tagcgtttgc tatagcacgt gatgtaacag aaagaaaaca attagaagat atattgcgta
      961 aaagcgaaga aaaatttaaa aatttattta tccattcaaa tgacggtatt agtctacatc
     1021 agcttgttta tgataataac ggaaatccag tggattacat tattctagat gtaaatccta
     1081 aatatgagga aatgctgtca ataaataaaa acgaagctat tggcaaacgc tcaacagaag
     1141 tttataaaca ggcaacacca ccttattttg acatatacaa agaagtaaca ttaaccggta
     1201 atccaacagt ttttgaaaca ttttatgaac ctgctggtaa atattttata atttctgtgt
     1261 attcacctca gaaggggcaa tttgctacaa tatttaaaga tattacaccg tttaaaaaat
     1321 cagaacaaga gttgcaggaa tcagaaaaga aattttcgtt attcatgaat aatctaccac
     1381 ttgtagttgt tattagagac attatgggcc attacgtctt tatgaatgac aaatgggaag
     1441 atgctatgga tttaaaaaaa gaagactggc tgggcaaaac tccatttgat gtatttcctg
     1501 aagaggatgc aaaaaaattg atccagatag atcatgatgc aattgtaaac ggtacaacag
     1561 aacctattga actgacttta caccataaaa cgggtattaa acactggctg gcaaaccgtt
     1621 ttgtgattta taacactaat aataaacctg tgtatgttgc taccctgtat attgatataa
     1681 gcgaaaaaca aaaagttgaa gaggaaaggg aacggttgaa agatcaatta attcaagctc
     1741 aaaaattaga atcaatagga aggcttgctg gtggagttgc acatgattac aataacatgc
     1801 tggaagtaat tatggggaat gcacaattag caatgatgca attagaaaat acagcggaat
     1861 taaaaatatt tttaaatgaa atattaaacg catccagacg ttcagcagaa ataaccagac
     1921 agctcttaac atttgcccgt aaacaaataa cagaaccaca gatagttaat gtaaattttg
     1981 taattgaaac catgctaaag atgttgggtc gcttaattgg cgaaaacata gaactaattt
     2041 ttaatcccga aaacaaaata tggaatattt taatagatcc cacacaaata aaccagatta
     2101 ttgctaattt gtgtattaat gcccgcgatg caatagatgc taatggcata ataaccataa
     2161 caaccaataa tgttacactt gatgaatatt actgttcgca ggctcccgat tgtttaccag
     2221 gtgaatatgt aatgatatcg gtaaatgata acggctgtgg tatggataag gaaatcatgg
     2281 ataaaatatt tgatcccttc tttacaacaa aagaatttgg taaaggcact ggactgggtt
     2341 tatcaacagt atatggtata gtaaaacaaa ataatggttt tattaaagta tatagcgaaa
     2401 aaggtagtgg aagtaccttc aatatatatt tcccacgata tgtgacagaa aatgaatatc
     2461 aggaaacaga aatcattgat gataagcctt taggtaatgg tgaaacagtg cttgtggtag
     2521 aggatgaaca atcattactg aagatgatac agataatgct tgaaagatta cagtataatg
     2581 ttatagcagt tagttctcct ctagaggcta ttgaactggt aaaatcaaga ggtaatgaga
     2641 ttgcattaat gcttaccgat cttgtaatgc ccg
//
LOCUS       DAOSOX010000275         2659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38472_length_2659_cov_9.272657, whole genome shotgun sequence.
ACCESSION   DAOSOX010000275 DAOSOX010000000
VERSION     DAOSOX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38472_length_2659_cov_9.272657"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLX22_12320"
     CDS             <1..283
                     /locus_tag="PLX22_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOT20723.1"
                     /translation="VCTTLSVSPYKDAEKINEIGRSFAELYGIQFIEADFKQNGGYQH
                     SIVLSKQYGMYRQKYCGCVYSRLERNKNSLWSIKSNSYKKNMITANNKY"
     gene            626..>2659
                     /locus_tag="PLX22_12325"
     CDS             626..>2659
                     /locus_tag="PLX22_12325"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT20724.1"
                     /translation="MAKKTFEAIRKNIESNPFYAIGLGLVIGIIIIGFLFTSVYKLFE
                     YQLFDVRFKIKPQLQQWEYLSFLDIDDNSISNVGQFPWPRYVYAEGIDVIKDLGIKQV
                     AFDIQFIDKSPRYAQPDILKIVEDKIKAGKRITYDELNHAILDSDALFAGACAKNNVI
                     LSYSFLNRTLAEMNITPEERKKRDEAITRFEKIASIPVPEGSKNEYSRYIDPTRIIIQ
                     YPIPELIMNGKDFGYVDSDFDIDGIARRIRLIRVFNDRIYFHMALIMLMDILNVNKSD
                     VVVVHDQYLLLKNATDPLTLHKKDFKISIDSRGMAYIHWSGDFKDTFNHVSFYALLEY
                     NQLKDTIHEYFDEQEIQSQNTERSKLYGELAQLDELLQKEKNVIAKQKVWLKINDLRR
                     RIYTIENGYIQNLERQLQEINQQLKTSDNVQLHEAKTNLNNFINASRLVLEVDRLKGH
                     SCIIGLTATASHDIGVTPLSSEYLMVGTYHNFINTVLQNAYIKEVPRWITYVLIIIFA
                     ILIALSVQRLSAIGTINVIVGSLIAYNIVNIALFAFSYIYIDQLGASLALLLPSTVIS
                     SVKFMSEESQKRYIKNVFSKYMAPRVVDELIKNPNALQLGGELRNISIFFSDVEGFSS
                     ISEKLTPQQLVSLLNEYLSEMTDIILRYEGTVDKYEGDAIIAFFGAPLAFEDHP"
BASE COUNT          931 a          389 c          487 g          852 t
ORIGIN      
        1 agtttgtact acgctatcag taagtccata taaagatgct gaaaaaatca atgagattgg
       61 gcggtcattt gcagaattgt atggaataca atttattgaa gcggacttta agcaaaacgg
      121 cgggtatcag cattcgattg tattgtcaaa acaatatggc atgtatcggc agaaatattg
      181 tggctgtgtg tattccaggc ttgagagaaa taaaaattca ttgtggtcaa ttaaaagtaa
      241 tagttataaa aaaaatatga taactgcaaa caataagtat tgatacaggt tccatattca
      301 aaggttacta tcgtacaatt actattgtac tataagcttt tttccatgct aaccagtaat
      361 ttaaaggcta aatagtttat caccactatg tgccctccat gtaatataca tgatattgta
      421 cattggtaaa aaatactgca atataaacac tacttattgc aggatatgaa gatgggcaat
      481 gtgatattac cgtattatat ccaaaaaaga taagacgata gaaaaaaaat taaaaaatag
      541 tgtttggtat tctgcttgta aatttagaat gtatattata atgtattacc tattgatttg
      601 tacatttata taaatcgggt gatatatggc aaaaaaaact tttgaagcaa tcagaaaaaa
      661 tattgaatca aatccgttct atgcaattgg tttaggattg gtaataggaa tcattattat
      721 tggattttta tttaccagtg tatataagct atttgaatat caactgtttg atgtaaggtt
      781 taagattaaa cctcaattac agcaatggga atatctttca ttccttgata ttgatgacaa
      841 cagtataagt aatgttggac aattcccatg gccacggtat gtgtatgccg aagggattga
      901 tgtcattaaa gaccttggta ttaaacaggt ggcatttgat attcaattca tagataaatc
      961 accgcgttat gcacaacctg atatcctgaa gattgttgaa gacaaaataa aagctggcaa
     1021 acgaataacc tatgatgaat taaaccatgc aatactggat agtgatgcat tatttgccgg
     1081 ggcatgtgca aaaaataatg tcattttatc atattcgttt ttgaatagaa cgcttgctga
     1141 aatgaatata acccctgaag aaagaaagaa gcgtgatgaa gccattacac gctttgaaaa
     1201 aattgcatct atacctgttc ctgaaggaag taaaaatgaa tattcacgat atattgatcc
     1261 tactcgtatt attattcaat atccaatccc ggaattaata atgaatggga aagattttgg
     1321 ctatgtcgac agcgattttg atattgatgg catcgctcgc aggatacgcc ttatacgagt
     1381 attcaatgac agaatatatt ttcatatggc actgattatg ctcatggata tacttaatgt
     1441 taataaaagc gatgtggtag tagtccacga ccagtatctg ctattaaaga atgctaccga
     1501 tccattgact ctccataaaa aggattttaa aatttcaatc gatagcaggg gaatggcata
     1561 tatacattgg agtggtgatt ttaaggatac ctttaaccat gtatcatttt atgccttatt
     1621 ggaatataat cagcttaaag ataccatcca tgaatatttt gatgaacagg agatacaatc
     1681 acaaaataca gaacggagca agttgtatgg tgaactggca cagttagatg agttattgca
     1741 aaaagaaaaa aatgtcatag caaagcaaaa agtatggtta aaaataaatg atttgagacg
     1801 gcgtatctat accatagaaa atggctatat tcaaaatctt gaacggcaat tacaggaaat
     1861 taatcaacaa cttaaaactt ctgataatgt ccaattgcat gaggcaaaaa caaatcttaa
     1921 taattttatc aatgcatcaa ggcttgttct tgaagtggac agattaaaag gtcattcctg
     1981 tataataggt ttaaccgcta cagcctcgca tgatattggg gtaaccccgt tatcttctga
     2041 atatcttatg gtaggcacct atcataattt tattaatact gtattacaaa atgcatatat
     2101 caaagaggtg cctcgttgga ttacttatgt gctcattata atttttgcaa tacttattgc
     2161 tttatcggtg cagcgactaa gtgctatagg aaccataaat gtaatagttg gttcactgat
     2221 tgcctataac attgtcaata ttgcattatt tgcatttagc tatatataca ttgatcaatt
     2281 gggtgcttcg ttagcgttat tgttgccttc tacagttata agcagtgtaa aatttatgag
     2341 tgaagaaagc caaaaacgat atattaaaaa tgttttttca aaatatatgg cgcctcgtgt
     2401 ggttgatgaa ctgattaaaa atcccaatgc attgcaatta gggggtgaac tacgaaatat
     2461 aagcatattt ttttctgatg tggagggatt ttcatccatt tcggaaaagt taacacctca
     2521 acaacttgtt tccttactta acgaatattt atctgaaatg accgacataa tattgcggta
     2581 tgaaggtaca gtggataaat atgaaggcga tgcaattatt gcgttctttg gtgctccatt
     2641 agcttttgaa gaccatcct
//
LOCUS       DAOSOX010000276         2656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38532_length_2656_cov_8.620531, whole genome shotgun sequence.
ACCESSION   DAOSOX010000276 DAOSOX010000000
VERSION     DAOSOX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38532_length_2656_cov_8.620531"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PLX22_12330"
     CDS             <1..1455
                     /locus_tag="PLX22_12330"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOT20725.1"
                     /translation="EGVDIDINFGNPIAISPYLKSYLAKRKIKSKNLYLQPQDLQNDV
                     VLRKIAIDIMYRYMHDIYTMTTINHDHIFSYLLTRATNKIKEGDFKTKAYCAIAALKK
                     LELPSCHSALKLKQGYLLTDDPHGRYNDFIDAAKSEGLITVNNGIIYKNKQKFSRAYE
                     FHTIRKDNIVEVLKNEIEPLRNLTRRLQILYWTPMVCVRRSIAQSFYQRDVQIFENDY
                     KHYFKQGETKPKYIGMPFFKKRLFARAGVVLVHGYMAAPAEMRELADFLYNQGYTVYC
                     ARLRGHGTSPEDLATRSYDEWYESVNRGYIVVKNLVKKVFICGFSTGAGLALLHAANK
                     KDALCGAVAISAPYKLKNIASGLAPAVDTWNKFLSFLKIKKIGRLDFIPNHPDHPDIN
                     YLKNPVSGIHQMETLMDVVEKRLNDIYIPVLIMQGAGDTTVDPRGAFELFMKIPSEKK
                     ECTMVHSKSHGITRGDVGIVVARRVGAFLHDYRI"
     gene            complement(1460..>2656)
                     /locus_tag="PLX22_12335"
     CDS             complement(1460..>2656)
                     /locus_tag="PLX22_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20726.1"
                     /translation="SAQINGTAMGGIVTVTSEGSNDTTRNPALLGIVANSIINMYATG
                     TAFYDEETNPRLHASDINIQSVQQSNDYYYTFSIVAGYAKPLGKGTIGVSLSSKDNLY
                     LRRKDVQKLSGDVFGISFTQTQTTTTDEIAPTVTIAYGWKLFNNNFAGLQIAVTPFLS
                     NNDTKTDNSLSPSYSFTLREYALIIQPAIGYLITDGTSQVGLRFIPSTVKLTKKKVDA
                     NFSTGDSSYNDDLKIQQLEGPQINAGGYVQIIPTLGIALEAGFIMPSEYSNTSIQVTD
                     NPSPAIKHQSIEVKNNPILLLKGGTQYTINGSLQIMSGIAFFHLFNNAANSNSKGRGK
                     YNFLLMTLGCNYIYTPDIVITGLLIVSRNSIDTYYHLEDSISLESKTKSNVWAITVGA
                     GISYKL"
BASE COUNT          795 a          468 c          569 g          824 t
ORIGIN      
        1 gaaggtgttg atatcgatat caattttggc aatcccattg ccataagccc ctatttaaag
       61 agctatctgg caaagaggaa aatcaaaagt aaaaaccttt acctgcaacc acaggatttg
      121 caaaacgatg tagtgctacg gaaaattgcc attgatatta tgtatcgata tatgcatgac
      181 atttatacaa tgacaaccat caaccatgat catatctttt cgtatttatt gacgagagct
      241 acaaacaaaa taaaagaagg tgattttaaa acaaaggcat attgtgcaat tgcggcatta
      301 aaaaaattag aattaccatc gtgccatagc gcattaaaat tgaaacaggg ctatctttta
      361 accgatgatc ctcatgggcg atataacgat tttattgatg cggctaaatc ggaaggactt
      421 ataactgtca acaatggcat tatctataaa aataaacaaa aattttcccg ggcatatgag
      481 ttccatacaa tacggaagga caacattgtt gaggttttaa aaaatgaaat agaaccgttg
      541 cggaatctga cgcgcaggtt acaaattttg tattggaccc caatggtgtg cgtgcggcga
      601 agtattgcac aatcgtttta tcagcgcgat gtgcagatat ttgaaaatga ctataagcac
      661 tattttaaac agggcgaaac aaaaccaaag tatattggta tgccgttttt taaaaagagg
      721 ctgtttgcaa gagcaggggt ggtgctggta catggttata tggctgcacc tgcagagatg
      781 cgtgagcttg ctgatttttt atataatcag ggatacacgg tatactgtgc acggcttaga
      841 gggcatggca catcaccaga ggatcttgca acaaggagct atgacgaatg gtatgaatca
      901 gtaaaccgcg gctatattgt tgtaaaaaat ttagttaaaa aggtatttat ctgtggtttt
      961 tctaccggtg ccgggcttgc gctgctgcat gctgctaata aaaaagatgc tctatgcggc
     1021 gctgtggcaa tcagtgcacc gtataaatta aagaatattg cttcagggct tgctcctgcc
     1081 gtggatacat ggaataaatt tttatcattt ttaaaaataa aaaagattgg caggctggat
     1141 tttatcccca atcatcctga tcatcctgat attaactatc ttaaaaatcc cgtttcgggc
     1201 attcatcaga tggaaacatt gatggatgtt gttgagaaac ggcttaacga catttatatc
     1261 ccggtattaa taatgcaggg tgctggcgat acaacggttg accccagggg tgcttttgag
     1321 ctgttcatga aaataccctc agaaaaaaaa gaatgtacca tggttcactc aaaaagccat
     1381 ggtataaccc gcggtgatgt gggtattgtt gttgcacgga gggttggagc ttttttgcat
     1441 gattacagga tataacaaat tataatttat acgatatgcc tgcacctact gtaatggccc
     1501 acacattaga ttttgttttt gattccagcg aaatgctgtc ctctaaatga taatatgtat
     1561 caatgctgtt acgtgacacg ataagcaagc ctgtaattac gatatcaggc gtatagatat
     1621 agttgcatcc caatgtcatt aataaaaaat tatattttcc ccttccctta ctgttgctgt
     1681 tggcagcatt attaaaaaga tgaaaaaatg ctatgccact cataatctgc agtgatccat
     1741 tgatggtata ttgggtgccg ccttttaata gtagtattgg attgttcttc acttctatag
     1801 actgatgttt aatggcggga gatggattgt cagttacctg tatactggta ttgctgtatt
     1861 cgctgggcat aatgaaacca gcttccagtg ctattcctaa tgttggaatg atctgtacat
     1921 atccccctgc attaatctga gggccttcca attgttgaat cttcaagtca tcattatatg
     1981 aactatcgcc tgtgctaaaa ttggcatcta cttttttttt ggtcaattta actgttgaag
     2041 ggataaaacg taatccaacc tgtgatgtcc catcggttat caggtaacca attgcaggtt
     2101 gtatgataag tgcatattct cttagtgtaa agctgtatga aggggataag ctattatcgg
     2161 ttttggtgtc attatttgac aggaatgggg ttacggcaat ttgtaatcct gcaaagttat
     2221 tgttaaataa tttccatcca taggctatgg taacggtggg ggctatctca tcagttgtag
     2281 tggtttgtgt ctgggtaaaa gaaatgccga aaacatcacc actgagtttt tgtacatctt
     2341 ttcttcgcag atacagatta tcctttgatg aaaggctgac ccctattgtt cctttgccta
     2401 aaggttttgc atatccagca acaatagaaa atgtatagta gtaatcattg gattgttgaa
     2461 ctgactggat atttatatct gaagcatgca ggcggggatt agtttcttca tcatagaaag
     2521 ctgtgcctgt ggcatacata ttgataatag agttggctac gatccctaaa agggcaggat
     2581 tccgcgtggt atcattgctt ccttcggatg ttactgttac tataccgccc atggcagtgc
     2641 cattgatctg tgcact
//
LOCUS       DAOSOX010000277         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38688_length_2648_cov_9.021211, whole genome shotgun sequence.
ACCESSION   DAOSOX010000277 DAOSOX010000000
VERSION     DAOSOX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38688_length_2648_cov_9.021211"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLX22_12340"
     CDS             <1..254
                     /locus_tag="PLX22_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT20727.1"
                     /translation="VNNLQFVIIIGVLAGIGISASQIIPFSMIPDVIEYDEYSSGIRR
                     EGAFYGITTFLYKVASTAAIAGASAMLAYFGYVENSTMA"
     gene            288..410
                     /locus_tag="PLX22_12345"
     CDS             288..410
                     /locus_tag="PLX22_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20728.1"
                     /translation="MAFAPGVCFLISVCFVRMLPITRERFDEIKHLLDERKKQQ"
     gene            441..971
                     /locus_tag="PLX22_12350"
     CDS             441..971
                     /locus_tag="PLX22_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20729.1"
                     /translation="MKRLLSSGIIVMALISIVMVSCSRKEKGRFYSDQYHFSIVFPRT
                     WEIQQKEMGTVVIGLSPEEGLKDDFRENITITTEDAGDDVTLEKYFDISQKNMKNYST
                     DYKLLYKNYTWLAKKRAMILGFTYTMSGLKLKALQYYIIHNKRAYVITCTGLPHTFGR
                     YEERFLTIVRSFRLEK"
     gene            complement(974..1798)
                     /locus_tag="PLX22_12355"
     CDS             complement(974..1798)
                     /locus_tag="PLX22_12355"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOT20730.1"
                     /translation="MIISASRRTDIPAFYSQWFMDCIHRGSCTVKNPYNPNQIREISL
                     LPEDVEAIVFWTRYPAPLLQYLDELDCGGYHFIFLFTITGYPQWLEAHSPELSQSIKI
                     FKALSNKIGAQKVIWRYDPIVFSRELDFDFHINNFRAIASELHHHTQRVIVSIMEPYS
                     SVIKRITKHLIDRNIVLNPFADYNAEQMEQFFSSLSSIAHERGMHIQSCCSELSLYGI
                     SNGACIDQQLLSNIFNIQLNVPRDAHQRKNCLCTKSVDIGAYNSCRFGCMYCYASR"
     gene            complement(1817..2548)
                     /locus_tag="PLX22_12360"
     CDS             complement(1817..2548)
                     /locus_tag="PLX22_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOT20731.1"
                     /translation="MKIYKNIRIVYEDNHIIVVEKPAGILSQKDKTNEQSLLDIIKEY
                     IKVTYHKPGNVYAGLVHRLDKQVSGIMVFAKTSKAASRLFSQFKGHEVVKLYCAAVES
                     KITPCDNQWHLLEDSVVRIHSTTHVGVTKNAVPAALQYKVIYHDEPFSLVLVNLYTGK
                     KHQIRAQLSHHAMPIANDTKYGAHPTTGDNQIALHACFLQFIHPTTKKVVSFYSPPYG
                     IFTVLFKELDIDSIIKQALQQENFK"
BASE COUNT          767 a          484 c          544 g          853 t
ORIGIN      
        1 cggtaaacaa tctgcagttt gtaatcatca ttggtgtact ggcaggtatt ggcatttctg
       61 catcgcagat tattcctttt tctatgatac ctgatgtcat tgaatatgat gaatattcca
      121 gtggcatccg tcgcgaagga gctttttatg ggattacaac atttttgtat aaggttgcat
      181 caacagcggc aattgcggga gcttcagcta tgctggcata ttttggctat gtagaaaaca
      241 gcaccatggc atagccacag tcggcaatta gtgccatacg atacatgatg gcttttgcac
      301 cgggcgtatg ctttttaatt tcggtgtgtt ttgtccggat gttaccaatt acccgtgagc
      361 gttttgatga aataaagcat ttacttgatg aacgaaagaa gcaacaatga tgcgttgata
      421 ttttatgttt ggtggagatt atgaagcgtt tactatcatc aggaattatt gtcatggcac
      481 ttatcagtat agttatggtg tcctgctcca ggaaagagaa ggggcgtttt tatagtgatc
      541 aatatcattt ttcaatagta ttccccagaa catgggagat acagcaaaaa gagatgggta
      601 ccgttgttat agggcttagt cctgaagaag gattaaaaga tgatttccgc gaaaacataa
      661 ccataacaac agaggatgcc ggtgatgatg taacccttga aaaatatttt gacatatcac
      721 aaaagaatat gaaaaactat tctacagact ataagctttt atataaaaac tatacatggc
      781 ttgctaaaaa aagagccatg atactgggat ttacctatac catgagcgga ttaaagctca
      841 aagcattgca atattatatc attcataata aaagagcata cgtcattaca tgcaccggat
      901 tacctcatac ttttggacgc tatgaagaac gttttttaac aattgtgcgt tcattcaggc
      961 ttgaaaaata aaatcatctg ctggcgtagc aatacataca tccaaacctg cagctattat
     1021 atgctcctat gtcaacactt tttgtgcaca ggcagttttt acgctgatga gcatctctgg
     1081 gtacattaag ttgaatatta aaaatattcg atagcaactg ctgatctatg cacgctccat
     1141 tacttattcc atacagtgaa agctcactgc aacagctttg tatgtgcatg cctcgctcat
     1201 gtgcaatgga tgatagcgaa gaaaaaaact gttccatctg ttctgcatta taatctgcaa
     1261 aaggatttaa tactatattt ctatcgataa gatgtttggt aatgcgttta attacgctac
     1321 tgtaaggctc cattatgctg acaattactc tttgcgtatg gtggtgcagt tccgatgcta
     1381 tagccctgaa gttgttgata tgaaaatcaa aatccagttc tcgggaaaat actattgggt
     1441 catatcgcca tataaccttt tgcgctccaa ttttgttgct taacgcttta aagattttta
     1501 tgctctgcga tagttccggt gaatgagctt caagccattg aggatagccg gtgatggtga
     1561 ataaaaatat aaagtgatac ccaccgcaat ccagttcatc aaggtattgc aatagcgggg
     1621 caggatagcg agtccagaat actatcgcct caacatcttc gggcagtagc gatatctccc
     1681 ttatttgatt gggattgtat ggatttttta ccgtacaact gcccctgtgt atgcaatcca
     1741 taaaccactg cgaataaaag gcagggatgt ctgttcgccg gcttgcactt attatcatga
     1801 gctatcgcca aaactattat ttaaaatttt cttgctgtaa cgcttgtttg ataatgctgt
     1861 caatgtcaag ctctttgaat agcacggtga agattccgta tggtggacta taaaacgata
     1921 ccaccttttt tgttgtagga tggataaatt gtaaaaagca tgcatggagt gcaatctgat
     1981 tgtccccggt ggtggggtgt gcaccatatt ttgtgtcatt ggcaatgggc atagcgtgat
     2041 gcgaaagctg tgcgcgtatc tgatgttttt tgccggtata aaggttaacc agaacaagcg
     2101 aaaacggttc atcatgatat ataaccttat actgcagtgc tgctggtaca gcgtttttag
     2161 taacaccaac atgtgttgta ctgtgaatgc ggacaacact gtcttcaagg agatgccact
     2221 ggttatcgca cggagtaatt tttgattcaa cagcagcaca gtatagtttt acaacctcat
     2281 gacctttaaa ctgcgaaaat aaccgtgacg ccgcttttga tgtttttgca aataccataa
     2341 tccctgaaac ctgtttatcc aatcggtgaa caagtcctgc ataaacattg ccgggtttgt
     2401 ggtaagtgac tttaatatat tccttaatta tgtccagcaa cgactgctcg tttgttttat
     2461 ctttttgact taagatccct gctggttttt caacaacgat aatatgattg tcttcgtaaa
     2521 caatgcggat atttttatat attttcatga tgacaatgat gcagcgtgtt ttatgtatgt
     2581 tttgttaaat aattgctaca atcttcagca ttttttgtga tgtacagttc aatctctttt
     2641 atggtttt
//
LOCUS       DAOSOX010000278         2634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_38932_length_2634_cov_4.659170, whole genome shotgun sequence.
ACCESSION   DAOSOX010000278 DAOSOX010000000
VERSION     DAOSOX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38932_length_2634_cov_4.659170"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="PLX22_12365"
     CDS             <1..1590
                     /locus_tag="PLX22_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOT20732.1"
                     /translation="RLKSILWKVSNSRIKGVFTGFFITAIIQSSSATTVMLVSFVSAG
                     LLTLQQSIGIIFGANIGTTVTGWLVALIGFKIKITAIALPCIGLGFFIRFISNEKIKN
                     YGEVLLGFGVLFFGLDIMSSAVKDLRNSEAILNIMAHYSADDIPSTLIVIAIGTIVTM
                     IVQSSSATVAMTMTLAVNGLIDFPTACAFILGENIGTTITANIAALSASSEAKQAARA
                     HFLFNAIGVIWLMLIFHRFFIPFVDWLVPGNPYSDILAVRSKVIADHMAAFHTCFNVI
                     NTIVFLPFANILTNLAIKLAPQSNDVPDEFHLKYITSILVSTPTININQARLEIKRMS
                     NIVLEMFDMVMDVFHHPEEKLGTQVEQILKLENLTDHLEKEISSFLVSVLQNNISYEQ
                     SEEISSLLHTVNELERIGDNCESLLKLLRRKYEQNIQFSDEAVKGIDEIASKVREFMV
                     LINQNITVRNKDIMADSKFLEDRIDELRNELRKGHVNRLNEGVCNVDAGLMFIDMLSK
                     FEKIGDHAFNVAESISGVRIF"
     gene            complement(1570..2088)
                     /locus_tag="PLX22_12370"
     CDS             complement(1570..2088)
                     /locus_tag="PLX22_12370"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOT20733.1"
                     /translation="MKRLFIGMLFIGGLALMAFTYHNDILLRQEPWIELSNTQEKYKG
                     VTWKITAYCPGSCCNKRITGTKVVDYTNSAAIGGLKLTTLLDNTINVVAVDRSIIPLG
                     SIVKYNNNYYVALDTGSAIKGFTLDILMPHHKQTEKFGIQYSDNIEVYIPHNPAQIIK
                     KIKKIALEYSNP"
     gene            2319..>2634
                     /locus_tag="PLX22_12375"
     CDS             2319..>2634
                     /locus_tag="PLX22_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20734.1"
                     /translation="MIGNIATLVMCLAMILFFRRLDRSNLRITKLKRYTSKVLDDFKK
                     LTQDEERKFSDATIELDIMLKKAQALSTHLSSSISGIEEKLKGLDIEKTNLKKVEEDL
                     KVI"
BASE COUNT          856 a          431 c          477 g          870 t
ORIGIN      
        1 cgtttaaaaa gcattttatg gaaagttagt aatagcagaa tcaagggagt atttacagga
       61 ttttttatta ctgccatcat tcaatcttca agtgccacta ctgttatgct ggtaagcttt
      121 gttagcgccg ggcttttaac tctgcaacaa tcaattggga tcatttttgg tgcaaacata
      181 ggcactacag taaccggatg gcttgttgca ttgataggtt ttaaaataaa aattacagca
      241 atagctctcc catgcattgg gttaggcttt tttatacgtt tcatatcaaa tgaaaagatt
      301 aaaaactatg gtgaggttct tttaggattt ggtgttttgt tttttgggct tgatattatg
      361 agcagcgcag ttaaggactt gcgaaactca gaggcaattc ttaacattat ggcccattat
      421 agtgcggatg atataccatc tactctaatt gtaattgcaa ttggaacaat tgtaaccatg
      481 atagtccaat catccagtgc aacagttgcc atgacaatga cgttagcagt taatggactc
      541 attgatttcc caacagcatg tgcattcatt ttaggtgaga atataggtac aactataacc
      601 gccaatattg ctgctttgag tgcatcatcc gaagcaaaac aggcagcgcg tgcacatttc
      661 ctttttaatg ccataggggt gatatggtta atgcttatat tccataggtt ttttataccg
      721 tttgttgatt ggctggtacc cggcaatccc tattctgaca ttttagcagt acgatcaaaa
      781 gttatagcag atcatatggc cgcattccat acatgtttta atgtaattaa tactattgtt
      841 tttttgccat ttgcaaatat tcttacaaat cttgccataa aattagcccc acaatcaaac
      901 gatgtgccgg atgaattcca cctaaaatat attacctcca ttctggtatc tactcctaca
      961 ataaatatca atcaggcacg tttagagatt aaacgcatga gcaatattgt acttgaaatg
     1021 tttgatatgg ttatggatgt cttccatcat ccagaagaaa aattgggcac ccaggttgag
     1081 caaattttaa aattagaaaa tcttaccgat catcttgaaa aagagatttc aagttttcta
     1141 gtaagcgttt tacaaaataa tatttcatat gaacaaagcg aagagatatc atctctgttg
     1201 cacactgtaa atgaattaga acgcattggc gataattgtg aatcactact aaaactacta
     1261 aggcgaaaat atgagcaaaa tattcaattt tctgatgaag cagtaaaggg tatagatgag
     1321 attgcatcaa aagtacgtga gttcatggtt cttatcaatc aaaatataac tgttcgtaac
     1381 aaagatatta tggctgattc taaattcctg gaagatagaa ttgatgaatt gcgaaacgag
     1441 ttacggaagg gacatgtcaa cagactaaat gaaggtgttt gcaatgttga tgcggggcta
     1501 atgtttattg acatgctatc aaaatttgaa aaaattggcg atcatgcttt taacgtcgct
     1561 gaaagcattt caggggttag aatattctaa cgctatcttc tttatttttt taataatctg
     1621 tgctggatta tgcggaatgt atacttcaat gttatctgaa tactgtatgc caaatttttc
     1681 agtttgctta tgatgcggca ttaaaatatc cagtgtaaat ccctttattg cactgccggt
     1741 atcaagcgcc acataatagt tattgttata ctttactatt gaaccaagcg gtatgataga
     1801 acggtcaacg gcaacaacat tgatagtatt atcaagcaat gtggttagct ttaatccgcc
     1861 aattgcggca ctattggtgt aatcaacaac ttttgtaccg gtaatacgtt tattacagca
     1921 gcttcccgga caataggcgg ttattttcca tgtaacaccc ttatattttt cctgggtgtt
     1981 cgatagttct atccatggct cctgccttag caatatatca ttgtgatagg taaatgccat
     2041 caaagctaac ccaccgatga atagcattcc tataaataat cgtttcataa tttattttat
     2101 actataatta gtctatacaa ttaataataa ttatactgca ttataatgtt atttcaagac
     2161 aacgtttagc atatcctctg caagtcaatc attttttaat gtgacataaa ataatgttga
     2221 caaattttat taattgtttt atgtagtaga gtcaatatat tggctttatc tttattgcat
     2281 aaaatagcaa ttcctttaaa aaaaagaggt gcaatgcgat gatagggaat attgcaacgc
     2341 tggttatgtg tttggcaatg atactttttt tccggcgtct tgaccgttct aatttgcgaa
     2401 ttacaaaact aaaacgatat acatccaaag ttctggacga ttttaaaaaa ttaacgcagg
     2461 atgaagaacg caaattcagt gatgcaacca tagagcttga tattatgtta aaaaaagcac
     2521 aggcgttaag tactcatctc agttcctcaa tttcaggtat agaagaaaaa ttaaaagggc
     2581 ttgatattga aaaaacaaat ttaaagaagg ttgaagaaga tttaaaagtt attt
//
LOCUS       DAOSOX010000279         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_39415_length_2608_cov_5.998433, whole genome shotgun sequence.
ACCESSION   DAOSOX010000279 DAOSOX010000000
VERSION     DAOSOX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39415_length_2608_cov_5.998433"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1589
                     /locus_tag="PLX22_12380"
     CDS             33..1589
                     /locus_tag="PLX22_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT20735.1"
                     /translation="MQKKVMLGNEAIARGAYEAGCHVAAAYPGTPSTEILETIAAEYR
                     NIKAQWSPNEKVALEVVAGASVAGARAMAAMKHVGLNVAADPLFTFSYTGANGGFVII
                     NADDPGAWSSQNEQDNRHYARAAKVPMIEPTSPEESRQYTKLAFELSEQYDRPVIVRI
                     TTRIAHSQGVVTLEEPTKVDLKPFIRDPQKYVMIPAHARPRHIFIEGQMIKLKEYSNN
                     CDLNTIEWNSTKRGIITNGVAYQYVKDVCPDASILKIGVAWPLPDAKIHEFCRKIEEL
                     YIVEELDPFIEEHVKTLGYKPIGKEIIPLCGELTPEIIDMAINKKMPKNEIPLYSGNI
                     PGRPPALCKGCPHGFVFEVLKKLDVVVNGDIGCYTLAVLPPYSAMHSQGCMGASIGMH
                     SGFEKARGDEMARNSVAVIGDSTFVHSGITPLIDLVYNKGTGTVIILDNRVTAMTGHQ
                     DHPATGKTLTGEDTHELDLELLCKAVGVKRVRKLDPKNMDEFEKAVKEEIAAKEPSVI
                     ISLRKCILKK"
     gene            1600..2175
                     /locus_tag="PLX22_12385"
     CDS             1600..2175
                     /locus_tag="PLX22_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT20736.1"
                     /translation="MKSVIFAGVGGQGVILAGKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCHVCFGEKVYSPLIEKGTADFVIALELLEAMRKLEYLSTNGTLIVNKFKVNPAP
                     VEAGLAKYPDDIESWLCSNIGKCHVVDTAKVLKEAGSNKAVNIVMVGILSNFLEFDQK
                     AWEEAIRQSVKEKFLDMNIKAFHLGREIPLS"
     gene            2257..>2608
                     /locus_tag="PLX22_12390"
     CDS             2257..>2608
                     /locus_tag="PLX22_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20737.1"
                     /translation="MMRYYTFTVIICLCFIVACSSGKPKQLHENLPRMKAVNKVGILV
                     RIPASSPVEYTRYVKTLQAMLAGYKQIKEIVIVDSDTAAITTFQSNDDRFYQTSLDGD
                     FLYYKATGIVTSYCT"
BASE COUNT          847 a          386 c          610 g          765 t
ORIGIN      
        1 aaaaaaaatt gatttaattg gggagtaatg caatgcaaaa aaaagttatg ctgggtaatg
       61 aagctattgc gcgtggtgct tatgaagcag gttgtcatgt agctgcagcg tatcctggaa
      121 ctcccagtac agaaatttta gaaacgatag ctgctgagta tagaaatatt aaggcacaat
      181 ggtctcccaa tgaaaaggtt gcattggaag ttgttgctgg tgcatcagtt gctggtgcac
      241 gagcaatggc tgcaatgaag catgttgggc ttaatgtagc ggcagatcca ttatttactt
      301 tttcatatac tggtgcaaat ggtggatttg ttattattaa tgctgatgat cctggtgcat
      361 ggagttcaca aaatgagcag gacaatcgcc actatgcacg agctgccaag gttcctatga
      421 ttgagcctac aagccctgaa gagtcacgac aatatactaa attggcattt gaattgtcag
      481 aacaatatga caggccggtg attgtgcgta ttacaacacg catagcacat tcacagggtg
      541 ttgttacact tgaagaacct acaaaagttg atttaaaacc atttataagg gatccacaaa
      601 aatatgtcat gattccggct catgcaaggc caagacacat ttttattgaa ggacagatga
      661 taaaattgaa agaatattca aataactgtg atcttaatac catcgaatgg aattcaacca
      721 agcggggtat tattaccaat ggtgttgcat atcagtatgt caaggatgta tgccccgatg
      781 catcaatatt aaagatcggt gttgcatggc cattacccga tgcaaaaata catgaatttt
      841 gtcggaaaat agaagagctc tatattgttg aggaacttga tcctttcata gaagagcatg
      901 taaaaacatt aggatataaa ccaataggga aagaaataat cccactatgt ggagaattaa
      961 ctcctgaaat aatcgatatg gctatcaaca aaaagatgcc taaaaatgaa ataccgctgt
     1021 attccgggaa tattccagga agacctccag cattatgtaa aggatgtcct catggttttg
     1081 tatttgaggt gctgaaaaaa ttagatgttg ttgtcaatgg cgatattggt tgttatacat
     1141 tggcagtatt gcctccatat tctgccatgc actcacaggg ttgtatgggt gccagtattg
     1201 gtatgcattc agggtttgaa aaagcccgtg gtgatgagat ggcacgaaat agtgttgctg
     1261 tcattggtga ttccaccttt gtacattcgg gcattacacc attaatagat ttagtatata
     1321 ataaaggaac tggtacggtt attattctgg ataatagggt aaccgcaatg accggacatc
     1381 aagatcatcc tgcaacaggg aaaacgctta cgggggagga tacacacgaa ctggatctgg
     1441 aactgttatg caaagcagtt ggggtaaagc gggtgagaaa acttgatcca aaaaatatgg
     1501 acgagtttga aaaagctgta aaagaagaaa ttgcagctaa agaaccttcg gttattattt
     1561 cattgcggaa atgtattttg aaaaaatagg ggaatattta tgaaaagtgt catttttgcg
     1621 ggagttggtg gtcagggggt tatccttgcg ggtaagatat taatgcaggt tgccatgaat
     1681 gcaggatatg atgttaagga atctgaagta catggcatgg cccagcgagg cggtagtgtt
     1741 gattgtcatg tatgttttgg ggaaaaggtg tattcaccat tgattgaaaa aggtacagcc
     1801 gattttgtta tagcgctgga attactggaa gcaatgagga aattagaata tttatcaaca
     1861 aatgggacgc ttattgtaaa taaatttaag gttaatcctg cgccagttga ggcaggttta
     1921 gcaaagtatc ctgatgatat tgagagctgg ttatgtagta atattgggaa atgccatgtt
     1981 gtggatacag ctaaggtatt aaaggaagca ggatcaaaca aagcggtgaa tatagtgatg
     2041 gtagggattt tatctaattt tttggagttt gatcaaaagg catgggaaga agctatacgg
     2101 cagagcgtca aagaaaagtt tttggacatg aatattaagg catttcattt aggaagagag
     2161 atcccattat catgaactat cgccaataat attttttaaa atatgagttt aaatttatag
     2221 attttgaaac agtaatcgtt atatacaaaa ggagttatga tgaggtacta tactttcact
     2281 gttattattt gtttgtgttt cattgttgct tgcagttcgg gaaagcctaa acagctccat
     2341 gaaaatttgc ccaggatgaa agctgtcaat aaagttggga tattggtacg aatacctgca
     2401 agcagtcctg ttgagtatac acggtatgtt aaaacattac aggcgatgct tgctggatat
     2461 aagcaaataa aagagatagt tattgttgat agtgatactg ctgcaattac cacgtttcaa
     2521 tccaatgatg acagatttta tcaaacttca ttggatggtg attttttata ctataaagca
     2581 acaggtattg ttacttcgta ttgcacca
//
LOCUS       DAOSOX010000280         2599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_39615_length_2599_cov_7.727987, whole genome shotgun sequence.
ACCESSION   DAOSOX010000280 DAOSOX010000000
VERSION     DAOSOX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39615_length_2599_cov_7.727987"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="gap"
                     /locus_tag="PLX22_12395"
     CDS             complement(<1..884)
                     /gene="gap"
                     /locus_tag="PLX22_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT20738.1"
                     /translation="MGIKVAINGFGRIGRMVLRAISNDPKYADIDVCCINDLTDAATL
                     AYLFKYDSVFGVFNGTVSHKENAITINGKEIKILAEKNPATLPWKAESIDVVVESTGK
                     FRDRDKVMPHLDAGAKKVIITAPAKGEDITVVLGVNEDKYDTAKHNIISNASCTTNCL
                     APVAKVVNDVFGIEKGLMTTVHSYTNDQRLLDLPHSDFRRARAAALSMIPTTTGAAKA
                     VALVLPELKGKLDGLSIRVPTPDVSVVDFVCTVKTQTTAEDVNKALREAANGKLKGIL
                     QVCDEELVSIDFKGNNHSS"
     gene            complement(1000..>1437)
                     /locus_tag="PLX22_12400"
     CDS             complement(1000..>1437)
                     /locus_tag="PLX22_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20739.1"
                     /translation="GALFLATAGKLPVNPVVFDLLDKDFDGLCKLAVDLSYGAIDDTM
                     RKTAIECMKQAGSNVLKNDFTLCNSYNYDETAAQFPVASCFIANRKDTMVPLDIIYAT
                     FKKMPDAMLQVLPYKGHMSHIEQVHQVAACINHFMEFVKSKNF"
     assembly_gap    1438..1537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1538..2186
                     /locus_tag="PLX22_12405"
     CDS             <1538..2186
                     /locus_tag="PLX22_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20740.1"
                     /translation="LLQNILFSWQLFLFIITICAIVTAYEQCRNDNPNYQHNSLPGIL
                     LTIKVSEFTIMHTITSACQPGFNASCALCCGSHNYNAEYHEIDNLFKERFYCFNTFIS
                     TDNIHSMLTACYNAMKQITLPPRDDDATHCAFVAYTDTSFTTIGCLLYPYCYRFDYRS
                     LHMGNICKTFNCKVNTPAYHDTVITAATIFHDWYYYSIAIHNTALLNNIHRMCMA"
     gene            complement(2344..>2599)
                     /locus_tag="PLX22_12410"
     CDS             complement(2344..>2599)
                     /locus_tag="PLX22_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20741.1"
                     /translation="DLSKEIEKVRADLSKEIEEVRADLSIDIEKSRAETSISLGKLRS
                     DLIEKIEEKHSSSIRWLVALFITQSVGVAGLIVTLFQILR"
BASE COUNT          782 a          539 c          400 g          778 t
ORIGIN      
        1 gatgaatgat tattgccctt gaaatcaatc gataccagct cttcatcgca tacctgtaaa
       61 attcctttta gcttaccatt tgcagcttcc cgcaaagcct tattcacatc ctcagcggtt
      121 gtctgagttt taaccgtaca tacaaaatca accactgata catcaggggt ggggacacga
      181 atggacagcc catcaagctt acctttgagc tcgggcaata ctaaagcaac tgcctttgct
      241 gcgccagttg tggttggaat cattgaaaga gctgctgccc gtgctcttct gaaatccgaa
      301 tgaggaaggt ccaatagacg ctggtcattt gtatatgaat gcaccgtggt cataagccct
      361 ttttcaatac caaatacatc attaacaact ttagcaaccg gcgccaaaca gtttgtagta
      421 catgatgcat ttgagataat attatgtttt gctgtatcat atttgtcttc gttaacgcca
      481 aggacaacgg ttatatcttc acctttagcc ggtgcagtaa taattacctt tttagcaccc
      541 gcatctaaat gcggcattac cttatcacgg tccctgaatt ttccggttga ttcaacgacc
      601 acatctatac tttctgcttt ccacggaagt gtagctggat ttttttctgc caatatcttt
      661 atttctttac cattaatagt gatggcattt tctttatgac ttactgtccc gttaaaaaca
      721 ccaaaaaccg aatcatattt aaaaagatat gccagtgttg ctgcatcggt taaatcatta
      781 atacagcaaa catcaatatc agcatattta gggtcgtttg atatagccct aagcaccatt
      841 ctgccaatcc ttccaaaccc gtttatggca accttgatac ccataataac ctcctcgtgt
      901 tttttaaatt aacaaatcta tgaacatact aaaaactatg attacacaaa acaatatata
      961 ctgattttca aaaattatcg cataaaaatt ttttaaaaat taaaaatttt ttgatttcac
     1021 aaactccata aaatgattga tgcaagctgc aacctgatga acctgctcaa tatgtgacat
     1081 gtgtccttta tagggcaata cctgtaacat tgcatcaggc atttttttaa atgttgcata
     1141 gataatatca agcggcacca ttgtatcctt acggttagct atgaagcacg atgcaacagg
     1201 aaactgtgct gccgtttcat catagttata gctattgcat agagtaaaat catttttcag
     1261 aacattactg ccagcctgct tcatacattc aatagctgtt ttacgcatcg tatcatcaat
     1321 ggcaccatag cttaaatcaa cagcaagctt acataaccca tcaaaatctt tatccagaag
     1381 atcaaataca acaggattta ccggtaactt cccggctgtt gctaaaaata gtgctccnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt gttgcaaaat atcctgtttt
     1561 catggcaatt gttcctgttc attattacaa tatgtgcgat agttactgcg tatgagcaat
     1621 gccgtaatga taatcccaac tatcagcata attctttgcc cggaatacta ttaaccatta
     1681 aagtttcaga atttaccatt atgcatacaa ttaccagtgc atgtcaacca ggatttaacg
     1741 catcgtgcgc tttatgctgc gggagccata actacaatgc agaataccat gaaattgaca
     1801 atctttttaa agaacgcttt tattgtttta atacattcat atctacagac aatatccatt
     1861 ccatgctgac agcatgctat aacgctatga aacaaataac attgcctcca cgtgatgatg
     1921 acgctacaca ttgtgcattt gttgcatata ccgatacaag tttcaccaca ataggttgcc
     1981 tgttatatcc atactgttac aggtttgatt accggagcct tcatatgggg aatatatgta
     2041 aaacatttaa ctgcaaagta aatacaccgg cttaccatga taccgttata acagcagcaa
     2101 ccatctttca tgactggtac tactatagta ttgccataca caatacagca ttgttaaaca
     2161 acatacacag gatgtgcatg gcataacgtt aacactaact aaaagggaat ttcattatac
     2221 ctgctgcgag ctgagcgtta tatggatatt gtaagatgta aagtgtaaaa tgaaagatga
     2281 aagatggtaa atggtatgtg taagcctgcc tctgcaatta ttatcatctt tgcgatacag
     2341 ctatcaccgc aaaatctgga acagcgttac aattaagcca gcaactccaa ctgattgagt
     2401 tataaacaaa gctaccaacc atcgtattga agaagaatgt ttttcttcaa tcttttctat
     2461 taaatcagat ctaagctttc ccaatgaaat agaggtttca gcacgggatt tttcaatatc
     2521 aattgataaa tccgctcgta cctcctctat ctcctttgat aaatccgctc gtaccttctc
     2581 tatctccttt gataaatcc
//
LOCUS       DAOSOX010000281         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_39872_length_2586_cov_7.961675, whole genome shotgun sequence.
ACCESSION   DAOSOX010000281 DAOSOX010000000
VERSION     DAOSOX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39872_length_2586_cov_7.961675"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..>2586
                     /locus_tag="PLX22_12415"
     CDS             354..>2586
                     /locus_tag="PLX22_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT20742.1"
                     /translation="MAFSYANNDYNNTVKQNVRIVTSLYEPFVMFKDGKLVGFDIDLI
                     NEICKQNHLTYSITITSFQDIFTQIEKGTADMAIGCIYVTEEREEIFQFSKSYLEGGL
                     VLVVPANSDIKTIYELNNKKLGVKKDATGDLFASKIVQKGYSIEIIRYQDTVDSFQAL
                     VNGSVDAVLNDYLNSVYLLNKYFSGDIKIVRGRWSDIIYGRRGIAYPISSNNQHLYEI
                     INKSIHDLKSSNFIDMLYWKWFAIMPPPNIIRRVMIYTGLVAVSIGITCLLFLHFNKR
                     KKFKELSMIEEHFRNLINDLNVAVYIIQNNSIVLTNHEGAHIAGSSIESIIGYPLDYL
                     FSEYTVINDKELHYSSFTDLSNMVQAGPMVYRARWKRKDGTVLDVLFRIRHVQWMGNS
                     ALECIVNDTTDINRLLVEREKLKGQLLQSQKLEIVGQITGGIAHDFNNILTVILGYAN
                     LLEASVDAKNREAVTKIIEAGNHARDIVSKMLTFARKKVFLEQKMHLHHIIQNSILLL
                     KTALPKSCTVDMQLHATVDTIVGDTTQIQQMLMNLVINASEAMPEGGTINIETTNVTI
                     EHERIGKVETIHAGEYILLSIKDSGIGIAEKDIEHIFEPLFTTKEKGSGFGLSIVYGI
                     VKQHKGYIDVVSIPGKGSTFYIYLPVITENRVEASLKIIDNKKESLPALGGEIVIIDD
                     DANIGSLYKESLHLAGLHAVTFINAEEMFDYIRTHAITVKLIISDVRMPGISGEQVLE
                     YIKQ"
BASE COUNT          900 a          390 c          509 g          787 t
ORIGIN      
        1 agtaaaattt ttcctttgta aaaaaaattt aataaaaaat aacttaaaaa agccatattc
       61 taggtattca tgttggttac aagatgtaac actattaact gttactccag cccccattaa
      121 agctgcgaat tgtcgcacgt ttttaatgta aattttctcg ggattccgta aaggaatccc
      181 ttttttttaa ttgtatgata tgtatagaga agaattaaaa aatatggtag cattgaatat
      241 tgcattatag cataaatacc attgacattt tgataatggc gttgtaaaaa atactcatga
      301 aacacttgct caaatattgt tgtgtattac tgttaatgaa tcctggcaat gccatggcat
      361 tttcctatgc caataatgat tacaacaata ccgtaaaaca gaatgtacgc attgttactt
      421 cgctgtacga gccatttgtt atgtttaaag acggtaaact ggttggattt gatatagatt
      481 taatcaatga aatttgcaag cagaatcact taacctattc cataaccata acatcatttc
      541 aggatatatt tactcagatt gaaaaaggca cggctgatat ggctattggc tgtatctatg
      601 ttacagaaga gcgtgaagag atatttcaat tttccaaaag ctatcttgag ggagggttgg
      661 tgctggttgt gccagcgaat agtgatatta aaaccatata tgaattaaac aataaaaagt
      721 taggggtaaa aaaagatgca acgggtgatt tgtttgccag taaaatagta caaaaagggt
      781 attccattga aattatacga tatcaggata cagttgattc atttcaagca ctggttaacg
      841 gcagtgttga tgcagtgctc aatgattatc taaactcggt gtatttatta aataaatatt
      901 tttcaggtga tataaaaatt gttaggggta gatggagtga catcatctat ggtcgcaggg
      961 ggatagcgta tcctatatca agcaacaatc agcatcttta tgagataatc aataaaagta
     1021 tccatgattt gaaatcaagt aattttatag acatgctata ctggaaatgg tttgcaatca
     1081 tgccgcctcc aaacattatt cggagagtga tgatctatac aggattggtt gccgtatcca
     1141 ttggtataac atgtttacta tttcttcatt ttaataaaag aaaaaaattt aaagagctat
     1201 ccatgattga ggagcatttc aggaacctca tcaatgatct taatgttgct gtttatataa
     1261 ttcaaaataa tagcatagta ttaaccaatc atgaaggtgc acatattgca ggaagctcca
     1321 ttgaatccat cataggctat ccacttgatt atctcttttc ggaatataca gtaataaatg
     1381 ataaagaact tcattattcc tctttcacag atttaagcaa catggtacag gcaggtccca
     1441 tggtttatag agccagatgg aaaagaaaag atggaacagt actggatgta ctttttagaa
     1501 ttcgccatgt acaatggatg ggcaatagcg ctcttgaatg cattgtcaat gatactaccg
     1561 atataaacag attattagtg gagcgcgaaa agctgaaagg acagttgttg caatcacaaa
     1621 aattggagat agtaggtcag ataacaggcg gcattgcaca tgactttaac aatatattaa
     1681 ccgtcatact tggctatgct aatctgcttg aagccagtgt tgatgccaaa aatagagagg
     1741 cggtaacaaa gatcattgaa gctggtaatc atgccaggga tattgtttca aaaatgttaa
     1801 cctttgcacg gaaaaaagtt tttctggagc aaaagatgca tttgcaccat atcattcaaa
     1861 atagtatact attattgaag acagcattgc ctaaaagctg cacagttgac atgcaattac
     1921 atgcaaccgt tgataccata gtaggcgata caacacagat acagcaaatg ctgatgaatc
     1981 ttgtcatcaa tgcttcagaa gcaatgcccg aggggggaac aatcaatatt gaaacaacta
     2041 atgttaccat agaacatgag cgtattggga aggttgaaac catccatgca ggtgagtata
     2101 ttttactatc tattaaagat agcgggatag gtattgctga aaaggatatt gagcatatct
     2161 ttgagccttt atttactaca aaagaaaaag gaagcggatt tggtctgtcg atagtttatg
     2221 gtattgttaa acagcataaa ggatatattg atgtagtatc aatccccgga aaaggaagca
     2281 cattttatat atatttacca gtcataactg aaaatagagt agaagcatcg ttgaaaatta
     2341 ttgacaataa aaaagaatca ttaccagctt taggtggtga aatcgtgata atagatgatg
     2401 atgctaatat tggttcttta tataaagaaa gcttgcatct ggctggttta catgcggtaa
     2461 cttttatcaa tgcagaggag atgtttgatt acattcgcac tcatgctatc acggtaaaac
     2521 ttattatcag tgatgtgaga atgccaggga tttcgggtga acaggtgctg gaatatataa
     2581 agcagc
//
LOCUS       DAOSOX010000282         2568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_40254_length_2568_cov_8.062077, whole genome shotgun sequence.
ACCESSION   DAOSOX010000282 DAOSOX010000000
VERSION     DAOSOX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40254_length_2568_cov_8.062077"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..552
                     /locus_tag="PLX22_12420"
     CDS             79..552
                     /locus_tag="PLX22_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20743.1"
                     /translation="MQQFDCNETSIVYYPEKYIIVCIAYGDGKKVWAKKMHEPVAIQN
                     VLFDERYYYIACKTGDTEGIFLTLDKSNGSTVWFIPGCTSLEVLYRGYLYLIFVDDNE
                     QHFLIKVECDEGNKLWFHQIDSDLYYYHFKEDCIILHYASGKKEMLQYDGKVLLR"
     gene            673..1527
                     /locus_tag="PLX22_12425"
     CDS             673..1527
                     /locus_tag="PLX22_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20744.1"
                     /translation="MKAIGIPTEAASIYNKALALSNMGNYDLAIEQYMRAVEVFPQFI
                     EAYNNIGELYSRMGNSEKAIQVYSKALSIKKNHKVLLNLGVEYYNAADYTIALQYFNE
                     SLLLKKDFLEGNFYTGMTYFNLKNYVSAKKYFEATVAIDAHHLKANYLLAYILYEDKE
                     YLKVIECLKRIEHIADDLMFINRYYGFCYYHLGDYKKAVKFLSAALTNSPKYAQFKAY
                     LESLTYENKLKEIGDIDATIQALERKIMAEKPNLSEYTRLSMLYIFKGQYKKAEDLLV
                     GVKSKIAR"
     gene            complement(1563..2048)
                     /locus_tag="PLX22_12430"
     CDS             complement(1563..2048)
                     /locus_tag="PLX22_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT20745.1"
                     /translation="MVTEGKYVAIHYTGTFDDGQVFDSSLDGTPFEFQIGAGMVIPGL
                     DKGVVGMKLDEEKDIVVIPEDGYGDYDENLLYAFPLEEVKQQFKPEIGMTIALQMDNG
                     SQIPAVIKEIGDDEVVVDMNHPLAGKTLHFHIKVINISDSPEYSNGCDACGGSHDGCS
                     C"
     gene            complement(2305..>2568)
                     /locus_tag="PLX22_12435"
     CDS             complement(2305..>2568)
                     /locus_tag="PLX22_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20746.1"
                     /translation="IYAQPVNSSQITQELSPILTMLIIQLKPFTGKLPIKKLLIHFTV
                     DENKKPVGGATVFIWNSNEKCFYSQSEAWGPEGTVMSVHKLKK"
BASE COUNT          830 a          429 c          496 g          813 t
ORIGIN      
        1 gcaattgacg aattagcacg tcttataatt gagactatga agcacaaagg tatattagtg
       61 taagtttgaa gttaaattat gcaacagttt gattgcaatg aaacatctat tgtatactat
      121 cccgaaaaat atattattgt ttgtattgcg tatggtgatg ggaagaaggt atgggcaaaa
      181 aaaatgcacg aaccagttgc aatacagaat gtcctttttg atgagcgata ttactatatt
      241 gcctgcaaaa ccggtgatac agaaggtata tttttaacat tagacaaaag caacggctct
      301 actgtatggt tcataccggg ttgtaccagc ctggaggttt tgtacagggg gtatctgtat
      361 cttatctttg ttgatgataa tgagcagcat tttttaatta aggttgaatg tgatgagggc
      421 aataagctgt ggtttcacca gatagatagt gacctgtatt attatcactt taaagaagat
      481 tgtataatac tccattatgc atcaggcaaa aaagagatgc tacagtatga cggcaaagta
      541 ttgttgcgat agtattattt accagtaact atcgccaaaa attgctgtaa ggatttctga
      601 atttctaaat gaaaatgaaa aatgtaccga tagttatcaa tagaagattt tagcatagta
      661 tgaggtttta ttatgaaggc aataggaata ccaactgaag ctgccagtat atataataag
      721 gctctggcgc tgtccaatat gggcaattat gatcttgcta tagaacaata tatgcgagct
      781 gtagaagtat tccctcaatt tattgaggca tataataaca ttggtgaact gtattcgcga
      841 atgggtaata gtgaaaaagc aattcaggta tatagcaagg cattgtccat aaaaaagaat
      901 cataaggttt tgcttaatct gggtgttgaa tattataatg cagctgatta tacgatagcc
      961 ttgcaatatt tcaacgaatc gctattgctg aaaaaagatt ttcttgaggg taatttttat
     1021 actggcatga cgtattttaa tcttaaaaat tatgtgtcgg caaaaaaata ttttgaggct
     1081 actgtcgcca ttgatgccca tcatttaaag gcaaactatc ttttagcata tatattgtat
     1141 gaggataagg aatatctgaa agtcattgag tgcttaaaga ggatagagca tattgcagat
     1201 gatctcatgt ttattaaccg gtactatggt ttttgctact atcatttagg tgattataaa
     1261 aaggctgtga agtttttaag tgctgcactt accaacagtc ccaaatatgc ccagtttaaa
     1321 gcatatcttg aaagtcttac gtatgaaaac aagctgaaag agattggtga tattgatgct
     1381 acaatacaag cactggaacg gaagataatg gcagaaaaac caaatctgtc agaatataca
     1441 cgactcagta tgctctatat atttaaaggt caatataaaa aagcagaaga cctgcttgta
     1501 ggtgtgaagt caaaaatagc aagataataa taacccacgg gttaacccgt gggttgtacg
     1561 tgttaacagc tacagccatc atgggagccg ccacaagcat cacatccatt gctatattca
     1621 ggtgaatcgc ttatattaat gactttaatg tggaaatgca atgttttccc tgccaatgga
     1681 tgattcatat caacaactac ctcgtcatcg ccgatttctt taatgacggc gggtatttgt
     1741 gaaccgttgt ccatctgcaa agcaatggtc atgccaattt caggtttaaa ctgctgtttc
     1801 acctcttcaa gcgggaaagc atatagtaga ttttcgtcgt agtcaccata accatcttcg
     1861 ggtattacaa cgatatcttt ttcttcatca agtttcatgc caaccactcc tttgtccaga
     1921 ccggggatta ccatgccggc acctatctgg aattcaaacg gtgtaccatc aaggcttgaa
     1981 tcaaacacct gaccatcatc aaacgtgccg gtgtagtgaa tggctacata tttaccttct
     2041 gttaccataa aaactcctta ataattataa ttggcaattg ctcattgtat caagtaacgg
     2101 tgctgtaatc aaataggtaa tatgaagaac tatagaatga ttaaagcatt atattgttac
     2161 agcattttac aaaaagcaat acatccttac cacaatacaa tacactatgt tgatgtcaat
     2221 atcaatattt tttacaacaa ttacatgcta ttgatgagaa aaaaaataag ctgtcatcag
     2281 ttgttatgac agctctggaa ctgttcattt ttttagtttg tgcacgctca taacagtgcc
     2341 ctcaggcccc catgcttctg actggctata aaagcatttt tcgttgctgt tccatataaa
     2401 aacagtagct cccccaactg gctttttatt ttcatcgaca gtaaagtgta tcagtagttt
     2461 ttttatcggc aattttccag tgaatggttt taactggatt atcagcattg tcaggattgg
     2521 tgatagttcc tgtgttattt gacttgaatt cacaggttgt gcatatat
//
LOCUS       DAOSOX010000283         2559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_40436_length_2559_cov_8.023562, whole genome shotgun sequence.
ACCESSION   DAOSOX010000283 DAOSOX010000000
VERSION     DAOSOX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40436_length_2559_cov_8.023562"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..605
                     /locus_tag="PLX22_12440"
     CDS             315..605
                     /locus_tag="PLX22_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20747.1"
                     /translation="MKAGLIISGSGALIYLTSYDSFMNDKLKEKFAAKGINKFIAYEI
                     PVEEAKKRYGGHFDIVVSDLHESDDLRILDYDGSRAFKLFSFQELGKPIYQE"
     gene            complement(643..>2559)
                     /locus_tag="PLX22_12445"
     CDS             complement(643..>2559)
                     /locus_tag="PLX22_12445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT20748.1"
                     /translation="LWYIILNWVYDNNIWGFSQSLAEKIGLLTAGQTFDISLLSHEAF
                     KHGKLVEITTTMITFGLGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRN
                     PATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGAALPLIGIENINAVIAPMIVA
                     GLGILFSVIGIFFVRTKEDASMKTLLAALNRGVWGSSFLLIIALAVLAHVNIITWGVF
                     GSSIAGLVAGLVIGKSTEYYTADDYAPTQGIAKQHVMGPATGILDGISVGMMSTAIPV
                     VTIVIGILAAYGLAGGFGSDLHAVSRGLYGIAFAAVGMLSTLGITLATDAYGPIADNA
                     GGNAEMSGLGKEVRTKTDALDMLGNTTAAIGKGFAIGSAALTAMALIAAYIEEVKMWI
                     SKYAAQSIDGFFQVGTIKFYNFMPENVTADLNNVLIKNATIGDFVRAYDITLLNPMVI
                     AGLFLGAMMAFLITALTIKAVGKAAGAMVEEVRRQFKEFPGILKGTQKPDYARCVMIS
                     TAGAQKKMILPSILALTVPIAAGLLLGVAGTTGVLAGALAAGFSLACMLNNAGGAWDN
                     AKKFIEKGNYGGKGSDPHKAGVIGDTVGDPFKDTSGPSLNILLKLMSMVSVVFAGVVV
                     HFYPQIAKLLGLMK"
BASE COUNT          862 a          523 c          429 g          745 t
ORIGIN      
        1 taaaaatcaa gcattaaaag taaaagcaca ccaataaatg gtgcgcttta ttgttctatc
       61 tttagctgct cggtatgtat cagttttgtt caatgctaaa gatacgaaag tagtacaatt
      121 acaatatatc aataatagat acattatcta aaaattatta tgatactttg ttgcatgttt
      181 gtaatagtaa tgtttaaaag tatttccaat ttttaaaaaa attttttaat attatagtat
      241 agtattgatt tatgtatact ttataaatat atttaataaa aaataataat attctttata
      301 aaggaggttg tgctatgaaa gcgggactta ttatatcagg gagtggtgct ttgatatatt
      361 taacatcata tgatagtttt atgaatgaca aacttaaaga aaaatttgct gctaaaggta
      421 tcaacaaatt tattgcctat gaaattccag ttgaggaagc aaaaaaaaga tatggagggc
      481 attttgatat tgtagtaagt gatttacatg aatcagatga cttaagaatc cttgattatg
      541 acggttcaag agcatttaaa ttattttcat ttcaagaatt aggaaagcca atatatcagg
      601 aataaaaaag aaaggggctt aaaagcccct ttcttttttt aattatttca ttaatcctaa
      661 tagtttagca atttgcgggt aaaagtgaac aacaactcca gcaaaaacta cagataccat
      721 actcataagc tttaaaagaa tattaagtga tggtccggaa gtgtccttaa atggatcgcc
      781 taccgtgtca cctataacac cagccttatg cggatcagag ccttttccac catagttgcc
      841 tttttcaata aatttctttg cattatccca tgcaccaccg gcgttattga gcatacaagc
      901 aagtgaaaaa ccggctgcca atgcacctgc taatactcct gttgttcctg caacacccag
      961 cagtaaacca gcagctatag gtactgttaa agctaatatt gaaggaagta tcatcttttt
     1021 ttgtgcacct gctgttgaaa tcataacaca acgggcataa tctggtttct gagtaccttt
     1081 caaaatacca gggaattcct tgaattggcg tcgcacttct tcaaccattg cgcctgcagc
     1141 cttgccaact gctttaatag taagagcagt aattaagaaa gccatcattg ctcccaggaa
     1201 caatccagct ataaccattg gatttaacag tgtaatatcg tatgccctaa caaaatcacc
     1261 aattgttgca tttttaatta aaacgttatt taaatcagct gttacatttt ctggcataaa
     1321 attataaaat tttatggtgc caacctgaaa aaagccatct atactttgtg cagcgtactt
     1381 actaatccac attttaactt cttcaatata tgcagcaata agcgccatag cagtaagagc
     1441 cgcagaacca atagcaaaac ctttccctat cgcagcggtg gtattaccca gcatatcaag
     1501 cgcatcagtc tttgttctta cttctttgcc caacccgctc atttcagcat tgccgccggc
     1561 attgtcagct attggaccat aagcatcggt tgccagcgtg atacctaatg tggagagcat
     1621 accaactgca gcaaatgcaa taccataaag ccctcttgat actgcgtgca gatctgatcc
     1681 aaatccccct gccaatccat aagcagctaa aatacctatt acaatagtaa caaccggtat
     1741 tgcagttgac atcataccaa ctgaaatacc atcaagaata cctgttgccg gacccattac
     1801 atgctgtttt gcaataccct gagttggcgc gtaatcatct gcggtataat attcagtaga
     1861 ttttccaata actaagccag ctaccaaacc ggctattgaa gaaccaaaaa caccccatgt
     1921 tattatgttg acgtgtgcaa gaacagccag agcaataatg agtaaaaatg agcttcccca
     1981 tacaccacgg tttaatgcag caaggagagt tttcattgat gcatcttctt tagtccttac
     2041 aaaaaagata cctataaccg agaacagaat acccaatccg gcaacaatca taggagcgat
     2101 aacagcattg atattttcaa tgccaataag cggcagtgca gctcccaatg cagcggtaga
     2161 aagtatagaa ccgcaatatg attcataaag gtcggcaccc atacctgcaa cgtcacctac
     2221 attatcacca acgttatcag ctattgtagc aggattgcgt ggatcatcct caggtatgcc
     2281 ggcttcaact ttaccaacaa ggtctgcacc tacatcggca gcttttgtat aaataccgcc
     2341 accaacacga gcaaaaagag cctgtgttga agcacctaaa ccaaaggtaa tcatggtggt
     2401 tgtaatttca acaagcttac catgcttgaa agcttcatga cttaacaatg aaatatcaaa
     2461 agtttgtcca gcagtcaaaa gaccaatttt ttcagctaat gactggctaa atccccagat
     2521 attgttgtcg taaacccagt ttaaaataat ataccataa
//
LOCUS       DAOSOX010000284         2544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_40757_length_2544_cov_7.729610, whole genome shotgun sequence.
ACCESSION   DAOSOX010000284 DAOSOX010000000
VERSION     DAOSOX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40757_length_2544_cov_7.729610"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLX22_12450"
     CDS             <1..355
                     /locus_tag="PLX22_12450"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT20749.1"
                     /translation="DFCGVRSGKKYDKFSIAHLTRLPAEKVKAPLIEECPISIECKVI
                     KILNLGSHNQFIGKVLLVHVDDALLDKRGALHLERAGLLCYNHGFYCAVDKQIGKFGF
                     SVKKRKKEKTKSTLR"
     gene            complement(376..846)
                     /gene="bfr"
                     /locus_tag="PLX22_12455"
     CDS             complement(376..846)
                     /gene="bfr"
                     /locus_tag="PLX22_12455"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOT20750.1"
                     /translation="MKGDPKLIETLNALLADELTAINQYMVHSEMCDNWGYEKLHKHF
                     EKRAIDEMKHAEKLIGRILFLEGIPIVTNLKKILIGADVPKQLANDHAAEEDAIKAYN
                     NAIALAGDVKDFATREVLEKILNDEDMHIDAIEELQDQINQMSLQIFLTTQVKE"
     gene            complement(1518..1736)
                     /locus_tag="PLX22_12460"
     CDS             complement(1518..1736)
                     /locus_tag="PLX22_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20751.1"
                     /translation="MERAESLFSQIVTPTDDTPKKCKAPQLNFKGGYGKYFGSNYLGY
                     RTDFSMIFKILCFDSNSVFLSLILFIQQ"
     gene            complement(1723..2226)
                     /locus_tag="PLX22_12465"
     CDS             complement(1723..2226)
                     /locus_tag="PLX22_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20752.1"
                     /translation="MSEGNNITLFVRYIVLISCIFTIVCIHTNHGISNVNNNLTGNKN
                     TVQFLSDAGLLIEGRIFAKSDAGFTSLHQKMIDLTIFTFNDYQCSFTVKEGIYTTERF
                     SNYFYPYRIEYQMDYFYFSKHFNSSSIGIAIDHKCYNTFDKTDNTGNIEFRWYGIGIK
                     WESNGKS"
BASE COUNT          882 a          377 c          381 g          904 t
ORIGIN      
        1 agatttttgc ggtgtgcgtt ctggaaaaaa atatgataaa ttttcgatag ctcatcttac
       61 caggctccca gcagaaaaag taaaagcgcc actcattgaa gaatgcccta tttcaataga
      121 atgtaaggta ataaaaattt taaatttagg cagtcataat cagtttattg ggaaagtttt
      181 gctggtccat gtggatgatg cattattaga taagagaggt gctttgcacc tggaaagggc
      241 aggactactg tgttataatc atggttttta ttgtgcagtt gataaacaaa taggaaaatt
      301 tgggttttca gttaaaaaaa gaaaaaaaga aaaaacaaaa tccacgctac ggtaagcagc
      361 gtggaatata aaagattatt ctttgacctg tgttgttaaa aatatctgta atgacatctg
      421 atttatctga tcctgcagtt cttcgattgc atcaatatgc atatcttcat cgttcaatat
      481 tttttccagt acctcacgtg ttgcaaaatc ctttacatca cctgccagtg caattgcatt
      541 attatacgct ttaatagcat cttcttcggc agcatgatca tttgccagtt gttttgggac
      601 atcagctcca attaagattt tttttaaatt tgtaacaatt ggtattcctt caaggaacaa
      661 tatcctgccg atgagttttt cagcatgttt catctcatca atggctcttt tttcaaagtg
      721 tttgtggagc ttttcatatc cccagttatc gcacatttct gagtgaacca tatattgatt
      781 gattgctgtt aattcatcag caagcaatgc attcaatgtc tcaatcaatt ttggatcgcc
      841 tttcataatt attctccttt tataaaaaaa ttttaagtat tttatatata tatacaggta
      901 atactattta ttcaagcata aaataggtta actaatgatt aaaatttacg ttatgaaata
      961 tatacattat tagagttcaa ttctgattcc aatcagatga tgtttttcgg aaataccgcc
     1021 tacggtatcg atattaatta ttttctgaaa acgatagtaa actgacagca ttacataggt
     1081 tgggaaataa accccacttt caacagcgta aaaggtatcg tttttgttat taaccaatat
     1141 gttataactg gatgaaatgt atggagttat atagttgagg ttaatgatat tatattgtaa
     1201 tcctattcta atgtcacaaa tatatgggta gtttttttta ttgaacaaat aaaatattgt
     1261 agtataccac tcagattgtt tatgatagtt tcttttcaat gattgtctat tagctatcgt
     1321 gccggtacta tttcctttgc ttgctaaaat aaatccaggt aaatgatatg tttcactaat
     1381 gccattataa aaatcaccct cataattacc actataacca tagataaatc cggtaaataa
     1441 ataatctgta aaataaatat cagctcctaa ttgcagatta taatttataa atctgggata
     1501 aagggcattt tcttcattta ttgttgaatg aacaatatca gagataaaaa aactgaatta
     1561 ctgtcaaaac ataatatttt aaaaatcatt gaaaaatcgg ttctgtatcc cagatagtta
     1621 cttccaaaat atttaccata acctccttta aaattgagct gtggtgcttt acactttttt
     1681 ggagtatcat ccgtcggagt tactatctgt gaaaaaaggc tttcagctct ttccattgga
     1741 ttcccattta atgccgatgc cgtaccatcg aaattctata ttgcccgtat tatcagtttt
     1801 atcaaaagtg ttgtaacact tatgatctat tgcaatacct atagatgatg agttaaaatg
     1861 tttagaaaaa taaaaataat ccatctgata ttcaattcta taaggataaa agtaattgga
     1921 aaagcgctca gtagtataga ttccttcttt aacagtaaaa gaacactgat aatcattaaa
     1981 tgtaaagata gtcaaatcta tcattttttg atgcaagctg gtaaaaccag catcactttt
     2041 tgcaaaaatc ctcccttcaa taagaagacc agcgtctgaa agaaattgta cggtattttt
     2101 atttccagtt aaattattat ttacattgct tataccatga ttggtatgaa tacatactat
     2161 tgtaaaaata catgaaatta atacaatata tctgacaaat aatgtgatgt tattaccttc
     2221 gctcatttta aagcgtgata tttttataaa aaaataataa tgctctatta tggaaactaa
     2281 ttgcaaaaaa tgtaaagata ataaaatacc atattcacaa aatggtgatt ttttatgaag
     2341 tataattttc attaaaataa aataaattat gatataattg acttgacata ttaaatatac
     2401 caattaataa taatgacaaa atttaccatt taataattat tatgaagggg gatattatat
     2461 gaagtcttta aaaagttcgc caacaattat tgtattaatt attgttgcac tttgtctggg
     2521 tgtagcggtt gcttatgctt taac
//
LOCUS       DAOSOX010000285         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_41596_length_2505_cov_10.393878, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000285 DAOSOX010000000
VERSION     DAOSOX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41596_length_2505_cov_10.393878"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLX22_12470"
     CDS             complement(<1..257)
                     /locus_tag="PLX22_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20753.1"
                     /translation="MNSHRITGIIRHYTRKARDQIYNAGLIIFKEAGIVEHLDDISAC
                     VDELIKNAVKANYKHLLIIQALQRQFQLQYKDKSQSQIHEY"
     gene            complement(263..>2505)
                     /locus_tag="PLX22_12475"
     CDS             complement(263..>2505)
                     /locus_tag="PLX22_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20754.1"
                     /translation="TVIAGLSLLFTEPYKKTIRAIEDCIVKKYPLRDGGFRQIVMDDP
                     SFAAVLLTHVIKRFEVSLTDVKRYTLLYQNLCRINDNFSLIAGELADNLTDTLSQRAD
                     DIYKTYTQNGGDFPSRIDAKFIVQDNSNLLKKKYTFPGLPVENLVDIKQCNFLKKIVT
                     MDNQLLITMFRNDPTIPVYMFNCISENLVKLLDRIESIHNEIDVEMDYLFGKDGFIAY
                     LIDKQKFKLWVASGRVSDGFLKGFMSIANKIYQFYQEISGENLSKKFDSFKKLHNYIQ
                     ITQDQPKVAEEVQRVSRISQNISQLYKNSVYQIFEFGFIEKENQKNLLKLLSDFKKMK
                     DPLSSEIDARKVRRQIARFYHDIFAKVFVRSQTESVVPPPVKLMLRFGFFDETMIEQE
                     QLNELHEISMRRVEQTQTPIVYEDEFLTRIYNGDENPSINEMGLSYEAHLREEQKHIK
                     GSSNIDATDNVKKIIYEINHRIATATSVCSGSTATSFPILTSLNMKGSISHYYVSKKK
                     LESIVSALRDVDFSVFYRETAVMLGNTKELIKEEVVPYFILLPSFGSKTMLWQELDGT
                     NRRTRGRIVVPILFMGDMMPTMAHTFATFRWELNRTIMGGMWADPIEGGLTGAYFDYI
                     NTFKKNSKLSLEAKQKVVERFKSIRNNRDRFADDYMQWVLYEKDGIMKLNNVVRDMFY
                     RHIPFKKELRDRLENMPAYTEIAGRFRNVFNREVGNYERKFKKYQREDGTLPEALQKF
                     MEFLYK"
BASE COUNT          783 a          496 c          363 g          863 t
ORIGIN      
        1 atatattcat gtatctggga ctgactttta tctttatatt gtaattggaa ttgacgctgt
       61 aacgcctgaa taatcagaag atgtttataa tttgccttaa cagcattttt tattaattca
      121 tcaacacatg ctgaaatatc atctaaatgc tctacaatgc cagcttcttt aaaaataatt
      181 aagccagcat tatatatctg atctctggct tttctggtat aatgccttat tatccctgta
      241 atacgatgtg aattcatagc aattatttat ataggaactc cataaatttc tgcaaagcct
      301 caggtaatgt gccatcttca cgttgatatt ttttaaattt cctttcataa ttgcctactt
      361 cccggttaaa tacattacgg aatctgccgg ctatttcggt atatgcaggc atattttcta
      421 acctatcacg caattccttt ttgaaaggta tatgcctgta aaacatatcc cgtacaacat
      481 tatttagctt catgatacca tctttttcat ataaaaccca ctgcatgtag tcatccgcaa
      541 acctgtcacg gttattgcgg atacttttaa atcgctccac cactttttgc tttgcttcta
      601 atgataactt agaattcttt ttaaatgtat taatataatc aaaatatgct ccagtaagac
      661 caccttctat tggatcggcc cacatacctc ccatgatggt tcgattgagt tcccacctga
      721 acgtagcaaa tgtatgcgcc atggttggca tcatatcacc cataaaaagg ataggcacta
      781 caatacgccc tctggttctg cggtttgttc catcaagctc ctgccataac attgtttttg
      841 atccaaacga tggcaataaa atgaaatacg gaactacttc ttcttttata agttctttgg
      901 tgtttccaag cattacagca gtttcacgat aaaaaacaga aaaatcaaca tcacgcaaag
      961 cacttacaat ggattctaac tttttctttg aaacataata atgtgaaata ctacccttca
     1021 tattcaacga tgtaagtata ggaaacgatg ttgccgttga acctgaacac actgatgttg
     1081 ctgtagcaat acgatgatta atttcataaa ttattttttt tacattatct gttgcatcaa
     1141 tattgctact gccttttata tgtttttgct cctcacgcaa atgagcttca tacgataaac
     1201 ccatctcatt gatgctggga ttttcatcac cattatatat cctggtcaaa aactcatcct
     1261 catatactat tggtgtttgt gtctgctcaa ccctgcgcat tgatatttca tgcagttcat
     1321 tcaattgctc ctgttcaatc attgtttcat caaaaaaacc aaaacgcaac atgagtttca
     1381 caggcggagg aactaccgac tcagtttgac tgcgaacaaa aactttggca aaaatatcat
     1441 ggtaaaaccg tgcgatctgc cttctaactt tacgagcatc tatctcagac gaaagtggat
     1501 ccttcatttt tttaaaatca gacagcagct ttaataaatt tttttgattt tctttttcaa
     1561 tgaaaccaaa ttcaaatatc tgataaacag aatttttata caattgactt atgttttgag
     1621 aaatacgtga tactctttga acttcttcgg caacttttgg ctgatcctgt gtaatttgaa
     1681 tataattatg caatttctta aaactgtcaa attttttaga aagattttcc ccggatattt
     1741 cctgataaaa ctggtaaatt ttgttagcaa ttgacataaa acctttcaag aagccatcac
     1801 ttactcttcc ggaagcaacc catagcttaa atttttgctt atcaattaag tatgcaataa
     1861 atccatcttt cccaaataga tagtccatct caacatcaat ttcattatgt attgattcaa
     1921 tcctgtctaa aagctttaca agattttcag aaatacagtt aaacatatat accgggatag
     1981 tgggatcatt ccgaaacatt gttatcaaca attgattgtc cattgtaaca atctttttta
     2041 agaagttaca ttgttttata tcaacaagat tttcaacagg cagtccagga aatgtatatt
     2101 tcttctttaa taaattggaa ttatcctgaa caataaattt tgcatcaatc ctcgaaggga
     2161 agtcaccgcc attctgtgta tatgttttat aaatatcgtc agcacgttgc gacagtgtat
     2221 ccgtcaggtt gtcggcaagt tcccccgcaa taagggaaaa attatcgtta atacgacata
     2281 ggttttgata taataacgta taccgtttta catccgttaa tgatacttca aaccgtttaa
     2341 taacatgtgt taacaataca gcagcaaaag aagggtcgtc cattactatc tgtctaaaac
     2401 caccgtctcg caaaggatat tttttgacaa tacaatcttc aatagcacga atggtttttt
     2461 tatatggctc tgtaaaaagt aaacttaatc cggctattac cgttt
//
LOCUS       DAOSOX010000286         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_42080_length_2484_cov_9.141622, whole genome shotgun sequence.
ACCESSION   DAOSOX010000286 DAOSOX010000000
VERSION     DAOSOX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42080_length_2484_cov_9.141622"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PLX22_12480"
     CDS             <1..1358
                     /locus_tag="PLX22_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20755.1"
                     /translation="SGGSMDASNILKPVLADGKIRCIGTTTYDEYRKYFEKDGALSRR
                     FQKIDIHEPSHEDTIKILKGIKSRYEEHHNVIYTDDALAQAVELSARYITERKLPDKA
                     IDVIDETGAFARMYARDEENPTITAHQIEQMIAKMARIPERTVSASETAKLQYLGDEL
                     SMHIFGQDMAVQKVIEAIKRSRAGFRETEKPIASFLFVGPTGVGKTELARQLAFIMGI
                     ELIRFDMSEYQEKHTVARLIGAPPGYVGYEEGGQLTEAVRKHPYCVLLLDEIEKAHPD
                     VFNALLQIMDYATLTDNTGRKADFRNTIIIMTSNAGVREMNKKVLGFEVATYNLDAVN
                     KELERLFAPEFRNRLDAIVYFNGLNKDMMRRIVIKKLDEFSKQLQEKHVELVCTDACI
                     DWLSDKAYSPVFGAREVARVIQEHIKSFFVDAVLFGALKNGGRAIADIVGDAISITIE
                     Q"
     gene            1366..2097
                     /gene="aat"
                     /locus_tag="PLX22_12485"
     CDS             1366..2097
                     /gene="aat"
                     /locus_tag="PLX22_12485"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOT20756.1"
                     /translation="MVHFLNNDIICFPPVEEANEDGLLAFGGNLTTEWLLEAYTHGIF
                     PWFNSDRSEILWWSPDPRFVLFPHELKIHRSLKQVMKKKIFTVSFDTAFAQVISECSK
                     PRRQAFGTWITTRMKSAYIKLHELGFAHSVEVWHNGQLAGGLYGVSLGNTFFGESMFT
                     LMDNASKIALVTLVTVLRRLEFALIDCQVYTDNLKHFGARFIPRDEFIRLLTMSPGYT
                     TLCGSWSHLVVDGYDYVQCISEVNA"
     gene            complement(2078..>2484)
                     /locus_tag="PLX22_12490"
     CDS             complement(2078..>2484)
                     /locus_tag="PLX22_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20757.1"
                     /translation="GIVITLLMVKRFVEISQELFTRYSEIFARWMVKQLKGIPMAIGK
                     WVLYGTFSFIFILLVIYAAAVVVSICSLSSLVYFRIIVVAAGVMAVAVISLSLYFVFT
                     VFTNALLHYPKIRYTKIKAKNNHLLESKRLLH"
BASE COUNT          742 a          463 c          585 g          694 t
ORIGIN      
        1 tgagcggcgg ttcaatggat gcttccaata ttttaaaacc ggtgttagcc gatggtaaaa
       61 tccgttgcat aggcaccaca acctatgacg aatatagaaa atattttgaa aaggatggcg
      121 cactttcacg gcgctttcag aagattgata tccatgaacc atcgcatgag gatacaatca
      181 agatcctcaa gggtataaaa tcccgatacg aggagcatca taatgtaatc tatactgatg
      241 atgcactggc acaggcagtt gagctatcag cgcgatatat tactgagcga aagcttcctg
      301 acaaagcgat agatgttata gatgagacag gagcttttgc gcggatgtat gcccgtgatg
      361 aagagaaccc caccatcaca gcccatcaga ttgaacagat gatagctaaa atggcgcgta
      421 ttcctgagcg cacagtttct gcaagtgaga ctgcaaagtt gcagtattta ggggatgagc
      481 tttcaatgca catctttgga caggatatgg cagtccaaaa ggttatagag gccatcaagc
      541 gttcgcgcgc aggcttccgg gaaacagaaa aacccattgc atcgttttta tttgttggtc
      601 caacaggcgt tggcaaaacc gagctggcgc gacagcttgc atttatcatg ggtattgaac
      661 ttattcggtt tgatatgagc gagtatcagg aaaagcatac tgttgcccgg ctcattggag
      721 cgccacccgg ctatgtagga tatgaagaag gaggacagct tactgaagcg gtacgcaaac
      781 atccatactg tgtgttgctg cttgacgaga ttgaaaaagc acatcctgat gtttttaatg
      841 cccttttgca gattatggac tatgcaaccc ttacggataa tacaggacgc aaagctgatt
      901 tccgcaatac aattatcatt atgacatcca atgcaggtgt tcgtgaaatg aataaaaagg
      961 tgcttgggtt tgaagtggct acctataatc ttgatgccgt taacaaagag ctggaacgcc
     1021 tttttgctcc tgagttcaga aacaggctgg atgcaatagt gtattttaat gggctcaaca
     1081 aagatatgat gcgcaggatt gtgattaaaa agcttgatga attttctaaa cagttgcagg
     1141 aaaaacatgt agagcttgtc tgtaccgatg catgtatcga ttggctttca gataaggcat
     1201 attccccggt gtttggagca cgtgaggttg cccgtgttat acaggaacac atcaagtcat
     1261 tttttgtgga tgcagttctt tttggagcgc taaaaaatgg aggcagggca atagctgata
     1321 ttgtcggtga tgcaatatcg ataactatcg aacaatagta catgtatggt gcattttttg
     1381 aataacgata ttatatgttt tcctccggtt gaagaggcaa atgaagatgg attgctggca
     1441 tttggcggca atctaacgac agaatggttg cttgaagcct acacgcatgg catattcccc
     1501 tggtttaata gcgacagatc agaaattttg tggtggagcc ctgatccgcg ttttgtgtta
     1561 tttcctcatg aattgaagat tcacaggtca ctgaagcagg tgatgaagaa aaaaatattt
     1621 acggtgagct ttgatacagc ttttgcacag gttatatcgg aatgttcaaa accgcggagg
     1681 caggcttttg gcacatggat aactacaagg atgaaaagtg catatataaa actgcatgag
     1741 ctgggatttg cacattcggt agaggtgtgg cacaacggtc aacttgcagg agggctttat
     1801 ggagtatcgc tggggaatac tttttttggc gagtcaatgt ttacccttat ggacaatgcc
     1861 tcaaagattg cactggtaac gctggttacc gttctgcgcc gattggaatt tgcgcttatt
     1921 gattgccagg tatatactga taatctaaag cattttggtg cacgattcat accaagggat
     1981 gaatttatac ggcttcttac catgtcgcct ggttacacta cattgtgtgg tagctggagt
     2041 catcttgttg ttgatggcta tgactatgtg cagtgtatca gtgaagtaaa cgcttagatt
     2101 ccagcaggtg attgtttttt gcttttatct ttgtatagcg tatttttgga tagtgtaaca
     2161 atgcattggt aaaaacggta aaaacaaaat ataatgaaag cgagataacc gctacagcca
     2221 taacacctgc tgcaacgaca attatacgaa aatacaccaa tgaagaaagt gaacatatgg
     2281 atactacaac ggcagccgca tagataacca ataatataaa aataaagcta aaggtaccat
     2341 acaaaaccca tttccctatt gccatgggta tcccctttaa ttgtttgacc atccaccgtg
     2401 caaatatttc tgagtacctg gtaaaaagtt cctgtgaaat ttctacaaac cgtttaacca
     2461 ttaatagagt tatgacaata ccag
//
LOCUS       DAOSOX010000287         2483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_42106_length_2483_cov_7.992998, whole genome shotgun sequence.
ACCESSION   DAOSOX010000287 DAOSOX010000000
VERSION     DAOSOX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42106_length_2483_cov_7.992998"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..287
                     /locus_tag="PLX22_12495"
     CDS             9..287
                     /locus_tag="PLX22_12495"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT20758.1"
                     /translation="MNRWNDWFEQGKRDFERANIDIEYEYYEWACFSLQQASEKVLKA
                     LSMKSGLTIWGHSLTEILNILAGRINMPDDITSSAKLLDIYYIPTRLS"
     gene            350..694
                     /locus_tag="PLX22_12500"
     CDS             350..694
                     /locus_tag="PLX22_12500"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOT20759.1"
                     /translation="MRQIVSSGSVKACWVNNEDVINTLKIVAVQAKQKFPEIIEIWLF
                     GSFAKGEATGISDIDIFIVAQTKIDNPLERIKPYYNYFSDCLKIAVDIIVAKPDEKKY
                     HEEMLNDAILLA"
     gene            1279..1575
                     /locus_tag="PLX22_12505"
     CDS             1279..1575
                     /locus_tag="PLX22_12505"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HOT20760.1"
                     /translation="MNDIITEVRVFPKDNLGKTLGFANITLLDKFVVKNLRIVKGDKG
                     IFIGMPSNKRKNGEYIDLFFPITQEARDLITSAIIDKYNELMNTSYPVENKVKQ"
     gene            complement(1771..2085)
                     /locus_tag="PLX22_12510"
     CDS             complement(1771..2085)
                     /locus_tag="PLX22_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20761.1"
                     /translation="MNGDVLIQNIINLFVMAIILEAAVMAIFSMSTFRDMKMTRAIEA
                     SRDVIILLSAFFLCYKVDVLHVFRKTGLNIPVLFDIIISTLVLTRMTNFVRQVFNRLR
                     QD"
BASE COUNT          836 a          407 c          405 g          835 t
ORIGIN      
        1 atctttaaat gaatagatgg aacgactggt ttgaacaggg gaaaagagat tttgaaaggg
       61 ccaacataga tatagaatac gaatactatg aatgggcttg ctttagtttg caacaggcat
      121 ctgaaaaagt tttaaaagca ttatccatga agtccggttt aacgatatgg gggcattcat
      181 taacagagat tctgaatatt ttagctggca gaattaacat gcctgacgat attacctcca
      241 gcgcaaaact tctggatatt tattatattc caacacgatt atcctaatgg atttgcatcc
      301 ggcaaaccgg ctgattattt tacaaaaaaa caggctatgg aggcacatga tgcggcagat
      361 agtatcatca ggttctgtga aagcatgctg ggtaaacaat gaagacgtta ttaatacatt
      421 aaaaattgtt gctgtacagg caaaacaaaa gttccccgaa attattgaaa tctggctatt
      481 tggttcattt gctaagggcg aagccacagg tataagtgat attgatatat tcatagtagc
      541 gcaaaccaaa atagataatc cattggaaag gattaagcca tactataact acttttcaga
      601 ttgcttgaag attgctgttg atattatagt agcaaaaccc gacgagaaga agtatcatga
      661 agaaatgctt aacgatgcca tccttcttgc ataaatgatt atttcataac tatattttgg
      721 atgttcacct gttatcacta aatcaatatt gctacaatgt tttttcaata aaggtaacca
      781 tgattgatta ggttttcata aaataccatg acatcaccaa actgaatatt gaacacttgt
      841 gagcatttaa caacagcgaa attactccct ccatgattga tacaattaat ctgtcatttt
      901 ttattatctt caatttgcca gaaagatcat ggttatttag atcaattaaa aagcaattat
      961 ttagaaatat gtgaatcgtt ttttacatac tatttaaatt tgattaaatt aacaattgca
     1021 attatttttt agataaaaag tggttgtttt acagatacaa tactaatcaa aagtaccaac
     1081 tacccataag tgacacattc ttgtgctatt ttacatggta cttgcttttt acgaaatgca
     1141 taaatgtttc ttttaaacta tgatatcaat taacagtaac tatcgcccaa ttgtttttta
     1201 aaaaattatt ttgcttgact tattataagc cttacgtgta tactctatca acaaaacata
     1261 cggtatggtg gtgatcacat gaatgacatt attaccgaag ttagggtatt tcccaaggat
     1321 aatttaggga aaactctggg gtttgccaat atcactttgt tagacaaatt tgtagtaaaa
     1381 aatctgcgta tagttaaagg tgacaaggga atttttatag ggatgccgtc taacaagcga
     1441 aaaaatggtg agtatattga cctgttcttt cccattactc aggaagcaag ggacctcatc
     1501 acatctgcaa taattgacaa gtacaatgag ttgatgaata ccagctatcc tgtagagaac
     1561 aaagttaaac agtaattgct ataaagcccg cttcggaatc attgcaaaaa gtctttacat
     1621 ctctttatag atgatgtgat ggtttttaat taataaactt gcgttttatc tttttaaacc
     1681 agtagtttta tccatttaaa aatttgaagc gacaggtttt ggtatggtaa gagatgataa
     1741 cgttaccgtg acccggctta cactcttcat ttaatcctgc cgcaaccgat tgaatacctg
     1801 tcgaacaaaa ttggtcatac gtgttaacac taatgtactg ataattatat caaacagcac
     1861 gggtatgttc aaccctgttt tcctgaaaac atgtaataca tcaactttat aacataggaa
     1921 aaaagcagat aataaaatta ttacatccct acttgcttca atagcgcgtg tcattttcat
     1981 atcacgaaat gttgacatcg aaaatattgc cataacggca gcttccagta ttattgccat
     2041 tacaaaaaga ttgataatat tttgaattag cacatcgcca ttcatgtatc aaaacctcct
     2101 gttaattatt tttgtactcc atccatgata attactttgt tttgtataat ataaaattat
     2161 taatgtcaat aatatttttt ttacttacta tcctcacatc actatgtatg atctcctttg
     2221 ttataaaaat tctatcaact ttgattttat gtgttgtacc acatctaaat gttaccatag
     2281 tttgagtagc aataatgctt atagttacac tactaccttt catatgcata tgtttgtgtt
     2341 gctggattga ctatataatt atgtaatgtt aaggtttgtt agtatttccg gcggtggtcc
     2401 ccagtaaaat tggcaacatt gtattccttc ttctaagaaa aacctgaatg tatagcctcc
     2461 acatcgatag ttatcggtat tat
//
LOCUS       DAOSOX010000288         2452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_42853_length_2452_cov_6.699207, whole genome shotgun sequence.
ACCESSION   DAOSOX010000288 DAOSOX010000000
VERSION     DAOSOX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42853_length_2452_cov_6.699207"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLX22_12515"
     CDS             complement(<1..493)
                     /locus_tag="PLX22_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOT20762.1"
                     /translation="MQQIVSFLSKPSSYNHAVDTVHVIQTHISYVFIAKPFVYKVKKP
                     VDFGFLNFVSFEDRKFYTFEEYRLNARISPEIYIGVVVVIKQGASYTLIEDSNYNESD
                     VVAYCVKMRYIDDAYSLKNLLQHGLDDSLLSLIAKKIYTFHSIADNTEGLQGFGGIAE
                     ISKN"
     gene            complement(624..1115)
                     /locus_tag="PLX22_12520"
     CDS             complement(624..1115)
                     /locus_tag="PLX22_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20763.1"
                     /translation="MDFETTTCISCNHLEILASYSEKMDVPLRTLIVYLIMYSAKHEK
                     NQVNAFKRIAYRQRNPDNPWKRLHLVMYYSEYEFFLDVKKLWKMSLAKIIEFCMENVL
                     DEFIQYFSKLIKEINTDNYPDNFPSYYENRSYTFDFHREKGIHCLKFYWGPPPEILGK
                     QNI"
     gene            1333..1911
                     /locus_tag="PLX22_12525"
     CDS             1333..1911
                     /locus_tag="PLX22_12525"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT20764.1"
                     /translation="MIARIDHVSIAVPDFEKAKIFFEALGAIPGAGAKDDATKFFWQI
                     FSLGDMSRIELITPTDKGSFLDGFIQSRGSGVHHITLQTYDIKTAIAKLEQLKIPYFG
                     YNEYPGGVWKEIFIHPKHAFGVLIQIAEFNADDWLSPEVKMPDGQFSITVNNGTVLLT
                     LPHPGGGTATINLTKEQAKKLAHNLLNSIDEK"
     gene            complement(1932..>2452)
                     /locus_tag="PLX22_12530"
     CDS             complement(1932..>2452)
                     /locus_tag="PLX22_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOT20765.1"
                     /translation="VNKAKKIIIVPGYGMALSQAQEQVKLLFDYLITKGKDVRFAIHP
                     VAGRMPGHMNVLLAEVDIPYEYLYDIDTINPEFASTDVVIVVGACDVINPEAITAQGT
                     PIYGMPILRVHEAKHVIVCNKDTKPGYSGVENSLYNKSNVTLLLGNAADTLAELISQL
                     HNDLQSDTVLQN"
BASE COUNT          823 a          437 c          393 g          799 t
ORIGIN      
        1 agtttttgct aatttcagca atgccgccaa atccctgtaa gccttcggtg ttgtccgcta
       61 tactgtgaaa tgtgtaaatt ttttttgcta tgagcgatag taagctgtca tccaatccat
      121 gctgcaataa attcttcagt gaatatgcat catcgatgta tcgcattttt acacaatagg
      181 caacaacatc cgattcatta taattactat cttcaattaa tgtatacgat gctccctgct
      241 ttataacaac aactacgcct atatatattt cagggctgat tcgtgcattt aaacggtatt
      301 cttcaaatgt ataaaatttt ctatcttcaa aagatacaaa atttaaaaaa ccaaaatcaa
      361 caggtttctt taccttatac acaaatggct ttgcaataaa tacatacgat atgtgtgttt
      421 gaataacatg tacagtatca acggcatgat tatacgacga tggctttgaa agaaatgata
      481 caatctgttg catattaacc tcgtacttat agcattgata tatagcacaa tagatgcatg
      541 tatggtagca acaaaataaa gagtacaatc cagtaaattg gcaagaaatt ttttaaaaca
      601 tttttacaaa aaattttacc cgttcatata ttttgctttc ccagtatttc gggcggtggt
      661 ccccagtaga atttgagaca atggatgcct ttttcccggt gaaaatcaaa tgtatatgac
      721 ctattttcgt aataactggg aaaattatca ggatagttat cggtattaat ttctttaata
      781 agcttgctga aatattgaat aaattcatcc agtacgtttt ccatacaaaa ttcaataatc
      841 ttagccaatg acatcttcca cagcttcttt acatctaaga aaaattcata ttctgaataa
      901 tacataacaa gatgcaatct tttccatgga ttatcaggat ttctttgtcg gtaggctata
      961 cgtttgaatg cattaacttg atttttttca tgttttgcag aatacatgat aagataaact
     1021 atcaatgtcc gcagtggcac atccatcttt tctgaatagc ttgccagtat ttctaaatga
     1081 ttgcatgaaa tacatgtagt agtttcaaaa tccattattt cctcccaaat gtttttaatt
     1141 ttaaaaaaat atatcgccat cgtctggact atagcatctc tttatactga gcttaatacg
     1201 ttaccctata acaatgcaat tatttttttg tcgatagtta atttataaat aactatcgct
     1261 taaaaatgat ttgaaatttt tatcaatacc attagtatta ccttttaaca aaacaataac
     1321 gaggttgctt ttatgatagc ccgcatagac catgtatcga ttgctgtacc agattttgaa
     1381 aaagcaaaaa tattttttga agcattaggt gcaatacccg gtgctggagc aaaagatgat
     1441 gctacaaaat ttttctggca gatattttct ctgggtgaca tgagtcgcat tgaacttatc
     1501 acaccaaccg ataagggaag ttttttagat ggattcatac aatcacgagg aagcggtgtg
     1561 caccatataa cattgcaaac gtatgatata aaaactgcta tagcaaaatt ggagcaatta
     1621 aaaatacctt attttggata taacgaatac ccgggtggag tatggaaaga aatttttatt
     1681 catccaaaac atgcttttgg tgtacttatt caaattgcag aatttaatgc tgacgattgg
     1741 ttgtcacctg aagtaaaaat gccggatggg cagttttcaa tcacggtaaa caatggtacg
     1801 gttttactaa cactgccaca tccgggtgga ggtacagcaa caataaacct gacaaaagaa
     1861 caggcaaaaa aattagcaca taatcttctc aacagtattg acgaaaaata ataaactaca
     1921 attcagatac cttaattttg caaaacagta tctgattgta aatcattgtg aagctggctt
     1981 attaattcag ccagcgtatc ggcagcatta cccaacaaaa gagtaacatt acttttattg
     2041 tataatgaat tttctacacc tgaatacccg ggtttagtgt ctttattgca aacaatcaca
     2101 tgttttgctt catgaacacg tagtataggc ataccataaa tgggagttcc ctgggcagta
     2161 atggcttccg gatttattac atcacatgca ccaactacta taacgacatc agttgatgca
     2221 aattcaggat tgatggtatc aatatcatag aggtattcat acgggatatc aacctcggct
     2281 aaaaggacat tcatatgacc tggcatcctg cctgcaacag gatgaatagc aaaacgcaca
     2341 tccttacctt ttgttattaa ataatcaaaa agtaatttta cctgttcctg agcttgtgac
     2401 aatgccatgc catagccagg tactatgata attttttttg ctttattaac ta
//
LOCUS       DAOSOX010000289         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_43559_length_2420_cov_8.715433, whole genome shotgun sequence.
ACCESSION   DAOSOX010000289 DAOSOX010000000
VERSION     DAOSOX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43559_length_2420_cov_8.715433"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLX22_12535"
     CDS             <1..450
                     /locus_tag="PLX22_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT20766.1"
                     /translation="MELSPSAWLTIGIILMAVEILLPGFIIFWFGIGALITAVLVYSG
                     LLESYTWQWFCFFISSGAFLGLWFGYLKDKVKVKTADDSPDVTLTGKKGECTHDIIPP
                     QIGEVELYEPFHGVKIWKAQSSEQIAKGEQIVVEGADGIKLIVKKHE"
     gene            491..1369
                     /locus_tag="PLX22_12540"
     CDS             491..1369
                     /locus_tag="PLX22_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOT20767.1"
                     /translation="MQILIIAVAILIIILIYKGVRIVPQAENWLVERFGKYAATLTPG
                     LNLINPLFSKVAAKIDIREQVLDLPPQGIITEDNAAVKVDGVVFFKILDPYKAFYGIE
                     NLHMAISNLALTTLRSIMGKMTLDQSLSSRDKINAELLIILDQATDSWGTKITRVEIK
                     DIVPPQDLQQAMALQMKAERERRATVLEAEAQKEAQEKKAEGFKRAQILEAEARKESA
                     QRDAEARERLAQAEANAIEFVAKSLKSTGGDPLLYLLGMEYVKGLIKLGESQSSHVVI
                     LPSDLIENLKSLFKLK"
     gene            1555..>2420
                     /gene="mgtE"
                     /locus_tag="PLX22_12545"
     CDS             1555..>2420
                     /gene="mgtE"
                     /locus_tag="PLX22_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOT20768.1"
                     /translation="MMIAPLIQPEIKELIDNREFSVLKEVFSEWAPADLAELISELPE
                     DEQIIIFRLLPKQLQSQTFEYLQYDIQEHLLRNLAKKQIADILNEMSPDDRTALFEEL
                     PADLLRQMLALLSKEERVTAMTLLGYPENSVGRLMTTDYIAVKENMTIAQVLHYIRKY
                     GKDSETLNMIYVIDDNGKLIDDMKMRQVLLSQPTRRIKTLMDHKFISLNAFDDQETAI
                     QVFKKYDHYALPVIDADGALLGIVTIDDVLDVEEEEVTEDIHKMAAVEALEEPYIDEP
                     ILRLTRKRATWLV"
BASE COUNT          795 a          354 c          505 g          766 t
ORIGIN      
        1 atggaactat cgccatctgc atggttaaca attggtatta ttcttatggc agtagagata
       61 ctattgccgg gatttattat tttttggttt ggtattggag cattgattac tgcagtactt
      121 gtttattcag gtttgcttga atcatataca tggcaatggt tttgtttttt tatttcgtcg
      181 ggtgcttttt tagggttatg gtttggatat ttaaaagata aggtaaaagt taagactgcg
      241 gatgattcgc ctgatgtaac tttaacaggt aaaaaagggg aatgtactca tgatattata
      301 cctccgcaga ttggagaggt ggagttgtat gaaccattcc atggcgtaaa gatttggaaa
      361 gctcaatcaa gtgaacaaat tgccaaaggt gaacaaatag ttgttgaagg tgctgatggc
      421 attaagttga tagttaaaaa acatgaataa taaaaaatat ttttatacta aaaatatata
      481 gaggtttact atgcaaatat taattattgc tgtagctata ctcatcatta tccttattta
      541 taaaggagtc aggattgtac ctcaggcaga aaattggctt gtggaacgat ttggaaaata
      601 tgcagctaca ttaacccctg gattaaatct tattaatcca ttgttttcaa aggtagctgc
      661 aaaaatagat attcgtgagc aggtattgga tctgccgccg caaggtataa taaccgaaga
      721 taatgcagca gtaaaagttg atggtgttgt tttttttaaa attcttgatc cttataaagc
      781 gttttatggt attgaaaatt tgcatatggc aatctctaat ctggcgttaa ccacgttacg
      841 ttccatcatg ggtaaaatga ctctggatca atcattatct tcccgtgata aaatcaatgc
      901 agagttgttg attatattgg accaggctac agattcatgg ggaacaaaga taacacgggt
      961 tgaaatcaag gatatagttc ctccacagga tttgcagcag gcaatggctt tgcagatgaa
     1021 agctgagcgt gaacgccgtg caacagtgct tgaggcagaa gcccagaaag aagcgcagga
     1081 aaaaaaggca gaaggattta aacgtgctca gatacttgaa gctgaagcac ggaaagaatc
     1141 agctcagcgt gatgctgaag ctcgtgaacg tttagctcag gcagaagcca atgcaataga
     1201 atttgtagca aaatcattga aaagtaccgg tggagaccca ttattatatc ttcttggtat
     1261 ggaatatgta aaaggattga taaaattggg tgaatcacag agcagtcatg tggttatttt
     1321 accatcggat cttattgaaa atttgaaaag tttatttaaa ctaaaatgaa aaatttatca
     1381 tccgattgtt gatgtgtaaa atgattagca cttaaatatt caaggattta cgttttatat
     1441 atatatcatt ataagcttaa aactctttat tatttattaa aactgtattg atttttttga
     1501 tgcaggattt tcacatttag ttgttaatta ttttttattt ttctgaggca cattatgatg
     1561 attgctcctc ttattcagcc cgaaataaaa gaacttattg ataatagaga atttagtgtt
     1621 cttaaagaag tattcagtga atgggcgcct gctgatttag ctgagctgat aagcgaactg
     1681 cccgaagatg aacagatcat aatttttcgt cttttaccaa aacaacttca atctcagaca
     1741 tttgaatacc tgcaatatga tatacaggaa caccttttac gtaatttagc caaaaagcag
     1801 atagctgata tcttgaatga gatgtcacct gatgaccgaa ctgcattatt tgaagagttg
     1861 cctgccgatc ttttacgcca gatgttagct cttctttcaa aagaagagcg tgttactgca
     1921 atgaccttgc ttgggtatcc cgaaaatagc gttggtcgtt taatgaccac tgattatata
     1981 gctgtcaaag aaaatatgac cattgcacag gttttgcatt atatacgaaa atatggtaaa
     2041 gatagtgaaa ctctcaacat gatatatgta attgatgata atggtaagtt aattgatgat
     2101 atgaaaatga ggcaggtatt gctgtcacaa ccaacacgcc gtataaaaac tttgatggat
     2161 cataaattta taagcctcaa tgcctttgat gaccaggaaa cagcaataca ggtttttaaa
     2221 aaatatgacc attatgctct gccggtaatt gatgcagacg gagcattatt ggggatagtt
     2281 actattgatg acgtattgga tgttgaagaa gaagaggtca cagaagatat tcataagatg
     2341 gctgcagttg aagctcttga agagccatat attgatgaac ctatattacg attaaccagg
     2401 aaaagagcaa catggttggt
//
LOCUS       DAOSOX010000290         2409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_43849_length_2409_cov_12.742566, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000290 DAOSOX010000000
VERSION     DAOSOX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43849_length_2409_cov_12.742566"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLX22_12550"
     CDS             <1..417
                     /locus_tag="PLX22_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20769.1"
                     /translation="GGVLLGSTEKDVMMTRAKSAIKTKSANHDFFKDILKVECQLTLP
                     FNYLYKYNEPECIIGFFNSTVEFIVVWDRRCILIEGVPISQGVMAVVYIYGNYGLACL
                     REDNRSLYIYAEKGIALIDEDNNDTIDGVIIFKTGS"
     gene            complement(465..2243)
                     /locus_tag="PLX22_12555"
     CDS             complement(465..2243)
                     /locus_tag="PLX22_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOT20770.1"
                     /translation="MHQKIDTYITHNTHYIDYCLEQLKKDIYPSLSYISDEIITSLIQ
                     FFLSDGIESPTNLANLSENGLCAAHDLLKLIRQWDIITSGDIIGYIYQSIKNNTDKKK
                     KGQYFTPSAIVQYIVDHTLHYHENLFTIKILDPACGSGQFLTFTYKLLLERYKQFGIH
                     EKEASHLICSNNLFGYDIDPIACSIAQWNMKKISGQKTNIVQRDILDLGTIFTVPHHN
                     EKYDIIIGNPPWGAHFTQTQKNIFKNNYYSAQSGVNSFTLFIERALFLLNESGVLSFL
                     IPEAYLNIKAHKNSRELILNNAIIKEIVLWGDCFKGVYAPAISITAQKESNSKKRLKN
                     VINIKNSNILDSISQMIPQHTYCIAHEHIFSINYSRKAVSILEHIESLNYFTLKEKAK
                     FFLGIVTGNNNHHIAPSQDDNHPHPIIIGKDIEPYKINFSGHYFKYNITTLQQVAEQH
                     FYLNSNKIIYRFISKKLMFAVENNGYYTLNNVNGFIPVDETLDPDIIVTILNSRLMQY
                     IYETQFFTIKVLRGNLEKLPIKKINPANCSILKKFSILLRHEENLLSRKKIMDTIDDI
                     IFHEYNINDIHAHYIYETINSKYSIA"
BASE COUNT          751 a          319 c          444 g          895 t
ORIGIN      
        1 gggggtgttc tgcttggttc aacagaaaaa gatgttatga tgaccagggc aaagtctgct
       61 ataaagacta agagtgccaa tcatgatttt tttaaagata ttctgaaagt ggaatgtcaa
      121 ttaaccttac ccttcaatta tctttataaa tacaatgagc ctgaatgtat cattggattt
      181 ttcaatagta cggttgaatt tattgttgta tgggatcgac gatgtatact catagaaggt
      241 gtcccgatat cacaaggtgt tatggcagtt gtatatatct atggtaatta tgggttagca
      301 tgtttgcgag aagacaatag gtcattgtat atatatgctg aaaaaggtat agctttaatt
      361 gatgaggaca ataatgatac tattgacggt gtcattatat ttaaaactgg cagttaagtc
      421 aatagatgaa tttaatgaga acgataagat tatagtacgc tatactacgc aatggaatat
      481 tttgaattta ttgtttcata gatataatgt gcgtggatat cgtttatatt gtattcatgg
      541 aagattatat catcaattgt atccattatt ttttttctgc ttaataagtt ttcttcatga
      601 cgcagtaata tagaaaattt ttttagtatt gagcaatttg caggattaat ttttttgata
      661 ggcaatttct caagattccc gcgtaaaact tttatggtaa aaaattgtgt ttcataaata
      721 tattgcataa gtcgggagtt cagaattgtt acaataatat cgggatcaag tgtttcatct
      781 acaggtatga atccatttac attgttcaat gtgtaataac cattgttttc aacggcaaac
      841 atgagctttt tactgataaa tctgtagata attttattgc tgttaaggta aaaatgctgt
      901 tcggcaacct gttgcaatgt tgtaatgtta tatttaaaat aatgtccact gaaattgatt
      961 ttatatggtt ctatatcttt gccaatgatt attggatggg ggtggttatc atcctgggag
     1021 ggcgcaatat ggtgattgtt attcccggtt acaatcccta aaaaaaattt agctttttcc
     1081 ttcaatgtaa agtaattcag tgattcgatg tgttcaagta ttgacacagc ttttctggaa
     1141 tagttaatgg aaaatatatg ttcatgagca atacaatacg tatgttgggg tatcatctgg
     1201 gatatagaat ctaatatgtt tgaatttttt atattaataa cattttttaa ccgtttcttt
     1261 gaatttgatt ctttttgtgc tgtaattgat atggcaggag catacactcc tttgaaacaa
     1321 tcaccccata atacaatttc ttttataatt gcattattaa gtataagttc tcttgaattt
     1381 ttatgagctt taatatttag ataagcttca ggaattaaaa atgataaaac ccctgattcg
     1441 ttcaataaaa ataaagcacg ctctataaaa agggtaaaac tgtttacacc gctttgtgct
     1501 gaatagtaat tgttcttaaa tatatttttt tgtgtctggg taaaatgagc accccatggt
     1561 ggattgccta tgatgatgtc atatttttca ttatgatgcg gtacagtaaa tatagtgccc
     1621 agatcaagga tatcacgctg tacaatattg gttttctgcc cgctaatttt tttcatattc
     1681 cattgtgcga tagaacatgc tatgggatct atatcataac caaaaagatt gttactgcat
     1741 attaaatgag atgcctcttt ttcatgtata ccaaattgtt tgtatcgttc cagtaacagt
     1801 ttataggtga atgtaaggaa ttggcctgaa ccacaggctg gatctaaaat ttttatggtg
     1861 aagaggtttt catgatagtg caatgtatga tcaacaatat attgcactat tgctgatggt
     1921 gtaaagtatt gccctttctt ttttttgtca gtattatttt taatactttg atatatatat
     1981 ccaataatat cgcctgatgt tattatgtcc cattgtcgta tcagctttag cagatcatgt
     2041 gctgcacata aaccgttttc agaaagatta gcaagattgg ttggtgattc tatcccatca
     2101 gataaaaaga attgaattaa tgaagtaatt atttcatcgc taatatagga taaagaagga
     2161 tatatatctt tttttagttg ctctaagcaa taatcgatat aatgtgtgtt gtgtgttatg
     2221 taagtatcaa ttttttgatg cataggaact atcggtcata ttaattttat tttcataaaa
     2281 aacgcttata tttacatatc gaatacttgt gataaaacga ttagcatttg tgagggttca
     2341 atgcatgaat tatctatcat gtccaatgtg cttgacattg tggtgcagca tgcacagcag
     2401 aatggggcg
//
LOCUS       DAOSOX010000291         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_45458_length_2347_cov_5.869546, whole genome shotgun sequence.
ACCESSION   DAOSOX010000291 DAOSOX010000000
VERSION     DAOSOX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45458_length_2347_cov_5.869546"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLX22_12560"
     CDS             complement(<1..241)
                     /locus_tag="PLX22_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20771.1"
                     /translation="MAHGERINKRKENKRRHIVDIFRHHTTLSKAEVKRYSGYSMDTV
                     MNSFKILKNSGIIVPTEGKQKEKGRKAFFYTLDHEK"
     gene            587..>2347
                     /locus_tag="PLX22_12565"
     CDS             587..>2347
                     /locus_tag="PLX22_12565"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOT20772.1"
                     /translation="MNTIIAMGTILFVILAILFIIIILLQFDKSRGMGILGRYRNTAS
                     CLSTADITITITMVMVAFVMLGSPGLSTFELHSAYSYEKDALQQEKTGAVIAQKGKAL
                     QQVQPDGSQIDDNSMLKVEHDESSFLVYYKNRCILKHSANNPCIAVGNGTAEYAMSKG
                     SFKIKEKIANKINLKKFTIKENSDTITINFDDTVTIAINAKDNCTISFQCKPDYNRLW
                     LKLEAYKKEHIYGCGEQFSKLDLKNKTIPLWVEEQGVGRGHDLITFLATISHGAGGAW
                     NTTYYPQPTFVSSNNYFVHCNISSYAQFSFNDTNHCLYFWQIPVSIVIGYYDSAVECI
                     KAVGDLVGRQPRLPAWVHDGMWLGVQGGTEVVQSKLQDALDSGVRVSAVWVQDWVGKR
                     ITWFGKQLFWDWRYSQELYPDLPALIKELSHKNIKFLGYINCFLTTDGDLYKEASQKG
                     YLVKDKTGAIRHIVTTEFPAGVIDLTNPQACKWIKDVIKKNMIDLGLNGWMADFGEYL
                     EPDTQLYSGELAEQFHNKYPVQWAKVNYEAIQEAGRLGDIVFFTRAGYTGTSQYSTLI
                     WAGDQLVNWSTNDGLASVIPA"
BASE COUNT          739 a          375 c          467 g          766 t
ORIGIN      
        1 gtttttcatg atctaacgta taaaaaaaag ctttcctgcc tttttctttt tgtttgcctt
       61 cggtaggtac tattatacct gaatttttta agattttaaa tgagttcatg acggtatcca
      121 tggaataacc ggaataacgt ttcacttcag cttttgaaag agtagtgtgg tggcggaata
      181 tatctactat atgtctacgt ttattttctt ttcttttatt tattctttca ccatgagcca
      241 tacggtaacc tcgtatttga ttagtttcgt tatataaatc aatatcgata gtatcatttt
      301 cttaaaaaat aacatataac attattgcaa atatttttta ctttttcaat tattttttgc
      361 aatttaaaat gacgaggatt attaattagg taaagcttta tccattcgta tttcaatatt
      421 ttacggttat acgttgcaaa catttaacaa agaatagact tttttcatta cacagatatt
      481 agtcaataat ataatctttt cttgacataa atattatttt agcataatct tacagttata
      541 ctgaaaatat ttcattgtag caaaatctga ataatcaagg tgcagtatga acacaataat
      601 agcaatggga acgatacttt ttgttatact ggcgattctg tttatcatca ttatattatt
      661 gcaatttgat aaaagtagag gcatgggcat attgggtagg tacagaaata ctgcatcttg
      721 tttatccact gctgacatta ccatcaccat taccatggtg atggttgcat ttgttatgct
      781 gggttcaccg gggctatcaa catttgaact gcatagtgca tattcatacg aaaaagatgc
      841 gttgcagcaa gaaaagacag gcgctgtgat agcgcaaaaa ggtaaagcgt tacaacaagt
      901 gcaacctgac gggtcacaga ttgatgacaa ttccatgcta aaggtggaac atgatgaaag
      961 ctcattttta gtttattata aaaaccgttg tattttgaaa cattctgcta acaatccatg
     1021 tattgctgtt ggcaatggta ctgcagagta tgccatgagt aagggttctt tcaaaataaa
     1081 ggaaaagatt gccaataaaa taaatcttaa aaaatttacc ataaaagaaa attctgatac
     1141 gataaccata aattttgatg ataccgttac cattgcaata aatgcaaaag ataactgtac
     1201 tatttcattt caatgtaaac cagattataa cagattgtgg ttaaagcttg aggcatataa
     1261 aaaagaacac atctatggtt gtggtgaaca attttcaaaa ttggatttaa aaaataaaac
     1321 cataccattg tgggtggaag agcagggagt agggcggggc catgatttga ttaccttttt
     1381 agctactatt tctcatggcg ctggtggtgc atggaataca acctactatc cacaaccaac
     1441 ctttgtttca agtaacaact attttgtgca ttgcaatatt tcgtcctatg cacagttttc
     1501 ttttaacgat accaaccatt gtctctattt ctggcagata ccggtatcta tagttattgg
     1561 ttattatgat tctgcagttg aatgtataaa agcggtaggt gatcttgttg gacgacagcc
     1621 tcgcctccct gcatgggttc atgacggtat gtggcttggc gttcagggcg ggactgaagt
     1681 ggtacaatca aaattgcagg atgctctcga ttcgggtgtc agggtttcgg ctgtatgggt
     1741 gcaggattgg gttggcaaac ggataacatg gtttgggaag cagttattct gggactggcg
     1801 atattcacaa gaattatatc ctgacctgcc ggctcttatt aaggaactat cgcacaaaaa
     1861 tataaaattt ttaggctata taaattgttt tttaaccacg gatggcgatt tatataaaga
     1921 agcatcccaa aaaggatacc ttgtaaagga caaaactggc gcaatacggc atattgtaac
     1981 cacagaattc ccggcagggg taattgatct aaccaatcca caggcatgca aatggattaa
     2041 agatgtaata aagaaaaata tgattgattt gggtttaaac ggctggatgg cagattttgg
     2101 cgaatatctt gaacctgata cgcagcttta ttcaggtgag ttagcagaac aatttcataa
     2161 taaatatccg gtacagtggg caaaagtaaa ttatgaagct atacaggaag ctggtaggtt
     2221 gggtgatatt gtatttttta cgcgcgctgg ttatactggt acttcgcaat acagtacatt
     2281 aatctgggct ggcgatcagc ttgttaactg gagtacaaac gatgggcttg cttcggttat
     2341 accggcg
//
LOCUS       DAOSOX010000292         2334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_45791_length_2334_cov_8.526108, whole genome shotgun sequence.
ACCESSION   DAOSOX010000292 DAOSOX010000000
VERSION     DAOSOX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45791_length_2334_cov_8.526108"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLX22_12570"
     CDS             complement(<1..400)
                     /locus_tag="PLX22_12570"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT20773.1"
                     /translation="MNVKKLLSHIFVLLFFSVSYAQQPIEVYFTNPDSNETVTPIQKA
                     LIHTINSASVSIYAALYELSDPLIIDALCNAKQRGIDVRLVLESDRLSSTVKTKLEAF
                     AIPIRLDNRKGFMHNKFFIFDNKAVWTGSYN"
     gene            complement(390..1319)
                     /locus_tag="PLX22_12575"
     CDS             complement(390..1319)
                     /locus_tag="PLX22_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20774.1"
                     /translation="MSSPITEEKTPKGYVFKINTVKRQYVLFNQKGELIYSDENKTKP
                     FHSMKTIVFIGVHPGDVAIKQPYQLFKIQNASIIIPYMLENNTRHYIFIPSDSYLDLI
                     PCMVEYANDIASFISFVKKGIMPDFIIINSELSNDDSAMIQSLCPSSHVEIAGQSVEP
                     PNNDSKRAVDAGNIDINLLSNNPVFLARVHLRNLDLPRVKQILLDMDLSESDAQYIIA
                     FLNTMLQHSQKEELHKHLEKLNELKHAYEFYYYCIAHDDHNVKQLIEKADKKDLVTFQ
                     TLIQKVKLLFPTKEENLRFVDFENYIWEKNNER"
     gene            1757..>2334
                     /locus_tag="PLX22_12580"
     CDS             1757..>2334
                     /locus_tag="PLX22_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOT20775.1"
                     /translation="MNTVNILTSAYYKGAIHSVSSTSGDNKADNVLNDDIESFWCTNK
                     SHNATAEYIVIDYGLAVAVNVIELFPSPSGKSTFPSDFRLESSVDGSNWKIIKTENRY
                     SLDEDRYRVLLPMNAFQFLKLYIIKPKRIAAKYFSEIGGIKVGIQGASAITASSCSSY
                     EHDPSNLLMNDSAKYWESETSNTPSRQWIEID"
BASE COUNT          767 a          341 c          419 g          807 t
ORIGIN      
        1 tattgtatga ccctgtccag actgctttgt tgtcaaaaat aaagaatttg ttgtgcataa
       61 atcctttgcg gttatccagc cgtatgggta ttgcaaaagc ttccagtttt gtttttactg
      121 tactgctcaa cctgtcagat tccagaacaa gcctgacgtc aatgccacgt tgttttgcgt
      181 tgcataatgc gtcaatgatg agtggatcgg atagttcgta taaagcagca tagattgaca
      241 ccgaagcact gttgatggta tgtatcaagg ctttttgtat tggcgttact gtttcattgc
      301 tgtcaggatt ggtaaaatat acttcaattg gctgctgtgc ataggatacg gaaaaaaaga
      361 gtaatacaaa tatgtgcgat agtaattttt taacgttcat tgtttttttc ccagatgtaa
      421 ttttcgaaat caacaaaccg taaattttct tcctttgttg gaaaaagcaa ttttaccttt
      481 tgtattaatg tctggaatgt gacaagatct tttttatcgg ctttttctat gagttgtttt
      541 acattatggt catcatgagc tatacaataa taataaaatt cataagcatg ttttaattca
      601 ttaagttttt caaggtgctt atgcaactct tctttttgtg aatgctgaag cattgtattt
      661 aaaaatgcga tgatatattg tgcatcagat tctgaaaggt ccatatcaag taaaatctgt
      721 tttacccggg gtaaatccag attacgtaaa tggacacgcg ctaaaaatac aggattattt
      781 gataaaagat taatatcaat attgccagca tctacagcac gttttgaatc attatttggt
      841 ggttcaacag attgacctgc aatttcaaca tgagacgatg gacaaagaga ttgtatcatg
      901 gcactatcgt cattgctcaa ttcactattt ataatgatga agtctggcat aatccccttt
      961 tttacaaaac tgatgaatga tgcaatatca ttagcatatt ctaccataca gggtattaga
     1021 tcaagatagc tatcggatgg aataaagatg tagtgccgtg tattattttc taacatataa
     1081 ggaataatta ttgaggcgtt ttgtatctta aaaagctgat atggttgctt tattgctaca
     1141 tcaccagggt ggacaccaat aaatacaatt gttttcatgg agtggaatgg ttttgttttg
     1201 ttttcatcag aatagatgag ttcgcctttt tgattaaata atacgtattg cctttttaca
     1261 gtatttattt taaaaacata accttttgga gttttttctt cagttattgg tgatgacatg
     1321 gattacacct tctttattga tagtaattgt ttataaattt tcattgaaat tttattaata
     1381 attaaaacat tgtaccttga atattgaaga ctattataca tgttgcttgt aaaccatgtg
     1441 tatacagtat acttaataag aggataccaa aaactttact atgtaatact tttgtgggtg
     1501 caaactattg ataaaactat tttccatttt ttaatcacat tgttaatcag ataaaaaaac
     1561 cactttcaga agagcccaca aaaggtaaaa aagatatagt aacagataaa tagtaattga
     1621 taaaaaatca agcaaatgtt aattctttta tttattctta aaagaaaata tgtgctttta
     1681 aatttagatg attttttaca cattagtaca caagaaggcc aaaagaataa ttttattttc
     1741 tgtgggggag taaacgatga atacggttaa tatattaaca tcagcttatt ataagggagc
     1801 aatacactca gtatccagta catctggtga taataaagca gacaatgttt taaatgacga
     1861 tatagaaagc ttctggtgta ccaataagtc acataatgct acagccgaat atattgttat
     1921 tgattatggt ctggctgtgg ctgtcaatgt catagagttg tttccttctc cgtcaggcaa
     1981 atcaacattc ccatcagatt ttagacttga atcaagtgtt gatggcagca actggaagat
     2041 aataaaaaca gaaaacagat attcgcttga tgaagatagg tatcgtgtat tgctgccaat
     2101 gaatgcattc caatttttga aactgtatat cattaaacca aagcgaatag ctgcaaaata
     2161 tttttcggag atagggggga taaaggttgg tatacaggga gcaagtgcaa ttaccgcaag
     2221 ttcatgttca tcatatgagc atgatccatc aaatttatta atgaatgatt ctgcaaaata
     2281 ttgggaatct gaaacaagca atacaccatc tcgtcagtgg attgaaattg atat
//
LOCUS       DAOSOX010000293         2288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_47057_length_2288_cov_6.902373, whole genome shotgun sequence.
ACCESSION   DAOSOX010000293 DAOSOX010000000
VERSION     DAOSOX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47057_length_2288_cov_6.902373"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLX22_12585"
     CDS             complement(<1..252)
                     /locus_tag="PLX22_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20776.1"
                     /translation="MIQNKHDQAAAIEEVSASIEELSAGNENVKLHTDRQFDRIAILS
                     QIMITASDLIKEMIDLTQKALTRMQDIFKKAQSGEASLAL"
     gene            complement(612..2009)
                     /locus_tag="PLX22_12590"
     CDS             complement(612..2009)
                     /locus_tag="PLX22_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOT20777.1"
                     /translation="MQFFLCPCPHKGVFMSFRKKFLKAVSPFQILVLGYLLITVSGAF
                     LLSIPFASKDGQWHNYVDSLFMAASAISTTGLVVVDTGSFYTLFGQLVLMTIFQIGGI
                     GYMSLLMFVLYLFDIKTSITVQLTAKESLSGPTLRILGKFFLVTVIFTLIFEASGAIV
                     LALYWMQKYNSITAVYYGVFHSISTFCTAGFCLFPDSLMSYKDSIIINGSINFLSLAG
                     GIGFIVLYETFAFVSKRLRGIKVKHVSVHTKVVLLTTFIIVITATLLLLVTEHWDYST
                     SIIQRFVYSSFQVISASTTDGFNTIDIGGMSSASIIIIMLLMFVGASPGSTGGGIKTT
                     TFAIICMYIVSLLKGRSNGVVNIYEREIPTESINKALGIFIMFIIVTMIDLISMSMTE
                     KCNFENILFEIISALGNTGLSTGITSQLSITGKVLLTVTMFIGRVGPITFGFFILGNK
                     KVQIVHYPQEDIFVG"
     regulatory      complement(2025..2162)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          795 a          414 c          332 g          747 t
ORIGIN      
        1 aagagcaagt gatgcctcac cggattgtgc ttttttaaaa atatcctgca ttcttgtgag
       61 cgctttctga gtaagatcta tcatctcttt aataagatca gaagctgtta tcataatctg
      121 tgataaaatg gcaatcctgt caaactgcct atctgtatga agcttaacat tttcattacc
      181 agcagataat tcttctatgg aagccgatac ttcttctatt gctgctgcct gatcgtgttt
      241 attttgtatc attttttatc ctttaagagt gcccggcata ttacatcctt gcattcatag
      301 tttattttta acttttacct tgtaatatta caacaatgta tacgcctaaa tatcagggaa
      361 cctttgatca ttagatttat gatattccat tgtggaaaag taatgaaaaa tgttgtatca
      421 tattttaaca atgttttaat aatgaaaaac gttattaata gagatacttt ttagaaaata
      481 tttttatata atattgtgtg ctcgttttta tcatcaagga aatttttatc tattatggta
      541 ataattatac tataaataat tatcattaca gaataatgtt ttgcccatta tttaaaaaat
      601 atcataaatc tttaaccaac aaatatatcc tcctgcggat agtgaacaat ctgtaccttt
      661 ttattaccaa gaataaaaaa accaaatgtt ataggtccta cccggcctat aaacattgtc
      721 accgttaata aaaccttacc ggtaatactt aattgggagg ttatccctgt tgataatcct
      781 gtatttccta aagctgatat tatttcaaat aaaatgtttt caaaattaca tttttcggtc
      841 atactcattg atatcaaatc aatcatagtt actataataa acattataaa aataccaagc
      901 gctttattaa tagattctgt cgggatttct ctttcataaa tatttacaac tccattgctc
      961 cttcccttca ataaggatac aatatacatg catatgattg caaatgttgt tgtcttaatt
     1021 ccaccgcctg tgctgccagg tgaagcaccc acaaacataa gtaacattat aattataata
     1081 ctggcagaac tcattccacc aatatctatg gtattaaatc catcagttgt cgatgctgat
     1141 attacctgaa atgaagaata gacaaatctt tgtataatgc ttgtactata atcccaatgc
     1201 tcggttacca gtagtaatag tgtagcagtt attacaatga taaaagttgt caataatact
     1261 acttttgtat gaacagatac atgtttaact tttatacccc tcaatcgttt agaaacaaac
     1321 gcaaacgttt catacaatac aataaaacct ataccgcctg caagtgataa aaaatttatt
     1381 gaaccattta ttataatact atctttatag ctcattaaac tgtcagggaa taaacaaaat
     1441 cctgccgtgc agaaagttga aattgaatga aacacgccat aatagacagc agttatacta
     1501 ttatattttt gcatccagta caatgctaaa actattgcgc cacttgcttc aaagattaat
     1561 gtaaatataa cagtaactaa aaaaaatttc cctaatattc taagcgtagg gcccgaaagt
     1621 gattcttttg ctgttagctg tacagttatt gatgtcttta tatcaaaaag atacagtaca
     1681 aacatcaaca acgacatata tcctatcccg ccaatctgga aaatagtcat taaaactaac
     1741 tgtccaaata atgtataaaa actgccggta tctactacaa ccaaaccggt ggtgcttatg
     1801 gcggatgctg ccataaataa cgaatcaaca taattgtgcc attgtccgtc ttttgaagca
     1861 aatgggatgg aaagaaggaa tgcacctgaa actgttatta gcagatatcc caataccagt
     1921 atttgaaaag gtgatactgc tttcaaaaat tttttgcgaa agctcataaa taccccctta
     1981 tgggggcaag gacataaaaa aaactgcaaa aagcagttta acgctcttgc ccttccaaaa
     2041 gcctacgagg ttagctgacg ggcttgggtt tggaagtacc ctatccttcg caaggaatac
     2101 gccccataaa gtggttcccc cgcatccatt gctgctttca tggatctcgg cttattgcag
     2161 ctatttaaaa ttaatgagaa atatactgac aaataatact atcgtcaata gatttataaa
     2221 aattttatag cacttataaa ataaaaaaat aagattgaaa aagaaggaat actccgtact
     2281 atgcccgg
//
LOCUS       DAOSOX010000294         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_47717_length_2265_cov_11.945701, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000294 DAOSOX010000000
VERSION     DAOSOX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47717_length_2265_cov_11.945701"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLX22_12595"
     CDS             complement(<1..905)
                     /locus_tag="PLX22_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT20778.1"
                     /translation="MNFEEYFDNDTIQIENTIMASDLSKIDTAMPSTIHIIPVRYKPI
                     FPGIITPLIIAKPRFMHSIDDALNISRSIGIVVLKDDDVEDVALDDIEMFGTAAKIIK
                     KINLPDESINVLINSVCRFKINKITEEKSNILAKVDYIYDKANPKNVEIKALTRAILS
                     QLKILSESNPLFTEEMKLTMLNVDEPGRIADFVTSILNLEKHEYQNILETINVKTRLE
                     KVLVLLQKELEVLSVQRKIQNRINEKIYKQQKDYFLREQLKIIKQELGVEDDRHHEIE
                     QMKSSAEALHLNHESREKIFEEINRL"
     gene            complement(962..1594)
                     /locus_tag="PLX22_12600"
     CDS             complement(962..1594)
                     /locus_tag="PLX22_12600"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOT20779.1"
                     /translation="MFSEIIKQLPSYFESQNNPVIYLLLFFSAILENLIPPIPGDVVT
                     AFGAFMVGTGKLHFIGVYLSTTLGATTGFTLLFLIGKFLGKEFMLNSKFNFFKYDNIL
                     KAELWVQKYGNWLVLSNRFLPGIRSVISLTAGFSNLPTHTVLLFSLLSASAWNLIIIY
                     LGYSLGNNWFIVQNKISHILSQYSKVLLFAGAIVLLVYIAIKFKKYVAKN"
     gene            1772..>2265
                     /locus_tag="PLX22_12605"
     CDS             1772..>2265
                     /locus_tag="PLX22_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20780.1"
                     /translation="MKKLLCSIFIVMMIAGCTTTIKHKQDEDPFALENQKFLEQTTGQ
                     EAFRVLITPESYKVVQVNFKDKIARIADESGDKYILEEIAQYNQIDEAREGIATVWLF
                     PDSGGIMKIRPTQLTFLVELDNLMIEDLQRWTFSFPGKSVEPTKFDVKYRIVLRKKLS
                     DEEI"
BASE COUNT          743 a          378 c          381 g          763 t
ORIGIN      
        1 agcctgttta tttcctcaaa aatcttttct ctggattcat ggttaagatg caatgcttcc
       61 gctgaagatt tcatctgttc aatttcatga tgcctgtcat cctctactcc caattcctgt
      121 tttatgattt ttaattgttc acgtaaaaaa taatctttct gctgtttgta tatcttctca
      181 ttgatacgat tttgtatttt tcgctgaacg gataaaacct ccagttcttt ttgtaaaaga
      241 accagcactt tttccagacg tgtttttaca ttgattgttt ccagtatatt ttgatattcg
      301 tgtttttcca gatttaagat agatgtcaca aaatctgcaa ttcgtcctgg ttcatcaaca
      361 ttgagcattg tcagcttcat ctcttcagta aacagaggat tgctttcaga taatatttta
      421 agctgactta atattgcgcg tgttaaagct ttgatctcta cattttttgg atttgcctta
      481 tcgtagatat agtcaacttt tgccaggatg ttacttttct cttcagttat tttgttgatt
      541 ttaaacctgc aaacactatt gatgagcaca ttgatgcttt catcaggaag attaattttt
      601 ttaattatct ttgcagctgt gccaaacatc tctatatcat ctaatgcaac atcctcaacg
      661 tcatcatctt ttagtacaac gataccaata gatcttgaga tgtttaatgc atcatcaatt
      721 gagtgcataa accttggttt tgctataata agcggggtaa taataccagg aaatataggt
      781 ttatatcgaa ccggtataat gtgaattgtt gatggcatag cggtatctat tttggaaaga
      841 tcgcttgcca ttatggtatt ttctatctgt atggtgtcgt tgtcaaaata ttcttcaaaa
      901 ttcatttaaa gtctccataa aatcataaac ataaccaggg aaatatagta tatatgcata
      961 actaattttt tgctacatat tttttaaatt taattgcaat atataccaat aaaactatag
     1021 caccggcaaa taacaatact ttgctatatt gtgataagat atgacttatt ttgttttgca
     1081 caataaacca gttattacct aacgaatagc caagatatat aataattaaa ttccatgccg
     1141 aagcgcttaa aagagaaaac agcaaaactg tatgtgtagg taaattagaa aaaccagcag
     1201 ttaaagaaat gacactgcgg atacctggca aaaagcggtt tgaaagcacc agccaatttc
     1261 catatttttg tacccatagt tcagccttaa gtatattgtc atatttaaaa aaattgaatt
     1321 tactgttcag cataaactct ttccctaaaa attttccaat taaaaatagt agcgtaaaac
     1381 ccgtagtagc accaagggtt gttgatagat aaacgcctat aaaatgtaat ttccctgttc
     1441 caaccataaa tgctccaaat gcagtaacaa cgtcacccgg aataggcggt atgaggtttt
     1501 ccagaattgc actgaaaaaa agtaacaaat aaataacagg attattctgc gactcaaagt
     1561 atgaagggag ctgtttaatt atctcactga acatataatt ttacttgaaa ttattgtata
     1621 atacaatata gtttggtgta ttactaaaaa atatttttca tttgtaatga gcgtacacaa
     1681 taggtgatat aataattttt tatacagtga tgtcaagata gtatagttgc tttattttgt
     1741 caagtaagct ggaaagattt atggagatat tatgaaaaag ttactatgtt ccattttcat
     1801 tgtaatgatg atagcaggat gcacaacaac tatcaaacac aaacaagatg aagacccgtt
     1861 tgctcttgaa aatcaaaaat tccttgagca aacaaccggg caggaagcct tcagggtcct
     1921 tataacccct gaaagctaca aggttgttca ggttaacttt aaagataaga ttgcgcgtat
     1981 tgctgatgaa agtggtgata aatatatcct tgaagagata gcacaatata atcaaataga
     2041 tgaagcaagg gagggcattg caacggtttg gcttttcccc gattcaggcg gtatcatgaa
     2101 gatacggcca acgcagttaa cttttctggt ggagcttgac aatttaatga tagaggacct
     2161 ccagcgctgg acattttcat ttccgggcaa aagcgttgaa cctactaaat ttgatgttaa
     2221 atacaggata gttttacgga aaaaactttc cgatgaagag ataat
//
LOCUS       DAOSOX010000295         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_47936_length_2258_cov_6.583296, whole genome shotgun sequence.
ACCESSION   DAOSOX010000295 DAOSOX010000000
VERSION     DAOSOX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47936_length_2258_cov_6.583296"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLX22_12610"
     CDS             <1..626
                     /locus_tag="PLX22_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOT20781.1"
                     /translation="ATDVPFAKQFEMRLAGKFNVYNALAAIGVAHFLNIPAGRIVTGI
                     GLVASVPGRFDIVLSPEGFAVIVDYAHTNDALEKLLLGVRSLNPQRVITVFGCGGNRD
                     KTKRPIMGKVSEEHSDWIIVTSDNPRKENPEDIINNITAGMKSKKYQIIVNREEAIKQ
                     AIAMAESGDAIVIAGKGHEDYQIIGTKKIHFDDKEVALKYINERNAK"
     gene            623..1993
                     /gene="murF"
                     /locus_tag="PLX22_12615"
     CDS             623..1993
                     /gene="murF"
                     /locus_tag="PLX22_12615"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT20782.1"
                     /translation="MIVQFETSSVTIARAIGAKHYGVDMAVKYITTDSRLLDDKSLFV
                     PIAGQKFDGHDFIEEVCKTGKCSSVLTHKHNGVAIADRYGVSAIVCDDTLHAYMDIAR
                     WHRKNFDIPTIGITGTNGKTTTKEMIANVLESQGRVLKTEKNYNNEIGVPYTLLHLNK
                     EHAFAVIEMGMNHLGEIHRLSLAAAPDVAVITNIGEGHLEFVGSTLNVAHAKAEILDG
                     MNQGTCIINNETKHKDIVINKANDKKINVITFGLTGDIRPATFAVYADKTVIQYDGID
                     FSVYAYGFHNVYAILAAIAVAKVLGIPLKHVKESLECFKPVSMRSALHNGRCTIIDDS
                     YNANPLSMEYAFASASMVFPDKRKIAVLSDMKELGNVSKDLHYEIGAKLAHSGFAYLF
                     IWGNDARFYADGAIAAGFERSQVYIYDDKEELINALKQFIQSDDVVLVKGSRSMKMEE
                     VVQALE"
     gene            2004..>2258
                     /gene="mraY"
                     /locus_tag="PLX22_12620"
     CDS             2004..>2258
                     /gene="mraY"
                     /locus_tag="PLX22_12620"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525933.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT20783.1"
                     /translation="MFYHFILPLQEYLSFLRLFQYITFRSAYAALTSLVIVLLFGGVV
                     IRWLTYLRFKEEIRTLGPQSHQKKAGTPTMGGVLVLGSVLI"
BASE COUNT          692 a          372 c          529 g          665 t
ORIGIN      
        1 ttgccaccga tgtgcctttt gcaaaacaat ttgagatgcg tcttgccggg aaatttaacg
       61 tttacaatgc tcttgctgct attggtgttg cacatttttt aaacatacct gcgggcagga
      121 tagtaaccgg tattggtttg gttgcaagtg ttccaggcag atttgatata gtattatcac
      181 cagaaggttt tgcagtgata gtggattatg cacataccaa cgatgcgctg gaaaaattat
      241 tactgggcgt tcgctcatta aatccacagc gtgttattac tgtttttggt tgcggtggca
      301 acagggataa aacaaagcgg cctattatgg gtaaggtaag cgaggagcat tcggactgga
      361 ttatagtgac cagtgataat ccaagaaaag aaaatcctga ggatattatc aacaatataa
      421 ctgctggcat gaaatcaaaa aagtatcaga ttattgtaaa ccgtgaggag gcaattaagc
      481 aggcaatagc tatggctgaa agtggtgatg ctatcgtaat tgcaggcaag gggcatgagg
      541 attatcagat tattggaaca aaaaaaatac actttgatga taaagaggta gcgctaaaat
      601 atatcaatga aaggaatgca aagtgatagt gcagtttgaa acatcttcag taactatcgc
      661 cagagctata ggtgcaaagc actatggtgt tgatatggct gttaaataca tcacaacgga
      721 ttcacggctt ttagacgata aaagtttgtt tgttccgata gctggtcaga aatttgacgg
      781 gcacgatttt attgaagagg tgtgtaaaac tgggaaatgc tcttcggttt tgacacacaa
      841 acacaatggt gttgcgattg ctgataggta tggtgtcagc gccattgttt gtgatgatac
      901 tctgcacgca tatatggata ttgctcgatg gcaccgaaaa aattttgaca ttccaactat
      961 tggtattacg ggaaccaatg ggaagacaac cacaaaagag atgattgcta atgttttgga
     1021 atcgcagggc agggtactga aaacagaaaa aaattataac aacgagattg gtgtgccgta
     1081 tacactgttg catttaaaca aagagcatgc atttgcagtg atagagatgg gaatgaatca
     1141 tttaggtgag atacaccgat tatcactggc tgctgcgcca gatgttgcag taatcaccaa
     1201 cattggagaa ggtcatcttg aatttgtagg atcaaccctg aatgttgcac atgcaaaagc
     1261 agagattttg gatggaatga atcagggtac atgtatcatc aataatgaaa caaagcataa
     1321 ggatattgtc attaataagg ctaacgacaa aaagattaac gttataacat ttgggcttac
     1381 cggtgatata cgccctgcaa cctttgctgt ttatgccgat aaaaccgtga tacagtatga
     1441 tggtattgat tttagtgttt atgcgtatgg attccataat gtttatgcca tactggcagc
     1501 cattgcagtt gcaaaggtat tgggaatacc gctgaaacat gtaaaagaat cccttgaatg
     1561 ttttaagcca gtgtcgatgc gaagtgctct acacaatggt cgctgtacaa ttattgatga
     1621 cagttacaat gcaaatccgc tttctatgga atatgcgttt gccagtgcat caatggtatt
     1681 tcccgataaa cgcaagattg ctgttctttc agatatgaaa gagctgggca atgtttcaaa
     1741 agatctgcac tatgaaattg gcgctaaatt agcacacagt ggatttgcgt acctttttat
     1801 atggggcaac gatgcaaggt tttatgctga tggtgccatt gcagcaggtt ttgaacgttc
     1861 acaggtttat atatatgacg ataaagaaga gctaataaac gctttgaaac agtttattca
     1921 atctgatgat gtggttcttg ttaaaggatc ccgttcaatg aagatggaag aagtagtaca
     1981 agcattagaa taatgaggta tgtatgtttt accattttat tttaccgtta caggaatatc
     2041 tttcattttt acgattgttc cagtatatta ccttccgttc ggcatatgcg gcattgacat
     2101 cgcttgtcat tgtgttgctg tttggtggag tggttatacg ctggctaacc tacctgcgct
     2161 ttaaagaaga gatcagaaca ttaggtcccc agtcacatca aaaaaaagca ggtacgccca
     2221 ccatgggtgg cgtgcttgtt ttaggttcgg ttcttatc
//
LOCUS       DAOSOX010000296         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_48210_length_2247_cov_6.139142, whole genome shotgun sequence.
ACCESSION   DAOSOX010000296 DAOSOX010000000
VERSION     DAOSOX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48210_length_2247_cov_6.139142"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLX22_12625"
     CDS             complement(<1..513)
                     /locus_tag="PLX22_12625"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT20784.1"
                     /translation="MVKPKALIVTGYGINCDEETKFAFALAGADSKIIHINDIIENRS
                     ILKNYHIFAFPGGFSYGDDTGSGKALANKIKNNLMDEMLSFIQRDTLMIGICNGFQVM
                     VNLGIVPALTNSIGSVEVALEHNASFRFQCRWIDLTINSNSNCIFTQGITRLHIPVAH
                     GEGNFIAPHNI"
     gene            complement(522..>2247)
                     /locus_tag="PLX22_12630"
     CDS             complement(522..>2247)
                     /locus_tag="PLX22_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOT20785.1"
                     /translation="PKKINGKNSVHKKAMPGDNIVMVGGRVGRDGIHGATFSSEALTS
                     GSPATAVQIGDPITQKKLSDAIVKEARDMGLYHSITDNGAGGLSCSVSEMAREAGGFI
                     VYLDKVPLKYHGLSPWQIWVSESQERMTLSVPDETLDDFMQLMKKRGVEAWVIGRFTD
                     SGRGIVYYNGEIVFNIDLQFLHEGLPQKKLTGIYEKKLHPFPEFSDSINATDDLCAMV
                     KRLNTASFEFISTQYDHEVQGNSVIKPLQGKGKVNGNASVIRPVLQSKRGVVLSQGVY
                     PSYSEIDAYWMAACSIDTAIRNAVACGCDFDKLALLDNFCWCDSSNPHRLGQLKEAAR
                     ACYDFAVAYNTPFISGKDSMFNDFKGYDEHFNEVFISIPPTLLISSIGIVSDIAYCQT
                     IDFKKPGDLVYIVGITKDELGGSEFLAYKGEQIKGEKYIGDSIPEVDAMLHEKIYRII
                     EKLLRKNLIASSISVERGGLALAVAKSAMSGLVGVTIDLTKVLHENVMRNDTMLYSES
                     QGRIIVSIAPENKEQFEKLCCDIPFARIGRVKDDQYITITGIDGNEIISVSVCQLHAH
                     YKSTFSEF"
BASE COUNT          686 a          508 c          352 g          701 t
ORIGIN      
        1 tatattgtga ggagctataa aattaccttc accatgtgca accggtatat gcagccgggt
       61 aattccctgt gtaaatatgc aatttgaatt ggaattaatg gttaaatcta tccatctgca
      121 ttgaaatcgg aatgatgcat tgtgctctaa cgcaacttca acactaccaa ttgaatttgt
      181 cagagcagga actatcccta aattaaccat cacctgaaaa ccattacaga ttccaatcat
      241 caacgtatca cgctgaataa acgatagcat ttcatccatt aaattatttt taatcttatt
      301 tgccagtgcc ttaccactac ctgtatcatc accatacgaa aagccgcccg gaaatgcaaa
      361 gatgtgataa tttttaagaa tagatctatt ttctataata tcatttatgt ggattatttt
      421 tgaatcagca cctgctaatg caaacgcaaa ctttgtttct tcatcgcagt ttataccgta
      481 tccagttact attaaagctt ttggttttac cattgctgcc tctaaaattc actgaaagtt
      541 gatttgtaat gagcatgcag ttggcacact gaaactgaaa ttatttcatt accatcgatg
      601 cctgttattg ttatatattg atcgtccttc acccgtccta tacgtgcaaa aggtatatcg
      661 cagcatagct tttcaaactg ttccttattt tcaggagcta tcgatactat aatccttccc
      721 tgtgattctg aatataacat ggtatcattt cgcattacat tttcatggag aacttttgta
      781 aggtcgatag ttactcccac aagtccactc attgcggatt tagctacagc taaagccaat
      841 ccaccgcgtt ccacactgat acttgatgca attaaatttt tacgtaacag tttttcaatg
      901 attcgatata ttttttcatg aagcatggca tcaacttcag ggatactatc gccaatatat
      961 ttttcacctt ttatttgttc acccttatat gccaaaaact cactcccccc taactcatct
     1021 tttgtaatgc caactatata caccagatcg cctggttttt taaaatcaat tgtttgacag
     1081 tatgcaatat cagagacaat accaatggat gaaatcaaca aggtaggagg gatagatata
     1141 aacacctcat taaaatgttc atcataccct ttaaagtcat tgaacatgct atccttgccg
     1201 ctgataaacg gcgtgttata tgcaacagca aaatcataac aggcgcgtgc agcctcctta
     1261 agctgcccca acctatgcgg atttgatgaa tcacaccagc aaaaattatc aagcagtgca
     1321 agtttatcaa aatcgcaacc acaggcaaca gcattgcgaa tagcagtatc aattgaacat
     1381 gcagccatcc aatatgcatc tatctcgcta tacgaaggat atacgccttg cgataaaaca
     1441 acaccacgct ttgattgtag cacaggacga atgacactag cattaccgtt aaccttccct
     1501 ttaccctgca atggtttaat aacgctgttt ccctgaactt catgatcata ctgtgttgaa
     1561 ataaattcaa agcttgcagt attcaaacgt ttaaccatag cacacagatc atcggtagca
     1621 tttatactgt cgctaaattc agggaaagga tgcaattttt tttcataaat acctgtcaat
     1681 tttttttgtg gaagcccttc atgtaaaaac tgaagatcaa tattaaaaac tatctcacca
     1741 ttataatata caatacctct gcctgaatca gtaaacctgc caatgaccca tgcttcaaca
     1801 ccacgttttt tcatcaattg cataaaatcg tcaagcgttt catcaggtac cgataatgtc
     1861 atacgctcct gtgattcgga tacccatatc tgccatggcg atagtccatg atattttaac
     1921 ggaactttat caagatacac tataaaacca cctgcttcac gtgccatctc agatacggaa
     1981 catgatagtc ctccagcgcc attgtcggtt attgaatgat acaatcccat atcacgcgcc
     2041 tctttaacaa tggcatccga aagttttttc tgggtaatag gatcgcctat ctgaaccgct
     2101 gtagcagggc tgcccgatgt taatgcttca gaagaaaatg ttgcaccatg aatgccatct
     2161 ctccctaccc ttccacccac catgacgatg ttatcgcctg gcatagcttt tttatgtact
     2221 gaatttttgc cattaatttt ttttggg
//
LOCUS       DAOSOX010000297         2227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_48777_length_2227_cov_6.952578, whole genome shotgun sequence.
ACCESSION   DAOSOX010000297 DAOSOX010000000
VERSION     DAOSOX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48777_length_2227_cov_6.952578"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2227
                     /locus_tag="PLX22_12635"
     CDS             <1..>2227
                     /locus_tag="PLX22_12635"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT20786.1"
                     /translation="IISSLNIFMLPPLITLITSLVIAGIAILKGKLKKENIFFALLCI
                     WYALLTPNFLCHFIIKDVNTILKIERTIHTLYVFIPFIGVMFYHTVLGITRKYLEISC
                     FVVSALFALLVHTPYYITDLLTYPWGHIAKGGIVFQIFGLYAFSILVYAIVISLLKLR
                     QEKNELVRLKIKYILLALNLIGIFTLCNVPAMMGYNFYPLSNLMFIPLVILAYGILRY
                     RLMDIQSVLHITFIWLCISSVIVIPNIVIYTILYPYFTSIDKTVLFVLFTVWFSLNYF
                     YFRKVQPFIDQLFNRRKYDLKKVQSQFINDMGLLKDLHLLLNELSYILKRTLLVKNVE
                     IFIKDDESNNEFYNLSGEKIVIDKKLEEFLLSHQGVIEKKIVISKDEYNDIRQILIPL
                     FEKFSSEYIISLAHNELVGFIMLSERVNLKPLSGDEIELLQNIKTASSIAIANSLMYQ
                     NLNNLKNNLEMLVAQRTEELQKKNDQMEFELKLAKTVQKTLLPAVLPANNQLYAAVYF
                     KPLMEVSGDFFILEQLDETHYLIGVVDVSGHGIPSALLTSMIKTEIENLARTYLSTSQ
                     ICTQLNRRLTGILQESGFYFTLFLGIVDFVNMELTYTNCGHTDIYVITYNDTVYPLST
                     DSVFIGADEHMNYPECIFELNTKDRIVLYTDGIIEIRSQNSQFYGSERFTNILVASRE
                     FSPQDQIDIIIKDLQNYIQSEQGAIRDDMTLCIIEIGEPIAVDNYLKKAVTFYKKKNY
                     D"
BASE COUNT          744 a          340 c          371 g          772 t
ORIGIN      
        1 atatcatttc gtcgcttaac atctttatgc tcccgcctct tataaccctt attaccagtt
       61 tggtcattgc tggtatagct atactgaaag gaaaattaaa aaaggaaaat atattttttg
      121 ctttattatg tatatggtat gcactgctta ctcctaattt tttatgccat tttatcatca
      181 aggatgttaa taccatttta aaaatagaaa gaaccataca tacgctgtat gtatttattc
      241 ccttcatagg ggtaatgttt taccatacag tattgggaat aacaagaaaa tatctggaaa
      301 taagttgttt tgttgtaagc gcgctgtttg ctttgttggt gcacactcct tattatataa
      361 cggatctttt aacctatcca tggggacata ttgcaaaagg gggtatagta ttccaaattt
      421 ttggattata cgctttctca atccttgtat atgctatagt cataagttta ttaaaattaa
      481 gacaggaaaa aaatgaactg gtacgcttaa aaatcaaata tatacttctt gcattaaatt
      541 taataggtat ttttaccctc tgtaatgtcc cggcaatgat ggggtataat ttttacccat
      601 tgagcaattt aatgtttatc cctcttgtaa ttttagcata tggcattttg cggtatcgtt
      661 taatggatat tcaaagtgta ttgcacataa catttatatg gctttgtatt tcttcggtga
      721 tagttattcc caatatagtt atctatacca tattatatcc ttattttaca tcaattgata
      781 aaacggtact ttttgtatta tttacagtat ggttttcact taactatttt tacttcagaa
      841 aggtacaacc atttattgac cagcttttta atagaaggaa gtacgattta aaaaaggtac
      901 aatcgcagtt tatcaatgac atggggttgt taaaagatct ccatctttta ctcaacgagc
      961 tgtcatacat tttaaaacgg acacttctgg ttaaaaatgt tgaaatattt attaaagatg
     1021 acgaaagcaa caatgagttt tacaatcttt caggtgaaaa gattgtaatt gataaaaagc
     1081 ttgaggagtt tttattgagc catcaaggtg tcatagaaaa aaagatagtt ataagcaaag
     1141 atgaatataa tgacataagg caaattttga taccattatt tgaaaaattt tcttctgagt
     1201 atatcatatc gttagcgcat aatgaacttg ttggttttat tatgttatcc gaacgtgtaa
     1261 atctaaaacc actttctggt gatgaaattg aactattgca aaatatcaaa acagcttcct
     1321 caatagcaat agccaattca ttaatgtatc aaaatctaaa taatttaaaa aacaaccttg
     1381 aaatgctggt tgcacagcga accgaagaac ttcaaaagaa aaatgaccag atggaatttg
     1441 aacttaagtt agcaaaaaca gtacaaaaaa cgttattgcc ggcagttctt cctgctaata
     1501 accaattgta tgcggctgtt tattttaaac ctttaatgga agtaagcggc gatttcttta
     1561 ttcttgaaca acttgatgaa acacattatc ttattggtgt tgttgatgtt tcgggccatg
     1621 gcataccatc tgcattgctt acctcaatga taaaaaccga aatagaaaat ttagccagga
     1681 catatttgtc aacatctcaa atttgtacac aattaaatag aaggttgacg ggcattttgc
     1741 aggaaagcgg cttttatttt accttatttt taggcatagt tgattttgta aatatggagc
     1801 ttacttatac caattgtggg cataccgata tttatgttat cacttataat gacacggtat
     1861 atcccttgtc tactgattct gtatttatag gtgcagatga gcatatgaat tatcctgaat
     1921 gtatatttga gctcaataca aaagaccgta tcgtactcta taccgatgga attattgaga
     1981 taagatcaca aaatagccag ttttatggca gtgaacgttt taccaatata cttgtggctt
     2041 caagggaatt ttcgcctcag gatcagattg atattataat caaggatttg caaaactata
     2101 tacaatccga gcagggggct atccgtgatg atatgacact gtgtatcatt gaaattggtg
     2161 agcccatagc tgttgacaat tatttaaaaa aagcagttac attctataaa aagaaaaatt
     2221 atgacaa
//
LOCUS       DAOSOX010000298         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_49082_length_2218_cov_11.695793, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000298 DAOSOX010000000
VERSION     DAOSOX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49082_length_2218_cov_11.695793"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLX22_12640"
     CDS             complement(<1..250)
                     /locus_tag="PLX22_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20787.1"
                     /translation="MRQKFKSFCILFLLVSVVTCASYDYNKQIGEIEKLFYSGQYKEA
                     ANKLLPTINTKSKDQLLYMMECGLILQVAGDYENSNKVF"
     gene            complement(266..838)
                     /locus_tag="PLX22_12645"
     CDS             complement(266..838)
                     /locus_tag="PLX22_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20788.1"
                     /translation="MKKLSLLLIIFIIGFIACGGGVQYKDASKAEGSREWGPKEIKMT
                     VNTMVESMYSFLKDEWKKPAYIEVKQIRNKTSEHIDTKMLADEITTNLIKRRIKFIDQ
                     SLTTDAISEMEKGMTGMVDPESAIPVGELKSPNMYLTGEVRENVRTVGSKTVQYLVVT
                     LKLINLKTQVVEWQDQQEFLKSASKKKITF"
     gene            complement(956..1978)
                     /gene="mnmH"
                     /locus_tag="PLX22_12650"
     CDS             complement(956..1978)
                     /gene="mnmH"
                     /locus_tag="PLX22_12650"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOT20789.1"
                     /translation="MQIPSITFFDSCSITDKVYVDVRAPVEYTNDHIPDAVNYPLLDD
                     EQREYIGTLYHTRGRDEALMEGIEIVSPKLKPMVSFFSLLSQSSSTIIVYCFRGGMRS
                     ESVVSFLNSIGFNLFKLEGGYKAYRQFIRNYLTNYCFTIPVVTVYGLAGSGKTHILHH
                     NPYSLDLEGCAGNRSSLFGSLGMPDHSQKYFESRIVHILQTINVWPYCLLEGESRKIG
                     NCHIPPIILEAMQKGIALWLDTPIDVRIQHIYQEYMPYITEEAIIAIVDKLQPALGKT
                     KTQYCKQLFCHGKYLEFIQFLLQHHYDPEYQHSIKNISFTASINYTEPHDTANTIDAL
                     IHSMLQ"
     gene            complement(1993..>2218)
                     /locus_tag="PLX22_12655"
     CDS             complement(1993..>2218)
                     /locus_tag="PLX22_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20790.1"
                     /translation="AVYAIPVDYCKAIFTDDYPMEVEAYNGYQLLTTFVSKNNFHPLL
                     FAPSMSHYMDVLFTAIEQLLQFGTVTIRMS"
BASE COUNT          664 a          405 c          404 g          745 t
ORIGIN      
        1 taaaaacttt gttgctgttt tcataatcgc ctgcaacctg caatatcaaa ccgcattcca
       61 tcatgtataa aagctgatct tttgattttg tatttattgt agggagcagt ttattggctg
      121 cctccttata ttgaccggag taaaataatt tttcaatttc gccaatttgt ttgttatagt
      181 cataactggc acatgttact actgatacca gtagaaataa aatacaaaaa gatttaaact
      241 tttgcctcat acaaattact ccggattaaa atgttatttt ctttttactg gctgatttta
      301 aaaattcctg ttgatcctgc cactcaacca cctgcgtttt cagattaata agctttaagg
      361 taactactaa atattgaact gttttacttc ctacagtgcg cacattttcc cttacctcac
      421 cggtcaaata catgtttggt gattttaatt cgccaaccgg gatagcgctt tcagggtcaa
      481 ccatgccggt catacccttt tccatttctg aaattgcatc agtagtaagc gattggtcta
      541 taaattttat gcggcgttta ataaggtttg tcgttatttc atcagcaagc attttggtat
      601 caatgtgctc agatgtttta ttacggattt gttttacttc aatgtaggca ggttttttcc
      661 attcatcttt taaaaatgaa tacattgact caaccatcgt attaactgtc atcttaattt
      721 cttttggtcc ccattcacgt gagccctcag ctttacttgc atctttatat tgcacgccac
      781 cgccacaggc tataaaacca ataataaaaa ttatcaatag caatgaaagc tttttcataa
      841 atataacctc ctgaattatt ataaagatta tggcttgttt tgcataacaa aaagaaacta
      901 atgaaataat cactgctata gataggcagt aaagtcaaga aaaaaatggt ggttattatt
      961 gtagcatgga atgtatcaaa gcatctattg tatttgcagt atcatgtggt tcagtgtagt
     1021 taatagatgc agtaaatgat atatttttga tactgtgctg gtattcagga tcataatggt
     1081 gctgtaataa aaattgtata aattctaaat acttaccatg gcagaacaac tgtttgcaat
     1141 actgcgtttt tgttttaccc agagctggtt gtaatttgtc cactatagca atgatagctt
     1201 cttcagtgat atatggcata tattcctgat agatatgttg tattctgaca tcaatgggag
     1261 tatcaagcca taatgctatc cccttttgca tggcttccag aattattggc ggtatgtggc
     1321 agttcccaat tttgcgtgat tcgccctcaa gcaagcagta gggccatacg ttaatggttt
     1381 gcaatatatg aactatcctg ctttcaaaat atttttgtga atgatctggc atccccagac
     1441 tgccaaataa cgagcttcgg ttgcctgcac atccctcaag gtcaagagaa tagggattgt
     1501 ggtggagtat atgtgtttta ccggagccag caagcccata aactgtaact acagggatgg
     1561 taaaacaata gtttgttaga tagttcctta taaactggcg ataggcttta tatccacctt
     1621 ccagtttaaa gagattaaat ccaatactgt ttaaaaatga tactactgat tcggagcgca
     1681 ttcctcccct gaaacagtaa acaataatgg tggaactgga ttgtgataat aatgaaaaaa
     1741 atgaaaccat aggcttcaat tttggtgaaa cgatttctat cccttccatg agcgcttcat
     1801 ctctgcctct ggtatgatat aacgtgccaa tatattcacg ctgttcatca tcaagaagcg
     1861 ggtagttgac tgcatcaggg atatgatcgt tagtgtattc aacaggtgct cgtacatcca
     1921 cgtatacttt atcagttatg gaacaactat cgaaaaatgt gatagatggt atttgcatag
     1981 gtttttatcg ttttatgaca tgcgtatggt tactgtgcca aactgcagca attgttcgat
     2041 ggctgtgaac agtacatcca tataatgact catggatggt gcaaacagca atgggtgaaa
     2101 attgttcttt gaaacaaaag tcgtcaatag ctgatatcca ttatatgctt ctacttccat
     2161 gggatagtca tcggtaaata tagctttgca ataatctaca ggtatagcat agaccgcg
//
LOCUS       DAOSOX010000299         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_49238_length_2212_cov_7.690311, whole genome shotgun sequence.
ACCESSION   DAOSOX010000299 DAOSOX010000000
VERSION     DAOSOX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49238_length_2212_cov_7.690311"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..660
                     /locus_tag="PLX22_12660"
     CDS             202..660
                     /locus_tag="PLX22_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20791.1"
                     /translation="MDNEDAKGGFLGKLTVSLVLKVICVALVIIVALWLIFNIQDYGK
                     ANLIISNQEITSNDQSSVSKFSNNDKVYFFVDRIMKSKLDCDMVTIALYYFENDSYTQ
                     YKQITFEVDKDFPKLSTYIPGEYFKRHGQYQIKVLLDGKVVATKEFKFGE"
     gene            681..1784
                     /locus_tag="PLX22_12665"
     CDS             681..1784
                     /locus_tag="PLX22_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20792.1"
                     /translation="MRNSIIVLAIIIMCLPDSSFADQLTADSAIRDNKEFIEFMDVPI
                     SNFGQKYKDQFKDIYEIHFNAEVAYLQSDYNRAFKNVYESQKKQVNLYSLLVKDYLDD
                     TRQFLDSISPEVIKSKNKKARLYLNLAYRDREVARTHHTVADASNPKLCSNKIYKYIE
                     AIKMVRRARRYGFLAIFESQNDETKKKIYNHLFEIEREKGNVFYVRFISKTEQQIIDE
                     MQKEYYDVQLPDITQQESKDEETYGKRVEKRVRFKDERRVAEYLLTAEFEKAEPIIRD
                     YVKDFNFNLIVATIEYLIANKSPIVAGYSVEALKRYHDDDYSRYFNPSTIDSLAGEIK
                     VVDTIPKKTGTETTEQQKEQTDKKEEVTAEQGK"
     gene            1854..2105
                     /locus_tag="PLX22_12670"
     CDS             1854..2105
                     /locus_tag="PLX22_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20793.1"
                     /translation="MDNSELLNSIHRRMMNELLNHSNEKPGGQKLKEIVTIDQNLRKG
                     IADLYVQLSELGDKEMAINILSDHVAIMVEMIVHFKSEK"
BASE COUNT          813 a          290 c          436 g          673 t
ORIGIN      
        1 ggtataatac atatcatatt ataaagagta ttgcatcttt gtaatataat tttgaaaaca
       61 ttgcagaaat aacagcagca atttatttat aagctaattg gtagctcaac taaatgtaag
      121 taaatccgct ctgaaacctt tataaaaaga gaggtttgaa ggcggggaat aattctatgt
      181 taagggaggt ccattatata tatggataat gaagatgcaa aaggtggttt tctcggtaaa
      241 ttaaccgttt cattagtatt aaaagttata tgtgttgcat tggtaataat tgttgcactg
      301 tggctaatat tcaatattca ggattatgga aaggcaaatt taattatttc caatcaggaa
      361 attacctcaa acgatcagtc atcggtatca aaatttagca ataatgataa ggtatatttt
      421 tttgtcgata gaattatgaa atcaaaactt gactgtgaca tggtaactat cgccctgtac
      481 tattttgaaa atgatagtta tactcagtat aaacagatta catttgaagt tgataaggat
      541 tttcctaaat taagcaccta tatccctgga gaatatttta aacgacatgg acaataccaa
      601 ataaaagtgt tgctggatgg caaggttgta gcaacaaaag agtttaagtt tggagaataa
      661 ttgttatctg aaggtgaatg atgagaaaca gtataatagt tttagcaatt attatcatgt
      721 gtttgcctga tagtagtttt gctgatcagc ttactgctga tagtgctatt cgggataata
      781 aagaatttat tgagtttatg gatgttccca tatctaattt tggtcaaaaa tataaagatc
      841 agttcaaaga tatttatgag atacatttta atgcagaggt tgcctatctg caatcagatt
      901 ataacagagc gtttaaaaac gtttatgaat cgcaaaaaaa acaggttaat ctctattctt
      961 tgctggttaa agattatctg gatgatacca gacaattttt agattctatc tctccggagg
     1021 taataaaatc aaagaataaa aaagcacgcc tttatttaaa tctggcgtac agagacaggg
     1081 aagtagccag gacccatcat acagttgcag atgcttccaa cccaaagctg tgctcaaata
     1141 aaatttataa atatattgaa gcaattaaga tggtacggag agctcgccga tatggctttt
     1201 tagcaatctt tgaatcgcaa aacgatgaaa caaaaaagaa gatatataat catctttttg
     1261 agattgaacg tgaaaaagga aatgtatttt atgtacggtt tatttcaaaa actgagcagc
     1321 aaataattga tgaaatgcag aaagagtatt atgatgtgca attacccgat ataacgcagc
     1381 aagaatcaaa agacgaggaa acgtatggaa aacgtgttga aaaacgagtg cgttttaagg
     1441 atgagcgtcg ggtggcagaa taccttttaa ctgcagagtt tgagaaagct gaacctataa
     1501 tacgtgatta tgtaaaggat tttaatttta atttgattgt ggcaactata gaatatttga
     1561 tcgcaaataa atctcctatt gtagcaggtt attcagttga ggcattgaaa cgatatcatg
     1621 atgatgatta ttcacgttat tttaatccat ccactattga ttctttagct ggtgaaataa
     1681 aggttgttga cacaatacca aagaaaacgg gaacagaaac aactgaacag caaaaagaac
     1741 aaacagataa gaaagaagag gtaacagccg aacagggtaa gtgatatagc ataaatgcat
     1801 tgtttattgg ctaacgttat acattaattt aatatgaaca ggaagggatc gtcatggata
     1861 attcagaact actcaactca atccacaggc gtatgatgaa tgagctatta aatcactcaa
     1921 atgaaaaacc aggtgggcag aaattaaaag aaatagttac cattgaccag aacttaagaa
     1981 aggggatagc tgacctgtat gttcaacttt cagagctggg tgataaagag atggcaataa
     2041 atattttgtc tgatcatgtt gccattatgg ttgagatgat agttcatttc aaaagtgaaa
     2101 aataagttac gacagatatg ttaaaatgag ggaataacat gaagcaactt tttatgatag
     2161 ttttgtgcat aacaatgttt tcctgttctg aagagcaatt tggaggatta gg
//
LOCUS       DAOSOX010000300         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_49587_length_2201_cov_5.040541, whole genome shotgun sequence.
ACCESSION   DAOSOX010000300 DAOSOX010000000
VERSION     DAOSOX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49587_length_2201_cov_5.040541"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /gene="metK"
                     /locus_tag="PLX22_12675"
     CDS             <1..1145
                     /gene="metK"
                     /locus_tag="PLX22_12675"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT20794.1"
                     /translation="DYLFTSESVSEGHPDKVADQISDAILDEHIKGDPYSRVACETLV
                     TTNRVIISGEITSSVTVDYEAVARKVIASIGYTSPEIGFDAKACNVEIYIHPQSPDIS
                     QGVTEGQGLFKEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLADIRKKGEINF
                     LRPDGKSQVTVEYQNDKPKRISTIIVSTQHDAAVTLPTLEEMVRELVIKNVIPSNLWD
                     KNTKIYVNPTGRFEIGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDRSAA
                     YMGRCIAKTIVAAGLASRCELQVAYAIGVADPVSVMVDTFGTNNIPEETIEKRIQHVF
                     NLKPKSIIQTLDLLKPKYTITAAYGHFGRNEPGFTWEQTDKAQELR"
     gene            1373..2113
                     /locus_tag="PLX22_12680"
     CDS             1373..2113
                     /locus_tag="PLX22_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT20795.1"
                     /translation="MVIRNIQAKSILSVSKVYDYVVNPYVGCQFGCLYCYARYMKKFT
                     GHKEPWGTFVDSKDNAAVLLQKEIAKKKKGQVWISGVCDPYQPLEKKYAITRACLTIL
                     IENDWPVIIQTKSNLVLRDVDIIKNAASCDVGLSITTADDSIRKLFEPKSTAIADRID
                     AIKQLKKHGIRTYVMIAPMLPGCENLIDMLAGSVDYVYVDKMNYHYADSVYKKYALEQ
                     YCDNSYFFSASHEIAYRCKKEGIACSIV"
BASE COUNT          716 a          361 c          493 g          631 t
ORIGIN      
        1 aagactattt atttacttct gaatcagttt cagaaggaca tcctgataag gttgcagacc
       61 agatatccga tgcaatttta gatgaacata taaaaggtga tccgtattct cgtgttgcat
      121 gcgaaacgct tgtaaccaca aaccgggtta taatatccgg tgagattaca tcatcggtaa
      181 ctgttgatta tgaagctgtt gcccgtaagg taattgccag tattggatat acttcaccgg
      241 agattggctt tgatgcaaaa gcatgcaatg tagaaatata tatacatcct caatcacctg
      301 atatttcaca gggtgttacc gaaggacagg ggcttttcaa agagcagggt gctggcgacc
      361 aggggatgat gtttggctat gcagtagctg aaaccgatga gcttatgccc atgcctattt
      421 tatatgcaca caggctggta aaacgccttg cagatatcag aaaaaaaggt gagataaatt
      481 ttttacgccc tgacggcaaa tctcaggtaa ccgttgaata tcaaaatgac aaaccaaaac
      541 gtataagcac tatcattgtt tctacacagc atgatgctgc tgttacgctg ccaacacttg
      601 aagagatggt gcgtgagctt gttattaaaa atgttatacc ttcgaacctc tgggataaaa
      661 acacaaaaat ttatgtcaac ccaacggggc gttttgagat tggcggtccc catggtgata
      721 ccggattaac cggcaggaag attattgtcg atagttacgg tggaatgggc agacatggtg
      781 gcggagcatt ttcaggcaag gatccgtcaa aggttgaccg ctctgccgcc tatatgggac
      841 gctgcattgc aaaaactatt gttgcagcag ggcttgcatc gcgatgtgaa cttcaggtgg
      901 catatgccat aggggttgct gatcctgttt ctgttatggt tgataccttt ggtacaaaca
      961 acataccaga agaaacaatt gaaaaacgca tacaacatgt cttcaattta aaaccaaaat
     1021 caatcataca aacgcttgat ctgttaaaac caaagtatac aataactgca gcgtacggac
     1081 attttgggcg caatgaacct ggttttacat gggaacaaac tgataaagcc caggaattgc
     1141 gatgatgcaa tctgtttcta taaaacaatg atacaacaaa agcgtggatt tccacgcttt
     1201 ttttattgag actttaaaca ttgatatggt acaattttat tgtgatagtg gtattttttt
     1261 gatacagggg taatgggtta tagatatagt gctgttcaaa ataacgatga ttgcaggtgt
     1321 gtaataataa tgatgagtgc catcagaaat tacattatta ttgagcaata gtatggttat
     1381 acgcaacata caggcaaaaa gtatattgtc ggtttcaaag gtatatgatt atgtggtaaa
     1441 tccctatgtg ggatgccaat ttggttgttt atattgctat gcccgctata tgaaaaagtt
     1501 taccgggcat aaggagccgt ggggaacatt tgttgatagt aaagacaatg cagcagtgtt
     1561 gttgcaaaag gaaattgcaa agaagaaaaa ggggcaggta tggataagcg gggtttgtga
     1621 tccctaccag ccattagaaa aaaaatatgc aataacacga gcatgcttaa cgatacttat
     1681 tgaaaatgat tggcctgtca taatacagac aaaatcaaat cttgtgctga gagatgtgga
     1741 tattattaag aatgcagctt catgtgatgt tggattatcc ataacaactg ctgatgatag
     1801 tattcggaaa ctttttgaac cgaaatcaac tgcaatagca gacaggatag atgcaataaa
     1861 acagttgaaa aaacatggca tacgaacgta cgtcatgatt gcacctatgt tgccgggttg
     1921 cgaaaatctt attgatatgc ttgctggtag cgttgattat gtatatgtag ataaaatgaa
     1981 ttatcattat gcagatagtg tgtataaaaa atatgcgctg gaacagtatt gtgataacag
     2041 ctattttttt tcagcaagtc atgagatagc gtatcgctgt aaaaaagagg ggattgcatg
     2101 ttctattgtc taacatgtat tgattgacat ttccatagca ttactctata atataataga
     2161 tgcagatgaa acaatggggg tttgcaatga aaaagataat c
//
LOCUS       DAOSOX010000301         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_50113_length_2184_cov_10.729450, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000301 DAOSOX010000000
VERSION     DAOSOX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50113_length_2184_cov_10.729450"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLX22_12685"
     CDS             complement(<1..788)
                     /locus_tag="PLX22_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT20796.1"
                     /translation="MDHHHEHDSHEYNQGRFILVVVLNVIITAAEYIGGIISGSLALV
                     SDAGHNLSDVISLILGYIGQKVSQHKPGKQYSFGLKRFEVLIALVNALALFVIGGYII
                     VEAVQRYVHAEPINPGVMLPVACIGLAGNVISMLVLYKSRDENLNLKAAFLHLLYDAV
                     SSIGVIITGLVLMVNNSWVILDVIVSVAIALMIVASSLDIIKSSVRIFLQGVPSRLNF
                     DDVYKALLTIPGIASVHGLHIWSVDSNEVFLSCHLCIDKGDSYS"
     gene            972..1580
                     /locus_tag="PLX22_12690"
     CDS             972..1580
                     /locus_tag="PLX22_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20797.1"
                     /translation="MKTAICAAIICSVMVFPAYSQNKNAKPQTAKEEKTTQPKQAKNI
                     AQGYGNVKWGSYLNDVKNNVAGRIIYMDEKRIIISRDGDLEYRYGFFYPEYNQTATEP
                     QQPKLFYVIIQFPYIALDDVKKKMEEKYGPATGESIKNNQGAYIWDSESTAVVLWVDS
                     YEKKPFCRKITYISKIMAKDINEYYATVFSKKEQEIIKKLQP"
     gene            complement(1582..>2184)
                     /locus_tag="PLX22_12695"
     CDS             complement(1582..>2184)
                     /locus_tag="PLX22_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20798.1"
                     /translation="MGLSRFGAGGVFVSEHNYSLVADGYGTIAHYDDTVRLVGNGKAL
                     YVNGQSYFNGTVSLNSDNKEYYASIVEMFKTDDNEYISPGDVLVVSEQGNAILSRSRS
                     AFNKGVIGVVAGNPVMVINNASSEKKLYPVALTGSVLCKVDARQKGVKPGDLLVTSDT
                     PGCAMPALIDSFDKIGTVFAKALSALDDEIALIPVMIWKM"
BASE COUNT          726 a          485 c          378 g          595 t
ORIGIN      
        1 ttagagtaac tatcgccctt atcaatacac aggtgacacg ataaaaacac ctcattggaa
       61 tctaccgacc atatatgcaa tccatgcaca ctggcaatac ccggtattgt taaaagcgct
      121 ttgtatacat catcaaaatt aagtcttgac ggtactccct gcaaaaatat ccgtactgaa
      181 cttttaataa tatcaagaga gcttgccaca atcatgagag ctatcgccac gcttactatt
      241 acgtcaagga taacccagct attgttaacc atgagcacca ggccggtaat gataacccca
      301 attgatgaaa ccgcatcgta cagcaggtgc agaaatgctg ctttaagatt cagattttca
      361 tctctgcttt tatacagcac aagcatggag atgacattgc ccgccagacc aatacatgct
      421 accggcagca tcactccagg attgatgggc tcagcatgta cataccgctg gacagcctca
      481 acaataatat atccgcctat aacaaacagc gctaatgcgt tgacaagcgc aatgagcacc
      541 tcaaaacgtt taagcccaaa cgaatactgc ttgccgggtt tgtgctgtga taccttctgc
      601 ccaatataac ccagtataag cgagatgaca tcagaaaggt tatgccctgc atcagataca
      661 agggctaagc tgcctgaaat aataccacca atatactcag ccgctgtaat gatgacattg
      721 agcactacca caaggataaa ccttccctgg ttatactcat gactatcatg ctcgtgatgg
      781 tggtccatag ataatccttt tttatatact cttgtaattc aatatactat agccacgtat
      841 tgtcatcaat tttttatttt ttggttgcat atatgtgcca tttttctgtt aaaatactat
      901 ggattatcat gaacatattt ccgatagtat tatgtaagag tgagattatt ctatttttta
      961 aggaaaatgg tatgaaaacg gcaatatgtg ctgcaattat ttgttctgtt atggtttttc
     1021 ctgcatattc acaaaataaa aatgcaaagc cccaaacagc caaagaggaa aaaactactc
     1081 aaccaaagca ggcaaaaaat attgcacagg gatacggcaa cgttaagtgg ggaagttatt
     1141 taaacgatgt aaaaaataat gtagcgggca gaatcattta catggatgaa aaacgcatta
     1201 ttatatcccg tgatggtgat cttgaatacc gctatggatt tttttatccc gaatacaatc
     1261 aaacagctac tgaaccacaa caaccaaaac ttttctatgt tattattcaa ttcccctata
     1321 ttgcccttga tgatgtaaag aaaaagatgg aagaaaaata cggacctgca accggtgaat
     1381 ccataaaaaa taatcagggc gcctatatat gggactctga atcaacggct gttgttttat
     1441 gggtagacag ctatgaaaaa aaaccgttct gccgtaagat aacctatata agcaaaatta
     1501 tggcaaaaga tatcaacgaa tattacgcta cagtcttcag caaaaaggag caggaaatca
     1561 tcaagaagct acagccataa tttacatctt ccatatcatc acaggaataa gagctatttc
     1621 atcatcaagt gcactgagtg cttttgcaaa caccgttcca atcttatcaa atgaatcaat
     1681 aagtgctggc attgcacatc caggcgtgtc cgaagttact aaaaggtcac cgggttttac
     1741 ccctttttga cgtgcatcaa ctttgcacaa cactgagcct gttaaagcaa cgggatatag
     1801 ctttttctct gagctggcat tattgataac cataaccgga ttaccagcaa ccacaccaat
     1861 cactccttta ttaaacgcac ttcgtgagcg tgaaagaatt gcgttcccct gttcactgac
     1921 aacaaggaca tcaccaggtg aaatatattc attatcatcg gttttaaaca tctcaacaat
     1981 gctggcataa tattctttat tatcgctatt caggctaacc gtaccgttaa aatatgattg
     2041 cccgttaaca tataaagcct tcccattacc aacaaggcgt acagtatcat cataatgggc
     2101 gatagttcca tagccatcag ccaccagtga atagttatgc tcacttacaa acacacctcc
     2161 cgcaccaaat cgcgataaac ccat
//
LOCUS       DAOSOX010000302         2178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_50321_length_2178_cov_9.267546, whole genome shotgun sequence.
ACCESSION   DAOSOX010000302 DAOSOX010000000
VERSION     DAOSOX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50321_length_2178_cov_9.267546"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="dnaN"
                     /locus_tag="PLX22_12700"
     CDS             complement(<1..681)
                     /gene="dnaN"
                     /locus_tag="PLX22_12700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT20799.1"
                     /translation="MLIEVDKTQLIKATTIADSVVSSKSINTLLTQCLFSITKDTLVI
                     TGTDNEIGIKTDIDVVADESGSFTLSGKRLVNLLKEFPDGIVVLEVNGYNVSISSKSG
                     LVKGQYVLVGASAEEYPVIRDFSYKNAIEIEPKTLYEMLSKTVHAAANDSVKPIFNGV
                     YVSSETEKSLTVVATDSRRLSMMRKDTKGNVTLKEGVIIPLKTVNELLHILNEHDTCL
                     IEFDESRCH"
     gene            complement(941..>2178)
                     /gene="dnaA"
                     /locus_tag="PLX22_12705"
     CDS             complement(941..>2178)
                     /gene="dnaA"
                     /locus_tag="PLX22_12705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT20800.1"
                     /translation="AFNEDVLVIKVVDDVAKRHISDHYTSLILSIIKEQTGKDIICEF
                     VTGNGFKDHIEETSSQKLHEQKETLLFPTILNPKYTFENFVIGPNNQLAHAAAISVSK
                     APATQYNPLFIYGGTGLGKTHLMQAIGHRIIQERPYLNVLYVPCEQFINEFIQAIIAN
                     TISSFKIKYRNVDILLIDDIQFIEKKEQTQEEFFHTFNTLHNNKKQIIISSDRPPKDL
                     STLENRLRTRFEWGMIVDIQVPNLETREAILRNKAEKIKIELSDEVCNYIARRIKSSI
                     RALEAALVRLSMVSNIYNHPITLQLAKDHCKDLFDVDSSKKISINDIMVKVASKFNVT
                     VDELTSKNRQSKVVQPRFIAMYLSRRLTDLTTIEIGKEFGDRDHSTVLNAINNIEKTV
                     NDDVDFKEIVDELISELKS"
BASE COUNT          730 a          361 c          299 g          788 t
ORIGIN      
        1 atggcatcta ctctcatcaa attcaataag acatgtatca tgttcattca agatatgtaa
       61 taactcattt accgttttta agggaattat aacaccttct ttcagggtaa catttccttt
      121 agtatctttt cgcatcatcg atagacgtct ggaatcagtt gctacaaccg tcaaagattt
      181 ttcagtctca gaagaaacat atacaccatt aaaaattggc tttaccgaat cattagctgc
      241 tgcatgaact gttttactga gcatttcata aagagttttt ggttcaattt caatagcatt
      301 tttatagcta aaatctctaa taacgggata ttcttcggca gaagccccaa ccagaacata
      361 ttgtcctttt accaaaccag attttgaaga aattgataca ttatagccat taacttcaag
      421 aacaactata ccatcaggaa attctttcaa taaatttacc aaccttttac cacttagggt
      481 aaatgaacca ctttcatctg caacaacatc aatatccgtt ttaatcccaa tctcattgtc
      541 tgtacctgtt ataacaagag tatcttttgt aatactaaaa agacactgtg ttaatagggt
      601 attaatactt tttgaactaa caacagaatc agctatagta gttgctttaa ttaattgtgt
      661 tttatcaact tctatcaaca ttgttcacac caccaggtat attattacta aatatataat
      721 aaacttatat aaaataagta gtattagtag taaatacttt tttgttaata actatcgaaa
      781 agtctgaaaa tttcagcaat ttcaaaaaaa gtatattgtt aataactatt gattaaacct
      841 ttaatatata gttattaaca gccattgata aaaaaattaa aaaaataaaa caaatattat
      901 gaaaaaacac acattgttgt aacaattaat tcacagtata ttaacttttt aattcagata
      961 ttaattcgtc tactatttct ttaaaatcta catcatcatt gactgttttt tcaatattgt
     1021 tgatggcatt taaaacagtt gaatgatccc tgtcaccaaa ttctttgcct atttctatgg
     1081 ttgttaaatc tgtcaatcga cgtgataaat acatagcaat aaaacgaggt tgtacaactt
     1141 tgctttgtct gttttttgat gttaattcat caacagttac attaaatttt gatgcaacct
     1201 ttaccattat atcattaata gaaatttttt tagaactatc aacatcaaaa agatctttac
     1261 agtggtcttt tgctaattga agtgttattg gatgattata gatattcgag accatactta
     1321 atctaaccag agcagcttcc agtgctcgta tacttgattt tattctccga gctatataat
     1381 tacaaacttc atctgataat tcaattttta ttttttcagc tttatttctg agtattgctt
     1441 ctcttgtttc taaatttgga acttgaatat caacaatcat accccattca aaacgagttc
     1501 gtaagcgatt ttcaagggta gatagatctt ttggaggtct atcgcttgaa ataattattt
     1561 gttttttatt attatgcagt gtattaaaag tgtggaaaaa ctcctcctga gtttgctcct
     1621 ttttctcaat aaactggata tcgtctatta acaatatatc cacattacgg tatttaattt
     1681 taaagcttga tattgtattg gcgataattg cctgtataaa ttcattaata aattgttcac
     1741 agggaacgta aagaacatta aggtatggac gttcctgaat tatcctgtgc ccaattgctt
     1801 gcatcagatg cgttttacca agaccagtac caccataaat aaataaagga ttatactgcg
     1861 ttgctggtgc tttagaaacc gagatagcag ctgcatgagc taattgatta ttaggcccaa
     1921 taacaaaatt ttcaaatgta tatttgggat ttaaaattgt tgggaatagc agtgtttctt
     1981 tctgctcgtg taatttctgc gacgatgttt cttctatatg atccttaaaa ccatttccgg
     2041 taacaaactc gcatataata tctttgcctg tttgctcttt aatgatagaa agaatgagag
     2101 atgtataatg atcagaaata tggcgtttag caacatcgtc aactactttg attacaagaa
     2161 catcttcatt gaatgcta
//
LOCUS       DAOSOX010000303         2169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_50613_length_2169_cov_7.967360, whole genome shotgun sequence.
ACCESSION   DAOSOX010000303 DAOSOX010000000
VERSION     DAOSOX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50613_length_2169_cov_7.967360"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="PLX22_12710"
     CDS             <1..848
                     /locus_tag="PLX22_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002679788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT20801.1"
                     /translation="VEDKATSDGYSTPYKFTGKELDAETGLYYFGARYYDARVSRWIS
                     ADPALADGKYFPKPNDYDTEHDFCWYLQQDGSKKLAGLGGVFNAVNMDVYHYAGDNPV
                     KLVDPDGNQSLNPLDLGLTDPISFVIDILRYKYETPFNEAKNKITSTSIGFATGFVKG
                     FFGPDFSKLIEKCGKNMNGFYAEEFQAAIDSGELTGMVAGLLNFTAGAYKSGQTMEAG
                     YELGTGIVNKIISDPKTRKEFLLGYAKGFVPSAKPPKFNSIVEAAGWLIGNKSYQKYW
                     EEQNK"
     gene            complement(1509..1778)
                     /locus_tag="PLX22_12715"
     CDS             complement(1509..1778)
                     /locus_tag="PLX22_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HOT20802.1"
                     /translation="MEIAFSSSFKRAFKKKIKNRNEIEDLFWEAVSLFIQDPFHSSLK
                     THKLSGKLKNLWSFSVEYDMRVIFFFEENNSKAIFIDIGSHEEVY"
     gene            complement(1781..1990)
                     /locus_tag="PLX22_12720"
     CDS             complement(1781..1990)
                     /locus_tag="PLX22_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20803.1"
                     /translation="MHATFNDLVTKIRNLSLTEKLQIKSIVEKSIIEERRKEIYDSYL
                     KTKLELKNNKLKFTSDVKKLKKTID"
BASE COUNT          759 a          270 c          414 g          726 t
ORIGIN      
        1 gggtggagga taaggcaaca tcagacggct acagcacacc gtacaaattc accgggaagg
       61 aattggatgc ggaaacgggg ttgtactact ttggtgcacg gtattacgat gcgagggtaa
      121 gtagatggat atcggccgac ccggcgcttg cggacggtaa atattttccg aaacctaatg
      181 attatgacac agaacatgat ttttgctggt acttacagca ggatggcagt aagaagttag
      241 cgggattggg aggggtgttt aatgcagtaa atatggatgt gtatcactat gctggggata
      301 atccggtgaa actggtggac ccggatggga atcaatcatt gaatccactt gatttaggat
      361 taacagatcc aatatcattt gtaatagata tattacgata taaatatgaa acgccattta
      421 atgaagcaaa aaataagatt acatcaacat caataggttt tgcaacagga tttgtgaaag
      481 gattttttgg accagatttt tcaaagctta tagagaaatg tggtaaaaat atgaatggtt
      541 tttatgcaga agaatttcaa gcagctatag atagtggtga attaacaggg atggtagcag
      601 gattattaaa ttttacagca ggagcgtata agtcaggtca aacaatggag gctggctatg
      661 aattaggaac cggaatagta aataaaataa tatctgatcc aaaaacaaga aaagaatttc
      721 ttttaggcta tgcaaaagga tttgtgccat ctgctaaacc tccaaagttt aatagtattg
      781 tagaagcagc aggatggcta ataggaaata aatcatatca aaaatattgg gaagaacaaa
      841 ataaataaag tatttatata tgaatagaac agctataaca atatggttta tgatatctcc
      901 attttgtatt gatgtcggat ggaccggatt attaagagga tattcttatt taaaatttgt
      961 taagtacaca ggatttatag gtaatttatt gccaattttt gatattataa taggtttttt
     1021 tggattttat atgtactttt ggatggttat agtagaaagt aaaaggagta taaatataat
     1081 tcatataatg agagaattaa ttaaatcttc ttttttatat tcttgtttaa gtcttttaat
     1141 atttacatat attgtagaaa aaatatggga tttagagttt gtaacatata tatcattata
     1201 tatatcatta ttaattatat gcttatatac tcaagatata ttgagtgtaa aattatggca
     1261 tgtagtggaa caacaagaat ttaaatgtat tcataatcaa gaaaagaaaa aaaagtgatg
     1321 tagattgttt cagtaaaaat ttataattct ctttatatta ttagtaacta tcgccaaaat
     1381 aaaaaaagtc agtgggtgct tgcagtggta acggctgatg acggcactgg cgcacttgat
     1441 ggcgatggtg caagcgtgga tcgttataat tggataatgg tgtaaaagta gatatttaag
     1501 ttattttatt aatacacttc ttcatgagaa cctatatcga taaagatagc ttttgagttg
     1561 ttttcttcaa aaaagaagat aacacgcatg tcatattcta ccgaaaagct ccacaaattt
     1621 tttagctttc ctgaaagctt atgggtctta agcgaactgt gaaagggatc ctgtataaaa
     1681 agagaaactg cttcccagaa taaatcttca atttcgtttc tatttttgat ttttttctta
     1741 aatgctcttt taaacgatga actaaatgct atttccatgt ttagtcaatc gtctttttta
     1801 gcttcttgac atcgctggta aattttaatt tattattttt caattccagt tttgttttta
     1861 agtaactgtc ataaatttct tttcttcgtt cctcaataat tgacttttca acaatagatt
     1921 tgatttgtaa tttttcagtc aatgataaat tcctgatttt ggttacaaga tcattgaatg
     1981 ttgcatgcat aaaaaactcc gtatactggt ttataacaaa gtaagaatac attagcacga
     2041 ttaatataaa taatatccaa taaaaaatca ataacaatta tatagatata tagtaaaaca
     2101 tagtaaaaat tttaaagcat gcagtatcag tatgatgtat ttggcaggct taccatgctg
     2161 tggggtccg
//
LOCUS       DAOSOX010000304         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_50852_length_2162_cov_8.719506, whole genome shotgun sequence.
ACCESSION   DAOSOX010000304 DAOSOX010000000
VERSION     DAOSOX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50852_length_2162_cov_8.719506"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..653
                     /locus_tag="PLX22_12725"
     CDS             45..653
                     /locus_tag="PLX22_12725"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOT20804.1"
                     /translation="MPEPLRMSGEDVAKAQLQANEVNATLKAKKGKLPAELDKDSFLK
                     LLVAELRHQDPTQPMNDREFISQMAQFSSLEQMSNMNQAIERLVLRSQSGEAFSLIGK
                     EVEAVDFVSQQLVRGVASGVVYRQDGVKIIVGSTEVSLNDIHAVYMPKNNLTNENKKT
                     QSNEITEKTNNALNEQTKGMQQYQMNNTFVRGSSSTIQPLRN"
     gene            673..2043
                     /gene="flgE"
                     /locus_tag="PLX22_12730"
     CDS             673..2043
                     /gene="flgE"
                     /locus_tag="PLX22_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOT20805.1"
                     /translation="MMRSLFAGVSGLKNHQTRMDVIGNNISNVNTYGFKYSRVTFQDM
                     LSQTISGAAKPEENRGGINPRQVGLGMNIAAIDKIFTQGSLQTTGVNTDMAISGDGFF
                     IVAEGQKHLYTRAGTFALDKDGTLVNPANGLRVQGWMATTNAAGEKVINTSGTVEDII
                     VPIYGKVPAKETNYVKYKCNIDSKLPIVPNDANAIMRTSAGVTTNIDIYDKLGNPHRL
                     TLNFWKSGVNEWTASAAITDTELVTLDVPNGGNQPNQINGSSRVVLKFSPEGRIMSVA
                     DANSPDEINQGKLEVNLSYRVAGDPTQRNVRLDFGSAGLVEGITQFSSPSTTKAVEQD
                     GYTMGYMESFTVDNSGIITAVYSNGTKQTVGQVATAVFTNPQGLIAAGDNMFELSNNS
                     GLPNIGPAGEAGRGTIVAGTLEMSNVDLSEQFTDMIITQRGFQANSRTITTSDQMLQE
                     LINLKR"
BASE COUNT          703 a          376 c          517 g          566 t
ORIGIN      
        1 tatcatgatg gtatgctgga tataataatt taaggagaat gacaatgccg gaaccattac
       61 gaatgtcagg cgaagatgtt gcaaaggcgc aactgcaggc taatgaggtt aatgctacac
      121 tgaaggctaa aaagggtaaa ttgcctgccg agcttgataa ggattcattt ttgaagttgc
      181 ttgttgctga actacggcat caggacccaa cacagccaat gaatgaccga gaatttataa
      241 gtcagatggc acagttctct tcactggaac agatgagtaa tatgaaccag gcaattgagc
      301 gtttggtact gcggtcacag tccggagagg cgttttcgct catcgggaaa gaggtggagg
      361 cagttgattt tgtttcacag caactggttc gtggtgttgc aagtggtgtt gtttacaggc
      421 aggatggagt gaaaattatt gttggttcta ctgaagtttc ccttaatgat atacatgcag
      481 tatacatgcc caaaaacaat cttacaaatg aaaataaaaa gacacaaagc aatgaaataa
      541 ctgagaaaac aaataatgca ttgaatgaac aaaccaaagg catgcagcag tatcagatga
      601 acaatacatt tgtcagagga agttcatcaa ctatacaacc attacgaaat tagataacag
      661 gagttttgcg atatgatgcg atcacttttt gcaggtgttt ccggacttaa aaatcatcag
      721 acaaggatgg atgttatagg caacaacatt tccaatgtta atacctatgg attcaaatac
      781 agcagagtca cattccaaga tatgctttca caaacaattt ctggtgctgc aaaacctgag
      841 gaaaaccgcg gtggtataaa tccgcgtcag gttgggctgg gtatgaatat tgcagctatt
      901 gataaaattt ttactcaggg aagtttgcag acaaccggtg tcaatactga tatggcaata
      961 tcgggtgatg gtttttttat tgtggcagag ggtcaaaaac atctatatac aagggctggc
     1021 acatttgctc ttgataaaga tgggacgctg gttaatcctg caaacgggtt aagagtgcag
     1081 ggatggatgg ctacaaccaa tgctgccggt gaaaaagtta tcaatacgtc tggaacggta
     1141 gaagatatca ttgttccgat ttatggaaaa gtacctgcaa aagaaaccaa ctatgtaaaa
     1201 tacaaatgta acatagattc aaaattacca attgtaccca atgatgccaa tgctattatg
     1261 cgaacatctg caggagttac aaccaatatt gatatctatg acaaattggg gaatcctcac
     1321 cgattaacgc tcaatttctg gaaaagcgga gtaaatgagt ggactgcttc ggctgctatt
     1381 accgatacag aacttgttac gttagatgta cctaatggtg gcaaccagcc aaatcagatt
     1441 aatggctctt ccagggtggt acttaaattt tcacctgagg ggcgcatcat gtcagtggct
     1501 gatgctaata gccctgatga aataaatcag ggaaagcttg aggtaaattt aagctatagg
     1561 gtagcaggtg acccaacaca gcgcaatgta aggcttgact ttggtagtgc agggcttgtg
     1621 gagggcatta cacagttttc ttcgccatca accactaaag ctgttgagca ggatggttac
     1681 accatggggt atatggaatc gtttacggtt gataactcag gaattataac tgcagtatat
     1741 tcaaacggaa caaagcaaac ggttgggcag gtagctacgg ctgtgtttac caatccgcag
     1801 ggtttgattg cagcaggtga taatatgttt gagttgtcca ataactcagg gttgccaaat
     1861 attggacctg ccggcgaagc tggcaggggt acgatagtag ctgggacact ggaaatgtct
     1921 aatgtagact tatccgaaca gtttactgat atgattatta cccagcgagg atttcaggca
     1981 aattcaagaa caataacaac atctgatcag atgcttcaag aattgataaa tctgaagcgg
     2041 taaagaatag agtaaatggg ggttttgaaa aacccccata gcatagagat gcttcaagaa
     2101 ttggtacatc tgaagcggta aagaatagag taaatggggg ttttgaaaaa cccccatagc
     2161 at
//
LOCUS       DAOSOX010000305         2155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_51093_length_2155_cov_4.533810, whole genome shotgun sequence.
ACCESSION   DAOSOX010000305 DAOSOX010000000
VERSION     DAOSOX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51093_length_2155_cov_4.533810"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1213
                     /locus_tag="PLX22_12735"
     CDS             77..1213
                     /locus_tag="PLX22_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOT20806.1"
                     /translation="MRCYMQIICLLYIVILCHVNVYANYEQKGSVGGEPLQDVFVISR
                     KDWLIVPYVNYKPETQLAGGAIFLTYFQDIVMNIPPIKLRPTTLQVSVTQTQNGQFMF
                     QVLPEFYFYRDRFHIKNDFQYMDYYDKFYGIGQDTSDKHNEYYRSRVFAFKNVAEYAL
                     VPKLYMGLIYHIDVRETTKAESGGLIDANLLPGAKEGLVSGLGFTVNYDNRDDIIYTR
                     SGSYANLSVIQYSEYIKSDFNYTNITFDFRYFEPFLFSHILAFQFYTGYIDGEAPFYL
                     LSMLGGDKLMRGLYQGRYRDNDVMVAQVEYRVPLFWRFIACGFAGAGEVAPGMDDFKV
                     DEIVYAWGGGLRFKLRANQDLNVRMDVGFSKNFIGIYATIGEAI"
     gene            1597..>2155
                     /locus_tag="PLX22_12740"
     CDS             1597..>2155
                     /locus_tag="PLX22_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20807.1"
                     /translation="MKRPVEVSTGELIEILDRYLQTQGKVHYAIKIVGYPGVGKSAIV
                     QQIAHKHNYLFIDTRLAFKENVDLGGYPVPDNNQQRMVYFRPRFIPPEKVPEGYNGIL
                     WFLDEANRAHPTVIQTLFQIITERMCGEHALPDKTVIVLAGNLGDLDNTVITDFDDSA
                     LDGRLAVFNLIPDAQNWLQWALDEGI"
BASE COUNT          669 a          338 c          473 g          675 t
ORIGIN      
        1 ctccatacta gttattatgt caaaatacta tttaaagagt tttaattaac aggatagtgc
       61 tatcgggaga atgacagtgc gttgttatat gcaaataatt tgtttactgt atatcgtcat
      121 actctgtcat gtcaatgtgt atgctaacta tgagcaaaaa ggaagtgttg gtggtgagcc
      181 tttacaggat gtatttgtca taagtagaaa agattggctg attgtaccat atgtaaatta
      241 taaacccgaa acacagctgg ctggtggtgc tatattcctt acctattttc aggatatcgt
      301 tatgaatata cctcctataa aattaagacc aacaacattg caagtttcag ttacgcaaac
      361 acaaaatggt cagtttatgt ttcaggtatt accggaattt tatttttacc gggataggtt
      421 ccatatcaaa aatgatttcc agtatatgga ttattatgat aaattttatg ggataggtca
      481 ggatacctcg gataagcata atgaatatta ccgttcaagg gtatttgcat tcaagaatgt
      541 tgctgaatat gctcttgttc ctaaattata tatgggatta atctatcata ttgatgtcag
      601 ggaaaccaca aaagctgaaa gtggcggttt aattgatgca aacctgctgc ccggcgcaaa
      661 agagggattg gtgtcagggc tgggatttac tgtgaattat gataatcgtg atgatattat
      721 ctatacccga agtgggagct atgcaaattt aagtgtaata cagtattccg aatatataaa
      781 aagtgatttt aattatacca acattacctt tgattttcgt tattttgagc catttttgtt
      841 ttcacatata ctggcttttc agttttatac tggatatatt gatggagaag caccatttta
      901 cctgctttca atgttgggtg gcgataaact tatgcgcggt ttgtaccagg gcagatacag
      961 ggacaatgat gtaatggttg cacaggttga ataccgggtg ccacttttct ggagattcat
     1021 tgcctgtgga tttgcaggag caggagaggt tgccccgggg atggatgatt ttaaagtaga
     1081 tgagatagtg tatgcatggg gcggtggatt gcgatttaaa ctaagggcaa accaggattt
     1141 aaatgtacgg atggatgttg ggtttagtaa aaattttatt ggtatttatg caacgatagg
     1201 agaagcaatt taaaatagaa aggattctga cattatacta tcataattga aagttaaaaa
     1261 gcagattccg ggtcaagccc ggaatgacaa tgtgtggagt ctggaatgtt gaacgtcatc
     1321 atgaactggt ttcaggagct aacgctgcat attttgattc cggaacatgt cctgaatgac
     1381 gatatggtga catgtcattc tgaagcaaag ccgaagaatc cggtcgttca aaggagatgc
     1441 ttcggtcact aacgcttttt cagcatgacg cagtactatc ccatttttga aagctcacag
     1501 aataaaatgt tgaataatca tcatttcaac gatacaatga atatcatagg ttgctatcga
     1561 ttaaattatt atctttttca attattatga tgtgctatga aacgaccggt agaagtatca
     1621 acaggtgaat taatcgagat tctggatcga tatcttcaga ctcaggggaa ggtccactat
     1681 gccattaaaa tagttggata tcccggagtt ggaaaatcag ctatagtcca gcagattgca
     1741 cataagcata attatttatt tattgatact cgtttagcat ttaaagaaaa tgttgatctg
     1801 ggtggttacc ccgtaccgga taacaaccaa caacgtatgg tatatttcag acctcgtttt
     1861 ataccaccgg aaaaagtacc tgaaggctat aatggaattt tgtggttttt agatgaagcc
     1921 aacagggctc atccaacggt aatacaaaca ttattccaga ttatcaccga gcgtatgtgc
     1981 ggtgagcacg cattacccga taaaacagtt atagtattgg caggcaattt gggcgatttg
     2041 gacaataccg taattactga ttttgatgat tcagccctgg atggaagatt agcagtattt
     2101 aatcttatcc ctgatgcaca aaactggtta cagtgggcac tggatgaagg aatac
//
LOCUS       DAOSOX010000306         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_51176_length_2152_cov_8.535527, whole genome shotgun sequence.
ACCESSION   DAOSOX010000306 DAOSOX010000000
VERSION     DAOSOX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51176_length_2152_cov_8.535527"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1255
                     /locus_tag="PLX22_12745"
     CDS             62..1255
                     /locus_tag="PLX22_12745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOT20808.1"
                     /translation="MRQTIVIIAIIALTGTIPLFAQTVKKSTPVDKLNNKKTTDVKAD
                     EQSTVINKSKTISNTPKQNKKNTQETATTKQKSDIKQDKNPEAEKKKVEWIKNTMRYG
                     IHSDRIDAIKSALTLKEQSNKDDVMKLLNELMPQETNPEVKRTAITIAGELKSTSSVS
                     WIINSLNNQSEEVRIEAVYSIERLKATEAIPKLVSIVKEQNFTENSNFTEALINTLGK
                     LKATELKDFAIERIKETKTTDNLRQLLVLFLGEIEDISAKDFLLQLLKDKDENPTIRS
                     YAAVSLSKIGAKDTANDILEILSEIDSYPLNKRKDYYSLSMYCTASLVKMGDSNAIPR
                     LMEALRSDNPVVRLKAVELMKDLKDKRTVDILKYKMNYDPSPKVQKAAREALKAMGEE
                     VDESK"
     gene            1656..>2152
                     /locus_tag="PLX22_12750"
     CDS             1656..>2152
                     /locus_tag="PLX22_12750"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT20809.1"
                     /translation="MPLVSDFDKYFLHLPRISRPDDFDDFWHTAINELKKTPIDVQFI
                     HNKRKSTGKFTAYDMTYIGYNKVQLFASLHIPDKINKPHVVILFHDYDDVTSHAQFPI
                     DLPFAYCFVELRSHRNIIHYDEVEKKSPGFMVEGILEKNDYYVKGLYLDGLRTIDALR
                     LHNDL"
BASE COUNT          824 a          355 c          339 g          634 t
ORIGIN      
        1 atgatttaca atgggagttt acaatgtcac attatatttt aactatggaa tattatacaa
       61 tatgaggcaa actattgtca ttattgctat cattgcatta acagggacga ttcccctttt
      121 tgcacaaaca gtaaaaaaat ctactcctgt tgataagctt aacaacaaaa aaacgacaga
      181 tgtaaaggca gacgaacaat caacagttat caataaaagc aaaaccatca gtaatactcc
      241 aaagcaaaat aaaaaaaata cacaggaaac tgcaacaaca aaacaaaaat ctgatataaa
      301 gcaggacaaa aatcccgaag ctgaaaagaa aaaggttgaa tggataaaaa ataccatgcg
      361 ctatggtatt cacagtgaca ggattgatgc tatcaagtcc gcattaacgt taaaggaaca
      421 atcaaacaaa gatgatgtga tgaaactttt aaatgagctt atgccccagg aaaccaatcc
      481 tgaggtaaaa cgaaccgcaa ttaccattgc aggtgaactt aaaagcacat cgtctgtttc
      541 atggatcatc aattcattaa acaaccaatc cgaagaagtt cgcattgaag cggtatattc
      601 aattgaaaga ttaaaagcaa ctgaagctat tcccaaattg gtgagtattg tcaaagaaca
      661 aaattttact gaaaattcaa actttacaga agcccttatt aatacactgg gcaaattaaa
      721 ggcaactgaa ttaaaagatt ttgccattga acgtattaaa gaaacaaaaa ctactgataa
      781 tttgcgacaa ttattagtat tgtttcttgg tgaaatagag gacataagcg ctaaagattt
      841 tttattacag ttattaaaag ataaagatga aaatccgacg ataaggtcat atgcagcagt
      901 ctcgctttct aagataggtg caaaggatac agctaacgac attctggaaa tactatccga
      961 gatagatagc tatccattaa acaaaagaaa agattattat tcattatcca tgtactgtac
     1021 ggcatcatta gttaaaatgg gggacagcaa tgctatacca agactcatgg aagcacttcg
     1081 aagcgataat ccagtagtca ggcttaaggc agttgagctt atgaaggatt taaaagacaa
     1141 aagaactgtt gacattttga aatataaaat gaactatgat ccaagtccaa aggtacaaaa
     1201 agcagccaga gaagcattga aagcaatggg tgaagaagta gatgaatcaa aatagcttac
     1261 atgttaagac cgtactatcg caatcaatga cactatgtgt ggcaatactc attagcttaa
     1321 tactgataat attaatctca aacatactta ttttcccaat aatctatttt tcaacatcta
     1381 atccaaaatc atttaccctg actgtagaaa ttgtattgta tagcatatta atagctttgc
     1441 ttttatatag tattatttat agattaataa tacaaaaaaa agctggtatt tcttttgatg
     1501 tgactatcaa acatttttta tttactgctg tacaatacat agctctattt tttatagtta
     1561 ctactataat tattgttttg atagcaatta tatattattt acttaaaaca aattatatat
     1621 tgctttataa actaatacat taaaacaata ctacgatgcc cctagtaagc gattttgata
     1681 aatatttttt acaccttccc agaatatcac ggccggatga ttttgatgac ttctggcata
     1741 ctgctattaa tgaattaaaa aaaacaccca ttgatgttca atttatccac aataaaagaa
     1801 aatccaccgg gaagtttact gcctacgaca tgacctacat tggatataac aaggtgcaac
     1861 tttttgcatc attgcatatt cctgataaaa tcaacaagcc tcatgtcgtt atcctttttc
     1921 atgactatga tgatgtaaca tcacatgctc aattccccat tgatctgccg tttgcatact
     1981 gttttgtaga actacgaagc catagaaata ttattcacta cgatgaggtt gaaaaaaaat
     2041 cacctggatt tatggttgaa gggattttag aaaaaaatga ctattatgta aaaggtttat
     2101 atctggacgg cttaaggact attgatgcct tacgattaca taatgatctt ga
//
LOCUS       DAOSOX010000307         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_51409_length_2145_cov_10.800478, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000307 DAOSOX010000000
VERSION     DAOSOX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51409_length_2145_cov_10.800478"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLX22_12755"
     CDS             <1..1068
                     /locus_tag="PLX22_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOT20810.1"
                     /translation="TIKSHGQQETVQSNINSIEDSVWWAIVTSATVGYGDKYPISRLG
                     RFIGILTIFFGMALVGLITGNIASTLVERQLKEDRGLKQVKIKNHYVICGWKKDMAMV
                     LSNIAMRQGDFTGYDIVLVCMIDPENIENLKSNILFSNIHYVYGDHIDEHVLNRANIQ
                     YAKKVLVLADELGKSGQDADSRTVMSVMSIKAISRSVYVCAEIIDQKYERYLRLSGCD
                     EVILSNNYNKNIIADAFAKSGLAHVIGELVDASTPVNLTTEDISQHYIHKTYGELFDH
                     YKTKNKILIGLLENTGNFYDRKRFALQEAQKTPDISRLVDNLRIVKSMMSNYPVLNPK
                     KSYIIKNFSKAVLIEGSTENG"
     gene            1078..1608
                     /locus_tag="PLX22_12760"
     CDS             1078..1608
                     /locus_tag="PLX22_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOT20811.1"
                     /translation="MKLYKSYIDETILIKLQDLSLFKGLSQNVIRNIALVCTVKKIKR
                     GKCIIQEGDMGDELYIILNGEIEIQKRTLQNEPYTVVTMNANETPLYVGELAMIDNDK
                     RSATVIAKQDCDCLVLKRKDFIKFGDRNPKEGLILTRAIALKLAQSLRRTNDDVITLF
                     SALVDEISGETEPGKS"
     gene            1684..>2145
                     /locus_tag="PLX22_12765"
     CDS             1684..>2145
                     /locus_tag="PLX22_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20812.1"
                     /translation="MKKIMFIIIFLYIVSCISYAQEKRTMQQEADDKKFQQGLFFLSI
                     NEKQKALNEFYEYLDIYVDGLYRAKAYRFIAAIYFDNSNFTKAIAIYKRLFEEFPLDD
                     DGMYGLLQIGICYSKMGFDEEASKTFTQLMKEFPDSSYAKDAVTMLELLKIA"
BASE COUNT          777 a          267 c          427 g          674 t
ORIGIN      
        1 acaataaaaa gtcacggtca gcaagaaaca gttcaaagca atattaattc tattgaagat
       61 tcagtgtggt gggcaattgt aacatcagca acagttggct atggagataa atatcccatt
      121 tcacgattgg gcagatttat aggcatactg actatttttt ttggaatggc tctggtaggt
      181 ttgattactg gtaatatcgc atcaacctta gtagaaagac aattaaaaga agacagagga
      241 ttaaagcagg tgaagattaa aaatcattat gttatctgtg gttggaaaaa agatatggca
      301 atggtattat caaacattgc aatgcgacag ggagatttta ctggctatga tattgtgttg
      361 gtttgcatga ttgatcctga aaatattgaa aatttaaaaa gtaatatatt attttcaaat
      421 attcattatg tatatggaga tcatattgat gaacatgtgt taaatcgtgc taatatccag
      481 tatgcaaaga aggtattagt gcttgccgat gagttgggta aatcagggca ggatgcagat
      541 tccagaacag ttatgtcggt gatgtcaatt aaagcaattt caagaagtgt ttatgtatgt
      601 gccgagataa ttgatcagaa atatgaacgg tatttaaggc tgtcaggatg tgatgaggta
      661 atactatcaa acaactacaa taaaaatatt attgcggatg cttttgcaaa aagtgggtta
      721 gctcatgtaa ttggtgaatt ggttgatgcc agcaccccgg taaatttgac aacagaagac
      781 atttctcaac attacataca taaaacatat ggagagcttt ttgatcatta taaaacaaaa
      841 aacaaaatat taattggact tcttgagaat accgggaatt tttatgatcg taaacgtttt
      901 gcattgcaag aagcacaaaa gacacctgat atttcaaggt tagtggataa tttgcgaata
      961 gtcaaaagca tgatgtcaaa ttatccagta ctaaacccta aaaaatcgta tattattaag
     1021 aatttttcaa aagcagtact tattgaaggt tcaactgaaa atggttgagg taatgtgatg
     1081 aagttatata agtcatatat tgatgaaaca attttaataa aattgcagga tctttcgctt
     1141 tttaaagggt taagtcaaaa tgtaatacgc aatatagcat tggtatgtac tgtaaaaaaa
     1201 attaaacgtg gtaaatgtat aattcaagaa ggtgatatgg gtgatgagct atatattatt
     1261 ctgaatggtg aaatagaaat acagaagaga acattgcaga atgagcctta tactgtagta
     1321 acaatgaatg ctaatgaaac ccctctttat gtaggcgaac ttgcaatgat agataacgat
     1381 aaacgttcag caacagttat tgcgaaacag gattgcgatt gtctggtttt gaaacgaaag
     1441 gattttatta aatttggcga tagaaatcct aaagaaggct tgatactaac aagagctatc
     1501 gccctgaaat tagctcaaag tcttagacga acaaatgatg atgtaattac actttttagt
     1561 gctttagttg atgagatttc aggagagact gagcctggta aatcataaaa tggaaacgat
     1621 attttttttg aatctgagtt tgtttttgaa gattgggagt aattcatttt tggagaagaa
     1681 acaatgaaaa agataatgtt tattataata tttttatata ttgtaagttg catctcttat
     1741 gcacaggaaa aacgtacaat gcaacaggaa gccgatgata aaaagtttca acagggattg
     1801 tttttcttat cgattaatga aaaacaaaaa gcattaaatg agttttatga atatcttgat
     1861 atttatgttg atggactata tagagcaaaa gcttatcgct ttattgcagc aatatacttt
     1921 gataattcca attttacaaa agctatcgcc atttataaac gcctttttga ggaattccct
     1981 ttggatgatg atgggatgta tggcttgtta cagattggaa tatgttacag caaaatggga
     2041 tttgatgaag aagcatctaa aacatttact cagcttatga aagaatttcc cgattcaagc
     2101 tatgcaaaag atgccgtaac tatgcttgaa cttttaaaaa tagca
//
LOCUS       DAOSOX010000308         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_51556_length_2141_cov_6.413231, whole genome shotgun sequence.
ACCESSION   DAOSOX010000308 DAOSOX010000000
VERSION     DAOSOX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51556_length_2141_cov_6.413231"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLX22_12770"
     CDS             complement(<1..368)
                     /locus_tag="PLX22_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT20813.1"
                     /translation="MEAVTTTKVPGARKLFSGKVRDVYKADDEHLIIVSTDRVSAFDW
                     VFPNGIPGKGVILNTISNLWFKHIRLINNHIVETNYKNFPKPFCDYPEFFKDRAVLVK
                     KAKRIDFECVARGYIIGSAW"
     gene            complement(422..1855)
                     /gene="gatB"
                     /locus_tag="PLX22_12775"
     CDS             complement(422..1855)
                     /gene="gatB"
                     /locus_tag="PLX22_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT20814.1"
                     /translation="MEFDTVIGLEVHAQLNTQSKIFCSCSTQFGAQPNTQVCPVCLGL
                     PGVLPVLNKEVLKKAIMAGLALNCTVAHYSKFDRKNYFYPDLPKAYQISQYDKPICYD
                     GYIEISIKDETKKIGITRLHMEEDAGKTIHSDDANNKVSYVDFNRTGVPLIEIVSEPD
                     ITTPDEAYQYLQTLRSILKYIEVSDCNMEEGSLRCDVNVSLKPKGAKEFGQKVEIKNL
                     NSFRSVKLALEYEIGRQQKMLMQDETIVQETRLWDAERNITQAMRSKEEAHDYRYFPE
                     PDLPPIEISNDYVEQLKNELPELPYEKCDRFVKQYGLPRYDAQVLTVTRQLALYYETV
                     VSNGAHPKKASNWIMSELLAKIDDPEKIDTFIVSPQNLAILLKRIDDATISGKIAKTV
                     FEEMLKTGNDPDAIINEKGLRQVTDTAAIESIIDEVIKSNPKSVEDFRSGKDKALKFL
                     IGQVMKESRGKANPQIVNDIMIQKLSK"
BASE COUNT          649 a          392 c          326 g          774 t
ORIGIN      
        1 ttccatgcac tgccaatgat ataaccgcgg gcaacacatt caaaatcaat acgctttgct
       61 ttttttacca gtactgccct atctttaaaa aactcagggt agtcacagaa tggtttggga
      121 aaatttttat aatttgtttc aactatatga ttatttatta accgtatatg tttaaaccat
      181 aaattggaga tagtatttaa aattacacct ttgcccggta tcccattggg gaatacccaa
      241 tcaaatgcag ataccctgtc ggtagaaaca ataataagat gctcgtcatc tgctttatac
      301 acatcacgca ccttgccact gaataatttt cgtgctcccg ggacttttgt tgtggttacc
      361 gcttccatat attatccttt caaaaaaata tttttatgtt cgatagttac tgtttaactt
      421 tctattttga aagcttctga atcataatgt cgttaacaat ttgcgggttg gctttccctc
      481 tggattcttt cataacctgg ccaattaaaa atttaagtgc tttatccttg ccacttctga
      541 aatcctcaac agatttagga ttgcttttaa taacttcatc aataattgat tcaatagctg
      601 cagtatcggt aacctgacgc aagccttttt cattaataat agcatcgggg tcattacctg
      661 tttttagcat ctcttcaaag actgttttag caattttacc acttatggtt gcatcatcaa
      721 tcctttttaa taggatggct aaattttgcg gcgaaacaat aaaagtatct attttttcag
      781 gatcatcaat ttttgctaaa agttcgctca ttatccagtt agatgctttt ttaggatgtg
      841 ctccgttaga tacaacagtt tcgtaataca gagctaactg tctggtaacc gttaatacct
      901 gcgcatcata tctgggcaat ccatattgtt taacaaagcg atcgcatttt tcgtatggaa
      961 gctcgggcaa ttcatttttt aactgttcta cataatcatt tgatatttca attggcggta
     1021 aatcaggttc aggaaaataa cgataatcat gtgcttcttc ttttgaacgc atagcctggg
     1081 taatatttcg ttcagcatcc catagacgtg tttcctgaac aattgtttca tcctgcataa
     1141 gcattttttg ttgtcgtcca atttcgtatt ctaatgctaa ttttaccgat ctgaagctgt
     1201 tcagattttt aatttcaact ttctgtccaa actcttttgc tcctttaggt tttaaagaaa
     1261 cattgacatc acatcgcaag ctaccttctt ccatattgca gtcagatact tcaatatact
     1321 ttaatattga acgtaatgtt tgtaaatact gatatgcttc gtcaggagta gtgatatcag
     1381 gttcagaaac aatttctatt aaaggaacac cagttcggtt aaaatcgaca tatgacactt
     1441 tattattagc atcatcactg tgaatagttt taccggcatc ttcttccata tgaaggcgtg
     1501 taatgccaat tttttttgtt tcatccttta ttgatatttc aatataaccg tcatagcata
     1561 taggtttatc atactgcgaa atctgatatg ccttgggtag atcaggataa aaataatttt
     1621 tacggtcaaa tttgctatag tgagcaactg tacagtttag tgcaagtcca gccattattg
     1681 ctttttttaa aacctctttg ttaagaacag gcaacacccc tggaagtccc agacataccg
     1741 gacaaacctg agtattgggc tgtgccccaa actgcgttga acaactacag aatatttttg
     1801 attgtgtatt tagttgagca tgaacttcca gtccaataac cgtatcaaat tccatacata
     1861 ttgtaccatt tttattctat aaaggtgata atagctttac tgatattctt ttcggatagt
     1921 agatagtatt atttttgtca actaaaattg attttatttc tatcatatat tattgttggc
     1981 aataatttct acaataaaaa tcataatact attgaaatac taaatttttg tattaaaatg
     2041 tataaaaatc ctgcaacaaa gtagttagta ccttttctat ttaatgtaaa agtaatgtat
     2101 cattattgac aaaataacat aaaaaaatac tatcgcaaga a
//
LOCUS       DAOSOX010000309         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_51739_length_2135_cov_10.014904, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000309 DAOSOX010000000
VERSION     DAOSOX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51739_length_2135_cov_10.014904"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLX22_12780"
     CDS             complement(<1..863)
                     /locus_tag="PLX22_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HOT20815.1"
                     /translation="MDGFKVFSFIGIIICMGMTMVFAQTQTEADKTNDENNGMVILEE
                     IVVEGAKLIIPTKQAGETVYTGTEVTRKGMELKGEKGKANVYESISIVPGVVFEGIDP
                     GNMVSEQNSIRMRGVRGFMGAMTVDGIPNYGGNPMGPRAYVYDMENFDSIAVFKGAVP
                     ADLGVGVGDRGGAVELKPRWAQDTFGIALVSSYGSFDLMKMFVRVDSGKVGPADSRLS
                     VSYSCTKEDKWKGPGDLGPRHNANVTFVQPMGKYMTISLWGNYNTIHHYKYRSLSYEQ
                     AKDLDDYYKYD"
     gene            1094..>2135
                     /locus_tag="PLX22_12785"
     CDS             1094..>2135
                     /locus_tag="PLX22_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20816.1"
                     /translation="MNDDWYNTAILQSIIIADAFASPLDNLSAQHIKATFDDVTEYVD
                     PEPALKHKLYLWKKPGLYTSLSQYCILLCAINSQNYTYNHQLVINFLHSLGDSSAYFR
                     HTPQILHNLGNAYDAPTAELLVLIPSLLFCKREPTPQNLLHFILAHNKNAATCAACTF
                     FYLLLKEIIGRQLSTFHPEILNDISSQMALLIQQHSASIFEHGANPQVITQAASDLHA
                     LMTTLPLTSDEVTFTRHILNYANKWSNNSFTRLTVNHPFAILPFALYCSYSTPYNAVF
                     SHIHHGGRVSVLLPLIAAISAALYGPDSIPEHLKETLINKKRIYQLLQFLHESSITMN
                     YLTEFFQNEMKLT"
BASE COUNT          658 a          519 c          314 g          644 t
ORIGIN      
        1 aaatcatatt tataataatc atctaaatcc ttagcctgct cataggataa tgagcgatat
       61 ttataatgat gtattgtatt ataattaccc cacaatgaaa tagtcatgta ctttcccata
      121 ggttgaacaa aagtaacgtt tgcattatgc cgtggcccta aatccccagg gcctttccat
      181 ttatcctctt tagtgcatga atatgaaacc gataatctac tgtcagccgg accaactttt
      241 cccgaatcaa ctcgtacaaa catcttcatg agatcaaaac taccataaga agaaacaagc
      301 gcaatcccaa acgtatcctg cgcccatcgt ggctttagct caaccgcacc gcctctatca
      361 ccaacaccca cacctaaatc agcagggaca gcgcccttaa acacagcaat gctatcaaag
      421 ttttccatat catacacata agccctgggc cccatcggat taccaccata gttaggtata
      481 ccatctacgg tcatcgcacc cataaatcct ctaacgccac gcattctgat gctgttttgc
      541 tcagaaacca tattaccggg atcaattcct tcaaagacca cccctggtac tatacttatt
      601 gattcatata catttgcttt acccttctca cctttaagct ccatcccttt ccgtgtaacc
      661 tcggtcccgg tatagaccgt ttcccctgcc tgttttgtag ggataatcaa cttcgcaccc
      721 tcaactacta tttcttctaa tataaccatt ccattgtttt catcatttgt tttatccgct
      781 tccgtttgcg tctgggcaaa taccatcgtc atacccatgc atataatgat gccaataaaa
      841 ctaaacactt taaaaccatc catctatacc ttccaaaaaa acaataatta tgataatatt
      901 gctacaagta tgctaataat agcacattaa aaaattatgt gttatttgtc aataatatta
      961 atggtatatt agtagcaata aatgctatta atacaatatt aataatatta tctgtatatt
     1021 atagatataa tattactata gcgtatagta aattaagctt gactgctttc tcgttatttt
     1081 tatcatacca ttcatgaatg atgattggta taatactgcc atattacaaa gcatcattat
     1141 tgccgatgca tttgcaagcc cccttgataa cctttccgcc cagcacatta aggctacctt
     1201 tgatgatgtt accgaatatg ttgacccgga accggcgttg aagcataaac tttatctgtg
     1261 gaaaaagccg ggactttata catcgttatc gcaatactgt attttgcttt gcgctataaa
     1321 ctcgcaaaac tatacctata atcatcaact ggtcattaat tttttacatt cactgggcga
     1381 tagttctgca tatttcaggc atactcccca gatattgcac aaccttggca atgcctatga
     1441 tgcacccact gcagaattgc tggtattgat accttcattg ctattttgca aacgcgaacc
     1501 aacgccacaa aatttgttgc actttatcct tgctcacaat aaaaatgcgg caacctgcgc
     1561 agcctgcacc ttcttttatc tgttattgaa agaaattata gggcgacagc tcagtacatt
     1621 ccatcctgag attttaaacg atatatcgtc acaaatggca ctgcttatac aacaacactc
     1681 tgcatcaatc tttgaacatg gagcaaaccc gcaggtgata acgcaagctg caagcgacct
     1741 gcatgcactg atgacaacgc taccgcttac aagcgatgaa gtaaccttca cacggcatat
     1801 tcttaactat gccaataagt ggagcaacaa cagttttacc cgcttaaccg tcaatcatcc
     1861 ttttgcaatt ttgccatttg ctctttactg ctcatattcc acaccatata atgcagtatt
     1921 ttcccatata caccacggtg gcagggtctc ggttcttttg ccacttattg cagcaatttc
     1981 agcggcatta tatggccctg acagtatccc tgaacattta aaggaaacac ttataaacaa
     2041 aaaaaggata taccagttgc tgcagttttt gcatgaaagc tcaatcacca tgaactatct
     2101 aacagaattt tttcaaaatg aaatgaagct aacgc
//
LOCUS       DAOSOX010000310         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_53329_length_2087_cov_5.694882, whole genome shotgun sequence.
ACCESSION   DAOSOX010000310 DAOSOX010000000
VERSION     DAOSOX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53329_length_2087_cov_5.694882"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..332)
                     /locus_tag="PLX22_12790"
     CDS             complement(123..332)
                     /locus_tag="PLX22_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20817.1"
                     /translation="MVLHALENNLPGIKFDQHSDGIHVIITVPSIPPGSKKFTQINTT
                     IKKNYPLFNVIKFKYLQAMAKTTSH"
     gene            1006..1656
                     /locus_tag="PLX22_12795"
     CDS             1006..1656
                     /locus_tag="PLX22_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT20818.1"
                     /translation="MAILPELVKVFIVEDEDLVRDTLSQSLQLIDNFQIVGASANISS
                     SLDTIQSAMPDVCIVDVRLQNENGIEASKTLKKLLPDLKIIVLSGYFNETLAATALAS
                     GASAFLSKSIAVSHLVAAIFHVMVHNNFYAPQLPDDVLEGIIINSKTSKRLCALSKRE
                     KEILELIAREYSTREIAEKLGISEKTVRNHKSNIMAKLGITNHVGLIKYAYYMAMI"
     gene            complement(1657..>2087)
                     /locus_tag="PLX22_12800"
     CDS             complement(1657..>2087)
                     /locus_tag="PLX22_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20819.1"
                     /translation="IALGDEIQFYGRFLKLMGMTEFDTGHMAVFGGLNMNLHTSGIAC
                     MPHGVGTISFEAGKTFVKAQFENSMLLKDNHVYSIMLQHSTTGKPLPLYYTKQTTVTA
                     IDNYVTEVAIQFDKGEVKGKVNAYCIVDTYPVAMAELVIQ"
BASE COUNT          648 a          350 c          378 g          711 t
ORIGIN      
        1 cagaaaaggc atagcaacat tatgtatagg tggcgggcag ggttgtgcag taacagtgga
       61 gcgataaaga atatcactat atagaggcaa aaaagagggg agcgcattgc cccctctttc
      121 ttttaatgtg atgttgtttt agccattgct tgcaaatatt taaattttat tacattaaat
      181 aatggatagt tcttcttaat ggtagtattt atttgtgtaa acttttttga acccggtgga
      241 atgcttggta ccgttataat aacatgtata ccatcactgt gttgatcaaa ttttatacct
      301 ggcaggttgt tttccaacgc gtgcaatacc atattttgaa catccttttt tggttgctca
      361 agtttaaatg tttgataggt gtaaatccaa acggggattg caatggatgt taatattata
      421 atggaagcga cgattttgac ctaatgctcg ttgagtaacc ttttgtttgt cttccaatgt
      481 agggtgtatc ttcataatca agaatataaa gacaccagca agagagattg atattaaatt
      541 aaggtgctgc aatataaaat tgcagcacct tttgcaatag cccagtccaa attacctttt
      601 atattttatt ttttgcggat acctttcaga ttgctgattt tttgatagtt gtgtttcgtc
      661 attttatttc tttaatggat tattatcata ggtttttttt aaaaaatatc ttttatgttc
      721 attgttcaac ctctatactg cacgccataa cagtgccgtt gtactctatt gtagtagtga
      781 gtatagcaat actatttttt ttcctgctac ttttcaaaga taattgctgt aataataaaa
      841 aaataatgta tttaagtggc tataatgtga ttattgtgca aatggtataa tatctatttc
      901 aacaatggta tatatacaac aataaattat atattgggat atttgcctat tgactatttt
      961 tgtatatatt gtacaattaa agtcacaaaa aaataggaat agtatatggc aattctaccg
     1021 gaacttgtga aggtttttat tgttgaggat gaggaccttg tgagagatac cctgtctcaa
     1081 agcttgcaat taattgataa tttccaaatt gttggtgctt cagcaaatat ttcttcttca
     1141 cttgatacta tacaatctgc aatgccagat gtatgtattg ttgatgtgcg tttgcaaaat
     1201 gaaaatggca ttgaagcaag taaaacacta aaaaaacttt taccagattt aaaaataatt
     1261 gttttatcgg gatactttaa tgagacatta gcggcaactg cattagcatc gggagcatca
     1321 gcatttttat ccaaatctat agcggtatca catctggttg ctgctatttt ccacgttatg
     1381 gttcacaaca atttttatgc accacaatta cctgatgatg tgctggaagg tataatcata
     1441 aatagtaaaa cctcaaaacg cctttgcgca ttgtcaaaac gggaaaagga aatactggag
     1501 cttattgcca gggaatattc aaccagggaa atagccgaga agcttggaat cagtgaaaaa
     1561 actgttcgca atcacaaatc caatatcatg gcaaagcttg gcattaccaa ccatgtcggg
     1621 ttgattaaat atgcctatta tatggcaatg atatgatcat tgaatgacaa gctcagccat
     1681 tgcaactgga taggtatcaa caatacagta agcattaacc ttccctttca cttcaccctt
     1741 atcaaattgt atagctacct ctgtcacata attatcaatt gcggttactg tcgtttgctt
     1801 tgtatagtat aatggtaatg gcttccctgt tgtactgtgc tgcaacatga tgctgtaaac
     1861 atgattatcc ttcaatagca tcgaattttc aaattgagct ttcacaaaag ttttgcctgc
     1921 ttcaaaggat attgttccaa caccgtgcgg catacaggct ataccgctag tatgcaggtt
     1981 catgttcaat ccgccaaata ctgccatgtg accggtgtca aattcggtca tccccatgag
     2041 ttttaaaaat ctgccataaa attgaatctc atcacccaat gcaatgg
//
LOCUS       DAOSOX010000311         2083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_53466_length_2083_cov_7.236193, whole genome shotgun sequence.
ACCESSION   DAOSOX010000311 DAOSOX010000000
VERSION     DAOSOX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53466_length_2083_cov_7.236193"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLX22_12805"
     CDS             complement(<1..673)
                     /locus_tag="PLX22_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20820.1"
                     /translation="MKLFHLTVTITLIIIAFAMGTYIYPQGAKYYFDAGCRYYVHKNW
                     EKAKENFLKDIEVADRGDSYYFVGEIEKILGNLDSALHYFENAVTRKMTPKYRNLAYW
                     NLIVIYESRSEYAKTIKTCKYFYEVHGESSAKTKAENLINKLMWSENEDAKKEYEAGL
                     ELKMKDYTTALQHFRQAVYLDNSFLSPVFEIGMYYYSQENYRDALSYFNQILYKIPFY
                     GELQML"
     gene            complement(849..1637)
                     /locus_tag="PLX22_12810"
     CDS             complement(849..1637)
                     /locus_tag="PLX22_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOT20821.1"
                     /translation="MKKYYFISAITFTAIMLMCFIHAHSQNMPSAFSTVPIKNFTPKI
                     WDENFSLYMITSEQEILLITTPYQTILSTKNNNNRHAVLIKAGKNKDYAELPLNKDMK
                     DTRLLQLTHPLVQSYAKKIKASSNIVNEVEQLIYTTIETKTEGIPIIPAPIILETKKG
                     DCTEHAVTSIALLRALKIPARAVVGMIAAREYAGRKNVFVFHMWAEAYYNNTWILVDA
                     TRPGAKKPNLYIALTYHSLETEMPLEFLEAMAAIQNLTVTYLSE"
     gene            complement(1634..>2083)
                     /locus_tag="PLX22_12815"
     CDS             complement(1634..>2083)
                     /locus_tag="PLX22_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20822.1"
                     /translation="DCKPVKLEKHTYINAPAQQRLDIIAECNGKQITLTENNNKTVIV
                     LDKDYILDGNYFLYQLIKNKFKEGRTIRHNVYDPSIEREDAIPVTITLVGKETIAINS
                     IPYRAWRVDQSIGKIKNIIVYLDDKGVVLKTSISMLNMKLDVIRKGL"
BASE COUNT          616 a          362 c          370 g          735 t
ORIGIN      
        1 ccagcatctg gagttcacca taaaatggaa ttttgtacag tatttgatta aaataggata
       61 aagcatcgcg atagttttcc tgtgaataat aatacatccc tatttcaaaa actggtgaaa
      121 gaaatgaatt atcaaggtag acagcctgcc tgaagtgttg taaagctgta gtgtaatctt
      181 tcatttttaa ttccaatcca gcttcatatt ctttctttgc gtcttcattt tcggaccaca
      241 tcagtttgtt gataagattt tcagcttttg tctttgccga agactcgcca tgcacttcat
      301 aaaaatattt gcaggtcttg atagtttttg catattcaga ccttgattca tagataacga
      361 ttaagttcca gtatgccaga ttgcgatatt tgggagtcat tttgcgggtg actgcattct
      421 caaaatagtg caatgcagaa tctaaattac ccagaatttt ttcaatttcg ccaacaaagt
      481 agtacgaatc acctctatcg gcaacttcaa tatctttaag aaaattttct tttgcttttt
      541 cccagttttt gtggacgtaa taacggcaac cggcatcaaa ataatatttg gcaccctggg
      601 ggtatatata ggttcccata gcaaaggcaa tgatgataag ggtgatagtt actgttaaat
      661 gaaacaattt catagcacaa tccctgatgg tagtaacgtt ttgaaaaatc ctttcttgaa
      721 ttacagtgat gtattacaaa tatgtcaata cattaattgg attgctaccc tatatttaaa
      781 atcaatcata gcaatgttga ttaccgtgat acaagcactg taatatattg aaatgaatac
      841 tatgaacctt attctgaaag atatgttact gtaagattct gaatagctgc cattgcttca
      901 agaaattcca gcggcatttc agtttcaagg ctatggtagg ttagtgcaat ataaagatta
      961 ggctttttag ccccagggcg agttgcatca accagaatcc atgtattgtt ataataagct
     1021 tctgcccaca tgtgaaatac aaatacattt tttctaccgg catattccct ggcagcaatc
     1081 atccccacta ctgcacgtgc aggaatcttt aaagccctga gtaaagcgat ggaagtaacg
     1141 gcatgttctg tacaatcgcc tttttttgtt tccagtatta tcggtgcggg aattattggt
     1201 ataccttctg tttttgtttc gatagtagta tagatgagtt gttcaacctc gttaacaata
     1261 ttactggaag ctttaatttt ttttgcatag ctctgtacca atggatgagt taactgcaaa
     1321 agtcgagtat ctttcatgtc tttattcaat ggcaattcag catagtcttt atttttgcct
     1381 gctttgatta atacagcatg gcgattgtta ttgttttttg tagacagtat tgtctgatag
     1441 ggcgttgtaa taagcaaaat ttcttgctca gaggttatca tataaagaga aaaattctca
     1501 tcccagattt taggggtaaa attttttatt ggaacagtgg aaaaagctga tggcatgttc
     1561 tgcgaatgtg catggataaa gcacatcagc atgatagcag taaaggttat tgcactgata
     1621 aaataatatt ttttcataat cccttgcgga taacatcaag tttcatatta agcatactga
     1681 ttgaggtttt cagtaccact cctttatcat ctaaatagac tataatattt tttatttttc
     1741 caatgctttg atcaacccgc catgctctat acgggatgct attgatagca attgtttctt
     1801 tcccaacaag ggtgatagtt accggtattg catcttcacg ttcaattgaa gggtcgtaca
     1861 cattatgccg tattgttctt ccttccttaa atttattttt aataagctga tacagaaaat
     1921 aatttccatc aaggatataa tctttatcca atacgatgac ggttttattg ttattctctg
     1981 ttaaggtaat ctgcttccca ttacattcgg caataatatc taatctttgt tgtgcgggtg
     2041 cattaatata ggtatgcttt tctaatttta caggtttgca atc
//
LOCUS       DAOSOX010000312         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_54295_length_2060_cov_5.339651, whole genome shotgun sequence.
ACCESSION   DAOSOX010000312 DAOSOX010000000
VERSION     DAOSOX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54295_length_2060_cov_5.339651"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLX22_12820"
     CDS             complement(<1..484)
                     /locus_tag="PLX22_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT20823.1"
                     /translation="MNIPFHVPYIDDDDIDAAVGAIKSGWTTMGPKTIAFEDAFARYV
                     NVPNAVAVSSCTAALHLALKVCGVGSGDEVIVPANTFVATAEVVEYCGATVVFADIDK
                     QTHCIDSVSIQKKLSPKTKAVIAVHYAGHPCDMDTIMDSAKAHNCYVIEDAAHSLPSY
                     Y"
     gene            complement(1020..>2060)
                     /locus_tag="PLX22_12825"
     CDS             complement(1020..>2060)
                     /locus_tag="PLX22_12825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT20824.1"
                     /translation="KVSQKNGLYDYVNERKIHRGKISRLGGLATFIGFMCSLLYLLIY
                     DFKVQFNIPLFIAAVLLAFLTGFIDDIKKISARYKLLLQLACGLLVAIAGLQINRITI
                     SPGFSIQFGVLSYVVTMLWVAGFMNAINMLDGMDGLASGIVMIASLFLAIIGWLQGMV
                     VVTYLAIALMGAIGGFFIFNYPPAKIFMGDGGAYFLGFMYAILPLIGIKKVSTITVFF
                     VPLVLLLIPLIDMAHVVQNRFVGGNHIFLADRSHIHHRLLHIGFSNKGILWILYSYTA
                     ILGCFSIVLLYLKPHQSIIVMILLFLLTILAFFTISVAERRIAQLEGITNNKKSNKGL
                     LKHGAKHKFSKA"
BASE COUNT          689 a          421 c          333 g          617 t
ORIGIN      
        1 tataatacga tggcaatgaa tgggcagcat cctcaatgac atagcaattg tgcgcctttg
       61 cactatccat gattgtatcc atatcgcatg ggtgtcctgc ataatgaacg gctataacgg
      121 ctttggtttt tggcgatagt tttttttgaa tggatacact atcaatgcaa tgggtttgtt
      181 tatcgatatc agcaaacacc accgtggctc cgcagtactc aaccacctca gccgttgcaa
      241 caaaggtatt tgcagggact ataacctcgt cgccactgcc aacaccgcaa acctttaatg
      301 ctaaatgaag cgctgccgtg cacgagctta cagcaacggc attgggcaca ttgacatagc
      361 gagcaaaggc atcttcaaat gcaatggttt ttggacccat ggtagtccat ccgcttttaa
      421 tggcacccac ggcagcatca atatcatcat catcaatata ggggacatga aacgggatgt
      481 tcatggaagt atatccttat gcacaacatc gtattaattc tattttcgga taaaaacggc
      541 ggcaagaata tcaaaagatt ttaattataa aggaccctgc tcactgcttt aatttgtaat
      601 ttccaccttt ccaaaatatt ttctatactg caaaatgtca tgtgtgctat agtgattttt
      661 gttatgcgta acaaaatcta aatccttgct tttcctatga tgtaaatacg ctgctaacgc
      721 agagccatca actaaaacat agatgttgtt ttgtttgttt cataaacact tgcactatta
      781 ttacattatg gtcacgtata acattacgtt tttcattatt aaaaaaaata gtaaaccatt
      841 ttcttaatgc ttatatcttc aggatatacc agctataatg atgatagcct cccttattgc
      901 aaagctgcag tagcatagtg taaaacatta ttgttatcaa atgtttgttt ataggttatg
      961 ttatattatg ttacacacaa gcccacatta agggtatagt attcatgata cactgttaac
     1021 tatgcctttg aaaatttatg ttttgcacca tgtttcaata aacctttatt gcttttttta
     1081 ttattagtaa tgccttccaa ttgagcaata cgtctttcag caactgatat ggtgaagaaa
     1141 gcaaggatag tcaataaaaa taaaagtatc atgacaataa tagattggtg aggtttaaga
     1201 tacagcaaca caattgaaaa acatccaagg attgccgtgt agctgtataa tatccacaat
     1261 atccctttgt tactaaaacc aatatgaagc aatcggtgat gtatatggct tctgtcagct
     1321 aaaaagatat gattcccgcc tacaaatctg ttttgcacta catgtgccat atcaatcagc
     1381 ggtatcaata gcaacaccag cggtacaaaa aatactgtta tggtagatac ctttttaata
     1441 cctatcagtg gtaatattgc atacataaag cctaaaaagt atgcccctcc atcacccata
     1501 aaaatcttcg caggaggata gttaaaaata aaaaatccac cgatggcacc catcaatgca
     1561 attgctaaat aagtaaccac taccattccc tgtagccagc caataattgc caaaaacaat
     1621 gaagctatca ttacaatgcc actggcaagc ccatccatac cgtcaagcat attgatggca
     1681 ttcataaatc cagcaaccca taacatggta acaacatatg atagtacgcc aaattgtatg
     1741 ctaaatccag gagatatagt aatcctgtta atctgcaatc cagctatggc aacaagcaac
     1801 ccacatgcta actgtaatag taacttatag cgtgccgaaa tctttttaat atcatcaata
     1861 aatcctgtca aaaatgccag caacactgca gcaataaata gtggtatatt aaactgtact
     1921 ttaaaatcat atattaaaag gtatagcaac gaacacataa atccaataaa ggtagccagt
     1981 ccacccaatc tgctgatttt gcccctatga attttgcgtt cattaacata atcataaagg
     2041 ccattttttt gactaacttt
//
LOCUS       DAOSOX010000313         2057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_54414_length_2057_cov_6.081918, whole genome shotgun sequence.
ACCESSION   DAOSOX010000313 DAOSOX010000000
VERSION     DAOSOX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54414_length_2057_cov_6.081918"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>2057)
                     /locus_tag="PLX22_12830"
     CDS             complement(147..>2057)
                     /locus_tag="PLX22_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT20825.1"
                     /translation="IAVGMATNIPPHNLGEIIDAIVFMIDTPDATVKQLMKFVKGPDF
                     PTGATIVGSEEMVKAYAKGKGSVIIRAKLHIEEQKNKSLIVVTELPYQVNKAALIAKI
                     AELVNNKVIEGINELRDESDRKGLRIVIGCRKDAVTNIIINQLYKHTQLQTSFGINLL
                     ALVNNQPKLLDLKGLIFHYINHRKIVVTRRTQYDLAKARERAHILEGLKIALDNIDEV
                     IAIIRRSKTVEEANDHLIKRFKLSEIQSKAILEMRLQRLTSLEVKKVVDELKELHILI
                     EELSAILKSEKKIYAIVKEELLTIKAKYNDSRRTEIIFEDTSVSFDAEDLIAEEDMVI
                     TITNDGFIRRLSADTFKKQGRGGKGVIGMSSRKEDFISMMTIASTHDMLLLFSNKGKI
                     FGMKVYEIPIASKNNRGKSLKGIINLASGEIITAMCSVDDFSQNFLCMVTRNGIMKKT
                     ALEEFSNVKKGGIIAINLHKDDELVDVKVVKGSDDVVIATRKGLLLRTNIAKMRAMGR
                     NSAGIIGMRVGTDDQIIAMDVVKQNSDLCVVTSRGYGKKTQYALFATKGRGGKGMAYM
                     KIHDKNGYAVGVRSVYPEDEVIIASKKGMTIRLKAKDISYQGRAAAGVILVQLDGDDE
                     VTDFAVLYEVKE"
BASE COUNT          591 a          460 c          290 g          716 t
ORIGIN      
        1 ttctaaaaga agataaagca ataggtatgt tacaggaaat tcaatcaatc gataagtaca
       61 aagaaagcta tattgtaaaa gaataatttt gttatcctgc tactccctgc agcaggataa
      121 ctattattgt tactaatttc tttgtattat tctttaactt catataaaac agcaaaatcc
      181 gttacttcat catcaccatc taactgcacc aatataacac cagcagcagc tcttccctgg
      241 tatgatatat ccttagcttt taaccgtatc gtcatacctt tcttcgaagc tattattact
      301 tcatcctcag gatacactga ccttactcca acagcatatc cattcttatc atgtatcttc
      361 atatatgcca tacctttgcc acctcttcct ttggtcgcaa aaagggcata ctgtgttttt
      421 ttaccatacc cccgtgaggt gacaacacac aggtcagagt tttgttttac aacatccatt
      481 gcaataatct ggtcatcagt tccaacacgc ataccaatga taccagcgga atttcttccc
      541 atggcacgca tcttggcaat atttgtacgt aacagaagac cttttcttgt agcaataaca
      601 acatcatctg agccttttac aactttaacg tcaaccagct cgtcatcctt atgaagatta
      661 atagctataa tgccaccctt ttttacatta ctaaattctt ccaatgctgt ctttttcata
      721 ataccatttc tggttaccat gcataaaaag ttctggctaa aatcatccac tgaacacatt
      781 gctgtaatta tctctcctga agctaaatta ataatacctt tcaatgattt accacggtta
      841 tttttagaag caataggtat ttcataaacc ttcatcccaa atatcttgcc cttattagaa
      901 aataacaata gcatatcatg ggttgaggcg atagtcatca tagagataaa atcttctttt
      961 ctagaggaca tgcctatgac acctttcccc ccacgcccct gttttttaaa cgtatccgct
     1021 gatagccttc gaataaaacc atcattcgtg atggttatga ccatgtcttc ttcagcgatc
     1081 aggtcctcag catcaaaact taccgatgta tcttcaaaga ttatctccgt acgcctgctg
     1141 tcattatatt tagctttaat agtcagcaat tcctctttta cgattgcata tatcttcttt
     1201 tcacttttta aaatagcaga tagttcttct attaaaatat gtaattcttt taattcatct
     1261 acgacctttt ttacctccag gctggttagc ctctgtaacc gcatttctaa aatagcttta
     1321 gactgtatct ccgataattt aaaccgtttt ataagatggt cattcgcttc ttcaaccgtt
     1381 tttgatcttc taataatcgc aatcacttca tcaatattgt ctaaagctat ttttaaacct
     1441 tctaatatat gtgcccgctc ccttgccttt gccagatcat actgggtgcg tcgtgttaca
     1501 acaatctttc ggtgattgat ataatggaat atcaatcctt ttaaatccag cagttttggc
     1561 tgattattaa caagagccag gaggttaatc ccaaaggatg tctgtaactg tgtatgttta
     1621 tagagttgat tgatgataat gtttgttact gcatccttgc gacatcctat gacaatgcgt
     1681 aatcctttcc gatccgactc atcccgcagt tcattgatac cttcaattac cttattattt
     1741 accaattcag ctatttttgc aataagagct gctttgttca cctgataggg taattctgtt
     1801 acaacaatca gtgacttatt tttttgttct tcaatatgca gtttggctct aattattaca
     1861 cttccctttc ctttagcata cgctttcacc atttcttctg aaccaacaat ggtagctccg
     1921 gtagggaaat caggtccctt aacaaatttc ataagttgct taactgttgc atcaggcgta
     1981 tcaatcataa acacaattgc atctattatt tcgcctaaat tgtgtggagg aatattggtt
     2041 gccataccaa cggcaat
//
LOCUS       DAOSOX010000314         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_55952_length_2016_cov_5.803162, whole genome shotgun sequence.
ACCESSION   DAOSOX010000314 DAOSOX010000000
VERSION     DAOSOX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55952_length_2016_cov_5.803162"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLX22_12835"
     CDS             <1..827
                     /locus_tag="PLX22_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT20826.1"
                     /translation="MYINMIRVGESLGSLDSALERLADLEEKRNLLKSKVQSALWYPA
                     FMIVFAIGVVTFLMISIIPSLGRMFAELGKDLPLPTTIVMGISNFIVSFWYVLLIVTI
                     VIIYYFNRYKHTEEGRRKLDELKLKLPLVSSLYKKLLIHRFTYNLGVLLTNKVDVIKS
                     FEIVQRIIRNVLIEENIARAAKLVQEGSSIANALSRERFLPQYIIGMIAAGESSDTVD
                     EMLLNIGKIYENDIDLSINSMTSLIEPIIIVCMGFVIGLIVISVLLPIFEMNLIVK"
     gene            839..1318
                     /gene="gspG"
                     /locus_tag="PLX22_12840"
     CDS             839..1318
                     /gene="gspG"
                     /locus_tag="PLX22_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOT20827.1"
                     /translation="MGIKNSKLFFKKIIKALRDERGFTLIELLIVLTIIAILSVAVVP
                     QFINVIPSARVSAARQQIALFSTALDLYYLDNGNYPTTEQGLQALIEKPTTDPIPQNY
                     NPKGYLSKATKIPNDPWGNPYIYHCPGQHNNDYEIISYGADGKEGGTGADADITSWQ"
     gene            1332..1901
                     /locus_tag="PLX22_12845"
     CDS             1332..1901
                     /locus_tag="PLX22_12845"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOT20828.1"
                     /translation="MITFITRNNKGFTLIELIVVLAVFSILSVVVMPRIMNYLSSARD
                     NFLLLTTTIKKTFDDAFINQHTNFLIIHCDNKESEISDISSSIFERSNGISVAILNEK
                     SEFTESQNKLLKFRQFPDSFSIKEVLLTDGTIYTTGNIFIPFYPSGFSSSAIIHILIN
                     NDEEWSVTINPYLREPSVIKGYSTYEETK"
BASE COUNT          685 a          318 c          349 g          664 t
ORIGIN      
        1 agatgtatat caatatgatc agagttggag aaagtcttgg ttcattagac agcgccttgg
       61 aacgattggc agacttagag gaaaagcgta accttttaaa aagcaaagta caatctgctt
      121 tatggtatcc tgcatttatg atagtgtttg ccattggtgt tgttacattc ttaatgatca
      181 gcatcattcc ttcacttgga aggatgtttg ctgaactagg gaaagatttg cctttgccta
      241 caaccattgt tatgggtatc agcaatttca ttgtatcatt ctggtatgta ctactcatag
      301 ttactatcgt tataatatat tactttaacc gctataaaca tactgaagaa ggcagaagaa
      361 aattggatga actaaaatta aaactaccgc ttgtaagctc gttatacaaa aaactcctga
      421 tacatcgttt tacctataac ttaggtgtat tgcttaccaa caaggttgat gttataaaat
      481 catttgagat agttcagcgt attatacgaa atgttttaat agaggaaaat attgctcgtg
      541 cagctaaact ggtgcaggaa ggttcatcca ttgcaaatgc attatcacgg gaacgttttt
      601 tgcctcagta tattataggt atgatagctg ccggtgaatc aagcgataca gtagatgaaa
      661 tgcttttaaa tataggaaaa atttatgaaa atgatattga tctatctatc aatagcatga
      721 caagccttat tgaacctatc attattgttt gtatgggttt tgttattggt ttgatagtta
      781 tttcagtatt actaccaata tttgaaatga acttaattgt taaataagga ggatatgtat
      841 gggcattaaa aatagtaagt tattttttaa aaaaataata aaagctctta gagatgaaag
      901 gggttttaca ttaattgagc ttttgattgt tcttacaatc attgccattt tatcagttgc
      961 agtagttcct caatttataa atgtaattcc ctctgcacgt gttagtgctg cacgccaaca
     1021 aatagcttta ttttctacag cacttgatct ttattactta gacaatggca attacccaac
     1081 caccgaacag gggttgcagg ctttgattga aaagccaaca actgatccca tacctcaaaa
     1141 ctataatccc aaaggctatt tatcaaaagc aacaaagata cccaatgatc catggggcaa
     1201 tccctatatt tatcactgtc ctggacaaca taataatgac tacgaaatca taagttatgg
     1261 tgcagatggc aaagaaggtg gcactggtgc tgatgctgat attacaagct ggcaataagt
     1321 aatatcaatg tatgattact tttataacac gcaacaacaa aggatttaca ttaattgaac
     1381 tgattgtagt attagcagtt ttttcaatac tatctgttgt tgtgatgcca cgaataatga
     1441 attatttatc atcagcacga gataattttt tattattaac tacaacaata aaaaaaactt
     1501 ttgatgatgc atttattaat caacatacaa attttttgat aatacattgt gataataagg
     1561 agtcagaaat ttcagacatt tccagctcaa tttttgaaag aagcaatggc atttcggtgg
     1621 ctatccttaa tgaaaagtca gaatttactg aaagtcaaaa taaattgcta aaatttagac
     1681 aattccctga ttcattttct ataaaggaag tgttgctaac cgatggaaca atatatacta
     1741 ccggcaatat ttttatacct ttttatccat ccggattctc aagcagtgct atcattcata
     1801 tattgattaa taatgacgaa gagtggtcgg taaccattaa tccctattta cgtgagccat
     1861 cagtaataaa aggctatagc acctatgaag aaactaaatg atggctttac attaatagag
     1921 attcttgtcg caattgccat attgtcaata tccatgctgg ggattatgag tgctgtatca
     1981 tcaggaatct taggaattac caaaaacaaa aattta
//
LOCUS       DAOSOX010000315         1980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_57313_length_1980_cov_6.392208, whole genome shotgun sequence.
ACCESSION   DAOSOX010000315 DAOSOX010000000
VERSION     DAOSOX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57313_length_1980_cov_6.392208"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLX22_12850"
     CDS             complement(<1..835)
                     /locus_tag="PLX22_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOT20829.1"
                     /translation="METILKKIEKLMLKHDAISPELIQKKNVKLGLRNPDGSGVVAGI
                     TSKGTVIGYEKIPSGDGKVTVKPVEGKLYYCGYDACELAEKIQEEGRYGFDEITYLLL
                     TGELPDKKDLEQFSEVIADRRPLTKFERSIIMAEDVNNNQMYALHSVISHLNRCDPDP
                     DSTDIKDVIRQCINLIAKVPVIVAYNFNVMKFRKGGDLLICKPNSAWSTAENFLYLLH
                     GDGKRPNEYEAKLFDLALMLHAEHGGGNNSTFTVRTVTSSGANTYMALCAGIASLSGH
                     LH"
     gene            complement(1220..>1980)
                     /locus_tag="PLX22_12855"
     CDS             complement(1220..>1980)
                     /locus_tag="PLX22_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20830.1"
                     /translation="DFVYAASLCGKAYDMNISSAQRLKLGYSLIRDVAGGKVFILGCG
                     SPLSPAIGYVDGMRIGPDVAPYWMAPYRYYITRDPHALCTKFAIRSILTRAQLHKNLW
                     LNDPDCIMLRQKETKLTIEERMSLINSVIITGGMYFISDDLALLNNKDWKLIDIIDEL
                     VQTCHAGKTYPVDIMESKIPEIVYNTSGYIAFFNFADRPRKKIVQLKGFLKKVILPAN
                     RLVDIWGNRDIYVYSDHIDLGELKPHESLLCKIM"
BASE COUNT          642 a          393 c          285 g          660 t
ORIGIN      
        1 catgcagatg tccactgaga gatgctatac cggcacacaa tgccatgtat gtatttgccc
       61 cgcttgatgt taccgttctt accgtaaatg ttgagttatt acctccgcca tgttcagcat
      121 gaagcattaa tgctaaatca aacaattttg cttcgtattc attagggcgc ttcccatcgc
      181 cgtgtaacaa atacaaaaag ttttcagccg tgctccacgc tgaatttggt ttacaaataa
      241 gtaaatcacc accctttcta aatttcatca cattaaagtt atatgccaca ataacaggta
      301 cttttgcaat caggttgata cattgcctga taacatcctt gatatcagtt gaatctggat
      361 cgggatcaca tctgtttaaa tgtgaaatca ctgaatgaag tgcgtacatc tgattattat
      421 taacatcttc agccattatg atactgcgtt caaattttgt taaaggacgt ctatcggcaa
      481 tcacctcgct aaattgctcc agatctttct tatctggcag ttctccagta aggagtagat
      541 aggtaatttc atcaaaacca tatctacctt cttcctgaat tttttcagct aattcgcagg
      601 catcatagcc gcaataatat aacttcccct caactggttt aacagtaact ttgccatcac
      661 ctgaaggtat tttttcatat ccaataactg taccttttga tgtaatacca gcaacaaccc
      721 cagagccatc tggattacgt aatcccaatt tgacattttt tttctgaata agctccgggc
      781 ttatcgcatc atgcttcaac ataagttttt caattttttt aagtatggtt tccattagca
      841 cctcactggt ataagtataa aattgttctt ttttgttgcc agtatcttca attttcttct
      901 atatggacta tgtttctaag tatacaatat tttgtataat catctatttt taatattaaa
      961 ttaatccaat tattttacag taatgtaatg ctaaagaggt aaatcaagta tgaagggatt
     1021 attcaaagat ataaataata atttgttagt agcttaggat aagtagaata tctaaaaact
     1081 aaatattgta ataatgcagt gagcacataa ttattgataa aatgagttaa gaaaaactgg
     1141 aaaaataata aataatcttt agagtcgcaa tatattctgc tgtaaaaact actgctgtta
     1201 ttttttacaa gtacaacatt tacattattt tacacaataa tgattcatga ggtttgagtt
     1261 cacctaaatc aatatgatcg ctataaacgt aaatatctct gttaccccat atatcaacaa
     1321 gcctgttagc aggcaaaatc acctttttta aaaaaccctt taactgtaca attttttttc
     1381 taggcctatc agcaaaattg aaaaaggcta tatatcctga agtattataa acaatttctg
     1441 gaatcttgct ttccataata tccaccggat atgttttacc agcatgacat gtctgaacaa
     1501 gctcatcaat aatatcaata agcttccaat ctttattgtt taataatgct aaatcatcag
     1561 aaataaaata catgccacca gtaattatta ccgaattgat aagggacatt cgctcttcta
     1621 ttgttaattt tgtttctttt tgccttaaca ttatacaatc gggatcgtta agccataaat
     1681 ttttatgcaa ctgagccctg gttagtatgc tccgtattgc aaatttggta cacaatgcat
     1741 ggggatctct ggtaatataa taccggtatg gagccatcca gtacggcgct acatcaggac
     1801 caatacgcat cccatcaaca taaccaattg ccggtgataa aggcgaacca caccctaaaa
     1861 taaatacctt tccccctgca acatctctta tcaaactata ccctaacttt aatcgttgcg
     1921 ctgaggaaat attcatatca tacgctttcc cgcataatga cgcagcataa acaaaatcta
//
LOCUS       DAOSOX010000316         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_57597_length_1973_cov_11.030761, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000316 DAOSOX010000000
VERSION     DAOSOX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57597_length_1973_cov_11.030761"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..459)
                     /locus_tag="PLX22_12860"
     CDS             complement(28..459)
                     /locus_tag="PLX22_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOT20831.1"
                     /translation="MNVTQLSLFLENKPGHLLNALKVIADENINILTLTIAEVSDFGI
                     VRMIVTKPQKAIEALRSHKFVCSPTDVLAIELDDKPGSLLQALEIFAKRNLNIEYMYA
                     FTEKRGDKAVMIFKFSDIETAKKSLLEEGFNVIKKIDLIGE"
     gene            complement(567..1868)
                     /locus_tag="PLX22_12865"
     CDS             complement(567..1868)
                     /locus_tag="PLX22_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT20832.1"
                     /translation="MFNREYESMEVKKLKELQLERLRWSLKHAYNNVAHYKNAFDKAR
                     FNPDKFSSLDDLKHVPFLMKQTMRDSYPYGLFAVPLEKVIRIHSSSGTTGNATVAGYT
                     RNDIEVWAELIARSIVGAGGSEKDIVQDAYGYGLFTGGLGLHYGAEKLGATVIPVSAG
                     NTERQLMLIKDFGTTILVCTPSYAVNMADYAEKKMPDYDFKKTKLKAGIFGAEPWTQS
                     MRKEIENRMGIDAYDIYGLSEVMGPGVSCECSQKSGLHIFEDHFYVEVIDPETGEVLP
                     EGEQGEIVYTSLTKEACPVIRYRSRDITRIYRDSCKCGRTMIKMEKVTGRSDDMLIIR
                     GVNVFPMQIEAVLMEVEGTEPHYQIIVDRKGALDDVEIKVEVNEKIFTDQLGVLKGIT
                     ERIEQRIRSVLGISAKITLVEPSTLQRSEGKAKRVIDKRNL"
BASE COUNT          580 a          447 c          275 g          671 t
ORIGIN      
        1 cccagcataa cttttttttg cattgcatta ctccccaatt aaatcaattt tttttatcac
       61 attaaatccc tcttcaagaa gcgatttttt tgctgtttca atatctgaaa acttaaaaat
      121 cataacagcc ttatcaccac gtttctcagt aaatgcatac atatattcaa tatttaaatt
      181 tcgtttagca aatatttcaa gtgcttgtag caatgaacct ggtttatcat ctaattcaat
      241 agccagtaca tcggttgggg aacaaacaaa tttatgagaa cgcaaagctt ctattgcttt
      301 ttgaggttta gtaactatca tccgtacaat accaaaatct gatacttctg caatagttag
      361 cgtaaggata ttaatatttt catctgctat tacttttaat gcattcagta ggtgtcctgg
      421 cttattctcc aaaaaaagtg ataattgcgt tacattcatg gttattctcc taaaaataaa
      481 tttacgtggg cacaccacat tgagttaatt ctacgaatat tttttgcttt atgaatgaga
      541 tcctgcatca tttacatctt tattctctac aaattacgtt tgtcaataac gcgttttgct
      601 ttaccttcac tacgctgcaa ggtacttggt tctaccaatg taatcttagc actgattccc
      661 aatacgcttc tgatacgctg ttcaatccgc tcagttattc cctttaatac acccagttga
      721 tcggtgaata ttttttcatt tacctccacc tttatttcaa catcatcaag agcacctttg
      781 cgatccacga taatctggta atgaggttca gtaccttcaa cttccatgag gactgcttct
      841 atctgcatgg ggaatacatt aacgcccctg atgataagca tatcatcact tcttcccgta
      901 accttttcca ttttaatcat tgtcctacca cacttacagg agtcccgata tattctggta
      961 atgtctcgtg acctataacg aataaccggg catgcctctt tcgtaagtga agtatatact
     1021 atttcaccct gttcgccttc tggcaatacc tctcctgttt cagggtctat tacctcaaca
     1081 taaaaatggt cttcaaatat atgcaatcct gacttctggc tgcattcgca tgatacgcca
     1141 ggacccatga cctcacttaa tccatatata tcatatgcat caattcccat acgattttct
     1201 atctcttttc gcatactttg agtccatggt tcagctccaa aaattcctgc tttaagcttt
     1261 gtttttttaa aatcataatc aggcattttc ttctcagcat aatctgccat attaactgca
     1321 tacgaaggag tacaaacgag gatagttgta ccaaaatcct ttatcaacat caactgtcgc
     1381 tcggtattgc ctgctgatac cggtataaca gttgcaccca gcttttcagc accataatgc
     1441 aatcccaatc cgccagtaaa caatccataa ccataagcat cctgaactat atccttttct
     1501 gaacctcctg caccaactat ggatcgagct atcagctcag cccacacctc aatatcattt
     1561 cttgtatacc cggctacggt tgcattcccc gtagtacctg atgaagaatg tatccgaata
     1621 accttttcca atggcactgc aaataagcca taaggatagc tatctcgcat cgtctgcttc
     1681 atgagaaaag gaacatgctt caaatcatca agagaggaaa acttatcggg attaaacctt
     1741 gccttatcaa aagcattttt ataatgggca acattattat aggcatgctt aagcgaccat
     1801 cgtaaccgct ccaattgaag ttcttttaac ttttttactt ccattgattc atattctcta
     1861 ttgaacattg ccaactcctt ttatgtggct atctctattt aaaattttta agattgtaga
     1921 ttttattaat aattattaaa attgtcaata aacttgcaaa tgtttaaaaa aaa
//
LOCUS       DAOSOX010000317         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_57599_length_1973_cov_6.698123, whole genome shotgun sequence.
ACCESSION   DAOSOX010000317 DAOSOX010000000
VERSION     DAOSOX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57599_length_1973_cov_6.698123"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLX22_12870"
     CDS             complement(<1..835)
                     /locus_tag="PLX22_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOT20833.1"
                     /translation="METILKKIEKLMVQHDAISPELIQKKNVKLGLRNPDGSGVVAGI
                     TSKGTVIGYEKIPSGDGKVTVKPVEGKLYYCGYDACELAEKIQEEGRYGFDEITYLLL
                     TGELPDKKDLEQFSEVIADRRPLTKFERSIIMAEDVNNNQMYALHSVISHLNRCDPDP
                     DSTDIKDVIRQCINLIAKVPVIVAYNYNVMKFRKGGDLIICKPNAAWSTAENFLYLLH
                     GDGERPNEYEAKLFDLALMLHAEHGGGNNSTFTVRTVTSSGANTYMALCAGIASLSGH
                     LH"
     gene            complement(1213..>1973)
                     /locus_tag="PLX22_12875"
     CDS             complement(1213..>1973)
                     /locus_tag="PLX22_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20834.1"
                     /translation="DFVYAASLCGKAYDMNISSAQRLKLGYSLIRDVAGEKVFILGCG
                     SPLSPAIGYVDGMRIGPDVAPYWMAPYRYYITRDPHALCTKFAIRSILTRSQLHKNLW
                     LNDPDCIMLRQKETKLTIEERMSLINAVVITGGMYFISDDLALLNNTDWKLIDIIDEL
                     VQTCHTGKTYPVDIMESKIPEIVYNTSGYIAFFNFTDRPRKKIVQLKGLLKKVVLPAN
                     RLVDIWGTRDIYVYSDHIDLGELKPHESLLCKIM"
BASE COUNT          643 a          390 c          274 g          666 t
ORIGIN      
        1 catgcagatg tccactgaga gatgctatac cggcacacaa tgccatgtat gtattggcac
       61 cgcttgatgt taccgttctg acagtaaatg ttgagttatt acctccgcca tgttcggcat
      121 gaagcattaa tgctaaatca aacaattttg cttcatattc attagggcgc tccccatcgc
      181 cgtgcaacaa atacaaaaag ttttcagccg tgctccacgc tgcatttggt ttacaaataa
      241 ttaaatcacc accctttcta aatttcatca cattatagtt atatgccaca ataacaggta
      301 cttttgcaat caggttgata cattgtctga taacatcctt gatatcagtt gaatctggat
      361 cgggatcaca tctgtttaaa tgtgaaatca ctgaatgaag tgcgtacatc tgattattat
      421 taacatcttc agccattatg atactgcgtt caaattttgt taaaggacgt ctatcggcaa
      481 tcacctcgct aaattgctcc agatctttct tatctggcag ttctccagta aggagtagat
      541 aggtaatttc atcaaaacca tatctacctt cttcctgaat tttttcagct aattcgcagg
      601 catcatagcc gcaataatat aacttcccct caactggttt aacagtaact ttgccatcac
      661 ctgaaggtat tttttcatat ccaataactg taccttttga tgtaatacca gcaacaaccc
      721 ctgagccatc tggattacgt aatcccaatt ttacattttt tttctgaata agctccgggc
      781 ttatcgcatc atgttgcacc ataagttttt caattttttt aagtatagtt tccattagca
      841 cctcactgtt ataagtataa aattgttttt tcttgttgcc agtatcttca attttcgatt
      901 atatgaacta tgtttctaag tatacaatat tttgcataat catctatttt taatattaaa
      961 ttaatctaat aattttacag taacgtaatt aaaaagaggc aattcaagca tggaaggaat
     1021 tttaaaagat ataaatacta tttttatata actttacgat aagtaaaaca tctaaaaaat
     1081 aaatttttaa taatgttgtg agcacataat tattaaaaaa taagttaaaa aaaactggaa
     1141 aaataaaaaa taatcttcag agttgtaata tattctgctg taaaaccatt tctgttttta
     1201 caaatataac atttacatta ttttacacaa taatgattca tgaggtttaa gttcacctaa
     1261 atctatatga tcgctataga cgtaaatatc cctggtaccc catatatcaa caagcctgtt
     1321 agcaggcaaa actacttttt ttaacaaacc ctttaactgc acaatttttt ttctaggcct
     1381 atcagtaaaa ttgaaaaaag ctatatatcc tgaagtatta taaacaattt ctggaatttt
     1441 actttccatt atatctaccg gatatgtttt gccagtatga catgtctgaa caagctcatc
     1501 aataatatca ataagcttcc aatcggtatt gtttaataat gctaaatcgt cagaaataaa
     1561 atacattcca cccgtaatta ctaccgcatt gataagagac atccgctctt ctattgtcaa
     1621 ttttgtttct ttttgtcgta gcattataca atcagggtcg ttaagccata gatttttatg
     1681 caactgtgat ctggtcagta tgctccgtat tgcaaatttg gtacataatg catgtggatc
     1741 tctggtaata taatacctgt atggagccat ccagtacggc gctacatccg gaccaatacg
     1801 cattccatca acataaccaa ttgccggtga taaaggagaa ccgcacccta aaataaatac
     1861 cttttctcct gcaacatctc ttatcaaact ataccctaac tttaatcgct gcgctgaaga
     1921 aatattcata tcatacgctt tcccgcataa tgacgcagca taaacaaaat cta
//
LOCUS       DAOSOX010000318         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_57917_length_1966_cov_6.604919, whole genome shotgun sequence.
ACCESSION   DAOSOX010000318 DAOSOX010000000
VERSION     DAOSOX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57917_length_1966_cov_6.604919"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLX22_12880"
     CDS             complement(<1..273)
                     /locus_tag="PLX22_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT20835.1"
                     /translation="MKEGYVVPTIGKIAYGLGDLAINIAYTTIGFYLLFFLVNVAGIP
                     AQWAGLIFFIARAWDAITDPMMGMISDRTKSRFGRRRVYFLFGCIPF"
     gene            complement(620..1411)
                     /gene="arsM"
                     /locus_tag="PLX22_12885"
     CDS             complement(620..1411)
                     /gene="arsM"
                     /locus_tag="PLX22_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOT20836.1"
                     /translation="MDSPIKIKNIVKQNYGKIALSKTSCCVTGSCCSSADVAKNAGYS
                     ADELAKLDDANLGLGCGNPTALSSLKAGEIVLDLGSGAGIDCFIAAKKVGDSGRVIGI
                     DMTPEMIQLAKENAKKLKFHNVEFILGDIEHIPIEDNIVDVVISNCVINLTSDKEQVL
                     KEVYRVLKNGGRMAISDIVLNGTLPEEIKNSELAYAGCIAGAVQKEYYISLIYNVGFH
                     DVTIVKECAYNTIFESKELKVDDVHVSNFKNYSDVIKSISIVAWK"
BASE COUNT          662 a          386 c          283 g          635 t
ORIGIN      
        1 aaatggaata cacccaaaca aaaaatacac acgtcgtcta ccaaaccttg attttgttct
       61 gtcagatatc atccccatca taggatcggt gatagcatcc cacgcccgtg caataaagaa
      121 gatcaaacct gcccactgtg caggaattcc ggcaacatta accagaaaaa ataaaagata
      181 aaatccaatt gttgtatatg caatattgat ggctaaatcg cccaatccat aggcaatctt
      241 tcctatggtg ggaactacat atccttcttt catggcttac catccagaat aaataacaat
      301 agtgattaaa taaaaaaaca tattgtaagg aattagatta tacctatggt taaaatgcaa
      361 ggaaatttat gataatagat agtataaatt attaatgctt tttataggat tgaataataa
      421 ctttgaatcc ttattttgca gttgggtcac aatagaatgc atcatcagaa taaaaagtta
      481 ttaatatttt tggttgattc cctgtccatg aattcagcca attttcacaa aattttgaag
      541 cttcatctac gtgcatttgc ccctccaatt aaaatacgtt aagaaatata gcaaccatgc
      601 tgatacaatc tatgatacac tatttccatg ctacaatact tatgcttttg attacatcgc
      661 tataattctt aaaatttgat acatgtacat catctacttt taattcttta ctttcaaaaa
      721 tagtattgta agcacattct ttgacaatgg taacatcatg aaatcccaca ttatatatca
      781 atgagatata atactccttt tgaacggcac cggcaataca tcccgcatat gctaattctg
      841 agtttttaat ctcttccggt agtgtaccat tcaatacaat atcagatatt gccattctac
      901 caccattttt taacacacgg tatacttctt taagtacctg ctctttatct gatgttaaat
      961 tgattacgca attggatata actacatcaa cgatattatc ttcaatcggt atatgctcaa
     1021 tatctcccaa tataaattct acattatgaa atttaagttt ttttgcattt tcctttgcta
     1081 attgtatcat ctcaggtgtc atatcaatac cgatgactct accactgtcg cctactttct
     1141 ttgctgctat gaaacaatca atgccagcac cgcttcctaa atcaagaaca atctcaccgg
     1201 ctttcagtga agataaagcg gttggattac cacatccaag ccctaaattg gcatcatcaa
     1261 gtttagcaag ttcatcagca gaataccctg catttttagc aacatcagca gatgaacaac
     1321 agctaccggt aacacaacat gaagttttag ataatgcaat cttcccataa ttttgcttaa
     1381 caatgttttt tatttttata ggtgaatcca cagtaagcct ccttttcaac taactattac
     1441 ctgtaaatat attcaattac ttgaataacg ttgcaatctg ttccgcaaca atggaataaa
     1501 aaatggaata aaacgaatct gcctttataa atgccataca acacaataat acatcccttt
     1561 gccaaattgc aataataaat ttataatctt gcctttttgt tttttcgctt gaaatgttaa
     1621 tacaggtcag agatagtgcc tgtgttatct gacccacagg gatgacataa cataatagtt
     1681 tgataaagat tatgtttcca ttcataaata ttgccggtgc acgagacagc ttttttaccg
     1741 tgattcattt catgatgtta catttagtat gctgtatgcc agtagaggta tcagttttac
     1801 cagaaacttg aagatgggga gatttcaggc tacctttgcg cagaacacac aaagggaggt
     1861 gaataattat tatgagactc cagcaaatag aacccaaatc acatcttgca catggtgaat
     1921 accagcacag gtatctttta gcaacggata ttgtaacaat atgtgt
//
LOCUS       DAOSOX010000319         1951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_58549_length_1951_cov_6.389768, whole genome shotgun sequence.
ACCESSION   DAOSOX010000319 DAOSOX010000000
VERSION     DAOSOX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58549_length_1951_cov_6.389768"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..643)
                     /locus_tag="PLX22_12890"
     CDS             complement(461..643)
                     /locus_tag="PLX22_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20837.1"
                     /translation="MFYCLKFTPQPEASMMLITGENEHFLSKHNTDMVDAWLDHVYFE
                     LGKEEVSYSMKLIPEK"
     gene            complement(807..>1951)
                     /gene="metK"
                     /locus_tag="PLX22_12895"
     CDS             complement(807..>1951)
                     /gene="metK"
                     /locus_tag="PLX22_12895"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT20838.1"
                     /translation="DYLFTSESVSEGHPDKVADQISDAILDAHIQGDPYSRVACETLV
                     TTNRVIISGEITSTVTVDYEAVARKVIASIGYTSPEIGFDAKACNVEVYIHPQSPDIS
                     QGVTEGQGLFKEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLADIRKKGEINF
                     LRPDGKSQVTVEYQNDKPKRISTIIVSTQHDAAVTLPTLEEMVRELVIKNVIPSNLWD
                     KNTKIYVNPTGRFEIGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDRSAA
                     YMGRCIAKTIVAAGLASRCELQVAYAIGVADPVSVMVDTFGTNNIPEETIEKRIQHVF
                     NLKPKSIIQTLDLLKPKYTITAAYGHFGRNEPGFTWEQTDKAQELR"
BASE COUNT          580 a          416 c          344 g          611 t
ORIGIN      
        1 cgttgtattg tccatataca acctgcatat cgatattcat attgtgcaag ctctgggcat
       61 cattccgagc accatcctga gcaaacagaa aagaaacaaa caccataccc tgtagtataa
      121 gtgtcatgaa tatataaagg caccttttgc tattatttat atttcccatg caaccccata
      181 ctgtatatgt aattcattaa ggatgaataa tatcataaaa atggaatgta atgaaggttg
      241 attatttata cagggatatt ttaagtcaat acaaatatga ataagtttta atacgtacac
      301 ctatttcatc aataatttta ttgtaataac catgcatttt ccattgaaac tttattgtaa
      361 tgcttcatta gctcaactat ggtcagcatc attatctctt aacacaataa ctgccaataa
      421 gctattttgc tgccacaagc actaaatgca cttcaatggg ttatttctca gggatcaatt
      481 tcatagaata ggacacctct tccttaccca gttcaaaata tacatgatca agccatgcat
      541 caaccatatc agtattatgc ttacttaaaa agtgttcatt ttctcctgta atgagcatca
      601 tggatgcttc aggttgtgga gtaaacttta gacaatagaa catgcaatcc cctctttttt
      661 acagcgataa gcgatctcat ggcttgctga aaaaaaatag ctgttatcac aataaaatca
      721 taccatgtca atgtttcaag tctcaataaa aaaagcgtgg aaatccacgc ttttgttgta
      781 tcatcttttt atagaaacag attgtatcat cgcaattcct gggctttatc agtttgttcc
      841 catgtaaaac caggttcatt gcgcccaaaa tgtccgtacg ctgcagttat tgtatacttt
      901 ggttttaaca gatcaagcgt ttgtatgatt gattttggtt ttaaattgaa gacatgttgt
      961 atgcgttttt caattgtttc ttctggtatg ttgtttgtac caaaggtatc aaccataaca
     1021 gaaacaggat cagcaacccc tatggcatat gccacctgaa gttcacatcg cgatgcaagc
     1081 cctgctgcaa caatagtttt tgcaatgcag cgtcccatat aggcggcaga gcggtcaacc
     1141 tttgacggat ccttgcctga aaatgctccg ccaccatgtc tgcccattcc accgtaacta
     1201 tcgacaataa tcttcctgcc ggttaatccg gtatcaccat ggggaccgcc aatctcaaaa
     1261 cgccccgttg ggttgacata aatttttgtg tttttatccc agaggttcga aggtataaca
     1321 tttttaataa caagctcacg caccatctct tcaagtgttg gcagcgtaac agcagcatca
     1381 tgctgtgtag aaacaatgat agtgcttata cgttttggtt tgtcattttg atattcaacg
     1441 gttacctgag atttgccgtc agggcgtaaa aaatttatct cacctttttt tctgatatct
     1501 gcaaggcgtt ttaccagcct gtgtgcatat aaaataggca tgggcataag ctcatcggtt
     1561 tcagctactg catagccaaa catcatcccc tggtcgccag caccctgctc tttgaaaagc
     1621 ccctgtcctt cggtaacacc ctgtgaaata tcgggtgatt gaggatgtat atatacttct
     1681 acattgcatg cttttgcatc aaaaccaatc tcaggtgaag tatatccaat gctggcaatt
     1741 accttacggg caacagcttc ataatcaaca gtcacagtcg atgtaatttc gccggaaatg
     1801 ataacccggt ttgtggttac aagcgtttca cacgcaacac gagaatatgg atcaccctgt
     1861 atgtgtgcat ctaaaattgc atcggatatc tggtcggcaa ccttatcagg atgtccttct
     1921 gaaactgatt cagaagtaaa taaatagtct t
//
LOCUS       DAOSOX010000320         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_58626_length_1949_cov_8.725977, whole genome shotgun sequence.
ACCESSION   DAOSOX010000320 DAOSOX010000000
VERSION     DAOSOX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58626_length_1949_cov_8.725977"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1949)
                     /locus_tag="PLX22_12900"
     CDS             complement(<1..>1949)
                     /locus_tag="PLX22_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOT20839.1"
                     /translation="PWEFKTGKGIFSSPVVDEDGTVYIGSADHYFYAITQNGSLKWKC
                     KTNEIIDSSALLDNRGFVYVGSGDSYVYCINRETGRIVWKSKAHAVQEVQQQFKIKTY
                     NVNWFEGNIGMLPDGSIIAPNDNYLVYELDRATGQRKTQYIANEMIWSLPAVNIKTER
                     LFFGTQFMALKNIYCYNYRTKELEWTNGGWGSNAASPLLTSSAENGLVIVGGYDGYVR
                     AFAQDSGKQLWKTGLRDHIYASPAQLSNGLILQPSADGTIYALNPDNGSIVWAYDTLE
                     PIRSSPAIDGNDFIYVGSGDGRLYCLHPDGTLRWSYLCIDEDRNDLNSSPALGKEGIY
                     IGGENGSVFFVPYDYPVHNKSRDKRCMVMQGEMLPDEGAYLVYTNNFGKLFKQPPAMI
                     DANQPLAFTLFVRNNSNTIKSAIDRDTLDLKVTGNPSFKVFVSANKQFFILVPQEVWS
                     GKKTSTMSVRIKGNYISNMRRFGLKFFGGSRGGNFDVTYSFAIAPHTGTMPYTIPLKA
                     GNKQTVFELKRLSAPNPTMLPSWNQIGFDSLHYIGGIVCNSNNAMIVWVIGGKLKDGK
                     TVVDPALEARYPLYLLYDNGLLTLYNYDGFKINFIGSWDMPFGQYRLSTKVDPITGAI
                     ARAPELFAIALGDEIQFYGRFLKLMG"
BASE COUNT          561 a          446 c          369 g          573 t
ORIGIN      
        1 cccatgagtt ttaaaaatct gccataaaat tgaatctcat cacccaatgc aatggcaaaa
       61 agttcaggtg ctctggcgat agctccggta atgggatcta cttttgtgga aagcctgtat
      121 tgtccaaatg gcatatccca tgagcctatg aaattgattt taaagccatc atagttgtac
      181 agtgttaaga gaccattatc gtataaaaga tacagcggat atcgtgcttc aagtgctgga
      241 tcaacgacgg ttttaccatc cttaagcttt ccaccaatta cccagacaat catggcattg
      301 ttactattgc aaacaatccc gcctatatag tgcaatgaat caaaaccaat ctgattccat
      361 gaaggaagca tggttggatt aggtgctgaa agccgtttca attcaaatac cgtttgttta
      421 tttcctgctt taagcggtat tgtatacggc attgtaccag tgtgaggagc tatggcaaaa
      481 gaataggtta catcaaaatt tccacctcgg gaaccaccaa agaatttaag gccaaacctg
      541 cgcatattgc ttatatagtt tccttttatg cgcacactca tagtgctggt ttttttgccg
      601 ctccacacct cctgtggcac aaggataaaa aactgcttgt tggcagaaac aaaaacctta
      661 aatgaaggat ttcccgtaac ttttaaatcc agcgtatcac ggtcaatggc tgattttatt
      721 gtgttgctat tgttccgaac aaatagtgta aatgctaacg gttgattggc atcaatcatt
      781 gctggcggct gtttaaaaag ttttccaaaa ttatttgtgt acacaagata tgcaccttca
      841 tcaggaagca tttccccttg cattaccata caccgcttat ccctgctttt attatgcacc
      901 ggataatcat agggaacaaa gaagacactc ccattttcac caccaatgta gatgccttct
      961 ttgcctaaag caggggaact gtttaagtca ttcctgtctt catcaatgca tagatatgac
     1021 cagcgcaatg tgccatctgg atgtaaacaa tacaacctgc catcaccgga accaacatat
     1081 ataaaatcat tgccgtcaat tgcgggcgat gaacggattg gttcaagcgt atcgtaagcc
     1141 cacacaatag aaccattatc aggattgagg gcatagatag tgccatctgc tgatggctgt
     1201 agtatcaaac catttgaaag ctgagcaggg cttgcatata tgtgatccct cagtccggtt
     1261 ttccatagct gtttcccact atcctgcgca aatgctcgca catagccatc ataaccacca
     1321 actatcacca gaccattttc agcactgctg gttaaaagcg gtgaagcagc attggaaccc
     1381 cagccaccgt tggtccattc caattctttt gtcctgtagt tatagcaata gatattcttc
     1441 agtgccataa actgtgtgcc aaagaaaagc ctttctgttt ttatgttcac agcagggagc
     1501 gaccatatca tctcattggc tatgtactgt gtttttcttt gtcctgttgc tctgtcaagt
     1561 tcgtacacca ggtagttgtc gttgggagca ataatactgc cgtcgggaag catgccaatg
     1621 tttccttcaa accagttaac attataggtt ttgatcttaa attgttgctg aacttcctgc
     1681 accgcatgtg cctttgattt ccagacaatc ctgcctgttt cacggttgat acaataaaca
     1741 taactatcgc ccgaaccaac atacacaaaa cccctgttgt ccagaagagc ggatgagtca
     1801 atgatctcat tggttttaca tttccacttc aatgaaccat tttgcgtaat tgcataaaaa
     1861 taatggtcag ccgaacctat atacaccgtg ccatcttcat caacaacagg cgagctgaaa
     1921 atacctttgc ctgttttaaa ttcccatgg
//
LOCUS       DAOSOX010000321         1947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_58712_length_1947_cov_8.231501, whole genome shotgun sequence.
ACCESSION   DAOSOX010000321 DAOSOX010000000
VERSION     DAOSOX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58712_length_1947_cov_8.231501"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLX22_12905"
     CDS             complement(<1..284)
                     /locus_tag="PLX22_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT20840.1"
                     /translation="MINVQNLVKHYGDVKAVDGISFTIRKGEVTGLLGPNGAGKTTTL
                     RILTCYLQPTSGMVSIDDYLVDENPIEIKKRIGYLPESAPLYPDMMVYDF"
     gene            complement(533..889)
                     /locus_tag="PLX22_12910"
     CDS             complement(533..889)
                     /locus_tag="PLX22_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOT20841.1"
                     /translation="MDERTVYSEHRPWGFFEVLSDTETHKVKRITVYPHKRLSLQRHA
                     RRKEHWFFIHGRGVVTLDTDNIPVDVGKCVDIDFNVVHRVENTGDENVVYIEVQLGEY
                     FGEDDIERIEDDYGRI"
     gene            complement(895..>1947)
                     /gene="serC"
                     /locus_tag="PLX22_12915"
     CDS             complement(895..>1947)
                     /gene="serC"
                     /locus_tag="PLX22_12915"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOT20842.1"
                     /translation="SILPVEVLEEASRQLVDYRNTGMSLIEMSHRGKIYDEVHNEAIV
                     LVREMLQVPDDYDILFIQGGATLQFGMVPMALLMADSSADFVITGTWAKKAYQDARLI
                     GNARIIWDGAGEKYRRIPSIDELSLHDDAVYVHICSNETIDGIQWQQLPDTGNTPLIV
                     DMSSDIMSRAVDWNKVSMLFAGTQKNLAPAGMALVIMKKSLVEKARKDIPAYLRYDLH
                     AKENSLYNTPPTFTVWMTNLTLHWIKGIGGLPEIEKRRDRKAAMLYDAIDNSGGYYTS
                     PVDKNSRSAMNVVWHLPDEQLENKFVDDAEKEGLSGLKGHRSVGGLRASLYNAMPVEG
                     VQTLVEFMHRFMEKHG"
BASE COUNT          555 a          464 c          340 g          588 t
ORIGIN      
        1 aaaaagtcat ataccatcat atcaggataa agcggagccg attcgggaag atagccaata
       61 cgctttttta tctcaattgg attttcatcc accagataat catcgatact taccattccc
      121 gatgttggtt gaagatagca ggttaaaatc cgcaacgtgg tggttttacc ggcaccgttg
      181 ggacctaaaa gtccagtaac ctcaccttta cgaatggtaa atgatatgcc gtcaactgct
      241 ttcacatcgc cataatgttt aacaagattc tgtacgttta tcatgcaggg cattcctcct
      301 gaaaatagtt aatcatcagt tttatcctga agcgatagct gctgttaatg ccacctgatg
      361 attataataa aagtttttat tgcaaaggtc atttttaaaa gcttcaataa ccttttatga
      421 taaagttatc atgattattc aaaaacatta cattgtaaca ttgtattgta atattaagct
      481 caagctttgt atgtagtatg aaaaaatcaa ttataatttg ttacacacta ttttatatac
      541 gcccataatc atcctcaata cgctctatat catcttcacc aaaatattca ccaagctgaa
      601 cctctatata caccacattt tcatcaccgg tattttccac ccggtgcact acattaaaat
      661 ctatatcaac acatttcccg acatcaacgg ggatgttatc tgtatcaagg gttaccaccc
      721 ccctgccatg tataaaaaac caatgctctt tgcgccgtgc atgacgctga aggcttagcc
      781 gtttatgggg atacacggtt atccgtttta ccttgtgagt ttcagtgtcc gatagtacct
      841 caaaaaaacc ccatggcctg tgctcagaat atacagtacg ttcatccata ataatcaccc
      901 atgtttttcc ataaagcgat gcataaattc aaccagggtt tgcacccctt ctacaggcat
      961 tgcattgtat agagaggcac gtaatcctcc taccgagcgg tgacccttta atccgcttaa
     1021 accttctttt tcggcatcat cgacaaattt attttctaat tgctcatcgg gcagatgcca
     1081 cacaacattc atggcagaac ggctgttttt atcaacagga ctggtatagt atccgccact
     1141 gttgtcaatt gcatcataga gcatggcagc tttccggtca cgccgttttt ctatctccgg
     1201 caatccccct attcccttaa tccagtgcaa ggtaaggttg gtcatccata cggtaaatgt
     1261 gggaggggtg ttgtaaagtg aattttcctt tgcatgcaga tcataccgta aataggcagg
     1321 tatgtctttg cgggcttttt ctaccaatga ttttttcata atcacaagcg ccatccctgc
     1381 tggagctaaa ttcttctgtg tgcccgcaaa gagcatggaa accttattcc aatcaaccgc
     1441 tcgtgacatg atatcagatg acatatcaac aatcaagggg gtgttccccg tatccggaag
     1501 ctgttgccac tgtatgccat ctatcgtttc attggaacat atatgtacat atacagcatc
     1561 atcatgcaat gacaattcat caatggaagg gatacgtcga tatttttcac cagcgccatc
     1621 ccagattatt cgtgcattcc caataagcct tgcatcctgg tatgcttttt ttgcccatgt
     1681 accggttata acaaaatctg cactgctgtc agccataagc aatgccatgg gcaccatacc
     1741 aaactgcagt gttgcgccgc cctgaataaa caaaatatcg tagtcatcgg gcacctgtag
     1801 catttccctg acaagaacta tcgcttcatt atgcacctca tcatagattt tacctctatg
     1861 gctcatctct ataagcgaca tcccggtatt tctataatca acaagctggc gtgaagcttc
     1921 ttccaatacc tcaactggca atatcga
//
LOCUS       DAOSOX010000322         1942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_58927_length_1942_cov_7.643349, whole genome shotgun sequence.
ACCESSION   DAOSOX010000322 DAOSOX010000000
VERSION     DAOSOX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58927_length_1942_cov_7.643349"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLX22_12920"
     CDS             complement(<1..926)
                     /locus_tag="PLX22_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT20843.1"
                     /translation="MEISTVLNQILMAAYNEAKNRKHEYLTPEHVLLASLYFPEGREV
                     IEGCGGNVEQLKRNLSKHLSMHIPVIADGEPSQSVGLQHVIERAIMHINSAQKEILDF
                     GDIVIAIYEEEESFASYFLAREGITRYDLLNYISHGGSFEEEEIEEFGDEVGGNTEKQ
                     SKVLEQFTIELTQKAQKGELDPLIGREDILERTMQVLSRRLKNNPVHVGEPGVGKTAI
                     TEGLAQRIVAGAVPQSLKNAKIYRLDMGGLIAGTRFRGDFEERLKRVIAELQKQEKVI
                     LFIDEIHTIVGAGAVSGGSMDASNILKPVLADG"
     gene            complement(978..1289)
                     /gene="clpS"
                     /locus_tag="PLX22_12925"
     CDS             complement(978..1289)
                     /gene="clpS"
                     /locus_tag="PLX22_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOT20844.1"
                     /translation="MAHRYEYDGNLQIKEDVQVKKPRMYKVILHNDHYTTMDFVVEVL
                     VTIFHKNNMEATELMLQVHRNGFGICGVYTYDIAQTKAGQVHTLARQREFPLKCTVEE
                     A"
     gene            complement(1403..>1942)
                     /locus_tag="PLX22_12930"
     CDS             complement(1403..>1942)
                     /locus_tag="PLX22_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOT20845.1"
                     /translation="NIKIPVNPAMQPDEQCHFDGINAVKVLQSEGIPVNCTLVFTPEQ
                     ALLAAKAGAAYISPFAGRIDDYIRSSAHIKFDKSDYFPEEGIEQNGVLLHDNGIVSGI
                     DVVSQCVEIVTYYEYKSEIIAASLRNPRQVREAALAGAHIATVPFGVIAQMLRHEKTF
                     EGMKKFTDDIVPEYEKLIG"
BASE COUNT          544 a          474 c          313 g          611 t
ORIGIN      
        1 ccatcggcta acaccggttt taaaatattg gaagcatcca ttgaaccgcc gctcactgca
       61 cccgcaccaa cgattgtatg tatctcatca ataaacagta tcaccttttc ctgcttttgc
      121 aattcagcaa tcacacgctt aaggcgctct tcaaaatcac ctcggaatcg tgtaccggca
      181 ataagcccac ccatatccag ccggtagatc tttgcatttt tcagtgattg cggcacagca
      241 cctgccacaa tgcgctgagc caatccctcg gtaattgcag ttttaccaac accaggttca
      301 ccgacatgga cagggttgtt ttttaatctt cgcgacagca cctgcatggt acgttctaaa
      361 atatcctccc tgccaataag gggatcgagc tctccctttt gcgctttttg cgttaattca
      421 atagtaaatt gctccagcac tttgctttgt ttttcggtat tacccccaac ctcatcacca
      481 aactcttcta tctcttcttc ttcaaaactt ccgccatgtg atatataatt caacaggtca
      541 tagcgagtga taccttcacg agctaaaaag tatgaagcaa atgattcttc ttcttcataa
      601 atggcaatga ctatatcacc aaaatccaga atctcttttt gtgcggaatt gatgtgcata
      661 atggcacgtt caataacatg ctgcaatcca acagattgtg aaggttctcc atctgcaatt
      721 acaggaatat gcattgaaag atgcttgctt aaatttcgct taagctgctc aacattgcca
      781 ccgcatcctt ctataacctc cctgccttca ggaaaataca gcgaggccaa taaaacatgt
      841 tcaggcgtaa gatattcatg cttacgattc tttgcctcat tataggcagc catcaatatc
      901 tggtttaata cggtgcttat ctccattgac aaaacccttt attacaattt attacaatta
      961 aaataccagt atacctgtca tgcctcttca acagtacact tcagcggaaa ttcacgctga
     1021 cgtgccagtg tatgtacctg tcctgccttg gtctgtgcta tatcataggt atacacacca
     1081 caaataccaa aaccatttct gtgaacctgc aacataagtt ccgttgcttc catattattt
     1141 ttatgaaata ttgtaaccag aacctctaca acaaaatcca tggttgtata atggtcatta
     1201 tgaagaataa ccttatacat gcgtggtttc tttacctgta catcctcttt aatctggaga
     1261 ttgccgtcat attcataccg atgtgccatg gatatattcc tggtttacca tgttctatta
     1321 ttattgccac cattactcta ttgtagtttg tactatacta tttattacaa tataaaaaaa
     1381 atttttattt atggcaacct ctctatccaa ttagtttttc atattcaggg acgatatcat
     1441 cggtaaattt cttcatgcct tcaaatgttt tttcatgccg taacatttga gctatcacac
     1501 caaatggtac agttgctata tgagcgcccg caagcgctgc ttcacgtacc tgccgaggat
     1561 tacgcagcga tgctgcaata atttcagatt tgtattcata atatgttact atttcaacac
     1621 actgcgatac tacatctatc cctgatacta tcccattatc atgtaacagt acaccattct
     1681 gctcaatccc ttcctcagga aaataatcgc ttttatcaaa tttgatatgt gcactgcttc
     1741 ggatataatc atcaatgcgt cctgcaaatg ggcttatata ggctgcacct gctttagccg
     1801 ctaaaagcgc ctgctcaggg gtaaacacca gcgtacagtt taccgggatg ccttcagatt
     1861 gcaaaacctt tacagcattg atgccatcaa aatgacattg ctcatccggt tgcatggcag
     1921 gattaaccgg tatctttata tt
//
LOCUS       DAOSOX010000323         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_59017_length_1940_cov_6.938992, whole genome shotgun sequence.
ACCESSION   DAOSOX010000323 DAOSOX010000000
VERSION     DAOSOX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59017_length_1940_cov_6.938992"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLX22_12935"
     CDS             complement(<1..1024)
                     /locus_tag="PLX22_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20846.1"
                     /translation="MDNSTVTLNLAYYTKKLHDLTKKSWYYCAKSVYKNRRLLAGNNP
                     VKTIFKSKSILIAIYNKCKKENFTHSQFTPLDFAFIQIFIDTYSRDVLEKVFLHSIAV
                     ELSQGQYAICTSTFCDDNFTLDDIVHFNKTASITILCHSNETAKSWPKNNICLEWYFR
                     LLEPLSNYTAFTRWLAKLFYNNTIYQNAQGIITSYDTLVNTFATYNRTTAADVIPLLS
                     KNNFWKFPDSDELYELVFDCEVAVKSTDIQQVGALLCCRGLVISTIDSLSQLKKLDAI
                     CMLRVIGYNILDFDLDILSHNNFSFSNPYEIVDLLWVYNILYPINDGTSLDDAFRRLV
                     GMSIEKR"
BASE COUNT          613 a          363 c          321 g          643 t
ORIGIN      
        1 gacgcttttc aatggacatc ccaaccaaac gcctgaacgc atcatccagc gatgtgccat
       61 cattgattgg ataaaggatg ttgtacaccc ataatagatc aactatctca tagggatttg
      121 aaaaagaaaa attattgtgc gatagtatgt ctaaatcaaa atccagaatg ttataaccaa
      181 tgaccctgag catgcatata gcatccagct tttttaactg gctcagcgaa tcaatagtac
      241 tgattaccag acccctgcag cacagcaaag caccaacctg ctggatatca gtgcttttaa
      301 ctgcaacctc acaatcaaac acaagctcat ataattcatc gctatcagga aatttccaga
      361 aattattttt tgacaaaagt ggtataacat ctgcagcagt agttctgtta taggtagcaa
      421 aggtgtttac caatgtatca tagcttgtaa ttatgccctg cgcattttgg taaatggtgt
      481 tattgtaaaa aagtttagca agccaccggg taaatgctgt ataattgctt aacggctcca
      541 gtagtctaaa ataccactca aggcatatat tgttttttgg ccaacttttt gctgtttcat
      601 tgctatgaca tagtattgta atgctggctg ttttgttaaa atgtacaatg tcatccagtg
      661 taaaattatc atcacaaaaa gtggatgtac atattgcata ctgtccctgt gatagttcaa
      721 cagctatgga atgtaaaaaa accttttcaa gaacatcacg cgaatatgta tcaataaaaa
      781 tttgtataaa agcaaaatca agtggagtaa attgcgaatg ggtaaagttt tcctttttac
      841 atttattgta tatagcaata agtatggatt ttgatttgaa tatggtttta acagggttat
      901 tgcccgccaa taatcttcgg tttttataaa cactctttgc acagtaatac cacgattttt
      961 tagttaaatc atgcagtttt ttagtatagt atgccagatt tagtgttact gtgctgttat
     1021 ccatggctct gaccccatta tattttttat ctgctacttt gtcaaggcat atgctttgac
     1081 tttacctgta tacctctacc ctcgcttttt tactgcacaa ttatgttaga ctattgtaaa
     1141 taaaaattat agatcttttt tgtatataac atattttttg gggtcagagc cttgatagag
     1201 tgatagcaac tgaagaattt attgttttta gcagggagag attattcgga tttgcctcag
     1261 aatgagggtg ctaacctttt tcaaagagga ataaaattct tcactatgtt caagttgcca
     1321 taattaacaa ttttattcaa aaaaaaatct ccatattttt tctcatgctt tattttatat
     1381 tgatgggaaa caaaagtttt atcacttcgt aatgggatat ttatgcgctc aatacccccg
     1441 tgccaacact gtggagccac ctcaaacggc tcgacttatc gttaaatttt gtggatgaaa
     1501 aaattaacaa accctcaaaa aatttcatgc gatattatcc agtttgccat ttacaaactc
     1561 ctcaccattc gcaacttgac aaatcttata gcctcgaagt ttcctccatc ctttctgtgc
     1621 ttccagagcg agtttgaatt ccatggtcaa agtcaaaatc cgcaaaccgc agcctcgcgt
     1681 ctgggaagcg ttgctctggc accttcgtct attgaaggcg gtcttaataa ttcaacgaat
     1741 gaagttacat actttgctga taaaaggata aagagaactc aaagttcact atatggattt
     1801 attaaaaatt gcattttgtt cttgactaac gttccataat aaacataaca tgatacacaa
     1861 atttcattat tatggagttt tcagaaaaac tacactatta ccaaagacaa tccttatctt
     1921 gcatattctt cagcaggctt
//
LOCUS       DAOSOX010000324         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_59859_length_1921_cov_9.546624, whole genome shotgun sequence.
ACCESSION   DAOSOX010000324 DAOSOX010000000
VERSION     DAOSOX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59859_length_1921_cov_9.546624"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLX22_12940"
     CDS             complement(<1..482)
                     /locus_tag="PLX22_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOT20847.1"
                     /translation="MNSTFMGIEIGKRGLLAHQQALHVTGHNISNAANEEYSRQRVVI
                     TASDPLYVPALNRAQQPGTIGQGAEVQSIERIRDEFIDDRIMVEKQAFGYWKTRNDFI
                     YQVEMVYNEPSDQSIRSRLDALWEGFQELSKYPEERSTREVVKEKAIALANEVKYVYG
                     "
     gene            complement(491..985)
                     /locus_tag="PLX22_12945"
     CDS             complement(491..985)
                     /locus_tag="PLX22_12945"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOT20848.1"
                     /translation="MDALHHIEPIIKKEIAIYNQLLAIEEQKKDAIIAFNGEQLFLFS
                     KEQEKYVHELENLEAARQKVLNNATKQGLQQGNISLKDFIMIASANNTENVILLGYEL
                     KKKIEKFSRLYEGNKKLIDDNMEFFNIILSSLRKATSYETGYGPEGLAIRSASGAVLM
                     NKTV"
     gene            complement(1064..>1921)
                     /locus_tag="PLX22_12950"
     CDS             complement(1064..>1921)
                     /locus_tag="PLX22_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20849.1"
                     /translation="KEKDLDEMYVLYPMQFQIISNVRSEERVDVSSAGKSVLFFTNAI
                     SDYILQNSLALSLKKVDNVKEILRYDLDKRFPYVKIYFLNEGLSDPRMKYFYNSPVPF
                     FVPDIKAQPDAKSEKIFNIFINEIYAKDYMLQNRKNLISEISVPILYHGKIPYGYIQI
                     NSEKPLTDGIFSVIRRMGVVTDELFKKYKIFIPENDRLLVADASASGIGIVFRERKYI
                     RYFKEGSYVIGDILLPNGTKANVLTVVRNITLMENKIIKVGCQIKDIDALSEVHYHEY
                     LESTGMKVE"
BASE COUNT          551 a          414 c          290 g          666 t
ORIGIN      
        1 ttaccatata catattttac ttcatttgcc agtgcaattg ctttttcctt tactacttcc
       61 cgtgttgaac gctcttcagg atattttgat aattcttgaa aaccttccca cagtgcatcc
      121 agcctgctcc gtatggactg atcggatggc tcattgtaca ccatctcaac ctgatagata
      181 aaatcattac gtgtcttcca atacccaaat gcctgctttt ctaccatgat gcggtcatca
      241 ataaattcat cgcgaatacg ctctatactt tgcacctcag caccctgacc aatagtaccg
      301 ggctgctgtg ctctattcag agcaggaaca tataatggat ctgaagcagt tatgacaaca
      361 cgctgacggg agtattcttc attggcagca ttggatatgt tatgccctgt tacgtgcagc
      421 gcctgctgat gagcaagtaa tcctcgtttc ccaatctcta ttcccatgaa tgttgaattc
      481 atattatacc tcacaccgtt ttattcatta aaactgctcc actagcactg cgtatggcca
      541 atccttcagg accatatcct gtctcatagc tggttgcttt tcgcaatgat gaaaggatta
      601 tattaaaaaa ttccatatta tcatctatga gctttttgtt gccctcataa agccgtgaaa
      661 acttttctat ttttttcttt aattcatacc ccagcaatat tacattctca gtattattag
      721 cgctggctat cataataaaa tctttaagtg atatattgcc ttgttgtaat ccctgttttg
      781 ttgcattgtt taataccttc tggcgtgctg cttctaaatt ttctaattca tgtacgtatt
      841 tttcctgttc tttagaaaat aaaaaaagtt gctccccatt gaacgctatg atagcatcct
      901 ttttttgttc ttctattgcc aataactgat tatatattgc tatctctttt ttgatgatag
      961 gttctatgtg gtgcagggca tccatatatt ccctccttgg aaaaatgcta atactactat
     1021 tacctatcgg aattttacaa aatagattga ggatttttat atgttattcc actttcatgc
     1081 cagtagattc cagatattca tgataatgca cctcactcaa ggcatcaata tcctttatct
     1141 gacaaccaac tttaatgatc ttattttcca tcaatgtgat gttccgtaca actgttaata
     1201 cattggcttt agtcccattg ggtaacagta tatcgccaat cacatagctg ccttccttaa
     1261 aatagcgaat atattttctt tccctgaaaa caatgccaat accgctggct gacgcatcgg
     1321 caacaagcag acggtcattc tcgggtataa atatcttata ttttttaaaa agctcatcag
     1381 taacaacacc catccgtcgt attaccgaaa aaataccatc ggtcagcggc ttttcgctgt
     1441 ttatctgaat atagccatag ggaattttac catgatataa aattggcacc gatatctcag
     1501 agattaaatt cttcctgttt tgcagcatat aatcttttgc ataaatctcg tttataaaaa
     1561 tattgaatat tttttcactc tttgcatcag gctgcgcttt tatatcagga acaaaaaagg
     1621 gtaccggact attataaaaa tatttcatgc gtgggtctga caatccttca ttgagaaaat
     1681 atatttttac atacgggaaa cgtttatcaa gatcataccg taaaatctct tttacattat
     1741 ctactttttt aagagaaaga gccagtgaat tttgcaatat gtaatctgaa atagcgttgg
     1801 taaaaaacaa aaccgacttc cccgcagacg atacgtcaac cctttcttca ctgcgcacat
     1861 ttgaaattat ctgaaactgc atcggatata aaacatacat ttcatccaga tctttttctt
     1921 t
//
LOCUS       DAOSOX010000325         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_60143_length_1914_cov_5.100592, whole genome shotgun sequence.
ACCESSION   DAOSOX010000325 DAOSOX010000000
VERSION     DAOSOX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60143_length_1914_cov_5.100592"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLX22_12955"
     CDS             complement(<1..627)
                     /locus_tag="PLX22_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT20850.1"
                     /translation="MCGRFAQIEPVDSIAQRLGIDEVLMDTIAPRYNISPGENVVALV
                     HTMKKSLVQLRWGLIPYWVRDISRVKPLINARVETVAVKPSFKKAFFNNRCIILACGF
                     YEWKKEGSKKIPYYITIDDFFGFGGIFDTAIVGGKELRTCAILTTEANDAMRVIHNRM
                     PLIIRQKEFELWIKSETPVDILKELIKPYRGNCIMHPVTAKVNNPAYDE"
     gene            complement(629..>1914)
                     /locus_tag="PLX22_12960"
     CDS             complement(629..>1914)
                     /locus_tag="PLX22_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOT20851.1"
                     /translation="ISNQDSSKTDTKPFEQFHTFLAKTYPELHKTLKKEVVKGYALLY
                     TWKGSDPSLKPILLMAHQDVVPVDAVTLDQWTHPGFSGAIADGYIWGRGALDVKNALI
                     SIMESIEYLVNKGFVPKRTIYVAFGHDEEVGGEGAKAIAKLLKDRNVQLEYVLDEGGA
                     IMEGALPGISNPIAMVGIAEKGYLTLKLTVKGTGGHSSMPPHHTALGVLARALVAIED
                     NPFPREIKGPAQQMFEYVTPHMSFPLKVMFANMWCFKPLLKWQFEKTESSNAIMRTTV
                     ALTMAGASEKDNILPTQAWAMINCRILPGETMASTTAYIKKIIDNEEVLIASTPWSND
                     PSPVSDINAPSFRSIATITRQIDNSIIVAPYLVLGATDSRNYNALTNQIYRFTPVKMN
                     NEDLKRTHGINERISISDMGKSVKFFIALIEKTHQ"
BASE COUNT          541 a          422 c          343 g          608 t
ORIGIN      
        1 ctcatcatac gcagggttat tgacctttgc tgtaaccgga tgcatgatac aattacccct
       61 atagggtttt atcaattctt tcaatatgtc cactggtgtt tcagatttta tccagagctc
      121 aaattctttt tgccgtataa taagaggcat cctgttatgg attacacgca ttgcatcatt
      181 tgcttcagtg gtaagtatag cacatgtacg caactccttg ccaccaacga tagcagtatc
      241 aaatatccct ccaaaaccaa aaaaatcatc gatggttata tagtagggta tttttttact
      301 gccttccttt ttccactcat agaagccgca tgccagtata atgcacctgt tattgaaaaa
      361 tgccttttta aaacttggct tcacagcaac tgtttcaaca cgtgcattaa tgagtggttt
      421 tactcttgat atatccctta cccaataggg gataagcccc catcgcaatt gcactaatga
      481 ttttttcata gtatgcacca gcgctacaac attctcgcct ggacttatgt tgtagcgcgg
      541 tgcgatagta tccattaaaa cttcatcaat tcctaaacgc tgtgcaatac tgtcaacagg
      601 ctcaatttgt gcaaatcgcc cgcacatatc attgatgcgt tttttcaatc agtgcaataa
      661 agaatttaac gcttttaccc atatcagaaa tacttatacg ctcattgatg ccatgggtgc
      721 gttttaaatc ctcattattc atctttacgg gtgtaaaccg gtaaatctga tttgttaaag
      781 cattataatt tcttgaatca gttgccccca atactaaata gggagctaca atgatagaat
      841 tatctatctg tcgggtgata gttgctatgg aacgaaatga aggtgcattg atatccgaaa
      901 ccggtgaagg atcatttgac catggtgttg atgcaatgag tacttcttca ttatcgataa
      961 tcttctttat atatgcagtg gtactggcca ttgtttcacc gggcaggatt cggcaattta
     1021 tcattgccca tgcctgtgtt ggcaatatat tatccttttc gcttgcacct gccatggtaa
     1081 gtgcaacagt agtacgcatt attgcattag aggattcagt tttttcaaac tgccacttta
     1141 ataatggctt aaaacaccac atatttgcaa acataacctt taaaggaaaa ctcatatggg
     1201 gagtaacata ctcaaacatc tgctgtgctg gacctttaat ttctcttggg aatggattgt
     1261 cttcaatagc aaccaatgct ctggcaagta cccccaatgc ggtatggtga ggtggcatgg
     1321 atgagtgccc acctgtacct tttaccgtca acttcaatgt taaatatcct ttttcggcaa
     1381 tccctaccat ggctatagga tttgatatcc ccggtagcgc cccctccatg atagcaccgc
     1441 cttcatccag aacatattcc agttgaacat tacgatcttt taaaagttta gcaatagctt
     1501 tagcaccctc accaccaacc tcttcatcat ggccaaatgc tacatagatt gtccgtttgg
     1561 gaacaaaacc tttatttacc agatattcta tagactccat gatggaaatt aacgcatttt
     1621 taacatcaag tgcacctctt ccccagatat agccatctgc tattgctcct gaaaaacccg
     1681 gatgagtcca ctgatcaagc gtaacagcat ctacagggac aacatcttga tgagccatta
     1741 aaagtattgg cttcaatgag gggtcagagc ccttccatgt gtagagcagt gcataccctt
     1801 taacaacctc tttttttaat gttttatgca actcaggata tgttttggct aaaaatgtat
     1861 gaaactgctc aaatggttta gtatcagttt ttgagctatc ctgattggaa attg
//
LOCUS       DAOSOX010000326         1907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_60455_length_1907_cov_10.164147, whole genome shotgun
            sequence.
ACCESSION   DAOSOX010000326 DAOSOX010000000
VERSION     DAOSOX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60455_length_1907_cov_10.164147"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLX22_12965"
     CDS             complement(<1..550)
                     /locus_tag="PLX22_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT20852.1"
                     /translation="MLTNNAGSYHERIEYLPYGETWVEDKVTSDSYTTPYKFTSKELD
                     AETGLYYFGARYYDARVSRWVSADPALAEGKYFPKPNDYDTEHDFYWYLQQDGSKKLA
                     GLGGVYNAVNMDVYHYAGDNPVKLVDPDGKTTIIIGIKAEVAGSVGSACYTGIAMDFS
                     ERNVYIFNNISMTAGTRADIGVD"
     gene            complement(651..1304)
                     /locus_tag="PLX22_12970"
     CDS             complement(651..1304)
                     /locus_tag="PLX22_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20853.1"
                     /translation="MSTIGEYIILIFILIILPSCLSKMTVNKVKEVEGITINVGGKEY
                     LLNIFPVGMYYVENKYKKEEIELNFKKNKFNKRIDEVGSVIYIIAFGGKDVPIFHFNL
                     IFPFRKDNKYFAIPWDSRRNNEPMYVILDFKKPYSGRRYEINEEINYNYVTVKEMGKT
                     VKGAFHAKLVGLGSRDIIEIKGRFKVKLIYDEREFQEYEDEVEKSWDDVIYPLYEIK"
     gene            complement(1349..1819)
                     /locus_tag="PLX22_12975"
     CDS             complement(1349..1819)
                     /locus_tag="PLX22_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20854.1"
                     /translation="MKHYNAWNEKTQYLHSMRGKKEIEENGKKKKVDDETIEEAQQKA
                     KEWIEKNVNEAVESAIKEDYKDALYKFGQALHTEQDSVSHGWCTIKEHGKSLKAFGMD
                     ANPSQTKQTMAQQKTKDLTNQFLNAIRTTMMNRGYTNEQITKTIEGLKQYKPAK"
BASE COUNT          527 a          409 c          226 g          745 t
ORIGIN      
        1 tatcaacacc tatatctgct cgtgttcctg ctgtcatact tatattatta aatatatata
       61 catttctttc agaaaaatcc attgcaatac ctgtatagca agcagatcca acagaccctg
      121 ctacttcagc ttttattcca attattattg tagttttccc atccgggtcc accagtttca
      181 ccggattatc cccagcatag tgatacacat ccatatttac tgcattatac actccaccta
      241 atcccgctaa ctttttgctg ccatcttgct gtaagtacca gtaaaaatca tgttctgtgt
      301 cataatcatt aggtttcgga aaatacttcc cttctgcaag cgccggatcc gcactcaccc
      361 atctgcttac ccgtgcatcg taataccgtg caccaaaata atatagtccc gtttccgcat
      421 ccaattcctt tgaggtgaat ttatatggcg ttgtatatga atctgatgtt actttatcct
      481 ccacccatgt ttcgccgtag ggcagatact ctatacgctc gtggtacgaa ccagcgttat
      541 ttgttagcac actgcttgaa ccaagatggt cactggcgta aaaatagatt tgccaggatt
      601 gctatattct catttaatac tattaattag aatcccaatg ttcattataa ttattttatt
      661 tcgtacagtg gatatattac atcatcccat gacttctcta cttcatcttc atattcttga
      721 aattctcttt catcataaat caacttaact ttaaatctac ctttaatctc gataatatca
      781 cgggaaccta atcccactaa tttagcatgg aacgcccctt taaccgtttt tcccatttct
      841 ttaactgtaa cataattata atttatttct tcatttattt cataacgccg cccactatat
      901 ggttttttaa aatcaagtat cacatacatg ggttcattat tacgcctact atcccagggt
      961 atagcaaaat acttattatc tttacgaaat gggaaaatta ggttgaaatg aaatatcggg
     1021 acatctttgc caccaaatgc aattatatat ataaccgatc caacttcatc aatcctttta
     1081 ttaaatttat tttttttaaa gttcaattct atttcttctt ttttatattt attttcgaca
     1141 taatacatcc ctaccgggaa aatatttaat aaatattctt tacccccaac atttattgtt
     1201 ataccttcca cttctttaac cttatttact gtcatttttg ataaacatga tggtaatatt
     1261 attaaaataa atatcaaaat aatatattca cctattgtac tcatcttttt catcataatc
     1321 cttaaccata aaatcctgaa attttttctt atttcgccgg cttatattgt tttaagcctt
     1381 caattgtttt agttatttgt tcatttgtat atcctctatt catcatcgtt gttctaattg
     1441 catttaaaaa ttgatttgtt aaatctttag ttttttgttg tgccattgtt tgtttcgttt
     1501 gagaaggatt agcatccatt ccaaatgcct ttaaactctt cccatgctcc tttattgtac
     1561 accaaccatg tgatactgaa tcttgttcgg tatgcaaagc ttgaccaaat ttatataatg
     1621 catctttata atcttcttta attgcagatt caacagcttc attcacattt ttttcaatcc
     1681 attcttttgc tttttgttgt gcttcttcta tggtctcatc atctaccttc tttttcttcc
     1741 cattttcttc tatttccttt ttacccctca ttgaatgtaa atattgtgtt ttttcattcc
     1801 atgcattata atgtttcaca ccaaatgaga attttcagca gtcaaagata gggagtaatt
     1861 tcgtgtgatg ctatttgctt cacgcctcaa cctgcgagct ttgctta
//
LOCUS       DAOSOX010000327         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_60815_length_1899_cov_7.727766, whole genome shotgun sequence.
ACCESSION   DAOSOX010000327 DAOSOX010000000
VERSION     DAOSOX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60815_length_1899_cov_7.727766"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLX22_12980"
     CDS             <1..751
                     /locus_tag="PLX22_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20855.1"
                     /translation="RYIMKKKVIVYYSYIVAIPIFLLSFLLSCATMNTGDTRLIDQLK
                     AENRVCSQNLNLFMRENDVLKAENAKYKKENNALRSSNTLLKSDIGALQTRYKNDIAM
                     WDERYNTLQQKYDVFVKQSDAKVQELTELNKTLEKRMADEIARLNEVIRKQEVDFIKE
                     KETIINQNAKKEMEYQSTIDALQKQVTTLTQEKEQLKSKLTEMEINCEKLKQQLDVPQ
                     KDQTLRGGDKGTANDVKKEKLAQQLKTELEK"
     gene            772..>1899
                     /locus_tag="PLX22_12985"
     CDS             772..>1899
                     /locus_tag="PLX22_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20856.1"
                     /translation="MGLRIYSAVFLVCFVYVFPSYCFAYETLTIIATSNLRGNFSLDT
                     DEKTDPLLKVARGIIAERSAKPVDCYVDLGNAFSPGALSRYSYGAAMLDFFEYVQCDA
                     TLVGSRDVAVGLKNLELASRMRPTQLLSINIVIDNKPIFKPYWILQKAGTKIGFIGIT
                     SSKSIFDVAEKKVLSASLAAYRDYLTMTIESLQAMGCKYIIVLSGLSGAENIAIMKDY
                     PAIDLILAGGDASGIYYDVYSPRVDLEDGRSIITLVRAGGHYSVRLMLKEKITIEKIY
                     FSEADQKVSDSSYNELVERVNLWKERYAEAGAAALADNFPATDISSVTIAHLLRHYYN
                     SEVAIVDVTDVFSTRLEGTITSIDVQRIVQNDFTVFTYRLSG"
BASE COUNT          624 a          305 c          425 g          545 t
ORIGIN      
        1 gagatacata atgaaaaaaa aagttatagt gtattatagt tacattgttg ctatcccaat
       61 ttttttacta tcatttttac tatcatgtgc aaccatgaat actggtgata ccaggcttat
      121 tgaccagctt aaagctgaaa acagggtttg ttcgcaaaac ctgaatcttt ttatgcgtga
      181 aaatgatgtg cttaaagctg aaaatgctaa atataaaaaa gaaaataatg ctttgcgctc
      241 ttcaaatact ctattgaaat cggatatcgg tgcattgcag accaggtata aaaatgatat
      301 tgccatgtgg gatgaacgct ataatacact gcaacaaaaa tatgatgtat ttgttaaaca
      361 gagcgatgca aaggtgcagg aattgacgga actaaataaa acgttagaaa aaaggatggc
      421 agatgagata gcgcggttaa atgaggtaat acgcaaacag gaagttgact ttattaaaga
      481 aaaagaaaca atcattaatc aaaatgcaaa gaaggagatg gagtaccaat caactattga
      541 tgctttgcag aaacaggtaa ccacattaac tcaggaaaaa gagcagctta aaagcaaatt
      601 gacagagatg gaaataaatt gtgaaaaatt aaaacagcaa cttgatgtgc cacaaaaaga
      661 tcaaacttta aggggtggcg ataaaggcac tgcaaatgat gtgaaaaaag agaagctagc
      721 ccagcagtta aagacagagc tggaaaaata aaaaggtgaa gaaaaatagg catgggtctt
      781 cggatatata gtgctgtttt tttagtttgt tttgtttatg tatttccttc ttattgcttt
      841 gcctatgaaa cgcttaccat tattgccacg agtaacctca ggggcaattt ttcactggat
      901 actgatgaaa aaacagatcc attgctaaaa gttgctcgtg ggataattgc ggaaaggtca
      961 gcaaagccgg ttgattgcta tgttgattta ggaaatgcat tctcaccagg tgcattgtca
     1021 cggtatagct atggtgcagc gatgctcgat ttttttgaat atgttcaatg cgatgcaact
     1081 ttggtgggct cacgggatgt tgcagtagga ttaaaaaatt tagaattagc ctcgcgtatg
     1141 cgacctacac agcttttatc tattaatatt gtcattgata acaagccaat atttaaaccg
     1201 tactggatat tgcaaaaagc tggcacaaag ataggattta taggtatcac atcttccaaa
     1261 agtatatttg atgtggcaga gaaaaaggta ttgagtgcat cgcttgccgc ttatagggac
     1321 tatcttacca tgaccattga atcgttgcag gcaatggggt gcaagtatat catagtgctt
     1381 tcaggtttat ccggtgccga gaacatcgct atcatgaagg attatcccgc aattgacctt
     1441 atccttgctg gtggtgatgc ttcagggatt tattatgatg tgtattcccc acgggttgat
     1501 cttgaagatg gacggtccat cattacgctt gtcagggcag gtggacatta tagcgtgcgt
     1561 ttaatgctga aagagaagat taccattgaa aagatttatt tttcggaagc agatcaaaag
     1621 gtaagcgata gttcatacaa tgagcttgtt gaacgtgtca atttatggaa agagcggtat
     1681 gcagaggcag gagctgctgc tcttgctgat aactttccgg caaccgatat atcttcggtg
     1741 actatcgcac acctgttacg tcactactat aacagtgagg ttgccattgt tgatgtaact
     1801 gatgtattct caacacgatt ggaagggacg attaccagca ttgatgtaca gcgaattgtt
     1861 cagaatgact ttaccgtttt tacttaccgg ttaagcggg
//
LOCUS       DAOSOX010000328         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_60816_length_1899_cov_5.477766, whole genome shotgun sequence.
ACCESSION   DAOSOX010000328 DAOSOX010000000
VERSION     DAOSOX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60816_length_1899_cov_5.477766"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(438..803)
                     /locus_tag="PLX22_12990"
     CDS             complement(438..803)
                     /locus_tag="PLX22_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20857.1"
                     /translation="MKLKGLRNETVRGDSVYRSEEIEEMVRKKGFTSKIHEKGYRGKP
                     LTERQKARNTIQSRIRARVEHIVGSIHWLKGDYMLCIEIERITEAVGIVNLAYTMKRY
                     CLYGCCGCPVFFIFFRKIF"
     gene            complement(1489..>1899)
                     /locus_tag="PLX22_12995"
     CDS             complement(1489..>1899)
                     /locus_tag="PLX22_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20858.1"
                     /translation="GAFMIIERMGLKKILDKYGFIGTIYSILVVMIGWVFFRSPDIQF
                     ALKYIYLMFSFNDYQAVHFDAMRYVTNEFWIYVVIATLFSFPIVPYLKKRMNAYQELL
                     WYKLSVHGYTIILFIIAFMYIAKSSYNPFIYAGF"
BASE COUNT          614 a          393 c          266 g          626 t
ORIGIN      
        1 aatactataa actgccatcg ggatacacaa ctcggggcat aaccaaacca acatctttat
       61 tcttatccat gaaggtataa agctcttcca atacaccatt tttgaaatac acatcgggat
      121 ttagcactac atggtatggg ataccatctt caatactttt ttgtagtgct atattatgag
      181 ctttaccata gccaaggtta gcatcattaa aatatattct atccggttat catctatatg
      241 cactatatca tctaaattac tatttattga attatcaatc agatacagct taacttttaa
      301 atctgtattt aataaactat ggatactttt cttcaggtag tccctggggc tattatataa
      361 aactattgat gcattaatgt gctgcattta tttcctcaac atgtgttaaa cttttaccta
      421 tttttatgaa tcttttttca aaatattttt ctgaaaaata tgaaaaacac agggcatccg
      481 caacatccgt agagacaata ccgcttcatg gtatacgcca gatttacaat gccaaccgct
      541 tcggtaatcc gttcaatttc aatgcatagc atataatccc cttttagcca gtgaatgctt
      601 ccaacgatat gttctacgcg tgccctgatt cttgattgaa tcgtattgcg cgctttctgg
      661 cgttctgtaa gtggtttgcc cctgtatcct ttctcgtgaa tcttgctggt aaatcctttc
      721 ttccttacca tttcctcaat ctcttcagag cggtagacgc tatctccccg aaccgtttca
      781 ttcctcaagc ctttcaattt catcacataa tccacactgt ttcataatcc tcaacttttt
      841 gtgattgatg tatggagatt gtgtccgcat agtctcccct gtccattaat gtatgacaaa
      901 aatctttaat ttttagaggt tgcctataat caaaagctca aacagggaca aaacaatcac
      961 tgcggtaaaa acctattcca gcatttgtca ctctcaggtt atgcagcact tttcatcacc
     1021 tttaggggcg ccgttctttt cgtcattctg agaacataca gcccattctg tcattctaaa
     1081 ggggcgaagt cctgaagaat ctactttttc atttaaaggc gaaatctttc gcctgcggct
     1141 taaggatgac atgtcatcac attgtcattc cgggcttgac ccgggatctt aacttcgcat
     1201 accagtcatg ctgaacttga ttcagcatct cattattgat atagatcctg aaaccagttc
     1261 atgataacta tcgccaaaaa aacattatgt tgcttttttg agcgcatcac aatgcatccc
     1321 tataaattta attatcccac aaattacctg cttgagcaat atattccttc aaaaggtata
     1381 aagaataatt aaccttttaa tgcaatatac aatactatat ataaaataaa tcatttatgc
     1441 agtaatagtg tcaacaaaaa atataagttg ctatttaact gtaacgaatt aaaatccagc
     1501 atatataaat ggattatatg aactttttgc aatatacata aacgctatta taaacaaaat
     1561 tatagtatag ccatgaacag acaatttata ccatagcaat tcctgataag cattcatacg
     1621 cttttttaag tatggcacaa tggggaatga aaatagagta gctataacta cataaatcca
     1681 gaactcattg gtaacatacc gcattgcatc aaaatggact gcctggtaat cattaaagga
     1741 aaacattaaa taaatatatt ttaatgcaaa ctgtatatca ggtgacctga aaaaaaccca
     1801 tccaatcata acaactaata tagaataaat agttccgata aaaccatatt tatcaagtat
     1861 ttttttcaat cccatccttt ctataatcat aaatgctcc
//
LOCUS       DAOSOX010000329         1892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_61159_length_1892_cov_9.934676, whole genome shotgun sequence.
ACCESSION   DAOSOX010000329 DAOSOX010000000
VERSION     DAOSOX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61159_length_1892_cov_9.934676"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /gene="murJ"
                     /locus_tag="PLX22_13000"
     CDS             <1..1389
                     /gene="murJ"
                     /locus_tag="PLX22_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOT20859.1"
                     /translation="EGVLSASFIPVYAKLIAQNKHDEAAKVAHAIAGILSLLISTLVL
                     VGIIITPLLITVIAPGFEGQKRELTITLVRILFPGAGVLVFSAWCLGILNSHGKFFLS
                     YSAPVLWNVVIIITLILFGDSSSQDELAKYAAAGSVIGSLLQLLMQLPVVIGILHHIK
                     LSFTVKTKEVKTILKNFVPVFISRGVVQISAYIDALLASLLPTGALAGLSYAQTLYML
                     PISLFGMSVSAAELPAMSKVSGNTRDVAKGLQKRINNSIKRIAFYIVPSTAGFFILGD
                     CIVAAIYQTGKFTTRDTFFVWMVLAGSTVGLFATTQSRLFSSAFYAMHDTKTPLKCAL
                     IRVALSSTLGYCASQYAPYLLHIDKLYGTVGITVASGLSGWVEYRMLRYTLSKVVGKV
                     ALGKRYIVSLWVSALIAAGVAYLIKSVLHYHPITIAFIVLSLYGMIYFALSLLFKIDE
                     MKNLLSMIGIKK"
     gene            complement(1396..>1892)
                     /locus_tag="PLX22_13005"
     CDS             complement(1396..>1892)
                     /locus_tag="PLX22_13005"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOT20860.1"
                     /translation="YLDGLRTIDALRLHNDLDCSRIAIVGKGLGAAVSVFTASHSNRV
                     VAMVLDTPSFCYIDMAQNIAKSTIANEINEFIEEHKNKKSVIKKTLAYFDALQFTNKI
                     SCPVLATVGFKDTLSPAECVFALFNHLQCDKTIEVYPEEGHIPGGIEQMNRMIEWIKN
                     QFEK"
BASE COUNT          523 a          362 c          389 g          618 t
ORIGIN      
        1 gagggtgttc tttctgcttc gtttatacca gtatatgcaa agttgattgc acaaaacaaa
       61 catgacgaag cagcaaaggt agcacatgca attgcaggca ttctttcgtt attaatttcc
      121 acccttgtac tggttggtat tattatcact cctttgctta tcaccgtcat tgcaccaggc
      181 tttgaagggc aaaagcgtga gcttaccatt actctggtac ggattctttt tccgggagcg
      241 ggggttcttg ttttttcagc gtggtgtctg ggaattttaa atagtcatgg aaaatttttt
      301 ttatcgtaca gtgccccggt attatggaat gttgttatca tcattacctt aattttgttt
      361 ggcgatagct cctcacagga tgagcttgca aaatatgctg ctgcaggctc ggtcattggc
      421 agtttactgc agttgttgat gcaactgccg gttgtgatag ggatactcca tcatataaaa
      481 ctatcgttta cagttaagac taaagaagta aaaactatat taaaaaattt tgtaccggta
      541 tttataagca gaggagttgt tcagataagc gcttacattg atgcattgct tgcaagcctt
      601 ttgccaactg gtgctcttgc agggttgtcg tatgcgcaga cgctgtatat gctcccaatc
      661 agtttatttg gtatgtcagt atcggcagca gagctccctg ccatgtcaaa ggtaagcggc
      721 aatacccgcg atgtagcaaa aggattacag aagcgaatta acaactcaat caaacgcatt
      781 gcattttata tagttccgtc aactgctgga ttttttatac tgggtgattg catagtagct
      841 gctatatatc aaaccggtaa atttacaaca cgcgatacct tttttgtatg gatggtattg
      901 gcaggttcaa ccgttggttt atttgcaaca acacagagca ggttgttctc atctgcattt
      961 tatgccatgc atgatacaaa aacaccatta aaatgtgcac ttattcgtgt tgcgctatca
     1021 agtacattgg gttattgtgc ctcgcaatat gctccatatc tattgcatat cgataagctc
     1081 tatggtacgg taggcattac ggttgcttca gggttatccg ggtgggttga atacaggatg
     1141 ctgcggtaca ccttatcgaa agttgttggg aaagtcgcac ttgggaaacg atatatagtg
     1201 tcgctatggg taagtgctct catcgcagcg ggagttgcct atctcatcaa aagtgtgctt
     1261 cactatcatc caataactat cgccttcatt gtattgtcgt tgtatggtat gatatatttt
     1321 gcgttgtcat tgctctttaa aatagatgag atgaaaaatc tattatcaat gattggaatt
     1381 aaaaaataga atatcttatt tctcaaactg attttttatc cattcaatca ttcggttcat
     1441 ctgttctatg ccacctggaa tgtgcccttc ttcgggatat acctcaattg ttttgtcaca
     1501 ttgcaaatga ttaaaaagag caaaaacaca ctcagcaggt gaaagcgtat ctttaaaacc
     1561 cactgttgct aatactggac atgaaatttt attggtaaat tgtaaagcat caaagtaggc
     1621 taacgttttt tttataacag attttttatt tttatgttcc tcaataaact cattgatctc
     1681 gtttgctatg gtactttttg caatgttctg agccatgtca atataacaaa acgagggtgt
     1741 atcgagcacc attgcaacaa ccctgtttga atgtgaagcc gtaaaaacgg aaactgccgc
     1801 gccaagcccc ttgccaacaa ttgctatcct gctgcagtca agatcattat gtaatcgtaa
     1861 ggcatcaata gtccttaagc cgtccagata ta
//
LOCUS       DAOSOX010000330         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_61667_length_1881_cov_5.893209, whole genome shotgun sequence.
ACCESSION   DAOSOX010000330 DAOSOX010000000
VERSION     DAOSOX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61667_length_1881_cov_5.893209"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLX22_13010"
     CDS             <1..339
                     /locus_tag="PLX22_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT20861.1"
                     /translation="PWAMIPDTVEYSQWKTGIRREGILYGFFYFSQKLAAACAGFIIG
                     IGLTLCGYLQPFLVDNTLTSRMQTEGALTGIRLLSTIIPMLLIIVGIVFIWLFPIDEK
                     MHRKIVEEIG"
     gene            414..683
                     /locus_tag="PLX22_13015"
     CDS             414..683
                     /locus_tag="PLX22_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20862.1"
                     /translation="MIYYDKEVQKAIKKSDIPTKIVKRFHNAFVSLDLTKDLNLFDVK
                     KVKGSFRRNYCRLRKGKFRTMFFIENDDFYIVYIGKREEVYDQWQ"
     gene            674..883
                     /locus_tag="PLX22_13020"
     CDS             674..883
                     /locus_tag="PLX22_13020"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HOT20863.1"
                     /translation="MAVTSVRFNAKEEKIVKILKKHYNCDASTLIKKSLWELYEDIKD
                     KIIEAFEKCEKGGKTKFVTIDKMVQ"
     gene            1245..>1881
                     /locus_tag="PLX22_13025"
     CDS             1245..>1881
                     /locus_tag="PLX22_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT20864.1"
                     /translation="MNLKKLIRNYLSFVNFSTMIQLTMHWLWFRRKRVFFIFEGREYT
                     YNEVYKQSKRYATFFLSMRKKLIESGRLHDNQRLAFGVYMDNNPEFIFASFGAGLSNS
                     ILFAINTGFRGETLAKVIDQANISFLIINESTVHEVAPIINNSTVIHADDIYYAGNNN
                     GYAAQGFHSLEEAVDHAQHENIPKNYRTKIDNFSPVIVIYTSGTSGLPKGVP"
BASE COUNT          628 a          298 c          370 g          585 t
ORIGIN      
        1 ccatgggcaa tgatccccga tactgttgaa tactcgcagt ggaaaacagg catacgcaga
       61 gaaggcattt tgtatggatt tttttatttc agccagaaat tagcagcagc ctgtgcggga
      121 tttatcatcg gtattgggct aacgctgtgt ggctacttgc aaccgtttct tgttgataac
      181 acattaacct cccgaatgca gactgaaggt gcgcttaccg gtatacggct gctgagtacc
      241 attatcccca tgctgcttat tatcgtcggt attgtgttta tatggctttt ccccattgat
      301 gagaaaatgc accggaagat agtggaagaa attgggtaag gtggttatgt aaaaattcat
      361 taaaaatcat cagaacaatt aacaaattgc aattaatgta gtacattgta aacatgattt
      421 attatgataa ggaagttcaa aaagcaatta agaagagtga tattccgaca aagatagtaa
      481 aacgatttca taatgccttt gtatcgcttg atttaacaaa agatttgaat ttgtttgatg
      541 tcaaaaaggt gaaaggaagt tttagaagaa attattgtcg attacggaaa ggaaaattca
      601 ggacaatgtt ctttatagaa aatgatgact tttatattgt ttatatagga aagcgagagg
      661 aggtatatga tcaatggcag taacaagtgt acgatttaat gcaaaagagg aaaagatagt
      721 aaagattctt aaaaaacatt ataactgtga cgcttccacg ctcataaaaa aatccctgtg
      781 ggaattatat gaggatataa aagataagat aatagaagcc tttgaaaagt gtgaaaaggg
      841 tggaaagaca aagtttgtaa ccattgataa aatggtacaa taattataat tgtttataat
      901 tgaaagaaaa gcctcttgtg tacagtagta aagcctggtt gctatatcat aatgcttatg
      961 tttcctgcat agtatgccac caaacattac ctattaaaaa tacattaaaa cttctttaaa
     1021 aagtttacat atcgttgtca tgatgtaaca ttgtcatata tattattttt ttgttgcgca
     1081 aatattctat tgttatgaac ttacaaatag gtatatcatt tttttctata atgtatttat
     1141 tatacaattt tgtattatta tgaaaaggat agcatgactg atggattatg tatacatacc
     1201 ggttaggcta acgatatatc ttttataaca ggagggtatt atatatgaat cttaaaaaac
     1261 tgatacgcaa ttatttgagc tttgtcaatt tcagcacaat gattcaatta acaatgcact
     1321 ggctctggtt caggcgaaaa cgggtctttt ttatctttga aggtcgtgaa tacacgtata
     1381 acgaggtgta taaacagtca aagcgctatg caacattttt tttaagcatg cgtaaaaagc
     1441 ttattgaaag tggaaggttg catgacaatc aaaggcttgc ttttggcgtt tacatggata
     1501 acaacccgga atttatcttt gcctcgtttg gtgcgggttt gagcaactct atcctctttg
     1561 ctatcaatac cggattcaga ggggaaacac tggcaaaggt tatagatcag gcaaacatct
     1621 cgtttctcat tatcaatgaa agcacagtac atgaggttgc accaatcatc aacaatagta
     1681 cggtgattca tgcagatgac atctattatg ctggcaacaa caatggttac gctgctcagg
     1741 ggttccactc ccttgaggaa gctgttgacc atgctcagca tgaaaatatc cccaaaaact
     1801 atcgaacaaa aattgataat tttagtcccg tcattgtcat ctatacctcc ggcacatcag
     1861 ggctgcccaa aggcgttccc t
//
LOCUS       DAOSOX010000331         1877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_61861_length_1877_cov_6.589462, whole genome shotgun sequence.
ACCESSION   DAOSOX010000331 DAOSOX010000000
VERSION     DAOSOX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61861_length_1877_cov_6.589462"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PLX22_13030"
     CDS             <1..1080
                     /locus_tag="PLX22_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20865.1"
                     /translation="QHSKTFFSYKGQYQWEVPALILPEGLPYIPQEFFADTSIHDPLW
                     EDVYHWLVKKQDEVMLINNNHETTKQKALLHDDSYAIVTDDGVEHNNEFNYRNYLSAT
                     HIDDDKLMFKDEQKFLPSGVHYFDEESTTKRTIKNKVIAHAKKDEWCTTERSYMDIIS
                     CIEKINLSDSSHYSQHIKSIIDYMSHSNEWYYTHSRFNRRYVAKGIYNAGRAYKQNEI
                     ITVAIDISASMMSQKDDIETAFGIVDDLTGKYKVYLVCVDEEYFIPVKDGDALIRANR
                     LTKPYEYKKGDWKLIKSGYGGATFFSSFFNEYLQHHSEMVLVITDGYIYDIDKLKPYP
                     ATLWLVAKSRNEAFVPPFGKAIPMV"
     gene            1090..1800
                     /locus_tag="PLX22_13035"
     CDS             1090..1800
                     /locus_tag="PLX22_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20866.1"
                     /translation="MDEHINTNYNRAQSLYREGIENTAHSLQYFDDLLNCNISEFIIK
                     AGRDWKNFDVDAAMDFFISNNDIEAFYHAGIYWKNFNYERGITVIVEWGNDEYIFRAG
                     RFWKQFDYNRGLSKLIELQSAKYIYHAGLDWKQFDFEKGFDALMLIGDPEYIFYAGTH
                     WTVFNYMVATDKLIFIGNCEYIYKAGYQWKWFDYYKGWKIMESKIVEGRLWRGKALQT
                     DVWKNALKTIWGKAIANK"
BASE COUNT          655 a          279 c          389 g          554 t
ORIGIN      
        1 cagcacagta aaaccttttt ttcatataaa gggcaatacc agtgggaggt gccggcatta
       61 atactaccgg aggggttacc ctatatccct caagaatttt ttgccgacac cagcatacat
      121 gacccgctgt gggaggatgt ttatcactgg cttgttaaaa aacaggatga agtaatgctg
      181 attaataata atcatgaaac tacaaagcaa aaagctttac ttcatgatga tagctatgcg
      241 atagttactg atgatggagt tgagcataac aatgaattta attatagaaa ttatttatcc
      301 gccactcaca tcgatgatga taaattgatg ttcaaggatg aacagaagtt tttgccatca
      361 ggtgtgcatt attttgatga agaaagtaca accaaacgga ctattaaaaa taaggttatt
      421 gcacatgcaa aaaaggatga gtggtgcacc actgaacgct catatatgga catcatatca
      481 tgtatagaaa aaataaatct tagcgatagt tctcattaca gccagcacat taaaagtatc
      541 atcgattaca tgtcacattc aaatgagtgg tattacaccc attcgcgatt taacagacgc
      601 tatgttgcca aaggaatata taatgcagga agagcatata agcaaaatga gataataaca
      661 gtggctatcg acatttcagc ttccatgatg tcgcagaagg acgacataga aacagcattt
      721 ggcatagttg atgatctaac cggaaaatat aaggtgtatc ttgtttgtgt cgatgaagaa
      781 tattttatac cggtaaaaga tggtgatgca ctgatacggg caaacaggtt aaccaaaccg
      841 tatgaatata aaaagggcga ttggaagctg ataaaatcag gctatggcgg ggcaacattt
      901 ttttcgtcgt ttttcaatga atatttacaa caccatagtg agatggtact tgtcattacg
      961 gatggctaca tctatgatat tgacaaatta aaaccatacc cagctacact atggcttgtt
     1021 gcaaaaagca ggaatgaggc ttttgttcca ccatttggga aagctatacc aatggtttag
     1081 gttgctacta tggatgagca cataaatact aattataacc gggcacagtc actatatcgc
     1141 gaaggtattg aaaataccgc tcattctttg caatattttg acgatttact caactgtaat
     1201 atttcagaat ttatcattaa agcaggcagg gactggaaaa attttgatgt tgatgcagcc
     1261 atggattttt ttataagcaa taatgatatc gaagccttct atcatgcagg gatatactgg
     1321 aaaaatttta attatgaacg gggcatcact gtcatagtgg aatgggggaa tgatgaatat
     1381 attttcagag ccggaagatt ctggaagcaa tttgattata acaggggatt gtcaaaactg
     1441 atagagttgc aatctgcaaa atacatatac catgcaggat tggattggaa acagtttgat
     1501 tttgaaaagg gctttgatgc attgatgctt attggcgatc ctgaatatat tttttatgca
     1561 ggtactcact ggacagtatt taattatatg gttgcaaccg ataaactaat ttttattggc
     1621 aattgtgaat atatctataa agcgggatat caatggaaat ggtttgatta ttataaaggc
     1681 tggaaaatta tggaaagcaa gattgttgaa gggagattat ggcggggtaa ggcattgcaa
     1741 actgatgttt ggaaaaatgc attaaaaaca atatggggaa aagcaattgc caataaataa
     1801 ttggtataat ttttctatat atcaataaaa aattattgta ataatacttg acgaaagata
     1861 gtatataacg ctattct
//
LOCUS       DAOSOX010000332         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_62537_length_1862_cov_6.681240, whole genome shotgun sequence.
ACCESSION   DAOSOX010000332 DAOSOX010000000
VERSION     DAOSOX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62537_length_1862_cov_6.681240"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLX22_13040"
     CDS             complement(<1..218)
                     /locus_tag="PLX22_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOT20867.1"
                     /translation="MKVLLINPPYPFEEAPSPPFGLMALAAFLEAQGIEVKIEDYIVN
                     RFTPERASDVVSQFAPDVVGSTGVTMNI"
     gene            964..1602
                     /locus_tag="PLX22_13045"
     CDS             964..1602
                     /locus_tag="PLX22_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20868.1"
                     /translation="MNNMTQNPFKKIFEEKYEQLLQHPEMQKIKNFLSNLLSIDDQDF
                     MFMITNELGNTVTLYASKQNNNPIQSITLKLYSNADSFIPSIVFASAYDSYSIIKSYT
                     KPSNILLPECNIPLYNEVQFGKAIFHELGGCDIDEMSDVLFDVASDEEMLETIAFQMA
                     QTLTIIKICELLHDACLLLVQGDRFKKLSITKPFSFFITIDDKEYITGVTID"
BASE COUNT          640 a          315 c          283 g          624 t
ORIGIN      
        1 ttgatattca tggtaacgcc tgttgaacca acaacatccg gtgcaaactg cgacacaaca
       61 tcagaagcac gctctggtgt aaacctgttt acaatataat cttctatttt tacttcaatg
      121 ccctgtgctt caaggaatgc ggcaagggcc attaatccaa acggaggtga tggcgcctct
      181 tcaaagggat atgggggatt gataagcaat actttcatga tcttcctttt atcgtttcaa
      241 tttgatattc tcattatagt ttatgctata gcatttcata ttttatggac attaaataag
      301 caaacaattt ttggcaatat atttttgtgc ttatcataga aattatgaca tcagcatact
      361 tatgattgta ccaaacaata aattcctggc ttttcaatta tcagtgtatt attttttttt
      421 aaaaataaaa attgcaaagg gttgggcgat agttattgtg tattatacat aatccgaaaa
      481 tgacaggtga aggtgataat ggaatgcact gttgttttga tcctgcattg aagcatagtt
      541 tcctttatcg cagtgatgtt taataactca attcattaca tcttctggaa gtcactgagt
      601 atattaaggc tatgtcaatg aaaacacaat gaaatcatta ctgcatgaca caaagcaggc
      661 agtgaagtgc ataagcttat ctttgagcat cttactttta catcaacctt ttataacgaa
      721 cgctaaaaaa ataaacatga attttatgtg aaattttatt tgctataata gctactattt
      781 attaattgct tgctaaactc atttattaaa atgttaatgt aattatgata aatttttaat
      841 aaatagcttg atggaatatc tggcaagcat gttgtgctat caatgttatg aattaaatta
      901 taagcaatta taccacacta atgtcataat taagagttta aaaaaaatta tggagaggtt
      961 atcatgaaca atatgacaca aaatcctttt aaaaaaatat ttgaagaaaa atatgaacag
     1021 ctcttgcaac atcccgagat gcaaaaaata aaaaattttt taagcaatct tttatcaata
     1081 gatgatcagg attttatgtt tatgattacc aatgaacttg gcaataccgt tacattatat
     1141 gcatcaaaac aaaataataa tccaattcag tctataactt taaaactata cagcaatgca
     1201 gattctttta ttccttcaat agtgtttgcc tcagcatatg attcatatag tataatcaaa
     1261 tcatacacaa aaccatctaa tatactgcta cccgaatgta acatcccgct atataacgag
     1321 gtacaatttg gcaaggctat cttccatgaa ctgggaggtt gcgacattga tgagatgtct
     1381 gatgttttat ttgacgttgc ttcagatgaa gaaatgctag aaacaatagc tttccaaatg
     1441 gctcaaacgt taacaataat caaaatatgt gaactgcttc acgatgcgtg cctgctactg
     1501 gttcagggcg acagatttaa aaaactatcg ataacaaaac ctttttcatt ttttataacc
     1561 attgatgata aagaatatat taccggtgta acaattgatt aatcagtaat cattgatagt
     1621 accatgattt gtgcgatagt tcctttatat catcgtggtt tcacaggtat ttgctcatca
     1681 ataataaatc ttcctttgaa aacccaaaca gcactattaa cctggtcccg gcgaagctgt
     1741 ctaaaagttg atttttacag ccccattgtt aacaaacaca aatacatatc cctattaaac
     1801 catagtagaa tcgttattgt ttgctcctct ttacttcttt ataaaccctg ttgaaccacc
     1861 tc
//
LOCUS       DAOSOX010000333         1860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_62616_length_1860_cov_6.344044, whole genome shotgun sequence.
ACCESSION   DAOSOX010000333 DAOSOX010000000
VERSION     DAOSOX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62616_length_1860_cov_6.344044"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..646)
                     /locus_tag="PLX22_13050"
     CDS             complement(59..646)
                     /locus_tag="PLX22_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT20869.1"
                     /translation="MKNAKSFQDIKYTEKENKKKIIIDAAERVFATKPFNQVTMRDIA
                     KEAGISAGAIYRYFPDQQALFVEAFLLGAEDIINEMEIIIATSKNPLKDTIYTFLSYL
                     NKHDPYFRMMTHFMLDANLSPDLINKLNEVERRVLDQFTKLFASRTNARLLAHSLFAS
                     LNGVMITFHNYPGRSKKEVRYHMNRLGETLYQLFS"
     gene            complement(1045..1117)
                     /locus_tag="PLX22_13055"
     tRNA            complement(1045..1117)
                     /locus_tag="PLX22_13055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1082..1084),aa:Asn,seq:gtt)
     gene            complement(1429..1737)
                     /locus_tag="PLX22_13060"
     CDS             complement(1429..1737)
                     /locus_tag="PLX22_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20870.1"
                     /translation="MKRFVILFTILIAVFIAAAIYSTNRVETEPAYSASPVDCGWDAV
                     KPHFVNDSPCPGAVYCSYGNCGEYCCPWGYFYSNPCTCRCYRTSYDAGQDCNSYFRCN
                     "
BASE COUNT          597 a          329 c          315 g          619 t
ORIGIN      
        1 tgaatttcag aattgtgcat tgcatgaggt tgaagctgca agggaagatg ctgcgttatc
       61 aggaaaataa ttggtataat gtttcgccta atctattcat gtggtagcgt acctcttttt
      121 tacttcggcc ggggtaatta tgaaatgtaa tcataactcc atttaatgat gcaaataagc
      181 tatgcgctaa tagtcgtgcg ttagtacgtg aagcaaaaag ttttgtaaat tgatccagca
      241 cccggcgttc gacttcattg agcttattta ttaaatcggg actcaagttt gcatccagca
      301 tgaagtgagt catcatgcga aaataaggat cgtgcttatt taaataactt aaaaatgtat
      361 agatggtatc tttaagagga tttttacttg ttgcaataat tatttccatt tcattaatta
      421 tatcttctgc tcccagtaaa aaagcttcaa caaacagcgc ctgttgatca gggaaataac
      481 ggtagatagc acctgccgag atgcctgctt cttttgcaat atcacgcatt gttacctgat
      541 taaaaggttt ggtggcaaat acacgctcgg cagcgtctat gattatcttt tttttgtttt
      601 ctttttcagt atatttaatg tcctgaaagg attttgcatt tttcatgcca gaattgttac
      661 ttagaaacta caatttgtca agatataatt tgtttatgta ttattgtacc atatccataa
      721 ttttcaaaaa atataaacac tgtgacaatg gtacaacgaa acatttttaa acggattgca
      781 gatgaaatct gctgttttgg tggttagctt cagtaaataa atgttactat actccaatac
      841 ctctattgta taatttgttt tctctttttc gttgcttttc taccttataa atacatctca
      901 gtacaaacta atatacattt ttcttgtaaa cgtttagtag aagtagagcc tttatacaat
      961 acttattgca ttaaaaaatg tgttaatcga gattatgaat gggattgata aaaaaaagtc
     1021 atgctcatta tggttttatg tatgctcccc cggcaggact cgaacctgcg acctggtggt
     1081 taacagccac tcgctctacc gactgagcta caggggaatg gtataacctg caccatggta
     1141 attaatccca acaaagctgt gattttacca tttaaactac aggatgatag taaaaattaa
     1201 tgtactaact gaaacaatat tttcaaaaaa aattatgtaa aaactttcat tttaaagacg
     1261 aggctattag atttatacta tgtaacttgt caataaaaat tttgcctttt aattttgctt
     1321 ttaaaaataa aatctttatc aaaagcatac aggtcgtcaa aaacttattc aggataataa
     1381 atgtattgac atggtaatat aatagtaaat tcatataaga cagatatact aattacatct
     1441 gaaatagcta ttgcaatcct gccctgcatc atagcttgta cgatagcatc ggcatgtaca
     1501 tggattggaa tagaaatatc cccacggaca gcagtattca ccacaatttc catacgaaca
     1561 gtacaccgct cctgggcagg ggctatcatt cacaaagtgt ggcttaactg catcccaccc
     1621 gcagtcaaca ggcgaagcag aatatgcagg ttctgtttca accctgttgg ttgaataaat
     1681 tgctgccgct atgaagacag ctatcaggat ggtaaacaat ataacaaagc gtttcataag
     1741 agcctcccgc taaagttata attttctaca tactagtatg tatattaata ttactatgcg
     1801 tatagtattt gtcaacctat ttttccccac taccattacc attattatac tgctctgcaa
//
LOCUS       DAOSOX010000334         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_63966_length_1833_cov_8.790214, whole genome shotgun sequence.
ACCESSION   DAOSOX010000334 DAOSOX010000000
VERSION     DAOSOX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63966_length_1833_cov_8.790214"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PLX22_13065"
     CDS             <1..1076
                     /locus_tag="PLX22_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT20871.1"
                     /translation="KSKLKAAAIYDTVPPDSEIKMVNELAKMYTSKKCDSIIAVGGGS
                     VLDTAKGVNIIVSENASDLMQFAGYGNVKRKLKPLVAVPTTAGTGSEVTSVAVIANHE
                     KNIKMIFGSAFLLPDIAIIDSRMTKTLPPTITAFTGMDALTHAVEAYFCLAKNPLSDT
                     TALAAIQIIAQNLVNVVKKPSDTDGRLALALASNLAGMAFANSMVGMIHNIGHAVGGV
                     CGVPHGVCMNILLPYGLEYNMHKASQYIGELLYPLAGDEVYNATKKKERPQKTVEYIR
                     NLIQDLHDATDGKHPRFFKEVVDKTGKQMVPKEKLVDIAKTAMSDPAQFYNPEDLDYN
                     DYMMVLEAAWEGTPLDKKKIKKG"
     gene            1140..>1833
                     /locus_tag="PLX22_13070"
     CDS             1140..>1833
                     /locus_tag="PLX22_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20872.1"
                     /translation="MKVECNNIDSTIKNFTSQFILRTEGISYFIIVPVLLLFLVTTLS
                     ISHKQLFYIAVCTAFAFPISFITTQLNNIIATRPIQRYFNGIVKNEDIPDEMYNAAFK
                     RLLALPYIHSIGAFFRWVFGLSMFTVPLTVLNLFKPNEVFIIWSSILIAAPSGTVLYF
                     LLTEIFIQNIYNKGIFPKIPPNFTSKYRMNITIKLILSITIIVFTPFFIMIAFIASIT
                     NQPTFSLSGIWWQ"
BASE COUNT          584 a          335 c          337 g          577 t
ORIGIN      
        1 aaaaatcaaa attaaaagct gccgccatct atgatactgt tcctcccgat tcagagatta
       61 agatggtaaa tgaactggct aaaatgtata catcaaaaaa atgtgattct attattgctg
      121 ttggtggtgg ttcggttctt gatacagcaa agggcgttaa tataatagtt tcagaaaatg
      181 cttcagattt gatgcagttt gcaggatatg gcaatgttaa gcgtaaactt aaaccgttgg
      241 ttgcggtacc caccacagca ggaacaggat cggaagtaac atccgttgct gtcattgcca
      301 accatgaaaa aaatattaaa atgatatttg gttcagcatt tttactgcct gatattgcca
      361 tcattgattc aaggatgaca aaaacattac caccaactat caccgcattt actggaatgg
      421 atgcgctcac ccatgctgtt gaagcatatt tttgtttggc caagaatcct ttaagcgata
      481 caacagcatt agcagcaatc cagattattg ctcaaaacct tgtcaatgtg gtaaaaaaac
      541 cttctgatac tgatggacgc ttagcactgg ctcttgcttc aaatctggca gggatggcat
      601 ttgcaaattc catggttggt atgattcata acattggaca tgccgtaggc ggtgtatgtg
      661 gcgttcccca cggtgtatgt atgaatattc ttctgcccta tgggttagaa tacaacatgc
      721 ataaagcttc acagtatatt ggcgagcttc tttatccgct ggcaggtgat gaagtgtata
      781 atgcaacgaa aaagaaagaa cgtccgcaaa aaacagttga gtatattcgc aatcttattc
      841 aagatctaca tgatgcaact gatgggaaac accctcgttt ttttaaagag gtggttgata
      901 aaacaggtaa gcagatggtc cctaaagaaa aactggttga tattgcaaaa acagccatga
      961 gcgatcctgc tcaattttac aatcccgaag atttagatta taacgattac atgatggtcc
     1021 tggaggctgc atgggaagga actccacttg acaaaaagaa aataaaaaaa ggatgataaa
     1081 aatatgtctc agcgctattg tagcgaagag cgctgagaca tagtatagag ggattgtgta
     1141 tgaaggttga atgcaataat atagattcca ctattaagaa ttttacgtct caatttattt
     1201 tgcgtacaga aggtattagc tattttataa tagtaccagt attattactt tttttagtga
     1261 ccaccctgag catatcacac aaacagctat tttatattgc tgtctgtact gcttttgctt
     1321 tcccaatatc atttatcaca actcaattaa ataatattat tgctaccaga ccaatacaac
     1381 gatattttaa tggtatagtt aaaaatgagg atattcctga tgaaatgtat aatgctgcat
     1441 ttaaacgttt attagcgttg ccctatattc atagcattgg tgcatttttc cgctgggtat
     1501 ttggtttgtc aatgtttacc gtccccctta ctgtattaaa tctttttaag ccaaatgaag
     1561 tttttattat atggagcagc atccttattg ctgcgccttc tggcactgtt ttgtattttc
     1621 ttttgaccga aatttttata caaaatattt ataataaagg catttttcca aaaattccac
     1681 caaacttcac atccaaatac aggatgaaca ttaccataaa attaatatta tcaattacta
     1741 ttatagtctt caccccattt tttatcatga tagcattcat cgcttccatt accaatcagc
     1801 caacattcag cctgtcagga atctggtggc aaa
//
LOCUS       DAOSOX010000335         1829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_64149_length_1829_cov_5.279030, whole genome shotgun sequence.
ACCESSION   DAOSOX010000335 DAOSOX010000000
VERSION     DAOSOX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64149_length_1829_cov_5.279030"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..468
                     /locus_tag="PLX22_13075"
     CDS             244..468
                     /locus_tag="PLX22_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOT20873.1"
                     /translation="MNWKDRITIDPNICHGKPCIKGTRIIVSVILDNLANGMKEEDIL
                     SSYPSLTSEDIQAAIAYAAELSHERLILIM"
     gene            complement(457..1371)
                     /gene="dusB"
                     /locus_tag="PLX22_13080"
     CDS             complement(457..1371)
                     /gene="dusB"
                     /locus_tag="PLX22_13080"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOT20874.1"
                     /translation="MNTTFPIHVKSKLFLAPMAGYTDLPFRLIAHTSGAGMVFSELLS
                     ADGIVRSNKKTLAMLSFSDEEKPLGIQIFGNDPAVMAEAAVRLLSHSPDCIDINAGCC
                     APKVCNNGKGAGLLNNVELLHRIACAVVKSVDIPVSAKIRLGFTQGTKNYREVVAALE
                     QAGVSFITVHGRTRDQFYSGKADWDAINTIADFSRVPIVGNGDITSYEDAMEKLSTAG
                     CAAVMIGRAAIGNPWIFCGQQPSYQEKIAMIQKHYERMVQHYGNYGIILMRKHIVNYL
                     KGFPGAVKLRKKLLTLTSMDEILDVLHY"
BASE COUNT          574 a          340 c          357 g          558 t
ORIGIN      
        1 acggaaacat tagccatgcc acgcctttca atgtatggag taagggaaca ggattgtgct
       61 acaatagtaa aagaagctga taacaaaaat aatccggtta agctgaatga tgatgaaatg
      121 ataggattat tgcgtgaaag gttatgatat ctgatatttt attgatgtag aagttattaa
      181 aaatgaaaaa taattgcttt tacaattgtt gtatagtaag cttatatgta tcaggaggta
      241 agtatgaatt ggaaagacag gataacaatt gatccaaaca tatgtcatgg aaaaccatgt
      301 attaaaggta caagaattat tgtttcagtt atattagata atttagccaa tgggatgaaa
      361 gaagaagata tattgagtag ttacccctca ttgacatctg aagatataca agcagctatt
      421 gcttatgcag cagaattatc tcatgaaaga ttgattctaa taatgtaata catccagtat
      481 ctcatccatt gaagtgagtg ttagtagttt cttccgcagt ttaactgcac cgggaaatcc
      541 ctttaaatag tttacaatat gtttccgcat gagtattatg ccataattgc cataatgctg
      601 caccatacgt tcataatgtt tttgtatcat agctatcttt tcctgatatg atggttgttg
      661 cccacaaaat atccatggat tgcctatagc ggcgcgccct atcatcacgg cagcacaacc
      721 ggctgtggaa agtttttcca tggcatcttc atagcttgtg atgtcgccgt taccaacaat
      781 agggaccctg ctaaaatcag ctattgtatt tattgcatcc caatctgctt ttccgctgta
      841 aaactggtcg cgcgtcctgc catgcaccgt aatgaatgat acccctgcct gctctaatgc
      901 tgcaacaacc tccctgtaat ttttggtgcc ctgcgtaaag ccaagccgta ttttggctga
      961 aaccggtatg tcaacgcttt tcaccaccgc gcatgctatc ctgtgtagca gttcaacgtt
     1021 gtttagtaaa ccggcgcctt tcccgttgtt gcacactttt ggagcacaac aaccagcatt
     1081 gatatcaata caatcagggc tgtgcgatag taacctgaca gcggcttctg ccattacagc
     1141 agggtcattg ccaaaaattt gaattcccag cggtttttcc tcatctgaaa acgatagcat
     1201 ggcaagagtc tttttattgc tgcgaacgat accatccgca cttaaaagct ctgagaatac
     1261 catccctgca ccagatgtat gggctatcag cctgaatggc agatcagtgt aaccggccat
     1321 gggagcaaga aaaagtttac tttttacatg tataggaaat gtagtgttca tataacgata
     1381 atagctcttt gaatgctatc aataaaaaaa ataatctgta ttggtttagc aaaaacttgt
     1441 caaccacaga taagaatgca gcgcatgtca ttctgaattt atttcaggat ataatattac
     1501 atatttagat tccgggtcaa gcccggaatg acaggagtag aactacgtcc atcaaaatga
     1561 cgaaatggtg gaataattcg atggttgggc aaaaggtgaa aaaaaatttt ttatgcttga
     1621 ttatttagca tgctacatat acaaaaagta atgaaagata atgatagggg gttttactgt
     1681 tatgaagaaa tgtatagcac taatgtgtat gttttttatt cttactgtgt cagtatatgc
     1741 ccaggatatt acaattatac aaacaaccga tttgcattcg cattttttag gtttctcgcc
     1801 caatattgat tatacacctg caaccatta
//
LOCUS       DAOSOX010000336         1824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_64378_length_1824_cov_5.456755, whole genome shotgun sequence.
ACCESSION   DAOSOX010000336 DAOSOX010000000
VERSION     DAOSOX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64378_length_1824_cov_5.456755"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLX22_13085"
     CDS             complement(<1..183)
                     /locus_tag="PLX22_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT20875.1"
                     /translation="MIIEQLLVSPMDVFCYLLVCPITKEAVLIDPAADFDTIMQAIRK
                     HNAHVKYIINTHGHFDH"
     gene            830..1654
                     /gene="asd"
                     /locus_tag="PLX22_13090"
     CDS             830..1654
                     /gene="asd"
                     /locus_tag="PLX22_13090"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOT20876.1"
                     /translation="MLLFFKIIPTRLLSRIFGIAAQIPLPQSILKKLIVWYSKKFNVL
                     LEEAHIPPGGFRTFDEFFTRNLKRGMRTIDPDIYSLISPVDAMVSSYGDIVYGQIVQA
                     KGINYDVASLIPGVMESTFIGGSFTTLYLSPGDYHRIHAPVTGTITAYQYDPGRLYTV
                     QDWLVNMLPGLFTVNERVTTYIQSAHGLVAVCKVGAMNVGRITLSYAPLYTNRFCAKP
                     YFYIMDRHKQVTIQKGDELGTFHLGSTIIVLTQKRCRFLPAIAGKKVTMGQAIAFF"
BASE COUNT          585 a          274 c          357 g          608 t
ORIGIN      
        1 gtgatcaaaa tgtccatggg tgttaatgat atattttaca tgagcgttgt gtttacgtat
       61 tgcctgcatg attgtatcaa aatcggctgc cgggtctata agcaccgcct cttttgttat
      121 ggggcacacc agcagataac aaaatacatc cattggactt accagaagct gttctataat
      181 cataactttt cctttaatga aaatagttta ttctaatagt gcaatacttt ataactgttt
      241 actttagtac catagtgagt aaccgtaatg tttaataagc agcataattt attataaaaa
      301 agatatagaa tcaggtagtt actttaaatt cattcagatt aaagaatgat attggatgtt
      361 ccataataaa aatcaacatg atagtaattt ttacaagaat ttttttataa aaacagacca
      421 tatggccaat ttttaattgg gtaatttgtt taaacattac aattagtttt tatacaccgg
      481 atgagtaaaa ataaggaagg aaatattttt gaggattttt atattatttg ttaatatatg
      541 tgtatataat tatggcattt gtaaaaattg taggcatggc attgcgtatg ctgtgatgat
      601 tgatactata agctgttttg ttgaagttag agaatgaata acgcttgatt gaatactgat
      661 ataagagttg tcatacatat actgatttgt ttgtactttt gcaaaggata gctaaatata
      721 aatagacaat caaataaaag gaattgactt gattccatag tattgtaatc tattatgcat
      781 ggtagtaata atttaatttt tgggagatac aacattgaaa ggattgccaa tgcttctgtt
      841 ttttaagatt ataccaacac gtcttttatc acgaattttt ggaattgcag cgcaaatccc
      901 tctgccgcaa agtatactca aaaagcttat agtctggtat agtaaaaaat tcaatgtatt
      961 gcttgaagaa gcacatatcc cgcctggtgg attcagaacc tttgatgaat ttttcacaag
     1021 gaatctaaaa agggggatgc gtaccattga tcctgatata tattcgctga tttcacctgt
     1081 tgatgcaatg gtgagttcct atggtgatat tgtatatggg cagattgttc aggcaaaagg
     1141 catcaactat gatgttgcat ccctcattcc cggtgtaatg gaaagtacat ttataggcgg
     1201 ttcatttact acattatacc tatctccggg tgactatcac cggatacatg caccggtcac
     1261 tggaacaata acagcttatc agtatgaccc tggaaggcta tacaccgtgc aggattggct
     1321 ggtgaacatg ctgccaggac tttttacggt taacgaacgt gtaaccacct atatccaatc
     1381 agcgcatgga ttggtagcag tgtgcaaggt tggtgcaatg aatgttggga ggataacgtt
     1441 gagttatgct ccattatata ccaacaggtt ttgtgctaaa ccatacttct atataatgga
     1501 cagacacaaa caggtaacaa tacaaaaagg tgatgaactg gggacttttc atcttggctc
     1561 taccattatt gttttaacgc aaaaacggtg cagattttta cctgcgattg caggtaaaaa
     1621 agtcaccatg gggcaggcaa tagcgttttt ttaagatatc gtaccaaact gttatatgta
     1681 tatggcagat gctgacggta acaatctgtt tattttccac ttgctatttt ttgttaattt
     1741 tagtatagta taggtggtgg cttgtataga ggaggaacat ctatgaaaga tgttgctgta
     1801 aaagaaagcg ctatttttaa acaa
//
LOCUS       DAOSOX010000337         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_64782_length_1816_cov_5.381034, whole genome shotgun sequence.
ACCESSION   DAOSOX010000337 DAOSOX010000000
VERSION     DAOSOX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64782_length_1816_cov_5.381034"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLX22_13095"
     CDS             complement(<1..634)
                     /locus_tag="PLX22_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT20877.1"
                     /translation="MKKSLFKKGAYIYIEGDEDADTVYIVDKGVVQLSRYNKNAPIYK
                     NILKGGEFFGVTASLCNKPRMESAIALTDSIITSLTRQEFINLLSKTPQIAIKVLNYF
                     SDELRAYNEMMIALKDKEVELETPEEALYNHINYFYKNNSYAYALYCCKRYGELYPGG
                     MFATAVKSIMEKIKGKVKGSIVPVNEPPYKIFMDQQMIFCEDEPGNEMYII"
     gene            complement(641..1189)
                     /locus_tag="PLX22_13100"
     CDS             complement(641..1189)
                     /locus_tag="PLX22_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOT20878.1"
                     /translation="MKIIVPLAPGCEEIEAITIIDVLRRANLNVVSAGLTDSLITGSH
                     GIAIKPDILLHKANPVDYNCIVLPGGMPGSENLKKDDDVIKFIKHIFSKGGYVAAICA
                     APIVLGSAQILYGKKATCFPGLEGELIGATLINEPVVVDGTIITAKGPACAIPFALTL
                     AGLFAGKDVQDTLKNAMQVYWM"
     gene            complement(1512..>1816)
                     /gene="pth"
                     /locus_tag="PLX22_13105"
     CDS             complement(1512..>1816)
                     /gene="pth"
                     /locus_tag="PLX22_13105"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT20879.1"
                     /translation="SYYKELPDNLIVLHDDIELTFGNVALKFGGGHKGHNGIRSIQQH
                     VGTPDFYRIRIGVGRPSDAIPVADYVLSDFTQEEKSYCEDIIHKTNSILIQCLHQK"
BASE COUNT          559 a          391 c          286 g          580 t
ORIGIN      
        1 tgatgatata catttcattg ccaggttcat cctcgcaaaa gatcatctgc tgatccataa
       61 atatcttata cggaggttca ttaacaggaa ctatcgaacc ctttaccttt ccttttatct
      121 tctccattat gcttttgaca gcagtggcaa acattccacc gggatataat tctccataac
      181 gcttgcagca gtacaatgca tacgcataac tgttattttt ataaaaataa ttgatgtgat
      241 tatacaatgc ctcttcaggt gtttccagtt ccacctcttt atcctttaag gcaatcatca
      301 tctcattata tgcgcgcagc tcgtccgaaa agtagtttaa cacttttata gcaatttgag
      361 gtgtctttga taaaagatta ataaattctt gcctggtcaa tgatgtaata atgctatcag
      421 taagtgcaat agccgattcc atacggggtt tattgcaaag ggatgcagtg acaccaaaaa
      481 actcgccacc ttttaatatg tttttatata tcggtgcatt tttattataa cggcttaact
      541 ggacaacccc tttatctaca atgtaaacag tatcagcgtc ttcatcacct tcgatataaa
      601 tatatgctcc cttcttaaaa agactctttt tcatatatca ctacatccaa taaacctgca
      661 tggcattttt taatgtatcc tgaacatctt tgccagcaaa aagtccagct aatgtaaggg
      721 caaatggtat agcacaagca gggccctttg ctgttataat ggtaccatct actacaaccg
      781 gttcatttat taatgttgca ccaataagct ctccttccag tcccggaaaa caggtagctt
      841 ttttaccata taaaatctgt gccgacccca acacgatagg agcagcacat attgcagcaa
      901 catagccgcc ttttgaaaaa atatgtttaa taaatttaat aacatcatca tcttttttta
      961 aattttcact ccctggcatt cctccaggca acactataca attataatcg acaggattgg
     1021 ctttgtgcaa tagaatatca ggttttattg caataccgtg cgaccccgtt atcagtgaat
     1081 cggttaaccc tgctgaaaca acattaagat ttgctctccg cagtacatcg attatggtaa
     1141 tggcttcaat ctcttcacat cctggtgcaa gtggcacaat aattttcata attttacctc
     1201 ctatataatt ataacatgga atatcttttt ctaccttcgt cattctgatt agcacagtgc
     1261 tttatgtcat tctgatggag cattcgcaag catagaaact attcttttat aaaacgacga
     1321 gatttttcac tctgttaaga atgacatgac catacaacaa cacgattgtt cagaataact
     1381 ttctacggct cagaatgaca ttgctgttta gctgtaccag ccgtctgctt tactgtagca
     1441 gcaatgcata ctatacctat agcataccat gaaagggcaa taagtcaagt acatgtaact
     1501 atcgctcata attatttttg atgtaagcat tgaatgagga ttgaattagt tttatgtata
     1561 atgtcttcgc aataactttt ttcttcctga gtaaaatccg aaagaacata atctgccacg
     1621 gggatggcat cggaaggcct tccaacgcca atacgtattc ggtaaaaatc aggggtacca
     1681 acatgctgct ggattgaacg gattccatta tgccccttat gacccccacc aaactttaaa
     1741 gcaacattac caaacgtaag ctctatatca tcatgcagaa caattaaatt atcaggcaat
     1801 tctttgtaat atgaca
//
LOCUS       DAOSOX010000338         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_65151_length_1808_cov_5.132345, whole genome shotgun sequence.
ACCESSION   DAOSOX010000338 DAOSOX010000000
VERSION     DAOSOX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65151_length_1808_cov_5.132345"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..>1808)
                     /locus_tag="PLX22_13110"
     CDS             complement(274..>1808)
                     /locus_tag="PLX22_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOT20880.1"
                     /translation="SLTILSSKKNIKGDTMWHFAPTNVHWIDITLIVLYLAGITVFGF
                     YYKSHIKTAKDYFLANRSLPWWVIGLSIIGTNIGSNDYIGAAGNAYSIGIAQANFEWI
                     GAIPAMILSALIFIPFFWRAGVYSIPEYLGLRYNNIVRFIAACVMSIFSVVIVGVFLW
                     ATAIMLQTYLGWPVWFSIFITATVVGFYTISGGLGAVAITDTIQVFIMFFSAIIVAVI
                     GIKTIGGFDSFIETLKVNYPDHLNAFLPANHPAFPWHGVILGLGLVLSPAYWCANQAI
                     LQRTFGARSEWDGKASLIFAAMLKTFVPFLIVMPGLLALTLAGKSITHQDQALPWVIK
                     NVLPAGISGLMFVAFIAALQSSIDSTMNSTSVMIVRDIINVVRKEKLSDALQLKFGRI
                     FTFCILIIGVIFAPITAYFQGIYVFIQYALSLFQGPIFALMIFGILDKRITATAGVVS
                     LLTGLCFAALLGWFHLNMLYIAFFSFCYSAIVLYIISTFTKKKSLSELKNVVYSSVVN
                     IK"
BASE COUNT          646 a          344 c          325 g          493 t
ORIGIN      
        1 tgttgggtgc accctccatt acaaaatcat aggggagcat ccatgcaata atgagaatgc
       61 ctgaaaagat gatcatagca gcaatctttg ctgtccacag tggtaacgaa tagagtatct
      121 gtaacatata aattccctct taagatatga ttatgcaatg caatatatac atcaatactt
      181 actgatctac catggtaatt ggattattaa tcatgcaaca gaaattagca ataaaggctc
      241 aatcataaac tatataaatg tgttcagtac tattcacttg atattcacaa cactactata
      301 tacaacattt tttaattcag ataatgactt cttttttgta aatgtgctta taatatagag
      361 aactatcgcc gaataacaaa aagaaaaaaa agcaatatac aacatattga gatgaaacca
      421 ccctaacaat gcagcaaaac atagtcctgt tagtaaacta accacgccag cagtagctgt
      481 tatacgttta tctaaaatac caaagatcat taatgcaaat atgggacctt gaaaaagaga
      541 cagcgcatat tgtatgaaaa catagatacc ctgaaagtat gctgtaatgg gagcgaaaat
      601 aacccctatt ataaggatgc agaaagtaaa tattcttcca aatttaagtt gcagtgcgtc
      661 tgataatttt tctttgcgta ctacgttgat gatatcacgt acaatcatga cagacgttga
      721 gttcattgtt gagtcaatag atgattgaag tgctgctata aacgcaacaa acatgagtcc
      781 agaaatgcct gcgggtaaaa catttttaat aacccatggc aatgcctggt cctgatgagt
      841 gatagatttt ccagcaaggg ttagagcaag taacccgggc ataacaatta aaaatggcac
      901 aaaggtcttg agcatagcag caaaaatcag tgaagctttt ccatcccact cagagcgtgc
      961 gccaaatgtt cgttgtaaga tagcctggtt ggcacaccaa taggctggtg acaaaaccaa
     1021 tccaagccca agtataacgc catgccatgg aaatgcagga tggtttgccg ggaggaaagc
     1081 attaagatga tcaggatagt taaccttcag tgtttcaata aatgaatcaa atccaccgat
     1141 agttttaatt ccaataacag caacaataat tgcactaaaa aacatgatga atacttgaat
     1201 tgtatcggta attgcaacag cgccaagtcc acctgaaata gtgtaaaatc ctactacagt
     1261 tgcggttata aaaattgaaa accatacagg ccagcctaaa taggtttgta gcattatagc
     1321 tgttgcccac aagaacacac ctacaattac aactgaaaag atactcatga cacatgctgc
     1381 aataaaacgc acgatattat tatagcgcaa tcctagatac tctggaatgg agtatactcc
     1441 tgcacgccag aaaaacggta tgaaaataag tgccgatagt atcatggcag ggatagcccc
     1501 tatccattca aagtttgcct gtgcaatccc tatgctgtag gcgttaccag cagcgccgat
     1561 gtaatcattt gaaccaatat ttgttccaat gatagaaaga cctataaccc accatgggag
     1621 cgacctgttt gctaaaaaat aatcttttgc agtttttata tgtgatttgt aataaaatcc
     1681 aaaaacggta atgcctgcta aataaagtac gatcagtgta atatcaatcc agtgaacatt
     1741 ggtaggtgca aaatgccaca tagtatcccc ttttatattt tttttgcttg ataatatcgt
     1801 taacgatt
//
LOCUS       DAOSOX010000339         1807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_65202_length_1807_cov_4.679795, whole genome shotgun sequence.
ACCESSION   DAOSOX010000339 DAOSOX010000000
VERSION     DAOSOX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65202_length_1807_cov_4.679795"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1807
                     /locus_tag="PLX22_13115"
     CDS             <1..>1807
                     /locus_tag="PLX22_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT20881.1"
                     /translation="LIMAIVTAMGGIIPFVAPYYYATQISVLMAAIEAIIAIIIGIVA
                     QIKKVKEALYFNVATFSFVAGVILIVLRSFGMLGDNFFTAWGYQLGSSAMVILFSLGI
                     ADKVNTWRKDKERALSQLATSEAKYRTLVENAHDGIMLVQNWNMVYSNNALARMLGYT
                     VEELLNKNINFITADSSLGQEVIIQYENRMLGKAAKLQYEAQLKRKTGTVMDVLISAQ
                     KVILDGISTSIAIITDISSLKEAHYTIQQQYEEIQSQYEELEAMNEEMVRTHYELTEA
                     THNLEEEKERLHATLRSIYDAVIATDIQGKIIIINARAEQLLGNISTEVIGNQFVTVF
                     PLRYTTMEEFKNPVNEILRYGSLHTAGEPLVLQLHTKEIIVELSGAPIYVEGKIIGTV
                     IVLHDITQQYWMEKEMHKISKLESLSILAGGIAHDFNNLLTAIIGNLSILGMKLSENT
                     VYQKYIERIENAAQRAVDLTKQLLTFSKGGEPIKAVASLEQLITDTTQFVFSGSNVRF
                     DLDIQPDLWSSEVDIGQISQVFQNIMINAMQSMQQGGIVRIKAHNYVYDGKSQKPLLP
                     GDYIAITIKDEGIGIPKENLSKIFDPFFTTKEHGSG"
BASE COUNT          572 a          310 c          401 g          524 t
ORIGIN      
        1 tacttataat ggcaattgta acggctatgg gtggtattat cccctttgtg gcgccctact
       61 actatgcaac gcagatttct gttttaatgg cagctattga agccattatt gccattataa
      121 taggtattgt tgcacagatt aaaaaagtaa aggaagctct gtattttaat gttgctacat
      181 ttagctttgt agcaggtgtc atcttaatag tattgcgttc atttggcatg ctgggcgata
      241 acttctttac ggcatgggga tatcagttag gctcttcggc aatggttatt ttgttttcat
      301 tggggattgc ggataaggtt aacacgtggc gtaaggataa ggagcgtgct ttatcgcagc
      361 ttgcaacctc agaggcaaag taccgtaccc tggttgagaa tgctcatgat ggcattatgc
      421 ttgttcagaa ctggaacatg gtatatagta acaatgcact tgcacggatg cttggatata
      481 ccgtagaaga attgttaaat aaaaatatca attttatcac tgctgattca tcgctggggc
      541 aggaggtaat aatacaatat gaaaacagga tgcttgggaa agcagctaag ttgcagtatg
      601 aagcgcagtt aaagcgtaaa acaggcaccg ttatggatgt attgatttca gcacagaagg
      661 ttatactgga tggtatatct acaagcattg ctatcattac cgatatctct tccttaaagg
      721 aggctcacta cacaatacaa cagcaatacg aagagataca atcacagtat gaagaactgg
      781 aagcaatgaa tgaagagatg gtacgcacgc actatgaact tactgaagcc acccataatt
      841 tagaggagga gaaagagcgt ctccatgcta ccctgcgatc tatatatgat gccgttattg
      901 caacagatat acagggaaaa atcattatta tcaatgcgcg cgctgagcaa cttttaggga
      961 atataagtac tgaagtcata gggaatcagt ttgttactgt atttcccctg cggtacacta
     1021 ccatggaaga atttaaaaat cctgtgaatg agatattacg ctatgggtcg cttcatactg
     1081 ctggagaacc actggtgttg cagttacata caaaagaaat tattgtggag ctatcgggtg
     1141 cccccatcta tgtggaagga aagatcattg ggactgtcat agtactgcat gatattaccc
     1201 agcagtactg gatggaaaaa gagatgcaca aaataagtaa attagaatca ctcagtattt
     1261 tagcaggtgg cattgctcat gattttaata atctattgac tgctattatt ggaaatttat
     1321 caattcttgg aatgaagctg tccgagaata cagtatatca aaaatatatt gaaaggattg
     1381 aaaatgccgc tcaacgcgct gttgatctta ctaagcagct tttaaccttt tcaaaaggtg
     1441 gcgagccaat aaaagcggtt gcttcgttgg agcagttgat taccgataca acacaatttg
     1501 ttttttcagg atcaaatgtg cgctttgatc ttgatattca gcctgattta tggagtagtg
     1561 aagtagatat tggtcagata agccaggttt ttcaaaatat tatgataaat gccatgcaat
     1621 caatgcaaca ggggggcatt gtcaggatta aagcgcataa ttatgtctac gatggcaaat
     1681 cccaaaaacc acttttacca ggggattata ttgccataac cattaaggac gaaggcatag
     1741 gcataccaaa agaaaatttg tctaaaatat ttgatccatt ttttaccaca aaagagcatg
     1801 gcagcgg
//
LOCUS       DAOSOX010000340         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_65460_length_1802_cov_8.718374, whole genome shotgun sequence.
ACCESSION   DAOSOX010000340 DAOSOX010000000
VERSION     DAOSOX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65460_length_1802_cov_8.718374"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /gene="pilM"
                     /locus_tag="PLX22_13120"
     CDS             complement(<1..1577)
                     /gene="pilM"
                     /locus_tag="PLX22_13120"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOT20882.1"
                     /translation="MFENLAVFDIGSSSIKCIKAKTGFKNFQIESLIIEKLDLSVEDK
                     FNRIEIAIDKIISNNDFTDYTIITTLPSNKLFYYSLNFPFNNIEQISEVIHYEIADCI
                     PLDIESVIYDFQPVEIKTSNGMDVIAVVSQKSLINKLIDLFSTYNLAITFIGSEHNAL
                     YHSYVYFNTVQDESVVQLNIGYEKSIINLVINNELIATRCLLFGVKNLVQHFEKYKPE
                     NVALQEIFSYLSFDFSSIENNIHFELPKKLNITQQKFKSLFNELQEEINNLIHEVVLF
                     LNVEINKSANITIQRIIISGGGAAIAGLSSLISQNLQIPVVMQALINQTNETELQSQF
                     FIAFGTLINYIHRNKHKTIDFLKDEFGTSLKLKSKKNFYIAFFYGSLSIIFFIILIII
                     LLVNQVSTSSYFKTILEERYKRYFLTSTIPDDPIKEATNKYTMVKREVDSIESFLKIE
                     EKMIDILYLLVSNFPYDANFDLNNLVINESIIRLDGSINSIAKIDEFKNRLTQTQKFD
                     SIVFNTNVMQNNVKFSMT"
     gene            complement(1593..>1802)
                     /locus_tag="PLX22_13125"
     CDS             complement(1593..>1802)
                     /locus_tag="PLX22_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20883.1"
                     /translation="LIRRRLQKPFKNVDEISDIITDENVRKNLLTVRSYIFQIRSIGK
                     VGSTTLSIIIFYHRERKQIIFWSEA"
BASE COUNT          636 a          243 c          238 g          685 t
ORIGIN      
        1 gtcattgaaa atttgacatt gttttgcatt acattggtgt tgaaaacaat agaatcaaat
       61 ttttgagttt gtgttaatct atttttaaac tcatcaattt ttgcaataga atttattgat
      121 ccatctaatc gtataatact ttcattaatg acaagattat tcaaatcaaa attagcgtca
      181 tagggaaaat tagaaacgag caaataaaga atatctatca tcttttcttc tatttttaaa
      241 aatgattcaa tactatcaac ctcacgtttt accatggtgt atttattggt tgcttctttt
      301 attggatcgt caggtatagt gcttgttaaa aaatatcgtt tgtatcgctc ttcaagaatt
      361 gttttaaaat aggatgatgt tgatacctgg tttaccagca aaatgataat aagaatgata
      421 aagaaaataa tactcagtga accataaaaa aaagcgatat aaaaattctt tttggacttt
      481 aattttaaag atgttccaaa ttcatctttt aaaaaatcaa tagttttatg cttatttcga
      541 tgaatataat ttatcaaggt accaaatgca atgaaaaatt gcgattggag ttcggtttca
      601 ttagtttgat tgatcaatgc ttgcattact acagggattt gtaaattttg tgaaattaat
      661 gaacttaatc cagcaatggc tgcgccacca ccagatataa ttattctttg aatggtaata
      721 ttggcagatt tattaatttc tacatttaga aataaaacaa cttcatgaat taagttatta
      781 atctcttcct gcaattcatt gaataatgat ttaaactttt gttgagttat atttaatttt
      841 tttggtaatt caaaatggat attattttca attgatgaaa aatcaaaaga taaatatgag
      901 aatatttcct gcaatgctac gttttcaggt ttatattttt caaaatgctg tactaaattt
      961 tttactccaa acaaaagaca acgtgttgca attaattcat tattaatgac aaggttaatt
     1021 attgattttt cataaccaat attgagttgt actaccgatt cgtcctgtac agtgttgaaa
     1081 tacacatatg aatgataaag agcattatgt tcagaaccaa taaatgttat agcaagatta
     1141 tatgttgaaa ataggtcaat taacttattt attaaagatt tttgtgacac aactgcaata
     1201 acatccattc cattcgatgt ttttatttca acaggctgaa aatcatatat taccgattct
     1261 atatcaagag gtatacaatc agcaatttcg tagtgtataa cttcagatat ctgttcaata
     1321 ttattaaaag gaaagtttaa tgaataataa aataatttat tagatggcaa agttgttatt
     1381 attgtataat cagtaaaatc attatttgat atgattttat caattgcaat ttcaatccga
     1441 ttaaatttat cttctactga taaatccaat ttttcaataa tgagtgattc tatctgaaaa
     1501 tttttaaacc ctgtttttgc tttaatacat tttattgagc ttgaaccaat atcaaaaacg
     1561 gctaaatttt caaacaagct aaacctcccg tctcatgctt cggaccagaa tataatttgt
     1621 ttacgctctc tgtgataaaa tattatgatt gataatgtgg ttgagccaac tttccctatt
     1681 gatctaatct gaaaaatata tgatctcact gttaataaat tcttcctgac attttcatca
     1741 gtaattatat cactgatttc atcaacattt ttaaaaggtt tctgtaaacg ccttcttatt
     1801 aa
//
LOCUS       DAOSOX010000341         1780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_66606_length_1780_cov_8.227826, whole genome shotgun sequence.
ACCESSION   DAOSOX010000341 DAOSOX010000000
VERSION     DAOSOX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66606_length_1780_cov_8.227826"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..>1780)
                     /gene="lon"
                     /locus_tag="PLX22_13130"
     CDS             complement(231..>1780)
                     /gene="lon"
                     /locus_tag="PLX22_13130"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT20884.1"
                     /translation="LHLNHESREKIFEEINRLNYIESNTPEYTIARTYIDTVLSLPWD
                     TYTDDYLNIEYAEKILKKSHHGLNDVKERIIEFLSVRMLKPDSGGSILCLVGPPGVGK
                     TSLGQAIADSLKRNFFRVSLGGIRDEAEIKGHRRTYIGAMPGKIIQGMKICKSKNPVF
                     MLDEIDKLYQSYQGDPAASLLEVLDYEQNHAFRDHYIDMPFDLSKVFFITTANTSETI
                     PDVLLDRMEVIRIPGYIAEEKYEIAKHFLLPKQLKKHGLAHNPIKLDKNALYCIINGW
                     AREAGVRGLDRQIERLCRKKAKSIVKEDDSFPEHLDSNELRRLLGAEIYTDDTIPEKL
                     NPGVAIGLAWTTTGGSILLIESLSTPAQATPALTLTGNIGDVMKESATIAYNYIKHYC
                     DNNHQVIDYLTKNNIHIHVPAGAIPKDGPSAGITMATALYSLVLQQPITKRIAMTGEL
                     TLSGRILPVGGIKEKIIAAKREHCVAIILPAENKREIHEIPSHIIKDISLFFVAHIDE
                     VFDIVFH"
BASE COUNT          514 a          352 c          328 g          586 t
ORIGIN      
        1 cttcggatgt tactgttact ataccgccca tggcagtgcc attgatctgt gcactggcaa
       61 cacgggaaat acagagtatg attacaacca ttcctatact gtatttatat atacatccct
      121 ttgccatatt tttataccga tagttcatat ataaaatagt attatgcact gtgtgtgcat
      181 tggaaaatct atttgataaa aacagcctat gaagtcatcg ttacgattgg ttaatgaaat
      241 actatatcaa agacttcgtc aatatgggca acaaaaaaca gtgatatatc ctttataata
      301 tgtgaaggta tttcatgaat ttcccgtttg ttttctgctg gcagtatgat tgcaacacaa
      361 tgttcacgtt tagcagcaat aattttttct ttaatgccgc caaccggcaa tatccgccct
      421 gaaagggtaa gttcacctgt catagcaata cgttttgtaa ttggctgttg caataccagt
      481 gaatagagcg ctgttgccat ggtaataccg gcagatggtc catctttggg gatagctcct
      541 gcggggacat ggatatggat gttattttta gtaagataat ctattacctg atgattgtta
      601 tcacagtaat gtttaatgta attataggcg atagttgccg attctttcat gacatcgcca
      661 atattacctg tcagtgttaa tgcaggtgtt gcctgtgctg gcgtggaaag cgattcaatt
      721 aatagaatag aaccaccggt tgttgtccat gccaatccta ttgctacgcc tgggttaagt
      781 ttttcaggaa tggtgtcatc agtatatatc tccgctccca gaagcctgcg aagttcattg
      841 ctgtcaagat gttcagggaa tgaatcatcc tctttaacaa ttgattttgc cttttttcgg
      901 caaagacgtt caatctgtct gtctaaacct cgtactcctg cctcacgcgc ccaaccgttg
      961 atgatgcaat ataaagcatt tttatctaat tttatgggat tatgggctaa accatgtttt
     1021 ttaagctgtt ttggcaacaa aaaatgtttt gcaatttcat acttttcttc agcaatgtat
     1081 cccggaatac gaatgacttc catacgatca agtaacacat cagggatagt ttcagatgta
     1141 tttgcagttg taatgaagaa aacctttgaa aggtcaaaag gcatatcaat ataatgatcc
     1201 ctgaatgcat gattttgttc ataatcaaga acttctaata acgatgcagc agggtctccc
     1261 tggtagcttt gatacagctt gtcaatctca tccagcataa aaacaggatt tttagattta
     1321 catatcttca tgccctgaat tatcttcccg ggcattgctc caatataggt cctacggtgt
     1381 cctttaatct ctgcctcgtc gcggattccg cccagtgaaa ccctgaaaaa atttcgtttt
     1441 aaagaatcag caatagcctg tcccagggat gttttcccta cacctggcgg cccaacaaga
     1501 cacaatattg aaccacctga atcaggtttt agcatcctta cacttaaaaa ttcaataatc
     1561 ctttctttga catcatttaa tccatggtga cttttcttta aaattttttc tgcatactca
     1621 atatttaaat aatcatcagt ataggtatcc catggcaaag aaagaacagt atcgatatag
     1681 gtacgggcta tggtatattc cggagtattg gattcaatat aattaagcct gtttatttcc
     1741 tcaaaaatct tttctctgga ttcatggtta agatgcaatg
//
LOCUS       DAOSOX010000342         1771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_67088_length_1771_cov_6.606061, whole genome shotgun sequence.
ACCESSION   DAOSOX010000342 DAOSOX010000000
VERSION     DAOSOX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67088_length_1771_cov_6.606061"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1771
                     /gene="dnaE"
                     /locus_tag="PLX22_13135"
     CDS             <1..>1771
                     /gene="dnaE"
                     /locus_tag="PLX22_13135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT20885.1"
                     /translation="DHNLNEQNKVNKELITMAAQLNVGLIATNDAHYLNKEDAYYHDV
                     LLCIQTGKSLNDQKRMRFPNAEFYLKTPQQMETIFEDYPDALYNTVKIAEMIDCQIPL
                     GNAILPNFEVPEGYTRDSYLTELVNTGAQKRFGAIVPDHVKSRIEYELSVIQRMQFSG
                     YFLVVWDFIQFAKKHGISVGPGRGSAAGSMVSYCLEITELDPMKYNLLFERFLNPERN
                     EMPDMDIDFCALRRDEVIEYVKQKYGEDHVSQIITFNKMKSKGAIRDVARALEIPLQQ
                     VNALTKLIRHEDLEEALQASDELKQLYKTNDTGKQLIDIAKKVEGLTRSAGKHAAGVV
                     ISREPLMEHVPLYKDPKDGSISSQYEKESLEKAGLVKMDFLGLANLTIIDTCIKLIKK
                     HKNIAIDIAAIPLDDAKTFKLLQKADTMGVFQLESSGMQNILLKLGPTNFDDIIAIVA
                     LYRPGPLDSGMVEEFIERKRNPKKVEYLHPLLEPILKDTMGVIVYQEQVMLISQAMGG
                     FTLPDADKLRKAMSKKKMDIIDAMEDKFVQGAKQKKIDESVARTMYELMRTFGRYGFN
                     KSHSAAYALVSYQTAYLKAHYPLE"
BASE COUNT          569 a          337 c          385 g          480 t
ORIGIN      
        1 gaccataatc tgaacgaaca aaataaagtt aacaaagagc ttattacaat ggctgcccaa
       61 ctcaatgtag ggcttatagc caccaatgat gctcactatc tcaataaaga agatgcatac
      121 taccatgatg ttttgctctg catacaaact ggaaaaagcc tgaacgatca aaagcggatg
      181 cgtttcccca atgctgaatt ttatctaaaa actcctcagc agatggaaac catttttgag
      241 gattatcccg atgcactgta caacactgta aaaattgccg agatgatcga ctgccagata
      301 ccattgggga atgctatcct gccaaatttt gaggttccag aaggttacac cagagatagc
      361 tatcttaccg agcttgttaa tacaggggca caaaaaaggt ttggggcgat agttcctgat
      421 catgtgaaga gccgtattga atatgaacta tcggttatac agcgaatgca gttttccgga
      481 tacttccttg ttgtgtggga ttttattcag tttgcaaaga agcatggtat atcggttgga
      541 ccggggcgcg gttctgccgc gggttcaatg gtttcatatt gtctggagat taccgagctt
      601 gatcctatga aatacaacct gctctttgaa cgatttttaa atcccgagcg caatgaaatg
      661 cccgatatgg atattgactt ttgcgcgttg cgtcgtgacg aagtcataga gtatgtgaag
      721 caaaaatatg gtgaagacca tgtaagccag atcattactt ttaacaagat gaaatcaaaa
      781 ggtgctatcc gcgatgttgc tcgtgcgctt gagatcccat tgcagcaggt taatgctctt
      841 acaaaattga tacgtcatga ggatcttgaa gaggcattac aggcttcgga tgagctgaaa
      901 caattatata aaacaaatga taccggcaaa cagctcattg acattgcaaa gaaggtagaa
      961 gggcttacgc gctctgcagg aaaacatgca gcaggtgttg tcatttcacg agagccgttg
     1021 atggagcacg tgccattata taaagacccc aaagatggaa gcatttcatc acaatacgaa
     1081 aaggaaagtc tggaaaaggc tggactggta aagatggatt ttttaggatt agccaacctc
     1141 accattattg atacctgtat aaagcttatt aaaaaacata aaaacattgc tattgatata
     1201 gcagctatcc cgcttgatga tgcaaagaca tttaagcttt tacagaaagc cgatacaatg
     1261 ggcgtattcc agcttgaaag tagtggcatg caaaatattc tccttaaatt gggacccact
     1321 aactttgatg acatcattgc aattgttgca ctctatcgcc cgggacctct tgattcaggg
     1381 atggttgaag aatttattga acgcaagcgt aatcccaaaa aagtagaata tctgcatcct
     1441 ttactggaac caattttaaa ggataccatg ggtgttattg tttatcagga acaggtcatg
     1501 ctcatatcgc aggcaatggg aggctttact ttacccgatg ctgataaact gagaaaagcc
     1561 atgagcaaaa agaagatgga tatcattgat gcaatggagg ataaatttgt tcagggtgca
     1621 aagcaaaaaa agattgatga atcagttgcc cgtaccatgt acgaattgat gcgaacattt
     1681 ggacgctacg gatttaataa atcacattct gcagcttatg ctttagtttc atatcaaacc
     1741 gcatatctta aagcacacta tccattagaa t
//
LOCUS       DAOSOX010000343         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_67553_length_1762_cov_6.423550, whole genome shotgun sequence.
ACCESSION   DAOSOX010000343 DAOSOX010000000
VERSION     DAOSOX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67553_length_1762_cov_6.423550"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLX22_13140"
     CDS             complement(<1..307)
                     /locus_tag="PLX22_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20886.1"
                     /translation="MNSEILKQLDGYDNVINEREVLKRYGYDYRLDRGFVRKYLERLH
                     PRYITTKIIDIIEETPSTKTLRLDSVEKYLPPFQAGQYISVNVDIDGIITSRPYSISS
                     "
     gene            complement(317..>1762)
                     /locus_tag="PLX22_13145"
     CDS             complement(317..>1762)
                     /locus_tag="PLX22_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT20887.1"
                     /translation="AAATLAKKGVKTLLIEKHNIPGGCATSFCRGRFEFEVSLHQLSG
                     MGTPERPGPLRSILDSVGVLDTLEFVQMDKLYRVVVPGLIDITLPADKEGIVTALQAK
                     FPEEKDAIKKFFDFVWQFFMEVIGAFYLNDPDINQKKYPLYYKYALKDTQSVLDEYFT
                     NPILKAVITPYWTYMGLPPSKLCFSDMAALLFSYAEFKPYHIKGGSQMLSNSLLEKFY
                     SNGGEALFNTEVQKLLVKNNSVYGVKTSDGTEFQSKYVLSNASAISTFVDMLDDDKVS
                     GQVIEPYKGSSIGTSFFTIYCGLDCEYDKVGINEETNFICNTANPEYDYELSKVVDTD
                     QSSFIVTCYNVFDNAMVPEGTSQVVLVTMKYADAWLKVPPAQYYDEKHRCAEAMLKNA
                     EKVFPGLVSHIEEMEIATPITHMRYLNHPGGGAYGFDQYVKDSTLFISPKPPINGLYC
                     AGSWYGSAGYQPTLTSGHSAAMAIVKDIRNK"
BASE COUNT          529 a          371 c          293 g          569 t
ORIGIN      
        1 atgaggaaat gctatatggc cgtgaagtta tgattccatc aatatcaaca ttaacagata
       61 tgtactgtcc tgcctgaaat ggcggtaaat atttttccac tgaatccaaa cgcaatgttt
      121 tggttgacgg cgtttcttct atgatgtcaa taattttggt agtaatatac cgtggatgca
      181 accgctcaag atattttctt acaaaccctc gatccaatct ataatcataa ccataccgtt
      241 tgagcacctc tcgttcgttt ataacgttat cataaccatc taactgtttc agtatttcac
      301 tattcatcat cccctcctat ttgttacgta tatcttttac tattgccatt gctgctgaat
      361 gtcctgatgt aagggttggc tgatagcctg cagaaccgta ccagctccct gcacaataaa
      421 gaccattgat gggtggttta ggcgaaataa acaaagtaga atctttaaca tactgatcaa
      481 atccgtatgc tcctccaccg ggatgattta aatagcgcat atgggttata ggcgttgcta
      541 tttccatctc ttcgatatgg cttacaagtc cggggaaaac tttttcagca tttttaagca
      601 tcgcttccgc acagcggtgt ttttcatcat aatactgtgc aggtggaact ttaagccatg
      661 catcagcata tttcatggtt accagtacaa cctgactggt gccttcagga accattgcat
      721 tatcaaaaac attgtagcat gttacaataa atgaactctg gtcggtatcc acaacttttg
      781 ataattcgta atcatattct ggattggcag tgttgcatat aaaattggtt tcttcattga
      841 ttccaacctt atcgtattca caatcaagac cgcaatatat agtaaaaaac gatgttccaa
      901 tgctactacc tttatatggc tcaataacct gaccagaaac cttgtcgtca tccagcatat
      961 caacaaatgt actgattgct gatgcattgg ataaaacata ttttgattga aattcagtgc
     1021 catctgatgt ttttactcca taaacgctat tatttttaac aagaagcttc tgtacttctg
     1081 tattaaataa tgcttcaccg ccattgctgt aaaatttctc aagcaaacta tttgacagca
     1141 tctgcgagcc gccttttatg tgatatggtt taaactcagc ataagaaaac aacaacgctg
     1201 ccatatcact aaaacataat tttgaaggag gcaagcccat ataggtccag taaggagtta
     1261 tgactgcttt tagtattgga ttggtaaaat attcatctaa tactgattgt gtatccttta
     1321 aggcatactt ataatataat ggatattttt tttgatttat atccggatca tttagataaa
     1381 aagctcctat tacctccatg aaaaactgcc atacaaaatc aaaaaatttt ttaatggcat
     1441 ctttttcctc aggaaatttt gcctgtaatg cagtaacaat cccttcttta tctgctggca
     1501 gtgtaatatc aatcaaaccc ggtactacca cccggtaaag cttgtccatc tggacaaact
     1561 ctaatgtatc aagtacaccc acgctatcaa gaatgcttcg taatggacca ggcctttcag
     1621 gggtacccat tccactcaac tgatgaagtg aaacttcaaa ttcaaaacga cccctgcaaa
     1681 aactggtagc acaccctcca ggaatgttat gcttttcaat taaaagtgtt ttgacacctt
     1741 tttttgcaag tgttgctgct gc
//
LOCUS       DAOSOX010000344         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_68454_length_1746_cov_4.516263, whole genome shotgun sequence.
ACCESSION   DAOSOX010000344 DAOSOX010000000
VERSION     DAOSOX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68454_length_1746_cov_4.516263"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLX22_13150"
     CDS             <1..597
                     /locus_tag="PLX22_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOT20888.1"
                     /translation="RYDDFSDTAQNFFSTRQYTFSGYPGSFLQGLLESWKTEVTHTAR
                     KVVIGLPGIFHDTYLAKEKDACFFIAQHQYNTLSHRDDYSIIRDLMDSGRIQCTHACH
                     AVEQIHEVCDMLYNQGSMIVMLEGGAKLAGSFFNADAIDQCMYFYCPLIMGNGKGIIE
                     GKGRPYINESIHLYDVSTVYLGGDVLVSGYKEPYTCEA"
     gene            600..1241
                     /locus_tag="PLX22_13155"
     CDS             600..1241
                     /locus_tag="PLX22_13155"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOT20889.1"
                     /translation="MFTGIIEEVGTVTSMVQSANGKLISISAHTVLEGTKAGDSICID
                     GACQTVTQVGVDNFTVFASDVTLSITTLGMLKQGATVNLERAMQPQGRFGGHFVQGHV
                     DGTGTIVAVKKLPDGLSLMVDVPQSVSMFIVPKGSIAVDGISLTVVHKGNTIELYCIP
                     ETMANTTLSTKKRGDRVNLEVDIIAKYVYSMVQSIKGGDSSLETKLKDEGFWM"
     gene            complement(1304..>1746)
                     /locus_tag="PLX22_13160"
     CDS             complement(1304..>1746)
                     /locus_tag="PLX22_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20890.1"
                     /translation="AQLQGHTKQYSPQSLYEDYALAHCLTHSGIHVILEAGGYRDYFL
                     FELRIYGTGGAIVIGNGYLHVYTPEKSQFYTGFNDLVEQPVHPKGKPDYFVQLYKEAK
                     QCLKHPEQVTSSGIDGYRALEVIHAIYHSASQDGKTITLPLDCT"
BASE COUNT          483 a          333 c          412 g          518 t
ORIGIN      
        1 cgctatgatg acttttctga tactgctcaa aactttttta gcaccaggca atacacattc
       61 tcaggatatc ccggtagctt tctacaggga ttgctggaat catggaaaac tgaagtaacc
      121 catactgcca gaaaggttgt cattggactg cctggtattt tccatgatac ctatcttgct
      181 aaagaaaagg atgcctgttt ttttattgca cagcatcagt ataatacact atcgcataga
      241 gacgattact ccattatcag ggatttaatg gatagtggca ggatacagtg tacccatgca
      301 tgccatgctg ttgagcagat ccatgaggtt tgtgacatgc tatataatca gggctcaatg
      361 atagtaatgc ttgaaggtgg tgcaaagctt gcgggctctt tttttaatgc agatgctatt
      421 gaccagtgta tgtattttta ctgcccattg atcatgggca acggtaaggg cattattgaa
      481 ggcaaagggc gtccctatat taatgagtct atacacctgt atgatgtcag cacggtgtat
      541 ttaggaggtg atgtgcttgt ttcaggctac aaagaacctt atacatgtga ggcataataa
      601 tgtttaccgg tattattgaa gaagttggta ctgttacatc aatggtgcaa tcagcaaatg
      661 ggaaattaat ttccatcagt gctcataccg tgctggaggg aacaaaagcc ggtgattcca
      721 tttgcattga tggtgcctgc caaactgtta cacaggtggg tgttgataat tttacggtgt
      781 ttgcatccga tgttacgctc tccatcacaa cgcttggtat gctgaagcag ggggcaacgg
      841 ttaatctgga acgtgcaatg cagccgcagg gcaggtttgg cggacatttt gtgcaggggc
      901 atgtggatgg cacaggcacg attgttgcag taaaaaaatt gcctgatggg ctttcgctca
      961 tggttgatgt tccgcaaagt gttagcatgt ttattgtgcc taaaggttca attgctgttg
     1021 atggcatatc attaacggtg gtgcacaagg gtaacactat tgaattgtat tgcatcccgg
     1081 aaactatggc caatactacg ctttccacaa agaaacgcgg cgatagggtt aaccttgagg
     1141 ttgatatcat tgctaaatat gtttattcca tggtgcagtc aataaaaggc ggtgatagtt
     1201 cccttgaaac aaagctaaaa gatgagggat tctggatgta aagtttatga aaataaaacc
     1261 cgtgatatac cccggattaa aatcaaaacc atacattact gcttcaagta caatcaaggg
     1321 gaagcgtgat ggttttacca tcctgtgagg ctgaatggta gattgcatgg ataacctcta
     1381 atgctctata cccatctatg ccgcttgatg ttacctgttc aggatgttta aggcattgct
     1441 ttgcttcttt ataaagctgt acaaaataat cgggtttccc tttgggatgc acgggttgtt
     1501 caacaaggtc attgaagcct gtataaaact gtgatttctc tggtgtgtaa acatgcaaat
     1561 atccattccc aataactatc gcaccgcccg ttccgtatat ccgtaactca aacaaaaagt
     1621 aatcgcggta tccccctgcc tcaaggatga catgaatacc ggagtgtgtt aaacaatgcg
     1681 ccagtgcata gtcttcgtat aatgactgcg gcgaatattg tttggtatgt ccctgtagtt
     1741 gtgcga
//
LOCUS       DAOSOX010000345         1729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_69410_length_1729_cov_9.234767, whole genome shotgun sequence.
ACCESSION   DAOSOX010000345 DAOSOX010000000
VERSION     DAOSOX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69410_length_1729_cov_9.234767"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="PLX22_13165"
     CDS             <1..1168
                     /locus_tag="PLX22_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010699736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOT20891.1"
                     /translation="DDDNRLTKTVDTTSGSSVTTQYNYDAKGMRIIKDGPYGKSIYID
                     TGYVESGASQTTAPIASNHIFMGNTRVASVVKHTEEKQPSTYFYASDHLGSSSVLTTN
                     TGSYHERIEYLPYGETWVEDKAVSDGYTTPYKFTGKELDAETGLYYFGARYYDARMSR
                     WISADPALADGKYFPKPNDYDTEHDFYWYLQQDGSKKLAGLGGVFNAVNMDVYHYAGD
                     NPVKLVDPDGNDIILLHDAEGAIKRGHNAILIGDDKNGWDLFSKGGEKFIVSGKDSNQ
                     FLTFKTLEEFMDSEYSKRYEGKYRVRTSEKEDFEMQMAAIDEIGNEYSIFGRGEKTQD
                     GKKEINCADFVYEVIEKGTKGRINIDRPEHRLGYTDPKEQFKNFKEKNKGIEIE"
BASE COUNT          613 a          228 c          385 g          503 t
ORIGIN      
        1 ggatgacgat aatagactca caaagacggt ggacaccacc agcgggagca gtgtaacaac
       61 acagtacaat tacgatgcaa aaggcatgcg catcatcaag gatgggccgt atgggaagag
      121 catctatatt gacaccggct atgtagaaag cggggcatca caaacaacgg caccaatagc
      181 aagcaaccat atctttatgg gcaacacacg ggtggcaagc gtggtaaaac atactgaaga
      241 aaagcaacca tcaacctatt tctatgcaag cgaccacctt ggttcaagca gtgtgcttac
      301 caccaatacg ggaagctacc acgagcgcat tgagtatctg ccctatggtg aaacatgggt
      361 ggaggataaa gcagtcagtg atggctatac aacgccgtat aaattcaccg ggaaggaatt
      421 ggatgcggaa acaggcttgt attactttgg tgcacggtat tacgacgcga ggatgagtag
      481 atggataagt gccgacccgg cgcttgcgga cggtaaatat tttccgaaac ctaatgatta
      541 tgataccgaa catgattttt attggtactt acaacaggat ggcagcaaaa agttagcggg
      601 attgggtggt gtatttaatg cagtaaatat ggatgtgtat cattacgctg gggataatcc
      661 ggtgaaactg gttgatccgg atgggaatga tattatatta ttacatgatg ctgaaggtgc
      721 aataaaaaga ggacataatg ctatattaat tggagatgat aaaaatggtt gggacctatt
      781 ttcaaaaggc ggtgaaaaat ttatagtatc aggaaaagat agtaatcaat ttttgacatt
      841 taaaacatta gaagaattta tggatagcga gtattctaaa cgatatgaag gtaaatatag
      901 agttaggaca tcggaaaagg aagattttga aatgcaaatg gcagcaattg atgaaatagg
      961 caatgaatat tctattttcg gaagaggtga gaaaacacaa gatggtaaaa aagaaattaa
     1021 ttgtgctgat tttgtatatg aagttataga aaaaggaaca aaagggagga ttaacattga
     1081 tagacctgag catagattag gatacactga tccgaaagaa caatttaaaa attttaaaga
     1141 gaaaaataaa ggaatagaaa tagagtgaat ataaaatatt acaacctttt aatattttcg
     1201 ataatttatt ttattattgc aatgctttat gttcatgtag atgataatag cacttgcagt
     1261 atagattgta tatcattgat ttattttatt attatttcgt taaatagtat aacatattca
     1321 attataatat ttagaaacat acaaaatttt aaaattatag aattgttaaa aaattatttt
     1381 ttattgttat ttgtatctaa ttttactaat atttgtttga tgatagtata tgactttcct
     1441 gactgggggg ttgattatga aggtttagct gtaggtttta tatattttat tataagcata
     1501 ataatcacat ttatatttta cattacagga atatttcttg taaaaataaa aatgtatata
     1561 agccgtaaaa agaattcata cagtttcaat ggtaaatgag aatgtctgtt aggataaaat
     1621 agagagtgat ttctatgttt gagtgcgtgc ctttaatctg tggggtgtta ctgcaagcat
     1681 acggcagccg ggcaaggacg cccggcgcat tgggatgcgg gtgcatgat
//
LOCUS       DAOSOX010000346         1725 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_69670_length_1725_cov_5.365868, whole genome shotgun sequence.
ACCESSION   DAOSOX010000346 DAOSOX010000000
VERSION     DAOSOX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69670_length_1725_cov_5.365868"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="PLX22_13170"
     CDS             complement(<1..>1725)
                     /locus_tag="PLX22_13170"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HOT20892.1"
                     /translation="EYDVLSGNPWVDGDELWVEIPGIEYIVYKAIDPAEIAFGGGFRN
                     WYYGRWNADEYMYKANAPETYSYEFRPMDSSYIKIDAAEAINDPEGFAKKLIVCSSMT
                     PFYKEKVDDTTQSELNEVNPSTTNPLLPVWRGLDDDSWIGPGVFATTRVAKKNIEAII
                     NNSDEGVIDEGFLTQIHTMLASSTGIVDMRGVSKMTKSYNSTHSLGGPGSSVTITTCK
                     SYTKRQFFDINGDRYPDIITDYGLIYTGVNGAIKGWTMGIQGPVQKILSTNYNFGICP
                     GSNSSQVESEQKGSGKVEKPKQSFPSLSFSLATGDNRSTVDYQDINGDGLPDRIKHSG
                     MLYYAVQYNMGYSLSNDHIIENLYEIQHTKNKSSSLSVGYSTDKSKISGGLSILENRS
                     EKMDIPLLNDDDRSTLLDINGDGLPDRIVEYNNGLKIYVNTGDGFVEYNKKSIGIMDT
                     TKGRSWTLCFTIGEGIKILPPPLFIGTLIIDGGKSVTKDGNSTTYALRDINGDGLPDI
                     VYQSNNKLYARCNRTGKTNMLKGIKNSIGGSIQLDYQRAGNTHAMPQSQWVLAAVTAD
                     DGTGALDG"
BASE COUNT          429 a          419 c          290 g          587 t
ORIGIN      
        1 ccccatcaag tgcgccagtg ccgtcatcag ccgttaccgc tgcaagcacc cactgacttt
       61 gcggcatggc atgggtgttg cccgctcgct ggtaatcaag ctgtatgctc ccccctatgc
      121 tgtttttgat tcccttcagc atgttggttt tccccgtgcg attgcaccgt gcatatagct
      181 tgttgttgct ctgatacaca atgtcgggta atccgtctcc attgatgtcg cgaagtgcat
      241 aggtggtgga attgccgtct tttgttacac tttttccccc atctattatc aacgtcccaa
      301 taaaaagcgg aggaggtaat atttttattc cttccccaat agtaaaacat agtgtccacg
      361 atctcccttt tgtagtatcc ataatgccaa tgctcttttt attgtattca acaaatccat
      421 cacctgtatt aacatatatc ttcaatccat tattgtactc aacaatcctg tcaggcaatc
      481 catcaccgtt gatgtcaagc agtgtactcc tatcatcatc attaagaaga gggatatcca
      541 ttttttcgct tctattttcc agtatagata gtccaccact tatttttgat ttatctgtac
      601 tatatccaac actcaatgaa ctgctcttgt tttttgtatg ttgaatttca taaagatttt
      661 caataatatg atcattactt aaactgtatc ccatattata ctgtacagca taatataaca
      721 ttccactgtg cttaattctg tcgggcaaac catcaccatt gatatcctga taatcaacgg
      781 tgctacggtt gtctcctgtc gctaacgaaa aggaaaggct tgggaaggat tgcttgggtt
      841 tttcaacttt accggaccct ttttgttctg attccacctg gcttgaattt gatccagggc
      901 atataccaaa attatagttt gtgcttaata tcttttgtac cggaccctgt atgcccattg
      961 tccatccttt tatagcaccg ttaacgcccg tataaataag tccataatca gtaataatat
     1021 cagggtacct atcaccattg atatcaaaaa actgtcgttt ggtatagctt ttacatgttg
     1081 tgatagttac tgatgagcca ggtccgccta aactatgtgt agaattgtaa ctctttgtca
     1141 tctttgatac gcctcgcata tctacaatgc cggtactgct tgcaagcatg gtgtgtatct
     1201 gcgttaaaaa tccttcatca attacaccct catcgctgtt gtttataatc gcctctatat
     1261 ttttctttgc cacccgtgtg gttgcaaaca caccgggacc tatccagcta tcatcatcca
     1321 gtccacgcca taccggcaat agcggatttg tcgttgatgg gtttacctca ttcagttcac
     1381 tttgtgtggt atcatccact ttttctttgt aaaatggcgt cattgatgaa catactatca
     1441 atttttttgc aaacccttca ggatcattta ttgcttctgc tgcatctatt tttatataag
     1501 agctatccat gggtctaaat tcatagctgt atgtttcggg cgcatttgcc ttatacatat
     1561 actcatcagc attccatctc ccatagtacc agtttctgaa gccccctcca aatgctattt
     1621 ctgcaggatc aatggcttta tacactatat attctatgcc gggaatctcc acccatagct
     1681 catcaccatc cacccatgga ttgccgctca acacatcgta ttcca
//
LOCUS       DAOSOX010000347         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_69918_length_1720_cov_6.732733, whole genome shotgun sequence.
ACCESSION   DAOSOX010000347 DAOSOX010000000
VERSION     DAOSOX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69918_length_1720_cov_6.732733"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..1555)
                     /gene="hisS"
                     /locus_tag="PLX22_13175"
     CDS             complement(302..1555)
                     /gene="hisS"
                     /locus_tag="PLX22_13175"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT20893.1"
                     /translation="MIQKPPGVEDIFPDRITRFNYIINTAKEVFRLYNYREVILPIME
                     YTEVFARGIGDATDIVTKEMFTFEDRGGRSLTLRPEGTASMVRAYVENGEYNRLSLCK
                     FFYCGPMFRAERPQKGRLRQFNQFGAELFGSDHPYFDFEILSIMNDIATKLHIHDYEL
                     LLNSIGCPDCRKPYITELFNYYKNHEHELCDDCKKRLYTNPLRLLDCKVESCVAIKQD
                     APKITQFLCKACNEHFDNVQKLLQKHTIKYSLHPLLVRGLDYYTKTTFEFVTAQLGGQ
                     NAFAAGGRYNNLVELFGGKPTPAVGFAAGIERIELLLEQTMLNDALDVYIVYANETTV
                     SVVMHIANLLRKNNISCDFDPITKGFKTQFKRADREKAKFAIIVGQDELANNSVTIKD
                     MTSGEQVAILQSDVINFLSKKLTKP"
BASE COUNT          559 a          315 c          287 g          559 t
ORIGIN      
        1 tgaaagatag ttattcgaaa tatggtaggt actataaatt aagtaaagta ctttattaaa
       61 aaaatgaatt ttttatttag caacaaatgt ttctaatgaa tatatcaaac ataaaccaac
      121 ataaagcata tataataaaa cacaatagat agagctaaat agaaatagat agaacaagtg
      181 gatatttgaa agatgaaaat ttagacttta tgctgatgca ccatatttta aaaccacttt
      241 attgtgatat tattaatatt atggagcaaa gatgctgtaa aaagtggagt ttcaataata
      301 attatggttt tgttagtttt tttgatagaa agtttataac atctgattgc aagatagcta
      361 cctgttcacc agatgtcata tccttaatgg taacagagtt attggctaat tcatcctgac
      421 caactataat ggcaaatttt gccttttccc tatcagcacg tttaaactgc gttttaaatc
      481 ctttggtaat tgggtcaaaa tcacaggata tattgttttt ccttagaaga ttggcaatgt
      541 gcataactac cgatactgta gtttcattgg cataaacaat atatacatcc aaggcatcgt
      601 tgagcatcgt ttgctcaagc aacaattcaa tacgctcaat accggcagca aatcctacgg
      661 caggggttgg cttgccgcca aataattcga caaggttatt atatcgacca ccagcagcaa
      721 atgcattctg tccacctaat tgcgcggtaa caaattcaaa ggttgttttt gtataataat
      781 ctaaaccacg taccagaagt ggatgaaggc tatacttgat agtatgtttt tgtaaaagct
      841 tttgaacgtt atcaaagtgc tcgttacaag ctttgcataa aaattgtgtt atctttgggg
      901 catcctgttt tatggcaaca cagctttcaa ccttacaatc taataatcgt aatggattgg
      961 tatataaacg ttttttacaa tcatcacaaa gttcatgctc atggttttta taataattga
     1021 ataactcggt aatatatggt tttcggcagt caggacaacc aattgaattt aataaaagct
     1081 cataatcatg aatgtgtagt ttggtagcaa tatcgttcat aatggatagt atttcaaaat
     1141 caaaataagg atgatcacta ccaaataact ccgcaccaaa ctggttaaac tgtcgcaatc
     1201 ttcctttttg aggtctttct gctctgaaca taggtccaca ataaaaaaat ttgcacagtg
     1261 ataaacgatt atattcgcca ttttcaacat aggcacgaac catggaagct gtaccttcgg
     1321 gcctgagtgt taagcttcta ccacctctat cttcaaaggt aaacatctcc tttgtcacga
     1381 tatccgttgc atcaccaatc cctcttgcaa acacctcagt atactccata atgggtaata
     1441 tcacctcgcg gtaattatag agtcggaaaa cctcttttgc ggtattgatg atatagttaa
     1501 atctggtgat gcggtcagga aatatatcct ctacacctgg tggtttttga atcattgcgg
     1561 ttctccttat ccagtataat ctataatcgt ttgcgcacaa ttgcccatct gtcaacaggt
     1621 ttatcaaaca ctacggtagc ataggtgttt ggttgtgcca tttcacattt tttgccatca
     1681 acctcaatgt caataactat cgccccgcta tcccttatac
//
LOCUS       DAOSOX010000348         1685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_71972_length_1685_cov_6.885276, whole genome shotgun sequence.
ACCESSION   DAOSOX010000348 DAOSOX010000000
VERSION     DAOSOX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71972_length_1685_cov_6.885276"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLX22_13180"
     CDS             complement(<1..329)
                     /locus_tag="PLX22_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20894.1"
                     /translation="MKCDETKQLIEVNHVTIEGSKQSLHDVSFTLCLGEDVIFFGAEN
                     SGIDFICPLLAHAVDNYTGDVLYKGQDIKTMDFVEVHNFRKISGYLQRGYALISNMTV
                     YENIALPL"
     gene            complement(436..1233)
                     /locus_tag="PLX22_13185"
     CDS             complement(436..1233)
                     /locus_tag="PLX22_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT20895.1"
                     /translation="MLSVIKKNFFSIIVKINAMVNGFNDFQRYMKSIVLSFRSLRYLK
                     IRSLYTIVINQVKFTGIDALPLVSWIALLLGGTVIIQANTNFPKYGIESFIGNLLVII
                     IARELGPLVTALIVVARSGSAIAAEIATQVQNREIFSLELMGIDTRMYIILPRIVASF
                     TAIFTLIIIFDVIAFFGGYFIALTTVYIPVETFIQTLLDAFTFNDLLVTITKSIIYGI
                     LIPLICCYYGLKPRSSFEIPIYVSKAVIRTLLVIFIINAMISVLFYF"
     gene            1299..>1685
                     /locus_tag="PLX22_13190"
     CDS             1299..>1685
                     /locus_tag="PLX22_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20896.1"
                     /translation="MKKYLVISMAYMGKIVKLVIIVLCIILVILQWSRIETTVNLFKG
                     YFLQVKELLITPDTVSIIYPAKTLYYKRPYQKSTANIFHCGNLLFYKTTQEESVAAIA
                     NKIIGYTYYYKLYHLQNSIKNINALST"
BASE COUNT          585 a          336 c          273 g          491 t
ORIGIN      
        1 agcggcaggg caatattttc atacaccgtc atattgctta tcaaggcata cccccgttgt
       61 aaatagccag atatcttcct gaagttatga acctcaacaa aatccatggt tttaatatcc
      121 tgacctttat ataaaacatc accggtatag ttatcaacgg catgagctaa cagtgggcat
      181 ataaaatcaa tgccactgtt ttcggcacca aaaaaaatga catcttcacc aaggcatagt
      241 gtaaacgaca catcatggag gctctgtttg cttccttcta tggtgacatg gttcacttca
      301 atcagttgct ttgtttcatc acatttcatg gtattctcat caaacatagt gcaataaatt
      361 cttcaacgcc tgttgcatca ttcatctgca acccagctca ttaccataac tatcgaacaa
      421 aacaatttgt tgcttttaaa aataaaacaa caccgatatc atagcattaa taataaaaat
      481 gacaagcaat gtacggataa cagccttaga aacatatatg ggaatctcaa aacttgagcg
      541 tggcttcaaa ccataatagc aacatatcag cggaattaaa ataccataaa tgatgctctt
      601 ggtgatagtt accagtaaat cattgaaggt aaatgcatca agcagtgtct gaataaatgt
      661 ttcaaccgga atatacaccg tggtaagggc tatgaagtaa ccaccaaaaa atgctatgac
      721 atcaaatatg atgatgagcg taaagatagc cgtaaacgaa gcaacaatgc gcggtaaaat
      781 aatgtacatg cgcgtatcga tgcccataag ctccagagaa aatatttccc tgttttgcac
      841 ctgcgttgct atctcagccg caattgctga accagagcgg gcaacaacaa tcaacgccgt
      901 aaccagcggt cccagttcgc gcgctatgat gataacaagc aaattcccaa taaagctttc
      961 aataccatat ttagggaaat tggtgttagc ctgtataata accgttcctc ccaacaacag
     1021 cgctatccat gatacaagcg gcagagcatc aataccggta aattttacct gattgatgac
     1081 aatagtgtac aacgagcgta tctttagata ccgtaatgag cggaatgaca gcacaatact
     1141 tttcatatag cgttgaaaat cattaaaacc atttaccatt gcattaattt ttacgattat
     1201 actaaaaaaa ttttttttaa ttactgacag catggtttac attttttaag ttgaatttat
     1261 ttacacactt ttacattgtg atacatttgg ctgttaaaat gaaaaaatat cttgttatta
     1321 gtatggcata tatgggaaaa atcgtcaagc ttgttattat tgttttatgc attattcttg
     1381 tcatcctcca gtggagcagg attgaaacta cagtaaatct ttttaaaggt tactttttac
     1441 aggtaaaaga gttgcttatc acacccgata ccgtttcaat catataccct gcaaaaacat
     1501 tgtactataa acgtccgtat caaaaaagta cagcaaatat atttcattgc gggaatctgc
     1561 tcttttataa aaccactcag gaagaatcag tagctgcaat agcaaacaag attattgggt
     1621 atacctacta ttataagtta tatcatctac aaaacagtat aaaaaatatc aatgcactat
     1681 ctacc
//
LOCUS       DAOSOX010000349         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_72699_length_1672_cov_5.859617, whole genome shotgun sequence.
ACCESSION   DAOSOX010000349 DAOSOX010000000
VERSION     DAOSOX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72699_length_1672_cov_5.859617"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PLX22_13195"
     CDS             <1..1152
                     /locus_tag="PLX22_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOT20897.1"
                     /translation="MLCKNNWRLISKRYHVIDFYRSYYDFKQEQKFDQWQAIRKKLKF
                     TDPVISELNIKQPKTFVLIIGESLSRHHMQLYGYPRETNPKLTAMKDKLFVFNDVVSP
                     ATTTIDVMQLVLTLADHEHPEYYFEKRSIVNLFADAGYDTVWLGNQNFRGNRYNIGHG
                     IIASECKTAFELRGDNDGIVIDALSKILSSENNKDKIVFIHLRGSHTEYSKRYPKEYS
                     YFDNTIIPIPYDKGFSNNQKKIIDEYDNSVRYNDHIIATIIKLVKEQCEYSWVLYFSD
                     HGEEVFEYRDMFGHQRKNFSKYMCEIPFILWVSDKYKAANRELFAKLPGYLNRPYSTE
                     DVIHTISSLSKLKYADFDPSRSILSANFIVKDRFVNNKPYKSVPPQKIE"
BASE COUNT          566 a          267 c          304 g          535 t
ORIGIN      
        1 atgctatgta aaaataactg gcgacttata tccaaaaggt atcatgttat agatttctat
       61 cgctcatatt atgactttaa gcaggagcag aagtttgacc agtggcaagc aataagaaaa
      121 aagttaaagt ttactgatcc ggtaatctcg gaactgaata ttaaacaacc taaaactttt
      181 gtattgataa taggtgagtc attatcacgg catcatatgc agctttatgg atatccaaga
      241 gaaaccaatc ccaagctcac agcaatgaag gataagctct ttgtttttaa tgatgtggtt
      301 tccccagcaa caacaaccat tgatgtaatg caactggtat tgacactggc agatcatgag
      361 catcctgaat attattttga aaagcgatca attgttaatc tcttcgctga tgcagggtat
      421 gatactgtct ggttaggcaa ccagaatttc aggggtaaca ggtacaacat tggacatggt
      481 ataatagcaa gtgaatgtaa aacagctttt gagttaagag gcgataacga tgggatagtg
      541 atcgatgcac tgagtaaaat attgagtagt gaaaataata aagacaaaat agtgtttatt
      601 catctacgag gttcccatac agaatacagt aaacgatatc caaaagaata tagctatttt
      661 gacaacacga tcattccaat cccttatgat aaaggttttt ctaataatca aaaaaagatt
      721 atagatgaat atgataatag cgtacggtat aacgaccata tcattgcaac aatcatcaag
      781 cttgttaaag aacagtgtga atattcatgg gttctctatt tttctgatca tggggaagag
      841 gtttttgagt acagggatat gtttggtcat cagaggaaaa atttttcaaa atatatgtgc
      901 gagattcctt ttatactgtg ggtatctgat aagtataagg cagccaaccg tgaattattt
      961 gcaaaattgc ccggatacct caatagacca tattctactg aagatgtaat ccatactata
     1021 tcatcgttat ctaaattgaa atatgctgat tttgatccaa gcaggagtat actttctgct
     1081 aattttatag taaaagacag atttgttaat aataaacctt ataaatcagt tcctcctcag
     1141 aaaatagaat agtattgtgt cattctgagg gagcgttagc ctctgaagaa tccatacttt
     1201 catcaaatga taagactcta cacttcgttc agattgacat gccaccccat tgtcattccg
     1261 ggctagaccg ggaatctgct tttttgcgtt atgttccggt agtataatgg taactctaat
     1321 agtattttga caatgaaatc attcgttgct gcaaaaatac aacacgtatg aagtttgctt
     1381 ttcaacctat tttcatcaca tgattggtgt tattgctaaa ctatagatac cagcaagctg
     1441 gctaacggca gttcctttaa attaaaaaag atagattcaa ggaatgctac catcgattgc
     1501 tattacgatg acttcatctg gtatatatac tcttgtatgc ttcaattttt aacgtattgc
     1561 agttttttga tgtatgaata acaaaaaata aaaatatctt gacaattaat cgtattaacg
     1621 atggtgtgct aaaaacattc ttattatatg ttatatggtt gttatgactt tt
//
LOCUS       DAOSOX010000350         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_73826_length_1654_cov_5.525954, whole genome shotgun sequence.
ACCESSION   DAOSOX010000350 DAOSOX010000000
VERSION     DAOSOX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73826_length_1654_cov_5.525954"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLX22_13200"
     CDS             complement(<1..628)
                     /locus_tag="PLX22_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20898.1"
                     /translation="MSMISKQITIVCGSLIIVSMTVCSWAQDKNRVTFLYTNSLNGII
                     DACQCAADPKGGLVKRGYAVAQLKTKYPGAVLLESGDFCSYYPDTLLTKYIMKAYRHI
                     GYTAVGIGDQEFAAGVNTFHQYINELPFVSANLYYSIGGRWLSPKKNITVTVGTMKIG
                     ITAIIDNDAFKYYPNDIITKIKVTDITEALKKEIAAMEKMDFIVLLSHC"
     gene            complement(861..>1654)
                     /locus_tag="PLX22_13205"
     CDS             complement(861..>1654)
                     /locus_tag="PLX22_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOT20899.1"
                     /translation="KVYIDTAIFLKSHKLYPKDLDNIVLKLEYLYRYQQLKYAMTIYQ
                     KLINDMDYEKWSHVIQSIEYERDMVLQKILNAKQKVIELNNPMVKEIDVEKEVESLYM
                     MLHSLECYDIENKPMEIVTNDDIKNLKIKRGSLNEDERHEIESHVVHTYNFVSTIPWP
                     PEFSQIPEIAIKHHEKLDGTGYPYGLKGDAIPLQARIMALADIFDALIATDRPYKPPI
                     TFNQALKIIEEEACNNKLDKELVALFINKKIYDQFDRDAYRIKAQ"
BASE COUNT          464 a          348 c          281 g          561 t
ORIGIN      
        1 cgcaatgcga aagcagcaca ataaaatcca ttttttccat ggcggcaatc tcttttttca
       61 atgcttcggt tatatcggta accttaatct tggtgataat atcattgggg tagtacttaa
      121 aggcgtcgtt atcaataatg gcggtaatac ctattttcat ggtaccgaca gttactgtta
      181 tatttttttt tggcgacaac catctgccac cgatagaata gtacaggttg gcactcacaa
      241 acggcagctc attgatgtac tgatggaatg tgttaacccc tgcagcaaac tcctgatcgc
      301 caatccccac agccgtatag ccaatatgcc ggtaggcttt cataatatat tttgtaagca
      361 gggtgtcagg atagtaacta cagaaatcgc cactttcaag gagtactgcg ccaggatatt
      421 ttgtttttaa ttgggcaaca gcatacccgc gcttaacaag ccctccctta ggatcggcag
      481 cgcactgaca ggcatcgata atcccattaa gggagttggt atacaaaaat gttaccctgt
      541 ttttatcctg tgcccatgag catactgtca ttgatactat aattaaactg ccacatacaa
      601 ttgttatctg cttcgatatc atactcatag atatgcactc ctctataaaa aatagattta
      661 tcaacgattg ccattccggg cttgcccgga ccacctttgt cattctggac ctgttccaga
      721 atccagatat ataactttag atcccgactc ggcgttcagg atgactatcg gttttacctc
      781 atcataacat tgagcaccat tgttatactg aatcatgaca tgcatccagt acaggtttgc
      841 tctggaattt aaaaagcaaa ctattgcgct tttatcctgt aggcatccct atcaaattga
      901 tcatatattt ttttatttat aaacagagca accaattctt tgtcaagttt attgttacag
      961 gcttcttcct caattatttt taacgcctga ttaaaggtaa ttggtggttt gtatggtctg
     1021 tcggtagcaa ttaatgcatc aaagatatca gccagtgcca taatccgtgc ctgcaatggt
     1081 attgcatcgc cttttaatcc ataggggtac cccgttccat ccagcttttc atgatgcttt
     1141 atggctattt cgggtatttg actaaactcg ggaggccatg gtatcgtact tacaaagtta
     1201 taggtgtgaa caacatgact ttcaatttcg tgccgctcat cttcatttaa gctgccacgt
     1261 ttaattttaa gattctttat atcatcatta gtaactatct ccattggctt attttcaata
     1321 tcataacatt caagcgaatg taacatcata tacagtgatt ccacctcttt ttccacatct
     1381 atttctttaa ccataggatt gttaagctct ataacctttt gtttagcatt gagtattttt
     1441 tgcaacacca tgtcgcgttc atattcaata gattgaataa catgcgacca cttttcataa
     1501 tccatgtcgt ttattaattt ttggtagata gtcattgcat attttaactg ctggtatcta
     1561 tacagatact ccagcttaag cactatatta tctaaatctt ttggatataa tttatgtgat
     1621 tttaaaaaaa tagcagtatc aatatatacc ttgc
//
LOCUS       DAOSOX010000351         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_75083_length_1634_cov_6.563648, whole genome shotgun sequence.
ACCESSION   DAOSOX010000351 DAOSOX010000000
VERSION     DAOSOX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75083_length_1634_cov_6.563648"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLX22_13210"
     CDS             <1..185
                     /locus_tag="PLX22_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOT20900.1"
                     /translation="CTTDTNLGYVGNINYPNAAQMTSTADLINQDHLDVIGLPPDTFD
                     EMEFYAAIFDYIANYD"
     gene            426..1463
                     /locus_tag="PLX22_13215"
     CDS             426..1463
                     /locus_tag="PLX22_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20901.1"
                     /translation="MKKQYWYGMIVLFIFVGIAFIIFHKSDTVQSRIDYIKNGGVVPP
                     VNMLLKAFEADEELQARLHADFDMQDVIQYFRDKYGKTINHKYTQVMLIEKLRSYLMQ
                     MYPDDWVVYLYAMVREIFPGMENEILDTFEKLNKYDTWLKDNNGDLAKMDTKDRRDML
                     WKKRYEIFGQDAEDVWAAEKQTEPIYSALDTIKNSNDMTFNEKLTLYKDSIKEAYKNG
                     APDSVARKQQELMNTFLDVEGIQKDLAAMAPEERKENLKELRKAMGLDNAALQRWDEL
                     DQVRDQRWDAGMQYMTERTELEKHYTGSELDKRLDSLRMKYFGDEAATIKAEEDSGYY
                     RFKQQRKYGME"
BASE COUNT          552 a          241 c          372 g          469 t
ORIGIN      
        1 attgtacaac cgatactaat ttaggatatg ttggcaatat caattatcct aatgccgctc
       61 agatgacttc aactgccgat ttgataaatc aggatcacct ggatgttatt ggtttgccgc
      121 ctgatacatt tgatgaaatg gagttttatg cagcaatatt tgattatata gcaaactacg
      181 attaattgta tagtacagcg cactttcata aaaatccttc tccatacagt ggaggaggat
      241 ttttttagat gagggcaggt aaaaagtaag tgtggcataa tgctttggcg atagttactg
      301 ttgaatggta tgttgctgtc aaatactatt tgtagcatgg aagtataaaa acctggttaa
      361 atagtataca tgacatgatt cgcactaaaa caatgatacc atgattacat tatggggata
      421 aaagtatgaa aaaacaatat tggtatggga tgatagtatt attcatattt gtgggaatag
      481 cttttattat attccataaa agcgatacgg tgcaatcgcg tattgattat ataaagaatg
      541 gtggggtggt accaccggtg aatatgctgc taaaagcatt tgaagctgat gaagaattac
      601 aagcacggct ccatgctgat tttgatatgc aggatgttat acagtatttc agagataaat
      661 atgggaaaac aatcaaccat aagtatactc aggtaatgtt aattgaaaag ttaagaagtt
      721 atcttatgca gatgtatccg gatgattggg ttgtatatct gtatgctatg gtgcgtgaga
      781 tatttcctgg tatggaaaac gagatacttg atacatttga aaagttgaat aagtatgata
      841 cgtggctaaa ggacaacaat ggtgatctgg caaagatgga tacaaaggat agacgcgaca
      901 tgttgtggaa aaagcgatat gaaatttttg ggcaggatgc cgaagatgta tgggcagcag
      961 aaaaacaaac cgagcccatc tacagcgcac ttgacactat caagaatagc aacgacatga
     1021 catttaacga aaagttgact ttatataaag atagtattaa agaagcatat aaaaatggtg
     1081 ctcccgatag tgttgcccgc aaacagcagg agcttatgaa tacctttctt gatgttgaag
     1141 gtatacaaaa agatcttgca gctatggctc ccgaagagcg taaggaaaat ctaaaagagc
     1201 tacgcaaggc aatggggctt gataatgcag cattgcagcg ctgggatgaa ttggatcagg
     1261 ttagagatca gcgctgggat gccggtatgc agtatatgac cgagcgtaca gagctggaaa
     1321 agcactatac agggtcagag cttgacaaac ggcttgatag tttgcgcatg aaatattttg
     1381 gtgatgaagc agctacaata aaagctgaag aggacagcgg gtattatagg tttaaacagc
     1441 aaagaaaata tggaatggaa tagtaaaaat aggcttttat aaacaatgtt atccaagcaa
     1501 atcaagcagt tcatctttat tgagccgtgt aagccatgtt tcgccttcgg ttatcactaa
     1561 atcgttcagc tctcttttat gttgaatcat ggcattgatt ttttcttcaa atgaactttt
     1621 agtaacaaac ctgt
//
LOCUS       DAOSOX010000352         1618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_76099_length_1618_cov_8.884197, whole genome shotgun sequence.
ACCESSION   DAOSOX010000352 DAOSOX010000000
VERSION     DAOSOX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76099_length_1618_cov_8.884197"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLX22_13220"
     CDS             <1..1194
                     /locus_tag="PLX22_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT20902.1"
                     /translation="IASITNQPTFSLSGIWWQIIIFGSIGLVFSILLAKLLSKTLTMK
                     TSIIKEFLNSVGEGNLTAYTKKIAIADELADINIAVYDMKENLKAMVGLIFDSSRELK
                     TTSEDMKSSAIKFSEASRDLTAIIEETSSAYEEMSSSFEMIVNTIKSQVEQATAVKEN
                     IDSINQAGNNVSQQINALANTFNEAITGIEKGRETMQHAVAAITDISNYLQTVESTVS
                     AINEVADQINLLALNAAIEAARAGEHGRGFAVVADEVNKLADQTATLVKSIQSTIDQY
                     TGKVKNEIQFISHTANLFETVRDNIVNTGKVLTTTQEFTKNLNDQNANIQEKIQKLVQ
                     LSNEIYTTSNEQQITIDELTKAINAINEVASHTSDESVNIQDISEKLEENAAKLLKNI
                     EFFKL"
     gene            complement(1253..>1618)
                     /locus_tag="PLX22_13225"
     CDS             complement(1253..>1618)
                     /locus_tag="PLX22_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT20903.1"
                     /translation="TQKIMAVLILGFFVGIMTGIFGAGGGLMILLILIFVLNFPIHMA
                     IGTSSLLMTITACSGAFGYALHGNIRILPGLITGISAAGSGVAGAFFANKANEQILAM
                     VIGILYIMLGIVMTILSLW"
BASE COUNT          614 a          302 c          285 g          417 t
ORIGIN      
        1 atcgcttcca ttaccaatca gccaacattc agcctgtcag gaatctggtg gcaaatcatc
       61 atctttggaa gtattggact tgtgttttca atactgcttg caaaacttct gtcaaaaaca
      121 ctaacaatga aaacgtcaat tattaaggaa tttctaaact ccgttggaga aggcaattta
      181 acagcatata ctaaaaaaat tgctattgct gatgaattag ctgatatcaa tatagcagta
      241 tatgatatga aagaaaattt aaaagccatg gttgggctta tattcgatag ttctcgtgaa
      301 ttaaaaacca ctagcgagga tatgaaaagt tcagcaatca aattttcaga agcttcgcgg
      361 gatcttactg caatcattga agaaacaagt tctgcttatg aagagatgtc ttcctcattt
      421 gaaatgattg ttaacacgat aaaatcgcag gtagaacagg caactgctgt aaaagaaaat
      481 attgattcaa tcaaccaggc aggcaataat gtatcacaac agattaatgc actggcaaat
      541 acatttaacg aagctataac tggcatagaa aaaggaaggg aaactatgca acatgctgtt
      601 gcggcaataa ccgatatatc aaattacctg cagactgtag agtcaacggt ttccgccatt
      661 aacgaagtag ccgatcaaat taacctatta gcactcaatg cagcaattga agcagcccgc
      721 gcaggtgaac atggcagagg gtttgctgtt gttgcagatg aagttaataa attagctgat
      781 cagactgcta cacttgtaaa aagcatacaa tcaaccattg atcaatatac cggtaaggta
      841 aaaaatgaga tacaatttat cagccataca gccaatttgt ttgaaacagt acgtgataac
      901 attgtaaaca ccgggaaggt attgacaact acacaggaat ttacaaaaaa tcttaatgat
      961 caaaatgcaa acattcagga gaaaatacaa aagcttgtac aactttcaaa tgaaatctac
     1021 acaacatcaa atgaacagca aattacaata gatgagctaa caaaggcaat taatgcaatc
     1081 aatgaagttg catcacacac ttccgatgaa tctgtaaaca tccaggatat atctgaaaaa
     1141 ttagaagaaa atgctgctaa gctattaaaa aatatagaat tttttaaact ttgatgagga
     1201 atgagataga cacatactat cgcccaaata atggtagcct tccagagtac aattaccata
     1261 acgataaaat ggtcattact ataccaagca taatgtatag tattccaatc accatagcca
     1321 gtatctgctc gtttgcctta ttagcaaaaa aagcgcctgc tacaccactg cctgcagcac
     1381 taatacctgt tattaatcca ggaagaattc ttatattacc atggagggcg tatccaaatg
     1441 cacctgaaca ggcagttatt gtcatgagaa gtgatgaagt accaattgcc atatgaatag
     1501 ggaaattgag aacaaaaatc aggatgagca gtatcataag tcctccacca gcgccaaaaa
     1561 ttccagtcat gatacccaca aaaaagccca atatcaaaac agccatgatt ttttgagt
//
LOCUS       DAOSOX010000353         1608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_76707_length_1608_cov_4.474565, whole genome shotgun sequence.
ACCESSION   DAOSOX010000353 DAOSOX010000000
VERSION     DAOSOX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76707_length_1608_cov_4.474565"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLX22_13230"
     CDS             complement(<1..247)
                     /locus_tag="PLX22_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOT20904.1"
                     /translation="MFIDSHAHFDLCAEDHKTSAETIISLMEENNVTTAVHIVIDTTG
                     IQWGLDFINRHTDKNIYLAIGIHPSSLADSEALHQLSV"
     gene            449..577
                     /locus_tag="PLX22_13235"
     CDS             449..577
                     /locus_tag="PLX22_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20905.1"
                     /translation="MTVILPPIRDENDKNLTLNNAHFTLRRRGDEGTVNNFIFRSV"
     gene            580..972
                     /locus_tag="PLX22_13240"
     CDS             580..972
                     /locus_tag="PLX22_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20906.1"
                     /translation="MDYILLSKTQIYGKRVFILFFLFVLFISFIQCSSFMSKDAQIGL
                     KKYEGEYIILTDASRGEYTIPKGERVRIKVIATEDYIKIYAMSAKSDIVKGEWILILY
                     LFPEDFEKEKFNQKVFEDKLYQVLKPVK"
     gene            1134..>1608
                     /locus_tag="PLX22_13245"
     CDS             1134..>1608
                     /locus_tag="PLX22_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT20907.1"
                     /translation="MNISFIIAMIIIGAITGMVTGITGASGVMIFVPLVSMVLKFSIH
                     EAIGTSLMVNVISSLAITYIYYKHGNINLKSGIWIAIGSIIGAQVGTGFSVKIPAISL
                     GAAFGIFMAGMGVTMWKKGINRESIAKTTKKILNLETQTQKIMAVLILGFFVGIMT"
BASE COUNT          533 a          233 c          319 g          523 t
ORIGIN      
        1 ttactgataa ttgatgcaat gcttcagaat cagctaacga tgatggatgt attcctatcg
       61 caaggtagat atttttatcg gtatgcctgt taataaaatc cagcccccac tgtatccctg
      121 ttgtgtctat gacaatatgg acagcagttg taacattatt ctcttccatt aaagagataa
      181 ttgtttcagc cgaagtttta tgatcttcag cacaaagatc aaagtgtgca tgggagtcaa
      241 tgaacatata taacctcgta aaataaaatt gagatacatt gtaatgtaat tgatttcagg
      301 tgcaatataa ttttttcata aaagcatact atgagtgtgc actatttaat gccataggta
      361 aaatataatt gacattaaaa gcacaggtgc cttgtattaa aagcagcact atttgaatta
      421 tggagtatta atgtggtggg ggcaaccaat gacagtaata ttaccaccca tacgggatga
      481 aaatgataaa aacctcaccc tgaacaatgc tcacttcacc ttgcggaggc gaggcgatga
      541 gggtacagtt aataatttta tttttaggag cgtttaataa tggattatat acttttgtca
      601 aaaacacaaa tttatgggaa acgtgttttt atattatttt tcttatttgt actttttata
      661 tcatttatac agtgttccag ttttatgtca aaagatgctc aaataggttt aaaaaagtat
      721 gaaggtgaat atatcatatt aacggatgct tcccgtggtg aatatactat accaaaaggg
      781 gaaagggtac gaataaaagt tatagcaaca gaggattata taaaaatcta tgccatgtcg
      841 gctaaatccg atattgttaa aggtgagtgg atacttattt tgtatctttt cccagaagat
      901 tttgaaaaag aaaaatttaa tcaaaaagtt tttgaagata aactttacca ggtactaaag
      961 cctgtgaaat gatgtagcag ggtatattat gttgttttct gctcctgtag cattgaaatt
     1021 gaagaatcta tggtttataa ctactgacag gttttttcct ttttagtgtt aaacaatacc
     1081 attgattgga tattgattgg attgggaatt gtaacagtgg atgaggcaca tcaatgaata
     1141 tcagttttat cattgccatg attataattg gtgcaatcac cgggatggta accggtataa
     1201 ccggtgcttc aggagtaatg atatttgtac ctctggtgag catggtgctt aaattttcaa
     1261 tccatgaggc aataggaaca agtttaatgg ttaatgtcat atcatcgcta gcaataacat
     1321 acatctatta taaacatggt aatataaatt taaaatcagg aatatggata gccataggtt
     1381 caatcattgg ggcccaggtt ggtactggct tttcggtcaa aattcctgca ataagccttg
     1441 gtgcagcgtt tggaattttt atggcaggta tgggtgttac aatgtggaaa aaagggatca
     1501 atcgcgaatc gattgccaag acaacaaaga aaatactaaa ccttgaaacc caaactcaaa
     1561 aaatcatggc tgttttgata ttgggctttt ttgtgggtat catgactg
//
LOCUS       DAOSOX010000354         1596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_77531_length_1596_cov_9.311486, whole genome shotgun sequence.
ACCESSION   DAOSOX010000354 DAOSOX010000000
VERSION     DAOSOX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77531_length_1596_cov_9.311486"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLX22_13250"
     CDS             complement(<1..614)
                     /locus_tag="PLX22_13250"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOT20908.1"
                     /translation="MKNIITRTIKHINVVFSNKGYVLIIVLIIIAAIISISSEFLTLA
                     QTNTKYLQSFTNRQKAYWVARSGINLAIELLEADKKGSVANFISGDIETDPNIDSYKD
                     IWALPLPEIPLENGNIQIHIEDENSKINLSVLANEFVEQTPYYIITQRFCINMGLPLD
                     LADCILDWVDIDDARSPYGAESSDYYQNLKKPYKAKNDALDSIN"
     gene            complement(620..1294)
                     /locus_tag="PLX22_13255"
     CDS             complement(620..1294)
                     /locus_tag="PLX22_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT20909.1"
                     /translation="MKFHYKIRTTSDGFTLIEILVATAVSSIILLMVYTAHHSIIQAV
                     NSLSKTALFYEHVNLALYRMNSDFSCAYFDRYKEKLFFIGNNEYGDKSLGKVDFVSIN
                     TAEQLIAGKISESIPTSDVIEIGYFLKQMETPDEEGNYLYYLMRRSDITYDDDTTNGG
                     DSFVLLSNVVDCKFEFGVAQKYTDKWDSKEYRRFPNSVKTTLILKNPYGNDEKFIIIT
                     YINLSK"
     gene            complement(1296..>1596)
                     /locus_tag="PLX22_13260"
     CDS             complement(1296..>1596)
                     /locus_tag="PLX22_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20910.1"
                     /translation="IMSAVSSGILGITKNKNLTTAMLIAQSQMNKFLIDNQRGPDLQD
                     ESISEYPGFSFSRTITRYENAMIGPIDAYKVVITVKWLERNQERNHSISYIYQNR"
BASE COUNT          516 a          268 c          235 g          577 t
ORIGIN      
        1 tcattaatac tatcaagtgc atcattttta gccttatatg gctttttcaa gttttggtaa
       61 tagtctgaag actccgcacc atacggtgac cgtgcatcat caatatctac ccaatccagg
      121 atacaatcag ctaaatcaag tggtaacccc atattaatac aaaatcgttg tgtaataata
      181 taataaggag tttgttcaac aaattcattg gcaaggacac tcagatttat tttagaattt
      241 tcatcttcaa tgtgtatttg aatattacca ttttccagtg gaatttcagg gagcggtaat
      301 gcccagatat ccttatatga atcaatgttg gggtcagtct ctatatcacc acttataaaa
      361 tttgctacgg aacctttttt atccgcttcc agcaactcaa tagctaaatt gattcctgaa
      421 cgtgcaaccc agtatgcctt ttgtctatta gtaaatgatt gtaaatattt agtatttgtt
      481 tgtgctaacg ttaaaaattc tgacgaaata gatataatgg ctgctattat aataagcaca
      541 attattaata catatccctt attggaaaat actacattaa tatgtttgat agttctggtt
      601 attatatttt tcatgtttac tatttactta agttaatata agtaattatt ataaattttt
      661 catcattgcc ataaggattt ttcagtatta aggttgtctt tacagagttt gggaaacgcc
      721 ggtattcttt agaatcccac ttatcagtat atttttgagc aacaccaaat tcaaatttac
      781 agtcgacaac atttgataaa agtacaaaac tatcgccacc attagtagta tcgtcatcat
      841 aggtgatatc agaacgtctc attaaatagt ataagtaatt accttcttca tcaggagttt
      901 ccatttgttt taaaaaatag ccaatttcaa taacatcact ggttggtatt gattcagaaa
      961 tttttcctgc tattaattgt tctgcagtat ttatgcttac aaaatcaact tttccaaggc
     1021 ttttatcacc atattcatta tttcctataa aaaacaattt ttctttatag cggtcaaaat
     1081 atgcacatga aaaatcactg ttcatacggt aaagagccag attaacatgt tcatagaaca
     1141 aagcggtttt tgataagcta tttactgctt gaatgataga atgatgagca gtataaacca
     1201 taagcagtat aattgaagaa acggctgttg ccaccagaat ttcaataagt gtaaacccat
     1261 cagatgttgt gcgtatttta taatgaaact tcatattacc gattttgata aatataggat
     1321 atgctatgat tacgttcctg gtttctttcc agccatttaa ccgtaatgac tactttataa
     1381 gcatctattg gaccaatcat tgcattttca tatcgggtga tagttcgtga aaaactaaat
     1441 cctggatatt ctgatattga ttcatcctgc aaatcaggtc ctctttgatt atctattaaa
     1501 aatttattca tctgactttg agcaataagc attgcagttg ttaaattttt gtttttggta
     1561 attcctaaga ttcctgatga tacagcactc ataatc
//
LOCUS       DAOSOX010000355         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_77746_length_1593_cov_8.910923, whole genome shotgun sequence.
ACCESSION   DAOSOX010000355 DAOSOX010000000
VERSION     DAOSOX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77746_length_1593_cov_8.910923"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            555..977
                     /locus_tag="PLX22_13265"
     CDS             555..977
                     /locus_tag="PLX22_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20911.1"
                     /translation="MKTFTFSSDTVKLTKSKELIDNTATYELLFALSENAQCVELSVS
                     TQDRNGRKVRCAYIVESLVDVSKYAKKPAKPIYAEIAKNFDINWRFQSKISICNDKTD
                     PLKRLNKGNYRLRISAFTNDFYIFTVDITSPVEILFIQ"
     gene            991..1374
                     /locus_tag="PLX22_13270"
     CDS             991..1374
                     /locus_tag="PLX22_13270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT20912.1"
                     /translation="MPKHYDDIESEDLYEMAMQWAAQGFFEKAIELIKRSIELNPHFI
                     GAYIDLANLYAKKHQYQDAFHILKKASKIDTKFHRLNYLMAKYAYKSGNYIAALQYVD
                     KAIAISPEALYVKAKHIIAHKLSNE"
BASE COUNT          547 a          263 c          274 g          509 t
ORIGIN      
        1 tgcatcctat ggtaatactg aaagttacaa tggtttattt atctataata taaaagatag
       61 aaaaatagaa aaaattatca ctaccgaaga tgttattgcg ttgtgtgttc cgcgctaaat
      121 tttttatttc atgtacaggt aaaacgcttc tatccataag aatgaaaatg tggtatcatg
      181 tcatcctgaa ccgtagaacg tcatcctgaa ccccgattca ggatctatct aatagtatat
      241 attgagattc cgggtcaagc ccggaatgac agtgtggtaa agagattccg gaacgggaac
      301 ggaatgacat tgtcgccata attttattga tggtaaaagt tttccctctc gaatattagt
      361 ttactaaatt aaaaaatagc ttgaacatat gtttaatcca attcattatt atactcatgg
      421 gtttattaat aatcatttta ctttttattc caggatgaac aatgaaacgt agtaaaatgt
      481 tatactgttg tttgttgata catgtaccag tatttgtatc ctgtttatcc gtaccgtcat
      541 cacagcatga taaaatgaaa acatttacct tctcatctga cacggtaaaa cttacgaaaa
      601 gcaaagaact cattgacaat actgcaacct atgagttgtt gtttgcctta tccgaaaatg
      661 ctcaatgtgt ggagctatcg gtttccacac aggatagaaa cggcaggaag gtgcgctgtg
      721 cttatatcgt ggaatcattg gttgatgttt ctaaatatgc aaaaaaacct gctaagccca
      781 tctatgctga aattgcaaaa aattttgata tcaactggcg tttccagagt aagatatcaa
      841 tttgcaacga taaaactgat ccattgaaaa gactcaataa gggtaactat cgcctcagaa
      901 tttcagcatt taccaatgat ttttatattt ttaccgttga tataacatca ccggttgaaa
      961 ttttatttat acaataacta tcgcacaaat atgccaaaac actatgatga catagaatcc
     1021 gaagacctgt atgaaatggc catgcaatgg gctgcgcagg gtttttttga aaaagctatt
     1081 gagcttataa aacggtcaat agaattaaat ccacatttta ttggtgcata cattgatctg
     1141 gcaaaccttt atgcaaaaaa gcatcaatac caggatgctt tccatatatt aaagaaggca
     1201 tcaaaaattg atacaaaatt tcatagattg aattatttaa tggcaaaata cgcttataaa
     1261 agtggcaatt acatagctgc actacaatac gtggataaag ctatagcaat ctccccggaa
     1321 gcattgtatg tcaaagctaa acacattatt gcacataagc ttagcaacga ataacataat
     1381 agcgttcaat agccgcgacg gacaatgaac gtttttttat tatagaaagc aaaaccattg
     1441 actataaaat attttacttg atttaaataa agaatcttca cattattggt tttatatcat
     1501 tataattctc taaggaggtt tgtatgaaaa ggtttttatt agtggtactc atagtattta
     1561 ctgcttcaac ggtttttgct gcgggtattg gtg
//
LOCUS       DAOSOX010000356         1579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_78738_length_1579_cov_7.706693, whole genome shotgun sequence.
ACCESSION   DAOSOX010000356 DAOSOX010000000
VERSION     DAOSOX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78738_length_1579_cov_7.706693"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLX22_13275"
     CDS             complement(<1..833)
                     /locus_tag="PLX22_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT20913.1"
                     /translation="MRYYIIKRILIIIPTFIGITFITYLMIRLAPGDFTSLRAGIEGE
                     LKAGSLSKEIFEQEKKLYGLDKPIIVGYSEWLYKTVKLDFGTSRKDGRTVAAHIIESL
                     PITLSLNILSIVIVYIISIPLGIFSAIKKDTVIDRIVSITLFLLYSLPSFWVALLLLK
                     YLSGGDYLNLFPLGGLYSDWFDQLNVLQKAMDVIWHLILPVVTLTYGGFAFLSRYTRA
                     TMLEVINQQYIVTARAKGLSEKKVLFVHAFRNSLIPLITLMATMLPGLLGGSVVVESI
                     FS"
     gene            complement(833..>1579)
                     /locus_tag="PLX22_13280"
     CDS             complement(833..>1579)
                     /locus_tag="PLX22_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT20914.1"
                     /translation="YIGWNAKNPLFNNKNVRKALTMLINRQAILDSLLFGLGTVVTGP
                     FYIYDKYYNHDIQPLPYDTEKAKELLNSAGWVDSDGDGILDKGGTKFSFTFTIASASK
                     FAERLATILKEDFAKVGIQMDINRYEWAVFVNKIDKRDFDAVTLGWSLSWEGDPYQLW
                     HSSEVKAGSNFCYFINREADEIIEKARKEFDVNKRIALYRRFHEIINDEQPYTFLYCT
                     PALVAVSKRFTNVIVHKRGLNYVEWKVTGN"
BASE COUNT          508 a          340 c          268 g          463 t
ORIGIN      
        1 gagaatatag attcaaccac tacactgcca cctaaaagtc ccggcagcat tgttgccatc
       61 aacgtaataa gcggaattaa agaatttctg aaggcatgca caaataaaac ctttttttcg
      121 gacagccctt ttgcacgtgc tgttactata tattgctgat ttattacctc aagcattgtt
      181 gcacgggtat accgtgaaag gaaggcaaat ccaccatagg tcagggtaac caccggtaaa
      241 atcaaatgcc atataacatc cattgctttt tgcaatacat ttaattgatc aaaccagtca
      301 gagtataagc ctccaagcgg gaaaaggttc aaataatcgc caccgcttaa atattttaaa
      361 agaagcaatg caacccagaa ggacggcaga gaatatagta aaaatagtgt aatgctgaca
      421 atgcgatcaa tcacagtatc tttttttatt gctgaaaaaa tacctaatgg aattgaaata
      481 atataaacaa taactatcga caaaatatta agagataatg taataggcaa tgactcaata
      541 atatgtgctg ctactgtacg tccatctttt cgagaggtgc caaaatcaag ttttactgtt
      601 ttataaagcc attctgaata tccaactata ataggtttgt ccaatccata cagtttcttt
      661 tcctgttcaa atatctcctt ggacaggctc cccgccttaa gctctccttc tattcccgca
      721 cgtaatgatg taaagtcacc cggtgccaac cgtatcataa gataggtgat gaaggtaata
      781 ccaataaagg ttggtataat aattagtata cgtttgatga tatagtatct cattaattgc
      841 ctgtaacctt ccactctaca tagtttaacc cacgtttatg aacaataaca ttggtaaagc
      901 gcttgctcac cgcaacaagc gccggcgtac agtataaaaa ggtatagggc tgctcatcat
      961 tgattatttc atgaaaacgg cggtatagtg caatgcgctt gtttacatca aactcttttc
     1021 gagccttctc aattatctca tcagcttctc gatttatgaa ataacaaaaa tttgaacctg
     1081 ctttaacctc cgaagaatgc cacagttgat acggatcgcc ctcccatgat aatgaccacc
     1141 ccaatgtaac agcatcaaaa tcgcgtttgt caatcttatt gacaaacaca gcccattcat
     1201 atcggttgat atccatttga atgccaacct ttgcaaaatc ctcttttaaa attgttgcaa
     1261 gccgttcagc aaacttactt gctgatgcta ttgtaaaagt aaacgaaaac tttgtgccac
     1321 ctttatccag tatgccatcg ccatcactat ccacccagcc tgcactgtta agcaattctt
     1381 ttgccttttc cgtatcatag ggcagtggtt gtatatcatg gttgtaatac ttatcataga
     1441 tgtaaaatgg accggtaaca accgtaccca gcccaaaaag taagctatcc agtatagcct
     1501 gcctattaat gagcatggtg agagctttgc gaacattttt attgttaaac aggggatttt
     1561 ttgcattcca gccaatata
//
LOCUS       DAOSOX010000357         1558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_80206_length_1558_cov_7.130406, whole genome shotgun sequence.
ACCESSION   DAOSOX010000357 DAOSOX010000000
VERSION     DAOSOX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80206_length_1558_cov_7.130406"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLX22_13285"
     CDS             <1..538
                     /locus_tag="PLX22_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT20915.1"
                     /translation="LCKGCPHGFVFEVLKKLDIVVNGDIGCYTLAVLPPYSAMHSQGC
                     MGASIGMHSGFEKARGDEMARNSVAVIGDSTFVHSGITPLIDLVYNKGTGTVIILDNR
                     VTAMTGHQDHPATGKTLTGEDTHELDLELLCKAVGVKRVRKLDPKNMDEFEKAVKEEI
                     AAKEPSVIISLRKCILKK"
     gene            549..1124
                     /locus_tag="PLX22_13290"
     CDS             549..1124
                     /locus_tag="PLX22_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT20916.1"
                     /translation="MKSVIFAGVGGQGVILAGKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCHVCFGEKVYSPLIEKGTADFVIALELLEAMRKLEYLSTNGTLIVNKFKVNPAP
                     VEAGLAKYPDDIESWLCSNIGKCHVVDTAKVLKEAGSNKAVNIVMVGILSNFLEFDQK
                     AWEEAIRQSVKEKFLDMNIKAFNLGRKIPLS"
     gene            1207..>1558
                     /locus_tag="PLX22_13295"
     CDS             1207..>1558
                     /locus_tag="PLX22_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20917.1"
                     /translation="MMRYYTLTVFICLCFIVTCSMGNPKQLHENLPSMKAVNKVGILV
                     RIPASSPVEYTRYVTTLQSMLAGYKQIKEIVIIDSDNVAITTFQSNDDRFYQTSLDGD
                     FLYYKATGIVTSYCT"
BASE COUNT          500 a          205 c          371 g          482 t
ORIGIN      
        1 attatgtaaa ggatgtcctc atggttttgt atttgaggtg ctgaaaaaat tagatattgt
       61 tgttaatggc gatatcggtt gttatacatt ggcagtattg ccgccatatt ctgcgatgca
      121 ctcacaggga tgtatgggtg ccagcattgg tatgcattca ggatttgaga aagcccgcgg
      181 tgatgagatg gcacgaaata gtgttgctgt cattggtgat tcaacctttg tgcattcggg
      241 tattacacca ttaattgatt tagtatataa taaaggaact ggtacggtta ttatactgga
      301 taatagggta actgcaatga ccgggcatca agatcatcct gcaacaggga aaacacttac
      361 gggagaggat acacacgagc tggatcttga acttttgtgc aaagctgttg gggtaaagcg
      421 ggttagaaaa cttgatccaa aaaatatgga cgagtttgaa aaagctgtaa aagaagaaat
      481 tgcagctaaa gaaccttcgg ttattatttc attgcggaaa tgtattttga aaaaataggg
      541 gaatatttat gaaaagtgtc atttttgcgg gagttggtgg tcagggggtt atccttgcgg
      601 gtaagatatt aatgcaggtt gccatgaatg caggatatga tgttaaggaa tctgaagtac
      661 atggcatggc ccagcgaggc ggtagtgttg attgtcatgt atgttttggg gaaaaggtgt
      721 attcaccatt gattgaaaaa ggtacagccg attttgttat agcgctggaa ttactggaag
      781 caatgaggaa attagaatat ttatcaacaa atgggacgct tattgtaaat aaatttaagg
      841 ttaatcctgc gccagttgag gcaggtttag caaagtatcc tgatgatatt gagagctggt
      901 tatgtagtaa tattgggaaa tgccatgttg tggatacagc taaggtatta aaggaagcag
      961 gatcaaacaa agcggtgaat atagtgatgg tagggatttt atctaatttt ttggagtttg
     1021 atcagaaggc ttgggaagaa gctatacggc agagtgttaa agagaagttt ttggacatga
     1081 atattaaagc atttaattta ggaagaaaaa ttccattatc atgaactatc gccaaaaaaa
     1141 tttttttaaa acccgggttt aaaattatag atttggcaat agtaatcgtt ataaacaaaa
     1201 ggagttatga tgaggtacta tacattaact gtttttattt gtttatgttt cattgttacc
     1261 tgcagtatgg gaaatcctaa acagctccat gaaaatttgc ccagtatgaa agctgtaaat
     1321 aaggttggga tattggtacg aatacctgca agcagtcctg ttgagtatac acgatatgtt
     1381 acaacattac agtccatgct tgctggatat aaacaaataa aagagatagt tattattgat
     1441 agcgataacg ttgcaattac cacgtttcaa tccaatgatg accgatttta tcaaacttca
     1501 ttagatggtg attttttata ctataaagca acaggtattg ttacttcgta ttgcacca
//
LOCUS       DAOSOX010000358         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_80500_length_1554_cov_6.569046, whole genome shotgun sequence.
ACCESSION   DAOSOX010000358 DAOSOX010000000
VERSION     DAOSOX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80500_length_1554_cov_6.569046"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLX22_13300"
     CDS             complement(<1..225)
                     /locus_tag="PLX22_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20918.1"
                     /translation="MRVYDDTLYFNHGTLYNMRQTIVIIAIIALIGTISLFAQTVKDS
                     TPVDKPDNTKTPDVTANEQSTVTSKSKTISN"
     gene            419..>1554
                     /locus_tag="PLX22_13305"
     CDS             419..>1554
                     /locus_tag="PLX22_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOT20919.1"
                     /translation="MKIRSLIIALLGVVLLTQFVFADEKYALIELNGSVNPVVADYIV
                     NSIKKANQDNMQFVVMVMDTPGGLLNAMRDIIKAILDSQIPVIVYTYPKGAQAASAGG
                     FIMISAHIAAMSPGTEIGAMHPVSPFLNFSQDQKSDGIMEKKVLNDTVAYARSLAQLR
                     NRNIAWVEKAVREAISSTNNEALRLGVIDIVANDIDDLLKKIDNKSVKIKDKFIRLHT
                     HNIQQVQYTMNWKERVLNYFADPQMVFFLFIIAVVGIGFELKNPGMIVPGAIGAVAFF
                     LFLLAVKVLPINFAGLTLIILAIVLFILELKFTSYGLLTLGGIISFILGSMILFDSPL
                     PGGQIPLTSVFTVLIVLLLFIFIVVRAVIKVHTKKAVTGIEGMLG"
BASE COUNT          475 a          224 c          312 g          543 t
ORIGIN      
        1 attactgatt gttttgcttt tactggtaac tgtagattgt tcgtttgccg taacatctgg
       61 tgtttttgtg ttgtcaggtt tgtcaacagg agtagagtcc ttaactgttt gggcaaaaag
      121 ggagatcgtt cctattaatg caatgatagc aataatgaca atagtttgcc tcatattgta
      181 taatgttcca tggttaaaat ataatgtgtc atcgtaaact cgcattgtta atcatcatgt
      241 gggtatacga ttgataaagc tttttcaata tgttactgtt tgtcatataa aaaaaattaa
      301 gatatctcaa taataactat cggttatact attgcaattc tttttattta ttatcttgac
      361 tgttgcataa aatattgtat actgtatact attacaataa attattgagg ttttaaaaat
      421 gaagatacga tcgcttatca ttgcattgct tggtgtggtg ctattgacac aatttgtttt
      481 tgcagatgaa aagtatgcac ttattgaact gaatggttcg gtaaatccag ttgtagcgga
      541 ttatatagtt aattcaataa aaaaagccaa tcaggataat atgcaatttg ttgtaatggt
      601 aatggacacg ccgggtggac tcttaaatgc catgcgtgat attataaaag caatattaga
      661 ttcacagata cctgttattg tttataccta tcctaaagga gcacaggctg cctcggcagg
      721 tggttttatc atgatatctg ctcacatagc agcgatgtcg ccaggcactg aaattggtgc
      781 catgcatcca gtcagtccgt ttttaaactt cagtcaggat caaaaatctg atggaattat
      841 ggaaaaaaag gttcttaacg atactgttgc ctatgccagg agtttagcac aattacggaa
      901 ccgtaatatt gcatgggtag aaaaagcagt acgggaagca atttccagta ccaataatga
      961 agcgttacgg ctgggagtga tagatattgt agctaatgat attgatgatc ttttgaaaaa
     1021 aattgataac aaatcagtaa aaataaaaga taaatttata aggttgcata cacacaatat
     1081 acaacaggtt caatatacca tgaactggaa ggaaagagtc cttaattact ttgccgatcc
     1141 ccagatggtt tttttcctgt ttattattgc tgtggttggt attggatttg aattaaaaaa
     1201 ccctggtatg atagttcctg gtgccattgg agctgttgca ttctttttat ttttattagc
     1261 agttaaagtg ttgcccataa attttgctgg actaacacta attatattag ctattgtatt
     1321 atttatactg gaattaaaat ttacaagtta tgggttactg acattggggg gtattatctc
     1381 gtttatattg ggctcaatga tactgtttga ttcaccttta ccagggggac agataccgtt
     1441 aacatcggtt tttactgtac tcattgtttt actgttgttt atctttattg ttgtgcgtgc
     1501 tgtaataaag gtacatacaa aaaaagcggt gaccggaatt gaaggtatgc tggg
//
LOCUS       DAOSOX010000359         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_81116_length_1546_cov_7.330651, whole genome shotgun sequence.
ACCESSION   DAOSOX010000359 DAOSOX010000000
VERSION     DAOSOX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81116_length_1546_cov_7.330651"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLX22_13310"
     CDS             <1..336
                     /locus_tag="PLX22_13310"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOT20920.1"
                     /translation="AAVDIIESIQKLNKQREKEGAVTITVGIGIHMGNVVHGRMGSKD
                     RMDFTSIGDAVNLSARLCSHADAQTILVSEAIIINLSKKKFKGKKLGPIKVKGKEQPI
                     PIFQITGKA"
     gene            complement(337..726)
                     /locus_tag="PLX22_13315"
     CDS             complement(337..726)
                     /locus_tag="PLX22_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20921.1"
                     /translation="MQKVDILHRYLSEHSVPELIEKKLDANSFLTNNFAYHALRIGNS
                     IGDNLDISIEIIILDEIAKKYNLLLNTTEHAELHTQGIAESDLDNLVHAAILFENIKN
                     NKKLYKEILRKISYFIRKEFYPIIHQE"
     gene            complement(813..>1546)
                     /locus_tag="PLX22_13320"
     CDS             complement(813..>1546)
                     /locus_tag="PLX22_13320"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOT20922.1"
                     /translation="FYPRSPYGVAKLFAYWITINYREAYGMFACNGILFNHESSRRGE
                     TFVTRKITRGVARILFGLDKKIYLGNLNAKRDFGHAQDYVKGMYLILQQPKPDDYVLA
                     TGITTEIREFIALAFDEVGLTVTFQGEGIKECGIVISCHEDVLLSRLQSLHCAPEHKE
                     NILQTARACLNKVVVGIDQRYFRPSEVDILIGDPTKAREKLGWEPRHTVKDIVSDMII
                     GDLEKNYREYVLKTSGFTMSRSCGI"
BASE COUNT          512 a          315 c          238 g          481 t
ORIGIN      
        1 gcagcagttg atatcataga atcaattcaa aaattaaaca aacagcgtga aaaagaagga
       61 gcggtaacaa ttactgttgg gataggcatc catatgggga atgttgttca tggcagaatg
      121 ggttcaaagg acaggatgga ttttacatca attggtgatg ccgttaatct atccgcacgg
      181 ctgtgctcac atgccgacgc tcagacaata cttgtatctg aagcaattat aataaatctt
      241 tcaaagaaga aatttaaagg gaagaagctt ggtcccatta aggttaaagg taaagaacaa
      301 ccaataccca tcttccagat caccgggaaa gcataattat tcttgatgaa taattggata
      361 aaactctttc cgtataaaat agcttatctt acgcaatatc tctttgtata gttttttatt
      421 atttttaatg ttttcaaaca atattgctgc atgaacaaga ttatcaagat ccgattcagc
      481 aataccctga gtatgaagtt cagcatgctc tgtagtattt aataacaaat tatatttctt
      541 agctatctca tccagtatta tgatctctat ggaaatatca agattgtcac caattgaatt
      601 cccaatacgc aaagcatgat aggcgaaatt gtttgttaaa aaagaattag catccaattt
      661 tttttcaatc agctcaggca cactatgctc agataaatac ctatgtaata tatcaacttt
      721 ctgcatactc ccccctcact atgatagttg tttatatcct ctcaatctat aaatcattat
      781 gccagaagcg atgtaaaaat acaacaaaat aatcaaattc cacaacttct ggacatagta
      841 aaaccacttg ttttaagcac atattcacga tagttctttt caagatcacc tattatcata
      901 tctgaaacaa tatctttaac agtatgccgg ggttcccacc ccaatttttc acgcgccttt
      961 gttggatcac cgatgagtat atccacttca gaaggtctga agtaccgctg gtcaatgcca
     1021 acaacaacct tattgaggca agcccttgca gtttgcagta tgttttcttt atgctcaggc
     1081 gcacaatgta acgattgtaa tcgtgataac aatacatctt catggcaaga aataacaata
     1141 ccacactctt ttatcccttc gccttgaaat gtaacagtta atcccacttc atcaaacgcc
     1201 aatgcaataa attcccttat ctcagttgtt ataccggtag ctaatacata atcatcaggt
     1261 ttgggctgtt gcaatattaa atacattcct ttgacataat cctgtgcatg tccaaaatct
     1321 cgttttgcat ttagattacc taaataaatc tttttatcca gtccaaataa tatgcgagca
     1381 acccctcgtg taatttttcg cgtaacaaac gtttcccctc ttctggaact ttcatgatta
     1441 aataaaatac cattacaggc aaacattcca tatgcttccc tgtagtttat ggttatccaa
     1501 taagcaaaaa gctttgcaac accataagga cttcgtggat aaaatg
//
LOCUS       DAOSOX010000360         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_81558_length_1540_cov_8.175084, whole genome shotgun sequence.
ACCESSION   DAOSOX010000360 DAOSOX010000000
VERSION     DAOSOX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81558_length_1540_cov_8.175084"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLX22_13325"
     CDS             <1..1307
                     /locus_tag="PLX22_13325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT20923.1"
                     /translation="NSILVTGDIEQFAFEKASGNNCYTNNYRTYDNLARWSVYGSRVV
                     KEHPQFPTLIAEIETYRGCPRQQHCSFCAEGLFSFVDFRREEDIIAEIDTLIQQGITR
                     FRIGRQADIISYKAQLHHITNGFPRPHPQPIISLFHELQKRKESGKISMLNVDNANPG
                     SIYYYKNEASAIIDVLAQSVTAGDTLALGVESFDPAVIKKNNLKLQKDQLIEVIQLIN
                     DIGGTRVNDIHRLLPGINLIHGLIGEHADTFKINYESLQEILHKGLLVKRINIRSLVP
                     FPGTIAAQKQKKESKLLINRYRYYRQKIRHEIDHTMITKLYPAGTVIANMFVLSQVQG
                     YSYAKEIRSYAITAKIPLELTLYSFLDCIVVGHQERSLLCLPYPVNINTLPQKALATI
                     PGIGKKTASAMIVQRPFNDFAQAMPYLVSVPGKLIDAIKLCF"
     gene            complement(1304..>1540)
                     /locus_tag="PLX22_13330"
     CDS             complement(1304..>1540)
                     /locus_tag="PLX22_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20924.1"
                     /translation="SLKRRDLFGGFIGGMVIGVVGALIGGFILDKFLLDITIKILRFL
                     VYDTGVNIIAAFIGGYVAVYIMNKLNHNKERKRY"
BASE COUNT          496 a          347 c          292 g          405 t
ORIGIN      
        1 ccaattccat actggttacc ggcgacattg aacaatttgc atttgaaaaa gcatccggca
       61 acaattgtta taccaataac tatcgcacct atgataacct tgccagatgg tcggtgtacg
      121 gcagcagggt ggtgaaggaa catccacaat tccccaccct tatcgctgag atagaaacct
      181 accggggatg cccgcgacaa cagcattgct cattttgtgc cgaggggttg ttttcatttg
      241 ttgacttcag gcgggaagaa gatataatag ctgaaattga tacgctgata caacagggta
      301 ttacccgttt tagaattgga cggcaggctg atatcatttc gtataaagca cagctacacc
      361 acattaccaa tggattccca agaccacatc cccagcctat catttccctt ttccatgaat
      421 tacagaaacg aaaagaaagc ggaaaaatca gcatgcttaa cgttgacaat gcaaatcccg
      481 gtagcatcta ttactataaa aacgaagcat cagccatcat tgacgtactg gcacaatcgg
      541 tgactgccgg cgatacgctt gccctgggag tagaatcatt tgacccggct gttatcaaaa
      601 agaataatct aaaattgcag aaagatcagt tgattgaggt aatacagctt atcaacgata
      661 ttggcggtac tcgtgttaat gatatacaca ggcttctccc cggcatcaat ctcatccatg
      721 gacttattgg tgagcatgct gatacattta agataaatta tgaatcctta caggaaatac
      781 tgcacaaagg attacttgtt aaacgtatta atatacgaag ccttgtacca tttccaggaa
      841 ctatcgccgc acaaaagcaa aaaaaagaaa gcaaactatt gataaaccgt taccggtatt
      901 atcgccaaaa aatacgacat gagattgacc ataccatgat tacaaagctt taccctgctg
      961 gcacggttat agcaaatatg tttgtgcttt cacaggtgca gggttattcg tatgcaaaag
     1021 agattagaag ctacgccatc actgccaaaa ttccactgga gcttaccctc tatagctttc
     1081 tggattgtat tgtggttggt catcaggagc gttcattatt atgcctgcct tatcctgtga
     1141 atatcaacac acttccccaa aaagctcttg caacaatacc cggaataggg aagaagactg
     1201 catcagcaat gatagtacaa cgcccattta acgattttgc tcaggctatg ccgtatctgg
     1261 tgtctgtgcc ggggaaatta attgatgcaa taaaactgtg tttttaatag cgtttccgtt
     1321 ctttattatg attgagctta ttcataatat aaacagcaac ataaccacca ataaatgctg
     1381 caataatatt aacacccgta tcatacacaa ggaagcgcaa tatctttatg gtaatatcca
     1441 gtaaaaattt atccagaata aatccaccta tgagcgctcc aacaacccct atgaccatac
     1501 cgccaataaa accgccaaag aggtcacgcc gcttcagact
//
LOCUS       DAOSOX010000361         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_82076_length_1533_cov_4.481732, whole genome shotgun sequence.
ACCESSION   DAOSOX010000361 DAOSOX010000000
VERSION     DAOSOX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82076_length_1533_cov_4.481732"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PLX22_13335"
     CDS             <1..767
                     /locus_tag="PLX22_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT20925.1"
                     /translation="YFTHNEVIKNILNYFKLTQDEVFQRIVRYMQNMSLMLFSNLTNI
                     LTFSIRFFINFFFMLLILFFLFKDNERFEKSLYTVSPFPEDIERDMVDTLKIVVRILL
                     AGNFFIMMLQGIMVGIGFAIVGLATPVLWGSIAAIFSLIPVIGTSFVWLPASFYLLAI
                     GSTGKAVIVGLWCLGWYLLLENIVKPIVFGEKLNFHPLILFFLLIGSLQTFGLPGIII
                     GPILLTLFFSFWEMYKILDSYTIEQDKKIKKPHKNS"
     gene            936..1334
                     /locus_tag="PLX22_13340"
     CDS             936..1334
                     /locus_tag="PLX22_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20926.1"
                     /translation="MKIWVSSYTFDEVIAQKNAIDAAQNPAQGYMLLKELEKKRVIIQ
                     KAVVKEVVPSTNIDYNFCVVATVEHPSGSIECNIHTRNWRNEEDIDSIAKLKPGDAIR
                     VVGVFKRFLKLLGQQYVVEIVDANISKVKQ"
     gene            1341..>1533
                     /locus_tag="PLX22_13345"
     CDS             1341..>1533
                     /locus_tag="PLX22_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20927.1"
                     /translation="MGKLWAHCYAPKWDENDINLNLHAFPYIRIGNNAPLALRRRGAG
                     VRFNDFTFQEDGLLWSRTFS"
BASE COUNT          450 a          240 c          327 g          516 t
ORIGIN      
        1 aatattttac ccataatgaa gtcattaaaa acatacttaa ctattttaag cttacccagg
       61 atgaagtttt tcagcgaatc gtcaggtata tgcagaatat gtcgctcatg cttttttcca
      121 atctcaccaa tattttaaca ttttccatac gtttttttat caatttcttt tttatgctgc
      181 ttatattgtt ttttcttttt aaggataacg agcgctttga aaaaagccta tacaccgtgt
      241 cgccattccc tgaagatatt gagcgtgata tggtagatac actgaaaatt gtggtgcgca
      301 tcctgctggc aggaaacttt tttattatga tgttacaggg gattatggtt gggattggat
      361 ttgctattgt tgggcttgca acgccagtat tatggggaag tattgctgca atcttttcgc
      421 tgattcctgt aattggaaca agctttgtat ggctgcctgc ttcattctat ctgcttgcaa
      481 ttggtagcac tggtaaagct gtgatcgttg gactatggtg tttaggctgg tatttgctac
      541 ttgagaatat tgtaaagcct attgtttttg gtgaaaagtt gaattttcat ccactcattt
      601 tattcttttt attgataggc agtttgcaaa catttggttt accgggcatt atcattggac
      661 ccatacttct tacccttttc ttctcgtttt gggagatgta taaaatactg gatagctaca
      721 ccatagagca ggataaaaaa ataaaaaaac cacataaaaa tagttagggc gatagttact
      781 cataatttat tgtatttgta taaagatatt tgacaaaaaa atagtagtgg tgctatcctt
      841 gacaacctgt tgctacaaca attcccgagg taacgataat ggatattaaa aaatgtatgc
      901 tgatatttgt ttgttttttt ataggctgta cttctatgaa aatatgggtg tcgtcatata
      961 cgtttgatga agtgattgca caaaaaaatg caattgatgc cgcccagaac ccggcacagg
     1021 gctatatgct tttgaaggag cttgaaaaga agcgtgtcat tatacaaaaa gcggtggtga
     1081 aagaggttgt accttcaaca aacatagatt ataatttttg tgttgttgct acggtggagc
     1141 acccgtcagg aagcattgag tgcaatatcc ataccagaaa ttggcgaaat gaagaggata
     1201 ttgacagtat tgcaaagtta aaaccaggtg atgctatccg tgtagttggc gtttttaaac
     1261 gttttttaaa attgttagga cagcaatacg ttgtggagat agtagatgct aacatttcaa
     1321 aagtgaagca ataaaataat atgggtaaac tttgggcgca ttgttatgcg cccaaatggg
     1381 atgaaaatga tataaacctc aaccttcatg cctttcctta tataaggata ggaaataatg
     1441 ctcccctcgc cttgcgaagg agaggggccg gggtgaggtt taatgatttt acttttcagg
     1501 aggatggatt gttatggagt cgtacattca gcg
//
LOCUS       DAOSOX010000362         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_82389_length_1529_cov_5.056988, whole genome shotgun sequence.
ACCESSION   DAOSOX010000362 DAOSOX010000000
VERSION     DAOSOX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82389_length_1529_cov_5.056988"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1471
                     /locus_tag="PLX22_13350"
     CDS             350..1471
                     /locus_tag="PLX22_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT20928.1"
                     /translation="MSYAFELGPIRPPSEAYSILIRITRNCTWNKCAFCHTYKGQTFS
                     RRAIEDVLNDIDSIYTIAQRIGEVAGNTITQQTLQKAKGNDDTPLYYYEQVAFWMAYG
                     LHSVFLQDANSLVLSTRDITTILHHITSRFPAVERITTYARASTVSKKSLEDLIAIRQ
                     AGLSRLHIGMESGSDPVLQLVQKGTTQAQLIDAGQKAMQAGFDVSYYYMPGLGGKEYM
                     EENAIQSAAVINTVNPTFVRLRSTVPIPGTPLYDMMQKGQWTPLSEVQKVLEIRTFIE
                     HCNGITSTLQSDHIMNLLEDVEGTFPHDTAAMLSTIDKFLAMEKEDQDKFIIGRRTGM
                     YRYLSDYYPNFKIDQLYYAIVQHYQNVDTAMLEILKDYV"
BASE COUNT          473 a          321 c          288 g          447 t
ORIGIN      
        1 ctatctcacc ggtagcatag cctttcccaa tttcagtaca atatattcca ttatgttcta
       61 taaaagttcc ctttgcaaac tcatcccatg ataacgttat gccattctct aaagcaatag
      121 tttccaattt tgtaccctgc atgtaatacc cccatcagat gaaagtagtt gtgatatact
      181 atatatgagg tacagtacag tattatcaag caaaatagta ccacctctat ttatttgcta
      241 tgtgtacaat agctattgta ttggaggata ctgcaggtat aattgaatag ctaattttac
      301 ttaaaaatat ttatttttat ggtataatta tcattgagag gttataacta tgtcctatgc
      361 tttcgaatta ggtcccattc gtccgccatc tgaggcgtac agtatactaa tccgcataac
      421 ccgcaactgt acgtggaata agtgtgcgtt ctgccatacc tataaaggac aaaccttttc
      481 tcgcagagcc attgaagatg tactcaacga cattgattct atctatacca ttgcacagcg
      541 tattggtgaa gttgccggta acaccatcac acagcaaaca ttgcaaaaag caaaaggaaa
      601 tgatgataca ccgttgtatt actatgaaca ggttgcattc tggatggctt atggcttaca
      661 ttcggttttt ttacaggacg caaattcact ggtgctatcg accagggata taacaaccat
      721 ccttcaccat attaccagcc gttttcctgc tgttgaacgc attactacct atgcacgtgc
      781 cagtactgtc agtaaaaagt cgcttgaaga tttaatagcc atacggcagg cagggctatc
      841 ccgacttcac atcgggatgg aatcaggttc tgaccccgta cttcaactgg tacaaaaagg
      901 gaccactcag gcacagctca tcgatgcagg acaaaaagcc atgcaggcag gctttgatgt
      961 atcgtactat tacatgccag ggctgggtgg caaagaatac atggaagaaa atgccatcca
     1021 gagcgcggca gttattaaca cggtcaatcc aacatttgta cgtctgcgca gcaccgttcc
     1081 cataccggga actccactat acgacatgat gcaaaaaggt cagtggacac cactatctga
     1141 ggttcagaag gttttggaga tacgcacatt tatagagcat tgcaatggca tcaccagtac
     1201 cttgcaaagt gatcacataa tgaatctgct tgaagatgtt gaagggactt tccctcatga
     1261 tacagcagca atgctttcca cgattgataa gtttcttgca atggaaaaag aggatcagga
     1321 taaatttatt attggaagaa gaactggcat gtatcgctat ctatctgatt actaccccaa
     1381 ttttaagatt gatcagcttt actatgccat agtacaacac tatcaaaatg ttgatactgc
     1441 aatgctggag atactcaaag attatgtata gtttttttat attttgaatc aaattcattc
     1501 atttcatttg ttataaaagc aacagaatc
//
LOCUS       DAOSOX010000363         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_82540_length_1527_cov_6.382473, whole genome shotgun sequence.
ACCESSION   DAOSOX010000363 DAOSOX010000000
VERSION     DAOSOX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82540_length_1527_cov_6.382473"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLX22_13355"
     CDS             complement(<1..318)
                     /locus_tag="PLX22_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HOT20929.1"
                     /translation="MGLAVKIYEAFKDDETKARVLADVIESLEQIIKVNEVATRSDIK
                     NTELALTKEIEQIRKDMKETELKLTKEIENVRADLSKEIEKVRADLSKEIEKVRADLS
                     KEIE"
     gene            588..1061
                     /locus_tag="PLX22_13360"
     CDS             588..1061
                     /locus_tag="PLX22_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOT20930.1"
                     /translation="MNITSNSFASGGIIPVQYAMKGIPGGQNISPHIAIHDVPKEARS
                     LAVTFVDRHPMAHNWVHWLVLNIPANTKEIQEGASLKAMPHDSIEMINTFGFAGYGGP
                     QPPKGSGVHSYELCCYALAELFKIPKKELTENELLKLIKPLLITQCTIHGNFENK"
     gene            1176..>1527
                     /locus_tag="PLX22_13365"
     CDS             1176..>1527
                     /locus_tag="PLX22_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20931.1"
                     /translation="MGIPVYQYDTIIEVQKEGVCDSSEINALAAIEAQLKNDEKIRSL
                     LISETCIKNRTAALAGDIVRDMNHSNHIDIMMVLTGSFMFTADLARALYAQGGINTSI
                     HCIKTSVYDETIKSS"
BASE COUNT          485 a          313 c          249 g          480 t
ORIGIN      
        1 ctctatctcc tttgataaat ccgctcgtac cttctctatc tcctttgata aatccgctcg
       61 taccttctct atctcctttg ataaatccgc tcgtacattc tctatctcct ttgtcaattt
      121 caattccgtt tctttcatat ccttgcggat ttgctcaatc tctttcgtaa gagccagctc
      181 ggtgtttttt atgtcagagc gagttgcgac ttcattgacc ttaataatct gttctaatga
      241 ttcaataaca tcagccagaa cccttgcctt tgtttcatca tccttaaacg cctcataaat
      301 tttaactgct aaacccatta tatatccccc tatgcatata caaacatacc ttaaacccca
      361 caggtatcaa tatagttaca ttgctaacaa tgtcaaataa aaattatgac ttgactactt
      421 atagtaatca tagtatcata tttgtaatga tgtattgcat tatatgtcat aaagttaaaa
      481 atctgaatct ttcattagca ttatcatgga aatttttgca aggaatgtat tacattaaaa
      541 gcacatttta tactattttg taatatataa acataacgga gggagctatg aatataacat
      601 ccaattcgtt tgccagcggg ggaataatcc ctgtacaata tgccatgaaa ggaatacccg
      661 gcggtcagaa catctccccg catattgcaa tacatgacgt tcccaaagaa gctcgctcgc
      721 ttgctgttac ctttgttgat cgccatccca tggcacacaa ctgggtgcac tggctggtac
      781 tcaatatacc tgcaaatacc aaagaaatac aggaaggggc gtcattgaaa gctatgccac
      841 atgattccat tgagatgatc aatacttttg gttttgcagg ttatggtgga cctcaaccac
      901 ctaaaggtag cggtgtccat agctatgaac tttgctgtta tgcacttgct gaattattca
      961 agataccaaa aaaagaatta accgaaaatg aattgttaaa actcataaaa ccattgctga
     1021 ttacccagtg tacaatacac ggtaactttg aaaataaata acgttacttt ggcagtacat
     1081 gctcatgtat cataccatat aaaaagtatt gactataata tctttttctg agtgttaggt
     1141 ttattgatat acatttttac ttcatgagga atgctatggg tatcccggtt taccaatacg
     1201 atacaattat tgaagttcaa aaagaaggtg tgtgcgatag ctccgaaatc aatgcattag
     1261 ctgccattga agcacagcta aaaaatgacg aaaaaatccg ttcacttctc atcagtgaaa
     1321 catgtattaa aaaccgaact gcagcacttg caggtgatat agttcgtgac atgaatcatt
     1381 caaatcatat cgatataatg atggtgctga caggatcatt tatgtttact gccgatcttg
     1441 cacgggcatt atatgcacag ggtggtatta acacaagcat tcactgcatc aaaacaagtg
     1501 tatacgatga aaccataaaa tcaagtg
//
LOCUS       DAOSOX010000364         1525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_82706_length_1525_cov_5.117007, whole genome shotgun sequence.
ACCESSION   DAOSOX010000364 DAOSOX010000000
VERSION     DAOSOX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82706_length_1525_cov_5.117007"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PLX22_13370"
     CDS             <1..1174
                     /locus_tag="PLX22_13370"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT20932.1"
                     /translation="YIDQYAGRFNYCKVISGKLLPETEVLNSTKNYKERINKIYTMIG
                     NKTVDVSKVLPGDIGVVVKLDRTATLDTLCDPKQAVILPIIQLPHPVFSYAVQAAKKG
                     DEDKIGQIFSRITDENPTITYEYNPETHQTVLSGMGEMQLDIILKSIKEKNKIDLITS
                     EPRIAYRETITKKAEAQYKHKKQTGGHGQYGEVFIRVAPLERGKGFEFIDSIVGGVIP
                     RNFIPAVEKGLREGMERGVLARYPVVDISVELYYGSYHDVDSSEMSFKIAAIQALRKG
                     LEAAGPILLEPVMEVEIFVDKEYMGDILNDITSRRGRVLGMGSADEDGNSPISVVKAT
                     VPLAEMLRYSIDLRAMTSGKATFEMRFSHYDPISGKIAEKVIEERKKEFAEEEANK"
BASE COUNT          510 a          236 c          336 g          443 t
ORIGIN      
        1 atacattgat cagtatgcag ggcggtttaa ttattgtaaa gtgatatccg gtaagttatt
       61 gcctgaaact gaagtattga atagtacaaa aaattataaa gaacgtatta ataaaattta
      121 cacgatgatt ggcaataaaa ctgttgatgt gtcaaaggtg ttgccgggag atataggcgt
      181 ggtggtaaaa ctggatagaa ctgctaccct tgatacatta tgtgatccaa aacaggcggt
      241 tatattgcct atcatacagt tgcctcatcc agtgttctcg tatgctgtac aggcagccaa
      301 aaagggtgat gaagataaga ttggtcagat attttccaga ataactgatg aaaatccaac
      361 aataacctat gagtataacc ctgaaacaca tcagaccgtt ttatcgggga tgggcgagat
      421 gcagcttgat attatattaa aatctatcaa agaaaagaat aaaatagatt taattacttc
      481 tgaaccacgt attgcttacc gggaaacaat cacaaagaaa gccgaagcac agtacaaaca
      541 taaaaagcaa acgggtggac atgggcagta tggcgaggta tttatccgcg ttgcaccttt
      601 agagcggggt aaaggttttg aatttataga cagtattgtt ggcggagtca ttccaagaaa
      661 ttttataccc gctgtagaaa aaggattgcg tgagggtatg gaacgaggtg tgttagcacg
      721 ctatcctgtt gttgatatat cagttgaatt atattatggc tcgtaccatg atgttgattc
      781 ttctgaaatg tcatttaaaa ttgctgctat ccaggcatta agaaaaggac ttgaagcagc
      841 cggaccaatt ttacttgaac cagtaatgga agtagagata tttgtggata aagaatacat
      901 gggggatatc cttaatgata tcaccagccg cagaggcagg gtccttggta tgggaagtgc
      961 tgatgaagat ggtaattcgc caatctcggt ggttaaggct actgtaccat tagctgaaat
     1021 gttgcgatat tccattgatc tgcgagcaat gaccagtggc aaagctacat ttgaaatgcg
     1081 cttttcgcac tatgatccta tatccggaaa aattgctgaa aaagtaattg aagaacgcaa
     1141 aaaagagttt gctgaggaag aagcaaacaa ataaagaatg atttacataa ctattataat
     1201 caagccactg gtagcaggtg gcttgtttta tattgacagt cattatcaat tacactatat
     1261 tgtaactaca tgagttatac tatatgtatg taatgtcaaa aagaacattc cgtgacatgc
     1321 atggaaaagt aagtagttgt tgtcatttaa aagtaaagct gatagtcatt ttacaccaat
     1381 ggaacctgtg ctgattgaag gtaaagcaaa gaatctcttt aagtcgtcaa gttgaaggtt
     1441 accatgaaaa ttatagtttt tgaagattct cgctataatg atttttatcc ctttaccatg
     1501 actcgtcctg tatgggattt gcgat
//
LOCUS       DAOSOX010000365         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_83022_length_1521_cov_3.714870, whole genome shotgun sequence.
ACCESSION   DAOSOX010000365 DAOSOX010000000
VERSION     DAOSOX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83022_length_1521_cov_3.714870"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1102
                     /locus_tag="PLX22_13375"
     CDS             176..1102
                     /locus_tag="PLX22_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20933.1"
                     /translation="MDTTIVKFIIFQLCIWIPIVAGSYLAQHTAYNIQQLSSRLVTIN
                     LVSLEAFIVLWTTWGAELSVHYIILPFAGIVMVIMGFAAGAAVSFLLGFNAIQKHTVI
                     ISSSLANHGFTMGGFLCYLILGEHGLALSFIFITYFMPYVILVIFSYARYVGTKQPFS
                     LRNYIITPQNMPLLAIVVAIILHALNIKRPAINIPIDILLCISIIIYYFSMGIYFKFT
                     HIKNYCLLHLLIAIIKFCIIPLCMYIVLCQFSLPEDIQSVIRLQSYMPAAVYSVITSI
                     LFHLDKEMATSIFIVNTVAFSIIVLPALIIIF"
     gene            complement(1150..1326)
                     /locus_tag="PLX22_13380"
     CDS             complement(1150..1326)
                     /locus_tag="PLX22_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20934.1"
                     /translation="MCHAEGALAIEESIRFIIKRWDSSLCPEWLSALPQNDNDDISSS
                     LIGGYYLAIMNSHH"
BASE COUNT          418 a          309 c          258 g          536 t
ORIGIN      
        1 acccctttta ttggtcacct gtccctcctt gaacaggtta gctgacctca tctatagcat
       61 cggatggaaa gaggtttccc ataacatttt tttcataatg taattgacca caatcatgat
      121 tcttctatgc aattgtcata agtaatatgt tagatagtta gtatgcaaca attgtatgga
      181 taccacgata gttaaattca tcatcttcca gctatgcatc tggataccca ttgtagcagg
      241 gtcctacctt gcgcagcata ctgcatacaa tatacagcaa ctatcgagca ggcttgttac
      301 tataaatctt gtaagtctgg aagcatttat tgtgctgtgg acaacatggg gtgctgagct
      361 ttctgtacac tatattatcc tgccctttgc cgggattgtt atggtgatta tgggatttgc
      421 ggcaggtgct gcagtaagtt ttcttctggg tttcaatgct atacaaaagc ataccgttat
      481 cattagttct tctttagcca accatgggtt tactatgggc ggatttttat gttatttgat
      541 tctgggagaa catggattag cgttgtcatt tatctttata acctatttta tgccatacgt
      601 tatactggtc attttttcct atgcgcggta tgttggcacc aaacaaccat tttctttacg
      661 aaactacata attacacctc agaatatgcc gctattagct atagttgttg ctatcatact
      721 ccacgcactg aatattaaaa ggcctgccat aaacatccct atagatatat tactgtgtat
      781 ttcaatcatt atttactatt ttagcatggg tatttatttt aaatttactc acattaaaaa
      841 ctattgctta cttcatctgc ttattgccat catcaagttt tgcataattc ctctttgcat
      901 gtacatagtg ctctgccaat tttctctccc tgaggatata caatcggtta tccgcctgca
      961 atcatatatg cctgctgcag tatattcagt tattacttca atactgtttc atttagataa
     1021 agaaatggct accagtatat tcatcgtcaa taccgttgca ttttcaataa ttgtactgcc
     1081 tgcacttatc attatttttt aaataccttt gttaactggc aacactacta tcctataata
     1141 taaaactgtt caatgatggg agttcatgat tgccagatag tagccaccaa taagggatga
     1201 ggagatatcg tcattgtcat tctgaggcaa agctgatagc cattctggac acagtgaaga
     1261 atcccatcgt ttgataatga aacgaatgga ttcttcaatc gctagcgctc cctcagcatg
     1321 gcacattttc attctatttt ttcagcagtg caacgatata actacgttca atttctgtta
     1381 ctcatatata ctatatactg tgctagtgtg ctgacggata tcctactggc atgatgaata
     1441 gtggtagttc attttcatgt aatttaaatg cctttttaca ccctgtgaca ttaacacctg
     1501 caacaaggca tgtccctaac t
//
LOCUS       DAOSOX010000366         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_83161_length_1519_cov_4.393443, whole genome shotgun sequence.
ACCESSION   DAOSOX010000366 DAOSOX010000000
VERSION     DAOSOX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83161_length_1519_cov_4.393443"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLX22_13385"
     CDS             complement(<1..443)
                     /locus_tag="PLX22_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOT20935.1"
                     /translation="MALLTDKNIEVVAPAGNIEKLQFAIHYGAHAVYFGGQQFNLRAT
                     TKNFSVEDIEKAVSYAHNHQVKAIFLLNAFLHETDIQPVKEYIRLIKHMTFDAIMVSD
                     PGMALLLQEAGITCKLHLSTQMSTLNHIAIQFWQKQGISRIVLAR"
     gene            complement(415..1425)
                     /gene="mltG"
                     /locus_tag="PLX22_13390"
     CDS             complement(415..1425)
                     /gene="mltG"
                     /locus_tag="PLX22_13390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT20936.1"
                     /translation="MKQSKILVISLIIIAAIVIGIIWLFNHAPSGMQEGIITIRPGMN
                     LHQSAYYLKSKNVITSASFFIVMGRLLNETNVIAGNYRIYEGENSFSILHKITRGRFI
                     TKKVTIPEGFTMYDIATLLDSNDICSEGSFLYYAQSPAFMASIGIHAPGAEGYLFPDT
                     YLLPENSDPRDIIIRLVNQTKKVITTIKNSIDGKNVSMHTMLIIASLVEREAKINSER
                     PLIAAVIYNRLQLKMRLDCDSTVQYGLKKFGKRLTYNDLNEDTPYNTYKFTGLPPTPI
                     CNPGKQSLLAAYNPADVAYLFFVARNDGSHYFSKTLKEHSRAVDYYQKGIKNGFIDRQ
                     KH"
BASE COUNT          455 a          266 c          290 g          508 t
ORIGIN      
        1 tccctggcta aaacaatacg agatatcccc tgtttttgcc agaattgaat agctatatga
       61 ttaagggtgg acatctgcgt tgaaaggtgc agcttacagg taataccagc ttcctgtaaa
      121 agtaaagcca tcccggggtc agataccatg attgcatcaa atgtcatatg ttttataagt
      181 cgtatgtatt ctttaacggg ttgtatgtct gtttcatgga gaaaagcatt gagtaaaaat
      241 attgctttta cttgatggtt gtgtgcgtag gatactgcct tttctatatc ttcaacagaa
      301 aaatttttgg ttgttgcacg caaattaaat tgctgaccgc caaagtagac ggcatgtgca
      361 ccatagtgaa tggcaaattg aagtttttca atattaccag caggtgccac cacttcaatg
      421 tttttgtctg tcaataaagc cattttttat tcctttctgg taataatcaa ctgcccttga
      481 atgctctttt aatgtttttg aaaaataatg tgatccatca ttccgtgcta caaaaaataa
      541 ataagcaaca tcagccgggt tgtatgcagc aagtagcgat tgcttgccgg ggttgcagat
      601 tggtgttggt ggaagccctg taaatttata ggtattgtac ggtgtatcct cgttcaggtc
      661 attgtaggta agccgtttgc caaatttttt taaaccatac tgtacggtag agtcacaatc
      721 caatcgcatc tttaattgta atcgattata aatgacggca gcaatcaatg gcctttcgct
      781 gttgatttta gcttctcgtt caacaagcga tgcaataatg agcatagtat gcattgatac
      841 atttttccca tcaatagaat ttttgatggt ggtaattacc ttttttgttt gattaacaag
      901 gcggataata atatcgcgag ggtcactatt ctccggcaga agataggtgt cagggaaaag
      961 atacccttcg gcaccgggag catgaattcc aattgatgcc ataaaagcag gtgattgtgc
     1021 ataatataaa aagctacctt ccgaacatat atcattgcta tcaagcaatg tagcaatgtc
     1081 atacatggta aaaccctcgg gaatggttac cttctttgta ataaatcttc cccttgttat
     1141 cttatgtaat atagaaaaac tattctcacc ttcatatatg cgatagttac cagcaattac
     1201 atttgtttcg ttgagtaatc ttcccataac aataaagaat gaagcgcttg ttattacatt
     1261 ttttgacttg agataatatg cagactgatg tagattcatc ccgggacgta tggtgatgat
     1321 accttcctgc atgcctgatg gagcatggtt aaacaaccat atgataccaa taactatcgc
     1381 tgctataatg atgagtgata taactaatat tttactttgc ttcatgaaag cgacctttaa
     1441 ctttaaatat ttaaataaat aatttgttga cttaaattgt aaggtagctg tttaattact
     1501 ttgattttca atactatta
//
LOCUS       DAOSOX010000367         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_83718_length_1512_cov_9.401510, whole genome shotgun sequence.
ACCESSION   DAOSOX010000367 DAOSOX010000000
VERSION     DAOSOX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83718_length_1512_cov_9.401510"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PLX22_13395"
     CDS             complement(<1..1500)
                     /locus_tag="PLX22_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOT20937.1"
                     /translation="MFATGIAAIVIAGVMLFTHYFSGTMEKAVCTAVIGDVHVIRGNQ
                     SVPLMNGMGIQQRDRIICGKGSMAVIEMGSLKLRVDQNSEFAIDTVQSKGKLLLDTSM
                     THGVIYATIAKLKKGDSVFVKTKTAVASIRGTTFMIRSNNNITKLIVLEGKVMIAPRL
                     DSIPQESTPGVVVDAGQACVVDAGTIIAIKKAVSMNTTELDTIIQQSLPVTIADKKII
                     QIIETLTRDINQAIPVNENELTLNALYNIKAVVPDNNDVLVTTSDGVYYCKDYKVEWM
                     YSLPMPSKPFKWDTMYILQSKDLYAIDRKGDVLWQCGIEGEVLDDGILRIKNTIIVPT
                     SKGLIYFIDKKGRVIHRVDCNAPFITKPVLFNQMVCIVTDDGYLYAIDISLGVSIYRK
                     HIGTVIENGLIAQYPEIYFVTPDSIQKLHVLKDELVWSFNDSNIIDAIAYDTGIVYAT
                     TNGLLCKLSANGDSEWRISPGKSIHSLKSIHNGITCIADTVFYLIASNGE"
BASE COUNT          446 a          346 c          229 g          491 t
ORIGIN      
        1 ctcaccattt gaagcaatga gataaaaaac cgtatcggca atacatgtta ttccattgtg
       61 gatgcttttt aatgaatgta tactcttgcc cggactaatc ctccattcac tatccccatt
      121 ggcagatagc ttacacaaca acccattagt ggtggcatat actatcccgg tatcgtatgc
      181 tattgcatca ataatattac tgtcgttgaa tgaccatacc agctcgtctt ttaaaacatg
      241 caacttctga attgaatcag gagttacaaa ataaatttca ggatactgtg ctataagccc
      301 attttcaata acagtgccaa tgtgtttacg gtagatgcta acacccaacg aaatatcgat
      361 tgcataaagg tatccatcat ccgtcacaat acaaaccatt tgattaaata aaacaggttt
      421 tgtaataaat ggtgcattgc aatcaaccct gtgtatcacc cttccttttt tatcaataaa
      481 atatattaac ccttttgatg ttggaacaat aatcgtattt tttatacgca gtatcccatc
      541 atccagcacc tctccttcta ttccacactg ccatagcaca tcaccttttc tatcaatagc
      601 gtacaggtct ttgctttgaa gtatatacat agtatcccat ttgaaaggtt ttgatggcat
      661 aggcaggcta tacatccact ccactttata atccttacag tagtataccc catcgctggt
      721 tgtaaccagc acatcattgt tatcaggtac aacagcttta atattataca gggcattgag
      781 cgttagctca ttttcgttca caggtattgc ttgattaata tctctggtca acgtttcaat
      841 aatctggatt atttttttat cggcgatagt taccggtaaa gactgctgta taattgtgtc
      901 aagttctgta gtattcatgg aaaccgcttt ttttatagca atgatggtac ccgcatcaac
      961 gacacatgct tgccctgcat caacaacaac accaggagta ctttcttgtg gtatgctatc
     1021 caatctggga gcaatcatca ccttcccttc cagcactatt aattttgtta tattattatt
     1081 ggaccttatc ataaaagttg taccacgaat acttgcaact gcggttttag tttttacaaa
     1141 aacagaatct ccttttttaa gttttgcaat agttgcatag ataacgccat gcgtcattga
     1201 cgtatccagc aataatttac ccttgctctg tactgtatcg atggcaaatt cagaattttg
     1261 atctacccgt aattttaaac tccccatttc tataacagcc attgaaccct taccacatat
     1321 tatcctgtcc cgctgttgta ttcccatgcc attcatcaac ggtaccgact gattacctcg
     1381 tataacatgg acatctccga ttactgccgt gcatactgcc ttttccatag ttcccgaaaa
     1441 ataatgagta aaaagcatta ctccagcaat aactatcgcc gctataccag ttgcaaacat
     1501 agcaacaagc tt
//
LOCUS       DAOSOX010000368         1511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2334
            NODE_83782_length_1511_cov_4.949176, whole genome shotgun sequence.
ACCESSION   DAOSOX010000368 DAOSOX010000000
VERSION     DAOSOX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864401
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:03:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,681
            CDSs (total)                      :: 2,641
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 40
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 16 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 3 of 19
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83782_length_1511_cov_4.949176"
                     /isolate="AMR_MDS_2334"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLX22_13400"
     CDS             complement(<1..443)
                     /locus_tag="PLX22_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOT20938.1"
                     /translation="MALLTDKNIEVVAPAGNIEKLQFAIHYGAHAVYFGGQQFNLRAT
                     TKNFSVEDIEKAVSCAHSHQVKAIFLLNAFLHEADIQPVKEYIRVINHIPFDAIMVSD
                     PGMALLLQEAGSTCKLHLSTQMSTLNHVAIQFWQKQGISRIVLAR"
     gene            complement(415..1425)
                     /gene="mltG"
                     /locus_tag="PLX22_13405"
     CDS             complement(415..1425)
                     /gene="mltG"
                     /locus_tag="PLX22_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT20939.1"
                     /translation="MKEIKSLVVSLIIIVAIVIGIIWLLNHAPAGMHGDIITIRPGMN
                     LHQTAYYLKSKNVITSASFFIVMGRLLNETNVIAGNYRIYEGENSFSILHKITRGRFI
                     TKKVTIPEGFTMYDIARLLDSNDICSEGNFLYYAQSPAFMTSVGIPAPGAEGYLFPDT
                     YLLPENSDPRDVIIRLVNQTKKVITEIKNSVDGKNVSVHDMLVIASLVEREAKINSER
                     PLIAAVFYNRLRLKMRLDCDSTVQYGLKKFGKKLTYNDLNEDTPYNTYKFAGLPPTPI
                     CNPGKQSILAAYNPADVAYLFFVARNDGSHYFSKTLQEHSRAVDYYQKGIKNGFIDRQ
                     KH"
BASE COUNT          448 a          283 c          288 g          492 t
ORIGIN      
        1 tccctggcta aaacaatacg agatatcccc tgtttttgcc agaattgaat agctacatga
       61 ttgagagtgg acatctgcgt tgaaaggtgc agcttacagg tactaccagc ctcctgtaaa
      121 agcaaagcca ttccggggtc agataccatg attgcatcaa atgggatatg gttaataaca
      181 cgtatgtatt ctttaacggg ttgtatgtcc gcttcatgga gaaaagcatt gagtaaaaat
      241 attgctttta cttgatggct gtgtgcgcag gatactgcct tttctatatc ttcaacagaa
      301 aaatttttag tagttgcacg caaattaaat tgctgaccgc caaagtagac ggcatgtgca
      361 ccatagtgaa tggcaaattg aagtttttca atattaccag caggtgccac cacttcaatg
      421 tttttgtctg tcaataaagc cattttttat tcctttctgg taataatcaa ctgcccttga
      481 atgctcttgt aatgtttttg aaaaataatg tgatccatcg ttccgtgcaa caaaaaataa
      541 gtaggcaaca tctgccgggt tgtatgcagc aagaatcgat tgtttaccgg gattgcagat
      601 tggtgttgga ggaagccctg caaatttata ggtattgtac ggtgtatcct cgttcaggtc
      661 attgtaggta agcttcttac caaatttttt taagccatat tgtacggtag agtcacaatc
      721 caatcgcatc tttaatcgca agcgattata aaagacggca gcaatcaatg gtctttcgct
      781 gttgatttta gcttctcgtt caacaagcga tgcaatgaca agcatatcat gcaccgatac
      841 atttttccca tcaacagaat tttttatttc ggtgattacc ttttttgttt gattcaccag
      901 acggataata acatcgcgag gatcgctatt ctccggtagc aggtaggtgt ccgggaaaag
      961 atacccttca gcaccgggag ccggtattcc aactgatgtc ataaaagctg gtgattgtgc
     1021 ataatataaa aagttacctt ctgaacatat atcattgcta tcaagcaacc tggcaatgtc
     1081 atacatggta aagccttcgg gaatggttac cttctttgta ataaatcttc cccttgttat
     1141 tttatgtaat attgaaaaac tattctcacc ttcatatatg cgatagttac cagcaataac
     1201 atttgtttcg ttgagtaatc ttcccataac aataaaaaat gaagcgcttg ttatgacatt
     1261 ttttgattta agataatatg cagtctgatg tagattcatc ccgggacgta tggtgatgat
     1321 atctccatgc atgcctgctg gagcatggtt aagcaaccag ataataccaa taactatcgc
     1381 cactataatt atgagtgata caaccagtga tttaatttct ttcatttaaa cgctctttaa
     1441 ctttaaataa ataatgtgtt gacttaaatt gtaaggtagc tgtttaatta ctttgatttt
     1501 caatactatt a
//
